LOCUS       DADLCQ010000001        75867 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000001 DADLCQ010000000
VERSION     DADLCQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.001"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..208)
                     /locus_tag="JQG95_000001"
     CDS             complement(<1..208)
                     /locus_tag="JQG95_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886704.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDR"
     gene            complement(210..430)
                     /locus_tag="JQG95_000002"
                     /pseudo
     CDS             complement(210..430)
                     /locus_tag="JQG95_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(423..644)
                     /locus_tag="JQG95_000003"
     CDS             complement(423..644)
                     /locus_tag="JQG95_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886705.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(634..834)
                     /locus_tag="JQG95_000004"
     CDS             complement(634..834)
                     /locus_tag="JQG95_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY5886706.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            964..1143
                     /locus_tag="JQG95_000005"
     CDS             964..1143
                     /locus_tag="JQG95_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886707.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            1268..1597
                     /locus_tag="JQG95_000006"
     CDS             1268..1597
                     /locus_tag="JQG95_000006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886708.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(1647..1835)
                     /locus_tag="JQG95_000007"
     CDS             complement(1647..1835)
                     /locus_tag="JQG95_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY5886709.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(2210..3438)
                     /locus_tag="JQG95_000008"
                     /pseudo
     CDS             complement(2210..3438)
                     /locus_tag="JQG95_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            3687..4808
                     /locus_tag="JQG95_000009"
     CDS             3687..4808
                     /locus_tag="JQG95_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY5886710.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="JQG95_000010"
     CDS             complement(4857..6083)
                     /gene="pepT"
                     /locus_tag="JQG95_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY5886711.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            6333..7469
                     /gene="potA"
                     /locus_tag="JQG95_000011"
     CDS             6333..7469
                     /gene="potA"
                     /locus_tag="JQG95_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY5886712.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7453..8310
                     /gene="potB"
                     /locus_tag="JQG95_000012"
     CDS             7453..8310
                     /gene="potB"
                     /locus_tag="JQG95_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY5886713.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            8307..9101
                     /gene="potC"
                     /locus_tag="JQG95_000013"
     CDS             8307..9101
                     /gene="potC"
                     /locus_tag="JQG95_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY5886714.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            9098..10144
                     /gene="potD"
                     /locus_tag="JQG95_000014"
     CDS             9098..10144
                     /gene="potD"
                     /locus_tag="JQG95_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY5886715.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="JQG95_000015"
     CDS             complement(10300..11121)
                     /gene="cobB"
                     /locus_tag="JQG95_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY5886716.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="JQG95_000016"
     CDS             complement(11137..12048)
                     /gene="nagK"
                     /locus_tag="JQG95_000016"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY5886717.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="JQG95_000017"
     CDS             complement(12077..13321)
                     /gene="lolE"
                     /locus_tag="JQG95_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY5886718.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="JQG95_000018"
     CDS             complement(13321..14022)
                     /gene="lolD"
                     /locus_tag="JQG95_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY5886719.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="JQG95_000019"
     CDS             complement(14015..15214)
                     /gene="lolC"
                     /locus_tag="JQG95_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY5886720.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            15476..16549
                     /locus_tag="JQG95_000020"
     CDS             15476..16549
                     /locus_tag="JQG95_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY5886721.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            16677..20123
                     /gene="mfd"
                     /locus_tag="JQG95_000021"
     CDS             16677..20123
                     /gene="mfd"
                     /locus_tag="JQG95_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY5886722.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            20266..21228
                     /gene="ldtC"
                     /locus_tag="JQG95_000022"
     CDS             20266..21228
                     /gene="ldtC"
                     /locus_tag="JQG95_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY5886723.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(21311..21523)
                     /locus_tag="JQG95_000023"
     CDS             complement(21311..21523)
                     /locus_tag="JQG95_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012542232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY5886724.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            21808..22440
                     /gene="comR"
                     /locus_tag="JQG95_000024"
     CDS             21808..22440
                     /gene="comR"
                     /locus_tag="JQG95_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY5886725.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(22502..23041)
                     /locus_tag="JQG95_000025"
     CDS             complement(22502..23041)
                     /locus_tag="JQG95_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY5886726.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(23268..24572)
                     /locus_tag="JQG95_000026"
     CDS             complement(23268..24572)
                     /locus_tag="JQG95_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY5886727.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISTAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(24980..25522)
                     /locus_tag="JQG95_000027"
     CDS             complement(24980..25522)
                     /locus_tag="JQG95_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5886728.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="JQG95_000028"
     CDS             complement(25545..26570)
                     /gene="nagZ"
                     /locus_tag="JQG95_000028"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY5886729.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="JQG95_000029"
     CDS             complement(26581..27405)
                     /gene="thiK"
                     /locus_tag="JQG95_000029"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY5886730.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="JQG95_000030"
     CDS             complement(27386..28027)
                     /gene="lpoB"
                     /locus_tag="JQG95_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY5886731.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(28042..28419)
                     /locus_tag="JQG95_000031"
     CDS             complement(28042..28419)
                     /locus_tag="JQG95_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY5886732.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="JQG95_000032"
     CDS             complement(28422..28781)
                     /gene="hinT"
                     /locus_tag="JQG95_000032"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY5886733.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            29120..31305
                     /gene="fhuE"
                     /locus_tag="JQG95_000033"
                     /pseudo
     CDS             29120..31305
                     /gene="fhuE"
                     /locus_tag="JQG95_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="JQG95_000034"
     CDS             complement(31365..32798)
                     /gene="ptsG"
                     /locus_tag="JQG95_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY5886734.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(33093..33890)
                     /locus_tag="JQG95_000035"
     CDS             complement(33093..33890)
                     /locus_tag="JQG95_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5886735.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(33901..34905)
                     /gene="holB"
                     /locus_tag="JQG95_000036"
     CDS             complement(33901..34905)
                     /gene="holB"
                     /locus_tag="JQG95_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY5886736.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(34902..35543)
                     /locus_tag="JQG95_000037"
     CDS             complement(34902..35543)
                     /locus_tag="JQG95_000037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY5886737.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="JQG95_000038"
     CDS             complement(35533..36555)
                     /gene="yceG"
                     /locus_tag="JQG95_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY5886738.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="JQG95_000039"
     CDS             complement(36558..37367)
                     /gene="pabC"
                     /locus_tag="JQG95_000039"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY5886739.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="JQG95_000040"
     CDS             complement(37487..38728)
                     /gene="fabF"
                     /locus_tag="JQG95_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY5886740.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="JQG95_000041"
     CDS             complement(38815..39051)
                     /gene="acpP"
                     /locus_tag="JQG95_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY5886741.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="JQG95_000042"
     CDS             complement(39262..39996)
                     /gene="fabG"
                     /locus_tag="JQG95_000042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY5886742.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="JQG95_000043"
     CDS             complement(40009..40938)
                     /gene="fabD"
                     /locus_tag="JQG95_000043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY5886743.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(40954..41907)
                     /locus_tag="JQG95_000044"
     CDS             complement(40954..41907)
                     /locus_tag="JQG95_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY5886744.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="JQG95_000045"
     CDS             complement(41975..43045)
                     /gene="plsX"
                     /locus_tag="JQG95_000045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY5886745.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="JQG95_000046"
     CDS             complement(43126..43299)
                     /gene="rpmF"
                     /locus_tag="JQG95_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY5886746.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="JQG95_000047"
     CDS             complement(43351..43872)
                     /gene="yceD"
                     /locus_tag="JQG95_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY5886747.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            44071..44655
                     /gene="yceF"
                     /locus_tag="JQG95_000048"
     CDS             44071..44655
                     /gene="yceF"
                     /locus_tag="JQG95_000048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY5886748.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="JQG95_000049"
     CDS             complement(44767..45726)
                     /gene="rluC"
                     /locus_tag="JQG95_000049"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY5886749.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            46299..49484
                     /gene="rne"
                     /locus_tag="JQG95_000050"
     CDS             46299..49484
                     /gene="rne"
                     /locus_tag="JQG95_000050"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY5886750.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRDEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACTSPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="JQG95_000051"
     CDS             complement(49680..50633)
                     /gene="flgL"
                     /locus_tag="JQG95_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY5886751.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="JQG95_000052"
                     /pseudo
     CDS             complement(50645..52287)
                     /gene="flgK"
                     /locus_tag="JQG95_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="JQG95_000053"
     CDS             complement(52353..53294)
                     /gene="flgJ"
                     /locus_tag="JQG95_000053"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY5886752.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(53294..54391)
                     /locus_tag="JQG95_000054"
     CDS             complement(53294..54391)
                     /locus_tag="JQG95_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY5886753.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="JQG95_000055"
     CDS             complement(54403..55101)
                     /gene="flgH"
                     /locus_tag="JQG95_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY5886754.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="JQG95_000056"
     CDS             complement(55154..55936)
                     /gene="flgG"
                     /locus_tag="JQG95_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY5886755.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(56074..56825)
                     /locus_tag="JQG95_000057"
                     /pseudo
     CDS             complement(56074..56825)
                     /locus_tag="JQG95_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(56845..58773)
                     /gene="flgD"
                     /locus_tag="JQG95_000058"
                     /pseudo
     CDS             complement(56845..58773)
                     /gene="flgD"
                     /locus_tag="JQG95_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="JQG95_000059"
                     /pseudo
     CDS             complement(58785..59188)
                     /gene="flgC"
                     /locus_tag="JQG95_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="JQG95_000060"
     CDS             complement(59192..59608)
                     /gene="flgB"
                     /locus_tag="JQG95_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY5886756.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            59763..60422
                     /gene="flgA"
                     /locus_tag="JQG95_000061"
     CDS             59763..60422
                     /gene="flgA"
                     /locus_tag="JQG95_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY5886757.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            60498..60791
                     /gene="flgM"
                     /locus_tag="JQG95_000062"
     CDS             60498..60791
                     /gene="flgM"
                     /locus_tag="JQG95_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY5886758.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            60796..61212
                     /gene="flgN"
                     /locus_tag="JQG95_000063"
     CDS             60796..61212
                     /gene="flgN"
                     /locus_tag="JQG95_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY5886759.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="JQG95_000064"
     CDS             complement(61252..62787)
                     /gene="murJ"
                     /locus_tag="JQG95_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY5886760.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(62954..63820)
                     /locus_tag="JQG95_000065"
     CDS             complement(62954..63820)
                     /locus_tag="JQG95_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY5886761.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(63822..64469)
                     /locus_tag="JQG95_000066"
                     /pseudo
     CDS             complement(63822..64469)
                     /locus_tag="JQG95_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
     gene            complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="JQG95_000067"
     CDS             complement(64480..65064)
                     /gene="rimJ"
                     /locus_tag="JQG95_000067"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY5886762.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            65300..66508
                     /gene="mdtH"
                     /locus_tag="JQG95_000068"
     CDS             65300..66508
                     /gene="mdtH"
                     /locus_tag="JQG95_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY5886763.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            66572..67219
                     /gene="grxB"
                     /locus_tag="JQG95_000069"
     CDS             66572..67219
                     /gene="grxB"
                     /locus_tag="JQG95_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY5886764.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            67353..67913
                     /locus_tag="JQG95_000070"
     CDS             67353..67913
                     /locus_tag="JQG95_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5886765.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            68019..69065
                     /gene="pyrC"
                     /locus_tag="JQG95_000071"
     CDS             68019..69065
                     /gene="pyrC"
                     /locus_tag="JQG95_000071"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY5886766.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            69139..69384
                     /gene="dinI"
                     /locus_tag="JQG95_000072"
     CDS             69139..69384
                     /gene="dinI"
                     /locus_tag="JQG95_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY5886767.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            69674..69928
                     /gene="bssS"
                     /locus_tag="JQG95_000073"
     CDS             69674..69928
                     /gene="bssS"
                     /locus_tag="JQG95_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY5886768.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            70043..71161
                     /gene="solA"
                     /locus_tag="JQG95_000074"
     CDS             70043..71161
                     /gene="solA"
                     /locus_tag="JQG95_000074"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY5886769.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            71209..71322
                     /locus_tag="JQG95_000075"
     CDS             71209..71322
                     /locus_tag="JQG95_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY5886770.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            71583..72149
                     /locus_tag="JQG95_000076"
     CDS             71583..72149
                     /locus_tag="JQG95_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY5886771.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            72153..72728
                     /locus_tag="JQG95_000077"
     CDS             72153..72728
                     /locus_tag="JQG95_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY5886772.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(72770..73822)
                     /locus_tag="JQG95_000078"
     CDS             complement(72770..73822)
                     /locus_tag="JQG95_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY5886773.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            74047..74967
                     /locus_tag="JQG95_000079"
     CDS             74047..74967
                     /locus_tag="JQG95_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY5886774.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            75139..75336
                     /locus_tag="JQG95_000080"
     CDS             75139..75336
                     /locus_tag="JQG95_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY5886775.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            75360..>75867
                     /locus_tag="JQG95_000081"
     CDS             75360..>75867
                     /locus_tag="JQG95_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY5886776.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKV"
BASE COUNT        18495 a        20067 c        18765 g        18540 t
ORIGIN      
        1 atctgtcgtg caatagatct atttcttgca gttccattat tacaggctca agaagcgtta
       61 ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc attgtcgcca agtgtttcaa
      121 gccatatgcg caatatttcc agcatgtttt cactgtgaga aagtgcaaga aatgcgcggt
      181 ctattgtttc gtggtaaata tcacgcatat taatccccgt ccgtcgtttt tcttaaaata
      241 acctctgtca cgaaatcagc ataatcggcg gcgatattca gtacatcaag cccgttgatt
      301 tatattctct cgtggaaagt aagaaaaaag ccgctctaat aagttctggc attgacgaaa
      361 gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg tttcagggaa ttaaaacgat
      421 catcacgcat aacccccccc attttcacaa tcggcaacaa gaatattttt agcgtcattc
      481 agtgaccgcg ttgcgatgta tcggatacat tccagggcga actgtgtccg gtctttctgt
      541 tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa taagcgcgtg catcagcgta
      601 gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt gcataggttt ccccctgacg
      661 aatacgggcg gcgaatacag caacacaacc ggacgggcaa cggctacgcg cttcgcgttc
      721 cgtccaagcg gttacgtgga tgatttggga ttctccggca ctcagtgcca gaaaacgcca
      781 cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga tttacggcaa ccataacggc
      841 tcctagttta cgttgttggt tagaagcccc gttactgctc ctacagtgcg gggctttgct
      901 ttttgtgttg attgtgtgta atgtgtcact acacataaat acattacctg aggtgtaatt
      961 aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc cgttccagtt ccgtttagat
     1021 cctgacttac gggaaaagat ggaaaaggca caacaacagg acggcgatga ttctcttgct
     1081 gcctggataa agcgaataat ccgcaaagaa ttacagcaac gcggcataga acccaaagga
     1141 taaaaactat cagcgccgtg gtgtgaggta ctacggcgca ttgctttaca ggcgcacaca
     1201 atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac gaaaaagacg acgcagaaag
     1261 aaagagcatg aatcagaacg attcgcgccg tgttcttttg cccttgagaa gtaccttaaa
     1321 gaatactcat caaaggagag aacttcgcag ttatggcaac gcactgaacc agactgattg
     1381 cattgcccac cagcctgata gcggctatca tccccgtgct tatgtttggg atcacacaca
     1441 caaacggcgc agcgggttat ctgttcagaa aggcggctcc atttcggggc cgcttttttt
     1501 atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac cagggcgaac gaatccccgc
     1561 ccacgttctg gcgtatattc aatcttcatg gttatagctc tgtgttcaga tgattgatgt
     1621 gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct tgcagctatc cttccaggcc
     1681 agaacctcgg ataaagacca gccaacggaa cgaccgccaa gtttacgacg tgatgggaat
     1741 tgtccggcct tttccaggcg gtagcggcat gagcggctaa ggcctgttag cttttcgcat
     1801 tctttttcac gtataaaccg atcagtgctt aacactattg ccccctttcg tttcttaaag
     1861 agttatttcg tgttctattg cgttgggatg tgtctgattg tgtcaggatg attcagagtt
     1921 ggcaaatgtt gaggccgcat ggtttacaga aagaggaata atcaggataa aatcctttaa
     1981 attcatgtta atacaaaggc ataaaatatt gtttcatgcc ttttttctcg ctatttaaag
     2041 agtgattcgc tagtgtataa aaaatcagca acacattaaa aatcagctat ttataaatct
     2101 gtacactttt tcacctcttg ttcgtagttg ttccgctttg ttgctcattg ttgcacgttg
     2161 tatctgttcg catatccagt atgcgcatac tgaaaaaaca cgaaaaaaat tattttcttc
     2221 tggctactgg tagcgtggtt acgttttcat gtgttcccgc cagtatccct aaccgctccg
     2281 tccacatatc cagcgcattg cgtttagcat ccagataacg ggaatgatta taaactcgct
     2341 gcattcctgg catctggtgg cctgtaagct gctccacgac gtgaggatcc acgcctaaat
     2401 cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag tgaccagtga gggtgattta
     2461 gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt ttcctgtttc acttccccca
     2521 gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt gacgaacggc agtattgctt
     2581 ccggtatggg ccggaatatt gccaccttcg ttttgctgtg ttctttcggc acggtccaga
     2641 gcatttcggt aaaatcccac tcgctgatct ccgataacct cagttctacc gtccggcatc
     2701 cgaacacaat caggaggcgg attaacgcga tgtagtaggg ggagaatatt tttttgtcca
     2761 gtgcctgcaa taattcgccc agttctttgg tgcttaagac acgctcgctt atatccggtt
     2821 ttttcccaac gtccgccaca ctcatatcat caagaacgtt gctgattgca tagcgccgcc
     2881 tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa gaatccggca gtaacaggcg
     2941 ttcgctttgc cacctggtca aaacaggcca gccagtgccg tagctcgcat ttatccagcg
     3001 gcatagcacc aatgtgctgt attacgtgat tattaaggcg ctttttcagg gcggcataat
     3061 ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt gagcgcatcg ccaaccgtta
     3121 ccggctttaa cgcttcctgt acggtgtaat taagctcatg acgtggattt tcccctctgc
     3181 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggttca gatcaggata
     3241 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
     3301 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
     3361 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
     3421 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
     3481 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
     3541 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
     3601 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
     3661 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
     3721 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
     3781 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
     3841 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
     3901 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
     3961 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
     4021 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
     4081 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
     4141 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
     4201 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
     4261 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
     4321 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
     4381 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
     4441 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
     4501 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
     4561 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
     4621 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
     4681 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
     4741 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
     4801 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
     4861 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
     4921 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
     4981 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
     5041 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
     5101 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
     5161 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
     5221 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
     5281 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
     5341 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
     5401 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
     5461 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
     5521 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
     5581 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
     5641 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
     5701 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
     5761 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
     5821 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
     5881 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
     5941 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
     6001 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
     6061 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
     6121 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
     6181 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
     6241 aagcggtttg ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa
     6301 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
     6361 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
     6421 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
     6481 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
     6541 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
     6601 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
     6661 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
     6721 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
     6781 gtggtcaaca gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt
     6841 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
     6901 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
     6961 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
     7021 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
     7081 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
     7141 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
     7201 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
     7261 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
     7321 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
     7381 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
     7441 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
     7501 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
     7561 acccgcgacg acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg
     7621 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
     7681 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
     7741 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
     7801 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
     7861 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
     7921 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
     7981 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
     8041 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
     8101 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
     8161 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
     8221 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
     8281 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
     8341 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
     8401 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
     8461 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
     8521 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
     8581 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
     8641 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
     8701 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
     8761 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
     8821 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
     8881 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
     8941 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
     9001 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
     9061 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
     9121 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
     9181 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
     9241 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
     9301 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
     9361 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
     9421 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
     9481 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
     9541 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
     9601 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
     9661 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
     9721 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
     9781 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
     9841 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
     9901 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
     9961 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    10021 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    10081 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    10141 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    10201 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    10261 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    10321 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    10381 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    10441 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    10501 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    10561 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    10621 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    10681 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    10741 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    10801 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    10861 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    10921 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    10981 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    11041 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    11101 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    11161 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc
    11221 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    11281 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    11341 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    11401 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    11461 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    11521 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    11581 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    11641 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    11701 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    11761 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    11821 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    11881 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    11941 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    12001 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    12061 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    12121 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    12181 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    12241 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    12301 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    12361 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    12421 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    12481 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    12541 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    12601 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    12661 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    12721 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    12781 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    12841 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    12901 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    12961 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    13021 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    13081 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    13141 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    13201 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    13261 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    13321 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    13381 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    13441 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    13501 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    13561 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    13621 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    13681 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    13741 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    13801 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    13861 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    13921 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    13981 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    14041 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    14101 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    14161 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    14221 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    14281 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    14341 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    14401 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    14461 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    14521 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    14581 tcagacgcga tgcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    14641 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    14701 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    14761 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    14821 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    14881 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    14941 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    15001 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    15061 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    15121 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    15181 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    15241 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    15301 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc
    15361 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    15421 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    15481 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    15541 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    15601 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    15661 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    15721 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    15781 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    15841 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    15901 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    15961 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    16021 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    16081 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    16141 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    16201 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    16261 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    16321 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    16381 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    16441 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    16501 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    16561 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    16621 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    16681 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    16741 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    16801 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    16861 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    16921 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    16981 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    17041 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    17101 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    17161 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    17221 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    17281 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    17341 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    17401 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    17461 cattgttctt cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc
    17521 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaagctgac acgctggcgc
    17581 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    17641 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    17701 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    17761 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    17821 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    17881 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    17941 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    18001 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    18061 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    18121 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    18181 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    18241 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    18301 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    18361 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    18421 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    18481 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    18541 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    18601 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    18661 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    18721 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    18781 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    18841 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    18901 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    18961 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    19021 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    19081 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    19141 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    19201 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    19261 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    19321 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    19381 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    19441 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    19501 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    19561 aacaaagcgg ttcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    19621 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    19681 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    19741 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    19801 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    19861 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    19921 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    19981 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    20041 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    20101 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    20161 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    20221 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    20281 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    20341 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    20401 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    20461 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    20521 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    20581 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    20641 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    20701 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    20761 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    20821 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    20881 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    20941 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    21001 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    21061 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    21121 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    21181 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    21241 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    21301 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    21361 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    21421 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    21481 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    21541 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    21601 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    21661 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    21721 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    21781 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    21841 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    21901 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    21961 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    22021 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    22081 agcgtggagt ctgcgctggc tgactatttt gcggccatcg ccaactgctt taccagcaaa
    22141 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    22201 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    22261 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    22321 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    22381 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    22441 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    22501 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    22561 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    22621 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    22681 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    22741 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    22801 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    22861 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    22921 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    22981 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    23041 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    23101 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    23161 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    23221 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    23281 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    23341 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    23401 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    23461 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    23521 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    23581 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    23641 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    23701 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    23761 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    23821 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    23881 gcagtagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    23941 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    24001 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    24061 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    24121 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    24181 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    24241 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    24301 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    24361 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    24421 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    24481 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    24541 acaatcacaa tctttttcaa tggcgtagtc aacgtgaccc ccttaaattt attaaccaat
    24601 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    24661 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    24721 aagttttgtt gcttaaaaga taggtaactt taccggggga gatcaaaaaa tagagtttat
    24781 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    24841 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    24901 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    24961 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    25021 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    25081 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    25141 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    25201 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    25261 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    25321 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    25381 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    25441 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    25501 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctgctttc
    25561 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    25621 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    25681 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    25741 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    25801 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa acccagttcc
    25861 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    25921 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    25981 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    26041 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    26101 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    26161 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    26221 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    26281 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    26341 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    26401 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    26461 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    26521 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    26581 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    26641 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    26701 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    26761 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    26821 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    26881 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    26941 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    27001 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    27061 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    27121 cgcgccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    27181 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    27241 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    27301 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    27361 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    27421 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    27481 cagaggcgct ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    27541 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    27601 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    27661 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    27721 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    27781 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    27841 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    27901 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    27961 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    28021 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    28081 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    28141 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    28201 ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga
    28261 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    28321 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    28381 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    28441 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    28501 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    28561 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    28621 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    28681 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    28741 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    28801 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    28861 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    28921 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    28981 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcatt tgcagcaaaa
    29041 ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac
    29101 ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagt
    29161 catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc
    29221 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
    29281 tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac
    29341 tcaacgtgat attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca
    29401 gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga
    29461 ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata tggttgatgg
    29521 tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact
    29581 gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc
    29641 atctgcggca attaatatgg ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt
    29701 ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac aaagcccact
    29761 caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg
    29821 gctggaccgc tacaatagtg aaaagacctt cttctcgggc attgtcgatg ctgatttagg
    29881 cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta atagcccaac
    29941 ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgcgcacg
    30001 cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct
    30061 gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga
    30121 atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt
    30181 tgggccatac agtaattatg gacctggctt tgatcatgtc ggcggcaccg gttggaacag
    30241 tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg aattgtttgg
    30301 tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttcagttca
    30361 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    30421 caagccgact ggtcatcaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    30481 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    30541 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    30601 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    30661 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    30721 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    30781 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    30841 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    30901 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    30961 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    31021 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    31081 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    31141 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    31201 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    31261 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    31321 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    31381 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca
    31441 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    31501 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    31561 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    31621 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    31681 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcggtaa cagccacagt
    31741 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    31801 tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    31861 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    31921 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcacg gttttctggt
    31981 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    32041 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    32101 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    32161 atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca
    32221 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    32281 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    32341 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    32401 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    32461 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    32521 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    32581 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    32641 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    32701 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    32761 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    32821 gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    32881 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    32941 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    33001 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    33061 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    33121 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    33181 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    33241 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    33301 agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg
    33361 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    33421 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    33481 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    33541 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    33601 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    33661 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    33721 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    33781 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    33841 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    33901 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    33961 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    34021 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
    34081 ggcgaccagt cccggcacat caacattggt cacctgcgca gcgccatgat ggcgttttag
    34141 cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag
    34201 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    34261 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    34321 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    34381 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    34441 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    34501 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    34561 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    34621 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    34681 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    34741 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    34801 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    34861 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    34921 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    34981 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    35041 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    35101 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtctgggcg aaaatccccg
    35161 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    35221 tacgcctgag tggaaagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    35281 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    35341 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    35401 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    35461 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    35521 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    35581 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    35641 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    35701 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    35761 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    35821 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    35881 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    35941 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    36001 cattttcttg tgaactcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    36061 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    36121 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    36181 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    36241 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    36301 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    36361 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    36421 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    36481 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    36541 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    36601 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    36661 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    36721 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    36781 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    36841 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    36901 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    36961 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    37021 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    37081 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    37141 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    37201 gaaatcatca accgctgaca agcatcctgt agtcgcagga tatgcgccga taacaaactg
    37261 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    37321 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    37381 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    37441 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    37501 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    37561 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    37621 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    37681 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    37741 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    37801 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    37861 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    37921 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    37981 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    38041 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    38101 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    38161 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    38221 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    38281 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    38341 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    38401 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    38461 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    38521 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    38581 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    38641 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    38701 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    38761 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    38821 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    38881 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    38941 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    39001 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    39061 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    39121 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    39181 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    39241 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    39301 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    39361 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    39421 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    39481 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    39541 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    39601 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    39661 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    39721 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    39781 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    39841 gttggcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat
    39901 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    39961 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    40021 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    40081 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    40141 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    40201 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    40261 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    40321 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    40381 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    40441 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    40501 cgctttcgca ataggcgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    40561 cggtacggct tcttgcatga acttaccgcg catctcaacc agacgcaccg catcagcgaa
    40621 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    40681 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    40741 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    40801 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    40861 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    40921 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cagagcccca
    40981 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    41041 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    41101 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    41161 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    41221 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    41281 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    41341 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    41401 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    41461 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    41521 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cacaggctgc
    41581 tgcaacgtca aatgccgggc aacctttaat gtccaacatg ctttgaatct gacaagctgc
    41641 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    41701 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    41761 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    41821 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    41881 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    41941 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    42001 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    42061 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    42121 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    42181 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    42241 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    42301 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    42361 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    42421 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    42481 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    42541 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    42601 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    42661 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    42721 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    42781 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    42841 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    42901 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    42961 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    43021 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    43081 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    43141 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    43201 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    43261 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    43321 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    43381 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatgt ccgcttcgga
    43441 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    43501 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    43561 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    43621 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    43681 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    43741 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    43801 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt
    43861 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    43921 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    43981 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    44041 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    44101 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    44161 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    44221 gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    44281 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    44341 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    44401 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    44461 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    44521 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    44581 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    44641 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    44701 gacggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    44761 cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg
    44821 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    44881 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    44941 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    45001 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    45061 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    45121 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    45181 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    45241 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    45301 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    45361 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    45421 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    45481 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    45541 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    45601 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    45661 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    45721 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    45781 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    45841 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    45901 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    45961 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    46021 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    46081 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    46141 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    46201 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    46261 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    46321 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg
    46381 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    46441 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    46501 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    46561 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    46621 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    46681 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    46741 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    46801 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    46861 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    46921 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    46981 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    47041 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    47101 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    47161 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    47221 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    47281 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    47341 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    47401 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    47461 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    47521 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    47581 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    47641 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    47701 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    47761 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    47821 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    47881 cactggcaac ctttgccatg ccggatgtac cgccagcgcc aaccccagct gaacctgccg
    47941 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    48001 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    48061 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    48121 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    48181 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    48241 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    48301 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    48361 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    48421 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    48481 aagtgcgtga cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    48541 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    48601 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    48661 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    48721 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    48781 tgaccgtagc gtgcacgtct ccggaattgg cctctggcaa agtctggatc cgctatccaa
    48841 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    48901 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    48961 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    49021 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    49081 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    49141 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    49201 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    49261 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    49321 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    49381 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    49441 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    49501 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    49561 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg
    49621 gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt aaagcgagct
    49681 tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc
    49741 tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc
    49801 agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga atccagcgac
    49861 tccagtttgt tcagctgcgt gcctaattcc gcgcgaacgg taaggacatt attcagcgag
    49921 tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt atccgcttcg
    49981 ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg
    50041 gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt
    50101 ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt
    50161 tctgttccgc caacataatc accgttagcc tcactaaacg gcgcagtctc tgttttataa
    50221 ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca
    50281 cgaagcccct gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg
    50341 ctggcgtaga caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca
    50401 ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt
    50461 tgcgcctgtg cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg
    50521 ttaacgactc gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa
    50581 ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct
    50641 ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg
    50701 cattcgccag gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga
    50761 caccggaaat cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc
    50821 tactggtttt caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt
    50881 cgttaaagga tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct
    50941 gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt
    51001 cgtcggtgat taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc
    51061 tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac
    51121 cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact
    51181 gattattatc gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg
    51241 tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga
    51301 tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt
    51361 tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat
    51421 cctgagaacg gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga
    51481 tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc
    51541 tggaaggaac tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg
    51601 catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt
    51661 taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc
    51721 cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt
    51781 gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata
    51841 ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg
    51901 cgccgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat
    51961 ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc
    52021 atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa
    52081 cgtatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc
    52141 gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt
    52201 atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct
    52261 catggcgtta ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta
    52321 ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg
    52381 taggttttgc tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta
    52441 cgggcatagt gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc
    52501 gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa
    52561 tccgacaatg cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct
    52621 tcgccgtttt catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag
    52681 gctttgacac caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc
    52741 ccccaaccag attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc
    52801 tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc
    52861 agcgaatcat cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt
    52921 tgataacgca ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct
    52981 ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg
    53041 cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc
    53101 tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc
    53161 acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc
    53221 gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg
    53281 ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg
    53341 attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca
    53401 cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat
    53461 cgatctgcgt ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc
    53521 tgacattggc ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt
    53581 cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag
    53641 cgtcctgcgg ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg
    53701 aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc
    53761 catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt
    53821 cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa
    53881 taaccgcacc attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc
    53941 ctgcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac
    54001 tgtcaacgcc cttaagcggt gtcatcaaca acgtacctcc acgcaagctt ttggcattac
    54061 ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg
    54121 ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa
    54181 ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct
    54241 ggtcaccagt gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta
    54301 ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta
    54361 gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt
    54421 aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac
    54481 gtattcaata cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt
    54541 gcgtggatta accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg
    54601 tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa
    54661 cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc
    54721 ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt
    54781 atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc
    54841 gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc
    54901 ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt
    54961 ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt
    55021 ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt
    55081 atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc
    55141 caccggttat gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg
    55201 ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca
    55261 cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg
    55321 gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc
    55381 tctccagtcc ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag
    55441 ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg
    55501 cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc
    55561 cgttctgatc cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca
    55621 ttacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca
    55681 gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta
    55741 atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat
    55801 aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca
    55861 ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc
    55921 ataatgaact gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg
    55981 cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg
    56041 cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc
    56101 acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc
    56161 gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc
    56221 cgacatcaca cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc
    56281 tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac
    56341 cagtttcaga cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga
    56401 gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc
    56461 ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct
    56521 gccattacgc gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc
    56581 aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc
    56641 ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg
    56701 cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg
    56761 gttaccgcct gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga
    56821 tccattgagc tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc
    56881 ctgggttttg atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac
    56941 cagttcttta ctgagatcga cgttggacgc ttccagcgca ccgttggtca gggtgccaaa
    57001 gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac
    57061 gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc
    57121 cagcagttgg gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg
    57181 ataactcacc agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc
    57241 gccggtattt tgctgcatgg agttgaggaa gctcagacta aacgtggcgg gttctgcacc
    57301 gttaattgcg ccggttgcga tattattcgc catcgcacca tccactaatg tgccattagc
    57361 attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt ggatcactgc tatcctgggt
    57421 gtagacctgc cagttattat ccccggtctt cacaaaatag acgctcatgt catgagcatt
    57481 accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc
    57541 gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc
    57601 cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc
    57661 cccttgctga atagtcggcg gcgtaccggt taccgggtaa cccgtcagct gtaaaccttg
    57721 cgtattcaac aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac
    57781 cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc
    57841 tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac
    57901 ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa
    57961 gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc
    58021 gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta
    58081 aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt
    58141 accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct gggcaaccag
    58201 ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct
    58261 gccatcagtc aacgaaccgt cctaggtgaa actgtgaact ccggcggtca gttcaccaat
    58321 atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc
    58381 cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc
    58441 ggttcccgca agaacagttg tgccggggat catcacgccg tgaccgatca ggttactggc
    58501 ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt
    58561 ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat
    58621 tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa aactgctttg
    58681 taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt
    58741 tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta
    58801 agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg
    58861 cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg
    58921 tagccctttg catctgccag cggattacct ggttcataaa ccagtttgtc cggggcctga
    58981 ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca gcgttaacct
    59041 ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg ctatcagcat
    59101 ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac gccgacccgg
    59161 cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa acgttcatca
    59221 tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg ctgttatcgg
    59281 caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa ggctggtccg
    59341 gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg atgtgttgcg
    59401 ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc atgacttttt
    59461 taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg gcattggcga
    59521 tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct tgttgaaaac
    59581 gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa ttctgccatc
    59641 agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg gtcgctattt
    59701 atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg ctgggggcga
    59761 aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt ccgttaagta
    59821 cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc gcgggggtaa
    59881 gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca tgcgagcagc
    59941 cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg ttggcacgct
    60001 gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat tatgtggttg
    60061 ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa ctgaaacgtg
    60121 gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt gttgatgccg
    60181 ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt cgccaggcat
    60241 ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg tttagcgcca
    60301 acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg gtgcgcatgg
    60361 tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt ataaacctgt
    60421 aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat aaaagcgccc
    60481 tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct gtaagcaccg
    60541 ttcaaccgcg cgaaaccacc gacgcgccgg taacgaacag ccgggcggca aaaacaaccg
    60601 cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg caacccggca
    60661 gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt aacggtgaac
    60721 taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag caagacttgc
    60781 agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc cgctgtgctt
    60841 aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg gcagatcaac
    60901 ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac gctggattac
    60961 ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat tagtcaacgc
    61021 tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca taacggctgg
    61081 ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa accgcatcag
    61141 gaaccgacgc tatatggggc gaacggtcag acctcaacac cccatcgcgg cagtaaaaag
    61201 atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt gttacaccgt
    61261 ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga agtacgcggc
    61321 aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg tacccaatga
    61381 ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca gtaccgccac
    61441 caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt tctgcttacg
    61501 caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag aaagtgacag
    61561 cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta aaatcagcgt
    61621 aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc caggagccaa
    61681 cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg cccgctgggt
    61741 catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg ttaacggacc
    61801 tgaaagaatg cccaacgcaa ccgcactcgg cagtgccaac aggaaacaaa gacgcaaccc
    61861 ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt tcgacagcga
    61921 cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gagggaaact ccattaagcg
    61981 gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa aaatggtgtt
    62041 gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca tctgtttcac
    62101 cacgcgcatt gctccagcat cgtggaagtt aatgcgcggc aggaccagca tgccgatctt
    62161 cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa cagcccacgc
    62221 cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac caatcatgct
    62281 gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg tattcagaat
    62341 cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa tctttagtag
    62401 ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg tcaccatgat
    62461 cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa gtgtcagcag
    62521 gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac ttttatattc
    62581 cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac ggcgtaacaa
    62641 gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc caaagattct
    62701 ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca tcgagctgac
    62761 ggcggccagc gattttaata aattcatcgg tgttctaatc cggacccacg ggcaaaacgc
    62821 ctgcaaggca ggcgctgaaa gaggcgaata gtctacctag attatggtga attgctaccg
    62881 ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca acgatacgtt
    62941 gcgccagcac tgcctattcg ccggcggttt gcggaactgt ctgattttgc acgcattcaa
    63001 tgaagtgccg cgcacagccg acaaatccac gttgctcaag agtgctctgc caaccaggaa
    63061 tcggtttatt cactacaccc tgcccgcgct cctcacgcca ttcgcgcata tccgtaatgt
    63121 cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg gcacggcgat
    63181 gcatacaagt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca aacagcattt
    63241 cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg cccgacaacc
    63301 acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa agatcgtgtg
    63361 gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg gcgagttgcg
    63421 tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc aacgtcagtt
    63481 ttttccgcgc cgccagttcc accagccttt cagcatcgcg cagattttct gccagcggtt
    63541 tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca aagtggctgg
    63601 cggtgctgga gtgcacaaaa accgcatcac aactggcggc aaggctggat aacgaatcgg
    63661 cataaggaat gcgccagctg tcacaaattg gcagggcttt ggcgcgcgta ggcgaccagg
    63721 ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct ttttgcgcaa
    63781 tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc cagatgggcc
    63841 agcaacgaat caagacgttg tttcagttct gccacttcga tttccagggc ttcgacgcgg
    63901 gtctataaat caccgtcaac catgttcgac atagccgtta ccgccggctg atcttcaacc
    63961 tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg ggccagacgc
    64021 accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc cacttccgcc
    64081 atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg cgtctgggca
    64141 ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt cagatcgcca
    64201 aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa accgctcact
    64261 gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc ggattcactc
    64321 agattcatga ccggtttacg gttcgttttc tgattacagg ccgtgactac accattgact
    64381 gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc aattacgcga
    64441 gcttccaagg cagtaagttg atatttcatg agattctcct tagcggccgg gcgtccagtc
    64501 tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca acagatagtc
    64561 tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc tgcgtttatt
    64621 gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca tataacgaat
    64681 ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc caatcgaata
    64741 accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg caacgccaat
    64801 aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt gtttatgaaa
    64861 ttcgttaatc atccctagcc tggcctgcca gcctgatgga taacagtggc tttcgtcgcg
    64921 cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg ccagacgcca
    64981 ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc gcacttttgg
    65041 cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt ctttaatacg
    65101 ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa tgccattttc
    65161 tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga taaaaaaata
    65221 ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag tgctcatctt
    65281 ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg gtaaatattt
    65341 cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc cgctgatctc
    65401 tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg ctctcggtct
    65461 acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg accgctttgg
    65521 tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg ccacaatggg
    65581 tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac tcggcggcac
    65641 gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac aacaacgtgg
    65701 tcgttttttc tcgctgttga tgatgcagga cagtgccagt gcggtcattg gcgcattgtt
    65761 ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg ccgttttgtt
    65821 tgtgctatgt gcggcgttca atgcgtggtt gttaccggca tggaaactct ccaccgtacg
    65881 cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg tcacctatgt
    65941 cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc tgccaattat
    66001 ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg atgtatgcca ttgaagcgtg
    66061 tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt ttcgtctgga
    66121 acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg tgggcatggt
    66181 cagcggccta caacaacttt tcaccctgat ttgtctgttt tatatcgggt cgatcattgc
    66241 cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc gcggcagcta
    66301 tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata tcggtggtgg
    66361 ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga tgatgctggg
    66421 aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga aacgcgccgc
    66481 gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc tccatactga
    66541 aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg atcactgccc
    66601 ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg aattacatgt
    66661 tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac aggttcccat
    66721 tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc actatgtcga
    66781 taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg aagagtggct
    66841 gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca aatcggcatt
    66901 tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag aggccagcgc
    66961 gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata tcagcgatga
    67021 tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg aactttcgga
    67081 agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg gaatcaactg
    67141 gccaagccgc gttgctaatt accgcgataa tatggcgaaa cagacacaaa tcaatttgtt
    67201 atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg ggttgccgcc
    67261 gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg ttgtgtcagg
    67321 atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt gccgctgcat
    67381 tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc atcaccgaac
    67441 aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc ggtttacccg
    67501 gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt cgcgaagagc
    67561 cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg ttcggtagtc
    67621 agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa gaaaaaggtg
    67681 cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa aaaatgcaaa
    67741 cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac tttaaccagc
    67801 aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg aaaaaactgg
    67861 cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat taatgacgag
    67921 ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta tcctttgtgt
    67981 ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca tcccaggtat
    68041 taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac atgttaaaaa
    68101 ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc aatctggctc
    68161 cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt gacgccgtac
    68221 ctgccgggca cgatttcacc ccattgatga cctgttattt aacagattcg ctggatccta
    68281 atgagctgga gcgcggattt aatgaagccg tgttcaccgc tgcaaaactt tacccggcaa
    68341 acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg ccggtacttg
    68401 agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca catgcagata
    68461 tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct ctgcgccagc
    68521 gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct gccgactatg
    68581 tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg atgtttaacc
    68641 gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc atcctcaaac
    68701 gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat cgagtattcc
    68761 tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc ggctgcgcgg
    68821 gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa gagatgaatg
    68881 ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat ggcttgccgg
    68941 tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa agcatcgcac
    69001 tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg tccgttaaac
    69061 aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa taaccagtat
    69121 gttcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc tccattgcca
    69181 gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta tgcgtttcct
    69241 gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc ggttattggc
    69301 gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg ggaaagcgcc
    69361 gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg atcgccccgg
    69421 cttttttatt ttacttacga attttgagta caaaatttct acacttcctc aaaataattg
    69481 cacaatatgc taaaaaaata tgaatgacat gctgttttat tgttcatata aatcttaaaa
    69541 gcagaaattt atctgttccc ccctttaaaa attcggtgaa tacccttact tattggtata
    69601 ctgaagttgc ttcagataaa aacgcattga acctcgaata acgttgtcta gtaacacgaa
    69661 ttagggggcc atgatggaaa aaaataatga agtcattcag actcatccgc tcgtagggtg
    69721 ggacatcagc accgttgata gctatgatgc gctgatgttg cgtttgcact accagacccc
    69781 aaataagtcc gagcaggaag ggactgaagt tggtcagacg ctctggttaa ccactgatgt
    69841 tgccagacaa tttatttcga tattagaagc aggaatcgcc aaaattgaat ccggtgattt
    69901 cccggtaaac gagtatcggc gtcattaaca tgacatgacc atccacccaa aaggcacctc
    69961 accggtgcct ttaccatttc tgggttgtat atctgcattc agttaattac tctgcttaaa
    70021 ccatgagagc agagagaaaa aaatgaaata cgatctcatc attattggca gcggttccgt
    70081 aggcgctgcc gccgggtatt atgcaacccg cgccggttta aacgtgctaa tgaccgacgc
    70141 ccatatgcca ccgcatcaac acggcagcca ccacggcgat acgcgattaa ttcgccatgc
    70201 ttatggtgaa ggcgaaaagt atgtcccgtt ggtcctccgc gcgcaaacgc tgtgggatga
    70261 actttcccgc cacaacgaag atgatcccat ttttgtacgc tctggtgtca ttaacctcgg
    70321 cccggctgac tccgcatttc tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa
    70381 cgttgaaaag ctcgatgcgc aagggattat ggcccgctgg ccagaaatac gcgtcccgga
    70441 caactacatc ggcttatttg agactgattc cggttttttg cgcagcgaac tggcgattaa
    70501 aacctggatc caactggcga aggaagcggg ctgtgcgcaa ctgttcaact gcccggtcac
    70561 cgcaattcgt catgacgatg atggcgtaac tattgaaacg gttgacggtg agtatcaggc
    70621 gaaaaaagcg attgtctgcg cgggaacatg ggtaaaagac ctgctcccgg agctgcctgt
    70681 ccagcccgta cgtaaagtat ttgcctggta tcaggccgat ggccgctata gcgtgaagaa
    70741 taaattcccg gcgtttaccg gtgaactgcc caatggcgat caatattatg gttttccggc
    70801 agaaaacgac gcgttgaaga ttggcaaaca taacggaggc caggttatcc attcagcgga
    70861 tgaacgtgtt ccgtttgcgg aagtggccag tgatgggtca gaagccttcc cgttcttgcg
    70921 caatgtattg ccgggtatcg gttgctgcct gtacggcgct gcctgcacct atgataattc
    70981 gcctgacgaa gattttatta tcgataccct acccggccac gataatacac tgctcattac
    71041 cggcctgagt gggcacggtt ttaaatttgc gtcagtttta ggggaaatag ctgccgattt
    71101 tgcgcaagac caaaaaagcg attttgattt gacgccattc aggctttccc gcttccaata
    71161 atcatcatta cggcctccag aatgaggccg tttttacagg agtatttaat gcgtcggctg
    71221 ttgcactatc tcatcaataa tattcgcgag catttgatgc tttatctttt tctctgggga
    71281 ttgttggcaa ttatggatct gatctatgtg ttttattttt agttttccgc actgacataa
    71341 actgacaaat atttaatctc cctttcattt agcgtgaaaa attgaatgat attattcctg
    71401 cttctacagc actcaccaga catctctgat gctgaaccca ctaaacttat gaagattctt
    71461 tcattattaa ttcaaaatta ttgaaataaa aataaatatc attcaatttg ttatttttgt
    71521 gttgcaattc attctccgac acgccatttt ctttctctcg caaattcgta ttggatgcac
    71581 ttatgtcatt cacaaatacc cctgaacgct atggtgttat atctgcggcc ttccattggc
    71641 taagcgcaat tattgtctac ggcatgtttg ctttaggtct gtggatggtc acgctcagtt
    71701 attacgatgg ctggtatcac aaagcacccg aactgcataa aagcatcggt attttgttaa
    71761 tgatggggct ggttattcgc gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc
    71821 cgagttattc gcccatgact cgtcttgccg caaaagctgg acatctcgca ttgtacctat
    71881 tgctgttcgc catcggcatc agcggctatc tgatctcaac tgccgatggt aaaccgatca
    71941 gcgtttttgg ctggtttgac gtccccgcga cccttgccga cgctggcgca caggcagact
    72001 ttgccggtgc cctgcatttt tggctcgcgt ggagcgtcgt agtactgtct gttatgcacg
    72061 gatttatggc cctgaagcat catttcatcg ataaagacga cactctgaag cgcatgctgg
    72121 gaaagtcgtc atctgactat ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct
    72181 tcgcgtccct gatgttctct gccggttcag cggttgccgc cgattacaaa attgacaaag
    72241 aaggtcagca cgcctttgtt aacttccgca tacagcatct tggctatagc tggttatatg
    72301 gcacctttaa agatttcgac ggtactttta cctttgatga aaaaaatccg gctgccgata
    72361 aagtgaatgt gacaattaac accaccagcg tcgatactaa ccacgccgaa cgcgataaac
    72421 atcttcgcag tgcggatttt ctcaatacca caaaatatcc acaggcaaca ttcacctcca
    72481 ccagcgtgaa gaaagacggt gacgaactgg atattaccgg cgatctgacg ctgaatggcg
    72541 taaccaaacc cgtcacgctg gaagcgaaat taattggtca gggcgacgac ccatggggcg
    72601 gtaaacgtgc aggctttgag gccgaaggca aaattaagct caaagacttc aatatcaaga
    72661 cagatttagg tccagcttct caggaagtgg atctgattat ttcagtggaa ggcgtacagc
    72721 agaagtaaga gtgaaatgtt gccggatggc acgcatccgg caatgatatt tattctgttg
    72781 gatcaggaat acccagtgtt gtattcagac gtccacgaga cttattaaag atcttattgc
    72841 cattttcacg tcctgcccga cggcgtcgct gttcttctgg cggtaacgcg ctttcttcgc
    72901 agcaaatctc actacaacaa cctttgtatt tttccgcgca tactggacac tgaataaaca
    72961 gcaggtggca gccatcattt ttacagttgg tatggctgtc gcacggcgcg ccgcactggt
    73021 ggcaatgcgc gataatctca tccgatatac gttcgcccat ccgctcgtca aaaacaaaat
    73081 ttttgccaat aaaacgcacc ggcaagccct gctcgcgcgc cttacgggcg tattcaataa
    73141 ttccgccctc gatatgccag actttgttga atccgttatg tttcatccag gcactggcct
    73201 tttcacaacg aatgccgccg gtgcagtaca tgacgatttt tttatcttta tgtgcctgca
    73261 tcatctcgac tgcttttggc agctgctcac ggaaggtatc tgccggaatt tcgagcgcgt
    73321 tttcaaagtg ccccacttca tactcatagt ggttacgcat gtcgataaat agtgcatcgg
    73381 gatcgtcaag catggcgttc acttccgccg cttgcagata ctcaccaaca ttgctggcat
    73441 caaagtgagg atcgtcaata ccgtcggcaa caatgcgatc gcgtaccttc atgcgcagta
    73501 cccagaagga tttcccgtca tcatccaacg cgatattcag gcgtaaaccc tctaaagccg
    73561 ggtcgaaggc atagagctgc gcgcgaaatg tttcaacatt gctcgcaggt acgctgattt
    73621 gcgcgttaat gccctcatgc gccagataca ctcgcccaaa aacattcagc gcggtaaaca
    73681 gctgatataa agcgtcacgg gtcgccttag gatcggcgat gtggaaatac ttgtaaaacg
    73741 aaatggtggt tcgcggttcg ctctcagcca acattttggc ttttaacgcg tcgttggaaa
    73801 tgcggttgtg taacactggc atggtgtacg gttcctgcga gatgggaaag taaaaatccg
    73861 cggcatgata tagcaattat cgataattaa catccacaca ttttacgcta catttgcgca
    73921 ttaaaaatta tttgttattt acaagcgcgg caatttcgcc cagtcttcag ccacaaattt
    73981 tggttgcggg cgaaaaaatg cgacaataca tacaattgcc cgtataggtt gaaaaacagg
    74041 attgatatga cgaatctacc caagttctcc accgcactgc ttcatccgcg ttattggtta
    74101 acctggttgg gtattggcgt actttggttg gtcgtgcaat tgccctaccc gattatctac
    74161 cgcctcggtt gtggattagg aaaactggcg ttacgtttta tgaaacgacg cgcaaaaatt
    74221 gtgcatcgca acctggaact gtgcttcccg gaaatgagcg aacaagaacg ccgtaaaatg
    74281 gtggtgaaga atttcgaatc cgttggcatg ggcctgatgg aaaccggtat ggcgtggttc
    74341 tggccggacc gccgaatcgc ccgctggacg gaagtgatcg gcatggaaca cattcgtgac
    74401 gtgcaggcgc aaaaacgcgg catcctgtta gttggcatcc attttctgac actggagctg
    74461 ggcgcgcggc agtttggtat gcaggaaccg gggattggcg tttaccgccc gaacgataat
    74521 ccactgattg actggctaca aacctggggc cgtttgcgct caaataaatc gatgctcgac
    74581 cgcaaagatt taaaaggcat gattaaagcc ctgaaaaaag gcgaagtggt ctggtacgca
    74641 ccagatcatg attacggccc gcgctcaagc gttttcgtcc cgttatttgc cgttgagcag
    74701 gctgcgacca cgaccggaac ctggatgctg gcacggatgt ccggcgcatg tctggtgccc
    74761 ttcgttccac gccgtaagcc agatggcaaa gggtatcaat tgattatgct gccaccagag
    74821 tgttctccgc cactggatga tgccgaaact accgccgcgt ggatgaacaa agtggtcgaa
    74881 aaatgcatca tgatggcacc agagcagtat atgtggttac accgtcgctt taaaacacgc
    74941 ccggaaggcg ttccttcacg ctattaaatc tcccatgccg gatgcttcag aatggcatcc
    75001 ggcattacca cagcaaatcc ccctgattta gcgataaaag ctctctggat tgcgccccct
    75061 ggaagtcggg cgcataatta gtgtgtttat cttttcttct tatgttcacc gcgcctggcg
    75121 caccaacagc ggattgctat gtcaccctgt gaaaatgaca cccctataaa ctggaaacga
    75181 aacctgatcg tcgcctggct aggctgtttt cttaccgggg ccgccttcag tctggtaatg
    75241 cccttcttac ccctctacgt tgagcagctt ggtgttaccg gccactccgc cctgaatatg
    75301 tggtccggta ttgtcttcag tataatgggt tcctaaacac atcattttag gtattgaata
    75361 tgagacgggc aatcagctac atcagatttt caagtgaacg acaactgaaa ggggactctg
    75421 tacgccgaca atctaagctg gttactgact ggcttgataa gaaccctgag ttttacttgg
    75481 attcatcctt gagctttaaa gatttgggga aaagtgcttt tagtggtaag cacctgaaag
    75541 gtggtctagg tgacttttta accgctatcg agaaagggct tgttaaagct ggtgatacgc
    75601 tactaatcga gagtttagac cgtctgtcac gtcaggatat tgatattgct tctgagctat
    75661 tacgccgtat cttaagggct ggtgtagatg tagtcacact gagcgatgga gaacattaca
    75721 cacgagaaag tttaaaagac ccgttagccc tgattaaaag cattctgata atgcagcggg
    75781 cgcatgagga aagtttgaga aagtctgagc gtgtccaagc tgcatggaac cgtaaaaaag
    75841 aactcatctc cgaaggtatc aaggtat
//
LOCUS       DADLCQ010000002        72095 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000002 DADLCQ010000000
VERSION     DADLCQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.002"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1343..2938)
                     /locus_tag="JQG95_000082"
     CDS             complement(1343..2938)
                     /locus_tag="JQG95_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease glcA"
                     /protein_id="HAY5886777.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSLVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3293..5464)
                     /gene="glcB"
                     /locus_tag="JQG95_000083"
     CDS             complement(3293..5464)
                     /gene="glcB"
                     /locus_tag="JQG95_000083"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY5886778.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5486..5890)
                     /locus_tag="JQG95_000084"
     CDS             complement(5486..5890)
                     /locus_tag="JQG95_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886779.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5895..7114)
                     /gene="glcF"
                     /locus_tag="JQG95_000085"
                     /pseudo
     CDS             complement(5895..7114)
                     /gene="glcF"
                     /locus_tag="JQG95_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7125..8177)
                     /gene="glcE"
                     /locus_tag="JQG95_000086"
     CDS             complement(7125..8177)
                     /gene="glcE"
                     /locus_tag="JQG95_000086"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY5886780.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8177..9676)
                     /gene="glcD"
                     /locus_tag="JQG95_000087"
                     /pseudo
     CDS             complement(8177..9676)
                     /gene="glcD"
                     /locus_tag="JQG95_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9932..10702
                     /gene="glcC"
                     /locus_tag="JQG95_000088"
     CDS             9932..10702
                     /gene="glcC"
                     /locus_tag="JQG95_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY5886781.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10826..11967)
                     /locus_tag="JQG95_000089"
                     /pseudo
     CDS             complement(10826..11967)
                     /locus_tag="JQG95_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12329..14059
                     /locus_tag="JQG95_000090"
     CDS             12329..14059
                     /locus_tag="JQG95_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAY5886782.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14074..14970
                     /locus_tag="JQG95_000091"
     CDS             14074..14970
                     /locus_tag="JQG95_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY5886783.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15003..15251
                     /locus_tag="JQG95_000092"
     CDS             15003..15251
                     /locus_tag="JQG95_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY5886784.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15251..16422
                     /locus_tag="JQG95_000093"
                     /pseudo
     CDS             15251..16422
                     /locus_tag="JQG95_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16458..17537)
                     /gene="lptG"
                     /locus_tag="JQG95_000094"
     CDS             complement(16458..17537)
                     /gene="lptG"
                     /locus_tag="JQG95_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY5886785.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17534..18604)
                     /gene="lptF"
                     /locus_tag="JQG95_000095"
     CDS             complement(17534..18604)
                     /gene="lptF"
                     /locus_tag="JQG95_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY5886786.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18635..19195)
                     /locus_tag="JQG95_000096"
     CDS             complement(18635..19195)
                     /locus_tag="JQG95_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY5886787.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19207..20031)
                     /locus_tag="JQG95_000097"
     CDS             complement(19207..20031)
                     /locus_tag="JQG95_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886788.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20031..21380)
                     /locus_tag="JQG95_000098"
     CDS             complement(20031..21380)
                     /locus_tag="JQG95_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY5886789.1"
                     /translation="MAGFNIKHWFADGAFRTMIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21426..22184)
                     /locus_tag="JQG95_000099"
     CDS             complement(21426..22184)
                     /locus_tag="JQG95_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886790.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22216..22929)
                     /locus_tag="JQG95_000100"
     CDS             complement(22216..22929)
                     /locus_tag="JQG95_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY5886791.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23103..23795
                     /locus_tag="JQG95_000101"
     CDS             23103..23795
                     /locus_tag="JQG95_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY5886792.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23844..25343)
                     /gene="pitB"
                     /locus_tag="JQG95_000102"
     CDS             complement(23844..25343)
                     /gene="pitB"
                     /locus_tag="JQG95_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY5886793.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25635..27494)
                     /gene="gss"
                     /locus_tag="JQG95_000103"
     CDS             complement(25635..27494)
                     /gene="gss"
                     /locus_tag="JQG95_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY5886794.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27699..28565
                     /gene="yghU"
                     /locus_tag="JQG95_000104"
     CDS             27699..28565
                     /gene="yghU"
                     /locus_tag="JQG95_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY5886795.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28688..28936)
                     /gene="hybG"
                     /locus_tag="JQG95_000105"
     CDS             complement(28688..28936)
                     /gene="hybG"
                     /locus_tag="JQG95_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY5886796.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29070..29411)
                     /gene="hypA"
                     /locus_tag="JQG95_000106"
     CDS             complement(29070..29411)
                     /gene="hypA"
                     /locus_tag="JQG95_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY5886797.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29404..29892)
                     /gene="hybE"
                     /locus_tag="JQG95_000107"
     CDS             complement(29404..29892)
                     /gene="hybE"
                     /locus_tag="JQG95_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY5886798.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRSE
                     NA"
     gene            complement(29885..30379)
                     /locus_tag="JQG95_000108"
     CDS             complement(29885..30379)
                     /locus_tag="JQG95_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY5886799.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30379..32082)
                     /gene="hybC"
                     /locus_tag="JQG95_000109"
     CDS             complement(30379..32082)
                     /gene="hybC"
                     /locus_tag="JQG95_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY5886800.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32079..33257)
                     /gene="hybB"
                     /locus_tag="JQG95_000110"
     CDS             complement(32079..33257)
                     /gene="hybB"
                     /locus_tag="JQG95_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY5886801.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33247..34233)
                     /gene="hybA"
                     /locus_tag="JQG95_000111"
     CDS             complement(33247..34233)
                     /gene="hybA"
                     /locus_tag="JQG95_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY5886802.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34236..35354)
                     /gene="hybO"
                     /locus_tag="JQG95_000112"
     CDS             complement(34236..35354)
                     /gene="hybO"
                     /locus_tag="JQG95_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY5886803.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35543..35830)
                     /locus_tag="JQG95_000113"
     CDS             complement(35543..35830)
                     /locus_tag="JQG95_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY5886804.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYCLEN"
     gene            complement(35949..36875)
                     /locus_tag="JQG95_000114"
     CDS             complement(35949..36875)
                     /locus_tag="JQG95_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY5886805.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            36993..38033
                     /gene="gpr"
                     /locus_tag="JQG95_000115"
     CDS             36993..38033
                     /gene="gpr"
                     /locus_tag="JQG95_000115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY5886806.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38073..38567)
                     /locus_tag="JQG95_000116"
     CDS             complement(38073..38567)
                     /locus_tag="JQG95_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY5886807.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38758..39642
                     /gene="yghA"
                     /locus_tag="JQG95_000117"
     CDS             38758..39642
                     /gene="yghA"
                     /locus_tag="JQG95_000117"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY5886808.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39914..40339)
                     /gene="exbD"
                     /locus_tag="JQG95_000118"
     CDS             complement(39914..40339)
                     /gene="exbD"
                     /locus_tag="JQG95_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY5886809.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40346..41080)
                     /gene="exbB"
                     /locus_tag="JQG95_000119"
     CDS             complement(40346..41080)
                     /gene="exbB"
                     /locus_tag="JQG95_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY5886810.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41332..42519
                     /gene="metC"
                     /locus_tag="JQG95_000120"
     CDS             41332..42519
                     /gene="metC"
                     /locus_tag="JQG95_000120"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY5886811.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42659..43318
                     /locus_tag="JQG95_000121"
     CDS             42659..43318
                     /locus_tag="JQG95_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5886812.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43358..44257)
                     /locus_tag="JQG95_000122"
     CDS             complement(43358..44257)
                     /locus_tag="JQG95_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5886813.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44451..45614
                     /gene="yqhD"
                     /locus_tag="JQG95_000123"
     CDS             44451..45614
                     /gene="yqhD"
                     /locus_tag="JQG95_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY5886814.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45719..46546
                     /gene="dkgA"
                     /locus_tag="JQG95_000124"
     CDS             45719..46546
                     /gene="dkgA"
                     /locus_tag="JQG95_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY5886815.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46746..47672
                     /locus_tag="JQG95_000125"
     CDS             46746..47672
                     /locus_tag="JQG95_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY5886816.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47723..47980
                     /gene="yqhH"
                     /locus_tag="JQG95_000126"
     CDS             47723..47980
                     /gene="yqhH"
                     /locus_tag="JQG95_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY5886817.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48023..50242)
                     /locus_tag="JQG95_000127"
     CDS             complement(48023..50242)
                     /locus_tag="JQG95_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY5886818.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVVHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIAPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50353..51765)
                     /gene="ftsP"
                     /locus_tag="JQG95_000128"
     CDS             complement(50353..51765)
                     /gene="ftsP"
                     /locus_tag="JQG95_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY5886819.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51840..52577)
                     /gene="plsC"
                     /locus_tag="JQG95_000129"
                     /gene_synonym="parF"
     CDS             complement(51840..52577)
                     /gene="plsC"
                     /locus_tag="JQG95_000129"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY5886820.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52811..55069)
                     /gene="parC"
                     /locus_tag="JQG95_000130"
     CDS             complement(52811..55069)
                     /gene="parC"
                     /locus_tag="JQG95_000130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY5886821.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55615..56097)
                     /locus_tag="JQG95_000131"
     CDS             complement(55615..56097)
                     /locus_tag="JQG95_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5886822.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56150..56542)
                     /gene="ygiW"
                     /locus_tag="JQG95_000132"
     CDS             complement(56150..56542)
                     /gene="ygiW"
                     /locus_tag="JQG95_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY5886823.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56694..57353
                     /gene="qseB"
                     /locus_tag="JQG95_000133"
     CDS             56694..57353
                     /gene="qseB"
                     /locus_tag="JQG95_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY5886824.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAAKLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57350..58624
                     /gene="qseC"
                     /locus_tag="JQG95_000134"
     CDS             57350..58624
                     /gene="qseC"
                     /locus_tag="JQG95_000134"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY5886825.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58670..59002)
                     /locus_tag="JQG95_000135"
     CDS             complement(58670..59002)
                     /locus_tag="JQG95_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY5886826.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59321..59902
                     /gene="mdaB"
                     /locus_tag="JQG95_000136"
     CDS             59321..59902
                     /gene="mdaB"
                     /locus_tag="JQG95_000136"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY5886827.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59933..60247
                     /locus_tag="JQG95_000137"
     CDS             59933..60247
                     /locus_tag="JQG95_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY5886828.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60295..62187)
                     /gene="parE"
                     /locus_tag="JQG95_000138"
     CDS             complement(60295..62187)
                     /gene="parE"
                     /locus_tag="JQG95_000138"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY5886829.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62216..62797)
                     /gene="yqiA"
                     /locus_tag="JQG95_000139"
     CDS             complement(62216..62797)
                     /gene="yqiA"
                     /locus_tag="JQG95_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY5886830.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62797..63624)
                     /gene="cpdA"
                     /locus_tag="JQG95_000140"
     CDS             complement(62797..63624)
                     /gene="cpdA"
                     /locus_tag="JQG95_000140"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY5886831.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63649..64071)
                     /locus_tag="JQG95_000141"
     CDS             complement(63649..64071)
                     /locus_tag="JQG95_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY5886832.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64072..64701)
                     /gene="nudF"
                     /locus_tag="JQG95_000142"
     CDS             complement(64072..64701)
                     /gene="nudF"
                     /locus_tag="JQG95_000142"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY5886833.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64906..66387
                     /gene="tolC"
                     /locus_tag="JQG95_000143"
     CDS             64906..66387
                     /gene="tolC"
                     /locus_tag="JQG95_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY5886834.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66535..67206
                     /locus_tag="JQG95_000144"
     CDS             66535..67206
                     /locus_tag="JQG95_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY5886835.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67212..68372
                     /locus_tag="JQG95_000145"
     CDS             67212..68372
                     /locus_tag="JQG95_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY5886836.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLSEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68410..69225)
                     /gene="ygiD"
                     /locus_tag="JQG95_000146"
     CDS             complement(68410..69225)
                     /gene="ygiD"
                     /locus_tag="JQG95_000146"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY5886837.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPVAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69341..70114
                     /gene="zupT"
                     /locus_tag="JQG95_000147"
     CDS             69341..70114
                     /gene="zupT"
                     /locus_tag="JQG95_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY5886838.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70172..70342)
                     /locus_tag="JQG95_000148"
     CDS             complement(70172..70342)
                     /locus_tag="JQG95_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886839.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70603..71256)
                     /gene="ribB"
                     /locus_tag="JQG95_000149"
     CDS             complement(70603..71256)
                     /gene="ribB"
                     /locus_tag="JQG95_000149"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY5886840.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71360..71508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71570..71920
                     /gene="ubiK"
                     /locus_tag="JQG95_000150"
     CDS             71570..71920
                     /gene="ubiK"
                     /locus_tag="JQG95_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY5886841.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT        18030 a        19166 c        17748 g        17151 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctattttta
      481 gcaggaaata ctcaggtgtc aacattcgaa ctccaactct gaaatattca tataagttaa
      541 tgattttaat gattttaatg attttaatga ttttaatgat tttaatgatt ttttcattta
      601 ttcacaccta ataaaagtat tcaaattcag atgatttttc tgctcaacag acacaaacag
      661 gcaataatac tctccaaagc gaacaatatt tggcattacg atttaatgct tcataatcac
      721 caaattattt aaccataaat tttcttattt ctaacaaaat gattggtgtg ttgttggagt
      781 taaaatacat acataatatt aacatctgcc aatatttcac tttccgcaag acggatgatt
      841 tcgaatcctc atccacgtta ccctatccca aatatcttta ttaataggca gtaacgaaca
      901 ctgttatagc aagattaact tattaaccta acggcatctt ttatgtaaca agtggagata
      961 aattgcttaa aattgatcct cgacaaatct taaaagttct cattattctg caaaatcagc
     1021 tttgcgaata atcttatgtt tttgggagtt ttgggaattt tgatctactc attgttctag
     1081 tgaaaaatat aaataagaga aaaaacgcca ttcgtcatga aagcaacaga tgaagcaatg
     1141 taataacata ctaaaattga tctcatgaaa gtgggtactt tataaccagc acagaagcca
     1201 agttgtataa atattaaaca attcgcatcg taaaacggag actacgtata aagaagccaa
     1261 tcgtaaaaac ggttccctgg cgagataaac gacagacaaa tggaaagccg ggtgatattt
     1321 accttcatcc ggctttattg atttacgaga ctaacatccc ggtaaacaca tacgcctgca
     1381 gcagggtgat aacgccgata acgctggcaa aaatcagact gtgcttcacg gtgtagcgga
     1441 acagttcaga ttctcggccc accatgcccg tcgcggcgca ggccacggcg atagattgcg
     1501 gggagatcat cttgccagtt acgccgccgc tggtatttgc tgccaccagc agagtgtcag
     1561 agacgttgat ttgctgcgcc gtggtcgatt gcagtgaacc aaacagggcg tttgaagaag
     1621 tgtccgaccc ggtaaggaat acgcccagcc agccgagaaa cggtgagaaa aacgggaaca
     1681 tcacgcctgt acctgccagt accagcgcca gcgtggtgga catgccggaa tagttggtga
     1741 cgaaggcgaa tgccagcacc atgccaatcg acaatatcgg ccacttcaga ctaattagca
     1801 tttcggcaaa gacgccaatc cctttcttga tgcccacgcc gaggatgaag atagagataa
     1861 tcgccgcaat aaaaatagcg gtgccgccag ccgagagagg gtcgaattta aacaccgcat
     1921 ccattggcgt tggttgggcg acaatgggtg ccgctttcaa cacttgttga tgcaaatgag
     1981 ggatctggaa attaatcacc agtgaataaa acgcgccgcc cggagcaaat aacgctttaa
     2041 acggcttcat ggtccagatg gtgaccagca ccgttaagat taaaaacggt gaccacgcac
     2101 gaatgatttg ccccagacta tattctgaag gcacgggacc gccagaagat ggcttattta
     2161 ccaccatcgc acctgcggat tgccccatgc tgattgccgt ttcggtattt ttcggccgcc
     2221 agactttaag aaataaagcg agtgagacga tactcaccag cgccgaagta atatccggca
     2281 gttccggacc aatatagtta gaggtaaaga actgagtgac agcgaagctt cccccagcaa
     2341 ccagcgccgc tggccacgtc tctttcaccc ctttccagcc gtccatcatt gctaccagcc
     2401 agaacggcac aagaaccgac aggaacggta actgacgtcc cgccattgcg ccaatgtgga
     2461 acggatcgat tcccgttacc tgaccggcga ccagaatcgg cacgcccaac gcaccaaacg
     2521 ccaccgtcgc accaaagcca gcagctcctt ccagcaacgc accaaaggag aaaccaatca
     2581 gtaacacctg caaacgctga tcgtcggtga tggagataac caagctgcgg ataatatcga
     2641 actgcccgct ggcaacggtt aatttataca ggaacaccgc cgcgacaata atccacgcaa
     2701 ttggccataa tccataaata aagccatagc ccgcagcagc aaatgccata tcaatcggca
     2761 ttttaaaggc gaatatcgca atcaggatag ataatataag ggttattgct ccagcgacat
     2821 gtcctttcag acgtaatacc gcgagtgcaa cgaagaagaa tattatcggg atcagggcga
     2881 ccagagcgga tagccccagt cctcccatcg gcatatacat ttgggtccag gtaaccatat
     2941 tgttcagtct cttattatct ttatatgctt gatatactta aggttgtaat aagcaaaaga
     3001 ggactgaact gtaaaatata ggcgttatac tttacagcaa cagtacgccg ctaacgcaat
     3061 tgctacctct ggcataacaa gtatatcggg taagggtttc tgttccgcac acgcaggcgc
     3121 agagtatcgt taagatgtcc atattgttgt tttaggcccg ctagtaatgc gctacgggta
     3181 tttaatattg ttaaaccctg ataatcgctc cggttatttc cgggataaat gtactaccgc
     3241 agttactatc atagccccga caataaaact tgccggggct tttttgacgc tattaatgac
     3301 tttctttttc gcgtaaacgc caggcgtgta ataacggttc ggtataaccg tttggctgtt
     3361 tcacgccgag gaagattaaa tcgctggcag ctttaaaagc acacgagtta gcgaaattcc
     3421 ccgccatcgg acgataagcc ggatcgccag cattttgctg atcaaccact ttcgccatat
     3481 tctccagcga cgcctgcacc tgttctttgg tcagaatacc gtgacgtaac cagttggcga
     3541 tatgctggct ggagatacgc agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat
     3601 ccggcacttt tgaacaacca atcccctgct ccacccaacg caccacgtac cccagaatcc
     3661 cctgcacgtt gttatccagc tcttgttgga tctcttgcgc cgaccagtta gcgttttcag
     3721 ccaccggaat agtcagcaga tcgtccagca gcggttcaaa ttcagcactg aactcggtct
     3781 gggcaatgtt ggcttgcacg ctctgtacgt tggtatggtg gtagtgcagc gcatggagcg
     3841 tagcagcggt tggtgacgga acccaggctg tgtttgcccc ggcacgcagt tggtcgccct
     3901 tctggctgta catgtctgcc atcaggtccg gcattgccca catgccttta ccgatttgcg
     3961 ctttaccgcg cagcccacag aacagaccgg aaagcacgtt attacgttcg taggctttga
     4021 tccacggcgt cgatttcatc tggtttttac gcagcatcgg gccagcttcc atcaccgaat
     4081 gcatttcatc gccggtacgg tcgaggaaac cggtattgat gaacgccacg cggttgcgcg
     4141 cctgagcgat acagctacgc aagttcagcg aggtccggcg ttcttcatcc ataatgccca
     4201 ttttcagggt attcggtgcc ataccgagca ttgtctcaat gcgggtaaac agtttgttgg
     4261 cgaacgccac ttcctgcgga ccgtgcattt tcggtttcac aatatagacg ctgccagtgc
     4321 gcgagttttt ctgcactttt aaatcataga gggcaatcgc gccagtcatg acgccatcaa
     4381 gaataccttc cgggatttca ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat
     4441 gacccacgtt gcggataaac agcaacgagc gtccgtgcag agaaatttca gagccatcgg
     4501 cggcggtgta ctgacgatcg tcattcagtt tacgtacgat ttgccgaccg tttttctcca
     4561 ttttctcttg cagagtcccc tgcatcaggc ccagcagatt gcggtacagc aggattttat
     4621 cttccgcatc aaccgccgcg accgaatctt cgcagtcgag aatggtactg atagcagctt
     4681 cgacgataac atcgttgatg tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa
     4741 tttgcagttc aatatgcagg ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat
     4801 cgccacggta accgacaaac tgtgctggag tacgtaacgt ggtttcttta ccatttttca
     4861 actggatgcg taattgttta tcgaccacct taaacgccac cacatcctga tagctgccgt
     4921 tttccagcgg tagtgattca tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc
     4981 gctgcggatc gtagccactg accatcgccc cttcctgcgg gatgatgtcg ctgccgtata
     5041 acgcatcgta cagtgagccc cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca
     5101 ttgccggaac caccagctgt ggccccgcct gactggtgat ttcgctgtca atgcccgtgg
     5161 tttccaccgt cacgcgctcc ggttgcggca ccaggtagcc cagttcacga aggaaagatt
     5221 tataggccgc tttatctttt accggccccg gattgctgcg atgccactca tcaagcgccg
     5281 cctgaatgcg atcgcgttct gccagcaact gacgattttc tggtgccaga tcgtgaacga
     5341 tctcatcaac attgtgccag aatgcagcag cgtccagttc cacgcccggc aaaacttctc
     5401 catccacaaa acgtttaaaa ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac
     5461 tcattgtttc tctcctcgtt ttcgcttatt tcgccaacac cgctgctgcc actttcgcga
     5521 cctgcgcatc ctgtgctccg gttaaaccag aaacgcccac ggcaccaata atttgcccat
     5581 caacaacaac cggtacgccg ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg
     5641 tacgtccgtt gttcaccatc tcttcatagc ccttagtttc acgacgcccc agcgcggcgg
     5701 tacgcgcttt ctcctgggag atgtaagccg caatcggcgc gcaatcgtcc atgcgactta
     5761 acgccagcag atgaccgccg tcatcggcaa cagcaatgga aacagaccag ttatttttct
     5821 gcgcctcttc ctgacctgcg gcaataattg cactcgccat ttgctggcta agaatgactt
     5881 tagttttcat ttttttattc cttttcaagg gcttgttcta caatttcaat ccagtgacgc
     5941 acagaggtac gaccggcgct cgccagatgc gtctggcaac caatgttggc ggtgacgatc
     6001 atttccggtt tgccgctttc cagcgcattc attttgttat cccgcagctg gcgtgccaga
     6061 tcgggatgcg ttaacgcata tgttcccgct gaaccgcagc acagatggct gtcgggaacg
     6121 tccgttaagg taaatccaag acgaagtaac actttttcca cttcgccgtt cagcttttgc
     6181 gcatgttgta gggtacacgg gcagtggaag gccagttttt tatcgccgcg aattgccagt
     6241 ttttccagcg gttcctcgcg cagaagttcg actaaatcga ccgccagttc actgacctga
     6301 cgcgctttat cggcatataa cgcatcgttt ttcagcatct gcccatactc tttgacgaac
     6361 gcgccgcaac cgctggcggt ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac
     6421 caggcatcaa tattattgcg cgcccgtgcc aaccctttct cctgcgcatt aagatgatag
     6481 tccaccgcgc cacaacagcc tgcttcgtta gctgacatga cgctgatccc cagacgatcc
     6541 agcactcgcg cagttgccgc gttggtgttg ggcgaaagcg taggctgggc gcagccttcc
     6601 agcattaaaa cccgacgctt atggcgcagc ggcggacgcg gtttagcttt caccgtttca
     6661 gcaggcagtt ttgctctgac ctgttccggt aaaaacggtc gcagcaccag ccctacctgc
     6721 gtcagcgcac ggaagaccgc cggacgcggc actacctggc gcaatccttc gcgcagcatt
     6781 cgctccggca gtgggcgttt cactttctgc tcgacaatat cacgcccgat atccagcaaa
     6841 ttgtgatagc gcacaccaga aggacaggtg gtttcacaat tgcggcaagt gaggcagcga
     6901 tcgagatgct cctgtgtttt aagcgtgact tcgttgcctt ccagcacctg tttaatcaga
     6961 tagatgcgcc cgcgcggccc gtccagttca tcgcccagaa gctgataggt tgggcaggtt
     7021 gcggtacaaa atccgcagtg aacacaggcg cacaggatgc tgtcggcttc cagcgcgcgt
     7081 tctgccgcat ctcttcggtt aatcgggttt gcatagcctg ctcctcaaag ttccgcgtac
     7141 atgcgaccgg ggttaaacac gccgcaaggg tcgagctgct gtttaagctg ctggtgatag
     7201 cggaataaag gagccgatag cggggcaaag ccaccatctc cggcactaaa gcgggtcgca
     7261 tgaccgccag cgttgcgggc gatgcgatgg atttgattgt catcggctgt cgatttcagc
     7321 cagcgtaacg ccccgcccca gtcgatcagt tgctcgccgg gtaaatccat catcggcgca
     7381 tcgctgggta atgaaatgcg ccataaggta cctggtaacg agaagaacgg cagttgttgt
     7441 tcacgcaatt gctgccagaa ctgacccgca acctcttcgc cacccagcag ttcacgcgct
     7501 gcttttaccg atccttcgcc gccctcaagg cggatccaca acgcattgtc gaagtaacat
     7561 aagccactaa tgggtaatgg ctggagttgc cactcggcga tttcactcat ggcttcttgc
     7621 aggctgattt cccgacgcag gctcagagag gcacgcggtc gcggtaacac tttcattgag
     7681 atttcagtga gcacgccaag acaaccatag cttccggcca ttaaccgtga gagatcgtat
     7741 ccggcaacgt ttttcatcac ttcgccacca aaacgcagat gttttccagt gccggtaatg
     7801 atgcgcgtgc cgaggacaaa atcgcggacc gaaccgctcc acgggcgacg cggccccgcc
     7861 agcccgcagg cgaccatccc gccccaggtg gcttcttcac cataatgcgg cggctcgcag
     7921 ggaagcattt gcccggcgct ctccagcgcc gcttcaatcg ccaccagcgg cgttccggca
     7981 cgagcggtta tcaccagctc ggtcgggtcg taattaacaa tgccgcgatg acaacgcaca
     8041 tccagcgttt gcccggtgac tgggcgacct aaaaaggctt tgctattgct gccctgaatc
     8101 accagcggcg ttttatcgct aattgcctga ttcacctgct ccagcagcgc ctgactgtaa
     8161 tcacactcgc gtagcatcaa aagcgctcca gttcaggaaa aggtaaatga ccgtgatgca
     8221 catgcatggc accaaattca gcacagcggt gtagcgtggg aatgtttttc ccagggttca
     8281 gcaaaccatc ggggtcaaac gccgccttga ccgcatggaa ggtcgtgatt tcatcgctgt
     8341 tgaactgggc gcacatttga ttgatttttt ctcgcccgat gccatgttcc ccactgatgc
     8401 tgccgccaac ttcaacgcag agttcgagga tcttcccgcc cagctcttcc gcgcgggcaa
     8461 attcaccagg ttcgttggca tcgaaaagga ttaacgggtg catgttgcca tctccggcat
     8521 gaaagacgtt ggcaacacgt aaatcatatt gctgcgataa acgggcaatg ccttccagta
     8581 cgccaggcag ggcgcgacgc gggatggtgc catccatgca gtagtaatcc ggggagatac
     8641 gtcctaccgc cgggaacgca tttttgcgac cggcccagaa acgtacgcgc tctgcttcgt
     8701 cctgtgccaa acggacgtca gtcgcgcccg ctttcaacaa gatgtagtta acccgctcgc
     8761 agtcttcctg tacgtcagac tccacgccgt ccagttcgca taacaaaatc gcttcggcgt
     8821 cgacgggata accggcatga ataaaatctt ccgccgcgcg gatcgacagg ttatccatca
     8881 tctccagccc gccggggata atgccattgg cgatgatgtc accaaccgca agtccggctt
     8941 tttctaccga gtcaaagctg gctaacagaa cccgcgccac gggcggcttc ggcagcagtt
     9001 ttaccgtcac ttcggtggtc acgccgagca taccttccga tccggtgaac agcgccagca
     9061 ggtcaaaacc aggtgaatcc agcgcgtccg atcccagcgt cagcgcctcg ccgtccagcg
     9121 tttgcacttc aattttcagc aggttatgta cggtcagacc atatttcagg cagtggacgc
     9181 caccggcatt ttccgccaca ttgccgccaa ttgaacaggc gatttgtgag gaaggatccg
     9241 gcgcgtaata gagattatgc ggcgcaacgg cctaggagat cgccaggtta catacgcccg
     9301 gctgcacgcg cgcgcggcga ccaacggggt taatgtcgag gctctcttta aagcgcgcca
     9361 tcaccaacaa cacacctttt tccagcggca gcgcgccacc agaaagcccg gtgcctgcac
     9421 cacgggtcac caccggtaca cgcaggcgat ggcagacagc cagaatcgct gtcacctgtt
     9481 ccatttgctt aggcagaaca accagtaatg gacgcgtgcg atacgcgctc aacccgtcac
     9541 actcgtaagg aatgatctcc tcatcggtat gcaggatctc aagtccaggg acatgctcac
     9601 gcagtgccat cagtaccgat gtgcggtcga catcgggtaa agcgccatca agacgctctt
     9661 cgtacaagat gctcatgagt aggcttcgct ttgttgtgtt gtgttgtgtg gcagctgatt
     9721 tttgcgcgct gcttctgtga acagttatta agcgggcttt tcgttttcgt ctatctcttt
     9781 agctaccggt cagaccattt tttttccagc tctgtgacct tgtcttggtt aacttaatgt
     9841 taaattgatg taacataatc acttgcgtga tgtgcgtgtt ttgcgagtta agaacagaaa
     9901 aattggtcct acctgtgcac gaggtccggg aatgaaagat gaacgtcgcc ctatttgcga
     9961 agtggttgca gagagtatcg aacggttaat tatcgacggc gtactgaagg tcggtcagcc
    10021 gcttccctcg gaacgtcgac tgtgtgaaaa gctcggcttc tcacgctccg cactgcgtga
    10081 agggctgacc gtgctgcgcg ggcgcgggat tattgaaacg gcgcagggtc gcgattctca
    10141 tgtcgcacgg cttaatcggg agcaggacac cagcccgctg atccatctgt tcagtacgca
    10201 gccgcgaacg ctgtacgatc tgctcgacgt tcgcgcatta ctggagggcg aatcggcaag
    10261 gctggcggca acgctgggaa cgcaggctga ttttgttgtg ataacccgct gttatgaaaa
    10321 aatgctcgcc gccagtgaga accacaaaga gatttcgctg atcgaacatg cgcagttgga
    10381 tcacgctttc catctcgcca tttgtcaggc ttctcacaat caggtgctgg tgtttacgct
    10441 gcaatcattg accgatctga tgtttaattc agtgtttgcc agcgtaaata atctctacca
    10501 tcgaccacag caaaaaaagc agatcgatcg ccagcatgcg cggatctaca acgcggtgtt
    10561 gcagcggctg ccgcacgtcg ctcagcgcgc agcgcgcgat catgtacgga ccgtgaaaaa
    10621 gaatctccac gatatcgagc tggaaggcca ccatttgatt cgctcggcgg tgccgctgga
    10681 gatgaacaag gtgggaatgt gagcagagtt ttaccctatc cccaaggcga ggggaccgcc
    10741 cctgcactta acaggatctg gtgttaaatc tgttcatcta taccctctcc cgaatgaccg
    10801 cccctgcgct aaacaggatc tggtattaaa tttgcttttc atacaatcgg taacgcttgt
    10861 acggctccgc cccaatgcgt tccagcatgt tattcatgcc tgtattggtt tcgaggatcc
    10921 atgacatctc cagcgcatcg atcttccggc gggcaaacgg atcgcgtaag gcttcaatca
    10981 ataacagcgc aatcaccggg ccgatgcggc tgaactgata ctcgtcgcgc acgcccatca
    11041 gcggtactcg cgcagttcgc acaccgctga ctttcaaacg ccacagcaat tttgcccagc
    11101 cgaagggaaa gagcgatccg ttcagatcgg caatcgcctc gttgatgtta ggcaagccac
    11161 aataaacgcg cagggtgcag aatcaatctc agcgatgtag atcatatcgt ccggcaccag
    11221 atatttaagt tggtcgccca tggtcgcgaa ttcatgttcg gtaaacggca caaatcccca
    11281 gttgtgctgc cagccagagt tgaaaatctc acgcaggatc tgcatctctt cggcaaaccg
    11341 ctgacgattg atgcagcgaa tggtcacctt tttgcgcacc tgatccatca gtttttttag
    11401 cgccggagag aaagtgagat cggttcgctg catccaccac gccagtaaat caatgccttt
    11461 gtgataaccc agttgttcaa tatgcgcggc ataccacggt ttgccgtgtg gcatcatcgc
    11521 acagggtggt gtgtcaaaac cttcaatcag taatccgctt tcctgattga tattcaggct
    11581 gaaaggcccg ctgatcttac ttgcaccttg tgacttcaac cacgcttccg ctgcgccaaa
    11641 caacgcggca aaatcctgcg gatcatcaat ggcgtcaatc atgccgaaat gaccggtatc
    11701 ttcaccgtaa cgctcacggt gcaaggtatc tatttgcgcg gtaatacgcc caactatctg
    11761 ccccgctttt tttgccaccc acgcctgcca gatgatatgg tccgtccccg gatttttcgc
    11821 agacaaatgc tcgttgcgtt caatgaataa agggggtatc cagtttggat cgtcgggata
    11881 aagtgatgac ggaaaagcga taaatgcctt aaggtcattt ttattaatta ctttttcaat
    11941 tttaatcatc agcagatcgc attccatatc tctgtggata acaaataatt aatggtgaaa
    12001 aacaggttgc aacagcaacg agcggcatct taataatcaa ctcaatgaat acaaacgacg
    12061 aataaatagt attaattaat gattatgata aggtcagagg ctacacagaa acgcctcaat
    12121 gccttttgtc aatttaaggc gaccggacaa ggcttcttct taatatattc ataaattaaa
    12181 tatcattcat aataaaaaca aaaaacttaa actcattatt taaatcaatg ttcattttca
    12241 caccgatgaa aatactatct catttatctt ttatgcctat ataatccacc gcattattgt
    12301 cattttattt ttgatatcca attttttcgt ggtgtatatg tctaataaaa tctttacgca
    12361 ttccctacct atgcgctatg ccgattttcc aacgctggtt gatgctttgg actacgccgc
    12421 tctgagtaga gccggaatga atttttatga cagacgttgc caacttgaag atcaactgga
    12481 atatcagaca ttaaaaacgc gtgccgaagc tggtgcgaag cggttgttat cgctgaacct
    12541 gaaaaaaggc gaacgcgtgg cgctgattgc cgaaacgagt agcgggtttg tagaggcttt
    12601 ttttgcctgt cagtatgctg gcttagtcgc cgtcccgttg gcgattccaa tgggcgtcgg
    12661 tcagcgggat tcctggagcg ccaaactcca gggtttactg gcaagttgcc agcccgcagc
    12721 cattatcact ggtgatgagt ggttgccact ggtcaatgcc gcgacgcata acaaccccga
    12781 attacatgtt ttaagccacg cttggtttaa ggcattaccg gaagccgatg ttgcgctcca
    12841 gcgtccagtt ccgaacgata tcgcctacct ccagtacacc tccggcagca cccgttttcc
    12901 ccgtggcgtc attatcaccc atcgcgaagt gatggctaat ctacgtgcta taagccacga
    12961 tggcattaaa ttacgcccta gcgaccgctg cgtctcctgg cagcctttct accatgatat
    13021 gggactggtc ggctttctcc tgccccccgt cgccacgcaa ctttcagtag attatttgcg
    13081 cactcaggat tttgccatgc gtcctttgca atggcttaaa ttgatcagta aaaatcgcgg
    13141 caccgtttcc gttgcgccgc cgtttggcta tgaattgtgc caacgccgcg tgaatgaaaa
    13201 agatctcgct gaactggatc tttcctgctg gcgcgtcgct ggtattggtg ctgagccgat
    13261 ctccgcagaa caactccatc aattcgctga atgtttccgt caggttaact ttgacaataa
    13321 aactttcatg ccgtgctacg gactggcaga aaatgcgctg gctgtcagct tctctgatga
    13381 agcctccggg gttgtggtta acgaagtgga tcgcgacatc ctcgaatatc agggtaaagc
    13441 cgtcgcgccg ggtgcagaga cacgcgccgt atcgactttc gtcaactgcg gcaaagcgtt
    13501 gccggaacat ggtattgaaa tccgcaatga agcaggtatg ccggtcgcgg aacgtgtggt
    13561 aggccatatt tgcatctccg gtcccagtct gatgagcggt tactttggcg accaggtttc
    13621 gcaagacgag attgccgcga cgggctggtt agacaccggc gacctcggtt atctgctgga
    13681 cggttatctg tatgtcaccg gacgcattaa agatctgatt attattcgtg gccgtaatat
    13741 ctggccgcag gatattgaat atatagcgga acaggaaccg gaaattcatt ctggcgatgc
    13801 gattgctttt gttaccgccc aggaaaaaat cattttgcag atccagtgtc ggatcagcga
    13861 cgaagaacgt cgcgggcagc ttatccacgc gctggcggca cggatccaaa gcgaatttgg
    13921 cgtgaccgcg gctatcgatc tgttgccgcc ccacagtatt ccccgaacgt cctccggcaa
    13981 gcctgcccgt gcggaagcga aaaaacgtta tcagaaggct tatgctgcca gtcttaatgt
    14041 gcaggaatcc ctggcataaa tcaaactgtc gcggtgacgg gcgctaccgg gtttatcggt
    14101 aaatatatta tcgataacct gctcgcccgc ggctttcacg ttcgcgcatt gacgcgtact
    14161 gcccgcgctc acgtcaatga taatctcacc tgggtgcgcg gttcgctgga agatactcat
    14221 tcacttagcg agctggttgc cggagccagc gtagtggtcc attgcgccgg gcaagtgcgc
    14281 gggcacaaag aagagatttt cacccgctgt aacgttgacg gcagcctgcg cctgatgcaa
    14341 gcagcaaaag agagcggctt ttgccaacgt tttctgttta tctcttcgct ggcggcgcgc
    14401 catcccgagc tctcctggta tgcaaaatcc aaatacgtcg ccgagcaacg actggcagca
    14461 atggcggacg aaattacgct gggcgttttt cgcccgacag ccgtgtatgg tcccggcgat
    14521 aaagagttaa aaccgctgtt tgactggatg ctacgcggcc tgctgccacg acttggtgcg
    14581 ccagatacac aactctcttt tcttcacgtc accgattttg cgcaagcagt gggtcagtgg
    14641 ttaagcgccg aaactgtaca gacgcaaacc tatgaattat gcgatggcgt cgccggtggc
    14701 tatgactggc aacgcgtacg acagcttgcc gccaaagccc gttgtggttc cgtgcgaatg
    14761 gttgacattc ctctgccggt actcacaggc cttgcggata tcagtaccgc gttgagtcgc
    14821 ctggcaggta aagaacctat gctgacccgc tcgaaaattc gtgaattaac ccacgccgac
    14881 tggtcggcaa gtaataaccg tatttctgaa gatatcaatt ggtttcccgg gattagcctg
    14941 gaacacgcat tacgcaacgg gctattttga ttgtcttgtt ttaaaggtgt gttaacgttt
    15001 ctatggtaaa tcgtgaaata gtaatggatt acatcctttc ctgtttacag gatttggtcg
    15061 aaaacggtgt ggaaattaag cccgacagcg atctcgtcaa cgatcttggc ctggagtcga
    15121 tcaaagtgat ggatctctta atgatgctgg aagacagatt cgatatttcc attcctatta
    15181 atattctgct ggatgtcaaa acccctgcgc aattaatgga aactctactt ccatggctgg
    15241 agaataaata atggggctat acgataaata tgcgcgcctt gctggcgagc gtctgcaatt
    15301 tagtgacaac gggttaacac cgtttggcac ctgtattgat gaagtgtatt ccgccaccga
    15361 aggccggatc ggcaataaaa aagtgattct ggccgggacc aataattatc tcggtttaac
    15421 ctttaatcat gatgccatca gcgaaggtca ggccgcgtta gcggcccaag gcaccggcac
    15481 caccggttct cgtatggcaa acggcagtta tgcgccgcac ctggcgttag agaaagagat
    15541 tgccgagttt ttcaatctcc ctaccgctat cgtttttcaa ccggttatac cgctaattta
    15601 ggcgtcatca gcgcacttgc cgaccataac gccgtggtgt tactggatgc cgacagccac
    15661 gccagtattt atgacgcctg ttctctcggt ggcgcggaaa ttattcgttt ccgccataac
    15721 gatgctaaag atctggaacg gcgcatggtt cgcctcggtg aacgggcgaa agaggcgatt
    15781 atcatcgttg agggcatcta cagcatgctc ggtgacgttg cgccgctggc ggaaattgtt
    15841 gatatcaaac gtcgtctcgg tggttatttg attgtcgatg aagcgcattc gtttggcgtg
    15901 ttaggcgcaa cggggcgcgg actggctgaa gctgtcggcg ttgaagatga tgtggatatt
    15961 atcgttggta ctttcagtaa aagtctggcc tcgattggcg gttttgctgt cggttcggaa
    16021 gcgatggaag tgctgcgcta cggcagccgc ccgtatattt ttaccgcctc tccttctccg
    16081 tcctgcattg cgaccgtgcg ctcgtcgtta agaactattg ccacacaacc agagctgcgg
    16141 caaaagctga tggataacgc caaccatctg tatgacggtc tgcaaaaact gggttatgag
    16201 ttaagttcac atattagccc ggtagtgcca gtaataatcg gttcgaaaga ggaaggttta
    16261 cgcatctggc gcgagcttat ctcgccgggc gtgtatgtga atcttatcct gccgcctgcc
    16321 gcgcctgcag gcatcacgct gctgcgctgt agcgtaaatg ctgcccacag tcgtgagcaa
    16381 atcgatgcca tcattcaggc atttgctact ctgaagcagt aatgtgagcc cgccgtggtg
    16441 tttgcgccac ggcgggttta atgttgtctg tcataccaat acaccaggct caacgccacc
    16501 aacacaaacg ctaccggtaa accgagcgcg gtaacaggtg cgcttaacgc aaatagcaat
    16561 cccagattaa ccatgatttg atagctgatc caggtgagta agcccacgat gacgcctaca
    16621 gcgagacggc tacccatccc aggcgaacgc ggggcactaa aggtaaacgg cacagccagc
    16681 aaaatcatcg ccagggtcag gatgggttgc cccagttttt cccacagtgc aaggcggtat
    16741 tcgctgctgg gttgcccggt ttcctgcaaa taatggatgt aatgattaag ctgacggaca
    16801 gaaaaagtat tccccggcat tgataactct tcaagattca tactggtgaa ggccgattgc
    16861 caggcaagat tatctaatgt ttccagcgtt tctttgccat tcagccattt tttatgattc
    16921 acaccatgca ggacccacgt tttgtcgtct tcaatggtgg cactgcgtgc ataaatgtag
    16981 gattcaagcg agagatcgtc gcgataatga aatatctcca cgcccaccgg ctggccctgc
    17041 tcgttcagcg atttcaccgt cacaaattca ttaccacgcc tggcccacag catattgccg
    17101 gtaatgtcat cgtcctcccc caacgcggtg gcggtcgatt tgatttgcaa cgcctgttgc
    17161 tgcaatggcg acgccaccca ctcatctatc gcgcctaacg aaacagtcca caatatcccg
    17221 gcaaccagcg ccaccagtgc aatacggaag atagaaaacc ccgtgctgcg aatggcggta
    17281 agctcactgt ttttcgataa ctgccccagt ccaacaatac cacccaataa cgcgataaac
    17341 ggcgaaagtt cgaccagtgt gcgcggtaag gtcattagca ccaccagcac cgcctgagtc
    17401 cagcgataac cgcccgggct gacaccatcc agttcgttaa tcaggttgaa ggtggtaaaa
    17461 agcggcagca atagccctgc agcggcggca aaaccgagaa agagatgacg gattaaatag
    17521 cgactgaaaa cattcatcgg gaaaatttct gcagcaaaga gaagtcacgt gcgaccagta
    17581 tcagcaggcc cattagcatg agtccgggca ctaaccacaa accaggaatg aggggaatcg
    17641 cgccgttagc caccagtgta cggcagacgt cgccgccata aaaaatggca ataaacagca
    17701 acgtcagtgg caataacgtt gaaaatcgcc cttgccgcgg ttttacccga cttaatgaaa
    17761 tggccagcag cgccatcaac aatgcggtca ggccacggct ttgtcgccat tgcagttcgg
    17821 catgatcggc aggaaaggcg gagcgcgcca gctccgtgac cgatttcgct ttgcgtttga
    17881 cgttcgggct ttgatccagc ggattcagat gtaattgcag attacggtag atctgttcgt
    17941 tgtcgtcacg cccctgatga tcgagaagat aggcagtgcc gttgtgcaac ataacggtcg
    18001 gtttctcggg tgatgggtca accacatcaa ccgaaccggc gcggaaaata cgggttcgat
    18061 tggcagtaga agtataaatc agcgcgtcag tcaggcgatt attatcctga tcaaccgtct
    18121 gcgaaaggat cattcgtccg ttatcgttag tgttaaattt ctttgcccgc aactggcgaa
    18181 catccagctc cgactgcgac tgctgctcca gttgataaat ctgcgcataa gcccacggac
    18241 gaccatacat cgacagaagg gtgacaaaaa tacttagcgg tatcgccaga taaagaacgg
    18301 ctttgtacaa gcgtcccgga ctgccccccg ccgcagagat agcggtaatt tccgagtcgg
    18361 tatacatctg ccctagcgtc acgccaactg acacatacag accaacaggt aacaacatct
    18421 ccagtgcaat cagtactttg tagaaaacga tatccagcac aacatcaaga gctaatgtcc
    18481 catttgccgc ttcggtcaga taacgctgtg cggagtagct ggcgaaaata aagatcagaa
    18541 aaccgacgat aatcagcacc agacggcgcg taccacgcat gatgtagtgt tcaaccagct
    18601 tcatctcact aacgccctca atcctttgag ttttttattc attgatcagt ttctgcgcca
    18661 acgcaatgac atgatctacc tcttccggcg tcaggctgcc gtcctttgcc cactgaattt
    18721 gcccggcttt attcagcacc agaatggttg aactctgctc cggcaagcgc caggcaacgc
    18781 gtccctgtcc attgccgtca ataacgaatt gcgcccacgg atagcggcgt ttattttttt
    18841 cgattttgcc aacaacaaaa tagccagtac caaagatggc atcatcggtg ttcacaatgg
    18901 tggtgggctg aaaacgatcc tgcggaaagt ttgccgcttc aaccgcttta attaacagtg
    18961 agttcttttt cttcgccgac ttacgcccgg cgatatactg aataatgcgg acttttcccg
    19021 ctaattgtgt gctgttccac ttttggtagt gaatttcgtc gtcgctattc agaagcagct
    19081 cgccgccttc ctggatataa acaggggtaa ccgttttgcc agtgacgaaa ttgtgtgcct
    19141 gcacacccgg ggctaagagc atgataatca acgcaataat caaacgagat gacatcattg
    19201 ctccggctat agcggaaaac gcgaaatcag cagctctaag cgctgacaaa tatcgctatc
    19261 cagcgccaac ggtgcataat acgactggcg gttttccata tcatccgcca ccatgtcgtc
    19321 gtgtttatct cccatcaggc cattaatggc ggcgaggaaa cgggaaatct gtaacgcctc
    19381 tttattttca ggatcaagcg atcttaaggt gtcgtaataa gaaacctgct ggctgatgat
    19441 ttttcgcaca tcatgaaatt ccctgccggt tagtcgcggt ttggcaataa tctctttcag
    19501 ggtatgcatt tgcttttgcc gataggcaat aaaactgctt acgctttcta actggctatg
    19561 gcgtaatgaa tagttcacca acgaccagac cggagagctt aaataaatcc gtaacaggtt
    19621 gccgtaatac gacacaatat ttttcttacc gttacgaaag ccgtcctgaa aacgccccag
    19681 aaaaacggtc gttttgccga ataaaaatcc ggcctgatgc ttctttaaat acagccgttg
    19741 cgcaaaacgc aggtgcttgt agcgcgctcg catgtattta aattgtttgc gctcatcagc
    19801 agtcagctca tcgcctttgg cttgtttgcg aactaaacgc agtaattcct gacgatcaac
    19861 gccattgagc cagaattgca ggcataacgc gtaattatca tgaatgattt cggtggtaca
    19921 acttagtgca atagtcggcg gcaaactaac ctgtgcatca acgacatcat cttcttcaat
    19981 cgcaacaaat agacgctcgc tcgcctcaag cgggaaatat tttctcatca tcaggatttc
    20041 tctccggctg cgttataaag caaggaatgg cgctgaaaaa atgcctttaa ggttggaatt
    20101 aacgagagcg ccacgtccag acattgccca ccgaagtaag caaacgctgc tcccagcaca
    20161 ccaaacagat gacagaacat gaacagcaat acgatgtagc cgattgaggc gatggtttgc
    20221 gccacgagaa aagcgcgctg ttttcccgcc atcaatagca atgactcctg cgggaagccc
    20281 agcatcgaaa tcacaattgc gcccagcatc acctgaatca ggtcatacgc ttcgagatac
    20341 ttaacgccaa acaccagcga aatgagcggc ttgccgacga tcaacaccgc gagagccacc
    20401 agaataccga ttccgcccgc cagtaaaccg gacttcacac ctaacaacca cggtctggtg
    20461 gtgcgcggat ctaaacgcat cacctctggg taaaaacttt tacccagcaa acccgctggc
    20521 gttccggcgg cgtcgaagaa tgtcatggcg attttaaata acccggcggc agcgggtcct
    20581 aacacgatcc ccaccaacac tgtgctgcac gagttacgcg ccgaccagat ggagtgggca
    20641 atgttggttg accagacaaa actccacgcg cctttaatgt gtcgggcaga ctcaaacaga
    20701 ttcaatttga aggcgttatg gatatttcta cggcgtaatt cgcgcgcggc aaaccaccag
    20761 tacatggtgc cgccaaccag attcgacacg taccaggcaa taacaaaacc cgcaaaacca
    20821 aagtcaaaat accaggctgc gacgctcccc gctgcgcgca gaaaaggttt cgtcgcctgc
    20881 tgtacagcaa tcaaatcaaa gcgatctacc gcacgcagaa tgccggtcgg cgtggaggaa
    20941 gccattgaag gaatgagcgt gcaatacagc gccgccagcc aaaagctttg gtcatccaga
    21001 cctaatgaat gggaaaggaa tggcagtaag gcaatgccgc cgacaatcgc caccgcgccg
    21061 ctgacgatat ccagcgagaa ggaaaatgag acgacattgc gaaattgctg cggattattg
    21121 ttggtcaagg ctggcgttcc gtactgaacc accagctgcc atgtctgaaa cttaataaaa
    21181 tcgctgatcg acttggcgta cgattgcaca atcaccagta caccaaacat ggcgggcgtc
    21241 atccctttac cggcacacga gagcgccaac agccccagca aggcgctctc gacattactg
    21301 gagcctaacc aggcgctatt gcgaatcatg gtgcgaaacg cgccatctgc aaaccaatgt
    21361 ttgatgttaa aacccgccaa ttcagcctga ccttatcgtt gatagtaaaa aatatcccgc
    21421 cagccttaag ttaaacttcg gcggtcagaa acgatggcaa ccagagaaac cgccttctgt
    21481 acctgttcca gcacttcgct gtagggcgct ctggaatcaa tctcaagaat ttttgtgccg
    21541 ttatagccaa tcgtcgacat gacgccgatt ttgtcctgca gctcggcata ctcatggtca
    21601 ggcttgcggg agatggcagt atcaatatca atccccaaac gaatgattaa ttcaggacgg
    21661 tattgcgcca ttttttggta taaactccgt tcgcgctgcg ccagaaacat gctgattttc
    21721 ccggtcgcac gttcgacgcc gatccccggt ccgtcataat aaaagcccga aatttcagcc
    21781 tgcgggaagc gatcgctgac caccagaacg ccactttgcg ccagtcgctg aaccttgcgt
    21841 agattcgcca ttcttcgcag cgagaagcag tacataatca ccgccgccca tagcgccgga
    21901 gatttggttt tcatgctttg ggttttcgag gatttggccg ccaatcgccg ttccagccag
    21961 acgccaacca acggcaatcg tttgattttg tcgccgtctt cgccggagag cagccccaga
    22021 tagcggcgct cggtttgcca gtgttgttgc agcgatttca ccaggtcggt ggtcagcgtg
    22081 gatttaccgg tgccatcaca cccgaccacc gcaatcagcc ccggaatgta gttgggctgc
    22141 ggcgcggttg tactattaac agtttgtgtt tgtagtgcat ccattcgtta aatcatcccc
    22201 tatccatttt ggtgatcagg aaagcgcggc gtgaattgcg cgtagtgacg cttgcataat
    22261 ttcatcggct ggttgccgac catccagttc aaggatcttg gcgccattga atgtcagttg
    22321 cggcgtaacg gcgatttttt cctgtaacgc tgccagttgg tggtcaggtt tacgcgcaaa
    22381 cgcggtttgt tcatcaatgc caagacgaat caacaatacg ggtaaataag acgccatcca
    22441 ttggtacagc ttcaactcgc gctgccttaa catttttatc caaccgttac cgcccgtggt
    22501 ttttgccaat tgcgggccat caaagcgaaa acccggcact tcaacctgcg ggtagcggtc
    22561 ggtgatgagc agatagcctt gctggctttt acacaacatt ttgcgaaact tgtacgcccg
    22621 ccagcaggaa agcagataga taaccagcgc agtaatattg ccaggcggag ttaagggctt
    22681 ttcgtgtaca tgtgccgctt tacttcgcag ataacaccca caaggtgcgc caataacagg
    22741 gagctgtgaa atccattcgc caattcgccc ggacgattgc ccgagataaa tgtgttctgt
    22801 tggcattctt gctgccagtt catttaccag gcttgccgtg agggtcgatt tacctgaacc
    22861 atcacatcct acaatggcaa taacacgcac tggcgtggaa ttaattattg acatattacg
    22921 ttgattcacg aaaaacccgg cagtaaataa tgtattgaat attacgtggt ccgtgctcag
    22981 aatatccgtt caaccttatt gtgacaatgg ctaattgtga cattgacctg aattacgctt
    23041 tcataaaaac atattaacca aataaatatt tttaatggat atttaaatta aaggatatat
    23101 tcatgcagtc aataacacct ccattaattg ccgttattgg tagcgatggt tcaggcaagt
    23161 caacggtgtg tgaacatctt attaccgttg tcgaaaaata tggtgctgcc gaaagagttc
    23221 atttaggaaa acaggccgga aatgtcggtc gtgcagtgac aaaattacct ttgatgggaa
    23281 aatccttaca taaaacaatt gaacgaaatc aggtgaaaac agcaaaaaaa ttgcctggac
    23341 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
    23401 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
    23461 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
    23521 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
    23581 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
    23641 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
    23701 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
    23761 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
    23821 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
    23881 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
    23941 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
    24001 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
    24061 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
    24121 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
    24181 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
    24241 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatcg
    24301 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
    24361 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
    24421 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
    24481 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
    24541 gtaacggcat cgcgggtacg ggtaatttca tagccggacg cattcatatt gacgacgaag
    24601 ccagcagggg caatccccac cagtaccagc attaccaggc cgatcccttt ctgcccgtcg
    24661 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
    24721 aatggcggtt tacgtttgcc ctttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
    24781 tcacgctttt tcgtcccact ccagaaacgg cgcagcagga aaatcagacc acccgcaatg
    24841 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggttac ttcacgcagg
    24901 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
    24961 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac caaagaacca cgttcccagg
    25021 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaaaccgtg agttgacccc
    25081 atattcagca acaaatcgat tggtaacata tggacaatgg cataggcaac gctaagtccg
    25141 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggctgc
    25201 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
    25261 gcttcgtaga acaacacaaa tgccagagca agtaataaca aaagccccgt atatatatca
    25321 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
    25381 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
    25441 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
    25501 aacacaggta ttccactggt taatatattc acatatgaaa tgaataaata ttggaatata
    25561 taaatattga atattttgat taatccccgt actaattatt cttcataatc agtacggggt
    25621 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
    25681 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc aacggtgaag
    25741 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
    25801 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
    25861 aggtcgatat tgctaccaca gcgaccggcg atcggtttca ctgcgtaacc tgttttcacc
    25921 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
    25981 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagacc
    26041 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
    26101 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
    26161 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
    26221 ccggcagcat cccagcccag ttcatccagc ccgcgcaaga tacgcgtttc aaagcccgcc
    26281 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
    26341 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccag ttcgttaatc
    26401 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
    26461 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtattcgta aaccttcagg
    26521 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
    26581 caggagagac gcaaacgtgg ccagaggatt ttcggaatgt cgaacagcgc cagcaggttg
    26641 tcatctttca gcaccttgtc ggttgcgtga agatacatca gatgcagctc gttggtggct
    26701 ttaattagct cctgctcggc actctcggta atggtgtagt aatgatacgg atcctgattg
    26761 atcacctgac cgttggcctg cacataggcg ttttgtagcg gatctttttc atccagccat
    26821 ttaccgtcaa actggccttt gttttccaga cgcgctccac tgattttcag cagctcgcct
    26881 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
    26941 atggtggtgt catcaaaagt gtctttaagg gtatagcagc cgttttcgac caccatctcc
    27001 agctcgcgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
    27061 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
    27121 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
    27181 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
    27241 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
    27301 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
    27361 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
    27421 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
    27481 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
    27541 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
    27601 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
    27661 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
    27721 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
    27781 cgttttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacatcca ttgcaacttt
    27841 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctactggcgc
    27901 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
    27961 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
    28021 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
    28081 aatttggcta cttcctgccg caggatctgg caaaacggac tgaaacgttg aactggctgt
    28141 tctggttaca gggtgccgcg ccgttcctcg gcggtggttt tggtcacttt taccattacg
    28201 cgccggtgaa aattgagtac gccatcaacc gctttaccat ggaagccaag cgcctgctcg
    28261 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
    28321 cggatatggc gatttggccg tggtttggca acgtggtgct cggcggcgtg tatgatgccg
    28381 cagagtttct ggatgcggga agttataaac atgtgcaacg ctgggcgaaa gaagtgggcg
    28441 aacgtccggc agtgaaacgt gggcgtattg ttaaccgcac caacgggccg ctgaacgagc
    28501 agttgcatga gcgccatgac gccagtgatt ttgagacaaa tacagaagat aagcgtcagg
    28561 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
    28621 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacaaaag
    28681 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
    28741 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
    28801 ccagcagatc ggcagggtta ccttcgcaaa tcagggcgat attcacgtcg cgcttgatac
    28861 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
    28921 gaacgccaat acacattgtt aactccggtt atttcgattg ccggacaaac gcgaaaaaga
    28981 acctttagcg ggtgttccct ctcaccctaa ccctctcccc ggaggggcga ggggaccgtc
    29041 cgtgcgcttt ttaggcgttg tcctcagtct tattcaactt caatactttt gacgatcagc
    29101 gaatcgccgg tatcgacacg caaccgctcg ccgtgacaga gcggacactg cgcatcgtgc
    29161 tgatgaatct ccaccacctg gctgcaatcc cagcaccagg cctgggcggg tttatagacg
    29221 atatgtaaat cgcacccttg cgccaccgtt ccgtggcaga caatttcaaa actaaaacgg
    29281 acggcgctct cctcaacgca ggagagcgcg ccaatttcca gccacacggc ggtgacgcgc
    29341 ttaacatcgt gctgctccgc ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac
    29401 aactcatgca ttttcgctcc tcctgcggcc aaacagcagg gcgcgacgcc ctgcgtgtgg
    29461 tacatccgga ttggtgactg gcaacgaaag gatcattcgt gcgcagtcat ccgtcaggcg
    29521 ttggccttct tcaatcgaca tgctgtgcga aagcggcgac atcagcgaac aggagaggta
    29581 ttgcgaaaca ccgtccagtt cgccaacggt aaaggtcatg gtgccatacg gcagttgcag
    29641 accaattttt tcactcactt tgcgcagcgg ccagagttga tccgggccgg ggaagataac
    29701 tgcactcagc atccacgggg tgatcacaca ccccgtccac tgaccttcga acagcgtaaa
    29761 atcagaaaca tacaccggca ttgaaggatg cagaaaagag agatcgtgca tcgaacgccg
    29821 ggcaatttct tcaaacgctg cctgtacttg cgccttcggg gaggtctgga aacctgctat
    29881 ctcttcagtc atgaatcgcc tcccgtggga tggcttccac gcctgattcg cgcagcgcag
    29941 ccagaacctg ctcaagcgca ggttcaatca ttgcttcaac cgtcggcgtt aagccgatgt
    30001 gtggctccag cgattccggg atcacgccga ccagggtcag ctttttcgga aactcaccgg
    30061 tgaagcgcag ggccgacaat acgtcggcca ggccaagctg atgcggagag attttgttgg
    30121 taaacaacgc cggaacttct tcatcccgca ggatcatcat cgttcccgga gtgttctttt
    30181 tcgacacaat ggcatccgca ataatcagat gatcgcgatt tgccatgtcg ccaagcagct
    30241 ccattcccgc cgtgccgcca tcgaggatct caacataatc cggcagaatg tatcgttgct
    30301 ctaacgcttc gacaatccgc acgccgatgg cttcatcggt cagcaaaata ttgccgaccc
    30361 ctaagactaa aatacgcatt acagaacctt cactgaaacc acttcgttgc cgtcggcatc
    30421 cactacgtgt accgcacagg ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc
    30481 cagcggttta ttcggatcgg caaccggtgt acccaccagc gactgctcgt aaggaccgac
    30541 gtcatcattg aagttacgcg gaccagagtt ccaggttgat ggaacaaccg cctggtagtt
    30601 gctgatgata ccgtctttaa taaccatcca gtgagagagc ataccgcgcg gcgcttcgag
    30661 gaagccaaca cctttgaact cacccgttgc cggaatgttc ggcttcacaa aggtggtgtg
    30721 atcgcctttg ccgatattgg tgatcagtgc actgtattgg ttttgcagga tatcctgcaa
    30781 ttcacagcag tgaacggtac gaccaataat acggcccagc gtggagtgca gctgtgccac
    30841 ttccagcgtg ttgccagtca gtttctgata aatcgcaacg atttcattca gtttgttctg
    30901 ggtagattcg cgacctgccg ccagtttcac cagcatatta gccagcggac ccacttctac
    30961 cgttttgccg tagaaagtcg gtgatttcac ccaggaatat ttcccgtcgt cagaccaacc
    31021 atcataagcc ggaatggtgg taccttccca cggcgcctgt ggcgcttcgt ctttatacca
    31081 ggagtgcttc gcgctttcct gaatgccttt gatcaggtat tcatcggaat gagaagtgat
    31141 cggacgatac gaggacagat ccgcattctc aatgtagcct cccgggaaca ggaagctgcc
    31201 gtttttgctg tcggtcggga attccggcac gctcaggtag ttcaccgcac ctttaccgcg
    31261 cgtcagccat tccgggtaga acgcggcgat aaccgcggta tcaaccttat aaacctgctc
    31321 aacaaagtcg ctcagtttgt cgatgaaaga cttgatgtac atcaggcgct caaggttcaa
    31381 cacgcccaac ccgtcgaggt tgattgggtt cgcgacacca cccactgcca ggttctgaat
    31441 gtgcggcgtt ttaccgccca gcagcgccac gacgcggtta gcgtcacgct ggcactccag
    31501 cgcctgcagg tagtgcgcta ccgcaatcag gttcacttcc ggcggcagtt tcatcgccgg
    31561 gtgaccccag tagccattgg cgaaaatacc caactgaccg ctggcaacca gatctttgat
    31621 cttgttctga actttagtga actcttccgg actgttcaga tgccaggtcg aaacgccttt
    31681 cagcatttcg gaggctttgg ttgggtcagc ttgcagtgca gaagtgatgt ccacccagtc
    31741 cagcgccgaa agctgataga aatgaacgat atggtcatgc gtggtgtgcg cagccagaat
    31801 gatgttacgg atgtattgcg cgttaaccgg aacgtcgata ttcagcgcac tttctgccgc
    31861 acgaacggaa gacagcgcgt gagtggtagt acatacgcca cagatacgtt gcacaatcat
    31921 ccatgcatcg cgcggatcgc ggtttttcac gatctcttcc atgccgcgcc acatggtacc
    31981 ggaagcccat gctttcgaaa cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc
    32041 ctcaatacgg gttaccggat caatagtaat tctctggctc atgctttgct cgcctcatga
    32101 cgattatgat cgttttgttt taaaggagga agtatcggca gtagacgaat gagtacgatg
    32161 taagcgcaaa tctcaatagc cacaaaacca atagaaatca acagttcttc ccaggtcggg
    32221 aagtaggcgt aaccgccgcc cgggttgaat gccaccaacg aataggtcag acgccaggtt
    32281 gcacaaccta acagtgcgct cagtgctgac aggaacagca tgcgggaatc attacgcagc
    32341 ttcgccacac gcagaacgac cagcgggaag agcatcagca ggacttcaat ccagaacatc
    32401 acggagtaga agtcaccggc aaacgctaac gacagcttgt cgcgatagat aagctcgcca
    32461 aagcgcagcc cgatgaaaat cgccagcaac acactgatgg tgttggtcag cttgacgaac
    32521 aggctctttt catccggacc gttgccacgc agacccgcct gcaccagtga accttcaaag
    32581 atgacaatcg agaagcccat gatgaacgcc gtcagcagcg agaacagcgg caacatttca
    32641 tagctctgcc agagcggatg caccttgtag cccgccgaga tcatcagcga ccccattgaa
    32701 gactggtgca tggtcggcag cagcgcaccg agcgcgatga tgaagaacat caccttgttc
    32761 agacgttgta gcgacacctt ccagcccaga cgttcaaaca gtgccggagc aaactccaga
    32821 gccatcacgc cgatgtagat ggtcatacag accgccgtct cgaacagtac cgagttcacg
    32881 ttgaagtgac ccggaatgta gaagtacggc aggttccagt agcgacccac gtcgatagtg
    32941 atcgacaagc cacccagtga gtaaccaaac agactcgcca acagcgccgg acgcaccagc
    33001 ggatggtatt gcccacggtt aaagacgtat accgcccacg ccagcgccca gccgccacag
    33061 gcaaagccgg tgccaatcag caggtcaaac gcgatccaca cgccccacgg gaagccgccg
    33121 ttcaggtcag agacagagcc cagaccgaac accagacgct tcacaatcag gagcatacag
    33181 atgacgatta acggtccaaa aatcatgacc ggtttactga tgattttgcc gcccagcggt
    33241 tgtggatcat gactcatgat cgtctcctcc gtcgtgatgg tcgtttttgg tgttgcgacg
    33301 aaccagcacg gttaagcccg ccagcacagc cagtggtagc atcatgcctt tatacagggt
    33361 gtgttgaata ttttcggaac gcgcaccggt agaaagatcg tccagtttcg gcaggtcgag
    33421 attttcataa ggcacacccg tcagtaccag aacctgagta ccgccgccct ctttctcgcc
    33481 gtacagatgc ggataatatt tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt
    33541 ctgacgcgga tagtggtatt cgctgccagg cttcagcgcc agacgttttt tcgcctccgc
    33601 catcagctct tcacgcgtac cgaaaatcac cgcgcccgcc gggcacactt ctacgcagcc
    33661 aggcagaccg cctttatcga gacgttccac acctttctgg ttgcacagct cgcacttatg
    33721 cagcgcacca aacgggttgt tgtagtcgta cttcggcacg ttgtacggac aggcgaccat
    33781 gcagtaacgg caaccggtgc acacgtcttt gtcgtaatgg acaatgccgg ttttcggatc
    33841 ttttttcagt gcagagaccg ggcacacaga gacacagttc ggatcgacgc agtgcataca
    33901 ctgtttctta atgtacgcgt agccgttctc ctcctggtct ttgttgaccc ctgtgccgct
    33961 ggtccacacc tgaatgatgt tattggtata cggcgacagt ttgtcgttgt tcgaccaggt
    34021 ctgttcccct tgcgggttac gttcagggaa gttgatatcc tgacacttgg tgacgcaagc
    34081 ctggcagcct acgcacaagg tcgagtcgta caacatcccc agcgatcccg gaattggcgg
    34141 gcggttttca gcagccgcat gactgacaga cggcagcgcg cccgtcagca atgccccgca
    34201 ggaggctgct ttaataaaat tacgtctgtt cacggttatt ctccccgtga gtcagcgtta
    34261 tctttctttt gctgacgacc cagttcacgc accgccatca cgctgacacc ggcaaccagc
    34321 ccaaccacac cgccgagcaa accaatagcg cctgcagaga cgttgccgcc ctctttagcg
    34381 ttaacatccg gtttctgtga acgcggagtc tgattttcga cgttggcaag ctgatggatg
    34441 cctttatgga agccgatacc tttttcgtta cagccatagc acgggtgacc aatcgccacc
    34501 ggccacacac cgccaacatc gcagaattgc agcgttgagc agttgccgta agtttctggc
    34561 cctttacagc cgaggtggta caggcaccag ccttcgcggt ggccttcatc accgaactct
    34621 ttggcaaaac gaccagcatc gaagtgcggg cgacgttcgc agtgttcgtg aatcagacgg
    34681 ccataggcga aggtcggacg gtttttgtca tccagtttcg gcggtttgcc gtaagtgatg
    34741 atgtgcgcaa cggtcgcgag gaagttgtgc gggttcggcg ggcagcccgg aatattgata
    34801 acggttttgc ctggcagaac ttcttgcaag ctgactgcgc cagttgggtt aactccagct
    34861 gcggcaacac cgccccacgc ggagcaggaa ccaatagcga taatggctgc tgagccttcc
    34921 gccgctttgc ggatgtgatc cacaatcggc tcaccggcaa ccatgcaata aataccgtta
    34981 tcttttaatg ggatggaacc atccaccact aacacatact gccctttgta cttctcgaga
    35041 gcgttatgtt tgttctcttc gacctgatga ccgaaggcgg cggaaagcac ttcgtgatat
    35101 tccagagaga tagtctccag cacgaggttt tctaccgttg gatgcgttgc acgaagcaga
    35161 gattccgtac aaccggtgca ctcctgcgcg ccaatccaga taactggcgg acgctgcggg
    35221 ttagtaaccg attcggccat ctctgcagcg gctttgctac ttaaccccat ggtggcggct
    35281 aatgctgcac aaagcttcat gaaatcacga cggttaatgc cgtgagaatg gatgagggtg
    35341 ttatctccag tcatttatag ttattccgtt gcgaggacct ggcatatatt ttgcctcaat
    35401 cgcaaaatca ataatgcgat cgatgcgcca tttaccacac atttattatg gttatcgata
    35461 tcatgatact gcggcggggc gctaaaacga agggaagaag cattagtgta attaattaaa
    35521 gcaagataat acgtatgttt gatcaatttt cgaggcaata accggccata aaaaagcgca
    35581 acgtagaaca ggaattattc tcacgaaaga aatccattac catctcttta tccagtccat
    35641 agcggcgcgt cagaataccc gcttcccagg cgctaagctg gcgatctccg gtcttttcgt
    35701 acatccggtg tgagtagcct aatacaaaac cgcgtttata atcggcacag aattttgtca
    35761 cattaaccgc actgtcggca tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat
    35821 ggttattcat cgttcacctc attcgttata tatcgccata tatagcgata ttttaggcaa
    35881 agaacgactt ttgcgcacgc tattttttca tacttgttta agacgtaatc ccgtaagaga
    35941 taaacctatc aggagagata tttatcgaac catttcagtg tcctttgcca ggcaagatcg
    36001 gcggcagatt tgtcataacg gggcgtggaa tcattatgga atccgtgatt aacccccgga
    36061 tagatataag cctcataaac cttattattg tctttcaacg ccgcctcgta aggaggccag
    36121 ccctcgttga ttcgggtgtc cagttccgcg aagtggagta gtaaaggcgc ttcaatcttc
    36181 gccacatcgg cagtgggtgc ctgacgacca taaaacggca ccgcgcaggc cagttccgga
    36241 tacgcgacag ccgccgcgtt cgatacgcca ccgccatagc aaaatccggt aatacccact
    36301 ttacctgtcg cttgcggata gcgttgcata aactcaattg cggcaaagaa atcattcatc
    36361 agtttggttg gatcaacctg ctgttgcagc tcacgacctt tatcatcatt tcccggataa
    36421 cctccaacgg aacttaatcc gtcaggtgcc agggcgatat accccgcctt cgccactcgc
    36481 cgtgccacat cttcgatata cggattcagt ccacgattct catgcaccac ccccacggct
    36541 ggcgttttgc cgctcatttt tgcgggtttc accagataac cccgtacctc gccgtgacca
    36601 tttggcgaag gatacgtgat gtactcagca acaatctccg ggtcggtaaa ctctacctga
    36661 gtcgccagcg cataatttgg cttgagcaaa tcaaacaacg ctaatgccgt catcccgccc
    36721 accgcatact tcgccgcaag gttgaggaac tcacgtttcg aaattttccc gtgaacgtaa
    36781 tagtcgtagt aatccaacaa ctcttgtggg aaatctttgg cggttaaacg cggcatcgtt
    36841 gcattcctca actggtgttt ttttaagcaa agcataagcg cgtgtttttg cccagttttt
    36901 cgtcactctg tgagccagac tacgggatac gcgctggcga atcgctaaac tagaaacatt
    36961 gtttcgaaat tgaacggagg aaaggagagg tcatggtctg gttagcgaat cccgaacgtt
    37021 acgggcagat gcagtaccgc tattgcggaa aaagtggttt acgcctgccc gcattatcgc
    37081 tcggtttatg gcacaatttc ggtcacgtta acgcgctgga atcacagcgt gcaatcctgc
    37141 gtaaagcgtt tgatttaggc attacgcact ttgatttagc caacaattac gggccgcctc
    37201 caggaagcgc agaagagaac tttggtcgcc tgctgcgcga ggattttgct gcttatcgcg
    37261 atgaactgat tatctctacc aaggctggct acgatatgtg gcccggcccg tacggctctg
    37321 gcggttcacg taaatacctg ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg
    37381 agtatgtcga tatcttttac tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg
    37441 cctctgcgct ggctcatgcg gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt
    37501 actcgccaga gcggacgcaa aaaatggtcg agttgctgca cgagtggaaa attccgcttt
    37561 taattcatca accttcgtac aatttactga accgctgggt ggataaaagc ggcctgctgg
    37621 ataccctgca aaataacggc gtgggctgta ttgcctttac tcctctggct cagggattgc
    37681 tgaccggaaa atatctcaac ggcattccgc aagattcacg gatgcatcgt gaagggaata
    37741 aagttcgtgg tctgacgccg aaaatgctta ccgaagccaa cctcaacagc ctgcgcttat
    37801 tgaatgaaat ggcacagcag cgtggacaat caatggcgca aatggcgtta agctggttgc
    37861 tgaaagatga tcgcgtgacg tcggtattga ttggtgccag ccgcgcggag caactagagg
    37921 agaacgtgca ggcgctgaat aatctgacat ttagcaccga ggagctggcg cagattgatc
    37981 agcatatcgc cgatggcgag ctgaatctgt ggcaggcgtc gtccgataaa tgacctgtta
    38041 ataacgggcg cgcgaaccgc gcccgaatca gatcagtgat tatgacgagt cagtcggtca
    38101 agatagccca tcacaaatgc agaaagcaca aacgtcagat ggataatgac gtaccacatc
    38161 agtttgttat ccgggacatt tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa
    38221 attgccacaa tcgacgctgc tactttgttt ttcagcgacg ttgcgtccat tttccccagc
    38281 cacttcagct tctctttgtt ctcggagata tccagctgtg agacgaaatt ctcataaccg
    38341 gaaaacatca ccatcaccag caaaccgcca accagcgtca tatccaccag cgacagcaac
    38401 acgaggatca aatctgattc cgccatcgag aagatattcg gcagtacgtg aataatctcc
    38461 tggaagaact tcagcgccag ggcaactaac gccagcgaaa ggccaaagta cacgggggca
    38521 agcagccagc gagaagcata cattgcattt tcaagaaaac gttccataca ttcctgtcgt
    38581 gtagttaaaa cgggcaacag tatatcgcaa ttgagtaaac agcacgcaac agcaaatcaa
    38641 catatacgtt aaacctttgt cgcataattc tgaggtttag ggatcttcct ggttccctta
    38701 tcaacgcatc gagagcgtct acccttaaag gacgttgaac ctgaagggag aaaaacgatg
    38761 tctcatttaa aagacccgac cacgcagtat tacactggtg aatatcccaa acagaaacaa
    38821 ccgacgccag gcatccaggc gaagatgaca ccgataccgg attgcggcga gaaaacctat
    38881 gttggtagcg gtcgcctgaa agatcgtaaa gcactggtga cagggggcga ttccggaata
    38941 ggtcgcgctg ccgccatcgc ttacgcgcgt gaaggggctg acgtggcgat cagttatctt
    39001 cccgtggaag aagaagacgc tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa
    39061 gccgttctgc tgccaggcga tttaagcgat gagaaattcg cccgttcgct ggttcacgaa
    39121 gcgcacaagg cgttaggcgg gctggatatt atggcgctgg tcgccgggaa acaggttgcc
    39181 attccggata ttgcagacct caccagcgaa cagtttcaaa agacctttgc cattaacgtt
    39241 ttcgcgctgt tctggctaac ccaggaagcg atccccctgc taccgaaagg tgcaagtgtc
    39301 atcaccactt cgtcaatcca ggcataccag ccaagtccgc atttactgga ctatgcggct
    39361 acgaaggcgg cgattctgaa ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt
    39421 attcgggtga atattgtcgc gccaggccct atctggacgg cactgcaaat ttccggcgga
    39481 caaacgcagg ataagatccc gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa
    39541 ccggcggaac tggcccctgt atatgtttat ctggcaagtc aggagtcgag ctacgtcacc
    39601 gcagaagtgc acggcgtgtg cggcggcgag catttaggtt aaaaaaatgc ccggttgtga
    39661 aaagcaaccg ggcatcattg tgaattcact cttacctgat gtggcgaaaa cgccttatcc
    39721 gcctacaaac gcatgaagat tcaacatgtt gcaatagatt ttgtaggcct gataagcgta
    39781 gcgcatcagg cattgagcac ctaatgcctg atgcgtcgtg aacgccttat ccggcctaca
    39841 aacgcacgaa gattcaacat gttgcaatag attttgtagg cctgatgagc gtagcgcatc
    39901 aggcaattct actttacttc gctttggcgg tttcttcgcc gaccagacct atcttcaggt
    39961 aacccgcctg atgcagcgta tccattacct tcatcaacgt ctcgtaatcg acggttttgt
    40021 ccgctcggaa gaagatggtg gtgtctttct tgccttcggt taacgcattc aacgccgtaa
    40081 tcattgtttc atcggtgacc ggatcgttac cgataaacat cgagttgtct gccttcaccg
    40141 acagataaac cggtttttcc gggcgcggct gcggcgtgct ggtagaagca ggcaagttca
    40201 ccttcacatc taccgtcgct aacggtgccg ccaccataaa gataatcagc agaaccaaca
    40261 tcacgtcgat aaacggcgtc acgttgatat catgcatttc gccgttatcg tcgaggtttt
    40321 cgttaagatg cattgccatc ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg
    40381 gatgcgcagc ggcgctggct tccagatcca ggtcacggct ttgcagcaac aatacctgcg
    40441 ctgcaacatc acccagcatc gctttaaagc cgccaatctg gcgtgcaaat acgttataga
    40501 taacgaccgc aggaatcgct gccacgaggc cgattgccgt tgctaacaga gcttctgcga
    40561 tacccggcgc aacgactgcc aggttagtgg tctgcgtttg cgcgatacca ataaagctgt
    40621 tcatgatgcc ccagaccgta ccaaacagac caacgaacgg agaaatcgcg ccgatggttg
    40681 ccagatagcc gttaccgcgg cccatttgac gacccactgc ggcgacccga cgctccagac
    40741 ggaagctggt acgttcttta ataccttcgt tatcgtcgct gccttctgac agttccagct
    40801 cattctgcgc ttcattgagc aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga
    40861 tatcgttggc ctggtttaag gaacgtgctt cagccagcag ttgctgctcg cgcttaaggc
    40921 gacgcttctg attgaagaac tctacgctct tactgaagaa gattgcccag gtgactacag
    40981 aggccaaaat aagcccaatc atcacgcact taacgacaat atcggcgtac tgatacatac
    41041 cccagacgga aaggtccgtc tgcattaaat tattacccac tgtgtatctc caggacgcaa
    41101 gtcacaaaat ctgcgcataa taatatcaaa acgacgtcga attgatagtc gttctcatta
    41161 ctatttgcat actgccgtac ctttgctttc ttttccttgc gtttacgcag taaaaaagtc
    41221 accagcacgc catttgcgaa aattttctgc tttatgccaa ttcttcagga tgcgcccgcg
    41281 aatattcatg ctagtttaga catccagacg tataaaaaca ggaatcccga catggcggac
    41341 aaaaagcttg atactcaact ggtgaatgca ggacgcagca aaaaatacac tcttggcgcg
    41401 gtaaatagcg tgattcagcg cgcttcttcg ctggtatttg agagtatgga agccaaaaag
    41461 cacgcgacgc gcaatcgcgc caatggcgag ttgttctatg gacggcgcgg aacgttaacc
    41521 catttttcct tacaacaagc gatgtgtgaa ctggaaggtg gcgcaggctg cgcgctattt
    41581 ccctgcgggg cggcagcggt tgctaattcg attcttgctt ttgtcgaaca gggcgatcat
    41641 gtgttgatga ccaacaccgc ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc
    41701 aaactgggcg taacgacatc gtggtttgat ccgctgattg gtgccgatat cgttaagcat
    41761 ctgcagccaa acactaaaat cgtgtttctg gaatcgccgg gttccatcac tatggaagtc
    41821 cacgacgttc cggcgattgt tgccgccgtg cgcagtgtgg tgccggatgc catcattatg
    41881 atcgacaaca cctgggcagc cggtgtgctg tttaaggcgc tggattttgg catcgatgtt
    41941 tctattcagg ccgccaccaa atatctggtt gggcattcag atgcgatgat tggcactgcc
    42001 gtgtgcaatg cccgttgctg ggagcagcta cgggaaaatg cctatctgat gggccagatg
    42061 gtcgatgccg ataccgccta tataaccagc cgtggcctgc gcacattagg tgtgcgtttg
    42121 cgtcaacatc atgaaagcag tctgaaagtg gccgaatggc tggcagaaca tccgcaagtt
    42181 gcgcgagtta accaccctgc cctgcctggc agtaaaggac acgaattctg gaaacgagac
    42241 tttacaggca gcagcgggct attttccttt gtgcttaaga aaaaactcaa tgatgaagag
    42301 ctggcgaact atctggataa cttcagttta ttcagcatgg cctactcgtg gggcgggtat
    42361 gaatcgttga tcctggcaaa tcaaccagaa catatcgccg ccattcgccc acaaggcgag
    42421 atcgatttta gcgggacctt gattcgcctg catattggtc tggaagatgt cgacgatctg
    42481 attgccgatc tggacgccgg ttttgcacgg attgtataac attgccactt ttggacaatt
    42541 ttgcagacat tttattgtga aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg
    42601 acgattcagg agtacaatag gcagataaag gcttaaacgc tgttccacag gaaagtccat
    42661 ggctgttatt caagatatca tcgctgcgct ctggcaacac gactttgccg cgctggcgga
    42721 tcctcatatt gttagcgttg tttactttgt catgtttgcc acgctgtttt tagaaaacgg
    42781 cctgctgccc gcctcatttt tgccaggcga cagcttgttg atactggcag gcgcattgat
    42841 tgcccaaggg gttatggatt ttctgcctac gattgcgatt ctgaccgccg cagcaagtct
    42901 gggctgctgg ctaagttata ttcaggggag ctggttaggg aataccaaaa cggtgaaagg
    42961 ctggctggca cagcttcctg ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca
    43021 cggtctgctg gcgctgctgg ctggacgttt tcttgcattt gtccgtacgc tgctgccaac
    43081 catggcggga atttccggtc tgccaaaccg ccgcttccag tttttcaact ggttaagtgg
    43141 attgctgtgg gtcagcgtgg taaccagttt tggctatgcc ttaagtatga ttccgttcgt
    43201 taaacgccat gaagatcagg taatgacgtt cctgatgatc ctgccaattg ccttgttaac
    43261 cgctggcttg ttaggcacgc tgtttgtggt gattaaaaaa aaatactgta acgcctgacg
    43321 attttccccg ttcccggctg ctgtaccggg aacgtattta attcccctgc atcgcccgca
    43381 ttcttgccgc atcttccccc ggcgtcacac cgaagtaacg tttaaactca cggctaaatt
    43441 gcgatgcgct ttcatagccg acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga
    43501 tgatcatcat ccgcgcctta tgcagacggt aattcttcaa atactgcaac ggcgaggtgc
    43561 tggtgacaga cttaaaatta tggtggaacg ccgatacgct catgttggct tctgccgcca
    43621 gttgctcgac gctcaggttt tcggtgtatt tattctcaat tcgtttcagc acgcggctaa
    43681 tcagactgaa gtgagtctgg cgactgacca gcgccagtaa cgcgccgccg caaggtccgg
    43741 tcagcacgta gtacagaatt tcgcggatga tctgtttgcc gagaatacgc gcatccagtg
    43801 gtcgctccat cacgtcgagt aaccgctccg ccgcgcataa aatctcttct gataacgtgg
    43861 cggagttaat cccgctggct gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca
    43921 tcaacagttc ctgtaactgc aaaatatcga cattgagacg caaccctgcc agcggcacct
    43981 ctgacgtggc ataggtttcg cactcaaacg gcaacggcac cgtcagcagc aggtattcat
    44041 tggcatcata acgaaacacg cgttcattga tataaccgat tttatgcccg gaaaagagaa
    44101 ttatgatgcc aggctcgtac atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca
    44161 aacgcacatc gggcaacagt tctgacaaac tattttcttt atttttcagt ttattaactt
    44221 tatccgccag caagcgacaa atctcttcac gtttcatatc gcgtaatttc ttaggaataa
    44281 tgcggcaatt tgattgtgcg caattttgta gcatttctcc agcactctgg agaaataggc
    44341 aagacattgg cagaaatgag cattgagagc cagggcgctg gcgatcacaa tgaaaaacat
    44401 caggcagatc gttctctgcc ctcatattgg cccagcaaag ggagcaagta atgaacaact
    44461 ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg
    44521 aacaaattcc tcacgatgct cgcgtattga ttacctacgg tggcggcagc gtgaaaaaaa
    44581 ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg
    44641 gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac
    44701 agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc accaaattta
    44761 tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg caaacgggcg
    44821 gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt
    44881 cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag caggcgttcc
    44941 attctgcaca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc tacaccctgc
    45001 cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg gaacagtatg
    45061 ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt ttgctgacgc
    45121 taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg
    45181 tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cactggcgta ccgcaggact
    45241 gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat cacgcgcaaa
    45301 cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc
    45361 tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgacgat gagcgtattg
    45421 acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg acccacctct
    45481 ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg gaagagcacg
    45541 gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc cgtatatacg
    45601 aagccgcccg ctaagctttt tacgcctcaa actttcgttt tcgggcattt cgtccagact
    45661 taagttcaca acacctcacc ggagcctgct ccggtgagtt catataaagg aggaacgtat
    45721 ggctaatcca accgttatta agctacagga tggcaatgtc atgccccagc tgggactggg
    45781 cgtctggcaa gcaagtaatg aggaagtaat caccgccatt caaaaagcgt tagaagtggg
    45841 ttatcgctcg attgataccg ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct
    45901 gaaaaatgcc tcagtcaaca gagaagaact gttcatcacc actaagctgt ggaacgacga
    45961 ccacaagcgc ccccgcgaag ccctgctcga cagcctgaaa aaactccagc ttgattatat
    46021 cgacctctac ttaatgcact ggcccgttcc cgctatcgac cattatgtcg aagcatggaa
    46081 aggcatgatc gaattgcaaa aagagggatt aatcaaaagc atcggcgtgt gcaacttcca
    46141 gatccatcac ctgcaacgcc tgattgatga aactggcgtg acgcctgtga taaaccagat
    46201 cgaacttcat ccgctgatgc aacaacgcca gctacacgcc tggaacgcga cacacaaaat
    46261 ccagaccgaa tcctggagcc cattagcgca aggagggaaa ggcgttttcg atcagaaagt
    46321 cattcgcgat ctggcagata aatacggcaa aaccccggcg cagattgtta tccgctggca
    46381 tctggatagc ggcctggtgg tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa
    46441 ctttgatgtc tgggatttcc gtctcgacaa agacgaactc ggcaaaattg caaaactcga
    46501 tcagggcaag cgtctcggtc ccgatcctga ccagttcggc ggctaacgtg caaattctcc
    46561 cggtggcggt aatgttccgc taccggactt ttcagaaatc atttattccc ctcgcgtccc
    46621 gcccgttgtt actcttcctt gttcaggaat gccaaatata aggacatcat catgcagagc
    46681 cggaagctct taaaagaaca actcatctat atccgggata aacgcaacgg agaggtgaaa
    46741 aacagatgaa aataatactt ctgtttttag cagccctggc aagttttacc gtacacgcgc
    46801 agcctccttc acagaccgta gaacaaacag tccggcagat ttatcagaac tataaatcag
    46861 atgccagtac gccttatttc ggtgaaaccg gagagcgggc gataacttca gcgcgtattc
    46921 aacaggcgct taccctgaac gacaatctta cactaccggg caatattggc tggctggatt
    46981 atgatccggt ttgtgattgt caggattttg gcgatctggt gttagaaagc gtagcgataa
    47041 cccaaactga cgtcgatcat gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata
    47101 aagaaaagac cacacagaca ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgatg
    47161 atattgtcag caatcatggc agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc
    47221 tggccgcttt agcttcattg cagaaagaac agccagaatc ctttgttgct gaactctttg
    47281 aacatattgc tgattatagc tggccgtgga cgtgggtggt ttccgactct taccgccagg
    47341 cggttaatgc cttctataaa accaccttca agacggccaa taatcccgat gaagatatgc
    47401 aaatagaacg gcaatttatt tacgacaatc cgatctgttt tggcgaagag tcgctatttt
    47461 cacgcgttga tgaaattcga gtcctggaga aaaccgccga ttccgcccgc attcatgttc
    47521 gttttacgct gaccaatggc aacaatgaag agcaagagct ggttttacag cggcgcgaag
    47581 gtaagtggga aatcgctgat tttatccgcc cgaacagcgg cagcctactt aagcagattg
    47641 aggcaaaaac cgccgccaga ttaaagcaat gagctgaatt aaataacaat tagccggaac
    47701 aataaataaa agggaacact atatgaaaac gattttcacc gtgggagctg ttgttctggc
    47761 aacctgcttg ctcagtggct gcgtcaatga gcaaaaggtc aatcaactgg cgagcaatgt
    47821 acaaacatta aatgccaaaa tcgcccggct tgagcaggat atgaaagcac tacgcccaca
    47881 aatctatgct gccaaatccg aagctaacag agccaatacg cgtcttgatg cacaggacta
    47941 ttttgattgc ctgcgctgct tgcgtatgta cgcagaatga taaaaaaatc cccggcagca
    48001 tgtcagttgc cggggatttt ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac
    48061 cagcattcac cggacgagat tgcgtagacg acgctttttt tgccgtagca ggcgtctgac
    48121 gctgagtcgc catcggcgta tgtttagtca acgccggacg ggtattgcgg ttctggcgac
    48181 gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctgccaa
    48241 tcagatgctt tttgcccatc gcttccagcg cctgacggat taacggccag tttgccggat
    48301 cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga
    48361 cgccttcgct cttataacca atcttcgcca gcggtttttt cccggtgtaa tacatggtgg
    48421 ttgagttcgc cagcggcgat gggtagaagt tctgtacctg gtcgagacgg aaacgatgct
    48481 ttttcagcca cagcgccaaa ttcaccatat cttcatcacg cgtaccgggg tgcgcagaga
    48541 tgaaatacgg gatcagatac tgctctttac ctgcctgttt cgagtaggta tcgaacagct
    48601 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgacaac ggcccttctt
    48661 cggtatgttc cggagcaatc ttcagataac cgccgacgtg atgggtcgcc aactctttga
    48721 tatagcgcgg atcttccacg gcgatgtcat aacgtacgcc tgaggcgata aggatttttt
    48781 taatgccttt cagatcacgc gcacggcgat agaggttgat cgtcggttcg tggttagtgt
    48841 ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg gcgacaagtt tgttcagcgc
    48901 gtggcgattt gcagcgcaac atatacatgt tggcagttgg cccaccgaga tcggaaatca
    48961 cgcccgtaaa acctggaacg gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt
    49021 ccgaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa gaacagccgc
    49081 caaagcagcc gcgcataatg ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg
    49141 cattgccata ggccggatgt ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct
    49201 cttcggtaga aagcgggata gcaggcgggt tgatccacac atagcggtca ccgtgttttt
    49261 gcatcaatgc gcgggcgcag cctgggttgg tttcgtggtg cagaatgcgc gaagcatggg
    49321 cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaatacg taggtttttt
    49381 cccacggttt ctggcgcggt ggctgcacga ttacagcttt cgcttcctgc tttttcggcg
    49441 caacaggttt gttatccgca cacggcaaat cttcgccata cggatgcggg attggggcga
    49501 ttttccctgg ggtatcaaga cgggtggaat ccacaccgct ccagccaggc agcgcctctt
    49561 ttacgataat tgcggtatta cgcacatcgc ggatttcact aatcggctcg cccatcgcca
    49621 gacgatgcac tacttccacc agcggacgtt caccgttacc aaacatcagc atgtcggctt
    49681 tcgaatctac cagaacggaa cggcgcacgg tatcggacca gtaatcataa tgcgcggtac
    49741 ggcgcaggct agcctcaata ccgccgagga tcaccggcac atctttccac gcctctttac
    49801 aacgctgggt ataaaccagc gtggcgcgat ccggtcgctt acccgcgaca ttatctggcg
    49861 tgtaggcatc atcatgacgt aaacggcgat cggcggtata gcggttgatc atcgaatcca
    49921 tgttgccagc agtaacacca aaaaacagat tcggtttacc cagacgcata aagtcgtctt
    49981 tgctgctcca gtccggctgg gcgatgatcc cgacgcgaaa gccctgcgct tccagcatac
    50041 gaccgcaaat cgccatcccg aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa
    50101 tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgagacatc ggcaaaaacg
    50161 gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg cgatccggtt
    50221 ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa tcaaaggccg
    50281 gggattataa gccggaacga aagagaaatc gaaaggtatt ccatactcgc cctcctcggg
    50341 cgagtatgaa gattacggta ccggattgac caacagttgc ccaatcgagc cacggtccgc
    50401 catttccagc gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa
    50461 ataaacaagc agctccactt gtccgtcaac ccaaacggta tctttccagc ctctgtcttc
    50521 cgggaacggc atcgcgccat tcacgttacg gatctggaac attacgcctt caatatggaa
    50581 cgcttgcggc tcgtccgcgc gtaccgtcca gcgttcccac gttccttgct gcgcggtgac
    50641 atcaatacgg ttgacgtccc acagctgtcc attaataccc gggtcatcac ccagactgat
    50701 atcgcgactg cgaattggcg aaccagccat gatttcagtt ggcagcaagc gcatcggaag
    50761 actgtctgtg accagcggca gaaggccggt tgggcgcagc gttagcacca aggtagaaac
    50821 cagaatactg gatggctcaa agaagccgcg aatacgatca acaatgctcg ccgcttcacc
    50881 acaggtgatc gacacttcat cgccgttgct catatccacc agaatctcgc ggcgctcgcc
    50941 cggtgccagc gaaagttgct tcaccgatac aggagcaggc aggaatccct gatcgccaga
    51001 aatcacatgt aacgggcgac catcgctcat ctgtagttga tagcgacgag agttcgacgc
    51061 gttcagcagt cgcaagcgca cccagccacg cgagacttca acgtacgggc tttgtacacc
    51121 gttaaccagc agcgtatcac caacaaagcc gccgcttccc ggttcgttgt attctggcgt
    51181 accaaagtta tccagccgtt tatcctggat aatgaccgga aaatcatcca caccataatg
    51241 gttggggata ggcagcgact tgctgacttc atcttccacc agccacattc cggcaaggcc
    51301 gttatagacc tgctgagccg tgcggttggg agtattggcg tgataccaca gagtagctgc
    51361 gttctggcga ataggcagta cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg
    51421 accgcccatc agcgggcctg gtacctgtag cccggccacc gtcattgaga cattttctgt
    51481 caggcggttg ctgtaaataa gcttaacatc gtcgcccttc cagacgcgga tagtcggccc
    51541 caggtaacga ccattgattc cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg
    51601 cgcacgttgt acagtcataa acagcggttg cccacggcga gattcaagta gcggcggaac
    51661 gggtagcggt tgctgttgcc cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag
    51721 tgcaatcccc gatgcctgaa tgaactgacg ccgactgagt gacatatttg ctccatgtaa
    51781 aactggctaa agtaataccg tgcaggaaag tgttccccgc aaaaatacag attgccgact
    51841 taaacttttc cggcggcttc gcgttctgcg acttctttat cgagctcggc gattttttgt
    51901 tccattatcg aacgacaatg ggcagccagc tcacgaacct gatctttgcc atactgactg
    51961 acgtcaattg gcggcagcat ttcgacaatc accagaccgt tgtgcagtcg attaagatta
    52021 atcttattcg aagttgtaga gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc
    52081 gcgtgaaatg ctccagtctt gaacggtagc aggccgcgac cacggctgcg ggttccttcc
    52141 gggaacatcc agatggaaat acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg
    52201 ccgtgagctt tagtgcgatt gtttctgtcg atcaataagt tgccggttaa ccagtacaac
    52261 tgcccgaaga aggggatcca cagcaagctc tttttaccta ccgtcaccgt cggcggttgc
    52321 acaatgttcg atgctgtcac catgtcatag ttgttctggt ggttagcgat atagatagca
    52381 ttgccgtagt tttcagcatc tgcaggtttg cggcactcaa ctttcaggcc gaacagtggc
    52441 gcaagacggc caaacatatg cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa
    52501 aggcagtaaa tggagccgaa tacacagact aagatgctgt aaatcacggt aataataaga
    52561 cgaaagatat atagcataac accctctgaa ggtctgagag actggcattg tacgtcacct
    52621 gaaatcaggt tagggcattg ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa
    52681 cgcatgaata aacaacggtt tcaagggaat ttttatcgtt tccgggggtt gaatacgctg
    52741 ccggatgacg acttaacgtt tcatccggcg ttccttgcta gcgggaggaa acagcgccct
    52801 ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa
    52861 cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga
    52921 ctttctgtag ctcttccgga cgcagtttaa ttttgcgttt cccaacatga atggtcagcg
    52981 tgctttgcgg cggcagaacg tacagttgcg ccagaccatc ctcgccacgc gcggcttctg
    53041 ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc agatcactta
    53101 ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg gaagcatctt
    53161 caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa gccttacccg
    53221 cacggttacg cgctaccaga tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag
    53281 ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc gccccaggcg
    53341 gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc agcgtaatcg
    53401 ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg ttactcttac
    53461 ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc gcgtcgatat
    53521 catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg acaggttcag
    53581 acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac
    53641 gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc aggttattca
    53701 ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc agctcactct
    53761 gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc agttcgagga
    53821 tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt ttcggttcat
    53881 cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc agcaaacctt
    53941 ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg cggcgcacgg
    54001 tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc accgccggac
    54061 gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga tcggtggtag
    54121 cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc acaatcacca
    54181 gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc ggcagctttt
    54241 tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc tgatgcggta
    54301 atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata cgcactgaac
    54361 cacgtccgtt ctcgtagatt ttacgaatct cggcgcgcga agtgataatt tccgcttcag
    54421 tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtagtt ttcggctggt
    54481 cgattaatgc gattgccgcc tgagctactt cacgcaggtt atgcggtgga atatcggtcg
    54541 ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga cgggcaggta
    54601 gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc
    54661 ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg taacgcattg
    54721 ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca accagcggat
    54781 aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg ctatcgccgt
    54841 gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta aatttggcgc
    54901 tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca ggtttcagac
    54961 catcaccaat aaacggcaac gcacggtcca tgatcacgta catggagtag tttaagtagg
    55021 cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt aattctgatt
    55081 cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta cattatctgc
    55141 cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc gacggcgata
    55201 aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg catagagggc
    55261 agcacaaggt tgcgcccatg ccacaccact cggcatgggg gtccgctccg ggcgattcga
    55321 gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg actgtaagta
    55381 tacggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct gccataagtg
    55441 cggcttgctc atggatatcc ccgagacggc ttacggcgca ccggataata aatttcgcgt
    55501 atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc tgtgacattg
    55561 ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc
    55621 caacggcaca taaacatccg tttccagtcg ctgctctgtc accccttcgg caagattggt
    55681 gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca gccagtggcg
    55741 aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc gggccacggc
    55801 ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg gaatcggttc
    55861 gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg caggggcgtc
    55921 ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca
    55981 catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt gcggcaacat
    56041 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatttt
    56101 gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta
    56161 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    56221 gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg tgatcgatat
    56281 caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag atgcgttcaa
    56341 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacag
    56401 tcgttacgct gccgttcggc ccctgaaatc ctccggcctg actttgcgtt accgatgggc
    56461 cagaaaaacc gccctgctct gctgccatca ccggtgcact gcacagggcc attactgcga
    56521 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    56581 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc tgtacagagc
    56641 gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    56701 ttttactgat agaagatgac atgctgattg gtgacggcat caaaactggc cttagtaaaa
    56761 tgggttttag tgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    56821 caccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    56881 tgcgcgaatg gcgagagaaa ggtcagcgtg agccggtact gaacctgacc gcgcgtgatg
    56941 cgttagcgga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    57001 ttgcgttgat agaagtcgcc gccaagctgg aggctctgat gcgccgaacc aacggccagg
    57061 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    57121 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    57181 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    57241 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    57301 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    57361 ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    57421 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    57481 cgacacccaa ctgatgctgt ttgccaaacg gttaagtacg ctcgatctca acgaaatcaa
    57541 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    57601 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaatg atggcgataa
    57661 cggagaagat attccctata gctatcaacg ggaaggtttt gctgacgggc aactggtcgg
    57721 tgaagacgat ccttggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    57781 tgttggccag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    57841 gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac tactgggtcg
    57901 tgaactcgcg ccgctgaaca aactggcgct ggcactacgt atgcgtgacc ctgactcgga
    57961 aaaaccacta aacgcgactg gcgtacccag cgaagtgcgg ccactggttg agtcgctaaa
    58021 tcaactgttc gcccgcacac atgcgatgat ggttcgagaa cgacgcttta cctccgatgc
    58081 agctcacgaa ctacgtagcc cgttaacagc gctgaaagtg caaaccgaag ttgcgcagct
    58141 ctctgacgat gatccgcagg cgcggaaaaa agcactgctc caattacatt ccgggatcga
    58201 tcgcgctact cgtctggttg atcaactgct cacgctatcg cggctggact cactggataa
    58261 ccttcaggac gtcgcggaga tcccgcttga agatctccta caatcgtcgg tgatggatat
    58321 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg cccacggcat
    58381 caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgg
    58441 ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg
    58501 acaaaccgct accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca
    58561 tgacatgaat gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg
    58621 gtaacttctc tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa
    58681 atataaaccg tccccaaaaa agccaccaac cacaaaccag acaaataacg agcgagaata
    58741 catatagcca aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa
    58801 gtggcgtaag aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa
    58861 cggaaggtat actgcaaata ttgcctcccg actcaagcat ccagtcatac ttattgaaag
    58921 caaaaaaaga atctattaac caataagtaa gcattatata tagcaatgct tcaaatattg
    58981 ttttaatttt ctgctttaac atatatcagt acgctcataa aaatccattt ataattatca
    59041 acatgacacg ttgatatttt catcttttac ttattcaaag ccatattttc aatccccaaa
    59101 aaaaccgaac caaacgtgta aattattccg gtgcgagtaa gaattatgag gaatggctat
    59161 cagtattgtc attttcagaa aatatttatc ctgcatcggt gagtcagagc tagatcagac
    59221 ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcacagtac cgctctgtga
    59281 cgtactatag tcgacaaacg tctcaccttg agattaaaaa atgagcaaca tcctgattat
    59341 caacggcgcg aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt
    59401 cgcggatggc acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga
    59461 ctacaatgtc aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat
    59521 gccaggctgg tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac
    59581 cgaaggtcac gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa
    59641 atacggttcc ggcagcctgg tacagggcaa aaaatatatg ctttctctaa cctggaacgc
    59701 accaatggaa gccttcaccg aaaaagatca gttcttccac ggcgttggcg ttgacggtgt
    59761 gtatctgccg ttccataaag caaaccaatt cctcggtatg gaaccgctgc cgacatttat
    59821 cgctaatgac gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca
    59881 tcttgtggaa atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac
    59941 cgtaatcgca gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca
    60001 gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat
    60061 ggtggattgc gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat
    60121 tgagcagtgg gaaagcatcg cgcaccttga agcgcatctg caaaccccgc acatgaaggc
    60181 gtacagcgaa gccgtaaaag gcgacgtgct ggagatgaat atccgtattc tgcagccagg
    60241 gatttaatcc tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac
    60301 ctcaatctcc gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt
    60361 cttcgccagc agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt
    60421 caactgcacc agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt
    60481 catttccccc agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg
    60541 ctcaagtacg ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc
    60601 aatacggtag agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa
    60661 gtgttttacg aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc
    60721 cgcgaggata cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat
    60781 accgatcgct accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac
    60841 ttcccaggtg ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg
    60901 cgcctgcttg gcagatccgc ctgcggagtc accttccact aggaacagtt cggtacggtt
    60961 aaggccctgc gcggtacaat cagccagttt gccaggcagc gccgggccgc tggtcagctt
    61021 tttacgcacc acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc
    61081 tgccagcaac tccgccgcct gaacgttctg gttcagccac aggatgaagg catctttcac
    61141 cacgcccgaa acgaatgccg cacactgacg cgaagagaga cgctctttgg tctgcccggc
    61201 aaactgcgga tcctgcattt taaccgacag aacataggcg cagcgatccc agatatcttc
    61261 cgccgacagc ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc
    61321 gtccaacagg ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag
    61381 gttgacgtag ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc
    61441 cacagcttca gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa
    61501 accgtttacc gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc
    61561 ggtgttgttg atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc
    61621 cttcagcaca tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc
    61681 cggccagaag tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa
    61741 atcctgcacc ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac
    61801 gttaacttct acgcgcttcg acaaggcgtt aactaccgaa atccccacgc catgcaggcc
    61861 gccagagaac tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat
    61921 cagttcaacc gccggtactc cttcttccgg gtgaatatcc accggcatcc cgcgcccatc
    61981 gtcaatcact tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc
    62041 cgccagtgct tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt
    62101 ggtatcggta tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat
    62161 ggcatcagcg ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga
    62221 gatggtgcag accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg
    62281 cgtggttgcc gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc
    62341 ggtaatccag cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca
    62401 gcgggtcaat ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct
    62461 gcccggtgta ggggttctcg ttctgaccga gatagtccgt cagcaattca aacgggcgta
    62521 ccgccgggtt taccaccact gcgggcagca taaaacattg cgacaaccag gtggcgtaat
    62581 atccccccag tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg
    62641 attccagcag ttctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa
    62701 cgtcagggtg atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg
    62761 agctgttgaa accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc
    62821 aggttggaaa cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg
    62881 taactcgaga gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg
    62941 cttaaactgc acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc
    63001 cagctcctga tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac
    63061 ggtatccagt tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg
    63121 tagcggatga tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg
    63181 ttccagccac tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct
    63241 atccagcaac aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc
    63301 cgcatcctgt aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac
    63361 gcagggcgca cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg
    63421 atcctgcgct aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat
    63481 cgcctccagc accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt
    63541 ttgtgcaaac aggtgagtat cggtaatttg taaaatcctt cctctggcct caccagccag
    63601 aggaagggtt aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg
    63661 aatcgccatc gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat
    63721 ttgatgcttt tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa
    63781 gcgaaaaatc tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac
    63841 cgtcattgac ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcactag
    63901 ggtagtgtat cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata
    63961 gcttacagtt tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt
    64021 catctcgcac aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca
    64081 ctcatttttt aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc
    64141 gttgtcgatt ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac
    64201 gcgaatatct tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac
    64261 ttcgcccacc ataattgacg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa
    64321 caccggtttg gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac
    64381 atcttccaca ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt
    64441 ttcgctggtg tcgtacgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac
    64501 tgggtcaaag ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc
    64561 atgactcatt tgcccgttga atagacgatg acgaaatcta taaagatcta atgaaaaaaa
    64621 gccgcgataa agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg
    64681 caggttgtct ggcttaagca ttgttaatgt cctagcacta atagtgaatt aaatgtgaat
    64741 ttcagcgacg tttgactgcc gtttgagcag tcatatgtta aattgaggca cattaacgcc
    64801 ctatggcacg taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt
    64861 tacagtttga tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc
    64921 cccattctta tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg
    64981 atgcaagttt atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat
    65041 cgtgatgctg cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt
    65101 ttaggtgcag attacaccta tagcaacggc taccgcgacg cgaacggcat caactcgaac
    65161 gcgaccagtg cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg
    65221 ttaacgctgc aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa
    65281 accttgatcc tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt
    65341 tcctatacac aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt
    65401 tttaacgtgg gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca gtacgatacc
    65461 gtgctggcga acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc
    65521 cagatcaccg gtaactacta tccggaactg gcggcgctga atgtcgaaaa ctttaaaacc
    65581 gacaaaccac agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg
    65641 ttacaggcac gcttgagcca ggacctggag cgcgagcaaa ttcgccaggc gcaggatggt
    65701 cacttaccga ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt
    65761 tcgaaaaccc gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa
    65821 gttggcctga gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa
    65881 caggcacagt acaactttgt tggtgccagt gagcaactgg aaagcgcaca tcgtagcgtc
    65941 gtgcaaaccg tacgttcctc cttcaacaac attaatgctt ctatcagtag tattaacgcc
    66001 tacaaacaag ccgtagtttc cgcgcaaagc tcattagacg cgatggaagc gggctactcg
    66061 gtcggtacgc gtaccattgt tgatgtgttg gatgcgacca ccacgctgta caacgccaag
    66121 caagagctgg cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc
    66181 ctgggtacgt tgaacgagca ggatttgctg gcactgaaca atgcgctgag caaaccggtt
    66241 tccactaatc cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt
    66301 tatgcgcctg atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt
    66361 aacggtcata accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg
    66421 taaggcaacg taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc
    66481 gaccagccat cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa
    66541 cggacaaaat ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca
    66601 ccagtcgctc tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa
    66661 acagtgtctc tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc
    66721 gaatgtacca ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac
    66781 gccacccgtg aagactgtgt tgctgaattt ggtgaaggtc agtgtcagca agcaccagcc
    66841 caggctggca tggcaccaga aaaccaggcg caggctcagc aatccagcgg gagtttctgg
    66901 atgccgctga tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca
    66961 cagcagccgc tgttctcctc gaaaaacccg gccagtccgg cttacggtaa atataccgac
    67021 gcgacgggta aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg
    67081 gcaatggcac caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct
    67141 gttgccaaac aaagcactat gcagcgtagc gcaaccggca cctcttctcg ttcaatgggt
    67201 ggctgatacc gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc
    67261 acgaatacgg tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt
    67321 actacaagtt gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc
    67381 agatgtgcct gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc
    67441 gcataccaaa acacacctgg agttttgtgc gtcaatcatg gctgactcac cagccatcgc
    67501 tttattcgcg tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata
    67561 acgccgatac gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag
    67621 atcagcttaa cgccggtaac ttgtcggagg gcagcgacca gtttaacagt ctgcaagaaa
    67681 aactgatcga tcgcttcgtt gagctgcgtg aacagtatgg cttccagttg ctgcatctca
    67741 cctgctgtcg cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa
    67801 ccgaagctga aatcgccacc gagttcctct acatcgacga gataggttta ggcgaaaaag
    67861 gccagttcac ggatttacag gatcaggtga tttctaacct gttcaagctt tatccgtggg
    67921 aatttatgct gcgcgaaatg ttctccacca agctggaaga tgctggcgta cgctggctgg
    67981 aaccggcgtg gaagagcatt atctccaaca aggcacttct accgctactg tgggagatgt
    68041 tcccgaatca cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg
    68101 aaaaatatgt ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga
    68161 acggcaaaac cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc
    68221 aattccaccc gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga
    68281 acgatcaacc tgccggaatt ggcattcgtg aagaccgtgc gttgatcacc caggatatgt
    68341 ctcggtttta tccacatatt tttgttgaat aagccacgat accggatggc actcgccatc
    68401 cggtaattgt tagcctatct gcaccgacag catacttaaa ctgcccattt ctataccatc
    68461 gaccggaatg gtaattggct cctgcccatc ccacacacct aacacataca acaacggcag
    68521 atagtgctct ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag
    68581 aggatgctgc tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga
    68641 cgtcgcccac ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac
    68701 cacgttaccg ctggcgacca acattattcc ttcatcacgc agcgccgcga gtttgcgccc
    68761 catttcgaaa tgccaggcga caggtttgct actgtcgata ctcaactgca ccatagggat
    68821 atcggcgtca ggatacatct taatcagcac gccccacgag ccgtggtcaa agccccaggc
    68881 ttctttatcc agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc
    68941 aggcgaaccc ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc
    69001 atgaatcgtg ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc
    69061 cgaaaccacc acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg
    69121 ggtatacaaa ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg
    69181 catacgtgtt gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca
    69241 atacgcttgt tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt
    69301 gacgatctgc ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct
    69361 gaccatactg gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcggacaaaa
    69421 accctcgaac cgcttactgg cgttttcgct aggttttgcg gcggggatca tgttgctcat
    69481 ctcattaatg gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc cggtgttggg
    69541 ttatggaatg tttatcttcg gtctgcttgg ctactttggc ctggaccgca tgttgccgca
    69601 tgcgcatccg caggatttaa tgcaaaaatc ggtgcagctg ttgccaaaat cgatcaagcg
    69661 cacagccatt ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac
    69721 ctttgtcacg gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt
    69781 gcacaatatc cctgaaggtc tggcagtggc aggcccggtt tatgcggcaa cggggtctaa
    69841 acgcaccgcg attctgtggg cggggatttc tggactagca gaaattcttg gtggtgtgct
    69901 ggcgtggtta atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc
    69961 ggttgcagga attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat
    70021 tgaccctaat aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag
    70081 tttagtgctg ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt tgtccagcac
    70141 atggacaact tctgccagct tcacgaaagc cttattctac cttttcggct gtctcttctc
    70201 tcgtactgtt taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg
    70261 cccgacaata acgtttcaaa tgcaggaaat aacccaccac aaccagagca atcaatacaa
    70321 tccagtacca ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag
    70381 cgcataaatt tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca
    70441 attatatgat tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag
    70501 aaagagaagc ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac
    70561 aacttcggtt tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc
    70621 atgtgcctga cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt
    70681 ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca
    70741 cagcacacca gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg
    70801 accgccacgc gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg
    70861 attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacgatc
    70921 agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt
    70981 gccataggcg ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc
    71041 ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc
    71101 aacggtcatg gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc
    71161 aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa
    71221 aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc actaaaatta
    71281 tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat aacgtgagcg
    71341 ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt cggccccgga
    71401 attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg ctttcaactg
    71461 agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg attcactata
    71521 acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta tgacttaccc
    71581 ttatcgcact acaatggtac tcaacaccta tcagtacagg gaaactacaa tgattgaccc
    71641 gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag gaatcaggga
    71701 gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagctcagc tgacgcgcct
    71761 cgaccttgta agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac ggactcgtga
    71821 gaaactggcg ctgcttgaac aaagaatcag cgaactggaa aatcgcagta ctgagatcaa
    71881 aaagcagcca gatccagaga ctctcccccc aacgctgtaa acatttgtct ttaatgcact
    71941 gtaaattatg aattaatcct ctaatttaca gtgttttcta ttacattatc gcttaatccc
    72001 attcagagta tcatcacaaa aatgaatgat tgcaattgaa aatataattg ttatgtattc
    72061 tttaagtttt acttaatcga tatttagact ggccc
//
LOCUS       DADLCQ010000003        70704 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000003 DADLCQ010000000
VERSION     DADLCQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.003"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..794
                     /locus_tag="JQG95_000151"
     CDS             <1..794
                     /locus_tag="JQG95_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5886842.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="JQG95_000152"
     CDS             805..1896
                     /locus_tag="JQG95_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5886843.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTAAITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JQG95_000153"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="JQG95_000153"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY5886844.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /locus_tag="JQG95_000154"
     CDS             2864..4900
                     /locus_tag="JQG95_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY5886845.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="JQG95_000155"
     CDS             4858..5253
                     /locus_tag="JQG95_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY5886846.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="JQG95_000156"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="JQG95_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY5886847.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="JQG95_000157"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="JQG95_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY5886848.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="JQG95_000158"
     CDS             complement(6562..7602)
                     /locus_tag="JQG95_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5886849.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMALLIATLVQKWVRETLQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVCILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="JQG95_000159"
     CDS             complement(7675..8310)
                     /locus_tag="JQG95_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY5886850.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="JQG95_000160"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="JQG95_000160"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY5886851.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="JQG95_000161"
     CDS             complement(8936..9379)
                     /locus_tag="JQG95_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY5886852.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="JQG95_000162"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="JQG95_000162"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY5886853.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="JQG95_000163"
     CDS             complement(9719..10222)
                     /locus_tag="JQG95_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5886854.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="JQG95_000164"
     CDS             complement(10216..10740)
                     /locus_tag="JQG95_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY5886855.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="JQG95_000165"
     CDS             10949..11944
                     /locus_tag="JQG95_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY5886856.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="JQG95_000166"
     CDS             11953..12831
                     /locus_tag="JQG95_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY5886857.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="JQG95_000167"
     CDS             12912..13919
                     /locus_tag="JQG95_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY5886858.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="JQG95_000168"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="JQG95_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY5886859.1"
                     /translation="MATRTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /locus_tag="JQG95_000169"
     CDS             complement(15435..17324)
                     /locus_tag="JQG95_000169"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY5886860.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="JQG95_000170"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="JQG95_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY5886861.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="JQG95_000171"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="JQG95_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY5886862.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="JQG95_000172"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="JQG95_000172"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY5886863.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="JQG95_000173"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="JQG95_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY5886864.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="JQG95_000174"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="JQG95_000174"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY5886865.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="JQG95_000175"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="JQG95_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY5886866.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="JQG95_000176"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="JQG95_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY5886867.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="JQG95_000177"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="JQG95_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY5886868.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="JQG95_000178"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="JQG95_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY5886869.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="JQG95_000179"
     tRNA            complement(27653..27729)
                     /locus_tag="JQG95_000179"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="JQG95_000180"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="JQG95_000180"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY5886870.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="JQG95_000181"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="JQG95_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="JQG95_000182"
     tRNA            complement(31513..31599)
                     /locus_tag="JQG95_000182"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="JQG95_000183"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="JQG95_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY5886871.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="JQG95_000184"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="JQG95_000184"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY5886872.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="JQG95_000185"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="JQG95_000185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY5886873.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIEAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="JQG95_000186"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="JQG95_000186"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY5886874.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="JQG95_000187"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="JQG95_000187"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY5886875.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="JQG95_000188"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="JQG95_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY5886876.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="JQG95_000189"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="JQG95_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY5886877.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="JQG95_000190"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="JQG95_000190"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY5886878.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="JQG95_000191"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="JQG95_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY5886879.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKVIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="JQG95_000192"
     CDS             complement(41211..42176)
                     /locus_tag="JQG95_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5886880.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="JQG95_000193"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="JQG95_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY5886881.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="JQG95_000194"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="JQG95_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY5886882.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="JQG95_000195"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="JQG95_000195"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY5886883.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="JQG95_000196"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="JQG95_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY5886884.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="JQG95_000197"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="JQG95_000197"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY5886885.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="JQG95_000198"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="JQG95_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY5886886.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="JQG95_000199"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="JQG95_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY5886887.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="JQG95_000200"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="JQG95_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY5886888.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="JQG95_000201"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="JQG95_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY5886889.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="JQG95_000202"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="JQG95_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY5886890.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="JQG95_000203"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="JQG95_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY5886891.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="JQG95_000204"
     CDS             49714..50679
                     /locus_tag="JQG95_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY5886892.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="JQG95_000205"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="JQG95_000205"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY5886893.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="JQG95_000206"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="JQG95_000206"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY5886894.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="JQG95_000207"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="JQG95_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY5886895.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="JQG95_000208"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="JQG95_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY5886896.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="JQG95_000209"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="JQG95_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY5886897.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLISH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="JQG95_000210"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="JQG95_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY5886898.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="JQG95_000211"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="JQG95_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY5886899.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="JQG95_000212"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="JQG95_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY5886900.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="JQG95_000213"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="JQG95_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY5886901.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="JQG95_000214"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="JQG95_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY5886902.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58504
                     /gene="yrbL"
                     /locus_tag="JQG95_000215"
     CDS             57878..58504
                     /gene="yrbL"
                     /locus_tag="JQG95_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY5886903.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGGDKEIRRE
                     LKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCRYE
                     EDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLIPL
                     ATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="JQG95_000216"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="JQG95_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="JQG95_000217"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="JQG95_000217"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY5886904.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="JQG95_000218"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="JQG95_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY5886905.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLELNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="JQG95_000219"
     CDS             complement(62540..63469)
                     /locus_tag="JQG95_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY5886906.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAVRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="JQG95_000220"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="JQG95_000220"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY5886907.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /locus_tag="JQG95_000221"
     CDS             68617..70035
                     /locus_tag="JQG95_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5886908.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="JQG95_000222"
     CDS             70219..70698
                     /locus_tag="JQG95_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY5886909.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19179 c        17549 g        16662 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccgccattac cgtcatggtg cggtaaaaga agacgttacg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgca
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcagtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa cgccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctactgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt cgtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aagaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caagctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagttagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcttcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatca ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgataaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcacggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatctc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaccccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattca actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcacc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctggg
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgccg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaag
//
LOCUS       DADLCQ010000004        65614 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000004 DADLCQ010000000
VERSION     DADLCQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.004"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JQG95_000223"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JQG95_000223"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY5886910.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JQG95_000224"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JQG95_000224"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY5886911.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JQG95_000225"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JQG95_000225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY5886912.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JQG95_000226"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JQG95_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY5886913.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JQG95_000227"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JQG95_000227"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY5886914.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFCINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JQG95_000228"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JQG95_000228"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY5886915.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JQG95_000229"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JQG95_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY5886916.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JQG95_000230"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JQG95_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY5886917.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JQG95_000231"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JQG95_000231"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY5886918.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JQG95_000232"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JQG95_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY5886919.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JQG95_000233"
     CDS             complement(14847..15542)
                     /locus_tag="JQG95_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY5886920.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JQG95_000234"
     CDS             complement(15665..16702)
                     /locus_tag="JQG95_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5886921.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="JQG95_000235"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="JQG95_000235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5886922.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="JQG95_000236"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="JQG95_000236"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY5886923.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="JQG95_000237"
     CDS             19444..19884
                     /locus_tag="JQG95_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY5886924.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="JQG95_000238"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="JQG95_000238"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY5886925.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20636..21523)
                     /gene="ugpC"
                     /locus_tag="JQG95_000239"
     CDS             complement(20636..21523)
                     /gene="ugpC"
                     /locus_tag="JQG95_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY5886926.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="JQG95_000240"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="JQG95_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY5886927.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="JQG95_000241"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="JQG95_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY5886928.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVMLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="JQG95_000242"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="JQG95_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY5886929.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="JQG95_000243"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="JQG95_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY5886930.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="JQG95_000244"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="JQG95_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY5886931.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /locus_tag="JQG95_000245"
     CDS             complement(26622..27899)
                     /locus_tag="JQG95_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY5886932.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="JQG95_000246"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="JQG95_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY5886933.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livk"
                     /locus_tag="JQG95_000247"
     CDS             complement(28870..29979)
                     /gene="livk"
                     /locus_tag="JQG95_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY5886934.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30403..30786
                     /gene="panM"
                     /locus_tag="JQG95_000248"
     CDS             30403..30786
                     /gene="panM"
                     /locus_tag="JQG95_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY5886935.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30985..32088)
                     /locus_tag="JQG95_000249"
     CDS             complement(30985..32088)
                     /locus_tag="JQG95_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY5886936.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEELLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="JQG95_000250"
     CDS             complement(32359..33213)
                     /gene="rpoH"
                     /locus_tag="JQG95_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY5886937.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="JQG95_000251"
     CDS             complement(33458..34516)
                     /gene="ftsX"
                     /locus_tag="JQG95_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY5886938.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="JQG95_000252"
     CDS             complement(34509..35177)
                     /gene="ftsE"
                     /locus_tag="JQG95_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY5886939.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="JQG95_000253"
     CDS             complement(35180..36676)
                     /gene="ftsY"
                     /locus_tag="JQG95_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY5886940.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36826..37422
                     /gene="rsmD"
                     /locus_tag="JQG95_000254"
     CDS             36826..37422
                     /gene="rsmD"
                     /locus_tag="JQG95_000254"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY5886941.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37412..37681
                     /locus_tag="JQG95_000255"
     CDS             37412..37681
                     /locus_tag="JQG95_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY5886942.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37684..38043)
                     /locus_tag="JQG95_000256"
     CDS             complement(37684..38043)
                     /locus_tag="JQG95_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY5886943.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38184..38810
                     /locus_tag="JQG95_000257"
     CDS             38184..38810
                     /locus_tag="JQG95_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY5886944.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38884..41082
                     /gene="zntA"
                     /locus_tag="JQG95_000258"
     CDS             38884..41082
                     /gene="zntA"
                     /locus_tag="JQG95_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY5886945.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="JQG95_000259"
     CDS             complement(41184..41429)
                     /gene="tusA"
                     /locus_tag="JQG95_000259"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY5886946.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41650..42315
                     /locus_tag="JQG95_000260"
     CDS             41650..42315
                     /locus_tag="JQG95_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY5886947.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42388..42945
                     /gene="dcrB"
                     /locus_tag="JQG95_000261"
     CDS             42388..42945
                     /gene="dcrB"
                     /locus_tag="JQG95_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY5886948.1"
                     /translation="MRNLVKYVGIGLLVMGLVACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42949..44166)
                     /locus_tag="JQG95_000262"
     CDS             complement(42949..44166)
                     /locus_tag="JQG95_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5886949.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44300..45349
                     /locus_tag="JQG95_000263"
     CDS             44300..45349
                     /locus_tag="JQG95_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY5886950.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45404..45991
                     /gene="acpT"
                     /locus_tag="JQG95_000264"
     CDS             45404..45991
                     /gene="acpT"
                     /locus_tag="JQG95_000264"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY5886951.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46102..47676
                     /gene="nikA"
                     /locus_tag="JQG95_000265"
     CDS             46102..47676
                     /gene="nikA"
                     /locus_tag="JQG95_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY5886952.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47676..48620
                     /gene="nikB"
                     /locus_tag="JQG95_000266"
     CDS             47676..48620
                     /gene="nikB"
                     /locus_tag="JQG95_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY5886953.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48617..49450
                     /gene="nikC"
                     /locus_tag="JQG95_000267"
     CDS             48617..49450
                     /gene="nikC"
                     /locus_tag="JQG95_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY5886954.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49450..50214
                     /gene="nikD"
                     /locus_tag="JQG95_000268"
     CDS             49450..50214
                     /gene="nikD"
                     /locus_tag="JQG95_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY5886955.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50211..51017
                     /gene="nikE"
                     /locus_tag="JQG95_000269"
     CDS             50211..51017
                     /gene="nikE"
                     /locus_tag="JQG95_000269"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY5886956.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51023..51424
                     /gene="nikR"
                     /locus_tag="JQG95_000270"
     CDS             51023..51424
                     /gene="nikR"
                     /locus_tag="JQG95_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY5886957.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51544..51903)
                     /locus_tag="JQG95_000271"
     CDS             complement(51544..51903)
                     /locus_tag="JQG95_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY5886958.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51900..52175)
                     /locus_tag="JQG95_000272"
     CDS             complement(51900..52175)
                     /locus_tag="JQG95_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY5886959.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52234..53358)
                     /locus_tag="JQG95_000273"
     CDS             complement(52234..53358)
                     /locus_tag="JQG95_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY5886960.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="JQG95_000274"
     CDS             complement(53358..56093)
                     /gene="rbbA"
                     /locus_tag="JQG95_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY5886961.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56090..57157)
                     /locus_tag="JQG95_000275"
     CDS             complement(56090..57157)
                     /locus_tag="JQG95_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5886962.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57523..59145)
                     /locus_tag="JQG95_000276"
     CDS             complement(57523..59145)
                     /locus_tag="JQG95_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY5886963.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKDYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTKPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59407..60996)
                     /locus_tag="JQG95_000277"
     CDS             complement(59407..60996)
                     /locus_tag="JQG95_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY5886964.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61493..62412
                     /locus_tag="JQG95_000278"
                     /pseudo
     CDS             61493..62412
                     /locus_tag="JQG95_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(62499..63014)
                     /locus_tag="JQG95_000279"
     CDS             complement(62499..63014)
                     /locus_tag="JQG95_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886965.1"
                     /translation="MTKLMQFVQRCYYMTNKKMYFILILVFTLLQVCFFALWKARDGS
                     TTSLECTSTLTRNAKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFF
                     TYKIDSNGRISGNAKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSI
                     PGLIINTCAPI"
     gene            complement(62968..63732)
                     /locus_tag="JQG95_000280"
     CDS             complement(62968..63732)
                     /locus_tag="JQG95_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5886966.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEIFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63978..64739
                     /locus_tag="JQG95_000281"
     CDS             63978..64739
                     /locus_tag="JQG95_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5886967.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDSFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64846..65358)
                     /locus_tag="JQG95_000282"
     CDS             complement(64846..65358)
                     /locus_tag="JQG95_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886968.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
BASE COUNT        15937 a        17619 c        16161 g        15897 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgcag aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgacacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gctagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcaacacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc atgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg gagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggctagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaagatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat
    30241 tcaggggatt atgctggaca tttttcattc tctaatgttt taactttgta attattgctg
    30301 ttaaaaaatt aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt
    30361 tcgctacact ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt
    30421 cgattagaaa actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat
    30481 tccccttcct ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag
    30541 cgtctgcttg cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta
    30601 cgcgtgcgag aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg
    30661 cgtaacaatc ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt
    30721 gtgatgacgg cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag
    30781 cgttaatcgt caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac
    30841 gcttatcagg cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg
    30901 ataaggcgtt tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg
    30961 caacccaaag ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca
    31021 gtcaaatacg ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc
    31081 catcacggta tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga
    31141 ctgattcagg cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc
    31201 gcttgggtcc tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg
    31261 atcgtagttc ttcggcttgg tcaccagcag ctcttccgct gattcgcccg caatgttaga
    31321 cagcgaaacg ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg
    31381 tgcctgacgc aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat
    31441 attctctttt ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc
    31501 atcaaagaac accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag
    31561 accttcgccg tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc
    31621 aagaatatat ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat
    31681 cagctgatag ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat
    31741 gccttcgtct tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat
    31801 cacatattta atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc
    31861 acaggcatcg tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt
    31921 gatatccgca accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg
    31981 accggacatt gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa
    32041 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
    32101 cattcttctg gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat
    32161 gcatgactac ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat
    32221 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
    32281 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
    32341 tctgcttaat agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt
    32401 ttccagttgg cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
    32461 ggacttgttg tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag
    32521 accatgcatc gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat
    32581 gccgtcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
    32641 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
    32701 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc
    32761 ctggttaaac cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg
    32821 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
    32881 tgctttgatc cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
    32941 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
    33001 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
    33061 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
    33121 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
    33181 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
    33241 gcgcttcagt ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact
    33301 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
    33361 caaaatgtga catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac
    33421 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
    33481 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca
    33541 atagcaggca ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg
    33601 ccacttccgc caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca
    33661 acaatgcgcc agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac
    33721 catccgtcgc accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca
    33781 gacgtacgct gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg
    33841 ccgaaacgcg cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca
    33901 tccgcacttc gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt
    33961 ccgtcccctg aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt
    34021 ccagcatatc cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt
    34081 cacgagaaag atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc
    34141 ccgcagcggc gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat
    34201 aatactgcgt cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg
    34261 tcagggagat ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat
    34321 cctgcaatgc gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat
    34381 ttaccggttt tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc
    34441 cgccgaccga tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg
    34501 catcgcgctt attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg
    34561 cgataggaac gccgtgaaat caggttaatg tcgtgcgttg ccatcaatac ggttacccca
    34621 acgcggttaa actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta
    34681 ccggtcggtt cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca
    34741 acacgctgtt gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc
    34801 ccgactttat ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg
    34861 ataatcagcg ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc
    34921 tggaaaatca tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg
    34981 atgtcatggc cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc
    35041 ttcaggaggg tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc
    35101 tgcatatgga acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca
    35161 tgttcaaagc gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg
    35221 ccttaaacgg acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac
    35281 caaactggtc agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta
    35341 gcgtgatgcc ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct
    35401 gcccggtgct ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct
    35461 tcatcacgcg gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc
    35521 cggcggtatc ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga
    35581 agataacaga ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct
    35641 gaccccagac ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca
    35701 tcaccgattt accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac
    35761 ccacaccgtt gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg
    35821 gctcatcgac tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct
    35881 cggcatcacg aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg
    35941 tggtttccac gcccacatcg gcgatcaaca gctgttcttc cagctcttca aatagatcat
    36001 cgtcgatttt tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt
    36061 ttaacaggct gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct
    36121 gggcaatttc tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt
    36181 ctgccgcttc ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag
    36241 ccgcttcttc ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt
    36301 ctggcgaaac ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag
    36361 ccactggctc cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag
    36421 gctgcgcttt ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag
    36481 tttccgcctc ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct
    36541 tcgcaggctc ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg
    36601 tctctttttc cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc
    36661 ttttttcttt cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac
    36721 gctatgtaca tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat
    36781 caccgcctgc aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat
    36841 cattccggca gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg
    36901 gttcctgata gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac
    36961 tggctggctc cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg
    37021 ctggggctgg aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc
    37081 gtggtttctc agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg
    37141 gtgaacagca acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt
    37201 gtcgatccac cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac
    37261 ggctggctgg ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc
    37321 actgttccgg caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg
    37381 ctgtatcaac acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat
    37441 gctctgcgtt tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa
    37501 tattttcgtt aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct
    37561 attgaaatcc actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat
    37621 tttccttttc ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata
    37681 accttattgt tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt
    37741 atcgcccact gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc
    37801 tccgctttgc ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc
    37861 ctgctggcgc gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag
    37921 cttttgctgg agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg
    37981 aaacgatgcg gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact
    38041 catctttatc cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt
    38101 catccgtcca ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa
    38161 aataacgaat tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat
    38221 ggctatctgt ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt
    38281 taacccttct tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct
    38341 atctggtgct ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac
    38401 gtcaacgtct gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct
    38461 atttcgccag tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc
    38521 tgggtgcgct gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct
    38581 gcacgtttat cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc
    38641 gtccgaccgc tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact
    38701 ttgtctggct ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg
    38761 cctgctactt tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga
    38821 ctctggagtc gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat
    38881 gccatgtcga cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg
    38941 ctaaccacgg tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca
    39001 acgctctctg aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc
    39061 gccgcctgtg cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg
    39121 caggtgttgt tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag
    39181 gttgaatctg cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag
    39241 ccacaagcat cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca
    39301 atcagctggg gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc
    39361 acgctagttg ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc
    39421 tacttcgcca ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca
    39481 acggctgaag ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg
    39541 gccgccagcc gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc
    39601 acgcgcctgc gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat
    39661 gtgattgaag tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt
    39721 gccagttttg atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc
    39781 gataaagttc ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca
    39841 gaaccgggtg ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc
    39901 cgcgccccca ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg
    39961 gccgtcgccc tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag
    40021 tggatttata aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca
    40081 acgcctgcgg cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa
    40141 ggcggtgcgg cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt
    40201 acgctgaccg tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa
    40261 tctgaactgc tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa
    40321 gccatcgtac gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg
    40381 gcactggtcg ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct
    40441 gccgggaaac atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg
    40501 caaacggttg tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat
    40561 accctgcgcg ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga
    40621 gtgatcctca ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg
    40681 gagtttaaag cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa
    40741 catgcgcccc tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc
    40801 gccatcggga ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca
    40861 ttaacccata accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac
    40921 gccaatatcc gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc
    40981 acgcttttag ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg
    41041 ctggtgacgg cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca
    41101 ttgagcgcga tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg
    41161 gcatcgaagc caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt
    41221 ccatccgtct ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg
    41281 cgggtagtgg ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc
    41341 acggttttgc gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg
    41401 tggtcagggc tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta
    41461 gtttacgccc tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt
    41521 gcattgtcaa cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta
    41581 tgttaaggta acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa
    41641 aggtacaata tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg
    41701 ttatttcatc ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt
    41761 tttggtttcc ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac
    41821 ctgaccgtgc gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc
    41881 cctgcgttat taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga
    41941 ttcggcgcac tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg
    42001 gcctacgcgc tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc
    42061 cgctggtggc tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc
    42121 tttttcttca ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa
    42181 atcgcgctgg tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg
    42241 tatggcgtat tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct
    42301 ttgcaggcga gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta
    42361 tggccgttta tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc
    42421 ctgctggtta tggggcttgt ggcctgtgat gataaagaca ctaacgctac ggcgcagggt
    42481 tcggttgcgg aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt
    42541 ttctcgctgc cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac
    42601 atgcatgtct ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat
    42661 ccgaaagaag atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat
    42721 ccgcagctgc aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag
    42781 ttagacagta ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac
    42841 gtgggtaatc aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg
    42901 caaaccaccg cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga
    42961 ggcggcctca gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc
    43021 gattgcgacc agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac
    43081 cagcccagcc agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc
    43141 cagcgccgcc ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg
    43201 gaacaccagc gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat
    43261 agtcgccacg ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac
    43321 gtttaagcca ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc
    43381 acagctaaac agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag
    43441 cgtgataaag gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta
    43501 cagccagacg cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac
    43561 cgtcggacgc gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc
    43621 taacgcctgc aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat
    43681 cgccccgtaa gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac
    43741 cactccaacg ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat
    43801 gacgcgcccc aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc
    43861 cgtcagatac cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt
    43921 gggtcccagc aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata
    43981 ttgcaggctg ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata
    44041 gcccggtaat acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac
    44101 aatggagaca atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc
    44161 gggcatgagg atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga
    44221 aaatcagcag taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa
    44281 aagtcacaag gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct
    44341 gctcaagttg gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat
    44401 tgtgcagctg ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt
    44461 tattcgtcgg ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat
    44521 cgcactaacc gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct
    44581 gccgaagttt aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt
    44641 tcttaatttg catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt
    44701 taccttcacc acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct
    44761 ggtgatgacc gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt
    44821 tgcgctgaat aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc
    44881 gcattatctg gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg
    44941 gctggcgcta atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa
    45001 ctacgtgccc aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct
    45061 gtttaatggt gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt
    45121 catcggcaat attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt
    45181 ggtatttctt tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc
    45241 ggtgccatta accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact
    45301 ggcgatttta ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg
    45361 atacgatacg cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct
    45421 ggggaaagtt tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc
    45481 gcaaggtcca cgacgcgaac gctggctggc ggggcgtgca ttgctttcgc acacgctttc
    45541 cccgctaccg gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc
    45601 gctatggttc aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg
    45661 cgaagtcggc tgcgatatcg aagtgattcg cccgcgcgcc aactggcgct ggctggcgaa
    45721 cgccgtgttc agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact
    45781 ggaaatgttc tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag
    45841 cgcctggcaa atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg
    45901 tcagctagaa aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga
    45961 cagtgtgcaa tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat
    46021 ccagaacagg taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta
    46081 ttttaatcca ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg
    46141 gcttgtgcgt cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg
    46201 gtgaatgtcg ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc
    46261 atggtttatg aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca
    46321 aaaagctgga ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg
    46381 aaattctcca acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg
    46441 ctcgataacc gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa
    46501 gcactcaata aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa
    46561 gaactggccc tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa
    46621 accatgaacg gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa
    46681 ctgaatcagt acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt
    46741 aaaaagatca cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact
    46801 ggcgatatcg acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc
    46861 tttagccaga atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg
    46921 gcgctcaata ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac
    46981 gcggtaaaca aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac
    47041 accctgtttg ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat
    47101 ccgcagaaag cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac
    47161 atccgcgaga aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg
    47221 ttaagcaaat cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc
    47281 tcgctgattg gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc
    47341 atgattttcc accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg
    47401 cgcgtaccgt cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt
    47461 gataaagaga tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat
    47521 cgcgacattc tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg
    47581 atgatggtgg tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa
    47641 attccgttcg aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct
    47701 ttctgctgct gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc
    47761 tcggtaccgg cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg
    47821 agatgctggc ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg
    47881 gtacctggtt gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc
    47941 cggtactgga cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc
    48001 tggtattaat tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc
    48061 gtttgccgga tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct
    48121 ggctggcatt tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg
    48181 gctacggcag ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg
    48241 cgattaacgc gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca
    48301 cctgggcgcg gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca
    48361 atgcctcgct gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga
    48421 cgatgattat cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga
    48481 tttttaaccg tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg
    48541 tggtctgtaa tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc
    48601 atgaaggagc gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct
    48661 gatcattatc gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga
    48721 tccacaggca attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg
    48781 caccgatcac ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct
    48841 cggttcggta atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag
    48901 cgccgggttg attggcgggc gcgttgatca ggccaccatg cgcgtcgccg atatgtttat
    48961 gaccttcccg acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac
    49021 caacgtaatt atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag
    49081 cttggtgatt tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg
    49141 ccatgtgcgg gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct
    49201 ggcaacgctg gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct
    49261 cggtgtgacc gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat
    49321 ctggacccag ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc
    49381 ctttaacctg gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca
    49441 cgcacactaa tgccgcaaca gattgaacta cgtaatatcg cgctacaggc cgcgcagccg
    49501 ctggtacacg gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt
    49561 agcggcagcg ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt
    49621 cgccagacgg cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc
    49681 ggcatcaaaa ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc
    49741 atgcacaccc acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg
    49801 cttaccgctg ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac
    49861 ccgttcgaga tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt
    49921 gaatcaccgt ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg
    49981 cgcatcctcg atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc
    50041 acccatgata tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt
    50101 aaaattgtcg aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg
    50161 cgcagcctgg tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac
    50221 ttaacgtctg cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac
    50281 atcaggcggt gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc
    50341 tgggacgcag cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc
    50401 cctcacaagg gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac
    50461 gtaaagcctt ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc
    50521 cgcgcaaaac cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa
    50581 aatccgaaca actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg
    50641 ttctcgacaa acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac
    50701 gcgcgctggc ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc
    50761 tcgtgttaca ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg
    50821 cctgcctgtt catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg
    50881 tgatggacaa cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct
    50941 ctgacgccgg acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca
    51001 cctcagaaaa ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact
    51061 ggagacgctg gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg
    51121 cgatattctg cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt
    51181 cgcggtgctg tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc
    51241 cacccagcat catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga
    51301 cgactgtctg gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga
    51361 tgacgttatc gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga
    51421 ttgagttatt tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta
    51481 agttcagttg catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa
    51541 aatttataaa tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac
    51601 ttgttgctgc gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt
    51661 aaaggtctta atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc
    51721 aagatactct cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac
    51781 aaaatcacaa taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac
    51841 gtaggtaata acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat
    51901 catggtttta cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg
    51961 gtatctggcg acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg
    52021 caacgcgaac ctcttccttt cttaatttct ccgcctaatg ctttaaccag tgactctata
    52081 tcagaccact tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt
    52141 tgtttcttcc gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta
    52201 tgacatcaca gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct
    52261 gaatcgcagc agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg
    52321 cggccagacg atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa
    52381 gtgtgtcgtc ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg
    52441 cgtggatcca ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag
    52501 ttgcggcatt gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag
    52561 actgagcgcc acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac
    52621 acctttcacc atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga
    52681 ccagatcttc gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac
    52741 cgtgccgtgt tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt
    52801 aatgttgttg atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa
    52861 gcgcatccgg gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa
    52921 gctgttcact tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct
    52981 catgcgcgtg gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg
    53041 ctgaaaatta ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc
    53101 catctcatcg gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac
    53161 gatccggttc gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa
    53221 cgatcctggc gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag
    53281 cgtcagcatc gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat
    53341 attgcgtaaa tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc
    53401 accagcggga tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc
    53461 gctttcgaga acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg
    53521 atccaacgtc caagcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc
    53581 gccgggatca atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag
    53641 gtggagatca gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg
    53701 agcgtcagga aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc
    53761 aggaaaaagt tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg
    53821 ctacgggtgg tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc
    53881 acgctaaggg cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga
    53941 acaatcgctg gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc
    54001 agcccgcttt ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg
    54061 tgcattgcct gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc
    54121 cagacgccga gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc
    54181 tcaatggcca ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg
    54241 agcggcggct gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta
    54301 ctgacggtct ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg
    54361 ccgtaaccca ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact
    54421 ggatcgcgtc gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta
    54481 aatccctgac gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg
    54541 ctctgccctg ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg
    54601 cgtttctcaa ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt
    54661 gagatgcggt cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact
    54721 ttgtcctggc gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg
    54781 acaccagaag taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc
    54841 agcgaaagcc gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg
    54901 ttgagcttaa aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg
    54961 tgaaacaaac gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa
    55021 aacgcctgcg acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt
    55081 tgcccaaata gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg
    55141 gtggtggatt taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg
    55201 cggaaattga cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct
    55261 tcgatggcaa tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc
    55321 tggcgttgcg cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt
    55381 tgctgccgca gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc
    55441 agccagtcga agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg
    55501 ctctgccgct ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca
    55561 acccccgttg ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac
    55621 agcccaagtt tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt
    55681 gctaacccgg tgctggtcag cagctcatta attcgcactt cccgctccgc tttatcgtga
    55741 ccgaacaggc gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt
    55801 ttgcccagcc cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg
    55861 tcgtgcatat cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa
    55921 atcagtgaca ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa
    55981 cgggccggaa tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc
    56041 acgcccgcca gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca
    56101 acctcaccac gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc
    56161 ccggtaaacc ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg
    56221 ctttgacgcg gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga
    56281 actgggcgac actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat
    56341 cgaggatcag ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga
    56401 agaaggtcat atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca
    56461 gcacttcccc tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt
    56521 cactgtcatc gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag
    56581 tttgcgcctg aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct
    56641 gggctttcgc tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca
    56701 gctgttgcgc agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg
    56761 caacagagtc cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac
    56821 tttgtcgttg ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt
    56881 gcgcgatggc ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt
    56941 cgccttcgcg aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt
    57001 tgctggcgat atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg
    57061 gcacacccgc cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga
    57121 caacccacca cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt
    57181 aagcaaacga cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg
    57241 gataagaagg aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg
    57301 cttagtgaaa acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac
    57361 gcatactcat aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat
    57421 cgcatttcac gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag
    57481 tctcaaccag atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt
    57541 gcgccttcta atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca
    57601 gggagtggtt atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat
    57661 tcttccagca tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat
    57721 gcgttctctc ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga
    57781 acatgctaat agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt
    57841 aaaggatcga caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg
    57901 atgcttttta ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc
    57961 aatagagaca gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc
    58021 atgatgattt gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat
    58081 cccgtcgtag gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc
    58141 attgcaattc gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat
    58201 gcgttttaac aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt
    58261 ttggtcgcca agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg
    58321 aagactttct aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt
    58381 gtcaagttgc tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt
    58441 aagcaactgg ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa
    58501 aagcgcgtac aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcttggt
    58561 acttgtggga tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt
    58621 tataattgtt tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat
    58681 gggtttgtac gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt
    58741 aagtaaataa acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatc
    58801 tttactatta attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg
    58861 catgcccgat agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt
    58921 gtcatcttga atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac
    58981 ttcaccgagc attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata
    59041 cccaaacagt tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat
    59101 tgtcgttgta ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt
    59161 attcaccgcg aggaatacat agttaacatt ttatcttatg acatacacct catattcgat
    59221 ggggaaataa ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt
    59281 atctcatagc agagaaagtc cctaaagatt gtagaggcgt catcagtaca tacctctaca
    59341 atcgattaag aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat
    59401 gaagttttat tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc
    59461 gttaggtgct atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg
    59521 gtctacccct aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca
    59581 tttgaagata tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata
    59641 tacaggtgtg ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg
    59701 ttgtttatgt tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat
    59761 ctgattaaat ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat
    59821 tattccgtga tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt
    59881 tttaattaag ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata
    59941 gttaccatta aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg
    60001 gctatcttta ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc
    60061 accaaaaata gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat
    60121 cgcatcgtcc tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt
    60181 taataaattg tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc
    60241 gtgttttaat aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga
    60301 aacgagcaat gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt
    60361 gttggcattt tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac
    60421 agatttaaca tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg
    60481 ccagtcagct ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag
    60541 cccattaact ttataaatta tgtctgattt atctccggag aaattaataa aataataacc
    60601 atccttacac tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc
    60661 tggtataatt gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg
    60721 aatatctata gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc
    60781 atttaccctc cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc
    60841 tatttgcgaa tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc
    60901 aaccttgcta tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga
    60961 agggatgtac ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc
    61021 tgttagtaaa aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat
    61081 aatacttata tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg
    61141 gaaaacaatc catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct
    61201 taggataaat atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt
    61261 aaaccccgct atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac
    61321 aggctgaaat tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa
    61381 gtgaacatat atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc
    61441 atcgcgttag gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat
    61501 ttctctggcg gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact
    61561 cacgcgcggc gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc
    61621 aattatcacc atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga
    61681 gtttgttgcc ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt
    61741 ggcagcgtcg tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga
    61801 cggaacaccg gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt
    61861 tttactcacc ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac
    61921 tccgcattat gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc
    61981 acaatcgtcc atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat
    62041 tgggttattt ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc
    62101 gatgcaagcg cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac
    62161 agcatattct caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc
    62221 aacagaatac cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc
    62281 ctcgcggaaa tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga
    62341 ttaggcgcag tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg
    62401 aaaaaaaatt aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac
    62461 gaacttagtg aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta
    62521 atgatcaacc ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg
    62581 cctttacctg ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga
    62641 ttttccagtc ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt
    62701 ttgtaggtaa aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca
    62761 acaatcgtaa aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag
    62821 tataaagaat gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt
    62881 gtgctaccat cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca
    62941 agtattaata taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc
    63001 ataagtttcg tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag
    63061 caaataatgc atactattta catcatatat aaaatttgca cctggcacac agggtaaatg
    63121 ctttttcgaa atttcagcgg cctctatttg agatttagcg aggccaatct cgttaagatt
    63181 ctctgaaaga tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt
    63241 tatactgtag gtatctttaa aagccacggc aaatatttca ataataacaa ttactaccac
    63301 aagcgaaatc aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac
    63361 atcttcttgt aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat
    63421 gtaaaagcct actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa
    63481 acttttgcgt aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa
    63541 tctctccaaa acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag
    63601 taacactgcc aaaatacacc ccgccgtagc tggcaaattt atctcatcct gggtatttac
    63661 attcgagagc gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca
    63721 tctccgctcc atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg
    63781 tggaactgac acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta
    63841 tatccacaga ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga
    63901 aaagtgatgc aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca
    63961 tgtaatttca tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg
    64021 tcttactcat gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa
    64081 acgcaaaagg cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta
    64141 aatcttttta ttgttcaccg gtgtgcacga cagttgaact catctcacaa aataattgtt
    64201 ttgctatggg tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta
    64261 aagccttctc tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg
    64321 tcgctgatac ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga
    64381 cacctatcgt catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac
    64441 tgggaggagt taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca
    64501 atgattcttt taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc
    64561 aacaaattga gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg
    64621 caatgaatat tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg
    64681 gaattaagaa aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat
    64741 gtaaaaatca ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa
    64801 aggcgggata tcaaaccccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat
    64861 ataagcctgg gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga
    64921 aacgacgcga taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc
    64981 aatctcttcg gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag
    65041 tttatggtcc gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc
    65101 ataaatatca tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac
    65161 aatacctgtt gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg
    65221 ttgaatggta ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt
    65281 accctggctt actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc
    65341 taacaatgtt ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt
    65401 ttctttcttt gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct
    65461 tcacaaaaat aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg
    65521 gtataaccct gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc
    65581 tttagaataa ccccatttca cattaaactc gcct
//
LOCUS       DADLCQ010000005        65614 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000005 DADLCQ010000000
VERSION     DADLCQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.005"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="JQG95_000283"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="JQG95_000283"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY5886969.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQG95_000284"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="JQG95_000284"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY5886970.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQG95_000285"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="JQG95_000285"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY5886971.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /locus_tag="JQG95_000286"
     CDS             complement(3024..3479)
                     /locus_tag="JQG95_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY5886972.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQG95_000287"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="JQG95_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY5886973.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQG95_000288"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="JQG95_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY5886974.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="JQG95_000289"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="JQG95_000289"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY5886975.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="JQG95_000290"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="JQG95_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY5886976.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRVRLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="JQG95_000291"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="JQG95_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY5886977.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="JQG95_000292"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="JQG95_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY5886978.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="JQG95_000293"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="JQG95_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY5886979.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="JQG95_000294"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="JQG95_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY5886980.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQG95_000295"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="JQG95_000295"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY5886981.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="JQG95_000296"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="JQG95_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY5886982.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="JQG95_000297"
     CDS             19304..19369
                     /locus_tag="JQG95_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5886983.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="JQG95_000298"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="JQG95_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY5886984.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="JQG95_000299"
     CDS             complement(19753..20376)
                     /locus_tag="JQG95_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY5886985.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="JQG95_000300"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="JQG95_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY5886986.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="JQG95_000301"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="JQG95_000301"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY5886987.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="JQG95_000302"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="JQG95_000302"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY5886988.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSCLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="JQG95_000303"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="JQG95_000303"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY5886989.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /locus_tag="JQG95_000304"
                     /pseudo
     CDS             25215..26371
                     /locus_tag="JQG95_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26460..26846)
                     /locus_tag="JQG95_000305"
     CDS             complement(26460..26846)
                     /locus_tag="JQG95_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY5886990.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="JQG95_000306"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="JQG95_000306"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY5886991.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="JQG95_000307"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="JQG95_000307"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY5886992.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="JQG95_000308"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="JQG95_000308"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY5886993.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="JQG95_000309"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="JQG95_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY5886994.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="JQG95_000310"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="JQG95_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY5886995.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="JQG95_000311"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="JQG95_000311"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY5886996.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDSTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="JQG95_000312"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="JQG95_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY5886997.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="JQG95_000313"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="JQG95_000313"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY5886998.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="JQG95_000314"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="JQG95_000314"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY5886999.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="JQG95_000315"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="JQG95_000315"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY5887000.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="JQG95_000316"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="JQG95_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY5887001.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPGSKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="JQG95_000317"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="JQG95_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY5887002.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDVGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="JQG95_000318"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="JQG95_000318"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY5887003.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="JQG95_000319"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="JQG95_000319"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY5887004.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="JQG95_000320"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="JQG95_000320"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY5887005.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="JQG95_000321"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="JQG95_000321"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY5887006.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="JQG95_000322"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="JQG95_000322"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY5887007.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="JQG95_000323"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="JQG95_000323"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY5887008.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="JQG95_000324"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="JQG95_000324"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY5887009.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTMRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="JQG95_000325"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="JQG95_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY5887010.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="JQG95_000326"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="JQG95_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY5887011.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYHNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCPV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="JQG95_000327"
     CDS             53914..54303
                     /locus_tag="JQG95_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5887012.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="JQG95_000328"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="JQG95_000328"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY5887013.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLKLPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="JQG95_000329"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="JQG95_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY5887014.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="JQG95_000330"
     CDS             complement(55962..56162)
                     /locus_tag="JQG95_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY5887015.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="JQG95_000331"
     CDS             56328..56873
                     /locus_tag="JQG95_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY5887016.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="JQG95_000332"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="JQG95_000332"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY5887017.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="JQG95_000333"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="JQG95_000333"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY5887018.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="JQG95_000334"
     CDS             complement(58171..58995)
                     /locus_tag="JQG95_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY5887019.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQG95_000335"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="JQG95_000335"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY5887020.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQG95_000336"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="JQG95_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY5887021.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQG95_000337"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="JQG95_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY5887022.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQG95_000338"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="JQG95_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY5887023.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQG95_000339"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="JQG95_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY5887024.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQG95_000340"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="JQG95_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY5887025.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="JQG95_000341"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="JQG95_000341"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY5887026.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15507 a        16261 c        18085 g        15761 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca gacggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatcag gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgtgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgctcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggtgcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagctgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaccaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcagcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgtggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgatagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagattcaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta ccctggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttggc agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg ggagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc agctgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gtgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt cattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctatgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgacggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacatctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaacatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctacca
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgtcccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctgaaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacttggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagcaat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa agaggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgc
//
LOCUS       DADLCQ010000006        61491 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000006 DADLCQ010000000
VERSION     DADLCQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.006"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(20..907)
                     /gene="rpnA"
                     /locus_tag="JQG95_000342"
     CDS             complement(20..907)
                     /gene="rpnA"
                     /locus_tag="JQG95_000342"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY5887027.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
     gene            complement(1110..1346)
                     /gene="feoC"
                     /locus_tag="JQG95_000343"
     CDS             complement(1110..1346)
                     /gene="feoC"
                     /locus_tag="JQG95_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY5887028.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1346..3667)
                     /gene="feoB"
                     /locus_tag="JQG95_000344"
     CDS             complement(1346..3667)
                     /gene="feoB"
                     /locus_tag="JQG95_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY5887029.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3684..3911)
                     /gene="feoA"
                     /locus_tag="JQG95_000345"
     CDS             complement(3684..3911)
                     /gene="feoA"
                     /locus_tag="JQG95_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY5887030.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4349..6670)
                     /locus_tag="JQG95_000346"
     CDS             complement(4349..6670)
                     /locus_tag="JQG95_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY5887031.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSETGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6767..7279)
                     /gene="greB"
                     /locus_tag="JQG95_000347"
     CDS             complement(6767..7279)
                     /gene="greB"
                     /locus_tag="JQG95_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY5887032.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            7471..8190
                     /gene="ompR"
                     /locus_tag="JQG95_000348"
     CDS             7471..8190
                     /gene="ompR"
                     /locus_tag="JQG95_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY5887033.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            8187..9539
                     /gene="envZ"
                     /locus_tag="JQG95_000349"
     CDS             8187..9539
                     /gene="envZ"
                     /locus_tag="JQG95_000349"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY5887034.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9615..11237)
                     /gene="pckA"
                     /locus_tag="JQG95_000350"
     CDS             complement(9615..11237)
                     /gene="pckA"
                     /locus_tag="JQG95_000350"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY5887035.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11616..13340
                     /locus_tag="JQG95_000351"
     CDS             11616..13340
                     /locus_tag="JQG95_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY5887036.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTPMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     FAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(13484..14353)
                     /gene="hslO"
                     /locus_tag="JQG95_000352"
     CDS             complement(13484..14353)
                     /gene="hslO"
                     /locus_tag="JQG95_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY5887037.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14378..14779)
                     /gene="hslR"
                     /locus_tag="JQG95_000353"
     CDS             complement(14378..14779)
                     /gene="hslR"
                     /locus_tag="JQG95_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY5887038.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14790..15458)
                     /gene="yrfG"
                     /locus_tag="JQG95_000354"
     CDS             complement(14790..15458)
                     /gene="yrfG"
                     /locus_tag="JQG95_000354"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY5887039.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15523..17658)
                     /gene="igaA"
                     /locus_tag="JQG95_000355"
     CDS             complement(15523..17658)
                     /gene="igaA"
                     /locus_tag="JQG95_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY5887040.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17978..18538
                     /gene="nudE"
                     /locus_tag="JQG95_000356"
     CDS             17978..18538
                     /gene="nudE"
                     /locus_tag="JQG95_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY5887041.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18703..21255)
                     /gene="mrcA"
                     /locus_tag="JQG95_000357"
     CDS             complement(18703..21255)
                     /gene="mrcA"
                     /locus_tag="JQG95_000357"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY5887042.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21375..22154
                     /gene="hofM"
                     /locus_tag="JQG95_000358"
     CDS             21375..22154
                     /gene="hofM"
                     /locus_tag="JQG95_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY5887043.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            22154..22693
                     /locus_tag="JQG95_000359"
     CDS             22154..22693
                     /locus_tag="JQG95_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY5887044.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22677..23117
                     /gene="hofO"
                     /locus_tag="JQG95_000360"
     CDS             22677..23117
                     /gene="hofO"
                     /locus_tag="JQG95_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY5887045.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            23107..23511
                     /gene="hofP"
                     /locus_tag="JQG95_000361"
     CDS             23107..23511
                     /gene="hofP"
                     /locus_tag="JQG95_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY5887046.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23423..24661
                     /gene="hofQ"
                     /locus_tag="JQG95_000362"
     CDS             23423..24661
                     /gene="hofQ"
                     /locus_tag="JQG95_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY5887047.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            25062..25583
                     /gene="aroK"
                     /locus_tag="JQG95_000363"
     CDS             25062..25583
                     /gene="aroK"
                     /locus_tag="JQG95_000363"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY5887048.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25640..26728
                     /gene="aroB"
                     /locus_tag="JQG95_000364"
     CDS             25640..26728
                     /gene="aroB"
                     /locus_tag="JQG95_000364"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY5887049.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26820..28106
                     /gene="damX"
                     /locus_tag="JQG95_000365"
     CDS             26820..28106
                     /gene="damX"
                     /locus_tag="JQG95_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY5887050.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDSAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28213..29049
                     /gene="dam"
                     /locus_tag="JQG95_000366"
     CDS             28213..29049
                     /gene="dam"
                     /locus_tag="JQG95_000366"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY5887051.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            29067..29744
                     /gene="rpe"
                     /locus_tag="JQG95_000367"
     CDS             29067..29744
                     /gene="rpe"
                     /locus_tag="JQG95_000367"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY5887052.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29737..30495
                     /gene="gph"
                     /locus_tag="JQG95_000368"
     CDS             29737..30495
                     /gene="gph"
                     /locus_tag="JQG95_000368"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY5887053.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30488..31492
                     /gene="trpS"
                     /locus_tag="JQG95_000369"
     CDS             30488..31492
                     /gene="trpS"
                     /locus_tag="JQG95_000369"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY5887054.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCVYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31762..32667
                     /locus_tag="JQG95_000370"
     CDS             31762..32667
                     /locus_tag="JQG95_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887055.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32684..33046
                     /locus_tag="JQG95_000371"
     CDS             32684..33046
                     /locus_tag="JQG95_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY5887056.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            33130..34293
                     /locus_tag="JQG95_000372"
     CDS             33130..34293
                     /locus_tag="JQG95_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY5887057.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34294..35520
                     /locus_tag="JQG95_000373"
     CDS             34294..35520
                     /locus_tag="JQG95_000373"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY5887058.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35517..36395
                     /locus_tag="JQG95_000374"
     CDS             35517..36395
                     /locus_tag="JQG95_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY5887059.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36406..36759
                     /locus_tag="JQG95_000375"
     CDS             36406..36759
                     /locus_tag="JQG95_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY5887060.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36771..38075
                     /locus_tag="JQG95_000376"
     CDS             36771..38075
                     /locus_tag="JQG95_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887061.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            38087..39172
                     /locus_tag="JQG95_000377"
     CDS             38087..39172
                     /locus_tag="JQG95_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY5887062.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPGGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(39332..40063)
                     /gene="phoB"
                     /locus_tag="JQG95_000378"
     CDS             complement(39332..40063)
                     /gene="phoB"
                     /locus_tag="JQG95_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY5887063.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(40163..40948)
                     /gene="frlD"
                     /locus_tag="JQG95_000379"
     CDS             complement(40163..40948)
                     /gene="frlD"
                     /locus_tag="JQG95_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY5887064.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40945..41775)
                     /gene="frlC"
                     /locus_tag="JQG95_000380"
     CDS             complement(40945..41775)
                     /gene="frlC"
                     /locus_tag="JQG95_000380"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY5887065.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(41825..42847)
                     /gene="frlB"
                     /locus_tag="JQG95_000381"
     CDS             complement(41825..42847)
                     /gene="frlB"
                     /locus_tag="JQG95_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY5887066.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42868..44256)
                     /gene="frlA"
                     /locus_tag="JQG95_000382"
                     /pseudo
     CDS             complement(42868..44256)
                     /gene="frlA"
                     /locus_tag="JQG95_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324580.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(44500..44667)
                     /locus_tag="JQG95_000383"
     CDS             complement(44500..44667)
                     /locus_tag="JQG95_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY5887067.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(44914..46287)
                     /gene="cobA"
                     /locus_tag="JQG95_000384"
     CDS             complement(44914..46287)
                     /gene="cobA"
                     /locus_tag="JQG95_000384"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY5887068.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(46306..47112)
                     /gene="nirC"
                     /locus_tag="JQG95_000385"
     CDS             complement(46306..47112)
                     /gene="nirC"
                     /locus_tag="JQG95_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY5887069.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(47238..47564)
                     /gene="nirD"
                     /locus_tag="JQG95_000386"
     CDS             complement(47238..47564)
                     /gene="nirD"
                     /locus_tag="JQG95_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY5887070.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(47561..50104)
                     /locus_tag="JQG95_000387"
     CDS             complement(47561..50104)
                     /locus_tag="JQG95_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY5887071.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(50366..51547)
                     /gene="tsgA"
                     /locus_tag="JQG95_000388"
     CDS             complement(50366..51547)
                     /gene="tsgA"
                     /locus_tag="JQG95_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY5887072.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            51818..52399
                     /gene="ppiA"
                     /locus_tag="JQG95_000389"
                     /gene_synonym="rotA"
     CDS             51818..52399
                     /gene="ppiA"
                     /locus_tag="JQG95_000389"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY5887073.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            52504..52671
                     /locus_tag="JQG95_000390"
     CDS             52504..52671
                     /locus_tag="JQG95_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY5887074.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            52661..53263
                     /locus_tag="JQG95_000391"
     CDS             52661..53263
                     /locus_tag="JQG95_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY5887075.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            53295..53858
                     /gene="pabA"
                     /locus_tag="JQG95_000392"
     CDS             53295..53858
                     /gene="pabA"
                     /locus_tag="JQG95_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY5887076.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            53944..55164
                     /gene="argD/dapC"
                     /locus_tag="JQG95_000393"
     CDS             53944..55164
                     /gene="argD/dapC"
                     /locus_tag="JQG95_000393"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY5887077.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(55231..57321)
                     /locus_tag="JQG95_000394"
     CDS             complement(55231..57321)
                     /locus_tag="JQG95_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887078.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(57372..58004)
                     /gene="crp"
                     /locus_tag="JQG95_000395"
     CDS             complement(57372..58004)
                     /gene="crp"
                     /locus_tag="JQG95_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY5887079.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            58306..58710
                     /locus_tag="JQG95_000396"
     CDS             58306..58710
                     /locus_tag="JQG95_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY5887080.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(58765..59634)
                     /locus_tag="JQG95_000397"
     CDS             complement(58765..59634)
                     /locus_tag="JQG95_000397"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY5887081.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(59688..59906)
                     /locus_tag="JQG95_000398"
     CDS             complement(59688..59906)
                     /locus_tag="JQG95_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY5887082.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(59900..60922)
                     /locus_tag="JQG95_000399"
     CDS             complement(59900..60922)
                     /locus_tag="JQG95_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY5887083.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(60922..>61491)
                     /locus_tag="JQG95_000400"
     CDS             complement(60922..>61491)
                     /locus_tag="JQG95_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5887084.1"
                     /translation="PNLLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRS
                     TTDDLYLVHDRKVEPFDGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRRE
                     AELRAQTQPLRKEIARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQAS
                     AKSGLEECEMAWLEAQEQLEQMLLEGQSN"
BASE COUNT        14398 a        15693 c        16636 g        14764 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctactcgc cgcagccaac tcttcttctg
       61 acagcccggt aaatcgcatg atgtctgcaa gagggactcc ggattcaagc attattttgg
      121 ctatatgcag ggctttggat tgttccccct cctgccgcaa tctttccgca atagtcatta
      181 aactctcctt gtgtttcggt gaacgttcgg caacgccgtc gataaaatcg ttaaaacgta
      241 cagcgtcgcc agtttgcagt atgtaattaa acagcccttt gatttgtctg tcattagcgt
      301 atccactact taataagcag gccatttgct ctaccagccc catcagatcg cgttgacgaa
      361 tatgtttttg aattaactcc agcagcgcca tacgtcggtg ctgcatgatt tcatcatcag
      421 gcatgacggt gacatcaatc agcggaaatg cggaggcata aagctgcctt gccagtttgg
      481 gatcggcgaa acaatccagc caacacagcg aatagggata ggggctttca ataccgtggt
      541 aaaacaacaa tggcaccacc atcggcaacg ttttgtatcc agcatcaagg tgattttgca
      601 ttgcggcaat agcgtaacgc atcatgcgaa atgcgatcag tttgtttgag gtgctttgat
      661 gttcaatcag acaatagatg tatcctggtc cttgttccgt tttcaccgac cacagcacat
      721 cggaatagct ttcacgcaga tcgtcatcaa taaagctcct cgactccagc ttgagggttt
      781 tcatatcaca gagcgcgtga atgggtgccg gtaaatgaaa cgcaagaaaa tcgcgtgccg
      841 tctccggttg gcgtaaaaag agtttgaaca gcgcatcatg tggggtggaa ctctgctttt
      901 tactcatggc gttccttcac ctgttgtttg aatgccgcga ctctacgcgc cactccacat
      961 ctgtgcatta gcgagttttc atccctgcga gacgctttca gaggaactta cgttgcgctg
     1021 caagaattgc atttttatgg aaagccgcac gcaaaaaagc ccggatgcaa acgcataccg
     1081 ggcttttcaa aacaggcgat ggagtatggt taacgcagcg cccaccactc gcgcagacag
     1141 gctttgcctt ccgggcagct tttacaactg ccagagaggc agccgtcagg ttcttcctga
     1201 atccgcacgg ctttgcccat gctttccagc tgttgcagca tggcgttaat cattggctgc
     1261 ggagtgttca atgtctggct tatctgggtc gcttccatac ggccccgtaa cgccagcaaa
     1321 tcacgtacct gaataagtga agccattaat ggcaatcacc ggtggtgctg gctgcgcagc
     1381 aactgcttac cgacttgcgg gttgccagca gttcgatatc cacccggcta cgcgcgcggc
     1441 gcagcagacc gatagctacg atgttaaaca ggattaccgc cagaatgcac accaggctgt
     1501 aagttgggtg ctgactgtag ctggcgacct gatagaacaa tgttgccagt gagtaagcga
     1561 tattcagccc ccacaggatg gagaagccca tccagccacg gcttgattca cgggcgatgg
     1621 ctcccatcac cgagatacat ggtacataca gcaggacgaa aatcaggtag ctgtatgcgg
     1681 cagctgcgct accgaatttc tgatccatca cgcccatcgc cccggtaccc atttcaccgt
     1741 tgcctttgct agcttcaatg gggttcatca gtacgctaag gctgaaggtg tctttcaggc
     1801 tctgccaggt ttcatctacc gcactgaaca gctcttcacc gaggttgaat tccgccggat
     1861 tgaactcttc gtcctgaata ttttctgcgg tgtagagggt gttgagcgta cccaccacca
     1921 cttcttttgc cattgcgccc gtaaacaggc caaccgttgc ctgccagtta tcttcatgta
     1981 cgccaatcgg cttgaagaca ggggttatca cccggctgac ggaagccagc gccgagtcgt
     2041 tgatgttatc gacgattttc ccgctcaacg agaagctgtt gaaagcgctc aggaaaatgc
     2101 tgacgatgat aatcacttta ccagcacgca gaacgaagcc cttcagacgc tgccaggtct
     2161 ggataatcag gcttttaacg tgtggtacat ggtagaccgg cagctccatg acaaacggcg
     2221 tcgcttcacc gcgcatgatg gtgtacttga gcatcaggcc agtcagcacc gccatcacga
     2281 taccgagcat atacagcgag aaaaccgcca gggcaccgtt ctgcccgaag aaggcagccg
     2341 cgaatactgc gaagatagcc agacgcgcgc cgcaggacat aaacggtgcc atcatgatgg
     2401 tcatcagacg ttcacgcggt gcatcaagcg tacgtgcgcc cattaccgac ggtacgttac
     2461 aaccgaaacc gacgatcagc ggcacaaagg atttccccgg caagcccagc gcctgcatca
     2521 ggcggtccat cacaaacgct gcacgcgcca tatagccgga gtcctcaagg aaggagagga
     2581 acaggtacat catgccgatc tgcggcacca gtggcagcac ggtgttaata ccgccaccca
     2641 ggccctgggc gaggaagata gtcagccagt ccgggaagtg gagcgtgtag ccaatccatt
     2701 gaataccatg cacaaatagc gccacggagc cgacgttaaa cagcggctgt aacgccccgc
     2761 cgatgttgat agccagcagg aacatcaggt acatcacaaa gaggaaaatc ggcagaccga
     2821 ggaaacggtt gagcacgatt ttatctaccg cagtggtgaa acggctgggt tctgccgtca
     2881 gggtgttgct taccacatca cagatggcag caatgcactg gtaacgcgca tcggcaatgt
     2941 gcagcgccgg atcatccatt tcattacgca gacgggcgag ggtggcatcc agatgctgcg
     3001 acgcttcacc ggcgtaggcg cggctgtaga tatcgccttc cagcatttgc aggcccagcc
     3061 agcgacgttg tttcagcggg atgtcggaag gcatcacttt tgccagtgaa tctgcttcgt
     3121 tgagtagcgg ctgtgcgtaa tgcaccagtt ccacattctc gttagcttta tagcgatcaa
     3181 tcgccagctt gagcgcttca ataccgcgac cacgggttga aaccagcggg atcaccgggc
     3241 agccaagacg tgccgataga gcatcaattt caatacgaat attttgcttc tcggcaatgt
     3301 cgagcatgtt cagcgccacg atgcagggaa tgccgagttc cagcagttgc agcgtcaggt
     3361 acaggttacg ctcaaggtta gacgcatcca ccacgttaat cagcaggtcg gcgtcgccac
     3421 tcaaaatgta gtgacaggcg atttgctcat cgagcgaggt ctgcgatgag atggtggtca
     3481 gagaataggt gccgggcagg tccaccagcg tgacctgatg atcggtggtg gagaattgcc
     3541 cttctttacg ttcgacggta acgccagccc agttacctac acgctgacgt gcgccagtga
     3601 gctggttaaa taaggttgtc ttgccagaat ttggattacc aattaagcca atggttaatt
     3661 ttttcattgt tgttatcacc gtattaacag gaaaccgctt ccacttctaa taaggccaga
     3721 tcttttttgc gtaataccag gctcacacga cgggtttcga tatgaatggg gtcgccgagt
     3781 ggagcgacgc gcaccacatc aaaagaggag ccaggtaaca tgcctagaga aagcagtttt
     3841 tggcgatatg ccgggctgat ttcacgggaa aagccagtga ttttccacgc agtatctgga
     3901 gtgtattgca taggtgccta cttgtttctc attaactgga taactacctc atggggcgtt
     3961 tgaccgcgac atgagaccag atgatttgcc aacaaaggag agattaaata atcaacaaca
     4021 caatgataat gagaatggtt tctatcagca atacttaaaa tgtgtgcgaa tgttggtttg
     4081 aataataatc tgactcaatg ttgatgtggc tcaaggttat atcgcagcga aaatataatt
     4141 cgaatagtga gttacccgga ctggctaatg tttaattaag gtttcagttg ttaatgaagt
     4201 gataaatatt aacggagtat attgtgagtt tggctgtgga ataaaagtgc cggagatatt
     4261 ctccggcact tttatgtcag gcaaagcgtt cgttgccaga tgcggcgtga acgccttatc
     4321 gcgcaggttt aggggccagt ccgggcgttt aacgtttttt acccatcgct gccgccagcg
     4381 catccatcat cgcgctatta ccggcaggct gcgcttcacg accgcgtggt ttggctgccg
     4441 ggcggttgtt ttgcgggcgt tcattaccgc cgccgcgacg agagttggtt tcgccaggct
     4501 gctcgtccag acgcatagtc agggcgatac gtttacgctg aagatccact tccagcactt
     4561 tcaccttcac aatgtcgccc gctttcacca cggtatgcgg atcttccaca aacttgttcg
     4621 ataatgaaga gatgtgaacc aggccgtcct gatgcacgcc aatatcgaca aacgcgccaa
     4681 agttggtgac gttggtcact gcgccttcga ggatcatacc cggttgcagg tcgttcattg
     4741 tctcgacgcc atcggcaaac tgagcggttt taaattccgg acgcggatcg cgacccggtt
     4801 tttccagctc tttgatgatg tcagttactg tcggcacacc gaatttttca tcagtaaagt
     4861 cagacgcttt caggttacgc agttcgctgc tgttacccat cagatctttc agtgcctgct
     4921 gtgttgctgc cagaatgcgt tccaccaccg gataggcttc cggatgaacg gtagaggcgt
     4981 ccagcgggtt atcaccgtgg ttaatgcgca ggaagcccgc gcactgctcg aaggctttcg
     5041 gccccagacg gctgactttc agcagttgct gacggttctg gaactggccg ttctcatcgc
     5101 gccaggccac gatgttttgc gccatcatgc gcgtcaggcc cgccacgcgg gttaatagcg
     5161 gaacagaagc ggtgttgaga tcgacgccaa cggcgtttac gcagtcttct actactgcat
     5221 ccagtttgcg ggccagttgc gtctggctga cgtcatgctg atactgacct acgccgatag
     5281 atttcggatc gattttcacc agctccgcca gcggatcctg caaacggcgg gcgatagata
     5341 ccgcaccacg cagcgaaacg tcgagatccg ggaactcctg cgctgccagc tcggaagctg
     5401 agtaaaccga cgcgccagtt tcgctgacga tcactttctg tgcggtcact ttcgggaact
     5461 gcttctgcac gtcgagatag aaacgttcag tttcgcggga agctgtaccg ttgccgatcg
     5521 ccaccagttc aacgttatgt ttttcacaca aggcagcaac ggtcatcgct gcttttgcgg
     5581 cttgtccggt gtgcgggtaa atggtatcgg tcgccaccag tttgccagtg gcatcgacca
     5641 ccgccacttt taccccggta cgcagacccg gatcgaggcc catcgttgca cgcagtccgg
     5701 caggggccgc catcagcaga tcgtgcaggt tacgggcaaa gacgttgatt gcttcatctt
     5761 ccgcgcgttc gcgcacggta cccatcagtt cggtttccag atgcatcagc accttgatgc
     5821 gccaggtcca gctcactacg cttttgcgcc agctatccgc cggggcattg ttcaggcgca
     5881 ggccaaggtg atccatgatg atttgctcgc aatagctctc tttgggcggc tcatcgaact
     5941 gcggatcggc attcagcgaa agctggagta cgccttcgtt acgcccacgg aacatcgcca
     6001 gcgcgcggtg agaaggcacc gtggacaacg gttcgtgatg atcgaaatag tcgcggaatt
     6061 tcgccccttc ctcttcttta ccgctcacca ccgtagaaac caaatgcgcg ttcttccaca
     6121 gataatcacg cactttcgcc agcagcgcgg catcttcggc aaaccgttcc atcaggatat
     6181 agcgcgcgcc atccagcgcg gctttggtat ctgccacgcc tttatcggca tcaacatatt
     6241 gtgcagcggc gacttctggc gtgtgtgacg gatcgctcca cagcaggtca gccaacggct
     6301 caagccctgc ttcaatggcg atttgcccgc gggtgcggcg tttaggtttg tagggcaggt
     6361 agaggtcttc gagttcggtt ttgcttaggg tggcgttgat ggccttcgcc agatcatcgg
     6421 tgagtttgcc ttgctcggaa atggatttga ggatcgcctg acgtctctct tccagctcgc
     6481 gcagatagct cagacgcgtt tccagattac gcagctgcgt gtcatccaga ccgccggtga
     6541 tttccttacg ataacgtgcg ataaacggca cggtattccc ttcgtcaagc aggcgaacgg
     6601 cagcgtcaac ctgttccggg cgcgcctgaa tttcacccgc aataatgcgg cagaacgaat
     6661 cattcatcat ggtttgagtt catcttttcg gatcaaaaat caggggatag ttatacggac
     6721 tggctggcaa aaatgccagc catcggcagg aggttaagac tcttccttac ggtttcacgt
     6781 actcgatagc attaacatac cagttcgctt ccccggcagg ggtattcacc accgccagat
     6841 cgccgacttc ttttttcagc aatgcgcggg ccatcgggga atcgatagag atgtaatctt
     6901 tacggccaaa aatttcatcg tagccgacaa tacggaaacg gtgagtcacg ccatcgtcgt
     6961 tttcaatctc cacccacgcg ccaaaaaata ctttgccttc ctgctgaggg gagtaatcga
     7021 cgattttgag attttccagg catttagtga gatagcgcac gcgacggtcg atttcacgca
     7081 gacgcttttt attatactga tagtcagcat tttcgctgcg gtcgcccaga cttgcggccc
     7141 aggtcacctt ttttgtgacc tccgggcgtt cttcacgcca gagataatta agctcttgtt
     7201 tgagtttttc atacccttcc cgggtaacca ggggcgtttt catctcgttg attccctttg
     7261 tctgtttgat aatgcgcaca ttgggtataa cgtgatcata tcaacagaat caataatgtt
     7321 tcgccgaata aattgtatac ttaagctgct gtttaatatg ctttgtaaca atttaggctg
     7381 aaatttatac cagatttagc tggtgacgaa cgtgagcttt tttaagaata cacgcttaca
     7441 aattgttgcg aacctttggg agtacaaaca atgcaagaga actacaagat tctggtggtc
     7501 gatgacgaca tgcgcctgcg tgcgctgctg gaacgttatc tcaccgaaca aggcttccag
     7561 gttcgaagcg tcgctaatgc agaacagatg gatcgcctgc tgactcgtga atctttccat
     7621 cttatggtac tggatttaat gttacctggt gaagatggct tgtcgatttg ccgacgtctt
     7681 cgtagtcaga gcaacccgat gccgatcatt atggtgacgg cgaaagggga agaagtggac
     7741 cgtatcgtag gcctggagat tggcgctgac gactacattc caaaaccgtt taacccgcgt
     7801 gaactgctgg cccgtattcg tgcggtgctg cgtcgtcagg cgaacgaact gccaggcgca
     7861 ccgtcacagg aagaggcggt aattgctttc ggtaagttca aacttaacct cggtacgcgc
     7921 gaaatgttcc gcgaagatga gccgatgccg ctcaccagcg gtgagtttgc ggtactgaag
     7981 gcactggtca gccatccgcg tgagccgctc tcccgcgata agctgatgaa ccttgcccgt
     8041 ggtcgtgaat attccgcaat ggaacgctcc atcgacgtgc agatttcgcg tctgcgccgc
     8101 atggtagaag aagatccagc gcatccgcgt tacattcaga ccgtttgggg cctgggctac
     8161 gtctttgtac cggacggctc taaagcatga ggcgattgcg cttctcgcca cgaagttcat
     8221 ttgcccgtac gttattgctc atcgtcacct tgctgttcgc cagcctggtg acgacttatc
     8281 tggtggtgct gaacttcgcg attttgccga gcctccagca gtttaataaa gtcctcgcgt
     8341 acgaagtgcg tatgttgatg accgacaaac tgcaactgga ggacggcacg cagttggttg
     8401 tgcctcccgc tttccgtcgg gagatctacc gtgagctggg gatctctctc tactccaacg
     8461 aggctgccga agaggcaggt ctgcgttggg cgcaacacta tgaattctta agccatcaga
     8521 tggcacagca actgggcggc ccgacggaag tgcgcgttga ggtcaacaaa agttcgcctg
     8581 tcgtctggct gaaaacctgg ctgtcgccca atatctgggt acgcgtgccg ctgaccgaaa
     8641 ttcatcaggg cgatttctct ccgctgttcc gctatacgct ggcgattatg ctattggcga
     8701 taggcggggc gtggctgttt attcgtatcc agaaccgacc gttggtcgat ctcgaacacg
     8761 cagccttgca ggttggtaaa gggattattc cgccgccgct gcgtgaatat ggcgcttcgg
     8821 aggtgcgttc cgttacccgt gcctttaacc atatggcggc tggtgttaag caactggcgg
     8881 atgaccgcac gctgctgatg gcgggggtaa gtcacgactt gcgcacgccg ctgacgcgta
     8941 ttcgcctggc gactgagatg atgagcgagc aggatggcta tctggcagaa tcgatcaata
     9001 aagatatcga agagtgcaac gccatcattg agcagtttat cgactacctg cgcaccgggc
     9061 aggagatgcc gatggaaatg gcggatctta atgcagtgct cggtgaggtg attgctgccg
     9121 aaagtggcta tgagcgggaa attgaaaccg cgctttaccc cggcagcatt gaagtgaaaa
     9181 tgcacccgct gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc gcccgttatg
     9241 gcaatggctg gatcaaagtc agcagcggaa cggagccgaa tcgcgcctgg ttccaggtgg
     9301 aagatgacgg tccgggaatt gcgccggaac aacgtaagca cctgttccag ccgtttgtcc
     9361 gcggcgacag tgcgcgcacc attagcggca cgggattagg gctggcaatt gtgcagcgta
     9421 tcgtggataa ccataacggg atgctggagc ttggcaccag cgagcggggc gggctttcca
     9481 ttcgcgcctg gctgccagtg ccggtaacgc gggcgcaggg cacgacaaaa gaagggtaaa
     9541 taaacgggag gcgaaggtgc ctcccgtttt gctttctata agatactgga tagatattct
     9601 ccagcttcaa atcattacag tttcggacca gccgctacca gcgcggcacc cgcaggggtg
     9661 tcggtgtatt tatcgaagtt gtcgataaac agtttcgcca gggtttcggc tttttcctgc
     9721 cactgttccg gagaagcgta ggtgttacgc ggatcgagaa tcttcgtgtc tacgcccggc
     9781 agttcggttg ggatcgccag gttaaacatc ggcagagtga aggtttctgc attatccagc
     9841 gaaccgttga ggatggcgtc gataatggcg cgggtatctt taatcgagat acgtttgcca
     9901 gtgccgttcc agccagtgtt aaccagataa gcctgcgcgc ccgccgcctg catacgtttc
     9961 accagcactt ctgcgtactg agtcgggtgc agcgacagga acgccgcgcc gaagcaagcg
    10021 gagaaggttg gcgtcggttc ggtgatgcca cgctcagtac cggccagttt ggcggtgaag
    10081 ccagagagga agtgatactg ggtttgatcg gcagtcaggc gagaaaccgg cggcaacacg
    10141 ccgaaagcat cagcagtcag gaagataacc ttagtcgcgt ggcccgcttt ggaaaccggc
    10201 ttaacgatgt tatcgatgtg atagattgga taagaaacgc gggtgttctc ggtttttgaa
    10261 ccatcatcaa agtcgatagt gccatcttca cgcacggtga cgttttccag cagcgcatca
    10321 cgacggatag cgttgtagat ttcaggttcc gcttctttcg acagcttgat agtttttgcg
    10381 tagcagccgc cttcgaaatt aaacacgccg tcatcgtccc agccgtgttc gtcatcgcca
    10441 atcaggcggc gtttcgggtc ggtggaaagg gtggttttac cggtgccgga aaggccgaag
    10501 aacaccgcaa catcgccttt ctcgccaacg ttggcggagc agtgcataga agcgatacct
    10561 ttcagcggca gcaggtagtt catcatcgag aacatccctt tcttcatttc gccgccgtac
    10621 caggtgccgc caatcagctg catgcgctcg gtcaggttaa acgccacgaa gttttcggag
    10681 ttcagaccct gttctttcca ctgcgggtta gtgcacttcg cgccgttcat aacgataaag
    10741 tctggtttga aacctgccag ctcttcatcg ctcgggcgaa taaacatgtt tttgacaaaa
    10801 tgcgcctgcc aggccacttc ggtgatgaaa cggacggaaa gacgagtatc cgggttcgca
    10861 ccacagaaag cgtcgacaac gaacagacgt ttgccggaaa gctgcctggt caccaggcct
    10921 ttcagatgct gccaggtttc cggagagaga ggtttgttgt cgttcttacc tttgcctttg
    10981 tctgcccacc agaaagtatc gcgagtggta tcgtcacgga cgatatactt atcttttggt
    11041 gaacgaccgg tgaagatccc ggtatcgacg gcaacggcac ccagattagt taacaccccg
    11101 cgctcataac ctgtcaggct cggatcgagc tcttcctgat acagcaggtc gtagcttggg
    11161 ttgtaaacga tatcatgtac gtcactgata ccataagcct cgagttcttg cgggggcaaa
    11221 ccattgttaa cgcgcatttc actgctcctt agccaatatg tattgcctga atagtaaagt
    11281 ctttttgggg gtgttaaccg cgacaaggct catagattta cgtatctgga gaaattcata
    11341 tttgacaaaa aactccgtga ttcctgtcac gaaacggttg ctattatcgc agaaatagca
    11401 ttttaggtgt ggaaaatgtt ctcaaaaggt taaatcttgg tttatatggc gggaatattg
    11461 tgagtggaat cgcattcctg aatgaaattg atgcaaattc aaccggtaaa gtatcgattc
    11521 accacgctaa ctttttttga ttattttctg aaaaaagcaa aatactactg cccggatgcg
    11581 ataattaacc caccttttga ttctggataa ccaacatgga taacgttgaa ctttcaccgg
    11641 caacacgctg ggggatgatt gctaccggat tacttcaggg gctggtttgc tacctgctta
    11701 tcgcctggct tgccggaaaa aatcacagtt ggattgttta tggcgtgcct gcgaccgtcg
    11761 cgttttcatc cgttttactc ttctccgtga tctcttttaa acagaagcgc ctttggggat
    11821 ggctggcgct ggtgtttatt gccacgacag ggatgagcgg ttggctgaag tggcaaactg
    11881 atggcatgaa cccctggaga gctgaaaagg cgatttggga ttttggctgc tatctgctgt
    11941 tgatggcaat gttgttgcta ccgtggatac aacaaagcct tcgtatccgc aatggcagta
    12001 gccgctatag ctacttttac cagtcagtat ggcataacgt acttatatta ctggtgattt
    12061 ttctctccaa tggcctgacg tggctggtgc ttttactgtg gggtgagttg tttaaacttg
    12121 ttggcatcac gtttttcaat acgctctttt ttgcaaccga ctggtttatg tatcttacat
    12181 ttggtctggt taccgcactg gcggtgatcc tcgcgcgtac acagtcacgt ttaatcgact
    12241 ctattcaaaa gttgttcacg ctaatcgcca cggggttgct gccgttagta tcattgctaa
    12301 ccccgatgtt tatcatcacc ctgccgttta cgggcctgag cgcgatttct cgccacatct
    12361 ccgccgccgg gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc
    12421 gcgatccgca aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg
    12481 ctttgctggt tgctccgctg tatgtgttcg tcgccacctg ggggttatgg ctgcgggtcg
    12541 ctcagtacgg ctggactgtc gaccgcttgc agggcgcgct ggcggagctg gtgttactgg
    12601 tgtggtcgct ggggtatttt gtcagtatcg tctggcgtaa tgggcaaaat ccccttgttc
    12661 ttcagggcaa agtgaacctt gcggtttcgt tattggtgtt ggtgatactg gtgcttctta
    12721 attcgccggt gctggacagt atgcgtatta gcgtgaacag tcatatggcg cgttatcaga
    12781 gcggcaaaaa cacgccagac caggtaacta tctacatgct cgaacagagc ggtcgctatg
    12841 gacgtgcggc gcttgagtcg ctgaaaagcg atgccgggtt tatgaaagac ccgaaacgcg
    12901 cgcgggatct gctgatggcg ttagatggag agcaacatct tcaggaacag gtatcggaaa
    12961 aagtattcgc cgaaaatgtg ttaattgccc ctggttctgt taaaccggat gcgacattct
    13021 ggtcggcctt aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg
    13081 tactggtcga gcaagatctg aatagtgatg gtcaggcgga gcggatcctg tttgctttta
    13141 atgatgacag agtcattgtc tatggctttg actcagacag aaaagaatgg gacgcgcttg
    13201 atatgagttt acttccgaac gaaataacga aagaaaaatt actcacagct gcgaaggatg
    13261 gaaaactggg gacgaggccg aaagcgtggc gcgatctgac ggtagatggt gaaacgctgg
    13321 aaataaactt aagtaagtga caacgctttt atcggatacg acgctgtatc tgaccaaaat
    13381 aatttgcgat ttatttcata ggcgctggta agcgcagcac atcaggccat taagtcgtaa
    13441 aatccggcag agccctctct gccggacata ctcattaatg aacttacgga tctgccggag
    13501 acgcgttgtt gcggatttca gcaatatcca tcgcattgaa cagatagtgg ttaccgcagt
    13561 aatcacaatg catgtcaatt tcgccatctt ccgccaggat gctatcaact tcttcatcag
    13621 gcagcgtttt cagcgcatcg gcgcaacgtt cacgcgagca ggtgcatttg aactccacat
    13681 cctgcggatc gtaaaccgtc acctcttctt cgtgatacaa acgccacaac acttcgtttg
    13741 ccggtaaggt cagcagttct tcggttttga tggtttcggt tagcgtcgcc aggtggtcaa
    13801 agtcgtcctg ttgggtattt tgcgcaggca ttacctgcaa caacataccg cctgcagccg
    13861 gtttgccgtc tacgtcgccg gtgcgaataa acaggcgcgt cggcagctgt tcagaacgca
    13921 taaagtaatc ttctaggcag gccgccaggg tatcaccttc cagaccaact acgccctgat
    13981 agcgttcgcc ttcgctcggg gtaatggtga tcaccacgta accattgccg accagcgttt
    14041 tcaggtcggc attttctgga atttcgccct gcacgcgcgc cacaccgcgc atttgctggt
    14101 tattgttacc gttaataacc gccagattca tcggaccgtc gccctgcagc tgtacggtga
    14161 tatcaccatc aaacttcagc gtagcggtta acaggctggt cgcaaccagc agttctgcca
    14221 gcacgttttt aacgggctgc ggataatcgt ggttctcaag gatctgttgc agggtttccg
    14281 aaacggttac cagttcgccg cgaacggcaa aattttcaaa cagatagcga tgtaattggt
    14341 catgttgcgg cataatcatc tctcttgcag gtgacagtta ttcactgtcg ccgtgtttaa
    14401 atcgtaacag gtcgcggcgc tcttttttgt ccgggcgtcg gtccgggtgc ggcatggtta
    14461 aggcattaag tttacgtgcc atcgccattt tttcgcgttt ctctacactt tccgcagtct
    14521 cttcatacag caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa
    14581 tcaccgtgcg ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt
    14641 tgctcggctt gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg
    14701 ccagcgcgcg ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa
    14761 cagcaggttt ctctttcatg gcgtctcctt cacattagcg aggggatcag gcggcggtag
    14821 tcattcagtg acggatggcg ttgatactgt ttctcggcaa tcccggaatc aggattagtc
    14881 acgccgaggc agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca
    14941 tcaataaaca gcgttctttc agctttcaga cccgtagctt cggccaccgc atgccataac
    15001 cgctgatcct ctttcggata accaaatgtg tgggtggaaa gtaataaatc aaggtgtgcg
    15061 tccagacccg tatgctcaag ttttaccgcc aggttgtgcg gatgagcatt ggtgagcaaa
    15121 attcgctgct taccgctggc tttcagtgcc tcaagaaacg gaatggtatc ttcacgcagt
    15181 acggcgcgcg gtcccatctc ggtggtcatc gcacagatat ccagacccag ttgctcactc
    15241 cagtaatcaa gacagtacca gtttagcgta tgctgtacgt cgtgatattg ctggcgcata
    15301 tattccatcg cttcctgtgg cgtaaccccg tttttcgcgc cccatgtttc aggcaccagc
    15361 ttttgccaga aatagttatc gaaggcgagg tcgagcaacg tgccgtccat atccagcaga
    15421 acggtatcta cgtcctgcca ggcaatgttg atatgcatga gggaaatctc cagagtgaag
    15481 caatttgcgc gacagggtag cataacctgc cgcgcaaacg tgttattcga taaggctttc
    15541 tgaaggggtg atcagttgcg ggttcaggca gctttcataa taggcctgaa tctccatcat
    15601 gcgagtgcga tgacgctggt aacgacgcca ggcctgtacg ccattgtaaa tggcgctacc
    15661 cagcatcgtc agtagcagca atgtggtgct gagatagcgc cacaggccgg aacgatccgg
    15721 gatcggatga aggccaatat gctgcgtacc attggcgtca gtgaagattt ttgtgacgat
    15781 accttcggcg ttaaacggcg tatgcatcag catttgtgcc agtttctgga aagcgttcca
    15841 ctgttcttgc ggcgggtagt cgtaaagtga tgccgaaggc cagggctgat caacaaaatc
    15901 gctgccttcg tcgctgacaa tcaggaatcc gccgggggcc ggactgttta gtgattgtgc
    15961 cgctcgcgcc gtttcatgcg tgataaacgg cgcggtggag gttgccacca ggttatccag
    16021 cgattccgca ctcaccgggc gtaataacac attcacgcca tcgagcttcc cggcgttggc
    16081 gcgttttacc agcgcgtccc agtctttact gttgccgaga ttgaccagcg catttttcag
    16141 tcgcacacag tcatctttgg cagaacataa atccgctgtc ttcagtacaa tgtcgccaaa
    16201 atcatcaagc aataccatgc cggatttttg aatcgctgaa cgcaatgagg cactgacgcg
    16261 agattcatct tccggtttag ggtgcagctg gcgattaact gcttcagtca atgccgtcgc
    16321 tttgttgacc agttcagatt ctggtaatgg taatgagcgg gcgtcattcc agatgatctg
    16381 cgagcagtca aacggtaaaa aaggtgaatt ggttttcgcg ctccaggttc cggaagtgcg
    16441 aatattacac attcccgtac cgctaatacg caatgtatcg cctacccgca cgccagcgtc
    16501 agccagttgt tttacgctgg tggcttcaat ggtctgcgcg cctttcatcc atgagagagt
    16561 gaatttcagc ggcatatcca gcgggatcca gaataacagc ataaacagca ccagcagcga
    16621 gcccgcagcg ataatcgtac tgcgcagcca gtgctgtaac ggaaagtttt ttacttcatc
    16681 atgcagcgaa agatatcgcc cctgacgcac tacgtggcgg tcgagataga tatcaatatc
    16741 ggtttgttga ccgagatcct gagcaatgta tggctgccag tgtgcgggat agaccaggtc
    16801 gataataccg agtgaaatat tgttgatctg ttcctgatcg ttttcgccaa acaatcccca
    16861 acgacggggt gtaccgcgca gacaatgaat ttctcgcagg gaggattttg ccgggggcgc
    16921 gaatagcccc cacagacctg cgcccagcag cagtaatgcg ccgcctgcca gccacggaac
    16981 aaatacatcc ggggtaatca ggcagaaaaa gaacatcagg aaggaggcga cgatcagcaa
    17041 cgcttcacgc agcccgcgcg gacgactcag cgcatattct tcatgcgttt ctttgcgaat
    17101 attgagtagc tcgatttgct cactctcttc cccgcgaatc gacgcctgcg tggacggaac
    17161 aggttgcaag gcatagccgc gcgtttcctg catgtactcc tgcagcgtat gaccgttgag
    17221 tgaaataacc aacggcagcg aatcggtatg aatcagttca acggtattct catcgttgat
    17281 gtactgttcc cagaaggggg gcaggtggac ttctaccgaa tcgaggtagt aacgccattt
    17341 attcggatcg tcggtagaga tgccgtaacg tgtgatagcg tgtgtcagca tcatgacgtt
    17401 gttgctttcg gcattcagcg ccagagagat cggtgccgcg ctagctcccg ttgggccagg
    17461 cacctgtaat acctgcgtca ggctctcaag ataattttca acggcgctac gttcttcagg
    17521 tgtgagttta cgcgtttgcg catccgcgaa ggcgttggtc cagggcagct gacgccgtct
    17581 ggatcgcact tttatcagcc atcccgcaag tagtgagcag gccagcaaag cagctaaaaa
    17641 aatcacaatg gtgctcatgc tttccccatc ttacttagtc tgtcaggcgt ggtcagtcgc
    17701 accggacaca atacatgatt ctgcggttgg ctatcggcaa gcgtggagct taacttgagc
    17761 atcattaagc acatcacagt aaccgctgat catagcaaac cctctgaagg cggaatatca
    17821 gcaaattcct ggagttattt caacctattg acttttaatt tgatttgagg acgattattt
    17881 attaagttac ataaatgtaa ataacatgca attcacattg ccgaaagcgt gtccgatatc
    17941 gcacaataac cgctgatttc acagcataga tcccacgatg aacaaatcat tacaaaaacc
    18001 caccattctg aatgttgaaa ctgtagcccg ttcccgactg tttaccgtcg agagcgtgga
    18061 tctggagttc agcaatggcg tgcggcgtgt ttatgaacga atgcgtccaa ccaaccggga
    18121 agcagtgatg attgtgccga ttgtggatga tcacctgatc ctgatccgcg aatacgcagt
    18181 gggaactgaa tcctacgaat taggtttttc gaaaggatta attgatccgg gtgaaagcgt
    18241 ctacgaagcc gctaaccgcg agctaaaaga agaggttgga tttggtgcga acgatctgac
    18301 ttttttgaag aagctcagca tggcaccgtc ttacttttcc agcaaaatga atatcgtggt
    18361 cgcgcaagat ctctacccgg aatcgctgga aggcgatgag ccagagccgc taccacaggt
    18421 gcgctggccg ctggcgcata tgatggattt gctggaagac cctgacttca acgaagcgcg
    18481 taatgtcagc gcgctgttcc tcgtgcgcga atggttgaaa gggcaggggc gagtgtaaat
    18541 gcgtgaagtt tgcctgatgc acaacgctta tcaggcttat ggggttcctg caatatattg
    18601 atttttcacg attttgttag ccggataacg cgttcacccg catccggcat aaacaaatgc
    18661 actttgtcag ccaactgaaa aggcgccgaa gcgccttttt aatcagaaca attcctgtgc
    18721 ctcaccatta tcgataatgg tcgttcccac ctcgtgcacc gcctgttgtg tcggctgcgt
    18781 accttcgatg aaatactctt cgcggctgtt gccaccatta gctaactgcc cggtgctgcg
    18841 atcgatattc accgtcacaa tacccggagg cggcgtcagc ggctgctccg gcacaccttc
    18901 aagaacggct ttcatataag cgtcccatgc aggctgggca ctcttggcac cgccttcgta
    18961 acctgagatc tgatctttaa tcgctccgga agccgttgta tgaccgagat tacgacggtg
    19021 atcatcaaag ccaatccaga ccgaggtcac aacgcccgga ccgtaacccg agaaccacgc
    19081 atctttcgaa ctgttagtgg taccggtttt cccgccgata tcgcgacgct gcaaatcacg
    19141 acctgcacgc cagccggtac cctgccagcc tggttcacca aagatattgg tgttcaaagc
    19201 actcttaatc aggaatgcca gcggagtgtt gatgacgtgc ggtgcgtact cctgcgcgcc
    19261 agtcttcgcc actaacgcct gatttgcctg ctccagctgc ggcattggta cagaaacatt
    19321 ctgctgctcg cgggagatag cgacatcttc aacatcgtta ttttccagca cgttcgattt
    19381 ctgcgtatca ccgtaaatca ctggaatatc gcattccggg caggctactt tcggtttcgc
    19441 ttcgaaaatc acgccgccct gatcgttttc aattttgctg ataaaccacg ggtccactag
    19501 gaagccgccg ttcgccatga ccgcgtagcc gcgcgccacc tgcattgggg tgaaggacgc
    19561 tgaacccagc gccagcgatt cggtgtggac aatgttttgc gccgggaagc cgaagcgttg
    19621 cagatattct gcagcgtagt cgacgcccat cgcccgcatt gcacgtacca tcaccacgtt
    19681 tttcgactga cccagcccct gacgtaagcg aattggacca gcatactgcg gtggtgagtt
    19741 cttcggctgc cagtcagaac cggcacctgc atcccagcga gaaattggca catcgttcaa
    19801 catacttgcc agcgtcagac ctttatccat cgccgcggtg tagaggaacg gtttgatgtt
    19861 ggaacccacc tgacgcagtg cctgggtggc gcggttaaac ttgctctgat tgaaatcaaa
    19921 gccaccgacc agcgccataa cggcaccatt ttgcggattg atcgacacca gcgccgagtt
    19981 cacttccggc acttgtgcca gccaccatgc atcgccaacc tgacgaaccc agatttgctg
    20041 acccgtttgc agaacatcgg tcactttacg cggcgtcggt ccctgctgag tatccgaacg
    20101 gtaaggacgc gcccagcgaa cgccttccat actcaatgcg acggtcgacc cgtccgccag
    20161 catcgccgtc gctccctgag gattggcgct ggtgactgcg gcaggcagca gcggaccata
    20221 ggtcggcagc gccttcagcg tatcggtaat cttgttgtta tcccacgccg actcgcccac
    20281 tttccacagc acatttgccg ggccgcgata gccgtggcgc atgtcgtagt ccagcacgtt
    20341 attacgtacc gcctgctgcg cggcctgctg cactttgcgg gtgatggtgg tgtaaatgcg
    20401 ataaccgtct tcataggcac tttcgccata acggttatac atctcctggc gcaccatttc
    20461 gctcaggtac ggcgcagaga aagcaatctc cggcgcgtga taattagcgt taatcgcctc
    20521 agtgcgtgtc tgatcgaact gttgctgggt gatataccct tcatccagca tccgcgacag
    20581 tacgacgtta cgccgcgcga cggcacgatc cattgagtag agcggattaa aggtggaagg
    20641 cgctttcggc aacccggcta tcaccgccat ttcgttcagc gtcagttggt cgaccgtttt
    20701 accgaaatag acttgtgccg cagcaccgac accataggcg cggtaaccaa ggtaaatctt
    20761 gttcagataa agctcgagga tttcgtcttt cgtcagcaac tgttcaatgc gaatcgcgag
    20821 gaagacttcc ttaatcttac gcatcatcgt gcgttctgga ctgaggaaga aatttctcgc
    20881 cagctgctgg gtaatggtac tcgccccttg tgacgcgtgg ccggagaaca gcgccacact
    20941 tgccgcacgg aagatcccca ccgggtcaac tccgtgatgc tcgtagaagc ggctgtcttc
    21001 cgtcgcgata aaggctttca ccatctccgg tgggatttga tccaacgtaa ccggaatacg
    21061 acgtttctca ccgtattgag caatcagctc gccatcggcg ctgtaaatct gcatcggaat
    21121 ttgcaggcga acatctttta atgtcaccac atccggcagt tgtggctcga tgtagcggta
    21181 taggccataa atcgagcctg ctcccagcag aatgcaacag actgcaagga tcaaaaaata
    21241 ctttacgaac ttcactggaa atttcccatt tagtttcatt tgggcagttt ataaacaaac
    21301 gcgcggtagt ataaaggcaa gccagacgca ttgatatacc cgtcagagtg acgggtgata
    21361 aggagatcat cacaatgaca tttaagatct ggcaaattgg tttgcattta caacagcaag
    21421 aagcggcagc ggttgcgatc gtgcggggcg caaaagaatg ctttttgcaa cgctggtggc
    21481 ggttgccgct ggcgcacgac attatcaaag atgggcggat tgttgatgcg cagcagctgg
    21541 ctaaaacgtt gttaccgtgg agtcgcgaac tgccgcagcg tcatcacatt atgttggcgt
    21601 ttcccgccag tcgcacatta cagcggtcat ttccgcgccc gtcgatgtcc cttggtgagc
    21661 gggagcaaac ggcctggctg tcagggacga tggcccgcga gctggatatg gatccggact
    21721 ccctgcgctt cgattacagc gaagactcac tcagccccgc ttataacgtg actgccgcgc
    21781 aaagcaaaga gctggcaacg ctgcttacgc tggcagaaag gttgcgtgtt catgttagtg
    21841 cgatcacccc ggatgccagt gcattacagc gattcctgcc ttttttacct tctcatcagc
    21901 aatgtctggc ctggcgtgat aacgaacagt ggctgtgggc gacgcgctat agctgggggc
    21961 gcaaactggc ggtggggatg actagcgcga aggagctggc ggcagcgtta tccgttgatc
    22021 ccgacagcgt cgcgatatgt ggcgaaggcg gatttgaccc ctgggaggcc gtttctgttc
    22081 gtcagccgcc gctaccgccg cctggtggag actttgccat cgcgctgggg ctggcgcttg
    22141 ggaaggcgta ctgatgaacc cgccaattaa ttttttgccc tggcgacagc aacgccggac
    22201 cgcttttctg cgtttctggt tgctgatgtt cgttgcgcct ctgctgctgg ccgtcgggat
    22261 aacgctaata ctgcgtctga caggcagcgc cgaagcgcgc atagacgcag ttttgcttca
    22321 ggcggaacaa caactcgccc gcagcttaca gataacgaag ccacgtttgc tggagcaaca
    22381 gcagttacgc gaacagcgtt cgcaacggca gcgccagcga caatttaccc gcgactggca
    22441 atctgcgctg gaagcactgg cggcgctttt acccgagcac gcctggctga caacgataag
    22501 ctggcagcag ggaacgctgg agatcaaggg gctgacaaca agcattaccg cgttaaacgc
    22561 actagaaacg tcactccgcc aggatgcgtc ttttcatctc aatcagcggg gagccacgca
    22621 gcaggatgcg cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga
    22681 acatgttctt tgactggtgg ttcgccacat caccccgcct ccgccagttt tgctgggcat
    22741 tctggttgct gatgttagtt acgctcattt ttctatcatc gacacaccat gaagagcgcg
    22801 acgcattaat tcgactacgg gcaagtcatc accagcagtg ggccgcactg tatcgcctgg
    22861 tagacaccac tcccttcagc gaggaaaaaa cgctgccctt ttcgccactg gattttcagt
    22921 tatccggcgc gcaactggtt tcctggcatc catccgcgca gggaggcgag ttggcgttga
    22981 aaacgctgtg ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg
    23041 tgagccgttt ttcgttaagc gtggaaggtg atgatctttt gttcacgcta caactggaga
    23101 tgccgcatga gggttaaacg ctggttgttg gcaggtattg cattgtgcct tttaaccggt
    23161 atgcgtgacc cttttaaatc gccggaagat ctatgccgga ttagcgaact tagccagtgg
    23221 cgctatcagg ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa
    23281 aagaaatggc gacgggtgca gcaaaacgat gtgctggaaa acggctggac aattttacag
    23341 ctgacgccag acgtactaac gctgggtacc gggacaaact gcgaaccgcc acaatggttg
    23401 tggcaacggc aaggagatac aaatgaagca atggatagcc gcactactgt tgatgctgat
    23461 acccggcgta caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt
    23521 agctcaggtg ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg tgtcgccaga
    23581 cgtcagcggt acggtgtcgt tacatctaac agatgttccc tggaagcagg cactacaaac
    23641 tgtagtgaaa agcgccggac tgataacgcg gcaggaaggc aacattctct cagtgcattc
    23701 cattgcctgg cagaatgaca atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc
    23761 aaatctgccg ctggaaaatc gcagtataac cctgcaatac gccgacgcgg gagaactggc
    23821 gaaagcgggg gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa
    23881 tcgccttttg ctacgagata acaaaacggc gttaagcgcg cttgaacagt gggtagcgca
    23941 aatggatctg ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa
    24001 aagtttgcgt gagttaggcg tgaaatggac gctggccgat gcgcaacacg ctggtggcgt
    24061 tgggcaagtc accacgcttg gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg
    24121 ttttaacatt gggcgcatca acggacgctt gctggatctt gagctttccg cgctcgaaca
    24181 aaaacagcag ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc
    24241 cagcattaaa caggagagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac
    24301 gtcggtggaa tttaaagagg ccgtcctggg gatggaggtc acgcccacgg tgttacaaaa
    24361 aggtcgcatc cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca
    24421 ggccgatggc gaagtgctgg cgattgataa gcaggagatc gaaacgcagg tcgaggtaaa
    24481 aagcggagaa acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga
    24541 tagcgtaccg ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa
    24601 agaagatgaa cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta
    24661 aacagccgta aaagcggtaa tgtttttatg ctgaacgtgt ttcatctatt tgacgcgcgc
    24721 aggtatttag catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc
    24781 catgatgatg atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc
    24841 ggtgatttat cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca
    24901 gagggatggg tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc
    24961 tgactctcgc aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag
    25021 cgggtttatc attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata
    25081 tctttctggt tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac
    25141 agctcaatat ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg
    25201 tgggctgggt tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca
    25261 tcaatgagtt gaccgagaaa cagggtattg tgctggctac tggcggcggc tctgtgaaat
    25321 cccgtgaaac gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca
    25381 tcgaaaagca acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa
    25441 caccgccgcg tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga
    25501 ttgccgacgt gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta
    25561 ttcacatgct ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa
    25621 ttaaggtgga tgtcgcgtta tggagaggat tgtcgttact ctcggggaac gtagttaccc
    25681 aattaccatc gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg
    25741 cgagcaggtc atgttggtca ccagcgaaac cctggctcct ctgtatctcg ataaggtccg
    25801 cggcgtactt gaacaggcgg gtgttaacgt cgatagcgtt atcctccctg acggcgagca
    25861 gtataaaagc ctagctgtac tcgataccgt ctttacggcg ttgttacaaa agccgcatgg
    25921 tcgcgatact acgctggtgg cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc
    25981 ggcggcgagt tatcagcgcg gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca
    26041 ggtcgattcc tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat
    26101 tggcgcgttc taccagcctg cttcagtggt ggtggatctc gactgtctga aaacgcttcc
    26161 cccgcgtgag ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg
    26221 tgcgtttttt aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc
    26281 aatggcgtac tgtattcgcc gttgttgtga actgaaggca gaagttgtcg ccgccgacga
    26341 gcgcgaaacc gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga
    26401 agctgaaatg gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat
    26461 ggcggcgcgg acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat
    26521 aaccctgctc acgcgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc
    26581 gtatttaccg catatgctgc gtgacaagaa agtccttgcg ggagagatgc gcttaattct
    26641 tccgttggca attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa
    26701 cgccattgcc gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg
    26761 tctattagct tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa
    26821 tggatgaatt caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc
    26881 gttctcgtca atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt
    26941 ttgatgatat tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagagc
    27001 gcaatgagga accggaaatc gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg
    27061 aagagcgcgt agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc
    27121 gtcagtatat gatgatgggc gtcggcattc tggttctact gctgttgatc atcggtatcg
    27181 gttctgcgct aaaagccccc tcgaccactt ccagcgatca aaccgcgtct ggcgagaaga
    27241 gtattgatct tgctggcaat gcgaccgatc aggcgaatgg cgtgcagcca gcgccgggaa
    27301 ccacgtctgc ggaaaatact cagcaggatg tttcgctgcc accgatctct tctacgccga
    27361 ctcaagggca aaccccggtg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc
    27421 tgaacaatgc gctgacccag ccacaaaatc agcaacagct gaacaatgtg gcggtcaatt
    27481 ccacgttgcc gactgaaccc gcaacggttg cgcctgttcg caatggcaat gcatcgcgtg
    27541 actcggcgaa aacgcaaacc gctgaacgtc cgtccactac gcgcccagtt cgtcagcagg
    27601 cggtgattga accgaaaaaa ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac
    27661 agacgccgaa gcgtactgaa ccagctgccc ctgtggcgag cacgaaggca ccggctgcga
    27721 cttctacgcc agcaccaaaa gagacggcga ctacggctcc agtacagacg gcatccccgg
    27781 cgcaaaccac ggcaacacca gccgctggag ggaagaccgc aggtaatgtt ggttcgttga
    27841 aatcggcacc gtccagccat tacactctgc agctgagcag ttcctctaac tacgacaacc
    27901 tgaacggttg ggcgaagaaa gagaatctga aaaactacgt tgtctatgaa acgacgcgta
    27961 atggtcagcc gtggtatgtc ctggtttctg gcgtgtacgc ttcgaaagaa gaggcgaaaa
    28021 aagcggtatc tacattgcca gcagatgttc aggccaaaaa cccgtgggcg aaaccgctgc
    28081 gtcaggtaca ggccgatctg aagtaatcaa ggttatctcc cgcaatggtt tatcgttgcg
    28141 ggagctgcct gaagcgctgg atgctgtcgg agctttctcc acagccggag aaggtgtaat
    28201 tagttaatcg gcatgaagaa aaatcgcgct tttttgaagt gggcaggggg caagtatccc
    28261 ctgcttgatg atattaaacg gcatttgccc aagggcgaat gtctggttga gccttttgta
    28321 ggtgccgggt cggtgtttct caacaccgac ttttctcgtt atatccttgc cgatatcaat
    28381 agcgacctga tcagtctcta taacattgtg aagatgcgta ctgatgagta cgtacaggcc
    28441 gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg tttactatca gttccgcgaa
    28501 gagttcaaca aaagccagga tccgttccgt cgggcggtac tgtttttata tttgaaccgc
    28561 tacggttaca acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt gccgtttggc
    28621 cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag
    28681 aatgcctttt tctattgtga gtcttacgcc gatagcatgg agcgcgcaga tgatgcatcc
    28741 gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat
    28801 cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg
    28861 gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg
    28921 tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc
    28981 acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg
    29041 aaaaaataat tctcaaggag aagcggatga aacagtattt gattgccccc tcaattctgt
    29101 cggctgattt tgcccgcctg ggtgaagata ccgcaaaagc cctggcagct ggcgctgatg
    29161 tcgtgcattt tgacgtcatg gataaccact atgttcccaa tctgacgatt gggccaatgg
    29221 tgctgaaatc cttgcgtaac tacggcatta ccgcccctat cgacgtacat ctgatggtga
    29281 aacccgtcga tcgcattgtg cctgatttcg ctgccgctgg tgccagcatc attacctttc
    29341 atccagaagc ctccgagcat gttgaccgca cgctgcaact gattaaagaa aatggctgta
    29401 aagcgggtct ggtatttaac ccggcgacac ctctgagcta tctggattac gtgatggata
    29461 agctggatgt gatcctgctg atgtccgtca accctggttt cggcggtcag tctttcattc
    29521 ctcaaacact ggataaactg cgcgaagtac gtcgccgtat cgacgagtct ggctttgaca
    29581 ttcgtctgga agtggacggt ggcgtgaagg tgaacaacat tggcgaaatc gctgcggcgg
    29641 gcgcggatat gttcgtcgcc ggttcggcaa tcttcgacca gccagactat aaaaaagtca
    29701 ttgatgaaat gcgcagtgaa ctggcaaagg taagtcatga ataagtttga agatattcgc
    29761 ggcgtcgctt ttgatcttga tggtacgctg gtcgacagtg ctcctggtct tgctgctgcg
    29821 gtagatatgg cgctgtatgc gctggagttg cccgtcgcag gtgaagaacg cgttattacc
    29881 tggattggta acggcgcaga tgttctgatg gagcgcgcat tgacctgggc gcgtcaggaa
    29941 cgtgcgactc tgcgtaaaac aatggggaaa ccgcccgttg atgacgacat tccggcagaa
    30001 gaacaggtac gtattctgcg taaactgttc gatcgctact atggcgaggt tgccgaagag
    30061 gggacgtttt tgttcccgca cgttgccgat acgttgggcg cgttgcaggc taaaggcctg
    30121 ccgctaggct tggtcaccaa caaaccgacg ccgttcgtcg cgccgctgct cgaagcctta
    30181 gatattgcca aatactttag cgtggttatc ggcggcgatg atgtgcaaaa caaaaaaccg
    30241 catccggacc cgctgttact ggtggctgag cggatgggaa ttgccccaca acagatgctg
    30301 tttgtcggcg actcacggaa tgatattcag gcggcaaaag cggcaggttg cccatcagtt
    30361 ggcttaacct acggatacaa ctacggcgag gctatcgatc tcagccagcc tgatgtaatt
    30421 tatcagtcta taaatgacct tctgcccgca ttagggcttc cgcatagcga aaatcaggaa
    30481 tcgaaaaatg actaagccca tcgtttttag tggcgcacag ccctcaggtg aattgaccat
    30541 tggtaactac atgggtgcgc tgcgtcagtg ggtaaacatg caggatgact accattgcgt
    30601 ttactgtatc gttgaccaac acgcgatcac cgtgcgccag gatgcacaga agctgcgtaa
    30661 agcgacgttg gatacgctgg ccttgtatct ggcttgtggt atcgatcctg agaaaagcac
    30721 catttttgtt cagtcccacg taccagaaca tgcgcagtta ggctgggcac tgaactgcta
    30781 tacctacttc ggcgaactga gtcgcatgac gcagtttaaa gataaatctg cgcgttatgc
    30841 cgagaacatc aacgctggtc tgtttggcta tccggtgctg atggctgcgg acatcctgct
    30901 gtatcaaact aatctggtac cggtgggtga agaccagaaa cagcacctgg aactgagtcg
    30961 cgatatcgcc cagcgtttca acgcgctgta tggcgagatc tttaaggtgc cggagccgtt
    31021 tattccgaaa tctggcgcgc gcgtaatgtc gctgctggag ccgaccaaga agatgtccaa
    31081 gtctgacgat aaccgcaata acgttatcgg cctgctggaa gatccgaaat cggtagtgaa
    31141 gaaaatcaaa cgcgcggtca ctgactccga cgagccgccg gtagttcgct acgatgtgca
    31201 gaacaaagcg ggcgtttcca acctgctgga tatcctttct gcggtaacgg gccagagcat
    31261 cccagaactg gaaaaacagt tcgaaggcaa gatgtatggt catctgaaag gtgaagtggc
    31321 tgatgccgtt tccggtatgc tgactgaatt gcaggaacgc tatcaccgtt tccgcaacga
    31381 tgaagccttc ctgcaacagg tgatgaaaga cggcgcagaa aaagccagtg tacacgcttc
    31441 ccgtacgctg aaagcggtgt acgaagcgat tggttttgtg gcgaagccgt aatatcaaaa
    31501 cgctcggacg gtcccctcgc ccctttgggg agagggttag ggtgagggga gcaggcaagc
    31561 gccagtggga attacccttt gctcgcattg tcgaataaat cacttcgtca tcactctgaa
    31621 accggggaaa cccggttttt ttatcctctt ttcgcaacaa cccgaaaaaa tgtgaagcgc
    31681 ctcgccgttt ccacatctcg tcgtcgccac ttatcttcat tttaatgaag atataaatat
    31741 tcattttatt gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgtcatc
    31801 acgacactgg cccgttattt gttgggtgaa aaatgcggta atcgactgaa aaccatagat
    31861 gagctggcaa ctgaatgccg ttcatcggtt ggcctgacgc aggccgcgtt gaaaacgctg
    31921 gaatcaagcg gagcgatacg gattgaacgc cgtgggcgca atggcagtta tcttgtcgag
    31981 atggataaca aagcattgct gagtcatgtg gatatcaaca acgtggtatg tgcgatgcct
    32041 ttgccctata cccgcttgta cgaaggttta gcgagcggat tgaaagccca gtttgatggc
    32101 attccttttt actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac
    32161 ggtgtctatg acatggcagt agtttcccgc ctggcggcgg aaagttatct cagccaaaac
    32221 aatttgtgca tcgccctcga gcttggcccg catacgtatg ttggtgaaca tcaactgata
    32281 tgccgcaaag gtgagtctgg aaatgttaag cgagtaggtc tcgatagtcg ttcggcggat
    32341 cagaaaatca tgaccgatgt cttttttggc gatagtgatg tggaacgagt cgatctctct
    32401 tatcacgaga gtttacaacg cattgttaaa ggcgatgtcg atgcggtgat ctggaacgtg
    32461 gtggcggaaa acgaactgac catgctggga ttagaggcga cgccgctaac agacgatccg
    32521 cgatttttac aggccaccga agccgtggtc ctgacgcgag tcgatgatta cccaatgcaa
    32581 caattgctgc gtgctgttgt agataaacac gccctgcttg cccatcaaca acgggtggtg
    32641 agtggggaac aggaacccag ttattaaacg aaaggcatct gatatggaaa ccagactcaa
    32701 cctgctttgc gaggcaggcg ttattgataa ggacgtctgc aaaggcatga tgcaggtcgt
    32761 caacgtactg gaaaaagagt gccatctgcc ggtgcgcagt gagcaaggaa cgatggcgat
    32821 gacacatatg gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga
    32881 taacgagttg ctggcggaac tggcgcaatc cagccactgg caagccgttg tgcaattgca
    32941 tcaggagttg ttgaaggaat tcgctctgga agttaacccg tgtgaagaag gctattttct
    33001 ggccaatctt tatggattat ggatggctgc taacgaagag gtttgaagct cccgacaaat
    33061 ccacgtcgca caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga
    33121 ggcaacaaga tgtttgtaga agcattgaaa cgccagaacc cggcgctgat ttccgccgca
    33181 ctaagtttgt ggcagcaggg caaaatcgcc ccggacagct gggtgatcga cgtggatcag
    33241 atactggaaa acggtaagcg gctcattgag acggcgcggc tttacggcat tgaactgtat
    33301 ctgatgacca agcaatttgg tcgtaatccg tggctggcgg aaaaattgct ggcattaggc
    33361 tacagcggca ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgctggtttg
    33421 ccagtggcgc atcaggggca tctggtacaa atcccttgtc atcaggttgc tgacgccgtt
    33481 gaacagggca ccgacatcat caccgtgttt actctcgaca aagcgcggga agtttctgcg
    33541 gcagcggtta aggccggacg ggtccagcct gtgctgctca aagtttatag cgacgatgat
    33601 tttctttatc cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtgattgct
    33661 gaaattaaga aactgccggg gctgcattta gccggactaa cccatttccc ttgcctgctt
    33721 tgggatgagg ctgctgggaa agttttgcca acaccgaatc ttcacacgct ggtacaagca
    33781 cgggatcaac tggcgaaatc tggtattgcg attgagcaac tgaacgtacc ttcagcgacc
    33841 agctgcactt cgctgccatt gctggcggaa tacggtgcga ctcatgccga acccggtcat
    33901 gcgctgaccg gtactattcc ggcaaaccag cagggcgatc agcctgaacg tatcgcgatg
    33961 ctctggttaa gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt
    34021 tactaccgtc gtggtcatgc gcaacatgcg ctggtgttta cgccagaaaa tcaaaagatt
    34081 actgaaacca atctcaaaac tgtggatgac agcagtatcg actacaccct gccgctggca
    34141 ggcgagtatc cggtgagcag tgcagtggtg ctctgttttc gcacgcagat ttttgtcacc
    34201 cgtagcgatg tggtgctggt gtccggtatt cagcgcggcg aaccagaaat agttggtcgt
    34261 tatgacagcc tcggcaaccc tctggaggca taaatggcgc gatttgtggt gttagtgatt
    34321 gatagctttg gcgtaggggc aatgaaagat gtcacgctgg tgcgcccgca agatgccggg
    34381 gcgaatacct gtggtcacat cctgagccag ttgccgcatt tgcagctacc aacgctggag
    34441 aagctggggc taatcaacac attgggttat gcgccaggcg atatgcagcc gtcagattcc
    34501 gcaacctggg gcgtggcaga gctgcaacat gaaggtggcg atacctttat ggggcatcag
    34561 gaaattttag gcacgcgccc gttaccgccg ctgcggatgc cttttcgcga tgtgattgac
    34621 cgtgttgagc aggcattagt ttccgctggc tggcaggtgg agcgccgtgg cgatgatctg
    34681 caatttctgt cggtcaatca ggcggttgcg attggcgata atctcgaggc ggatttaggc
    34741 caagtctata acattaccgc caatctctct gtgatctctt ttgacgacgc aatcaaaatt
    34801 ggtcgtatcg tgcgtgacca ggtacaggtc ggtcgggtca ttacatttgg cggcctgtta
    34861 accaacagtc agcgtattct cgatgccgca gaaagcaaag aagggcgctt tattggtatt
    34921 aatgcgccgc gttctggcgc ttatgacaac ggcttccagg tcgtgcatat gggctacggt
    34981 gttgatgaaa aagtgcaggt gccgcaaaaa ctgtatgaag cgggcgtgcc aaccgtgctg
    35041 gtgggtaagg tggcagatat cgtcagcaat ccttatggtg tgagctggca aaacctggtg
    35101 gatagccagc ggattatgga tatcaccctc gacgaattta acacccatcc gacggcgttt
    35161 atttgcatca atatccagga aaccgacctc gctggtcatg cagaagacgt cgcacgttat
    35221 gccgaacgtt tgcaggtcgt tgaccgtaac cttgcccggc ttgttgaggc gatgcagcca
    35281 gatgattgcc tggtcgtgat ggcggatcac ggtaacgatc cgaccattgg tcacagccac
    35341 catacccgtg aagtggtgcc agtgctggtt tatcagcaag ggctggtcgc cacgcagctc
    35401 ggtgtgcgca ccacgctttc tgatgtggga gctaccgtgt gtgaattttt ccgtgcgcca
    35461 ccgccacaaa atggtcgctc ttttctttcc tccctccggt ttgcaggaga caccctatga
    35521 gttttgatcc gacgggttac accctggccc atgagcatct gcatattgat ctctccggct
    35581 ttaaaaacaa cgtggactgc cgccttgatc agtatgcgtt catttgccag gagatgaacg
    35641 acctgatgac ccggggtgta cgtaatgtga ttgagatgac caaccgttac atggggcgca
    35701 atgcgcaatt tatgctcgat gtaatgcgcg aaaccgggat caacgtggtg gcctgtactg
    35761 gttattacca ggatgcgttt ttcccggaac atgtggtgac ccgcagcgtg caggaactgg
    35821 cgcaggagat ggtcgatgaa attgaacagg gtatcgatgg cacggagctg aaagccggga
    35881 tcatcgcgga gatcggctcc agcgaaggaa agattacgcc gctggaagag aaggtattta
    35941 ttgccgctgc gctggcgcat aaccagaccg gacgcccgat ctccacgcat acgtcgttca
    36001 gcaccatggg gctggaacaa ctggctctgt tgcaagccca cggagttgat ctttcgcgcg
    36061 tcaccgttgg tcactgcgat ctgaaagaca acctcgacaa cattttgaag atgatcgatc
    36121 tcggcgcgta cgtgcagttc gacaccattg gtaagaacag ttactacccg gacgaaaagc
    36181 gcattgcgat gctccacgcg ctacgtgacc gtgggttgct gaaccgcgtc atgctgtcga
    36241 tggatattac gcgccgctcc catttaaaag ccaatggtgg ttatggctat gactatttat
    36301 tgaccacctt tattccgcaa ttgcgccagt caggattcag tcaggccgat gtggatgtga
    36361 tgttacgtga aaatccctct caatttttcc aataaggaca gactcatgaa aaagattggc
    36421 gttgcaggct tacagcgtga gcagattaaa aaaaccattg aagcgacggc tcctggctgt
    36481 tttgaagttt tcattcacaa cgacatggaa gcggcaatga aggtgaaatc cgggcaacta
    36541 gattattaca ttggcgcgtg taataccggt gcgggcgcgg cattgtcgat tgccatcgcg
    36601 gtgattggct ataacaaaag ttgcaccatt gccaaaccag gcattaaagc gaaagacgag
    36661 catatcgcca aaatgatcgc tgaagggaaa gtggcgtttg gcctttccgt tgagcacgtc
    36721 gaacacgcga ttccgatgtt gattaaccat ctgaaataaa aggcacgact atggatctgt
    36781 atattcagat tatcgtggtg gcgtgcctga cgggtatgac atcgcttctg gcgcatcgct
    36841 cggcggctgt ttttcatgac ggcatccacc cgatcctgcc gcaactgatt gaaggctata
    36901 tgaaccgtcg cgaggcgggg agtatcgctt ttggtctgag cattggtttt gtggcctcgg
    36961 tggggatctc ttttaccctg aaaaccgggc tgctcaacgc atggttactc tttcttccta
    37021 ccgatatcct cggcgtactg gcgataaaca gcctgatggc gtttggtctt ggcgctatct
    37081 ggggcgtgtt gatccttact tgcctgttgc cagttaacca gctgctgacc gcgctgccgg
    37141 tggatgtatt aggtagcctc ggggaattaa gctcgccggt ggtttctgct tttgcactgt
    37201 tcccgctggt ggcgattttc taccagtttg gctggaagca aagtctggtc gccgccgtgg
    37261 ttgttctgat gacccgtgtg gtagtcgtgc gctatttccc acatcttaac cctgaatcca
    37321 tcgaaatctt tattggcatg gtgatgctgc tggggatcgc gataactcac gacctgcgtc
    37381 atcgtgatga aaatgacatc gatgccagcg ggctttcggt gtttgaagag cgcacgtcgc
    37441 ggattatcaa aaacttacct tatatcgcca tcgtgggagc attgattgcc gccgttgcca
    37501 gtatgaagat ttttgctggt agcgaagttt cgatcttcac actggagaaa gcgtattccg
    37561 caggcgtgac gccggaacaa tcgcaaacgc tgattaatca ggcggctctg gcagaattta
    37621 tgcgcggact ggggtttgtg ccgttgattg ccaccaccgc cttagcaacg ggtgtgtatg
    37681 cagttgcggg ctttaccttt gtttatgcgg tgggctatct ctcgccgaat ccgatggttg
    37741 cagcggtatt aggcgcagtg gttatttcgg cggaagtctt gctgcttcgt tcgatcggca
    37801 aatggctggg acgctacccg tcggtgcgta atgcgtcgga taacatccgt aacgccatga
    37861 atatgctgat ggaagtggcg ctgctggtcg gttcgatttt cgcagcaatt aagatggcgg
    37921 gttataccgg attctctatc gcggttgcca tttacttcct caacgaatcc ctgggccgtc
    37981 cggtacagaa aatggcggca ccggtcgtgg cagtaatgat caccggtatt ctgctgaatg
    38041 ttctttactg gcttggcctg ttcgttccgg cttaaggaag gcacctatga agacgtttcc
    38101 tttgcaaagc ctgactcttg ctgaagcgca gcaaaagcag tttgcgctgg tggacactat
    38161 ttgccgccat tttccaggca gcgagtttct tgctggcggt gatttagggt taactccaag
    38221 tctgaatcaa ccgcgtatta cccaacgggt ggagcaggtg ctggctgatg catttcacgc
    38281 acaggccgcg gcgctggtgc agggcgcggg gactggcgcg attcgcgccg ggctggcggc
    38341 tttgctcaaa ccggggcagc gtcttctggt gcatgacgcg cctgtttacc cgacgacacg
    38401 ggttattatt gagcagatgg ggctgacgct tattactgct gatttcaatg acctgtcggc
    38461 actgaagcag gtcgtcgacg agcaacaacc tgatgccgcg ctggtgcagc atacgcgcca
    38521 gcagccgcag gacagctatg tgctggcaga tgtgctggca acgttgcgcg cggcaggtgt
    38581 tccagcgtta accgatgata actatgcggt gatgaaggta gcgcgaatcg gctgtgaatg
    38641 cggcgcgaat gtctcgacat tctcctgctt caagctgttt gggccggggg gcgttggtgc
    38701 agtggttggc gatgccgatg ttattagtcg gattcgcgcc acgctttact ccggcggtag
    38761 ccagatccag ggctcacagg cgctggaagt cttgcgtggt ctggtgttcg cgccagtgat
    38821 gcatgcggtg caggcgggag tatccgaacg gttgctggct ttgcttaacg gcggcgcggt
    38881 ggcggaagtg aaaagcgccg tcattgccaa tgcgcagtcg aaggtgttga ttgtcgagtt
    38941 tcatcagctg attgccgcca gggtgctgga agaagcgcaa aagcgtggcg cgttgcctta
    39001 cccggtcggt gcagagtcga aatatgaaat cccgccgctc ttctatcgac tttcaggaac
    39061 gtttcgccag gcgaatccac aactcgaaca ttgtgcgatt cgcattaacc cgaatcgcag
    39121 cggtgaagag acggtgctgc ggattttgcg tgagagtatt gcgagtattt aatgctggat
    39181 agcaccgttc ggcaatcaag ctaaattccg cactgaacgg tcccctcgcc cctttgggga
    39241 gagggttagg gtgaggggaa cgtgcgggat gtaaaattca gtgcgccagc atcgccggat
    39301 gcgatgctgg cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg
    39361 tgatgcgatt ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa
    39421 aggcgatttt gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct
    39481 gcgggcgggt ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa
    39541 aacgctcctg aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag
    39601 gataacgcga cagcgggatc catgaactat caataaacag cggctcttta tcgagataca
    39661 tcacccggca gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg
    39721 gcgcggcgct gacccgttcc tgttcgatca ctttctcttt cgtcgacttg ccttgtgaga
    39781 cgccaaaatc ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta
    39841 caaaggtgcc ttttccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatgg
    39901 ctttgcgaat ggtaatgcgg ctgacgttat attgtgtaca aagctcgttt tcggtaggga
    39961 tctgttgccc ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg
    40021 tagcgtagag gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa
    40081 agaatgaatt actcggatta tagggtcgtt ttccggcaag taacagcacg ttggcgtgaa
    40141 gatgctcacg ccaacgttat acctaccagg caccgtggta ctgaatggtt ttcgccgcgc
    40201 acgccgttcc ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga
    40261 atccggcaat gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag
    40321 cctgacgcca gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa
    40381 tcactgttcc tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt
    40441 cttgcggtgc ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc
    40501 tgtcccactt gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga
    40561 atgcgtcttc cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg
    40621 cgtaatcctc ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa
    40681 cgcgatcatt gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga
    40741 catggctgat atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat
    40801 cgtcacccac ccaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca
    40861 cattgaccgc attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt
    40921 tatcgccgat tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg
    40981 ttccagcgcc tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac
    41041 cgtacagtaa ccctcatagc cccgatcaat aatatcgcgc atcagttccc gcagcggcat
    41101 tttgccttct ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag
    41161 atgacgtaat ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata
    41221 cggcgcgcaa atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg
    41281 aagcacatca ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat
    41341 cagatccatg ccgatgtttt ccgcgtactc acacagttcg cttaagttct ctgccagccg
    41401 cccccagata acgttaggcg gcgtgagata gcccgcgtgg gccgcagaaa tcagcgtata
    41461 acccgcgttc atctcttttg ccatatctat cgccagcttg atcatgtcca ggctttcacg
    41521 acgcatatgt tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtgta
    41581 accgataatc ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc
    41641 ttttaagtcc ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc
    41701 cagctcgctt gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa
    41761 catacctgtt ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga
    41821 gggattaata ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt
    41881 aaatagacag gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc
    41941 acgggtgcag cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct
    42001 gtttaacaaa gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga
    42061 acaggaacgg aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct
    42121 caatcacgca gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac
    42181 ccagcggacg cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt
    42241 caccaagctg gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca
    42301 actgcttgag atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca
    42361 tctccagcac cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat
    42421 aatcaatgct aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg
    42481 cagtgagtgc gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac
    42541 cgtagtcaga aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc
    42601 agaactccca gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt
    42661 tcgccgtttg cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt
    42721 cgcgcttcac catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt
    42781 tttccacttc ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat
    42841 ccaacatttt attttcttca gataacatta cgacaaagcg ttgagctggc ggctacgctt
    42901 cgcccagaag gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc
    42961 gggaatcggt gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag
    43021 ggtggtcatc agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg
    43081 acaccagatg atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata
    43141 acccagcagg ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag
    43201 gatgatggag acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa
    43261 caggttgtct ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag
    43321 cgaaccaaga atgacgatca tcgccgtgat ggcaacaaag agcccagcgg tgctgccgag
    43381 tgcggggatc taggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa
    43441 gggcatcagg ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca
    43501 ggaaccaatc agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata
    43561 acagatagag gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc
    43621 cataaagctg cccgttgcgc caatcgcagt ggtggtaagg gcggcaaaat tctccgcttt
    43681 aaaccagaag atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat
    43741 tagcgtctga aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat
    43801 aattaatccg gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag
    43861 attgctgaca atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct
    43921 ggcccagccg gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc
    43981 tgcgccattt tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac
    44041 aattaacccg ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc
    44101 cacttcaccc acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag
    44161 aacggcccaa aatccgagct tgcgttggag ttcttggctt cccatagcga tacctttttc
    44221 ttgtccaata cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat
    44281 gatgaatata acaacatatt atgttatgta atgtggagga agatcacaaa atttcgcaca
    44341 ggatcgcgct gtggctaatg gatgtagtta taaaattgaa tttaaagtga aaatattttt
    44401 acgggcgggg gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc
    44461 gggcaattaa accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca
    44521 accgccaatg attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt
    44581 cttatcacgg ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac
    44641 cagtgcaact ttaataaatt tgttcattta cattccctat gtattatata ttaattaata
    44701 ttcattgatt atatgaatat aagcagactg cttgttaaga acgatacagg aatatttaat
    44761 tggcgtcagt gagcgaatga cttaattttg taagtcgctg taacggtgaa tataacccat
    44821 tagcagataa tatttattaa ggggttatgt tttatttgct gaatatataa aaagagtcat
    44881 taagaaggcc agaattctct ggcccgggtt aaattaatga ttggagaacc agttcagttt
    44941 atcgcgcagg ccaacaaccc gaccaataat aattagcgat ggactgttca tttgctgagc
    45001 aagttcaccg agttgcgtga gcgtaccgtc aatcacgcgc tgcgtgactg ccgttccgtt
    45061 ttcgacaatt gccaccggca tttcgccagg cataccgtgt tcaatcagct tttgctgaat
    45121 agtcgcggcc tgattcaacc ccatatagaa caccagcgtc tgtttttctg ccgccaggtt
    45181 ttcccagtcc agctcgccac cggtttttaa gtgtccggta attaagcgta cgctctgggc
    45241 ataatcgcga tgcgtgagtg gaatacccga ataggcagag caaccagaag ctgcggtaat
    45301 acccggaacc accgagaacg gaatacccgc gttgcacagt gtttccagct cttcgccacc
    45361 acggccaaaa ataaacggat cgccaccttt cagccgcacc acgcgtttgc ctttttgcgc
    45421 ttcccgcagc aggatctggt taatctcttc ctgcggtaca cagtggtatc ccgcgcgttt
    45481 gccgacgaaa acgcgatcag catcgcggcg taccagattc ataatatcgt cagaaaccag
    45541 acggtcgtag accaccacat ctgcctgctg aatttgttgc agtcctttca gcgtcagcag
    45601 cccggcatcg cccggacctg caccaaccag caccacttca ccgcgatggt cgagcggttc
    45661 gttgattaac tgttcggtcg tttcagtaat ggctttctga tcgttgtttg ccagcgactg
    45721 cgccaggcgg tcgttaacga acaatttctc ccagaaacgg cgacgctcac tcatcgtggc
    45781 gaactgttgt ttcactcgcc cgcgtaattg cccggcatat ttcgccacct ggcccagatg
    45841 caacggcagc agtgattcga gtttttcgcg caacaggcgc gccagaaccg gagaggtgcc
    45901 gccagaggag accgctacca ttagcggtga gcggtcaata atcgacggca taataaagct
    45961 ggcggctttc ggcgcatcaa ctacgttaca gaagatgcga cgagcttcag cggcttcgct
    46021 gacgcgctgg ttaagcgtgt catcatccgt cgctgcaatc gccagccagc aggtgtcgag
    46081 aaggctttca tcaaatggcc cttcgacgag ggttaacatg cctgcatctg cccatgcggt
    46141 gaactgtgga ataaacgcta atgcattcac cgttaagcga gcgcctgcgt ctaacagcaa
    46201 ccttgctttg cgttccgcga catcaccacc gccgacaatc agacagtcgc gatcgcgtaa
    46261 ttggcaaaat ataggcaaat gatccacgta aaaacccctt agtaattaac cggcagctgt
    46321 ttcagtttga ttaaatttgt ccgcaaccgg acgattcgct ttcggcgtag cataccaata
    46381 acccaatccc atgaatacgg cacctgataa agtattaccc agcgtcaccc acagcaggtt
    46441 atgaccaata cccgccagcg tgtaggcttc gctgtggttg ccgaaccagg agagcgcgaa
    46501 cagcgtcatg ttagcgatag agtgctcgta gccggacgcg ataaatgcca gcagacacca
    46561 ccagatagcg ataaatttcg ccgccccttc agtgcgcagc gccatccaga ttgctaggca
    46621 aaccagccag ttacacaatg cacctttgaa gaacagtacc attgccggtg cagtggtttt
    46681 agccagcgcg acggagtgaa cgatgctggt atctaccggc agcaggctac cgccgcccca
    46741 gctatagagc atggcaacga agacggaacc gaccaggtta cccagccagg tttgcggcag
    46801 gattgcccac atttgcccgt ggctgatgct gcccgctttt accccaaagg tgaggaacat
    46861 ggtgtgtccg gtgaacagtt cagaaccggc gataatcacc agcgttaagg cgataccaaa
    46921 ggtcgcgccc atcaccagcg gacgtacgga cgggtcgagc aaattaccga gcgtgaaaat
    46981 caggatgatc ccaagaccca catatgcgcc cgccatggcg gagctgaccc aaaagccgag
    47041 cgggttattt gccgacaggc gtgcaatgcg cgcagcatta gccgcacact tattaatagt
    47101 gtctgtaaac atttgattat cctttaaaaa ataaaaacaa aaaataaaac aaaaagttta
    47161 aaaaatgtcc tgcaatatac cgaaggtcat tacaggacca tgcaaaaagg ggaggcattg
    47221 cgcctcccgt taaaacatta accgcgcagc tgcaccacgc cgtctttcac tcgtgcttcg
    47281 taatgtttga cggaaaactg ttcgtcttcc atgcacaagc catcacttaa gcgaaaacgc
    47341 tgttttttca gcgggctggc gacccacagc tcgccctggt gttccgcaat cagcccgcgt
    47401 gatagcacgc tggactcgaa gaacggatca atgttgctga tcgcaaacac ctggtcgctg
    47461 tgatacgggc ggaaaatcgc tacctgcttg tcacctaaca gtgcgcagac gccggtttca
    47521 ggcaggatgt catcgatttt gcagatgtct ttccactggc tcatgcgttg tcctccacca
    47581 gagttaccgg gatacgttca tacggcgttg ccggacggtg ctgttcgcgc tctggcacca
    47641 tctgcacgtt cgggtcacgc ttgtcgctgt tgatgaagtg tttgaagcga gtctgcgcgg
    47701 acggtgtatt gaccgtttca gtccactcac acactaccgc ttcacgcagg cgcgccatct
    47761 cttcttccag atgagcgttc agccccagtt tgtcgtcaat gatcaccgct ttcaggtaat
    47821 cgatgccgcc ttcgaggttt tctaaccacg gcgcggtacg cgtcagtttg tcggcggtac
    47881 ggatgtagaa catcatgaag cggtcgagat atttgatcag cgtttcgcga tcgatatccg
    47941 ctgccagcag atccgcatga cgcggtttca tgccgccgtt accgcaaaca tacaggttcc
    48001 agcctttttc agtggcgata atacccacgt ctttaccctg agcttctgaa cattcacggg
    48061 tacagccgga gacaccgaac ttcattttgt gcggcgtacg gatgcctttg tagcggtttt
    48121 ccagttccac gccgaggccg acgctatcgc caacaccgta gcggcaccag gtgctaccca
    48181 cgcaggtttt cgccatacgc agtgctttcg cataggcatg accggtttcg aagccagctt
    48241 caatcagctg acgccagatt tccggcagat cgtctttctg tgcgccaaac atcgccaggc
    48301 gctgggagcc ggtgattttg gtgtagagat taaattcacg cgcgatacga cccactgcca
    48361 tcagcccttc cggggtgatt tcaccgcccg gagaacgcgg gatcaccgag taggtgccgt
    48421 ctttctggat gttagcgagg aagttgtcgt tagaatcctg cagcggagta tgttccggct
    48481 tcagaatgta ttcgttccag caggaggcca gcagcgaacc gacggttggt ttacaaactt
    48541 cacaaccgta gcctttgccg tgtttcgcca gcagttcttc gaaggtttta atgccttcaa
    48601 cgcggatcaa atggaacagt tcctgacgcg aataagcaaa gtgttcgcac aggttgttgt
    48661 taacttcgat gccctgtttc gccagttccg cgttcagtac ctgagtgacc agcgggatac
    48721 agccaccgca gccagtaccc gctttggttt cagctttcag cgccgcaact gtgtggcagc
    48781 ctttgttgat ggcagcaatc agatcacctt tagtgacgtc gaagcaggag cagatttgcg
    48841 cgctgtccgg cagtttatca acaccgatag acggcttacc gctacccgag tgcgccggca
    48901 gaatcagaga atccgggttt tccggcagct cgatagcgtt cagcaccagt tgcagcaggt
    48961 tgccgtagtc gctggtatcg cccaccagta ccgcaccgag cagggttttg ttgtcttcgc
    49021 tgacaatcag gcgtttgtag atctctttac tttcgtcgag gtaaacatag ctacgtgcgc
    49081 caggcgtgcg accgtgcgca tcaccaatac cacctacgtc tacgcccagc agtttcagct
    49141 tggcgctaag gtcagcacct tcaaaggcgt tttcgctacc cagaatatgg tcaacggcga
    49201 cctgcgccat tttgtagcct ggtgctacca gaccaaatac acggttgttc cagcttgcgc
    49261 attcaccgat ggcgtagata tccggatcgg aagtctggca ggaatcatta atgacaatcc
    49321 ccccacgcgg agcaacgtcc agaccacact gggttgccag cttatcgcgc ggacggatac
    49381 cggtagagaa gacgataaag tcgacttcca gttcgctgcc gtcggcaaaa cgcatggttt
    49441 tacgcgcttc gacaccttcc tgcacaatct caagggtgtt tttgctggtg tgaacgcgca
    49501 cgcccatact ttcgattttg cgacgcagct gctcgccacc catctgatca agctgttccg
    49561 ccatcagcat aggggcaaat tcgataacgt gggtttcaat acctaagttt ttcagcgcgc
    49621 cagcggcttc cagacctaac aggccgccac caacaacggc accgcgtttg ctgcgacggg
    49681 cgcaggattc aatggcgttg aggtcttcaa tggtgcgata gacaaagcag tcctgagtat
    49741 cagaaccttt gattggtggg atccacgggt aggaaccggt tgccatgatc agcttgtcat
    49801 aaaaaacggt acgtccggcg ctggagtgaa tcaccttctc ctgacggttg atggtgatag
    49861 cgcgttcgcc gaccagaact ttgatgccgt gtttctcgta gaagccttcg cgcaccagcg
    49921 acagctcttc ggcggtgtgg tgagagaagt aagacgagag gtgtacgcgg tcataagcga
    49981 tgcgcggttc ttcacagaaa acggtaatat caaagttggc cgcatcagat ttatcaagaa
    50041 gatcttcgat aaagcgatgg ccgaccatac cgttaccgat aattgcgagt ctgactttgc
    50101 tcatttttgc ctcgatttct tttctattac cgcctacctt aacgattcag caaccccgct
    50161 tattgatgta aatcaaattc acctttatat actccttaag gggtatattg ctgatttgta
    50221 taaattttct taagtcacgg aattgtctga catttcatgt ttgtgcaaaa aatggacaaa
    50281 atgaacgggt ttatagaaat agatgcacgt agcgggggaa gagaggaaaa ggccggacga
    50341 cgatagtgcc gtccggcatt agcaattaat gagaggtcag ggtgttatgc tgacggtgac
    50401 ggctgacgaa acctaacagg aagcacatca caaagacgac agcgtacaga ccgtttgctg
    50461 taagcagtgc cgcctgcgga ccgctatgtt caacaatcgg gccggtaacc acaaaggtca
    50521 acatagtacc gatggttccg caggtcagga caaagttaac cagttttggc gacggtactt
    50581 tggtctgctg tgaacccagg gtgatgatgg tggtatagat cgcgctggag aagaagccca
    50641 gagcgagaat tgaccacgcc atatgtactg gtgttccggt gttaaagacg tacatcagaa
    50701 tcgcagccag accagccagt acggtcagaa tgcgttgcaa atcaaagaag cgaagaataa
    50761 agctgaacgc ccacatgccg accatgtatg acatccagaa gttactcacc agcgtgcccg
    50821 cgtcgttcag gctcatgccc aggcctttgg catactcagg cacccaggag ataaaaccta
    50881 actgaccgag gatgtagcac agcgccgcaa cagagagaaa cagtacgccg atcccccact
    50941 tttctttctc taccggagca tccgtttttg gtgcacgttt gcccagtgcc gggaactcac
    51001 agccgaaggt cagaataaaa atagcgacat acaccagccc gatgcaggca taaacccagt
    51061 accactcaat gctgcgcgcc agtagaaacg cggcgatcat tgggaaaatc atcccagcca
    51121 tactgaagaa ggagtcagta aataacaggc gggaaccgcg ctgacgccct tcatacattt
    51181 gtgttatcag gaatgtacca atcgacatag taatgccgct gaccaccccg agaatgaaca
    51241 tcgccgtcga gaacagcgcc aggctgtggc tgaacatcaa accggcaacc gccagcacca
    51301 tcaggagaaa gccaaaacgt aactgcgttt tcaacgggac gatttccatc agccaggcgt
    51361 tgaggaagat agagattaaa atgccggcgt tgaggaaggt gaaggtgtta ctcatactgg
    51421 aaacaggcag attgaaataa tcggcgatat ttcccatcac catcccggtg acaataacca
    51481 acgcaccagt cagtgcgtag gagagaaagc taatccatgt gagcttgatg cgattgctgt
    51541 tagtcatgat tggcctgcgt tcaaaaataa aatggcatag cgggatatgc cgcgagcggg
    51601 cgattttagg tgattttatg atctgtttaa atgttttatt gcaatcggtt gctaaattgc
    51661 attttaagag gtgattttga tcacggaata aaaagtgatc gtcaggttac atatatttca
    51721 gatacgtaaa attaggtaaa gggatggcct tgttcttgaa ggctatttag aatctcttca
    51781 cttgcttttt ttctgctctg tttgttaagg aaatctcatg ttcaaatcga ccctggcggc
    51841 gatggctgct gttttcgctc tttctgctct ttctgctctt tctcccgcag caatggcagc
    51901 gaaaggggac ccgcacgtat tgttgacaac ctcagctggt aacatcgaac tggagctgga
    51961 taaacaaaaa gcgccagtgt ctgtgcaaaa ctttgtcgat tatgtgaaca gcggttttta
    52021 taacaacact acctttcacc gcgtcattcc tggctttatg attcagggcg gcggtttcac
    52081 cgagcagatg cagcagaaaa aaccaaaccc gccaatcaaa aatgaagccg ataacggcct
    52141 gcgcaacacg cgtggcacca tcgcgatggc gcgtaccgct gacaaagaca gcgccaccag
    52201 ccagttcttt atcaacgttg ccgataacgc cttccttgac catggtcagc gtgatttcgg
    52261 ttacgcggta tttggtaaag tggtgaaagg catggacgtt gccgataaga tttcccaggt
    52321 gccgactcat gatgttggtc cgtaccagaa tgtgccgtca aaaccggtag ttatcctttc
    52381 cgctaaagtc ctgccgtaat gatttctcgc gcgggcaatc ttgcccgcgc ttctgctctc
    52441 ccggcgtaac ccggatttgc cgcttatact tgtggcaaat ggacacgttc agggaggcat
    52501 caagtgaaga aactcaccga taagcaaaag tcccgtctct gggagcttca gcgtaatcgt
    52561 aatttccagg ccagtcgccg tcttgaaggc gtcgagatgc ctttagtcac tcttactgcc
    52621 gcagaggctt tagcgcgcct tgaagagctg aggaggcact atgagcgata aattcggcga
    52681 agggcgcgat ccgtatcttt atcctggcct tgatatcatg cgtaaccggc tgaacatccg
    52741 ccagcagcag cggctggaac aggccgctta cgaaatgacg gcgctgcgtg ctgcgaccat
    52801 tgagcttggt ccgctggtgc gcggtttacc gcatttgcgc actatccatc gccagctgta
    52861 ccaggatatt ttcgactggg cagggcaact gcgtgaagtt gatatttatc agggcgatac
    52921 gccgttctgc cactttgctt atatcgaaaa agagggcaat gccctgatgc aggatctgga
    52981 ggaagaaggt tatctggttg gcctggagaa agcgaagttc gtcgagcggc tggcgcatta
    53041 ctattgtgaa atcaacgtgc tgcatccctt ccgggtggga agtggtctgg cacagcggat
    53101 cttcttcgag caactggcga ttcatgccgg atatcaactg agctggcagg gtatcgaaaa
    53161 agaggcctgg aatcaggcaa atcagagtgg ggcaatgggg gatctcaccg cactgcagat
    53221 gatatttagc aaagtggtaa gcgaagccgg ggaatctgag taaaatagcg cggttctttc
    53281 gtaccggagc cgccatgatc ctgcttatag ataactacga ttcttttacc tggaacctct
    53341 accagtactt ttgtgaactg ggggcggatg tgctggttaa gcgcaacgat gcgttgacgc
    53401 tggcggatat cgacgccctt aaaccacaaa aaattgtcat ctcacctggc ccctgtacgc
    53461 cagatgaagc cgggatctcc cttgacgtta ttcgccacta tgccgggcgc ttgccgattc
    53521 ttggcgtctg cctcggtcat caggcaatgg cgcaggcatt tggcggcaaa gttgtgcgcg
    53581 ccgcaaaggt catgcacggc aaaacctcgc cgattacaca tagcggtgag ggcgtatttc
    53641 gggggctggc aaatccactt accgtgacac gctaccattc gctggtggtg gaacctgact
    53701 cattaccagc gtgctttgac gtgacggcct ggagcgaaac ccgagagatt atggggattc
    53761 gccatcgcca gtgggatctg gaaggtgtgc agttccatcc agaaagtatt cttagcgaac
    53821 aaggacatca actgctggct aatttcctgc atctctgatt tccgattgcc attcagtgat
    53881 tttttatgca tattttgtga ttataatttc acatttattt atgcgtaaca gggtgatcat
    53941 gagatggcaa ttgaacaaac agcaattaca cgcgcgactt tcgatgaagt gatcctgccg
    54001 atttatgctc cggcagagtt tattccggta aaaggtcagg gcagccgaat ctgggatcag
    54061 caaggcaagg agtatgtcga tttcgcgggt ggcattgcag ttacggcgtt gggccattgc
    54121 catcctgcgc tggtgaacgc gttaaaaacc cagggcgaaa ctctgtggca tatcagtaac
    54181 gttttcacca atgaaccggc gctgcgtctt gggcgcaaac tgattgaggc gacgtttgcc
    54241 gaacgcgtgg tgtttatgaa ctccggcacg gaagctaacg aaaccgcctt taaactggca
    54301 cgccattacg cctgtgtgcg tcatagcccg ttcaaaacca aaattattgc cttccataac
    54361 gcttttcatg gtcgctcgct gtttaccgtt tcggtgggtg ggcagccaaa atattccgac
    54421 ggctttgggc cgaaaccggc agacatcatc cacgttccct ttaacgatct ccatgcagtg
    54481 aaagcggtga tggatgatca cacctgtgcg gtggtggttg agccgatcca gggcgagggc
    54541 ggtgtgacgg cagcgacgcc agagtttttg cagggcttgc gcgagctgtg cgatcaacat
    54601 caggcattat tggtgtttga tgaagtgcag tgcgggatgg ggcggactgg cgatttgttt
    54661 gcttacaggt actacggtgt gacgccggat attctgactt ctgcgaaagc gttaggcggc
    54721 ggcttcccga ttagcgccat gctgaccacg gcggaaattg cttctgcgtt tcatcctggt
    54781 tctcacggtt ccacctacgg cggtaatcct ctggcctgtg cagtagcggg cgcggcgttt
    54841 gatatcatca atacccctga agtgctggaa ggcattcagg cgaaacgcca gcgttttgtt
    54901 gaccatttgc agaagatcga tcagcagtac gatgtgttta gcgatattcg cggtatgggg
    54961 ctgttaattg gcgcggagct gaaaccacac tacaaaggtc gggcgcgtga tttcctgtat
    55021 gcgggtgcag aggttggcgt aatggtgctg aatgccggac cggatgtgat gcgtttagca
    55081 ccgtcgctgg tggtggaaga tgcggatatc gatgaaggga tgcaacgttt cgcccacgcg
    55141 gtggcgaagg tggttggggc gtaaataaga cgtatcaggc aaaatatgtc ggatgcggcg
    55201 tgaatgcctt atccgatggt ttagcctgta ttacgccttc gaatcccgca acttgcgact
    55261 cagccaaatc ccgtgatgcg gtcgctgacg ccatgccatc gacgaaatgg tgtgcatggt
    55321 gttcagatgg ccaataaccc gctgtaaatg ctgctccagc gtacctgccg cgccttcgtg
    55381 cggctgcatc tccggcgcat ccatgatatt ggcatcgcca gaactacccg gttcgtcata
    55441 ctccagtcgc tgctgacaac gctgaatggc gatttcacaa gactgtaaat actcttgtgc
    55501 cagttcaggt ggcaatgccc ggtgttcccg cgccagcgtg gtcatggcat taatatgctc
    55561 aacaataaac tggctgtgcg ttacccacag tttcatatct gccagataat ggctgttgaa
    55621 tgccggttcc tgcatcgcct gattcaatga gttatacaga gtgttatgcg cctgatttac
    55681 ccgcattctc tgccaggcca gtggcgttgg ttgcgggtcc tcgctcagaa tcaagcgaat
    55741 cgcttcctga taggcttcta aagcatcatg ggcgttttta cgcaataacc cgctctgcca
    55801 ctgcggccac agccagacgg taccaccgaa agcaatcaaa caaccaataa tggtatcgat
    55861 aagacgtgga aggatgtact gctcaccgtt cagccacaac agttgcaggg tatacactgc
    55921 ggtaatagta aaaccgaccg ttgcccagcc gtagtttttg cgcaatatca ggtagctggc
    55981 gagggtggta atcagcatca acgtcagggt gtaactttcg ggaattttaa agtgcagcgc
    56041 tacgcccgca atgattaacc cgaccacggt tcccacggag cgattcacaa tcctcagacg
    56101 ggttgcgcca tagccatttt gtgtcaccag caataccgtc atcaggatcc agtacgactt
    56161 cggcagatgc agcacggtgc ccatcaggct ggcaacgctt aacatcacac tgagtcgtcc
    56221 ggcattgcgt agcgccggag actttagtga cagataactt ttcagtgccg gaagcaacgg
    56281 cattcgccgc tgtttatcgg ccagtaagtc acgggcatag agcggttttt gggtgcgcag
    56341 cacgcgggcg atgcggctga aatgccagta gcagaattgc ccaaccggat tatccggatg
    56401 ctggcgggcg attttttcca gtgcgccaat ttgcttttcc atcgtaaaac gcgttggcag
    56461 gcgatggtaa agaatgtcat cagccagcac gcgcaggcga gcggcgacgg tttgcgcatt
    56521 ccagcggata acttcttccg catggctacg ctcgaccagt ttttgcacct cttccggctg
    56581 atgcaaactg accgaaatat gttcctgcaa atccagcgcc tcctggaaaa tacgcagcat
    56641 ccgcttgtaa tcggtattgt tttgcgcgga aagcatatgc atttgctgat agcactgagt
    56701 aattaaatcg accgcttttt gctggcgcac cagcagcggt ggcagcgctt tttcaggatc
    56761 ggtgtgctgg gtaagcaggc tgtatttggc ttcacaataa tctgccagtt cacggtacag
    56821 cagacttagt gactcgcgca gcggttgttc gcgccagatc cagaaccaaa accagttaaa
    56881 caatccgtac cagagagtgc ccaacgcata gatgagcaat ggttcccaga ccggcatgta
    56941 tcccgccaga ctgagggtaa aaatggcggc gagcagcgat gcaggaagca attttgcgtg
    57001 caatggcccc agctcagcag tgacgccaag taccagcgtt aatccggtca gcaaaaaggg
    57061 caggggaaca tcttttacca gtagcagctg tgtcagtaag ctacaggtgg caaacagcga
    57121 cgcaccaatg attaagtgtt tgaaaaaacg tttatgaggc gtatcaaggc ccgcaatatt
    57181 gcaacaggca ggaacgaggg agaagagcag accgaatcgt aattcgccaa gcattaaccc
    57241 aacggccacg ggcaaacata gcaccagcgt ttgtcgaagt gcatagttga tatcggggtg
    57301 ataaatcagt ctgcgccaca tcgggggaaa caaaatggcg cgctaccagg taacgcgcca
    57361 ctccgacggg attaacgagt gccgtaaacg acgatggttt taccgtgtgc ggagatcagg
    57421 ttctgatctt ccagcatctt cagaatgcgt cccacggttt cacgagaaca gccgacaatc
    57481 tgaccgattt cctgacgggt aattttgatt tgcataccgt ccgggtgagt catagcatct
    57541 ggttgttttg ccaggttcag cagagtctgt gcaatgcggc ccgtcacgtc gaggaacgcc
    57601 aggttgccca ctttctctga agtgacttgc agacgacgcg ccatctgtgc agacagacgc
    57661 atcagaatgt ccgggtttac ctgaatcaat tggcgaaatt ttttgtacga aatttcagcc
    57721 acttcacagg cggttttcgc acgtacccat gcgctacgtt cctggccctc ttcaaacagg
    57781 cccagttcgc caataaaatc accctgattc agataggaga ggatcatttc tttaccctct
    57841 tcgtctttga tcagcactgc cacagagcct ttaacgatgt agtacagcgt ttccgctttt
    57901 tcaccctggt gaataagcgt gctcttggat gggtacttat gaatgtggca atgagacaag
    57961 aaccattcga gagtcgggtc tgtttgcggt ttgccaagca ccatgcgcgg ttatcctctg
    58021 ttataagctt tctccagagc cagataacgc cgctgtctct ggattgccga aatatgcttc
    58081 ccgctacctg ggaaggggct atcaactgta ctgcacggta atgtgacgtc ctttgcatac
    58141 atgcagtaca tcaatgtatt actgtagcat cctgactgtt ttagcatagc tttcgctttg
    58201 tgtctcctgg tgtctcgctt cagcatgacc caggtcgcct tccgttgcgc gatttggtta
    58261 gtacgcgtac tctgtcagga aaattgacgc agtggagtag caaaaatgca agcgcgagtg
    58321 aagtgggtcg aagggttaac ttttctgggc gaatccgcct ctggtcacca gattttaatg
    58381 gacggcaact caggcgataa agcaccaagt ccgatggaaa tggtgttgat ggcggcgggt
    58441 ggttgcagtg ccatcgatgt ggtttcgatc ctgcaaaaag ggcgtcagga tgtggtcgat
    58501 tgtgaagtaa aattgacctc tgaacgccgc gaagaggcac cacgcctgtt tacgcacatt
    58561 aatctgcatt ttatcgtcac cggtcgcgac ctgaaagacg cagcggttgc gcgtgcggtt
    58621 gatctctctg ccgagaaata ttgttcagtg gcgttgatgc tggaaaaagc ggtgaatatt
    58681 actcactcgt atgaagtggt tgccgcgtga cttaactatc ccgttgtgaa gccggatagt
    58741 attttatccg gcataatagc aaagttactc gattttcttt ccttccatca atcgttgcac
    58801 cagcggcagc ataattaatt ccattgccag ccccatttta ccgccaggta ccactaacgt
    58861 attgatgtgg gaaatgaatg agccttgcaa catcgccagc agccaaggga aatcgatccc
    58921 ttccagatta cgaaaatgga tcaccacaaa gctttcatcc agcgacggga taccttttgc
    58981 cgcgaacggg tttgaagtgt cgacggtggg aacgcgctgg aagttaagat gggtgcggga
    59041 aaactgcggt gtgatgtagt tgatatagtc ttccattgaa cgcactaccg agtccatcac
    59101 tgcttctcgt gagtgcccgc gctcgctggt gtcgcggatc agtttttgaa tccactcaag
    59161 gttaacgata ggcactacgc cgaccagtaa gtccacatgc tgcgcaacgt tatgctgtgg
    59221 cgtgactacg ccgccgtgta aaccttcata aaacagtaca tcagtgggtt ctggtaaagg
    59281 ttgccagggg gtgaatgttc ccggtacctg attccagggt acggcttcgt cgtaggtatg
    59341 cagatatttg cgagatttcc ctttgccgct ctgaccgtat tcaatgaagg tttgttccag
    59401 caggccgaag tcgttcgctt cggggccgaa gtagctgata tgccgcccgg cgtcgcgcgc
    59461 tttgcggatc gccatgtcca tttccgggcg ggtgtaacgg tgaaaactgt cgccttccac
    59521 ctcagctgca tgcagattta actgcgcgaa tattttacga aacgcgaggc tggtggtggt
    59581 ggtccccgcg ccgctggatc ctgttaccgc aatgaccgga tgtttggcag acatagtaac
    59641 tccatgactt agctatcact attatctcgt ccgccgacgg ttgcaggtta ttcgcgaaac
    59701 tgactgcgcg gcataatatt gaccgtttcg tgcagctccg accataccag caccgcttct
    59761 ccgcactgta gctggcgttt gacgtcggcg actttctgtt caagtgtacg ctcatgttca
    59821 ccataatcgg tgccttcacg taacacaaag ctttcaatta aattttccag cgtttcgggg
    59881 gagaggtctt gccacggaat caacatgatt tcgcctccag atacgttgtt aaccagtcag
    59941 gaatgcgtga ctccagccac atttgcggat gaagtaatgt accgccaata aagccaacat
    60001 gaccgccatg ttcagtcagt tgatactcca cctgcgaggg gagactttcc ggttttggga
    60061 tcacctgatg atccataaac ggatcgtctt tggcgtgaat aatcagcgtc ggtttggcga
    60121 tccggttcag cattggcatg gcgctacact gacgataata gtcgatagcg tcggcgtagc
    60181 cgtgaattct ggcggtgatt agatcgtcaa attcacggat gcgacgtacc gattttaact
    60241 gcgcgagatt aatcggcagc gttccggggt aggctgccag cttgcgcgcg gcattggctt
    60301 ttaacaggtt cagcaagtaa cgctgataaa cgcgggaaaa gcccttttcc atatgatagc
    60361 tacaggcttc cagcataaac ggcgcagaga caatcaccgc cgcatcaacc gggagatcat
    60421 tgccttcctt tgccagcaaa caggccagca tattaccgcc gagcgaatag ccgacggcag
    60481 ccgttggcgc atgaccaaat tcgcgttgca gccagcgtaa aaaccaactg gcgtcttcgg
    60541 tttcgcccga atggtaaatg cggtgcatac ggtttggttc accgctgcat ccgcgaaaat
    60601 gcatcaccac gcccagccag ccgcgctttt gcgccgcctc aaccagaccg tgggcgtaag
    60661 ggctattgag actgccttcc agcccgtgaa acaccactaa acgcggttta tgtttcgcct
    60721 gtgcggggtc ttcactccac gcgagatcga caaaatcgcc gtcgggcaac tccagccgct
    60781 gccagtacgg ggtgaatttc acctgacgac gaaacaaacg cggcagcata gtttgtagat
    60841 gacaattgct aaagccgcgc ataggggtga attcagcact gctgctaaat tcattggcat
    60901 cggtcgtcgt tatctgcgcc atcagttgct ttggccttcc agcagcatct gctcaagctg
    60961 ctcctgggct tccagccacg ccatttcgca ctcttccagg ccggatttgg cgctggcttg
    61021 ctgttgcagg caggcggtca actccgcttt acggctctgg tcatacagtt cgctgtcgcc
    61081 gagtttctct tccgcctgcg ccagttgcgc gttcagcttc tccatctctt tttccagacg
    61141 ggcaatctct ttacgcagcg gctgggtttg cgcacgcagc tccgcttccc gacgcttctg
    61201 atctttacgt gcctgggcgc tgttcgcgtt ctcttttggc gcttcgtcgg tctggttttc
    61261 ctgcttttgt acgtcgctca accactgttg ataatcttcc agatcgccgt cgaacggttc
    61321 gactttacga tcgtgaacca ggtagagatc gtcagtggtg gaacgcagca aatgacggtc
    61381 gtgcgaaacg acaaccagcg cgccttcaaa ctcgattaat gcttcggtga gtgcctgacg
    61441 catgtcgagg tcaaggtggt tagtcggttc gtcgagcagc agcagattcg g
//
LOCUS       DADLCQ010000007        55430 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000007 DADLCQ010000000
VERSION     DADLCQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.007"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQG95_000401"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JQG95_000401"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY5887085.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQG95_000402"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JQG95_000402"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY5887086.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQG95_000403"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JQG95_000403"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY5887087.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JQG95_000404"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JQG95_000404"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY5887088.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JQG95_000405"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JQG95_000405"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY5887089.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JQG95_000406"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JQG95_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY5887090.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JQG95_000407"
     CDS             9708..10559
                     /locus_tag="JQG95_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5887091.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSRCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JQG95_000408"
     CDS             complement(10546..10887)
                     /locus_tag="JQG95_000408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY5887092.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JQG95_000409"
     CDS             complement(10889..11767)
                     /locus_tag="JQG95_000409"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY5887093.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JQG95_000410"
     CDS             complement(11733..14030)
                     /locus_tag="JQG95_000410"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY5887094.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLGTYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JQG95_000411"
     CDS             complement(14081..14401)
                     /locus_tag="JQG95_000411"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY5887095.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JQG95_000412"
     CDS             complement(14416..15495)
                     /locus_tag="JQG95_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY5887096.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="JQG95_000413"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="JQG95_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JQG95_000414"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JQG95_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="JQG95_000415"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="JQG95_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20983
                     /locus_tag="JQG95_000416"
     CDS             20366..20983
                     /locus_tag="JQG95_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY5887097.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21010..21948)
                     /gene="yijE"
                     /locus_tag="JQG95_000417"
     CDS             complement(21010..21948)
                     /gene="yijE"
                     /locus_tag="JQG95_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY5887098.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22009..24189)
                     /gene="katG"
                     /locus_tag="JQG95_000418"
     CDS             complement(22009..24189)
                     /gene="katG"
                     /locus_tag="JQG95_000418"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY5887099.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24518..25408)
                     /gene="metF"
                     /locus_tag="JQG95_000419"
     CDS             complement(24518..25408)
                     /gene="metF"
                     /locus_tag="JQG95_000419"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY5887100.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25757..28189)
                     /locus_tag="JQG95_000420"
     CDS             complement(25757..28189)
                     /locus_tag="JQG95_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY5887101.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28192..29352)
                     /gene="metB"
                     /locus_tag="JQG95_000421"
     CDS             complement(28192..29352)
                     /gene="metB"
                     /locus_tag="JQG95_000421"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY5887102.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29629..29946
                     /gene="metJ"
                     /locus_tag="JQG95_000422"
     CDS             29629..29946
                     /gene="metJ"
                     /locus_tag="JQG95_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY5887103.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30130..30738
                     /locus_tag="JQG95_000423"
     CDS             30130..30738
                     /locus_tag="JQG95_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY5887104.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="JQG95_000424"
     CDS             complement(30799..31011)
                     /gene="rpmE"
                     /locus_tag="JQG95_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY5887105.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31214..33412
                     /gene="priA"
                     /locus_tag="JQG95_000425"
     CDS             31214..33412
                     /gene="priA"
                     /locus_tag="JQG95_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY5887106.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRMRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33568..34593
                     /gene="cytR"
                     /locus_tag="JQG95_000426"
     CDS             33568..34593
                     /gene="cytR"
                     /locus_tag="JQG95_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY5887107.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34685..35644
                     /gene="ftsN"
                     /locus_tag="JQG95_000427"
     CDS             34685..35644
                     /gene="ftsN"
                     /locus_tag="JQG95_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY5887108.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35737..36267
                     /gene="hslV"
                     /locus_tag="JQG95_000428"
     CDS             35737..36267
                     /gene="hslV"
                     /locus_tag="JQG95_000428"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY5887109.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36277..37608
                     /gene="hslU"
                     /locus_tag="JQG95_000429"
     CDS             36277..37608
                     /gene="hslU"
                     /locus_tag="JQG95_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY5887110.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37675..38601
                     /gene="menA"
                     /locus_tag="JQG95_000430"
     CDS             37675..38601
                     /gene="menA"
                     /locus_tag="JQG95_000430"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY5887111.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38694..39179
                     /gene="rraA"
                     /locus_tag="JQG95_000431"
     CDS             38694..39179
                     /gene="rraA"
                     /locus_tag="JQG95_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY5887112.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="JQG95_000432"
     CDS             complement(39264..39509)
                     /gene="zapB"
                     /locus_tag="JQG95_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY5887113.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39935..40780
                     /gene="glpF"
                     /locus_tag="JQG95_000433"
                     /pseudo
     CDS             39935..40780
                     /gene="glpF"
                     /locus_tag="JQG95_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40820..42310
                     /gene="glpK"
                     /locus_tag="JQG95_000434"
     CDS             40820..42310
                     /gene="glpK"
                     /locus_tag="JQG95_000434"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY5887114.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDVLGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42540..43550
                     /gene="glpX"
                     /locus_tag="JQG95_000435"
     CDS             42540..43550
                     /gene="glpX"
                     /locus_tag="JQG95_000435"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY5887115.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43647..44393
                     /gene="fpr"
                     /locus_tag="JQG95_000436"
     CDS             43647..44393
                     /gene="fpr"
                     /locus_tag="JQG95_000436"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY5887116.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="JQG95_000437"
     CDS             complement(44398..44826)
                     /gene="uspD"
                     /locus_tag="JQG95_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY5887117.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44853..45152)
                     /locus_tag="JQG95_000438"
     CDS             complement(44853..45152)
                     /locus_tag="JQG95_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY5887118.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45364..45804)
                     /locus_tag="JQG95_000439"
                     /pseudo
     CDS             complement(45364..45804)
                     /locus_tag="JQG95_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45905..46504
                     /locus_tag="JQG95_000440"
     CDS             45905..46504
                     /locus_tag="JQG95_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY5887119.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46612..47379
                     /gene="tpiA"
                     /locus_tag="JQG95_000441"
     CDS             46612..47379
                     /gene="tpiA"
                     /locus_tag="JQG95_000441"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY5887120.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="JQG95_000442"
     CDS             complement(47434..48189)
                     /gene="cdh"
                     /locus_tag="JQG95_000442"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY5887121.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48296..49285)
                     /locus_tag="JQG95_000443"
     CDS             complement(48296..49285)
                     /locus_tag="JQG95_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5887122.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     SHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="JQG95_000444"
     CDS             complement(49605..50567)
                     /gene="pfkA"
                     /locus_tag="JQG95_000444"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY5887123.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="JQG95_000445"
     CDS             complement(50748..51650)
                     /gene="fieF"
                     /locus_tag="JQG95_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY5887124.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="JQG95_000446"
     CDS             complement(51798..52298)
                     /gene="cpxP"
                     /locus_tag="JQG95_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY5887125.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52448..53146
                     /gene="cpxR"
                     /locus_tag="JQG95_000447"
     CDS             52448..53146
                     /gene="cpxR"
                     /locus_tag="JQG95_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY5887126.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53143..54516
                     /gene="cpxA"
                     /locus_tag="JQG95_000448"
     CDS             53143..54516
                     /gene="cpxA"
                     /locus_tag="JQG95_000448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY5887127.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="JQG95_000449"
     CDS             complement(54622..55296)
                     /gene="yiiM"
                     /locus_tag="JQG95_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY5887128.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13504 a        15021 c        14162 g        12743 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcagttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cgctgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggt gccaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcagcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgtgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtaccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgaaggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctgacagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatccg gcgtatatgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg
    20581 tgtatgttcc gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact
    20641 ggttcacgaa gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg
    20701 cccggacagc aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca
    20761 caacaaaaca cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc
    20821 ctggcgatta gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga
    20881 cggtacgccg ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt
    20941 tagctggcgg atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga
    21001 agaaaaaatt cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc
    21061 cagtgccagc acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag
    21121 cagccaccag gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat
    21181 actggcaggc aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag
    21241 aatcgcactg taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg
    21301 caccagtaaa gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa
    21361 cgataataaa tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc
    21421 cccccaacta acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag
    21481 ttgccacggc tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg
    21541 cccacgtcgc aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat
    21601 ggtatagctc aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccaa
    21661 accaaccatc ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg
    21721 gcgcattccg cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca
    21781 gcgtaaggcg gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca
    21841 gctataactc cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt
    21901 tcctgcggca gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta
    21961 aagaaacttc agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc
    22021 gaaacggtcg aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc
    22081 gtgggcatcg ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa
    22141 taccagatcc gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc
    22201 gaacagctct ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac
    22261 gaagaagtca ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc
    22321 gaagttggca cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag
    22381 cgtcagttgc tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc
    22441 gcgatagtta cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt
    22501 ctgatcctga cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc
    22561 ggcgcttgcg gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga
    22621 ggctttacca gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc
    22681 gttcacatcc cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg
    22741 tttgtcgcca ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac
    22801 agacagacca gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt
    22861 gtagatcggc tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc
    22921 gatgtagcga gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa
    22981 ggcttcgttg aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg
    23041 atcaaaacgc agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc
    23101 gaacggatcc gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg
    23161 actgcgggtc tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg
    23221 ggtcggcgtc tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc
    23281 gctgccgtaa gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc
    23341 aggacctacg tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc
    23401 aatcagcgcc acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc
    23461 tgctgccgca gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag
    23521 gcccatctcg gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag
    23581 ccaggctttt tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc
    23641 ggcaccaaaa ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat
    23701 aaacaggtcg gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg
    23761 acgcgcttta tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg
    23821 accacgaccc gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc
    23881 catacgaata aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc
    23941 tgtcagcagg gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt
    24001 gcggtagtca aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc
    24061 aacacggagt tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc
    24121 gtgaccgccc tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga
    24181 cgtgctcatc aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag
    24241 aagttataat tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa
    24301 agagatgtag atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa
    24361 atgctttata aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg
    24421 ctgacaacct tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt
    24481 tgtgatcact gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca
    24541 gcgtatggca aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt
    24601 tcactccttc acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt
    24661 tgcgggtttc ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac
    24721 gcacgttggt catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc
    24781 ccgggatgat ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc
    24841 tttcgacatc gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt
    24901 tcagattaag caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg
    24961 ccacggatat atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca
    25021 tttctggctt accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac
    25081 cgttattcca gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag
    25141 taagatgcgg tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg
    25201 tacggtcgcg ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc
    25261 tgctaaggcg atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga
    25321 aaaactcgaa cgaaacatta atctgcccct ggacttctgc caggctctga ttcagggcat
    25381 cccgctggct ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg
    25441 ttgtgttgtg cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa
    25501 gggcgtcaac cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact
    25561 tcaggatggt aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac
    25621 taaccatgta cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc
    25681 tatgataatt caatatattt cattagaaac catagacccg aaaacatgag taccgggcat
    25741 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
    25801 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc
    25861 agcggctttc gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt
    25921 cacgcaccgc ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc
    25981 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
    26041 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg
    26101 attccacacg tacctgatcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca
    26161 gcttgcgcat tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct
    26221 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
    26281 gccaggagag cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga
    26341 tcagatcgcg cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc
    26401 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg
    26461 ctcccgccag tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact
    26521 gatcagcaag ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg
    26581 cgcgcatcca caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg
    26641 ctaacgcgcg gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca
    26701 cgcctgccag cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga
    26761 acagttccag ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt
    26821 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
    26881 gcagtactgc caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc
    26941 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga
    27001 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt
    27061 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc
    27121 gatcgttatg cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga
    27181 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
    27241 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
    27301 gcgtggaacc ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag
    27361 aaactggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg
    27421 cctcatccag acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac
    27481 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
    27541 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt
    27601 tacggctgat aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt
    27661 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta
    27721 aaaactcacg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
    27781 tcaggcgcgc cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa
    27841 taccactgtc gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc
    27901 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
    27961 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
    28021 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact
    28081 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
    28141 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
    28201 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
    28261 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
    28321 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
    28381 cgccagcgta aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc
    28441 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
    28501 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
    28561 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
    28621 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
    28681 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
    28741 agagtgaccg ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag
    28801 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
    28861 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
    28921 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
    28981 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
    29041 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
    29101 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
    29161 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
    29221 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
    29281 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
    29341 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
    29401 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
    29461 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
    29521 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
    29581 attgtggggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
    29641 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
    29701 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
    29761 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
    29821 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
    29881 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
    29941 tactaaagcg gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc
    30001 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
    30061 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
    30121 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
    30181 gcctggcagc cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa
    30241 gcgatccaac tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac
    30301 aaaaagccct acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg
    30361 attgcccatg gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt
    30421 gaacaacagc aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
    30481 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
    30541 aacgcgctgg gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac
    30601 ccgctggtcc aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg
    30661 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg
    30721 ccgctgtttt cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct
    30781 tttttcggaa atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc
    30841 aacacggcca ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct
    30901 gcacacgtcg aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca
    30961 agagcagcta gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc
    31021 tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag
    31081 tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg
    31141 tatgcaaact gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag
    31201 tcaggatgat gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt
    31261 tgactatctg ctgccagaag gcatgacggt taaagctggg tgtcgcatgc gcgtgccgtt
    31321 tggcaaacag caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc
    31381 gctcactgag ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc
    31441 cgtctggcga ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct
    31501 gtttcatgcc ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg
    31561 gtactggttt gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc
    31621 caagcaacaa caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc
    31681 cacgctcgaa tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt
    31741 agcaagtgaa acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg
    31801 gttgcgattg aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac
    31861 tttttctgcc tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag
    31921 tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg
    31981 cctgacaccg caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct
    32041 gcattccggt ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga
    32101 ggcggcgatt gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt
    32161 gattgtcatt gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca
    32221 tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc
    32281 cgcaacgccc gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg
    32341 cctgacgcgc cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg
    32401 tcagaaggtg caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca
    32461 ggccaacaat caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg
    32521 ccacgactgc ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca
    32581 ggcgcaacag catttgcgct gtcaccactg tgacagtcag cgtccagtgc cgcgccagtg
    32641 cccttcctgc ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca
    32701 gacgcttgcg ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag
    32761 ccgcaaaggg gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt
    32821 gatcggcaca caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt
    32881 actggatgtg gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca
    32941 gctttacacc caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct
    33001 gcaaacgcac catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc
    33061 ctttgccgaa caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca
    33121 tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact
    33181 gcgtaatctg atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt
    33241 tccggcactg gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc
    33301 ttcccgcgtg cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc
    33361 ggattcccgc aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta
    33421 cgatgcgagg cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac
    33481 cgttttcacg cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac
    33541 gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc
    33601 atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg
    33661 aatcccgata aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg
    33721 ggttatttac cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg
    33781 gtgattgtcc cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt
    33841 acggcggcaa atcacggata tctggtgctg attggcgact gtgcacatca aaatcagcag
    33901 gaaaaaacct ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt
    33961 tcaaggctgc cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg
    34021 gcgaacgaat ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc
    34081 gcatttgatg cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc
    34141 ggtcccgaag agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt
    34201 cgctgcggaa ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc
    34261 ggaagcaaag caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc
    34321 catagcgatg tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc
    34381 ccggaagacc tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg
    34441 ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg
    34501 cttgatcaaa tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt
    34561 atcatccggg gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg
    34621 gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac
    34681 gatagtggca caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa
    34741 gagcacctca cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat
    34801 tgctgccgcc gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa
    34861 agaagagtcc gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc
    34921 agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc
    34981 cacagaacct tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg
    35041 tcagcttctt gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt
    35101 tccgtggaat gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc
    35161 gcagcaatta gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg
    35221 gcagcagcag acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa
    35281 accagcttct actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc
    35341 gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac
    35401 ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga
    35461 acaggcagag acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac
    35521 caacaatggc tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag
    35581 caccctcaat cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg
    35641 ttgaaaccct caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg
    35701 gtttgtactc tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc
    35761 cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg
    35821 aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg
    35881 ggcggtactg cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat
    35941 cagggccatc tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg
    36001 ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc
    36061 ggtaacggtg acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct
    36121 tacgcccagg ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt
    36181 gctgaaaagg cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc
    36241 atcgaagaat taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa
    36301 atcgtcagcg aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg
    36361 attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg
    36421 accccgaaaa atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt
    36481 cgtctggcta agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa
    36541 gtgggctacg tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg
    36601 aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa
    36661 gaacgtattc tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag
    36721 cagcaggaac cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt
    36781 gatgacaaag aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct
    36841 cctccgggca tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc
    36901 cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa
    36961 gaagcggcga aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag
    37021 cagcacggga tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc
    37081 ggtccggatg tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc
    37141 accgtttcca ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc
    37201 gcgttccaga ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc
    37261 cgcgttgaac tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat
    37321 gcctctatca ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt
    37381 accgactccg gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa
    37441 aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac
    37501 gacgccagcg atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat
    37561 ctggatgcgt tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc
    37621 attttcatca ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact
    37681 gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc
    37741 ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat
    37801 ccgctggtcg ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc
    37861 gccaatgatt acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta
    37921 cgcggcatgc aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc
    37981 gtcgtgctca tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc
    38041 gattttgtcg gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc
    38101 gtgggcaatc gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt
    38161 ggctggttga gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg
    38221 atccttccgg cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg
    38281 cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa
    38341 gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg
    38401 tttaatctct tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta
    38461 ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg
    38521 ctggaacgta ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc
    38581 ctaagccagt gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca
    38641 gcccacatag cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat
    38701 acgatacttc cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt
    38761 tctccaactt tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg
    38821 aggacaacgg gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg
    38881 tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag
    38941 tacaaaatga atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg
    39001 aagagttgga tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag
    39061 gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc
    39121 atctttatgc cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat
    39181 aagaaaggca ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa
    39241 attacgcgaa agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga
    39301 cgttcctgcc agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg
    39361 cgctgatgct gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct
    39421 tcgatttcca tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt
    39481 ttctcaaaca cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat
    39541 aaaaaatcct cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag
    39601 ctccgattgt atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt
    39661 gccatacttt tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa
    39721 tttgttgcgc gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg
    39781 cgataacgct cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg
    39841 ctcgttaacg ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg
    39901 catacaacaa acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg
    39961 ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt
    40021 tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg
    40081 actgggggtg gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc
    40141 cgctgttacc attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt
    40201 tatcgtttca caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta
    40261 caatttattt ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt
    40321 tgatctggct ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt
    40381 cgcagttgag atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga
    40441 tggcaacggt gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt
    40501 cattggcgca tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg
    40561 tccgaaagtc tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga
    40621 cattccttac ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt
    40681 tgcctaccgc aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa
    40741 ggaaaccaca actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa
    40801 acatgactga aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    40861 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    40921 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    40981 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    41041 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    41101 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    41161 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    41221 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    41281 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    41341 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    41401 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    41461 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    41521 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    41581 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    41641 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    41701 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    41761 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    41821 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    41881 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    41941 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    42001 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    42061 gcagttccag tccgatgttc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
    42121 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
    42181 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
    42241 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    42301 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
    42361 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
    42421 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
    42481 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    42541 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    42601 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    42661 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    42721 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    42781 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    42841 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    42901 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
    42961 ataacctgcg caatgtagca gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
    43021 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
    43081 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    43141 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
    43201 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    43261 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    43321 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
    43381 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
    43441 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
    43501 tccactatct ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat
    43561 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    43621 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    43681 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    43741 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    43801 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    43861 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
    43921 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
    43981 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    44041 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    44101 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    44161 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    44221 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    44281 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    44341 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    44401 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    44461 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
    44521 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    44581 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    44641 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    44701 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    44761 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    44821 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    44881 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    44941 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    45001 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    45061 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    45121 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    45181 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    45241 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    45301 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    45361 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
    45421 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    45481 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
    45541 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    45601 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
    45661 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
    45721 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    45781 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    45841 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    45901 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    45961 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    46021 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
    46081 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    46141 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    46201 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    46261 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    46321 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    46381 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
    46441 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    46501 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    46561 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    46621 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    46681 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    46741 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    46801 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    46861 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    46921 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
    46981 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
    47041 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    47101 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    47161 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    47221 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    47281 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    47341 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    47401 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
    47461 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
    47521 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    47581 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
    47641 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
    47701 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
    47761 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    47821 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    47881 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    47941 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    48001 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    48061 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    48121 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    48181 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    48241 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
    48301 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    48361 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    48421 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    48481 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    48541 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
    48601 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    48661 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    48721 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    48781 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
    48841 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    48901 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    48961 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
    49021 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    49081 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    49141 gtttccagtg ggagctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    49201 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    49261 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    49321 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    49381 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    49441 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    49501 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    49561 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    49621 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    49681 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    49741 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    49801 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    49861 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    49921 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    49981 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
    50041 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    50101 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    50161 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    50221 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    50281 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    50341 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
    50401 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
    50461 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    50521 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
    50581 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    50641 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    50701 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    50761 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
    50821 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    50881 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    50941 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    51001 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    51061 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    51121 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    51181 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    51241 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    51301 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    51361 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    51421 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    51481 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    51541 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    51601 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    51661 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    51721 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    51781 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    51841 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    51901 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    51961 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    52021 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    52081 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    52141 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    52201 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    52261 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    52321 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    52381 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    52441 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    52501 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    52561 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    52621 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    52681 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    52741 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc
    52801 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    52861 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    52921 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    52981 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    53041 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    53101 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    53161 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    53221 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    53281 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    53341 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    53401 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    53461 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    53521 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    53581 cgtccggcca gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    53641 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    53701 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    53761 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    53821 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    53881 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    53941 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    54001 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    54061 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    54121 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    54181 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    54241 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    54301 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    54361 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    54421 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    54481 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    54541 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    54601 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    54661 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    54721 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    54781 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    54841 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    54901 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    54961 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    55021 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    55081 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    55141 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    55201 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    55261 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    55321 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    55381 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DADLCQ010000008        53210 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000008 DADLCQ010000000
VERSION     DADLCQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.008"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(11..322)
                     /locus_tag="JQG95_000450"
     CDS             complement(11..322)
                     /locus_tag="JQG95_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY5887129.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JQG95_000451"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JQG95_000451"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JQG95_000452"
     CDS             701..1054
                     /locus_tag="JQG95_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY5887130.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQG95_000453"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JQG95_000453"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY5887131.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSHVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JQG95_000454"
     CDS             complement(2810..3280)
                     /locus_tag="JQG95_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887132.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQG95_000455"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JQG95_000455"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY5887133.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILANLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQG95_000456"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JQG95_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY5887134.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQG95_000457"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JQG95_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY5887135.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JQG95_000458"
     CDS             complement(5283..8432)
                     /locus_tag="JQG95_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY5887136.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRNNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQG95_000459"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JQG95_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY5887137.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JQG95_000460"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JQG95_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY5887138.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JQG95_000461"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JQG95_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY5887139.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQG95_000462"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JQG95_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY5887140.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQG95_000463"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JQG95_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY5887141.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHRELEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JQG95_000464"
     CDS             14911..15288
                     /locus_tag="JQG95_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY5887142.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JQG95_000465"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JQG95_000465"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY5887143.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JQG95_000466"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JQG95_000466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY5887144.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JQG95_000467"
     CDS             18099..18428
                     /locus_tag="JQG95_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY5887145.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JQG95_000468"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JQG95_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY5887146.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JQG95_000469"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JQG95_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY5887147.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JQG95_000470"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JQG95_000470"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY5887148.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JQG95_000471"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JQG95_000471"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY5887149.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQG95_000472"
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="JQG95_000472"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY5887150.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24044..25348
                     /locus_tag="JQG95_000473"
     CDS             24044..25348
                     /locus_tag="JQG95_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY5887151.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="JQG95_000474"
     CDS             complement(25481..27157)
                     /locus_tag="JQG95_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY5887152.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /locus_tag="JQG95_000475"
     CDS             complement(27395..28615)
                     /locus_tag="JQG95_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887153.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /locus_tag="JQG95_000476"
     CDS             28833..30485
                     /locus_tag="JQG95_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY5887154.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQG95_000477"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="JQG95_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY5887155.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="JQG95_000478"
     CDS             complement(31205..31999)
                     /locus_tag="JQG95_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887156.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="JQG95_000479"
     CDS             32137..32478
                     /locus_tag="JQG95_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY5887157.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQG95_000480"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="JQG95_000480"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY5887158.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAELQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="JQG95_000481"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="JQG95_000481"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY5887159.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="JQG95_000482"
     CDS             36394..37686
                     /locus_tag="JQG95_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5887160.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38245
                     /gene="cueR"
                     /locus_tag="JQG95_000483"
     CDS             37811..38245
                     /gene="cueR"
                     /locus_tag="JQG95_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HAY5887161.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAGGLRRDGSDM"
     gene            complement(38219..38677)
                     /locus_tag="JQG95_000484"
     CDS             complement(38219..38677)
                     /locus_tag="JQG95_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY5887162.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="JQG95_000485"
     CDS             complement(38674..39591)
                     /locus_tag="JQG95_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY5887163.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="JQG95_000486"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="JQG95_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY5887164.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="JQG95_000487"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="JQG95_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY5887165.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQG95_000488"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="JQG95_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY5887166.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLQDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQG95_000489"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="JQG95_000489"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY5887167.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQG95_000490"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="JQG95_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="JQG95_000491"
     CDS             43550..44236
                     /locus_tag="JQG95_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5887168.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46512
                     /locus_tag="JQG95_000492"
     CDS             44233..46512
                     /locus_tag="JQG95_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5887169.1"
                     /translation="MIARWFWREWSSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCDVRHAWVCFR
                     PGGRNWCGNGTGSVAGESV"
     gene            46490..46648
                     /locus_tag="JQG95_000493"
     CDS             46490..46648
                     /locus_tag="JQG95_000493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5887170.1"
                     /translation="MLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGK
                     ALFRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JQG95_000494"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JQG95_000494"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY5887171.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JQG95_000495"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JQG95_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY5887172.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JQG95_000496"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JQG95_000496"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY5887173.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JQG95_000497"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JQG95_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY5887174.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JQG95_000498"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JQG95_000498"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY5887175.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="JQG95_000499"
     CDS             52938..>53210
                     /locus_tag="JQG95_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY5887176.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12829 a        13690 c        14020 g        12671 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgtggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattattgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccaattc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cttggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tagaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagctccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggggatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtcttgcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggagttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DADLCQ010000009        52075 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000009 DADLCQ010000000
VERSION     DADLCQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.009"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(262..2541)
                     /gene="maeB"
                     /locus_tag="JQG95_000500"
     CDS             complement(262..2541)
                     /gene="maeB"
                     /locus_tag="JQG95_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY5887177.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2830..3780
                     /gene="tal"
                     /locus_tag="JQG95_000501"
     CDS             2830..3780
                     /gene="tal"
                     /locus_tag="JQG95_000501"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY5887178.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3800..5803
                     /gene="tkt"
                     /locus_tag="JQG95_000502"
     CDS             3800..5803
                     /gene="tkt"
                     /locus_tag="JQG95_000502"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY5887179.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTAQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5906..6949)
                     /locus_tag="JQG95_000503"
     CDS             complement(5906..6949)
                     /locus_tag="JQG95_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY5887180.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="JQG95_000504"
     CDS             complement(7075..7650)
                     /gene="nudK"
                     /locus_tag="JQG95_000504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY5887181.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="JQG95_000505"
     CDS             complement(7718..9697)
                     /gene="aegA"
                     /locus_tag="JQG95_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY5887182.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVLIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9903..11620
                     /gene="narQ"
                     /locus_tag="JQG95_000506"
                     /pseudo
     CDS             9903..11620
                     /gene="narQ"
                     /locus_tag="JQG95_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            11822..14935
                     /gene="acrD"
                     /locus_tag="JQG95_000507"
     CDS             11822..14935
                     /gene="acrD"
                     /locus_tag="JQG95_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY5887183.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="JQG95_000508"
     CDS             complement(15034..15093)
                     /gene="ypfM"
                     /locus_tag="JQG95_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY5887184.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            15474..15830
                     /locus_tag="JQG95_000509"
     CDS             15474..15830
                     /locus_tag="JQG95_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY5887185.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            15834..16961
                     /gene="dapE"
                     /locus_tag="JQG95_000510"
     CDS             15834..16961
                     /gene="dapE"
                     /locus_tag="JQG95_000510"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY5887186.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            16989..17189
                     /locus_tag="JQG95_000511"
     CDS             16989..17189
                     /locus_tag="JQG95_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY5887187.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="JQG95_000512"
     CDS             complement(17299..17997)
                     /gene="ypfH"
                     /locus_tag="JQG95_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5887188.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(18071..20086)
                     /locus_tag="JQG95_000513"
     CDS             complement(18071..20086)
                     /locus_tag="JQG95_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY5887189.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(20101..20964)
                     /locus_tag="JQG95_000514"
     CDS             complement(20101..20964)
                     /locus_tag="JQG95_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY5887190.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(21132..21845)
                     /gene="purC"
                     /locus_tag="JQG95_000515"
     CDS             complement(21132..21845)
                     /gene="purC"
                     /locus_tag="JQG95_000515"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY5887191.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="JQG95_000516"
     CDS             complement(22058..23092)
                     /gene="bamC"
                     /locus_tag="JQG95_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY5887192.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="JQG95_000517"
     CDS             complement(23109..23987)
                     /gene="dapA"
                     /locus_tag="JQG95_000517"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY5887193.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            24133..24705
                     /locus_tag="JQG95_000518"
     CDS             24133..24705
                     /locus_tag="JQG95_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY5887194.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            24705..25175
                     /gene="bcp"
                     /locus_tag="JQG95_000519"
     CDS             24705..25175
                     /gene="bcp"
                     /locus_tag="JQG95_000519"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY5887195.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            25428..26045
                     /gene="hyfA"
                     /locus_tag="JQG95_000520"
     CDS             25428..26045
                     /gene="hyfA"
                     /locus_tag="JQG95_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY5887196.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            26045..28062
                     /gene="hyfB"
                     /locus_tag="JQG95_000521"
                     /pseudo
     CDS             26045..28062
                     /gene="hyfB"
                     /locus_tag="JQG95_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            28073..29020
                     /gene="hyfC"
                     /locus_tag="JQG95_000522"
     CDS             28073..29020
                     /gene="hyfC"
                     /locus_tag="JQG95_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY5887197.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            29037..30476
                     /locus_tag="JQG95_000523"
     CDS             29037..30476
                     /locus_tag="JQG95_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY5887198.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVVSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMTLLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            30488..31138
                     /gene="hyfE"
                     /locus_tag="JQG95_000524"
     CDS             30488..31138
                     /gene="hyfE"
                     /locus_tag="JQG95_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY5887199.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            31143..32729
                     /gene="hyfF"
                     /locus_tag="JQG95_000525"
     CDS             31143..32729
                     /gene="hyfF"
                     /locus_tag="JQG95_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY5887200.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            32719..34433
                     /gene="hyfG"
                     /locus_tag="JQG95_000526"
                     /pseudo
     CDS             32719..34433
                     /gene="hyfG"
                     /locus_tag="JQG95_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            34443..34988
                     /gene="hyfH"
                     /locus_tag="JQG95_000527"
     CDS             34443..34988
                     /gene="hyfH"
                     /locus_tag="JQG95_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY5887201.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            34985..35743
                     /gene="hyfI"
                     /locus_tag="JQG95_000528"
     CDS             34985..35743
                     /gene="hyfI"
                     /locus_tag="JQG95_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY5887202.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGTVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            35736..36149
                     /gene="hyfJ"
                     /locus_tag="JQG95_000529"
     CDS             35736..36149
                     /gene="hyfJ"
                     /locus_tag="JQG95_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY5887203.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            36179..38191
                     /locus_tag="JQG95_000530"
     CDS             36179..38191
                     /locus_tag="JQG95_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY5887204.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWQARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLSSVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            38213..39061
                     /locus_tag="JQG95_000531"
                     /pseudo
     CDS             38213..39061
                     /locus_tag="JQG95_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(39099..40160)
                     /locus_tag="JQG95_000532"
     CDS             complement(39099..40160)
                     /locus_tag="JQG95_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY5887205.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            40373..41836
                     /gene="bepA"
                     /locus_tag="JQG95_000533"
     CDS             40373..41836
                     /gene="bepA"
                     /locus_tag="JQG95_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY5887206.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            41857..42216
                     /gene="arsC"
                     /locus_tag="JQG95_000534"
     CDS             41857..42216
                     /gene="arsC"
                     /locus_tag="JQG95_000534"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY5887207.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(42349..43095)
                     /gene="hda"
                     /locus_tag="JQG95_000535"
     CDS             complement(42349..43095)
                     /gene="hda"
                     /locus_tag="JQG95_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY5887208.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="JQG95_000536"
     CDS             complement(43145..44434)
                     /gene="uraA"
                     /locus_tag="JQG95_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY5887209.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(44520..45146)
                     /gene="upp"
                     /locus_tag="JQG95_000537"
     CDS             complement(44520..45146)
                     /gene="upp"
                     /locus_tag="JQG95_000537"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY5887210.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            45471..46508
                     /gene="purM"
                     /locus_tag="JQG95_000538"
     CDS             45471..46508
                     /gene="purM"
                     /locus_tag="JQG95_000538"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY5887211.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            46508..47146
                     /gene="purN"
                     /locus_tag="JQG95_000539"
     CDS             46508..47146
                     /gene="purN"
                     /locus_tag="JQG95_000539"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY5887212.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            47318..49384
                     /gene="ppk1"
                     /locus_tag="JQG95_000540"
     CDS             47318..49384
                     /gene="ppk1"
                     /locus_tag="JQG95_000540"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY5887213.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            49389..50930
                     /gene="ppx"
                     /locus_tag="JQG95_000541"
     CDS             49389..50930
                     /gene="ppx"
                     /locus_tag="JQG95_000541"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY5887214.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="JQG95_000542"
     CDS             complement(50969..>52075)
                     /gene="pdeF"
                     /locus_tag="JQG95_000542"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY5887215.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        12155 a        13739 c        13522 g        12659 t
ORIGIN      
        1 cgtgacaatt tttcgtcaga gtaaataaca aaattcggca agcgcgttta aaaggtgagg
       61 tagatcaata agaatggaga gatatttact gttatgaata aactttcata tgcgacgcat
      121 gaatataaat aaaataatat cgagatattt tatatactga tttttttatt attcgcaaac
      181 tcagtaataa gctttgctaa tatatttcag gaaaaccgct taccctgaat attcagggta
      241 agcgtgagag ttaagaatga attacagcgg ttgggtttgc gcttcaacca cggccagcgc
      301 caccatattg acgatacgac gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc
      361 cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc gcagtaagtt
      421 gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattggcag aacctttcaa
      481 agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat
      541 ttcaccatca atcatcagat ctggcgcacg atccctgacc agttccagcg cctgacgcat
      601 tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca gagcaacgcg
      661 cggctcaata ccaaaacgac ggacagtttc tgccgccatc aaggtgatct ccgccagctc
      721 ttctgcgtct ggttcgtcat tgacgtaggt atcggcaata aaggtgttac cactcggcag
      781 cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa agacattttt
      841 caccacgcta aaatgttcat gataatcgcc caccgtaccg cagatcattg catcggcttc
      901 cccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc
      961 ctgttcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc agtactcttt
     1021 aaagcgcgga tcggattcgt tattgacgat ttcaaaatca acgcccgctt tgatctgcaa
     1081 gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccgataagga tcggtttcgc
     1141 cagtcccagc gttaccagtt cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg
     1201 cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca taaacaggtt
     1261 ggttttgtaa acgaactcag tcagcttgtc gatgtagacg tcgaaatcag caatcggacg
     1321 cgtcgccacg cccgactcca tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa
     1381 gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat cctgaacgcc
     1441 atacgctgaa gccaccactt cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac
     1501 cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg cgccacggaa
     1561 gatgaacggg aagcaaagaa cgttgttcac ctggttcggg tagtcggaac ggccggtaca
     1621 gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg gttccgggtt
     1681 cgccagcgcc aggatcattg gtgcccgagc cattttcttc accatttcct gggtcagcac
     1741 tttcgggccg gaacagccca ggaaaatatc cgcaccttca atcacatcat cgagggtacg
     1801 tttgccgtca tccaccaccg catatgcggc tttggtttcc gccatatttg gctcacggcc
     1861 ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca gccccagcgc
     1921 taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccggaaacca ccatccgcac
     1981 gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag tgctgatgat
     2041 tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc gcagtttctg
     2101 ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg
     2161 ttcgagcgcg gcgacgactt caataaattt gtccgggtcg agttcatcaa cttcaatgtc
     2221 aaatacatca atcccagcga atttcttaaa tagaacgccc ttgccttcca tcaccggttt
     2281 gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc
     2341 aaccaggtta ccgcgcgcgg tatatttgta ggcttttagc gggtcttttt cgatttcaag
     2401 acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg
     2461 cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat caagtgcact
     2521 ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca aataaagtgt
     2581 gtgggcaaca gcccctgccc acaacgtggc gcacattatt accctgccgg agtctacaga
     2641 ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat cctataacac
     2701 tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt
     2761 tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac ttttcaagga
     2821 gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt ggcagacagc
     2881 tgcgatattg agtccattcg ccattatcat ccccaggatg ccaccactaa tccttcgctg
     2941 ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc tatcgcctgg
     3001 ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga caaactggcg
     3061 gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat
     3121 gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct ggtggacttg
     3181 tatcagcaac aaggcgttga gaaatcacgc attttgatca aactggcttc gacctgggaa
     3241 ggaattcgcg cggccgaaga gctggaaaaa gaaggtatta actgcaacct gacgctgctg
     3301 ttttctttta cccaggcgcg ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt
     3361 gtagggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta tgtggtggaa
     3421 gaagatccgg gcgttaaatc ggtgcgcaat atctacgact actataagca acaccactat
     3481 gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc
     3541 tgcgatcgac tgactatcgc accgaatttg ctgaaggagc tgcaggaaaa agtttctcca
     3601 gtggtacgta aattaatccc gccttctcag acgttcccac gcccagcacc catgagcgaa
     3661 gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact gtctgaaggc
     3721 attcgtctgt tcgctgttga tcaacgcaaa ctggaagatc ttcttgccgc caaactataa
     3781 accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag
     3841 tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg gcatggctga
     3901 tattgccgaa gtgctgtgga acgattttct taaacataat cctaccgacc caacctggta
     3961 tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct acagtttgct
     4021 acatctgacc ggttacgacc tgccgctgga agaactgaag aacttccgtc agttgcattc
     4081 gaaaacccca ggccacccgg agattggcta tacgccaggc gttgaaacca ccaccggccc
     4141 gcttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta cactggcggc
     4201 gcagtttaac cagccagacc atgagatcgt cgatcacttc acctatgtgt ttatgggcga
     4261 cggctgcctg atggaaggta tttcccacga agtctgttcg ctggcaggca cgctgggact
     4321 aggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg aaacagaagg
     4381 ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg tgatccatga
     4441 aatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa
     4501 agataagccg tcgctgatta tctgccgtac ggtgattggc tttggttcgc cgaataaagc
     4561 aggtaaggaa gaggcgcacg gcgcaccact gggggaagaa gaagtggcgc tggcacggca
     4621 aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc acgcctggga
     4681 tgcccgtgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtttg ccgcctataa
     4741 aaaggctcat ccgcaactgg cagaagagtt tccccgacgg atgagcggtg gtttaccgaa
     4801 ggactgggag aaaacggctc agaaatatat caatgagtta caggcaaatc cggcgaaaat
     4861 cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc tgcctgagtt
     4921 gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag gttctgtttc
     4981 gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat ttggcatgac
     5041 cgctatcgcc aacggcatcg cgcaccacgg cggctttgtg ccgtataccg cgacgttcct
     5101 gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga aagcgcggca
     5161 gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccga cgcaccaggc
     5221 tgttgagcaa ctggccagcc tgcgcttaac gccaaatttc agcacctggc gaccgtgcga
     5281 tcagatggaa gcggcggtgg gctggaagct ggcggttgag cgccacaacg gaccgacggc
     5341 actgatcctc tcaaggcaga atctggccca ggtggaacgt acgccggatc aggttaaaga
     5401 gattgctcgt ggcggttatg tgctgaaaga cagcggcggt aagccagata ttattctgat
     5461 tgccaccggt tcagagatgg aaattaccct gcaagcggca gagaaattag caggagaagg
     5521 tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc aggatgagga
     5581 atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat
     5641 tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga tgacgggtta
     5701 cggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg ccgagaatat
     5761 tgtggcaaaa gcgcataagg tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc
     5821 tgtatgttcc cctcacccta accctctccc cagaagggcg aggggacggt ccgtgcgctt
     5881 tttagggccg catctgcgat tttgtttaac gggtgatcca caacgtgggc ctggcgtctg
     5941 gcccatgcca gttatcgcag gtaggttctg cggcgtaacg caccaggcga aaacgctgac
     6001 cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg
     6061 tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat cagttccagt tcattcgtct
     6121 cctggccgtt gttgaacggc aaacgcaacc gaaccgggcg cgaggctagc ggctttttac
     6181 gcgacacaat ccatgccaaa tcaatggtgt tataggcccc tgcctcacag ctaatcatca
     6241 tcagtgcttt atcatcggtc agcgcagtga cattcacctc acgacgtaac ggatcaagcg
     6301 agcagcgcag accattcatc cgccagtttc cataatccag caaatcgtta cgttcttcga
     6361 gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg
     6421 gtacgctgag aggcggttcg tcccctttct tgatccacgc ggtttcactg ccaacgcgct
     6481 tttgctgagc atcaataaac aacaacgccg cttttaagcc gctcagagaa atggtctgat
     6541 tgccgtcgcg taaggtgatc gccttccctt cctgaattat ctgcaaaaac gcggtgatgg
     6601 ttgccgtatc atcggttact aataaccatg gtgaaatccg ccacttgtca ccacttaatg
     6661 ccaacggctc accatctaat agcagccgtg gcgctatctc cccttcagac gcatccggcg
     6721 acttcaaccc accgcgctca atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc
     6781 tcagggtcat caccagtcca ttatgatcgc ccgtattgcg cgccacacaa aaattttgat
     6841 tattacaggt gacctgccag tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag
     6901 acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga
     6961 ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca
     7021 atcggatttg tcggattgag ccaataataa acaacgaaat attattgcta tttttcagtc
     7081 cattaaatgt gacatttgca aatagttaag caataacacc gtcttaccgt cacgtatctc
     7141 gccggttttg atcatctcca gcgcctggct gaacggcagc tcgagcactt caatatcttc
     7201 atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa
     7261 gtggattagc tcagtcacac cgccaggcga catatacagt tcaaataatt tgcgcacttc
     7321 gcccacttca tagcccgtct cttcaatcgc ttctttacga atgcacactt ccggttcgtc
     7381 gttatccagc agcccggcgc aggtttcaat cagctgcccg ctttcattgc cattaaccca
     7441 ggtagcgaca cggaactgac gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag
     7501 gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta tggcggataa cttcgccatc
     7561 tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa tagttatcgg agagaatttt
     7621 gtctttaatg agggtgattt gttgcgtcat accgactcca cagcgcgaaa tgaacaatta
     7681 tattacgctg tgaagtcggg tttgtctgcg caggctatca gtgagatttg actgatttta
     7741 cccccagcca gtcaataatc ccctgtgccg catgacgtcc ttctgccatt gcggtaacca
     7801 ccagatccgc accacgcacg gcgtcgccac cggcgaagat tttcggattg gtggtctggt
     7861 aacggtactg gctttccaca tccgcgatga tgcggcccca tttgtctacc gttacaccgt
     7921 gcgactccag ccacggcatc ccgtgtggat tgaagccaaa cgccataatc accgcatcgg
     7981 ctggcatgac aaattcactg ccctcaatca gtacaggtcg ccgacgcccc tgggcatccg
     8041 gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct
     8101 caagcgccac cggctggacg ttaaattcga agttggcccc ctcttcgcgg gcgttcttca
     8161 cctctttctt cgagcctggc atgttagctt catcacgacg ataagcgcag gtgacgttac
     8221 tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta
     8281 ccacgacgtt aagtccggcg gtattgataa acggctcttc cggtagctct tcgagcccca
     8341 tcacctgttt agtgttggca atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt
     8401 cattgggtaa acccgctttc atggaacggt aagtgcctac gccaacgaag accgcgtcgt
     8461 attgttccaa aagcaaatcc aaagagacat ctttacccac ttcacaattg agttcgaagt
     8521 gaatccccat cgcgctgaag atttcccgac ggcgtgccag cagggattta tccagtttga
     8581 aagaaggaat gccgaaagtg agcaagccac cgatttctgg atggcgatcg tacaccgtca
     8641 ccgccacgcc attgcgggtc agaacatccg cacaggccag ccctgccgga cctgccccga
     8701 taatcgccac ccgcttgtcc actttggtga catgacttaa gtcaggacgc caacctttcg
     8761 ccaacgcctg atctgaaata tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat
     8821 cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt
     8881 ccggcagggt gttggtctgg tgagaaagct cgacggcggc gtcgatgttt ccggctttca
     8941 ccagttcaat ccactgcgga atatggttat gcagcgggca ggtccattca caaacgctgt
     9001 gctcgccgaa cttaaggcag cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca
     9061 gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac
     9121 gcgcgggcgt tgcctgcatt tgttcgactt tactcattac cggcatttct tgtgcagcgg
     9181 tactggcatg ccacggttga tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca
     9241 tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac
     9301 aggcaggacc gttttcgcgc cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt
     9361 ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc aaacggacag gccaccacgc
     9421 aggatttaca gccaatacac ttttgctgat tgacctgaat gctgtcatca acgtggctga
     9481 ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc acaatggtga caggtcactg
     9541 cactacgttg ctgctgatgt ttgataaccg taattcgggg atgaaaatgg tgttggctca
     9601 ggacatgttg ctcatcattg tgagccatga cacaggcgat ttcacaagca tgacaaccca
     9661 gacattgctg actgttggcc ataataaaac gattcataac gaccttcttt tttggttgta
     9721 aaaaccttat tctttatatg agtgttgtta ttacccgact tacaggggaa ccggcaatgt
     9781 tcatgtgccc agaataagta actatttcgc tataaactgt ggcagatcaa ataatcccat
     9841 cactgactaa attgcgtttc agcgaactgg aacattaatg attttttgtg gagaagacgc
     9901 gtgtgattgt taaacgaccc gtctcggcca gtctggcccg ggcctttttt tacattgtgc
     9961 tgctgtcgat tctttccacg ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg
    10021 ctgaggctat caatattgcc ggatcgctgc gtatgcagag ttaccgcctg ggctacgact
    10081 tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt gtttcagcag gcactgcatt
    10141 caccggtatt aaccaacctc aacgtctggt atgtgccaga agcagtaaaa actcgctatg
    10201 cgcatctgaa tgccaactgg ctggagatga ataatcggct cagcaagggc gatttgccgt
    10261 ggtatcaggc caatattaat aattatgtta atcagataga cctgttcgtg ctggctttac
    10321 agcactacgc tgaacgcaaa atgctgctgg tggtggcgat ttccctggct ggcggtatcg
    10381 gtattttcac gctggtcttt tttactttgc gccgcatacg ccatcaggtg gttgccccgc
    10441 tgaatcagct ggttaccgcc agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc
    10501 tggataccag tctgccgaat gagcttggtc tgcttgcaaa aacctttaac caaatgtcga
    10561 gcgagctgca taaattgtac ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc
    10621 tccacgagac caagcgtcgt ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa
    10681 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    10741 agcagctgaa tatctggagc tgaacgttgg ggataactgg cggattagcg aagggaagcc
    10801 aaaccctgag ctgccaatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    10861 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    10921 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    10981 gatggaagaa cgtgcgacca tcgcccgcga attgcacgac tcgctggctc aggtgctttc
    11041 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    11101 acaaagtatc atggccgatt ttttccaggc gttgaatgat gcttatcggc agttacgcga
    11161 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    11221 aatgctggat acgttacaaa atcaaaccag cgctaaactg accctcgact gccgtctgcc
    11281 aaccctggca ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    11341 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    11401 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    11461 acccgaaggt cattatggac tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac
    11521 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctccgc
    11581 agagggtgag gggaagggga acacgccggc tgaggtgtaa ataattaccc tcacaccggc
    11641 cctctccctt taaaggcgag agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt
    11701 cagcaatctc atgataattt acattaactc ctttttttct ccacgattgg ctcgtacctt
    11761 gccgctacag tgaagcaagt caagcctaca acaatacgca gaaacacgag gtcctctttt
    11821 aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg
    11881 tctgacaggt accctggcga ttttttcatt gcccgttgaa caataccccg atctcgcgcc
    11941 accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc tggaaaacac
    12001 cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctgatgt atatgtcatc
    12061 tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag gcaccgatcc
    12121 ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa agttaccgca
    12181 ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaaca ttctgaccat
    12241 tgccttcgtc tctaccgatg gctcgatgga taaacaggat attgctgatt atgttgccag
    12301 taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc
    12361 gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc agatgacggc
    12421 taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg
    12481 tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc agtcactgct
    12541 gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaggacg gctcagaggt
    12601 aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt atcttagccg
    12661 cttcaatggt aagccagcct ccgggttggg ggtaaaactg gcctccagcg caaacgaaat
    12721 ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg
    12781 actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt
    12841 ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc tgttcctgca
    12901 aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga tgggaacctt
    12961 ctccgtactt tacgccttcg gttacagcgt caacacctta accatgttcg cgatggtgct
    13021 ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg aacgtattat
    13081 gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg
    13141 ggcactggtc gggattgcga tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg
    13201 cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg cgatggtgct
    13261 gtcagtactg gtagcgatgc tcctcactcc ggctctgtgt gccacgctac ttaagccact
    13321 gaaaaaaggt gagcatcacg ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa
    13381 ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg
    13441 gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc gtttgccgac
    13501 gtcgttctta ccgctggaag accggggcat gtttactacc tcggtacaat tgcccagcgg
    13561 ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca
    13621 tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg ggggtaacgg
    13681 gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg acagtaagac
    13741 cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc
    13801 tcgcgttatc gccagcagcc caccagcaat tagcggtctt ggtagttctg caggttttga
    13861 tatggagttg caggaccacg ctggagcggg tcacgatgcg ctgatggcag cacgtaatca
    13921 gttgctggcg ctggcggcgg aaaacccgga gctaacccgt gtgcgccata acggcctcga
    13981 tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga
    14041 tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg tgaatgactt
    14101 tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct
    14161 gccagatgac atcaatctct ggtatgtccg aaataaagat ggcggcatgg tgcccttctc
    14221 tgctttcgcg acctcacgct gggaaacagg ctcgccgcgt ctggaacgct ataacggtta
    14281 ttctgcggtt gagattgttg gggaagccgc accgggggtc agtaccggta cggcgatgga
    14341 tattatggaa tcgttagtga agcagctgcc aaacggcttt ggtctggagt ggacggcgat
    14401 gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca tttccttgct
    14461 ggtggtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt tctcggtaat
    14521 gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc gcgggctgga
    14581 aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc
    14641 catcctgatc gtcgagtttg ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc
    14701 gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc tggcatttat
    14761 cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc agcatgcggt
    14821 gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt acttcgtgcc
    14881 gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg aataagcaat
    14941 aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga acctgctcag
    15001 cgggttcttg ctgtttatac tttgtctcag gaattactta cgaagcataa cttcgataaa
    15061 gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa ttatgggtat
    15121 tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga tgactacctc
    15181 cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta tgtgatacac
    15241 agcaacattt cgagatattc atacggcatc taatactgat ttaattctgg ttaaaataca
    15301 gacagataac aagatgaata ttcttaatgt ttacgttaaa aatgtttaat attatttaat
    15361 agttgttaat ttgaatactt cgataatgtt atattttctg ataatcattt gcaggcaaaa
    15421 tgttttcacc cttaaatgag tatttattct cataaatcga aaaaggattc attatggtta
    15481 cactttacgg catcaaaaat tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca
    15541 ataacatcga ctatcgtttt catgattacc gcgtcgatgg gctggacagc gaattattga
    15601 acggttttat caacgaatta ggctgggaag cgttactcaa cacccgtggt acaacctggc
    15661 gtaaactgga cgaaactacc cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa
    15721 tgactgaaat gcctgcaatt atcaaacgtc cattgctctg cgcgcctggt aagcctatgc
    15781 tgctgggttt cagtgattcc agttatcagc aatttttcca tgaggtgtag tctatgtcgt
    15841 gcccggttat tgagctgaca caacagctta ttcgccgccc ttccctgagt cctgatgacg
    15901 caggatgtca ggctttgttg attgaacgtt tgcaggcgat cggttttacc gttgaacgca
    15961 tggattttgc cgatacgcag aatttttggg catggcgtgg gcaaggtgaa acgttagcct
    16021 ttgccgggca taccgacgtg gtgccgcctg gcgacgccga tcgttggatc aatccgccgt
    16081 ttgaacccac cattcgtgac ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct
    16141 cgctggcggc gatggtggta gcagcagaac gttttgtcgc acaacatccc aaccatacgg
    16201 ggcgactggc atttctgatc acttctgatg aagaagccag tgcccacaac ggtacggtaa
    16261 aagtcgtcga ggcgttaatg gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac
    16321 cgtcgagtat cgaagtggta ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct
    16381 gcaacctcac cattcatggc gttcaggggc atgttgccta cccacatctg gctgacaatc
    16441 cggtacatcg cgcagcacct ttccttaatg aattagtggc tattgagtgg gatcagggca
    16501 atgaattctt cccggcgacc agtatgcaga ttgccaatat tcaggcggga acgggcagta
    16561 acaatgttat tccgggtgaa ctgtttgtgc agtttaactt ccgcttcagc accgaactga
    16621 ctgatgagat gatcaaagcg caggtacttg ccctgcttga aaaacatcaa ctgcgctata
    16681 cggtggattg gtggctttcc gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg
    16741 cggtcgttaa cgcggttgag cactataatg aaattaaacc gcagctactg accacaggcg
    16801 gaacgtccga cgggcgcttt attgctcgca tgggggcgca ggtggtggaa ctcgggccgg
    16861 tcaatgccac tattcataaa attaatgaat gtgtgaacgc tgccgacctg cagctacttg
    16921 cccgtatgta tcaacgtatc atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg
    16981 aaataagcat ggactggctg gctaaatatt ggtggattct ggtgattgtc tttttggtag
    17041 gcgtcctgct gaacgtgatt aaagatctca agcgcgtcga ccataagaaa tttctcgcca
    17101 acaagccgga gcttcccccg catcgtgatt tcaacgataa gtgggacgat gacgacgact
    17161 ggccgaaaaa ggatcaaccg aagaagtaag ctgagtgctc aaccgtaggc cggataaggc
    17221 gttaacgccg catccggcaa tggtgaacga tccctgatgc gacgctcacg cgtcttatca
    17281 ggccaggtaa tctgtctttt acatcatttc aatcacatca tcatcgccag gcttaccgcc
    17341 acttagcgct tggtcgaaat aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa
    17401 ttgcatactg cgattgtcga tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc
    17461 accaccggca ctgattaacg cttcctgcgc cgcaactgca tgcgccagat cgataaccgg
    17521 atcttcacca ccatgaatca agtgaattgt cgtggctgta gacgcggtct ccggcaggct
    17581 cgcatagcgt ccgttaaagg caatgacgcg cgaagcaagg cccggttcgg ctttaatgct
    17641 ctccagcgcc ataatcgcgc cttgtgagaa accaattagc gccgtggcgt tagcccctac
    17701 tccgctctgt ttctgccagt agcgtaccgt ttcaataaac gtcggcatga tggcatccac
    17761 acgcgcctgg cgattatctt ccgtaatacc ctgcactgaa aaccactgac gccccgccgg
    17821 attaccgctc ggctccgcgc caccgacact caccaccagc gcatcaggaa acagcggtgc
    17881 aaaccagctg cctatttccc ccattgccac cgggttatcc ccgacaccgt ggaaaagcag
    17941 taacaactgt tgtgcaggtt tatccgggct ttgaacaaca aaatggtcat gtttcatggc
    18001 gaactcctta actgatgtca ttaattttac gccgctgact cacaatgacc atgggagaaa
    18061 attgaaggag tcagtgaaaa aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc
    18121 gaacctcatt tagtgcgaac agcgcctgcg cggcctcttc ccgctggcga accagtaaca
    18181 tcttgcggcc tgaaagttta agtgtggtac ataactgcgc gtcactggcg tttttctgta
    18241 aacgcccacg cagcgccggt aatgccagtt cactggtttg tagcagacgc aataagcaac
    18301 ctaacgatgt taatagcgga cgatgagcga aagcaaaacc ggccagttca agccagtcgt
    18361 cgtcagaaag gatggcatcg tttagtggat caacaggaag catttcgcca ttccactgcg
    18421 cgagagcttg cgcatcgcga cgtaaacggt aatgctcacg ttcagccagc tgtttacccg
    18481 catcactcat cggtaacagc gccatcgccg tatagcaacc gctgctggct tcccgatgat
    18541 tacccatccg caccagcaca aaaccgcagc gttgccagaa acgccataac tccccagtgt
    18601 aaccaaaact caccgaaaga tagtcgaggt catgcgtata ttgcaaagct ccagcaataa
    18661 gttgtcgccc tgtgccttcc cgctgacgag ccggatgaac tgctatccgg ctaacccgcc
    18721 ttccgcgcaa tgtcgccgcc agtggattgc tgccgtgcgc cgccagcgac tgggccacca
    18781 gattaccccg cgggcgacga aaacctgccc ataccgcctg actgagttct tgagataatc
    18841 cgccctcatc caccagccac aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa
    18901 aatgttgccc tggtgcatcc atcatccggc gtaaatccag cggcgaggtc cggtagtgcg
    18961 caccagacaa taactgataa acctttaacg gcgtttctgg ctcgcttcgc cataacgttt
    19021 gttcaaatgc ggaaatgacg atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa
    19081 aaaccagtgc ctcactaacc attttttcca gcgggcatcc ctgtgcccag cggatcggct
    19141 gttgcagttc aaaacggtgt aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac
    19201 gtccggtacc ttcgtagccc tgcaccgtag tggttaacaa cgttcgagga aaacgcgata
    19261 ccagttgatg caacaacggc gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg
    19321 cttgctcatc gctggctaac aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa
    19381 attgtgccag tacatccgtt gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa
    19441 tacgagaaat gagttgccct gccaacgccg acttaccgcg cccacgcgca gccgttaccg
    19501 ctgccacgcc cggcggcatg gtcagtagct gctgtaagag ttgctgttgt tctggttgtg
    19561 gtgcgccggt cgcggggtgc cagtcagtac ggggagtaaa atgcgccaac gagaacggct
    19621 ggttttgccg ccagaggata gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga
    19681 caaaatgcgg cgtcgcaata gggtcagggc aatcactcca gcgcagcgag tcggcatcag
    19741 gttggttttc ccactcttcc catacaggga gtaacaaaac cagccagctt cccgctttca
    19801 acgttccgct aagtgctgca aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg
    19861 catgccggaa ctcgcgccca agtaaagttt gtagcgccga gggtgaacag tggttttcag
    19921 catctagctg cggcgaaatc cacagccagt cgccaggtaa ggcatcacgc aacttaagag
    19981 catgatcaaa acaccaaccc tcttccccgc tcaacaccag caagcggcag atcccttcac
    20041 gtttcatttg cgctgttaat gtgtaaagcg cagtcagttc agccatccct gccccggaaa
    20101 ttaaatgctt ttaccaaaag tattgcattg tgccggatcg ccgctgtcga aaccacgttt
    20161 aaaccagctg tagcgttgct gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc
    20221 ctgactttgc tgttgtaaac ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc
    20281 cagatcgcca gtttccagaa cgccttgctg ctgcatacta tgcccccaga caccggcaaa
    20341 acagtcggcc tggagttcca tacgcacaga taagcggttt acttccgcct gcgtcgcgtt
    20401 ttgttgcagt tgacgaactt tcggctcgat gcctaacagt ttctgcacat gatgaccgac
    20461 ttcatgggcg ataacgtacc cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat
    20521 gtcatcatag aaggagagat cgatataaac tgtgccatcc gccgggcaat agaacggccc
    20581 cataatggac tggcccgccc cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt
    20641 cggttgctga taggtcttac ccatcttttc gaactgttgt ccccaggtgt cttccgtggt
    20701 tgccagaatc accgaggtga attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt
    20761 tgattgttgt tgagaaaccg gctgcccggt catcaacccg gttaaatcaa cgccatagta
    20821 gcctgcaacc agcacgacta tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa
    20881 accgggaccg cccattgacg gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact
    20941 ttcacgtcgc ccttgccaac gcatagatac ctcaacaata tattcattat agaaatgatc
    21001 gtaggtggtt cagaggaaga ttaccacagg aaagcgggat gaaatggcgg aggaatgagc
    21061 gttcaatgca tatattccga gagcggttgt cttgccgtgc cagctgcacg gcaagatgat
    21121 gaacagaaaa atcagtccag ctgtacaccc aggcggcggg cgacggcttc ataggcttcg
    21181 atcaggccac cgaggctctg gcggaaacgg tctttgtcca ttttctccag tgtttctttg
    21241 tcccacaggc ggctgccgtc cggggagaac tcatcgccca gtaccacttc acctttgtac
    21301 agaccaaact ccagcttgaa gtcgaccaga atcagaccag catcatcgaa cagttttttc
    21361 agcacgtcgt tcgctttgta ggtcagctct ttcatacgcg ccaggttctc tttgctcacc
    21421 cagccaaagg tttcgcagta agattcgttg accatcggat cgtgcatggc atcgtttttc
    21481 aggaacagat cgaacagcgg cgggttcagc tcaatacctt cttcgattcc aagacgtttc
    21541 accagagagc cagcagcacg gttacgcacg acacactcaa ccggcaccat atccagcttt
    21601 ttcaccagac attcggtatc ggagagcaga cgctccattt gagtcgggat acccgcttca
    21661 gccagtttgc tcataatgaa gtagttgaac ttgttgttca ccataccttt gcgatcaaac
    21721 tgctcaatgc gcgcgccatc ccctgctgac gtatcattgc ggaattcgag caccaacagg
    21781 tccgggtttt ccgtgctgta tacggttttc gctttaccac gatacaactc agcttgcttt
    21841 tgcatcttta tcactcctgg gtgtgaatta acgttttaaa atcttttgct gtctggtgtg
    21901 ccggatgttt tgtcggatgc ggcgtgaacg ccttatccga actacgtccg acaaacaaat
    21961 ttcgtgcgaa ttaccgctaa aatcgcgttt tcctgtcgac gcacacgttt gcgtatcata
    22021 tcagaaaaaa gggccggatg attccagccc tgtattttta cttgctaaac gcagcctgga
    22081 agacagctac cagcgcgtcg ttctgactct gagtcagagt atgacctttc ggatcgatga
    22141 actgtaggct gctgcggtta tctaaatcgc caacctgcag tttatagtca ccggatgcca
    22201 ggcctggatc gctcgcgccc agttcctgcc agtcgctgtc agacagcggc ttataagtta
    22261 cggccatgtt gccctgcgaa cgggtgctgt cggtcacttt catgcccact ttttccagcg
    22321 ccgctggcag acgttgccaa accacattga acggcccgcg tacgaccagc attggtaaac
    22381 cggtgtcatc agctgcactt tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt
    22441 tcgcggcgtc agtggcagat ttatccagac cggcggaaat aacgttcatc atctccgtgc
    22501 tgtaacgctg catggaagcc gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca
    22561 gtttaaccgt aaccgcctgc tgataaccct gcggcttaac agagatttga taacgaccac
    22621 gatactgctc gtcttcgtcc agacggttcc attgtaccca atcggtggtc agtgtctgac
    22681 cagcatcatc acgttgggtg atggtgtagt ttttcgcctg cagcacgcta accacctgcg
    22741 gccacagagt attgccacga ccattttcca ccagcaatga agcggtatcg cccgtgaact
    22801 gggtacgcgc gccagaaacc agtgccaacg gctgggctgg tggacgaatg tccagcgcct
    22861 taccgacagc accactaccg ttggtcaccg ggattgcata atcaccggag gtcaccggca
    22921 aaatcattcc agccggggca tgaagctccg caagcggtgc cgcttccagg taggcttcat
    22981 caccactgac ctgacgctta tagcgtgagt cagaactaca ggcagcgagt aataaaacaa
    23041 gcgaaacacc cgcaaccttt gccaggcgcg acttttgaac agagtaagcc atcaaatctc
    23101 cctaaacttt acagcaaacc ggcatgctta agcgccgctc tgaccgtctc acgaccactg
    23161 tcggtgattg gtgtcattgg caggcgcagc gtatcggtcg ccacaagacc cagttcctta
    23221 catgcccatt tcaccgggat tggattgggt tcgacaaata gtttgttgtg taatggcatc
    23281 agacgctgat taataacgcg tgcctcggca aaatgccctt ctgctgccag tttgcacatc
    23341 tgggccatat cacgcgctgc gacgttagcc gtaacggaaa taactccatg accgcccaat
    23401 tgcatgaagt ccagcgcgct cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc
    23461 agctctttga tctggtttac acgcgttaag ttccctgttg cctctttgat tccgataata
    23521 ttttttactt tcgccagacg gcccaccgtt tccgggagca gatcgcagcc agtacgggac
    23581 ggcacattat acagaatttg cggcaggtca gtatgctcag cgatggcttt gaaatgctga
    23641 tacaaacctt cttgcgacgg acgattgtag taaggggtta ccgtcaggca gccgacgata
    23701 ccactgtcat tgaagcgctg cgtcaggcta atggcttccg cagtagcgtt agcgccggtc
    23761 ccggcaatta ccggaatgcg cccatcagcc agatccagcg tcatcatcac cacatcagca
    23821 tgttcgtcat gatttaaggt agcggactcg ccagtggtgc caacagaaac gatcgccgaa
    23881 gtaccgctgg cgacatgata atcaatcagt tttttcaagc tagcccgaca gacattacct
    23941 ttttcatcca tcggagtaac aatcgcgaca atacttcccg tgaacatggg ccatcctctg
    24001 tgcaaacaag tgtctcaatg gtacgtttgg tatggcatta aaagcaagca gacagaaccg
    24061 ttctgattgt tgtatgcatg atttttttat gctttcctta agaacaactc accccttaaa
    24121 ggaataacca gtttgacact gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc
    24181 gatcgccctg gaattgtgaa caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt
    24241 gaagacagtc gcctggcgat gctgggagaa gagttcacgt ttatcatgct gctttccggt
    24301 tcatggaatg ccatcactct gattgaatca acgttaccgt tgaaaggtgc cgaactggat
    24361 cttttaatcg tgatgaagcg cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc
    24421 tgggttcagg tcgatgttgc agactccccg catttaattg aacgcttcac agcacttttc
    24481 gacgcgcatc atatgaacat tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa
    24541 cgggctgcgc agttgcatat tcagataacc gcccacagcc ccgcatccgc ggacgcagca
    24601 aatattgagc aagcgttcaa agccctatgt acagaactca atgcacaagg cagtattaac
    24661 gtcgtcaatt attcccaaca tgatgaacag gatggagtta agtaatgaat ccactgaaag
    24721 ccggtgatat cgcaccgaaa tttagcttgc cggatcaaga cggagaacaa gttaatttga
    24781 ccgacttcca gggacagcgt gttctggttt atttctaccc gaaagccatg acccccggct
    24841 gtaccgtaca ggcctgcggc ttacgcgata acatggatga gttgaaaaaa gcgggcgttg
    24901 atgtgctggg tatcagcacc gataaacccg aaaaactctc ccgttttgcg gaaaaagagc
    24961 tgcttaactt tacgctcctg tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct
    25021 ggggtgaaaa gtccttcatg ggcaaaacct acgatggtat tcatcgcatc agcttcctga
    25081 ttgacgctga tggcaaaatc gaacatgtct ttgacgattt caaaaccagc aatcaccacg
    25141 acgttgtgct gaactggctg aaagaacacg cctgattact ttgctccatt ccgtgctggc
    25201 tgcgcttgcg gccagcatac ctcacttctc gtgatcaaga tcacattctc gctttcccct
    25261 gcgacacggg tgtcgaatcc attttttgct gaacgttaat gaccatcatt tttgtaccgt
    25321 tcagaatcca gttaatacat aacttattga atagattgag ttaatcagaa tggcatcctt
    25381 tatacaatat gaaatgcaat gtttcatatc attttcaagg agccgacatg aaccgctttg
    25441 tggtggccga accactgtgg tgtacaggat gtaatacctg tctcgctgcc tgttcggacg
    25501 tgcataaaac gcaaggttta cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa
    25561 tcactgcccc tgtcgtgtgt catcactgtg aggaagcccc ttgcctgcag gtctgcccgg
    25621 tcaatgccat ctctcagagg gatgatgcga tccaactcaa cgaaagcctc tgtattggct
    25681 gcaagctttg cgccgtggtc tgcccatttg gcgcaatcag cgcttcagga agccgtccgg
    25741 tgaatgccca tgcgcaatat gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa
    25801 acgtgccgcc ccaacatgcg ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga
    25861 aatgcgacct gtgtgatttc ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc
    25921 aggcgttacg gctgatcacc gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc
    25981 ttgccgcaag ctggtttgcc aatggcgggg agtctcccct ttccctcact caggagcaac
    26041 actaatggat gccctgcaat tattaacctg gtcgctgatc ctctatctgt ttgccagtct
    26101 ggcttcgctg tttttactcg gtctggacag actggctatt aagctttccg gcatcacatc
    26161 gctggtgggc ggcgtgattg gcatcatcag cggaattacg caattacatg ctggtgtaac
    26221 tttagtcgcc cattttgcca ccccttttga ctttgccgat ttaaccctgc gaatggatag
    26281 cctctcggca tttatggtgc tagttatctc cttgctggtg gtggtttgtt cgctctattc
    26341 attgacttat atgcgcgaat acgagggcaa aggcgcggcg gcgatgggct tctttatgaa
    26401 tcttttcatc gcatcgatgg ttgccctgct ggtgatggac aacgcttttt ggttcatcgt
    26461 gctgtttgaa atgatgtcgc tgtcttcctg gtttctggtc attgccaggc aggataaaac
    26521 gtcgatcaac gctggcatgc tctacttttt tatcgcccac gccggatcgg tgctgattat
    26581 gatcgccttc ttgctgatgg ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg
    26641 ctttcacttt ctccggggct ggcgtcggcg gtgtttctgc tggccttttt cggttttggc
    26701 gcgaaagccg ggatgatgcc gttgcacagc tggttgccgc gcgctcaccc tgccgcacca
    26761 tcgcacgctt cggcgttgat gtctggcgta atggtcaaaa tcggtatttt cggcatcctg
    26821 aaagtagcga tggatctgct ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg
    26881 atggcgatcg gcgcaatctc cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat
    26941 atcaaacggc tgctggcctg gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc
    27001 ggtgtggcga tggacggtct gtcactgcac gacccgctgc tcaccgtggt tggactgctc
    27061 ggcgcactgt ttcatctgct caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg
    27121 gacgcgatta tttcgcgttt gcatacccac gacatggaaa aaatgggggc actggcgaaa
    27181 cggatgccgt ggacagccgc agcatgcctg attggttgcc tcgcgatatc agccattcct
    27241 ccgctgaatg gttttatcag cgaatggtac acctggcagt cgctgttctc actaagtcgt
    27301 gtggaagccg tagcgctaca acttgcgggt cctattgcta tggtaatgct ggcagtcact
    27361 ggtgggctgg cagtaatgtg cttcgtcaaa atgtacggta ttactttctg tggtgcgccg
    27421 cgcagtacac acgctgaaga ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta
    27481 ctgctcgcgg cactctgcgt attaattgcg cttagtgcca gttggctggc accgaagata
    27541 atgcatattg cccatgcgtt taccaatacc cctcccgtca ctgtcgccag cggaatagca
    27601 cttgtacccg gcacgtttca tacacgggtc actccttcat tactgttgct gttactactg
    27661 gcgatgcctt tgctgccagg cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt
    27721 cgtcgcacag gagatgcctg ggcatgcggc tacggctggg aaaacgcgat ggctccgtca
    27781 ggcaatggcg tgatgcagcc gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa
    27841 cagctcgacc ctacgctgag gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag
    27901 agcacagaac ccttctggga tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg
    27961 ctggtcaaag aaatacagca tctgcaaagc ggcgactttc gtctctattg cctgtatgtg
    28021 gtcgccgcac tggttgtgct gctaatcgct attgccgtct aaggaaatca ccatgagaca
    28081 aactctttgc gacggatatc tggtcatttt tgcgttagca caggccgtga ttctgctgat
    28141 gctaacccca ctttttacgg gtatttcccg gcagatacgc gcgcgtatgc actcccgccg
    28201 cgggccgggg atctggcagg attatcgcga tatccacaaa ctgtttaaac gccaggaagt
    28261 tgcgccgaca tcttcaggtc tgatgttccg cctgatgccg tgggtattaa tcagcagcat
    28321 gctggtgctg gcgatggcct taccactatt tattaccgtt tccccttttg cgggcggcgg
    28381 cgatctgatc acccttatct atcttcttgc cctgtttcgt tttttctttg ctctttccgg
    28441 gctggatacc ggaagtccgt ttgcgggagt cggtgccagt cgcgagttga cgctcggcat
    28501 tctggtcgaa ccaatgctta ttctctcact gctggtattg gcgctgatag caggttccac
    28561 gcatatcgag atgatcagca atacgctggc tatgggctgg aactcgccgc taaccaccgt
    28621 actggcgtta ctggcctgtg gttttgcctg cttcattgag atgggaaaaa ttccctttga
    28681 tgttgctgaa gcagaacagg aattacagga aggcccgctg accgaatatt ccggtgccgg
    28741 gctggcgcta gcgaaatggg ggctggggct gaaacaggtc gtgatggcag cactgtttgt
    28801 ggccctgttt ctgccctttg ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc
    28861 acttgtcgtt acgctgctca aggttttgct gatttttgta ctggcctcaa tcgcagaaaa
    28921 cacgctggca cgcgggcgtt ttttactcat tcaccatgtg acctggcttg gcttcagcct
    28981 tgctgcgctt gcatgggtct tttggttaac cggtctgtaa ggagcactga cggaatatgg
    29041 aaaatcttgc tctgacgacg ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt
    29101 cgccacaacg tcgggccgcc gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca
    29161 tgttgtcgct gatctccgcg ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg
    29221 tcaacgtggg ggatgtggca ttgtttggcc tggtcattga tcgcgtgagt acgctgattc
    29281 tgtttgtggt ggtgtttctc ggtttgctgg tcacgatcta ctccacgggt tatctgacgg
    29341 ataaaaatcg cgaacacccg cataacggca cgaatcgtta ttacgcattt ttgctggtgt
    29401 ttatcggcgc gatggcggga ctggtagtct cctcgacgct gctcggtcag ttgttgtttt
    29461 ttgaaattac gggcggctgc tcctgggcgt tgatcagtta ttaccagagc gataaagcgc
    29521 agcgttcagc actaaaagcg ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg
    29581 ccgccgccac gctgtttttg cagaccggaa cgtttgtgct tagcgcgatg agcgagttac
    29641 atggcgacgc acgttatctg gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg
    29701 cccagctacc gatgcaagcg tggctaccgg acgcaatgga agcgccaaca ccgatcagcg
    29761 cctatctcca cgccgcatcg atggtgaaag tgggcgttta catttttgcc cgcgccatta
    29821 tcgacggcgg caatatcccg catgtgattg gcggcgttgg catggtcatg acactggtca
    29881 ccattcttta tggctttctg atgtatttgc cacagcagga tatgaagcgg ttgctggcct
    29941 ggtcgaccat cactcaactt ggctggatgt tcttcggctt gtcgctctcc atcttcggct
    30001 cgcggctggc gctggagggt agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc
    30061 tgtttttcct tgtagcaggt gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc
    30121 tgcgtggcgt attgcacacc ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg
    30181 caattaccgg tgtgccgccg ttcaacggct tcttcagtaa attcccgctg tttgccgccg
    30241 gttttgcgtt gtcagtggag tactggatcc tgctgcccgc catgactctg ctgatgattg
    30301 aatcggtcgc cagtttcgcc tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac
    30361 cgagcgaggc cgtcgccgat gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg
    30421 tactgattgt gatgtcgctg atttccagcg taatcgccgc gacctggttg cagtaaggag
    30481 atgatgaatg accggttcta tgatcgtaaa taatctggcg ggactgatga tgctgacatc
    30541 gctgtttgtg attagcgtca aaagctatcg cctgtcatgc ggattttacg cctgccagtc
    30601 gctggtgctg gtgtctattt tcgccactct ctcgtgcctg ttcgccgcag agcaactgct
    30661 gatctggtcc gccagcgcct ttatcaccaa agtgctgctg gtaccgttaa tcatgactta
    30721 cgctgcacga aatattcccc agaacatccc ggaaaaagcg ttattcggtc cggcaatgat
    30781 ggcactgctc gcggcgttaa ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct
    30841 accgatggct accgggctga aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg
    30901 cctgctgtgc attgtcagcc aacgcaatat cctgcggcaa atttttggtt actgcctgat
    30961 ggaaaacggc tcccatctgg tgctggcgct tcttgcctgg cgagcaccgg aactggtgga
    31021 aataggtatc gctaccgacg ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa
    31081 aatatggcgt acccacggca cgctggacgt gaacaacttg accgcgctga agggataatg
    31141 agatgagtta ttctgtgatg ttcgctttac tcctgctcac gccgctgctt ttttcgctgc
    31201 tctgttttgc ctgccggaaa cgggggctct ctgcgacttg tacggtgact gtattacata
    31261 gcttagggat cacactgctg ctgattctgg cactctgggt ggtccaaact gccgctgatg
    31321 caggagaaat attcgctgcg ggactgtggc ttcatattga tggtctgggc ggtttgttcc
    31381 tcgccattct tggtgtgatt ggctttctca ccggtgttta ctcgattggc tacatgcgtc
    31441 atgaagtggc acacggcgag ctttcacccg ttacactgtg cgattactac ggtttcttcc
    31501 atctgttttt gttcaccatg ctgctggttg ttaccagcaa taacctgatt gtgatgtggg
    31561 cggcgatcga agccaccacc ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt
    31621 catcgctgga atcagcatgg aagtacatca ttatttgtac tgttggtgtc gcttttggtc
    31681 tgttcggtac cgtgctggta tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg
    31741 cgatattctg gagcgaggtt cttaagcaat cgtccttgct tgacccaaca ttaatgctgt
    31801 tggcctttgt gtttgtgcta attggctttg gtaccaaaac cgggctattt cccatgcacg
    31861 cctggctgcc ggatgctcac agtgaagcgc cgagtccggt cagcgccctg ctctccgccg
    31921 tattgctgaa ctgcgcgctg ttggtgctga ttcgctatta catcattatt tgccaggcca
    31981 tcggcagcga tttccccaac cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg
    32041 cggcattttt cattctggta cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg
    32101 agaacatggg gctggtcgcg gtggcgctag gcattggcgg gccgctggga atttttgccg
    32161 cgctgctgca caccttaaac cacagtctgg caaaaacgct gctgttctgc ggttccggca
    32221 atgtactgct caagtacggc acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca
    32281 tgccatttac cgccgtgctg tttggcggcg gtgcgctggc gctggcgctg gcagggatgc
    32341 cgcccttcaa catttttctt agcgaattta tgaccgttac cgcaggactg gcacgtaatc
    32401 acctgctgat tatcgtcctg ctgttattgc tgttaacgct ggtgctggcg ggcctggtac
    32461 ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg
    32521 gctggttgac cacctcgcca atggtgattc tgctggtcat gatgctggcg atgggaacgc
    32581 atattccaca acctgtcatc aggatcctgg cgggcgcttc cactatagtc ctctcaggga
    32641 cgcacgacct gcctgcacaa cgtagcacct ggcatgattt tttgccttca ggcaccgcat
    32701 ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa atcgtggcga agcgattctc
    32761 gccgccctga aaacgcagtt ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa
    32821 caggtcacca ttacggtgaa aatcaatctg ctgcctgacg ttgtacatta tctttattat
    32881 caacatgatg gctggcttcc agtcctgttt ggcaacgacg agcggacact taacggtcat
    32941 tacgcggttt attatgccct ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag
    33001 gcactggtcg atgccgacag tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg
    33061 gtctggggcg agcgagaaat tcgcgatatg tacgggctga ttccggttgg cctgccggat
    33121 cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata tgcatccgct gcgcaaagat
    33181 gcgatggatt atcgactgcg ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc
    33241 aatgagggca acagcgatgc gcgggtgatc cctgtcggcc cgctgcatat cacctcctat
    33301 gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc
    33361 ctgttttatg tccatcgcgg catggagaaa ctggcagaaa cgcggatggg ctacaacgaa
    33421 gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat
    33481 accaactcgg ttaaaaatgc actggggatt gaggtgccgc aacgagcaca tactattcgc
    33541 tcgattctgc tggaagtcga gcggctacac agtcatttgc tcaaccttgg cctctcctgc
    33601 cattttgttg gttttgatac cggctttatg caatttttcc gcgtgcggga aaagtcgatg
    33661 acgatggcgg aattgctgac cgggtcgcgt aaaacctacg gtctgaatct gattggtggt
    33721 gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg
    33781 cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc
    33841 actcagggca ttggcattct cgaccgacaa atcgcccgtg attatagccc tgtagggccg
    33901 ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga tcacccctac gccgactacg
    33961 gcaatattcc aaaaacactg tttaccttta ccggcggcga tgttttctcc cgcgtgatgg
    34021 tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc gacaacatgc
    34081 cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc gcgctgggct
    34141 ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat aaccaaaaat
    34201 tgttccgctg gcgctgtcgt gccgccacct acgccaactg gccggtgttg cgttacatgc
    34261 tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat ccctgctact
    34321 cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt
    34381 ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag taaggacaga
    34441 agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg aaatatccct
    34501 tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg cccagccaat
    34561 gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa tgcgctgact atccagaccg
    34621 acgaccagca aaattcgcgc acctggcagc tctatctggg gcgttgtatt tactgcggac
    34681 gttgtgaaga agtgtgcccg accagagcca tccagcttac caataacttt gaactgaccg
    34741 tcacccataa agccgatctc tatacccacg cgacgttcca tctacaacgt tgcagccgtt
    34801 gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc tgctgaattg ttggcacagc
    34861 aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt
    34921 gcaaacaacg cgcgacgctg atcaacgacg atacagatgt accgctggtg gctaaggagc
    34981 agctatgagt ccagtgctta cgcaatatgt cagccagccc atcacgctgg acgagcaaac
    35041 gcaaaagatg aagcggcatt tgctacagga tatccgtcgc tcggcttacg tttatcgcat
    35101 cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt gctgccatca cccccgtatt
    35161 cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt
    35221 atttactggc acagtcaccc gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc
    35281 ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca
    35341 cgatctctac agcgtctggg gcggcagcga caccattgtc cccattgatg tttggatccc
    35401 cggctgcccg ccaacaccgg ccgccaccat tcacggtttc gccgtggcgc tcggtttgct
    35461 gcaacagaag attcacgcgg tggattatcg cgatcccacc ggggtgacta tgcaaccgtt
    35521 gtggccgcag atcccgccat cacagcgtat cgccattgag cgagaagcgc ggcggctggc
    35581 gggctatcgt caggggcgag aaatttgcga tcggctcctg cgccatttaa gcgacgatcc
    35641 tacaggaaat cgggttaaca cctggttgcg cgatgccgac gatccacgtc tcaatagtat
    35701 cgttcagcaa ctctttcgcg tactccgggg gttacatgac tgaagagtgc ggggaaattg
    35761 ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga cgagatgccg gaacacagct
    35821 ctcaggtaat gtattactcg ctggctatcg gtcatcacgt tggcgtgatt gattgtctga
    35881 atgtcgcctt ccgctgccca ctgacggaat acgaagattg gcttgcactg gtcgaagagg
    35941 agcaagcccg acgtaagatg ctgggggtga tgacttttgg tgagattgtt attgacgcca
    36001 gccacaccgc cctgttgacc cgggcattcg cgccactggc agatgacgcg acgtctgtgt
    36061 ggcaagcgcg tagcattcaa ttcattcatc tgttggatga aattgtgctg gaaccggcta
    36121 tctatctgat ggccagaaaa attgcgtgag aaggatttct cattaataag gactgttgat
    36181 ggctatgtca gacgaagcga tgtttgcccc gccgcaagga ataacaattg aagcggtaaa
    36241 cggaatgctc gcggagcggt tagcgcagaa acacggcaag gcgtctttat tacgcgcctt
    36301 catcccgctg ccgccgccgt tcagcccggt acaacttatt gaactgcatg ttctcaaaag
    36361 caacttctat taccgctacc atgatgatgg cagcgatgtg acggcaacaa cagagtatca
    36421 gggcgagatg gtcgattatt cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga
    36481 gctacgcttt attcgtaccc acggcagccg ttttactccc caggattgca cactgtttaa
    36541 ctggcaggca cgcattatca ccccggttct gcaatcatgg ctcaatgatg aagcacagca
    36601 ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat cgggtactgg tggatatcac
    36661 taatgcagtg ctgtcacatc ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat
    36721 ccatcatttt ttcggtctgt cttcagtcag tatggtactg ggcgatcatc gaaagaacga
    36781 gaagttcagc ctgtggtgca gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa
    36841 tatgcctggc gacagtgtat tgctgacaca aacgctacaa acccgacaac cgaccttgac
    36901 gcaccgtgca gatgatctgt ttctctggca acgcgacccg ttattactct tacttgcatc
    36961 taacggctgc gaatctgcgc tccttatacc gcttaccttt ggcaaccata caccgggtgc
    37021 attgttgctg gcgcatacct cttccactct ctttagtgag gaaaactgcc agctactaca
    37081 acacatagcc gatcgcatcg ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac
    37141 cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa
    37201 tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg gaagacctgc tccagcaggt
    37261 agatattgtg gcgaagagcg acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa
    37321 agaggtgatc gccagagcga tccatcaact tagcccgcga cacgacaagc cgctggtcaa
    37381 aatcaactgc gctgccatcc ccgccagtct tctggaaagt gagttattcg gtcatgacaa
    37441 aggggcgttt actggtgcga ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg
    37501 cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa cttcagccta aactgctgcg
    37561 cgtattgcag gaacgggaga ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt
    37621 cagagtcatt gccgccacca accgtgattt gtggcaaatg gttgaagatc gccagtttcg
    37681 cagcgatctc ttttatcgcc tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg
    37741 tccggaagat atccctcttt tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa
    37801 tcgttcaatt gacgccatcc cgaccgaggc gctacgccag ttgatgtcgt gggactggcc
    37861 gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa
    37921 cagtctgaat ttacatctta atgtccgaca aagccgttta ctgccgacgc taaatgaaga
    37981 ttcagcgctt cgcagttcaa tggcgcagtt gctgcacccg acgacgccag agaatgacga
    38041 agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc
    38101 ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg
    38161 tctgggaatc tcggttcgcg aggtgttgta atctgctttt gcaggagtat gcatgagaaa
    38221 caaactctct ttcgacttgc agttgagcgc cagaaaagcg gcaatcgctg aacggattgc
    38281 cgcccataaa attgcccgca gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt
    38341 gtttatggcg atcggattta ctttttacct ttccgttatc gccgatgccc cgtcttcata
    38401 ggcattaacc catctggtgg gcggcctttg ctttacactt ggctttattt tgctggcggt
    38461 ttgcggcacc agcctgttca cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt
    38521 tattagttgg cgaacttggc tgattaacgc acttctggtg gcctgcggta atctggcagg
    38581 tattgcctgt ttcagtttgt taatctggtt ttccgggctg gtgatgagtg aaaacgcgat
    38641 gtggggagtc gcgattttac actgcgccga gggcaaaatg catcatacat ttactgaatc
    38701 tgtcagcctc ggcattatgt gcaatctgat ggtttgcctg gcgctgtgga tgagttattg
    38761 cggacgttcg ttatgcgaca aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc
    38821 cagtggcttt gagcactgta tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg
    38881 ccatttcgcc cctacctcct tctggcaact ggcgcacagt agcgcagaca attttccggc
    38941 actgacggtc agccatttta ttaccgccaa tctgctcccg gtgatgctgg gtaatattat
    39001 cggcggtgca gtgctggtga gtatttgtta tcgggctatt tatttacgtc aggaatcctg
    39061 aaaaatcagc ccggcgaaac agttcgtcgg gctgaagatt attcttgcgc gatttgccca
    39121 tcgggccagg cgtggatcac ggctttgatc agcgtcgcca atggaatggc gaagaatacg
    39181 ccccagaatc cccacaaacc accgaagatc accaccgata aaataatcac cagcggatgc
    39241 aggttaaccg cttcggagaa caacaccggt actagcaggt tgccgtccag cgcctgaata
    39301 atcagataca ccgcgaagca gctccagaat tcagtacctg cgccaaactg gaatagcgcc
    39361 acgccaacca ccggaatggt caccacaaat gcgccaatgt acggaataag aaccgagaag
    39421 ccgaccagca ctgccagcag cagcgaatag ttcagcccga agagcaagaa ccccagccag
    39481 gtggcgatcc ccaccacgat catctccagc actttgccgc ggatatagtt ggtgatttgt
    39541 tgattcatct ccttccacac ctgtcctgcc agcccacggt tacgcggcag cacccggcga
    39601 acggcgttca gcatctgctc tttgtctttc agcaggaaga agaccatcaa tggcaccagc
    39661 accagataga cggctatggt cagcaaaccg accagcgagg cgagggaaat tttcaccacc
    39721 gaatcgccca tggtcagcat ccgactgcgc atattttcgg ccattgcatc aataatgccc
    39781 gcgtccatta acaccggata gcggcgcggc aacgtggcgg caaagtcaga aagcttattg
    39841 agcatccccg gcatatcgcg gattaagtag atgccctgtt gccaggcgat aggcagtacc
    39901 acgaacgcca tcagtagcaa tataccgaca aaaaccacca atacaatcga cgtcgcccag
    39961 cggcgggagc agccaataga ttgcaggcgc acggttggcc attccagcaa ataagccagc
    40021 acaatagcca ccagcaacgg agcaagcagg ccactaaaga aaaagataat gccaaatccg
    40081 gcaactaaaa taaccagcaa ggcaatcgct tccgggtcgc taaaacggcg gcgataccat
    40141 tgcatcaaca tttcgagcat aaagaccttc cctgaacctc aagagcggga ttgcgatccg
    40201 caattgtatc gaaatgtcac aaaaaagact tcgcttttta tgacggattc aggaaactaa
    40261 aaagtcattt gagtgggcta atcttcgccg ttacactcaa aggcggcgag gtgggaacga
    40321 tatttcacag tatcggtcaa atgactatct tcagaaatac aggatagagg ttatgttcag
    40381 gcagttgaaa aaaaacctgg ttgcaaccct cattgctgct atgaccattg gtcaggtagc
    40441 cccggcgttt gccgacagcg cagacacctt gccggatatg ggaacctccg caggaagcac
    40501 gctttccatt ggtcaggaaa tgcagatggg cgactattat gtccgccagc tacgcggcag
    40561 cgcgccgtta attaatgacc cgctgttaac gcaatatatt aattcgctgg ggatgcgtct
    40621 ggtttcgcat gccaattcgg ttaagacacc gtttcatttt tttctgatca acaacgacga
    40681 aattaacgcc tttgctttct ttggcggcaa cgtggtgctg cactctgccc tgttccgtta
    40741 ttccgataac gaaagtcaac tggcttcagt tatggcgcac gaaatctccc acgtcaccca
    40801 acgtcacctg gcgcgagcga tggaagatca acaacgcagc gcgccgctga cctgggtcgg
    40861 cgcgttaggt tctattttac tggcgatggc cagtccgcag gcggggatgg cggcgctgac
    40921 cggtacactg gcgggaacgc gccaggggat gatcagtttc acccagcaaa atgaacagga
    40981 agcggaccgc attggtattc aggtgctgca acgctcggga ttcgatccac aggcgatgcc
    41041 gaccttcctg gaaaaattgc tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt
    41101 attgactcac ccgttgccgg aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat
    41161 gcgcccgatg gtggtgcagt cgtcggaaga tttctatctg gcgaaagcgc gcacactggg
    41221 gatgtataat tccggacgta accagctcac cagtgatttg ctggatgaat gggcgaaagg
    41281 aaacgttcgt cagcaacgag cggcgcaata tggtcgtgct ttacaggcga tggaagccaa
    41341 taaatacgac gaggcgcgta aaacgctgca accgttactg gcggcagaac ctggcaacgc
    41401 atggtatctc gatctggcta ccgatatcga tcttgggcaa aacaaagcca atgaggcaat
    41461 caatcgcctg aaaaatgccc gcgatttacg caccaatccg gtgttgcagc tcaacctggc
    41521 gaacgcttat ctgcaaggcg gtcaaccaca agaagcggcc aatattctga atcgctacac
    41581 ctttaataat aaagatgaca gcaacggctg ggatttgctg gcacaggcgg aagccgcgct
    41641 aaataaccgc gatcaggagc tggctgcgcg agcagaaggt tatgcgctcg ccggacgact
    41701 cgatcaggcc atttcgctgt tgagtagcgc cagttcgcag gtaaaattag gcagcctgca
    41761 acaagcgcgt tacgatgcgc gcatcgacca gttgcgccag ctgcaggaac gctttaagcc
    41821 ttataccaag atgtaattcg cattaaagga agaacaatga ccaaacaggt aaaaatctac
    41881 cataacccac gctgttcaaa gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg
    41941 gagccagaag tggtgctcta ccttgagaca cccgccgatg cggcaacgct gcgcgatttg
    42001 ctgaaaatgc tggggatgaa tagcgcccgt gaattgatgc gccagaaaga ggatctttat
    42061 aaagaactaa acctggctga cagttcgctt agcgaagagg cgctgattca ggcaatggtg
    42121 gataatccaa agctgatgga gcgcccgatt atggtcgcga atggcaaagc gcggattggt
    42181 cgtccaccgg agcaggtact ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag
    42241 cacattgccg gatgcgacgc ttgccacgtc ttatccggcc tacgaatact atggattaac
    42301 ttcgcgtagg tcaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag
    42361 aatttctttc acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa
    42421 ctgatccaac gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg
    42481 ccccacatct tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa
    42541 cttatcttca tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc
    42601 gagatccggt aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg
    42661 tgttttgccc gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc
    42721 atcgcctgca atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc
    42781 gagcacttcc ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc
    42841 acgctgcgac aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg
    42901 tgcccagaga tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcggccag
    42961 taaagaggag ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag
    43021 tggcaaagag agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg
    43081 cgagaagttt accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg
    43141 tacattattt gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca
    43201 gcacactgat cagtttgaaa atcaaactta agccgatacc gacgatggtc gccagcgcca
    43261 taccttttaa ctccgccgcg ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa
    43321 tcacggaagt caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa
    43381 cacgaatacc ggaagcacca atgacaccat aaagcagcag cgaaacgccg cccatcaccg
    43441 gcaatgggat catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga
    43501 aaatcgccgc cccgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa
    43561 tgttttctcc gtaagtggta tttggcgtag agccaaagaa gccggagatc acggtcgaca
    43621 ggccgttagc aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga
    43681 tattagccgt tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca
    43741 gaatagtcag aatggcaaac cactcgaagc gcggcgtata gagggttggc agtgcaaacc
    43801 agtgagcatt aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc
    43861 ccaccagcac gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac
    43921 ctaaaaccgt gaccgccagg gtggtaatag agatgatgat ggttttggag tttggcgttt
    43981 gcccttcagc cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga
    44041 tgacggcaac gattgcgccc attgccgcag gtggaaacag cacgtccaac cagccggtcc
    44101 ccgctttctt cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc
    44161 ccagcgcgac ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc
    44221 tggaaccaag ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa
    44281 taccgttaaa taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg
    44341 ttgcaccaaa catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg
    44401 gtctttcact caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta
    44461 taggcgcttt actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt
    44521 tatttcgtac caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc
    44581 tcgttcagtc cctggtcaat cgatgcagta tacagttcga cgtccgggtg cgctttttcc
    44641 agcgcagcga taccttctgg cgcagcgacc agcaccagaa ctttgatgct gctgcagccc
    44701 gcttttttca gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca
    44761 acgatcagcg ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc
    44821 tccagcgttt cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacgttt
    44881 tccagcacac cgtccatcat accaagaccc gcacgcagaa ttggcacaac ggtaattttc
    44941 ttacctttga tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc
    45001 gtttcgaggt cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg
    45061 cgaaagcgct tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt
    45121 gggtgtttga cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa
    45181 atcgacggat tatacctcct ttcttcaagg cggcaatatt attttcgttg actttagtca
    45241 aaatgataac agtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat
    45301 tccatccgaa aactaacctt tgccctggca caagtcttct ttcgccgcgc gcccggggaa
    45361 aagacgtgca aaaaggttgt gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat
    45421 tgcgccgaat tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata
    45481 aaacctctct tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg
    45541 gaagaatcaa aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg
    45601 gcttcggtgc gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca
    45661 ctgacggcgt aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg
    45721 gtattgatct ggtcgccatg tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt
    45781 ttttcctcga ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca
    45841 gcggcatcgc ggaaggttgt ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag
    45901 aaatgccggg gatgtatcac ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg
    45961 tagaaaaatc agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac
    46021 tcggttccag cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca
    46081 gcggttgtga tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg
    46141 caccgacccg catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg
    46201 ccattgcgca cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata
    46261 atacccaggc agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc
    46321 aaacggcagg taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga
    46381 tgattatcgc cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg
    46441 gtgaaaacgc gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta
    46501 tcgaataatg aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat
    46561 tgacgcctgt aaaaccaaca aaattaaagg caccgtacgg gcagttttca gcaataaggc
    46621 cgacgcgttc ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc
    46681 cagcgcgttt gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta
    46741 cgcacccgat gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc
    46801 ccactatgcc gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt
    46861 acacacccat cgtcaggcgc tggaaaatgg cgatgaagag cacggtacat cggtgcattt
    46921 cgtcaccgat gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc
    46981 tggtgatacg gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc
    47041 actggtgatt agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct
    47101 ggatggtcaa cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc cccgtagtta
    47161 aagcgccagc tctgccgctg gcgtttttca attcacctgt aaatcgcaag ctccagcagt
    47221 ttttttcccc cttttctggc atagttggac atctgccaat attgctcgcc ataatatcca
    47281 ggcagtgtcc cgtgaataaa acggagtaaa agtggtaatg ggtcaggaaa agctatacat
    47341 cgaaaaagag ctcagttggt tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa
    47401 atctaacccg ctgattgaaa ggatgcgttt cctggggatc tattccaata accttgatga
    47461 gttctataaa gtccgcttcg ctgaactgaa gcgacgcatc attattagcg aagaacaagg
    47521 ctccaacgct cattcccgcc atttactggg caaaattcag tcccgggtgc tgaaagccga
    47581 tcaggaattc gacggcctct acaacgagct attgctggag atggcgcgca accagatctt
    47641 cctgattaat gaacgccagc tctccgtcaa tcaacaaaac tggctgcgtc attattttaa
    47701 gcagtatctg cgtcagcaca ttacgccgat tttaatcaat cctgacactg acttagtgca
    47761 gttcctgaaa gatgattaca cctatctggc tgtggaaatt atccgtagcg atagcatccg
    47821 ttacgcgctg ctggagatcc catcagataa agtgccgcgc tttgtgaatt taccgccaga
    47881 agcgccgcgt cgacgcaagc cgatgattct tctggataac attctgcgtt actgccttga
    47941 tgatattttc aaaggcttct ttgattatga cgcgctgaat gcctattcaa tgaagatgac
    48001 ccgcgatgcc gaatacgatt tagtgcatga gatggaagcc agcctgatgg agttgatgtc
    48061 ttccagtctc aagcagcgtt taactgctga gccggtgcgt tttgtttatc agcgcgatat
    48121 gcccaatgcg ctggttgaag tgttacgcga aaaactgact atttcccgct acgactccat
    48181 cgtccccggc ggtcgttatc ataattttaa agactttatt aatttcccca atgtcggcaa
    48241 agccaatctg gtgaacaaac cactgccgcg tttacgccat atttggtttg ataaagccca
    48301 gttccgcaat ggttttgatg ccattcgcga acgcgatgtg ttgctctatt atccttatca
    48361 cacctttgag catgtgctgg aactgctgcg tcaggcttcg ttcgacccga gcgtactggc
    48421 gattaaaatt aacatttacc gcgtggcgaa agattcacgc atcatcgact cgatgatcca
    48481 cgccgcacat aacggtaaga aagtcactgt ggtggttgag ttacaggcgc gtttcgacga
    48541 agaagccaac attcactggg cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc
    48601 tgcgccgggg ctgaaaattc acgccaaact gttcctgatt tcacgtaaag aaaacggtga
    48661 agtggtccgt tacgcacaca tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta
    48721 tactgactat tcgttgctga ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt
    48781 taactttatt gaaaacccat accgcccggt gacatttgat tatttaatgg tgtcaccgca
    48841 aaactctcgc cgtctgttat atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg
    48901 gctgcccagt ggtatcaccc tgaagctaaa taaccttgtc gataaaggcc tggttgatcg
    48961 tctgtatgcg gcctccagct ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc
    49021 gctgatcccc aatctggaag gcattagcga caacattcgt gccatcagta ttgttgaccg
    49081 ttaccttgaa catgaccggg tttatatttt tgaaaatggc ggcgataaaa aggtctacct
    49141 ttcttccgcc gactggatga cgcgcaatat tgattatcgt attgaagtgg cgacaccgct
    49201 gctcgatccg cgcctgaagc agcgggtgct ggacatcatc gacatattgt tcagcgatac
    49261 ggtcaaagca cgttatatcg ataaagaact cagtaatcgc tacgttcccc gcggcaatcg
    49321 ccgcaaagta cgggcgcagt tggcgattta cgactacatc aaatcactcg aacaacctga
    49381 ataaccctat gccaatacac gataaatccc ctcgtccgca ggagtttgct gcggtcgatc
    49441 ttggttcaaa cagttttcac atggtcatag cccgtgtggt agatggtgcc atgcagatta
    49501 ttggccgcct gaaacagcgg gtgcatctgg cggacggcct ggggccagat aatatgttga
    49561 gtgaagaggc aatgacgcgc ggtttgaact gtctgtcgct gtttgccgaa cggctacaag
    49621 ggttttctcc tgccagcgtc tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg
    49681 ccactgactt tctgaaacgc gcggaaaagg tcattcccta cccgattgaa attatttccg
    49741 gtaatgaaga agcccgtctg atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc
    49801 gcaaactggt tattgatatt ggcggcggat ctacggaact ggtgattggt gaaaatttcg
    49861 aacctattct cgttgaaagc cgccggatgg gttgtgtcag ctttgcccag ctttattttc
    49921 ctggcggggt catcaataaa gagaattttc agcgcgctcg catggcggca gcacaaaaac
    49981 tggaaacttt aacctggcaa ttccgtattc agggctggaa cgttgcaatg ggcgcttccg
    50041 gtaccataaa agccgcccat gaagtgttaa tggaaatggg cgagaaagac gggataatta
    50101 ccccggaacg tctggaaaaa ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc
    50161 tgagtttacc gggtctttcc gaagagcgga aaacagtctt cgttcctgga ctggcgattt
    50221 tatgtggtgt gtttgatgct ttagccatcc gtgaactgcg cctttctgac ggggcgttac
    50281 gcgaaggcgt actgtatgaa atggaaggac gtttccgtca tcaggatgtg cgtagtcgca
    50341 ccgccagcag cctcgccaac cagtatcaca tagacagcga acaggcccga cgagtgctgg
    50401 ataccactat gcaaatgtac gaacagtggc gggaacagca accgaagctg gcgcatccgc
    50461 aactggaggc gctactgcga tgggccgcca tgctgcatga ggtcgggttg aatatcaacc
    50521 acagcggttt gcatcgccac tccgcttata ttctgcaaaa cagtgacttg ccgggtttta
    50581 atcaggaaca gcagctgatg atggcgacac tggtgcgcta tcaccgtaaa gcgattaagc
    50641 tcgacgatct gccgcgcttt accttgttta agaagaaaca gttcctgcca ctgatacagc
    50701 tattgcgcct tggcgtatta ctaaacaatc aacgtcaggc aaccaccaca ccgccaacat
    50761 tgacactgat tactgatgac agtcactgga cactgcgttt cccgcatgac tggtttagtc
    50821 agaatgcgct ggtactgctt gatctggaaa aggagcaaga atactgggaa ggcgtggctg
    50881 gctggcggtt gaaaattgaa gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg
    50941 gccgacgaga aatgtcggcc cgcattattc aggcactttc gcgaatgggt tcgatttcat
    51001 tcagcgtatc aattaacggt tgcggcttac caataagata accctgcata taatcgatcc
    51061 ccaaagagag caccgcctcg cggatctctt cgttttcaac gtactctgcc actacccgca
    51121 ttttcttcat tcgcgccaga tggcaaatcg acgccacaat ctgataatcc agactatttg
    51181 acacaatatt gcggataaag ctgccgtcaa ttttaagcag atcggcattc acatttttaa
    51241 gccgtgcata gctggcgtag ccggtgccga aatcatcaat cgcaatctgg cagcctaatt
    51301 cctgaagatg ctgcaaggtg atttgcgcct gcttaacatt ggtcagagca ttactttcgg
    51361 tgacttcaaa aataagttgc cacgcttcaa tctgatattt agccagcaac tgactgactt
    51421 caacaggaaa acgagcctga cataccgagg ttggagacag attaatagca aaacggtgag
    51481 cgggcatctt cgctctgttt tcagccataa attgcagcgt atgctcaatg acccacatgt
    51541 cgatactcga cgataaacca aattcgtgcg cgaccggcaa gaagctaccg gggccgatca
    51601 gttcatcatt ctcacctttc atgcgcagaa gaatttcatg gtaaacatca ccacgcatac
    51661 cggtaatcgg ctgggccatc aggaaaaaat ggttgtgttc cagcgcctgc tgtagtcgat
    51721 tcatcatcgc gactttatct ttcaattcgc gttgcaaata cattgccccg caacgctgca
    51781 tattttccgg ggcgttggtc acgatggaaa gttcggcgac cgtatttagc tctcccagca
    51841 gcaggtagat atgattcact ggcgagcgca catagcagta actgacgcca atctgcggtt
    51901 gcatcggcat cccatcccaa aagaaacgaa attgcttgag atgactatcc agtgcggtaa
    51961 tgcgctcctg gtgcgattct gtattcagtc gcagcgcgag atcgttaccc gaaagctgat
    52021 aaacatcttc acccggttcc agcaagggtg acagccagtg agaaagtttt tgctt
//
LOCUS       DADLCQ010000010        51658 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000010 DADLCQ010000000
VERSION     DADLCQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.010"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="JQG95_000543"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="JQG95_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY5887216.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="JQG95_000544"
     CDS             complement(1150..1236)
                     /locus_tag="JQG95_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY5887217.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JQG95_000545"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JQG95_000545"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY5887218.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JQG95_000546"
     CDS             complement(1742..3466)
                     /gene="ilvI"
                     /locus_tag="JQG95_000546"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY5887219.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JQG95_000547"
     CDS             complement(3784..4728)
                     /gene="leuO"
                     /locus_tag="JQG95_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY5887220.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="JQG95_000548"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="JQG95_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY5887221.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="JQG95_000549"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="JQG95_000549"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY5887222.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="JQG95_000550"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="JQG95_000550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY5887223.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="JQG95_000551"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="JQG95_000551"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY5887224.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="JQG95_000552"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="JQG95_000552"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY5887225.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JQG95_000553"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="JQG95_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY5887226.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JQG95_000554"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="JQG95_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY5887227.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /locus_tag="JQG95_000555"
     CDS             11797..13452
                     /locus_tag="JQG95_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY5887228.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="JQG95_000556"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="JQG95_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY5887229.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="JQG95_000557"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="JQG95_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY5887230.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="JQG95_000558"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="JQG95_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY5887231.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="JQG95_000559"
     CDS             complement(16981..17745)
                     /locus_tag="JQG95_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5887232.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="JQG95_000560"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="JQG95_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY5887233.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQR
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="JQG95_000561"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="JQG95_000561"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY5887234.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="JQG95_000562"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="JQG95_000562"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY5887235.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="JQG95_000563"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="JQG95_000563"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY5887236.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="JQG95_000564"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="JQG95_000564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY5887237.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="JQG95_000565"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="JQG95_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY5887238.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSSEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQELAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHTILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="JQG95_000566"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="JQG95_000566"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY5887239.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JQG95_000567"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="JQG95_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY5887240.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="JQG95_000568"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="JQG95_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY5887241.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="JQG95_000569"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="JQG95_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY5887242.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="JQG95_000570"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="JQG95_000570"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY5887243.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="JQG95_000571"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="JQG95_000571"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY5887244.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="JQG95_000572"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="JQG95_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY5887245.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGTIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="JQG95_000573"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="JQG95_000573"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY5887246.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JQG95_000574"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="JQG95_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY5887247.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JQG95_000575"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="JQG95_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY5887248.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JQG95_000576"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="JQG95_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY5887249.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="JQG95_000577"
     CDS             complement(40421..41815)
                     /locus_tag="JQG95_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887250.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JQG95_000578"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="JQG95_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY5887251.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="JQG95_000579"
     CDS             complement(42158..43444)
                     /locus_tag="JQG95_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5887252.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="JQG95_000580"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="JQG95_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="JQG95_000581"
     CDS             complement(44451..45221)
                     /locus_tag="JQG95_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY5887253.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45693..47207
                     /locus_tag="JQG95_000582"
     CDS             45693..47207
                     /locus_tag="JQG95_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY5887254.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47238..48380
                     /gene="caiA"
                     /locus_tag="JQG95_000583"
     CDS             47238..48380
                     /gene="caiA"
                     /locus_tag="JQG95_000583"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY5887255.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48509..49726
                     /gene="caiB"
                     /locus_tag="JQG95_000584"
     CDS             48509..49726
                     /gene="caiB"
                     /locus_tag="JQG95_000584"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY5887256.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49800..51353
                     /gene="caiC"
                     /locus_tag="JQG95_000585"
     CDS             49800..51353
                     /gene="caiC"
                     /locus_tag="JQG95_000585"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY5887257.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51462..>51658
                     /locus_tag="JQG95_000586"
     CDS             51462..>51658
                     /locus_tag="JQG95_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HAY5887258.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWD"
BASE COUNT        12643 a        13999 c        13354 g        11662 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggattagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcagcgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 gcaccgccgc ctgctcatga cgaactaata catgatcaat accacccacg gtatgcaatg
     3361 catcgtaaat atcaaggact gcgcctccgg gataaccgaa tacttgttta acgccctgat
     3421 cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc tgcctcactg
     3481 tttgacggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag caattggcag
     3541 ccagagacat aaacgcaggg taataagaat aaacaggagg aaaggtgaaa agaaacgata
     3601 aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca caacttacag
     3661 caatttctct atttttttaa ttaatcctaa aatagagagt ccacacattc agaataaaat
     3721 tctacattca ataaaaaatg cagaataaac cagacattca tgtctggcct attctgcatt
     3781 cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc ctttatcgcg
     3841 cccggcagct tcatgccaag agagataaca ggttctgctg ttttgtttta acggcagcgg
     3901 taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg gcgcaatagc
     3961 gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc cctgatacgc
     4021 gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg cgaaacgatc
     4081 gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg ggcctttaat
     4141 tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg gcacgctggt
     4201 aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct gataacgcag
     4261 ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat ttggcgcaat
     4321 ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc tgcaaacaca
     4381 aagatgaaat acacgttcac tgctcgcggg ttcaaaacct gaaccaggca attcattttg
     4441 tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag cagtcggttg
     4501 aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca ggcgtgcaac
     4561 agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg taatattttg
     4621 ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc gtagctgtgg
     4681 cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac ttaactccac
     4741 tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg aagaatatga
     4801 tttatcatgc atataaaata agaaaaagca aaatgagtaa aattcggaaa acataaagat
     4861 acgggttctg ataaaaacag aatccattgc cgtggaatga gtcatttacc acagcataat
     4921 aatccataat gtttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgatcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caatgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggc ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cggtctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaaccgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaagacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctagtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgatgaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agttcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagccacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggaac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggttgaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cactatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtac aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccagc gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    45361 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt ttaatattgt
    45421 tttatagatc accaatattg aaagtcagat gctatttttt tacccacaaa agaatataac
    45481 aaactgatta ataaccaaaa ttaacgaaaa acgccctcta ttcactcgaa acaaaaatgt
    45541 gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    45601 cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    45661 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    45721 tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt tggcttacag
    45781 tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    45841 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    45901 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt agcaccgcca
    45961 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    46021 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacaggag
    46081 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    46141 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    46201 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggta
    46261 ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    46321 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    46381 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    46441 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    46501 tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    46561 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    46621 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tacgccatcc
    46681 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    46741 gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc ggtagtaaca
    46801 ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    46861 tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    46921 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    46981 tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg ctggtgcgta
    47041 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    47101 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    47161 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    47221 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    47281 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    47341 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    47401 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    47461 tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg ggcgggttca
    47521 ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct ttccgcggca
    47581 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    47641 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    47701 ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    47761 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    47821 ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgatg
    47881 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    47941 aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg gcgatgtgcg
    48001 cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag gctattggtc
    48061 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    48121 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    48181 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc gcaatgcagg
    48241 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgacctgc
    48301 gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg ggtcgtgcgg
    48361 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg cgtcttatca
    48421 ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc ggcagtcatg
    48481 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcggacc
    48541 gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt ttgccggaca
    48601 aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct gggccgacac
    48661 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    48721 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    48781 cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    48841 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    48901 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    48961 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    49021 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    49081 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    49141 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    49201 ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga tggaactggt
    49261 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    49321 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    49381 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    49441 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    49501 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    49561 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    49621 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    49681 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    49741 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    49801 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    49861 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    49921 ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg gggattcgca
    49981 aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt tgctggttcg
    50041 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta cgcgaggaaa
    50101 gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattttatc
    50161 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    50221 atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa aatcaacaac
    50281 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    50341 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    50401 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac atgacggtaa
    50461 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    50521 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gcgcagaagt
    50581 accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg gtgcagccgc
    50641 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    50701 agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    50761 tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga cgctggccgt
    50821 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    50881 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    50941 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    51001 ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc gatcgccgct
    51061 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    51121 ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg attcgcgatg
    51181 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    51241 ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    51301 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    51361 gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca ttttttgcag
    51421 ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    51481 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg aatgctattg
    51541 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    51601 tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc tgggattt
//
LOCUS       DADLCQ010000011        48311 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000011 DADLCQ010000000
VERSION     DADLCQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.011"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            187..480
                     /locus_tag="JQG95_000587"
     CDS             187..480
                     /locus_tag="JQG95_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5887259.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQG95_000588"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="JQG95_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY5887260.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="JQG95_000589"
     CDS             complement(1922..2374)
                     /locus_tag="JQG95_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY5887261.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /locus_tag="JQG95_000590"
     CDS             complement(2427..3761)
                     /locus_tag="JQG95_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5887262.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="JQG95_000591"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="JQG95_000591"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY5887263.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVMIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQG95_000592"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="JQG95_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY5887264.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQG95_000593"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="JQG95_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY5887265.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQG95_000594"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="JQG95_000594"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY5887266.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQG95_000595"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="JQG95_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY5887267.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQG95_000596"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="JQG95_000596"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY5887268.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQG95_000597"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="JQG95_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY5887269.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQG95_000598"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="JQG95_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY5887270.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="JQG95_000599"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="JQG95_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY5887271.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="JQG95_000600"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="JQG95_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY5887272.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="JQG95_000601"
     CDS             complement(15085..15582)
                     /locus_tag="JQG95_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY5887273.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="JQG95_000602"
     CDS             complement(15579..15941)
                     /locus_tag="JQG95_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY5887274.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="JQG95_000603"
     CDS             complement(15931..16278)
                     /locus_tag="JQG95_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY5887275.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VVVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="JQG95_000604"
     CDS             16387..16800
                     /locus_tag="JQG95_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887276.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18313)
                     /locus_tag="JQG95_000605"
                     /pseudo
     CDS             complement(16847..18313)
                     /locus_tag="JQG95_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="JQG95_000606"
     CDS             complement(18324..20039)
                     /locus_tag="JQG95_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY5887277.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="JQG95_000607"
     CDS             20206..21099
                     /locus_tag="JQG95_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5887278.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="JQG95_000608"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="JQG95_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(21910..23527)
                     /locus_tag="JQG95_000609"
                     /pseudo
     CDS             complement(21910..23527)
                     /locus_tag="JQG95_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            23822..24496
                     /locus_tag="JQG95_000610"
     CDS             23822..24496
                     /locus_tag="JQG95_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5887279.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24535..26196)
                     /locus_tag="JQG95_000611"
     CDS             complement(24535..26196)
                     /locus_tag="JQG95_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY5887280.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="JQG95_000612"
     CDS             complement(26392..26820)
                     /gene="ibpB"
                     /locus_tag="JQG95_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY5887281.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="JQG95_000613"
     CDS             complement(26932..27345)
                     /gene="ibpA"
                     /locus_tag="JQG95_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY5887282.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            27650..27982
                     /locus_tag="JQG95_000614"
     CDS             27650..27982
                     /locus_tag="JQG95_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY5887283.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27984..29240)
                     /locus_tag="JQG95_000615"
     CDS             complement(27984..29240)
                     /locus_tag="JQG95_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY5887284.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTNTWTPDSQWLVFDV
                     RPSGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDET
                     WHYDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDH
                     VMHQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINR
                     AYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPA
                     PPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQG
                     GEPRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSAD
                     AVVFSPDGQWLAWMEGGQLWITETDR"
     gene            29305..30405
                     /gene="cbrA"
                     /locus_tag="JQG95_000616"
     CDS             29305..30405
                     /gene="cbrA"
                     /locus_tag="JQG95_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY5887285.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30366..31703)
                     /locus_tag="JQG95_000617"
     CDS             complement(30366..31703)
                     /locus_tag="JQG95_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887286.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVVTLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="JQG95_000618"
                     /pseudo
     CDS             complement(31778..32926)
                     /gene="dgoD"
                     /locus_tag="JQG95_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32923..33540)
                     /locus_tag="JQG95_000619"
     CDS             complement(32923..33540)
                     /locus_tag="JQG95_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY5887287.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33524..34402)
                     /locus_tag="JQG95_000620"
     CDS             complement(33524..34402)
                     /locus_tag="JQG95_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY5887288.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     VVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINGFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="JQG95_000621"
     CDS             complement(34399..35088)
                     /gene="dgoR"
                     /locus_tag="JQG95_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY5887289.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35366..36022
                     /locus_tag="JQG95_000622"
     CDS             35366..36022
                     /locus_tag="JQG95_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887290.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="JQG95_000623"
     CDS             complement(36068..36880)
                     /gene="yidA"
                     /locus_tag="JQG95_000623"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY5887291.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36994..37401)
                     /locus_tag="JQG95_000624"
     CDS             complement(36994..37401)
                     /locus_tag="JQG95_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY5887292.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="JQG95_000625"
     CDS             complement(37632..40046)
                     /gene="gyrB"
                     /locus_tag="JQG95_000625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY5887293.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40075..41148)
                     /gene="recF"
                     /locus_tag="JQG95_000626"
     CDS             complement(40075..41148)
                     /gene="recF"
                     /locus_tag="JQG95_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY5887294.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="JQG95_000627"
     CDS             complement(41148..42248)
                     /gene="dnaN"
                     /locus_tag="JQG95_000627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY5887295.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="JQG95_000628"
     CDS             complement(42253..43656)
                     /gene="dnaA"
                     /locus_tag="JQG95_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY5887296.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43953..44033
                     /locus_tag="JQG95_000629"
     CDS             43953..44033
                     /locus_tag="JQG95_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887297.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44263..44403
                     /gene="rpmH"
                     /locus_tag="JQG95_000630"
     CDS             44263..44403
                     /gene="rpmH"
                     /locus_tag="JQG95_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY5887298.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44420..44779
                     /gene="rnpA"
                     /locus_tag="JQG95_000631"
     CDS             44420..44779
                     /gene="rnpA"
                     /locus_tag="JQG95_000631"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY5887299.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44743..45000
                     /gene="yidD"
                     /locus_tag="JQG95_000632"
     CDS             44743..45000
                     /gene="yidD"
                     /locus_tag="JQG95_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY5887300.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45003..46649
                     /gene="yidC"
                     /locus_tag="JQG95_000633"
     CDS             45003..46649
                     /gene="yidC"
                     /locus_tag="JQG95_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY5887301.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46755..48119
                     /gene="mnmE"
                     /locus_tag="JQG95_000634"
     CDS             46755..48119
                     /gene="mnmE"
                     /locus_tag="JQG95_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY5887302.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11992 a        13061 c        12206 g        11052 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcacgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tgatgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgcagggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caagaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagtaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 gcggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa acttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccacc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttgg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcaaaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat tcccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgatactca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaac atcgggtttt gcagcaagat ggcaagaccc accacaatcc
    23461 cggcgaaggg aaacagcagc actggcgtga acatcgcgcc gccaaagcgt tgaatttgac
    23521 tgagcatttc cacatcctct tttctcaatt ctgtaagtag gaagcctgat tggttatgcg
    23581 gcagacctgg agcgaggata ggaagatgcg atcggcagaa catgacgacc caatgggatt
    23641 cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct acgttttcgc gaaaaagtag
    23701 acatctgtga gcacaggtac acagcatcga acacatcttt aaaaaaaaga tttttttcaa
    23761 tcgattaagc agaacttgtg ggcgcattac ccgtgcttgc aggcaaaaaa gagagctaga
    23821 gatgatctac aaaagcattg cggagcggtt aagaattcga cttaattccg cggatttcac
    23881 gctaaacagc cttcttcccg gtgaaaaaaa gctggcggaa gagtttgcgg tatcgcggat
    23941 gactatccgt aaagccattg acctgctggt agcgtggggg ctggtggtcc gccgccacgg
    24001 cagcggcact tacctggtgc gcaaagatgt gctgcatcaa accgccagcc tgaccggact
    24061 ggtggaggtg ttaaaacggc agggaaaaac ggtcaccagc caggtgctga tttttgaaat
    24121 catgcctgcg cctccggcca ttgccagcca gttacggatt caaatcaacg agcagatcta
    24181 cttctcccgt cgcgttcgtt ttgtggaagg gaaaccgcta atgctggaag acagctatat
    24241 gccggtaaaa ctgttccgta acctttcgct gcaacatctg gaagggtcga agtttgaata
    24301 tattgaacaa gagtgcggga ttttgattgg cggtaattat gaaagcctga tgccggtgct
    24361 cgccgataga ctgctggcgc ggcaaatgaa ggtagcggaa cacacgccac tgctgcggat
    24421 cacctcgttg tcatatagcg agagcgggga gtttttgaat tattcagtga tgttcagaaa
    24481 tgccagcgaa tactaggtgg agtaccattt acggcgactc cacccggaaa aaagttaacc
    24541 gatactccag aagagcaccg ccagtaattg gggggtgata atgcgcagga acatcactaa
    24601 cggatagaca gtggcgtaag agagcgccgc cgcaccactg gttggatgaa gattattagc
    24661 aaacgccagc gccggaggat cggtcatgga acctgccagc atcccgcaca tggtcaggta
    24721 attcattttg gctaacatcc gcgccagaat gccaacagta atcagcggaa cggcggtgat
    24781 cagggcacca taaccaatcc agcttagccc ttcgccattg accagggtat tcacaaaatc
    24841 cccaccagat ttcagaccaa cgaccgagag gaacagcacg atccccagct cccgcagcgc
    24901 gaggttggca cttggcggca taaaccagta cagcttgccg atactgccga tacgcccgag
    24961 gatcaacgcc ataatcagcg gaccgcccgc cagccccagt ttcaacgcgg ccgggaatcc
    25021 tggcacaaag acgggaatag aacctaacaa tacgccaagc ccgatgccaa taaacactgg
    25081 cagcatctga acctgttgca gtttttgctg cgcattcccc agcacattgg caacggcatc
    25141 aattgcggac ggacgcccca ccaggttgag gatatcgccg aactgcaggc tgatatcgcc
    25201 actggcgacc agttcgaccc cggcacggtt caggcgcgag ataacaacgt catagcgttc
    25261 tttaaagtgc aggtcgcgaa tacgttttcc gagcacgttt tcattggtga ccaccacacg
    25321 ctcgacgcgc aaatcagtgc ctttcgtaga cagcgaggta tcgacctcct gaccaatcac
    25381 cagttgcgca ttatgtaaat ccgctggctg ccccaccaga tgcagcaaat cgcccagttg
    25441 gataatggta tctggcgaag gtacttttag ggtttcttct cgtttcagac gcgagcagat
    25501 aattttgtcg ccgttgagaa tcggtacatc tttaatggct aaatcatgca ggttagggtt
    25561 ctcaacgcga atattgatag tcttgatcaa cgcgccgcca ttggtgcgtg aagactcgtg
    25621 ctgctgagct tctgtctcga cattgacgcg gaaaataacc cgcaacatcc acatggtgaa
    25681 caaaatcccg caaatgccga atggatacgc catcgcgtaa ctcatcccca tctgatcgac
    25741 catttccatt ggtgtaccca ggtcgcgcaa gatctgctgc cctgccccca gcgctggcgt
    25801 attggttacc gcaccggaga aaatccccag cactaccggc agtggaatat caaacagttt
    25861 atgcaggatg gcggtaacca gaccaccgat gatgacgatc agaacagcaa acaggttgag
    25921 gcgtaatccg gagacgcgca atgaggcaaa gaagcccggc cctacctgaa tcccgatggt
    25981 ataaacgaac aggatcaggc caaactcctg aataacatgc agcatatcgc tacttaatgt
    26041 catccccgcc tgagaaacaa aatggccgac gatgatccca ccaaacagca cgccgccaat
    26101 acctaatcct atgccgcgaa atttgacgtt gccgataaac aaaccgacga ctgccaccaa
    26161 agccagaata ctgaccgtta atgctatatc actcatcgtc catttctctt gagataacat
    26221 tttaattgct acggattatg gctgaatcgg tgaagcagat ttgccttata gcgcacatta
    26281 tcacgggaaa gaacgtgccg aaatatctta aacagtcgaa atttaacgca ttgttgaaga
    26341 agaaagacaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    26401 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    26461 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    26521 agccagcgta aaactcaggc taaatggctg attcataagc ccttgatgca gccatttttt
    26581 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    26641 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    26701 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    26761 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    26821 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    26881 ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga attagttgat
    26941 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcaa
    27001 accatttacc aggttagccc cacgaacatg aatgttctca gctaactgga atttgcgttc
    27061 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcgtg
    27121 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    27181 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    27241 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    27301 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    27361 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    27421 aggcgcgtcc tcatcgacta cgatgtaaaa tgggtctgga aatgaacttt tcaagcctta
    27481 tcagcggact tttttttgca gtttatggtc tattgcatag actgaggggg cagcacgaaa
    27541 cgttaaagtg cgatagccgc cacagtgcga cgtaatgatg atgttatttt cagcaaggga
    27601 tcgctataac tcatcaggcc aacacccacg atcaacagat gaatttatta tgataagaaa
    27661 tgtgttgtta gcgttcatga tatgcagcgg aatgacatta ctcggaggat gctccagcgt
    27721 gatgtcccat accgggggga aagaaggaac gtatcccggc acccgcgcca gcgccaccat
    27781 gattggcgac gatgaaacca actggggcac taagtcgctg gcgattctcg atatgccgtt
    27841 taccgctgta atggacacgc ttttactgcc gtgggatgtg ttccgcaaag acagctcggt
    27901 gcgctcgcgc gttgaaaaaa gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc
    27961 ggccagaatg cctgataact gatttagcga tcagtttcgg tgatccacag ctggccacct
    28021 tccatccacg ccagccattg accatccggc gagaagacca cggcgtccgc agaaggtgga
    28081 ttggcgtggt tttcggttaa atactcaacc tcgccgcttt gcgcatgggc acaaacaatt
    28141 cgattatcca gcacaaagcc caaccattct cctgacggat gccagttaaa tgcagactga
    28201 atatccgttt tgttatgggt taacttgcgc ggttcgccgc cctgtggcga gataagccac
    28261 agttgcacaa tgccgttatc atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta
    28321 cagcgcaccc agtggcgcgg gacgttgact aaccccggat aagcccgatg atgggtaaag
    28381 gttaaacgtc gctgcacgac gccacgcggt ggcgcgggta gggttgtttc cgttccactt
    28441 aacggcgcat cacctgccga tttccagcta gcttcatctt gcggtaactc aacgataaat
    28501 agctccggca ctttctcgcc ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga
    28561 tttcctaccc atccttcttc ataagcacga ttgatttcat cactgccagg ctgcggcgtg
    28621 ggcgtggttt tactcaccag cacacaccag tggctaccgc tgtattcacg cggatgttgc
    28681 ttttgtacgt tgaccgggcc aaacggtgca gcaaccccga cgtttcgcaa atccagcgcc
    28741 ggatcgagtt gatgcattac atggtcgtta taggtaaagc tccccctttc accgttcggg
    28801 ctaaagacat gcacatggct gccaccgcgc agcgctcctg gcgtatacgg cgcggtaata
    28861 tccattgcat cgagattgct caccttgccg ccttcagcaa tgactccgcg tcgatgatgg
    28921 aagtcgtaat gccatgtttc atcaggattt tcagggccgt gaataaagac atatttctct
    28981 gacttcggat gaacggtcac cacgccgacg tgtgcgccct gtgacgcgcg atagataacc
    29041 tcgacctcgc ctgtatggat attcacacgc tcaatggtct cgccggtaaa cgacgcccca
    29101 gaaggacgca cgtcaaatac cagccactgg ctgtcgggcg tccaggtatt ggtattggta
    29161 ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac
    29221 aaaacggttc ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc
    29281 atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat tggcctcggc
    29341 ccggcagggt cggcgttggc acgaaagtta gccggcaaaa tgcaggtgat cgcgctggat
    29401 aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg
    29461 gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaat
    29521 ccacagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag
    29581 cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc
    29641 gccagcgttg aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa
    29701 tggcatgtca tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc
    29761 ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt
    29821 aaatatgtcg ctatccagca gtggttcgca gagaaacatc cggtgccgtt ctactcctgc
    29881 atctttgata atgcgataac tgactgttac tcatggagta tcagcaaaga tggttatttt
    29941 atctttggcg gtgcctatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag
    30001 aaaatgagcg cctttcagtt ccagtttggc aaagcggtga aaagtgagaa atgcacggtg
    30061 ttgtttccct cacgctggca ggattttgtc tgtggtaagg acaacgcctt tctgattggt
    30121 gaagcggcgg gatttatcag cgccagctcg ctggagggga ttagctatgc gctggatagc
    30181 gcagagattc tgcgtgcggt gttcctgaaa cagccggaga agagcaacgc cgcttactgg
    30241 cgcgccacac acaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg
    30301 accgcaccgg ctttaagaaa gtggattatg cgcagtggtg tggcgcatat tccacagttg
    30361 aaagattatc caacgcgctt cacatcaccc accagcagga tgtaagagag cgcgccaatc
    30421 aacgcgacgg cggagatata aaccagtgca ggcgcgaaac cgtaaccctg cgccaggtac
    30481 cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg
    30541 gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca
    30601 aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttggtg
    30661 tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc
    30721 gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg
    30781 acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg
    30841 attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgcagaa
    30901 gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag
    30961 tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga
    31021 ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc
    31081 ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg
    31141 aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc
    31201 agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg
    31261 aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt
    31321 atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccttggaa taaagtcacc
    31381 actgaccaac caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac
    31441 caaccgccgg ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc
    31501 atttccgctt tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaagtta
    31561 gcgcggtcaa cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc
    31621 cgacgccccg gctttgctgc attaacggga atatccatag cgagctttct ccagattttg
    31681 ggcatagcga agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc
    31741 tgttttagtg cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat
    31801 cttcatgacg ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt
    31861 tagcttcgtc gatttccaca cctaagcccg gtttcgttaa cggtttaaag aagccgccaa
    31921 ccatgctgaa gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat
    31981 gaatgcccat actttgttac tgaagtaccg cgttatagga aacaaagtcg atatgcaggc
    32041 aagccgccag tgcaatcggt ccgaacggac agtgcggcgc aagggtcaca tcataggctt
    32101 ctgccattcc agcaattttg tagcattcgg taataccgcc tgcatgggag agatccggtt
    32161 gcagaatcga aataccgcct gcctccagca cgcgtttaaa atcgaaacgt gagaacatgc
    32221 gctcacccgc cgccagtgga atatgcgttt gtgccgccaa tttcgggtag tattcggctt
    32281 gttccgccag caccggctct tcaataaaca gcgggcgata cggctccagt tctttaatca
    32341 gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat
    32401 tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt
    32461 tatcaattag ccccagttct tcacaaccgt tcagtttgaa ggtatcgaag ccgatttcgc
    32521 gcagggtttt aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg
    32581 ctttaatttt gtcgcgaacc aggccgccca tcagttgcca gactggcgca ttcagcactt
    32641 tgcctttgat atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac
    32701 cgccacgata aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgaaggat
    32761 cctgaccaat caaatagtca cccagctcgt gaactgccgc ttccaccgta cgggcgcggc
    32821 cttcaatcac gggctcgccc caaccgacca cgccttcatc ggtttcaatt ttcaggaaca
    32881 tccagcgggg aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg
    32941 atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg
    33001 ataaagatcg ctgcctaagc ccgccccaac acaacctgcg tctatccact gcgccaggtt
    33061 ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc ggtggtaata ccgcttttaa
    33121 cgctttgatg tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc
    33181 cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaaa cggtcatgcc
    33241 gtagcccacc gcgcggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca
    33301 gcccatcctg gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa
    33361 cgccttgtcg ccatacgcat caacgatggc gggaatgctc tgctcccatt gtggggaatt
    33421 cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc
    33481 ctcgtcgggc gtaatgccgc gcaaaatggc gatcagcggg agtttagttt gccactgcat
    33541 gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gccacgtcgc
    33601 aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg
    33661 tgatggcgtg ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca
    33721 aaccagagag aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc
    33781 gaacttcaaa aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg
    33841 cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta
    33901 acagtaaatg atgtaattca ccggtcatca cggtgcgaaa accgttgatt tgctggctat
    33961 cggcctgcac ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag
    34021 ctcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag
    34081 agacgcataa tccgggaata atccagatat tgtcgccaac agacgttaat tgttcgccaa
    34141 tagacgaaaa acgggcagga acagataaat acggagcaac tttccagccg acattgctgc
    34201 cgaccattcc tgccatcacc actggcgtat tttcttcacg ccagtcggtc gtgacttctg
    34261 ctaacaccac agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc
    34321 tctccaggca gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa
    34381 ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcaatcat
    34441 ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc cgtatcgcat cgaacagcgc
    34501 cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa
    34561 aaccgcccgc tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac
    34621 cgactgcagc accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg
    34681 attgttggca atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaggtcgc
    34741 gcgctctgct gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc
    34801 actgataagc cgtgggtcgt agtcgttttc cagcacccat tgcagtacgt cagtgtcgag
    34861 gtaattccac tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag
    34921 ccgcttcgcc atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc
    34981 ttcacagagc tccgcctcag ccggaagtgg cgagccaggt acgtatttgc cgtgaacgat
    35041 ctgtttaccc agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg
    35101 tgctctgtat gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt
    35161 accagcgtga tgacgttcgc gtttgccgcg cgcgtaatgt agtacaaact tatattgtta
    35221 tgctacaatt tagatcaaaa aaagaacaat acataaaaaa atgacatgcg tcgggcagaa
    35281 atctgaaaag ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct
    35341 tagctactcg tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc
    35401 cggtttattc ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca
    35461 taccgcaaag ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc
    35521 gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta
    35581 ccttatcact gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg
    35641 gcacaatatt caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt
    35701 taccggcaca atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac
    35761 gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg
    35821 cacaatccat gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc
    35881 atttaaagtg cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc
    35941 agcattactg ccagtgactg ttacactgga catcattact gcaccgctgc aatttctggt
    36001 tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc
    36061 ttacagatta attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttgg
    36121 attttgtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    36181 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    36241 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    36301 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca
    36361 cttcctgcgg aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca
    36421 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaattg
    36481 tggcaacgaa ggattcatgc accgtgtagt agctgatatc gcggttagcg gtgtacagcg
    36541 tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    36601 gatagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    36661 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    36721 ggtagttgtg cacacctgca tacggacgac ccgtcgttag cacgacattc acgccacggg
    36781 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    36841 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccaa ggtagattag
    36901 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    36961 ggatgcaacg cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag
    37021 ttccatgcct gcggaaagca gatcgttatt ggcttgtgct gacacttcac cttgcggtga
    37081 gagcgcatca ataatcttcg gcaattgctc tgccagtaaa ctggaagccg tactggtatc
    37141 cacgccaagt ttttgcccga gatcggacac cgcatttgtg ccgagcgccg actccagttg
    37201 ctcgccgcta actgattgat ttctctgttg attactcaac caggttgaga gaatggcccc
    37261 taagccgcca ctttgcagtt tttccagcag cacctgaatg ccaccctgct cctcaaccca
    37321 acttaaaatc gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc
    37381 aaaaagcccc atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagatct
    37441 gcgggatgat aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc
    37501 ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgcaaaa
    37561 tcaatatatt gcaggagtct cgtaggacta ataagcgtag cgcatcaggc acgctcgcat
    37621 ggttaacgcc attaaatatc aatattcgcc gctttcaggg cgttctcttc aataaacgca
    37681 cgacgcggtt ctacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca
    37741 tccttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc
    37801 tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc acgacgggac
    37861 tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca
    37921 cgttcgataa acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata
    37981 cgacgatatt cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg
    38041 tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca
    38101 aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc
    38161 cagcgggtaa cggtctgctc atcagaaagg tcagcttccg tcaacgtcgg ctgatagata
    38221 agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc
    38281 gcgttgtact cagataccag tttctctaac gcttcgccag ccaatgccgg tgcactggcg
    38341 ttggtgtgca gcgttgcgcc gtccagcgcg atagagatct ggtactgatc catcgcttcg
    38401 tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg
    38461 atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc
    38521 agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa
    38581 cgcagtttgt ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc
    38641 gtcgccactt cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttaaggatt
    38701 ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg
    38761 cccgcggagt ccccttccac caggtacagt tcggaaagcg ccggatcgcg ttcctggcag
    38821 tctgccagtt tgcccggcag gccagctaag tcgagcgcac ctttacggcg ggtcatttca
    38881 cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt
    38941 ttcgcatcag ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca
    39001 accgccgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg
    39061 tccggcactt ttacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg
    39121 ctgactttgg cttttttgct gtagccttct ttgtccatgt aggcattcag ggtacgggtc
    39181 atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta
    39241 aagcagtaga tgttttcctg gaagccatcg ttccactgca gcgccacttc aacgccaata
    39301 ccgtcttttt cggtggagaa gtagaagata tttgggtgga tcggcgtttt gttcttgttc
    39361 agatattcaa cgaacgcttt gatgccgcct tcatagtgga agtggtcttc tttgccgtca
    39421 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
    39481 ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag
    39541 aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt
    39601 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctgaataac cagctccagt
    39661 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
    39721 taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
    39781 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
    39841 acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
    39901 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
    39961 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
    40021 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
    40081 tgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
    40141 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
    40201 aagcaacccg cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
    40261 ccgccgcccg ctttcacgag tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa
    40321 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
    40381 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
    40441 acgttccagc acctcagcgt actctgtctc tttctcccag ccgcgctgga aagagaaagt
    40501 cagagaaaac tcagggagaa attgcttaca ggtatcatcc atatcggccg cgataccggc
    40561 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
    40621 cgggcgtagc tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
    40681 tcgcttgaga ttgctccagg cggtgaaaaa tccaggttcg ttgtgaaagc atccccagtc
    40741 gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat
    40801 caactgcatc ggcatcaggt gcgccagttc cgcgacttta tgcccgtccg taccgtcaat
    40861 acggactttg ctgtcgccct gtttgtcttt ggttaagcca atcgccgtct cgcgctcttc
    40921 gccctgtaat cgcccgtgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat
    40981 ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt
    41041 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc
    41101 ggtttcaata ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt
    41161 ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc
    41221 gaatcggtca gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca
    41281 tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct
    41341 tccgcttctt cctgttccgg gttgttggca gtaattttca gctggttttc gctgacatac
    41401 aggcgcacac cgcggaattt ctcgttagag agaatcgccg cgcgagcaaa cgcctgcttg
    41461 agcagatcgc agcctgcttc cagatgtttg tccgggttct tcggcagaac gcgacggtaa
    41521 tccgggaagc gaccatccac cagtttcgag gtgaaaataa agtcgccaac gtgggcacga
    41581 atgttattgc tgccaatctg cacacgcagc agattgtcgc cgccgtcgag catacgcatc
    41641 agttcaatca cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt
    41701 ggcattgaac agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct
    41761 tcggtttcaa acagcatacc gtttaagtaa tagcgaacat cctgatgcgc catcgaaaac
    41821 tgggttgctt caatcagacg cttcatcgtc gcctgcggca gggtaaattc gacttcgctc
    41881 tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta
    41941 cgcccggagc gcaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca
    42001 ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg
    42061 tgtggctgaa ccagcgcaac gcgtgccacc atttccatct cgagatcagt accggtcagc
    42121 gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga
    42181 ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta
    42241 aatttcatag gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc
    42301 gtggctctct tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc
    42361 acgcccacca aacgcatcgc caatctctgg cagactgtgg ttagtcagct ctttcgctag
    42421 cgccatcgcc atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc
    42481 cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag
    42541 tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc
    42601 ccgtccggta aagttggcgt tagcaataac gcggttcagc gccccttcca gctcacgtac
    42661 gttagatcgt agacgcttgg cgataaagaa cgccacttca cccggcaaac gaatgtcatt
    42721 ttcgtcggcc tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgatcgc
    42781 cacagtcagt ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt
    42841 cggatagcga tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt
    42901 gtggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag
    42961 cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc
    43021 tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt
    43081 gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc
    43141 gccataaagg aacaacgggt tataggcacc gccagggtta tccgccacct ggcaagccgc
    43201 cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt
    43261 tacgttagaa cgataggtcg gttctgccgg ggccgggacg ttatcccaac ctgagcgcgt
    43321 agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct
    43381 cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg
    43441 tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc
    43501 ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag
    43561 ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa
    43621 tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt
    43681 cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt
    43741 gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga
    43801 tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc
    43861 cctatttatc cacaggactt tccagaactc gctaagtgta aacgatcctg ccgcgaggcg
    43921 ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc
    43981 gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca
    44041 cccggcgcgc catgctggtt tccactggtg tgaggtcgta cattttccct gcgaaaaggt
    44101 gcggaaaagc gcggtaaata aggaaagaga attgactccg gtgtgtacaa ttattacaat
    44161 ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc
    44221 aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa
    44281 ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat
    44341 ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag
    44401 taataaagct aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac
    44461 tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac
    44521 cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa
    44581 aaacgttcga cgcgcccatg aacgcaatcg gattaaacgc ctgacgcgtg aaagcttccg
    44641 tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc
    44701 cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg
    44761 cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta
    44821 ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc
    44881 aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct
    44941 ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa
    45001 cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct
    45061 ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa
    45121 cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac
    45181 tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg
    45241 agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgt
    45301 tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc
    45361 cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc
    45421 tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca
    45481 cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg
    45541 tgcagaacgc tggcgagaaa ccgctggaaa tctcctcgtt tggtcagttg aagcaatcca
    45601 tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg
    45661 gcgcggcgta ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg
    45721 ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt
    45781 tcgcgacggc gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg
    45841 gtaacggcat cgtcgctatc ggctataaat ctcagccggt actggttcag cctggtcaga
    45901 ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg
    45961 ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc
    46021 tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca
    46081 tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca
    46141 tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg
    46201 acaaacagcg tatcagtcag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc
    46261 tgggcggctg cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc
    46321 tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg
    46381 cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga
    46441 agatgtcgcc gaccactgtg accgacccga tgcagcagaa gatcatgacc tttatgccgg
    46501 tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtactgtac tatatcgtca
    46561 gcaacctggt aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc
    46621 tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt
    46681 cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa
    46741 gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac
    46801 gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc ccgtgaagtt gccgaaaccg
    46861 tgctgggtaa actgcctaag ccgcgctacg ccgattatct tccgtttaaa gacgccgacg
    46921 gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg
    46981 aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac
    47041 gcattctgac cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt
    47101 ttcttaacga taaacttgac ttagcccagg ccgaggcgat tgccgatctt atcgacgcca
    47161 gttcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg
    47221 ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt
    47281 tccctgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag cttaatgacg
    47341 tgatggccga tctcgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag
    47401 ggatgaaagt agtgattgcc ggacgtccta atgccggtaa atcgagcctg ttaaacgcgc
    47461 tggcgggccg tgaagcggca atcgtaaccg atatcgccgg aaccacgcgt gacgtgctgc
    47521 gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacactgcc gggctacgtg
    47581 aggccagtga cgaagtggaa cgtattggta tcgagcgcgc gtggcaggaa attgaacagg
    47641 ccgaccgcgt gctgtttatg gtcgatggca ccacgacaga cgccgttgat ccggcagaga
    47701 tctggccgga atttatcgcc cgtctgcccg cgaaactgcc gatcaccgtg gtacgcaata
    47761 aagccgatat caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc
    47821 gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca
    47881 tgggctttga caccaatatg gaaggcggct tcctggcgcg ccgtcgccac ttacaggcgc
    47941 tggaacaggc agcggagcat ttgcagcaag gtaaagcgca actgttggga gcctgggcag
    48001 gtgaactgct ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg
    48061 aatttacttc agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac
    48121 cgcgcttacg aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc
    48181 tgttatgcct caaccctttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    48241 aaagcattta atactgctcc ccgaacgatt gtgattcgat tcacatttaa acaatttcag
    48301 aatagacaaa a
//
LOCUS       DADLCQ010000012        47945 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000012 DADLCQ010000000
VERSION     DADLCQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.012"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1227
                     /locus_tag="JQG95_000635"
     CDS             <1..1227
                     /locus_tag="JQG95_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5887303.1"
                     /translation="EFELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLEN
                     HPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYM
                     IIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGN
                     IALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELV
                     YVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDK
                     NFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPE
                     TVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAE
                     KYLLAQFGVDIGEAAQ"
     gene            1224..2189
                     /gene="yggF"
                     /locus_tag="JQG95_000636"
     CDS             1224..2189
                     /gene="yggF"
                     /locus_tag="JQG95_000636"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY5887304.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPHTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2211..2720
                     /gene="fumE"
                     /locus_tag="JQG95_000637"
     CDS             2211..2720
                     /gene="fumE"
                     /locus_tag="JQG95_000637"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY5887305.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2717..3430
                     /locus_tag="JQG95_000638"
     CDS             2717..3430
                     /locus_tag="JQG95_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY5887306.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3402..4084)
                     /locus_tag="JQG95_000639"
                     /pseudo
     CDS             complement(3402..4084)
                     /locus_tag="JQG95_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
     gene            complement(4078..4755)
                     /locus_tag="JQG95_000640"
     CDS             complement(4078..4755)
                     /locus_tag="JQG95_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY5887307.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4743..5126)
                     /locus_tag="JQG95_000641"
     CDS             complement(4743..5126)
                     /locus_tag="JQG95_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY5887308.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5142..5450)
                     /locus_tag="JQG95_000642"
     CDS             complement(5142..5450)
                     /locus_tag="JQG95_000642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887309.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5451..6029)
                     /locus_tag="JQG95_000643"
     CDS             complement(5451..6029)
                     /locus_tag="JQG95_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY5887310.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6052..6483)
                     /locus_tag="JQG95_000644"
                     /pseudo
     CDS             complement(6052..6483)
                     /locus_tag="JQG95_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6855..7874
                     /gene="epd"
                     /locus_tag="JQG95_000645"
     CDS             6855..7874
                     /gene="epd"
                     /locus_tag="JQG95_000645"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY5887311.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7924..9087
                     /gene="pgk"
                     /locus_tag="JQG95_000646"
     CDS             7924..9087
                     /gene="pgk"
                     /locus_tag="JQG95_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY5887312.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9302..10381
                     /gene="fbaA"
                     /locus_tag="JQG95_000647"
     CDS             9302..10381
                     /gene="fbaA"
                     /locus_tag="JQG95_000647"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY5887313.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10572..11432
                     /locus_tag="JQG95_000648"
     CDS             10572..11432
                     /locus_tag="JQG95_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY5887314.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11571..12206
                     /gene="argO"
                     /locus_tag="JQG95_000649"
     CDS             11571..12206
                     /gene="argO"
                     /locus_tag="JQG95_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY5887315.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12299..13039
                     /locus_tag="JQG95_000650"
     CDS             12299..13039
                     /locus_tag="JQG95_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY5887316.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13206..14156
                     /locus_tag="JQG95_000651"
     CDS             13206..14156
                     /locus_tag="JQG95_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5887317.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="JQG95_000652"
     CDS             complement(14098..15576)
                     /gene="scpC"
                     /locus_tag="JQG95_000652"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY5887318.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="JQG95_000653"
     CDS             complement(15600..16376)
                     /gene="scpB"
                     /locus_tag="JQG95_000653"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY5887319.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEDFTLQMAHHISEKAPLAIAVIKEELRVLGEAHT
                     MNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="JQG95_000654"
     CDS             complement(16387..17382)
                     /gene="meaB"
                     /locus_tag="JQG95_000654"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY5887320.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYHQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="JQG95_000655"
     CDS             complement(17375..19519)
                     /gene="scpA"
                     /locus_tag="JQG95_000655"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY5887321.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTIADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19723..20616)
                     /locus_tag="JQG95_000656"
     CDS             complement(19723..20616)
                     /locus_tag="JQG95_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY5887322.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20999..21658
                     /gene="rpiA"
                     /locus_tag="JQG95_000657"
     CDS             20999..21658
                     /gene="rpiA"
                     /locus_tag="JQG95_000657"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY5887323.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21914..23146
                     /locus_tag="JQG95_000658"
     CDS             21914..23146
                     /locus_tag="JQG95_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY5887324.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23375..23434
                     /gene="ibsC"
                     /locus_tag="JQG95_000659"
     CDS             23375..23434
                     /gene="ibsC"
                     /locus_tag="JQG95_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY5887325.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23767..23826
                     /locus_tag="JQG95_000660"
     CDS             23767..23826
                     /locus_tag="JQG95_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY5887326.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23927..24529)
                     /locus_tag="JQG95_000661"
     CDS             complement(23927..24529)
                     /locus_tag="JQG95_000661"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY5887327.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24550..24733)
                     /gene="ssrS"
                     /locus_tag="JQG95_000662"
     ncRNA           complement(24550..24733)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JQG95_000662"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="JQG95_000663"
     CDS             complement(24775..25104)
                     /gene="zapA"
                     /locus_tag="JQG95_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY5887328.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25272..25850
                     /gene="ygfB"
                     /locus_tag="JQG95_000664"
     CDS             25272..25850
                     /gene="ygfB"
                     /locus_tag="JQG95_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY5887329.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25876..27201
                     /gene="pepP"
                     /locus_tag="JQG95_000665"
     CDS             25876..27201
                     /gene="pepP"
                     /locus_tag="JQG95_000665"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY5887330.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27198..28376
                     /gene="ubiH"
                     /locus_tag="JQG95_000666"
                     /gene_synonym="visB"
     CDS             27198..28376
                     /gene="ubiH"
                     /locus_tag="JQG95_000666"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY5887331.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28399..29601
                     /gene="ubiI"
                     /locus_tag="JQG95_000667"
                     /gene_synonym="visC"
     CDS             28399..29601
                     /gene="ubiI"
                     /locus_tag="JQG95_000667"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY5887332.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29675..29758)
                     /locus_tag="JQG95_000668"
     CDS             complement(29675..29758)
                     /locus_tag="JQG95_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887333.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30049..31143
                     /gene="gcvT"
                     /locus_tag="JQG95_000669"
     CDS             30049..31143
                     /gene="gcvT"
                     /locus_tag="JQG95_000669"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY5887334.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKRGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31167..31556
                     /gene="gcvH"
                     /locus_tag="JQG95_000670"
     CDS             31167..31556
                     /gene="gcvH"
                     /locus_tag="JQG95_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY5887335.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31675..34548
                     /gene="gcvP"
                     /locus_tag="JQG95_000671"
     CDS             31675..34548
                     /gene="gcvP"
                     /locus_tag="JQG95_000671"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY5887336.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34815..35557
                     /locus_tag="JQG95_000672"
                     /pseudo
     CDS             34815..35557
                     /locus_tag="JQG95_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(35614..37047)
                     /locus_tag="JQG95_000673"
     CDS             complement(35614..37047)
                     /locus_tag="JQG95_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY5887337.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="JQG95_000674"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="JQG95_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="JQG95_000675"
     CDS             37566..38225
                     /locus_tag="JQG95_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY5887338.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="JQG95_000676"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="JQG95_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY5887339.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSVSRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="JQG95_000677"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="JQG95_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY5887340.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="JQG95_000678"
     CDS             39891..40298
                     /locus_tag="JQG95_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887341.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="JQG95_000679"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="JQG95_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY5887342.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="JQG95_000680"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="JQG95_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY5887343.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="JQG95_000681"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="JQG95_000681"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY5887344.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="JQG95_000682"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="JQG95_000682"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY5887345.1"
                     /translation="MKQQIQLRRREVYETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="JQG95_000683"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="JQG95_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY5887346.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="JQG95_000684"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="JQG95_000684"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY5887347.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="JQG95_000685"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="JQG95_000685"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY5887348.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="JQG95_000686"
     CDS             47703..47774
                     /locus_tag="JQG95_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887349.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="JQG95_000687"
     CDS             complement(47771..47896)
                     /locus_tag="JQG95_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887350.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
BASE COUNT        11472 a        12014 c        12962 g        11497 t
ORIGIN      
        1 gaatttgaac tgccagaaat taccgataat gaactgttag tgagtgtaat ttctgacagc
       61 gtctgtttat cgacctggaa agcggcgtta ctcggtagtg aacataaacg cgtacccgac
      121 gatttagaaa atcatccggt cattaccggg catgaatgtg ccggggttat tgtcgaagtg
      181 ggtaaaaatc tcactggcaa atataaaaaa ggccagcgtt ttgtattgca accggcgatg
      241 gggttaccaa gcggatattc agcgggctac agctacgaat attttggcgg caatgccact
      301 tatatgatta ttcccgaaat cgccattaat ttgggctgcg tattaccgta tcacggctct
      361 tattttgctg cggcgtcgct ggcagagcct atgtgctgca ttattggtgc ttatcatgcc
      421 aattatcaca ccacgcaata tgtttatgag catcgcatgg gcgtcaaacc tggcggcaat
      481 attgcactgc tggcgtgtgc gggtccgatg ggcattggcg ctatcgatta cgccattaac
      541 ggtggcatac aaccgtcacg ggtggtggtg gtcgatatcg acgacaaacg cctggcgcag
      601 gtgcagaagc tgctgccggt ggatctggcg gccagtaaag gcattgagct agtgtatgtg
      661 aataccaaag ggatgagcga tcctatccag atgctgcggg cgctgacagg agatgtcggg
      721 ttcgatgaca tttttgttta tgcggcggtg cctgctgtcg ttgagatggc tgacgaatta
      781 ctggcggaag atggctgtct gaacttcttt gccgggctga cggataaaaa cttcaaagtg
      841 ccgtttaatt tctacaacgt ccattacaac agcacgcacg tagtcggtac atccggcggt
      901 tcaacggacg acatgaaaga ggcgattgcc cttagcgcca caggacagtt acagccgtcg
      961 tttatggtga cccatattgg tggcctggat gcggtgccag aaaccgtgct caatctgccg
     1021 gatatccctg gcggtaaaaa actcatttat aacggcgtga ccatgccgct cactgccatt
     1081 gccgattttg ccgaaaaagg caaaaccgat ccgctgttta aagagttggc gcggctggtt
     1141 gaggaaacgc acggcatctg gaatgaacag gccgagaaat atctgctggc acaatttggc
     1201 gttgatatcg gggaggccgc gcaatgatgt ccctggcgtg gccattattt cgcgttacgg
     1261 aacaggcagc gttggctgcc tggccgcaca ccggatgtgg cgacaaaaat aaaattgatg
     1321 gcctggcggt caccgcgatg cgccaggcat taaacgacgt cgcttttcgt ggacgagtgg
     1381 ttatcggcga aggagaaatt gaccatgcgc cgatgctgtg gattggcgaa gaggtgggca
     1441 aaggcgatgg gccagaagtc gatatcgcgg ttgatcccat tgaaggcacg cggatggtgg
     1501 cgatggggca gagcaatgcg ctggcggtca tggctttcgc tcctcgagat agcctgttac
     1561 atgcgcccga tatgtatatg aaaaagctgg ttgttaatcg actggctgcc ggggcgattg
     1621 atctgtcttt gcctctggcg gacaacttgc gtaacgtggc gcgggcgtta ggtaaaccgc
     1681 tggacaagct gcgcatggtt acgctggata aaccgcgtct gagtgccgcg attgaagaag
     1741 caacacagct gggcgtgaag gtttttgccc tgccggatgg cgatgtcgcc gccagcgtgc
     1801 tgacctgctg gcaggataat ccttacgatg tgatgtacac catcggcggt gcgccagaag
     1861 gcgtgatttc tgcctgtgcc gttaaagcgt taggcgggga tatgcaggcc gagctgattg
     1921 acttttgtca ggctaaaggg gattacacgg aaaatcggca gattgcagag caggagcgca
     1981 agcgttgtaa ggcaatgggc gttgacgtta accgtgtgta ctcgctcgat gaactggtga
     2041 gggggaacga tatcctcttt agcgctacgg gcgtgacggg cggcgagttg gtgaacggta
     2101 tccaacagac ggcgaatggg gtgcggacgc agacattact gatcggcggc gcggaccaaa
     2161 cgtgtaatat aatagactcc ctgcattgat ggtgatctat cggaagacca atggcgacgc
     2221 tgacagaaga tgatgtgctt gagcaactgg atgcacagga caatttattt tcatttatga
     2281 aaactgcgca ttctattttg ctccagggga tacgccagtt tctgccgtcg ctgtttgtcg
     2341 ataacgatga agagatcgtc gaatatgcag tgaagccgtt actcgcccaa agcggcccgc
     2401 ttgacgatat tgatgttgcg ctgcgtttga tttatgcgct ggggaaaatg gataaatggc
     2461 tgtatgccga tatcacgcac ttttcccagt actggcatta cctgaacgaa caggatgaaa
     2521 cgcccggatt tgccgatgat ataacctggg attttatcag caatgtcaac agtataactc
     2581 gcaatgcgac gctctacgat gcgttaaaag cgatgaagtt cgccgatttc gccgtctggt
     2641 cagaggcgcg ttttagcgga atggtcaaaa cggcgctgac gctggcagta acgaccacct
     2701 taaaggaatt aacgccgtga aaattgaatt aacggtgaat gggctgaacg tgcaagcgca
     2761 gtaccatgat gaagagatag aacgcgtgca taaactgttg ctgcgtatgc tggcggcctt
     2821 acagacggta aatccgcagc ggcgtacggt ggtttttctt tgcgctccgc cggggacggg
     2881 taaatctacc ctaaccacct tctgggaata tctcgcgcaa caagacccgg agctgcccgc
     2941 tattcaggcg ctcccgatgg atggttttca ccattacaat agctggctgg acgcgcatca
     3001 attgcgcccc ttcaaaggcg caccagagac atttgacgtt gcgaaactgg cggaaaatct
     3061 gcgccaggtg gtggaagggg attgtacgtg gccgcagtac gatcgacaaa agcatgatcc
     3121 tgttgaagat gcgttgcacg ttaccgcacc actcgtcatc gtcgaaggaa actggttgtt
     3181 gctggacgac gagaagtggt gccagcttgc gcaattttgt gatttctcga tctttatcaa
     3241 cgcgccagct gctgcgttgc gggagcgtct ggtggggcgc aagctggcgg gaggggtatc
     3301 gctggcagat gcagaagcct tttatgaccg taccgacggg ccgaatgttc gccgggtgct
     3361 ggaagagagt ctgcctgcaa atttgacctt aatgatgacc gctacgggtg aatatcgtct
     3421 ggtggattaa cgttcctgat tacaccatct tccagccgca ccacacgaga gaaatgacgc
     3481 cgggtaaatg cggcgtcatg actgattgct accacgctcg tgcctcgctg gcggcacttc
     3541 tccagccagc tttcaaacac gctcaaccaa ttttcgtcaa aatcccgact cggctcgtcc
     3601 agcagtaata gtggcggaga gagtgcttcc aggcaggcga cggcgaccat ccgtcgctgc
     3661 gcactatgca gatccaacgg gtgcgagttg gcagtgtctg ctaactgaca acattgcagg
     3721 gcagcatggg tacgttgagt gatttcatcc gccgggcatt tttgcagttt caaaccaaat
     3781 gcgatctcgt cagcgacggt gctgtgaaaa agttggtttt ccgcttcctg aaacagaaca
     3841 gtacgccaac ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca
     3901 gcataaccgt accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt
     3961 tccctgcgcc attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa
     4021 tgtcgcaaag acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta
     4081 acatagtggc tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa
     4141 aggtgtgcga tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg
     4201 taagcgctgc aacagcattt cgctggcctg cggcgtcagg cggctaaagg cttcatcaag
     4261 aattaagagc tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc
     4321 accggagagt gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc
     4381 ggcatcaatc cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc
     4441 aacttcttct tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac
     4501 gagctggatg gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag
     4561 cgtccccgtg cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg
     4621 tgccagcgta cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac
     4681 atagtgaaag tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg
     4741 ttctaacgta accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa
     4801 cgcgctacgc gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg
     4861 atccgaaatg cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg
     4921 tgtgttagcg gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc
     4981 agcggcacgc cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc
     5041 tcaacgacca ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca
     5101 aacagagcgc gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa
     5161 acaatcgcca gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt
     5221 ggcgtgccgc ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc
     5281 agcgagaaca tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata
     5341 aggcagaaaa aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc
     5401 gtggtgcagg ccgccaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg
     5461 gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag
     5521 acaatcaccg ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc
     5581 gactccacca ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta
     5641 ccggtgacac cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc
     5701 gtaataatca cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc
     5761 agccaaccgg agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg
     5821 tcggtcagca atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga
     5881 ccaagcagta ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc
     5941 atgctggcca gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt
     6001 gcctgactgg aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac
     6061 gggggaaata tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag
     6121 ttcctcaagg ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat
     6181 atgaccgcca agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact
     6241 ctgttccagc gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc
     6301 ttcttgcccg gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta
     6361 ggcggcatga agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc
     6421 ggggaggagg cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat
     6481 cattggagta ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata
     6541 ttctgatata agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt
     6601 gattctgcaa agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga
     6661 taattgcttt ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct
     6721 gaagcgtttc agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg
     6781 taaggtttcc ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac
     6841 cttgcaggag atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta
     6901 atgtggttcg tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca
     6961 acgaactggc ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc
     7021 gttttgcatg ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc
     7081 gcgtattgca tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag
     7141 tcctcgactg caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg
     7201 gggccaaaaa agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt
     7261 acggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta
     7321 ccacgaattg cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg
     7381 gcactgtgac cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc
     7441 ctgacctgcg tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg
     7501 ccgctggtat cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac
     7561 gtgtgccaac cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa
     7621 aagccaatga agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag
     7681 ttgactatac ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca
     7741 ttgtcgatgg cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt
     7801 gcgataacga atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg
     7861 ttgctttcag gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc
     7921 accatgtctg taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt
     7981 gcggatctga acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct
     8041 tctctgccga ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac
     8101 ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac
     8161 tacctgaaag acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt
     8221 gacgttgctg aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag
     8281 aaagacgacg aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac
     8341 gcattcggta ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac
     8401 gttgcgtgcg caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa
     8461 gaacctgctc gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc
     8521 gttctggact ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac
     8581 acctttatcg cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt
     8641 gacgaagcta aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta
     8701 gcaaccgagt tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct
     8761 gacgagcaga tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag
     8821 aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc
     8881 aaaggtactg aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc
     8941 ggcggcgaca ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc
     9001 tccactggcg gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg
     9061 atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt
     9121 cagcgcgctt ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc
     9181 gacaagagag tgaatcccca ggagcttaca taagtaagtg actgggggga gcgaacgcag
     9241 acgcagcaca tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga
     9301 catgtctaag atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa
     9361 agttttccag gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac
     9421 tgactccatc aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca
     9481 gttctccaac ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca
     9541 gggtgctgct atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca
     9601 ttatggtgtt ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat
     9661 cgacggtctg ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc
     9721 ttctcacatg atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa
     9781 atacctggag cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg
     9841 tggtgaagaa gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc
     9901 ggaagacgtt gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc
     9961 tgcgtccttc ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac
    10021 catcctgcgt gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct
    10081 gaacttcgta ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag
    10141 ctacggcgta gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt
    10201 tctgaactac tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg
    10261 cgaagatcag ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac
    10321 ttcgatgatc gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta
    10381 agtcattcct ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca
    10441 aggtgattca gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc
    10501 ttgtcagact gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa
    10561 aggaatattg aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc
    10621 tggtagctaa ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga
    10681 tcatcatcgt tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga
    10741 tctcccgtaa aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta
    10801 ttatcgcctt tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca
    10861 ttgctgtact cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta
    10921 acctggccgc tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg
    10981 acctcggcgg cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta
    11041 ctgcagacgg taaaattatc gttattccga acggtaaaat tattgccgga aatattatta
    11101 acttctcccg cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg
    11161 atatcgatca ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga
    11221 aagatcgcga aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg
    11281 tccgcgtctg gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta
    11341 ttaaacgtga atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact
    11401 ttaagcgggt gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct
    11461 ggcctctctc ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac
    11521 aatcccgcga tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt
    11581 attactttca aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg
    11641 cttttgtgat gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg
    11701 ctatcagcga tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc
    11761 agtcgccgtg gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg
    11821 gttttggcgc ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc
    11881 tgaagcaagg cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc
    11941 atgtttacct ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac
    12001 caaaacgttg gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg
    12061 ctattctcgc agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca
    12121 atctggtagt gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg
    12181 ctcatgcaca agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct
    12241 aagcttgcct ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt
    12301 gaagttcaaa gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc
    12361 taacgaattg ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt
    12421 gccagacatt gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc
    12481 caagaaacag gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat
    12541 cgcgaaaaaa gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga
    12601 tggtaaaagt atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca
    12661 gttagacaaa ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg
    12721 ttctgtgtcg ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc
    12781 gattgataac gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg
    12841 gccggtatat agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat
    12901 gatgaaagcc gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca
    12961 gtttgatgat caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa
    13021 aacacctgca gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc
    13081 cgatattgcc tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    13141 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag
    13201 gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca
    13261 ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca
    13321 ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca
    13381 gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt
    13441 ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca
    13501 tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg
    13561 aaggcgtgac tgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg
    13621 accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg
    13681 aaaacctgac tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt
    13741 atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc
    13801 aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc
    13861 ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac
    13921 attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat
    13981 attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc
    14041 atcgttatac cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta
    14101 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    14161 gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat
    14221 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    14281 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg
    14341 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa
    14401 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    14461 gaccccggct acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt
    14521 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    14581 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    14641 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    14701 caccgattcc tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc
    14761 cagacgtgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    14821 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    14881 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    14941 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    15001 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc
    15061 cagttctgca acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg
    15121 cagcagccag gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc
    15181 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    15241 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    15301 acgtaaactg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    15361 tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt
    15421 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga
    15481 acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    15541 ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    15601 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    15661 tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    15721 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    15781 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccacttc cacaacatgg
    15841 ttgaggatgc cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc
    15901 tctttgacaa tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac
    15961 gggacgccga ggtttacagg cgtcattgag aaggttgagg tactggcggc gatgatcaga
    16021 tcggaactca tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg
    16081 atcggtttcg ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag
    16141 agcggatcgc gaccgccaga cggcagttcg tgaatatcgt gacctgcgga gaagactttg
    16201 gatccacttg gcgcccgcaa aatgatacag cgaatttccg gccggttgag atcgcttaac
    16261 gcctgcataa gatcatcaat aaagacttta cttaaggcat taagttttcg tccatagtta
    16321 aactcaatga ccgccacttt gttgatagtg gcaacgttca catactgata agacataaaa
    16381 attcctttaa tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg
    16441 cggtgagagc gtattgtttt tgaccgctaa aagcgtctgg tggtaatagc gatcgaaatc
    16501 ttcattcgcg aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac
    16561 cgattgttgt tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtc
    16621 gatgatggcg tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagacc
    16681 ccgtggctgc cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg
    16741 ccgggcaatg gcgacattgg tatggttatc gccatcgtct ttgttgataa cgagcagatc
    16801 agccacttcc atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg
    16861 caacgagata aaacagtcca ccatgcgggc gacttccgtt tccgactgcg cgacgccaac
    16921 cgtttcgaca atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgtgc
    16981 tcgctgactg gcaccgccca gatgaccgga ggagggtacc gggcgaataa acgccgcctc
    17041 ggcacgcgcc aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg
    17101 gctgctggga tcgaccgcaa taacagcaac ctttaatccc tcacgaatca acaacatgcc
    17161 aaaggcttca agaaaggtac ttttccccgc gccgggtgtg ccggtaacgc ccagtcgcag
    17221 ggcgttacca cagaacggca taatagcgtc aagcagctgt gtacttagcg cctgatgacg
    17281 tgggtgacgg ctttccacca gcgtcatggc ttgggcgagc gtggcacgct caccctgacg
    17341 taagcggcga atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat
    17401 tcagtacgtc gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc
    17461 cgcgctcttg caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga
    17521 tatcttcgcg tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac
    17581 cggcagccag tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc aggcgggcga
    17641 tctcttcagg tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg
    17701 cgctggcgat cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa
    17761 tgcgcgggcg acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga
    17821 actcggaggc cgatttctca gactgatgat agctttgcgc aatcacgccg gtaacacact
    17881 ggcttggcac cagataacgg tcgaaagcga cttccagcgc atcggaaatt tcacccaggg
    17941 tggcgcgaac gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg
    18001 cgtgagtcag ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc
    18061 gttccagcga agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg
    18121 tttcgtcttc gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt
    18181 cgatcagcga ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt
    18241 cgatcgcttt cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt
    18301 tgacgatttg atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgt
    18361 ggcagagttc tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga
    18421 aatcggtagg caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc
    18481 ccagcgtcgc agccagtgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt
    18541 cagtcaggct ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcggatcct
    18601 gtgcgccaaa tccactgacc gcttcgctcc ataaataacg tgccgcacgc aacatggcga
    18661 cgttcataaa cagatccatg ccgataccga agaataacga caggcgagga gcgaagtcat
    18721 caattttcag tccggcagag attgctgctt tgatgtactc aatcccatcg gcgatcgtaa
    18781 atgctacctg ctgcacgcag ttggcacccg cttcccccat gtggtaaccg ctgatactga
    18841 tggtattaaa tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca
    18901 ttgacggttt tggtgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt
    18961 tttgaatggt gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat
    19021 aaaacgccag tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca
    19081 gcgggatctg gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt
    19141 tgccgacgtc acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt
    19201 caaacgcaac ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg
    19261 actcttttgc tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca
    19321 tagtggcacg cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca
    19381 gattatcgag atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct
    19441 gaaccagcga gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc
    19501 actcctgctc gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg
    19561 cttacggtga gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt
    19621 tttttgccgc ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg
    19681 aggacaacgc ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac
    19741 tttgtgacca taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc
    19801 aaagcggtgc cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc
    19861 accgctggcc agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg
    19921 gcgagcaagt tgtacgaacg cttctgaaga attaacgata tggcagggta cgctgccagg
    19981 aggaagatcg aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc
    20041 gaccactggc gctttcaata atgccgaacg cgttacgccg ttaggaaaat atttttcggc
    20101 aaacggtttt gagctgacga acagatagtc gagcgcaccg agtttatcga caagacaact
    20161 cggcagcgcc tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc
    20221 ctgagtacgg gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac
    20281 aggagccagt gccggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag
    20341 cagcagcgga gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac
    20401 ctggcgcagc agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg
    20461 caccaacagc ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga
    20521 ttgtgtaatg cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc
    20581 cagtgcctgt aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat
    20641 gctttttcct gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg
    20701 ggtaacattt gtatcaatgg ttaaacgcgc agcttaaaag atcagggatt tgcgctattg
    20761 catttcgctt agcgacttcg cgccctggtt atgttgaatg gcgtggcgtt attgcctctg
    20821 ttcgcaccgt ggagcggcat acccagaccg ttgtatgctt tatttgcggg gcgaaagggg
    20881 atgcccgcca ttgcgcgata ttttatggat gagttaacca cccagcttgc caacggggtc
    20941 tgaatcgctt tttttgtata taatgcgtgt gaaatttcat accacaggcg aaacgatcat
    21001 gacgcaggat gaattgaaaa aagcagtagg atgggcggca cttcagtatg ttcagcccgg
    21061 taccattgtt ggtgtaggta caggttccac cgccgcacac tttattgacg cgctcggcac
    21121 gatgaaaggc cagattgaag gggccgtttc cagttcagat gcttccactg aaaaactgaa
    21181 aagcctcggc attcacgttt ttgatctcaa cgaagtcgac agccttggca tctacgttga
    21241 tggcgcagat gaaatcaacg gccacatgca aatgatcaaa ggcggcggcg cggcgctgac
    21301 ccgtgaaaaa atcattgctt cggttgcgga aaaatttatc tgtattgccg acgcctccaa
    21361 gcaggttgat attctgggta aattcccgct gccagtagaa gttatcccga tggcacgtag
    21421 tgcagtggcg cgccagctgg tgaaactggg cggtcgtccg gaataccgtc agggcgtggt
    21481 gaccgataat ggcaacgtga tcctcgacgt ccacggcatg gaaatccttg acccgatagc
    21541 gatggaaaac gccataaatg cgattcctgg cgtggtgact gttggcttgt ttgctaaccg
    21601 tggcgcggac gttgcgctga ttggcacacc tgacggtgtc aaaaccattg tgaaatgatc
    21661 tgacggggga acctcccccg ttaaaaaaat tctcttcatt aaatttggtg acatgtgtca
    21721 cgcttttacc aggcaattgt cgattgctct aaataaatcc tctaaaccag catattcatc
    21781 caagaattac ctttgcgtga tatttcctca acatcgcgac gcaaacgttc atattgccgc
    21841 aatattattt tttgatatgt tgaaaggcgg atgcaaatcc gcacacaaca tttcaaaaga
    21901 caggattggg taaatggcaa aggtatcgct ggagaaagac aagattaagt ttctgctggt
    21961 agaaggcgtg caccaaaagg cgctggaaag ccttcgtgca gctggttaca ccaacatcga
    22021 atttcacaaa ggcgcgctgg atgatgaaca attaaaagaa tccatccgcg atgcccactt
    22081 catcggcctg cgatcccgta cccatctgac tgaagacgtg atcaacgccg cagaaaaact
    22141 ggtcgctatt ggctgtttct gtatcggaac aaaccaggtt gatctggatg cggcggcaaa
    22201 gcgcgggatc ccggtattta acgcaccgtt ctcaaatacg cgctctgttg cggagctggt
    22261 gattggcgaa ctgctgctgc tattgcgcgg cgtgccggaa gccaatgcta aagcgcaccg
    22321 tggcgtgtgg aacaaactgg cggcgggttc ttttgaagcg cgcggcaaaa agctgggtat
    22381 catcggctac ggtcatattg gtacgcaatt gggcattctg gctgaatcgc tgggaatgta
    22441 tgtttacttt tatgatattg aaaacaaact gccgctgggc aacgccactc aggtacagca
    22501 tctttctgac ctgctgaata tgagcgatgt ggtgagtctg catgtaccag agaatccgtc
    22561 caccaaaaat atgatgggcg cgaaagagat ttcgctaatg aagcccggct cgctgctgat
    22621 taatgcctcg cgcggtactg tggtggatat tccggcgctg tgtgatgcgc tggcgagcaa
    22681 acatctggcg ggggcggcaa tcgacgtatt cccgacggaa ccggcgacca atagcgatcc
    22741 atttacctct ccactgtgtg aattcgacaa cgtccttctg acgccacaca ttggcggttc
    22801 gactcaggaa gcgcaggaga atatcggctt ggaagttgcg ggtaaattga tcaagtattc
    22861 tgacaatggc tcaacgctct ctgcggtgaa cttcccggaa gtctcgctgc cactgcatgg
    22921 tggtcgtcgt ctgatgcaca tccacgaaaa ccgtccgggc gtgctaactg cgctgaacaa
    22981 aatcttcgcc gagcagggcg tcaacatcgc cgcgcaatat ctacaaactt ccgcccagat
    23041 gggttatgta gttattgata ttgaagctga cgaagacgtt gccgaaaaag cgctgcaggc
    23101 aatgaaagct attccgggta ccattcgcgc ccgtctgctg tactaattcc ccttctctga
    23161 aaattaacgg gcaggtcact gacttgcccg tttttttatc ccttctccac accgttgccg
    23221 cgttgcgcat tctactttgc gagtctgctc cagaaaataa atcaaagtca gatcaatgcg
    23281 ttgtgtttat ttaatgggga tttacgatgg cgggacagca tggggctgta acggtaaagc
    23341 cctcaccgaa gcgagggctt aaaggagaag ggttatgatg cgacttgtca tcatactgat
    23401 tgtactgtta ctgataagtt tcggagctta ttaacagtca gtctcaaggg aggagtcatc
    23461 ctcccttacc cttactcact aaattaggtc aaagaatcaa cgatgtcaat cagggcgatg
    23521 cggggtgcat cgccctgaac cgaacttttt gatagccact tcatcgccca cgtttactgc
    23581 cgtaatagat cctttttccc aaaaattatc tctgttttgc atggggtttt tcgagcacgc
    23641 tcgaagaaaa gatttaaagg tagatcaatg cgttgtggtt atttaaagag agcttacgat
    23701 agctgggcag catggggctg taacggtaaa gccctcaacg aagcgagggc ttgaaggaga
    23761 agggttatga tgcgacttgc catcatactg attgtactgt tactcataag tttcccagct
    23821 tattaacagt cagtcacagg ggaggagcaa tcctccctta cccttactca ctaaattagg
    23881 ttaaagaatc aacgatgtca atcagggcga tgcaggtgta tcgcccttac cactcccaga
    23941 ctttcgacgg tgtaaccacc gcaggaagag ggatatccca ctcttcaacg gggagttttt
    24001 ccaccaactg acaatcatgc gcataaccca ccggttgcgt tttatagtgc tgccagtttt
    24061 gtaaggtccg atcataaaaa ccaccgccca ttcccaggcg ctgaccgtac tcatcaaagg
    24121 cgaccagcgg tgtgatcagc acgtctaatc gggaaagggg tagcacgtca cgcacatcca
    24181 gttttggctc atggatcttc aacctgttca tcaccagttc gctttgcgga tggtaattca
    24241 ggaacagcaa attaccggca ctaaagggat gcaaaactgg aagatatacg cgcttaccgg
    24301 cgcgccagag ttgttctatt agcggctggg tgtcgagttc accatcaaaa gagaggaata
    24361 cagcgaccgt atgcgccatc accaccgggg gataagtcat catccgggta gcggcttgtt
    24421 gacccatttc cagctgttgt tccggcgtta acgcacgacg acgttgccga atcattttgc
    24481 ggatttcttg tcgggataat gtcagtggga gctctggtag ttgcgtcatg gctggtgcca
    24541 gataagaagg gaatctccga gatgccgccg caggctgtaa cccttgaacc cttggttcaa
    24601 ggtgaatgtg tcgtcgcagt tttaaggctt ctcggacgaa ccgagcatgc tcaccaaccg
    24661 cggagcgcca cattcttgtg gtatgaaata tcggctcagg ggactggccc gcttgcgaac
    24721 atctcagaga aattttgtct tcacggttac tctaccacag taaaccgaaa agtgtcattc
    24781 aaagttttgg ttagtttttt cggtgatgcg accttgttca agtaacgctt gttctatggt
    24841 ctgctgcagc atccgaatac gctgttccat acttgccgcg tagtcacgag tctttgcttt
    24901 ttcttgcgct aactcatagc tgatattcaa tgcggcaatg aagaccaact gttcagtatt
    24961 tgtgactcta gtgcgttctt tcagatcttg caaccgttgg ttcagatcgt ccgctgcctg
    25021 attcaacgca tccctttggt caggcgggca gttcacacgc agtgaacggc caaaaatttg
    25081 gatatcgacg ggttgtgcag acatgccacc ttcctgctga ttgactgcgc tgccttcgtc
    25141 tttagaccca tggtctgcga aggggcgaca ctatagctac cctgatgaga agagacaagc
    25201 ccttttctgg tccaccaggg cccaaagtgg tagcatatca tgaatattcc tccctttgac
    25261 gacgaatgct tatgtctata cagaacgaaa tgcctggtta caacgaaatg aaccagtatc
    25321 tgaaccaaca agggacgggt ctgacccctg ccgagatgca tggtttaatc agtgggatga
    25381 tatgtggcgg taacgatgac agctcatggc tgccgctact tcacgacctg acgaacgaag
    25441 gcatggcttt cggtcatgag ctggcacagg cactgcgtaa aatgcactct gccaccagcg
    25501 atgccctgca ggatgacggc ttcctttttc agctttatct gcctgatggc gatgatgtca
    25561 gcgttttcga tcgggctgat gcactggcag gttgggtcaa tcacttcctg cttggtcttg
    25621 gcgttacgca accgaagctg gacaaagtga ccggcgaaac cggtgaagcc atcgacgatc
    25681 tgcgtaacat cgcgcagctg ggctacgacg aagacgaaga tcaggaagag cttgaaatgt
    25741 cgcttgaaga gatcatcgaa tacgttcgtg ttgccgcgct gttatgccac gacaccttta
    25801 ctcatccgca accgaccgcg ccagaagtac aaaaaccgac tctacactaa aaacaaaaaa
    25861 cgtaaggaga gtgttatgag tgagatatcc cggcaagagt ttcagcgtcg ccgtcaggcc
    25921 ctggtggagc aaatgcaacc cggcagcgcc gcgctgattt ttgctgcacc agaagtaaca
    25981 cgtagcgccg acagcgaata cccctatcgt cagaacagtg acttctggta cttcaccggc
    26041 tttaacgaac cggaagcggt gctggtgctg attaaaagcg atgacactca taaccacagc
    26101 gttctgttta accgcgttcg cgacctgacg gcggagatct ggtttggccg tcgcttaggc
    26161 caggatgccg cgccagagaa actgggcgtt gaccgcgcac tggcattcag cgaaatcaat
    26221 cagcaacttt atcaactact taacggcctg gatgtggttt accatgccca gggcgaatat
    26281 gcatatgctg atgaaatcgt gaacagtgcg ctggaaaaac tgcgtaaagg ttcgcggcaa
    26341 aatctcaccg caccggcaac gatgatcgac tggcgtcctg ttgttcatga aatgcgcctg
    26401 ttcaaatcgc cagaagagat tgccgtactc cgccgcgcgg gagaaatcac cgccatggca
    26461 catacacggg cgatggaaaa atgccgtccg ggaatgttcg agtaccatct ggaaggcgaa
    26521 attcaccacg aatttaaccg ccacggtgcg cgctatcctt cctataacac cattgtaggc
    26581 agcggtgaaa acggctgcat tctgcactac accgaaaacg agagtgaact gcgcgacggt
    26641 gacctggtgt tgattgacgc gggttgtgaa tacaaaggtt acgcgggcga tattacccgc
    26701 accttcccgg tcaacggcaa attcacccag gcccagcgtg aaatctacga cattgtgctg
    26761 gagtctctcg aaaccagcct gcgcctgtat cgtccgggaa cttccattct ggaagtcact
    26821 ggtgaagtgg tgcgcatcat ggttagcggc ctggtaaaac tcggcatcct gaaaggtaat
    26881 gttgatgaac tgatcgctca gaacgcccat cgtcctttct ttatgcatgg ccttagccac
    26941 tggttaggac tggatgtcca tgacgttggc gtttatggtc aggatcgctc gcgcattctg
    27001 gaaccgggga tggtactgac cgtagagcct ggtctgtata tcgcgccgga tgcggatgtg
    27061 ccagaacaat atcgcggtat cggcattcgc attgaagacg acattgtgat taccgaaacc
    27121 ggcaacgaaa acctcaccgc cagcgtggtg aaaaagccgg aagaaatcga agcgttgatg
    27181 gctgctgcga gaaagcaatg agcgtaatca tcgtcggtgg cggcatggcg ggcgcgacgc
    27241 tggcgctggc tatttcccgg ttaagtcacg gggcgctgcc ggtacatttg attgaagcga
    27301 ctgcgccaga gtcacatgct catccgggct ttgatggacg agcgatagcg ctggcggcgg
    27361 gtacctgtca gcaactggcg cgcatcggcg tctggcaatc tctggctgat tgcgcaaccg
    27421 ccatcactac cgtgcatgtc agcgatcgtg gtcatgctgg attcgtcacc ctcgccgcag
    27481 aagattacca actggcggcg ctggggcagg ttgtcgaatt gcataatgtc gggcaacggc
    27541 tgtttgcatt gctgcgaaaa gcacctggcg taatgctgca ttgccccgat cgcgtagcta
    27601 acgttgcccg tactcagagt catgttgaag tgacgctgga gagcggcgag atgctgacgg
    27661 gccgcgtgct gatagccgct gatggcaccc attcagcgtt agccaccgcg tgcggcgttg
    27721 attggcagca ggagccttac gaacaactgg cagtgattgc caacgttgct acttctgttg
    27781 cgcacgaagg ccgcgctttt gaacgcttca cgcaacatgg cccgctggcg atgttgccga
    27841 tgtctgacgg acgctgttcg ctggtctggt gtcatccact ggaacggcgc gaagaggtgc
    27901 tgtcgtggag cgacgagaag ttttgccgtg aactccagtc tgcctttggc tggcgactgg
    27961 ggcaaattac ccacgccggt aaacgcagtg cttatccgct ggcattaacc cgcgccgcca
    28021 gaccgattac ccatcgcact gtgctggtgg gcaatgcggc gcaaactctg cacccgattg
    28081 ccgggcaagg gtttaacctc ggtatgcgag atgtcatgag ccttgcggaa accctgactc
    28141 aggcgcatga gcgcggagaa gacatcggtg attacggcat attgtgccgc tatcagcagc
    28201 gtcgacagag cgatcgcgaa gcaaccatcg gcgtcacgga cagcctggta catctttttg
    28261 ccaaccgttg ggcaccgctg gttgtcgggc gcaacatcgg actgatgacg atggaattat
    28321 tcaccccggc acgcgatgtg ctggcgcagc gcaccctcgg ttgggtggcg cgttgaaggc
    28381 ggttttaagg aataaacaat gcaaagtgtt gatgtagcca ttgttggcgg cggcatggtg
    28441 gggctggcgg ttgcctgcgg cttacagggg agcggcttac gcgttgccgt actggagcag
    28501 cgcgtaccgg aacctctggc ggtagatgca ctaccacaac tgcgcgtttc ggctatcaat
    28561 gccgccagcg aaaaattact cacccgtctt ggcgtctggc aggacattct cgctcgtagg
    28621 gccagttgtt atcacggcat ggaagtgtgg gacaaagaca gttttggtca catctcgttt
    28681 gacgatcaaa gtatgggcta tagccatctt gggcacatcg ttgaaaactc agtgattcac
    28741 tacgcgctgt ggaacaaagc gcagcagtcg tcagatatta ctctgttggc ccccacagaa
    28801 ttacagcagg tcgcctgggg agaaaatgaa accttcctga cgctgaaaga tggcagcatg
    28861 ttaacggcgc gtctggtgat tggcgcggac ggtgctaatt cctggttgcg caacaaagca
    28921 gatattccgc tgactttctg ggattatcag catcacgcgc tggtagcgac cattcgcacg
    28981 gaagaaccgc atgatgcggt ggcgcggcag gttttccatg gcgaaggcat tctggccttt
    29041 ttaccgctta gcgatccgca tctttgctcg attgtctggt cactgtcgcc agaggaagcg
    29101 cagcggatgc agcaggcaag tgaagacgaa tttaatcgcg cgttaaatat cgcttttgat
    29161 aatcgccttg gcttatgcaa ggttgagagc gcgcgtcagg tgttcccgct gacggggcgt
    29221 tatgcgcgcc agtttgccgc acaccgtctg gcgctggtgg gcgacgccgc gcataccatt
    29281 cacccactgg cggggcaggg cgttaatctc ggatttatgg acgccgcaga gctgattgct
    29341 gaactgaaac ggttgcatcg tcagggtaaa gacatcgggc agtacattta cctgcgccgc
    29401 tatgaacgta gccgcaagca cagcgcggcg ctgatgctgg ctggtatgca gggttttcgc
    29461 gatctctttt ccggcgctaa tccggcgaaa aaactgctgc gtgatattgg tttgaaactg
    29521 gccgacacgc ttcctggcgt taagccgcaa cttatccgcc aggcaatggg attaaacgat
    29581 ttgcctgaat ggctgcgtta aaaatttctc ctctgttgtt tatttgatac ccatcacact
    29641 ttcatctccc ggttttttcg ccgggagatt ttcctcattt gaaataaact aatttcacct
    29701 ccgttttcgc attatatttt ctaataccat gattttttga tttagtgttt tttgacattt
    29761 ttttagctct taatattgtc ttattcaaat tgactttctc atcacatcat ctttgtatag
    29821 aaattggtgt attttttggt tttttattct gtcgcgattt ttgcattttt taaccataag
    29881 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
    29941 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt tgtggtgata
    30001 atttcaccat gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag
    30061 actcctttgt acgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg
    30121 atgatgccgc tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc
    30181 ggaatgtttg atgtgtcgca tatgaccatc gtcgatctcc gcggcagccg cacccgggag
    30241 tttctgcgtt atctgctggc gaacgatgtg gcgaagctca ccaaaagagg caaagccctt
    30301 tactcgggga tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt
    30361 actgaagatt tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg
    30421 attacccaac acgctgaacc tttcggcatc gaaattaccg ttcgtgatga cctttccatg
    30481 attgccgtgc aagggccgaa tgctcaggca aaagctgcca cactgtttaa tgatgctcag
    30541 cgtcaggcgg tggaagggat gaaaccgttc tttggcgtgc aggtgggcga tctgtttatt
    30601 gccaccactg gttataccgg tgaagcgggc tatgaaattg cgctgcccaa tgaaaaagcg
    30661 gccgatttct ggcgtgcgct ggtggaagcg ggcgttaagc cctgcggtct gggggcgcgt
    30721 gacacgctgc gtctggaagc gggcatgaat ctttatggtc aggagatgga cgaaactatt
    30781 tctcctttag ccgccaacat gggctggact atcgcctggg aaccgacaga tcgtgacttt
    30841 atcggtcgtg aagccctgga agtgcagcgt gagcatggta cagaaaaact ggttggtctg
    30901 gtgatgaccg aaaaaggcgt gctgcgtaat gaactgccgg tacgctttac cgatgcgcag
    30961 ggcaaccagc atgaaggcat tatcaccagc ggtactttct ccccgacgct gggttacagc
    31021 attgcgctgg cgcgcgtgcc ggaaggtatt ggcgaaacgg cgattgtgca aattcgcaac
    31081 cgtgaaatgc cggttaaagt gacaaaacct gtttttgtgc gtaacggcaa agccgtcgcg
    31141 tgatttactt ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca
    31201 aagaacacga atggctgcgt aaagaagccg acggcactta caccgttggt attaccgaac
    31261 atgctcagga gctgttaggc gatatggtgt ttgttgacct gccggaagtg ggcgcaacgg
    31321 ttagcgcggg cgatgactgc gcggttgccg aatcggtaaa agcggcgtca gacatttatg
    31381 cgccagtaag cggtgaaatc gtggcagtaa acgacgcact gagcgattcc ccggaactgg
    31441 tgaacagcga accgtatgca ggcggctgga tctttaaaat caaagccagc gatgaaagcg
    31501 aactggaatc actgctggat gcgaccgcat acgaagcatt gttagaagat gagtaacggc
    31561 tttattcctc ttctgcggga gaggatcagg gtgaggaaaa tttatgcctc accctcactc
    31621 tcttcgtaag gagagaggtt cacaattcac tgcacgtttc aggaaccatc gctcatgaca
    31681 cagacgttaa gccagcttga aaacagcggc gcttttattg aacgccatat cggaccggac
    31741 gccgcgcaac agcaagaaat gctgaatgcc gttggtgcac aatcgttaaa cgcgctgacc
    31801 ggccagattg tgccgaaaga tattcagctt gcgacaccac cgcaggttgg cgcaccggcg
    31861 accgaatacg ccgcactggc agaactcaag gctattgcca gtcgcaataa acgcttcacg
    31921 tcttacatcg gcatgggtta caccgccgtg cagctaccgc cggttatcct gcgtaacatg
    31981 ctggaaaatc cgggctggta taccgcgtac actccgtatc agcctgaagt ctcccagggc
    32041 cgccttgaag cactgctcaa cttccagcag gtaacgctgg atttgactgg actggatatg
    32101 gcctctgctt ctcttctgga cgaggccacc gctgccgccg aagcaatggc gatggcgaaa
    32161 cgcgtcagca aactgaaaaa tgccaaccgc ttcttcgtgg cttccaatgt gcatccgcaa
    32221 acgctggatg tggtccgtac tcgtgctgaa acctttggtt ttgaagtgat tgtcgatgac
    32281 gcgcaaaaag tgctcgacca tcaggacgtc ttcggcgtgc tgttacagca ggtaggcact
    32341 accggtgaaa ttcacgacta cactgcgctt attagcgaac tgaaatcacg caaaattgtg
    32401 gtcagcgttg ccgccgatat tatggcgctg gtgctgttaa ctgcgccggg taaacagggc
    32461 gcggatattg tttttggttc ggcgcaacgc ttcggcgtgc cgatgggcta cggtggccca
    32521 cacgcggcat tctttgcggc gaaagatgaa tacaaacgct caatgccggg ccgtattatc
    32581 ggtgtatcga aagatgcagc tggcaatacc gcgctgcgca tggcgatgca gactcgcgag
    32641 caacatattc gtcgtgagaa agcgaactcc aacatttgta cttcccaggt actgctggca
    32701 aacatcgcca gcctgtatgc cgtttatcac ggcccggttg gcctgaaacg tatcgctaac
    32761 cgcattcacc gtctgaccga tattctggcg gcgggcctgc aacaaaaagg tctgaaactg
    32821 cgccatgcgc actatttcga caccttgtgt gtggaagtgg ccgacaaagc gggcgtactg
    32881 acgcgtgccg aagcggctga aatcaacctg cgtagcgata tcctgaacgc cgtggggatc
    32941 acccttgatg aaaccaccac gcgtgaaaac gtaatgcagc ttttcagtgt gctgctgggc
    33001 gataaccacg ggctggagat cgacacgctg gacaaagacg tggctcacga cagccgctct
    33061 atccagcctg cgatgctgcg cgacgacgaa atcctcaccc atccggtgtt taatcgctat
    33121 cacagcgaaa ccgaaatgat gcgctatatg cactcgctgg agcgtaaaga tctggcgctg
    33181 aatcaggcga tgatcccgct gggttcctgc accatgaaac tgaacgccgc cgccgagatg
    33241 atcccaatca cttggacgga atttgccgaa ctgcacccgt tttgcccgcc ggaacaggct
    33301 gaaggttatc agcagatgat tgcgcagctg gctgactggc tggtgaaact gaccggttac
    33361 gacgccgttt gtatgcagcc gaactccggc gcacagggcg aatacgcggg cctgctggcg
    33421 attcgtcatt atcatgaaag ccgcaacgaa gggcatcgcg atatctgcct gatcccggct
    33481 tctgcgcacg gaactaaccc cgcttctgca catatggcag gaatgcaggt ggtggttgtg
    33541 gcgtgtgata aaaacggcaa catcgatctg actgatctgc gcgcgaaagc ggaacaggcg
    33601 ggcgataacc tctcctgtat catggtgact tatccttcta cccacggcgt gtatgaagaa
    33661 acgatccgtg aagtgtgtga agtcgtgcat cagttcggcg gtcaggttta ccttgatggc
    33721 gcgaacatga acgcccaggt tggcatcacc tcgccgggct ttattggcgc ggacgtttcg
    33781 cacctcaacc tgcataaaac tttctgcatt ccgcacggcg gtggtggtcc gggtatggga
    33841 ccgatcggtg tgaaagcgca tctggcaccg tttgtaccgg gtcatagcgt ggtgcaaatc
    33901 gaaggcatgt taacccgtca gggcgcagtt tctgcggcac cgttcggtag cgcctctatc
    33961 ctgccaatca gctggatgta catccgcatg atgggcgcag aagggctgaa aaaagcaagc
    34021 caggtggcaa tcctcaacgc caactatatt gccagccgcc tgcaggatgc cttcccggtg
    34081 ctgtataccg gtcgcgacgg tcgcgtggcg cacgaatgta ttctcgatat tcgcccgctg
    34141 aaagaagaaa ccggcatcag cgagctggat attgccaagc gcctgatcga ctacggtttc
    34201 cacgcgccga cgatgtcgtt cccggtggcg ggtacgctga tggttgaacc gactgaatct
    34261 gaaagcaaag tggaactgga tcgctttatc gacgcgatgc tggctatccg cgcagaaatt
    34321 gaccaggtga aagccggtgt ctggccgctg gaagataacc cgctggtgaa cgcgccgcac
    34381 attcagagcg aactggtcgc cgagtgggcg catccgtaca gccgtgaagt tgcggtattc
    34441 ccggcaggcg tggcagacaa atactggccg acggtgaaac gtctggatga tgtttacggc
    34501 gaccgtaacc tgttctgctc ctgcgtaccg attagcgaat accagtaatt cactgattcg
    34561 agtattttct aaaggcgctt cggcgccttt ttagtcagat gacaaagtac aaaagtgctc
    34621 agacagtccc ctcgcccctt tggggagagg gttagggtga ggggaacagg ccggcactgg
    34681 cgcgaatatt taccctcacc ccgaccctct ccctgaaagg gcgaggggga aaagcgtgcc
    34741 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    34801 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    34861 caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc
    34921 tccacgcggc gcaggaagtg atgaacttaa taatgcaagc cggtggcaaa gcattcgtgc
    34981 tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca atcgatcagc
    35041 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    35101 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    35161 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    35221 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa tatgttgatt
    35281 acgcggcatc gaaaggggcg attgattcgt taaccaccgg actatcgctg gaagtcgccg
    35341 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    35401 gcgcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccacc ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctgaca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtctatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 cacat
//
LOCUS       DADLCQ010000013        47696 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000013 DADLCQ010000000
VERSION     DADLCQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.013"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(134..209)
                     /locus_tag="JQG95_000688"
     tRNA            complement(134..209)
                     /locus_tag="JQG95_000688"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Lys,seq:ttt)
     gene            complement(374..1165)
                     /gene="cpoB"
                     /locus_tag="JQG95_000689"
     CDS             complement(374..1165)
                     /gene="cpoB"
                     /locus_tag="JQG95_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY5887351.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1175..1696)
                     /gene="pal"
                     /locus_tag="JQG95_000690"
     CDS             complement(1175..1696)
                     /gene="pal"
                     /locus_tag="JQG95_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY5887352.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1731..3023)
                     /gene="tolB"
                     /locus_tag="JQG95_000691"
     CDS             complement(1731..3023)
                     /gene="tolB"
                     /locus_tag="JQG95_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY5887353.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3156..4397)
                     /gene="tolA"
                     /locus_tag="JQG95_000692"
     CDS             complement(3156..4397)
                     /gene="tolA"
                     /locus_tag="JQG95_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY5887354.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4462..4890)
                     /gene="tolR"
                     /locus_tag="JQG95_000693"
     CDS             complement(4462..4890)
                     /gene="tolR"
                     /locus_tag="JQG95_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY5887355.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4894..5586)
                     /gene="tolQ"
                     /locus_tag="JQG95_000694"
     CDS             complement(4894..5586)
                     /gene="tolQ"
                     /locus_tag="JQG95_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY5887356.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5583..5987)
                     /gene="ybgC"
                     /locus_tag="JQG95_000695"
     CDS             complement(5583..5987)
                     /gene="ybgC"
                     /locus_tag="JQG95_000695"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY5887357.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6137..6430)
                     /gene="ybgE"
                     /locus_tag="JQG95_000696"
     CDS             complement(6137..6430)
                     /gene="ybgE"
                     /locus_tag="JQG95_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY5887358.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6430..6543)
                     /gene="cydX"
                     /locus_tag="JQG95_000697"
     CDS             complement(6430..6543)
                     /gene="cydX"
                     /locus_tag="JQG95_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY5887359.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6558..7697)
                     /gene="cydB"
                     /locus_tag="JQG95_000698"
     CDS             complement(6558..7697)
                     /gene="cydB"
                     /locus_tag="JQG95_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY5887360.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7713..9281)
                     /gene="cydA"
                     /locus_tag="JQG95_000699"
     CDS             complement(7713..9281)
                     /gene="cydA"
                     /locus_tag="JQG95_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY5887361.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10130..12763)
                     /gene="mngB"
                     /locus_tag="JQG95_000700"
                     /pseudo
     CDS             complement(10130..12763)
                     /gene="mngB"
                     /locus_tag="JQG95_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(12781..14757)
                     /gene="mngA"
                     /locus_tag="JQG95_000701"
     CDS             complement(12781..14757)
                     /gene="mngA"
                     /locus_tag="JQG95_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY5887362.1"
                     /translation="MVLFCRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTLQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14867..15589
                     /gene="mngR"
                     /locus_tag="JQG95_000702"
     CDS             14867..15589
                     /gene="mngR"
                     /locus_tag="JQG95_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY5887363.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15692..16561)
                     /gene="sucD"
                     /locus_tag="JQG95_000703"
     CDS             complement(15692..16561)
                     /gene="sucD"
                     /locus_tag="JQG95_000703"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY5887364.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16561..17727)
                     /gene="sucC"
                     /locus_tag="JQG95_000704"
     CDS             complement(16561..17727)
                     /gene="sucC"
                     /locus_tag="JQG95_000704"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY5887365.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17821..19038)
                     /gene="odhB"
                     /locus_tag="JQG95_000705"
     CDS             complement(17821..19038)
                     /gene="odhB"
                     /locus_tag="JQG95_000705"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY5887366.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19053..21854)
                     /gene="sucA"
                     /locus_tag="JQG95_000706"
     CDS             complement(19053..21854)
                     /gene="sucA"
                     /locus_tag="JQG95_000706"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY5887367.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22155..22871)
                     /locus_tag="JQG95_000707"
     CDS             complement(22155..22871)
                     /locus_tag="JQG95_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY5887368.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22887..24653)
                     /gene="sdhA"
                     /locus_tag="JQG95_000708"
     CDS             complement(22887..24653)
                     /gene="sdhA"
                     /locus_tag="JQG95_000708"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY5887369.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24653..25000)
                     /gene="sdhD"
                     /locus_tag="JQG95_000709"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24653..25000)
                     /gene="sdhD"
                     /locus_tag="JQG95_000709"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY5887370.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24994..25398)
                     /gene="sdhC"
                     /locus_tag="JQG95_000710"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24994..25398)
                     /gene="sdhC"
                     /locus_tag="JQG95_000710"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY5887371.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25779..25886
                     /locus_tag="JQG95_000711"
     CDS             25779..25886
                     /locus_tag="JQG95_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887372.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26092..27375
                     /gene="gltA"
                     /locus_tag="JQG95_000712"
     CDS             26092..27375
                     /gene="gltA"
                     /locus_tag="JQG95_000712"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY5887373.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27766..28332
                     /locus_tag="JQG95_000713"
     CDS             27766..28332
                     /locus_tag="JQG95_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5887374.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28383..30842
                     /locus_tag="JQG95_000714"
     CDS             28383..30842
                     /locus_tag="JQG95_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5887375.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30857..31588
                     /locus_tag="JQG95_000715"
     CDS             30857..31588
                     /locus_tag="JQG95_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5887376.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31585..32661
                     /locus_tag="JQG95_000716"
                     /pseudo
     CDS             31585..32661
                     /locus_tag="JQG95_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32768..33859
                     /locus_tag="JQG95_000717"
     CDS             32768..33859
                     /locus_tag="JQG95_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY5887377.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33856..34647)
                     /gene="nei"
                     /locus_tag="JQG95_000718"
     CDS             complement(33856..34647)
                     /gene="nei"
                     /locus_tag="JQG95_000718"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY5887378.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34683..35417)
                     /gene="pxpA"
                     /locus_tag="JQG95_000719"
     CDS             complement(34683..35417)
                     /gene="pxpA"
                     /locus_tag="JQG95_000719"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY5887379.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35407..36339)
                     /gene="pxpC"
                     /locus_tag="JQG95_000720"
                     /pseudo
     CDS             complement(35407..36339)
                     /gene="pxpC"
                     /locus_tag="JQG95_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36333..36989)
                     /gene="pxpB"
                     /locus_tag="JQG95_000721"
     CDS             complement(36333..36989)
                     /gene="pxpB"
                     /locus_tag="JQG95_000721"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY5887380.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37012..37755)
                     /gene="ybgI"
                     /locus_tag="JQG95_000722"
     CDS             complement(37012..37755)
                     /gene="ybgI"
                     /locus_tag="JQG95_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY5887381.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38026..39507
                     /gene="dtpD"
                     /locus_tag="JQG95_000723"
     CDS             38026..39507
                     /gene="dtpD"
                     /locus_tag="JQG95_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY5887382.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39657..41075)
                     /gene="phrB"
                     /locus_tag="JQG95_000724"
     CDS             complement(39657..41075)
                     /gene="phrB"
                     /locus_tag="JQG95_000724"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY5887383.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41072..41581)
                     /locus_tag="JQG95_000725"
     CDS             complement(41072..41581)
                     /locus_tag="JQG95_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY5887384.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41710..41997
                     /locus_tag="JQG95_000726"
     CDS             41710..41997
                     /locus_tag="JQG95_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY5887385.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42052..42432)
                     /locus_tag="JQG95_000727"
     CDS             complement(42052..42432)
                     /locus_tag="JQG95_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887386.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42435..47099)
                     /locus_tag="JQG95_000728"
                     /pseudo
     CDS             complement(42435..47099)
                     /locus_tag="JQG95_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(47118..47339)
                     /locus_tag="JQG95_000729"
     CDS             complement(47118..47339)
                     /locus_tag="JQG95_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY5887387.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
BASE COUNT        11339 a        13083 c        11566 g        11708 t
ORIGIN      
        1 actacgctgg atactacaac taactatgtt agtggtgggt cgtgcaggat gactcgactt
       61 cgtctcgccc ttcggcgccg ttgctgatgc aacgttatcc ttcacgtttt actaccgctt
      121 ccaccttagc gattggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc
      181 aactgctcta ccaactgagc taacgaccca ctttttcgtt gctttcggtt tgtttgatat
      241 cccgtggcaa cggcggcata tattactgat ttcagacttg agcgcaacaa aaatttcgat
      301 gcagatcact caactgctta tgattcgcac gacacgacca gaaataatgc gacttctggt
      361 cgtgtgttat gcattacatc gcgttcagac gtttttgtgc ctgtttagcg ccatcagtac
      421 cagggtattt actgataacc tgctggtata cggctttcgc ttttgcggtg tcacctttgt
      481 cctgcatgat gacgccgact ttaaacatcg catcagcagc ctttggtgac ttcggatagt
      541 ttttcactac cgaagcaaaa tagtacgccg catcatcttt tttacccttg ttgtagttta
      601 actgacccag ccaataattg gcgtttggca ggtaagttga atcagggtaa tttttgatga
      661 aattctgaaa tgccaccatt gcgtcatcct ggcgggattt atcctgcacc agcgcaatag
      721 ctgcattgta atccgtgttt gcatcgccgc ttttcaccgg cgcgccagca ttcgcagtac
      781 cagcatcagc tgtcggcgtc gttgatgccg tcgcaccgct ttgatcgctg ctggttgatt
      841 gcgccgctgc accaccgctg ctgaggctgt cgatctgcaa caggatctgc ttttgccgct
      901 ccacgacctg attcagttga tactgatttt cctgaatctg accacgcagg gaatcaatat
      961 cggattgatt atcagaaagt tgttgctgga gttgggttaa aagctggctg tgagcattag
     1021 aaatacgctc aagttgagtg acgcggtctt cgaccgagcc tgagccgaca ctactgattg
     1081 gtgcctgagc aaaagcggcc cagggggccg ctataccaac cagtaacgac agactcaata
     1141 gttgatgtct gaagttactg ctcatgcaat tctcttagta aaccagtacc gcacgacggt
     1201 ttttggagta tgccgcttcg tcatgaccca gtactgcagg tttttcttta ccgtaagaaa
     1261 cgatggagat ctggtctgca gaaacgcctt taccctgcag gtacatctta acggcgttcg
     1321 cacgacgttc acccagggag atgttgtatt ccggagtacc acgttcgtcc gcgtgacctt
     1381 ctacggtgac tttgtaagac gggttgctac gcaggaagtt tgcatgtgca tccagcattt
     1441 gagcgaagtc agaacggata tcgtacttgt ccagatcgaa gtaaacgatg ttgttctgct
     1501 gcagctgttg catttgcaga cgagcctgct cttcggaaga catgttgccg ttgccgccgt
     1561 tcgcatccat accagtgccg gcacccagca tgccttcgct gccgtcattg ctggcgttct
     1621 tgttggaaga acatgccgca attgccataa caggcagagc aatcatcagc cctttcagca
     1681 ctttgttcag ttgcatttca atgattcctt tactattcaa ttaattatta tcacagatac
     1741 ggcgaccagg cagggaattt gacctgtcca tcagttgccg gaagacgcgc tttgaaacgc
     1801 ccatctgtag aaaccaaatt cagcacggat cccatcccct gagaagagct gtagattacc
     1861 atagtgccgt taggtgccag acttggcgtt tcatccagga acgtggacga cagaacttgt
     1921 acgcctcccg ttgccagatc ttgtttggca atgtgctgct gcccaccatt ggagctgacc
     1981 attaccataa atttaccgtc gctgctgaca tccgcatcct ggttctgcga accttcccag
     2041 gtaatacgtt gtggcgcacc gccgttgata ttcactttat aaacctgcgg acgaccggcc
     2101 tggtcagaag taaatgccag gttctggcta tccgggaacc aggtcggttc ggtattgtta
     2161 ctgcgaccat cagtcacctg gcggatctga ccagaagcca aatccattac gtacaggttc
     2221 agactaccgg ttttcgacaa ggcgaatgcc agtttgctgc cgtctggcga gaatgcaggt
     2281 gcaccgttgt gacgcgggaa tgaagccacc tgacgtacag cgccatttgc cagcgtctga
     2341 ataaccagcg cggaacgacc gctttcgaag gtcacataag ccagtttaga accgtctggt
     2401 gaccacgccg gagacatcag cggctgcggt gaacggtgaa cgacaaactg gttgtaaccg
     2461 tcatagtcag atacgcgcag ttcatacggg aactgaccgc cgttggtctg aacaacgtag
     2521 gcaatacggg tacggaacgc acctttaatg ccggtcagtt tttcaaacac ttcatcactg
     2581 gcggtatgac cagcataacg cagccactgc ttgttcactt tgtacgagtt ctgagcaagt
     2641 acagtacccg gtgcgccgcc agtgtcaaca agttgataag caacattgta agaaccatcc
     2701 ggattcggag tgacctgacc gacaactaca gcgtcaatgc ccagtgcgga ccatgcagct
     2761 ggttgtactt cctgcgcact acccggctgc tgtggcagac gagcgcgatc taacggatta
     2821 aatttaccgc tgttacgcaa gtcagcagca acgatgccgc caatatcttc aggtgccgca
     2881 ccaggccccg cccactggaa aggaacaaca ccaataggac gaccggaatc tacaccgctg
     2941 tcgatcacaa tgcggacttc agcatgcaga actgatgccc acagtatgag aaaaccaaat
     3001 gctactcgta atgcctgctt catcatatct cccttatctg gaccgatggc ccacgataat
     3061 ttagcagaat gttaacaaac taaaatacac aaaactacca gaaccccgtg gcaaccggtg
     3121 cctgatgttg accgtccgaa cagtcaacat cgcgattacg gtttgaagtc caatggcgcg
     3181 tttttgaaca cttcatatac tgcctggctt ggtggtttcg ggatcttcgc aagtttagct
     3241 gctgccaacg cagcctgaca aagtgcggga tcgccacctt caggtttgat atccagtaac
     3301 ataccatcgg gtgccagttt tatgcgcagc gtacaggttt tgcctgcata ggacgatgcg
     3361 tcatagaact tactttcgat agcagattta atctgcccgg catagttatt gatatcggcc
     3421 cctgatgcgc cattgttttt agtattacca ctcccggcag gcgaagcatt gttccctttc
     3481 gcccctcccc ccgttttcgg tgcattctta ccagagctta gctcaccgaa aatatcatct
     3541 gcctctgcgg cagcttttgc tgcagcggct ttttcagctg ctgcttttgc cgctgccgct
     3601 tttttgtctg cggcggcttt ttctgccgct gctttcttat cagctgctgc cttatcagca
     3661 gccgcttttt ctgctgcttt tttgtcggct gcagctttct ctgctgccgc tttcttatca
     3721 gctgcagcct tttcagcagc cgctttcttc tcagcttctg ccttggcttt ttcagcagct
     3781 tcagttgctg ctttctttct tgcttcagct gcagctgctt ctgccgcttc cgctttcttc
     3841 ttcagtgccg cagcggctac ctcggctttt ttctgcgctt cggctgcggc tttggcggct
     3901 tctgcttctg cttttttctt cgcgtctgca gccgctttct tcgctgcttc ttccgcagct
     3961 ttagcatctg ccgccgcttt cgctgccgcc acttcagctt gcttctgctt taactcggcc
     4021 tgttttgcgg cttcttcagc ctgctttttc tgctcctgag ccgctaaccg ctctttctca
     4081 agttgcttca ggcgttcctg ttcagccgct tgtttctcac gcagttcttc agcagcctgc
     4141 tgttccttca tcttgcgctg ttcatcagaa cgcttcgcgc ttgattcctg gctttgcatg
     4201 cgtttgtact gctcaactac cgcacctgaa tcaaccatga cagcgtcgat ggacgaacca
     4261 ccgccgcctc cggctgaagc ttctatattc tcatcgaacg aactccagat cagcgccgca
     4321 aataagatga catgcagcac tgctgaaata attatcgccc gcttgagctt gtcgttttgt
     4381 tcggttgcct ttgacactct cggtttccaa aaactgttcg cctgttaccc gctctctttc
     4441 aagcaaggga aacgcagatg tttagatagg ctgcgtcatt aaaccaaccg atttcacacc
     4501 cgcactatgt aacaagttca gtgctttaat tatttcatcg taaggcacat cttttgcgcc
     4561 accgatcaga aagaccgttt tcgggttggc cttgaaacgg ctggacactt ccgccaccac
     4621 ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc accacggtgt actgaccaat
     4681 accagacact tcaacaatca ctggcggatt atcgttactg ctcaccgcct gtgattcagt
     4741 agcgtctggc agatcgacct ccacgctctg ggtgatgatg ggcgctgtcg ccataaagat
     4801 cagcaacagc accagcagta cgtccagcaa cggaacaatg ttgatttcgg acttgagatc
     4861 gcgacgacct cgtccacgcg ctctggccat ggcttacccc ttgttgctct cgctaacggt
     4921 aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata aagttgtcgt aattcagttc
     4981 cagtttgttt acgcgctggt tgagacggtt ataagccatt accgccggaa ttgcggcgaa
     5041 cagaccaatt gccgtcgcaa tcaacgcttc tgcgataccg ggcgcaacca tttgcagtgt
     5101 tgcttgtttt accgccccga gggcgataaa ggcgtgcatg atcccccaga ccgtaccaaa
     5161 cagaccaata tacgggctga tggagccaac cgtaccgagg aacggaatgt gcgtttccag
     5221 attttcaagt tcacggttca tggagatacg catagcacgc gacgcccctt ccactacggc
     5281 ttccggcgca tggctattgg cacgatgcag gcgcacaaac tctttgaacc cgctgtaaaa
     5341 gatttgttcc gaaccagtca gattatcccg tttcccctga ctctcttgat agaggcgaga
     5401 gagttcgatt ccagaccaga atttatcttc aaacgcttcg gcttcacgcg ccgctgcgtt
     5461 aagaatacgg gttcgctgga taataatggc ccaggatgcg attgaaaaac caatcaaaat
     5521 caacatgata agtttaacca gaaggctagc cttcaggaac aaatcaagga tattcatgtc
     5581 agtcactgct taaactccgc gacaatagac ttgggaagcg cacgaggctt cattttgagt
     5641 gggtcaacgc aaacaaccag aacctctgct tcattcagca aagtattctc ggcgttgaca
     5701 atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg ttatttcagt ctgtatttcg
     5761 agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg tcattttacg taccacaaag
     5821 gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat gatgacgcag catctctgtg
     5881 cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca ccacaccacc ggcatcggta
     5941 tcttcatagt agacgcgaac cggccatcga aacagcgttg tattcacttt acatcccggt
     6001 aatgcaacaa aagttagagc ttttaaacgc tgctactata cgcgcgcgat tgaggtttgg
     6061 gaatgagggc aagttaaggg cgcgtaaaaa tttatgggcc tggagcggcc cataattttt
     6121 taaagagaat tctgatttaa aagaagaaga aaatcagccc gacaatgaga acaatatcgg
     6181 caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg cggacgaaag cccacgccgt
     6241 gaatcacacc agcacagacg gcccacatca gcaataaacc atgccagatt tccagttcac
     6301 tggtcttcgc ggcaaaacga gatgggtccc aaaacataca tcctgctaac agaagcgcca
     6361 tcacgaagga aagcgcccgc agggggcgct tgtccattac cgcatacaag gtcgcgataa
     6421 tcttactcat cagatgtctt cttgaccggc tttgcctgat tcgacgtgtt caagcgccag
     6481 cgcggtgatt accccaaacg aacaggcaag aagcgttccc agaatccatg cgaaatacca
     6541 catttttagc tccttactta gtacagagag tgggtgttac gttcaatatc ttctttggtg
     6601 atacgaccga acattttcca gtaacaccag gcggtgtaga gcagaatgat cggtaccaga
     6661 accaccgcaa cccaggtcat gacgttaagc gtcagctggc tggaagttgc atcccacatt
     6721 gtcagacttg cgttcatcat ggtgctggac ggcatcacaa acgggaacat tgcgataccg
     6781 gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca caaacgccca cgcggcttta
     6841 tccatacgtg cagtcaggat ggtcagcagc agcagaacca cacccagtgc cggaatagcc
     6901 cacagaattg gcgtgttgtt gaagttaacc agccatgcgc cagcttcacg aaccacttct
     6961 ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg atttcacgac ataaccatcg
     7021 ataccgtaca tcacccatac gccagccagt gcgaaacaga ccagtgtcac cagcgcagcc
     7081 acctgagccg ttgcacgggt acgcaggtgc agttcgccca cggtacgcat ttgcagatag
     7141 gttgcgccct gagtaatgat catccctacg ctcaccacgc ctgccagcag gccgaacggg
     7201 ttaagcaact ggaagaagtt accggtgtag tacagacgca gatattcatc aacgttgaac
     7261 ggtacgccct gcaacaggtt accgaacgct acaccaatta ccagcggcgg aacgaagcta
     7321 ccaatgaaga tgccccagtc ccacatgtta cgccagcggg tttcttcaat cttggagcgg
     7381 tagtcaaaac cgaccggacg gaagaacaaa gacgccagca cgaggatcat cgccacatag
     7441 aagccggaga acgcagcggc atagaccatc ggccaggcag caaagagtgc gccgcccgcg
     7501 gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa tggagttaat cataattcga
     7561 cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc ccacccccat gtcgaaaccg
     7621 tcagtgactg caaaaccaat cagcagaacg ccaaccagca gccaccagat aaaacgcaat
     7681 acttcataat cgatcatttg acgactcctg tcttagcgtg ccggctgagt agtcgtggaa
     7741 gactgctcaa agtgatagcg accggttttc aggctgcttg ggccgaggcg tgcaaacttg
     7801 aacattaaga acaattctgc caccaggaac agggtataca ggccgcaaat cagcaccatt
     7861 gagaagatga gatcgcctgc ggtcagtgac gaattcgcca cagctgtcgg cagcacttca
     7921 ccgatagccc acggttggcg gccatattca gccacgaacc agcccgcttc tacagcaatc
     7981 cacggcagcg gaataccgta cagcgcggcg cgcagaagcc attttttctc gccaatgcgg
     8041 ttgcggatga cactccagaa agagagcgcg atgattgcca gcagcaggaa gccacacgcc
     8101 accatgatac ggaacgcaaa gtacagcggc gctacacgcg ggatggagtc tttggttgcc
     8161 tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg tatagcgttt cagcagcaga
     8221 ccgtaaccga ggtctttctt catgctattg aactggtcac gaaccgcctg gtcggtagaa
     8281 ccagaacgca gttgttcgag cagagagtac gccttcatcc cgttacgaat gcgttcttca
     8341 tgctgcacca tcagctcttt caggccgata accggggtat ccacggaacg cgttgcaatg
     8401 atgcccagtg cgtaagggat ctgaatcgca aatttgttcg tctcctcttc ctgatcagga
     8461 atgccgaaca aagtaaaggc agcaggcgca ggttgcgttt cccactcggc ttcaatagca
     8521 gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg attcatcacc cagaacaata
     8581 acagacagaa cagcagccat accgaagctg gcagcgatag caaaggagcg tttagcgaag
     8641 gcgaagtcac gacctttcag catataccat gcgctgatac cgaggatgaa catcgcgcca
     8701 gtcacataac cagacgctac agtgtgaacg aatttcacct gagcaaccgg gttaagcacc
     8761 agctcggaga agctcaccat ctccatacgc atagtttcaa agttgaaatc ggacgcgatt
     8821 gggttttgca tccagccgtt cgcaaccaga atccacagtg cggacaggtt tgaaccgagc
     8881 gccaccagcc aggtgacaca catatgctga actttaccca gacgatccca accgaagaag
     8941 aacagaccta caaaggtgga ttcgaggaag aaggccatca gaccttcgat tgccagcggc
     9001 gcaccgaaga tatcccctac atagtgggaa tagtaagacc agttagtccc gaactggaac
     9061 tccatggtca gaccggtagc cacacccaga gcgaagttga taccaaacaa cttgccccag
     9121 aacttggtca tatctttata aatctgtttg ccggagagga cgtagaccgt ttccataatg
     9181 gccaggagga acgccatacc gagcgtcagt ggcacaaaaa ggaagtggta catcgcggtc
     9241 aaggcaaact gtaagcgcga cagttcgact atatctaaca tcatgactcc ttgctcatcg
     9301 catgaagact ccgagagtga accccgttag aaagggtcac acgcatgccc caatagaaat
     9361 ttgtttgaac actttcatca taccatgtga atgcacacaa aaacggcgta aagaaaggtt
     9421 gcaaacatgt taataaaaac tcaaattgat cccacgtata tattacgccg caaaatcctt
     9481 acaataaaca ggtttttgtt gaagcaaatt tgcatttttc gacggtgatc aatttatagc
     9541 taaattgccg cctttcagcc aatttgatcg agaacaattt atctcttttt gatgcccatt
     9601 tccaagactt atacattgat aaatatcaat ttctacaaat tatgttaatc atgtttcaac
     9661 aaggtaactt tagtgatatt tatgtttaaa atgtgtgaat tataagactg aaattagttt
     9721 cggttggcaa ttaaagataa aaagagacga agcaaaaaca gcacggttga ccttaattct
     9781 ttttttggtc actggctaat acgacgcaga gtttcaggat tattaataaa ggcataaatc
     9841 tcccccccgc cgatattaac atttaattaa cttataatta tattataatt aactcacccg
     9901 tggttattat tttgttaaaa ataaagccac atatacgtag cttgagagtg aagataaaca
     9961 caaagttacc tgatgcgcta cgcttgccag tgtctacatc atctctgcaa tatattgaat
    10021 ttgcctgctt ttgtagaccg aataaagcgt tcatgccgta tccggcatga acaaagcgcc
    10081 ctttgtcaac aatctggccg cgcatatgtg gcttcggttt gattgctatt caggcaagcc
    10141 ggtaactgaa cgtccgtgac tggccgggta aaaaaggccc cgatagattt gaaccttgat
    10201 tttcctcggt ggtaatgtgt tcatccatca tcgtttctga gcaagaaatc acctcgcgac
    10261 tgaaagcaac agtcgcatca caggttgctg attcagccgg attaaacagc cgtaaaatca
    10321 cttcttgtcg gtcttcagct ttcttaagtg cgcttatcag gcatcccact gggggcattt
    10381 tcaacaaact ataactttcc ggcacgttga atccggcttt gttgagcttc atcgcatccc
    10441 atgggatttt gttgtaacac tgtactggag tcagccatgc tcgcgcctgc tgagctacac
    10501 cagcggcggt tggcgtaccg gtataactca ataaacttag gcgacaagaa agcagaccac
    10561 gtagttgtga gtccgggact ggcattttaa ttcccgaagg ccgcctaggc cttaaaagca
    10621 gatcttcttt ccccagtaag cccacgccgc gcagcaacgt aatggcaaag gttttcttct
    10681 cttcaccgat gacttcaaat tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc
    10741 cttcctgtaa ggctgcatag ttgagcatat tccataccgg aaccggcgct tctttccagc
    10801 cttcttgctg ccagttgttc attgcactgt cgttcacggg gcgcgttagc gaaccaaact
    10861 gggtatctgc cagaacacta tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat
    10921 gatcgtcagc ctggttatca aggttgatat ccacatcaat gcgcctgttg ttatgactaa
    10981 gagtgacaac caactcgaca tctactctgc cagtggattg tcttgcgctg cgttgttgca
    11041 gattacgcgg caccgccatt tcatagcgga taacagccct gctctgccag gcctcatgaa
    11101 taatctcgca ttgcggtttc gcattcgccg aagtaattac ccactcttct tttgcgggtg
    11161 aatagtcata ttcatcacca tcatcagagc tttcttcaat ttgcaacacc cggtcatagc
    11221 gcacaccgct atctttatct accagttgta gcgttccatc ctcattgagc gcaatttgcc
    11281 agaaagcatt ttccagtatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct
    11341 gattcgcttc gatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgta
    11401 tatcaaactc cataaaggga tcgtaattac cgtaatgaac tatttgccga tcgattaggc
    11461 ctggatcgat ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctc
    11521 gtagattaaa ctggctggcg cgcagccgca cagtggtgtt gatcacttct tcccgcggcc
    11581 agggcatcag gttaaacagg acgagtttgt cggcgtcgct ctgcggcatg ttgtcggcga
    11641 ttttacgcat atagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg
    11701 cgacgatttc gcgatgaact ttgtcactac agcagcagcc gatgctgtcg tgggcatgat
    11761 tttttaagat ctctttccac attttctcca gcaagccgtg gtggtatcca aaacccaacg
    11821 tcctggccag cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt
    11881 gggcaacttt gatatccatg cgcgtggaac cgatggtgcg atgcacgcgc atatatttgc
    11941 catcaataaa ttcccctttc agggttgcca gattatctcg ctgcgcttcg atcttctcaa
    12001 atacctcttc aaagcggctc atcacaaact ttcgttgagg ataaatttcg cgcagtttct
    12061 ccatcacttc gaagatattt tgctgcaatg gcatctgatc atgcccattc ggcagcaaaa
    12121 tctctttggt tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttgcgta
    12181 atccgttttc gtccgcaggt aagtacttac cgatggcata acccaacggc atcacctgcg
    12241 ccgtcacttc gctaccgtca ctgctttgcc acaaaaactc ggttttatcc gtaccgtggc
    12301 gctcagaaca tccacgccag aacatggtgc aggtaatgcc aaatccattg tagatatgcg
    12361 gcagttgccc ggacatgcca aaggaatcgg gtaaataacc tattttcatc ggctcgccaa
    12421 acgcgaggca gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa
    12481 tcgtggtatc ggtctgggta tactaggggc cgataatcaa cttgccggct tctaccagtt
    12541 tcttcacgcg gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt
    12601 gcccgtcgag tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt
    12661 ccatattatt gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc
    12721 agtgcatatg cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt
    12781 ttatggcatt acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat
    12841 caggagaatt gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc
    12901 cgccataaca ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccagcacc
    12961 gggtgtcgaa aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag
    13021 cacaaacgaa ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt
    13081 aatacctgcc agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa
    13141 caggcgcggt gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga
    13201 ggcgaaaatg gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata
    13261 agcggcttta ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc
    13321 cagcaacagc gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat
    13381 ccaggcgaca ggttccccca ccacaaacag catcagactg cccgctccca atgtaccgag
    13441 caccgggtag agataaaaag tcaggaagcc attgaattta ctgctaagac gcaagtgatt
    13501 tttcatccag cgtatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc
    13561 ggagccgatc atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc
    13621 tgccagcgaa taggcggtat aggctgccag caccggtacc atcaaaattc cgagcagccc
    13681 gccgcccagt ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag
    13741 cccgaagatt tgcgacagta ataccgcgac cgccagcacc gtcccccccg cgacaatcaa
    13801 cggtacggca aaagagatcc cgctcaacag tgcctgtttc agctccgttt tgacgctttt
    13861 caccggttgc gtatcttgtt gtaaggtacg cgtctcatcg ccacgctcga gggtaagcga
    13921 ttgttggatc aacgcttctg catggcgaat cggctcggca acaggcacag aaagcgcagg
    13981 aatgccgtta aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcagt
    14041 tgcactattg agttgatccg ccgttaaacg cccttcaata ccgttagcac cttgcttttc
    14101 aacgtagaca ttcacgccga gtttgcgtcc ggctttttcc agatactccg cagccatgta
    14161 ggtgtgagca atacccgccg ggcaggccgt tacgcaaacg atagttggcg cgttggaaaa
    14221 agaggcagaa ggttgcgtgc ctcccttgtc atccagcgcc gagagcaatt catcaggcgt
    14281 cgtcgccgac tgtatacgcg cccgaatctc atcatccgcc aggcgcgtgg tcagcgctgt
    14341 cagcagttgc atgtgcgtag tacccgcttc attgagggga atcgccagca acaccactaa
    14401 atcaaccgct tccgggccat ccacgccttc ccactgaagc ggctcgctga gtgtggcgac
    14461 cgcaaacgcc gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcact
    14521 taaggcggtc gggccaaggc tttcacggcg atacacttct ttcagaaatt gctcagtact
    14581 ggaaattttc cccagagcag caagacgttg agtcaacgcg tgaatggcct cttcacggct
    14641 ggtaaagcgc gcattcagac acaacgcatc gcggtgggtc agagtcgtca ggttcatcat
    14701 gatcctcact tgatcgtttt tatagtcgcg ccagtgtgcc cgacaaaaca ataccattgc
    14761 gtaatcaatt tcacttgatt gaaattattt gcattttatt tgtattgttt acaggatgaa
    14821 tttatcgggc ataatggcta aattcgtatt aatgagatgc taaataatgg gacacaaacc
    14881 cttataccga cagattgctg atcgcattcg tgagcaaatt gcgcgtggcg agcttaaacc
    14941 tggcgatgcg ttacctaccg aatcggcatt acaaacagag tttggcgtca gccgggttac
    15001 ggtgcgtcag gctttgcgcc agttagtcga gcaacagatc ctcgaaagca ttcagggcag
    15061 cgggacttac gtcaaagaag agcgggtcaa ttacgatatt tttcagttaa ccagttttga
    15121 tgaaaaactg tcggaccgtc acgtcgatac acacagtgaa gttctgatat ttgaagtgat
    15181 tccggctgac gattttcttc agcaacagct acaaatcacc gtgcaggatc gtgtgtggca
    15241 tgtgaagcgc gtgcgctatc gcaagcaaaa gccaatggcg ctggaagaaa cctggatgcc
    15301 gcttgcgttg ttcccggatc tcacctggca ggtcatggaa aattcgaaat atcactttat
    15361 tgaagaagtg aagaagatgg ttatcgatcg tagcgaacag gaaatcattc ctctgatgcc
    15421 aaccgaagag atgagccgtc tgcttaatat cagccagaca aagccgattc ttgaaaaagt
    15481 atcgcgtgga tatttagttg atggtcgggt gtttgaatat agccgcaacg cttttaatac
    15541 cgatgactat aaatttaccc tgatagcgca gcgaaaatca tcgcgataag cacaaaaagg
    15601 ccatcatacg atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga
    15661 agcggcgagg gctatttctt attacagata tttatttcag aacagttttc agtgcttcac
    15721 cgatatccgc caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct
    15781 catccgcagt ccctttccca ccggcaatga tcgcacccgc gtggcccata cgtttgcctt
    15841 tcggcgcagt cacaccagcg atgtaaccca caactggctt ggtaacatgc tctttgatgt
    15901 acgctgccgc ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg
    15961 tctgcggatc tttttcgaac atttcgagaa tgtcgataaa gttagagcca gggatcgggt
    16021 caccgccgat accgacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg
    16081 cttcataggt cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt
    16141 gacccggctg gataccaatt ttgcattcac ccggggtgat aacgcctggg cagttcgggc
    16201 cgatcatacg aacgcctgct tcgtccagtt tcactttcac ggtcagcata tccagcgtcg
    16261 ggatgccttc agtgatggtg ataatcagtt tgatgcctgc atcgatagct tccagaatgg
    16321 agtctttgca gaacggtgct ggtacgtaaa taacagacgc ggaagcgcca gtagcagcaa
    16381 cagcttcacg cacggtgttg aacaccggca ggccgaggtg ggtagtgccg cctttacctg
    16441 gagttacgcc accaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc
    16501 cctggctacc ggtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca
    16561 ttatttcccc tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc
    16621 aataatattc aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc
    16681 cagacgtacc acgaccggta cgttaacacc cacttccgct accgcgccga tgataccgtc
    16741 agcgatcagg tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt
    16801 gtcgtcagag aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc
    16861 aacgtccagg aagttagccg gttcgccgcc gtgcagttta actatgtcca tcgtacccat
    16921 cgccagacct gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag
    16981 ttcccactgt gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg
    17041 cagatcaggc tggcggaaca gggcgttgcc atcagcgccc agtttgccgt cgaggcaaat
    17101 cagatcgccc tgtttggtga tgaccagcgg gttgatttcg atcagcgcca ggtcgcgctc
    17161 caggaaaatg gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc
    17221 cagacccagt ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc
    17281 aagcgcaact ttatggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc
    17341 gccttcggtg gaggccataa agaccacgcg acgggaacta cggtctacaa cggcaccgag
    17401 atacagctct ttagcgatat cggtcgccgc ttcaaccaga atctggttaa ccggttggcc
    17461 attggcatct gtttgatacg ttaccagacg cttgcccagc cagttttctg caaaagcacg
    17521 gatgtcttct ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg
    17581 aacctgacat ttcactaccc acggaccggc accgattttt gaagcggctt cttctgcttc
    17641 gcgcggagta gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaacagttg
    17701 ttttgcctga tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt
    17761 ttaaaccgta gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta
    17821 ctacacgtcc agcagcagac gcgtcggctc ttccagtaac tctttgatcg ttaccaggaa
    17881 gcccacggat tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat
    17941 cggcaggatc tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc
    18001 cagaattgcg ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc
    18061 accgttggtg atggtgaagt taccaccggt cagatcttca accgtcagct tgccatcacg
    18121 gcctttgact gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac
    18181 atcacgcaga accggcgtca ccaggccgcg cggcgtagaa accgccatgc tgacgtcgaa
    18241 atagttgtgg taaaccacgt catcgccgtc gatagacgcg ttcacttccg ggtaacgttt
    18301 cagggcttca accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg
    18361 tttttcaaac gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc
    18421 gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg
    18481 cttacgcagg cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc
    18541 cgccggagca gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc
    18601 cacatcttca cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag
    18661 attgtgttca gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc
    18721 cagagacgcc tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt
    18781 ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt
    18841 tgtaccttca tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag
    18901 taccactttg tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg
    18961 ttttttatgc caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag
    19021 aatatctacg ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc
    19081 agatcttgtt gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc
    19141 gggcggcctg cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg
    19201 ctgcagtacc atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg
    19261 tgagcaaact gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg
    19321 atacgcacaa tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa
    19381 taaaccttac cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt
    19441 tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc
    19501 ggatgacgca gcagggattt cggcgacatc acgaccagcg gacgacgcat cccgcgcagc
    19561 gcctgacgac gcagcatgtg gtaaacctgt gccggggtag acggtacgca aacctgcatg
    19621 ttttgctcag cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc
    19681 tgcccttcgt aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgc
    19741 tcgccagagg agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac
    19801 tgcgcttccc agatggtcag ggtgcgtggt tctgcggtgg cataaccata ttcaaacgcc
    19861 agcacggctt cttctgacag tacggagtcc cagacacgga acgcgccctg accgttatgg
    19921 atgtgttgca gcggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg
    19981 tggaagaagg taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcatca
    20041 accagcgtgg cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggct
    20101 gccatcgcct ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttct
    20161 ggtaccgtac tgatacgttt agccagttcc tgcaggcgct tcatctcaac tttgttcggg
    20221 tactcttcgt cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc
    20281 ggacgccact ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc
    20341 atctcggtgg catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt
    20401 ttgcgcggtg tcggatgttt tttgattttc tgatacatca gtggctgggt tgcgctcggc
    20461 tcgtcggctt cgttgtggcc gtgacggcgg tagcatacca ggtcgatgaa gacatcacgt
    20521 ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca
    20581 tccgcgttaa cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt
    20641 gtagaacggg catccagcgg gttagaggtg gtgaaaccaa cctggttgtt gataacgata
    20701 cgtaccgtac cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc
    20761 acgccctgcc cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg
    20821 ttcggctcat caagtctgtc cagacgggca cgaacagagc cgataacgac cgggcttaca
    20881 atctcaaggt gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg
    20941 aagtcagacg agaagcccat gtggtatttc acgtcgcctg tgccgaggtg ttctttatgt
    21001 ttaccggcga actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg
    21061 ttcagacgac cacggtgcgc catcccgaga accacttcgc gggtgccgct gttgccagcg
    21121 tggcggatca tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt
    21181 tttgcgccag ggaattttgc gccgaggtaa cgctcaaggc cttcagcggc ggtcagttcg
    21241 cttaagaagc gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt
    21301 tggatccagc gtttttcttc ggtgctggtg atgtgcatat actcggcacc aatcgggccg
    21361 cagtaggttt gcttgagggc ggaaataagc tcgccgagtt tcatggtttc tttgccgctg
    21421 gcaaatgaac cgacgttgaa ggtttcctgg aagtctgctt cggtcagatc gtggaaagac
    21481 ggatccagat cggccacttt atcttgctgc cacagtccca gcggatctag attcgcatgc
    21541 tggtgaccac ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg
    21601 gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa
    21661 tattcacgcg tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg
    21721 aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc
    21781 tgttctatcc agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa
    21841 gcgctgttct gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac
    21901 taccacgcac agtgatgtgc gtgccggggt tcacttgcga gctcttgtct acgtagtggc
    21961 tcgcgaagga acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc
    22021 atttctttgt tacgcctgat gcgctgcgct tatcaggcct gcgggtaatg acttgtaggc
    22081 cggataaggc gttcacgccg catccggcac tggttgcctg atgcaacgct tgcgcgtctt
    22141 atcaggccta cagtttacgc attacgttgc aacaacatcg acttgatatg gccgatggcg
    22201 cgcgtcgggt tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg
    22261 aatacgctga atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta
    22321 tcgatcagga aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc
    22381 caccagaaag acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg
    22441 tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca
    22501 ttattcaaca ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc
    22561 accaaatcgc ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc
    22621 tggttgagtg ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga
    22681 ccgtcggaac cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc
    22741 tctttcagct ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg
    22801 gtgtaatcct gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac
    22861 tcgagtctca ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg
    22921 cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg attccgactc aggcagatac
    22981 agggagtggc acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg
    23041 ccacggcttt cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg
    23101 ttatccagtt ccaggcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga
    23161 cgggcatttt tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca
    23221 tcaccttcac ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacggatc
    23281 gccaccggat cttcaccgtt acgattattg ttccaccggt tcaggcgatc cagagacgct
    23341 tcaacatcag actcgctggc atcgtgcagt gcgccctgct cggcgataga ctcttgcaga
    23401 tgcagacctg ccgcgcgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg
    23461 ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg caaacagtcc cggaacaacc
    23521 acatcttcgc ctttctcatt cacagtcagc gcctgaccgg taactttggt cggaataccg
    23581 cccatcatgt agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga
    23641 gcgaaggtac gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc
    23701 aggtgatcga gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca
    23761 cggatttcaa tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc
    23821 ggcgcataac gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca
    23881 cggcaacctt cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac
    23941 atttccatat cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg
    24001 ttaatgtggg cgttagtggt ggactgataa atacgccctg ctccgccagt cgccagcacg
    24061 gtagcgcggg ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc
    24121 accaccgcgc catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg
    24181 gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca
    24241 gcagccgccg cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac
    24301 ggacgttgat agatacgacc atcatcgaga cgcgagaacg gcaggcccat gtgttcgagt
    24361 tccagaatcg cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata
    24421 tagtccgacc cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta
    24481 cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact
    24541 ttagagagca gggcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct
    24601 gcgccaccgg caccaatcac aactgcatca aattctctga ctggcaattt catcacacac
    24661 cccacaccac aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt
    24721 gcagcatcag gcgcaaggcc agcggtttaa cgtagtcggt caacacctgc cacatgccga
    24781 tccaggcatg aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag
    24841 aggcgaagaa accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga
    24901 ccatataaat gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat
    24961 gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag
    25021 tgaaagcacg acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt
    25081 ttcttccaga tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc
    25141 cagagcggta aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga
    25201 agcttgctcg aaaccttcag gggaagagag gctggtaccc agaagccact gcaggatgcc
    25261 cactgcaaca aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat
    25321 ggggaaccgg atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat
    25381 catgaataac gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta
    25441 actttctgtt acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca
    25501 gcgtcctttc cataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg
    25561 ttacaacgct gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc
    25621 tgttcacaaa atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt
    25681 gatccaggtc acgataacaa catttattta atttttaatc atctaatttg acaatcattc
    25741 aacaaagttg ttacaaacat taccaggaaa agcatataat gcgtaaaagt tatgaagtcg
    25801 gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga
    25861 cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt
    25921 attcgtcatc aatggattct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc
    25981 ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg
    26041 caaatttaag ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat
    26101 acaaaagcaa aactcaacct caacggggac acagctgttg aactggatgt gctgaaaggc
    26161 acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt
    26221 gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat
    26281 gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac
    26341 ctagaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa
    26401 tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg tctgttccac
    26461 gctttccgtc gcgactcaca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg
    26521 gcgttctatc acgactcgct ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc
    26581 cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag
    26641 ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc
    26701 tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt
    26761 ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc
    26821 tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct
    26881 gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa
    26941 cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc
    27001 ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc
    27061 catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg
    27121 gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat
    27181 ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt
    27241 ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagttacggt
    27301 atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc
    27361 gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg
    27421 gcgggttaat ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc cactatcgga
    27481 cattcatata ttctaaaaaa aaatacatcg gtataaatta taaacaaata gatgattttt
    27541 aatcatctat cttcctctca gttacatgaa taaaccacca aatatagcgc acccctataa
    27601 ttcccaatac tttattcatc aaacatttaa ttcataccat caattaatta cttttacatt
    27661 taataaaatt aataatattt ttaagaaaaa ctaaaaacat tcgacaatca aataagatat
    27721 tcttgcaccg atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa
    27781 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
    27841 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
    27901 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
    27961 tcacatcaca gcgatgccgt tgctgtcgac ctgctcttag tcaactgtga tctggaaaac
    28021 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
    28081 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
    28141 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
    28201 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
    28261 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
    28321 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
    28381 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
    28441 cttgcgcatt ggcggtcgaa ttcaatctga atgttctcga taaatcaatg cgcgaccgca
    28501 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
    28561 ttgcggtaaa taataaccaa atcagtaacg agcaaaagat taactggcac aaaaatgacg
    28621 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
    28681 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
    28741 tcttcaattt cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg
    28801 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtgtcc
    28861 tgatggatta caacttgttt gccagcagct accgcccaca ggatggcagc agcagcacta
    28921 atcttaacgc ctatggaaca gccggaatca acacgggggc atggcgctta cgcagtgatt
    28981 atcagttgaa tcatactgat agcgatgata accatgaaca gtcaggcgaa atatcacgta
    29041 cctatctttt tcgcccgtta ccgcaattag gttcaaaatt aaccctcggc gaaacggatt
    29101 ttagtcccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgatc
    29161 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
    29221 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag
    29281 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
    29341 cggaagaaga tggtcgggtg aacaatttcc aggtttcagc agcatcgacg cccttcctga
    29401 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
    29461 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
    29521 cctcgctgta cagcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta
    29581 ttgggcaaaa tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc
    29641 attttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
    29701 tggatgccac taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
    29761 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
    29821 agacgatcag cttatctgtg ggccaaccga ttaccccact aaacctcaat ctttacgcca
    29881 acctgctaca tcaaacctgg tggaatgcag acgcctccac gaccgccaac atcacggcag
    29941 gttttaatgt tgatattggt gactggagag atatctctat ttctacgtca ttcaatacaa
    30001 cccattacga agataaagat cgcgacaacc agatttacct gtcgatttcg ctccccttcg
    30061 gtaacggtgg tcgggttggc tatgacatgc aaaacagtag ccacagcacc acacaccgca
    30121 tgtcgtggaa cgatacgctg gatgaacgta atagctgggg catgtctgcc ggactgcaat
    30181 ccgaccgtcc tgacaatgga gcccaggtga gcggtaacta tcagcacctg agttcagcgg
    30241 gtgagtggga tatttctggt acctatgccg ccaatgatta cagttccgtc agcagcagct
    30301 ggagcggttc tttcaccgca acccaatatg gtgcagcgtt tcatcgccgc agctccacca
    30361 atgaaccacg cctgatggtc agcaccgatg gcgtggcaga tattccggtt cagggcaatc
    30421 tcgactacac caaccatttt ggcattgcgg tggtgccgtt gatttccagt taccagcctt
    30481 ccaccgtggc ggtgaacatg aatgacttac ccgacggcgt aacagttgca gaaaacgtta
    30541 tcaaagaaac gtggattgaa ggcgcgatag gttacaaatc actggcttcc cgttccggta
    30601 aagacgttaa cgtcatcatt cgcaacgcca gcggtcagtt tcctcccctc ggagcggata
    30661 tccgccagga tgacagcggc attagcgtgg ggatggttgg cgaggaagga catgcctggt
    30721 taagcggagt cgctgaaaat caaaagttta ccgtggtctg gggtgatagc cagcattgct
    30781 cgctccatct tcctgaacat atggaagaca ccgcaaatcg cctgatttta ccttgtcatt
    30841 aattaaggaa atgaaaatga catttatgaa aggactaccc cttttgttgt tggttgccag
    30901 tctgtgcagc cacgctgcac tacaacccga tcgcactcgt attgtgttta acgctaatga
    30961 taaagctacc agtctgcggg tggataatcg tagcgataaa ctcccctacc tcgcttattc
    31021 ctggctcgaa aatgaaaaag gggagaaaag cgatgatctt ctggtcgccc tgccgccgat
    31081 acaacgtctg gaaccgaaag caacaacaca ggtacggata gtaaagcaag cctcgaccac
    31141 aaaattaccg ggcgatcgcg aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc
    31201 cccggaaaaa aacagcgacc atgccgttct gcaagtcgcc attcagagtc gtatcaaggt
    31261 gttctggcga ccggctgcat tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca
    31321 ggtcagccag cagggcaacc aactgacact gaaaaatcct accgcgtatt atctgaccat
    31381 tgcctacctg ggacgaaatg aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc
    31441 gccttttagt acggtcaaca caaacaccgg aaattatagc ggcagccagt tttatctcgg
    31501 ttatatggat gattacggcg cgttacgcat gacgacgctc aactgcagcg gacaatgcca
    31561 tttacaagcc gtggaggcga agaaatgagt gctggcaagg gagtgttgct cgtcatctgt
    31621 ctgttatttc tgtcgttgaa gtctgcgctt gccctgaact gctactttgg ttcatccggt
    31681 ggctcggtag aaaaatcaga agcgattcaa ccttttgctg taccaggcaa tgccaaactc
    31741 ggcgataaga tctgggaatc tgacgatatt aaaataagat ctgggaatct gacgatatta
    31801 aaattcccgt ctattgtgac aacaatacca acggcaactt tgaaagcgaa catgtctacg
    31861 catgggtaaa cccctacccg ggggtgcagg atcgttatta tcaactcgga gtaacttaca
    31921 atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac cagtgtatcg
    31981 acagtaagaa cattgacatt tatacccctg atgggatggc agaacaaaat ttgctcgggt
    32041 gatcccgcta atattcacat gtcgcgaacc tttctcgctc gcatgcggtt atacgtcaaa
    32101 atacgagaaa tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa
    32161 tttgacggtg ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt
    32221 actggtctgg aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa
    32281 acacagatga ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg
    32341 aaaacattca gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa
    32401 gtcagttcct ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt
    32461 ggtaatggtc tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa
    32521 tatgctgaat acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct
    32581 gaactttcgt ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt
    32641 tttaaaatta actataactg atttgccatg atgccctttg cagccggaaa gggcatctct
    32701 ttcagaaaat tgctgatttt ccccttttcc ttgattgctg ttattttttc ctagcccggc
    32761 tttctttatg ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt
    32821 tttgcagtgg ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt
    32881 acatctcccg gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg
    32941 cggaattact ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg
    33001 catgattgcg caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat
    33061 cgtgcttgct attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt
    33121 gcgctatagc tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc
    33181 ggcaatggtc gctatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca
    33241 atatctgcga gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg
    33301 tgataacgct gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct
    33361 cctgcttacg attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc
    33421 ttccggcacg atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat
    33481 caccatcgaa ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat
    33541 tggtcttggt ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct
    33601 gctgtcgatt tttgctctgc tggctatttg tgcaggtatg gcgtggggac tgacccggtt
    33661 tatgcatatt gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt
    33721 ggcggtcatc gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct
    33781 gcgcctgttc agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc
    33841 gccgaagcgg tcggcctagt gctggcagcc tggacaccag taaaacgggc gcgaggacaa
    33901 cgtggttttc tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac
    33961 cttaaagcga aacagcgccc catgatgctt attctcatcc acctgccccc gcgtagcgta
    34021 agaaaaccga ggaatctcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag
    34081 atctttcgct ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata
    34141 attgccaagc ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg
    34201 aaagcgcggc gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac
    34261 atcgggacca acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc
    34321 aatatccgag gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa
    34381 tactcgcgtg gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg
    34441 attatggctg tagagcgtta aaccgttggg aaaatgagtt aacaacgcct taccacgcgt
    34501 ttccacatgg gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa
    34561 ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc
    34621 acggcggatc tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg
    34681 ggttatgctg cgacaacaat cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac
    34741 gccagagcgt gctcgccgtc gccatgcagg cagaccgttt gcgccgcgac cgttgcccat
    34801 tcgccggtga tacttttgac tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc
    34861 tgttcttcgt tttcaatcaa cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc
    34921 tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca
    34981 cgaatcagct cgcttcctgc cagcccaacg agaaccaatg ccggatcgca agcgtatacc
    35041 gctctggcga tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg
    35101 tgcggtttga catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca
    35161 atttgataca gcgtctgggc gtaaacggtt tccggcggca gctgcatggc gctgcgacca
    35221 aaattttccc tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct
    35281 tcacgcacgc aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca agcaatattg
    35341 gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg
    35401 ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt tcaaaataac gttgctgatc
    35461 ttgccgcgct ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag
    35521 cggaatttgc gccagatggt acatatcagc ctcaatgata caggcaatac gtggataacc
    35581 accggtggtc tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg gcacctgcac
    35641 cacgctcggt aacaaaccgt gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg
    35701 cccctgtaaa cgatagccca ttcggttact ttgcgagcta agctgccagg gcgaacgcca
    35761 gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat
    35821 gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt
    35881 gccaatcggg agtcagtcac catccttcag taaacggcct tccagcccgc caatccccac
    35941 tttgagatcg gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc
    36001 cagataactg cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat
    36061 cggcaatcgc cagccggtcc agacggcgtt atcatccaac cgcgcttcgc aacctgcacc
    36121 cgtcagggca aaccacccat cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc
    36181 cagcgcgggg gcattggcgt cattacccac cagcaggtta gcaatgcgca gcgcaggcat
    36241 atccagtgcg ccacagtggc tgatacccga ctggcaaaaa ccgtgacgac cgccatcctg
    36301 cacagtggtg tacatgcccg cacgaataat cttcagcata ttccctcctt ctgcggtaca
    36361 aatcgcacgc tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg
    36421 ctgagtgagg tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca
    36481 gtctgcggcc cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc
    36541 cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct
    36601 aaaaaccaga ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaac
    36661 ccgcaatacg ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga
    36721 atttcaataa agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa
    36781 cgctctatgg catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc
    36841 atgccgggaa tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag
    36901 atccgtttct ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca
    36961 cctatcagat aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg
    37021 attaggaata tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa
    37081 tgcgcgaata ccaccgcgtt cagtggcatg gtgacctgca gcataaaaat gcaatccctg
    37141 ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc
    37201 aaaacgcgcg gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg
    37261 gaccacctca ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat
    37321 ccaggaggct agttccattc ccggcacagg catggtcagc tctccccacg gcaccaacgg
    37381 ctcaatttcg cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag
    37441 ctcaggatgt gcgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa
    37501 cgtttttaaa cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta
    37561 gccatgatgc acaatgaccg catctgcgcc cagacgcact gcctcatcga gcaaagcctg
    37621 gctggcggtg acaccggtaa caattttttg caccgtctct ttgccttcca cctgcaaacc
    37681 attcggcgcg taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag
    37741 ttcggtgttt ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt
    37801 tacatcgcct gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca
    37861 aaagacgcaa attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca
    37921 gcatattgca taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac
    37981 gccgcgcaac tattacgtga cgttgccgtc aattgtggaa ttattatgaa taaacacgca
    38041 tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt
    38101 tacggcatgc gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact
    38161 cacgcctacg agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt
    38221 ggctttttgg cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg
    38281 atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg
    38341 tccctggcga ttatcgtctg cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc
    38401 ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg
    38461 gcgggtaacg tgggatctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac
    38521 agctgggcga tgggctttgg cctggcggcg gttggcatga tcgcgggtct ggtcattttc
    38581 ttatgtggca atcgtcattt cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg
    38641 acaaactttc tcctgccgaa ctggggatgg ctgctggttc tgctggtggc aacgcctgca
    38701 ctgattaccg tgctgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt
    38761 attggtctgg gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcggaag
    38821 gagctggggc tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa
    38881 cagggcggta gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt
    38941 tataccgttc cgaccgcgat gttccagtcg attaatgcct tcgcagttat gctgtgcggt
    39001 gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg
    39061 ggaaaatttg ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc
    39121 gcccgctggt ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg
    39181 gtgatgggct ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt
    39241 gaaattccgg gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc
    39301 gcgaactatc tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc
    39361 ggggcgatca actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc
    39421 gcactggcgt gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga
    39481 aaccgcgcgc tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga
    39541 cgcgtcttat caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc
    39601 tggattaggc gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat
    39661 ttccccttcc gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg
    39721 actatcggtt gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca
    39781 tgcaccgctt tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc
    39841 tcacggtcaa atttctcgcc ctgagttatc ggattgaaaa tacgaaaata cggcgcagca
    39901 tcagtacccg tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca
    39961 atcagctgcg acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc
    40021 agaaaactgg ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga
    40081 cgcatagcgg catcaataat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt
    40141 gcgggattgc tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac
    40201 aacgaggggt aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc
    40261 cagacactac cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa
    40321 cactggcgag gcgataaccc gcccgttgcc aagctggcgg acaaacggct ggtgccttcc
    40381 actgccggaa aatctcgttg ttgctcatac tctccagcac cgttctcgca aaactggcgt
    40441 aattgcgcaa tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga
    40501 ggataattca gcgtaatgga tggcgcgggc tttatcgatc cgctactacg aacttttggt
    40561 gcagcgacgc actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc
    40621 gtaaagactt tgtacatctc gtgattgccg gtcatcaccg cccccggcgg caggatcacg
    40681 ctgtcatcaa atccttcaca caccacgtta cgcagcgctc tttcaacgtc cacatcccgc
    40741 gctcgctcat tcacctcata ctgatagtta taaaaaagat gagtaacgct gttttccgcg
    40801 cacacctgtt taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa
    40861 ggaatacctt tttccgccag cgcgatttgt agcccattca gttgagcatt gataagttca
    40921 gcctgacgcg gcgacatgtt atgcgtctcc cactggcgtg gtgtagcgat atacagcgcc
    40981 agcacgcgtg cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt
    41041 aaatcctggc gaaaccaggc cagatgggta gtcataaaac tcctgaatgg cgcaagttaa
    41101 tcacacgcgg ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg
    41161 ccatatagtg tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac
    41221 tgtcgataag cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac
    41281 cctgaacgtg catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa
    41341 caattacgcg ttggcggtac tggttgtaaa aatcatccag attttgccac tgatgaatat
    41401 cagccacaaa cgggccaagt ttgcggtact cgggctgaga atgggccaga aagacgagtt
    41461 tatagcggct gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat
    41521 gtaactcatg aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgtagattca
    41581 ttactgctcc tgaacggtat acaggagtaa gtgtaggcgt tggtaaatga tgtttgtgaa
    41641 gttaatccgc tagcagggca agattacgaa agcgcgctcc ccgcaagcaa ctaatcaagg
    41701 tagttaatca tggctgaaca cgatattacg ccagaagtca ccatttccaa aacacagcgc
    41761 cgctataaag tcggctatgt cagagcccgc caccaggacc gcaaaaccgg tatagcccgg
    41821 tattattcac aacatcccag cctgcacctt aagggcgact ggctgaaaga ggccggattt
    41881 gataccggac ggggtgtcac ggtgaagatt tcggaggggg gcctgaccat catcgctgac
    41941 agtgacgagg tgcaggaact gcgggatggg atgtttagtg tgctgaacga aagttaaata
    42001 ttaaaaaagc tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg
    42061 atactcatca tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa
    42121 cctagttttt aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga
    42181 tggatcgtat tcattatcta tgtcagaagc taaaaatgat gatattcgta gtttaaattt
    42241 agcttcatct ttattacatt cattggcata aggtgaatgc cctatcatag catattcatc
    42301 taatctaaaa ggataagatg gttttaattc atcgatcaat gaaatttcaa tccatgttct
    42361 atatccaggc attaatggac taatttcttt gagcaaaacc tttccactta ataatgtatt
    42421 gagattgttc attattatgt ctcccacggg aaaaatatat gtctaacact tccaccatgt
    42481 tgatgaactt gtaaatgtgg taaatcacca tcaggatttc ctagtccatg acctgacact
    42541 ctatatgaag gttttgttac agaaggatct tttatatcat cccaatgata tgaacctgct
    42601 tttgttttat ttccagtaag atattccatt aagttcttag tgctggggcc tgactcacct
    42661 gtcatatttt tataattatg acctaaatat ctatccataa acaattgttc agcgtcactt
    42721 ctagacctaa cagtaacttt ggtttcacta ctatctaata aacgattcgc tttttggtta
    42781 ctaacagcac atggagttaa tccaagtgga tcagcatatt ttagaggatt aggtgcataa
    42841 ctgtagagat ttaaaccgcc tctcaaccct atcgggtcag ttaccatgaa ccgcccgaca
    42901 tccggatcat agtacctgtg cagattgtag tgcagccccg tttccctgtc gaggtactgt
    42961 ccctggtagc gcaggttctg ctccaccccg ccgtggtcct tctcctgtat cgtgttaccc
    43021 cagacctgat accccgtctc ccacacgatt ttcccgtcgc tgtccgtcat ctcttccggc
    43081 gcgccgttca catccgtgtg aaaatacagt atccggttcg ggccctcttt gcccgcctta
    43141 tccacccgcg ccagcggttc ataactcccc tggtccctgt agatgtacag gctgcctgtt
    43201 ccctccggcc tgctctcctg cgccagccgc attccgtccc ataaaaattt tgtccggttg
    43261 tacggcttac cgtcagggct gatgtgctgt ttttccgtcc gcctccccag tgcatcgtac
    43321 acataccggt atgtcccccg cgctgtcgtc acttccacca tccggtgttc cgcatcataa
    43381 cggtaactct gcgttccgct ccggccctcc ttttccaccg cacggccaaa ttcgtcataa
    43441 cggtagtgca caccacggta agacatcagc cggttgaacg gcaccacgct gttttctgcc
    43501 gtggcccttt cccccgcgcg cctccccagc aggtttcccg ccgcgtcgta ctgccagctc
    43561 gccagatacc cctcatcctc cgaacgacac gccgtgatgc gccccgtggg gtcgtagaag
    43621 taatccgttt ccccccgccg gctgtgccgc gtcttcagca gttcatcctc gccactgtag
    43681 ctgtactgcc ggtccgtgaa cgtctccggt accaccccgc gcatcccgct gtatgtctcc
    43741 ctgcgcttca gccgcccgcc ggggtcgtaa agctgccgcg tgctcagtcg cccctggctt
    43801 ctgagcactt cacggtgcag gtggtcgcgc gtgaactcgc tgatgacctc ccggtccaga
    43861 tttatctgct ggagatggcc gctgccgtag tacagatggc gctgcgttct gccgtccgga
    43921 aaggtggtgg cagtccggtt gcccagttta tcgtactggt gggtcaggat gccctgcgcc
    43981 gtctcctcgc tcagcatccg ccccagtctg tcgtagctga agcggataac ttccggcgcg
    44041 tcagtttcgt gccgctggcg acggatttcc agtagctggt ctgccgcatc atggacgtat
    44101 tccgtacggc catcggctgt ctcttttgcc gtgagccggc ccgccgcatc acgcgccagc
    44161 cggtggcgga tttcaccgcc ccggtccccg gtgaacacca cttccgtgac cgcgttcagc
    44221 gcgttgtagc ggtacgactg gcggcagccg tccgggttta tctgctccgc cagacggtcc
    44281 agcacatcat ggcggaaccg gtagcgttct ccgttctcgt tcgtgagcgt gaccagacgc
    44341 ccgtaagcgt cgtacccgaa tgccgtcctg cgccccatcg cgtccgtccg gctgcgcacc
    44401 cggccctgcc cgtcccgctg ccagcgcgtt atcctccccg tcgggctggt gtactttacc
    44461 agcctgcccg ccgcgtctga ctggtaattc accgtgcggc cgtccgccag tatcacctcc
    44521 gtcagatgac cgctgtcgtc atacccgtag cgcgtgctgt ggctctgcgc atccgtcatt
    44581 ctgataagcc tcgtgcgttc gtcgtagaac cacgccgtct gcgagccgga acagtccgtg
    44641 tgccgcatga gctgcccgtc ttcattccac tggaggtatt tattcccccc gcgcgcatcc
    44701 gttatctgca ccacctagcc gtggtggtca tactgatacc gggtgttctg ctgcagcggg
    44761 tcggtcacgg caagaaggtt gccgcgttca tcgtacacac agcgccaggt gtttcccgcc
    44821 gcgtccacct ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc
    44881 cccagcgggt cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg
    44941 ccgccctgag ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc
    45001 gtcacctgtc cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga
    45061 taacgtgtgc gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc
    45121 agccggtact gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc
    45181 ggaccttcat accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc
    45241 gtcatgcagt ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac
    45301 acgccgttca ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc
    45361 tcagggaaat gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac
    45421 agacggatgc acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg
    45481 tagtacagat tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc
    45541 gggtggtgcg ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag
    45601 tgcccctgct caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc
    45661 ggcagtacgg cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt
    45721 tcgtctgcgc tacccggtgt gcacacgagg ctgactttat acattacact ccagccctga
    45781 ccaaacattc cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga
    45841 aagtggcccg gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg
    45901 gcggcattga ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg
    45961 acactggcag caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc
    46021 gccacggttg ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt
    46081 aacggaccgc caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc
    46141 accctggcct cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc
    46201 acatcccgcg cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac
    46261 agattactga tgctgccaat aaccgcattc actccctgaa aggcggtttc cagcataccg
    46321 cccggattca gcaggttaag ggccgttccc agggggtcat ccagaaaggc ggcttttttt
    46381 tgtgcgagaa actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg
    46441 gtcggggtgg atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa
    46501 tgtgtcacgc catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga
    46561 gcccgggaca tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc
    46621 atttccagat attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct
    46681 tcttcctgtt ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca
    46741 gtcgccgctg cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg
    46801 atgtaacctt caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg
    46861 accttttctg tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg
    46921 acggccgtgc accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca
    46981 atgagggcat caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc
    47041 ataaacgacg tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg
    47101 tcccgttccc tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc
    47161 tttttaattt cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag
    47221 gtgaaggcca tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc
    47281 tgctcgttac cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt
    47341 ccgtcataat gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt
    47401 tttaatcatg aatgtaatac ccatcaccgc tttacagcac actttatctt tccagccata
    47461 atcactaata atattaagca attagcaata tttacaacga ccagagcgga tacatagtta
    47521 tgtatttaac aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct
    47581 accgcctcca tataaaaatg gacaccttat aaaattgtat cttgcataga aaaagctgaa
    47641 atatcggatg cgttttaatt atcaactata aatctataag ctttttatta acaaca
//
LOCUS       DADLCQ010000014        47405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000014 DADLCQ010000000
VERSION     DADLCQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.014"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            80..562
                     /gene="sseA"
                     /locus_tag="JQG95_000730"
                     /pseudo
     CDS             80..562
                     /gene="sseA"
                     /locus_tag="JQG95_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1383..2159)
                     /gene="sseB"
                     /locus_tag="JQG95_000731"
     CDS             complement(1383..2159)
                     /gene="sseB"
                     /locus_tag="JQG95_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY5887388.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2301..3584)
                     /gene="pepB"
                     /locus_tag="JQG95_000732"
     CDS             complement(2301..3584)
                     /gene="pepB"
                     /locus_tag="JQG95_000732"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY5887389.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3643..3843)
                     /gene="iscX"
                     /locus_tag="JQG95_000733"
     CDS             complement(3643..3843)
                     /gene="iscX"
                     /locus_tag="JQG95_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY5887390.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3855..4190)
                     /gene="fdx"
                     /locus_tag="JQG95_000734"
     CDS             complement(3855..4190)
                     /gene="fdx"
                     /locus_tag="JQG95_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY5887391.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4192..6042)
                     /gene="hscA"
                     /locus_tag="JQG95_000735"
     CDS             complement(4192..6042)
                     /gene="hscA"
                     /locus_tag="JQG95_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY5887392.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6059..6574)
                     /gene="hscB"
                     /locus_tag="JQG95_000736"
     CDS             complement(6059..6574)
                     /gene="hscB"
                     /locus_tag="JQG95_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY5887393.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6670..6993)
                     /gene="iscA"
                     /locus_tag="JQG95_000737"
     CDS             complement(6670..6993)
                     /gene="iscA"
                     /locus_tag="JQG95_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY5887394.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7010..7396)
                     /gene="iscU"
                     /locus_tag="JQG95_000738"
     CDS             complement(7010..7396)
                     /gene="iscU"
                     /locus_tag="JQG95_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY5887395.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7424..8638)
                     /gene="iscS"
                     /locus_tag="JQG95_000739"
     CDS             complement(7424..8638)
                     /gene="iscS"
                     /locus_tag="JQG95_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY5887396.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8750..9238)
                     /gene="iscR"
                     /locus_tag="JQG95_000740"
     CDS             complement(8750..9238)
                     /gene="iscR"
                     /locus_tag="JQG95_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY5887397.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9508..10248)
                     /gene="trmJ"
                     /locus_tag="JQG95_000741"
     CDS             complement(9508..10248)
                     /gene="trmJ"
                     /locus_tag="JQG95_000741"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY5887398.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10367..11170
                     /gene="suhB"
                     /locus_tag="JQG95_000742"
     CDS             10367..11170
                     /gene="suhB"
                     /locus_tag="JQG95_000742"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY5887399.1"
                     /translation="MHPMLNIAVRAARKAGNLVAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11315..12169
                     /locus_tag="JQG95_000743"
     CDS             11315..12169
                     /locus_tag="JQG95_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5887400.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12360..13640
                     /gene="csiE"
                     /locus_tag="JQG95_000744"
     CDS             12360..13640
                     /gene="csiE"
                     /locus_tag="JQG95_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY5887401.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13632..14771)
                     /locus_tag="JQG95_000745"
     CDS             complement(13632..14771)
                     /locus_tag="JQG95_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY5887402.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14931..15821)
                     /gene="hcaR"
                     /locus_tag="JQG95_000746"
     CDS             complement(14931..15821)
                     /gene="hcaR"
                     /locus_tag="JQG95_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY5887403.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15957..17318
                     /locus_tag="JQG95_000747"
     CDS             15957..17318
                     /locus_tag="JQG95_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY5887404.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17315..17833
                     /locus_tag="JQG95_000748"
     CDS             17315..17833
                     /locus_tag="JQG95_000748"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY5887405.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGIAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17833..18153
                     /locus_tag="JQG95_000749"
     CDS             17833..18153
                     /locus_tag="JQG95_000749"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY5887406.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18150..18962
                     /locus_tag="JQG95_000750"
     CDS             18150..18962
                     /locus_tag="JQG95_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY5887407.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18972..20174
                     /locus_tag="JQG95_000751"
     CDS             18972..20174
                     /locus_tag="JQG95_000751"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY5887408.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            20271..20693
                     /locus_tag="JQG95_000752"
     CDS             20271..20693
                     /locus_tag="JQG95_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY5887409.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20741..21613)
                     /locus_tag="JQG95_000753"
     CDS             complement(20741..21613)
                     /locus_tag="JQG95_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY5887410.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21625..22719)
                     /locus_tag="JQG95_000754"
     CDS             complement(21625..22719)
                     /locus_tag="JQG95_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY5887411.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22752..23750)
                     /locus_tag="JQG95_000755"
     CDS             complement(22752..23750)
                     /locus_tag="JQG95_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5887412.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23775..25286)
                     /locus_tag="JQG95_000756"
     CDS             complement(23775..25286)
                     /locus_tag="JQG95_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY5887413.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25309..26292)
                     /locus_tag="JQG95_000757"
     CDS             complement(25309..26292)
                     /locus_tag="JQG95_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY5887414.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(26389..29490)
                     /locus_tag="JQG95_000758"
     CDS             complement(26389..29490)
                     /locus_tag="JQG95_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY5887415.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFYPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            29788..30971
                     /locus_tag="JQG95_000759"
                     /pseudo
     CDS             29788..30971
                     /locus_tag="JQG95_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(31169..32422)
                     /locus_tag="JQG95_000760"
     CDS             complement(31169..32422)
                     /locus_tag="JQG95_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY5887416.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32749..33939
                     /gene="hmpA"
                     /locus_tag="JQG95_000761"
     CDS             32749..33939
                     /gene="hmpA"
                     /locus_tag="JQG95_000761"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY5887417.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33984..34322)
                     /gene="glnB"
                     /locus_tag="JQG95_000762"
     CDS             complement(33984..34322)
                     /gene="glnB"
                     /locus_tag="JQG95_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY5887418.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34383..35717)
                     /gene="glrR"
                     /locus_tag="JQG95_000763"
     CDS             complement(34383..35717)
                     /gene="glrR"
                     /locus_tag="JQG95_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY5887419.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35707..36420)
                     /gene="qseG"
                     /locus_tag="JQG95_000764"
     CDS             complement(35707..36420)
                     /gene="qseG"
                     /locus_tag="JQG95_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY5887420.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36585..38012)
                     /gene="qseE"
                     /locus_tag="JQG95_000765"
     CDS             complement(36585..38012)
                     /gene="qseE"
                     /locus_tag="JQG95_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY5887421.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFDPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38588..42475)
                     /gene="purL"
                     /locus_tag="JQG95_000766"
                     /gene_synonym="purI"
     CDS             complement(38588..42475)
                     /gene="purL"
                     /locus_tag="JQG95_000766"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY5887422.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     ASLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLSLGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42734..44290
                     /gene="mltF"
                     /locus_tag="JQG95_000767"
     CDS             42734..44290
                     /gene="mltF"
                     /locus_tag="JQG95_000767"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY5887423.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44287..44823)
                     /gene="tadA"
                     /locus_tag="JQG95_000768"
     CDS             complement(44287..44823)
                     /gene="tadA"
                     /locus_tag="JQG95_000768"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY5887424.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAVGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44848..45483)
                     /gene="yfhb"
                     /locus_tag="JQG95_000769"
     CDS             complement(44848..45483)
                     /gene="yfhb"
                     /locus_tag="JQG95_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY5887425.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45692..46540
                     /locus_tag="JQG95_000770"
     CDS             45692..46540
                     /locus_tag="JQG95_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY5887426.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46879..47370)
                     /locus_tag="JQG95_000771"
                     /pseudo
     CDS             complement(46879..47370)
                     /locus_tag="JQG95_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436854.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11305 a        12978 c        11923 g        11199 t
ORIGIN      
        1 catggacgac tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg
       61 ggtacgaata aagtaacggg gacttgcagg ctggcagcgc gatgacctgc tattagaaga
      121 aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg aagcagtggt
      181 gaaagtaacc gatgtgttat tggcaagcca tgaaaatacg gcgcaaatta ttgatgcccg
      241 cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac gtcgcggaca
      301 tattcccggt gcattgaacg ttccgtggac ggaactggtg cgcgaaggcg aactaaaaac
      361 gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctatgaca aaccgattat
      421 cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg cgacgctgga
      481 tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc gggcagattt
      541 accggttgag ccagtgaaat aagtatttta caggcaataa aaaaccgccg aatttggcgt
      601 ttttttattg ctggtctggt tcgcggcctt tccagcaggt tgacttgtgt tacacatgag
      661 caacgcaggt gcttcacagc aaaacaatac tcaccagtaa ctctcttttt gtcaagcaaa
      721 agagagtaat tattgtttat ttagcgtatt atcgacaccg gccctttccg ccgtgttcgg
      781 taataaaata acctggctta ttagtccgaa ttcagacaaa tataaacaaa tcctgctcaa
      841 aattaaaaat tctaaccggt aaaagatatt acttaaacat gtaaatttac tttcctttaa
      901 aaaacaaaaa accgccaaaa tcaggcggtt atttgttgct ggtccggttc gcggcctttc
      961 cagcaggttg tattaccgta gtaatgcaag cgcgtctcag cggagacaat actcgccagt
     1021 aactctcttt ttgtcaagca aaagagagtt attattgttc tgttagtgta ttatccactg
     1081 cggccctttc cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg
     1141 ttagagcaag cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg
     1201 atgcggtctt cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg tacttattgt
     1261 ttctggtgcg ctgttttccc aagtaaataa taaccggagt ctctccggcg acaatttact
     1321 ggtggttaac aaccttcaga gcagcaagta agcccgaatg ccgcccttta gggcggcata
     1381 ttttagatta tccggttttg tttaaagtca cgcaaaaaac caccccagcg acgttcatag
     1441 aatggcgcaa tatgttcggt aataaagtgg ctaattcctt tttccccttt tttcacctga
     1501 caaatatcga ttggttcatc gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga
     1561 ataatttctt cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct
     1621 tcgttctctt taattgaaca aataaaagca cgcttcaccg gcttaatggt tttaaataag
     1681 gtggtgagtg aatcaatcat ttgtgctggc ggctctgcga cttccgataa tatcagcgat
     1741 tcaccgcctt ccaggatttc ctggctgctc agcggatttc cctcttcacc aatcaacaaa
     1801 ctgatttcac gcggcataaa ttctttaccg gtcggcagtt tggcattaag gaagagcgtt
     1861 tcgccaaggg tcatctcaaa caatgtgcga acgggcatta cgacaaatgc ctgttcgtct
     1921 tcaaccgcct gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct
     1981 tctttttccc agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc
     2041 gccgtaccag gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc
     2101 gccggttcgg ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata
     2161 agaaccacaa ttcattcaac gttcggggcg caaatgcctg atgcgctacg cttatcaggc
     2221 ctacaaggga ttcgcaattt gttgaatttg cagaatttgt aggccggata aggcgtttac
     2281 gccgcatccg gcatattagt ttacgccgtt aacagattag ctatcgtgcg cacaccaagt
     2341 cccgtagcgc ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg
     2401 atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg
     2461 ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc
     2521 agctggctgc ggtggaattc cgccagcggc agacgccaga atggttcgtt ctcttgtgag
     2581 gcactcgcca gtaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca
     2641 ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat catttccggt
     2701 ttctgcgcac tggcatcaat cagaccatcg gcaagcacca gacgcccttc ggcatcagtg
     2761 ttcatcactt caactttttt accgttgcga taggtgatga tatcgcccag cttgaacgca
     2821 ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg
     2881 cgcataatgg caaatgccag cgccccggta accgttgccg cgccgcccat gtccgacttc
     2941 atcgagtcca taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct
     3001 ttacctacca ggcacgcgta caccggtgct tctttatcgc cagttgggtt gtaatccagc
     3061 gccagcaata ccggagaacg ttctgaaccg cgcccgacag tgtgcagccc catataacct
     3121 tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgcc cgcgacgttg
     3181 ctgatcagat caacagcacg ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg
     3241 ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctggcgctgg
     3301 gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcgggccttt ataaccttgc
     3361 cagaatgccc agcagcgatc cgcatcccag ccttcaccgc ttaactgaac atgcttgatg
     3421 cccagaccgt caatcttgcg ggccgcacgc tggatcagcc ccagatcgtc tgccccgttc
     3481 aaatgcaggg taatgccgtc attattaatg ctgtaagttg ctttttctcc ccagcgcgcg
     3541 tcggcaggtt gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttatt
     3601 cgtttaacag caaaacgggc tacccccagg cagcccgttc attcactcgg cttcgtccag
     3661 ccagactaac aaaatcgctt cgaggatttt ctcgttggat gcctgcgggt cgtcgtcgaa
     3721 atcttccaga tcgcaaatcc actgatgcat atcggtgaat cgaaccgttt tcggatcaag
     3781 atcgggatac gcatcgtaca gtgcttcgcc aatttcgcgg ctatcggtcc acttaagtcc
     3841 catactaacc tctgttaatg ctcacgcgca tggttgatag tgtaacgcgg gatttcgacc
     3901 actaaatctt cgtcggtgac gcgcgcctgg cagcttaaac ggctttccgg ctccagtccc
     3961 caggctttgt ccagcatgtc gtcttcctgc tctgagcttt ccggcagtga gtcaaaacct
     4021 tcacgaacga tgcagtggca ggtggtgcaa gcacaggatt tttcacaggc gtgttcaatc
     4081 tcaataccgt tacgcagcgc tgcgtcgaga atggtttcac cgctattagc ttccagaaca
     4141 gcgccatcag ggcagaggtc ctgatgaggc aaaataacaa tctttggcat attaaacctc
     4201 gtccacggaa tggcctttca gcgcacgacg aaccgactgg tccatgcggc gagcggcgaa
     4261 atcctgggtt tgtttgtcta cgtttttaat cgcttgttcg atggcgtcaa catcatcgcc
     4321 ctgcgccact tcactcaggt gagcggcagc attgtcaatg acctgacgct ctgcggcgct
     4381 taacagcgcg gcatcagcag ccagcgcgcc gtgcagactt tccagcacac gcgccgcttc
     4441 tactttttgt tctgccagca tacgggcttt tacgtcctgc tcggcatagc tcattgagtc
     4501 tttgatcatc gaagcgattt cgctatcggt cagaccgtaa gacggtttga cctgaataga
     4561 cgcctcaacg ccggtggatt tctccatcgc cgtcacgctc aaaagaccgt cggcatcgac
     4621 ctggaacgtc acgcgaatat gcgcaccgcc agccggtagc gccggaatac cacgcagcgc
     4681 aaaacgcgcc agtgagcggc agtcctgcac cagttcgcgc tcaccctgca ttacatggat
     4741 agacatcgcc gtctgaccat ctttaaaggt ggtgaaatcc tgagcgcggg ccaccggaat
     4801 agtggtatta cgcggaatca ctttctccac caggccgccc atcgtttcga ggcccagcga
     4861 cagtgggatc acatcaagca gcagcatttc gctgtctggc ttgttaccca ccagaatatc
     4921 cgcctgaatc gccgcgccaa tagcgacgac tttatccggg tcgatggaag tcagcggtgg
     4981 acgaccgaaa aattcgccta cccgttcacg caccagcggc acgcgagtag aaccgcccac
     5041 catcaccact tccagcactt cgtcagcttc tacacccgcg tctttcagcg cgcgacgaca
     5101 agccagtaag gttcgtttta ccagtggcgc gatcagttca ttgaattgtt cacggctgat
     5161 ttcgccctgc cagcccgcaa cgttaacggt cacggagtcc gcatcgctca gcgcgatttt
     5221 ggctgcaatg gtggcatcca gcagttcacg ctgaacgcgg ttatcgctac gatcaggaat
     5281 gtccgcctgc tcgcgaatgt aatccgccag cagatggtcg aaatcatcgc cgccgagcgc
     5341 ggaatcaccg ccggttgcca gcacttcaaa cacgccgcga cttaagcgca gaatggaaat
     5401 atcaaacgtc ccgccaccga ggtcataaac agcgatcacg ccttcctgac cggaatccag
     5461 cccgtaggcg atagccgcag cagtcggttc gttaagtaag cgcagaacat gcaaccccgc
     5521 cagacgcgcc gcgtctttgg tgccctgacg ctgggcatcg tcaaagtacg ccggaacggt
     5581 gataactaca ccatccagct cgcctgccag ggcttcagtt gcccgcgccg ccagtgcttt
     5641 gaggatgtcc gcagaaacgc gcaccgggtt cagtagcccc gccgccgttt caatcatcgg
     5701 caggccgttt tcgctggcct ggaattgata aggcagatgc ggatagcgtt gctggatatc
     5761 agccagcgag cgccccatca ggcgtttaac agaactaatg gtgttggcgg tgtcaagcgc
     5821 tgcgttagta cgcgcgtcat aacccaccga atgcccttgc tgttgatagt gaacaacaga
     5881 tggcagcagg tgacggcctt catggtcggc taacgtttcg gcctgaccgc tgcgcactgt
     5941 cgccaccagc gagttggttg tgcccaggtc aataccagcc gccagacgac gctgatgcgg
     6001 cgcagcactc aaaccaggtt cactaatttg taataaggcc atgtttagct tccagaaatt
     6061 aaaaatcgag cagtttttct tcgagttgtt cggcactgct ccgcagttta tcgagaaaac
     6121 gcagcttacg caccgtatcc gccgccgcgt cccacgcctc gttgtctaac tgttcaacca
     6181 tcaactgatg gcgggtatca aacatctttt tcacacgttt gataaagctt tccagccgcg
     6241 cttcatcttt cgcctgttcg atctcgtcca gctcttcgcg cagctccaac tgttccatca
     6301 ggaacgcggt gtcgcgcaca gtatgctgct cgctggcgag atcaaaaccg tgtaaagaaa
     6361 gcaaatattc cgcgcgcatt aacggatgac gcagcgtttg ccaggcctgg ttaatggttg
     6421 cagattgctg tacggcggcg agttgttccg ccaggcttcc gctggcgaat ttatcaggat
     6481 gatactgacg ttgtagatcc tgaaaacgca ggctcagtgc ctgggtatcg agttgataac
     6541 gggcaggcaa gccaaagagg gtgaagtaat ccataacatt ctcagggctg cgatccgccg
     6601 cgataacggc gaatcaggta aaacaaaccc cacgcgcagg cgaccacggt ggggttatcg
     6661 gtatgcgcat caaacgtgga agctttcgcc gcaaccacac tcatctttaa cgttcgggtt
     6721 ggtgaattta aacccttcat tcaggccttc ttttacgaag tccagctgcg taccgtccag
     6781 aaattgcagg cttttgccat cgaccacgac tttcacgcct ttgtcttcaa acacgatgtc
     6841 ttccggcgtc ggttcgtcaa caaattccag tacataagcc atacctgaac atccggaggt
     6901 tctcacgccc agacgcaggc caaacccttt gccgcggtta gccagaaagg tatttactcg
     6961 cgctgctgca ctgtcgctca gtgtaatcga cataaccaaa cctcaactct tattttgctt
     7021 cacgtttgct tttatagtcc gcaatggcgg ctttgatcgc gtcttctgcc agaatagaac
     7081 agtgaatttt caccggcggc agttcaagtt cttcagcaat atcggtgttt ttgatcgcct
     7141 gcgcttcgtc gagagacttc cctttcaccc attcggtgac cagggagctg gaagcgatag
     7201 cggaaccgca gccgtaagtt ttaaaacgcg cgtcttcaat gataccttca tcgttgactt
     7261 taatctgcaa cttcattacg tcgccacagg ccggtgcacc caccatgccg ctgccgacgt
     7321 tctcgtcgtt gttatcaaag gaacccacgt tacgcggatt ctcgtaatgg tcgataactt
     7381 tttcgctgta agccattata aattctcctg attccgatac cgattaatga tgagcccatt
     7441 cgatgctgtt cagatccacg ccctgcttgt acatttccca cagcggagaa aggtcacgca
     7501 gacgaccgat ggatttacga actaactcga tggtgtagtc gatctcttct tcagtagtaa
     7561 aacgacctaa agagaaacgg atagagctat gtgccagctc gtcgttcagc cccagcgcgc
     7621 gcagcacgta ggacggttcg aggcttgctg acgtacaggc ggaacctgaa gaaactgcga
     7681 ggtctttcag cgccataatc agcgactcac cttcaacgta gttgaagctg acgttgagaa
     7741 tgttcggcgc accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata tctttgatgc
     7801 cgttccacag acggttacgc aggccgcgca gacgttccat ctcggtcgcc atctcttctt
     7861 ttgcgatgcg ataggcttcg cccattccga cgatctggtg aacaggcaga gtgccggaac
     7921 gcataccgcg ctcgtgaccg ccgccgtgca tttgcgcttc gatgcgtaca cgcggtttac
     7981 gacgcacata cagcgcaccg atacctttcg ggccatagat tttgtgaccg gagaaagaca
     8041 tcaggtcaac tttcaactgg ctcaggtcga ttggcagttt gcccacgctc tgggttgcat
     8101 caacgtgata gataatgcca cgagcacggc acatttcgcc gatagccgcg atatcctgca
     8161 ccacgccgat ttcgttattt acgtgcatga tggaaacaag gatggtgtcg tcacgcatcg
     8221 ccgcttcgag ttctttcagg tcgataatac cgttacgctg cggtgccagg taggtgactt
     8281 cgaaaccttc gcgctccagc tgacggcaag tatccagtac cgctttgtgt tcggttttgc
     8341 tggtgatgat gtgcttgcct tttttctgat aaaagttggc tgcacctttg atcgccaggt
     8401 tgtcagattc ggttgcacca gaggtaaaga cgatttcacg cggatcagcg ccgaccagat
     8461 cggcaatctg attacgggcg atatctaccg cttcttcagc ctgccagccg aaacggtgag
     8521 aacgagaggc cgggttacca aaggttccgt ccatcgtcat gaactgcatc attttctcgg
     8581 caacacgcgg gtccaccggc gtggttgcgg agtagtcgag ataaatcggt aatttcattg
     8641 ctctataaac tccgtacatc actcaatgca aggaatcagg ctaccggctg gatgtacgac
     8701 cgtgtttacg gagtatttag cactccggcc tgattctgaa ttctttttat taagcgcgta
     8761 acttaacgtc gatcgcgtct tgtgtgcggg tgcgtggcgc gtcgtgagta tgctgacgac
     8821 cagacacatc cagcacttcc tggttattaa ccagttcgcc taaagtaatg ttgttgagaa
     8881 aaccggtgag acggtcgctc aaatcacgcc acagcgcgtg ggtcaggcat ttatcgccgc
     8941 cctggcagcc gcctttaccc tgacaacggg tggcatctac agattcgtca acggcgctaa
     9001 tcacttcgcc aacggcgatg ctgctggcat ctttgcctaa cagataacca ccgcctggtc
     9061 cacgtacgct ggaaaccaga ccatttttac gcagacggga aaacagttgt tccagataag
     9121 aaagggaaat tccctgacgt tcggaaatat cagccaacgg taccgggccc gcttcagagt
     9181 tgagcgcaac gtcaagcatt gcggtcacgg catagcgccc tttagatgtc agtctcatgt
     9241 cttacttcac ctcaaactcg cccctgcccg gggtttttta ttgtaaaagt gggggtattg
     9301 catagcaggg tcaagtctga cattcccgag taaattggtc aactatttac ttgactgatt
     9361 tagtcgggta tttaaccttc agtgccattt ttttatcgtg gcgtttgtac ctgttgtcgg
     9421 atgcggtgtg aacaccttat ccgacctacg attctgtaca ttgtaggcct gataagacgc
     9481 gttaagcgtc gcatcaggca accactgtta ttcggccttg ttacctttat tctgctgctc
     9541 aatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt
     9601 aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga taaaaccggt
     9661 tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga ccagcggata
     9721 cggcgtctct tcatgctcga cctgctcgcc attttcctga gttgccagcc aggccatacg
     9781 cacttcatag gcaataacct gaaccgccat tgccaggttc agcgaactgt attccgggtt
     9841 tgccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca agccgacgcg
     9901 ctcacgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc ttttcagacc
     9961 gcattcgcgc gggtcgagca tcggccacgg cagcgtgcgg gagcgtgcgc tggtgcccac
    10021 caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat
    10081 cacatcgctg gcccctgcag ccagggcaat cgcctgggag tcgggtttca ccagtggatt
    10141 aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt
    10201 gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttca
    10261 tctaaagatt attcacgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat
    10321 actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc atccgatgct
    10381 gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta gttgccaaaa actatgaaac
    10441 cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca acgtagataa
    10501 agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat
    10561 caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc
    10621 actggatggt actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt
    10681 tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt
    10741 caccgccact cgcggtcaag gcgcacagct gaacggctac cgtctgcgcg gtagcaccgc
    10801 tcgcgatctc gacggtacca ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc
    10861 cactacctac atcaacatcg tcggcaagct gttcaacgaa tgtgcagact tccgtcgtac
    10921 cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga
    10981 aatcggtctg cgtccgtggg atttcgcggc aggcgagctg ctggttcgtg aagcgggcgg
    11041 catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg
    11101 taacccgcgt gttgtgaaag ccatactggc gaacatgcgt gacgagttaa gcgacgctct
    11161 gaagcattaa tgactcaggc gggtgatatc actcacccgc cctcgccttt caggcgctat
    11221 tccgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttccccga
    11281 ttaatggatg ataaagaagt cgcaaggaaa ggttatggca ctgccagtga acaaacgcgt
    11341 tcccaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt
    11401 tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc cctggtcggc
    11461 ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt ttattccttc
    11521 ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg
    11581 aaatatgtcc gcccactggc cgctggtcag ttggttacct gagcgtaatt tcaacgtttt
    11641 tatgtttgat tatcgcgggt ttggtaaatc aaaaggcact ccgtcccagg ccggattgct
    11701 ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaaca
    11761 gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta ttggtcaggg
    11821 tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt atgcaaccat
    11881 cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa
    11941 ttacatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag ccgatcacgt
    12001 tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat
    12061 cctaatcccg gatggcgaac acattgatgc tttttccgat cgtcacggca atgtttatcg
    12121 cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac ccatgatcgc
    12181 tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg tcagaacagt
    12241 ttaaaatgag aaaaattctg tgacgcttgc caacatttct gatgattagc attcccttcg
    12301 ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga
    12361 tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct
    12421 tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc gagcttaatg
    12481 gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag atcctgcgct
    12541 atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa ggtacagtgc
    12601 ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccgtcat
    12661 ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc
    12721 gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacggctgc
    12781 aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa tattgtttgc
    12841 tgcagcacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat
    12901 cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc
    12961 agcatgtacc gcctgatgag ccactgttta tggcattact tttttccatg ttgcgggttc
    13021 ccgatccact gcgggatgcg catcagcggg acagacaatt gcgtcagtct atcaaacgtc
    13081 tggtaaacca ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag gggttatgtg
    13141 atcagcttta tatccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg
    13201 ataataccct gccggaagag ttcgccagac tgtatccacg cctggtacgc accacccgcg
    13261 cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagtggtc
    13321 tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat gaaaaacaga
    13381 ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag cagttacgcg
    13441 aactaacgtt actgccgctc aacattaagc atatgtcggt aaaggcattt ttgcagacag
    13501 gcgctccgcg tggcgcggca ctgattattg cgccttatac catgccgtta ccgctctttt
    13561 caccaccgct gatctatacg gacctgacgc tgacaacaca tcaacaggag cagatccgca
    13621 aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc cggaagcgcc
    13681 accagcgcca ttacccagaa cacgccgtgg cccagatatt gatacaggaa accggcgaaa
    13741 acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg
    13801 atgacttcgc taacctggcg agcagcaata taacgcatgg cggccaggtg gcagaccgtg
    13861 aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt agttgctccc
    13921 ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc gcgagcacta
    13981 caacggcgga aaagtttatt actcagcgca aagataatga cttccgccac cacgcccagc
    14041 gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta
    14101 aaaccgtaat aggccgcatg tgccccctgc aataaacaaa cgcaggccag aaagcgccag
    14161 ttctggcgaa ccagcgccaa ccacgcagac caaccggtgc tctcctgctg gcggcttgcc
    14221 ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac
    14281 gtcaacagca cgagaatcac ccgataatca aacatagtga ccagtttgcc cgtcagcgcc
    14341 gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc aagcgggaac
    14401 tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg
    14461 ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca gaaggcgaca
    14521 gcaaagagaa gtggcagcag tgccagcacg cgcaaggcgg aaatcaggcg ggaaggatcg
    14581 ctgacgcggg gcgcgatgag caaactcccg agaaaacggg caaccagacc tgcccccaat
    14641 aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt
    14701 aaaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat
    14761 tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggggttaatc gaatgttggt
    14821 gcaagttgaa aaataagaat attcgagcct gcttcccgat gtctgtaacg ccagttcaca
    14881 agcactaaaa aactgcgatc cgcgacgcat gttgcaaaaa taatgcgtaa ttatgccgtt
    14941 acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccag cttcatctcc
    15001 cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat gggacgaaaa
    15061 acaacctgcc cggtattaaa attattcata taaccgggta tcaaagtgac gcccagcccc
    15121 atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat atttggctgg
    15181 ctgttttctt gcgcaaacca cgctttaacg atcggcgcaa gcgaaccgga atacgccgga
    15241 tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt ttcatgtgct
    15301 aaagggtgat caaccggtaa caccaccact aatggttcgt caaaaagctc cagataatca
    15361 atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc acggcgaatt
    15421 ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc tggctgtctg
    15481 agacgaaaca tcggtaatac ttttggcaat aaattcactt ccgccgatgg cacaaagcca
    15541 atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc attttccgct
    15601 tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgccgt caacgcgact
    15661 ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc gcggatctgg
    15721 ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa gttcagtgcc
    15781 tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc cttgttatcg
    15841 aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca accagcaaca
    15901 tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta aaaaatatga
    15961 ccacaccctc agatttgaac atttaccaac tgattgacac ccaaaacggt cgggtcactc
    16021 cgcgtattta taccgacccg gacatttacc aactggagct tgagcgtatt ttcggtcgtt
    16081 gctggctatt tctcgcccac gaaagccaga tcccaaaacc cggtgatttc tttaacacct
    16141 acatgggaga agatgcagtt gtcgtggtgc gccagaaaga cggcagcatc aaggcgtttc
    16201 tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgtggc aacactcgcg
    16261 cctttacctg cccgtatcac ggctggtctt atggcattaa cggcgagttg atcgatgtac
    16321 cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactggggg ctaaacgaag
    16381 ttccttgtgt ggagagttat aaaggactga tttttggcaa ctgggatacc agcgcaccgg
    16441 gcctgcatga ttacctgggt gacattgcct ggtatctgga tggcatgctg gatcgtcgcg
    16501 aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgt aactggaaat
    16561 tcccggcaga gcagttcgcc agtgaccagt atcatgctct gttcagccat gcttctgccg
    16621 ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat ggacaaaccg
    16681 cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac ggtcacggta
    16741 gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc gcagtttcaa
    16801 gctattaccg cgaaacctat gccgaagcag aacaacgttt aggtgaagtt cgcgccctgc
    16861 gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc actgccacac
    16921 tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc tgtattactg
    16981 acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact cgtgcttttg
    17041 gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc cagaaattgc
    17101 ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt ggtcaggaaa
    17161 agcgccgcga cgacggcatt cctggcatta ctaactatat cttttcagaa actgccgctc
    17221 gtggaatgta ccaacgctgg gccgatcttc tgagtagcga aagctggcag gaagtgctcg
    17281 ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt cactggagtt
    17341 acatcaccgc attagccagt ttctctttca cgaagccagt ctgctggatg actggaaatt
    17401 tcgtgactgg ttggcacagc tcgacgaaga gattcgttac accatgcgca ccacggttaa
    17461 cgcgcaaaca cgcgaccgtc gcaaaggcgt ccagccgccg acgacctgga ttttcaatga
    17521 caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcattg cctgggcaga
    17581 agagccgccg tcacgcactc gtcacttaat cagcaactgc caggtaagcg aaaccgacat
    17641 cccaaacgta tttgccgtga gggtgaatta tctactttat cgggcacaaa aagagcgcga
    17701 tgaaacattc tatgttggaa cgcgtttcga caaagttcgc cgtctggaag atgacaactg
    17761 gcgcttgctg gaacgggata tcgtcctgga tcaagcggtg atcacttccc ataacctgag
    17821 tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc ggagggtgag
    17881 gctctccgga tagatacctc gcccgtcatc gccttgttca acgtgggcgg cgagttttat
    17941 gccattaacg atcgttgcag ccatggtaat gcgtcgatgt cagaagggta tctggaagat
    18001 gacgccacgg tggagtgccc gctacacgcc gccagttttt gcctgaaaac ggggaaagcg
    18061 ttatgcctgc ccgccaccga tccgctcacc acttatccag tacacgttga aggtggtgac
    18121 attttcatcg acttaccgga ggcgcagcca tgagcgatct gcataacgag tccattttta
    18181 ttaccggcgg cggatcggga ttagggctgg cgctggtcga gcgatttatc gaagaaggcg
    18241 cgcaagttgc cacgctggaa ctgtcggcgg caaaagtcgc cagtctgcgt cagcgatttg
    18301 gcgaacatat tctggcggtg gaaggtaacg tgacctgtta tgccgattat caacgcgcgg
    18361 tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat gcaggcatct
    18421 gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc ggcttccacg
    18481 agctgtttaa cgtcaacgta ctcggttacc tgctgggcgc aaaagcctgc gctccggcgt
    18541 taatcgccag tgaaggcagc atgattttca cactgtcaaa tgccgcctgg tatcctggcg
    18601 gcggtggccc gctgtacacc accagtaaac atgccgcaac cggacttatt cgccaactgg
    18661 cttatgaact ggcaccgaaa gtgcgggtga atggcgtcgg cccgtgtggt atggccagcg
    18721 acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc gataatgcag tctctgacgc
    18781 cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggcg gattttacgg
    18841 ggccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt gtgatgatca
    18901 acgctgatgc gggtttagcg attcgcggca ttcgccacgt agcggctggg ctggatcttt
    18961 aaggaagcac gatgaaagaa aaaacgatca ttattgtcgg tggcgggcaa gcggcggcaa
    19021 tggctgcggc ctcgctacgc cagcaagggt tcaccggtga gctgcatctg ttttccgatg
    19081 agcgacatct tccttatgaa cgacctccac tctcgaaatc catgttgctg gaagattccc
    19141 cgcagttaca gcaggtgtta cccgctaact ggtggcagga aaacaatgtt catctgcatt
    19201 ccggtgtaac catcaaaacg ctgggtcgcg acacacgaga gttagtgtta accaacggcg
    19261 aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga ccgctgccgt
    19321 tgcttgatgc actgggagaa cgctgcttta ccctacacca tgccggtgat gccgccagac
    19381 tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga actattggtc
    19441 tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt gaactggcgg
    19501 caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc
    19561 agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa
    19621 aagtagaact gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta
    19681 ttggtatcag cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca
    19741 ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg
    19801 caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata
    19861 accaggcgca aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc
    19921 cgtggttctg gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg
    19981 atgactggct ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa
    20041 acggcgtgct tatcggtgcg gtcacgctga atcagggccg tgagattcgc ccaattcgca
    20101 aatggatcca gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc
    20161 ttaaatcact gtaatcagga taattagcga atatctcaat gcctggggcg tggcgaggtg
    20221 caagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac
    20281 tacgttattt tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg
    20341 tggtcttaat tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc
    20401 aatatatggc ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta
    20461 tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc
    20521 tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg
    20581 gcgatgcggt tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt
    20641 tcttgctatt ggcaattacc ggaccggggg caatttctct cgatcggcgt taggaaagat
    20701 gccggatgcg gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca
    20761 acgacatctc tgaggttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg
    20821 ggcgatgatg atcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat
    20881 atcctttatc aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg
    20941 tttccatgat gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc
    21001 cggcaaaacc attgttcacc cactgacgcg gcaacggcgc gagctggtta aaatccagtt
    21061 cctgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgttcc agccagtaac
    21121 cgctcgcctg cgcctgaatc cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg
    21181 taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat
    21241 ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat
    21301 acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat
    21361 cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct
    21421 gccagacaaa acggttgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg
    21481 catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga
    21541 agccttcgat tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg
    21601 tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt
    21661 gccgctcgcc atcagcacat aggcatcacc tgcctgttct agcgagaagc gatgggtaat
    21721 ggcgttacgc ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa
    21781 cagactggtc acccaggagc cgataatccg ccgttgatgg tgcatcagat cagcgctgac
    21841 ctcgaattcc acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcagtgga
    21901 ttgcagtgcc agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc
    21961 accgtgggta agttcagcaa ttatctgcgg cagaccttcg gtggttgcta aatagccgtg
    22021 atccatcccc cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat
    22081 gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag
    22141 accgaccacc agcacgttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc
    22201 cgtgccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag
    22261 gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc
    22321 gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca
    22381 aaaaccacag ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca
    22441 gccttgcccc atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta
    22501 taacggttta tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc
    22561 gcttccgcaa atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg
    22621 caccgcaact tcccgcagat cgacggttga atttcctagt aaataagcag ccagcatcgt
    22681 tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac ccgcgccagg
    22741 ggcgttaagg tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc
    22801 accgccacca cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc
    22861 agcaccagac cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg
    22921 gaaccgcgac cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac
    22981 tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg
    23041 gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta
    23101 ataccgcaca actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag
    23161 gcggtttttc ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat
    23221 actggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc
    23281 actggcagtg cgttggtcat aaacagcccc attccgcgca gagcgctcca cagccccagt
    23341 gtggcaacga aacttggtac gttaaacacg ccgcgcagca ccccggcgag cgttcccatc
    23401 agcgcgccta acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc
    23461 aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca
    23521 ccggagataa taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc
    23581 agcacgttca tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga
    23641 taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt
    23701 ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcatacat cttcaggttc
    23761 ctcggtaaaa ttcatcagtg tacagacaga atggcggaca tcagctcatc cacattgacc
    23821 ggcgagtgaa actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc
    23881 agcggcaatt cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc
    23941 tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg
    24001 tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga
    24061 ttgccaccag aaagcgtgcc gatgggtgtt tcgctactgg cagccttgac cgtcatccgc
    24121 tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggtgctg
    24181 attttttgcc gattggtcag cactgtattt tcgtcaacgc ctagccaggg aatgatcccc
    24241 gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg
    24301 gggcgcatga ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca
    24361 acaatcgcct tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact
    24421 tcgccacgac gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg
    24481 acttccagta cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga
    24541 tcacgcccta gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg
    24601 cccgccacct gaccatcgcg cataacggtg gcataggagg caatgcggcg gatttcttcc
    24661 atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt caccgcactg
    24721 atcaccagtt caacttctgc actggcaagc gagctggtgg gttcatcaag aatgaccacg
    24781 cgcggctcgc ccttcatcac ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc
    24841 gttgaaacaa gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca
    24901 tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc gaggccattg accaaggcag
    24961 aggttttctg ccaccgtcag cccttccacc agacttaatt cctgataaac cgcacgaacc
    25021 cccagttcag cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca
    25081 atccagatat caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat
    25141 ttgcccgcgc cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta
    25201 acgttatcca gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc
    25261 gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc
    25321 atcaacgtgc gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg
    25381 cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa
    25441 aacagcattg cccattttct taccggaaat atccactacc gctttcagca cctgattgtt
    25501 ttccagctcc tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc
    25561 ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat
    25621 aatggcgttg agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc
    25681 taaaacagtc ccttcctggt tagcgacaat ttgcgcgccg ggaacgcggg attttaatac
    25741 ttcttcaaat cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc
    25801 aattttcggc tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt
    25861 tttaccaaat tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt
    25921 ttgattaata caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac
    25981 ggtacggcta ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc
    26041 cacgagggta tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat
    26101 taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc
    26161 gtaatatccc tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct
    26221 ggcaaatagt gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat
    26281 ttttttaggc atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt
    26341 tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta
    26401 gttaaaaata aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat
    26461 cgccgtcaat tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc
    26521 cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga
    26581 taacaggtca ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc
    26641 ttgcgcccac tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag
    26701 tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg
    26761 gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc
    26821 atcgccctgc gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc
    26881 cggtaaacgg ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct
    26941 ggcctgctgc ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat
    27001 aaactgactg gtgaccttcc cttccccgcc ttcccacggg tagaatttac gcgtggcgag
    27061 aatgtccgcc gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt
    27121 catgtcgtcg cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc
    27181 cggtgtggct ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg
    27241 cggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt
    27301 attccacgca tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac
    27361 gcaacgttgc cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc
    27421 cagtaaatgg cgagcaaacc agcactcttc aatactctcc agcgccgcca cttcttccag
    27481 cgtattcggg aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc
    27541 acgttcggct ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc
    27601 cagtgcgttc agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc
    27661 ggtcatcaga gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga
    27721 ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg
    27781 cagcagtttc tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag
    27841 attatgcaag caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa
    27901 atccagaccc gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc
    27961 gcctgcttta ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata
    28021 ttgtccctga cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcgggtt
    28081 tttgttcagc gcatgtgcgc gtttcagcgc ctgactggca tacgccaccg cttgcgggaa
    28141 atctgcgcgg ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc
    28201 cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc
    28261 cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg
    28321 cgctttggcg acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac
    28381 aatattgccg tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa
    28441 cacgccctgg atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca
    28501 tttgccgatt tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc
    28561 ccactcaatc ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg
    28621 caccatgccc agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc
    28681 atcaagccag gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata
    28741 cgggccgtta ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca
    28801 actccactgt tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc
    28861 ttcatcgtga caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt
    28921 tggaacaggc acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta
    28981 gtaagtgccg gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc
    29041 agtcacatcc ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttggc
    29101 ccaccacaag aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag
    29161 tgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc
    29221 cgtttcgccg acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac
    29281 gggcataaag gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat
    29341 ccacggtccc agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc
    29401 gcgctgtttc actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat
    29461 cactttgatg tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc
    29521 gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg
    29581 attttccagg aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac
    29641 gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga
    29701 attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg
    29761 caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattctc
    29821 caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca
    29881 acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg
    29941 gaaagcgaaa aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc
    30001 ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc
    30061 agtattgagt gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta
    30121 cagattgatg taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc
    30181 caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt
    30241 gatccggtga ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag
    30301 gtgaagtatc tgctggaggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30361 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30421 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30481 gcggacagat tggtcacacc atcgttaatc cggatggcgt cgtctgcgac tgtggacgtt
    30541 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30601 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30661 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30721 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30781 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30841 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30901 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30961 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    31021 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31081 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31141 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31201 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31261 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31321 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31381 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31441 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31501 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31561 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31621 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31681 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31741 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31801 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31861 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31921 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31981 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    32041 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32101 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32161 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32221 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32281 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32341 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32401 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32461 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32521 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32581 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32641 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32701 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32761 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32821 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32881 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32941 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    33001 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33061 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33121 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33181 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33241 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33301 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33361 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33421 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33481 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33541 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33601 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33661 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33721 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33781 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33841 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33901 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33961 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    34021 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34081 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34141 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34201 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34261 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34321 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34381 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34441 gtccggttgc gccccgccat tctagccgcg tgggtgacgt tgcctttggt gatttgcagc
    34501 agcttacgca aatagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34561 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34621 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34681 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34741 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34801 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34861 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34921 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34981 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    35041 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35101 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35161 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35221 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35281 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35341 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35401 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35461 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35521 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35581 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35641 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35701 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35761 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35821 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35881 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35941 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    36001 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36061 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36121 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36181 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36241 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36301 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36361 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36421 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36481 acagcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36541 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36601 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36661 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36721 accgcccctt ttcgctggtg gcttccctga aaaaaggggt cgaagatcat ggcgcgttcc
    36781 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36841 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36901 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36961 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    37021 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37081 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37141 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37201 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37261 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37321 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37381 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37441 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37501 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37561 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37621 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37681 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37741 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37801 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37861 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37921 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37981 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    38041 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38101 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38161 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38221 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38281 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38341 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38401 cttattagat tacaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38461 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38521 gttggtgctt aatgccggat gcggcgtaaa tgccgcatcc gggctgcaaa accaatgaac
    38581 tgatgactta ccccaactgc ttacgcgcat tgcggaaaat gcgcatccat gggccatcct
    38641 cgccccagtt ttccggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg
    38701 gcatcataat ggtgactcga ccactttcag tcgtaaccgc cgtaataccg ttcggtgaac
    38761 cgttcgggtt agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg
    38821 ccaccagccc tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc
    38881 cttcaccatg agagacagca atcggcattt gcgagcccac catcccctgc aacagcagag
    38941 acgggctttg ggttacttca accaggctga aacgcgcttc aaagcgatcg gaggtgttac
    39001 gcacaaaacg tggccacaac tcactccccg ggatcagttc acgcaggtta gacatcatct
    39061 ggcaaccgtt acataccccc agggacagcg tttgcggacg atggaagaag gttgcaaact
    39121 catcgcgtac acggtcattg aacaggatag acttcgccca accttcaccg gcacccagca
    39181 catcaccgta ggagaaacct ccgcacgcga ccagggcgtg aaaatcctcc agtcccgtac
    39241 gtccagtcag caggtcgctc atatgcacgt cgatagcatc aaagccccca cgttggaaag
    39301 ctgccgccat ttcaacatgc gagttcacgc cctgctcacg cagtacggca actttcggac
    39361 gtgcgccagt ggcaatatac ggtgctgcca catcttcgtt gatatcgaac gacagtttta
    39421 cattcaggcc cggatcggcg tcgttagatt tcgcctgatg ctcctgatcg gcacactccg
    39481 ggttgtcacg caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtag
    39541 tgcggctttc gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa
    39601 ccgcctgccc aacataatgg acgcaatccg caagcccatg ctgtgccagt acggactcga
    39661 ccgcttcacg gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg ttaaacaacg
    39721 ccgccaggcg atcgtcaccc agagaggcga tatccgcgtc aatgccacaa tgacctgcaa
    39781 aggccatttc cgccagcgtt accagcaggc ctccatcaga gcggtcgtga tacgccagca
    39841 gcttacgctg tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tggacaacat
    39901 cgcgtacatc tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc
    39961 ccagcgcgtt attgcctttg cccaaatcaa tcagcagcag tgcgttatct tcggtacaaa
    40021 gctgcggcgt gatggtgtga cgtacatctt ccacgcgggc aaatgcagaa atcaccagcg
    40081 acagcggcga cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca
    40141 tagagtcttt acccaccggg atcgtcaggc ccagcgccgg acaaagttct tcacccacgg
    40201 ctttaacggc ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt
    40261 tggcggaaag tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata ttggttaacg
    40321 cttcaccgac cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct
    40381 cgccaatcgc catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt
    40441 tagcgaccgg cacctgccac ggccccacca tctgatcgcg cgccaccatg ccggttacgc
    40501 tgcggtcacc gatggtcacc aggaaagttt tctccgccac ggtcggcaga tgcagtacgc
    40561 gtttcaccgc gtctgcaatg gtgatccctt cacggaccag cgcatcgccc ttcgctttca
    40621 gcgtttgtac atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat
    40681 cgatcggctg attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt
    40741 caccaatcac cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt
    40801 gatcggcagc aaccgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg
    40861 ggctcatgcc cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgtccgc
    40921 cgtcgctcac cagctccggc atcgcgttag aaagaccgcc cgcaccgacg tcgtggataa
    40981 acagaattgg gttagcatca ccaagctgcc agcaacggtc gatcacttcc tggcaacgac
    41041 gctccatctc cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac
    41101 cagacgccat ggaagacgcc gcgccgccgc caagaccgat gttcattgcc gggccgccga
    41161 gaacgaccag cttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt
    41221 tgccgatccc gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccattgt
    41281 ggctgttcac tttttcttca taagtacgga agtagccgtt cagtgccgga cgaccaaatt
    41341 cgttgttaaa cgccgcaccg cccagcgggc cttcggtcat gatgtccagc gcagtaacaa
    41401 tgcgctcagg cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt
    41461 tggatacgga gaaaccaacc aaaccagctt tcggcttcgc gccgcgcccg gtggcacctt
    41521 catcgcggat ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg
    41581 ggtggttgtg agtttcgact ttcatcagga tgtgcgccgg ttcctgatgg aaattgtagc
    41641 ggcccgtttc gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt
    41701 tatctttata agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatcc
    41761 tgaacagcga tttcggctgt tgttcaccgt cgataatcca gtcggcgtta aaaattttgt
    41821 ggcggcagtg ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac
    41881 gaccaagctt tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaagc
    41941 caagacgcag gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgcttg
    42001 tgaccggagt cggttgatgg tgggcaaaca actgctctgc atcatctaaa tcaaaaaaga
    42061 ccgtttccat catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca
    42121 gcgtaccggc ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc
    42181 cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg
    42241 gggtcaccag caggagtttg ccttgcgggg cgtggctggc gagtgccgga ccatatttca
    42301 gcaggcgttc aagttgtgcg tgctcatcat cgtttaacga cgcattgagg tcagcaaaat
    42361 ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca
    42421 gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc
    42481 tctcgtcttc taagctttcg gtgtacccaa ggggggggaa acgggcgtca ttataaagaa
    42541 tctgatgcgc tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg
    42601 cgtgccgcca atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc
    42661 atttatgagt aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca
    42721 gagaattaac taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact
    42781 gctgctcgcg gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat
    42841 cgccgccatt caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta
    42901 taacgaaatc aacgggaaac cttttggcct agattacgaa ctggcgaaac agtttgccga
    42961 ttacctcggc gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga
    43021 ccttgataat ggtaacgccg acctgctggc ggcaggactg gtctataaca gtgagcgggt
    43081 aaaaaattat cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt
    43141 gggtcagtat cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc
    43201 gggtcatgtg gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag
    43261 ctggaaggta gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa
    43321 actcgattac accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga
    43381 gctcgccgta gcgctcgata tcaccgatga acaaccggtg acttggttta gcccgttaga
    43441 tggcgataat accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg
    43501 tacgctggca cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga
    43561 tacgcgcaca tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga
    43621 gaaatacgcc gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca
    43681 ctgggatgca caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa
    43741 taccgcgcaa agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg
    43801 cgtgcgttat ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgaacg
    43861 gatctggttt gccctcgctg cgtacaatat gggctatgcg catatgctgg atgcccgcgc
    43921 cctgacggca aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc
    43981 tttacttagc cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga
    44041 agcctacgct tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca
    44101 agagaaagag aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt
    44161 atcgcctacg gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc
    44221 atcaaactat ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa
    44281 acaaaattaa tccgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat
    44341 gcgaaagaag tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc
    44401 cactcggtga ttcatacccg gatgatgcag cacatccatt aaagatccca cagcgccagt
    44461 tttcgcgtca cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc
    44521 acacattaca catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg
    44581 catcaccaga ccaccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatggcg
    44641 accaatcggg cggttccagc cttcgccgat tacccgattg ttatgcacta ataccgcgcc
    44701 gaccggcact tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca
    44761 atattcgtgg ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg
    44821 cattatacac ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt
    44881 tactcgccaa cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata
    44941 gccactgtac agccgcagcg gcgtgccgat tttgcgctcc agttgtgcga ccttttcatg
    45001 tcccagacaa cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat
    45061 aagattaacc cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg
    45121 agagccggta atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc
    45181 ctgaaccagc ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa
    45241 cgtctgtaaa cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca gactcatcgg
    45301 ccagcgtgcc gcacgacctt ttatcaataa cgcaatggct ataatcggta acaacggcag
    45361 gacaagtaac gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat
    45421 atcctgctga tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc
    45481 caaaccatac tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac
    45541 agatagatca tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa
    45601 ttcatggcaa ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg
    45661 ttgtcgccac tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg
    45721 ttaccaaggg cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga
    45781 tacggcgcgc catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag
    45841 cgtcgtgaag ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt
    45901 gagtgaagcg ctggcaagcc agccggaatc gccctccgtg cccattcata accaaatccg
    45961 cggtgatgat ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat
    46021 gtacgcaacg ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg
    46081 ctctgcgcgg cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt
    46141 tgcctggaag ctgatgaaga ttggcttcaa tgctgccgca ctgcgcgata tgcatgcgct
    46201 actcgcaaca gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg
    46261 tgtacgacgc gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct
    46321 ggcgattacc ggctttactc cgaatgccct gcaacagcgt gcttctcatt gcctgtatac
    46381 cattgccgaa gaacaagcga caaacagtgc ttcaatctct gcttgtcacg ctcagggaat
    46441 gttaacggat ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg
    46501 tattcgtcat agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat
    46561 gcccgcccgg tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat
    46621 gcatcaattg tgatatgtgt gaaatcaaat aaaaacacat ttattttcat agtgatacag
    46681 caatgtgtta ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc ataagttcag
    46741 caacacaaag aacaccactt tctttaacgt cctccgcgcc ctaaaggacg gggctttacg
    46801 gcgcaaaagg cctgtgacat acaacattaa cttttcagtc aaccgtagca cgttcttgta
    46861 tacgccgtgt ctgcggtttt atataaccct ggctggaaac ctcttataca aagtcgattc
    46921 gccaacatca tagatgatcg ccaccttctg gcgagaaact cctgatgcaa ttagtcgccc
    46981 ggcctgcgcc cattattctg gtgaaagttt gggacggcga ccgccaattc gtccctgtgc
    47041 gcgagcagct tccagtcctg cttttgttcg ttcaacaatc agttcacgtt ccatttcagc
    47101 cagggcaccc atcacatgga agaaaaagcg tcccattggt gtgctggtat caattgaatc
    47161 agtcagacta cgaaagttga tgcctcgttc gcgcaactcc tccaccagca cgacaagatg
    47221 ccgcatactg cgccccagcc gatccagctt ccagacaacc agtgtgtcgc ctgccgataa
    47281 tgtcttgagc agttttttca gccccggtct ttcggacttt gtaccactta tcttgtcttc
    47341 aaaaatcagc tcgcatcctg cacagttcaa cgcattacgt tgtagatcgg tattctggtc
    47401 atttg
//
LOCUS       DADLCQ010000015        43924 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000015 DADLCQ010000000
VERSION     DADLCQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.015"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(15..602)
                     /gene="mog"
                     /locus_tag="JQG95_000772"
     CDS             complement(15..602)
                     /gene="mog"
                     /locus_tag="JQG95_000772"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY5887427.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(717..1670)
                     /gene="tal"
                     /locus_tag="JQG95_000773"
     CDS             complement(717..1670)
                     /gene="tal"
                     /locus_tag="JQG95_000773"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY5887428.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1949..3378
                     /locus_tag="JQG95_000774"
                     /pseudo
     CDS             1949..3378
                     /locus_tag="JQG95_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            3448..4224
                     /gene="yaaA"
                     /locus_tag="JQG95_000775"
     CDS             3448..4224
                     /gene="yaaA"
                     /locus_tag="JQG95_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY5887429.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(4377..4673)
                     /locus_tag="JQG95_000776"
     CDS             complement(4377..4673)
                     /locus_tag="JQG95_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY5887430.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPSPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="JQG95_000777"
     CDS             complement(4887..6173)
                     /gene="thrC"
                     /locus_tag="JQG95_000777"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY5887431.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="JQG95_000778"
     CDS             complement(6174..7106)
                     /gene="thrB"
                     /locus_tag="JQG95_000778"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY5887432.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVVSGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="JQG95_000779"
     CDS             complement(7108..9570)
                     /gene="thrA"
                     /locus_tag="JQG95_000779"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY5887433.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="JQG95_000780"
     CDS             complement(9651..9716)
                     /gene="thrL"
                     /locus_tag="JQG95_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY5887434.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(9930..10616)
                     /locus_tag="JQG95_000781"
     CDS             complement(9930..10616)
                     /locus_tag="JQG95_000781"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY5887435.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTALAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11016..11156)
                     /locus_tag="JQG95_000782"
     CDS             complement(11016..11156)
                     /locus_tag="JQG95_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887436.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            11252..11968
                     /gene="arcA"
                     /locus_tag="JQG95_000783"
     CDS             11252..11968
                     /gene="arcA"
                     /locus_tag="JQG95_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY5887437.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12027..13379)
                     /gene="creD"
                     /locus_tag="JQG95_000784"
     CDS             complement(12027..13379)
                     /gene="creD"
                     /locus_tag="JQG95_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY5887438.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFLLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFILALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13437..14861)
                     /gene="creC"
                     /locus_tag="JQG95_000785"
     CDS             complement(13437..14861)
                     /gene="creC"
                     /locus_tag="JQG95_000785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY5887439.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14861..15550)
                     /gene="creB"
                     /locus_tag="JQG95_000786"
     CDS             complement(14861..15550)
                     /gene="creB"
                     /locus_tag="JQG95_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY5887440.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15563..16036)
                     /gene="creA"
                     /locus_tag="JQG95_000787"
     CDS             complement(15563..16036)
                     /gene="creA"
                     /locus_tag="JQG95_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY5887441.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16247..17116
                     /gene="robA"
                     /locus_tag="JQG95_000788"
     CDS             16247..17116
                     /gene="robA"
                     /locus_tag="JQG95_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY5887442.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="JQG95_000789"
     CDS             complement(17113..17760)
                     /gene="gpmB"
                     /locus_tag="JQG95_000789"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY5887443.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWVERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17812..18332
                     /gene="yjjX"
                     /locus_tag="JQG95_000790"
                     /pseudo
     CDS             17812..18332
                     /gene="yjjX"
                     /locus_tag="JQG95_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
     gene            complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="JQG95_000791"
     CDS             complement(18417..18743)
                     /gene="trpR"
                     /locus_tag="JQG95_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY5887444.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="JQG95_000792"
     CDS             complement(18833..20770)
                     /gene="sltY"
                     /locus_tag="JQG95_000792"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY5887445.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            20981..22648
                     /gene="ettA"
                     /locus_tag="JQG95_000793"
     CDS             20981..22648
                     /gene="ettA"
                     /locus_tag="JQG95_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY5887446.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="JQG95_000794"
     CDS             complement(22956..24188)
                     /gene="nadR"
                     /locus_tag="JQG95_000794"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY5887447.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(24209..25591)
                     /gene="radA"
                     /locus_tag="JQG95_000795"
     CDS             complement(24209..25591)
                     /gene="radA"
                     /locus_tag="JQG95_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY5887448.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSGDLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(25640..26608)
                     /gene="serB"
                     /locus_tag="JQG95_000796"
     CDS             complement(25640..26608)
                     /gene="serB"
                     /locus_tag="JQG95_000796"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY5887449.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            26714..27358
                     /locus_tag="JQG95_000797"
     CDS             26714..27358
                     /locus_tag="JQG95_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY5887450.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27386..28413
                     /gene="lplA"
                     /locus_tag="JQG95_000798"
                     /pseudo
     CDS             27386..28413
                     /gene="lplA"
                     /locus_tag="JQG95_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            28445..28708
                     /locus_tag="JQG95_000799"
     CDS             28445..28708
                     /locus_tag="JQG95_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5887451.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(28869..29588)
                     /gene="deoD"
                     /locus_tag="JQG95_000800"
     CDS             complement(28869..29588)
                     /gene="deoD"
                     /locus_tag="JQG95_000800"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY5887452.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(29645..30868)
                     /gene="deoB"
                     /locus_tag="JQG95_000801"
     CDS             complement(29645..30868)
                     /gene="deoB"
                     /locus_tag="JQG95_000801"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY5887453.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(30920..32242)
                     /gene="deoA"
                     /locus_tag="JQG95_000802"
     CDS             complement(30920..32242)
                     /gene="deoA"
                     /locus_tag="JQG95_000802"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY5887454.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(32369..33148)
                     /gene="deoC"
                     /locus_tag="JQG95_000803"
     CDS             complement(32369..33148)
                     /gene="deoC"
                     /locus_tag="JQG95_000803"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY5887455.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            33406..34947
                     /locus_tag="JQG95_000804"
     CDS             33406..34947
                     /locus_tag="JQG95_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY5887456.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVGILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            34919..35782
                     /gene="yjjW"
                     /locus_tag="JQG95_000805"
     CDS             34919..35782
                     /gene="yjjW"
                     /locus_tag="JQG95_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY5887457.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(35996..36778)
                     /locus_tag="JQG95_000806"
     CDS             complement(35996..36778)
                     /locus_tag="JQG95_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5887458.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKHHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(36775..37848)
                     /locus_tag="JQG95_000807"
     CDS             complement(36775..37848)
                     /locus_tag="JQG95_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY5887459.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYEPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(37970..38131)
                     /locus_tag="JQG95_000808"
     CDS             complement(37970..38131)
                     /locus_tag="JQG95_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY5887460.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(38248..38853)
                     /gene="osmY"
                     /locus_tag="JQG95_000809"
     CDS             complement(38248..38853)
                     /gene="osmY"
                     /locus_tag="JQG95_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY5887461.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(39246..40835)
                     /gene="prfC"
                     /locus_tag="JQG95_000810"
     CDS             complement(39246..40835)
                     /gene="prfC"
                     /locus_tag="JQG95_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY5887462.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(40926..41603)
                     /gene="yjjG"
                     /locus_tag="JQG95_000811"
     CDS             complement(40926..41603)
                     /gene="yjjG"
                     /locus_tag="JQG95_000811"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY5887463.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(41618..42064)
                     /gene="rimI"
                     /locus_tag="JQG95_000812"
     CDS             complement(41618..42064)
                     /gene="rimI"
                     /locus_tag="JQG95_000812"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY5887464.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHSFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(42033..42446)
                     /gene="holD"
                     /locus_tag="JQG95_000813"
     CDS             complement(42033..42446)
                     /gene="holD"
                     /locus_tag="JQG95_000813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY5887465.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            42549..43580
                     /gene="rsmC"
                     /locus_tag="JQG95_000814"
     CDS             42549..43580
                     /gene="rsmC"
                     /locus_tag="JQG95_000814"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY5887466.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        10335 a        12001 c        11129 g        10459 t
ORIGIN      
        1 tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct gaatgcagca
       61 accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg
      121 ctggcaaaaa taccatgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc
      181 tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt gcgaatcacg
      241 cccacctgac gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt
      301 tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca
      361 cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc
      421 agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt
      481 tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgctggaat gcctttatcc
      541 tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc
      601 atcaagattt ttccgtttaa acaatctcgt taaacagaat gatacactgc gaagggagtg
      661 acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gaatgattac
      721 agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt acggatacct
      781 tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca
      841 gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg ttcgatagcc
      901 ccttcgctct ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcacagcct
      961 gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa
     1021 ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc
     1081 ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac
     1141 ggcgagatca ggaacacgcc cgcctccgca caagcacgag cctgagcgaa ggagaacagc
     1201 agggtcaggt tacagttgat accttctttt tccagctgtt ctgcagcacg gataccctgc
     1261 caggtagaag ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt
     1321 ttgatcaggc gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca
     1381 acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat atttactgcc
     1441 agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag
     1501 gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga
     1561 gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg
     1621 tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt
     1681 ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa
     1741 cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac
     1801 actgcgttga tatcgcccac catttttata caaaacctca tgtatgctac gcagaagtta
     1861 tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc
     1921 atcgcgaccg gcgataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt
     1981 cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg
     2041 caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat
     2101 tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcggcgcg
     2161 cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gaccaggtgc
     2221 cgtcttttgg atgtgggttg ctgcgtttat cggcatggcg acctcgtttg ccgaatgttc
     2281 ccttgcacaa ctttataaag aacgtgacgt caatgggcag tttcgtggcg gaccggcatg
     2341 gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct
     2401 catcgcctac ggcataattt tcagcggagt tcaggcgaac gccgttgccc gagccctgag
     2461 tttttctttt gattttcctc cgctggtgac gggcattatt ctcgctgtct ttgctctgct
     2521 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     2581 ggcgataatc tgggtactga ccagcctggt gatttgcgta ataaatatcg ggcaacttcc
     2641 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     2701 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     2761 tgaggtggga atgggatcga cgccaaacgc ggcagcggca gctgcatcct ggcctccgca
     2821 tccggcagca caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     2881 tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga
     2941 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctagg gtgctgaatt
     3001 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     3061 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     3121 ctgcaccttc gcaacgatca tcggcggcac cttgctaagc cttccgctga tgtggcaact
     3181 ggcagatata taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
     3241 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     3301 gtgtttgatc cgttgcgtta tccggatatc ggtcgccaac tttctccgga cgcgtgggat
     3361 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc
     3421 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
     3481 gattaccaaa gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc
     3541 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc
     3601 atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc
     3661 acgccggcga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
     3721 caggccgaga ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     3781 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     3841 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     3901 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     3961 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     4021 gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc taagaaagca
     4081 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
     4141 ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg cgaactggtg
     4201 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
     4261 atccggccta caaaagcatg cagattcaat atattgcaga attaacgtag gcctgataag
     4321 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
     4381 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
     4441 cgcggcggtg acggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
     4501 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
     4561 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
     4621 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
     4681 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
     4741 tgttattctc cgcaatccta atctctaagt taccgcattt attgataatt tctccttttt
     4801 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
     4861 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
     4921 gaaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
     4981 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
     5041 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
     5101 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
     5161 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
     5221 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
     5281 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
     5341 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgaa tgacttcgcc
     5401 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
     5461 cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
     5521 cggctgatgt taatcgagtt agccgagttt aaccccagcg ccactttcag ttcttcatca
     5581 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
     5641 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
     5701 ggatagagga taaccacttt cacattcggc aaaccgtaga aagcatgagc cactgcagct
     5761 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcatccgc aatatgggtc
     5821 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
     5881 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
     5941 gcgcgcacgc gctcttccag gatttcctgt gggatttcat caccaataaa cgccgagagg
     6001 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
     6061 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
     6121 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
     6181 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
     6241 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
     6301 agagcgaaca aggtcgggcc ggagccggag ataccgctca ctaccgcgcc gatttccgcg
     6361 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
     6421 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
     6481 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
     6541 gcccgggctt ctgccgtcga gactttaatt cccggatacg ccagcaccca cagccactca
     6601 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
     6661 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagatacg tccttccagc
     6721 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccgcagtg ttcattcatc
     6781 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
     6841 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
     6901 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
     6961 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
     7021 ccatcaacgg gtgccaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
     7081 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
     7141 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagcac
     7201 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
     7261 tttgaacagc ggatcattcc catccacttc ggcaatcttc acgcggcaga caccatcttc
     7321 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
     7381 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
     7441 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
     7501 cgtttcacga gcgagaatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
     7561 cgggtccggt tcggtataac ccatttcccg cgccagcgtg gtcgcctcgg agaaactcat
     7621 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
     7681 caccaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
     7741 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat aacgcaactg
     7801 atgatagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtaacaa cgtggaaacc
     7861 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
     7921 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
     7981 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacactagt
     8041 gagcagagcc ttcgagttgg caacgccgca gacacgtaag tcgatatgtt tattcttcag
     8101 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
     8161 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
     8221 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
     8281 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
     8341 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
     8401 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
     8461 agctcgcaca cagtcgcttt gtggaacgca gaaactgata ctgtattcgg aagatgattg
     8521 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
     8581 gccaaccatc cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
     8641 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatga gcgtacctgg
     8701 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
     8761 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
     8821 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
     8881 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
     8941 ggagtagtcg gaaccgttac gtccaagcac caccagttcg cctttttcat taccggcggt
     9001 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
     9061 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
     9121 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
     9181 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
     9241 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
     9301 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
     9361 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
     9421 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
     9481 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
     9541 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
     9601 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     9661 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
     9721 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     9781 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     9841 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
     9901 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     9961 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
    10021 cagtcgacca gttttatgtc atctgccact gccagagccg tcagcaatgc catgactcgt
    10081 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
    10141 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    10201 taatccgcca ccatcggcac accagtaagc acgtcagcca acgctaactc ttcgttagtc
    10261 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    10321 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactacgc
    10381 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagtgt cgggaaaact
    10441 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
    10501 tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
    10561 gctgccccaa tattttctgc tctggcgggt gcgaccagga taatcgttat acgcatattg
    10621 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    10681 cgaatttaca aattcttaac gtaagccgca gaaaaagccc tttacttagc ttaaaaaagg
    10741 ctaaactatt ttctgactgc actaacggtt taattgttaa aaaatgctac atatccttct
    10801 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    10861 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    10921 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    10981 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    11041 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    11101 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    11161 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    11221 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    11281 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    11341 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    11401 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    11461 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    11521 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    11581 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    11641 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    11701 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
    11761 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    11821 cggccgtgag ctgaaaccgc acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    11881 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    11941 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    12001 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    12061 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtctat attacgagtc
    12121 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    12181 tcggcagagt tgagcagtcc ccacatcact ccatccagca acaacagcgc gaggataaac
    12241 aacatgctat tgcgccagcc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    12301 ccaatcagac tggcgattat ccatgccacg gtaaaaccga tatgttcaga aagcgccagc
    12361 aagagcaaat aaaacatcac caatgaaagc cccaccagca aatattgcat tgggtgtaaa
    12421 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagtgc aatcagcaga
    12481 atggcgtact tagtcgcccg gtccgttaat tggtattgat cggctggcgt cgtcactgcg
    12541 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    12601 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc gctagcttcc
    12661 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    12721 atttcgctat tacgcccggc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    12781 gccatattca gctgcaggtt ctgcttacgc cagtcccctt cgggtaaagg gatatgcacg
    12841 ccctgcccgc cttgctctaa cccggtaccg ggttcaatgg tcagtgccgt tccgttaact
    12901 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaagggc
    12961 ttgcctaaga tgatatttgg cgcgttgagc tcgctaagac gcgaaacatc gaaatcggct
    13021 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    13081 acgttctgat tgccatcaac catcaatgac tcaggtaaca aaaaatggat aaaacttcgt
    13141 ttccgctcca cggttttatc ctcttcctgc accgtataaa gctcggtcac aggaatagcg
    13201 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    13261 tcgctacggt aatcggcacg ttcgacaatc acttgccgaa tcagcattat cggaatcaac
    13321 aacagcaata ctgcaccaaa caaggtggtc attttccaga acagggggga tttcaacata
    13381 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    13441 gtgaagtgac ggtgaagtcg tagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    13501 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    13561 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    13621 tagtcaggaa taccactacc ggtatccagc accttaagcg tgacgtgttc ctgatccact
    13681 tcggcgctta gcgttatgca accgctctcg ggagtaaaat cgatggcgtt atccagtaaa
    13741 ttccccagcg cctgatccag taacgccggt tcagcagcaa cattaacctc ggtgggcata
    13801 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    13861 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    13921 tggcgtagta acgtttccac cagcgcctgc attcgcgcat tttgtgtcag aatgttgtca
    13981 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    14041 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    14101 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcgagttt acgcagctcg
    14161 ctactaccga gatcgggaag aggaacgggc ttattgtcag tgacggaatc agcatagcga
    14221 gtgagccggg caatggagcg gttgatccac caaaccatgc ctgcgccaat caccagtgca
    14281 atccccaaca aaatggcgct ggcccataaa attcgccgct cgctgcgttt aatgaccgga
    14341 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgcccata
    14401 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    14461 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    14521 ccaacggctt tatttgccga atcgaacaat actttgccat gcgcatcggt catatagaca
    14581 tgatattcat tgcgcacttt gttaatgcca ccaatattgg cgcgaaacgg gcgatgttgt
    14641 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    14701 cgcgccagct ccgcaagtaa cgttgccgtg tcgatcaacg tcccctccgt tgctcttcgc
    14761 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    14821 agtaaaaaat agcccagcag caaccgcatg ccgatacgca ttacaagccc ctcaggctat
    14881 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    14941 cacgcagcgt tttaatgtgg atatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    15001 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    15061 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg
    15121 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gtcgagaact
    15181 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    15241 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    15301 gggccgtcag gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt
    15361 caaagccact gatatcaggc aaaccaacgt cgagaatcat gacgtcggga acctgctggc
    15421 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    15481 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    15541 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    15601 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    15661 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    15721 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    15781 cgacttgctg acaagaaatg gccgcatcgg aggtatcttc tgccaggccc aatccccctt
    15841 taataccacc ggttttcgcc cggctcacat aacaggtgac atttttcaca tcgggatcgt
    15901 caaaggcttc cacaacaatt ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg
    15961 aaccaatctc ttctgcatga gccaatggcc ccagcattat caggcttaaa gacaggatca
    16021 aatgcttgta tttcatattg ttaccatttt tatctattac acgagcaatt agttcgtcac
    16081 ggtaaataac atattgatta agaacaaaat ctcaattctt ttatttccgt caagccctaa
    16141 aacatactct actaagaaaa aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca
    16201 attacctgat gtcaggtgct cgttgttgaa aggatgagga tattttatgg atcaggccgg
    16261 cattattcgc gaccttttaa tctggctgga aggtcatctg gatcagcccc tgtcgctcga
    16321 caatgtagcg gcgaaagcag gttattccaa gtggcactta cagagaatgt ttaaagatgt
    16381 cactggccat gctattggcg cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc
    16441 actacgcctg actgcgcgtc cgattctgga catcgcgctg caataccgct tcgactctca
    16501 acagacattt acccgcgcat tcaagaagca gtttgcccag actcctgcac tttaccgccg
    16561 ttctcctgaa tggagcgcct ttggtattcg cccgccgcta cgtctgggtg aattcacaat
    16621 gccagagcac aaatttgtca ccctggaaga tacgccgctg attggtgtta cccagagcta
    16681 ctcctgttcg ctggagcaaa tctctgattt ccgccatgaa atgcgttatc agttctggca
    16741 cgattttctc ggcaacgcgc cgaccattcc gccggtgctc tacggcctga atgaaacgcg
    16801 tccgagtcag gataaagacg acgaacaaga ggtattctat accaccgcgt tagcccagga
    16861 tcaggcagat ggctatgtac tgacggggca tccggtgatg ctgcagggcg gcgaatatgt
    16921 gatgtttacc tatgaaggtc tgggaaccgg cgtgcaggag tttatcctga cggtatacgg
    16981 aacgtgcatg ccaatgctca atctgacgcg ccgtaaaggt caggatattg agcgatacta
    17041 cccggcagaa gacgccaaag cgggagatcg cccaattaat ctgcgctgtg aactgctgat
    17101 tcctatccgc cgttaacgct gcagctcatc taatgcaggg gcgtccagat gcgagatgtc
    17161 ccctgcggtt tcaacgaccc agccagacgc cagccacagg ctttcctgat aatccacgcg
    17221 cgaaatagaa cagttacgca gacgtaagcg gcgctctacc catgctggta atccgagaat
    17281 cgtactcacc agacatccca gtgcaatacc atggctcacc agcagcggtc ggcttccctg
    17341 cggtaagtcc cggcaagatt ccagtgccgc attgacgcga tcgctgagct cctgcattga
    17401 ctcgccttca ggaatacgcc cgtcaacggt gccattgacc agctgccgac gccagttctc
    17461 ttcttcttcg gtcagagaat cgatatgtct tttttccagc acacccatgt ttaattcacg
    17521 caggcgagaa tcaaagatga tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg
    17581 ccgggtgcgt cctaaatcgc tactgataat atgcgtaatg ccaagctctt tggcacgggt
    17641 tgccacctgc atcgcctgtt gctcaccttt ggcggtcagc gggctgtcag actggccctg
    17701 aatacgtcgc tcggcgttcc actgcgtttc accgtggcgg actaggtata cctgtaacat
    17761 gctttttttc cgtatactgc tgtaacgtta attttctgag taatgctgat tatgcaccaa
    17821 gttgtctgtg cgaccaccaa tcccgctaaa attcaggcca ttctgcaggc atttcacgag
    17881 atcttcggcg aaggatcctg ccatattgca tccgttgccg tcgagagcgg tgtaccggaa
    17941 caaccctttg gcagtgagga aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc
    18001 cgtttacttc cagaggctga tttttgggtg gcgattgaag ctggcattga tggcgacagc
    18061 actttcagtt gggtggtgat tgaaaacgcc agccagcgcg gcgaggcgcg ttcggcaact
    18121 ttgccgttac ctgcggtgat tctggaaaaa gttcgtgaag gcgaggcgct tggtcccgtg
    18181 agtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    18241 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    18301 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    18361 acattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    18421 tcgcttttca gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg
    18481 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    18541 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    18601 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    18661 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    18721 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac
    18781 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    18841 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt
    18901 aagcatcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    18961 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    19021 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    19081 catattgcag gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag
    19141 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    19201 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    19261 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    19321 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    19381 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    19441 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat
    19501 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc
    19561 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg
    19621 ctgcaaccat cgggtagaaa ccacgctgtt gtatgagttg atgcaaaatc tctttcgctt
    19681 cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct
    19741 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    19801 caagcgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt
    19861 catcgcgcca tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga
    19921 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cgcctgggca agcgacggga
    19981 tcatcagccg tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca
    20041 tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt
    20101 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    20161 ccagcactgt taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat
    20221 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    20281 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    20341 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    20401 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    20461 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    20521 tcaccgccgg ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa
    20581 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc
    20641 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    20701 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    20761 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    20821 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    20881 actgcgaagg tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg
    20941 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    21001 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    21061 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac
    21121 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    21181 agacatcaag attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    21241 tgagtccatt gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt
    21301 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    21361 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    21421 ggcggatgcg ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga
    21481 gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    21541 cgaaccgacc aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga
    21601 cttcgaaggc accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg
    21661 ctggatcctc gaactggacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg
    21721 gctggagcag aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    21781 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    21841 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga
    21901 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    21961 caacctgcgt aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc
    22021 gaaaggggcg atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    22081 tatgatctcc ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa
    22141 actggcatcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    22201 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    22261 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg
    22321 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    22381 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    22441 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    22501 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    22561 tactgagtac gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg
    22621 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct
    22681 tatccggcct acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg
    22741 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc
    22801 cggtctacaa cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc
    22861 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc
    22921 attttatacc tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc
    22981 cgcaccagct ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg
    23041 aattcgatat tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc
    23101 accgaactgc cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg
    23161 atcaccagat cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct
    23221 tcgtactttt tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta
    23281 ttggcgtatt tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag
    23341 tcggaatact gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca
    23401 tattcccacg cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta
    23461 ccgctcgact cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta
    23521 ggaatatatt cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata
    23581 aaggtacgtt tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc
    23641 gcatcggctt cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac
    23701 tttttgatgc cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg
    23761 ttgaaagcat gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga
    23821 cgatccggca ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg
    23881 gtatcgtcaa aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata
    23941 aggtagatat gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc
    24001 ttctgccggg gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg
    24061 gggcttttaa ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca
    24121 tcagctacct gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat
    24181 gacgacatat ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg
    24241 agagtttttt aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg
    24301 ccggaacaat cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac
    24361 tgggcaccgg gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg
    24421 gcagcggtct gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcgccgctgg
    24481 tttcggttac cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca
    24541 gaccaccgtg acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca
    24601 ctgccacgcg gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca
    24661 gcaacggacg cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc
    24721 cgcgacttaa gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg
    24781 cgaagacgcc cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa
    24841 aacgggagtc ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt
    24901 tcgggccagc cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc
    24961 gcgttttggc gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag
    25021 gcgatgattg tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg
    25081 gttgctcttc ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga
    25141 gattgtcagt cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt
    25201 cgccggtgac atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca
    25261 gcgtggattt ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc
    25321 cgcctcctag tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa
    25381 ggctgatatc ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga
    25441 gacgctcgtt acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc
    25501 aggcatgaca ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac
    25561 aaacaaaggc gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg
    25621 caggatgacg ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc
    25681 ccatcaggtc agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat
    25741 ggtaggcaat ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa
    25801 tcgccacggt ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg
    25861 ctttgtactg cgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga
    25921 tctccagttc attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa
    25981 agaaagtaaa gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca
    26041 ccagctgcgt taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg
    26101 cgtcagcgcc tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc
    26161 cgcgcatcgc ccgttcggtt acctccgcca ccatctcgcc cgttccggcc agcttggcaa
    26221 tttcatcaat acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg
    26281 gcgtgcgcag gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca
    26341 ggcgtgtagc ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc
    26401 accaggcggc aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct
    26461 gtttatccag cccacgacca tacagcagcc agccgctacg acctgcgtgg taatccagtg
    26521 gcatcacttc atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag
    26581 gcaggtcgca ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga
    26641 aaataacgca tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt
    26701 caaagggtgg aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt
    26761 cctgttctgt cttgccttgt tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa
    26821 ccaccagcga cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt
    26881 gacgctgaac gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc
    26941 gatcctcgat cagttaacag atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca
    27001 aggcgatctt atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga
    27061 cggtaaaaaa gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa
    27121 cggaccgctc ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca
    27181 acaggtggat aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg
    27241 cgtagtgctg acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt
    27301 cctgttaacc gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc
    27361 cattactaca agaaaggaaa tcgttatgtc cacattacga ctgctcatct ctgactctta
    27421 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
    27481 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
    27541 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
    27601 tggcggtggc ggtggcggtg gcggcgcggt gttccacgat ctcggcaata cctgctttac
    27661 ctttatggct ggcaagccgg agtacgataa aactctctcc acgtcgattg tgctcaatgc
    27721 gctgaacgcg ctcggcgtca gcgccgaagc gtccggacgt aacgatctgg tggtgaaaac
    27781 cgtcgaaggc gaccgcaaag tctcaggctc ggcctatcgc gaaaccaaag atcgcggctt
    27841 ccaccacggc accttgctac tcaatgccga cctcagccgc ctggcaaact atctcaatcc
    27901 ggataaaaag aaactggcgg cgaaaggcat tacttcagtg cgttcccgcg tgaccaacct
    27961 caccgagctg ctgccggggc tcaccatgag caggtttgcg aggccataac cgaggccttt
    28021 ttcgcccatt atggcgagca cgtggaagcg gaaatcatct ccccggacaa aacgccagac
    28081 ttgccaaact tcgccgaaac ctttgcccgt cagagtagct gagagtggaa cttcggtcag
    28141 gctccggcat tctcgcatct gctggatgaa cgctttagct ggggcggcgt ggaactgcat
    28201 ttcgacgttg aaaaaggcca tatcacccgc gcccaggtat ttaccgacag cctcaacctc
    28261 gcgccgctgg aagccctcgc cgggcgactg caaggctgcc tgtaccgcgc ggatatgctg
    28321 caacaagagt gcgaagcgct gttggttgac ttcccggacc aggaaaaaga gctacgggag
    28381 ttgtcgacgt ggatagcggg ggcggtaagg taactcgctg attaaaatat ccaaggaagg
    28441 aaatatgatc gatctggaaa accaggaacg ggagattatc aatttaatgt tcagccagcg
    28501 tatttcctgg ctagccgccg tgcgtatcag gcataaatta tcgcttgctg aagtaagtaa
    28561 aatgttaggt atctcgatta attctctaaa acagatcgaa aagacggaac ggcttagctc
    28621 aaacatcaaa agcaaaatgg cagaaattta tggttgtcca ccagagttgc tgatatgccc
    28681 ttcctggatg acggctgaac ataaatgaat agattcgtga atagatactt gaatgttttt
    28741 tattttctat ccatatgaaa aataatagta aatctataaa tcagttgcta ttgatgaata
    28801 gatactaaaa agccggagca gtcccccact ccggcttaac acgacaatcg cctttcagcg
    28861 aaacacaatt actctttatc gcccagcaga acggattcca gtgcgatttt gatcatgtcg
    28921 ttgaaggtgg tctgacgctc agcggcagtg gtctgctcgt gagtgcggat gtggtcagac
    28981 acggtgcaga tggtcagggc tttcgcgcca aactctgcag ccacgccgta gataccagcc
    29041 gcttccattt ccacgccgag gatgccgtat ttttccatca cgtcgaacat ttcgccgtcc
    29101 ggagagtaga acaggtcagc ggagaacagg ttacccacgc gagcatcaac gcccagtgct
    29161 ttagctgcat ctactgcgtt acgcaccatg tcaaagtcag cgatagcggc aaagtcatgg
    29221 tctttaaaac ggatgcggtt aactttggaa tcggtgcagg cccccatacc gataacgacg
    29281 tcgcgcagtt ttacgtgcgg cagaactgcg ccacaggaac caacgcggat aattttcttc
    29341 acgccgaaat cggtgatcag ttctttggtg tagatggagc aggacgggat acccataccg
    29401 tgacccatta cggaaatttt gcggcctttg taagtaccag tgaagcccag cataccgcga
    29461 acgttgttca cttcacgggc atcttcaagg aaagtttcag caatatactt cgcacgcagc
    29521 gggtcgcctg gcatcaaaac tacgtcagcg aaatcgccca tttctgcatt aatgtgtggg
    29581 gtagccattg ttttatcctt tttgtgacat aacaaaggcg gagtcaacgc tccgcccaaa
    29641 tccatcagaa catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag
    29701 tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc
    29761 catataccag taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcgc
    29821 aaccgtggtc agcggtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg
    29881 gcagacggcg gtcgaacagt tccagacccg cagcataacc ggcgacgtcg cggcggtggc
    29941 cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt
    30001 tcatctcttt gatggtggcg tcaaacagcg cgtccaagcc agtcgctttc acttttttgg
    30061 tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tggccgtgtt
    30121 tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aagtcgtgac
    30181 ggttaccggt acgctggaag ttaccagctt tgtcaccgat aaacggacga gcgataacac
    30241 gaccgatatt gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga
    30301 gtttatccag accgaaagtt tcttcatggc aggcaatctg gaacacggag tcagcggagg
    30361 tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagttgatcc agaatgaccg
    30421 taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttcg accagtttat
    30481 ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaacagaa
    30541 ccgggacacc ggcgatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt
    30601 gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac
    30661 cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg
    30721 gccctttacg accattatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg
    30781 tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt
    30841 ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa
    30901 tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg
    30961 gtgcgttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt
    31021 tttcgtcttt cgcgaggata accgccagcg gacgctgacc gtctacctgg tcgcccagac
    31081 gcgccatatc agtaaagccg acgctgtaat cgatggtgtc agatgcctga cgacgaccgc
    31141 cgcccattgc aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt
    31201 cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct
    31261 caacgaagtc ggtcgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt
    31321 taccgttgtc cagcaccgcc tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt
    31381 tgccggagat cagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac
    31441 gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg
    31501 aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag
    31561 ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc
    31621 cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgccagt ttcttcgcca
    31681 ggatcgaggc agtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt
    31741 agaaacgttt atcagccgga gccagtgaac tggtctgacc gataatcgcc acgccgacgt
    31801 ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt
    31861 ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accggagatc atcggaatat
    31921 agccgccgca ggctgcgacc atcggcccca acatcagcga agtgacatcg ccgacgccgc
    31981 cggtcgagtg tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg
    32041 ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga
    32101 agaaaatggt catcgcgagg gcggcaatct gcccttcgga aatagtgttg tcgcgaatac
    32161 cgttgataaa gaaacgaatt tcttcatcgc tcagcgcatg accatcacgt tttttacgaa
    32221 taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg
    32281 ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc
    32341 gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcgccgt
    32401 ggcccagcgc tttcagcagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg
    32461 catcagccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt
    32521 ccgcagtacg cacgccgccc gccggtttga aaccaacggt tttttctacg cccatatcac
    32581 ggatcacttc catcatgatg cgagcgcttt ccggcgtcgc gttcacagcc actttaccgg
    32641 tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca
    32701 gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg
    32761 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtca cccgccatca
    32821 gcgcgcggta cgggaacacc acgtcaactt catcagcacc gtaggcgatt gccgcacggg
    32881 tttctgccag cgcgatttcg atgtcgtcgt taccgtgtgg gaagttggtt accgtagcga
    32941 tacggatttc cggggtgccc tgctctttca gagttttgcg agcaatcggg ataaaacgag
    33001 gatagataca gatagcggcg gtattgccga ccggagtttt ggcctgatga cacagagcaa
    33061 ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    33121 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    33181 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tacacatcac
    33241 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    33301 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    33361 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    33421 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag
    33481 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttaccc acagcttcct
    33541 gccgaagccc gccgcgcgct ggatgaaggt gtaatctgcg atatgtttga aggtcatgcg
    33601 ccgtacaaac cgcgctatgt cttacccgat tacgctcgtt ttctggcgaa cggttccgaa
    33661 tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt
    33721 taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    33781 ttgcaaccgt atgttggaat tctaacacaa aacgagatcg atgttcgaat aaaacgtttc
    33841 tggcgttacc tcgacagaac cctgccagat gcctttatgc acgccaatat cggcccgtct
    33901 gattcgccca ttacccgcgc gatcttacgt gcagacgcgc aggtttcacc gaacctgacc
    33961 tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc gaagaacatc
    34021 tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat tttcacaaaa
    34081 gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg tggcagcacg
    34141 ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga tgacttcttt
    34201 acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc gcggtgtgaa
    34261 ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga agggctgatt
    34321 aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc ggttaacttg
    34381 ctgtgtgaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc aaatgaagtg
    34441 ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt gaaatatggc
    34501 tggcaaaaac gcgccatgtt acacgcacag tcggggatca gttccgatat cggcaccacg
    34561 ccgggcgcgc gtttgccgta tggcgatgag ccagatccga tcacccatct gcaaactgtc
    34621 gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct cgacgaaacc
    34681 atcaaacgta acccgcaggc actggtacag ctttgcctcg gtgcctttaa agccggaatg
    34741 cgtgaattta ccgccaatgt cagcggtaac gatctggttc gcgttaccgg ttatatggtg
    34801 cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac cacctggctg
    34861 ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt gataagccat
    34921 gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg acgggccagg
    34981 cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact gtcacaatcc
    35041 gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc cgcatcaggc
    35101 gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtctgcgagc aatgtgatac
    35161 ctgcctgaag atgtgtccgc aacatgccac accaatggcg caatccatga gcgtggacga
    35221 agtgcttagc catgtgcgca aagcggtgct gtttattgaa gggataacgg taagcggcgg
    35281 cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca aaaacgatcc
    35341 gcaactgcgc catctcacct gtctggtgga cagtaacggt atgttgagcg aaaccggctg
    35401 ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcat ggggtagcga
    35461 atgtcatcaa caattaaccg gacgcgataa tcaacagatt aagcgcagca tctatttgct
    35521 ggcagagcgc ggcaagctgg cggaactgcg tttgctgatg atcccaggcc aggtggatta
    35581 tttgcaacac atcgaagaac tggcggcgtt tatcaaggga cttggtgatg ttccggttcg
    35641 cctgaacgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg cgagcgccac
    35701 gccggaagac gttgagccgt tggctgatgc attaaaggtg cgcggggtga gccggttgat
    35761 atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca cagacttgta
    35821 ggcctgataa gacgcgtcaa gcgtcgcatc aggcattgtg cgccaactgc cggatgcggc
    35881 gtgaacgcct tatccggcct acggttcggc acaaacctgt aggcatgata agacgcgtta
    35941 agcgtcgcat caggcattat gcgccgactg ccggatgctg cctacgaagg gcaaactacc
    36001 cacgaacgtt aaacaccgca tacgtgttat taagcaacac ttcggcaatc tcatccgcgg
    36061 gttccgggcg taactcgcaa agcacggcaa acacacgggc agcctgttcc gggcggttag
    36121 gctgcccctg aaaaccgttg agcggcatat ccggcgcatc ggtttccagc aacaacgatg
    36181 ccagcggtaa ttttgcgatg acatcgcggg ttttactggc gcgtggatag gtgatagtac
    36241 cgcctacgcc gattttgtag cccagctgta caaaccgttc agcctgttgc tggctgccag
    36301 aaaatccgtg gacaacacca gcacgcggca aatcgtggtg tttaagatgc atcgccagtt
    36361 tgtcgtgcgt gcgccgtgaa tgcaggatca ccggcagatc gtagcgtttc gccagtttca
    36421 gttgttcgtc gagtaaccac tgctgcctct caaattgcgg atcgtcgccg aagagatcca
    36481 gaccgatctc ccccaccgcc accaccttcg ccggacgcct ttccagcgcc tgctgtagct
    36541 gctcaagaga cacatcgcta tgtttttcca acataccagg atgcaagccc aatgcggcat
    36601 acagcggttg ataattttcc gctaacgcct gcacgcgggc aaaattttcc gcctctgtcg
    36661 ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg ttgcaggctg gcctcttcat
    36721 cgccaataaa aggcgggaaa tcaaaatggc agtgagtgtc gataaaacgg caaatcaagc
    36781 cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga gcgtcgctca cttcggcaac
    36841 cagcgtatcg ttggcgactg gcgcaggcgg aatgacaatc gattccggcg tcaccactgg
    36901 caccagatgg cgggtaagcg gcgctttttc agttaatagc ttgccaaccg tggcgaggaa
    36961 ataacgaccg cataaacgcc caagtttata gtcttcacgc agcgccggaa tccgactgcc
    37021 aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg gatatccgca gcttgcccgg
    37081 tggtttctca ataaattgct gaatgtcacg atagctggtt tcatgatgct gcaccagatt
    37141 gaccagcggc tgcaggctac tgtcacccag ccagcgttcc atgcgtttga accactgcgg
    37201 cgtgtagtac atttgtgacg gcacagtgcg gatgacgacc aacgttttag cgccctgcct
    37261 tgccgcctct ttaaccggaa tcgcatcact gatcccgcca tccaggtagt taatgccttc
    37321 cagcgacact ccgctacgat aaaagccagg tatcgcactg gaggcgcgaa tcacatccag
    37381 ccagttttgt ttggttggta aaaagtaatt cggctcgtag tcatcctgac gacaggcgca
    37441 catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg gtatccattt gcaacggcat
    37501 ctggcttgcg gtggcctcca ccagccagtc gagatcgata agatttcctc cacggacaaa
    37561 gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc atgatgactt tgcgcgcgta
    37621 accgggctga ttgcagataa acgccgagag gttctgcgcc ccggcagatg tgccgagata
    37681 aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc agcacgccag ccgtgaaaat
    37741 tccacgctgt ccgccgcctt cgcacaccag agctattcgt ccaggctgga acggccttag
    37801 cgacaacggc gcaatattac caagcgttac aggtattcgc tgccccactt ctgccttcct
    37861 gtttttgttg tgaaagaaag acaaagtaac gcaatttatt attcgctaaa accgcaaaag
    37921 ccagtccgct ggactggctt tggataaaat tttcttaatg gtctgaaatc tagggtcgtt
    37981 ttcggcccat gaacaaactc actaggaaca gaataatccc gacgacaaag acaattttag
    38041 ctgcgcctgc agcggtaccg gccagaccac caaacccaag tgcggcggcg attaacgcga
    38101 taaccagaaa tatgatgccc caacgaaaca taagcgtctc ctttaccata gttaatgtca
    38161 ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt ttagtgtggt gcacatcgcg
    38221 cctcccgaca aagttcggga ggacgaatta cttagttttc agatcatttt taacgctttt
    38281 cacaccatct accgctttgg cgatactttc agcacggtca ctttgtgcct gagaatctac
    38341 ggtaccggag agctgaacca cgccatcggt ggtttcaact ttcactttac gtgaagggac
    38401 gatatcgtcc gccagcagtt tggctttgat ttcactggtg gtggcggtgt cacccgcgta
    38461 gcccttcacc gagccttctt tagcgtcgcg aacgtgcagt ttgtcgctga cagaggtcac
    38521 gccttcaacg cctttcgcca ctttcactgc ctcttcggcc tgggcctgac tttcaacgaa
    38581 accgctcagg gtcacgactt tttgatcggt tttgacagag atatcggtgc tcttgatgtt
    38641 gtcatgatcc accagcgccg ccttcacttt cgcggtgatg gcgctgtcat ccatgaaatt
    38701 accgacttta ttcatagagc tatcgacttt ttgccctgcg ctttcattgg tagtctgcgc
    38761 gttgttttcc gcgtaggcag agccggtcgc gacggcagag gtcaacatta cagccagcag
    38821 agttttcgaa atcttcagtc ttgtcatagt catcgattta ttcctgtatg tttgctcgta
    38881 atttgagcct agcaacacga ggttgcattg ctgaataggg agagacttca ccctctacag
    38941 aagtcaatgg tcgccatcac aaaagcgatg ggtgatgaat aacgaccatt acagcctctg
    39001 aatcagttat taatatcggt agaatgacaa tcgacggctt tagatactga tatctacgca
    39061 ttgaacggta tttaacgcca tcagaaatgt catcactttg ttaaatatag atcacaattt
    39121 tgaaaccgct cgggatatca gcgaaaacat aagcaaaagt gaatgtttta agaacattcc
    39181 gtaagcggct aataaggaag ggaaattgac agggcgcagc ggctaccgcg ccctggaggc
    39241 aagaattaat gctcgcgggt ctggtggaac tgaacgtccg gataacgttc ctgtgccagg
    39301 cgcaggttga ccatgctggt agcgatgtaa gcgaggttat cgccgccatc aagcgccagt
    39361 tggctttcgt tcttacgctt gaactcttcg aatttcttcg cgtctgcaca ttctacccag
    39421 cgggcagtgg caacgttgac tgactcatac actgcttcaa cgttgtattc gctcttcagg
    39481 cgcgctacca ccacatcaaa ctgcagcaca ccaaccgcac caacgatcag atcgttgttg
    39541 gagattggac ggaacacctg caccgcgccc tcttcggaaa gctgtaccag ccctttgagc
    39601 agctgttttt gcttcagcgg atctttcagg cggatacgac ggaacagttc tggtgcgaag
    39661 ttcggaatac cggtgaactt catcatctca ccctgggtaa aggtgtcgcc gatctgaatg
    39721 gtgccgtggt tgtgcaggcc gaggatatcg cccggatacg cttcttcaac gtgcgaacgg
    39781 tcacccgcca taaaggtcag cgcgtcggag atcaccacat ctttcgcagt gcgcacctgg
    39841 cgcagtttca tgcctttttc atatttaccg gacaccacac gcataaacgc cacgcggtcg
    39901 cggtgtttcg gatccatgtt ggcctgaatt ttaaatacga agccggtaaa tttatcttcg
    39961 ctcgcttcta cggtacgggt atcggtctga cgcggcatcg gcgcaggcgc ccactccacc
    40021 aggccattca gcatatgatc aacgccgaag ttacccagcg cagtaccgaa gaataccgga
    40081 gtgatttcgc ccgcaaggaa cagctctttg tcgaactcgt tagacgcgcc tttaaccagt
    40141 tccagttcgt cacgcagctg ctgtgccaga tcttcaccaa ccgcagcatc gagatccggg
    40201 ttattcagcc ctttaacaat gcggacttcc tgaatggtgt gccctttacc gctctgatag
    40261 agataggttt catctttata aaggtggtaa acgcctttaa acagcttgcc gcagccaatt
    40321 ggccaagtga tcggtgcgca gccaattttc agctcgttct caacttcatc gagcagttcc
    40381 atcgggtcgc ggatatcacg gtcaagtttg ttcataaagg tgaggatcgg cgtgtcgcgc
    40441 agacgggtaa cttccatcag cttacgggta cgatcttcaa caccttttgc ggcgtcgata
    40501 accatcaggc agcagtccac cgccgtcagg gtacgatagg tatcttccga gaagtcttcg
    40561 tgccccgggg tgtcgagcag gttaaccagg caatcgtgat acggaaactg catcacagac
    40621 gtagtaatgg agatcccacg ctgcttttcc atctccatcc agtccgactt agcgtgctgg
    40681 ttggaaccac ggccttttac tgtaccggcg gtctgaatgg cctgtccgaa cagcagcacc
    40741 ttctcggtga tggtagtttt accggcgtcc gggtgagaaa taatggcaaa agtgcggcgc
    40801 ttcgccacct ctttcaaata aggagacaac gtcataattc tttcttcttg agtaagcggc
    40861 atcgcgccgc gcttgttgaa aacgaaaaat tgcggctatt ttacccatca acggggggga
    40921 ggcaatcagt gtttacacag gagctgctcc agttcgtgca acgaagaaac ggtccaggtg
    40981 ggcgcgatgc cttctggttg ctcgcgattg tgcgcattca gccagcaggt cgcaagcccg
    41041 gcgttgatgc caccgagaat atcggactcg gcagtgtcgc caaccatcag cacgcgtgaa
    41101 cggtcaggat tgcccgcctg ttccagcgca taatcgaaaa ttttcttatt cggtttggca
    41161 acgccaactt cttcggaaat caccagcaaa tcgaagtaat cacgcaggcc cgtccgttcc
    41221 agacgcactt gctgcaaggc gctaaaaccg ttggtgatga tgccgatttt agcgttgcta
    41281 cgaatggcgt taagcagaga aaccgcgccc ggcaacggcg tacagatttc cgccatcgca
    41341 ttaataaagg catcgttaag caagcctgct tcaactttca gccgttcggc ccagctctcg
    41401 aaacgcccgt gctgaagctg taatgaagtg atcgcgccgt tttgataatc cacccacagt
    41461 ggcttgttaa cggcctgata gtcctgaaaa tcttcagcgg taaaggtgac gctgtaatca
    41521 agaaacatcc gctgcaggcc ggtgaatgag tcaaaggtaa acagcgtttc atcggcatca
    41581 aagaaaatcc agtcccactt cattattcca ccttgtctta catactgatt ggcaacgcca
    41641 tgatgatggc gtcttcgcga ccgtccgtgg tggggtagta attgcggcga atcgtcgcct
    41701 cgttaaagcc taaactttcg tacagggcaa tggcggcagc atttgaggca cggacttcca
    41761 gccatagtgt cgccacgccg cgtttttcca gttcgtcgat cagatgttcc agcagcgccc
    41821 tacccagccc ctgacgctga tagtcgggat cgaccgcaat attgaacaat gtagcttcat
    41881 ccagcaccac ttgcgtaatc gcaaacgccg ccattttgcc gttttgcgtt aactgaaagt
    41941 tgagataacg ctcgccctgg ttgctggcaa aggttttttc actccacgga aaggagtggg
    42001 cgcgttgttc aatgtggtaa gccgctggta aatcagtcgt ttcgagggaa gaaatcgtgt
    42061 tcatatgtgc aaatttgttg ccataacgcg gcgcgtgccg ttgggtttgc ccgtaattcg
    42121 gtgagcgccg gtgatgccac ctgagcgcct tccaatgata gcggttcgtc agtacccaac
    42181 cgccagctgt tgcagcgact gccttgcggc agcatcgcga ttttttctgg cgtcagttgc
    42241 agcacttgat cggggctgac ggttaatgcg cgcagaacat cgctcactaa aggatcagtc
    42301 agggcgggaa gatcgtttgc caccatcacc agacggacgt gtgccgggct cgcaatggca
    42361 atctccccct gcaacgcgcc agggcgacgc agcgaccact gggtaatgcc cagttgctgt
    42421 aactgccagt ctcgtcggga tgtcatagcg aatcgctcct gttgtcaggg gcgcaaatat
    42481 agcaaattcg tcgataccgc gccaacatat ggctataatc gccgccagta tcaattgagg
    42541 agcattccat gtctgcattt accccggcaa gtgaagtctt gctgcgtcac agtgatgatt
    42601 tcgaacaaag ccgtattctg tttgccggag acttacagga tgacctgccc gcgcgtttag
    42661 ataccgcggc cagccgtgct catacccagc aattccacca ttggcaggtg ttaagctgcc
    42721 agatggggga taacgcccgt tttagtctgg tcgccacggc gaatgatgtc gcagattgcg
    42781 atacgctgat ttactactgg ccgaagaaca aaccggaagc ccagttccag ttgatgaatt
    42841 tactttctct gctgccagtg gggacagata tttttgtcgt tggcgagaac cgcagcggcg
    42901 tgcgcagcgc tgagcagatg ctggcagatt atgcgccgtt gaataaagtc gacagcgctc
    42961 gtcgctgtgg cctctatttt ggtcgtctgg aaaaacagcc agtatttgat gcggataaat
    43021 tctggggcga atacagcgtc gatggcctga cggtcaaaac gctgcctggc gtgtttagcc
    43081 gcgacggtct ggatgtcggt agccagttgc tgctctcgac gttaactccg cacacgaaag
    43141 gtaaagtgct ggatgtcggc tgtggcgcgg gtgtactttc agttgccttt gcgcgccatt
    43201 cgccgaaaat tcgtctcacc ttgtgcgatg tctctgcccc ggcggtagaa gccagccgcg
    43261 caacacttgc agccaacggt gttgaaggtg aagtctttgc cagcaacgtc ttttccgagg
    43321 tgaaaggttg ttttgatatg atcatctcca acccgccgtt ccacgatggg atgcaaacca
    43381 gcctggatgc ggcgcaaacg ctgattcgtg gcgcggtgcg tcatcttaat agcggcggcg
    43441 agctgcgaat tgtagcgaac gccttcctgc cttacccgga cgtgctggat gagacatttg
    43501 gcttccatga agttatcgcg caaaccgggc gcttcaaggt atatcgcgcc attatgaccc
    43561 gccaggcgaa gaaaggttga gtatctgata tctgtcgccg cataccgtgt cggatgcggc
    43621 gtgaacgcct tatccgacct acaaaacctg cacgttagcc cttcgtaggc cagataagac
    43681 gcgccagcgt cgcatctggc atctgccccc gcatgcctac caaacctgca cgctagtttc
    43741 ctgatggaca tttttccagc aattacacat caggcgataa ttaactattg acgaaaagct
    43801 gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga tggtatgcga
    43861 aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg gacgtggggg
    43921 ttca
//
LOCUS       DADLCQ010000016        43876 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000016 DADLCQ010000000
VERSION     DADLCQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.016"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="JQG95_000815"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="JQG95_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY5887467.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="JQG95_000816"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="JQG95_000816"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY5887468.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3012..4091
                     /locus_tag="JQG95_000817"
     CDS             3012..4091
                     /locus_tag="JQG95_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY5887469.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="JQG95_000818"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="JQG95_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY5887470.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="JQG95_000819"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="JQG95_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY5887471.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="JQG95_000820"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="JQG95_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY5887472.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="JQG95_000821"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="JQG95_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY5887473.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="JQG95_000822"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="JQG95_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY5887474.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="JQG95_000823"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="JQG95_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY5887475.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="JQG95_000824"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="JQG95_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY5887476.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="JQG95_000825"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="JQG95_000825"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY5887477.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11837..13498
                     /locus_tag="JQG95_000826"
                     /pseudo
     CDS             11837..13498
                     /locus_tag="JQG95_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13558..15009)
                     /gene="djlC"
                     /locus_tag="JQG95_000827"
     CDS             complement(13558..15009)
                     /gene="djlC"
                     /locus_tag="JQG95_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY5887478.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15006..15713)
                     /locus_tag="JQG95_000828"
     CDS             complement(15006..15713)
                     /locus_tag="JQG95_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY5887479.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15815..16369
                     /locus_tag="JQG95_000829"
     CDS             15815..16369
                     /locus_tag="JQG95_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5887480.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLRWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16379..17806)
                     /locus_tag="JQG95_000830"
     CDS             complement(16379..17806)
                     /locus_tag="JQG95_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY5887481.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLSSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHEGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17803..18510)
                     /locus_tag="JQG95_000831"
     CDS             complement(17803..18510)
                     /locus_tag="JQG95_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY5887482.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18674..19651
                     /locus_tag="JQG95_000832"
     CDS             18674..19651
                     /locus_tag="JQG95_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY5887483.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19721..20203)
                     /locus_tag="JQG95_000833"
     CDS             complement(19721..20203)
                     /locus_tag="JQG95_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY5887484.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20438..23020
                     /gene="leuS"
                     /locus_tag="JQG95_000834"
     CDS             20438..23020
                     /gene="leuS"
                     /locus_tag="JQG95_000834"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY5887485.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23035..23616
                     /gene="lptE"
                     /locus_tag="JQG95_000835"
     CDS             23035..23616
                     /gene="lptE"
                     /locus_tag="JQG95_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY5887486.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23616..24647
                     /gene="holA"
                     /locus_tag="JQG95_000836"
     CDS             23616..24647
                     /gene="holA"
                     /locus_tag="JQG95_000836"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY5887487.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24649..25290
                     /gene="nadD"
                     /locus_tag="JQG95_000837"
     CDS             24649..25290
                     /gene="nadD"
                     /locus_tag="JQG95_000837"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY5887488.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25314..25916
                     /locus_tag="JQG95_000838"
     CDS             25314..25916
                     /locus_tag="JQG95_000838"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY5887489.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDIFATLRVLNSRAIGVENA"
     gene            26176..26493
                     /gene="rsfS"
                     /locus_tag="JQG95_000839"
                     /gene_synonym="rsfA"
     CDS             26176..26493
                     /gene="rsfS"
                     /locus_tag="JQG95_000839"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY5887490.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26497..26964
                     /gene="rlmH"
                     /locus_tag="JQG95_000840"
     CDS             26497..26964
                     /gene="rlmH"
                     /locus_tag="JQG95_000840"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY5887491.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            26995..28896
                     /gene="mrdA"
                     /locus_tag="JQG95_000841"
     CDS             26995..28896
                     /gene="mrdA"
                     /locus_tag="JQG95_000841"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY5887492.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28899..30011
                     /gene="mrdB"
                     /locus_tag="JQG95_000842"
                     /gene_synonym="rodA"
     CDS             28899..30011
                     /gene="mrdB"
                     /locus_tag="JQG95_000842"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY5887493.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30022..31110
                     /gene="rlpA"
                     /locus_tag="JQG95_000843"
     CDS             30022..31110
                     /gene="rlpA"
                     /locus_tag="JQG95_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY5887494.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPSVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31249..32460
                     /gene="dacA"
                     /locus_tag="JQG95_000844"
     CDS             31249..32460
                     /gene="dacA"
                     /locus_tag="JQG95_000844"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY5887495.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32571..32834
                     /locus_tag="JQG95_000845"
     CDS             32571..32834
                     /locus_tag="JQG95_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY5887496.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32935..33576
                     /gene="lipB"
                     /locus_tag="JQG95_000846"
     CDS             32935..33576
                     /gene="lipB"
                     /locus_tag="JQG95_000846"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY5887497.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33835..34788
                     /locus_tag="JQG95_000847"
     CDS             33835..34788
                     /locus_tag="JQG95_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY5887498.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            34997..35962
                     /gene="lipA"
                     /locus_tag="JQG95_000848"
     CDS             34997..35962
                     /gene="lipA"
                     /locus_tag="JQG95_000848"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY5887499.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36063..36266)
                     /gene="tatE"
                     /locus_tag="JQG95_000849"
     CDS             complement(36063..36266)
                     /gene="tatE"
                     /locus_tag="JQG95_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY5887500.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36395..37183)
                     /locus_tag="JQG95_000850"
     CDS             complement(36395..37183)
                     /locus_tag="JQG95_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY5887501.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37276..37659
                     /gene="crcB"
                     /locus_tag="JQG95_000851"
     CDS             37276..37659
                     /gene="crcB"
                     /locus_tag="JQG95_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY5887502.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37712..37921)
                     /gene="cspE"
                     /locus_tag="JQG95_000852"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37712..37921)
                     /gene="cspE"
                     /locus_tag="JQG95_000852"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY5887503.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38096..38656)
                     /locus_tag="JQG95_000853"
     CDS             complement(38096..38656)
                     /locus_tag="JQG95_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY5887504.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39244..40629
                     /gene="dcuC"
                     /locus_tag="JQG95_000854"
     CDS             39244..40629
                     /gene="dcuC"
                     /locus_tag="JQG95_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY5887505.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTDK"
     gene            complement(40670..41011)
                     /locus_tag="JQG95_000855"
                     /pseudo
     CDS             complement(40670..41011)
                     /locus_tag="JQG95_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41349..41858)
                     /locus_tag="JQG95_000856"
     CDS             complement(41349..41858)
                     /locus_tag="JQG95_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5887506.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42021..42266
                     /locus_tag="JQG95_000857"
     CDS             42021..42266
                     /locus_tag="JQG95_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5887507.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42241..43872
                     /locus_tag="JQG95_000858"
                     /pseudo
     CDS             42241..43872
                     /locus_tag="JQG95_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        11043 a        10967 c        10948 g        10918 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgccggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcattg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gcttttgcct cattaattga acgccagcgt
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aaccgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    10381 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    10441 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    10741 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    10861 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    11161 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    11341 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    11401 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    11701 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    11761 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    11821 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    11881 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    11941 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    12001 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    12061 aatgggcagc tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    12121 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    12181 gatctccgtt ccggcttatt tcagtgatga acaacgcaag catacccgtt tagcagcgga
    12241 cttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta
    12301 tggcctgcat acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt
    12361 tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctggcga
    12421 caacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    12481 ggatgtcaca aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    12541 agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc tggtagtacc aggatgaaac
    12601 gcgggaatgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    12661 cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga
    12721 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    12781 tctgtttggc aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc
    12841 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga
    12901 tatttgccct tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc
    12961 gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat
    13021 gcacccggaa caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    13081 aaacaacatt ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    13141 gattgatatt cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga
    13201 agacggcagc gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca
    13261 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat
    13321 caatcgcacc tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    13381 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc
    13441 ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc
    13501 acggctgccg gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca
    13561 actaaacaac atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat
    13621 tttgataatg ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt
    13681 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agttttactt tcgtcaacag
    13741 catccgggtg ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag
    13801 acgggccagc atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata
    13861 caaatccagt aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc
    13921 ccatgctttg cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    13981 tccgctatgt aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    14041 tttcagcggt tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa
    14101 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    14161 agatgggtaa ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca
    14221 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    14281 ccactgtttg gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg
    14341 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    14401 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    14461 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    14521 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    14581 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata
    14641 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    14701 ccaggtattt aattgctgaa taaatttctg ccaggcggag ggctgaaaac gatcactttc
    14761 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    14821 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    14881 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    14941 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    15001 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    15061 ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    15121 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    15181 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    15241 cagctgtgat agtaatggtg agctcgtgca tagacgcgcg attgcagcca taaactctct
    15301 tcttccgtca gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa
    15361 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    15421 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    15481 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    15541 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    15601 tcttttatcc cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    15661 atttccatgg gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa
    15721 taatccctta agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc
    15781 tttcaaaata aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt
    15841 cgcaattttt atagttgcag caattacctg tattagccaa cccaagaaaa cgatgttgcg
    15901 tgataaagcc atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt
    15961 aaccacggca gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct
    16021 tggcgaattt tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata
    16081 ctggtatgag caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tgaggtggat
    16141 ctacctgaaa ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga
    16201 agcagctgaa caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg
    16261 cactggtatt aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca
    16321 acattacaaa aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc
    16381 acccgaagag atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa
    16441 ttttgatgac gcccagacag cccagcccgc catcatcaac gggcattggc tgtggttgcg
    16501 gctccggctc gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa
    16561 taattaatgc ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta
    16621 atgattcttc tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc
    16681 ataagggatg ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct tcgtgcaaaa
    16741 ccatctcgac cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata
    16801 aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt
    16861 taaccagatc acgcgcttca atcatcatca ccactaatgg gtaataatag acccgatgct
    16921 gacggcacca ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt
    16981 aacaaagttc tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat
    17041 aataacgcgc atcttcattg tcaggttcag cttcccgcca cgaccgggcg taatccagga
    17101 gttcggggat tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg
    17161 catcatcggg gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat
    17221 gcgatgacca aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa
    17281 ctggcagatg cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa
    17341 aggattccag ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc
    17401 tttctgctaa taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc
    17461 acagcgacca gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga
    17521 atcgctgcca ggcggaggac agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg
    17581 cgagcagaat ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag
    17641 gcgactgcgc aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg
    17701 gatcggtttc tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga
    17761 cgtcagtcgt ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc
    17821 cttaagcgtt tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta
    17881 atacgcatca tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc
    17941 tttacgcgct aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata
    18001 ttgccgacca aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc
    18061 ccgtagatga atacgcgact ggatccatag actttcttct tcactcaacc agttattaag
    18121 tacgcccatt cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc
    18181 accacagcat tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa
    18241 ttcagcaaga tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc
    18301 tgccaaccga gcggcatgac cgttctcgat cattcggtga attatgcgca gcaggtcatc
    18361 acggtcatca attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt
    18421 gggggatgaa tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc
    18481 aaacaatatt ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga
    18541 atgtaaacta catttcgagg ctcagttatt tatacttttc tgacgcaaca taatacgcat
    18601 aaaataaggc ttacgcaaaa atggaataca taaaaatata atatatttta tctacaacca
    18661 taaggaaatg ataatgattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat
    18721 tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa
    18781 tcgcgatagc gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc
    18841 cgctgagcaa ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa
    18901 aagccgtcat aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg
    18961 ccatactttc gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta
    19021 taaagaagcg gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa
    19081 taatcttgga tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt
    19141 tttctggtac aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga
    19201 tctttataaa gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt
    19261 gaaaagcgca cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc
    19321 tggcgaaggg gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc
    19381 tcaggaaagc gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt
    19441 tgagaaagat tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc
    19501 cactgcctgg tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct
    19561 ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca
    19621 ggaaattcgt gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat
    19681 tctgcccata ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc
    19741 tctggaactg gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg
    19801 ggagatggaa gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt
    19861 gataaacccc atgatgattg aggtcctgga aaatttcgcg ccattcaagc tgcgttttat
    19921 cggtgatgtc tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt
    19981 cagattcttc tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga
    20041 cagctcgcgt cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct
    20101 cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca
    20161 gtgacgcgac cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt
    20221 cgttaactga ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa
    20281 ctctgtaaat taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc
    20341 tgttttgacg cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac
    20401 atttgtagcc gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga
    20461 agagatagaa tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga
    20521 agacgagagc aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact
    20581 acacatgggc cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat
    20641 gctgggcaaa aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg
    20701 cgcggcggtg aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat
    20761 gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg
    20821 tacgccggaa tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct
    20881 ggtatataaa aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa
    20941 cgaacaagtt atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat
    21001 cccgcagtgg tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa
    21061 actggatcac tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga
    21121 aggcgtggag atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac
    21181 ccgcccggac acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc
    21241 gcagaaagcg gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac
    21301 caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa
    21361 agctgttcac ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat
    21421 ggagtacggc acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt
    21481 tgcctctaaa tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc
    21541 agatctttct cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa
    21601 cggtcttgac catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt
    21661 tggcgagcgt aaagtgaact accgcctgcg cgactggggt gtttctcgcc agcgttactg
    21721 gggcgcgccg attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga
    21781 ccagctgccg gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa
    21841 agcagatccg gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga
    21901 cactttcgac acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta
    21961 caaagaaggt atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat
    22021 tggtggtatc gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat
    22081 gcgtgatgca ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat
    22141 ggtgctggca gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc
    22201 ggttgatgct atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc
    22261 aggccatgaa ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat
    22321 cgacccgcag gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt
    22381 tgcttctccg gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg
    22441 cttcctgaaa cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc
    22501 actgaacgtt gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac
    22561 tatcgctaaa gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc
    22621 gattatggag ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc
    22681 tctgatgcag gaagcactgc aggccgttgt ccgtatgctc aacccgttca ccccgcacat
    22741 ctgcttcacg ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc
    22801 ggttgctgac gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg
    22861 taaagtccgt gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg
    22921 tgctggccag gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat
    22981 ttacgtacca ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga
    23041 tatctggcaa cactgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat
    23101 ctgcgtgaca ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat
    23161 ccgaacgggc cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg
    23221 cttgataaag aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc
    23281 gcgaaagata ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg
    23341 acggtgaatg cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc
    23401 ttccgttcat tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg
    23461 atcgtaaaag agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc
    23521 cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca
    23581 acgcctgcac gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac
    23641 tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc
    23701 tgttattgca ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag
    23761 aacaccacac tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc
    23821 aggctatgag tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac
    23881 cgaatgcggc gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc
    23941 tattgatcgt ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg
    24001 ctcttgcgaa tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc
    24061 gctgggttgc tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg
    24121 tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat
    24181 cgctgctctg gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg
    24241 ccgcgcattt cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg
    24301 cgttgcatat tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca
    24361 cattacaacg tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac
    24421 tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg
    24481 cgttaaatcg cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg
    24541 aactcaccct caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc
    24601 ttctgttgtg ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta
    24661 caggctctgt ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa
    24721 acgctggcga atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg
    24781 catcgtcccc agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt
    24841 gccgacaagc cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac
    24901 actgcgcaaa cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt
    24961 attattggtc aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc
    25021 gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg
    25081 caataccagc aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag
    25141 cctgccggta aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc
    25201 cgcgaacgtt tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact
    25261 tacattaacc aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac
    25321 tgtggttaat tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg
    25381 cgcccacacc cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag
    25441 atgatgtttc ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc
    25501 gactggttct cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt
    25561 tttttggcga ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact
    25621 atagcgcgtg gtgcaatgac tggcagcatg ctatccccac gaatggtgaa ggatttcagg
    25681 cattttcgca acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga
    25741 atattttaat cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca
    25801 tgcctgccga gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata
    25861 tattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt
    25921 tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct
    25981 ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg
    26041 atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa
    26101 ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc
    26161 acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat
    26221 gacctcaaag gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac
    26281 tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt
    26341 gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc
    26401 gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt
    26461 cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg
    26521 ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg
    26581 aaagatatgc ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac
    26641 atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc
    26701 attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg
    26761 gaacgctgga agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg
    26821 tcgcctgcct gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc
    26881 catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc
    26941 aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa
    27001 ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg
    27061 gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg
    27121 caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg
    27181 cctatcgcgc ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac
    27241 cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg
    27301 gacgctttgc gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag
    27361 cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa
    27421 gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa
    27481 cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc
    27541 aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg
    27601 catgatattg gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc
    27661 ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta
    27721 ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt
    27781 gaaacgctgc tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg
    27841 gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc
    27901 agcaaagatt attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca
    27961 cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc
    28021 gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg
    28081 tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga
    28141 tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat
    28201 cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg
    28261 gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg
    28321 tggtatcagg gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca
    28381 atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat
    28441 ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg
    28501 cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt
    28561 gctaaccgcc ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg
    28621 gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac
    28681 aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat
    28741 ccgcaagtgg ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg
    28801 ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg
    28861 gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa
    28921 aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc
    28981 tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc
    29041 gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc
    29101 cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg
    29161 cggtagatgc tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg
    29221 ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta
    29281 tcaaccgcga cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat
    29341 ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc
    29401 tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag
    29461 tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc
    29521 gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca
    29581 ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg
    29641 gcactcagtc acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac
    29701 tggcggaaga gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga
    29761 tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg
    29821 gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta
    29881 ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg
    29941 tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga
    30001 aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga
    30061 atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct
    30121 gcggtatgta acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg
    30181 aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat
    30241 ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac
    30301 ctgacggcct ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt
    30361 ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc
    30421 attaatgatc gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct
    30481 gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag
    30541 gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc
    30601 ctgcctgcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag
    30661 ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg
    30721 gtaaccagta gcggtttcct cggcgctcca acgaccttag cgcctagtgt actggaaggc
    30781 agcgaaccga cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc
    30841 tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg
    30901 caagtcgggg ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag
    30961 aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc
    31021 ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa
    31081 ttacagtcat ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc
    31141 tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct
    31201 atagtagggc acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt
    31261 ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc
    31321 tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc
    31381 gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac
    31441 gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc
    31501 caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca
    31561 tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg
    31621 caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt
    31681 gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc
    31741 tacgttaacg cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct
    31801 gatggtcagt acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac
    31861 gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag
    31921 ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga
    31981 cacactgaca aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg
    32041 atttctgcgg tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg
    32101 ctaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc
    32161 gcctctgaac cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac
    32221 gtgtacctga ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac
    32281 agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag
    32341 cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt
    32401 aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa
    32461 aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact
    32521 cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca
    32581 aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt
    32641 tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca
    32701 ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg
    32761 ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc
    32821 gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc
    32881 tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat
    32941 caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccgggct
    33001 atgcatgaat tcaccgatac ccgcgataat agtacccttg atgaaatctg gctggtcgag
    33061 cactatccgg tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt
    33121 gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa
    33181 caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg
    33241 accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct
    33301 cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga
    33361 attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca
    33421 tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg
    33481 aaacccgaag cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta
    33541 ctaaacaatc cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt
    33601 acactgggtc attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat
    33661 tttctttatc acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat
    33721 taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat tagacataag
    33781 attattgtaa ttttttaaat attattttcc catgaataaa attggagtaa gtgcgtggat
    33841 agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata
    33901 tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta
    33961 cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag
    34021 tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt
    34081 gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc
    34141 attcttggcg cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg
    34201 accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat taaaactcac
    34261 tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag
    34321 tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat
    34381 gttctgttca ccgacaatat ggtgttaatt tgccgcaagg gaaatccact actctcttta
    34441 gaagatgaca gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagggcaa
    34501 aatttcagcg gtctgcggca gagagttcaa gagatgtttc cggaccggca aatcaatttc
    34561 accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt
    34621 attcccagcc gtttttataa cctgtttagt cgttgctggc cgctggaaaa actgcctttt
    34681 ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt catcctgcgt
    34741 gatcccatcc tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt
    34801 acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt
    34861 cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca
    34921 aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa
    34981 ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga
    35041 tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct
    35101 gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat
    35161 caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa
    35221 cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac
    35281 ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga
    35341 accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc
    35401 cgttgaccgt gatgacctgc gcgatggcgg tgcccaacac tttgcggatt gtattactgc
    35461 cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg
    35521 aatggatcgt gctctggata ttctgactgc aacgccccca gatgtgttca accataacct
    35581 ggaaaacgta ccgcgtattt accggcaggt acggcctggc gcagattaca actggtcgct
    35641 gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat
    35701 ggtgggtctg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca
    35761 tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt
    35821 tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg
    35881 ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc
    35941 gaaaggaatg gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc
    36001 cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca
    36061 cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc
    36121 gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg
    36181 cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc
    36241 agtttggtaa tactaatctc acccatagat accttcttga catataaccg gcaacagaac
    36301 gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg
    36361 taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc
    36421 gacggttgtt taagatgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt
    36481 ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta
    36541 taatccggct ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg
    36601 tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac
    36661 gaacccaggc ggcaggaagt accaggattt cagctccctg taaagcctgt gccagcgcca
    36721 gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca
    36781 gtaacggagc tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat
    36841 catagagatg cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca
    36901 tattccatgc gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc gtcatcatgt
    36961 tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt
    37021 taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca
    37081 gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt
    37141 cccacacaga tgtaacagca aactgtcctg cagcaaccag cattgccacc tcctgtcaga
    37201 gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa
    37261 tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga
    37321 agcgtagcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg
    37381 gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc
    37441 agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc
    37501 ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc
    37561 tttggctggg cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca
    37621 ctggcattct ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa acccgctgat
    37681 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    37741 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    37801 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    37861 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    37921 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc agtacaccaa
    37981 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    38041 gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    38101 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    38161 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    38221 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    38281 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    38341 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    38401 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    38461 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    38521 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc tcatctgcgt
    38581 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    38641 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    38701 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    38761 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    38821 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat gacaaaatgc
    38881 aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt aacgaagtat
    38941 ataatatata tcgtcaacca tgctcatatt agattatttt ttgagcgcac agccgcagct
    39001 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    39061 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    39121 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    39181 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    39241 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtggctcgc
    39301 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    39361 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    39421 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    39481 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    39541 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    39601 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    39661 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    39721 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    39781 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    39841 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    39901 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    39961 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    40021 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    40081 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    40141 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    40201 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    40261 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    40321 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    40381 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    40441 gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    40501 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    40561 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    40621 gacaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    40681 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    40741 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    40801 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    40861 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    40921 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    40981 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    41041 ccgggagaac attggattat tcgggatctg atgggattag atttggtggg gcttgcaagc
    41101 ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt
    41161 tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca tagcttcaaa
    41221 aaacgttcaa aaccgataac aaccaggctg tcaccaaatg actcatatca caaatcagct
    41281 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggct
    41341 catggttgtt atacggcttt tttcgcatct tgtggttcca acaaaggagc tacaggctct
    41401 tcctgtttat gaacaccata cattgacaaa atctcttggc gagtttctgg aggtaattta
    41461 tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac ccagcgttgc caatagggtg
    41521 tcaacggaga tggaggggcg ttcttgcatt aatccggaaa gcccatattt agccatgttt
    41581 agctctatct ctcggacttc attttctcct agtttattga ttaacaggga gaacattttc
    41641 aatgtatcag cattcatttc ttcgatagca attttttgca tagccggtat tgccgggata
    41701 tattcaaccc caggcccgcg acgcccttct tgtgaacgct tcggccatcc ttctttcttt
    41761 gctcgtttat gcaccccctg tattgtccca ggcattccat ccagttcagt aatttgtttg
    41821 ggtgtcatcc actggtttat ctcatttgcg cttttcataa actaagttta ccagatttat
    41881 ctatataaaa atcaatggat tacaaaataa accacattaa ctgatataaa ccggttttgt
    41941 ttatttggta atgtataaaa cagataatca agacgaatta tttgttttgg taacccttta
    42001 tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga ttggcatcgg gccgacgtga
    42061 ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt gtccagacgt tcaggttatt
    42121 caaccgggac gcttgcaaat gcattagtca ggccatggcc taaaggtgaa cgcattattg
    42181 ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag tagatatcta agggatcaca
    42241 atgagagagt ggatatcagc aattgaatgc atgggtttat ctggtttacc tggttcatta
    42301 atgggggttc acaaaagagc tcagagagaa ggatggctca agagaagaag agaaggaatt
    42361 agaggggttg cttacgaata ccatatatct tctcttccta acattgctcg agctgaagtt
    42421 ttgctccaac aaggaaaatt taaaaccagt cagggatgtt ttgaaatcgc ccgccccacg
    42481 ctggaagctc atgattatga tcgtgaggca ctatggagca aatgggataa agccagcgat
    42541 tcccagcgca gcctggctga aaaatggttg ccctcaattc aggccacaga cgaaatgctg
    42601 aaccagggga tttcaacgaa aacggctttt gcgaccgttg cagggcatta ccaggtcagc
    42661 gcatccactt tgcgggacaa gtattaccag gtacagaagt ttgcgaagcc tgactgggcg
    42721 gctgcacttg ttgatggacg tggagcatcc cgtagcaatg ttcacaaaag tgaatttgac
    42781 gaggatgcct ggcagtttct gattgcagat tatctgcgac cggaaaaacc cgccttccgc
    42841 aaatgttatg agcgtctgga actggcagcc cgcgagcatg gctggagtat tccctcccgt
    42901 gccacggcct ttcgccggat tcagcaactg gacgaggcaa tggttgttgc ctgtcgtgaa
    42961 ggtgaacatg cactgatgca tctgataccg gcacagcagc gaactgtgga acacctggac
    43021 gccatgcagt ggatcaacgg cgacggttat ctgcataacg tctttgtacg ctggtttaac
    43081 ggtgatgtga tccgtccgaa aacatggttc tggcaggatg tgaaaacccg aaaaattctg
    43141 ggctggcgct gcgatgtgag cgagaacatt gattcaattc gcctctcgtt catggatgtt
    43201 gtgactcgct acagtatccc ggaggatttt cacatcacca ttgataacac ccgtggtgct
    43261 gcgaataaat ggctgacggg aggcgcgccc aatcgctacc gctttaaggt aaaagaggac
    43321 gatccaaaag gactgttttt actgatgggt gcgaaaatgc actggacaag cgttgttgcc
    43381 ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt tcggtgttgg tgggcttgag
    43441 gaatacgttg ataagcatcc ggcactggct ggcgcatata cggggccaaa tccgcaggca
    43501 aaacctgata actatggcga ccacgctgtt gatgcagagc tgtttctgaa aacctttgcc
    43561 gaaggtgtgg cgatgttcaa tgccagaaca ggccgtgaaa cagaaatgtg cgggggcaaa
    43621 ctctcgtttg atgatgtttt cgagcgtgaa tacgccagaa cgattgtgcg taagccaacc
    43681 gaagaacaaa aacggatgct gttactgcct gccgaggcgg tgaacgtttc acgcaaaggc
    43741 gagtttacgc ttaaagttgg cggctccctt aaaggcgcga aaaacgttta ttacaacatg
    43801 gcattaatga atgccggagt gaaaaaagtt gtggtcagat ttgatccaca gcagttacac
    43861 agcacggttt attgag
//
LOCUS       DADLCQ010000017        43559 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000017 DADLCQ010000000
VERSION     DADLCQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.017"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1405)
                     /locus_tag="JQG95_000859"
     CDS             complement(<1..1405)
                     /locus_tag="JQG95_000859"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY5887508.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRG"
     gene            1760..3613
                     /locus_tag="JQG95_000860"
                     /pseudo
     CDS             1760..3613
                     /locus_tag="JQG95_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(3684..5108)
                     /gene="lpdA"
                     /locus_tag="JQG95_000861"
     CDS             complement(3684..5108)
                     /gene="lpdA"
                     /locus_tag="JQG95_000861"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY5887509.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(5433..7313)
                     /gene="aceF"
                     /locus_tag="JQG95_000862"
     CDS             complement(5433..7313)
                     /gene="aceF"
                     /locus_tag="JQG95_000862"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY5887510.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(7328..9991)
                     /gene="aceE"
                     /locus_tag="JQG95_000863"
     CDS             complement(7328..9991)
                     /gene="aceE"
                     /locus_tag="JQG95_000863"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY5887511.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(10152..10916)
                     /gene="pdhR"
                     /locus_tag="JQG95_000864"
     CDS             complement(10152..10916)
                     /gene="pdhR"
                     /locus_tag="JQG95_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY5887512.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            11457..12830
                     /gene="aroP"
                     /locus_tag="JQG95_000865"
     CDS             11457..12830
                     /gene="aroP"
                     /locus_tag="JQG95_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY5887513.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWTMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(12873..13727)
                     /gene="ampE"
                     /locus_tag="JQG95_000866"
     CDS             complement(12873..13727)
                     /gene="ampE"
                     /locus_tag="JQG95_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY5887514.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(13724..14275)
                     /gene="ampD"
                     /locus_tag="JQG95_000867"
     CDS             complement(13724..14275)
                     /gene="ampD"
                     /locus_tag="JQG95_000867"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY5887515.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            14363..15256
                     /gene="nadC"
                     /locus_tag="JQG95_000868"
     CDS             14363..15256
                     /gene="nadC"
                     /locus_tag="JQG95_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY5887516.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            15459..15899
                     /gene="ppdD"
                     /locus_tag="JQG95_000869"
     CDS             15459..15899
                     /gene="ppdD"
                     /locus_tag="JQG95_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY5887517.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            15909..17056
                     /gene="cpaF"
                     /locus_tag="JQG95_000870"
                     /pseudo
     CDS             15909..17056
                     /gene="cpaF"
                     /locus_tag="JQG95_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
     gene            17046..18248
                     /gene="hofC"
                     /locus_tag="JQG95_000871"
     CDS             17046..18248
                     /gene="hofC"
                     /locus_tag="JQG95_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY5887518.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(18283..19326)
                     /gene="guaC"
                     /locus_tag="JQG95_000872"
     CDS             complement(18283..19326)
                     /gene="guaC"
                     /locus_tag="JQG95_000872"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY5887519.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            19551..20171
                     /gene="coaE"
                     /locus_tag="JQG95_000873"
     CDS             19551..20171
                     /gene="coaE"
                     /locus_tag="JQG95_000873"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY5887520.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            20171..20914
                     /gene="zapD"
                     /locus_tag="JQG95_000874"
     CDS             20171..20914
                     /gene="zapD"
                     /locus_tag="JQG95_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY5887521.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            20924..21121
                     /gene="yacG"
                     /locus_tag="JQG95_000875"
     CDS             20924..21121
                     /gene="yacG"
                     /locus_tag="JQG95_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY5887522.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(21221..21610)
                     /gene="mutT"
                     /locus_tag="JQG95_000876"
     CDS             complement(21221..21610)
                     /gene="mutT"
                     /locus_tag="JQG95_000876"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY5887523.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(21670..24375)
                     /gene="secA"
                     /locus_tag="JQG95_000877"
     CDS             complement(21670..24375)
                     /gene="secA"
                     /locus_tag="JQG95_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY5887524.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(24437..24949)
                     /gene="secM"
                     /locus_tag="JQG95_000878"
     CDS             complement(24437..24949)
                     /gene="secM"
                     /locus_tag="JQG95_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY5887525.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(25180..26097)
                     /gene="lpxC"
                     /locus_tag="JQG95_000879"
     CDS             complement(25180..26097)
                     /gene="lpxC"
                     /locus_tag="JQG95_000879"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY5887526.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(26198..27349)
                     /gene="ftsZ"
                     /locus_tag="JQG95_000880"
     CDS             complement(26198..27349)
                     /gene="ftsZ"
                     /locus_tag="JQG95_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY5887527.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(27410..28672)
                     /gene="ftsA"
                     /locus_tag="JQG95_000881"
     CDS             complement(27410..28672)
                     /gene="ftsA"
                     /locus_tag="JQG95_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY5887528.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(28669..29499)
                     /gene="ftsQ"
                     /locus_tag="JQG95_000882"
     CDS             complement(28669..29499)
                     /gene="ftsQ"
                     /locus_tag="JQG95_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY5887529.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(29501..30421)
                     /locus_tag="JQG95_000883"
     CDS             complement(29501..30421)
                     /locus_tag="JQG95_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY5887530.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(30414..31889)
                     /gene="murC"
                     /locus_tag="JQG95_000884"
     CDS             complement(30414..31889)
                     /gene="murC"
                     /locus_tag="JQG95_000884"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY5887531.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(31943..33010)
                     /gene="murG"
                     /locus_tag="JQG95_000885"
     CDS             complement(31943..33010)
                     /gene="murG"
                     /locus_tag="JQG95_000885"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY5887532.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(33007..34251)
                     /gene="ftsW"
                     /locus_tag="JQG95_000886"
     CDS             complement(33007..34251)
                     /gene="ftsW"
                     /locus_tag="JQG95_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY5887533.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(34251..35567)
                     /gene="murD"
                     /locus_tag="JQG95_000887"
     CDS             complement(34251..35567)
                     /gene="murD"
                     /locus_tag="JQG95_000887"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY5887534.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(35570..36652)
                     /gene="mraY"
                     /locus_tag="JQG95_000888"
     CDS             complement(35570..36652)
                     /gene="mraY"
                     /locus_tag="JQG95_000888"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY5887535.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(36646..38004)
                     /gene="murF"
                     /locus_tag="JQG95_000889"
     CDS             complement(36646..38004)
                     /gene="murF"
                     /locus_tag="JQG95_000889"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY5887536.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(38001..39488)
                     /gene="murE"
                     /locus_tag="JQG95_000890"
     CDS             complement(38001..39488)
                     /gene="murE"
                     /locus_tag="JQG95_000890"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY5887537.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSCVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(39475..41241)
                     /locus_tag="JQG95_000891"
     CDS             complement(39475..41241)
                     /locus_tag="JQG95_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY5887538.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKESMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(41257..41622)
                     /gene="ftsL"
                     /locus_tag="JQG95_000892"
     CDS             complement(41257..41622)
                     /gene="ftsL"
                     /locus_tag="JQG95_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY5887539.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(41619..42560)
                     /gene="rsmH"
                     /locus_tag="JQG95_000893"
     CDS             complement(41619..42560)
                     /gene="rsmH"
                     /locus_tag="JQG95_000893"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY5887540.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(42562..43020)
                     /gene="mraZ"
                     /locus_tag="JQG95_000894"
     CDS             complement(42562..43020)
                     /gene="mraZ"
                     /locus_tag="JQG95_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY5887541.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        10273 a        12180 c        10764 g        10342 t
ORIGIN      
        1 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
       61 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
      121 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
      181 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
      241 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
      301 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
      361 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
      421 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
      481 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
      541 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
      601 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
      661 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
      721 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
      781 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
      841 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
      901 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
      961 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
     1021 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
     1081 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
     1141 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
     1201 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
     1261 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
     1321 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
     1381 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
     1441 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
     1501 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
     1561 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
     1621 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
     1681 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
     1741 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
     1801 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
     1861 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
     1921 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
     1981 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
     2041 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
     2101 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
     2161 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
     2221 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
     2281 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
     2341 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
     2401 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
     2461 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
     2521 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
     2581 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
     2641 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
     2701 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
     2761 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
     2821 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
     2881 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
     2941 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
     3001 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
     3061 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
     3121 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
     3181 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
     3241 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
     3301 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
     3361 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
     3421 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
     3481 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
     3541 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
     3601 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
     3661 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
     3721 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
     3781 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
     3841 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
     3901 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
     3961 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
     4021 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
     4081 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
     4141 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
     4201 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
     4261 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
     4321 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
     4381 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
     4441 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
     4501 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
     4561 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
     4621 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
     4681 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
     4741 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
     4801 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
     4861 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
     4921 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
     4981 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
     5041 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
     5101 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
     5161 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
     5221 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
     5281 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
     5341 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
     5401 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
     5461 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
     5521 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
     5581 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
     5641 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
     5701 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
     5761 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
     5821 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
     5881 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
     5941 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
     6001 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
     6061 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
     6121 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
     6181 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
     6241 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
     6301 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
     6361 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
     6421 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
     6481 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
     6541 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
     6601 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
     6661 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
     6721 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
     6781 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
     6841 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
     6901 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
     6961 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
     7021 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
     7081 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
     7141 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
     7201 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
     7261 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
     7321 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
     7381 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
     7441 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
     7501 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
     7561 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
     7621 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
     7681 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
     7741 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
     7801 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
     7861 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
     7921 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
     7981 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
     8041 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
     8101 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
     8161 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
     8221 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
     8281 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
     8341 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
     8401 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
     8461 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
     8521 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
     8581 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
     8641 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
     8701 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
     8761 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
     8821 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
     8881 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
     8941 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
     9001 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
     9061 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
     9121 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
     9181 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
     9241 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
     9301 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
     9361 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
     9421 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
     9481 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
     9541 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
     9601 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
     9661 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
     9721 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
     9781 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
     9841 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
     9901 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
     9961 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    10021 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    10081 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    10141 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    10201 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    10261 tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta
    10321 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    10381 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    10441 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    10501 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    10561 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    10621 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    10681 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    10741 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    10801 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    10861 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    10921 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    10981 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    11041 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    11101 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    11161 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    11221 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    11281 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    11341 ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc
    11401 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    11461 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    11521 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    11581 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    11641 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    11701 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    11761 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    11821 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    11881 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    11941 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    12001 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    12061 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    12121 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    12181 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    12241 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    12301 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    12361 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    12421 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    12481 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    12541 taatggcact ggtggtatcc gcactggtga tcaactggac gatgatcagt ctggcgcata
    12601 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    12661 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    12721 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    12781 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    12841 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    12901 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    12961 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    13021 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    13081 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    13141 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    13201 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    13261 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    13321 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    13381 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    13441 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    13501 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    13561 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    13621 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    13681 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    13741 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    13801 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    13861 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    13921 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    13981 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    14041 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    14101 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    14161 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    14221 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    14281 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    14341 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    14401 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    14461 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    14521 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    14581 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    14641 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    14701 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    14761 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    14821 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    14881 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    14941 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    15001 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    15061 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    15121 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    15181 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    15241 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    15301 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    15361 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    15421 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    15481 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    15541 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    15601 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    15661 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    15721 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    15781 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    15841 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    15901 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    15961 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    16021 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    16081 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    16141 atcagcccaa agcagaattg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    16201 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    16261 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    16321 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    16381 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    16441 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    16501 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    16561 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    16621 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    16681 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    16741 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    16801 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    16861 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    16921 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    16981 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    17041 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    17101 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    17161 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    17221 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    17281 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    17341 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    17401 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    17461 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    17521 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    17581 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    17641 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    17701 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    17761 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    17821 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    17881 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    17941 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    18001 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    18061 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    18121 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    18181 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    18241 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    18301 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    18361 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    18421 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    18481 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    18541 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    18601 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    18661 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    18721 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    18781 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    18841 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    18901 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    18961 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    19021 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    19081 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    19141 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    19201 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    19261 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    19321 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    19381 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    19441 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    19501 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    19561 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    19621 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    19681 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    19741 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    19801 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    19861 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    19921 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    19981 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    20041 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    20101 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    20161 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    20221 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    20281 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    20341 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    20401 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    20461 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    20521 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    20581 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    20641 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    20701 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    20761 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    20821 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    20881 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    20941 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    21001 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    21061 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    21121 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    21181 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    21241 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    21301 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    21361 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    21421 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    21481 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    21541 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    21601 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    21661 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    21721 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    21781 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    21841 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    21901 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    21961 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    22021 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    22081 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    22141 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    22201 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    22261 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    22321 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    22381 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    22441 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    22501 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    22561 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    22621 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    22681 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    22741 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    22801 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    22861 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    22921 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    22981 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    23041 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    23101 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    23161 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    23221 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    23281 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    23341 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    23401 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    23461 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    23521 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    23581 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    23641 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    23701 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    23761 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    23821 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    23881 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    23941 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    24001 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    24061 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    24121 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    24181 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    24241 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    24301 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    24361 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    24421 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    24481 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    24541 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    24601 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    24661 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    24721 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    24781 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    24841 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    24901 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    24961 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    25021 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    25081 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    25141 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    25201 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    25261 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    25321 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    25381 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    25441 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    25501 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    25561 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    25621 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    25681 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    25741 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    25801 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    25861 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    25921 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    25981 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    26041 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    26101 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    26161 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    26221 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    26281 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    26341 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    26401 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    26461 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    26521 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    26581 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    26641 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    26701 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    26761 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    26821 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    26881 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    26941 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    27001 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    27061 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    27121 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    27181 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    27241 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    27301 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    27361 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    27421 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    27481 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    27541 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    27601 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    27661 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    27721 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    27781 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    27841 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    27901 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    27961 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    28021 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    28081 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    28141 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    28201 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    28261 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    28321 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    28381 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    28441 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    28501 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    28561 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    28621 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    28681 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    28741 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    28801 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    28861 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    28921 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    28981 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    29041 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    29101 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    29161 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    29221 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    29281 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    29341 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    29401 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    29461 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    29521 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    29581 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    29641 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    29701 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    29761 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    29821 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    29881 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    29941 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    30001 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    30061 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    30121 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    30181 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    30241 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    30301 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    30361 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    30421 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    30481 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    30541 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    30601 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    30661 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    30721 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    30781 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    30841 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    30901 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    30961 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    31021 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct gacgcagcag cgtaaagtgc
    31081 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    31141 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    31201 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    31261 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    31321 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    31381 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    31441 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    31501 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    31561 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    31621 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    31681 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    31741 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    31801 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    31861 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    31921 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    31981 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    32041 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcaa
    32101 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    32161 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    32221 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    32281 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    32341 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    32401 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    32461 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    32521 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    32581 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    32641 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    32701 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    32761 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    32821 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    32881 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    32941 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    33001 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    33061 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    33121 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    33181 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    33241 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    33301 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    33361 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    33421 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    33481 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    33541 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    33601 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    33661 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    33721 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    33781 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    33841 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    33901 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    33961 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    34021 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    34081 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    34141 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    34201 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    34261 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    34321 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    34381 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    34441 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    34501 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    34561 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    34621 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    34681 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    34741 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    34801 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    34861 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    34921 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    34981 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    35041 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    35101 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    35161 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    35221 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    35281 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    35341 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    35401 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    35461 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    35521 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    35581 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    35641 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    35701 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    35761 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    35821 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    35881 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    35941 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    36001 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    36061 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    36121 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    36181 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    36241 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    36301 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    36361 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    36421 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    36481 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    36541 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    36601 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    36661 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    36721 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    36781 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    36841 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    36901 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    36961 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    37021 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    37081 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    37141 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    37201 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    37261 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    37321 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    37381 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    37441 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    37501 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    37561 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    37621 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    37681 ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    37741 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    37801 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    37861 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    37921 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    37981 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    38041 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    38101 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    38161 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    38221 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    38281 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    38341 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    38401 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    38461 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    38521 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    38581 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    38641 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    38701 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    38761 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    38821 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    38881 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    38941 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    39001 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    39061 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    39121 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    39181 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    39241 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    39301 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    39361 cctactacag ctacaaagag atcgcccgcc gcagccacac agctgtcgag tgtcatctct
    39421 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    39481 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    39541 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    39601 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    39661 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    39721 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    39781 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    39841 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    39901 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    39961 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    40021 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    40081 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    40141 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    40201 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    40261 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    40321 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgactctt tcggcgtgcc
    40381 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    40441 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    40501 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    40561 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    40621 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    40681 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    40741 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    40801 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    40861 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    40921 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    40981 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    41041 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    41101 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    41161 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    41221 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    41281 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    41341 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    41401 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    41461 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    41521 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    41581 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    41641 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    41701 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    41761 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    41821 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    41881 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    41941 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    42001 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    42061 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    42121 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    42181 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    42241 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    42301 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    42361 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    42421 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    42481 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    42541 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    42601 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    42661 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    42721 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    42781 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    42841 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    42901 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    42961 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    43021 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    43081 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    43141 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    43201 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    43261 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    43321 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    43381 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    43441 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    43501 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DADLCQ010000018        43110 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000018 DADLCQ010000000
VERSION     DADLCQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.018"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..228
                     /locus_tag="JQG95_000895"
     CDS             <1..228
                     /locus_tag="JQG95_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887542.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            771..1263
                     /locus_tag="JQG95_000896"
                     /pseudo
     CDS             771..1263
                     /locus_tag="JQG95_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="JQG95_000897"
     CDS             1366..1689
                     /locus_tag="JQG95_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HAY5887543.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="JQG95_000898"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="JQG95_000898"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY5887544.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="JQG95_000899"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="JQG95_000899"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY5887545.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="JQG95_000900"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="JQG95_000900"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY5887546.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="JQG95_000901"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="JQG95_000901"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY5887547.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="JQG95_000902"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="JQG95_000902"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY5887548.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="JQG95_000903"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="JQG95_000903"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY5887549.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="JQG95_000904"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="JQG95_000904"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY5887550.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="JQG95_000905"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="JQG95_000905"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY5887551.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="JQG95_000906"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="JQG95_000906"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY5887552.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="JQG95_000907"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="JQG95_000907"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY5887553.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="JQG95_000908"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="JQG95_000908"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY5887554.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /locus_tag="JQG95_000909"
     CDS             complement(15659..16321)
                     /locus_tag="JQG95_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY5887555.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="JQG95_000910"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="JQG95_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY5887556.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="JQG95_000911"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="JQG95_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY5887557.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19269..20486
                     /locus_tag="JQG95_000912"
     CDS             19269..20486
                     /locus_tag="JQG95_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY5887558.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20570..21169
                     /gene="yfbR"
                     /locus_tag="JQG95_000913"
     CDS             20570..21169
                     /gene="yfbR"
                     /locus_tag="JQG95_000913"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY5887559.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21228..23060)
                     /locus_tag="JQG95_000914"
     CDS             complement(21228..23060)
                     /locus_tag="JQG95_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY5887560.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="JQG95_000915"
     CDS             complement(23147..23797)
                     /gene="hxpA"
                     /locus_tag="JQG95_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY5887561.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23808..24302)
                     /locus_tag="JQG95_000916"
     CDS             complement(23808..24302)
                     /locus_tag="JQG95_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY5887562.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24385..24840)
                     /locus_tag="JQG95_000917"
     CDS             complement(24385..24840)
                     /locus_tag="JQG95_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY5887563.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25178..26380
                     /gene="ackA"
                     /locus_tag="JQG95_000918"
     CDS             25178..26380
                     /gene="ackA"
                     /locus_tag="JQG95_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY5887564.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26455..28599
                     /gene="pta"
                     /locus_tag="JQG95_000919"
     CDS             26455..28599
                     /gene="pta"
                     /locus_tag="JQG95_000919"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY5887565.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28789..30309
                     /gene="yfcC"
                     /locus_tag="JQG95_000920"
     CDS             28789..30309
                     /gene="yfcC"
                     /locus_tag="JQG95_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY5887566.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="JQG95_000921"
     CDS             complement(30342..30884)
                     /gene="yfcD"
                     /locus_tag="JQG95_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY5887567.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="JQG95_000922"
     CDS             complement(30942..31493)
                     /gene="yfcE"
                     /locus_tag="JQG95_000922"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY5887568.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="JQG95_000923"
     CDS             complement(31549..32193)
                     /gene="yfcF"
                     /locus_tag="JQG95_000923"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY5887569.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32329..32976
                     /gene="yfcG"
                     /locus_tag="JQG95_000924"
     CDS             32329..32976
                     /gene="yfcG"
                     /locus_tag="JQG95_000924"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY5887570.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33033..33395
                     /gene="folX"
                     /locus_tag="JQG95_000925"
     CDS             33033..33395
                     /gene="folX"
                     /locus_tag="JQG95_000925"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY5887571.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33416..34309
                     /locus_tag="JQG95_000926"
     CDS             33416..34309
                     /locus_tag="JQG95_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY5887572.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34357..35247)
                     /locus_tag="JQG95_000927"
     CDS             complement(34357..35247)
                     /locus_tag="JQG95_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY5887573.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="JQG95_000928"
     CDS             complement(35444..36217)
                     /gene="hisP"
                     /locus_tag="JQG95_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY5887574.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36225..36941)
                     /locus_tag="JQG95_000929"
     CDS             complement(36225..36941)
                     /locus_tag="JQG95_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5887575.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36938..37624)
                     /locus_tag="JQG95_000930"
     CDS             complement(36938..37624)
                     /locus_tag="JQG95_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5887576.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="JQG95_000931"
     CDS             complement(37714..38496)
                     /gene="hisJ"
                     /locus_tag="JQG95_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY5887577.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38717..39499)
                     /gene="argT"
                     /locus_tag="JQG95_000932"
     CDS             complement(38717..39499)
                     /gene="argT"
                     /locus_tag="JQG95_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY5887578.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39765..40334)
                     /locus_tag="JQG95_000933"
     CDS             complement(39765..40334)
                     /locus_tag="JQG95_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY5887579.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40429..41946)
                     /gene="purF"
                     /locus_tag="JQG95_000934"
     CDS             complement(40429..41946)
                     /gene="purF"
                     /locus_tag="JQG95_000934"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY5887580.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="JQG95_000935"
     CDS             complement(41983..42471)
                     /gene="cvpA"
                     /locus_tag="JQG95_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY5887581.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42730..>43110)
                     /gene="dedD"
                     /locus_tag="JQG95_000936"
     CDS             complement(42730..>43110)
                     /gene="dedD"
                     /locus_tag="JQG95_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY5887582.1"
                     /translation="TEFEPEPAPVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAA
                     PTGKAYVVQLGALKNADKVNEIVGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDK
                     LKGSLGELKQLSGLSGVVMGYTPN"
BASE COUNT        10893 a        11626 c        10668 g         9923 t
ORIGIN      
        1 gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa
       61 aaatattcag cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat
      121 tgttatttag ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata
      181 aaacactcac aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa
      241 tcaattcgac cagtcagtaa atatgctgac tggtcgaaca taatttgcta aaaagtcatc
      301 actgactgat gtcagtccca ccatctgcca gttcaatcta cgggtatttc cagcatatcg
      361 cggcacgcga ttgctggtct gttgatgtag tggattgtag ttgctgactt ttcttaccct
      421 cagatttctg ccccacttgt caattcaatc tgccaataaa taacataatt atatttttat
      481 tcctcacaga aattcctttt taaaaatagc gaacccactg taaaaaacac tttttgtata
      541 cctctaccaa gcgtaataaa ataaacgctc ataattcatt ataaataata atgaaataac
      601 gccttttatt gataatttac atatgaaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctataaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa aaaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac
    19141 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
    19201 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
    19261 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
    19321 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
    19381 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
    19441 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
    19501 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
    19561 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
    19621 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
    19681 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
    19741 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa
    19801 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
    19861 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
    19921 ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac
    19981 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
    20041 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
    20101 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
    20161 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
    20221 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
    20281 tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc
    20341 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
    20401 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
    20461 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
    20521 tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
    20581 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
    20641 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
    20701 ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact
    20761 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
    20821 cttcaatgcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact
    20881 ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc
    20941 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
    21001 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
    21061 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
    21121 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
    21181 ctgcgtcgca tcagtcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
    21241 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
    21301 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa
    21361 gacaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa
    21421 ggcgacactc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
    21481 gcggtattag agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc
    21541 atatgtggcc cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct
    21601 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
    21661 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
    21721 caggcgatga tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt
    21781 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
    21841 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
    21901 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
    21961 ccttccagcg ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga
    22021 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
    22081 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
    22141 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
    22201 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
    22261 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
    22321 tccagccgtt gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
    22381 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
    22441 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
    22501 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
    22561 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
    22621 ctaatcagcc cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc
    22681 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac
    22741 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
    22801 gccactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
    22861 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
    22921 tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
    22981 tccatacgca ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc
    23041 caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc
    23101 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
    23161 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
    23221 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg
    23281 ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa
    23341 gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc
    23401 gctcagcggt tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg
    23461 ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat
    23521 tgagtaaggc gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt
    23581 gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc
    23641 gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc
    23701 catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg
    23761 ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata
    23821 atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta
    23881 agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg
    23941 tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt
    24001 gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg
    24061 tcctgcaaat tagaccagga aacatacaac gcatgataca tctccataat gtcgatgata
    24121 gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat
    24181 ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg
    24241 agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc
    24301 attgttgact cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct
    24361 tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt
    24421 gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc
    24481 caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc
    24541 agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact
    24601 taaggcgaac agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca
    24661 gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat
    24721 aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta
    24781 atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat
    24841 gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg
    24901 gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga
    24961 aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg
    25021 aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc
    25081 agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg
    25141 acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt
    25201 tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga
    25261 gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat
    25321 ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa
    25381 ctttatcgtt aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat
    25441 cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc
    25501 tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct
    25561 gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt
    25621 atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta
    25681 caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta
    25741 tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac
    25801 ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac
    25861 ctctatgggt ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga
    25921 tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa
    25981 actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta
    26041 tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca
    26101 ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt
    26161 tgcattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa
    26221 actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa
    26281 atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga
    26341 actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc
    26401 tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc
    26461 cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc
    26521 gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag
    26581 ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc
    26641 accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat
    26701 cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct
    26761 gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg
    26821 aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact
    26881 gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc
    26941 aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag
    27001 ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac
    27061 aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg
    27121 tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc
    27181 atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc
    27241 agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac
    27301 cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc
    27361 ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct
    27421 ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc
    27481 ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag
    27541 gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag
    27601 cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa
    27661 gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct
    27721 atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac
    27781 cgtgttgcag cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa
    27841 gtggttcgcg aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc
    27901 gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa
    27961 caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt
    28021 ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc
    28081 atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc
    28141 gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc
    28201 gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta
    28261 gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac
    28321 ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac
    28381 tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc
    28441 acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt
    28501 atgcgcaaac cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc
    28561 atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt
    28621 tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt
    28681 tgcattaatc ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct
    28741 cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc
    28801 actgaatcca aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt
    28861 gttgctattt taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag
    28921 gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca
    28981 tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg
    29041 acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg
    29101 aaatacggga cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc
    29161 attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc
    29221 gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta
    29281 tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg
    29341 ctgggctatg acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt
    29401 gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg
    29461 gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg
    29521 atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat
    29581 gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt
    29641 ggtgactggc tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg
    29701 atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg
    29761 attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca
    29821 tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa
    29881 gggattttgc tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc
    29941 ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg
    30001 ttgctcttcc aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta
    30061 actatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg
    30121 ctggcgttcc agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta
    30181 atggcaacgc tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc
    30241 ctgcttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc
    30301 taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc
    30361 tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct
    30421 ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca aactccactg
    30481 acttcatctt cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag
    30541 acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca
    30601 atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga
    30661 actacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga
    30721 cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc
    30781 agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg
    30841 acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct
    30901 ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc
    30961 tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg
    31021 ccataacccg ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa
    31081 atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc
    31141 tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt
    31201 tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga
    31261 tccacttcgc tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc
    31321 cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc
    31381 aacacgtcgc caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca
    31441 cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac
    31501 tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg
    31561 cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata
    31621 atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc
    31681 cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa
    31741 atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc
    31801 ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc
    31861 gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata
    31921 aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga
    31981 ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc
    32041 ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga
    32101 caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc
    32161 atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg
    32221 ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg
    32281 cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc
    32341 tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt
    32401 gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg
    32461 cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc
    32521 gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga
    32581 ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag
    32641 gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa
    32701 accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta
    32761 aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt
    32821 gcctgctggc cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg
    32881 gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta
    32941 aaagcacaac tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat
    33001 ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta
    33061 taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc
    33121 aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag
    33181 atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga
    33241 ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac
    33301 atcactgggt gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg
    33361 attcggtatc catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa
    33421 catagtgatc accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga
    33481 gctgggccat caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg
    33541 ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc
    33601 ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga
    33661 gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga ttaatgctag
    33721 cgacacgccg ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg
    33781 tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc
    33841 ccgctgggaa gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg
    33901 aaccggcgtg gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg
    33961 tcttggcctg ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga
    34021 tgatatggtc aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat
    34081 ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca
    34141 tcgcccggct attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc
    34201 agtactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc
    34261 gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca
    34321 gcaaacatcc gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc
    34381 gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat
    34441 ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa
    34501 tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc
    34561 aataaatgca cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt
    34621 tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc
    34681 caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg
    34741 ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata
    34801 tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata
    34861 aggacttctg caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc
    34921 cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa
    34981 ttcttccggc ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt
    35041 tttcacagac cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt
    35101 tggttctagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat
    35161 attaataaaa ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg
    35221 cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg
    35281 cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat
    35341 tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt
    35401 ccccttgaaa atactccggc agcgttagcc accggagagg aaattatttc agcgatccct
    35461 taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct
    35521 cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc
    35581 ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac
    35641 gtaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa
    35701 cttctggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa
    35761 gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca
    35821 ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga
    35881 cgttttccaa caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc
    35941 gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc
    36001 gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga
    36061 agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta
    36121 catctccggc attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt
    36181 tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa
    36241 ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa
    36301 gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg
    36361 gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca
    36421 gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca
    36481 gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct
    36541 tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg
    36601 caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta
    36661 aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac
    36721 tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg
    36781 tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact
    36841 accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc
    36901 cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac
    36961 ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca
    37021 ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca
    37081 gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga
    37141 ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca
    37201 tgatccgccg aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc
    37261 ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa
    37321 aaccgagagt gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg
    37381 cctccgtcac catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca
    37441 agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc gaaagccgat
    37501 tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc
    37561 tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca
    37621 acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc
    37681 cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa
    37741 gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt
    37801 cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa
    37861 cagtttttca tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt
    37921 gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt
    37981 caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc
    38041 ccagtgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc
    38101 tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc
    38161 ggcagcgtac agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag
    38221 cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg
    38281 gttttcgaca aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat
    38341 atcgaaacca accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc
    38401 ggtaccgatg cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc
    38461 cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt
    38521 tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca
    38581 aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgtatttca
    38641 atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca
    38701 gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct
    38761 tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt
    38821 cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta
    38881 ctgatgagcc agcaaaagcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg
    38941 cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca
    39001 aatcctggtt ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt
    39061 aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg
    39121 ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt
    39181 cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt
    39241 cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca acccaggtac
    39301 atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat
    39361 cacctttagc atctttcgac gagaacagcg cataggtggt atcggttccg atacgtaccg
    39421 tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag
    39481 cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat
    39541 tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta
    39601 acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc
    39661 aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt
    39721 tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag
    39781 cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc
    39841 tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata
    39901 atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt
    39961 gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg
    40021 gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt
    40081 aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc
    40141 gcatcgagcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg
    40201 gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg
    40261 cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca
    40321 atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg
    40381 gcgggagttg caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt
    40441 atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg
    40501 taacgtatcg aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt
    40561 gaacaccgag cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag
    40621 atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac
    40681 ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg
    40741 gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc
    40801 catctcgata atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac
    40861 gtttttatcg cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg
    40921 cagctgctgg cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg
    40981 gcggtacggt ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg
    41041 gatcgggatc accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag
    41101 tttcgtgccc atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg
    41161 gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt cagcacattg
    41221 acgggtaaac aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg
    41281 caggaaatca aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt
    41341 acggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg
    41401 gaaagcaacc ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa
    41461 gcggtttgtg gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa
    41521 gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat
    41581 gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc
    41641 gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc
    41701 gctggagctg ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg
    41761 ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa
    41821 gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac
    41881 cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc
    41941 gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa
    42001 acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat
    42061 cagctgtgat ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa
    42121 gagaatggcg gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg
    42181 atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc
    42241 accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc
    42301 gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa
    42361 agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgtgga tcaggctaac
    42421 cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc
    42481 acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg
    42541 cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat
    42601 cgacttttta tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta
    42661 tccgacctac ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc
    42721 aagactgtat taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt
    42781 caactcaccc agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg
    42841 ggtaatttta ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg
    42901 cagcttaccg acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac
    42961 ataggcttta cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg
    43021 cgcaggtggt gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc
    43081 gaccggtgca ggttccggtt caaactcggt
//
LOCUS       DADLCQ010000019        40981 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000019 DADLCQ010000000
VERSION     DADLCQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.019"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            44..175
                     /locus_tag="JQG95_000937"
     ncRNA           44..175
                     /ncRNA_class="other"
                     /locus_tag="JQG95_000937"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(359..1036)
                     /gene="narI"
                     /locus_tag="JQG95_000938"
     CDS             complement(359..1036)
                     /gene="narI"
                     /locus_tag="JQG95_000938"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY5887583.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1036..1746)
                     /gene="narJ"
                     /locus_tag="JQG95_000939"
     CDS             complement(1036..1746)
                     /gene="narJ"
                     /locus_tag="JQG95_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY5887584.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1743..3281)
                     /gene="narH"
                     /locus_tag="JQG95_000940"
     CDS             complement(1743..3281)
                     /gene="narH"
                     /locus_tag="JQG95_000940"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY5887585.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3278..7021)
                     /locus_tag="JQG95_000941"
     CDS             complement(3278..7021)
                     /locus_tag="JQG95_000941"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY5887586.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWDTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7414..8805)
                     /gene="narK"
                     /locus_tag="JQG95_000942"
     CDS             complement(7414..8805)
                     /gene="narK"
                     /locus_tag="JQG95_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY5887587.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9144..10940
                     /gene="narX"
                     /locus_tag="JQG95_000943"
     CDS             9144..10940
                     /gene="narX"
                     /locus_tag="JQG95_000943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY5887588.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10933..11583
                     /gene="narL"
                     /locus_tag="JQG95_000944"
     CDS             10933..11583
                     /gene="narL"
                     /locus_tag="JQG95_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY5887589.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11584..12978)
                     /gene="ychO"
                     /locus_tag="JQG95_000945"
     CDS             complement(11584..12978)
                     /gene="ychO"
                     /locus_tag="JQG95_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY5887590.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13163..13516
                     /gene="ychN"
                     /locus_tag="JQG95_000946"
     CDS             13163..13516
                     /gene="ychN"
                     /locus_tag="JQG95_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY5887591.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13560..14255)
                     /locus_tag="JQG95_000947"
     CDS             complement(13560..14255)
                     /locus_tag="JQG95_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY5887592.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14413..14643)
                     /gene="chaB"
                     /locus_tag="JQG95_000948"
     CDS             complement(14413..14643)
                     /gene="chaB"
                     /locus_tag="JQG95_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY5887593.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14913..16013
                     /gene="chaA"
                     /locus_tag="JQG95_000949"
     CDS             14913..16013
                     /gene="chaA"
                     /locus_tag="JQG95_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY5887594.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16418..16525
                     /locus_tag="JQG95_000950"
     CDS             16418..16525
                     /locus_tag="JQG95_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY5887595.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(16674..17528)
                     /gene="kdsA"
                     /locus_tag="JQG95_000951"
     CDS             complement(16674..17528)
                     /gene="kdsA"
                     /locus_tag="JQG95_000951"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY5887596.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(17564..18373)
                     /locus_tag="JQG95_000952"
     CDS             complement(17564..18373)
                     /locus_tag="JQG95_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY5887597.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(18377..18769)
                     /locus_tag="JQG95_000953"
     CDS             complement(18377..18769)
                     /locus_tag="JQG95_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY5887598.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(18766..19599)
                     /gene="prmC"
                     /locus_tag="JQG95_000954"
     CDS             complement(18766..19599)
                     /gene="prmC"
                     /locus_tag="JQG95_000954"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY5887599.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(19599..20681)
                     /gene="prfA"
                     /locus_tag="JQG95_000955"
     CDS             complement(19599..20681)
                     /gene="prfA"
                     /locus_tag="JQG95_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY5887600.1"
                     /translation="MKPSIVAKLEALHERHEEVQTLLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(20723..21979)
                     /gene="hemA"
                     /locus_tag="JQG95_000956"
     CDS             complement(20723..21979)
                     /gene="hemA"
                     /locus_tag="JQG95_000956"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY5887601.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            22193..22816
                     /gene="lolB"
                     /locus_tag="JQG95_000957"
     CDS             22193..22816
                     /gene="lolB"
                     /locus_tag="JQG95_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY5887602.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22816..23667
                     /gene="ispE"
                     /locus_tag="JQG95_000958"
     CDS             22816..23667
                     /gene="ispE"
                     /locus_tag="JQG95_000958"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY5887603.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            23818..24765
                     /locus_tag="JQG95_000959"
     CDS             23818..24765
                     /locus_tag="JQG95_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY5887604.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            24890..26569
                     /gene="dauA"
                     /locus_tag="JQG95_000960"
     CDS             24890..26569
                     /gene="dauA"
                     /locus_tag="JQG95_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY5887605.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(26624..26902)
                     /gene="ychH"
                     /locus_tag="JQG95_000961"
     CDS             complement(26624..26902)
                     /gene="ychH"
                     /locus_tag="JQG95_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY5887606.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            27180..27764
                     /gene="pth"
                     /locus_tag="JQG95_000962"
     CDS             27180..27764
                     /gene="pth"
                     /locus_tag="JQG95_000962"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY5887607.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            27881..28972
                     /gene="ychF"
                     /locus_tag="JQG95_000963"
     CDS             27881..28972
                     /gene="ychF"
                     /locus_tag="JQG95_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY5887608.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            29815..32682
                     /locus_tag="JQG95_000964"
     CDS             29815..32682
                     /locus_tag="JQG95_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5887609.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(32782..34710)
                     /gene="dhaR"
                     /locus_tag="JQG95_000965"
     CDS             complement(32782..34710)
                     /gene="dhaR"
                     /locus_tag="JQG95_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY5887610.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTVDLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            34929..35999
                     /gene="dhaK"
                     /locus_tag="JQG95_000966"
     CDS             34929..35999
                     /gene="dhaK"
                     /locus_tag="JQG95_000966"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY5887611.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLEACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            36010..36642
                     /gene="dhaL"
                     /locus_tag="JQG95_000967"
     CDS             36010..36642
                     /gene="dhaL"
                     /locus_tag="JQG95_000967"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY5887612.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            36653..38071
                     /gene="dhaM"
                     /locus_tag="JQG95_000968"
     CDS             36653..38071
                     /gene="dhaM"
                     /locus_tag="JQG95_000968"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY5887613.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            38391..40088
                     /locus_tag="JQG95_000969"
     CDS             38391..40088
                     /locus_tag="JQG95_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY5887614.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(40167..40606)
                     /locus_tag="JQG95_000970"
                     /pseudo
     CDS             complement(40167..40606)
                     /locus_tag="JQG95_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        10135 a        10629 c        10223 g         9994 t
ORIGIN      
        1 ctttcaaaag ccccggaatt ctcaaacgaa tctgcaatca aatattctgc ccaagcgggg
       61 aaggatgaga agcttcgacc aaggttcgac ttgagcgcca gcgagagagc gttgccgcag
      121 gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac caccatcgct
      181 tccttgaata atcaaaacct gttactccga gctaattttg cccgatggca ccacgtttac
      241 cgggcctaca atatcctccc ctgtaggtcg gataagcgaa gcgcatccga caactcaccc
      301 cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta aaattcgttt
      361 agtgacgagc gcgcaccagc tggtacttac gtgtcagata ctccaccggt acgctccaga
      421 tgtgtaccag acgcgagaac gggaacagca ggaataacgt catccccagc accaggtgca
      481 gacggaagat aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca
      541 ccgactgcgc ccagccaacc agtttcatca tctcgctacc gtccatatgc tgagcggaga
      601 acggaatggt cagcaggccc agcgcgcact ggataacgag cagcgacagg atcaggatat
      661 ccgctccggt agtggttgca cgcacgcgtg ggctgaacat acgacgtttc agcaaaagca
      721 cgccgccaat cagacacagc acgccgctgg caccaccagc aaacatcgcc attttctgtt
      781 tcacttcaat cggcagccag gcttcataca tccagtgcgg cgtcaacata ccgaagaagt
      841 gaccgacaaa aatccccaga ataccgatat ggaacaggtt cgacgccagg ttcatccctt
      901 tgcgatccag catctggctg gacgccgcgc gccaggtgta ctgcccgtag tcataacgca
      961 gccagctacc aatcaggaag accgccccgg ctatgtacgg gtagatatca aagaagaaca
     1021 tattcaggaa ttgcattagt gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac
     1081 ggcaccggca aagcgacgct gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc
     1141 aaagaattta acctgctctt cttcccagac agcatccagc gcctgcggcg tatcatcgcg
     1201 cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg atagcggtat tggccaattt
     1261 cagcagcaga tcaaacatca cggcataacg gctttcacgc tgttgcagac gcgcgctcag
     1321 caatgccaga atcggcgcga tatctttcaa accttccacg gcttcgcttt gcggcagctg
     1381 cgacaggtac tccagataca gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa
     1441 gccgtgctgc tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt cacgggattc
     1501 accgtgaaca tgttcaaata gcaacagtga cgtggcgcgg ccacggtcga acagttcgct
     1561 gtactggacc tgggcatcga gcggatccat cgccgttaaa tcgcgcagga aaatgcccag
     1621 cgcatgggca tcttctttcg acagattttt cgacgcagca atcgcctcaa acatctcttg
     1681 ttgatgctgc cataaggcag catccggata ttcaaggaga cgcgatacaa tcacgagttc
     1741 gatcatggat gcggctccgt tttgctggtc acatcgatgg catcgatacg acggctgttg
     1801 aacagattga atttggtatc tgagccgtgg cagccatcac caaaggtaaa gccgcagcca
     1861 tttttctccg ggaaggcttc ccgtgccagt tcacgatgac tactcggcac cacaaagcga
     1921 tcttcgtagt tagcaatcgc cagataacgg tacatctcct gtgcctgggc ttcggtcaga
     1981 ccgacctctt ccagcgcacg ggtatcaact ttaccgtcaa cagtttcagc acgtttgtaa
     2041 tgacgcatcg ccagcatacg tttcagcgca cgcagtaccg gtttggtatc accggcggtc
     2101 agcagattcg ccagatactg taccggaata cgcaagcttt cgacgtctgg cagaattccg
     2161 ttgctgccca gctcgcccgc gtctgctgca gactgaatcg gagacagagg cggcacgtac
     2221 cagaccatcg gcagtgtgcg atattctgga tgcagcggca gcgctagttt ccattccatc
     2281 gccattttat aaaccggcga ctgctgtgca gcttcaataa cgctcagcgg aataccgtct
     2341 ttaatcgcct gctcgatgac tttcggatcg ttcggatcga ggaacacgtc cagctgacgc
     2401 tggtagagat ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac
     2461 agcagcacgc caagataacg gatacgaccg acacaggttt ctgagcacac ggtcggctga
     2521 cccgcttcaa tacgcggata gcagaagatg cacttctcag acttaccgct cttccagttg
     2581 aagtagattt ttttgtacgg gcatccggtg atgcacatac gccagccacg gcatttatcc
     2641 tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc
     2701 gccacacatg ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg
     2761 ttttcgaact ggctatacat cgccttctgg atgttgtcga agttcttgtc tttcgccagt
     2821 ttatcaaact caccacccag gtcatcttcc cagttcggcc ctttttcgat tttcgccatc
     2881 cgttccccgg taatcagcga acgcggacgg gcaatcggct gcgatttgct gccttccggc
     2941 gcggtatgca ggttctgata atcaaaatcg aacggctcgt aataatcgtc gatccccggc
     3001 agatgcgggt tagcaaagat tttacccagc agcatggctc ggttacccat gcgcggttgc
     3061 agtttgccat tgattttacg gatccagccg cctttgtatt tttcctggtt ttcccagtca
     3121 gtcgggaagc cctggcccgg cttggtttcc acgttgttga accacgcgta ttccacgcct
     3181 tcacggctgg tccagacgtt tttacaggta actgaacagg tgtggcagcc gatgcactta
     3241 tcgagattca gcaccatgcc gacttgtgaa cgaattttca ttttacgctc tcctgtacct
     3301 ggtcattgcc ttcgccatct aaccagtcaa tgttcttcat cttacgcact acaacaaact
     3361 catcgcggtt agaacctacg gtgccgtagt agttaaagcc gtatgccaga tgggcatagc
     3421 cgccgatcat atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg ataccaccac
     3481 gctgttgggt aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg tggtacatca
     3541 tggtcatccc tgccggaaca cgctggctga caaccgcacg ggcagtcagt gcaccgttgc
     3601 tgttgaagac ttcaatccag tcgttatcgg cgatacccag atctttggca tcggcttcac
     3661 tcaaccagac caccggacca ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg
     3721 tggagtggat accccacttc tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc
     3781 cgttggattt ctggcctatc acttctttca ccgaacgggt gtcgatcggc ggacgataaa
     3841 ccagcaggct ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt tgctgacgac
     3901 cagagagcgt acgccatggg atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa
     3961 cgtgttcatc ttccagacca gaccaagtcg ggctggagat aattttgcgc ggctgtgcct
     4021 gaatatcgcg gaagcggatc ttctcgtctt ctttattcag cgccagatgc gtatggtcac
     4081 gaccggtaaa ttcgctcagg gcagcccagg ctttcacggc tacctgaccg ttggtttccg
     4141 gtgccagcgt caggatcatc tctgccgcat caattgcggt gttcagcatc ggctggcctt
     4201 ttgccggacc ttccgctttg gtgtagttga gcttacgcag cagatccatc tcgctctggg
     4261 tgtcccaagc aatcccttta ccgccgttac cgattttctc catcagcggg ccgatagagg
     4321 taaagcgttc gtaagttgcc ggataatcgc gctctacgac cataatgtgc ggtgcagttt
     4381 tgcctgggat caggtcgcat tcgccttttt tccagtcttt cacatccagc ggctgcgcca
     4441 gttcagcggc agagtcgtgc tggataggca gcgtgacgat gtcggtttct ttacccagat
     4501 agccgacgca cacttcggag aatttcttcg cgatggcttt gtagatttcc cagtcgcttt
     4561 tcgcttccca ggccggatcg accgccgcag acagcgggtg aataaacgga tgcatatccg
     4621 aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga
     4681 gacaggtgct cgacagacgg aagtccagcg taaccaccag atccagcttg ccttccagac
     4741 cattgtcctg ccagtccact tcttccggct tcacgccgcc ctgttgcccc agatctttac
     4801 cctggatacc gtgctccgtc cccagcaggt acttgagcat aaactcatga cctttaccgg
     4861 aagaaccgag caggttagaa cgccagatga acaggttgcg cgggtggttt ttaccgtttt
     4921 ctggttgttc tgccgcaaaa cggatggaac cctctttcag ggatttcacc gtatagtcca
     4981 ccggattcat cccggctttt tccgcttcgc cagcgatagt cagcgggtta gtgcctaact
     5041 gcggtgcaga cggcagccag cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc
     5101 cggtatagcg ggatttgtcc gccatcggtg acagcaactc ttccgccgtg acggtttcat
     5161 aacgccactg gctggagtgg ttatagaaat aagaagtgct gttcatgtga cgcgccggac
     5221 gctgccagtc aagggcaaac gccagcggct gccagccggt ttgcggacgc agtttttcct
     5281 gacctacata gtgcgcccag ccgcccccgc tctgaccgac acagccgcag aaaatcagca
     5341 tgttgatcag accacgatag ttcatatcga ggtgatacca gtggttcagc cccgcaccga
     5401 cgataatcat cgaacgaccg tgcgttttat cagcgttatc ggcaaattca cgggcgatgc
     5461 gaataatctg gctgcgagaa acgccggtaa tctgctcggc ccaggccggg gtataagctt
     5521 tcacatcgtc atagctggtt gcacagttaa cgtcgttcag gccacgttcc agaccgtagt
     5581 ttgccagcgt cagatcataa acggtggtca ccagggcggt gctgccatca gccagttgca
     5641 ggcgtttcac cggcagtttg tgcagcagca cgttttccag ttccactttg ttgaagtgtt
     5701 ccgtgccgtc gccaccaaag tacgggaagc ctacctcagc gatctcatcc tggctaccca
     5761 gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc
     5821 atttgccctt ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc atttcgccat
     5881 tggtattaaa ggcgacagtt ttccattccg gattgttttc ctggcccagc gcatcaacca
     5941 gatcagcagc gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct tccagcatca
     6001 ccagcatcgg catgtcggtg tagcgacgca catagtcggt gaaatactgg cttgggttgt
     6061 cgaggtggaa ttcacgcagc attacgtggc ccatcgccag cgccattgcc gcatcggtgc
     6121 cctgtttcgg tgccagccac agatcgcaca gtttggcgat ttcagcgtag tctggtgtga
     6181 cggcaacagt tttggtgcct ttgtaacgca cttcagtaaa gaagtgagca tccggggtac
     6241 gcgtctgcgg cacgtttgaa ccccaggcga tgatgtagct ggagttatac cagtcagcag
     6301 attccggtac gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt
     6361 accagtcgta gaagcttaag caagtaccgc caatcagcga gagatagcgt gcacccgatg
     6421 cgtaagaaac catcgacatt gccggaattg gcgagaaacc agcaacacgg tccgggccgt
     6481 agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc gttcacctcc tgccaagaag
     6541 aacgaacaaa tccaccgcgt ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt
     6601 caatgataga agcccatgcc tcaaccggat cgctatgcag cgccttcgct tcacgccaca
     6661 ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg gttggcactg taaagatacc
     6721 aggagtagct ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga tccggacggg
     6781 tacgcggata gtcagtctgc tgggtttccc aggtgaccag accgtttttg acgtagattt
     6841 tccagctgca ggagccggtg cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct
     6901 gccaacgctg gcgatatcca tcctcccagt cacggttggt attgagaagc tggccatgcc
     6961 catcggcaaa ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg aatttactca
     7021 tcggttttct cctgtgggag cctgtcggcc ctctgataaa tcgacattgc taatgtttaa
     7081 gggcaaggta acgctctgaa acagcgtggg aattgataac gatcaagagt gatggggatg
     7141 aaaaataaag taaattccta agatacccct ttaggataat taacagttac agcataacat
     7201 gctgtaaatt aagacgtttt atacctgtag ttatttaaac tattaagatt tttatgcaaa
     7261 tgggtattaa ggagtattcc ccatgggtaa catattaatg gctcttaatg aaatccggcg
     7321 aaataagcca tataacacta atatgttgta actaaatgac gcataaaaaa agcgcggcat
     7381 catgccgcgc aaaggataat caaatattaa gatttatttt ttagaatgcc gaccatatac
     7441 cgcccaagta atcactacgc aggcgatata gaaaatcaaa aatactttca ttgcacctac
     7501 tggcgaaccc gttaatgcca gcgagctacc aaacgctttc gggataaaga agccaccaat
     7561 cgcgccaatc gcagagatga aacccagcgc cgctgccgtg ccggttgccg cttcacgcat
     7621 cgcacgttcg tcagaacccc cttctgcttt cacgcgatcc attgtcagtt tacggaagat
     7681 cactgaaatc atctggaaag tggaaccact acccagccca gctgtcagga acagcgccag
     7741 gaagaccgcg aagaacgcca tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa
     7801 cagcaggccg ctgaaaatcg ccatcagaat aaagttcacc agcgtgacac gagttccgcc
     7861 cagacggtca gataatgcac cacctgcaga acgcgccagc gcaccaataa acggcccgaa
     7921 gaaagcgtat tgcagaatct gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc
     7981 cgcggagaag ccgatgaagg agccgaaggt tgccagatac agcaggctca taatccacag
     8041 atgaccccgt ttgagtaccg gcaactgctc cttgatggag gctttcgagg tagcaagatc
     8101 gttcatgcca aaccacgccg caatggtgaa gatggcaagg aacggcaccc atatccagga
     8161 cgcattcgcc agatacagct cagtcccatc cggctgtttg acgccctggc taccaaatac
     8221 tgcgaaaatc gacagtgata ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat
     8281 gtttcccaga ccaccattca gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa
     8341 gctgatgttt gccatactgg atgcgaagtt cgcgccagca aagccgcata gcagagagat
     8401 gatgatgaag acgctataag gcgtggaggt atcctgcacg gcaaaaccca gccagacgca
     8461 aggaataatc agaataccgg tgctgaacgc cgtccagcga cgaccaccga agataggaac
     8521 cataaaggag tatggaacac gtaataacgc gccagaaacc gaaggcagcg cagtcaacat
     8581 aaatagctga tcggtcgtaa aattaaaacc gactttcggt aagttcaccg caacagcgct
     8641 gaacaacatc catacgcaaa acgccagcag cagacaggga acggaaatcc acaggttgcg
     8701 gctggcaata cgttgaccgc gttgttgcca gaacgcagga tcttccggtc gccaatctgt
     8761 tatgacagct ccagtagccc tttcggaggc agatgagtga ctcatagaca cctctgatac
     8821 tcgtttcgat gtctgccacc ttagtgtctg gagctaaagg caatttgatg taaatcaaac
     8881 gataagataa ctttatctat cattgatatt tatcattacc catagtgagt acagtgattt
     8941 cataaaaatt atgagatttt tcacggtgct gtagaatccc tacccttacc gatgtaaagc
     9001 gactaaccac acggcaaata aggagtaact ctttccggct atgggtatac ttcagccaat
     9061 agccgagaat actgccattc cagaatatat cgtcacattc attaaggtta ttgctcattt
     9121 aaagcctgaa ggaaggggtt tacatgctta aacgttgtct ctctccgctc accctggtta
     9181 atcaggttgc gcttattgtg ttgctttcta ctgctattgg actggcaggg atggcggttt
     9241 ctggctggct ggtgcaaggc gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc
     9301 tgcgcatgca aagttaccgt ctgttggcgg cagtgccatt aagcgagaaa gacaagccct
     9361 taattaaaga gatggaacaa acggcattta gcgccgagtt gactcgagca gcagaacgag
     9421 acggacaact ggcgcaatta cagggtttac aagattactg gcgtaatgaa ctgatccctg
     9481 cgctgatgcg tgcacaaaac cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg
     9541 ggcttgatca gctggtatct ggttttgacc gcaccacgga aatgcgtatt gagacagtgg
     9601 tactggtcca tcgggtaatg gcggtattta tggcactttt actggtgttc actattatct
     9661 ggttgcgggc gcgactgcta caaccgtggc ggcaactgct ggcaatggcg agtgccgtca
     9721 gccatcgcga ttttacccaa cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg
     9781 gaactgcgtt gaacaatatg tctgcagaac tggccgaaag ttatgccgta cttgagcagc
     9841 gggttcagga gaaaaccgcc gggctggagc ataaaaatca gatcctctct tttttatggc
     9901 aggctaaccg ccgtttgcat tcccgcgccc cgctgtgtga acgcctgtca ccggtactca
     9961 acggcttaca gaatttaacc ctgctacgtg atatcgaact gcgggtgtat gacactgatg
    10021 atgaagagaa tcatcaggag tttacctgcc agccagatat gacttgtgat gataaaggct
    10081 gccagctctg cccgcgcggc gtattacccg ttggtgatcg cggcacgacc ctgaagtggc
    10141 ggctggctga ctctcatacg cagtacggta ttttgctggc gaccctgccg caggggcgtc
    10201 atcttagcca tgatcaacaa caactggtgg ataccctggt tgaacaactt accgccacgc
    10261 tggcgctgga tcgccatcag gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca
    10321 ccattgcgcg cgaactgcat gattctattg cccaatctct ctcttgcatg aagatgcagg
    10381 tgagttgttt acagatgcag ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc
    10441 agatccgtaa cgaactgaat gcatcctggg cgcagttgcg tgaattgctc accacattcc
    10501 gcttgcagct caccgagcct ggattacgtc cggcgctgga agcgagttgc gaagagtaca
    10561 gcgccaaatt tggcttcccg gtgaagctgg attatcaatt gccgcctcgt ctggtgcctt
    10621 cacatcaggc aatccacttg ttgcaaattg cccgtgaggc attaagtaac gccctcaaac
    10681 attcgcaagc gagtgaggtc gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga
    10741 ccgtccagga taacggctgc ggcgtgcctg aaaatgccat ccgcagcaat cactacggca
    10801 tgataataat gcgcgaccgt gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg
    10861 aatcaggtgg caccgaagtg gtggtcacct ttattcccga aaaaactttc acagacgtcc
    10921 aaggagatac ccatgagtaa tcaggaaccg gctactatcc tgctgattga cgatcacccg
    10981 atgctgcgaa ctggcgtaaa acagcttatc agtatggcac cagatatcac cgtggttggc
    11041 gaagcgagta atggcgaaca gggtattgaa ctggcggaat ctcttgatcc cgatctgatc
    11101 ctgttagatc tcaatatgcc cggcatgaac ggtctggaaa cgctggataa actgcgcgaa
    11161 aagtccctct cagggcgcat tgtggtattc agcgtctcta accatgaaga agatgtggtc
    11221 accgcactga aacgcggcgc ggatggctat ctgttaaaag atatggaacc ggaagatctg
    11281 ctgaaagcat tgcatcaggc agctgctggc gaaatggtat taagcgaagc attaacgcct
    11341 gttctggccg ccagcttgcg cgctaaccgt gccactactg agcgcgatgt taaccagtta
    11401 accccacgcg agcgcgatat tctcaagctg attgcccagg gtttgccgaa caagatgatt
    11461 gcccgccgcc tggatatcac cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag
    11521 aaaatgaagc tcaagtctcg cgtggaagca gcggtatggg tgcatcagga gcgcattttc
    11581 tgattacggt tcccagcgca gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt
    11641 tagcgtgatc tcattggagg agacacgctg cccctggtta tcttccacca ccacagacaa
    11701 tcgccagtga ttgcttgccc cttccccgtt ctgccagtca ggcatgatca gcgtccagcc
    11761 ctccgcgctg ttggcttgtg cgcccggcgt caaacttaat atctgcgtat cgccctgcca
    11821 aatcagttgc cgaataccgt aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc
    11881 aggttttaga tcccacggcg gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta
    11941 ctcaagagtc ggtagattat ttcgctgcgg attgtcatag cgactaccac gtaacgactg
    12001 actttcggca acctcgcccg cagaaagttg ttttttgagc ggtacaccaa agcggtaatt
    12061 aagattcagc ccgaggttat tttgattttc gccactttcc ccctgtttat gctgggccgt
    12121 cacagtgact aatggcacag gggtgtaatt taatcccaga ctcaacgcga cgggattgtg
    12181 ataacccgta ccagagttaa acaaatcaac acgatcacca aaatactgtt ctaggctgac
    12241 actggtattg aggtgttgat agaacggcat gcgcatccga gctgtcaggt cgtacccgcg
    12301 cgccatccgt tgttcctgcg tggctgtctg ttcatgccat gcagcaaacg gctgataaaa
    12361 gtttgccgat aatcgcaaat attcgcccca cgcttcggca ccaaagcccg ctcgctgaag
    12421 attttcgtcc tgcaagttgt cataaaaagt gttataaccc accagccagt tgccgcgcgc
    12481 ccagcgttgc ccaacgccca cattgctcac caacccatta tcctgctgag taagaccaag
    12541 ctggctccag gtgagataac gatcattatc ttgtaacggc acaaaccagc ttccacgact
    12601 gccggtgaaa tgtccttcgt tatccacttt gacgtcaaca ctggcatttc cccacggtga
    12661 tagccaggac tctacgtgct gattaacctg ttgactaagc gcgtcgcgga cttttcccaa
    12721 tgcgaaagct tttgcctgct cgccagtatc cagcccgtta tcattcatac tggtttcgcc
    12781 gaaatctttc acaatttcag caaagtgttt ttccccatca tgattttcgg gagccatgcc
    12841 taaatccggc agaccatcat tgttattatc aaagggattt gccgcttttt gctcgaaggt
    12901 agattgtgcg ttagctgtac cgcctgctac aagcaagagt aaataaaacg ggataatgcg
    12961 aggaacgaaa cggctcaaaa cgtcggtaac ttccggtaat aatgaaaaac catagcctta
    13021 aagaataacg attactggca acttttgtcg tattttctcg cgctttcagg aaaatcctta
    13081 aaaatgtgac cgattggtca gacgctgcag aagcagtaag atagaagatg agtgtctaat
    13141 tccctgaata caggagtaaa taatgcaaaa aatcgtgatc gttgccaatg gcgcacctta
    13201 cgggagcgaa tccttgttta acagcttgcg gctggccatt gcgttacgag agcaggagag
    13261 caatctggat ctgcgtctgt tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca
    13321 aaaaccaggg gaaggctaca acattcagca aatgctggag atccttaccg ctcagaatgt
    13381 accggtgaaa ttgtgcaaaa cctgtaccga cgggcgcggg attagcacac ttcctctgat
    13441 tgatggggtg gaaatcggta ctcttgtgga actggcgcaa tggacgctgt cagccgataa
    13501 agtgctcaca ttttaatgtt taagggggcg tctgacgccc cactatgagg gaagtttact
    13561 caggcgaatc ccggccttaa cacaccatcc ggataattct ccgccagtag tttttttacc
    13621 gataccagca aatcattcag cccatcgtcc tgcattccca gtttgataag ctcctgttcc
    13681 agtgaaaaca ggtattgtgc attggttccc agcggaccgc tcgccgccgc aatcaacggc
    13741 gcgatgacct gagcgcgagt gtcagattca tattctggat gtcgcgggtc cataataaac
    13801 acaatggcgt ttactgtgcg tccatcatca agatcaagct gacaccaggt tggcagataa
    13861 cagccggtaa tcatctcgcg cttccacaac agggttagtt cctgctccag cgtctcttct
    13921 ggtagtcgat aggcgacgcc tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc
    13981 ggctggtgcg cagtcccgcg cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca
    14041 accagtgtac cggtgcatga ttcggtaaac tccagtgccg gattccacat caacgatcca
    14101 taaccgaaga tccacaccgg tccctcatca ggtcgacaag ccagcgtcgc cgccagcgaa
    14161 gccgcccgtt gttctgctga ccataagagt gattcttcaa tggcaccaaa tgccgtttta
    14221 caatcggcat tcatcaagaa atcacgcgtt atcaccttac acctccgcca ctgcatactt
    14281 ccctgcgaca actttttggt gtcttccgga catttttacc gctcattttg aaaccactaa
    14341 cggaacaata tgcaatccac ggaccacttg caaggcaaca ccgcacgaat agctttaact
    14401 aagctaccgg ttttacgatt ttttatgcca tttatcatca tcccctttgg catattcatg
    14461 cttcacagca gcccaggcca ctttatgcgc ggtttcttcg cgactggcgt catcacgccg
    14521 atcttcttta tctttatatt gatcccatgc gctgttgaac gcttctttat agatatcctg
    14581 ggcatgagac ggtagaacgt gctttacgct ttccggcaga tcgcttttcg ttttatacgg
    14641 catcgttgac tccttttaca aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc
    14701 caaaacataa gaattttcat gtcagactgt tcttaagaaa cgctaaaaca aacattgtct
    14761 aaacgtaata cttattttta tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc
    14821 gtaaaatgtt accgatatcc agacactttc tgttattttt cctacctatt ttttacattt
    14881 tcgataaatt catctgcaaa gggataacca taatgtcaaa tgctcaagag gcggtaaaaa
    14941 cccgccacaa ggagacttcg cttattttcc cggttctggt gctggtagtg ctgttcctgt
    15001 ggggaagtag ccagacacta ccagtggtca ttgccatcaa tcttcttgcg cttattggta
    15061 ttttaagtag cgcctttagt gttgtccgtc atgcggacgt attagcccat cgcctgggag
    15121 aaccttacgg ttcgcttatt cttagccttt cagtggttat tcttgaagtc agtttgattt
    15181 cagctttaat ggcaaccggc gacgccgcgc caacgctaat gcgtgatacg ctctattcaa
    15241 tcattatgat tgttaccggt gggctggttg gcttttcatt attgttgggc ggtcgtaagt
    15301 ttgccaccca atatatgaat ctgtttggta tcaagcagta tttaattgcg ctgttccccc
    15361 tggcgataat cgtactggta tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc
    15421 aggcgttact ggtagcatta atttctgcgg caatgtatgg cgtatttttg ctgatccagg
    15481 ccaaaacgca tcaaagtttg tttgtctacg agcacgaaga tgacagtgat gacgacgacc
    15541 cgcaccacgg taaaacgtct gcccatagca gcctgtggca tgctatctgg ttgattatcc
    15601 atctgattgc cgttattgca gtcaccaaaa tgaacgccag ctcgctggag acattgctcg
    15661 acagcatgaa tgcccctgtt gcctttactg gcttcctggt ggcactgttg attctgtcgc
    15721 cggaaggttt aggtgcgtta aaagcagtgt tgaacaacca ggttcagcgc gcgatgaatc
    15781 tgttctttgg ttcagtgtta gcaacaattt cgcttaccgt acctgtcgtc acgctaattg
    15841 cctttatgac gggtaacgaa ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg
    15901 tggcctcttt agtgctgtgc catatctcct tctccaccgg acgtactaac gtgctcaatg
    15961 gcgcagcgca tctggcactg tttgccgcct atttgatgac gatatttgcc tgacagttat
    16021 atctcctccg atgacggaaa tcgggagaga taagataaat tcctgcttat tgtatttctt
    16081 catactacct ggacagctcg ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa
    16141 ccactgcaac agctgttgaa aagccggaat gtactcttta ttgcaaacat ttcagggatg
    16201 aagtgaagat gaattgagac gtagtatcca caccaagccg gaattatccg gtgaggcgca
    16261 atgttgcggg ggctttatcc ctggtggcat tggttgctgg aaagagaaaa cccccgcacg
    16321 ttgagaggta aaacctgaca acaaaacggg ggctaatctt gaaaccagac acttcaagaa
    16381 tagccgcaaa atgttgtcat tacaacaagg cggctatatg acgctcgcgc agtttgccat
    16441 gactttctgg cacgacctgg cagcaccgat cctggcgggg attattgccg cagcgattgt
    16501 cagctggtgg cgtaaccgga agtaaggtga aaacgggcgt caggctgccg tgatggcaat
    16561 atgcgcccga aaccggccgc aggggtaaca gcctgcggcc ttttttgtat ctggcaccca
    16621 tgaaaaaagt cttaacgcag aacgctaaga ctttattttt caagcaaaaa agattacttg
    16681 ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttcat ctgcttgagg
    16741 aacggttcca gtttagccag cggcagcgcg gatggaccat cacatttcgc atgttccgga
    16801 tccggatgcg cttcaataaa cagccccgcc agacctaccg ccataccggc tcgtgccagc
    16861 tcagccacct gagcacgacg accaccggaa gctgcgccaa acggatcgcg gcattgcagt
    16921 gcgtgggtca cgtcgaaaat caccggcgag ttaccagaca ctttcttcat aatgctgaag
    16981 cccagcatat cgacaaccag gttgtcatag ccgaagttag caccacgatc gcaaagaatc
    17041 actttttcgt tgccgccttc tttgaattta tcaacgatat tacccatctg tcccgggctg
    17101 acaaactgtg gtttcttgac gttaattacc gcaccggttt tcgccatggc ttcaaccagg
    17161 tcagtctggc gagcaagaaa cgccggcaac tgaatcacat ccacgacatc agcaacgggc
    17221 tgtgcctgac ttggttcgtg aacgtcggtg ataattttca cgccaaaagt ctgcttcaac
    17281 tcctggaaga ttttcatccc ttcttccagg cccggtccac gataagagtg gatggaggag
    17341 cggttggctt tgtcaaaaga ggctttgaac acgtaaggga tacccagttt ctgggtcaca
    17401 gttacgtagt gctcgcaaat gcgcatcgcc agatcgcgag attccagcac gttcataccg
    17461 ccaaacagta cgaacggcag gtcatttgct acgttgatgt cgccaatgct aaccactttt
    17521 tgtttcatag gatcgcctta atcttgagta aaatgtcgat taattaatgc agcacaatat
    17581 gtttatgcgc gatgttattt atttgcgcac ggatcatttc gctgatcggg tcttccggac
    17641 actgttcaac gaaataactt aaatcgttca acgcaacgtg ttcgcaatcc agttgcgcat
    17701 aaatcaaccc gcgatcgcga atttcatagg gatcttcagg gttgaattgt aataaagctt
    17761 cgctggtgcg taacgccagc tccatctgat tttcttccat caacgaggct ttgagtgtat
    17821 ccagcaattt gcggattacc tcaatgttat cagcttcatc aaggtcttca taaaacagtt
    17881 ccgccgacgg gctgatattt ccctttaacc atccgtccag catatgttcg cttaacgatt
    17941 caccgttaaa aggattaatc agccaaattt cgccatccgg acattcaatg cgcaatatca
    18001 gctgcgtagg gaaaatcacc ggcagcagcg gcaaatcgag acgattcgcg acccataata
    18061 aaaccgcacc taatgatacc gcactgccct gtcgattctt taacacctgg tccagccata
    18121 atgcatcgga aagacgataa acgccgcgtg aggctttaaa tccccagtcg ccgtaaaaca
    18181 gcgcgatcag tttttccagc tgctcttcta aaggcagaag ctggctgatt tcttccttcg
    18241 ccagactaac gagacgctcc agttcgtcgt aaacatcttg cgagggaaaa tcgcggcgga
    18301 ttgcttcgca agccaggatc atgccttcgc acaatggcgc tttattaaat tcgaaatcag
    18361 ctaacgatct catgacttac cccagtaacg gtacttttgt ggtggcgagt ttaatgatga
    18421 tgtacagcac caccaacgcc agcgggaagg caataatgcg cgcctgctga ctgtgcatac
    18481 gacgataatc gagtgcaata aaacccaaaa cgatataaat gataactcca aacagctttt
    18541 cagtcagcca ctgtgcctgc ccggaaaatg gcaggatgtg cgctttagcc atcaacgcaa
    18601 tgccgcttaa caataacacc gtatcgacaa ccggcggcac aatgcgcgtc cagcgagcaa
    18661 atgcctgtgg atgcttctga taacgtagcc agaaacgtaa ggttaatagc ccaacagaaa
    18721 gcgcgatact aataagatga acactaagca gtgtagaaaa acttgtcatt gataatagcg
    18781 gccgagcgtt acgcgctcgt tatcaccata gtcacggcag gtttcgacgt catgataccc
    18841 cgcgaggata aatgcttgtc gcaccgcttc gccctgctgc cagccatgtt ccagaagcag
    18901 aaagccgccg gataccagcg cgttacgcga ctgttcgatg atatgcacga tgtctgccat
    18961 tccactgtct gccgcaacca gcgcagtgag cggctcaaag cggacatcgc cttgttgaag
    19021 atgtgggtcc tgctcgtcaa tatacggcgg attgctgaca atcatcgcaa actgctgccc
    19081 ggctagcgcg ctaaaccagt cgctttgcag aatgtggata tttttgatcg ccagatgctg
    19141 ggcattacgt tgtgccaggg agacagcatc aggcatacga tctacagcga taatttcgca
    19201 gtccgggcgc tcgctagcca gcgcaagcgc aatcgccccg gtacccgtcc cgagatcgag
    19261 aatacggcaa ggttgttcag gcaaccgcgc cagtgcctgc tccaccagac actccgtatc
    19321 cgggcgcgga attaaggtcg ctggcgaaac aaataacggc aacgaccaga attctcgcac
    19381 cccggttaaa tgagcaatgg gttcaccatc gcgacgacgt gtcagtagcg catcaagttg
    19441 ctgacattgt tcgtcagtca gctgcgtttc accaaaggcg agaataaaag tacgccctct
    19501 gccggtaaca tgctccagca ggatttcagc atcacgccgc gggctttcgc tcgcctgaag
    19561 ttggcttatt gcttcacgta accagtgttg atattccatt attcctgctc ggacaacgcc
    19621 gccagttggt cggcctgatg ttcctggata atcggttcaa tcagcatatc cagcttacct
    19681 tccatcactt catccaggcg gtagagcgtc aggttgatgc ggtgatcggt aacgcgcccc
    19741 tgcgggaaat tgtaagtacg gttacggtcg ctgcgatcgc cactccccag caggttacga
    19801 cgggtagacg cttcggcctg ttggcgtttt gccatttcag cagcgtggat gcgagcaccg
    19861 agcactgaaa gcgccttcgc tttgttttta tgttgtgaac gttcgtcctg acattcaaca
    19921 acaatcccgg tcggcaagtg agtaatacga attgccgaat cggtggtgtt aacgtgctga
    19981 ccacccgccc ctgacgagcg gaaagtatca atgcgtaaat ctgctgggtt gatgtccggc
    20041 agttctgcgt caggcagttc tggcattacc gcaacggtac aagcagaagt atgaatacga
    20101 ccctgcgatt ccgtagcagg aacacgttgc acgcgatgac cgccggattc aaatttcaga
    20161 cgaccataca caccatcacc gctaattttg gcgatgatct ctttataacc accatgttca
    20221 ccctcgctgg cgctcatgat ttctacccgc cagcggcggg cttcggcgta acggctgtac
    20281 atacggaaca gatcgcctgc aaacagcgct gcttcgtcgc cgccggttcc ggcacggact
    20341 tcgaggaagg cgttacgttc gtcatcagga tcttttggta gtaatagaac ctgtaattgc
    20401 tgttccagtt gctcgctttt ttctttagct tcgcgcagtt catcctgcgc catctcacgc
    20461 atttcaggat cgtcgagcat catctgtgcg gtttcgatat cttcctgaac ctgttgccag
    20521 tcggtaaaac agcgcgaaac atcacttaac tgcgcatatt cgcgtgataa tgcgcgaaaa
    20581 cgttcctggt cggcgatagt ttgcgcgtca cccagcaacg tctgaacttc ttcatggcgt
    20641 tcatgcaggg cttccagttt ggcaacgata gaaggcttca taggcgtaaa tgcaccctgt
    20701 aaaaaaagaa aatgatgtac tgctactcca gcccgaggct gtcgcgcaga atattcaggc
    20761 gttcgttatc cccgtcacgg gcggcctgtt gaagtgattt cgttggcgca tggatcaagc
    20821 ggttagtcag tttccatgcc agatcctgca taatggcttg cgcgtcgccg ccctgctcaa
    20881 gggccgctaa cgctttggcg gttaactcat cgcgaacgtg ctctgcctgg ctgcgatact
    20941 cgcgaatggt ttcgctggcg ctttgtgctc gcagccacgc cataaattcg ctggtttcct
    21001 gagcgacaat agtttccgcc tcaaccgctg cggctttacg ctgcgccagg ttgtgcgaaa
    21061 tgatgctttg cagatcgtcc acactataaa gataagcatt cgccagtttg ccaacttccg
    21121 gctcaacatc gcgcggaacg gcaatatcca ccaacagcat tggttggtta cgacgacttt
    21181 ttaatgcgcg ctccaccatg cctttcccga taatcggtaa cgggctggcg gtggaactga
    21241 tgatgatatc ggcttcgcgc agacgttcgt cgatctcact cagggcaatc acttccgcgc
    21301 caacttcatc tgccagaatt tgggcacgtt cgcgagtgcg gttggcgata atcatcttct
    21361 gtactttatg ttcgcgcaga tgacgcgcta ccagctcgat ggtttcgccc gcgcctacca
    21421 gcaacactgt gaccgtagag agcgattcaa agatctgccg cgccagcgta caagccgcaa
    21481 aagcgacaga cacagcactg gcaccgatat ctgtttcagt gcgaacgcgt ttcgctacag
    21541 agaaagattt ctggaacatg cgttccagtt cgctggcctt catatgacct ttttgcgaat
    21601 cgacaaacgc ttttttaacc tgaccgagga tctgcggctc cccaagaacc agtgaatcca
    21661 ggccgctggc aacacgcatt aaatggctaa ccgcgtcgtt atcctgatgc cagtagaggc
    21721 ttttacgcag atcttcttca ttaagattgt gataatcgca aagccagcgg attaacgcct
    21781 cttgcaggtt atcctgctct tcaacgctaa gataaagttc cgtgcggttg cacgtcgaca
    21841 gcaccacgcc gccctgcacc atcggctgcg caagcaggct gtcaagcgcc tgatcgagct
    21901 tatccggcga aaacgataca cgttctcgca gcgatacagg tgccgtttta tggttgatac
    21961 cgagtgctaa aagggtcatg tctgcgggaa ataataccaa cgttgatagg gttagtctgc
    22021 ttgcatcata caggatgcgt aagatcaata aaagagagcg cccccttttg gagtaattgc
    22081 cgaaagccgt tagattctgg caattaagac aacttgaaca tagacgatag cggacggtaa
    22141 cgctagcatt aagggttata actgcaacgt atctcaagga cttgtcatca ctatgcccct
    22201 gcccgatttt cgtcttatcc gcctgctacc gctggctgct cttgtgctca ctgcctgttc
    22261 cgttaccacg cccaaaggtc ctggcaaaag cccggattcg ccacaatggc gtcagcatca
    22321 gcaagacgtg cgcaatctta atcagtatca gactcgcggc gcgttcgctt atatttctga
    22381 ccaacaaaaa gtgtacgccc gctttttctg gcagcaaacc ggccaggatc gctaccgtct
    22441 gctgctcact aacccattgg gcagcacgga actggagctg aatgctcaac cgggtaacgt
    22501 gcagttagtc gacaataaag gtcagcgtta taccgccgat gacgccgaag agatgattgg
    22561 caaattgacc ggaatgccaa ttccgctcaa cagcttgcgc cagtggattt taggtttacc
    22621 gggtgatgca accgactaca aactggacga ccagtaccgc ctgagcgaaa ttacctacag
    22681 ccagaatggc aaaaactgga aggttgttta tggtggttat gacaccaaaa cgcaacctgc
    22741 gatgccagcc aatatggaac tcaccgacgg tggtcaacgc atcaagttaa aaatggataa
    22801 ctggatagtg aaataatgcg gacacagtgg ccctctccgg caaaacttaa tctgttttta
    22861 tacattaccg gtcagcgtgc ggatggttac cacacgctgc aaacgctgtt tcagtttctt
    22921 gattacggcg acaccatcag cattgagctt cgtgacgatg gggatattcg tctgttaacg
    22981 cccgttgaag gcgtggaaca tgaagataac ctgatcgttc gcgcagcgcg gttgttgatg
    23041 aaaactgcgg cagacagcgg gcgtcttccg acgggaagcg gtgcgaatat cagcattgac
    23101 aagcgtttgc cgatgggcgg cggtctcggc ggtggttcat ccaatgccgc gacggtcctg
    23161 gtggcattaa atcatctctg gcaatgcggg ctaagcatgg atgagctggc ggaaatgggg
    23221 ctgacgctgg gcgcagatgt tcctgtcttt gttcgggggc atgccgcgtt tgccgaaggc
    23281 gttggtgaaa tactaacgcc ggtggatccg ccagagaagt ggtatctggt ggcgcaccct
    23341 ggtgtaagta ttccgactcc ggtgattttt aaagatcctg aactcccgcg caatacgcca
    23401 aaaaggtcaa tagaaacgtt gctaaaatgt gaattcagca atgattgcga ggttatcgca
    23461 agaaaacgtt ttcgcgaggt tgatgcggtg ctttcctggc tgttagaata cgccccgtca
    23521 cgcctgactg ggacaggggc ctgtgtcttt gctgaatttg atacagagtc tgaagcccgc
    23581 caggtgctag agcaagcccc ggaatggctc aatggctttg tggcgaaagg cgttaatctt
    23641 tccccattgc acagagccat gctttaagcc gggcaagctg agtttcggtg acaacgtcac
    23701 cttgttccag acgttgcatc gcgctcttta atacaccgcc tggaaaggat catgcctggc
    23761 ccgcacagtt ttcggcagat tctttccacc aatggacgca tgcctgaggt tcttctcgtg
    23821 cctgatatga agctttttgc tggtaacgcc accccggtac tagcacaacg tattgccaac
    23881 cgcctgtaca cttcactcgg cgacgccgct gtaggtcgct ttagcgacgg cgaagtcagc
    23941 gtacaaatta acgaaaatgt acgcggtggt gatattttca tcatccagtc cacttgtgcc
    24001 cctactaacg acaacctgat ggaattagtc gttatggttg atgccctgcg tcgtgcttcc
    24061 gcaggtcgta tcaccgctgt tatcccctac tttggctatg cgcgccagga ccgtcgcgtc
    24121 cgttccgctc gtgtaccaat cactgcgaaa gtggttgcag acttcctctc cagcgtcggt
    24181 gttgaccgtg tgctgacagt ggatctgcac gctgaacaga ttcagggttt cttcgacgtt
    24241 ccggttgata acgtatttgg tagcccgatc ctgctggaag acatgctgca gctgaatctg
    24301 gataacccaa ttgtggtttc tccggacatc ggcggcgttg tgcgtgcccg cgctatcgct
    24361 aagctgctga acgataccga tatggcaatc atcgacaaac gtcgtccgcg tgcgaacgtt
    24421 tcccaggtga tgcatatcat cggtgacgtt gcaggtcgtg actgcgtact ggtcgatgat
    24481 atgatcgaca ctggcggtac gctgtgtaaa gctgctgaag cgctgaaaga acgtggtgct
    24541 aaacgtgtat ttgcgtacgc gactcacccg atcttctctg gcaacgcggc gaacaacctg
    24601 cgtaactctg taattgatga agtcgttgtc tgcgatacca ttccgctgag cgatgaaatc
    24661 aaatcactgc cgaacgtgcg tactctgacc ctgtcaggta tgctggccga agcgattcgt
    24721 cgtatcagca acgaagaatc gatctctgcc atgttcgaac actaatcgaa cccggctcaa
    24781 agacccgctg cggcgggttt ttttgtctgt aatatccatt tgtatgacct atgcctcctt
    24841 cacctgccat ttagttgaca gatgatgcgc tcacggatga aacattattg tgaacaaaat
    24901 attttcctca catgtgatgc ctttccgcgc tctgatcgac gcttgctgga aagaaaaata
    24961 tactgccgca cggtttaccc gtgacctgat tgccgggata accgtcggga ttattgctat
    25021 cccgctggcg atggcgttgg ctattggtag tggtgtggcc ccccagtacg gtttatatac
    25081 cgcagctgtt gcggggattg tcattgctct aacgggtggg tcacgcttta gcgtttccgg
    25141 tccgactgcg gcatttgtgg taattctcta tcccgtttcg caacagtttg gactggcagg
    25201 actgctggtt gcgaccttgt tgtcgggaat ctttttgatt ctgatggggc tggcacgctt
    25261 tggtcgtctg attgagtata ttccggtttc cgtcacctta ggtttcacct cgggtatcgg
    25321 gatcaccatc ggtaccatgc agattaaaga ttttctcggt ctgcaaatgg cccatgtccc
    25381 ggaacattat ctacaaaaag tcggcgcatt atttatggcg ctgccgacca ttaatgtggg
    25441 tgatgctgcc attggcattg tgacgcttgg cattctggtt ttctggccgc gtctgggcat
    25501 tcgtttaccc ggtcaccttc cggccttgct ggctggttgc gcggtgatgg ggattgttaa
    25561 cctgctcggc ggacatgttg ctaccatcgg ttcgcaattc cactacgtcc tggccgatgg
    25621 ttctcagggt aacggtattc cgcaactgct gccgcaactg gtgctgccgt gggatctgcc
    25681 taattcagaa ttcacgctaa cctgggattc tattcgcacc ctgctgcctg cggcattctc
    25741 aatggcaatg ctcggggcga tcgaatctct gctctgcgct gtggtactgg atggtatgac
    25801 cgggactaag cacaaggcga atagtgaact ggtcggtcag gggctgggga atatcatcgc
    25861 tccattcttt ggtggtatta ccgctactgc tgccatcgcg cgttctgccg ctaacgtccg
    25921 tgccggggca acttccccta tctcggcggt gatccactct attctggtta ttctcgccct
    25981 gctggtactg gcaccactgc tctcctggct gccgctttcc gccatggcag ccctgctgtt
    26041 gatggtggcg tggaacatga gtgaagcgca caaagtggtc gacttgctgc gtcatgcgcc
    26101 gaaagatgac atcatcgtca tgctgctgtg tatgtcactg accgtgctgt ttgatatggt
    26161 tattgccatc agcgtgggga tcgtgctggc atcgctgctg tttatgcgtc gtatcgcacg
    26221 tatgactcgc ctggcaccgg tagtcgtaga tgttccagac gatgtcctgg ttctgcgcgt
    26281 tattggcccg ctgttttttg ctgctgctga aggcttattc acggacctgg agtcacgtct
    26341 tgaaggcaaa cggattgtga ttctgaagtg ggatgccgtt ccggtacttg atgctggtgg
    26401 tcttgatgcg ttccagcgtt ttgtgaagcg tctgcccgaa ggatgtgaac tgcgcgtgtg
    26461 caacgtggaa ttccagccac tgcgaactat ggctcgcgca ggcattcaac cgatcccggg
    26521 acgcctcgcg ttcttcccga atcgtcgcgc ggcgatggcg gatttataag tacgagattg
    26581 accagtcagc gcaaactgac tggtcagcaa actgcattat ttgttagcta tgacggcgat
    26641 tatcgctacg gcggcaacgt ttgtcatagt gacgaaccca ccagtaacgg tcgcacacct
    26701 gttcatgccc gccaacacgg gcacccgcca gccagagaat ggcaccgacg aaaatactta
    26761 gcactgcacc atgtgcgaaa tactggggca tattaaactg tggtaactgg ttgaggattg
    26821 aataccccac gccgaccacc attaccacca gacccaaccc catgagcacg ttaccgagta
    26881 tcgaagcgtt tttgcgtttc atatgtcacc tccggaactt tctgggttgt aacagggaat
    26941 acccctcttc cttatgtgta aagtatagac aacaccctgt gggttatgtg cgggcgtaat
    27001 cacaattaca acccttattt caacaaaact ttacaaataa acgcctgaac accttgactt
    27061 tctaatagtc cactcaggca attattacgt tatctgcatt tgatacgcag ttttttgtca
    27121 agcggggccg caaccagtaa actacgcgcc agttatgtac acactcagga caaaaaaacg
    27181 tgacgattaa attgattgtc ggcctggcga accccggtgc tgaatacgcc gcaacgcgac
    27241 ataatgctgg tgcctggttc gttgacttac tggcagagcg tttgcgcgct ccgctgcgcg
    27301 aagaggctaa attctttggt tatacttcgc gagtcaccct tggaggcgaa gatgtccgcc
    27361 tgttagtccc gactacattt atgaatctca gcggcaaagc cgttgcggcg atggccagtt
    27421 ttttccgcat taatccggac gaaattctgg tggcccacga cgaactggat ctgcctcctg
    27481 gcgtcgccaa atttaagttg ggcggtggcc atggtggtca caatggactg aaagacatca
    27541 tcagtaaatt gggtaataac cctaactttc accgtttacg catcggaatc ggtcatccgg
    27601 gcgataaaaa taaagttgtc ggttttgtgt taggcaaacc gcctgttagt gaacagaagt
    27661 taattgatga agccatcgac gaagcggcgc gttgtactga aatgtggttt acagatggct
    27721 tgaccaaagc aacgaaccga ttgcacgcct ttaaagcgca ataagtcgtt gtctgcggca
    27781 tttttgccga gtgccgtgta taataggcaa agttatttcc atttctgcaa tctgttagca
    27841 ataacaggtt gattattaag atattaaggt gatttaaatc atgggattca aatgcggtat
    27901 cgtcggtttg cccaacgtcg ggaaatctac cctgttcaac gcgctgacca aagccggtat
    27961 tgaagcggcc aactttccat tctgcaccat tgagccgaac acaggcgtcg taccaatgcc
    28021 tgaccctcgc ctggatcaac tggctgaaat cgtaaaaccg caacgtacgc ttcccacgac
    28081 catggaattt gtcgatatcg ccggtctggt aaaaggcgca tcgaaaggcg aaggtctggg
    28141 taaccagttc ctgaccaaca tccgtgaaac cgaagcgatc ggtcacgttg ttcgctgctt
    28201 tgaaaatgac aacatcattc acgtttccgg caaagttaac ccggctgacg atattgaagt
    28261 tatcaacacc gaactggcgc tggcagacct cgacacctgc gaacgtgcga ttcatcgcgt
    28321 acagaagaaa gccaaaggtg gcgataaaga cgcgaaagct gagctggcgg tcctggaaaa
    28381 atgcctgccc cagctggaaa acgcaggtat gctgcgcgcg ctggatttaa gcgctgaaga
    28441 gaaagcggct attcgttacc tgagcttcct gacgctgaaa ccaacaatgt acatagccaa
    28501 cgtcaacgaa gatggttttg aaaacaaccc atatcttgac caggtgcgtg aaatcgcggc
    28561 gaaagaaggt tctgttgtgg ttccggtttg tgctgctgtt gaagcagaca ttgccgaact
    28621 ggacgacgaa gaacgtgacg agtttatgca ggagcttggg ctggaagaac cgggcctgaa
    28681 ccgtgtgatc cgtgccggtt ataagctgct gaacctgcaa acttacttca ccgctggggt
    28741 gaaagaagtg cgtgcatgga ccattccggt tggagcaacc gcgccgcagg cagcgggcaa
    28801 aatccatact gattttgaaa aaggctttat ccgtgcacaa accatctcgt ttgaagattt
    28861 catcacttac aaaggtgaac aaggcgcgaa agaagcaggc aaaatgcgtg cagaaggtaa
    28921 agattacatc gtgaaagatg gcgatgtgat gaacttcctt ttcaacgtct aattaattta
    28981 ttgtctcatg aggtttcgct aaatctcatg aagagcgcaa aagtatataa aatccacgca
    29041 attgcgtgga tttttcattt caccgagtcc taatcattct tgtaacatcc tacacttttg
    29101 tgcttattgc ctacagtgaa ccacgagaat aattaaagac ataagtgttc agatgactga
    29161 tatctcatta gcagtgatat aacgtcagtt tttgttccac tacacatcag ccctcttcac
    29221 agctgatcaa tattgatctc gctccaatat gctggcgaga aaaggactta cctgaagggt
    29281 aatatcccgc agataaagtc cccgaataac gtgattacgt gcgatcttgg caagatgaaa
    29341 atgataacca gtagcacaat aataacgata ggccagaaaa tccattgaac aaaaatgaat
    29401 atcgatgttt tttgcttttt cttattagta atgcaaaaat ggcgcatatt gtttttgtta
    29461 tgcagataca tcaagcgaaa atcccataac taattaggat tattacaaaa aatcttcacc
    29521 aaatttttta accgctgttt gttgtaatat ttttgccata ttcatagtaa cgaattggaa
    29581 taagtacgac aacttaggga ataactcgat ataaactcac ctttgtaagg ggggggttaa
    29641 atatatatta attcctgtgt tcctgatttt gcccggtaat agagaattat tctcagagaa
    29701 gctcatcatg attgcaccat acagatatca tgatgataag aggatattat tcattgactt
    29761 aaacaaaatg acaaaatcca tagacccata gcctgggtac tatataggag tggtatgggc
    29821 atcaaacaac acaatgggaa taccaaagcc gatcgtctcg ctgaattaaa aatccgttcg
    29881 ccctcaattc aactgataaa atttggcgct attggtttga atgcaattat cttttccccc
    29941 ctgctgatag ctgctgatac aggaagtcaa tatggcacca atattactat taatgatggt
    30001 gacagaatta caggagatac cgccgatcca tcaggaaacc tctatggtgt aatgacccca
    30061 gcaggaaaca cgcctggcaa tatcaacctg ggtaatgatg tcaccgtcaa tgtcaacgac
    30121 gcctctggat atgcaaaagg aatcattatt cagggcaaaa acagctccct gacagctaac
    30181 cgactcacag tagatgttgt tggtcaaacc tctgccatcg gcattaactt aattggtgac
    30241 tatacccatg ctgacttagg cacaggcagc accattaaga gtaacgatga cggcatcatt
    30301 attgggcata gctcaacact aacagccact caattcacca ttgaaaactc gaacggtata
    30361 ggcctaacca tcaatgacta tggcaccagt gtcgatcttg gaagcggaag taaaatcaag
    30421 accgatggaa gtacaggtgt ttatatcggt ggtctcaacg gcaataacgc caatggtgct
    30481 gcgcgtttta cggcgacaga cctgacaatc gatgttcagg gctacagcgc catggggata
    30541 aacgtacaga aaaactctgt tgtcgatctc ggaacaaaca gtaccattaa aaccaatggc
    30601 gataatgctc acggcctctg gagctttggc caggttagcg cgaatgcact cactgttgat
    30661 gtaactggag ccgcggccaa tggcgtcgaa gttcgtggtg gtacaaccac tatcggtgca
    30721 gatagccata tttcttccgc gcagggcggt ggcctcgtca ccagtagttc agacgcgaca
    30781 atcaattttt ctggcacggc agcgcaacga aacagcatct tttccggcgg ttcttatggt
    30841 gcctcggccc agacggcaac ggctgttatc aacatgcaaa ataccgatat tacggttgat
    30901 cgtaatggca gtctggcgct gggtttgtgg gcgctcagcg gcggtagaat aaccggagac
    30961 agtttggcta tcaccggcgc ggcaggagcc agagggattt atgccatgac caacagccag
    31021 atcgacctca cgagcgatct ggtcattgat atgagtacac ccgaccagat ggccatcgca
    31081 acgcaacatg acgatggtta tgccgccagc cgcatcaacg cctcgggtcg tatgcttatc
    31141 aacggtagcg ttctttccaa aggtgggcta atcaatctgg atatgcaccc tgggtcggtt
    31201 tggacaggtt cctccctcag cgataatgtc aatggcggga aactggacgt tgcaatgaat
    31261 aacagcgtct ggaacgtaac aagtaattct aatctcgaca cgctggcgct gagccattca
    31321 actgtcgatt ttgccagcca cgggtcaact gccggcacat ttaccacatt aaacgtagag
    31381 aacctgagcg gtaacagtac ctttattatg cgtgctgatg ttgttggcga gggtaatggc
    31441 gttaataata gaggggattt attgaatatc agcgggagta gtgctggtaa tcacgtattg
    31501 gctatccgca accagggcag cgaggccaca acgggaaatg aagttctgac agtggtaaaa
    31561 accactgacg gcgcggcctc gttcagcgcg tcttctcagg ttgagttggg gggatatctg
    31621 tacgatgtgc gtaaaaatgg cactaactgg gagctttacg cttccgggac agttccggaa
    31681 ccgactccta atcctgaacc cacaccagct cccgctcagc ctcccatagt caaccccgat
    31741 cctacgcctg aacccgctcc cacgcctaaa cccaccacga ccgcagatgc tggcggcaat
    31801 tatctcaatg tcggttactt attgaactat gttgaaaacc gtacgctgat gcaacggatg
    31861 ggtgacttgc gaaatcagag taaagacggt aatatctggt tgcgcagtta tgggggaagc
    31921 ttggactcct ttgccagtgg caaactgagc ggctttgaca tgggttacag cggtatccag
    31981 tttggtgggg ataaacgtct ctctgatgta atgccgttgt atgtcggtct gtatattgac
    32041 tcaacacatg catcgccgga ctatagcgga ggcgacggta ccgcacgttc agactacatg
    32101 ggaatgtacg ccagttacat ggcacaaaac ggtttttaca gcgatctcgt tataaaagca
    32161 tcgcgccaga aaaatagttt ccacgtactg gacagtcaga acaacggcgt taacgccaac
    32221 ggcactgcga atggaatgag catctccctg gaagccgggc agaggttcaa cctgtcccct
    32281 actggttatg ggttctatat agagccgcaa acccagctta catacagcca ccagaatgag
    32341 atggctatga aggcgagtaa tggcctcaat atacatctga atcactacga atcgctgctg
    32401 gggcgtgcca gcatgatact ggggtatgac atcaccgcag gcaacagcca gctgaatgtc
    32461 tatgtgaaga ctggcgctat ccgcgagttt tcaggggata ccgaatatct gttgaacgac
    32521 tcccgggaga agtacagttt caaaggtaat ggctggaata acggcgtggg agtcagtgca
    32581 cagtataaca aacagcacac attctatctc gaagcggatt acacgcaggg taacctcttt
    32641 gatcagaagc aagtcaacgg aggatatcgc ttcagctttt aagtgcggga tagacaagcc
    32701 cttaaacgat gctgaaaatt gttagcgtgc gccggaatga aatacagatc actctgatgt
    32761 atcagaaatc gtttctgtct ttcatacccg gcgcttaaac tgccctgcat caatgccatg
    32821 ttgcttcatt ttccgccaca gcgtagtgcg gccgatccca agtaaagccg acatttcctg
    32881 aatgcgaccg cctgtgacct gggctgcgtt aataattgcc tctttttcaa cttccgcaaa
    32941 tgacagactg gtggaaaggc gggtggcgct gacatcatct gtcgcctgct cggtaaacag
    33001 atgttcaggt aaatcactga cgcgaatgcg cccgttatca ctactcagag caagattctc
    33061 gatgacgctg taaagttcaa aatcgttgcc tggccatgca caagaaacta ggcgagcgag
    33121 ggcatcgtca tcaattttca gccgcgtaga gaagcgtttt tcaagactgc gtaatttgtt
    33181 attcaccagc gccggaatgc tgccacgccg catacgcaga ggcgggatgg taatttcaaa
    33241 tgcatgcagc gcgtaataca gctggcgact aaaacgattt tgttccacca gcattgcgag
    33301 gtccacggtc gttgttgcaa tcactttgac atcaattggt attaaacgcc gcgcatccag
    33361 tcgcgtgata accccctgct tgataacctg cagcaaagca gactgtaact ccaccgccag
    33421 atattcaatc ttttcaagaa acagcgtgcc gccgtgtgcc agttccagcc gactcagacg
    33481 gccattttca ttgtccgtgc gatcgccacc aataaattct tccgccagcg cagcatcacc
    33541 atataactca caattgacgg cgatataagg acctgcagca cgctcgcttt cattatgaat
    33601 tgcctgactt agcagtgcct tgcccacgcc ctcttctcca caaagcagga caggaaagct
    33661 actgcgcgcc gcctggcgac caaaatgaat caagcggcgg gtttgcggat cgtcctgtgg
    33721 catatgagcg aaggtatggc tgacttttcc taattgactg gtcattaact gccgcatctg
    33781 ttccacagga tggagcaaca aaataaagct ggttccctgc gtttctatta tcggtttaag
    33841 ggttatcacc gcatcaataa actggtgctg gctttcaaag gttgcttcta cgtgtttgag
    33901 cggatgtgcc tgttttattg cttgttgcaa tacggcgggt aacgttaaga gttcagtgat
    33961 tgcccgtccc tgacttgccg tcgcgtcaag gcgcaagacc cgcgccgcct gggcattaat
    34021 aaattgcaaa ttaccctgct cgtcccagct aatcacgcca tcatccatac tttctaacag
    34081 ggcattaagt tgatttaaat gacggttagt ttcagcgagc aaactgtccg tcagcagtaa
    34141 attaccgacc tcgcgggcga ttgccaacgt caacggcaaa tcagctgcgg tagtttgctc
    34201 aaccggacac gccagcgcta ttgttcccgt caatcggccc ttgctgtcaa acaacggcgt
    34261 tgcacaaaag gcccagttcc agagtgcctg tttgaaatgt tgatcggcca tcgttttcac
    34321 ggcctgacca gagatagccg ctaacgatag cgcacaagta ccaataattc cctcggcgca
    34381 atacgtgccg tcattgaatc ccagtgcact tagctgctgc aaggtttgcg gatcgccatt
    34441 acggctgaga atgcaggcgg tttcatcgag gataaacagc gcacactctc gcggtgccat
    34501 atattcccag gcatcttcca gcgcagcctg cccgagcgtc agcatcgctt tcttacgccg
    34561 ataaatagaa gcaaatgtca cgccctgggc ctgatgtggt acgttccatg tctcccgttt
    34621 catcagctta ttgcatcgct cccaggaggt tgcaattaag ggagatatgc cccgaccatc
    34681 gttgttaaaa gcgccactca tatcccgcat cccttatgac aaaaggtgtt ccggctcgca
    34741 attttaagta ttaatacacg taaaagtttc aatatggaac attcattaaa gcatatgttt
    34801 cactatgaaa cataatttgg gctaattttc ttttcccttg cctgatgcac aatggattca
    34861 tcggcagtca gtggtcgccg tgtcgttgaa catcatccat gccctaccgt aattgctgga
    34921 gcaaaataat gaaaaaattg atcaatgatg tgcaagacgt actggacgaa caactggcag
    34981 gactggcgaa agcgcatcca tcgctgacac tgcatcagga tccggtgtat gtcacccgag
    35041 ctgatgcccc tgttgcagga aaagtcgccc tgctgtcggg tggcggcagc ggacacgagc
    35101 cgatgcactg tggttatatc ggtcagggga tgctttcggg ggcctgtccg ggcgaaattt
    35161 tcacctcacc gacgcccgat aaaatctttg aatgctccat gcaaattgat ggcggcgaag
    35221 gtgtactgtt gattatcaaa aattacaccg gcgatattct taactttgaa acagcgaccg
    35281 agttactgca cgatagcggc gtaaaagtga ccactgtggt cattgatgac gacgttgcgg
    35341 taaaagacag tctttatacc gccgggcgac gcggcgttgc caacacagta ttaattgaaa
    35401 aactcgtagg cgcagcggcg gagcgtggcg actcactgga ggcctgtgcg gaactggggc
    35461 gtaagttgaa taatcaaggc cactcaatag gtatcgctct cggtgcctgt accgttcctg
    35521 ccgcgggcaa accttctttt acccttgcgg ataatgagat ggagtttggc gtcggcattc
    35581 atggtgagcc aggtattgac cgccgccctt tctcttccct tgatcaaacc gtcgatgaaa
    35641 tgttcgacac cctgctggaa catggctcat accatcgcac tttgcgtttc tgggattatc
    35701 aacaaggcag ctggcaggaa gaaccacaaa ccaaacaacc gctccagtct ggcgatcggg
    35761 tgattgcact ggttaacaat cttggcgcaa ctccgctttc tgagctgtac ggcgtctata
    35821 accgcctgac cacacgttgc cagcaagcgg gattgactat cgaacgtaat ttaattggcg
    35881 cgtactgcac ctcactggat atgaccggtt tctcaatcac cttactgaaa gttgatgacg
    35941 aaacgctggc tctctgggac gccccggtcc acaccccggc ccttaactgg ggtaaataag
    36001 gagaaagcaa tgtcactgag cagaactcaa attgttaact ggctcactcg ttgtggcgat
    36061 attttcaaca ccgagagcga gtatcttacc gggctggatc gcgagattgg cgacgctgac
    36121 cacgggctaa atatgaaccg aggctttagc aaagtggtgg aaaaactccc tgctatcgca
    36181 gataaagata tcggtttcat tctcaaaaat accggtatga cgctgctttc cagcgtcggt
    36241 ggtgccagtg gtccgctgtt cggtaccttc tttatccgcg ccgcacaggc gacccaggca
    36301 cggcaaagcc tgacactgga agagctttat cagatgttcc gcgatggcgc ggacggcgta
    36361 atcagtcgcg ggaaagccga acctggcgat aaaaccatgt gtgatgtgtg ggtgccggtg
    36421 gtggaatcgt tacgtcagtc cagcgagcaa aatctctctg ttccggtggc gctcgaagct
    36481 gccagtagca tcgccgaatc cgctgcacaa agtacgatta cgatgcaagc ccgcaaaggc
    36541 cgcgccagtt atctcggtga acgcagtatt ggtcaccagg atcccggcgc gacctcggtg
    36601 atgtttatga tgcaaatgtt ggcgttagcc gcaaaagagt aaggaattgg tgatggtaaa
    36661 cctggtcata gtttcacata gcagccgact gggagaaggt gtcggtgaat tagcccgtca
    36721 gatgttaatg agtgatagtt gtaaaatcgc cattgccgcg ggaattgacg atccacaaaa
    36781 tcccattggt accgatgccg tcaaagtgat ggaggccatc gaatctgttg ctgatgccga
    36841 ccatgtgctg gtcatgatgg atatgggtag cgcattattg agtgctgaaa ctgcgctgga
    36901 attgctggct cccgagatcg ccgcaaaagt acgtttgtgt gctgcgccgt tggtcgaagg
    36961 tacactggca gcaacggtca gcgcggcctc gggggcggat atcgacaaag ttatctttga
    37021 cgctatgcat gcgctggaag ccaaacgtga acaactgggt ttaccgtcct ccgacactga
    37081 aatctctgac acatgtcctc cgtacgatga agaagcccgt tctctggcgg tggtcataaa
    37141 aaaccgtaac ggcctgcatg tacgtccggc ctcccggctg gtttatacct tatcgacatt
    37201 taatgccgat atgttgctgg aaaaaaacgg caaatgcgtc acaccagaga gtattaacca
    37261 gattgcgtta ctacaagttc gctataacga tacgctgcgc ctgattgcga aagggccaga
    37321 agctgaagag gcactgatcg ctttccgtca cctggctgaa gataactttg gtgaaacgga
    37381 ggaagtcgct ccacctactc tgcgtcccgt tctgcctgtt tcgggtaaag ccttttatta
    37441 tcaaccagtt ttatgtacgg tacaggcaaa atcaaccctg accgcggaag aagaacaaga
    37501 tcgattacgc caggctattg acttcacgtt attagatctg atgacgttaa cagcgaaagc
    37561 agaagccagc gggcttgacg atattgccgc aatcttttct ggtcaccata cactgttagg
    37621 tgatccggaa ctgctggcgg cggcaagcga actccttcag catgaacatt gcacggcaga
    37681 atatgcctgg cagcaagttc ttaaagaact tagccagcaa taccagcaac tggatgatga
    37741 atatctacaa gctcgctata ttgatgtgga cgatcttctg catcgcaccc tggtccacct
    37801 gacccaaacg aaagaagaac tcccgcagtt taactcgcca actattctac tggcggagaa
    37861 catttatcct tccacagtac tgcaactgga tccggcggtt gtaaaaggta tctgccttag
    37921 cgccggaagt ccggtatccc acagcgccct aatcgcccgt gaactgggga ttggctggat
    37981 ttgccagcag ggtgagaaac tgtatgcgat acaaccagaa gaaacgctaa cgctggacgt
    38041 taaaacgcaa cgtttcaacc gtcagggtta agccaccgtt cggatggcat cgttctgatg
    38101 tcatccggca atttacccag cactgatgtt ttttaactac ctgttatatt ttttaccaat
    38161 ttatgatgat ttttcttcgc taatcgcgaa ccaaatgtcg ctggctggct aataaacaga
    38221 cgttatttga tcactctatt tcgccgttat ttatcccttt aattcccttt tctaaaatgc
    38281 ctgaccgttc gcagaatgag atttcgatca tgcagctagt gcgatcctga actaaggttt
    38341 tctgatactt gaataccgtt ttattcggtt tcgccaaagg agaatgattg atgaaatccc
    38401 ccgcaccttc tcgcccgcaa aaaatggcgt taattccagc ctgtatcttt ttgtgtttcg
    38461 ctgcgctatc ggtgcaggca gaagaaacac cggtaacacc acagccgcct gatattttat
    38521 tagggccgct gtttaatgat gtgcaaaacg ccaaactttt tccggaccaa aaaacctttg
    38581 ccgatgccgt gccgaacagc gatccgctga tgatccttgc tgattatcgg atgcagcaaa
    38641 accagagcgg atttgatctg cgccatttcg ttaacgtcaa tttcaccctg ccgaaagaag
    38701 gcgagaaata tgttccgcca gaggggcagt cactgcgcga acatattgac ggactttggc
    38761 cggtattaac gcgttctacc gaaaacaccg aaaaatggga ttctctgtta ccgctgccgg
    38821 aaccttatgt cgtgccgggc ggacgttttc gcgaggtata ttactgggac agttacttca
    38881 ccatgttagg acttgccgaa agtggtcact gggataaagt cgcggatatg gtggccaatt
    38941 ttgctcatga aataaacact tacggtcata ttcccaacgg caaccgcagt tactatttaa
    39001 gccgctcgca accgcccttc tttgccctga tggtagagtt actggcgcag catgaaggcg
    39061 atgccgcgtt gaagcaatac ctgccgcaaa tgcaaaaaga atatgcttac tggatggacg
    39121 gtgttgaaaa cctgcaagcc ggacaacagg aaaaacgcgt tgtaaaactt caggatggta
    39181 cccttctcaa ccgctactgg gacgatcgcg atacgccacg accagagtca tgggtggaag
    39241 atattgccac cgccaaaagc aatccgaatc gacctgccac tgaaatttac cgcgacctgc
    39301 gctctgccgc tgcgtctggc tgggatttca gctcgcgctg gatggacaac ccgcagcagt
    39361 taaatacctt acgcaccacc agcatcgtac cggtcgatct gaacagcctg atgtttaaaa
    39421 tggaaaaaat cctcgcccgc gccagcaaag ctgcaggaga taacgcgatg gcaaaccagt
    39481 acgaaacgct ggcgaatgcc cgtcaaaaag ggatcgaaaa atacctgtgg aacgatcaac
    39541 aaggctggta tgccgattac gacctgaaaa gtcataaagt gcgcaatcag ttaaccgcgg
    39601 ccgccctgtt cccgctgtac gtcaatgcgg cagcgaaaga tcgcgccaac aaaatggcga
    39661 cggcgacgaa aacacatctg ctgcaacccg gcggcctgaa caccacgtcg gtgaaaagtg
    39721 ggcaacaatg ggatgcgcca aatggctggg caccgttaca gtgggtcgcg acagaaggat
    39781 tacaaaacta cgggcaaaaa gaggtggcga tggacattag ctggcacttc ctgaccaatg
    39841 ttcagcacac ctatgaccgg gagaaaaagc tggtggaaaa atatgatgtc agcaccaccg
    39901 gaacgggggg cggcggtggc gaatatccat tacaggatgg ctttggctgg accaatggcg
    39961 tgacgctgaa aatgctggat ttgatctgcc cgaaagagca accgtgtgac aatgttccgg
    40021 cgacgcatcc gaccgttaag tcagcaacga cgcaaccctc aaccaaagag gcacaaccca
    40081 caccttaacc agcgcttact ccgacaacgt cattcggctt ctccggatgg cgttgtcggc
    40141 agataacacc atatctatgg ttttttctac acagacggtt tttcaagtcc tggcgggaag
    40201 tattgcgcaa atgtttgcca tacgtggttg ccgaagatag cgtcatttct acgggtaagt
    40261 gtttcacttt catcacccgg cgatttacgc cgacgttggc tatataaagc atcaattgct
    40321 accgaatatt gttacggtgt tcactcatca acgttttttt gctttctgga gttaaattaa
    40381 taatcacata atccagacac tccagagaat ttagcaacca aaaaaagaat tcagaccatt
    40441 cctcttcgcg taactgaagc attgccgaac gggtattttg gcttaatctg ccagaaggat
    40501 tgatcaattc taaatgttca ataagcgcag ccaaaaccgc ctcactatcc gccccatttt
    40561 taacgcctga ctggctactg tctaaaatat ttattttctg aatcacacca aaaacaacga
    40621 aacaataatt aaaaaaggat tctgttgttg ttaccgggaa ccgacttatt tcgctcagaa
    40681 agtcgttcaa ccagctccgc agtgataaga ttcgctgatg atacagtgta tcctcactgc
    40741 gtgctggagg tgccagtata atatgttgat caatacagta tttattgtgt tcagtcgttt
    40801 cctgatgcgt ttggttctct gcggattgtc gctggcgaaa taaatggctt gcggtaaatg
    40861 ccgttacaga gcagcgcaac gcgtcaattc cttcggagtg cccggctttt ggcgagagtt
    40921 ccggttttat gcgtccaggt agcggaaatt tcacccgaat gagaaatgca ttaataaaat
    40981 g
//
LOCUS       DADLCQ010000020        40634 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000020 DADLCQ010000000
VERSION     DADLCQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.020"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            76..417
                     /locus_tag="JQG95_000971"
     CDS             76..417
                     /locus_tag="JQG95_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY5887615.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(408..1334)
                     /locus_tag="JQG95_000972"
     CDS             complement(408..1334)
                     /locus_tag="JQG95_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5887616.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1424..2422
                     /locus_tag="JQG95_000973"
     CDS             1424..2422
                     /locus_tag="JQG95_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY5887617.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2419..2637)
                     /locus_tag="JQG95_000974"
     CDS             complement(2419..2637)
                     /locus_tag="JQG95_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887618.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="JQG95_000975"
     CDS             complement(2639..4654)
                     /gene="ligA"
                     /locus_tag="JQG95_000975"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY5887619.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4725..5711)
                     /gene="zipA"
                     /locus_tag="JQG95_000976"
     CDS             complement(4725..5711)
                     /gene="zipA"
                     /locus_tag="JQG95_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY5887620.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5941..6702
                     /gene="cysZ"
                     /locus_tag="JQG95_000977"
     CDS             5941..6702
                     /gene="cysZ"
                     /locus_tag="JQG95_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY5887621.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6887..7858
                     /gene="cysK"
                     /locus_tag="JQG95_000978"
     CDS             6887..7858
                     /gene="cysK"
                     /locus_tag="JQG95_000978"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY5887622.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8242..8499
                     /gene="ptsH"
                     /locus_tag="JQG95_000979"
     CDS             8242..8499
                     /gene="ptsH"
                     /locus_tag="JQG95_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY5887623.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8544..10271
                     /gene="ptsI"
                     /locus_tag="JQG95_000980"
     CDS             8544..10271
                     /gene="ptsI"
                     /locus_tag="JQG95_000980"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY5887624.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10312..10821
                     /gene="crr"
                     /locus_tag="JQG95_000981"
     CDS             10312..10821
                     /gene="crr"
                     /locus_tag="JQG95_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY5887625.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10863..11714)
                     /gene="pdxK"
                     /locus_tag="JQG95_000982"
                     /gene_synonym="thiJ"
     CDS             complement(10863..11714)
                     /gene="pdxK"
                     /locus_tag="JQG95_000982"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY5887626.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11819..12193
                     /locus_tag="JQG95_000983"
     CDS             11819..12193
                     /locus_tag="JQG95_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY5887627.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12226..12949
                     /locus_tag="JQG95_000984"
                     /pseudo
     CDS             12226..12949
                     /locus_tag="JQG95_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13153..14064)
                     /gene="cysM"
                     /locus_tag="JQG95_000985"
     CDS             complement(13153..14064)
                     /gene="cysM"
                     /locus_tag="JQG95_000985"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY5887628.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14198..15295)
                     /gene="cysA"
                     /locus_tag="JQG95_000986"
     CDS             complement(14198..15295)
                     /gene="cysA"
                     /locus_tag="JQG95_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY5887629.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15285..16160)
                     /gene="cysW"
                     /locus_tag="JQG95_000987"
     CDS             complement(15285..16160)
                     /gene="cysW"
                     /locus_tag="JQG95_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY5887630.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16160..16993)
                     /gene="cysT"
                     /locus_tag="JQG95_000988"
     CDS             complement(16160..16993)
                     /gene="cysT"
                     /locus_tag="JQG95_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY5887631.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16993..18009)
                     /locus_tag="JQG95_000989"
     CDS             complement(16993..18009)
                     /locus_tag="JQG95_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5887632.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18313..19104)
                     /gene="ucpA"
                     /locus_tag="JQG95_000990"
     CDS             complement(18313..19104)
                     /gene="ucpA"
                     /locus_tag="JQG95_000990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY5887633.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19291..20292)
                     /gene="murR"
                     /locus_tag="JQG95_000991"
     CDS             complement(19291..20292)
                     /gene="murR"
                     /locus_tag="JQG95_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY5887634.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20255..21151
                     /gene="murQ"
                     /locus_tag="JQG95_000992"
     CDS             20255..21151
                     /gene="murQ"
                     /locus_tag="JQG95_000992"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY5887635.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21155..22579
                     /gene="murP"
                     /locus_tag="JQG95_000993"
     CDS             21155..22579
                     /gene="murP"
                     /locus_tag="JQG95_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY5887636.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22759..23658)
                     /gene="yfeX"
                     /locus_tag="JQG95_000994"
     CDS             complement(22759..23658)
                     /gene="yfeX"
                     /locus_tag="JQG95_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY5887637.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(23754..24329)
                     /locus_tag="JQG95_000995"
     CDS             complement(23754..24329)
                     /locus_tag="JQG95_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY5887638.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24390..24839)
                     /locus_tag="JQG95_000996"
     CDS             complement(24390..24839)
                     /locus_tag="JQG95_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY5887639.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24826..25251)
                     /locus_tag="JQG95_000997"
     CDS             complement(24826..25251)
                     /locus_tag="JQG95_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY5887640.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25465..26334
                     /gene="amiA"
                     /locus_tag="JQG95_000998"
     CDS             25465..26334
                     /gene="amiA"
                     /locus_tag="JQG95_000998"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY5887641.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26338..27237
                     /gene="hemF"
                     /locus_tag="JQG95_000999"
     CDS             26338..27237
                     /gene="hemF"
                     /locus_tag="JQG95_000999"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY5887642.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27243..28295)
                     /gene="eutR"
                     /locus_tag="JQG95_001000"
                     /pseudo
     CDS             complement(27243..28295)
                     /gene="eutR"
                     /locus_tag="JQG95_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28341..28841)
                     /gene="eutK"
                     /locus_tag="JQG95_001001"
     CDS             complement(28341..28841)
                     /gene="eutK"
                     /locus_tag="JQG95_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY5887643.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(28854..29513)
                     /gene="eutL"
                     /locus_tag="JQG95_001002"
     CDS             complement(28854..29513)
                     /gene="eutL"
                     /locus_tag="JQG95_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY5887644.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30695..31486
                     /locus_tag="JQG95_001003"
     CDS             30695..31486
                     /locus_tag="JQG95_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887645.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32206..32601
                     /locus_tag="JQG95_001004"
     CDS             32206..32601
                     /locus_tag="JQG95_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY5887646.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32619..32882
                     /locus_tag="JQG95_001005"
     CDS             32619..32882
                     /locus_tag="JQG95_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887647.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            32905..33447
                     /locus_tag="JQG95_001006"
     CDS             32905..33447
                     /locus_tag="JQG95_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887648.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33544..33873
                     /locus_tag="JQG95_001007"
     CDS             33544..33873
                     /locus_tag="JQG95_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887649.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            33912..34118
                     /locus_tag="JQG95_001008"
     CDS             33912..34118
                     /locus_tag="JQG95_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887650.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34577..34915
                     /locus_tag="JQG95_001009"
     CDS             34577..34915
                     /locus_tag="JQG95_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5887651.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            34896..35174
                     /locus_tag="JQG95_001010"
     CDS             34896..35174
                     /locus_tag="JQG95_001010"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887652.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35167..36627
                     /locus_tag="JQG95_001011"
     CDS             35167..36627
                     /locus_tag="JQG95_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5887653.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36649..37470
                     /gene="murI"
                     /locus_tag="JQG95_001012"
     CDS             36649..37470
                     /gene="murI"
                     /locus_tag="JQG95_001012"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY5887654.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37589..38283)
                     /locus_tag="JQG95_001013"
     CDS             complement(join(37589..38037,38037..38283))
                     /locus_tag="JQG95_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY5887655.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(38802..40175)
                     /gene="eutA"
                     /locus_tag="JQG95_001014"
     CDS             complement(38802..40175)
                     /gene="eutA"
                     /locus_tag="JQG95_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY5887656.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40150..40629)
                     /gene="eutP"
                     /locus_tag="JQG95_001015"
     CDS             complement(40150..40629)
                     /gene="eutP"
                     /locus_tag="JQG95_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY5887657.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
BASE COUNT        10280 a        10458 c         9856 g        10040 t
ORIGIN      
        1 tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac ctcgtacagt
       61 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
      121 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
      181 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
      241 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
      301 gcgcagttgc agaaacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
      361 aatgtatccc ttctaaatcc tgttgaaaaa acgactcata tcgatatcta tatctgatac
      421 aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
      481 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
      541 tcaaccaccg gcgtaatgcg cttaaccacc agtgggctac cttcaggcaa tggtagcgcc
      601 agtgcaggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
      661 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
      721 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
      781 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
      841 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
      901 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcatgcagg
      961 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     1021 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     1081 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     1141 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     1201 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
     1261 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
     1321 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
     1381 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     1441 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     1501 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
     1561 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
     1621 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
     1681 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     1741 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     1801 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     1861 gctggttggt ctggtgatga atgttcacgg tgcaaggggc agccttgagc aggtcggtaa
     1921 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
     1981 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     2041 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
     2101 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
     2161 taacgtcttt atggcacgcc gactgagctt caataaggca gatgaaatta ctatcgtctt
     2221 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     2281 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     2341 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     2401 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
     2461 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     2521 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcaattaagc gacgcccttt
     2581 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     2641 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
     2701 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     2761 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     2821 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
     2881 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
     2941 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
     3001 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga
     3061 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
     3121 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgcca
     3181 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
     3241 ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
     3301 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
     3361 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
     3421 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     3481 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
     3541 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
     3601 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     3661 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
     3721 caaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
     3781 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
     3841 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
     3901 gcacccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgccac
     3961 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
     4021 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
     4081 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     4141 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
     4201 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
     4261 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
     4321 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
     4381 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
     4441 ggctgaaagc tgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
     4501 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     4561 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcaa agcgtcgttc
     4621 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     4681 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     4741 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     4801 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
     4861 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     4921 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     4981 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
     5041 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
     5101 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     5161 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     5221 aaagcctgtt gtgccggttg cggtgctgaa tgcacaggct gcggcgctgg cgcgacctgt
     5281 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacc
     5341 ggctgcgctg gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg ctgctggacc
     5401 ggttgacgcg gctgcgcaga cgcataaggc ggtaggtact ggtgttgcgg cgacggacga
     5461 gcagcctcat gctcctgagc gttagccggg gcatgattca cgcggtgaac acgaacctca
     5521 ccaacgccct catcatcttc gacatcctcg tcataagaat cgtcgtcacg ttttgacttc
     5581 attcgtttta atggccgatc gcggaacata gaagatcgtt ctttacggct ggtccagaaa
     5641 ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa tgattaatat cagacgcaaa
     5701 tcctgcatca ttatattctc tgttgttcta acaccttgcc accacggcaa acatttactc
     5761 actaagagta tttgccgatt acctcaagtg caagtgcact attaactttc acagcacaaa
     5821 gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt ccattgcgca
     5881 attacccggt aaaatacgca gaattttcct gggattggtc aaaaggagct catcctgatt
     5941 atggtttcat cattcacatc tgccccacgc agcggttttt actattttgc gcaaggctgg
     6001 aagctcgtct cgcaacctgg gattcggcgt ttcgttattt taccgctgct ggtcaatatt
     6061 ttgttgatgg ggggcgcatt ctggtggctc tttacacagc tcgatgtctg gatcccgact
     6121 ttcatgagtt acgttccgga ctggctacaa tggctgagtt atctgttgtg gcctctggct
     6181 gtcatctctg tgctgttagt gtttggctat ttcttctcca cgattgctaa ctggattgcc
     6241 gctccgttta acggtttatt ggctgaacaa ctggaagcac gattgactgg cgctacaccg
     6301 ccagataccg ggattttcgg tatcatgaaa gatgtgccgc gaatcatgaa acgcgaatgg
     6361 caaaaatttg tctggtatct gccgcgcgca attgtattac taattcttta cttcatccct
     6421 ggtattgggc aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg gatgttagcc
     6481 atccagtatt gcgattaccc cttcgataac cacaaagtgc cgtttaaaga gatgcgcacc
     6541 gccctgcgca cacgcaaaat caccaatatg cagtttggtg ctttaaccag cctgtttacg
     6601 atgatcccgc tgcttaatct gttcatcatg cccgttgccg tttgtggcgc gacggcgatg
     6661 tgggtcgatt gctatcgcga taaacacgcg atgtggcggt aacaatctac cggttatttt
     6721 gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc cttattccat cttgcatgtc
     6781 attatttccc ttctgtatat agatatgcta aatccttact tccgcatatt ctctgagcgg
     6841 gtatgctacc tgttgtatcc caatttcata cagttaagga caggccatga gtaagatttt
     6901 tgaagataac tcgctgacta tcggtcacac gccgctggtt cgcctgaatc gcatcggtaa
     6961 cggacgcatt ctggcgaagg tggaatctcg taaccccagc ttcagcgtta agtgccgtat
     7021 cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg ctgaaaccag gcgttgaact
     7081 ggttgaaccg accagcggta ataccgggat tgcactggcc tatgtagctg ccgctcgcgg
     7141 ttacaaactc accctgacca tgccagaaac catgagtatt gaacgccgca agctgctgaa
     7201 agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa ggcatgaaag gcgcaatcca
     7261 aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc aacaattcag
     7321 caatccggca aaccctgaaa ttcacgaaaa aaccaccggt ccggaaatct gggaagatac
     7381 cgacggtcag gttgatgtat ttattgctgg cgttgggact ggcggtacgc tgactggcgt
     7441 cagccgctac attaaaggca ccaaaggcaa gaccgatctt atctctgtcg ccgttgagcc
     7501 aaccgattct ccagttatcg cccaggtgct ggcaggtgaa gagattaaac ctggcccgca
     7561 taaaattcag ggtattggcg ctggttttat cccggctaac ctcgatctca agctggtcga
     7621 taaagtcatt ggcatcacca atgaagaagc catttctacc gcccgtcgtc taatggaaga
     7681 agaaggtatt cttgcaggta tctcttctgg agcagctgtt gccgcagcgt tgaaactaca
     7741 agaagatgaa agctttacca acaagaatat tgtggttatt ctaccatcat cgggtgagcg
     7801 ttatttaagc accgcattgt ttgccgatct cttcactgag aaagaattgc aacagtaatg
     7861 ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt ggcctgcttc
     7921 aaactttcgc ccctcctggc attgattcag cctgtcggaa ctggtattta accagactaa
     7981 ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc gattttatga
     8041 tttggttcaa ttcttccttt agcggcataa tgcttaatga cgtacgaaac gtcagcggtc
     8101 aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg tcgcgtctgt taaaaactgg
     8161 cgctaacaat acaggctaaa gtcgaaccgc caggctagac tttagttcca caacactaaa
     8221 cctataagtt ggggaaatac aatgttccag caagaagtta ccattaccgc tccgaacggt
     8281 ctgcacaccc gccctgctgc ccagtttgta aaagaagcta agggcttcac ttctgaaatt
     8341 actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact gcagactctg
     8401 ggcctgactc aaggtaccgt tgtgactatc tccgcagaag gcgaagacga gcagaaagcg
     8461 gttgaacatc tggttaaact gatggcggaa ctcgagtaat ttcccgggtt cttttaaaaa
     8521 tcagtcacaa gtaaggtagg gttatgattt caggcatttt agcatccccg ggtatcgctt
     8581 tcggtaaagc tctgcttctg aaagaagacg aaattgtcat tgaccggaaa aaaatttctg
     8641 ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag gcatcagccc
     8701 agctggaaac gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa gaagccatct
     8761 ttgaagggca tattatgctg ctcgaagatg aggagctgga gcaggaaatc atagccctga
     8821 ttaaagataa acacatgaca gctgacgcag ctgctcatga agttatcgaa ggtcaggctt
     8881 ctgccctgga agagctggat gatgaatacc tgaaagaacg tgcggctgac gtacgtgata
     8941 tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat tatcgacctg agcgccattc
     9001 aggatgaagt cattctggtt gccgctgacc tgacgccgtc cgaaaccgca cagctgaacc
     9061 tgaagaaggt gctgggtttc atcaccgacg cgggtggccg tacttcccac acctctatca
     9121 tggcgcgttc tctggaactg cctgctatcg tgggtaccgg tagcgtcacc tctcaggtga
     9181 aaaatgacga ctatctgatt ctggatgccg taaataatca ggtttacgtc aatccaacca
     9241 acgaagttat tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa aaagcagagc
     9301 ttgctaaact gaaagatctg ccagctatta cgctggacgg tcaccaggta gaagtatgcg
     9361 ctaacattgg tacggttcgt gacgttgaag gtgcagagcg taacggcgct gaaggcgttg
     9421 gtctgtatcg tactgagttc ctgttcatgg accgcgacgc gctgcccact gaagaagaac
     9481 agtttgctgc ttacaaagca gtggctgaag cgtgtggctc gcaggcggtt atcgttcgta
     9541 ccatggacat cggcggcgac aaagagctgc catacatgaa cttcccgaaa gaagagaacc
     9601 cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc ctgcgcgatc
     9661 agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg ttcccgatga
     9721 tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac aaacaggaac
     9781 tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg gtggaaacac
     9841 cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt agtatcggca
     9901 ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg atttcacacc
     9961 tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt gatgcttctc
    10021 atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa cgtgctacac
    10081 ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct atcccgcgca
    10141 ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca gagcaggctc
    10201 ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt gaagaaaaaa
    10261 caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat catgggtttg
    10321 ttcgataaac tgaaatctct ggtttccgac gacaaaaagg ataccggaac tattgagatc
    10381 attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt cgtttttgcg
    10441 gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg gtaacaaaat ggtcgcgcct
    10501 gtagacggta ccattggtaa aatctttgaa accaatcacg cgttctctat cgaatctgat
    10561 agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa aggcgaaggc
    10621 ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgatactgt cattgaattt
    10681 gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt tatctccaac
    10741 atggacgaaa tcaaagaact gatcaaactg tccggtagcg taaccgtggg tgaaaccccg
    10801 gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat cggcgccatt
    10861 ttttatgctt ccgccagcgg cggcaaaatc aattcatcgc tctcatgctg ctgggtgtag
    10921 cgcatcactt ccagtacgcg caaccccgca cggtgcaccg catcgtttaa cgccttccct
    10981 ttcagcaaac cactgataag ctgagcacaa aacaggtcgc cagtcccttt caggtcggtt
    11041 tttacccgtg aatgggaaat gacattcacg ctgtcggcac tgaccaccac cacctgcatc
    11101 tcctgatttt cttcattacc ggaggcgctg gtaatcacca cccattttaa tgtgtctgaa
    11161 agcagacttt ttgcggcagc aatggcactg tcgagatcgc ggcaattttt accggtcagg
    11221 atttccagct caaagatatt gggggtaatt ccctgcgcca gcggcagtaa atattgtcga
    11281 tacgcctcgg gaaggtcagg tttgacataa attccgctat caatatcgcc aatcaccgga
    11341 tcgaccatga tcaataggtc aggatggtct ttgcgtagcg cagtcagcca ctcggcaagg
    11401 attttgattt gcgatgccgt tcccatatag cccgtggtta cagcacgaag ttggcgcagc
    11461 gcatcacgct cctgaagcgc acgcaaatag ccgctaaacc attcgtccgg aatcgcacca
    11521 ccgtagaaag tgtcataatg cggcgtattg ctcagcaata ccgtcggcac ggcaaagaca
    11581 ttcaagccgt tctgttttat agcaggcacc gcaatgctgt tgcctacgct tccgtacact
    11641 acctgcgact gcacggcgac gatatccgcc tgcagcgccc tactcttatc gttaaacaac
    11701 aacaaactac tcatttaatt ttttctcctt gccgatgatc ctcatcgtat tccaaccgaa
    11761 actttacctg attctggcag tcaaatcggc tattacaaaa caaagataag gtaattcagt
    11821 gaagaaaatc atttgtctgg ccattacgct gctaatgaca ctccccgctt acgcgaagtt
    11881 aactgttcat gaagaagccc gcatcaacgc catgctggag ggattagcac agaaaaagga
    11941 tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat gaagcggttt cccacctgcg
    12001 tctgaagctc ggcaataccc gtaaccgcat tgacactgcc gagcagttta ttgataaggt
    12061 agcttcgtcg tcatcgatta ccgggaagcc gtatatcgtg aagatccccg gtaagagcga
    12121 tgagaacgca cagccttttt tacatgcgtt aattgcgcag acggataaaa cggtgcctgc
    12181 ggaaggaaat taatccgctt tggtaaggca tttacaggag gtaacatgaa aaaatgcttt
    12241 atttatcacg atgaaaaatc gaataaattt tggtggatag attacgaagg ggatagttta
    12301 gctgtcaact atggcaaggt aggtagtatt ggtaaattcc atacaaaaga attcgataat
    12361 gaagaacagt gtctgaaaga agccagtaaa ttgattgccg caaaaatgaa aaaaggatat
    12421 caagaaactc cagactttaa cttcatggat ctctactatt ttgatgatga agagattggg
    12481 ttacatctta aaacgtcaca cccaaacttc cagtgccatt ttactgatcc actttatatg
    12541 tgttgctggg atgaagaatc tccttttggt agcgatgaag gtgctgatgc tctaaacgaa
    12601 cttgaaaata gcctccgttc tggactgtgc tgatttccct caaatgttaa ttgaaactat
    12661 gtggggtatg aaatacatcg ctatggacag tattcttgaa gaggatgtgc gtgcgcaatt
    12721 actagccgat gaaatgagct ctatccagag caatatgatt acctacgcga ctgcattcgg
    12781 tcagatcaaa gtcatgggta aaatctccca taaacttaaa aagatgggac tcaatgcact
    12841 agcgcgtcat cagcttaccg caaaaattct tcaatggggt gacggtcagg actcaccaat
    12901 acttcaaaaa atgattgatg accttactgc ttttccacac gaaaattaaa aactgcattt
    12961 gtcggcagca acaactgccg acaaatcaga ttattgacaa agtgcacttt gtttatgccg
    13021 gatgcggcgt aaacgcctga tccggcataa aaatattgct aattcaatat attgcagaaa
    13081 cattgtaggc ctgataagcg gagcgcatca ggcagttttg cgtttgtcat cagtctccaa
    13141 tgctattaat ccttaaatcc ctgccccctg actaaaatgc tcttccccaa acactccggt
    13201 ggagaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac cagggttagc
    13261 ttttgccacc cgcagtgctc cagcaaccgc gccgcctgag ctgacgccac agaatattcc
    13321 ttcccgcacc gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa tatccagcac
    13381 ctcatccacc agcgcagcgt tgaaaatccc cggcagatat tccgcaggcc agcggcgaat
    13441 gcccggaata ctgctgcctt cttccggttg caggccaaca atggtcaccg gtttggattg
    13501 ttcgcgcata aagcatgaaa cgccggtgat agtgccggtc gtccccatgc tggagacaaa
    13561 atgagtgatg cgcccgccgg tttgctgcca gatttccggc ccggtggtgg tgtaatgcgc
    13621 ataagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac gattcgccat
    13681 ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa gaatcagttc
    13741 cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa
    13801 tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg tgttaccact
    13861 ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg cctcgacgat
    13921 catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt ccagttttaa
    13981 ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag gcgtattgcc
    14041 tattgtttgt tctaatgtac tcacaatctc tatccatacg tggtgttgcc tgatgcgacg
    14101 cttgcgcgtc ttatcaggcc tacaggttac aaaccttgcc attaaaaagc ccggatcgcg
    14161 ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt gcgagagcaa
    14221 gttcctcatc gcgggtttca atacgctcgt cgccgttata cagccgcgca tgttgcagac
    14281 caacgaataa acgctcgcca tgctgcgggg catcgtcgcc atgcatcacg accgtcagcg
    14341 gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct ttcgggctgg
    14401 cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg atatccactt
    14461 cccacgggcg caggaagaga tccaccggcc cctgatacgc aggtgtgtag cccagcggcc
    14521 agcgatgcgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg cggttcactt
    14581 cgcccataaa ttcgagtaca aaacgggtcg ctggttcgcg ccatacctga ttcggcgcat
    14641 cagcctgttc aatattcccc tgactcatca ccactacacg atcggctact tcggtcgcct
    14701 cttcctggtc gtgggtcaca aacacgctgg tgaatttcag ctcttcatgc agttgacgca
    14761 gccagcgacg cagctcttta cgcacctgcg catccagcgc gccgaacggt tcatcaagca
    14821 gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacacgctgt ttctggccgc
    14881 cggaaagctg cgccggataa cgatccgcca gatgcgcaag ctggaccatt tccagcaatt
    14941 ttgtcacttt cgctttgatg gctgcggcat tcgggcgctc gcgacgcggc agcaccgtca
    15001 ggccaaaagt gatattgtcg aataccgtca tatggcggaa cagcgcgtaa tgctggaaca
    15061 cgaaaccgac tttacgatcg cgcgcgtgca ggcgactcac gtcggtgccg tggaagcgaa
    15121 tatgcccgct ggtttgatgc tccagcccgg cgataatccg cagcagcgtg gttttcccgg
    15181 aaccggacgg ccccagcaac gcgaccatct gaccagaagg aatatccagt gagatatcgt
    15241 tcagcacctg ggtgcgacca aaagacttct tgatattggc aatctcaatg ctcatgatgc
    15301 tcctcctgct gcgcgcgttt ttcctgattc tccaggcgcc actgcaacat actctttaaa
    15361 aacagggtga taatcgccat caacgtcaac agcgcggcgg cggtaaagga gccgacggtg
    15421 ttgtagtcct gctccaacaa ttcaatctgt aacggcagcg aaagtgtttc gccgcgaatc
    15481 gagccggaaa ccaccgacac tgcaccaaac tcgccaatcg cgcgggcgtt ggtcagcacc
    15541 acgccgtaaa gcagcgccca gcgaatattc ggcaatgtga cgcgacggaa catctgccag
    15601 ccggacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct taacatcacc
    15661 ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag caccattccg
    15721 ggccaggaga acataatttg cagattatgt tcatccagcc agccgccgag cgggccgttg
    15781 gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacagc aaacggaatg
    15841 tccagtagcg tcagcagtaa ctggcgtccc gggaagttaa agcgcgtcac cagccaggcc
    15901 agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat caccgtcagc
    15961 cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat cagccccttg
    16021 ctgaatgcct gcacgaagat gtaaatcatc ggcaccagca ggatgaacgc cgaaaccagc
    16081 atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc
    16141 aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga ctttgcagag
    16201 tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca atcgcgctcg
    16261 ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc acttccgtct
    16321 tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga ctacgggtaa
    16381 acgacagcgc cacgcccgcc accagcgccg gagaaagctc cggcagcacc actttgcaga
    16441 aactctgcca gcgcgttgca ccaagcgttt ccgccgcttc ttcatattcc gggcctaact
    16501 cttccagcac cggctgcacg gtacgcacca caaacggaat gctggtaaag gccatcgcca
    16561 ccgcaatacc aagccaggta taggtgactt tgatatcaaa cttcgccagc cattcaccgt
    16621 aaaaaccgtt tacggaaaag agcgaggcca gcgttaaacc ggcgactgcc gttggcagcg
    16681 caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga taacgtgtta
    16741 aaatccacgc catcagcaga ccgaaaacgc cgttaaaaat cgatgccaca aacgccgaca
    16801 gcagcgtcac tttgtaggcc gcgaccacct gcgggttggt gatcacctcc cagtactgcg
    16861 cccagctcat ctcagaaagc tgcatcacca gcgcagagag cggcagcagc aatatcaggc
    16921 acacaaacag cagactggtg ccaaggctta aagtaaagcc cggcagcacg cgtcttgaag
    16981 aaactgcaaa cattacttac gccccgccgc taacagcttg tctaactcgc cgccgctggt
    17041 gaagtgggtt ttcatcactt ccggccatga gccaaatttg tcttccacgc ggaacagttc
    17101 ggtctgcggg aatttatctt tcagtttgtc catgacttcc gggttattca cacggtagta
    17161 atagtcggtg atgatggtct gcgcctgtgg gctgtagagc cagttcaggt aggcttttgc
    17221 ggctttttcc gtaccgttgg cctgcacatt tttatcgacc cacgccaccg ggaattccgc
    17281 cagaatattg gttttcggga tcaccacttc aaagccctgt gcttcatact gtttacggat
    17341 gttgttcact tccgattcaa agctaatcag cacatcgccc aagccacgct cggcaaaagt
    17401 ggtggttgcg ccacgaccgc cggtatcgaa cacttcaacg tttttcagga actgggtcat
    17461 aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc cccatgccgc
    17521 cagataggta taacgcgcgt tacccgacgt tttcgggttc gggaaaatca gcttcacgtc
    17581 ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac gcaccaggaa
    17641 gcccatggtg gagtagaacg gcgagctatt attcggcagg cgcgactgcc agtcagccgg
    17701 gatcagcttg cctttgtcgt gcaggatttg tacgtcggtc acctggttat aagtgacaac
    17761 gtctgctttt aagccctgca aaatcgccag cgcctgtttt gatgacccgg catgagattg
    17821 ttttatcgtc agtttgtcgc cgccgttatc ttttgcccat tgctgttcaa acggcggatt
    17881 cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt ccgttgcctg
    17941 tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt tcagtaagtt
    18001 aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca tatttataac
    18061 ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt aaaaagggaa
    18121 taagaacttg gttcaacagg cgtaattgtt gcaggcagtt tggacacgga cagcgcggag
    18181 aaaccggagc gtacacatag tacgtgagga tttcgagcac tgcccggggc caaaatgaca
    18241 aatgaaatag cctggtgaac ttagttcaag acgaaatcct ccccacacat gcagggagga
    18301 aacagaggtg aatcagatac cgacgctaac cgtctccggc agtgtgctgc cgccatcaat
    18361 cacattctgt gtaccggtta aatagctgga ttcatccgat gcgaggaagg ccgccagttc
    18421 gccgacttcc agcggatcgg cgaggcgaca cagcgggatt gcttttgcca tttcagtcag
    18481 caccgattct ggatcttccg ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg
    18541 gacgtatccc gggcagatgg cgttaacgcg aataccggat tgcgcgtact ccaccgccag
    18601 cgatttagtc aggccaacaa tcgccgcttt cgttaaggca tacgccgttt cgccaggatc
    18661 ggccaccata tcaccagtga ctgaagacat catcacaatg cgaccatctt tgcgcgcaat
    18721 catctccggc agcaccgcct tcgtgacgtt ccatacgcct ttaatattga tatcaatatg
    18781 gaaatcgcga tcttcatcgc tcatatcgag gaaactgccc agacgacaaa cgcctgcgtt
    18841 attcaccagg atatcaatgc gcccttcttt ttccttcgcg cgtttgatag ctgcggctac
    18901 cgacgccggg tcacgcacat cggcgacaac cgccgtacag cgatgaccac gaccacacag
    18961 ttcgtccgct agcttttcga tctcagggga gatatccagc aagattaggt tcgcgccatg
    19021 acgtgcaaaa gttctggcaa ttccttcgcc aattccctgc aatgcgcccg taatcagtgc
    19081 tgtcttgccc gtgagtttac ccattttaat gctctccttg ttaatacaga gcgttacact
    19141 ccattccctc tgaaaataca ggataaacaa aatattgctg cggatcagct cactaattca
    19201 ggaaaatctc aatccagtcg ctgctttatg catcatttca ggcgttgtgt tagctcactg
    19261 ctgcgctgaa tcattttcaa tgacgccaca tcattgatgt tgcacaagac caacaaacag
    19321 taaatcggta acagaatttt gcgccgtgcg ggtagacatc gacgagctac gccattcggt
    19381 ttcaccggaa acggtatcga gcgtgaaatg cgccagccgc cgcagcggag agtcagccag
    19441 agaagtaatg gcaatcaccg ttgccccctg ctttcgtgcc gcttctgcgc acaagacaat
    19501 ctctttttta ctgccgctgt aagagatagc gatttgtaca tcgccttttt tcaacgcctg
    19561 ggaaacggtg gcttgtacgt gcgtgtcggc ttcgcaggcc acgcgatagc caattttcat
    19621 cagtttgaac gacagatcgc gccccaccag cgccgatccg cccaggcccg ttatctggat
    19681 aaacggtgct ttactgatga cctcaatgat tttttgcagc cgcgcgtagt cgaacaacgc
    19741 acaggtttgc tccagcgcca gctctttttc gcgattcagt ttgcgggcaa tcacttccag
    19801 cgaatcatca ctggtaatcg aactgtgcag gtgcagcgcc gtggcattcg ttttctcccg
    19861 gctggcactg tattcgccaa ttaacgccat acgcaattca gtaaatcctt gcgcgccgag
    19921 cttctgggca aatttcacaa tgctcgactg gctgataccg agctgtttcg ccatctgccg
    19981 tgaagagacg gatttcagct cgctcactct ggcccgcaga aagtcagcaa ttttttgctc
    20041 gttcccggta aattcacttt ccgcattgcg tattttcgtc aggtacaaca tcttccactc
    20101 tcctgggctg gcagcaaaaa ttccgcttaa gttcttatga cgctcttcac actctgcgag
    20161 tgtagactca atgattcctt taagtctgtc aattcgggat atatgattcc atacatctgc
    20221 aattatttga tcgtaaatag taaggtcacc accgatgcaa ctagaaaaga tgattactga
    20281 aggctcgaac accgcctcgg ctgaaattga ccgcgtatcg acgctggaaa tgtgccggat
    20341 tatcaacgat gaagataaaa ccgtaccgcc tgccgttgat cgcgtcctgc cggatatcgc
    20401 cgcggcgatc gatgttatcc acgcccaggt cagcggcggc ggtcgtctga tttatctcgg
    20461 tgcgggaaca tccggtcgtc tggggattct ggatgccagc gaatgtccgc ccacctacgg
    20521 cgtgaaaccg ggtctggtgg ttggtttgat tgctggaggc gaatatgcca ttcagcacgc
    20581 ggtggaagga gctgaagata gccgggaagg tggtgttaac gatctgaaaa atattaattt
    20641 aacggcacag gatgtggtgg ttggcattgc tgccagcggt cgcacgccgt atgtgattgc
    20701 cggactggaa tacgcacgcc agctcggctg ccgcacagtg ggaatttcct gtaatccggg
    20761 gagcgccgtt tcaaccaccg ctgagtttgc cattacaccg attgtaggtg ccgaagttgt
    20821 taccggttct tcgcggatga aagcaggtac agcgcagaaa ctggtgctca atatgctttc
    20881 caccgggctg atgattaaat ccggcaaagt gttcggcaac ctgatggtcg atgtggtcgc
    20941 caccaacgaa aaactgcatg tgcgccaggt caatatcgtt aaaaacgcca ccggatgtaa
    21001 tgccgaacaa gcggaagcgg cgttaattgc ctgcgagcgc aactgtaaaa cggccattgt
    21061 gatggtgctg aaaaatctcg atgccgcaga agctaaaaaa cgcctggatc aacacggcgg
    21121 ctttattcgt caggttttag acaaggaata acccatggcc aaagagatca gcagtgaact
    21181 tctgaacacc attcttaccc gtgtcggcgg accgggaaat atcgccagtt gtggtaactg
    21241 tatgacgcgc ctgcgtctgg gtgtacatga cagttcactg gttgatccca atatcaaaac
    21301 gctggaaggc gtgaagggcg tcattttgac cagcgaccag gtgcaggtcg tttttgggcc
    21361 tggtaaagcg catcgtgccg caaaagcgat gagcgagctg ctgggcgatg caccggtaca
    21421 ggatgccgca gaaatcgccg cccagaacaa acgtcagtta aaagccaaac aaacctccgg
    21481 cgtgcaacaa tttctcgcca aattcgccac catcttcacg ccgctgatcc ccggttttat
    21541 tgccgccggt ctgctgctgg ggatagcgac gttaattgcc acggtgatgc acgttccggc
    21601 agatgctcag ggcacgctac ccgacgcgct gaattttatg aaggtgttca gcaaaggttt
    21661 gttcaccttc ctagtgattc tggtgggcta taacgccgcc caggcatttg gcggcacggg
    21721 cgtcaatggc gcaattattg ccgcactctt tttgctcggt tataaccccg ctgcaaccac
    21781 cggttactac gccggttttc acgatttctt tggtctgccc atcgatccgc gcggcaatat
    21841 tatcggcgtg ctgattgccg cgtgggcctg cgcccgcatt gaaggcatgg tgcgccgctt
    21901 tatgccggac gatctcgaca tgctgctgac ctcgttaatc accctgctga tcaccgctac
    21961 gcttgcgtac ctgatcatta tgccgctggg tggctggtta ttcgaaggta tgtcatggct
    22021 gtttatgcac ctgaacagta atccgttcgg ttgtgcggtt ttagccgggc tgttcctgat
    22081 cgccgtggta tttggcgtgc atcagggctt tattcctgtt tacctcgcgt taatggacag
    22141 ccagggattc aacagcttat ttcccatcct ttcaatggca ggcgcgggcc aggtgggcgc
    22201 ggcgctggca ctctactggc gggcgcaacc gcacagtgcg ctacgcagtc aggtacgcgg
    22261 ggcgattatt cccggcctgc tgggcgttgg tgaaccgctg atttacggtg tcaccctgcc
    22321 ccgcatgaag ccgtttgtta ccgcctgttt aggcggcgcg gcgggcggtt tgtttatcgg
    22381 attgatagcc tggtggggtc tgccgatggg cttaaacagc gcctttgggc cgtctggtct
    22441 ggtggcgctg ccgctgatga cttccgcaca aggcatcctt ccggcaatgg cggtttatgc
    22501 tggcgggatt ctggtggcat gggtttgcgg gtttattttc accacgctct ttggctgccg
    22561 taacgtcaat ctggactgat attatgaaac ggacaatgct ctatctttct ctgctggctg
    22621 ttagttgtag cgtcagcgcc gcaaaatacc ctgttctgac agaaggtagt tgatcaagta
    22681 tatgcggcgt taaagcagta ataaaaaggc cagtcagtaa tgttactgcc tggcctttaa
    22741 tcaatgaatc agaaacgctt acagcgccat caacttgtcc agcgacggtg cgaaataata
    22801 gccgccggtt accggtttgg tgaaacgcaa catcgcatca cgtttgccat cggtatcgcc
    22861 aaacatgctc agcagttgct gctcaatgtt atgcagacgc gcgcagtagg cgcagaagta
    22921 cagaccgtga gtgccactgg cggtgccgta cggcaggctc tggcgaacaa tcttcagccc
    22981 tttgccatct tctttcagat caacgcgggt gaggtgagag gtttccggac gttcgtcgcc
    23041 gtcaatctct tcgttggcct ctttggtgcg cccgatcatc atctcctgat cgtgaacgct
    23101 catccggttg agctgcttca ggttgtgctc ccaacgctgc acaaacacat agctgccgcc
    23161 ggaatccacg ccgtctttga taaccgccac ttcgcggcgc gtctcttcac ccgccgggtt
    23221 ttctgtaccg tcaacaaagc cgctcaggtc acgctcttcc acccaacgga agccgtggat
    23281 ctcttctttc acttcaatgc agtcaccaaa ggcttccatc gccgcctgag cgacagagaa
    23341 gtttacgtcg tgacgcagag aaagaatgtg gatcaacaca tcgaactggg tcgtcggcgc
    23401 aaggccttta ccgtagcccg gaaaatcttt cagctcttct gccccaacgc cgccgctcag
    23461 agcgcgccag gtgttgttac caaaggcaac caccgcacca agatgcgcgt ccgggaattt
    23521 cgcttcaaaa gttgccagtt tgtcggcaaa tgttttactg gccgcacgca gggcgtcaac
    23581 ttcccctttc acgttggctt cgatccaaat cgccgcgcgg caatgttctg gcaaaatgcc
    23641 actctgaacc tgagacattg ttcctcctga aaataagaat gccacgcaat ccgtggcatt
    23701 gttgagcgct attttacgtg ttttttcgcg acggcggttt gttcagacgc aaattaacgc
    23761 cgccagataa ttttactgac tttccagttt ttcagggtat cgtccgaagg cattaaccct
    23821 tcaggaccac gccattcccc gctgaactgg tagctaatat gttgactgcc ttcggcttta
    23881 cattcgacgg cacgattatc atcaccatca gctttttggc aattgccaaa tgctttgctg
    23941 taaaggtcgc taaacggtgt accgatttta acaccggtgt cagcaggaat atcgctatcc
    24001 agcacgtcaa tgcgactgat cgtgccttga tcgccgttaa tcaccatcgc cacgttgtcg
    24061 cctttcatca cttcaaaaaa gcgcaccacg ttgccgttcg cggttttcat tccgctgcgc
    24121 aggcgataat cgccatcaag cgcatcagca atggcttgtt cttgcagtgg tgtggacgcc
    24181 gttaattcac ccacgccctg ctcgctcact ttggtggacg atccaaacca gttccacggg
    24241 ttagcggcag accagttaac tgacgacatc gtggaacagc cggtcagcat caacggcata
    24301 gcgcataaca ttaaacgcag cgatttcatg tcacttcctt tggttattca ataacgttgc
    24361 ttggagtgca aattcaccaa aaagtgccgt tattcttttt cttcattaaa acaagcgcgt
    24421 aaacgtcgat tggtcagcag ccagattaac gccacaatat ccgccactac cagcgccaga
    24481 ccgataccgc taacggattc accgttcagc cacagccacg gttgccagca aagtaaaacc
    24541 acctgcgcca acagcaacag aaaatagagc acatgccagg tgcgaggaaa cgaagcccgc
    24601 cgaccgctca acaaaaacgc cagcaccgcc ggaataccgg gaatcagccc cagccagaaa
    24661 ttatcgtgat cgggataaaa cagatttagc agcgcagtac cctgctcacg cgacgcaccg
    24721 gcaatgacaa acagcaccca ggttcgcgcc tgaagcaata gcacaagcca gaagagcaag
    24781 ggtaaacgca ggcgtccgtg cgcatcataa tggacaggat ggaactcagt actcttcatc
    24841 ttcaatcaaa cgcttaccca gactcagcac gtcggcgtgt tcatatccca ggcgttcata
    24901 cattccgagc accatgtcgt tatcttccgg cacgttgatc tgaattttcg ggcagccgcg
    24961 agcaatcagc tttttctcca gccggttaag caacgcattg gcaattccac gcccacgaaa
    25021 ctctggatgc acgccaagat aataagcaga cccgcgatgc ccgtcataac cgcccatcac
    25081 cgttccgacc acttcaccgt ttacctcagc gaccaaaaac aaactgacgt catggttcat
    25141 cttacgctcg atgtccattt ccggatcgtt ccacggacgc agcaagtcgc aacgctccca
    25201 aagggtgatg acctcttcga aatcttcctg gcgaaatacg cgtatctcca tggtattcgt
    25261 tacctttttg cgggttaaaa ggctgattat ggcgtgaacg gtcgaattag ccaatatctg
    25321 acgaaaatcg gttgaaaaag tggcataatg gggagttgtc aactattgaa atgaaaagta
    25381 aaacaattct caacagcaaa ccgtcgtaac ggattacgcg atacgatata acatctggaa
    25441 ctttattatt acaactcagg ccgtatgagc acttttaaac cactaaaaac actcacttcg
    25501 cgccgccagg tgctgaaagc cggtttagct gccctgacgt tgtcaggaat gtcgcaagcc
    25561 attgccaaag acgaaccttt aaaaaccagc aacggacaca gcaagccgaa agccaaaaaa
    25621 tctggcggca aacgtgtcgt tgttctcgat ccaggccacg gcggaattga taccggagcg
    25681 atcggacgca acggttcgaa agaaaaacat gtggtgctgg cgattgctaa aaacgtccgt
    25741 tccattttgc gtaatcatgg gattgatgcg cgtttaacgc gttctggcga tacgtttatc
    25801 ccactttacg atcgcgttga aatcgcccat aaacatggcg cagatctgtt tatgtcaatt
    25861 catgccgatg gctttaccaa cccgaaagct gccggtgctt cggtatttgc cctctctaac
    25921 cgtggggcaa gtagcgcaat ggcgaaatac ctgtctgaac gcgaaaaccg cgccgatgaa
    25981 gttgccggta aaaaggcgac tgacaaggat cacctattgc agcaagtact gtttgatctg
    26041 gtgcaaaccg atactattaa aaacagcctg acgcttggct cgcatattct gaaaaagatt
    26101 aagccggtgc ataaactgca cagccgcaac accgaacaag ccgcgtttgt ggtgttgaaa
    26161 tcgccgtcgg ttccttcggt gctggtggaa acctcgttta tcaccaaccc ggaagaagag
    26221 cggctgttag gcacggcggc gtttcgtcag aaaatcgcca cagcgattgc tgaaggcgtg
    26281 atcagttatt tccactggtt cgacaaccag aaagcacatt cgaaaaagcg ataagttatg
    26341 aaacccgacg cacaccaggt taaacagttt ctgctcaacc ttcaggatac gatttgtcag
    26401 cagctgaccg ccgtcgatgg cgcagaattt gtcgaagata gttggcagcg cgaagctggc
    26461 ggcggcgggc gcagtcgggt gttgcgtaat ggtggtgttt tcgaacaggc gggcgtcaac
    26521 ttttctcacg tccacggtga ggcgatgcct gcttccgcca ccgctcatcg cccggaactt
    26581 gccgggcgca gtttcgaggc gatgggcgtt tcactggtag tgcatccgca taacccgtat
    26641 gttcccacca gccacgcgaa tgtgcgtttt tttattgccg aaaaaccggg tgcagagccc
    26701 gtttggtggt ttggcggcgg cttcgattta acccctttct atggttttga agaagacgcc
    26761 attcactggc accgcaccgc ccgtgacctg tgccagccat ttggtgaaga cgtttatccc
    26821 cgttacaaaa agtggtgcga cgattacttc tacctcaaac atcgcaacga acagcgcggc
    26881 attggcgggc tgttctttga tgatctgaac acgccagatt tcgaccactg ttttgccttt
    26941 atgcaggcgg taggcaaagg ctacaccgac gcttatttac caattgtaga gcgacgtaaa
    27001 gcgatggcct acggcgagcg cgagcgcaat tttcagctct accgtcgcgg tcgttatgtc
    27061 gagttcaatc tggtctggga tcgcggcacg ctgtttggcc tgcaaactgg cgggcgcacc
    27121 gagtcgatcc tgatgtcaat gccgccactg gtacgctggg agtatgatta tcagccaaaa
    27181 gatggcagcc cagaagcggc gttaagtgag tttattaagg tcagggattg ggtgtaactc
    27241 cctcaccccc attcccgcat ccgctgatgc aacgtcaacg actgcttctc ggcaaacagc
    27301 tgctggtaat ccgtggcaaa ttgccccaga tgccagaatc cccactgcat ggcggcgtct
    27361 tttaccgttg tgctttgcga ccacgggctt atcagttcgc ggcgtacggc gttcaggcga
    27421 atgcgtttca gccacgcgtt tgggccaatg cctaaaatag cgtgaaacgc gttttgtagc
    27481 gtgcggcgac tgacatgcag ttgattacac aaatccagca ccgtcaccgg ttcggacatg
    27541 ttttccagca catattcacg ggcgcgggaa agcaaccgac ggtaactctg atgactgatg
    27601 ctttccgccg tcaccattgg ttgcgcttct tccagcatcg cccccatcgc cattagcaaa
    27661 ttatccccca gcacttttcg cactgcaggc tgatggagat tttccgggtt ctcgcaaaac
    27721 gtcgccagcg cctgttggac aaagccccac agcgcggctt tatgctgctc tttcacttcc
    27781 agcgccgact ggctacgcag catatgtaat acccgatccg gattgtgcaa aaagttagcc
    27841 tgacaggtga tgacatcttc agacagcact acgcccagga tcgtataatc gtccggcgta
    27901 cttaattcaa actcggttcc accggggcgc gtcgctattt ctgcgcttcc cagacattgc
    27961 gaaccgataa atccctgctc accgcgcgtc gccggaatgc caaaccagaa cgagttcggc
    28021 cagaccaggc acgactgacg cagcgccaga ccggtgtatt cacgaaaaac ctgaatatca
    28081 tcgagtagaa tttccgtaaa ttcaccatga aacttgcccg gatgcagctg atcgtaaatc
    28141 tgctgctagg cggtaatcgt caaagcatgt tcatagacat ccgttgtctg tcgttgatga
    28201 acattatcca cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg ttcatgataa
    28261 agatggtgca aattggctgt acgggtcttt ttcatgatgt taatgccggg tgttgtagga
    28321 cacccggcac ctccgacagg ttaatggggc ttgagacgat aacgactact gcgtttacgt
    28381 aacgtcccgg cagaaaagag ctgttcgagc gcatttctgg ctttttccag cggccagcca
    28441 aagtgggcgg ccacttctcc tgccgtcatt ccctgacata ctgatgttaa cagcgccaac
    28501 aactcgtcag cagattccgc gacgataact ggcgcgtcgg gttcctttac gggttgcttc
    28561 ggctggcggt taaagccagt aaccagccac tgggtgtcat cgtctggccg accgatctcc
    28621 ttacggctga tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc cagcgccgca
    28681 cgacacgccg ccagatcgcc ttccaccacc agcgttaagc gaccagggtc aagcacttcg
    28741 tgactgagca gacgaacgtt agctgctttg agcatggcat ccgctgcatc tatcgcggcg
    28801 accatgccgt ccacttccag caatcccagt gcattgatca tcggcaacct ccgttacgca
    28861 cgctggattg gattacgcgc gatttccagc actgcatcgg taaaggcgtt acaggctgct
    28921 ttacacgcgg cctggctacc ggttaaaaat gctgccgagt agttggtttc agacggtggc
    28981 gggacgtagg tcaccagctg tacgtcggca gatttcaacg cggcatcaat gccgtaggtc
    29041 gcttccagcg gcggtgccac cagatacgcc atcggatcgc caagcgtgat cccggcggtt
    29101 gatgaaagat aagaaccggt acgcgaaacc acatgcgcca ggaaggcggt gttttctgcg
    29161 tcattggccc actggaaagc tgcgccattt tcaatatgtg caaccatcgc atccagaccg
    29221 gcacgcactt ccgccgggtt tggcccacca agcataatca gcacttcacc ggcagtcggt
    29281 gacgggccgt gtgccgctcc cgcatacagc gagcgaccat ataccacttc gaccatcgct
    29341 tgtttggtcg cttcgtcagc cgcaatgtag gttacgtcgt cagaatcggc agaaatcagc
    29401 ccgaggctac gaatatgcgg cggcaatttt agttcacgcg caaattcagc gttaacggag
    29461 gcaatcaccc gcatggcggt taccgacggt cgaatcaaat ctaaagctgg catgatgcct
    29521 ccttatcggg tcatgttgat gccggatgct ttctgctcca gcatacgttt cgccaaatcc
    29581 acaatgacgg cacagcagca cgaaagagaa gcaaaagtca gctacctggt acactaggca
    29641 gcgggtcagc aaaggggggc aaatgcccct tttttcatgt cagataatca gatcattacc
    29701 tcaataaact aataatcaca tcgcggttac aaccagtttg atagtgatag ctcaaaccca
    29761 gacgtggcgc tggctgcaag caaaataagc cgcaaaaatg cgaccccatg tttactagcc
    29821 gcaaaaatgc ggtcctattt gccagaacct tttattattc aattggttag tgcaagaaaa
    29881 agcgtgatta ctgttcaaga agtatccaga aaggtcatga ttttgacgct gaaaaaaaat
    29941 aggtgggcat ttttgcccac tcactagccg caaatttgcc cactcactag ccgcattttt
    30001 gcggcccaaa gtaataaata cagagaaata ataaatacag atcagcgcca agatttttcg
    30061 tcttttcggc ggaatcatga gactagtgct tacgtcctga taacctccag agcagacctt
    30121 aacttctgtg cagcaggact acgctcattg ccgctcggct aggtcaaatc ctcacgataa
    30181 agctgttcgg attgtgacca taatgttaaa ggactcttta aatcattcct tgacgaatta
    30241 actagctggc ttaagcggaa tagcatgcag gataaaaaca aaaccagctt agcagtgggg
    30301 agagcttcgc ttcgctcgtc tcctggggcc aatgctcaat gaaacgacgt aaggcacgga
    30361 gcgctcacca taggtagaca cataaacatc tgagagcgca ccttctatcg cgctacgcgc
    30421 tcaataaaaa ccaacgacac cgccacactg caagaccaat ggtttttatc gcgtatacag
    30481 accagacagg aaacagcagc aaagcagcgt atagccaggt gcagacagca cactcacacg
    30541 acgcacagca ggcttaagtg atcactcagg aaactttatc cccgccgccg atcaccgcga
    30601 actatcatga ccgcgccaaa ttcgaaagga atggcccata gaacgcgatg aacacacata
    30661 agcgctacac ttacttaacg tcgccacaga gcgattgaga gcgcttaaaa tcgatgcgat
    30721 ttcattaaat ttttctcact cgcctgaatg gtggcgatca ggactgatcg caacattgcc
    30781 ccgcctcgat attgagcgca taatagatac atacattaag cggaaaagag ataagtttat
    30841 tgtgaaaatc agggggttac atatcatgcg catatcagaa caacaacggc agatcctcga
    30901 ctatctggca aaaaacgaat caatcaccgc acgacaagcc gcagatctcg tttatggtgg
    30961 caatgttacc cgcgtccagg ttgacgccgc ccgccgctca ctcatgacga tggttagtaa
    31021 tgggctaatc aggaaacaag gccgcgcttt cgtagctgaa agacatatca atcacgctta
    31081 tagccaggac gttgaagaat tgcgccgcca gatcctttgg gtattcagca aacagggtca
    31141 ggagtatttc ctttctgaac acccaaattt cgccgccaaa gagttaacag taagcggatt
    31201 gtcgtgcgca ctggattatc ttgtcggtga aggttgcgtt acttacaagc agctatggca
    31261 cgccctgaat acgcttgtag aagatggttt actcattgcc acgcgtgaga acatcgaata
    31321 ctggataatg ccgcgttcag gtcgccgcaa tcatgcacgg tatatttcag gcaacagata
    31381 cagaacgccg gactttaatc cgacacagga agatcgggac cgtgccgaaa tgatgtgcag
    31441 ggaacaggag aaaagaatta tggataagtt attttcgatg cagtgaacac aagcgggcgc
    31501 aatgcccgtt tttattgcct tattccacgc ctgacgaatc aatttgatca cacctgagaa
    31561 aatgcatagc cagaagttaa ggccgttata ttgcgattgg gagcgtttta aacggtatca
    31621 atttaattaa ttctgatgtg gtgatttgca ggcaaaaaaa acccgccgga gaagcgggaa
    31681 atagagacat atcgttgtag ttattaacag atcactttga ggtcgagggc ggctaaccct
    31741 cgctaatatt atcccgctat taccttgcgg gtgcaaataa tcttttattg ctttgatgat
    31801 ttaataaata gcactttttg ctaacaaaaa ctgctgtcaa tatatggcca aaaatgtcaa
    31861 cagaaaaaat aaggtttata atattaagta tcacatgggt aatgggttca gggaatcgga
    31921 atccaccgcc tcacggaaat tcaaaatttg cttcttcttt cctccgtggg cggcttcttt
    31981 ttcgtatcga ggtatttatg aaagcaataa cattagaaca gttcgcagca atggcgaatg
    32041 tatcgaccag aacgctgagg cgctggctaa aaaaaccatc aaccggagtc cctttgccat
    32101 ttaccccacg cggtactcgc caattattct gggagcacga atgcaaagaa tggttgtaca
    32161 gaacagaacg ataataatca cgcatcacat tttgaggtaa acatcatgac caatatcgaa
    32221 agattagaac gctgtgccga tattatgcgg cgcagatgga tttatgaccc tgaagcgggt
    32281 ttattgttta gccgtgaaac aaaagacgtt atcaaaggca gcatgaccaa taaagggcat
    32341 ttggttgtaa cggtacatga aggtaaattc aaagtgacac tcacctatca gaaagcctgc
    32401 tatgtataca catacggcgc ttatgacgag acattatatg aagtgcacca cgttaatttg
    32461 aacaaacagg ataaccgcat caagaatatt cgcctgatgc cgaaagaaaa gcaccgccgg
    32521 atacataaaa acgtccgcaa acttattcgg ctgggccaca cacaatggct tcaaccgcaa
    32581 ttaattgaac aatcactata aacacaggag taaataccat gcattcatta gacagttatt
    32641 ttcagcgcac gacagcccct aaatcagccg cccaggaacg ccgtgaggaa ttccatgaga
    32701 aagtgatgcg ttctgctgat tatattgcgg acaagttcgt ggaaacggtt cgtccacttg
    32761 ttgatgaagt agctgataaa cttcagtctg aaatgccgga agatatggaa ggcactgcaa
    32821 agcggcgact catttgcgaa ttaagtcgtc gttttggcgt gtcaataagc gcttttaaat
    32881 aatcgaaaaa aggggggaaa gataatggac gcatttgagg tactggcaaa taaaggcgct
    32941 gaactggtag cacagcgtga taaaaccgcc aacgaagggg aacgcgcact gctaaataaa
    33001 cagattaaag cgatccgtat ggcccagttt aaattaatca gtaatgaagt tatcgaaaca
    33061 attaaaccgc agatcccaca aatcgttgct gacgctatta aagccgccgg actggagaaa
    33121 cgcattgccg ggctaaaaac gaacgcagag aaaatggaga tgcacaaaca gagcggtgct
    33181 ccgaatcccg atgagtactt ctcaccggat gttgagttta tggcgcaggt tgaggaacgt
    33241 ctgggtagct gtctcaccga ggaacagcgt cgctattttg atggagtgga tagttcggca
    33301 ggtattgacc tgaactcgta tttcggtcgt gaaattgagc attttgatgc cgccccgtct
    33361 atgccggagc cagaaccaga aatggccacc gatcctgaag ctgagcaacg gaaacgcgta
    33421 ttcaatgcga tagggattcg gtactgatcg gataaaagaa agccgggaat taccccggct
    33481 gacttaacaa tgtgaccaat gaacaaatcg gacaagacaa acaggataag cagtctcgaa
    33541 acaatgttcg ggaactatat tagcacaagt ccggagaaaa tcatcatgtt ggcactacgc
    33601 atcatgcagg gcatcgctaa gaccctcgca gaacatgtct tagacctgaa acattcgcca
    33661 ctgagcaaac aggccatgaa gcgccaaacg cttcgtctgt gggctgaata ttcgcttgga
    33721 actatcaata aaatcatcga catgaaatcg gggccgagca accagagcgc ggaggaaatg
    33781 gagttcatca ggcgtttaat tctcattcgc agagacattc atagccagtt acacagcgtg
    33841 ggaatcgata ttaatgatgg gacgggcgat taattttcgg acaagtacta tgcgcgcgcg
    33901 aggagctgaa catgaagcaa gatattgcag acaggctgga gatactcgaa ggccagcggg
    33961 cagaagcgaa acaactacgt aagcaggcca gaagagcgca caggaacaac gaagcagagt
    34021 tactaaccaa atacatcagt ttcaccaatt actgcatcta cgagtgttac aaggaagatg
    34081 cagaggactg gctggattca ttgccagaac agtactgagc agaataacac cacgtcatgt
    34141 tgttctgagg tgagcatatc acctctgttc aggtggccaa atttagtgtg ccacttcagc
    34201 atcaccgttg aaatcacaat aacgattttt ttgtaaatat gctctcacat atgtaggaat
    34261 agaccaggtt ggcattgaaa cacatctacc atgaccataa taaagtaatt ttttgatatg
    34321 tttatggttt tatattaatt aaaatatatc taaaattgat agttttaaaa ttgcaaaatc
    34381 cagattgtgt ttgtaatgtt taatctgatt gtattgagtg gcgtttatta tgaaaaataa
    34441 atcaggtaat tcttgttata gagaagcagt cgatattcat agctcacttg agctttcatt
    34501 tatattgcat aaatatcatt tgcgtgtaga tgagaagaaa tctcgaaatg tattcagcgc
    34561 catacgtgat gagctcatcc ttgatgggga ttcacgacaa aacttagcta ctttctgtca
    34621 aacatggctt gatgatgaaa tccgtgagtt gcttaattta gctattgata aaaacatgat
    34681 ttataaagat gaatatccac aaatggcgga acctgaaggt cgctgtgtgc gtatgctttc
    34741 cgattcgtgg agtttccccg attcgcgaca caccttaggt tgctcaacca ttggttcatc
    34801 tgaagctgct atgctcggcc gcttggcgtt aaaatggcag tggtgtaaaa aacgcgaagt
    34861 acaaggtaaa tcaaccgaac ctgatttgcg atcctgtgca aatatgctgg cataaattct
    34921 gccgttactg tgatgtgaaa ttgtgtgaaa ttccacttga tgatcatcac ctgataatga
    34981 atgctaaaga ggttttgaag catgtcgatg aaaacactat tggtgtcgtt acgactttag
    35041 gtattactta tttttgtaaa gcagattgcg ttagtgtttt taagataagt gttaaagtta
    35101 atattagttt ttatatttac atcgtaatta tatgtttcta ttttaaaaat gttcgagagt
    35161 ataaaaatgt ctaatgctaa tatcgcaccc aaaagtaaga aaatgacagt ggggacactc
    35221 gctatcatga atataactgc tgtagtaagt ctacgattta taccttctga agcagaatac
    35281 ggcttaggtg ccatttttta ctataccttt gctgcaatta tgtttttagt acctgttgcc
    35341 tttgttgcgg ctgagctagc tacaacatat ccacaaaaag ggggggcatt tcgatggata
    35401 ggagaagcat ttggggctcg ctggggtttt gtaggaatgc ttatgacctg gctacaagtc
    35461 atcccctact ttccaacagt actgacattt ggtgcagtct cggttgcatt tattgatgtt
    35521 gatatggggg tggcagaatc aattgccagt aataaatggt atattttatt tttcgtacta
    35581 tttgtttact ggggagctat ttgtgttgca ttacgtggcg ttggtatttt ctcgcaggta
    35641 tctaagtggt gcggtattat agggacaatt attccagcta ttatccttgt tattcttggt
    35701 ttctcctatc tgtggttcag cggtaaacca gcggtgatcc cattaggttg gggtgatctt
    35761 ttccctaata tagctaactt ccagaatatt gttcttgccg ccagcatatt tcttgcttat
    35821 gcagggatgg aaatgaatgc ggtccatgtt aacgatttag ataatccaac taaaaaatac
    35881 ccaattgcaa ttaccatcgc ttctataggt actattgcaa tattcctgct cagtacttta
    35941 ggggtcgcct ttattattcc tacagataaa attagtctaa ctcaaagttt gttggttgcc
    36001 tacgatatgc tgttcgaatg ggctggagtg ccatggcttg gctctgtgat ggcatttatg
    36061 ctagcaattg gcgtactggg tggggtggtt acttggattg cagggcctaa tacaggtgta
    36121 ttagcgatcg cgaaagctgg ttatttacct aagtttttcc aaaaaaccaa tcgtcatgga
    36181 atggggcacc atctaatgtt tgtacaaggt ataattgtct cagttctttc tgttactttt
    36241 gtgataatgc cttcagttca agcagctttt cagattttat cacagttaac agttatgctc
    36301 tatttagtta tgtatatgct catgttcgct agtgctatct atctacgaca ttctcaacct
    36361 gatacaaaac gcccatatcg agcccctgct ttatttttct gggcaacact tggattctta
    36421 gggtcttttc tggcttttgt attaagtttt attccgcccg agcaaatccc aactggtagt
    36481 ccaaccagct atgttcttta tttgttagta ttagttgccc tatttttatt aattccaata
    36541 ttaatttacg ccttccgaaa atctgactgg aaagatccaa actctgattt cgaaccattt
    36601 tcttgggaaa aaagaactag taattaaatt ttccataagc tacaaaagat gaggttaaat
    36661 atgtttagtg ctgaatctcc aattggtgta atagactcag gtgtaggtgg attgacatcg
    36721 gtaaaggaaa taattaattt gcttcctggt gaagatatta tctattgtgg agataatatg
    36781 aatgcacctt atggcaaccg ttctgccgat gatatcatca gtttgactaa aaaaatgctg
    36841 acttttttac agtcaaggaa tgtcaaacta gttgctgtcg catgtaatac tatatcctca
    36901 actttggaat ccgaagaata cagcggatat gctaagagct ttccgtttcc tatcttatcc
    36961 atcattgaac ctgcagtaga agatgtgatt cgccagcaat ataaaaatgt tggtattatc
    37021 gctactgagt ttactattaa aacggggtgt cacaaagagc taataaaaaa attgaattca
    37081 acaataaatg tatttggaga accaagcaaa aatcttgcaa tgttaattga ggaaggtaat
    37141 cttaatgctc ctgcaatctt gaatgacatc aaaaaacatg ttaaccattt aatttccctc
    37201 catccagtaa atgaaattat tctcggctgt actcactatc caattgttca gaattttttt
    37261 gaagcagttg caccggatat aaaatttatt aatcctgcac atgatcaggc tatctctatt
    37321 aaaaatcact tgggacagct aaatttatta aactcctcaa atataggaac tttgcaaata
    37381 aatactagtg gaagtatgga gatctacaaa acggtactag gggaattgag cattacgaaa
    37441 cctcatacat tcagtatcag gcagttttaa atttatccgg tgcaccgtaa aatcccgtcc
    37501 ttctgaactg caccccaaaa gttggacacc aactgagtaa ggtgcatttt atggacaaac
    37561 ccagttacat actgggtagt gcatccaatt agtagaacat gtgtttttcg ataaacgctc
    37621 cgatcacttt ttcgtggatc tcaactgagc gtgagaagca gattgtttta cgacccaacc
    37681 gcttaatgcg ggtgcgtagc gtcagattat tacgctcaat gcgttgggtg aatattttgc
    37741 cggtcagatg cttattcttc ggcacctccc ggccatagct gccccagtca tcgctggtca
    37801 gcatgccgat gttgaagggt gtaagcagtg ccagtagctc ccggcacgtt tgatcggttc
    37861 ggggaccaaa agtgtaggcc agtacaccgc ctgttttggt gttgtacgcg tacccgagcc
    37921 aatgttgccg ggctttactg ccaacgtagc tccattgctc atcaagctcg cagataaacg
    37981 ccacatcagc atgggcaaca ggcgaagaag ttattcgctt tggcgcgagt tttttaaagt
    38041 ccggatgacg gtgttactac caattttcag tgtcctggcg gtatcgcgaa ccccggcacc
    38101 attaaaggcc atttcagtta tcagctcttt catacccggc ttacgtgctt gataagtata
    38161 agtgaactga aacacacggt ggcagtcacg gcagcgaagt ctgtcacggc ctttagggtt
    38221 ctgaccatgg cggtaaacct gagctgactg acaacgggga caatgaatat taacgctggt
    38281 catgagataa cctcaaaagc ccgtattata catcagattc aactaattag aggcatcacc
    38341 catccagcat tataaaacat actaacaata actaaattat aataatcaca acttacaatc
    38401 aaataataaa tgcataagat taacaatgat tttttcgggt tatatttatc taaaaaagca
    38461 gaatattatg taacttatta ttttctaagc gaaaatttat tgtgcggtca gactaacaaa
    38521 cttgcgtacc acccgaagaa catgcgaaga tttaagtatt ctttcttttt tggtttttat
    38581 ttttcatttt ctcttttctt ctctttccgg ttctgttctg gttttggttt tttccttttt
    38641 atttttattc tttcttcagg gttatgctaa aacaccaaaa catttttcac cacaaaagca
    38701 tccaatcatc ttgccagacc aatcacctta accggataca ctgccggata taatcccact
    38761 aacacccaga caccaagcac catacctgat aactaccgta ttcaggaagg aaatgcgagt
    38821 gatttcaccg tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag
    38881 tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg ggagttgttg tagctgtggg
    38941 cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg ccaccaccag caggggatgc
    39001 ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag aaccgcggcg
    39061 taacgcacag gcagcgaggc gggaagcgcc agcacgtaca cgtcagtttt gggatcaaga
    39121 tccagctgaa gcagcgcctg ttgccaggca ctcaccagat ccgtttcatc aatcgggatc
    39181 gccaccggca aattgcgcag cggcagttgt acgccctcca gccagattgt gctgccagag
    39241 agcgaaaggg tatgtgcacc cgcgccaatc accgtggagc gtacggtttg cgccggaaac
    39301 tgcacattca tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag aagtgggcca
    39361 atatcggcaa aacagaacgg gttggcgggc tggtggcgat aacattcgcc cacgcctcca
    39421 gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca aaccggtttg catcaatgcc
    39481 tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa tcagcgccgc catccgccgg
    39541 gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt cagtgcctgc accgaagcac
    39601 tcatccacaa tcatctgccc cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt
    39661 tccagcaggc gaccaccgac gttgagacag gcagtgccgc tgatttttcc ggcatcgaac
    39721 agggcgtagt tcgcggtgcc accgccgata tcgatattca gtacccgaca cagccgttgt
    39781 tcagaaaggg tttgcgcccc ggctccgtga ccggcgatca cggattcgag gtgcggcccg
    39841 gcgctggcga cgacaaaatc gcccagcgac tgtttttcct tcatcacttt ttccatcagc
    39901 tgtgcgacca ggctttcggt aaactggcct tccggcagct gggcaatgat ggtttcgcgg
    39961 atgcgctggc tttcggtttt gtcggctggc acgctggcgg gcacggacgc cgttaccggg
    40021 atcgattcat cacattcggt gatggtaaaa cccagtagat ccgccacttc acgggcttcc
    40081 ggggtaataa tgctggcgcg cagaaccacg gacattgcct gttcgccgcg cgcgtgtgct
    40141 tcacgaatat cattcgctgt gatgagtttt ttcacctgct tcctcctgtt tggtgagaga
    40201 tgccagataa tccaccagtt gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat
    40261 cggctcttcg aaacccgtct caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg
    40321 catgtccgtt ttactgatga cggcgatttg ccgtttgctg acgccaatat ccagcaaccc
    40381 ggcaggcaac cgactttccg ggtcatttgc gccatggaca tagatcagca tgtctacatc
    40441 ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg ctaaaatatt cacccggcgt
    40501 atcaatatcg cccttatcat taaattccac ggccagtgtt tttctggcga gggtataatt
    40561 cccctgcaac gcattaaata acgttgtttt tcctgccccg acgctaccca caaacgcaat
    40621 tcgtttcatg aatg
//
LOCUS       DADLCQ010000021        40315 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000021 DADLCQ010000000
VERSION     DADLCQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.021"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            231..1664
                     /locus_tag="JQG95_001016"
     CDS             231..1664
                     /locus_tag="JQG95_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY5887658.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JQG95_001017"
     CDS             complement(1672..2730)
                     /locus_tag="JQG95_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5887659.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JQG95_001018"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JQG95_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JQG95_001019"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JQG95_001019"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY5887660.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQG95_001020"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JQG95_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY5887661.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQG95_001021"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JQG95_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY5887662.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JQG95_001022"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JQG95_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY5887663.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JQG95_001023"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JQG95_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY5887664.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQG95_001024"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JQG95_001024"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY5887665.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQG95_001025"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JQG95_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY5887666.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQG95_001026"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JQG95_001026"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY5887667.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQG95_001027"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JQG95_001027"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY5887668.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JQG95_001028"
     CDS             complement(17699..18322)
                     /locus_tag="JQG95_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY5887669.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JQG95_001029"
     CDS             18619..18894
                     /locus_tag="JQG95_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887670.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JQG95_001030"
     CDS             complement(18971..19213)
                     /locus_tag="JQG95_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY5887671.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JQG95_001031"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JQG95_001031"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY5887672.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JQG95_001032"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JQG95_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY5887673.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQG95_001033"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JQG95_001033"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY5887674.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="JQG95_001034"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="JQG95_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /locus_tag="JQG95_001035"
     CDS             24093..24827
                     /locus_tag="JQG95_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5887675.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="JQG95_001036"
     CDS             24840..25697
                     /locus_tag="JQG95_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5887676.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQTQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFSLTP
                     GEYSARFQG"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQG95_001037"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="JQG95_001037"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY5887677.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQNILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQG95_001038"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="JQG95_001038"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY5887678.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVIGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQG95_001039"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="JQG95_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="JQG95_001040"
     CDS             complement(30355..31602)
                     /locus_tag="JQG95_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY5887679.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="JQG95_001041"
     CDS             complement(31624..31950)
                     /locus_tag="JQG95_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY5887680.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQG95_001042"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="JQG95_001042"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY5887681.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="JQG95_001043"
     CDS             33338..34594
                     /locus_tag="JQG95_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY5887682.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="JQG95_001044"
     CDS             34709..35035
                     /locus_tag="JQG95_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY5887683.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /gene="mntH"
                     /locus_tag="JQG95_001045"
     CDS             complement(35176..36414)
                     /gene="mntH"
                     /locus_tag="JQG95_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY5887684.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="JQG95_001046"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="JQG95_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY5887685.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="JQG95_001047"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="JQG95_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10752 a        10025 c         9312 g        10226 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatata gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aagtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaagtca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacccgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccatgataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tatcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt tctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ctagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 cttcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttc aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatatttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccctat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaggt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagctaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgacg
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctggtt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttg
//
LOCUS       DADLCQ010000022        39966 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000022 DADLCQ010000000
VERSION     DADLCQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.022"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(74..1327)
                     /locus_tag="JQG95_001048"
     CDS             complement(74..1327)
                     /locus_tag="JQG95_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY5887686.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1559..2890
                     /gene="argA"
                     /locus_tag="JQG95_001049"
     CDS             1559..2890
                     /gene="argA"
                     /locus_tag="JQG95_001049"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY5887687.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSLHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2952..4778)
                     /gene="recD"
                     /locus_tag="JQG95_001050"
     CDS             complement(2952..4778)
                     /gene="recD"
                     /locus_tag="JQG95_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY5887688.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4778..8320)
                     /gene="recB"
                     /locus_tag="JQG95_001051"
     CDS             complement(4778..8320)
                     /gene="recB"
                     /locus_tag="JQG95_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY5887689.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="JQG95_001052"
     CDS             complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="JQG95_001052"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY5887690.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11377..14745)
                     /gene="recC"
                     /locus_tag="JQG95_001053"
     CDS             complement(11377..14745)
                     /gene="recC"
                     /locus_tag="JQG95_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY5887691.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14758..15090)
                     /locus_tag="JQG95_001054"
     CDS             complement(14758..15090)
                     /locus_tag="JQG95_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY5887692.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVMIVTALSGIQRTSMNSL
                     ASRNQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMT
                     RLHCPNRQ"
     gene            complement(15075..15482)
                     /locus_tag="JQG95_001055"
     CDS             complement(15075..15482)
                     /locus_tag="JQG95_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY5887693.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15479..16042)
                     /locus_tag="JQG95_001056"
     CDS             complement(15479..16042)
                     /locus_tag="JQG95_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY5887694.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16033..16503)
                     /locus_tag="JQG95_001057"
                     /pseudo
     CDS             complement(16033..16503)
                     /locus_tag="JQG95_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16687..17481)
                     /gene="thyA"
                     /locus_tag="JQG95_001058"
     CDS             complement(16687..17481)
                     /gene="thyA"
                     /locus_tag="JQG95_001058"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY5887695.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17488..18363)
                     /gene="lgt"
                     /locus_tag="JQG95_001059"
     CDS             complement(17488..18363)
                     /gene="lgt"
                     /locus_tag="JQG95_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY5887696.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPDSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18514..20760)
                     /gene="ptsP"
                     /locus_tag="JQG95_001060"
     CDS             complement(18514..20760)
                     /gene="ptsP"
                     /locus_tag="JQG95_001060"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY5887697.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGSANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20773..21303)
                     /gene="rppH"
                     /locus_tag="JQG95_001061"
     CDS             complement(20773..21303)
                     /gene="rppH"
                     /locus_tag="JQG95_001061"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY5887698.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINLQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21988..22677
                     /gene="mutH"
                     /locus_tag="JQG95_001062"
     CDS             21988..22677
                     /gene="mutH"
                     /locus_tag="JQG95_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY5887699.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22746..23459
                     /locus_tag="JQG95_001063"
     CDS             22746..23459
                     /locus_tag="JQG95_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY5887700.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23597..23815
                     /gene="ygdR"
                     /locus_tag="JQG95_001064"
     CDS             23597..23815
                     /gene="ygdR"
                     /locus_tag="JQG95_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY5887701.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23923..24963
                     /locus_tag="JQG95_001065"
     CDS             23923..24963
                     /locus_tag="JQG95_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY5887702.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRCQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24996..26186)
                     /gene="lplT"
                     /locus_tag="JQG95_001066"
     CDS             complement(24996..26186)
                     /gene="lplT"
                     /locus_tag="JQG95_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY5887703.1"
                     /translation="MSESVHINTSLWSKGMKAVIVAQFLSAFGDNALLFATLTLLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVVIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26179..28338)
                     /gene="aas"
                     /locus_tag="JQG95_001067"
     CDS             complement(26179..28338)
                     /gene="aas"
                     /locus_tag="JQG95_001067"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY5887704.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28924..29955
                     /gene="galR"
                     /locus_tag="JQG95_001068"
     CDS             28924..29955
                     /gene="galR"
                     /locus_tag="JQG95_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY5887705.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29962..31224)
                     /gene="lysA"
                     /locus_tag="JQG95_001069"
     CDS             complement(29962..31224)
                     /gene="lysA"
                     /locus_tag="JQG95_001069"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY5887706.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31346..32281
                     /gene="lysR"
                     /locus_tag="JQG95_001070"
     CDS             31346..32281
                     /gene="lysR"
                     /locus_tag="JQG95_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY5887707.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32268..32960)
                     /gene="ygeA"
                     /locus_tag="JQG95_001071"
     CDS             complement(32268..32960)
                     /gene="ygeA"
                     /locus_tag="JQG95_001071"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY5887708.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33089..34507)
                     /locus_tag="JQG95_001072"
     CDS             complement(33089..34507)
                     /locus_tag="JQG95_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY5887709.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGGLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34822..35583)
                     /gene="kduD"
                     /locus_tag="JQG95_001073"
     CDS             complement(34822..35583)
                     /gene="kduD"
                     /locus_tag="JQG95_001073"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY5887710.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTAQGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYAIAVDGGWLAR"
     gene            complement(35613..36449)
                     /gene="kduI"
                     /locus_tag="JQG95_001074"
     CDS             complement(35613..36449)
                     /gene="kduI"
                     /locus_tag="JQG95_001074"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY5887711.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36736..37917)
                     /locus_tag="JQG95_001075"
     CDS             complement(36736..37917)
                     /locus_tag="JQG95_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY5887712.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38283..39401
                     /locus_tag="JQG95_001076"
     CDS             38283..39401
                     /locus_tag="JQG95_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5887713.1"
                     /translation="MVLFITALVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYY
                     GKKIGNLITTLYFIAFFVVVLIYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILN
                     LIFLMGRHATIRVMGFLVFPLIAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWIS
                     IPVMVFAFSHTPIISTFAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLS
                     IPSSYIEAAKEEGVTILSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGAT
                     EVVKTTLQQVGVKKSRAFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILF
                     IMPTLSTYLIPALKPWRSIGNLITLIVGILCVSVMFFS"
BASE COUNT         9915 a        10804 c         9701 g         9546 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctgatct cccataaaaa aagcgccatt
       61 cagcgccttt ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa
      121 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      181 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      241 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg atttttccga
      301 gcttattcag taccgcttta ccaaacttaa ggctgtcagc aatagtcagc gactgcacca
      361 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
      421 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg
      481 agagcgcaaa caccgaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag
      541 agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
      601 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctcttcctcg atcagagagc
      661 gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg
      721 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
      781 ctgctttacc cggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt
      841 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
      901 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
      961 gttttacgtt ttgctttaat tcaaaaacca tgcgtacggt ttgcgggtca aattgcccga
     1021 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
     1081 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga
     1141 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1201 gccagacgcg caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca
     1261 atagccacat agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1321 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
     1381 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
     1441 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
     1501 tgcgaaaaaa cagaataaaa atacaataat ttcaaataat catgcaaagg ggtgtgccgt
     1561 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     1621 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     1681 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     1741 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
     1801 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     1861 gggaacattg caactggata ttactgctcg cctgtcaatg agtctcaata acacgccgct
     1921 gcagggcgcg catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt
     1981 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgct
     2041 ccatcgtcaa ctggaaagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
     2101 tggcgagagc tttaatctga cctcggaaga gattgccact caactagcca tcaaactgaa
     2161 agctgaaaag atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat
     2221 tgtctccgaa cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg
     2281 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2341 cgtgcgccgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     2401 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     2461 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     2521 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     2581 gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
     2641 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     2701 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     2761 cacgcgcagt cttcactggt tccaggaacg tggatttacc ccggtggata ttgatttact
     2821 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     2881 tttagggtaa gcgatgaaaa tcaccggatg cggcaggcgt aacactcgtg cgtcgcatcc
     2941 ggcaattacg tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3001 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acgacgacgc
     3061 gcgcgggtca ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt
     3121 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3181 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3241 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     3301 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag
     3361 tgcggatgac gatgaatttt gcgcttctgt tgcataaact gttcaattcg ctcattcagt
     3421 ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc
     3481 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc
     3541 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg
     3601 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
     3661 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     3721 cagagactgt cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc
     3781 gtcaggcggc ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag
     3841 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     3901 atcactcgcg catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
     3961 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4021 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
     4081 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
     4141 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4201 tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct
     4261 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc
     4321 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
     4381 tcaacctcaa tggtatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac
     4441 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc
     4501 ctgctgaccg cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
     4561 ctgacacagg tcgccaacag cggattcgac gcctcgttat tttccagtcg tgaaagcggc
     4621 aaacagacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
     4681 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
     4741 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
     4801 cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc
     4861 cttgttgcgg atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt
     4921 gaagatcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat
     4981 aaagctgata ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg
     5041 tgtaagccga actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc
     5101 cttcgtggcg gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact
     5161 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
     5221 tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt
     5281 cggaaagctg actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga
     5341 tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct
     5401 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
     5461 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     5521 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     5581 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     5641 gtagtgtcct cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat
     5701 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
     5761 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
     5821 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
     5881 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac
     5941 gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa
     6001 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6061 accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat
     6121 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6181 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa
     6241 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     6301 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     6361 tgcgccagat ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt
     6421 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
     6481 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct
     6541 gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca
     6601 gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga
     6661 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
     6721 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     6781 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     6841 cacctttaaa tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
     6901 gtatttcgcg aaacatgaac gtgtcatcag tctggctgaa aagcttgttc acactgttca
     6961 ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac
     7021 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
     7081 cgccaatcag caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt
     7141 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7201 tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
     7261 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     7321 cagccaatgc gcgggtgatc aacagatcgc ggatcgacaa tggttctgca agcagttgat
     7381 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta
     7441 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
     7501 ctgcccaggc gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     7561 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     7621 gttttaccgt atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat
     7681 cgggcggcgg tgctttgata accggcggtt cgccttgcag ataacgatta atatcgcgca
     7741 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
     7801 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
     7861 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
     7921 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
     7981 accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat
     8041 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8101 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacgatca
     8161 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg
     8221 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     8281 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     8341 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     8401 tcggctttcc cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg
     8461 accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc
     8521 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     8581 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
     8641 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
     8701 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     8761 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
     8821 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     8881 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
     8941 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
     9001 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
     9061 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
     9121 aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
     9181 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9241 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
     9301 agctgctctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc
     9361 ggcagacgct gggtgtaacc attagcatta atcataaggc cgttgttagc gttggtggaa
     9421 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
     9481 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
     9541 aaaatcaggc tgacatcagc tttgggctcg ctggcaaaat aacggcttgg cgcatacacc
     9601 acgcgcagat tcgactcatc aacaatcagc tctggatggt cgtatttctt ctctgactta
     9661 atcagcgaga aatcatcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg
     9721 gcggcttttt tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc
     9781 gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc
     9841 gcattctgcg gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg
     9901 gcaatattga ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc
     9961 cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca
    10021 ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag
    10081 ctaaaaattg ccgccacaac ctgatcgcga ttagccaagc ctttatcagt taaagacgca
    10141 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    10201 tcaactaatc cctgcttttg cagccagtca gaaagtgtac ctgggctgcg attgccaatc
    10261 agataggtaa tcaattcatc ggttttacta cggaacttcg ctgagttgtt atcgatgcga
    10321 aactcaacgc gcaacacttt acgcggcagc gcagggacgt aatgaatgat aatgcccttt
    10381 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    10441 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt actgtaaata
    10501 accgccttca tcaaattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    10561 accggattac caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    10621 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    10681 cgcgccatgg ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg
    10741 agcaagggtt ctgcaatagc atcggccagg cggtctaccg caccgggcaa ggcgtcgttc
    10801 tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg
    10861 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    10921 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    10981 acgggcacta ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    11041 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11101 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccagagggca
    11161 acaaacaaca ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa
    11221 ctttattttc tggacagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg
    11281 tgctgcacga gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    11341 agtaagcgga tagattgcgc aatttttata caacactcat gactgattaa agcgaaacag
    11401 cggtaacagg aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg
    11461 ccgccagagc cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    11521 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    11581 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    11641 caatggcgcg gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    11701 ctgttcggct gcaagcggcg gaaaacgcca ctcaccgtct ttgcgtagaa aaaggcgact
    11761 ttcgccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    11821 cgccacactt aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    11881 ccagccagtt atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg
    11941 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa
    12001 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg
    12061 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    12121 aagtccttcc agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tatgaaagtt
    12181 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12241 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    12301 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    12361 ctggtagttt tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac
    12421 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    12481 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    12541 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    12601 caggtagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    12661 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    12721 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    12781 gagaaaacgc tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag
    12841 tgacagcggc accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    12901 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt
    12961 gagcatatcg cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg
    13021 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13081 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13141 gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    13201 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13261 ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    13321 atccagcagc gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaggct
    13381 gataaacgct tccagtaccg gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg
    13441 caggtaacga tccgcaggtg cgctaccaaa cactgcctga ataaacggac tgtagctgtc
    13501 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcggggctt cctccagcat
    13561 cgccagcaag cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    13621 tgtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    13681 gatgttcaca cccgcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg
    13741 cagcaggtta tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc
    13801 agaaaggaga taaatgtagt cgcgcccaag cttcccccat gaggccagca gcgagttgcc
    13861 gacatcctgt tcaccatcgc tgttaaagag ctgtccggca ttttcgctat cgcgaaatag
    13921 cggcaattcg cgatcttcaa aactgtgtcg acgctgacga gtcagcagtt tcgccagata
    13981 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14041 ttcaatatgt ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat
    14101 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14161 ttcgataaag cgctgataga gattcgcgcg gtgccagcgc ggttgtccga gttcatgggt
    14221 atattccacc agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc
    14281 aaccaaatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    14341 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    14401 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    14461 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac
    14521 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    14581 accaaacttt tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag
    14641 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    14701 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    14761 ctgacgattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    14821 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    14881 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg
    14941 gtactggttt ctgctggcga gactgttcat tgatgttcgc tggatacccg ataacgcagt
    15001 gacaatcatc accatcagca ccatcagcac catcgcaacc attacctccg gcaggctaaa
    15061 gccttgttga ttcttcaggg aagctgacat aatgccccct ctttcagcgg acaaaaatcg
    15121 ctccagccgc gtggcgaaaa aacaattttt ccatcaatga cttcgcctgt tcgccacaac
    15181 gaaacgcctt cataaccggc aatcaataag gcttcattat ctgccagtaa acgcaaacaa
    15241 acctgggcat cggttccagc gtactgcgag cactgaactg ctggctgcgt ctgccaggag
    15301 tgcatttttc cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt
    15361 tccatgctca cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag
    15421 ctacccaaaa ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc
    15481 acaggttgaa tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac
    15541 tggcagcacg catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga
    15601 cctgaaaagt gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt
    15661 cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct
    15721 ggtcggactc ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca
    15781 caatgataca gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac
    15841 aataacccgc cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt
    15901 ccagtgccag cttacgagtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg
    15961 cagcccccag caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa
    16021 aaccttgctc ttttacaggc atccttctgt ttctccttgc tgataaagcc ggagtcttcc
    16081 ccacggcgaa accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc
    16141 ccatgcggta ttacgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac
    16201 ttcgggccag cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc
    16261 agcggaactc acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg
    16321 gttatgccag ttggcatctt cacgtaaata gagcaaatag tcccgcgcct agctggcggt
    16381 ttgccaaagc cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag
    16441 catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt
    16501 catgccggca gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc
    16561 gcatcttccg gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg
    16621 aagaatttaa cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt
    16681 tcgtaattag atagccaccg gcgctttaac gcccggatgt ggatcgtagc cttcaatctc
    16741 aaagtcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg
    16801 cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatggt tgctgtacag
    16861 gtgcgtatcg ccaccggtcc agacaaaatc acccacttcc agatcgcact gctgcgccat
    16921 catatgcacc agtaacgcgt agctggcaat gttgaacggc aggccgagga agacgtcaca
    16981 ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaatgc
    17041 atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat
    17101 gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc gtagtaatct ggtcaatatg
    17161 acgaccatct ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc
    17221 attttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt
    17281 agtatcgccc tgcaggaacc acagcagttc gtggatgatg gaacgcaggt ggcaacgttt
    17341 ggttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga
    17401 aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat
    17461 cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata
    17521 tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat
    17581 gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac
    17641 aatgatgcga aacgcgccgt agccaatcag gaacaagcct gagacagctc ccattgggcg
    17701 cggtttacga atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagttc
    17761 gtaaagctgt gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg
    17821 cgggttggtt tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa
    17881 cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc
    17941 ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca gagacctgga agaaggaacg
    18001 tttagtacgg cgggcgaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa
    18061 agacatgccg ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa
    18121 attgtagaac agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata
    18181 gagtaagttt tcaacttcat ttttggtcca gccactgtcc ggacgattcg cccgacgtgt
    18241 tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc
    18301 cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt
    18361 catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc
    18421 cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc
    18481 ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc
    18541 catgccgcga cgctccataa aggctgcaac ctgatgacga acttcggtcg ccagttgcgc
    18601 ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa tcaatgcgcc gcagcaggta
    18661 ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag
    18721 gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacgaagat cgataccatg
    18781 tatttccgct tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc
    18841 gtaaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc
    18901 gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat
    18961 tgacggcact tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc
    19021 ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt catcgaggct
    19081 ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc
    19141 ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc
    19201 caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc
    19261 cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc
    19321 cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata
    19381 aagaccgatg ccatcaatac agctgcccag tttttcttca tgttccgggc ttaaaccagc
    19441 attgagcatg acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc
    19501 ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag
    19561 taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg
    19621 atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac
    19681 catgatcgca gcatgggagt tggctgagcc atctcgcacg acaacaccga ctaagcgatc
    19741 ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc
    19801 cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag
    19861 cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc
    19921 ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc
    19981 ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat
    20041 agccgccgtt tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt
    20101 tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga
    20161 tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc
    20221 cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacagggc
    20281 agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga
    20341 ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa
    20401 ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga
    20461 ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag
    20521 cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg
    20581 ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta
    20641 gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc
    20701 ctcattcagg cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat
    20761 aattggcgtg acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt
    20821 attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc
    20881 acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc
    20941 aaactctggt gtactgctgg tttgcagatt gatttctgca tcgccgctta ccagctgcaa
    21001 gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg
    21061 tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg
    21121 gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg
    21181 gttgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac
    21241 ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat
    21301 caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga
    21361 cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac tcacagttat
    21421 gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga
    21481 tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc
    21541 gatatagaaa ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaatg
    21601 aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac
    21661 caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg
    21721 gagtttcgtc gcacgtcaag gctgtaaatg aaaacagtag tggaggtttt tcacagttat
    21781 cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg
    21841 aaaacctatt gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca
    21901 gagaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac ataattactg
    21961 tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc
    22021 gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa
    22081 ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt
    22141 ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt
    22201 gctgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa
    22261 acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc
    22321 catgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc
    22381 ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac
    22441 cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag
    22501 cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc gaatgcgaaa
    22561 gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt
    22621 tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca
    22681 tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt
    22741 tttagatgtt atttgcatgg ataaccgatc ctaacgcttg gcttgcgctc ggtacgctga
    22801 cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa
    22861 agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta
    22921 tgcgtctggc gctgctggca tcaatctctt gggttacgcg cctgacaaat ccgcttttta
    22981 caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt ggcttgttcc
    23041 ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga
    23101 aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct
    23161 tcagcctcga ctcggtgatt accgctgtgg gtctgtcaga tcacctgttt attatgatgg
    23221 cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg
    23281 tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta
    23341 ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt
    23401 tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat ccgctctgat
    23461 actccgtacg ctctcctgcg ggagggcgta ctcactcctt ttaccctcac agtttaagat
    23521 tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca taggtaggcc
    23581 aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt
    23641 tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt
    23701 gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca
    23761 aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat
    23821 aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca
    23881 tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata
    23941 ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag
    24001 aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt
    24061 atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa
    24121 acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc
    24181 aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat
    24241 cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac tgattacctc
    24301 gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat
    24361 agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag
    24421 taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta
    24481 atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac
    24541 ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa
    24601 ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc
    24661 aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc
    24721 ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctt
    24781 gatcctgctc agatggcgct cgcgtttgta cgctgtcaac cgtttgttgc cagcactctg
    24841 ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta
    24901 agcgaagacg tattagctga aattgaagcg gtacatcagg tttatactta tcctgcacca
    24961 taaagattaa cgccggttaa aaccggcgtt aaatattaat gacggcgctg ccagatccac
    25021 agcgccgtta ttgccagcgc aaatagcgca ccgaagccaa tgccaatggg cacgaccggg
    25081 atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc gctgttttcg
    25141 ccaaggtttt gtactgcaat cgcattcccc gccccgacgc tttttttacc ccgctcctgt
    25201 agcaacgcat tgagcggaac gacaaaaaag ccccccagca cgccaatcag catcaacaag
    25261 gcataggctg gcagcagctc gtgttgcagg gagaaaatcg gtaccaccac gccaatcaaa
    25321 atcccggctg gcatacagcg tgacacggtt tccagcgtaa ccagcttcgc tgccgcacct
    25381 gcgccaacca cgataccaat cactaccatc gcgttgagat aggtcggcgt agcgttatcg
    25441 gtaatgccca gcgccaccgg tacccacagc accaacagga aacgcagcgt gacacccgct
    25501 ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgca ccatagcgag
    25561 gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat tccaggactg ccccggacgc
    25621 gccgccagtt tgggaatgta gatattggca acgaccgcac caccgtaggc cagtgcgcat
    25681 gcggccagag cgaccaggac atgccagtca gccagcacac caccggctac ggaaccgagt
    25741 aaaatcgccg ctatggcaga agcttccatt aaaccgttag cttttactaa cttactaccc
    25801 gtggttaatt cgccgagaat accgtatttc gccggtgaat aggctgcagc accaacaccc
    25861 accagcgtat agccgagaaa cggattgata ccaaagcaga tactggctgc gcccagcagc
    25921 ttcaggccgt tggcaaacat catcacccgg cctttggcga agctatccgc cacctgcccg
    25981 acaaacggcg ccaaaagaat gtaagcacct acaaacacca tttgcaggat gggctggctc
    26041 cactccggat agaactgcgc tttcagtaac gtcagagtgg caaacagtag cgcgttatcg
    26101 ccaaacgcag agagaaactg cgccacgata accgctttca tccccttcga ccacaacgaa
    26161 gtgttaatgt gcactgactc actcatcgtg ttgttccgct tcgtctaccc agcttttcaa
    26221 cgtgacaaag tcaggtttgc cgctgccaag taatggcatc tgtttcagat agcgaatatc
    26281 gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca tactgttgca acttatcgcg
    26341 cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg catcgctctt
    26401 aatcgcagtg gcatggactt tatctggcga aacaccaagt gccagttgtt ccaccatttc
    26461 cagcgacacc atttcgcctg caattttggc aaagcgtttt gcgcggccct gaatctgcac
    26521 aaagccctgc tcgtcaaaac gcacaatatc gccagtgtca taccagccgc gctccatttc
    26581 gccgcgaaca ttctcggcgg tgggcacttc cagtacacct ggcttctcca cccgcagata
    26641 gccgttcatt atgttcggcc ctttcagttg caggcgtccg ccctcttcga taccagggac
    26701 cgacaacagg cgtgcatcca ttcctggtag aatacgccct accgtaccgg gtttcgccgc
    26761 catcggtacg ttgatagaaa cgacaggcgc gcattcggtc acaccgtagc cttcaaggat
    26821 gcgcaggcca aatttatcct gccaaagctg tttggtactt tcttgtagtt tttctgcgcc
    26881 tgccaccaca tagcgtagac gatagaagtc atacgggttg gcgaaacgcg cgtagtgacc
    26941 gaggaaagtc gaggtaccga acaacacggt gcaactgcgg tcatacacca agtccggcac
    27001 aatgcggtaa tgcagcgggc ttggataaag gaacacttct gcacctgtaa gcagtggcgt
    27061 aaacaggcct accgtcagcc caaaggagtg aaacagcggt aatgccgaca taaagcgatc
    27121 gttggtggtg aagtcggcaa tcgttttaat ctgctcgacg ttcgccagaa tgcttttatg
    27181 gctatggacg acgccttttg gatggccttc agaaccggag gtaaagagga tcagcgcctc
    27241 ttcttccggc tgctgtttta cctgcgccag gcgcggcatc agcaaatgag cgaagatcca
    27301 tactttgtcg gcagtggtga catctgcttt taaatcttcc agatagaccc agcgcacctg
    27361 agtaagttgc tccggcagat gccagagttt gcctttatcg agaaactggc gggaagtgaa
    27421 gatggttttg atttcagccg ccgtaatagc actggtcagc ccttttaccc cggcagtgta
    27481 gttcatcatt gcgggaatgc ggcgacgcgc gatggcccca aaaatcactg ccgcactgat
    27541 gcctgcattg ggcagcatta agccgatgcg ttcgccttca acactgtatt tttcaaggat
    27601 gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag gagtctgggg taaagttgac
    27661 gtcttcgaca catttcttcc cggctccgaa gcggtacatt gcactcagta aagattcgta
    27721 cagagtttca cgcgggcgca ccgccatacg cgcttccatc attatttgat gcagcatttc
    27781 gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc ggcatcgcca cctgcgttgg
    27841 aggcaaaata tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac ctttcaggcg
    27901 gctgaagtgc gtaagttccg ccccttcaat acgcacagga ataaccgttg caccagactt
    27961 cgccgcgaca aaacccgcgc catcgtagat tttcatcagc gagcctgtag tggtgatgcg
    28021 tccttcaggg aaaatcacca ctggtcggcc ctgttccacc agacgtacca gatgtttaat
    28081 agccataggt tgcgtcgggt cgagaggaac aaagtcgata aatgatttca gccaacgcat
    28141 ataccactgt tggcttattg aggtgtaaac ggcaaacact ggacgcacag gtaaaaacag
    28201 tccaagcaaa atgccatcaa taaaagagac gtgattaggc gtaattagta cgcgctcgcc
    28261 cttcagtgcc tgggtgtcac ccgtaacgcg aacgcgatac aaaacacggc acaaatttcg
    28321 aaaaaagcta aaaagcattc caactccctt tgctctgatt cagtaaaagc gaatggaggg
    28381 agattacacg agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa aaacctgcgc
    28441 atccgcgcag gttggtgcaa gagacagggt acgaagagcg taccgaataa tctcaccaat
    28501 caatacctct gggatcttga ttgtggtctg cacgacgact cttcgccagc gagaaaacgc
    28561 aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt acagattgat
    28621 gaccggcaaa aaaaaacctg cgcatctgcg caggctggtg taattcatgt gctcaacacg
    28681 aagttgactt cacctatcaa tacctctggg atcaccactt tagcaacctg aagccaaacg
    28741 ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt
    28801 tttttgtctt acttatttgc gtttggctca cattcccacg atgaaaacac gccacccctt
    28861 gaaccaacgg gcgttttccg taacactgaa acaatgtaag cgtttaccca ctaaggtatt
    28921 ttcatggcga ccataaagga tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc
    28981 cgcgtcatta ataattcacc caaagccagc gaagcttccc ggctggctgt gcatagtgca
    29041 atggagtctc ttagctatca cccgaacgcc aacgcccgtg cgctggcgca gcagaccact
    29101 gaaacggtcg gtctgatcgt tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa
    29161 gcggtcgaac aggtggctta tcacaccggt aatttcttat tgattggcaa cggttaccac
    29221 aacgaacaaa aagagcgtca ggctattgag caactgatcc gccatcgctg tgctgcgttg
    29281 gtcgtccatg ccaaaatgat cccggatgcc gatttagcct cattaatgaa acaaatgccc
    29341 ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa accgttgtat tgctcttgac
    29401 gatcgttacg gtgcctggct ggcaacgcgt catttaattc agcaaggtca tacccgcatt
    29461 ggttatctgt gctctaacca ctctatttct gacgccgaag atcgtctgca agggtattac
    29521 gatgcccttg ctgaaagtgg tattccggcc aatgatcggc tggtgacatt tggcgaacca
    29581 gacgaaagcg gcggcgaaca ggcaatgacc gagcttttgg gacgaggaag aaatttcact
    29641 gcggtagcct gttataacga ttcaatggcg gcgggcgcga tgggcgttct caatgataat
    29701 ggtattgatg taccgggtga gatttcgtta attggctttg atgatgtgct ggtgtcacgc
    29761 tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg taacgatggc gacccaggct
    29821 gccgaactgg ctttggcact ggcggataat cgccctctcc cggaaattac taatgtattt
    29881 agtccgacgc tggtacgtcg tcattcagtg tcaactccgt cgctggaggc aagtcatcat
    29941 gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc agtaattctt cgatggtctg
    30001 gcgacggcga atcaaccgcg cctgaccatt atcaaacaga acttctggta acagcggacg
    30061 gctattgtag ttggatgaca ttgatgcgcc atatgcccct gtatcatgca gtaccagata
    30121 atcacctgcc ttcacttccg gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt
    30181 aaagacatcg cccgattcac ataacggccc ggcgacgacg gtttccaccg ttggcgcgtg
    30241 ttccagagaa cgaccatcag ctgccagggc actgatatgg tggtaactac cgtacattgc
    30301 cgggcgcatc agatcgttga acccggcatc aaccagcaca aagtggcggc tacccatttg
    30361 tttgacgctc cgcacctgag taattaatac gccagactgc gctaccagga agcgacccgg
    30421 ttcaatttcc agtttcacag ggtggcccaa atggcgagcg atttgctcac gcgcggcatt
    30481 ccacagacca taataatgtt cggtatcaac cgcctcttca ccctgttgat aaggaataga
    30541 aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg aattcgataa cctgacgcac
    30601 catagcacca cacacctgct ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg
    30661 aatgccgact agctgcagat gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt
    30721 gtaccagata ccgtgcttgc tgttttcgcc accggtattg gttttttggc tatgtccgtg
    30781 accaaacccc ggattaacgc gcagccatac ccgatgccct ggcgaaacct ggcccagttg
    30841 gtcgagcgta tcaacagaac ccgcattcac cggaatttgc aattcactga cgcgttcaag
    30901 cgtcgcctga tcgataacat ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt
    30961 gtaacccgcc gccaacgcac gctctatttc gcctaacgag acggaatcca ctttcacgcc
    31021 ctgctcacgc attaagcgca aaatatgaat attggaacag gctttctgtg caaagcgcac
    31081 cacttcaaac tgtttcagcg ctgcaatctg ccgacgaata atttgcgcat cgtagaccca
    31141 caccgggcag ccaaattcag cgggcaaacg cagcagattt tcggcggtga gatcggtatc
    31201 ggtgctgaac agtgaatgtg gcataacaaa ctccagatga gtgctttttt atgattacgc
    31261 cacatcataa aaagaataaa aaatatcgat ttatgtcgag tctatgcaaa aatgatatgg
    31321 attaccggat tgcgagagag cgctaatggc cgccgttaac ttacgtcata ttgaaatttt
    31381 tcatgcggta atgaccgccg gaagcctgac tgaggcagca cacctgctac acacctcaca
    31441 gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg atcgggctga aattgtttga
    31501 gcgcgtacgt ggacgattac atcctaccgt gcaaggactg cgtctgtttg aagaagtgca
    31561 acgatcctgg tacggactgg atcgcattgt cagcgccgca gaaagtctgc gcgagtttcg
    31621 ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg caatcttttt taccgcagct
    31681 cctgcaaccc tttctggcac gttatcccga tgtcagctta aatatcgtgc cccaggaatc
    31741 accgctactt gaagagtggc tctcggccca gcgtcatgat ttaggactca ctgagacact
    31801 ccatacgcct acgggaacag aacgtaccga attactctct ttagatgaag tgtgtgtgtt
    31861 acctccgggt catccgctgg cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga
    31921 gaattacatc agcctttccc gtactgacag ctatcgccag ttgctggatc agctatttac
    31981 tgaacatcag gttaaacgac gcatgatcgt agaaacccac agcgccgcgt cagtctgcgc
    32041 aatggtacgg gcgggggtag gtatttcggt ggttaacccg ctcaccgcac tagattatgc
    32101 ggcaagcggt ttagtggtgc ggcggttcag tattgcggtt ccgttcaccg tcagcctgat
    32161 ccgccccctg caccgcccgt catcagcgct ggttcaggcg tttagtgggc atttacaagc
    32221 ggggttaccg aaactggtca cttctcttga cgctattttg tcgtcagcta cgacagcata
    32281 aaagcgacag catcctcggc atggatcgcc gcggtatcaa acacaggcaa aacactgcgc
    32341 tcttctggca ccagtaaacc aatttctgtg cagccaaaaa tgacgccctg tgcgccctgt
    32401 tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt aaattgcccc
    32461 agacacagtt cttcaaaaat aatctgatta attttcgccc gttcatccgc ttcaggaata
    32521 agacaattga tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg
    32581 taacgcgtac ccagcagcgc cacacgagtc attccggccc cggtaattgc acgtccggtg
    32641 gcatccgcaa tgtgtaagaa aggcagagtg caacgtgact caatggcatc cgccacttta
    32701 tgcatcgtat tggtacataa cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc
    32761 gcctcagcca gaatgtcccc ggttttatcc cattccccgc gacgctggca ctcttctatt
    32821 tcatgaaaat cgacgctatg tagcagcatt tgcgcagagt gaagcccacc aagccgctgt
    32881 ttaatgcctt catttatcag acgatagtaa ggaatggtgg attcccagct cattcctccc
    32941 agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt taccgcaaca aggctgaata
    33001 aggaacgaga caaacgcctc aacggccagg tgcccaatct ctattaacga aaaaagggcc
    33061 ggatgtacag cacatccggc ccgtgaaatc agacgccgat atttctcaac ttctcgcctg
    33121 ccatcagttt gcgttcgata tgttccagcg tgacattttt ggtttccgga atgagccaga
    33181 aagtaatacc cacaaacgca atgttcagcg cagtgtagag ccagaacgta ccggcagcgc
    33241 caatgctatc aagcagtgtc aggaaggccg cgccgataat catattcgac acccagtttg
    33301 tcgtcgtcga acaggtaata ccgaaatcgc ggcatttcag cggctgaatt tcagagcaca
    33361 ggatccacac cactggcgcg gcgctcatcg cataaccggc aatacacatc atcgtcatgc
    33421 caacagagag ccaggacaag ccactggaag ccgtaccgtt atcaaactgc atcaggcaat
    33481 agcccagcac cagagtgcct aacgccatca cgctgaaacc aattttcaga gccggtttac
    33541 gacctgcttt atctaccgta aagaccgcaa tgaaggtcgc gaacataaag gtcagtccca
    33601 cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt ttgaagatac
    33661 gcggcgcgta gtacatgatg atgttcatac cggtaaactg ctgcatcgcc tgcaacaaca
    33721 taccgagaaa cacagcacga cggacgttac ggttgatctt aaacagtgcc caaccgccct
    33781 gttttaactt caggctttca cgaatttcgt tgagttcttc tcgcgctttt tccgacgtat
    33841 cgcgcagcat acgcaacact tcttccgcct caatatgacg ccccttttcc gccagccagc
    33901 gcgggctatt tggcaggaag accaccagaa taatcagcag aactgctggt aaagcaagac
    33961 cccccaacat tgcgcgccag ttaccgctat aactgaacgc tgtatcggat aaaaacgcca
    34021 gcacgatgcc gagtgtgacc atcaactggt acatactgat catcttaccg cgaacgtttt
    34081 cacttgccat ttcagaaaga tacagaggag cggtgtaaga cgcgatcccg acagcaatgc
    34141 ccagcaccac acgagcggcg attaacatct ctacgctggt cgcaaaagcg gaccctatag
    34201 aaccgagtac aaacaggatg gcccccgcca tcaggctgta tttacgcccc aggcggaacg
    34261 acagccaacc attaaacagc gcaccaattg ctgcgccgag catcatgcta ctaaccaccc
    34321 attcctgcaa acgactggtc agcacaaagt gatcggtaat gaacggcaac gctccggcga
    34381 ttacgccgat atcaagacca aataacaatc ctgcgaccgc agcagctacc gaaacaaaca
    34441 tattcatacg ccgcgtatca cgcaaagaac gtggcgttaa agcagattcc gtattgatag
    34501 taaccatttt ttcctgccag cagagagtaa gacattgtga aaaaatacgt gaacaattca
    34561 cacaggtgtc aggtcggaaa cagcataaat atggattaaa ttgctgcgac atgccgttat
    34621 gtgatggata ttccaatttt aaaattaagt tgaattattg agattattat taacctccta
    34681 attttaaagc agataaaatt cataaagttc attaattgat aattgatatg gattatttca
    34741 taaccatgat atggattatg atgatctaga gatataaaaa accctgccat gcggcagggt
    34801 cataaaagta agaggaatga attaacgcgc cagccaaccg ccatccacag caatggcata
    34861 accatttaca taatctgaag cgctggaggc aagaaacact accggcccca tcaggtcact
    34921 cggcagtccc caacggccag ctggaatgcg atcgagaatt tccgcgctac gttgttcatc
    34981 tgcccgcagt tgttgggtat tgttggtcgc catgtaaccc ggagctatcg cattaacatt
    35041 aatgttgtgt ttagcccatt cgtttgccat caatcgcgtc acacccatca cgccgctttt
    35101 tgatgcggta taagaaggca cacggatccc gccctggaag gagagcattg acgcgatatt
    35161 gataatcttg ccgccattgc cttgcgcgat aaagtgtttc gccgctgcct gagacatgaa
    35221 gaatacgctc ttgatattca ggttcatgac atcgtcccag tctttttcgc tgaactcgag
    35281 agcgtcttcg cggcgaatca atccggcgtt attcaccagg atatcaatat gaccaaattc
    35341 agctaccgcg cgatccagca gtccaggaat gccatcaatc tttcgcagat cggcggtcag
    35401 gcttaaaaaa cgacgcccct gcgccgtgac ctgctcgatg gtttcagtcg gttcaacgat
    35461 gttaatgcca acaatgtcac agcccgcttg cgccagcccc aacgccatcc cctggcccag
    35521 cccagtatca caaccagtga cgaccgcaac tttaccttgg agagaaaatg cacttaaaat
    35581 cataacaaca ccttattcgt ttatgcccac aactagcgca aatctttaac gcccacatgg
    35641 tccatatcat taaagacctg gttttcaccg accatgcccc agataaaggt ataagctttg
    35701 gttccgacgc cggaatggat cgaccagctc ggggagatca ccgcctgctc gttatgcatc
    35761 acaatgtgac gcgtttcttg cggctgcccc atcatgtgga aaacgcaggc gtcatcatcc
    35821 atattgaaat agaaataaac ttccatccgg cgctcgtggg tgtgacacgg catggtgttc
    35881 cacaagttac ctggagccag ctccgtcagc cccatactca attgacaggt ttccagtaca
    35941 tccgggacaa aatatttgtt aatcgtgcga cggttactgg tgaggttatc gcctaacgtg
    36001 actggagata cttcgtccgg tgtgactttt ttggtgggat acgtcgtatg cgcgggtgcg
    36061 caattgtaat aaaacttcgc cggagtggcg gtatcaatac tggcaaagac aacttctttt
    36121 gcacctttac caacatacag ggcgtcgcgg tgaccgattt catagcattg accatcgaca
    36181 gtaatcgtac cggcaccgcc aatattgata acacctaact cgcgacgttc aaggaaatag
    36241 cttacgccga gttgtttacc aacttccccg ccaacggaaa ccgttttagt tatcggcata
    36301 atgccgccaa caataattcg gtcaatgtgg ctgtaaacca tggtgtactc atcggcgaca
    36361 aatacctttt caaccaaaaa ttcattgcgc agcccttggg tatccagcgt ttttgcgtgc
    36421 gcaccgtgga tgctctgtct tacgtccaca tcaacctccg ataatcagtg aagcgtgaaa
    36481 atagaaaacg atagtgaaac actgtttcgt tttctctata aagggatgat atgcccatgg
    36541 gctagggttt tcaattaaaa ataaaacaat gttttatttt taatttaagg attaaaaaaa
    36601 gtgcagtgtc gatcacgaat atgtcatgag aatggaaaaa tgagggagcc cagaaaatgt
    36661 atgtcgactt gccagccatc attattcaac agattaatga cggcagatat ttatcggtaa
    36721 ggaggaaatg cagagctact cgtcacgttc aatggtcaat gccacgccct ggcccccgcc
    36781 gatacaaagc gttgccagcc ctttgcgggc attacgtttc accatttcat gaaccagaga
    36841 aaccaggatt cggcaaccgg aagcgcctat cgggtgaccg agtgcgatcg cgccaccatt
    36901 gacattgacc cgacgctcat cccactcaag catcttgcca accgaaagcg cctgtgcagc
    36961 aaacgcttca ttagcctcga taagatcgac atcagccaac tgccagccta cacgctccag
    37021 gcaacggcgg gtcgcataca ccggcgcaat tcccatcaat gccggatcta caccaacgct
    37081 ggcaaatgcg cggatgcggg ccagcacggg taaattcaac gctcgtgctt tggcttcgct
    37141 catcatcatt accgcagctg cgccatcgtt tatggatgat gcattccccg cggtcacaga
    37201 accgagacta tcaaatgaag gatttaaacg ggctaaacct tctgcactgg cgtcagtgcg
    37261 tggctgttca tcggtatcaa caaccaacgt ctgaccgtta ctttggatca ttaccgggac
    37321 gatctcatct ttaaatcgtc cggcgtcaat cgccgctcgc gctttttgtt gcgagctaag
    37381 tgcgtaagca tcctgcaact gacggctgat gccatattcg cgagccagat tttcggcggt
    37441 gacaccaata tgataatcat tgaaggcatc ccacaaccca tcatgcacca gactgtcaac
    37501 caactggcta ttgccaagct gtgcaccggt gcggctatca gtcagaacat gtggtgcgcg
    37561 gctcatgttt tcctggccac cggcgatgac aatatcagcc tcgccacact gtatcgcctg
    37621 agtagccaga tgcagtgctt taagaccgga accgcaaacg tcattaatcg tgattgcaga
    37681 aacgctgtta ggaagaccac ctttaatagc cgattgcctt gccggattct gccctgcccc
    37741 tgcagtcaac acctgaccaa gaattacttc atccaccgca tatgcaggaa cgccggtacg
    37801 ttctattaac gctttcacga ccagactacc aagttccacg gcggaatgac ccgctaacgc
    37861 accacgaaag cagccgatag gtgtccgtaa cgccccgaca atcacaacgt ctttcatcac
    37921 aacctcgcac caattaacag tgcaatagta aatgattgtt aattacagtt atcttaattg
    37981 tttaaaaaaa gtgattttta tcacaaagga aatatgcctg agcagtagtc agagtcataa
    38041 ctggcacgta aggtttgcaa ccactaaccc accaatagat gggtagatag ggcgttaatc
    38101 tcccatactt aacttggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat
    38161 acgttcagat aatgtctaat atttggtcta aagaagaaac tctgtgaagt ttcgcgctct
    38221 atggcacagc cgttgatgca ggcacgctat tccttcctat tcagttaggt tcggcagggg
    38281 ctgtggtcct gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag
    38341 ccttatgcca gttcatcctc tcatcaaaaa catcagcagg tgaagggata acgggcgcgg
    38401 taacacacta ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct
    38461 tttttgtcgt cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg
    38521 caaagcatat ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa
    38581 ttctgaatct catttttctg atgggacgtc atgccactat tcgggtaatg ggatttttgg
    38641 tattcccatt gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac
    38701 ctgatctatt aacaacccag gtagagttca atcagaatac ccttcaccag atatggatat
    38761 cgattcccgt gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag
    38821 acagacgtga aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg
    38881 cttatctcat catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc
    38941 catcttcgta tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta
    39001 tgctgccgaa cgccccagca tggttgtcaa tttccgggat tattgtcgca gtagttgcga
    39061 tgtcgaaatc attcctgggt acgtactttg gcgttattga aggtgccaca gaggtcgtca
    39121 aaacaacact acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccac gcactatcaa
    39181 ttatgttggt atcgctgatt accttcattg tttgttgcat taacccgaac gcgatttcga
    39241 tgatttacgc gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgctgt
    39301 caacgtatct catcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga
    39361 tcgtgggtat cctgtgcgta tcggtaatgt tctttagcta atatccgttg tggatggttc
    39421 aaaattgcta caattttgaa ccattccatt tttcataata ttcatttgtt attattccgc
    39481 cctatcactt ctctcgtcat atcctagaat tatcagttag ttaaacacac aaagagatct
    39541 gaacatctta aataagacta tttaagatgc ataacttaga ttcgcaagat atcttctggt
    39601 cattcaaaaa caatttgcga tatagatatt ctcataccat gagatagtta tctggaccac
    39661 gttcgaccat gcaagattaa atcagtgaaa cataatcata tttaataacg agaaatgctt
    39721 ttttaaatat cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa
    39781 aattacaaaa tcaaggtgta ttgacatcat caatcaccaa tgaaattatg caattatata
    39841 cggataggga ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata
    39901 gttgctcaat gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa
    39961 actcgt
//
LOCUS       DADLCQ010000023        39745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000023 DADLCQ010000000
VERSION     DADLCQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.023"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..156
                     /locus_tag="JQG95_001077"
     CDS             <1..156
                     /locus_tag="JQG95_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5887714.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            385..975
                     /locus_tag="JQG95_001078"
     CDS             385..975
                     /locus_tag="JQG95_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5887715.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1363..3036
                     /gene="uidA"
                     /locus_tag="JQG95_001079"
     CDS             1363..3036
                     /gene="uidA"
                     /locus_tag="JQG95_001079"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY5887716.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS"
     gene            3072..4097
                     /gene="uidB"
                     /locus_tag="JQG95_001080"
                     /pseudo
     CDS             3072..4097
                     /gene="uidB"
                     /locus_tag="JQG95_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4136..5401
                     /gene="uidC"
                     /locus_tag="JQG95_001081"
     CDS             4136..5401
                     /gene="uidC"
                     /locus_tag="JQG95_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY5887717.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5627..7135)
                     /locus_tag="JQG95_001082"
     CDS             complement(5627..7135)
                     /locus_tag="JQG95_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY5887718.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7236..8411)
                     /gene="manA"
                     /locus_tag="JQG95_001083"
     CDS             complement(7236..8411)
                     /gene="manA"
                     /locus_tag="JQG95_001083"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY5887719.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8610..10256
                     /locus_tag="JQG95_001084"
     CDS             8610..10256
                     /locus_tag="JQG95_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY5887720.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10399..11802
                     /gene="fumC"
                     /locus_tag="JQG95_001085"
     CDS             10399..11802
                     /gene="fumC"
                     /locus_tag="JQG95_001085"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY5887721.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11799..12728)
                     /gene="tus"
                     /locus_tag="JQG95_001086"
     CDS             complement(11799..12728)
                     /gene="tus"
                     /locus_tag="JQG95_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY5887722.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="JQG95_001087"
     CDS             complement(12804..14105)
                     /gene="rstB"
                     /locus_tag="JQG95_001087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY5887723.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14284..15603)
                     /locus_tag="JQG95_001088"
     CDS             complement(14284..15603)
                     /locus_tag="JQG95_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY5887724.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPQLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="JQG95_001089"
     CDS             complement(15828..16571)
                     /gene="rstA"
                     /locus_tag="JQG95_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY5887725.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16700..17035
                     /locus_tag="JQG95_001090"
     CDS             16700..17035
                     /locus_tag="JQG95_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY5887726.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17032..17754)
                     /gene="folM"
                     /locus_tag="JQG95_001091"
     CDS             complement(17032..17754)
                     /gene="folM"
                     /locus_tag="JQG95_001091"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY5887727.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17791..19173)
                     /locus_tag="JQG95_001092"
     CDS             complement(17791..19173)
                     /locus_tag="JQG95_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5887728.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19359..20303)
                     /locus_tag="JQG95_001093"
     CDS             complement(19359..20303)
                     /locus_tag="JQG95_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5887729.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20827..22359
                     /gene="pntA"
                     /locus_tag="JQG95_001094"
     CDS             20827..22359
                     /gene="pntA"
                     /locus_tag="JQG95_001094"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY5887730.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22370..23758
                     /gene="pntB"
                     /locus_tag="JQG95_001095"
     CDS             22370..23758
                     /gene="pntB"
                     /locus_tag="JQG95_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY5887731.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="JQG95_001096"
     CDS             complement(23783..24817)
                     /gene="tqsA"
                     /locus_tag="JQG95_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY5887732.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTWTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25228..25593
                     /gene="mdtJ"
                     /locus_tag="JQG95_001097"
     CDS             25228..25593
                     /gene="mdtJ"
                     /locus_tag="JQG95_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY5887733.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25580..25909
                     /gene="mdtI"
                     /locus_tag="JQG95_001098"
     CDS             25580..25909
                     /gene="mdtI"
                     /locus_tag="JQG95_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY5887734.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25948..26769)
                     /locus_tag="JQG95_001099"
     CDS             complement(25948..26769)
                     /locus_tag="JQG95_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY5887735.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26869..26952)
                     /locus_tag="JQG95_001100"
     CDS             complement(26869..26952)
                     /locus_tag="JQG95_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887736.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27045..27380)
                     /locus_tag="JQG95_001101"
     CDS             complement(27045..27380)
                     /locus_tag="JQG95_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY5887737.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27777..29030)
                     /locus_tag="JQG95_001102"
                     /pseudo
     CDS             complement(27777..29030)
                     /locus_tag="JQG95_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29137..30039
                     /locus_tag="JQG95_001103"
     CDS             29137..30039
                     /locus_tag="JQG95_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5887738.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30174..31394
                     /gene="mlc"
                     /locus_tag="JQG95_001104"
                     /gene_synonym="dgsA"
     CDS             30174..31394
                     /gene="mlc"
                     /locus_tag="JQG95_001104"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY5887739.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31519..32214
                     /gene="bioD"
                     /locus_tag="JQG95_001105"
     CDS             31519..32214
                     /gene="bioD"
                     /locus_tag="JQG95_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY5887740.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32167..33459)
                     /gene="clcB"
                     /locus_tag="JQG95_001106"
     CDS             complement(32167..33459)
                     /gene="clcB"
                     /locus_tag="JQG95_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY5887741.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALVG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33618..34232)
                     /gene="dmsD"
                     /locus_tag="JQG95_001107"
     CDS             complement(33618..34232)
                     /gene="dmsD"
                     /locus_tag="JQG95_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY5887742.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34275..35129)
                     /locus_tag="JQG95_001108"
     CDS             complement(34275..35129)
                     /locus_tag="JQG95_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY5887743.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35131..35748)
                     /gene="dmsB"
                     /locus_tag="JQG95_001109"
     CDS             complement(35131..35748)
                     /gene="dmsB"
                     /locus_tag="JQG95_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY5887744.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35759..37797)
                     /locus_tag="JQG95_001110"
                     /pseudo
     CDS             complement(35759..37797)
                     /locus_tag="JQG95_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37858..39024)
                     /locus_tag="JQG95_001111"
     CDS             complement(37858..39024)
                     /locus_tag="JQG95_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY5887745.1"
                     /translation="MLIQIGRPRERTGWICSIVMVNVSHIRTLSSVESTTSSPSIRLT
                     VESIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLADLMT
                     VEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRT
                     QEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTP
                     SGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKS
                     RTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRIL
                     PGVSAMGQGAWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38950..>39745
                     /locus_tag="JQG95_001112"
     CDS             38950..>39745
                     /locus_tag="JQG95_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5887746.1"
                     /translation="MTDINHDDTAYPSSSFSGSPYLYQQVYTSSPDTRLTQIKDLATP
                     QLVRRTNGRYTVRFRLKGATSPFLSVSTRSTDRRTATMRQRELAATAKAFLLDNPEVT
                     LQELTAHIRSMAEQFLTDASDDYWNGVEVATLVDEKSNLRELAATRALSVDQQRGIKL
                     ALEVLTAAQQRVDTGDTSGLIKLIDGGVSQLTDDNNHTDYSTIGDSTSILKNEQGDRP
                     AVFTQERQLLTPESRESSVLFQELVTSLLAEKVQTLKQSSFKDLSAS"
BASE COUNT        10119 a         9634 c        10173 g         9819 t
ORIGIN      
        1 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggccaacc gcaagatatt
       61 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      121 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacggt
      181 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      241 gcagccaaat cacggctatt ggttaaccaa ttgctgggtg aaaagtatac gaatagagtg
      301 taccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      361 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      421 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      481 tcgatgaaag ccatctgtaa atcttgcgcc attagtcctg ggacgctcta tcaccatttc
      541 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggct
      601 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      661 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
      721 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
      781 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
      841 gcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
      901 gaagacctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtata
      961 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1021 tcgtaatttc tctgtgcaaa aattatcctc cccgtgttcg gagaattccc ccccaaaata
     1081 ttcaccgtag ccatatgtca tgagagttta tcgttcccaa tactctcgaa cgaacgttcg
     1141 attgcttatt ttatggcttc tgtcaacgct gttttaaaga ttaatgcgat ccatatcacg
     1201 ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1261 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1321 ttaaaggttg accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa
     1381 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1441 aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac aagaaagccg ggcaattgca
     1501 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1561 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1621 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1681 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     1741 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     1801 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     1861 tatgccggga tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     1921 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     1981 gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     2041 ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     2101 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2161 ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     2221 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2281 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2341 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     2401 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2461 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2521 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2581 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     2641 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     2701 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     2761 cgttattacg gttggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     2821 aaagaactgc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     2881 gtggatacgt tagccggcct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     2941 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3001 caggtatgga atttcgccga ttttgcgacc tcgtaaggca tattgcgcgt tggcggtaac
     3061 aattgaccta catgggcctc gggctttgct acagcctggt gaatattcct tatggttcac
     3121 ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg tctgggcgcg gctcgtggga
     3181 ttgccgcttc attgaccttt gtatgcctgg catttctgat aggaccgagc attaagaact
     3241 ccagcccgga agagatggtg tcggtatacc atttctggac gattgtgctg gtgattgccg
     3301 gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga aaatgtggta cgtatcgtgg
     3361 cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg gaatcgcccg ctgtttatgt
     3421 tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc ggtcagcgcc tcgtcgttgt
     3481 tctacgtgcg ctatgtgtta aatgataccg ggctgttcac tgtgctggta ctggtgcaaa
     3541 acctggttgg tactgtggca tcggcaccgc ttgtgccggg gatggtcgcg aggatcggta
     3601 aaaagaatac cttcctgatt ggcgctttgc tgggaacctg cggttatctg ctattcttct
     3661 gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt ggccatcgct tcaattggtc
     3721 agggcgttac tatgactgtg atgtgggcac tggaagctga taccgtagaa tacggtgaat
     3781 acctgaccgg cgtgcgaatt gaagggctca cctattcact attctcattt acccgtaaat
     3841 gcggtcaggc aatcggaggt tcaattcctg cctttatttt gggattaagc ggatatatcg
     3901 ccaatcaggt gtaaacgccg gaagttatta tgggcatccg cacatcaatt gccttagtac
     3961 cttgcggatt tatgctactg gcattcgtta ttatctggtt ttatccgctc acggataaaa
     4021 aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa agtgcagcag caattaatca
     4081 gcgatatcac taattaatat tcaataaaaa taatcagaac atcaaaggtg caactatgag
     4141 aaaaatagtg gccatggccg ttatttgcct gacggctgcc tctggcctta cctctgctta
     4201 cgcggcgcaa ctggctgacg atgaagcggg actacgcatc agactgaaaa acgaattgcg
     4261 cagggctgat aagcccagtg ctggcgcggg aagagatatt tacgcatggg tacagggagg
     4321 attgctcgat ttcaatagtg gttattattc caatattgtt ggcgttgaag gcggagcgta
     4381 ttatgtttat aaattaggtg ctcgtgctga tatgagtacc cggtggtatc ttgatggtga
     4441 taaaagtttt ggctttgccc tgggggcagt aaaaataaaa cccagtgaaa atagcctgct
     4501 taaattaggt cgcttcggga cggattatag ttatggtagc ttaccttatc gtattccgtt
     4561 aatggttggc agttcgcaac gtacattacc gacagtttct gaaggagcat taggttattg
     4621 ggctttaacg ccaaatattg atctgtgggg aatgtggcgt tcacgagtat ttttatggac
     4681 tgattcaaca accggtattc gtgatgaagg ggtgtataac agccagacgg gaaaatacga
     4741 taaacatcgc gcacgttctt ttttagccgc cagttggcat gatgatacca gtcgctattc
     4801 tctgggggca tcggtacaga aagatgtttc caatcagata caaagtattc tcgagaaaag
     4861 cataccgctc gacccgaatt atacgttgaa aggggagttg ctcggctttt acgcgcagct
     4921 cgaaggttta agtcgtaata ccagccagcc caatgaaacg gcgttggtta gtggacaatt
     4981 gacctggaat gcgccgtggg gaagcgtatt tggcagtggt ggttatttgc gccatgcaat
     5041 gaatggtgcc gtggtggata ccgacattgg ctatcccttt tcattaagtc ttgatcgtaa
     5101 ccgtgaagga atgcagtcct ggcaattagg tgccaactac cgtgtaactc cgcaatttac
     5161 gctgacattt gcaccgattg tgactcgcgg ctatgaatcc agtaaacgag atgtgcggat
     5221 tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc agcgaagggc cgttacaagg
     5281 gatgaatttc tttcttgctg ccgataaagg gcgggaaaag cgcgatggca gtacgctggg
     5341 cgatcgcctg aattactggg atgtgaaaat gagtattcag tatgacttta tgctgaagta
     5401 agaaataacg ccggagagaa aaacctccgg cgtttcagat tgttgacaaa gtgcgcgttt
     5461 tttatgccgg atgcggcgta aacgccttat ccagcctaca aaaactcata aattcaaagt
     5521 gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag gcaatctctg gtttgttttc
     5581 agatgaaaac gccggagtga aaattctccg gcgttttggc cgtgaattac tgctgcggaa
     5641 ttgccggtac agccggaacg ttaagagctg gcatcgcaaa catgccaaca aaatcttcta
     5701 acgacatttt ctgcccattt aacgttatct gaccgttagc atattgcagg ctggtggtga
     5761 tggtattgtc ctgcaaggtg gtcagacgga acatctgccc cattgctgat gcaccttcaa
     5821 cttgctgttt cgccagtttt ttcgcttgat cttcctgata accttccagc ttcgctacct
     5881 gagtcatcaa ctcagttgcc atatccaccg gaatggtcag tttcgcatcc agagatttaa
     5941 ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc tttagtcgtt gccggatctt
     6001 tcaggaacag cgacagattc agcgcacttt caccctgact gtttttccag cttagcggcg
     6061 cgatagtaat caccggatcg cctttcaaca tcagcggcag ggcgctaaag aaggcttccg
     6121 tcactttctc ctgataaagt tcgggattgt tggcaatttc tggctgtgcc agcagcgcct
     6181 gagtttgcgc gttatattgc tggctaaact gatgccaggc ttcaccatca atctggccga
     6241 cttttaaagt cagcttgccg ctgcccagat cctgattctg taccttcagg ctgtttagcg
     6301 agtaatccag ttggctattg atcgttttac cgtcattgac cagatccgat ttaccgctga
     6361 tctccatgcc ttccagcagt gccagttctt tgccttccac tgaaatggtc attttttcca
     6421 gtgacagttt ttggtttcct acgcgctcac caaaacttcc cagcgtgctg gaaccgtcgg
     6481 ttttcagatt attaaaggtc aactgcactt tctggttgta ttcgttaact gcgtctatcc
     6541 gaccactttg cgcctctccg gaaagggaga tggctttgcc gtctctgtca gcatttaact
     6601 ggaactcgcc gccgctaaag gcgacttttt catccttttg ctcgtaattc agtggattga
     6661 gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg cgagttaatc tcaaaaggcg
     6721 tttcaccttt tgccatatca aacagtggtt tgcttacttc gttattaacc agcgtggttt
     6781 gaattgatgc catcgacggg atcaggttca gttttttaag ctgggcaagc gggaagggac
     6841 catgatcaac cgattcgttg aagatgacgc tctgaccgct tttaatccac ggattttctt
     6901 tcccggcaat gggtttcacc aacagttgca actggctgct gaatacgccg cgatgatagt
     6961 tttgataact cacttccagg ttggattcag gagctgtcag tttgagttgc gcgttcgcct
     7021 gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc tgtataccat gcgccgcctg
     7081 tccagactac gcctagcgca acaatgacgc ctaccgctac cagcgattta ttcataatga
     7141 ttatccataa aatgaaatca ggcggactgg ccgcctgaag gtgttataag cctttaataa
     7201 gcttagcaag agatgttaat tttttcagta agctcttaca gcttgttgta aacacgcgct
     7261 aaacggccgt ggcctttgac agtcaccggt gattcgttgg cggcaataaa cgctgattca
     7321 cccggtttaa gctgtaactg ctgagaacct ttccacaacg ttgcatcgcc ttcgacgcag
     7381 aacaaaatgg cggcactctg ctggctgatg gtggtttctt tatcactaag gtcatgcagc
     7441 gagaaggcaa aatcatccac aggaatcggg aagtccagtt ctgcaccttg tttcaccggc
     7501 tgggtcaaca actggttagc cggtttggct tcgaatttca cattggcaac cagttccgga
     7561 atatcaatgt atttaggcgt cagacctgca cgcagcacgt tatcggagtt cgccatcact
     7621 tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag cgaacaggaa catcgcttcg
     7681 ccaggattca atttcaccac attcagtaac agcggggaga acaggccgct gtcttccggg
     7741 taaaattcag aaattaaacg aatcgtttgc cacggttcac cctgctgact gtcgagcgcc
     7801 gattttaaaa tcgccagcgc gcgggatttt tcttcaccct gcatattcaa caggttggcg
     7861 aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa agtgagcaat cgccggatgt
     7921 gcaccggcga ccggctggag tagggagaca atctcggaaa attcacgaaa cgcgttcatc
     7981 gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt ggttaggatc tttatagtta
     8041 cgctcggcgg catccatcgg gatacctgcg gcattttctt tggcaaaacc gatttcagaa
     8101 ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg ctgcgcataa tactttgaac
     8161 aggaaaggca gttcgccaaa gcgtttggca acggcctctc cgagcagagt cgatttatca
     8221 ctctcaatca catcacgcag tgaaacgata tctccggcgg cattctgcac tcgtgaactg
     8281 cttttcggat gtgcgcccat ccacagctcg gccatcggct ggctggacgg attttccata
     8341 ccataaagtt cagtcaacgc cgttttgcta ccccaggcat agttttgcac tgagttaatg
     8401 agtttttgca tgatcaatcc ctgttttaat gtggaaatta atcccgctat taaagcaaga
     8461 atcctacggg aagtaacctg gagccgcaaa aagtcgtact agtctcagtt tttgttaaaa
     8521 aactgtgtag gatattgtta ctcgctttta acagggcaac ggaacacccg cccagagcat
     8581 aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa accctttcat tatcagtctc
     8641 cttttccact caaaaaaaat gatactgagt attacctgct aaccagtgaa cacgttagcg
     8701 tatctgaatt tgaagggcag gagattttga aagtcgcacc cgaagcgtta actctgttgg
     8761 cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc ggctcaccaa caacaggtgg
     8821 ccgacattct gcgtgacccg gaggccagcg aaaatgataa atatgtggcg ctgcaattcc
     8881 tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac ctgtcaggat accggcaccg
     8941 cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg tggtggtgat gaagcggcgc
     9001 tggcgcgcgg tgtctataac acttatatcg aagataatct gcgctactcg caaaacgcgc
     9061 cgctggatat gtataaagaa gtgaataccg gcaccaatct gccagcgcag atcgatcttt
     9121 atgccgttga tggcgacgaa tacaaattcc tctgtatcgc aaaaggtggt ggttcggcaa
     9181 acaagacgta tctctatcag gaaaccaaag cgttactgac gccggggaaa ctgaaaaatt
     9241 acctggttga gaagatgcgc acgctgggta ccgcggcctg tcctccgtat catattgcgt
     9301 tcgttattgg tggaacttct gcagaaacga accttaaaac ggtgaaactg gcttccgcga
     9361 aatactatga tgaactgcca acggaaggga atgagcacgg tcaggcgttc cgcgatgtgg
     9421 aactggaaaa agaattactg atcgaagcgc aaaatctcgg tctgggtgcg cagtttggtg
     9481 gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc acgtcacggc gcatcctgtc
     9541 cggttggtat gggtgtttcc tgctctgctg accgtaatat caaagcgaag atcaaccgtc
     9601 aggggatctg gatcgaaaaa ctggaacata atccaggtaa atatatccct gaagagctgc
     9661 gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa ccgtccgatg aaagagatcc
     9721 tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc gcttaacggc acgattatcg
     9781 ttggtcgtga tattgctcac gccaaactga aagagcggat ggataacggt gaagggctgc
     9841 cgcaatacat taaagatcat ccgatttact acgcgggtcc ggccaaaacg ccggaaggtt
     9901 atgcctccgg ttctcttggc ccaacgaccg ccggacggat ggattcttat gtcgatcaac
     9961 tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg caaccgcagc cagcaggtga
    10021 cggatgcctg taaaaaacac ggcggcttct accttggcag tatcggtggt ccggccgctg
    10081 tattggcgca gggaagtatt aagagcctgg aatgtgttga atatccggaa ctgggaatgg
    10141 aagccatctg gaaaattgaa gtggaagatt tcccggcgtt tatccttgtg gatgataaag
    10201 gaaatgactt cttccagcag atacaactca cacagtgcac tcgctgtgtg aaataaacag
    10261 agccgccctt cggggcggtt tttttacatg gcacgaaaga ccaaacattt gttatcaaat
    10321 agtaaataat gagtgagcta aaagttgctt aacgaaagca aaacagaaag aaaaaattaa
    10381 tcaggtgagg agcaggccat gaatacagta cgcagcgaaa aagattcgat gggggcgatt
    10441 gatgtcccgg cagataagct gtggggcgca caaactcaac gctcgctgga gcatttccgc
    10501 atttcgacgg agaaaatgcc cacctcactg attcatgcgc tggcgctaac caagcgcgca
    10561 gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag agaaagcgag cgccattcgg
    10621 caggcggcgg atgaagtact ggcaggacag catgacgacg aattcccgct ggctatctgg
    10681 cagaccggct ccggcacgca aagtaacatg aacatgaacg aagtgctggc taaccgggcc
    10741 agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag ttcaccctaa cgacgacgtg
    10801 aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga tgcacgttgc ggcgctgctg
    10861 gcactgcgca agcaactcat tccgcagctt aaaaccctga cacagacgct gagtgaaaaa
    10921 tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc acttgcagga cgccacgccg
    10981 ctaacactag ggcaggagat ttccggctgg gtagcgatgc tcgagcataa tctcaaacat
    11041 atcgaataca gcctgcctca cgtagcggaa ctggctcttg gcggtacagc ggtgggtact
    11101 ggactaaata cccatccgga gtatgcgcgt cgcgtagcag atgaactggc agtcattacc
    11161 tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc ttgcgacctg tgatgccctg
    11221 gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac tgatgaaaat cgccaatgat
    11281 gtccgctggc tggcctctgg cccgcgctgc ggaattggtg aaatctcaat cccggaaaat
    11341 gagccgggca gctcaatcat gccggggaaa gtgaacccaa cccagtgtga agcattaacc
    11401 atgctctgct gtcaggtaat ggggaacgac gtggcgatca acatgggtgg cgcttccggt
    11461 aactttgaac tgaacgtctt ccgtccgatg gtgatccata atttcctgca atcggtgcgc
    11521 ttgctggcag atggcatgga aagttttaac aaacactgcg cagtgggtat tgaaccgaat
    11581 cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc tggtgactgc gcttaacacc
    11641 cacattggtt atgacaaagc cgcggagatc gccaaaaaag cgcataaaga agggctgacc
    11701 ttaaaagctg cggcccttgc gctggggtat cttagcgaag ccgagtttga cagctgggta
    11761 cggccagaac agatggtcgg cagtatgaaa gccgggcgtt aatctgcaac atacaggtgc
    11821 agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt tataacggtg ctgcacattg
    11881 tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc ccgcgccatt atcccgatta
    11941 atcagtgcaa tcagtggtgt agggcaggcg tgttggactt gtttttgatc tcctttgtac
    12001 cagacgcggg caatcggctg caccttcact ggacgtttga tttttaactt cgcgttctgt
    12061 ggcagggcag cgatatcctg atactctcgc tccagttttc tttgccactc ctcgcgcgtc
    12121 cacggtgcga cactgcgtgg tgatttcagg cttttttcca gctgtgccag gacttcatca
    12181 cgatgcaaat tcttaatgat atgtttatta gcccaaccaa agcgtaaagt ggcggggtcg
    12241 tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca gccccggcaa atgacgatgc
    12301 acccattcaa aacgtgccgc ggtggggagt tctgattcaa ccgtgacgat atgctcgaac
    12361 gtggtcttga gtttattgat gtgctgaata tgactgacca acgctgcttg cgaaaggtta
    12421 tcgacctggt aacacaacac gccaggcaga cggacagcgg ccttgctgct gcgattttcg
    12481 gactgttgtt gaataaataa atggcggaaa tgacgcaacg ccagcgactg cgcgtcgttg
    12541 ccgagatgtt gttttacctc aatacgatta agcggattat gctcatcctc tttttttacc
    12601 tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt gctcaagatg agcggcaaat
    12661 gtagccagct cttgttccat ctggcgaaag gtagtgttga gtcggtctac gagatcgtaa
    12721 cgcgccataa tattaaccac tttagttaca acatacttat tttatagcac agtcgtggtg
    12781 gcttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt gcgggatgtt atgccataac
    12841 ggccagctaa acgagaagcg agcaccaccc agttcgctga tgtcacaatt aaccgtaccg
    12901 cccattgcca gtgctattga gtggacaatt gccagcccca gcccgcagcc gccggttgag
    12961 cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga tatgttcgcg gttttctggg
    13021 gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc tattccccga cagtagcagg
    13081 ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat tgagcaaatt atccagcacg
    13141 cgctccatta agcgcatatc caacgccgca taatggcctt gcatgagcgt ttttatccgt
    13201 accgttttat cgggcgttac tgcctgaata tctgccagat gcgttgacag ccacaacggc
    13261 aggtctggtt cgctaagatg aagctcgttt tgtgggcgat cgagtcgggc ataagtcagc
    13321 agctcttcaa ttaaagcttc aagttgactg atatcacgat tcaacgcctg ggattcggcg
    13381 gcgctcaggt tatcgctcat ctccagtcga taacgcaggc gcactaacgg tgttcgcagt
    13441 tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa ttaaggcgtt gatattgtcc
    13501 gccatctggt taaatgcgat accaagtcgt tcaaagctcg aaccctcatc aaagtggata
    13561 cgttcattga gatgcccatc gccaaatcgt tgcgccgctg cttccagttt taacatatcc
    13621 tgccagtgcg gacgcatcca gataaacacc ggaaaggcga gggaaatagc aataaaagcg
    13681 atcagggcga tatccagcaa tcgcatttga tggaggtaat aaagataagg aacaggacca
    13741 actgccagca cgtagtggct gcgcgggata cgctgcaaaa acgtgtactg atcgtccagg
    13801 gcgacaattt cgccgccacg cagtcggtgc atggaaatat catcaagatg gtatttactc
    13861 agtggctcga cacgcagatc gaaagagaga tttaaatcca tctctttcag cgtcttaccc
    13921 cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca gatacagcga actgttcatc
    13981 aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa atttgtacac cagcccaacc
    14041 agcagagaca tcacaaggaa gcagacaaac aataacaggt aaaactggat aaacaatttt
    14101 ttcattggca tcgcccgcaa cagaatacta ttcgtattaa ttcacgactg tggagcaatg
    14161 taaaccgcca actggataag accttgtttg gaagataatg tccggaacag acggatgtat
    14221 ccccggtgta ttcatattca gtaaattcaa ttttacaccg gggcttgttt attatgctaa
    14281 ggtttaattt aacgtgacgc tttgttgttc tttagtctct ctatccttaa gaagacgggc
    14341 tgatattaca ccggacatta ttgaaccatt cacattaagc gctgttcttg ccatatcaac
    14401 gatcggctct atggcaataa aaataactac taatgcgaca gggaaattaa gtgttgaaag
    14461 gacaatcagc gccgcgaatg tcccaccact acctacgcct gctaccccta ttgagccaag
    14521 cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag aagggatcga tacccattgt
    14581 tggagcaatc atcgccacca tcacggcagg ataaatccca gcgcagccat tttgtcccat
    14641 gctggaacca aaagacgctg caatattagc aatcgtattt ggaaccccca atttttccaa
    14701 tgcagaaata gccagaggaa ttgaagccgc actactgcga gaaacaaacg caaaggtaag
    14761 tacttgccag acggtcttgt aatagttaac ggggttttta cctattaaca gcaaaatgcc
    14821 cgagtgaaca ataaacatca caaaaacggc cagataacag gcgacgatga acccgagcaa
    14881 attggtaaac tgtgaaagct gatacgttga gaagactttc gccattagtg ccattacccc
    14941 ataaggcgtc agcgcaatga caatacggac cattttcatt acccatattt gaatcgcgtt
    15001 gatccccgcg ctcaattttt cgccctctgc tggtgcatct ttctttactt ttaacagcgc
    15061 aataccagca agaattgtaa agatgacgat gccaataacc gaaacacttc ttgccccggt
    15121 cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg agttgcggta aagataccgc
    15181 agctgttttc gagacgtcat cgctggttaa agaggattgc atatgaccaa aggcgctggc
    15241 atcaaggcca attgcactgg caatagccag gccaactaaa cctgcaatca tgactaaaca
    15301 caacatacag gcaacaatag ttaaagacat cttgccaata ccagcgctat tttcgagctt
    15361 attaatcgct gaaagaatcg aaatgaatat gagtggaacg gcaacaagtt ttaataaatt
    15421 aacataaccg ttaccagcaa tattatacca ttcgataacg gtttcactgc ttgtctcagg
    15481 aatgaaatgc agggagatac caatgatagc ccccaggaaa atcgcgataa aaacactttt
    15541 ggaaagggat tcgctctttt gatccgtttt gaaaatcccc catgagcaga tcaaaaaaag
    15601 cacaatatat gtgaaagttg taaccgtttt ttaagcctct tgcctcattc agcacctgaa
    15661 atgccggaaa gagtttgttg taaaaaatct gcgccataag acgcagggac gaggcgatta
    15721 tagagaaatt ttttctcatt ttcatatcaa atgtaggggg tttggaattt gaataagctc
    15781 ttattaattg aattcgttaa ttgttctctt tttcagatga acaaatatta gtcatctgat
    15841 tgtttattgt cactatccca tgcatgaggc gcaaaaagat agcctttgtt acgcacagtt
    15901 ttaatgcgat aaggttctgc ggcgttatcg agcagttttt ttcttaaccg cgaaatagcc
    15961 acgtccacgc tacgatccag tccgtcataa ctgacgccgc gtaaattttt cagcaatgca
    16021 tcgcggtcca tgatttgccc ggcatgcgta gctagttccc acaataattc gaaatcagct
    16081 gtcgagagcg agatttcagt gttagccagg gtgactacgc ggttgatggg gtcgatagtt
    16141 aacgtgccga aatgcagggc tttgtaggga gtaagagacg tttcctgaag acctttggtc
    16201 agtgtggctt gctcattctg acgcaaatgc aaacgtaaac gcgctagcaa aacagcaggg
    16261 ggcgtcgttt tgagaatata gtcgcaggca cccatttcca gtgccaggat gtggttcata
    16321 tcgctatcga gagaggttag aagaacaatc ggtccagacc actttgcgcg taaatcacga
    16381 caaatggtca tgccgtcctt gcctggtagc atgatgtcga gtaacaccaa atccggattt
    16441 tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt ctacggtaac ctgcatatca
    16501 tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg catcatcttc cacaaatacg
    16561 atagtgttca taacattcac ggtagatata aaaacgtcac aatacaccgc gctgttttta
    16621 cttactatca atcgtttcta aacaagtttt catcaggtat tctactgatg acttgttatg
    16681 gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc gcgctcggtg cgctggtagt
    16741 gctgttgatt ggtgttttag caaaaacgaa aaattattat atcgccgggc tgattccact
    16801 tttcccgacc tttgcgctta tcgcgcatta tattgttgcc agcgaacgcg gcattgaagc
    16861 cttacgcgca accatcattt ttagtatgtg gtcgattatt ccctattttg tttacctggt
    16921 gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc gcttttgttg gttctgttgc
    16981 gtgttggggg ataagcgcat gggtgttgat tatatgctgg ataaagctgc attaacgcag
    17041 atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca aagcaactgg taagtaagta
    17101 atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt ttcatcagtg atttattcag
    17161 cgcctgttgt cgatattcgg catcatcatg ttcattaaac aggatcagcg atggcgcaat
    17221 agaattcact ttcacttccg gtgccagctt gcgggcaaac gagcgggtca tattatccag
    17281 tgccgctttg cttgcagcat acgcaacatg tttgtcgcta ccgcgctcca ccacataatc
    17341 ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca cgcagtaatc tttccagcgc
    17401 atggttgagc aggtatgggg tattaacgtg gatctgcatc atgcaagcca gtacgtcggc
    17461 cagtggcgca cccggttttt ccgcaatcca cgcactggcg ttatgcaaaa tagcacgcag
    17521 accatgggtg gtttttagta cttcatcggc aaacgccatc acaccgtcgt tggtcgaaaa
    17581 atcagcctga atacactgcg cacctgcatt aatcagtcca tcaatggctg gatagtgtgt
    17641 ccgatagctg acaatcaccg gttgcttttg attaatgaaa tgccatgcga gggcgaggcc
    17701 gatgcgacga cctccgccag taattaatat tggcaagggc tgggttttac ccatcgttat
    17761 ctcctttgct atccaacgat gggatgaacg ttatcccacc agcatccagg tcgccggaac
    17821 tgaggcaatt aacagcatgc caatcagcac catttcctga cggttcagta cattatcatg
    17881 tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc ggtgcataca gaacaacgga
    17941 taacagcaag tgcattggac cagaggcgta caataaccat aagccataaa tgcaggcacc
    18001 gacacctacc gctttatgca gtggacgggt tgcgattttc agcaagaacg cgccgaccag
    18061 gaaatagggc accagaatca tttctgaggc gatggtcagc aacgtgttgt aatcggaacc
    18121 ggtcagccag atgagtacca gacaaatttg cacgcagatg ttggtcaacc acagtgaggc
    18181 ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt gggaatgctt tatgagtggc
    18241 agccaggaac ggaacttctg ccgccatgat ggtccagctt aagtacgcgc cgcaaacgga
    18301 aacgatcaaa ccggcagcga tgatgatttc gccccatggt cccatcattt ccaccatcag
    18361 accggccatt gacgggttac gaatttcagc cagttcagga cgtgccacca cacccagcga
    18421 aagcagcgtt accagcaagt aaacgcccag agcggagaga accgccagca gtgtcgcttt
    18481 gccaacatca cgtttattac gcgcacgcgc agaaacaacc acggcacctt ccacaccaat
    18541 gaaaacccac agggtgatca gcatggtgtt tttcacctgt tcccaaacag gtacgccaag
    18601 tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg aacatcatca tcgccagcac
    18661 aacaaacaga cccagcggca acaattttgc cagtgtcgcc accaggttaa tgctggcagc
    18721 ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac aataaagccg atgcaccgac
    18781 tatcgattgc caggtattac catcgccaaa caggcgcaat tccggcgtgt ccgtaaaaaa
    18841 gcttaacgcg gaaaaaacga tcaccagata ggagacgttg gcgatgaccg cgcacagcca
    18901 gtatccccat gcggaacaaa agccgattag ctcgccaaac ccttcgcggg cataggtaaa
    18961 gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc agcatggcaa aggccagcaa
    19021 taaaatgcca gcgccagtaa taccccagcc gatgagcagt gctgccgggc tggcaactgc
    19081 cgccatattt tgcggcagac tgaaaacacc cgcgcccagc attgagctta ataccagcgc
    19141 ggtgagtgcg ctcagtccca gtttcttttc catcgatatc cagtgattaa aacgtgataa
    19201 gaagaataaa tattagggca aaacgcataa aaatggagtt tgccactgaa gcgcgggatt
    19261 ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa tttgtgacta aaaatgcgaa
    19321 aggcggcatc aagccgcctt ataggagtaa ctgaatagtt atttatacag atctgcgctg
    19381 acggtcaggt tattaccacg tttctgccac tggcgggtga tgtggtaata cttagcacct
    19441 ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg tcatgttgcc gtagttgcca
    19501 gagaatttga tgctgtcgaa cgggaccatc atcgctgctg tcgctttgtt cagttcttcg
    19561 actttagtgc catccgggag cgtgacggtg taacgcccgc cttttgatga ctgggtttca
    19621 aagaagcgac cgacttcaga acttggtgat gcggtagtcg caacacccgg gatctcaact
    19681 ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc ctgcttcgga atctgccggg
    19741 atcacatccg ggctctggac gatacgtttc ttagcatctt ttttatagat gaatgcagta
    19801 atacgctggt tgccgccctg gttggcatcg atttgacgaa cgatgtagaa agagtaggca
    19861 cctttcgctt ttgccgcttt ggtgatggca tcattgactt ctggctggct acgatagaag
    19921 ccctggacgg tgaccgtgtc aaacggttca atcagaacag cctgatcttt cggcagttcg
    19981 acaacaccgt taattacgcg attacttgtt tcttctgctt tttcagcatc ggctttatag
    20041 aggtcagcga ccacacgcca gttaccgctg ttaccaaaat cagaagtgtc gacaacataa
    20101 aaagaggcgg caccttcttt atctgcgcga cgagaaacgg ctttcaccgc ttcgccaata
    20161 gcattaaaac gaccggtaac cactacacgg tcaaaaggtt taaccgctgc cgcttgctcc
    20221 ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag cagaaagcag tgccgacgcc
    20281 aggagggtgt tcttaagctt cataaaaata atccttcgcc ttgcgcaaac caggtactgg
    20341 tattgttatt aacgagaaac gtggctgatt attgcattta aacggtgtaa ctgtctgcgt
    20401 catttttcat atcacattcc ttaaaccaat tttaatcctg ctcaaatgac cgtctatgct
    20461 taaaaaacag ccgtatcagc atcattacta ctgaagcaac tgaattgtat aagttaattt
    20521 aatgttaagt agtgattcgt gccggggcga tgtctcgttt tacccgaccg tcgaagacaa
    20581 ttatcagtct ttatccggcg ttctaaggtg tttatcccac tatcacggct gaatcgttaa
    20641 tattttgcga gttcacgccg aaatactgat ttttggcgct agatcacagg cataattttc
    20701 agtacgttat agggcgtttg ttactaattt attttaacgg agtaacattt agctcgtaca
    20761 tgagcagctt gtgtggctcc tgacacaggc aaaccatcat caataaaacc gatggaaggg
    20821 aatatcatgc gaattggcat accaagagaa cggttaacca atgaaacccg tgttgcagca
    20881 acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc
    20941 gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta
    21001 gaagggaata gcgtctggca gtcagagatc attctgaagg tcaatgcgcc gttagatgat
    21061 gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt ttatctggcc tgcgcagaat
    21121 ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg tgatggcgat ggactctgtg
    21181 ccgcgtatct cacgcgcaca atcgctggac gcactaagct cgatggcgaa tatcgccggt
    21241 tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct tctttaccgg gcaaatcact
    21301 gcggccggga aagtgccacc ggcaaaagtg atggtgattg gtgccggtgt tgcaggtttg
    21361 gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc gtgcattcga cacccgcccg
    21421 gaagtgaaag aacaagttca aagtatgggc gcggaattcc tcgagctgga ttttaaagag
    21481 gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg acgcgttcat caaagcggaa
    21541 atggaactct ttgccgccca ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt
    21601 ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg ttgactccat gaaggcgggc
    21661 agtgtgattg tcgacctggc agcccaaaac ggtggcaact gtgaatacat cgtgccgggt
    21721 gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt ataccgatct tccgggccgt
    21781 ctgccgacgc aatcctcaca gctttacggt actaacctcg ttaatctgct gaaactgttg
    21841 tgcaaagaga aagacggcaa tatcactgtt gattttgatg atgtggtgat tcgcggcgtg
    21901 accgtgatcc gtgcgggcga aattacctgg ccggcaccgc cgattcaggt atcagctcag
    21961 ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca
    22021 ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc tgttcggttg gctgtcaagc
    22081 gttgcgccga aagagtttct tgggcacttc accgttttcg cgctggcctg cgttgtcggt
    22141 tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac cgttgatgtc ggttaccaac
    22201 gcgatttcag ggattattgt tgtcggagca ctgttgcaga ttggccaggg aggctgggtt
    22261 agcttcctta gttttatcgc ggtgcttata gccagcatta atattttcgg tggcttcacc
    22321 gtgactcagc gcatgctgaa aatgttccgc aaaaattaag gggtaacata tgtctggagg
    22381 attagttaca gctgcataca ttgttgccgc gatcctgttt atcttcagtc tggccggtct
    22441 ttcgaaacat gaaacgtctc gccagggtaa caacttcggt atcgccggga tggcgattgc
    22501 gttaatcgca accatttttg gaccggatac gggtaatgtt ggctggatct tgctggcgat
    22561 ggtcattggt ggggcaattg gtatccggct ggcgaagaaa gttgaaatga ccgaaatgcc
    22621 agaactggtg gcgatcctgc acagcttcgt gggtctggcg gcagtgctgg ttggctttaa
    22681 cagttatctg catcatgacg cgggaatggc accgattctg gtcaatattc acctgacgga
    22741 agtgttcctc ggtatcttca tcggggcggt aacgttcacg ggctcggtgg tggcgttcgg
    22801 caaactgtgt ggcaagattt cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa
    22861 cctggcggct ctggtcgttt ccttcttgct gctgattgta tttgttcgca cggacagcgt
    22921 cggcctgcaa gtgctggcat tgctgataat gaccgccatt gcgctggtat tcggctggca
    22981 tttagtcgcc tccatcggtg gtgcagatat gccggtggtg gtgtcgatgc tgaactcgta
    23041 ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc aacgacctgc tgattgtgac
    23101 cggtgcgctg gtcggttctt cgggggctat cctttcttac attatgtgta aggcgatgaa
    23161 ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc gacggctctt ctactggcga
    23221 tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa gagacagcgg aactgctgaa
    23281 aaactcccat tcagtgatca ttactccggg gtacggcatg gcagtcgcgc aggcgcaata
    23341 tcctgtcgct gaaattaccg agaaactccg cgctcgtggt attaatgtgc gtttcggtat
    23401 ccacccggtt gcggggcgtt tgcctggaca tatgaacgta ttgctggctg aagcaaaagt
    23461 accgtatgac atcgtgctgg aaatggacga gatcaacgat gactttgctg ataccgatac
    23521 cgtactggtg attggtgcta acgatacggt taacccggcg gcgcaggatg atccgaagag
    23581 tccgattgct ggtatgcctg tgctggaagt gtggaaagcg cagaacgtga ttgtctttaa
    23641 acgttcgatg aacactggct atgctggtgt gcaaaacccg ctgttcttca aggaaaacac
    23701 ccacatgctg tttggtgacg ccaaagccag cgtggatgca atcctgaaag ctctgtaacc
    23761 ctgacggcct ctgcggaggc cgtcactctt tattgagatc gcttaacaga acggcgatgc
    23821 tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat aattgtcaac ggcacggaaa
    23881 gcagcatacc caccggtcct aacaaccatc cccaaaaaat caacgacaaa aataccacca
    23941 atgtcgaaag ccccagccca cgtcccatga tgcgcggctc cagaatattg ccgaagacca
    24001 gattaatcag cagatatccc gccagcacca gcaacgcttc gtagaagcca ttaaacacca
    24061 gtacctgagc gatagggggg attgccgcga gaactgaacc aatattaggg atgtaattaa
    24121 gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg
    24181 cccaggcgac caggccggtg atgatgctga tggctgtttt cagcaccaga taatgagaaa
    24241 cactgtcaat cgcacgttga atcgccgcca tcccttcaac cggacgcgcc atcatttgct
    24301 gaaattttcc gggcaattgt ggcacttcga gcagcataaa cagcaccgtc agcagcaata
    24361 aaaatattga tgacatggca ttagataact gcgtcaataa gttggtgagc aacgtcatcg
    24421 ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga gacgtcaatc cctacgcgtt
    24481 gcaacaacgg ctcaagagct tgcagcggcg tcataataga gttgcgatat tgcggtaacg
    24541 tccacgtcaa ctcgttgagc gcggaaccca gataagctaa tagcaacacc atcgccatca
    24601 cgatgattgt catcaaaatc gacactgcca gtacacgtgg cacacgccag cggaccatat
    24661 gttgcaccag cgggttaaga ataacagcaa taaataatgc gagaataaac ggcacgatga
    24721 tctcggcggc aaaacggata ccgcagagaa taatgaccag cattcccaac ataatgacga
    24781 tttttaggcc attgagcgtg atgatcggct ttgccatgct tgtccccgtt tttcttcctt
    24841 tgctaataat aatgtctttt tcgctccgtc atcataacga aaatcaaacc gctcaggtaa
    24901 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    24961 accgaggaca attatcatct gcgatgacga gaagcaacac tgcggataat tgtaatatta
    25021 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    25081 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    25141 ttgccctatg cgcagggctt gaccacttta ctttggtttc gctgaattaa gcgaaaatta
    25201 aaatagttct cttgcaggag aaggacaatg tatatttatt ggattttatt aggtctggct
    25261 attgctacag aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    25321 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    25381 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtatcgg tattttattt
    25441 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    25501 ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag gtacccgtaa agcgcgtaaa
    25561 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    25621 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    25681 caaaatattt ggcttgctct ccctggcggc ggtgctggcg gcctttagtg cgctttctca
    25741 ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg tggggcgggt ttggtattgc
    25801 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    25861 tggcctggtc ttgctgttgg ctggaatgat catggtgaaa cttgcctgat gaagacgctg
    25921 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    25981 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    26041 cgaactttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    26101 gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc gacgtttgcg cccagccagt
    26161 cacttcacag ttttgatgac tgtataacgt atcgagatga tcttcagggt atcccgcctg
    26221 agtcacttta cgaccagccg attttaatgc ggcagtaagc gcggctttat ctccctcaaa
    26281 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaatc cgaagtccca
    26341 cggtgcagct gcgggaggaa caatccaacc atccccatct gcttttaacc gctttcccag
    26401 tgtcggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    26461 cacaaaacgc agcgctaccg ccttatccgc tttaccctta ggcggcgtca ataaacagtg
    26521 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    26581 cgtttccagt tgcccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    26641 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    26701 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    26761 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    26821 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacagtc aggaaagata
    26881 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    26941 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    27001 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    27061 gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg cgtgtttctt cgctgcctgc
    27121 gctttttgtt caggggcttt ctgttcagct ttcgtatttt tatgatgctt tttagccgcc
    27181 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtagt tttcgctggc
    27241 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggcg
    27301 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    27361 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    27421 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    27481 ataaattaca gcgttgataa taacgtattg tgacttaagg aaaatttagc taccaataat
    27541 agtagtcttg atcgggttaa ctattatact acttacaaag cggtagaata actgcgcatc
    27601 agtaataaat gacacacagc aaaatgaatc cgtttatttg ggtacttata atcctgatga
    27661 cgctagatgc gctgcgggaa ttggctggcg cttcgtctat tttaggatgg ctattaacgt
    27721 tggtttgagc aggcaataag tccggacggg tatttaccgc agtccggact tatttttcag
    27781 gcgtgcagac gacgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    27841 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    27901 gcaatactcg accccagata gtaactgaac agatacagcg aggaggccta gcctttagcg
    27961 cgttttgcgc gggggccgat ccagctgctg gctactgagt gggctgcgaa gaatccggct
    28021 gagaagagta acattccggc aaagatcagc cacagcgagc taaataaggt catcagtaaa
    28081 ccaaacggca taactcccgt cgaaaacaac atcaccggac cgcgcccata gcgggtggtc
    28141 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    28201 accacggcct gactgacatg ctagggggaa agcatcaacc gatagccgat gtaattaaac
    28261 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    28321 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcaa
    28381 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    28441 ccgattgccg ccagagcaat tcgccagttg aaaaagtccg tgaagacacc actaattaag
    28501 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccatcga aaaggccacg
    28561 aaactggggt ggatttcctc gctaagataa gtcatgccaa cagctgccac gccacttaac
    28621 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    28681 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    28741 gcatcggata gcgggccagt aaatagcaaa ccgatagcca acatcgccgt ggaaatggac
    28801 agtgaaatac tactgttcgc gggcgttaag ccaaactcct gcgaaagcac cggaaggata
    28861 ggctgcacac aatagagaag tgcaaaggtt gccagtccgg cagagaacag cgccagggtg
    28921 acgcgcataa attgcggcgt accgcgttta ataaattgat ttggctgaga aatgctttgc
    28981 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    29041 tgctaaatat gcctgtggat caggcttata catagggtag gaaaatcgaa ttgttctgtc
    29101 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    29161 cgttactttg ttgctgttgc ggaagagctg cattttgggc gcgcggctgc ccgcctgaat
    29221 atttcgcaac cgccgctaag tcagcagatt caggcactgg agcaacaaat cggtgcccga
    29281 ctgctggcac gaactaatcg cagtgtattg ctgacggcag caggaaaaca gtttcttgca
    29341 gatagtcggc aaatcctgtc tatggtggat gacgctgccg ctcgcgctga aagactgcat
    29401 cagggtgaag cgggggagtt gcgtattggt tttacttcgt ccgctccttt tattcgggcg
    29461 gtgtccgata cgttatcgct gtttcgccgt gattatcctg atgtccattt acaaacccgc
    29521 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    29581 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    29641 atggcgatga tcccgtacga tcatcccctg gcaaataatc cgagtgtaac actggctgaa
    29701 ctggctgaac tggcgaaaga accctttgtc ttttttgatc cgcacgtcgg gactgggctg
    29761 tatgacgata ttctcggact gatgcgacgt taccatttga cgcccgtcat cactcaggag
    29821 gtgggcgagg caatgaccat catcggtctg gtttccgccg gtctgggtgt ttcaattttg
    29881 cctgcgtcat ttaaacgtgt tcagctcaac gaaatgcgct gggtgccgat tgctgaagag
    29941 gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag tccagctgcg
    30001 cgtaactttc gtattcatct gctgaatgct ctcaggtgag ggaaatttca gcgaaaaagc
    30061 ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt tgacgacacg tattgaagtg
    30121 cttcaccata gcctacagat tatttcggag cgcgaaaata tagggagtat gcggtggttg
    30181 ctgaaaacca gcctgggcac attgatcaaa taaagcagac caacgcgggc gcggtttatc
    30241 gcctgattga tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg gcgcagctgg
    30301 ctcctgccag tatcactaaa attgtccgtg agatgctcga agcacacctg gtgcgagagc
    30361 tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt ggggctggtg gttgaaactg
    30421 aagcctggca ctatctttct ctgcgcatta gtcgcgggga gattttcctt gctctgcgcg
    30481 atctgagcag caaactggtg gtggaagagt cgcaggaact ggcgttaaaa gatgacttgc
    30541 cattgctgga tcgtatcatt tcccatatcg atcagttttt tatccgccac cagaaaaaac
    30601 ttgagcgtct aacttcgatt gccataaccc tgccgggaat tattgatacg gaaaatggta
    30661 ttgtacatcg catgccgttc tacgaggatg taaaagagat gccgctcggc gaggcgctgg
    30721 agcagcatac cggcgtaccg gtttatattc agcatgatat cagcgcatgg acgatggcag
    30781 aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt atcgatcaca
    30841 acgtgggggt gggcgtcatt accgatggtc atctgctaca cgcaggcagc agtagtctcg
    30901 tggaaatagg ccacacacag gtcgacccat atggtaaacg ctgttattgc gggaaccacg
    30961 gctgcctcga aaccatcgcc agtgtggaca gtattcttga gctggcacag ctgcgtctca
    31021 atcaatccat gagctcgatg ttacatgggc aaccgttaac cgtggactca ttgtgtcagg
    31081 cggcattgcg cggcgatcta ctggcaaaag acatcattat cggggtgggc gcgcatgtag
    31141 ggcgcattct tgccatcatg gtgaatttat ttaacccaca aaaaatactg attggctcac
    31201 cgttaagtaa agcggcagat atcctcttcc cggtcatctc agacagcatc cgtcagcagg
    31261 cccttcctgc gtatagtcag cacattagcg ttgagagtac tcagttttct aaccagggca
    31321 cgatggcagg cgctgcactg gtaaaagacg cgatgtataa cggttctttg ttgattcgtc
    31381 tgttgcaggg ttaacatttt ttaactgttc taccaaaatt tgcgctatct caatttgggc
    31441 caggaaagca taacttagac tttcaaggtt aattattttc ctagtttata tttgtgaagc
    31501 ataacggtgg agttagtgat gctgaagcgt ttctttatta ccggtacaga cacttctgta
    31561 gggaaaacgg tggtttcccg cgcattgcta caagcgttag cctcccaggg aaaaagggtt
    31621 gcgggatata aacccgtagc gaaggggagc aaagagacac ccgaagggct gcgtaataaa
    31681 gatgccttgg tgttgcagag tgtttcaacc atcgaactgc cttatgaagc agttaatcct
    31741 atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa ttacaccctc
    31801 atttcaaacg gcctggcaaa cctgaccgat aaagtcgatc atgtcgtggt agaagggact
    31861 ggcggctggc gcagtctgat gaatgatttg cgtccactct ctgaatgggt agtgcaggaa
    31921 caactgccgg tgttgatggt tgtcggtatt caggaaggtt gcattaacca tgcactgcta
    31981 acagctcagg cgatcgccaa cgacgggctg ccgctcattg gctgggtggc taaccgaatc
    32041 aacccaggac tggcgcatta tgcggaaatc attgatgtgc tgggtaaaaa acttccggca
    32101 ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac agcgtgaact ggggcaatac
    32161 atccgcttag ctatgctgcg cagtgtgctg gcggtagata gagtcacggt gtaacgtccg
    32221 cgaaattacc gacgcaatta cgcaggcaat caataaaccg gggagtagct gatactcccc
    32281 ggtcatttca catatcatca acgtcgacat aatcggcgcg tgcgtggttg ccgccaacag
    32341 tgtcgccatc ccggtcaatc cgagtaaaag cgtaatttct tcgccatcag ggaaccataa
    32401 tcccaggcta cgaccataca acatgccaat ggcaagaccg ataaatagcg tcggtgtaaa
    32461 gacaccaccg ggtgcgccgg aaccgctact cgccagcacg gcacacagtt tacaaaggaa
    32521 gatcccggca atgatcatta acagtggtgg ggcggttaaa aaggattgta cggtgctata
    32581 gccgttgccc cacactgcag gtgtgaacag ggaaagcaga cccacgatca acccgactag
    32641 tgccagttgc cagggcggcg caagtttgag actcacaaat ccacgatgac aggcgttcat
    32701 taacgttaac aacagtggtc cgcacagacc tgccagcaca cctgtactga taatcagcgc
    32761 atagtcacga gcctgaaccg tcactgagag ttgtacgttg tagagtaacg cgtcgctatg
    32821 attaatcaga ttgctaacca gccatgccac gatggcggaa ataatcaccg ggccgagaga
    32881 ggccaacatc atagtgccaa acagcacttc ggctataaat aaactgccag caagcggggc
    32941 acgataggcc gcagccattc ccgccgcggc cccacaggcg atccataatt tccattcctg
    33001 gcgtggcgta aaacgttggg caaaacagga ggcggcaagg gcagctaaaa gaatcatcgc
    33061 accttcgcga ccaattgcac tgccgctggt tactaccagc agcgaggcaa gcgatttaac
    33121 caggcttgct gcgtaatcga actgtccatc ggtttgcaac gcttccatgt aatcggtcgg
    33181 cgcatgaggg cgttgttggg taaatttctg ccagcccatc agcaacaaac ccgccgccag
    33241 tccgccgagc gccggagtta gcagccgtcg ccaggaggaa aggtttgtcg ctgcattgac
    33301 cagactgccg gagtcattat tgaggaacaa ccactccagt agcagcatcg catgacgaaa
    33361 cccggcaacg gcaaagacag cgagaatacc gacgactgtt gcgataagca gacggcggaa
    33421 catagtgcgc agatccggat agatgtgtag atgatgcatg agcagaaatg ggcaatgaaa
    33481 cacgggcgac tattttgcgg caaaagaggc caaccgcaaa agcagtgcgt gctttaagag
    33541 ataaaaaaac gtgacattgt aacgtattat tgccgaatcg cgcgtaatcg tcaccatccg
    33601 gcaatattac ggtgatccta tcgaaagagt ggtttaaccg cgacaggaat caacagttgt
    33661 gattgccatt gcgccagcgt taagcgagcc agttcaccca gtgcacggta gaaagggtgt
    33721 tctgcttttt cgataaaaac atcaagaaaa cgtgttgacc acggaaaaag gtgccatgcc
    33781 agcaattctt cgcattctgt ctggcgacca ttctccgcca accacgcagc catcaacagc
    33841 agtgaaccaa aatgatcttc cggttcgttt tgcttcattt caaactgaat gcctttctcg
    33901 cgcatccact gacgaagtgc caatgttgaa tcgccaaaca gcacagattc gcgatccagc
    33961 cagaccgacc cccacggcgg agacggcagt gcccacgggc cgacaaacaa acgctgccag
    34021 gcctgggcgt gagtctcttc acactgtgtc tgaaaagccg tcaccagcgg cgctaatgac
    34081 gcttctggta gaggccactg agtttcccag ccgtcactgg tgagtaccgc aaccagaggt
    34141 gcggcttccg cactctctgg agcgtaataa aacagcgcgc ccaacacgcg cgccgcgaca
    34201 gaaaaattat cttgctgtga aaaatgggtc attcctaaca tcctgaaatg tgcgggttgc
    34261 cccgcaccaa tttgttaacc tgcaatagcc attcctactg tcatgtgcag gccataaaag
    34321 agcacacgcc cgatcatctc accaacaagt atgaggatta gccccagaac aagtccggca
    34381 acgtgaggtt cacggcgacg aatgagcgga caaagccagc atccaagacc tgcgcataac
    34441 aataccacac gccagacctg taaggaggcg taatctggta ccagtgcgct ggcttgttgc
    34501 acggatgagt gaatagacgc cagagacaaa ccttgcagca caatcacctc ggcacaagcg
    34561 atcagcgcca gaacgctaat gatggcaaat ggtgtggtat taaatgtaac gcgggctgcc
    34621 cgcagaattg cggcagcaag tattggaccg ctcaacaata ccgtcaggaa gaaagccagc
    34681 gtagtgtaac cgttatgcca ggttggcacg gtgtcgattt gatacacgca ggtcatcatc
    34741 cagacgaaaa tgacgccgag cgccatgctg acaagtaacc agagtttccc caacgcttgc
    34801 ggcattttac cgatgaccgc caccagccac catagtccgc caacggcaaa aaatatggaa
    34861 ccggcagcaa tctcgttact cagaccagaa gctccgattc gattcagcga gttaaacgct
    34921 cgcagtggcg atcccaggtg catgacagag gcaataaacc cgatgcccat caacagccag
    34981 aggaaaaaca tgccacgaac aatgtgctga cggtctgcgt catttttggc ggcgaaccag
    35041 ccaattccgc taacaatcag cgcgcctact acgcattgac ccaatacggt aaagataacc
    35101 agtggccatt catgccatcc atttcccatt ttacacttcc tccggattag ccagataacc
    35161 agtggtatcg ccggtcgggc ggctgttggc gttaggtttg ataacgatat tgggttttgt
    35221 gaaatgcgcg cgcggcagtg gcgcgacggc agccagagtg ccgtgcttct ggcgcagctc
    35281 ttcaattgga ccgaactcaa gtgcacgcag cgggcaggat tcgacgcata tgggttgttt
    35341 cccctcggcg acgcgcgaat aacaaccatc gcacttcgtc atgtgccctt tttcagcatt
    35401 gtactgtggt gcgccgtacg gacaagccat gtggcagtag cggcagccga tacaaacatc
    35461 ttcatcaacc accacaaagc catcttcacg cttatgcatt gcgccgctgg ggcagacttt
    35521 tgtacatgcg gggtcatcgc aatggttaca ggagatggag agataataag cgaacacgtt
    35581 ctggtgccag atgccattat cttcctgcca gtcgccgcca gcatattcat aaatacggcg
    35641 gaaactgact tccgggccaa gatctttaaa atctttgcac gccagttcgc aggttttaca
    35701 gccagtacag cggctggaat cgataaaaaa tccatattgt gtggtcatgg gctactcctt
    35761 aaaccttttc gatctggaca agattgctgt gcgacgggtt tccctttgcc agcggtgaag
    35821 ggcggtgaga ggtcagaata ttgatactgc cgccgtgatc gacccggtca ccaaacatat
    35881 ccgctttaag ccacgcacct tgcccgatgg cggtaacgcc aggcagaata cgcggagtca
    35941 cttttgcggc aatcagcatt tctccattat tgttaaatac ccgcacggta tcgccatgac
    36001 ggataccgcg tgcctgagca tcaatggggt tgatccacac ctcttgtggg caggcctgct
    36061 gtaacacatc aatattgccg tagctggagt gggtacgcgc tttggtttgg cgacttcact
    36121 cagcatccag tagatgggtt tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat
    36181 cacatagccc atattccctg cagattcatg agagataagg tcttcttgct ctgttggcat
    36241 caggtcaggc aacaggatat cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc
    36301 aacaatcatc tcgcacttgc tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc
    36361 atgttgattg atcaatgtgt tactggcgta gcaccataaa aacttgatgg ggacatccag
    36421 tttttctttt ccacgaacac catcacgggt cgcggtcatt ttcgtaccat ggtcgatggc
    36481 atctgtccat gtaaaaacgg aaatctgcgt tttaacagga ttctcgagca tcgggaacca
    36541 ttctaccccc agatcccagc taccttcgcg tacacctgag ttgccgccgt ttatgccgac
    36601 gttaccggta agaacggaaa gcatggcaat agcgcgggac gtttgttcgc cgttggagtg
    36661 tcgttgtggc ccccaaccct gacaaatata agcaggtttt gctgaaccga tctctcgtgc
    36721 caactggata attttttctg ccgggatgct ggtgattttt gctgcccatt ccggcgtttt
    36781 agctatgccg tcaggccctt cgcccagaat ataggcttta taatgcgcgt tacgtggtgc
    36841 gttggcgggc agcgtttttt catcgtaacc aacacaatat ttgtcgagaa atggctgatc
    36901 gaccatgttt tcagtaatca gtacccaggc aatcgcacag gccagtgcgc catcggtgcc
    36961 agggcgaatg ggcagccatt catcttcacg cccggcagct gtgtcgttat agcgtggatc
    37021 gatgacgatc atgcgtgcgt ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc
    37081 accgccgctc atccgcgttt ctgccgggtg atttccgaac ataacgacca gtttcgtatt
    37141 ggcgatatca tccgggctgt tgccatcatt ggcaccgaac atataactca ttgcggcact
    37201 gatctgtgcg gtactgtagc tgccatagcg actgagaaaa cccccgcaag agttcatcag
    37261 acggtacggg acgttcgagt tggtgatatt tccgccatct acgcctgttc cgtacaggac
    37321 atgtacagcc tcattgccgt aatctttcag gatccgccga agattatcac tgatggtatc
    37381 cagggcttcg tcccaactta tccgttcaaa tttaccttca ccgcgctcgc cgacgcgctt
    37441 catgggatat ttcaacctgt caggatgatt catccgtcgg cggatagagc gcccgcgtaa
    37501 acatgcccga acctgatgat taccgtaaac gtcgtcacct gtcgtatcag actccaccca
    37561 gtacacggtg tcatctttca catgcaaacg tagcagacag cggctcccgc agttaacggt
    37621 gcaggaactc cagaccgttt tctcttctac cggagcctct gccgcccgga ccatttggga
    37681 aaatggcaga gtgaaagcac tgcttgccag cgcgagactg ccaagtgcgg aggttttcat
    37741 cagacttcta tggctgattt cagccttcat gagcgcctct gtggtatgga ttttcatcat
    37801 tactcactta ttgcttttca aacaaaatgt catgccagaa tttatggttg tagtgggtta
    37861 tattttttcg atctcgacca gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg
    37921 gcgcagagtg gttagcgtat tcacacatcc gccatggtcg attttatcgc cagacatatt
    37981 gggctcgtgc caggctccct ggcccatagc gctaactcca gggagaatgc gtggtgttac
    38041 tttggctggt agccgaactt cgccacgatg gttaaacacc cgcaccatat cgccgttggc
    38101 aatcccacgt ttctgtgcat ctatagggtt gatccacacc tcctgacggc aggctgcctt
    38161 caggacatca atgttgccgt aggtcgagtg agtacgggat ttgtaatgga aaccaaacag
    38221 ttgcagtggg aaggttctac gttcagggga gtcccagcct tcaaaggttg aggcataaac
    38281 tggcagtggg cttatcactt catctttttc cagttcccag gtacgggcaa tttccgccag
    38341 cttgctggaa taaatttcaa tcttaccgga aggcgtttta agtggatttg cctcggggtc
    38401 gtcacgaaat gctttgtagg cgacaaaatg gccattggga tctttacgct tatagatacc
    38461 catttttttc agttcgtcgt aagacggtaa cgccggatct ttggcaagca ttttggcgta
    38521 cagatgttgt aaccattgtt cctgcgtgcg accttctgtg aacttttgat agacgtcagg
    38581 tccaagacgt ttcgcgactt cactcaggat ccagtaaatc ggtttacgtt cgaatttttc
    38641 gctggtgact ggctggagga aaatgagata tcccatgtta ccggcgtagt cgttaggaat
    38701 aatatcttcc tgctcaacgg tcatcaggtc tgccagcaga atgtcggcat attttgccga
    38761 tgaggtcata aagttttcga tgaccacaat catttcgcat ttcgattcgt cctgcagaat
    38821 ttcatgcgtt ttgttgatgt cagaatgctg attaacgagg gtatttcccg cgtagttcca
    38881 gatggactct actgtcagac ggatggacgg acttgaagta gttgactcaa cgctacttaa
    38941 ggtcctgata tgactgacat taaccatgac gatactgcat atccatccag ttctttctct
    39001 gggtctccct atctgtatca gcaagtctac acctcaagtc ctgacacaag gctgacacaa
    39061 atcaaggacc ttgctacgcc acagctagtc cggagaacca acgggcgtta cactgtccgc
    39121 ttcagactta aaggagctac cagcccgttt ctatcagtct caacccgcag cacagaccgg
    39181 aggaccgcta caatgcgtca gagagagctc gcagccacag ctaaggcatt cctactggac
    39241 aatcctgaag tcacactaca ggagcttaca gcgcacatac ggtccatggc tgagcagttc
    39301 ctgactgatg ccagtgatga ctactggaat ggcgtagagg tggcgacact ggttgacgag
    39361 aagtccaacc tcagggaact tgcagcgaca agagcgttga gtgtggacca gcagaggggc
    39421 attaagttag cgctggaagt cctgaccgca gcacagcaga gagtcgacac tggtgatacc
    39481 tctggtctga ttaagctgat tgatggtggc gtaagccagc ttactgatga taataaccat
    39541 actgattact caactatagg cgactctacg tcgattctga agaatgagca gggggataga
    39601 ccagcagttt tcacacagga acgtcagtta ctcactccag agtcacgaga atcatctgta
    39661 ctttttcagg aattggtgac atcactgctg gctgagaagg tccagacact gaaacaatcc
    39721 agctttaagg acttgtcagc gtccc
//
LOCUS       DADLCQ010000024        39552 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000024 DADLCQ010000000
VERSION     DADLCQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.024"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1156)
                     /gene="setB"
                     /locus_tag="JQG95_001113"
     CDS             complement(<1..1156)
                     /gene="setB"
                     /locus_tag="JQG95_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY5887747.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLF"
     gene            1524..2654
                     /locus_tag="JQG95_001114"
     CDS             1524..2654
                     /locus_tag="JQG95_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY5887748.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2654..3592
                     /gene="fruK"
                     /locus_tag="JQG95_001115"
     CDS             2654..3592
                     /gene="fruK"
                     /locus_tag="JQG95_001115"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY5887749.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3609..5300
                     /gene="fruA"
                     /locus_tag="JQG95_001116"
     CDS             3609..5300
                     /gene="fruA"
                     /locus_tag="JQG95_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY5887750.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5724..6665
                     /locus_tag="JQG95_001117"
     CDS             5724..6665
                     /locus_tag="JQG95_001117"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY5887751.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSARKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENTECLN"
     gene            6653..7591
                     /locus_tag="JQG95_001118"
     CDS             6653..7591
                     /locus_tag="JQG95_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY5887752.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7685..8935
                     /locus_tag="JQG95_001119"
     CDS             7685..8935
                     /locus_tag="JQG95_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY5887753.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="JQG95_001120"
                     /pseudo
     CDS             complement(9006..9700)
                     /gene="yeiL"
                     /locus_tag="JQG95_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9831..10772
                     /gene="rihB"
                     /locus_tag="JQG95_001121"
                     /pseudo
     CDS             9831..10772
                     /gene="rihB"
                     /locus_tag="JQG95_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
     gene            10872..12122
                     /locus_tag="JQG95_001122"
     CDS             10872..12122
                     /locus_tag="JQG95_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY5887754.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12178..13266)
                     /locus_tag="JQG95_001123"
     CDS             complement(12178..13266)
                     /locus_tag="JQG95_001123"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY5887755.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="JQG95_001124"
     CDS             complement(13269..14126)
                     /gene="nfo"
                     /locus_tag="JQG95_001124"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY5887756.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLHTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14200..15249)
                     /locus_tag="JQG95_001125"
     CDS             complement(14200..15249)
                     /locus_tag="JQG95_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY5887757.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15348..16229
                     /locus_tag="JQG95_001126"
     CDS             15348..16229
                     /locus_tag="JQG95_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5887758.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DEQVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16435..17904
                     /gene="lysP"
                     /locus_tag="JQG95_001127"
     CDS             16435..17904
                     /gene="lysP"
                     /locus_tag="JQG95_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY5887759.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18196..20187
                     /gene="cirA"
                     /locus_tag="JQG95_001128"
     CDS             18196..20187
                     /gene="cirA"
                     /locus_tag="JQG95_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY5887760.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYMVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="JQG95_001129"
                     /pseudo
     CDS             complement(20219..21054)
                     /gene="yeiG"
                     /locus_tag="JQG95_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21312..21980
                     /gene="folE"
                     /locus_tag="JQG95_001130"
     CDS             21312..21980
                     /gene="folE"
                     /locus_tag="JQG95_001130"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY5887761.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21997..23073
                     /locus_tag="JQG95_001131"
     CDS             21997..23073
                     /locus_tag="JQG95_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY5887762.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRLWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23215..24255
                     /gene="galS"
                     /locus_tag="JQG95_001132"
     CDS             23215..24255
                     /gene="galS"
                     /locus_tag="JQG95_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY5887763.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24535..25533
                     /gene="mglB"
                     /locus_tag="JQG95_001133"
     CDS             24535..25533
                     /gene="mglB"
                     /locus_tag="JQG95_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY5887764.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25594..27114
                     /gene="mglA"
                     /locus_tag="JQG95_001134"
     CDS             25594..27114
                     /gene="mglA"
                     /locus_tag="JQG95_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY5887765.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27130..28140
                     /gene="mglC"
                     /locus_tag="JQG95_001135"
     CDS             27130..28140
                     /gene="mglC"
                     /locus_tag="JQG95_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY5887766.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28176..29389)
                     /gene="preA"
                     /locus_tag="JQG95_001136"
                     /pseudo
     CDS             complement(28176..29389)
                     /gene="preA"
                     /locus_tag="JQG95_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29383..30621)
                     /locus_tag="JQG95_001137"
     CDS             complement(29383..30621)
                     /locus_tag="JQG95_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY5887767.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30815..31054)
                     /locus_tag="JQG95_001138"
     CDS             complement(30815..31054)
                     /locus_tag="JQG95_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY5887768.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="JQG95_001139"
     CDS             complement(31057..31776)
                     /gene="sanA"
                     /locus_tag="JQG95_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY5887769.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="JQG95_001140"
     CDS             complement(31926..32810)
                     /gene="cdd"
                     /locus_tag="JQG95_001140"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY5887770.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32940..33635)
                     /locus_tag="JQG95_001141"
     CDS             complement(32940..33635)
                     /locus_tag="JQG95_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY5887771.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33632..34030)
                     /locus_tag="JQG95_001142"
     CDS             complement(33632..34030)
                     /locus_tag="JQG95_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY5887772.1"
                     /translation="MSKTLNIIWQYLRAFILIYACLYAGIFIASLLPVTIPGSIIGMQ
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34269..35216
                     /gene="dusC"
                     /locus_tag="JQG95_001143"
     CDS             34269..35216
                     /gene="dusC"
                     /locus_tag="JQG95_001143"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY5887773.1"
                     /translation="MRVLLAPMEGVLDSQVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISDC
                     DAVMIGRGAFNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35634..37487)
                     /gene="asmA"
                     /locus_tag="JQG95_001144"
     CDS             complement(35634..37487)
                     /gene="asmA"
                     /locus_tag="JQG95_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY5887774.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRRNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(37509..38090)
                     /gene="dcd"
                     /locus_tag="JQG95_001145"
     CDS             complement(37509..38090)
                     /gene="dcd"
                     /locus_tag="JQG95_001145"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY5887775.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(38182..38823)
                     /gene="udk"
                     /locus_tag="JQG95_001146"
     CDS             complement(38182..38823)
                     /gene="udk"
                     /locus_tag="JQG95_001146"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY5887776.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            39141..>39552
                     /locus_tag="JQG95_001147"
     CDS             39141..>39552
                     /locus_tag="JQG95_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887777.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         9657 a         9798 c        10370 g         9727 t
ORIGIN      
        1 aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga taattccaga
       61 tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc gacgtgttgg
      121 tatagagcgt ggtggctgaa cctgcctgac cgggcatcaa atcctgaaaa tagagcatcc
      181 cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc aacagaatgg
      241 caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg gcagcaacgc
      301 gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac atcgtcggga
      361 tttccagccc ggcggcggtc cccatcatca caccggctag tttctcggga agatgcagtt
      421 cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc cacatcaatg
      481 tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggcgct tcaaccgtgc
      541 cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc atcacgccgc
      601 aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa cccatcgcta
      661 acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg cgtaaaaaag
      721 agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca agggcaaaca
      781 tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat agcaaaacaa
      841 agtagttgcg attccaagca aaaagggtgc aggccagcac gcctaacagg cagcaaaaga
      901 caatcagcga tttgcgatcg ccgcgcttat cagagcgccc agcgagaaac tgacttacca
      961 gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga cgggcatgta
     1021 cttcatcggt aaggaaaata ctgagtgtcg gggtttgcag agcacccgca ataccggtga
     1081 gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg ctggagactg
     1141 cgggggagtt atgcatgctg gtcgatcact ggaagaggaa gacgcggagt ttacgctgct
     1201 tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa atgccatttc
     1261 actgttgctg attggctgct gacgctgctg aaatgaaatt gctgataacg gattttccca
     1321 tcagcaatta ggaaaaatga caaaaaattg tgcagcacat caaacttttg ctcataactt
     1381 tacggctttc cttgcgtgct gaaacagaag tattatgctt tcttgaaacg tttcagcgcg
     1441 atcttgtctt taaccctaag ctaggttggc gctttttttc tcatagaggc tgaatcgttt
     1501 caattcagca agagaggaga actatgttcc agttatccgt acaggacatc catccgggcg
     1561 aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg gtgcaggccg
     1621 gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa acctcaacgt
     1681 tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat caggtgctga
     1741 aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac ggtcaggtag
     1801 cgtacgtggc aatcggtatt gctgccagct cggataaaca tctgggcctg ctacgccagc
     1861 tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca acaacagcag
     1921 aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag ctcgacaacg
     1981 aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg ctgaacgctg
     2041 cgcgtctgaa agaggcgagg gcagttgacg ccactttcgt caccaaagcc atcaatgaac
     2101 aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc aatctgcgta
     2161 gtgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa acggcagcca
     2221 tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag cgtctcgctg
     2281 atttgttgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca acgttgctgg
     2341 cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag tttgtagtgc
     2401 gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc attaaacaat
     2461 ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca aacggacgta
     2521 gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc tttaccgccc
     2581 agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct gctggtcttg
     2641 gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg cttatgacct
     2701 tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa ccaccggtct
     2761 gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg gaattgatgt
     2821 caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac tgttcagcga
     2881 gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta acgttaagct
     2941 gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggtttcgaag tcacccccgc
     3001 cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg atatggtctg
     3061 tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact ggatgactcg
     3121 cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt tagtagcagg
     3181 tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa tctgggcagg
     3241 ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac gtgaacaagg
     3301 tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta atgcctccgg
     3361 cgaatggatc gctaaaccac cgtcagtcga tgtcgtaagc accgttggcg caggggattc
     3421 tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac acacactgcg
     3481 tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta ttaccgatcg
     3541 tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact gacagcagga
     3601 gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca cgcgcctata
     3661 tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc atcgacaatc
     3721 cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac agcgcgctga
     3781 acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct gagctgttcc
     3841 tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcactgcg acagcaccgg
     3901 ttgccgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact ggcgtagcac
     3961 acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc tggtgggtga
     4021 aagttgaaac ccgtggttct gttggcgcgg gtaatgcaat cactcccgag gaagtagccg
     4081 cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa tttgctggta
     4141 aaccgatgta tcgtacctct accggtctgg cgctgaagaa aacctcgcag gaactggata
     4201 aagcggttgc tgaagcaacg ccgtatgaac cggcgggcaa agctcaaacg gcgaccactg
     4261 aaggtaagaa agagagtgca ggcgcttatc gtcacttgct aacgggcgtc tcttacatgc
     4321 tgccgatggt cgttgctggt ggtctgtgta tcgcgctttc ttttgctttt ggtatcgaag
     4381 cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggtggt ggttcagcct
     4441 ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc gatcgtccgg
     4501 gcctcacgcc gggtctgatt ggcggtatgc tggcggtcag caccggttct ggcttcattg
     4561 gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt acgcaactga
     4621 aactgccgca gagtatggag gcgctgaaac cgatcctgat cattccgcta atttccagtc
     4681 tggtggtcgg tctggcgatg atctacctga tcggtaaacc agttgctggc attctcgaag
     4741 ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg ggggcgatcc
     4801 tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg tacgcattcg
     4861 gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg gcggcaggta
     4921 tggtgccacc gctggcaatg ggcctggcaa caatggtggc gcgtcgcaaa ttcgacaaag
     4981 cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt tcggaaggtg
     5041 caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc gtgggtgggg
     5101 cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcaccg cacggtggtc
     5161 tgtttgttct gctgatccct ggcgctatta cgccggtatt gggttacctg gtagcaatta
     5221 ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg gaagtggacg
     5281 cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc tgccctgtaa
     5341 catttgatga gcgcaaagag aatgaccagc ttgtgaatgt aattttatga atttaaatgg
     5401 ttttatcttt cagtagaaac ttcaaacgtt catcaaacac taaggttatt tcctcaaggg
     5461 cagattattt tctgcccttt tttagtgttc ctgttctgtg catcccgtca caaattcctc
     5521 gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct gcccattttg
     5581 cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca tgtttaaata
     5641 tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga cagggttggt
     5701 aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt ataggttcgg
     5761 cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat tcaaatccag
     5821 gtaaaataaa atttacgcct ggtggagtag ggcgcaatat tgcacaaaac ctggcgttgc
     5881 tgggtaacaa agcctggcta ctgagcgccg taggcagtga tttttatggt caatcgctgc
     5941 taacgcaaac caatcaatct ggtgtttatg tcgataaatg cctgattgtg ccgggagaaa
     6001 atacgtcgag ttatttatca ttactcgata ataccggtga aatgctggtt gctataaatg
     6061 acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt gaatttattc
     6121 agagggcaaa ggtcattgtc gcggactgta atatcagtga agaggcactg gcatggattc
     6181 tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgcgg aaatgtgtca
     6241 aagtgcgcga ccgtctaaat cagatccaca ctctgaagcc aaaccgcctt gaagcggaaa
     6301 ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct gcctggttcc
     6361 atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt tattacagcg
     6421 atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt aatgttaccg
     6481 gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga atgccgtttg
     6541 ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt gaatacacca
     6601 ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacaca gaatgtctga
     6661 attaaaaatc tcccctgaat tattacaaat ttctccggaa gtgcaggacg ctttaaaaaa
     6721 caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc cgttcccaca
     6781 aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg cagtacctgc
     6841 cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaaa aaattgaatt
     6901 actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac cttttgttgt
     6961 tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg cggcgcttgc
     7021 cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg cggaacatac
     7081 cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg ttgtttgtgc
     7141 cggggcgaaa tctattctcg atttaggatt aaccactgag tatttagaaa ccttcggtgt
     7201 gccgttaatt ggctatcaga ctaaagcgct gcctgcgttt ttctgccgta ccagctcgtt
     7261 tgacgtcagc attcgtctcg acagcgccag tgaaattgcc cgtgcaatgg cggtgaaatg
     7321 gcaaagcggg ctgaacggtg gcctcgtggt agcgaacccg atcccggaac agtttgcgat
     7381 gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg aagagcaggg
     7441 cgttattggt aaagaaagta caccgttcct gctggcgcgc gttgctgagc tgaccggcgg
     7501 tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg cgagtgaaat
     7561 tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc cgtcgcccgt
     7621 actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg aagggaaaat
     7681 cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa tagcatttct
     7741 gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac tgctgctgca
     7801 aatcgctatt ggtggcatca tgctctactt cccaccggga aaatgggcag tagaacaggc
     7861 ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct tcatttttgg
     7921 ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta tcttcgcctt
     7981 tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt actacattgg
     8041 cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagccc tcaacatcag
     8101 caaaatcgaa tcttttgttg cggttaccac tattttcctc gggcaaaatg agatcccggc
     8161 gatcgttaaa ccgtttatcg atcgcgtgaa tcgcaacgag ttgtttaccg caatttgtag
     8221 cgggatggcg tccattgctg gttcggtgat gattggttat gccggaatgg gcgtaccaat
     8281 tgactacctg ttagcggcat cgctgatggc gatccccggc ggtattttgt ttgcacgtat
     8341 tcttagcccg gcaaccgagc cttcgcaggt cacatttgaa aatctgtcgt tcagcgaaac
     8401 gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc tgaaaatcgc
     8461 cgctggtgta gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga tcaacggcat
     8521 tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa gtatttttgg
     8581 ctatgtgctg gcaccgctgg cgtggatcat gggtgtggac tggagtgatg ccaaccttgc
     8641 tggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc tgagtttctc
     8701 cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta tatcctttgc
     8761 gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat tttcggctat
     8821 ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg cagcagcaac
     8881 gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg cgtaatgctc
     8941 gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc tattgcaaat
     9001 aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag cgccagtcct
     9061 gacaactgct ttctgttttt aattagatat cctttctttt aattaataaa ccgtcgtgaa
     9121 tgaattgcgc gagaacatat aaaagatgtc gataagaaac gcctaaatac tctgccgctt
     9181 gcgtatgctt ttcgtgataa agatcacctt cttgtgagag taatataaat gctgccaggc
     9241 gattcactaa cggaaatgat tgattctgag ttaaagaaac aatattacga taatttttat
     9301 gacttaaggt gacgcaaagt tttcgtaaaa atagggtgtc gtttaataac agcggacggt
     9361 aatgtttcat agggagcgca aggcaccaac actcttcaat agcctgcacc gcacgcggtt
     9421 catggtcttt gtcgattaac tcaatctcgc caataaaaca gggagcggca aagaaatcga
     9481 tcagcgacac gcgaccattg gcaagcgtgg cgtaaagcct ggcgcggcct cgcgtcagat
     9541 aaaacagcca tgacggctgt tgcccttcct gcacaatgta gtcacgcgct aaaaaatgaa
     9601 acagccgcgt atcggctgaa acatccgttg aaaagcaatc cttaaacgca gattcactca
     9661 tcaattgctg cttaaggtca ttattgtgga tttctttcac gatgtttatc cgctatgaga
     9721 tttctcatat ttagcatacg tacgctgttg ttaagattca atcattggcc cggtcgaatt
     9781 ttctttgctg tagaaaaaaa taacgcaact ggaaacagag gaaataaaca atggaaaaga
     9841 gaaaaattat tctggattgt gagccgggtc atgatgatgc tattgcaatg atgatggcag
     9901 cgaaacatcc ggcaatagat ttattaggca tcactattgt agcgggtaat cagacgcttg
     9961 ataaaacatt aattaatggc ctgaatgttt gccagaaact ggagattaat gttccggttt
    10021 atgcggggat gccgcaaccc attatgcgta aacaaatcgt tgccgataat attcacggtg
    10081 aaaccggact ggatggcccg gtattcgagc cgctgacccg ctaggcagaa agtacccatg
    10141 cggtgaaata tatcatcgat accctgatgg caagcgatgg cgatatcact ctggtgccgg
    10201 ttggtccgct ttcaaatatc gcggtggcaa tgcgtatgca acccgcaatc ctgcccaaaa
    10261 tccgtgaaat tgtgctaatg ggcggcgctt acggtacagg caacttcacc ccatctgccg
    10321 agttcaatat ctttgccgac ccggaagccg cccgcgtggt gttcacctcc ggcgttccat
    10381 tagtgatgat gggcctcgat cttaccaacc agaccgtttg caccccggat gtaattgctc
    10441 ggatggaaag ggtaggcggc cctgccggag agctgttcag cgacatcatg aacttcactc
    10501 tcaaaacgca gttcgaatac tacggcctgg ctggcggccc ggtgcatgac gccacctgca
    10561 ttggttatct gatcaaccct gatggcatta aaacccagga tatgtacgtc gaagtggacg
    10621 tcaacagcgg cccttgctac gggcgtacgg tctgcgacga actgggcgtt cttggcaagc
    10681 ccgctaatac caaagtcggc atcactattg ataccgactg gttctggggc ttagtcgaag
    10741 agtgcgtgcg cggctacatc aaaacccatt aagtgttgat aacaagccgg gacagtatcg
    10801 caagatgcag gcccggctat ttctgatggg cagaaagccg tcgcccaaaa tgacatgaag
    10861 agataatcac tatggatgtc atgagaagtg ttctgggaat ggtggtactg ctggcaattg
    10921 cgtttttact gtcagtaaac aagaagaaga tcagcctgcg taccgttggc gcggcgttag
    10981 tgttacaggt cgtgattggc gggattatgc tttggttacc gccagggcgt tgggtcgcag
    11041 aaaaagtcgc ttttggcgtc cacaaagtga tggcgtacag cgacgcaggt agcgcattta
    11101 tcttcggttc tctggtcgga ccgaaaatgg ataccttatt tgatggcgca ggatttatct
    11161 ttggtttcag ggtattaccg gcaattatct tcgtcactgc actggtgagt attctctact
    11221 acatcggtgt gatggggatc ttaattcgca ttctcggcgg tatcttccag aaagctttaa
    11281 atatcagcaa gatcgagtca ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa
    11341 ttccggcgat cgtcaaaccc tttatcgatc gtctgaatcg caatgaatta tttacagcga
    11401 tttgtagtgg catggcctcg attgctggtt cgacaatgat tggttacgcc gcactgggcg
    11461 tacctgtgga atatttgctg gcggcatcgt taatggcgat ccctggcggg atcttgtttg
    11521 cccgcctgtt aagcccggct acggaatctt cgcaggtttc ttttaataac ctctctttca
    11581 ccgaaacacc gccaaaaagc attattgaag ccgatgcgac aggggcaatg accgggctga
    11641 aaatcgccgc cggtgtagcg acagttgtta tggcatttgt cgccatcatt gcgttaatta
    11701 acggtattat cggcggcgtt ggcggctggt ttggttttga acatgcctcg ctggagtcca
    11761 ttttaggtta cctgctggcc ccacttgcgt gggtaatggg tgttgactgg agtgatgcaa
    11821 atcttgccgg gagtttgatt ggacagaaac tggcaataaa tgaatttgtc gcttatctca
    11881 atttctcacc ctatctgcaa acggctggca ctctggatgc taaaaccgtg gcgattattt
    11941 ccttcgcgtt gtgcggtttc gctaactttg gttctatcgg ggtggtggtg ggggcgtttt
    12001 ctgcggttgc gccacaccgt gcgccggaaa tcgcccagct tggtttacgc gcgctggcgg
    12061 cggcgacact ttctaatctg atgagtgcca ccattgccgg gttctttatt ggtttagcgt
    12121 agtgtgcaat ttatgagccg gattagatgc ggtcaagcgt cgaatccggc aacattgtta
    12181 caccattggc actaatgaca gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc
    12241 gctggcgcgt gaaattgccg cgcaggccat cgcaaaacgg gcgctgtcac ggaaattgtt
    12301 tccctccaga aagctatata ccagacccgc cataaaacca tcgtcagcac caaaactgtc
    12361 taccgtcgta tgcgctggca cagtcagcaa aaattgttca ccatcctttt cgctgcaata
    12421 aactgactca tcgggcaaat aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt
    12481 cactgcggca ttacggtcag catcgctggt gatcgcctgt ccccataaaa tttccagctc
    12541 cggtaaagtg ggtttcaggg tgtgaatatg cgccagccag tgtttgattt tgcccgcttt
    12601 gaattctgaa acggtatcga caaacaccgg gatctcatca gcgagggtaa agacccattc
    12661 cagcgcctcg gctgtcaggt tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc
    12721 gcgcgacccg ttcagtagtt gcggtgtcaa ctgttccagc agatgggtat cgttaattgc
    12781 cagcacggtt tgatcgtctc gattggcaat tgccagatac gtcgatgtgc tttgaccatg
    12841 caaacgaacg cagccggaga cattcacgcc ggcacggcgc gtttcttcca ggagcatttc
    12901 gccataaaag tcatcgccaa tcactgaaag caaatggacg tcacggccta acagcgccag
    12961 attgtgggcg atattgcgtc caacgccgcc tgccgaacaa tgaattgtac cgggatgaga
    13021 agccgcttgc gggtaatgga tatccgccat cccgcgaata tccatattga ttgtccccac
    13081 cactacgcag tattcctgct cggtgagaat gtaacctttg tctttaatcc ggcctttgcg
    13141 cattaaatcc ataatatgcg ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat
    13201 ttcgttctgc tgaatcagcg ggttacgccg taaaattgca aggatctcct tttcccggtt
    13261 attcatcttc aggctaccgc tttttcagtt tgttgcgctt tcagccaggc gatctcttct
    13321 gcccagatat ccgggttgat ggtttcaagg atcagcggaa tgccgtcgaa acggtcgttc
    13381 tgcataatcc agcggaacgc atcatgaccg atattgcctt caccgaggct atgatggcgg
    13441 tcaacgcggc tgccaaaggt acttttcgca tcgttaaggt gcatcccgcg cagatactta
    13501 aagccgacaa tacgggcaaa atcagcgaac gttttttcgc attcggctgg agtatgcaaa
    13561 tcatacccgg cagcgaaagc atggcaggta tcaatgcaga cgccgacgcg ggatttatct
    13621 tccacgccgt cgataatcgc cgcgagatgt tcgaatttaa accctaagtt actgccctga
    13681 ccggcggtgt tttcaatcac cgctgttaca ccttgagttt tatccagcgc aatgttgatg
    13741 gattcggcaa tacgcgcaag gcaatcctct tctgaaatct gcatcagatg gctgccaggg
    13801 tggaagttga gcaaagaaag ccccagttgt tcgcaacgct gcatttcatc tataaaggca
    13861 tcgcgcgatt tttccagagc ttcagtgacc ggatgtccga ggttgatcag ataactgtcg
    13921 tggggaagaa tttgcgccga tgtgtagtga tatttttcac aggcggcttt gaattcatcg
    13981 atggtttgcg tcgtgagcgg tgcggcacgc cactgacgtt ggtttttggt gaacaaggca
    14041 aacgcggttg cgtcgatttc ggcggcgcga attgcggcat ttgccagacc gccagcagca
    14101 ctaacgtgcg ctccaatgta tttcatgcga ggactcctgt taaacccgct ggaggaaaac
    14161 ggtaatgata gcgggttaac aggagcaaga agtagtggtt tatgcgatga cgctttgaat
    14221 cacatagtta atcgcaccac caccaacaat cagccaggca aacagtacca gtgccatcag
    14281 cagaggtttc gccccagctt ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc
    14341 catcgccatt gccagcagga aggtatccag cgttaccagc atgttcacca cgctctgcgg
    14401 taacaggtgg aacgagttaa agatggcaac tacgatgaac aagatggcaa accacggaat
    14461 agtgattttg cttttctcgc cgctgttcgc cccagacagc tgtttaacac gcgccgccag
    14521 caggatgagg aacggagcca gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt
    14581 ttctgcatcc gggctgatgg catgacctgc cgccaccacc tgcgccactt cgtgcacagt
    14641 agaaccaatg tagataccga aagtttccgg actaaaccat tgagacatca gcggatatat
    14701 cgccgggtag aggaaaatcg cgacggtccc gaagataaca acggttgcaa cagccacggt
    14761 tactttactg gcttccgctt tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca
    14821 gatactgcta ccggcaccga tcaaccaact ggtgtgctta tccagaccaa acactttctg
    14881 ccccaggaag caagccagca ggaaggtact ggacagcgtc agcacgtcaa tgatgatccc
    14941 actgataccg acatcggcaa tttgcgagaa cgtcagacgg aagccataaa gaatgatacc
    15001 cagacgtaat aaatattgct tggcaaacag cacaccaccg tcacagcttt tccagatgtg
    15061 cggatagatg gtgttgccta aaaccatccc caacaagatt gcgagggtga gggcactaaa
    15121 cccggcaccc gcaaccgcgg gaatggaacc accccacagg gcgaccccgg tgataactgc
    15181 actcagggct aaccccggaa taaaatgcca cagtgtacga tgttgtttct gtaaggtgat
    15241 attcgtcata accctctcct ttaaccgggc ataaggttac gactaactgg tttaaaaata
    15301 aaattgatta tatatttata attaatcttt ataagtggta agcgactatg cacatcaccc
    15361 tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg
    15421 tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt gactgacctg gaagggcagc
    15481 ttggcgttca actgtttgat cgcgtgggga aaagactggt tgttaatgaa cacgggcggc
    15541 tgctctatcc gcgagcgttg gcactgcttg aacaggcggt tgaaatcgaa caactgtttc
    15601 gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac catcggtaac tacattctgc
    15661 ctgcagttat cgcccgttat cgccatgatt atccgcagtt gccgattgaa cttagcgttg
    15721 ggaacagcca ggacgtgatg caagcggtgc tggatttccg cgttgatatt ggctttattg
    15781 aaggaccgtg ccacagcact gaaatcattt ctgaaccgtg gctggaagac gagcaggtgg
    15841 ttttcgccgc gccgacttcg ccgttggctc gtggtccggt caccttagaa cagctggccg
    15901 ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg ggatattgtc gattatctgt
    15961 tgctgtcaca tttaccgaag tttgagatgg cgatggaatt aggtaactcc gaggcaatca
    16021 aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc gcgacgtgtg attgaagatc
    16081 aattgcaggc aggcacatta agtgaagttg cggtccctct gccgcgcctg atgcgtacgt
    16141 tgtggcgtat acatcatcgg caaaaacacc tttccaacgc gctacggcgc tttctggact
    16201 attgcgatcc cgcaaatgtg ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt
    16261 cgatttcgtg acgcagcgcc ttcagcatgc attcgccaga aaagagattg gctgctttac
    16321 ttataatccc tgggcgatca tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt
    16381 gccaatcgtc tgctacaatc gcgccatcat ttttaagatg gatagcattt ttgtatgggt
    16441 tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg
    16501 acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg
    16561 atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt
    16621 tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc cggttcgttt
    16681 gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac
    16741 tggtacaact gggcggtgac tattgccgtt gacctggttg cagcacagct ggtcatgagc
    16801 tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc
    16861 ctgttgaact acatctctgt tcgcggcttt ggtgaagcgg aatactggtt ctcactgatc
    16921 aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc
    16981 aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt
    17041 ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag
    17101 ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta
    17161 cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg
    17221 attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgtcagt
    17281 ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca
    17341 gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc tactcgtatg
    17401 ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt gtcgcgtggt
    17461 ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg cttcctgacc
    17521 tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt
    17581 tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg
    17641 cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc
    17701 ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa
    17761 gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt
    17821 atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag
    17881 ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag
    17941 agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt aatatttgga
    18001 ttgataattg ttatcgtttg cattatcgtt acgacacaat caaaaaaggc tgacaaatca
    18061 gaggctgttc cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg
    18121 ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt cgtagttacc
    18181 tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct gtgtttgtcc
    18241 gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc
    18301 actgcatctt ccgtggaaca aaatcttaaa gatgcacccg ccagtatcag cgtcattacc
    18361 caggaagacc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa agaagtgcct
    18421 ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg tggtctggac
    18481 agcagctaca ccctgattct tgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc
    18541 cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg tattgaagtg
    18601 gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc tcggcggtgt agtgaatatc
    18661 atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac caccattcag
    18721 gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg accattaatt
    18781 gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa ggatgacccg
    18841 caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt ctccagccgc
    18901 gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac tgccggatac
    18961 ggtttcgacc gtcaggatcg tgattccgat tcgctggaca aaaaccgcct ggaacgccag
    19021 aactactgcg tcagccataa tgggcgttgg gattacggca ccagcgaact gaaatcctac
    19081 ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc cgaaagcaat
    19141 acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct cacggttggc
    19201 ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg aaccagctcc
    19261 aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat cttcgagccg
    19321 ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga acactggagt
    19381 ccgcgtgcct acatggttta taacgccacc gacaccgtaa cggtgaaagg gggctgggcg
    19441 acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag caattcctgc
    19501 cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag cgaaagttgg
    19561 gagctggggc tttactacat gggtgaagaa ggctggctgg aagggggtga atccagcgtt
    19621 atcgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc tgacgtcaac
    19681 gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg acggcgcata
    19741 ccggtattta gctactacag cgttaacaaa gctcgtattc agggcgtgga aaccgaactg
    19801 aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa cgatggtcgt
    19861 gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac tgctaacggt
    19921 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
    19981 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
    20041 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
    20101 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
    20161 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
    20221 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
    20281 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
    20341 cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
    20401 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactat
    20461 cccattccag catgcatttt tgtcctcacc taaataggtg ctaaacgctt tgattcccca
    20521 cgggacgctg cacggattca caatgggcgc aaaggccgaa acgctggtgt atttacccgg
    20581 atttttcagc gccataatca gcgcaccgtg accacccatt gagtgaccgc taatggcgca
    20641 gcggtcgctg acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc
    20701 atacatccgg taatgcgtcg cccacggcgg ttgcgtggca ttaagataaa agcctgcgcc
    20761 ctggcctaaa tcgtagccat cgtcgttggc aaccttttcg ccgcgcgggc tggtgtctgg
    20821 catcaccagt acaatcccca gttccgccgc tacccgctgg gcacccgcct tggtggtgaa
    20881 gttctcgtca ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc
    20941 acgagggggt gggagaaaga tactgaacgt catcgggcag tttaaggtac tggagtcgtg
    21001 tcgccagcgt tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg
    21061 ctccttgttg tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta
    21121 actttcactt ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca
    21181 attacaataa ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca
    21241 agctgtaaat cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg
    21301 agaaatcata aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg
    21361 cgagaggact ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca
    21421 aaagccttat tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg
    21481 atgacagttt gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct
    21541 ccggtctgga ttacgccaat ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg
    21601 atgaaatggt caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta
    21661 ccatcgatgg caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa
    21721 aaattaaccg tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc
    21781 agcaaattct tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg
    21841 acgcggtgca ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacgacaa
    21901 cgacctctct tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg
    21961 ctgtgcgcca tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat
    22021 tttgttcgcg gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta
    22081 ccaaaggcgg cttatctcaa tcccgcctgg tacgatgcta ttacgccgca ggatgcatgg
    22141 acctgggcat ttctcgatct catcggccag gtgaaattcc tcacgctttt tgcgctgctg
    22201 tttggtgctg gcctgcaaat gttgctgccc cgtggcagac tctggatcca gtcgcggtta
    22261 acgctgttag tcttgttggg ctttattcac ggtttattgt tctgggacgg cgatattctg
    22321 ctggcttacg ggctggtggg gcttggcgtt ttgctgttat tggggctgat ttccgacagc
    22381 cagaccagcc gcgcctggac gccggatgca tcggctattt tgtatgaaaa atactggaag
    22441 cttcacggcg gcgttgaagc gatcagtaat cgtgccgatg gtgttggcaa cagtttactg
    22501 gcactgggcg cacagtatgg ctggcaactg gcagggatga tgctcattgg tgcggcgctg
    22561 atgcgcagcg gctggctgaa agggcagttc agcttacgtc actatcgtcg tactggtttt
    22621 gtgctggtgg cgattggggt gaccattaac cttcctgcca tcgccctgca atggcagctg
    22681 gactgggcgt atcgctggtg tgccttctta cttcaaatgc cgcgggaact gagtgcgccg
    22741 tttcaggcga ttggctatgc gtcgctgttt tatggcttct ggccgcaatt gagccgcttt
    22801 aagctggtgc ttgcgatcgc ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa
    22861 acgctgattt gtaccacgct tttttaccac ctcggtttgt ttatgcattt tgaccgcctg
    22921 gagctgctgg cgtttgttat tccggtatgg ctggcgaata tcctcttctc tgttatctgg
    22981 ctgcgttact tccgccaggg gccggtggaa tggctctggc gtcagttaac tttgcgtgct
    23041 gccggaccgg caatatctaa aacatcaaga taacgatctg gattacattc gttaacaaaa
    23101 cggctgtaac cgtttccatt gctgtgactc gattcacgaa gtcctgtatt cagtgctgac
    23161 aaaatagccg ccagcaagca gtcatttact gcaatctcat aacaggtagt gaatatgatc
    23221 accattcgtg atgtagcgcg tcaggctggc gtctctgtgg caacggtttc ccgggtgctc
    23281 aataacagca cgctggtcag tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag
    23341 ctggattatc ggccaaacgc caatgcccag gcgctggcaa ctcaggttag cgacaccatt
    23401 ggcgtggtgg tgatggacgt ttctgatgcg tttttcggcg cgctggtaaa agcggtggat
    23461 ctggtcgccc agcagcatca gaaatacgtg ctaatcggca atagctatca tgaagcggaa
    23521 aaagagcgtc acgccattga ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac
    23581 tcaaaagcat tgagtgacga tgaactggcg caatttatgg ataacattcc cggtatggtg
    23641 ttaatcaacc gcgttgtgcc ggggtacgcc catcgttgcg tttgcctgga taatctcagc
    23701 ggtgcccgaa tggcgacgcg catgttgctg aataacggtc atcaacgtat tggttatctt
    23761 tcttccagcc acggcattga agatgacgcc atgcgtaaag caggctggat gagtgcgttg
    23821 aaagagcagg atattattcc gccggaaagc tggattggca ctggtacgcc ggacatgccg
    23881 ggcggtgagg cggcgatggt tgaactgctg gggcgcaatc tacaacttac cgctgtattt
    23941 gcttataacg acaatatggc cgctggcgca ctgacagcat taaaagataa tggcattgcg
    24001 attccgttac atctctcaat catcggtttc gatgatattc ccatcgcccg ttacaccgac
    24061 ccgcaattaa cgaccgtgcg ttatcccatt gcttcaatgg ctaaattagc caccgaactg
    24121 gccttgcagg gggcagcagg caatattgat cctcgtgcca gccactgttt tatgccgacg
    24181 ttagtgcgtc gccattctgt cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca
    24241 aatcaggcga tgtaaccgct ttcaatctgt gagtgatttc acagtatctt aacaatgtga
    24301 tagctatgat tgcaccgttt taatgttgta acccgtatgt aacagtgaat aatcactttc
    24361 accgaggtaa tagcgtcata acaacaatta aagccgtttt ctggagcgtt accgggcatg
    24421 gaagaacgaa ttttaaaaag tgagcttcgg cgttcagtaa cacttcatta actctactgc
    24481 cccgccgagc atttatctca agcactaccc tgcataagaa aaaccggaga taccatgaat
    24541 aagaaggtgt taaccctgtc tgctgtgatg gccagcatgt tattcggtgc cgctgcacac
    24601 gctgctgata ctcgcattgg tgtaacaatc tataagtacg acgataactt tatgtctgta
    24661 gtgcgcaagg ctattgagca agatgcgaaa gccgcgccag atgttcagct gctgatgaat
    24721 gattctcaga atgaccagtc caagcagaac gatcagatcg acgtattgct ggcgaaaggg
    24781 gtgaaggcac tggcaatcaa cctggttgac ccggcagctg cgggtacggt gattgagaaa
    24841 gcgcgtgggc aaaacgtgcc ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg
    24901 gatagctacg acaaagccta ctacgttggc actgactcca aagagtccgg cattattcag
    24961 ggcgatttga ttgctaaaca ctgggcggcg aatcagggtt gggatctgaa caaagacggt
    25021 cagattcagt tcgtactgct gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc
    25081 acttacgtga ttaaagaatt gaacgataaa ggcatcaaaa ctgaacagtt acagttagat
    25141 accgcaatgt gggacaccgc tcaggcgaaa gataagatgg acgcctggct gtctggcccg
    25201 aacgccaaca aaatcgaagt ggttatcgcc aacaacgatg cgatggcaat gggcgcggta
    25261 gaagcactga aagcacacaa caagtccagc attccggtgt ttggcgtcga tgcgctgcca
    25321 gaagcgctgg cgctggtgaa atccggtgca ctggcgggca cagtactgaa cgatgctaac
    25381 aaccaggcga aagcgacctt tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct
    25441 gatggcacca actggaaaat cgacaacaaa gtggtccgcg taccttatgt tggcgtagat
    25501 aaagacaact tggctgaatt cagcaagaaa taagactgat tgggcgcagt cgtttactgc
    25561 gcccttattg tatggccaac aaggtaaaat tatatggtca gctcaacgac tccgtcttcc
    25621 ggggaatact tgttggaaat gagcggtatc aacaagtcct ttcctggtgt taaggcactt
    25681 gataacgtta atttaaaagt ccggccacat tctatccatg cattaatggg ggaaaacggt
    25741 gcaggaaaat cgacattatt aaaatgcctg tttggtattt atcaaaaaga ctccggcacc
    25801 attttattcc agggtaaaga gatcgatttc cattccgcca aagaggcact ggaaaatggt
    25861 atttcgatgg tacaccagga gttaaacctg gtattacaac gttcggtgat ggataacatg
    25921 tggctggggc gatatcccac caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa
    25981 accaaagcga tttttgatga actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca
    26041 ttatccgttt cgcaaatgca gatgatcgaa atcgccaaag cgttttccta taacgcgaaa
    26101 attgtgatta tggatgaacc gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc
    26161 actattattc gtaaattaaa agagcgcggc tgcggtattg tttatatctc gcataaaatg
    26221 gaagaaatct tccagttatg tgatgaagtt accgtattgc gcgacggtca gtggatcgcc
    26281 accgaaccgc tggcaggact gacgatggac aagatcatcg ccatgatggt tgggcgttct
    26341 cttaatcagc gttttcctga caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt
    26401 aacctaacgt cactgcgcca gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg
    26461 gagatcctcg gtattgccgg gctggtgggg gcgaaacgta ccgatattgt tgagacgtta
    26521 tttggtattc gcgagaaatc ggctggcacc attacgttgc acggcaaaca gatcaataac
    26581 cataatgcca acgaagccat aaaccacgga tttgcactgg taactgagga gcgccgctca
    26641 acgggaattt atgcctatct ggatattggt tttaactcgt taatttccaa tattcgcaac
    26701 tacaaaaata aagttggttt actggataac tcgcggatga aaagcgatac ccagtgggtg
    26761 attgattcga tgcgggtaaa aacgccgggt catcggacgc aaattggttc gctctccggt
    26821 ggtaatcagc aaaaggtgat tattggtcgc tggctactaa cgcaaccaga aatattaatg
    26881 ctcgatgaac cgacgcgcgg tattgatgtc ggggcgaagt ttgaaattta tcagttaatt
    26941 gccgaactgg cgaagaaagg caaggggatt attattatct cctctgaaat gcctgagttg
    27001 ttagggataa cagaccgtat tctggtcatg agcaatggtc tcgtttccgg aattgtcgat
    27061 acaaaaacaa caacgcaaaa cgaaattctg cgtcttgcgt ctttgcacct ttaagattag
    27121 gggcttccca tgagtgcgtt aaataagaaa agttttctta cttacctgaa agagggcggt
    27181 atttacgtcg ttcttttagt tttgctggcg attattattt tccaggaccc aacattttta
    27241 agtctgttga acttaagtaa tattctcacc cagtcatcgg tgcgtattat tatcgcgctc
    27301 ggtgtggcag ggttaattgt cacccagggg accgatcttt ctgctggtcg tcaggtaggg
    27361 ctggcggcag tggtggctgc gacattattg cagtccatgg ataacgccaa caaagtgttc
    27421 ccggaaatgg cgacgatgcc gattgcgctg gttattctga ttgtctgtgc cattggtgcg
    27481 gtgatcggtt tgatcaacgg tctgattatc gcttatctca acgtgacgcc gttcattacc
    27541 acgctcggca cgatgatcat cgtctatggc atcaactcgc tctattacga ctttgtcggg
    27601 gcgtcgccaa tttctggttt tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg
    27661 ctggggagtt tccgtctctc ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc
    27721 tgggtgttgt ggaacaaaac ccgcttcggt aagaacattt ttgccattgg cggtaacccg
    27781 gaagcggcaa aaatatctgg tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg
    27841 tctggcgtgt tctatgcctt tggcgggatg ttagaagccg gacgtatcgg ctctgccacc
    27901 aacaacctcg gctttatgta tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta
    27961 tcgttcagcg gcggtgtggg gacggtgatt ggcgtggtga ccggggtaat tatttttacc
    28021 gtcatcaact atggcctgac gtatatcggc gtaaacccat actggcagta catcatcaaa
    28081 ggggcgatta ttatcttcgc cgtagcgctg gattcactga aatacgcgcg taagaagtga
    28141 tgtgattgcg gatattgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    28201 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    28261 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    28321 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    28381 catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    28441 acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc ctgatcggcg
    28501 agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta ctgcataatg
    28561 ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc atcttcccag
    28621 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatt
    28681 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    28741 gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    28801 ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    28861 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    28921 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    28981 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagctgct
    29041 cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc gggtaatctt
    29101 ccttcagccg acgcagggcg gtcagattct cttccaacgg atgttcagca atctgctcca
    29161 tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga
    29221 cttcgttggc gataaaaaag ccgatcgttt taaacaccac accgccccaa cctgtgtcgt
    29281 aggctttggc acacatctca tagcagttgc ctaccggcga agaagagagg cagaacgggt
    29341 tgggaaagtt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg agcaagctcc
    29401 ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg catagaccac
    29461 ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc gggtctgata
    29521 attttgtgtt ttgatggtgt tacgctgctg ctctaactcc gcaaaggcat ccagtctggc
    29581 atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc ctgataaccg
    29641 tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc catcgatgat
    29701 cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa actcatctaa
    29761 ctcttcacgt gctacgcaag ttaccgcctg acagccgaga actttcagcg tgctggctac
    29821 gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc tgatatcgcc
    29881 ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcgc tatgctcaaa
    29941 cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc ggttttccgc
    30001 tttcagttgc tccagggtga gtgttttacc gatttcgtta ttgcacttaa tggtcacgcc
    30061 cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc ggaattgcgg
    30121 aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg tgacgtcata
    30181 accctggttt gtcagtgtca cactggcctg caatccggca ggacctgcgc caataatcgc
    30241 gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt gttgttcaaa
    30301 atcagtaaca aaacgctgta agcggccgat atcaatggga gcatcaacac cggcacgggt
    30361 acaaccgctt tggcataatt tctccgtcgg gcacactctg gcacaaacgg caccgagggc
    30421 attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga ttgagcgaat
    30481 aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt cgtgacataa
    30541 taaacagcga gaggcttctt taatcgccag taacggcgta aaagccggag tgagttcatc
    30601 cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta tggcgaacgc
    30661 taaataaata ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta agctgtgaaa
    30721 ggattcacaa atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaata
    30781 attattctca ataattaaca gaaaagagaa aatattaacg atagaaaata agataaacaa
    30841 tcatgccgat agataaaatt ccgaagccaa tatatgccac catataagcg aagaagagtc
    30901 cgggattttt tgctcgccag acatacaccg gttccggtgc atttttaacc cgtttatcaa
    30961 tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca aaatatcggg ataaggaaaa
    31021 aaacggcaac gacaaaaaac tgctgtacat ccataactac tttccttgtt tcttttgtaa
    31081 ttcaagtaac tgttcgggtg taacggcggg atacccctgc gcatcttccg gcacctggtg
    31141 catagccgga atagggacca gcggccctaa aaaacgcggt tcacgtttaa aaatataaag
    31201 gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata cgtactgaca gcatatcttt
    31261 cggtgacggt acggcataac actgagcctg aatccccata tgcagcgcaa taaataatgc
    31321 tcgctcacag tggaaacgtt gggtgataat aatgaaatca ttagtatcga aaactttgcg
    31381 tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa tcgagaacaa tatctgatgg
    31441 gtcgacacca gcagcgatta aatctttgcg catggtcatc ggctcgttat aactttgcaa
    31501 tgcgttatcg ccgctcagta atagataatt taccttaccg ctgttatagg cattaatcgc
    31561 tccttgaatg cggtagcgat aatactgatt aataacgccg gtacgataat attttgctgt
    31621 gccgagcacc acgccgacct ggcggtaggg gagatcctgc aattcgtcgt agatataagg
    31681 cgcggttttc cagctcatcc agcgatcgag gccgagcaca gtcaacagca gcaagccgat
    31741 caggactaac aggctgagga acacgcgctt taacatgcag gagactcgag ctaaggaaaa
    31801 tttccatcag gctactttac ccgctggttt tgcgcaagaa acggcaaact gtttgccggg
    31861 aatttcgaca ccaggagagg gctggcaagc cggacaaggc gttcacgccg catccggcac
    31921 caggcttaag cgagaagcac gcggtcgata ctgtgacagc cgagagcttt cagcgttgcg
    31981 gaggtggcat cccactgaat caacggcgca tcggcttttt ctgccagaac cgcgcgctgg
    32041 atatccgggt aatcataacc cttgagattc aacagaatta acgccccctg caacggcggc
    32101 agagtcgggt tgaatgcggc gttttcagcg tagctgccgc tgaaaatacg accgtcttta
    32161 cattccagcg cgacaccgct tggtgactta ctgtaaggca tgtgcgaacg gtttgccgct
    32221 gcaatcgccg cctgagaaag cgcatcacca gtcagcgcat agccgtgatc ctgctcgtcc
    32281 atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggcag atagtcacgc
    32341 agcgcgtgtg cctcgcggcc cggcaaatga atacgcagat ccagaccgct gttcagttca
    32401 ttcataaact gacggcagtg accacaaggc gtatagttaa cggtgatggc tgcaagcgct
    32461 ttttcaccac tcaaccaagc gtggctgatt gcgctttgtt cggcatgaac ggtttgctgc
    32521 attgttgcac caataaattc catattggca ccaaaatacc aggttccgct cacaccgcgc
    32581 gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc cgccagcgga
    32641 agtagtgcga atgccagcgc gtcttcgtcc agtcccgttg cgctcttcag cgatgagact
    32701 tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc cagtgcagat
    32761 tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat ggcgttgcct
    32821 cataatcatg taatgggata acagtttacg aacccgttat ggctttatat gtgatctgaa
    32881 tctcattaat tatgcaacac gtgaaactta catcaaaaat gcgcgacgta tcgcaaattt
    32941 tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga agtaattatc
    33001 ccgcataaca ccagcgctag cgaactaaat gcaccttcct gataatccag ctcggcgcag
    33061 cgcgccgtac cgagggcgtg cgaggcagtc cccattgcca gaccgcgcgc agctttggta
    33121 cgaatacgca tcgcattaag caatgtatgg ccaaataccg cgccgaggat gccgacgaaa
    33181 atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac cgccattgca
    33241 ataggcgtgg tgactgattt cggcaggatt gacgcggcga tttccggtga agcgcccatc
    33301 aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc tgccgataaa acagatggtg
    33361 atgatcgatt tccagcgcgc gcggatctgg tgtagctgct catataaagg ataggccagc
    33421 gcgacgaccg ccggttgcag cagatcgttt agcacttcgc tacctttaaa gtaactgtcg
    33481 taagagatgc cggtcagcat taaaaaagga atgatcacca ccattgctac cagcaacggg
    33541 ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg cggcaaaaaa gacaatcaac
    33601 gtcagcggta atgaccacca gatattcgcc atcattcttc tgatcctttc tgacctacga
    33661 ctttacgttc accgtgtacc agttgtgaac tccagctcac caccagaaaa accaccagcg
    33721 tactgacagc acaggaaacc actaccgggc caaactgcgc gcggagcaaa tcaaaatatt
    33781 gcatgacgcc tacgccaatc ggcacaaaca atagcgccat atagcgaatc agtacgtagc
    33841 accccggatt gacccatttc gccggaagaa tttgcaaggc cagcaggaca aacaggatct
    33901 gcatcccgat gatgctgccc ggaatggtta ccggtagcag ggaagcaatg aaaatgcctg
    33961 catacaggca ggcataaatc aggatgaaag cgcgtaaata ttgccagata atgttcagtg
    34021 tcttactcat aatcaacacc ccagatgaga tggattcatc atacaattaa ttcgtaaaac
    34081 gtgctaccga tcacattaag aaatcagcgc ttattttttc gctacccggt attacgcaat
    34141 gagcagcgaa ctcaggcata catcaagcat agagagggat tgcagtttgc agcaatccct
    34201 ctttgcagac ctttaatccg ctgaccatag gcgctaacat agcgcctcat ttttttgcgg
    34261 gtgatgatat gcgtgtgtta ctggcaccga tggagggagt gcttgactct caggtgcgtg
    34321 aattgctgac cgaagttaac gactacgatc tgtgcatcac cgagtttgtc cgcgtggtgg
    34381 atcaactgct gccggtaaaa gtctttcatc gcatttgccc tgagctacaa aacgccagcc
    34441 ggacaccatc tggtacgctg gtgcgcgtgc agttgttagg tcagttccca caatggctgg
    34501 cagagaacgc cgcccgtgcg gtggagttag gtttctgggg cgtggatctc aattgcggct
    34561 gcccgtcgaa aacggttaac ggtagcggcg gcggggcgac gttactcaaa gatcctgaac
    34621 tcatctacca gggggcaaaa gcgatgcgtg aagctgtacc ggcgcatttg cctgtcagcg
    34681 tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt tgaaatcgcc gatgcggttc
    34741 aacaggctgg cgctacggag ctggtggtgc atgggcggac gaaagagcag ggttaccgcg
    34801 cggagcatat tgactggcag gcgattggcg agattcgcca gcggctgaat attccggtga
    34861 ttgccaacgg tgaaatctgg gactggcaga gcgcgcaaca atgcatggcg atcagcgact
    34921 gcgacgcagt gatgattggt cgcggggcgt tcaatattcc caacctgagc cgggtggtaa
    34981 aatataacga accgcgaatg ccgtggccgg aggtggttgc tttgctgcaa aaatataccc
    35041 gtctggaaaa gcagggcgat accgggttat atcacgttgc gcggattaaa cagtggttga
    35101 gttatttgcg taaagaatac gatgaagcaa cggaattatt tcagcatgtt cgggtgttga
    35161 ataactcccc tgatattgca agggctattc aggcaattga tatcgagaaa ctctaaaaac
    35221 catagtgatg tttttgacaa tgtgttagct ctttatcaat aggccgcacg gttgtttggt
    35281 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg atgtgggctg
    35341 agggcgcgtt ttacagggat gttacctcgc gcccgtaccc gatagccgaa aggaataaga
    35401 tgagggaacc gcgccagcgg cgatttaccg ccgggagccc gggttgccag ggtggtggcg
    35461 attgagccac cctggcacgt tcaccggctt gagcgttcca tagtagcgag gaacatcaag
    35521 tgaacggaac agccttcgat cttacatatt cagaatataa tagtgccgcc gcgaacgcct
    35581 gatgcgacgc tttacgcgtc ttatcaggcc tacaggacag gtcacctggt aacctacatc
    35641 ttctccagca acttcttcac atctttgcca ttgcgggaat ctttattccg ctcggcccag
    35701 tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc gcagtaactg atccacttgc
    35761 aggctgtaat tgagttgctg ccagttgcca taaacccgca gcggtactgg cgtttctttg
    35821 aggaaatcaa tcagtttgct ttccccgttc cagccaccca cgacccgaat atcaaactgg
    35881 gtgtcgcagg tttgatctgc cagattcaac atgccttccc ctgtcagcgc cagcacaggc
    35941 gattgacctt gcatgtcgtt taacgtcacg acgccatcct tcaacgtcaa atcggtggta
    36001 aagcggtcaa gccgcgtcac gttatcgaag ttttcagcgg ccttcacatc accgccatta
    36061 cgctctaccg cttgctgaat catctgctgg aagttcatcc cttccatacg tgtgtcggtc
    36121 atctcaacat gcgcctgtcc ttgccagttg cggcggaatg cgtcggcatc tatgtcagca
    36181 ccggagaaat caccagccag tgacattttt ccggtcaacg aaatcggata gttaaacgcc
    36241 ttcagaatgg taccaatctc aacgttctcc agccgtggct ggaagtttat ccgcggattt
    36301 attgatgtcg cgtccagcgt gcccggcagt gaaacctttc caccgttaag cttgccctgc
    36361 agttgagtaa tttccagcaa acccgacttg ttggtcattt gcgtggcaac atctgtaaaa
    36421 ttcattccgc gccagcgcac gttactggcc tgcaacaaaa tatcagctgt aaagccttgc
    36481 agtccctgat aggccggttc atcaatacgc gaagaaatga ccgggcgcgg caacgttgat
    36541 tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg cggtttcatt aagcgggatg
    36601 aggttgtcaa gattcagttg cgggaattgc agtctcagct gccattccgg tttctctgtc
    36661 agcgtgacct gtgcttgtcc gctcagcgta ctgtcattgg cggtcaaact aatttggtta
    36721 aatgaaaggc gtttatgtga ctcctgccac tgggactgga aactcccctg cccctgaata
    36781 ccttgttttg gtaaatcggc accctgcaac tgccagttaa tttgttcaat agccgctgtt
    36841 aaatcatgcg gataatcaga agcatctacc gtaccgttaa gggatatcgt gagatcgcgc
    36901 tgatcgcgat taacccgccc ggagaactca aatgagccac gatgttgggg atcttgttcc
    36961 atttgcaggc ggatattgcg gattgtcacc tgctcgtcat cttcatgctg gaacaccaga
    37021 acgctgtccg ccaccttaag actggatata tcaaacgacc atccgcgatc gtctgacaga
    37081 tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc
    37141 tgcggcgtca gttggatcac tgcccctttt agcatcacct gcttaacgct cagttgatga
    37201 ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg
    37261 cttgcgccct gggcggtgag agacattcgc ccggagagga tactaagctg cggccagacg
    37321 tgccaacgca gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc aacttgcttg
    37381 accatatagt cgcggaaatc attcggattc accagcaaca ctaacgcaga taacccggcc
    37441 accagcacga ccaagagtat catcagcgtc gtcagaaatc gtctcatggt atcctcaatg
    37501 ggcctgaatt agtctttatc gattcggctg gctaccgcgc cctgctggtt gcgatatttc
    37561 gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc
    37621 gcaccaatta acatgcccgg acgcagcgcc agcggcagct taccggagtt gtagaactcc
    37681 agcacaatgc aaccagacca gcccggatcg atacggtgcg cggtgacgtg caccatcagc
    37741 cccagacgcg ccaaagagga acgcccgtcc agccagccca ccagatcggc tggcagcgtc
    37801 accgactcca gtgtcaccgc cagcgccagc tctcctgggt gaagatagaa cgcctcgccc
    37861 tcgtcgagaa cgatctcatc gctcatcacg cggtcaagcg cggcgctcac ttcatctttg
    37921 ggaccgctca gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa tttattgccc
    37981 aggcgtacat ccaccgtcgc gccgttaata cgctccactg gcggacgtgg gttgatcgac
    38041 aaacggcctt catcaagcca ggcttcaata tctcggtcac acagacgcat ggcattttct
    38101 cctttcgcac atcactccct taacgccaat tacgtcaagg gcataccaac ttatcactga
    38161 acggtacaca atttatcaag cttattcaaa gaactgactt attttcgctt tcaatatatc
    38221 gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg tccgcatatt gtttagaagg
    38281 ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga tattgcgcca tcacggaatc
    38341 cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc agatatccag
    38401 cggggtatca acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa
    38461 caaaatgcct tcgagaatga tgaccttctt cggctcaacc gtcaccgttt ctttcatacg
    38521 cgtatgttca acatagctgt aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg
    38581 taaatgctca agcaacaggc tgtgatccat cgcgctggga tggtcgtagt tggtcttaac
    38641 gcgttcttcc atcgacagat ggctttgatc tttgtaatag cagtcttcgg gaattacgcc
    38701 gatgtgttca tcaccgactt gctcacgtaa ttcacgataa agggtactgg caataagact
    38761 cttgccggaa gccgatgcgc cagcgatacc gataatgacg cactgatgag actgatcagt
    38821 catatattta gcgacctgat taacctggat gttaggaagg gggcgacgaa gcgccaaacg
    38881 cggcaattat agggatttca tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag
    38941 agttaattgt tgacaaagaa ttcccggtgg caaattacgt tgatcagttt tatgtaaggt
    39001 aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta cgtcacttta tgagcaattc
    39061 gcatataaaa tgtaaaactt ttgtactagc ataaacacag aaacgaatac tggcgacctg
    39121 gccttgcgga taaagcggta ataagcaaac aatcacagca tgtattaatt gccctgcccc
    39181 acccgctgct tcacctggtc agtttaggtt tagtctcgtt tatctttacc cttgtctcgc
    39241 ttgagctttc gcagtttggc acccaactcg ccccactgtg gttcccgacg tccatcatga
    39301 tggtggcgtt ttatcgccat gccgggcgca tgtggccggg aattgcgctg agctgttcgc
    39361 tgggaaatat cgccgcatcc atcctgcttt tttccaccag ctcgctgaac atgacctgga
    39421 cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt
    39481 ggtataaccc cttgcaaaat ctggctgact ggctgcgtct ggcattcggc agcgccattg
    39541 ttccgcctct gt
//
LOCUS       DADLCQ010000025        38861 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000025 DADLCQ010000000
VERSION     DADLCQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.025"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1546
                     /locus_tag="JQG95_001148"
     CDS             <1..1546
                     /locus_tag="JQG95_001148"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY5887778.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
     gene            1705..2264
                     /locus_tag="JQG95_001149"
                     /pseudo
     CDS             1705..2264
                     /locus_tag="JQG95_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            2265..3914
                     /locus_tag="JQG95_001150"
     CDS             2265..3914
                     /locus_tag="JQG95_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY5887779.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVTRVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="JQG95_001151"
     CDS             3919..4695
                     /locus_tag="JQG95_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY5887780.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="JQG95_001152"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="JQG95_001152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY5887781.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TVMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JQG95_001153"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JQG95_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY5887782.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JQG95_001154"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JQG95_001154"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY5887783.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            12096..13217
                     /gene="ompC"
                     /locus_tag="JQG95_001155"
     CDS             12096..13217
                     /gene="ompC"
                     /locus_tag="JQG95_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY5887784.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13329..14384
                     /gene="apbE"
                     /locus_tag="JQG95_001156"
     CDS             13329..14384
                     /gene="apbE"
                     /locus_tag="JQG95_001156"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY5887785.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14458..15522
                     /gene="ada"
                     /locus_tag="JQG95_001157"
     CDS             14458..15522
                     /gene="ada"
                     /locus_tag="JQG95_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY5887786.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15522..16172
                     /gene="alkB"
                     /locus_tag="JQG95_001158"
     CDS             15522..16172
                     /gene="alkB"
                     /locus_tag="JQG95_001158"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY5887787.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            16248..17891
                     /gene="yojI"
                     /locus_tag="JQG95_001159"
     CDS             16248..17891
                     /gene="yojI"
                     /locus_tag="JQG95_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY5887788.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18109..19721
                     /gene="mqo"
                     /locus_tag="JQG95_001160"
                     /pseudo
     CDS             18109..19721
                     /gene="mqo"
                     /locus_tag="JQG95_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(19871..20359)
                     /gene="eco"
                     /locus_tag="JQG95_001161"
     CDS             complement(19871..20359)
                     /gene="eco"
                     /locus_tag="JQG95_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY5887789.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(20494..20658)
                     /locus_tag="JQG95_001162"
     CDS             complement(20494..20658)
                     /locus_tag="JQG95_001162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887790.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            20767..21261
                     /gene="napF"
                     /locus_tag="JQG95_001163"
     CDS             20767..21261
                     /gene="napF"
                     /locus_tag="JQG95_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY5887791.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21251..21514
                     /gene="napD"
                     /locus_tag="JQG95_001164"
     CDS             21251..21514
                     /gene="napD"
                     /locus_tag="JQG95_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY5887792.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21511..23997
                     /gene="napA"
                     /locus_tag="JQG95_001165"
     CDS             21511..23997
                     /gene="napA"
                     /locus_tag="JQG95_001165"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY5887793.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELVEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            24004..24699
                     /gene="napG"
                     /locus_tag="JQG95_001166"
     CDS             24004..24699
                     /gene="napG"
                     /locus_tag="JQG95_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY5887794.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24686..25549
                     /gene="napH"
                     /locus_tag="JQG95_001167"
     CDS             24686..25549
                     /gene="napH"
                     /locus_tag="JQG95_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY5887795.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25546..25995
                     /gene="napB"
                     /locus_tag="JQG95_001168"
     CDS             25546..25995
                     /gene="napB"
                     /locus_tag="JQG95_001168"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY5887796.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            26005..26607
                     /gene="napC"
                     /locus_tag="JQG95_001169"
     CDS             26005..26607
                     /gene="napC"
                     /locus_tag="JQG95_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY5887797.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26626..27243
                     /gene="ccmA"
                     /locus_tag="JQG95_001170"
     CDS             26626..27243
                     /gene="ccmA"
                     /locus_tag="JQG95_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY5887798.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTTIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            27240..27902
                     /gene="ccmB"
                     /locus_tag="JQG95_001171"
     CDS             27240..27902
                     /gene="ccmB"
                     /locus_tag="JQG95_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY5887799.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            27944..28681
                     /gene="ccmC"
                     /locus_tag="JQG95_001172"
     CDS             27944..28681
                     /gene="ccmC"
                     /locus_tag="JQG95_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY5887800.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            28678..28887
                     /gene="ccmD"
                     /locus_tag="JQG95_001173"
     CDS             28678..28887
                     /gene="ccmD"
                     /locus_tag="JQG95_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY5887801.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            28884..29363
                     /gene="ccmE"
                     /locus_tag="JQG95_001174"
     CDS             28884..29363
                     /gene="ccmE"
                     /locus_tag="JQG95_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY5887802.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            29360..31303
                     /locus_tag="JQG95_001175"
     CDS             29360..31303
                     /locus_tag="JQG95_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY5887803.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            31300..31857
                     /gene="dsbE"
                     /locus_tag="JQG95_001176"
     CDS             31300..31857
                     /gene="dsbE"
                     /locus_tag="JQG95_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY5887804.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEKIKPLWEKYSKEAAQ"
     gene            31854..32906
                     /locus_tag="JQG95_001177"
     CDS             31854..32906
                     /locus_tag="JQG95_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY5887805.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(33117..33764)
                     /gene="narP"
                     /locus_tag="JQG95_001178"
     CDS             complement(33117..33764)
                     /gene="narP"
                     /locus_tag="JQG95_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY5887806.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            34084..36675
                     /gene="yejO"
                     /locus_tag="JQG95_001179"
     CDS             34084..36675
                     /gene="yejO"
                     /locus_tag="JQG95_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY5887807.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(38076..38360)
                     /locus_tag="JQG95_001180"
     CDS             complement(38076..38360)
                     /locus_tag="JQG95_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY5887808.1"
                     /translation="MSMIHDSKAEDLESKGLYRRAAARWAEVMQQVNTDKEREQAVKR
                     RAECIHKAARSPVILDNFGGLREAANRTQADMGIEIAKSVWRNYQTDKVL"
     gene            complement(38357..>38861)
                     /locus_tag="JQG95_001181"
     CDS             complement(38357..>38861)
                     /locus_tag="JQG95_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HAY5887809.1"
                     /translation="DSIYGIPKKKTQTTQQDKTTSAHKVIPGNQNAPQSRQQKPTGAT
                     PWRHMTKRQGKNRRRANRLIELWPELFSLESPKPLKVGIFEDLIQDIAVRAQAFGSGA
                     LRAALASYAHSPRYYRALMAGGVRYDLNSQPCGEVTQQEQQNAEARLMALKEKRKRKS
                     NKEQAGV"
BASE COUNT         9067 a         9456 c        10746 g         9592 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctctagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagccga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttaccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggtaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tattgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttattt
    11881 cgccattccg caataatctt aaaaagttcc cttgtattta cattttgaaa catctatagc
    11941 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
    12001 tggggagaat ggacttgccg actgattaat gagggttaat cagtatgcag tggcataaaa
    12061 aagcaaataa aggcatataa cagagggtta ataacatgaa agttaaagta ctgtccctcc
    12121 tggtcccagc tctgctggta gcaggcgcag caaacgctgc tgaagtttac aacaaagacg
    12181 gcaacaaatt agatctgtac ggtaaagtag acggcctgca ctatttctct gacgacaagt
    12241 ctgtagatgg cgaccagacc tacatgcgtc ttggcttcaa aggtgaaact caggttactg
    12301 accagctgac cggttacggc cagtgggaat atcagatcca gggcaacagc gctgaaaacg
    12361 aaaacaactc ctggacccgt gtggcattcg caggtctgaa attccaggat gtaggttctt
    12421 tcgactacgg tcgtaactac ggcgtagttt acgacgtaac ttcctggacc gacgtactgc
    12481 cagaattcgg tggtgacact tacggttctg acaacttcat gcagcagcgt ggtaacggct
    12541 tcgcaaccta ccgtagcacc gacttctttg gtctggttga cggtctgaac ttcgctgttc
    12601 agtatcaggg caaaaacggt agcccggaag gtgaaggcat gaccaacaac ggtcgtgaag
    12661 ctctgcgtca gaacggtgac ggcgttggcg gttctattac ttatgattac gaaggcttcg
    12721 gtatcggtgc tgcagtttcc agctccaaac gtactgatga ccagaacttc ggtctgaacc
    12781 gttatgatga gcgttacatc ggtaatggcg accgcgctga aacctacacc ggtggtctga
    12841 aatacgacgc gaacaacatc tacctagctg ctcagtacac ccagacctac aacgcaactc
    12901 gcgtaggcaa cttgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc
    12961 agttcgactt cggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg
    13021 gtgtcatcaa tggtcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg
    13081 cgacctacta cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg
    13141 acgacaacca gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc
    13201 tggtttacca gttctaatct cgatggatat cgaacaaagg gcctgcgggc ccttttttca
    13261 ttgttttcag cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat
    13321 aatcacgtat ggaaataagc tttacccgcg tggcactgct ggttgccgcg ctcttctttg
    13381 ttggttgcga tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg
    13441 aaggcaaaac catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca
    13501 gtgccgaact taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga
    13561 cctataaaaa agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc
    13621 cggtaagtga agcgatggcc gatatcgtca ccacctcgct gcgcattggg gcaaggaccg
    13681 atggtgcgat ggatattacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac
    13741 aacagccggt tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct
    13801 tacagcacct gacggtcatc aatcagtcgc atcagcaata tctgcaaaaa gacctgccgg
    13861 atttatatgt cgatctctcc accgtcggtg aaggttatgc ggcggatcac ctggcacgct
    13921 tgatggagca ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc
    13981 gtggtatgaa cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag
    14041 aaaacgcggt tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct
    14101 accgtaacta ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg
    14161 ggcgtcccat cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag
    14221 ccgatgcctg ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc
    14281 gccgggaagg gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga
    14341 tgtcaccaca gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg
    14401 tttttgcgtg atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg
    14461 aaaaacgcca catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg
    14521 aatgccgacg gcgaattcgt tttcgccgtg cgcaccacgg gtatcttttg ccgtccttct
    14581 tgccgcgcca gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca
    14641 ctcgccgccg gatttcgccc ctgcaaacgt tgtcagccag acaaagccaa tccccggcaa
    14701 catcggttgg ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg
    14761 ctggaagcct tagccgacca ggtggcgatg agtccatttc atctacatcg gttgtttaaa
    14821 gctactaccg gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc
    14881 gaatcgctgg cgaaagggga gagcgtgacg acgtctattc ttaacgccgg attccccgac
    14941 agcagcagtt actaccgcaa agccgatgaa acgctgggca tgacggctaa acaattccgt
    15001 cacggtggcg aaaatctggc ggtgcgttac gcgctggctg attgtgagct gggtcgttgc
    15061 ctggtggcag aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca
    15121 ttaatcagcg agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg
    15181 tttcagcaac atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg
    15241 ttaccgctgg atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg
    15301 ataccttgtg gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa
    15361 gcggtacggg ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt
    15421 catcgagtgg tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa
    15481 gcgcaactgc tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg
    15541 atgctgaacc gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt
    15601 ttaatgctgc ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc
    15661 gccagatggt cgctccaggc ggatatacca tgtcggtggc gatgaccaac tgtgggcatc
    15721 tgggctggac gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata
    15781 aaccgtggcc cgccatgcca caaagctttc atgatttatg tcaacgtgcg gctacggcgg
    15841 cgggctatcc agatttccag ccagatgctt gtcttatcaa ccgctacgct cctggcgcga
    15901 aactgtcgct gcatcaggat aaagacgaac cggatctgcg cgcgccaatt gtttctgttt
    15961 ctctgggctt acccgcgatt tttcaatttg gtggcctgaa acgtaatgat ccgctcaaac
    16021 gtttgttgtt ggaacatggc gatgtggtgg tatggggcgg tgaatcgcgg ctgttttatc
    16081 acggtattca accgttgaaa gcggggtttc atccactcac caccgactgc cgctacaacc
    16141 tgacattccg tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc
    16201 gaagatgttt aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg
    16261 tacttgtctg gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca
    16321 gtgcggcatt aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg
    16381 ataccagtct gctggtgtta ccggagtttc tgggattatt gctgctgttg atggcagtca
    16441 ctctcggatc gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta
    16501 gtgaatttat caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg
    16561 cctcgttgct ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc
    16621 tgccggaact ggggcagggg atcattctca ctatcggttc atcggcgtat ctgtggatgc
    16681 tgtcgggcaa aatgttgctg gtaacggcta tctggatggc gatcaccatc tggggcggtt
    16741 ttgtgctggt ggcgcgggtg tacaaacata tggcgaccct acgtgaaacc gaagacaagc
    16801 tgtacacgga ttttcaaact gtacttgaag ggcgcaaaga gctgactctg aaccgggaac
    16861 gcgccgagta tgtgtttaac aacctctaca tccccgatgc gcaagagtat cgccatcata
    16921 ttattcgcgc agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg
    16981 gcgctatcgg cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg
    17041 ctgcgaccta ttcgttgacg ctgttattcc tgcgtacgcc gctgctttcg gcggttggcg
    17101 cattgccgac gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg
    17161 cgcctttcaa agctgagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc
    17221 tgcgtaacgt gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca
    17281 ccatcaaacg tggcgagctg ctgtttctga ttggcggtaa cggtagcgga aaatcgacgc
    17341 tggcgatgtt gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca
    17401 aacctgtcag cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg
    17461 atgtctggct gtttgatcaa ctgctggggc cggaggggca acccgctaac ccgcaactgg
    17521 ttgagaagtg gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta
    17581 ttgttaacct gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg
    17641 cagaagaacg cgatattatc ctgctggatg aatgggcggt ggatcaggat ccacacttcc
    17701 gtcgtgagtt ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg
    17761 ctatcagtca tgatgatcat tactttatcc acgccgaccg cctgctggaa atgcgcaatg
    17821 ggcaacttag cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc
    17881 ggacggcata atattacgac gctgacctgg cggactgctg ccgtcaggtc aatatgctaa
    17941 aaatcccttc atttctaata accctataat taatcaacga aattataatg tttctaaaat
    18001 tagaatataa tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg
    18061 cgcaaatctc acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg
    18121 actgccatgc tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg
    18181 aaagcgtccg aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc
    18241 acgttgggga cctattgacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc
    18301 ctggaggggg gcgcgcagga gagttcgaac ggctagaaca acgccggaac cgggcattct
    18361 gcactgatgg aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa
    18421 gcagtcgcca ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggtcgag
    18481 cgcggcgtgc tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc
    18541 tggggcgagg ataacgttaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg
    18601 ctgtttcgcg gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta
    18661 gtgatggaag ggcgcgatcc gcaacagaaa ttggcactga tgtgaactat ggtgagatca
    18721 cccgccagtt aattgcttcc ttacagaaga aatctaactt ctcgctgcaa ctcagcagcg
    18781 aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt gccgatctga
    18841 aaaacggcac tgcacagaac atccgcgcca aatttgtctt tatcggcgcg ggcggtgcgg
    18901 cgctgaagct gttacaggaa tcggggattc cggaagcgaa agactacgcc ggtttcccgg
    18961 tgggcggaca gttccttgtt tcggaaaatc cagacgtggt taatcgccat ctggcgaaag
    19021 tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc gatacccgtg
    19081 ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca accaaattcc
    19141 tcaaaaacgg ttcattgtgg gatctgatga gttccaccac cacctctaac gtgatgccga
    19201 tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt caggtgatgt
    19261 tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaagcg aaaaaagagg
    19321 actggcgttt gtggcaagcg gggcagcgtg tgcagattat caagcgtgat gccgagaaag
    19381 gtggcgtact gcgtctgggt accgaagtgg tcagtgacca gcaagggacc attgcggcgc
    19441 tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttgaat ctactggaaa
    19501 aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc
    19561 cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag ttgcagtaca
    19621 ccagcgaagt gctggggctg aaatacgaca aaccgcaagc agcagatagt acgccgaaac
    19681 cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc acatccggca
    19741 tggtatgccg gatgtggcgt atactgataa gacgcgccag cgtcgcatca ggcaaccggc
    19801 tcgggcgtta gatgttggat gcggcgtaac cgccttatcc aaccaacggg gtgccgtact
    19861 tcacaacagt ttagcgaact tccgcgttgt caattttctc ttccgccttc cagacgcggt
    19921 atttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg ttgtaacgca
    19981 gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg ccatccgggc
    20041 aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa gacataataa tcatagcccc
    20101 agccttccag cgttttgctt tccagcttcc cgccgagacg atgcaaattg caatcgactt
    20161 ccagcgtctg accgattaac agttctactt tcagggtaga ttcatcttct tgcggggtta
    20221 actgaatcac ctgacgcttc atgcctgttt cagcttgtgg ataaggcgcg attttttcca
    20281 gtggctggac gctttctgcc gcccaggcgg aagtggtagc gaaagcggca aacaatactt
    20341 caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt tcaccgatga
    20401 tgtcgctgaa ggttaacata ttcatcagat ttattaattt gctttatgaa taacttaaag
    20461 aacattcaga caattttacc tgctctttta gtgttaaatt ctgatgaggg agcaaaccag
    20521 cagaaatatc tctggtgaat taatcgctat ataaatatgt ttataaccat ttgaaatgtg
    20581 agcaaaagcc cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac
    20641 cccgatcggg gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc
    20701 ttattaatta tgcggtctat ttggtcgctc tcaattttca gagcgcgtta atgatggaag
    20761 gtcaatgtga agattgatgc atcccgtcgg ggcatactca ctggttgctg gcgcaaagcc
    20821 agtaacggta tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc
    20881 cgctgcgacg cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cacgggtggc
    20941 tatccgagcg ttaatttcaa aaataacgag tgcagcttct gttacgcctg cgcgcaggct
    21001 tgccccgaat cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatc
    21061 ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag
    21121 ccaatggcaa ttatctttcg cccgacgctc tccgggatct accaggcgca acttaatagc
    21181 caactttgca acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg
    21241 gagtatctcc atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga
    21301 acgaatttca gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag
    21361 cgacgcgccg agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat
    21421 ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca
    21481 ccagcaggaa gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa
    21541 gctaacgccg ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc
    21601 gccgttgttg gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt
    21661 actggttgcg gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac
    21721 ccggacgcac cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc
    21781 atgtacggta aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac
    21841 aaagaaggcg aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa
    21901 ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag
    21961 tggactatct gggaaggtta tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac
    22021 aacatcgacc cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc
    22081 tttggtatgg atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg
    22141 ctgtggggcg ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaacagt
    22201 cgtctctcta accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc
    22261 gagctggcgg ataacggcat catctttacg ccgcaatctg acctggtgat cctgaactac
    22321 atcgccaact atatcattca aaacaatgcg ataaatcagg acttcttcag caagcacgtt
    22381 aacctgcgca aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa
    22441 aaagcagcga agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa
    22501 gccttcgttg ctgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac
    22561 cagttagaac aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg
    22621 acgatgggct tcaaccagca tacccgtggt gtgtgggcta acaacctggt ctacaacctg
    22681 cacctgctga ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcaa
    22741 ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac
    22801 atggtggtaa ctaacgagaa acatcgtgat atctgtgaga agaagtggaa tatcccgagc
    22861 ggcaccattc cggcgaaaat cggtcttcat gcggtggcac aagaccgtgc gctgaaagac
    22921 ggcaagctga atgtttactg gaccatgtgt accaacaaca tgcaggccgg gccgaacatc
    22981 aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg
    23041 tatccgacag tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag
    23101 aaagagggcg cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaggtacag
    23161 gcgccaggcg aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa
    23221 actgaagaag tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg
    23281 ctgtacgaag ttctgtatgc cacccccgaa gtgagcaaat tcccggtatc cgaactggtg
    23341 gaagatcagc tgaacgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc
    23401 gaagagtacg catggtttgg tcgcggtcac ggtcacgatc tggcaccgtt cgatgactac
    23461 cacaaagcgc gcggtctgcg ctggccggtg gtgaatggta aagaaacaca gtggcgttac
    23521 agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg
    23581 gatggcaaag cggtgatctt cgcactgccg ttcgaaccgg cggcggaagc accggatgaa
    23641 gagtacgacc tgtggctctc taccggacgc gttctggagc actggcacac cggcagtatg
    23701 actcgccgtg tgccggaact gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg
    23761 ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt
    23821 ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg
    23881 tacatgccgt tcttcgacgc cgcacagctg gttaacaaac tgacgctgga tgcgaccgat
    23941 ccgctctcga aagagacgga cttcaagaag tgcgcggtca aactggagaa ggtgtaagcc
    24001 attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgtcgtt
    24061 cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca
    24121 cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt
    24181 gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg
    24241 acgctggcct ctggtctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc
    24301 gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag
    24361 attgaatcga tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt
    24421 ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag
    24481 gccatcacct tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg
    24541 acggttcaca gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa
    24601 caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac
    24661 cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc
    24721 gctggagaaa aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca
    24781 gttcttcgtg ctggggatgt ttttgagcgg tccatggttt ggtgtgtgga tcttgcacgg
    24841 caactacagc agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct
    24901 gcaaagtctt gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat
    24961 caccgtgctc tacgccctgg cgggaaagcg attattttgc agctgggtct gcccgctgaa
    25021 cccgattacc gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac
    25081 gatccctcgc cacattcgct acgtgctgtt ggtggtcatt ctggtgggat cagccttaac
    25141 tggcacgctc atttgggaat ggatcaaccc cgtttctctg atggggcgta gcctggtaat
    25201 gggcttcggc agcggcgcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt
    25261 tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag
    25321 caaaggtgtg attaccgttg cggccagcga tcggcagaaa tgtaaccgct gtatggattg
    25381 ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc
    25441 ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga
    25501 ggatgtattt acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac
    25561 ctgaagaaag cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt
    25621 tgggcggcta atggcgtcga ttttagccag tcgccggaag tatccgggac gcaggaaggg
    25681 gccattcgga tgccaaaaga gcaggatcgg atgccgctga actatgtgaa tcagccgcca
    25741 atgatcccgc atagcgttga aggttatcag gtaacgacca ataccaatcg ctgcttgcag
    25801 tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt
    25861 atggacagcg acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa
    25921 tgtcacgtac cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa
    25981 ggttacggga aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag
    26041 cgcctgtgga aatggtggcg tacccccagc catctggcgc tggggacgct gctgttgatc
    26101 ggttttgttg gcggcatcgt cttctggggg ggctttaaca ccgggatgga aaaagccaat
    26161 accgaagagt tctgcattag ctgccacgaa atgcgcaaca cggtgtatca ggaatacatg
    26221 gattccgtgc actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt
    26281 ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt
    26341 aaaatttttg gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca
    26401 cagaatgagt ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac
    26461 ttcgagtata tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg
    26521 gtgaaagatg ggcaaacctg tattgattgc cataaaggga tcgcgcacaa gctgcccgat
    26581 atgcgtgaag tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga
    26641 gagttacttt gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac
    26701 gcaggagagt gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt
    26761 ttgctgacgg ggttgtctcg ccctgacgct ggcgaggttc tctggcaagg gcagcccttg
    26821 catcaggtac gcgacagcta ccatcaaacc ctgttatgga taggccatca gccggggatc
    26881 aaaacccggc tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgatgccgca
    26941 caatgtctgg aagccttggc gcaggccggg cttgccggat tcgaagatat tccggtaaat
    27001 caactttctg ccgggcaaca acgccgcgtc gctttagcgc gtctgtggtt gacccgtgcc
    27061 acattatgga tcctcgacga gccttttacc acgattgacg ttaacggcgt cgatcgtctg
    27121 acccagcgta tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag
    27181 ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gagggccgca
    27241 tgatgttctg gcgtattttc cgtcttgaac tgcgcgtagc gtttcgccat agcgccgaaa
    27301 tcgccaaccc gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc
    27361 cggagccgca actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt
    27421 catccttgct ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac
    27481 aattgatgtt gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact
    27541 ggatggtaac cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa
    27601 tggatgttta tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg
    27661 gctttctcgg tgcaccgggc gtggcgctga cagtgggact taagcgcggt ggtgtgctgc
    27721 tcagcatact ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg
    27781 acgcggcttc tatgcatttg cccgttgatg ggtatctggc aattttaggc gcgttgctgg
    27841 caggcaccgc gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat
    27901 aacgctggat tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca
    27961 tcaactggcg atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc
    28021 aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc
    28081 tgattatcag cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg
    28141 gtcgatgggc atttatgcat caatggcggt ggcggcgttt attggccttg tctggcagat
    28201 gaaaatggcc aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat
    28261 tgccctggtt accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga
    28321 tgcacgtctg acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg
    28381 gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg
    28441 cgtggtgaat ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg
    28501 atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat
    28561 ttttggcttc ctgctcctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt
    28621 gctgatggaa aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg
    28681 acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc
    28741 tggctggcgg tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg
    28801 caacatcgcg caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt
    28861 gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct
    28921 gtgccgtgtt ggcagggcta gcgctgacta tcggtctggt gctatatgcg ctgcgctcga
    28981 atatcgatct cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaga
    29041 tgccggaagt cggtcagcgt ctgcgcgttg gcgggatggt gatgccgggt agcgtgcagc
    29101 gcgatcccaa ttcgctgaaa gtgaccttca ccatttacga tgctgaaggc tcagtggatg
    29161 tctcttacga aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg
    29221 gcgagctgga aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa
    29281 actacacgcc gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg
    29341 tttataagga cccagcatca tgatgccgga aattggaaac gggctgctgt gcttggcgtt
    29401 agggattgcg ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg
    29461 catgatggcg tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt
    29521 tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa
    29581 ctccaatacc cagcttccgg tgtggtatcg cgtggcggct acttggggcg cgcatgaagg
    29641 ttcgcttctg ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag
    29701 tcagcgtatt ccgctggata ttgtggcccg tgtactggcg ataatgggga tggtcagcgt
    29761 cggctttttg ctgttcattc tctttacctc taacccgttc tcacgcacgc tgccgaactt
    29821 cccgattgaa gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc
    29881 gcctctgctt tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc
    29941 tttgctgagc gggcgtctgg acagcactta tgcgcgtttt actcgcccgt ggacgctggc
    30001 ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact
    30061 cggctggggt ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct
    30121 ggtggggact gcactgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc
    30181 gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt
    30241 gcgttccggc gtgctggtat cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt
    30301 tatcctcgcc tttatggtgc tggtgattgg cggttcgctg ctgctgtttg ccgcgcgtgg
    30361 acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg cgggaatctt tgctgttagc
    30421 gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg ctggggacgc tgctgccgct
    30481 ggtgcacaag caacttggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat
    30541 gtttacctgg ctgatggtgc cgtttgcgct actgcttggt gtcggtcctc tggtgcgctg
    30601 ggggcgcgat cgcccacgta aaatccgcaa tttattgatt atcgccttca tctctacgct
    30661 ggtgctgtct ctgctgttgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct
    30721 cggcctggca atggcctgct ggattgcggt gctggcaact gcggaagctg cgctgcgtat
    30781 ttcacgcggc acgaaaacca ccttcagtta ttgggggatg gtggcggctc acctggggct
    30841 ggcagtgaca attgttggca ttgcctttag ccagaattat agcgttgagc gtgatgtgcg
    30901 catgaagtcc ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa
    30961 agaggtgact ggcccgaact ggcgcggcgg tgtggcgact attggcgtaa cgcgagaggg
    31021 taaaccggaa acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat
    31081 gaccgaagcg gcaattgacg gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga
    31141 gctggaaaac ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg
    31201 ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg
    31261 taagcgcgtg agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta
    31321 attccgttga ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat
    31381 gccgaagggg atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag
    31441 tttcgtctcg aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag
    31501 ggcaaaccag tactgcttaa cgtctgggcg acttggtgtc cgacctgccg tgcggaacat
    31561 caatatctga atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac
    31621 gatcgccaga aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg
    31681 tttgatggcg acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaaccttc
    31741 cttattgacg gcaacggtat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc
    31801 tgggaagaaa agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt
    31861 ttttattggg cgtgctgatg ctgatgatct ccggctcagc gctggcgacc atcgacgtgt
    31921 tgcagtttaa agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct
    31981 gcccgaaatg ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc
    32041 gtcagaaagt gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata
    32101 tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc
    32161 tgctgtgggt gctgccagta gtggctattg gcattggcgg ttgggtcata tacgcccgct
    32221 cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga acaaagcgtg ccggaaggta
    32281 agcgtgccgg atatgttgtt tatctgccgg gtattgtggt ggcgttaatt gtggctggcg
    32341 tcagctacta ccagactggc aattatcagc aggtgaaaat ctggcagcag gccacggcac
    32401 aggctccggc gttgctggac agggcgctgg acccgaaagc cgatccgctc aacgaagaag
    32461 agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag
    32521 aaggctggat tatgttgggc cgcgttggca tggcgctggg taacgccagt atcgccaccg
    32581 atgcatacgc tactgcgtat cgcctcgatc cgaagaacag cgatgccgca ctgggatacg
    32641 ctgaagcgtt gactcgttca tctgatccca acgacaaccg cctcggcggt gaactgctac
    32701 gtcagctggt gagaacggac catagcaata tccgtgtgct aagcatgtat gcgtttaatg
    32761 cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct
    32821 tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac
    32881 atttgtcgcc gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctagac
    32941 tgatgacaaa gtgcgcttag tttatgccgg atgcggcgtg aacgccttat ccggcctaca
    33001 aaattgtgta aattcaataa attgatcgat ccacgtaggc ctgataagcg tagcgcatca
    33061 ggcagttttg cgtttgtcat aaatctcagc ccgatgataa atccatcggg ctatttttat
    33121 tgtgccccgc gttgttgcag gaacagaatg gtggccgcca cgcgtgagcg gacattgagt
    33181 ttacgcagca gattgcgaat atgtactttt actgtctgct cggaaatatt caacaccgat
    33241 gcaatctgtt tatttgacag cccctgtgcc agctcgtgca gaacatccag ttcgcgctcc
    33301 gtcagcacgc tgaagggatc ttcttccgcg ccaaacattt cacgttcacg taagtactga
    33361 ttgacgcgtt cgctaaagac tttgctgcct ttcgctccgg tacgaatcgc ttccagcaat
    33421 acttccgggt cgctgtcttt caacagataa ccgtctgcgc ctgcgtctat cagcgcaaag
    33481 acatcgctgg aggcatcgga tacggtcagg ataataattt gcgcggtaac gccatccctg
    33541 cgcaaggcat tgagggtatc caggccactc atacctttca tattgagatc cagcaagatc
    33601 acgtcgatat ccagtctatt cgccagatcg atcgcgctcg cgccttcgcc cgcttcggcg
    33661 accacttcaa agccaggatc aagctccagt aactgacgaa caccgcgtcg cataagtgga
    33721 tgatcatcca caatcatcac ctgaaaaggc gttgcttcag gcatagtagt ctcctgaggt
    33781 tttattagaa tcattatgtt tgagcgtagc gttggtaacg ctaccaggcg cgtgaatgtt
    33841 accccatttt atgtatggac atgtagtgaa aactacaacc ttgtcgttcg ctttggcatc
    33901 atttttatta taaagacgga attttgcatc agcttctcat caagaaaaac aactaaaaaa
    33961 tcaatcacaa tacttgtgca tttctttaaa cagtttttaa aaactgactc cgggtatgga
    34021 gctatgggta ttttctgtac ccaatgcttt taactgcaat taatttcata ggatgaaagc
    34081 tcaatgcatc aatctggttc tgtttctctt tgtcgttccg caatatctgt tctggtggct
    34141 acagcgttat attcacccat agcattggca tcaacagttg agtatggtga gacagttgat
    34201 ggtgttatcc tggaaaaaga tatccagctg gtttatggga ccgccaataa tacgaaaatc
    34261 aatcctggcg gagaacagca tataaaagaa tttggtgtaa gtagtaatac tgaaattaac
    34321 ggagggtatc aatacattga aatgaatggt accgcagaat actcagtatt aaatgatggt
    34381 tatcaaattg ttcaaatggg tggcgcggca aaccagacta ctctcaataa tggtgtgcta
    34441 caggtttatg gcccagcgaa tgataccacg attaaaggcg ggcgcttaat cgttgaaaaa
    34501 gatgggggga ccgtttttgc cgctatcgaa aagggaggac tactggaggt taaagagggg
    34561 ggatttgcgc ttgcggtaga tcagaaagca ggcggtgcta ttaaaacaac cacgcgggcc
    34621 atggaggtat tcggaacaaa ccgtctcggt cagttcgata tcaagaatgg tattgctaat
    34681 aatatgttgt tggaaaacgg cggaagtttg cgagttgaag aaaatgactt cgcttataat
    34741 actactgtgg atagtggcgg cttactggag gttatggatg gcgggactgc aactggcgtt
    34801 gataaaaaag caggcgggaa attaattgtc tcaacgaatg cgctggaagt gagtggtaca
    34861 aacagtaaag gccaatttag tataaaggat ggtgtgtcaa aaaattatga actggatgat
    34921 ggttccgggc tcattgttat ggaggacacg caggccattg acactatcct tgatgagcat
    34981 gccactatgc aatcgctggg aaaggatact ggtacgaaag tgcaggcaaa tgcggtatat
    35041 gatctcggtc gatcagacca gaatggaagt atcacgtatt cctcaaaagc catctctgaa
    35101 aatatggtaa tcaacaatgg ccgcgctaat gtctgggctg gcacaatggt taacgtgtca
    35161 gtcagaggga atgatggcat tcttgaggtc atgaagccgc aaataaatta tgcacccgct
    35221 atgttggtgg gtaaggtagt ggtttctgag ggcgcttctt ttagaacgca tggtgccgtg
    35281 gataccagca aagcggacgt ttcgctcgaa aatagcgcat ggaccatcat tgccgatatc
    35341 actacgacga accaaaacac tcgcctcaac ttagccaacc ttgcgatgtc cggcgcaaat
    35401 gtgattatga tggctgagcc agtgactcgt tcatctgtga cggcaagtgc ggaaaatttc
    35461 attacgttga ccaccaatac cctgtcggga aacggcaatt tttatatgcg taccgatatg
    35521 gccaatcatc agagcgatca gctcaacgtc accggtcagg caacaggtga tttcaaaata
    35581 ttcgtgacgg acaccggtgc cagcccggca gcaggagata gccttacact ggtaacaacg
    35641 ggcggcggtg atgctgcatt tacgttgggc aatgccggag gcgttgttga tatcggtacg
    35701 tatgaatata ccttgctgga taatggcaac catagctgga gcctcgcaga gaatcgcgcg
    35761 caaattaccc cttcaaccac tgatgtgctg aacatggcag ccgcacaacc gctggtattt
    35821 gatgcagaac tggacaccgt gcgtgagcgt cttggtagcg taaaaggcgt tagttacgat
    35881 acggtgatgt ggagttcggc aattaacacc cgcaacaacg tgaccactga tgcgggagct
    35941 ggttttgagc aaacattgac gggcctgacg ctcggtatcg atagccgttt ctcccgtgaa
    36001 gaaagcagta caattcgcgg cttgttcttt ggttactctc attctgatat tggttttgat
    36061 cgcggcggaa aaggtaatat cgatagctat accctggggg cttatgccgg ttgggagcat
    36121 cagaacggtg cctatgttga tggggtggtg aaagttgacc gttttgccaa caccatccat
    36181 ggcaagatga gtaatggggc aacagcgttt ggcgattaca atagtaacgg cgcgggtgct
    36241 catgttgaga gcgggttccg ttgggttgac ggattgtgga gtgttagacc ctatctggcc
    36301 tttaccggct ttaccacaga tggtcaggac tacacgttat caaacggcat gcgcgcggat
    36361 gtgggaaata cccggatatt acgcgctgaa gcgggaacgg cggtaagcta tcacatggac
    36421 ctgcaaaacg gtacgacgct ggaaccctgg ctgaaagccg ccgtgcgtca ggaatacgcc
    36481 gattctaacc aggtgaaagt taatgacgat ggcaaattta ataatgatgt ggctggaacc
    36541 agtggcgttt atcaggctgg gataaggtca tcgtttaccc cgacgttaag cggtcatttg
    36601 tcagtcagct atggcaatgg cgcaggggta gaatcgccgt ggaatactca ggcgggtgtg
    36661 gtctggacgt tctgataaca gaaaataaac aggctgtgat gtgtcacggt ctgtttatcg
    36721 aattaattgc agatataaaa aaaccaaccg taagggttgg ttttttcttg ggatttttgg
    36781 cacgagagta gtttaattga gcctgtaact gcatgatttt aaatatgtag attaattgag
    36841 ttttgaaatc gtatatccaa acgtataact aaagtgattt gctgcaaaaa tgtacagctt
    36901 taaccgttat cattttccgg ctatccgcta tgaagctggc tggtggatga tcacggataa
    36961 cctgacatct acagccgcct taaaaagctg aacccgacaa gcatatcggc atcatgtcca
    37021 ccagcgtgac gcattttttt aattgcgcgg gcgtaaaaat ttgacggggc ggacagttcc
    37081 ataagtaatg gattgcagga atttagcccc cctcctgaat gcaattttcg tatatatata
    37141 tacaaaacta cctgtcccac ctgtcccacc tgtcccacta gcacttaata gattgatttt
    37201 gttagaaatg aattaggtaa ggtgggacaa gtccaggctg ttttgggaca agttgggggg
    37261 aagtgggaca agtcgagcaa gtgaatcaag ccgtgttagc gcggtaaacc gactgtgctg
    37321 ccgcaatttt gcgttgtcgg gagggtactg taacagccac catacgcgca caatcttcgg
    37381 tggtaacgca atccagacgt gcgcaacatc cccagacagg taaatcatta cctagcacag
    37441 ctaattgcgc cagaatcgat tacagcgcgt ttctgtggca ttcaagaacg gctatcacta
    37501 gtgtattgat atgtaatccg catatgagtt taagcaacaa aatgatccct tttcgaaaaa
    37561 ggcactaacc gttttttccg caatgatccg ttggtgattt tgtaacttat tgttttgtat
    37621 gtatgatttt aaatttggta gggttataaa taggggatgg ttttgtcaag aattgcaaac
    37681 tatgcttatt tattgctctt ggttcgtttg ttttctgttc gttaatgggg gatcattttt
    37741 cactttttgg gggatcattt cggagggggt ggggctggca acacgccagc accctcgatg
    37801 cgtttgtgtt tagcagtact cccagtctgt ggctagtaga ctctcaggag atagcgtaac
    37861 gctatcctct gtgacgggat tcaacgcaat caaatcgttt ccgcgccgtt cgagaaagac
    37921 gatgtacgga agatgccagg cactgcggcg agctggcacg cgtcggcctg gctgcaactt
    37981 gtcgataata tccaccagcg aaacgttcat gtgattcaaa aaactcctga aacgctctac
    38041 aactcaataa cacaaaaaat aatgcagaac tgccactata aaaccttgtc tgtctggtag
    38101 ttccgccaga cagattttgc gatttctatc cccatatccg cctgtgtgcg gtttgctgcc
    38161 tctctcaagc ccccgaaatt atccagtatg acgggcgaac gtgctgcctt gtggatacat
    38221 tccgcgcgac gttttactgc ctgttcgcgc tccttatcgg tgttcacctg ttgcatgacc
    38281 tcagcccacc gggccgccgc tctccggtac aggcctttag actccagatc ttccgcttta
    38341 ctgtcgtgaa tcatactcat acgccagctt gctctttgtt cgacttacgt ttgcgcttct
    38401 ctttcagtgc catcagtcgt gcttccgcgt tctgctgttc ctgttgtgtc acctcgccgc
    38461 atggctggct attcagatcg taacgcacgc caccagccat taaggcacgg taataacgcg
    38521 gagaatgagc ataagatgct agcgctgcac gtaatgcccc tgacccaaaa gcctgcgccc
    38581 tgacggcaat atcctgtatc aggtcttcaa atatccctac cttaagcggc ttcggtgatt
    38641 ccaggctgaa taattcaggc catagttcaa taagacggtt agcgcgcctg cggtttttgc
    38701 cctggcgttt ggtcatatgc cgccatggtg tcgcccctgt gggcttctgt tggcggctct
    38761 gcggtgcgtt ctgattgccg ggtatcactt tatgcgctga tgtggtttta tcctgctgtg
    38821 tcgtctgtgt ctttttcttt ggtatgccgt aaattgaatc g
//
LOCUS       DADLCQ010000026        38775 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000026 DADLCQ010000000
VERSION     DADLCQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.026"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JQG95_001182"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JQG95_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY5887810.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..570
                     /gene="hisL"
                     /locus_tag="JQG95_001183"
     CDS             529..570
                     /gene="hisL"
                     /locus_tag="JQG95_001183"
                     /inference="COORDINATES: protein motif:HMM:NF019659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY5887811.1"
                     /translation="MTRVQFKHHHHPD"
     gene            716..1615
                     /gene="hisG"
                     /locus_tag="JQG95_001184"
     CDS             716..1615
                     /gene="hisG"
                     /locus_tag="JQG95_001184"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY5887812.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1621..2925
                     /gene="hisD"
                     /locus_tag="JQG95_001185"
     CDS             1621..2925
                     /gene="hisD"
                     /locus_tag="JQG95_001185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY5887813.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2922..3992
                     /gene="hisC"
                     /locus_tag="JQG95_001186"
     CDS             2922..3992
                     /gene="hisC"
                     /locus_tag="JQG95_001186"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY5887814.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3992..5059
                     /gene="hisB"
                     /locus_tag="JQG95_001187"
     CDS             3992..5059
                     /gene="hisB"
                     /locus_tag="JQG95_001187"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY5887815.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5059..5649
                     /gene="hisH"
                     /locus_tag="JQG95_001188"
     CDS             5059..5649
                     /gene="hisH"
                     /locus_tag="JQG95_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY5887816.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5649..6386
                     /gene="hisA"
                     /locus_tag="JQG95_001189"
     CDS             5649..6386
                     /gene="hisA"
                     /locus_tag="JQG95_001189"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY5887817.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6368..7144
                     /gene="hisF"
                     /locus_tag="JQG95_001190"
     CDS             6368..7144
                     /gene="hisF"
                     /locus_tag="JQG95_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY5887818.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7138..7749
                     /locus_tag="JQG95_001191"
     CDS             7138..7749
                     /locus_tag="JQG95_001191"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY5887819.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="JQG95_001192"
     CDS             complement(7935..8912)
                     /gene="wzzB"
                     /locus_tag="JQG95_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY5887820.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="JQG95_001193"
                     /pseudo
     CDS             complement(9058..10178)
                     /gene="ugd"
                     /locus_tag="JQG95_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="JQG95_001194"
     CDS             complement(10428..11834)
                     /gene="gndA"
                     /locus_tag="JQG95_001194"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY5887821.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11943..12302)
                     /locus_tag="JQG95_001195"
     CDS             complement(11943..12302)
                     /locus_tag="JQG95_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887822.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12299..12850)
                     /locus_tag="JQG95_001196"
     CDS             complement(12299..12850)
                     /locus_tag="JQG95_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887823.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            complement(12951..13550)
                     /locus_tag="JQG95_001197"
     CDS             complement(12951..13550)
                     /locus_tag="JQG95_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887824.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13721..14659)
                     /locus_tag="JQG95_001198"
                     /pseudo
     CDS             complement(13721..14659)
                     /locus_tag="JQG95_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14722..15096)
                     /locus_tag="JQG95_001199"
     CDS             complement(14722..15096)
                     /locus_tag="JQG95_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY5887825.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="JQG95_001200"
     CDS             complement(15100..16248)
                     /gene="wzy"
                     /locus_tag="JQG95_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY5887826.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16268..17137)
                     /locus_tag="JQG95_001201"
     CDS             complement(16268..17137)
                     /locus_tag="JQG95_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HAY5887827.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17166..18056)
                     /locus_tag="JQG95_001202"
     CDS             complement(17166..18056)
                     /locus_tag="JQG95_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY5887828.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="JQG95_001203"
     CDS             complement(18086..19342)
                     /gene="wzx"
                     /locus_tag="JQG95_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY5887829.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGIYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYKINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="JQG95_001204"
     CDS             complement(19358..19903)
                     /gene="rfbC"
                     /locus_tag="JQG95_001204"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY5887830.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="JQG95_001205"
     CDS             complement(19908..20786)
                     /gene="rfbA"
                     /locus_tag="JQG95_001205"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY5887831.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="JQG95_001206"
     CDS             complement(20845..21744)
                     /gene="rfbD"
                     /locus_tag="JQG95_001206"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY5887832.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="JQG95_001207"
     CDS             complement(21744..22829)
                     /gene="rfbB"
                     /locus_tag="JQG95_001207"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY5887833.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGNQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23202..24095)
                     /gene="galF"
                     /locus_tag="JQG95_001208"
     CDS             complement(23202..24095)
                     /gene="galF"
                     /locus_tag="JQG95_001208"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY5887834.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="JQG95_001209"
     CDS             complement(24270..25664)
                     /gene="wcaM"
                     /locus_tag="JQG95_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY5887835.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRCSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="JQG95_001210"
     CDS             complement(25675..26895)
                     /gene="wcaL"
                     /locus_tag="JQG95_001210"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY5887836.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="JQG95_001211"
     CDS             complement(26892..28172)
                     /gene="wcaK"
                     /locus_tag="JQG95_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY5887837.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="JQG95_001212"
     CDS             complement(28352..29830)
                     /gene="wzxC"
                     /locus_tag="JQG95_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY5887838.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="JQG95_001213"
     CDS             complement(29832..31226)
                     /gene="wcaJ"
                     /locus_tag="JQG95_001213"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY5887839.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVVGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="JQG95_001214"
     CDS             complement(31281..32651)
                     /gene="cpsG"
                     /locus_tag="JQG95_001214"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY5887840.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWCFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="JQG95_001215"
     CDS             complement(32844..34280)
                     /gene="cpsB"
                     /locus_tag="JQG95_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY5887841.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="JQG95_001216"
     CDS             complement(34283..35506)
                     /gene="wcaI"
                     /locus_tag="JQG95_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY5887842.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQSGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35503..35982)
                     /locus_tag="JQG95_001217"
     CDS             complement(35503..35982)
                     /locus_tag="JQG95_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY5887843.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="JQG95_001218"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /pseudo
     CDS             complement(35982..36940)
                     /gene="fcl"
                     /locus_tag="JQG95_001218"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
     gene            complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="JQG95_001219"
     CDS             complement(36943..38064)
                     /gene="gmd"
                     /locus_tag="JQG95_001219"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY5887844.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="JQG95_001220"
     CDS             complement(38090..38638)
                     /gene="wcaF"
                     /locus_tag="JQG95_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY5887845.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38654..>38775)
                     /locus_tag="JQG95_001221"
     CDS             complement(38654..>38775)
                     /locus_tag="JQG95_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY5887846.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10357 a         9708 c         8723 g         9987 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgaat tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcctgactag tctttcaggc gatgtgtgct ggaagacatt
      601 cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac cttccggggg
      661 cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata agaaaatgac
      721 agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg atgactcacg
      781 cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc tgatcgcgat
      841 ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc cctgtctggt
      901 aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag aagagctgct
      961 taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg
     1021 cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc cgctctcctt
     1081 aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc tcgaccagaa
     1141 aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg
     1201 gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag ctaacggcct
     1261 gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg atggcgaaat
     1321 ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg gtgtgatcca
     1381 ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat
     1441 cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg atcaacagcg
     1501 cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa
     1561 agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg agtgatcgcc
     1621 atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca acgccagctg
     1681 ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa cgatattctc
     1741 gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa
     1801 accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg
     1861 agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac cttccacact
     1921 gcgcaaaaac tgccgccggt agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc
     1981 acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc gctcttctca
     2041 acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc
     2101 tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac
     2161 gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac ggaatctgtg
     2221 ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc aaaacgccag
     2281 gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg
     2341 gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct ttctcaggct
     2401 gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc
     2461 gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac cacccgccag
     2521 gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt ggagatctcc
     2581 aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga actggtcgat
     2641 ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc ggcaggcgac
     2701 tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac ctgttccagc
     2761 ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa agaaggtttc
     2821 tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac cgcccacaaa
     2881 aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc gtgactatta
     2941 ctgatttagc gcgtgaaaac gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg
     3001 gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg gagtttaagc
     3061 ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg attgaaaatt
     3121 acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggc gcggacgaag
     3181 gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc ctctactgcc
     3241 cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag
     3301 tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag ctggacggcg
     3361 taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc aatccgcagg
     3421 attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt
     3481 atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat ccgcacctgg
     3541 cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc ggatttacgc
     3601 tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac ccgctctcga
     3661 cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc gccatgagcg
     3721 aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg aaagagatcc
     3781 cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc tttaaagcct
     3841 ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat cagaataaac
     3901 aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg
     3961 tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt ttatcgatcg
     4021 cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt ttgacaaact
     4081 cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagcgg gctacaagct
     4141 ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag cggattttga
     4201 tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt
     4261 gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga aagtaaaact
     4321 ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg taattggcga
     4381 tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac gctacgaccg
     4441 cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc gttacgccca
     4501 tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg atcgcgaagg
     4561 tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc agatcgccac
     4621 ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg acgatcacca
     4681 caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa
     4741 acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg cccgctgcgc
     4801 actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct accagcgtgt
     4861 gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt acaccatggg
     4921 cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt
     4981 caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc
     5041 gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc caacctgaac
     5101 tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg tgacccggac
     5161 attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca agcagcgatg
     5221 gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca accggtgctg
     5281 ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa tggcgtcgac
     5341 ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct gccactgcca
     5401 catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca ggggattgaa
     5461 gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc
     5521 gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa cttctacggc
     5581 gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa aaacttcctg
     5641 gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg cgtctccatc
     5701 agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt
     5761 acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca aaagatccgg
     5821 ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg
     5881 ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct ggcgttgcgc
     5941 gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc tggtttgaac
     6001 gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag caaggcaaca
     6061 agcaagtggc agtcagcggc tggcaagaga actcgggcgt ttcactggaa caactggtgg
     6121 aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca
     6181 cgctggcggg atctaacgtc tctttatatg aagaagtgtg tgccagatat ccgcaagtgg
     6241 cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta cgtggcactg
     6301 gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc gtgaaggagg
     6361 ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt
     6421 gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac tggcaaaacg
     6481 ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt ccagcgatgg
     6541 ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg
     6601 tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct ttggcgcgga
     6661 taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc gcctagccga
     6721 tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg
     6781 taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca ctcaatggga
     6841 aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat
     6901 gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga
     6961 agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac acttcctgga
     7021 agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc acaaacaaat
     7081 aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga tcaggatatg
     7141 ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct gatgccggtg
     7201 attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc
     7261 ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa acagcgactg
     7321 tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc cccggactgc
     7381 gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca caaaggcacc
     7441 agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact ggaacaactg
     7501 ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact gtatgccagc
     7561 ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc actggcagca
     7621 acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta tcacctgctg
     7681 gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct gcataaacgg
     7741 catcagtgag ttacgggctg agcaggtgtg atgttgttgt cacacccgga gccattgccc
     7801 gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg ataagatgcg
     7861 ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc gtttctctta
     7921 aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc caacacaatc
     7981 cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc ctttttcgga
     8041 ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg aatatcaaga
     8101 tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata gtagtttggt
     8161 gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc ttcgctccct
     8221 agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc
     8281 tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag atctttctgc
     8341 tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg tcccagagca
     8401 atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac ttgctgaatg
     8461 tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga aacagtcaat
     8521 ggtaattgct ggttcttaac agaaggttcg atggtaagtt tttctggctc ttcctgatta
     8581 tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa cgtctcctgc
     8641 aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt gttatagcca
     8701 gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt ctccttcgct
     8761 actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat aattgtcatc
     8821 ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg gtcatggttt
     8881 tgcccagaaa cattattatt ttctactctc atcattaccc taactgacgg aaatttccaa
     8941 gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata aaaataatga
     9001 attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa caagatgtta
     9061 gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa gctcttctgc
     9121 catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat cacgttccag
     9181 gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca cttcaacgcc
     9241 tttcgccttg atacgcttca taatcccctg aatagaagac gcacggaagt tatctgaccc
     9301 gctcttcata atcagacgat aaatacccac cacttgcggt ttgcatcgac aattgccgag
     9361 atcaggttat tcggcacaga ctggtagttc gccagtaact gcttggtatc tttcggcaga
     9421 caataaccac cataaccaaa cgacggattg ttgtaatggt tgccaatacg cggatcgaga
     9481 caaacgcctt cgataatctg gcgagtattc agacctaaac tttctgcata actatccagt
     9541 tcattaaagt atgctacgcg catcgccaga taggtattag cgaaaagttt aatcgcttct
     9601 gcttcagtgg agtcggtaaa cagggtcggg atattttgct taatcgcgcc ttcctgtaac
     9661 aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga tgacaatacg tgacggatgg
     9721 agattatcgt aaagggcttt accttcgcgg aggaattccg gagagaaaat aatattttca
     9781 gtacgatatt tcttatgcat cgctgcggta aaaccaacgg gaaccgttga tttgatgacc
     9841 ataaccgcat aaggatttat ctcaactacg tctttaatta ctgattctac actggatgta
     9901 ttgaaataat tagttttagg atcatagtcg gttggagtgg cgatgatgac ataatcagca
     9961 tcccggtagg cttcattttt atctaatgtg gcattaaagt gtattttatc tgattgcaaa
    10021 aactgctgaa tttccttatc aacaatagga gatatccgat cattcagcat agcaacgcgt
    10081 gacggtaaaa tatctaatgc cacaacctca tgattttgtg cgattagaag cccgtttgac
    10141 aagcctacat agccagtacc ggaaatggtg attttcattt catgctctca gaattaactt
    10201 aactgtgaat catgatgttt ttagcatcgt gataagatgc taaaagtttt aacgctacgt
    10261 tgtcagatgg tcaacgcaac gaaaaacaat tcagattttc tttataagaa tagctgatgt
    10321 ttattaagtt aatattaagc agtgaaattt agtctataag atatttggca aaaaaaagcc
    10381 cggtgcaata tacgccgggc ctcaatttta ttgttggtta aatcagatta atccagccat
    10441 tcagtatgga acacaccttc tttatcaatg cgcttataag tatgcgcccc gaaatagtca
    10501 cgctgtgcct ggatcaggtt cgcaggcaga accgcggcac ggtagctatc gtaataggca
    10561 accgcagcgg cgaaggtcgg aaccgggata ccgttctgta ctgcataagc gacgacatcg
    10621 cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt acggggccag cagcaggtta
    10681 gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg cgcacggatg
    10741 atgcagccag cacggaaaat cttcgcgatt tcgccgtagt tcagatccca attgtattct
    10801 tcagacgcag cacgcagctg agagaagccc tgagcgtaag aaacgatttt gcccagatac
    10861 agcgcactgc gaactttttc gatgaactca gccttgtcgc ctgctgactg agcttgcgga
    10921 ccagagagaa ctttagatgc ggcaacgcgc tgatctttca gagaagagat ataacgtgca
    10981 aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct ctggctggtc
    11041 catttaccgg tgcctttgtt tgctgcttca tccagaatca catcaaccag gtagttaccg
    11101 tcttcatctt ttttggtgaa gatatcttta gtgatgtcga tcaggtagct gctaagttca
    11161 ccgttattcc actcggtgaa agtctgggcc agttcttcat tagatagatt caggccacct
    11221 ttgagcagag aatacgcttc agcaatgagc tgcatatctc cgtattcaat accattgtga
    11281 accatcttca cataatggcc tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg
    11341 tcttctgcca cggcagcgat tttagtcagg ataggcgcaa ccagctcata agcctctttc
    11401 tgcccgccag gcataataga aggacctttc agcgcgccct cttcaccgcc ggaaacaccg
    11461 gtaccgatga agttaaagcc ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg
    11521 aagaaggtat taccaccatc aatgatgatg tcacctttat cgaggtatgg tttcagggaa
    11581 tcaatcgcag catccgtgcc tgcacctgct ttcaccatta acaggatgcg acgaggcgtt
    11641 tccagagatt caacaaactc tttcaccgta tagtaaggag ccagtttctt gcctggattt
    11701 tcagcaatca cttcttccgt cttttcgcgg gagcggttga aaatagagac ggtataacca
    11761 cgactttcga tgttgagcgc aaggttgcgc cccatcactg ccataccgac tacgccgatc
    11821 tgttgctttg acattgttta ctcctgtctt ctatattatt aaccgtctat taaccgttat
    11881 attattaacc gtcgtgattt tcatcttcta atcaaggaat acatatggtt acagcatgct
    11941 tcttacttta tagcgataag tatgccattg ggtgtatcca taagaacttt gaattttgga
    12001 tacttttctg gattaaaatt agcatagctt cgtttagata taacaataaa cgaactatta
    12061 aaagatttca tcttctctat taaatcatcc tgtgggatta cctcacactc gggattacta
    12121 atagtccaat cactatatct tgctaatcca aagacgtcac caattgccat ccctttatag
    12181 aagtaaatac catcctccaa gcctaattgg ataagattat ctccatataa ctcactatat
    12241 tcacttgctt tcttcatcag ttcatagcta tagcgacttt ctaagcgtga attaaaattc
    12301 aagtattcag aataaaaata tttgaattca aaatacaacg atcgccccga aaaaaaataa
    12361 tacacacaag gataaaatat cgtcccaact tgtaatttgt aatgttgata tcttttatta
    12421 aacttgcaat tgagatcgca acctgcaata ctgacaatgg gtaaatgggt gataaatacc
    12481 tgtcaccttg cgtaacagaa aaccaaaata agacatatgt taaaacaatc gagaaaaata
    12541 cccaaagtat tttattattc ctaagtgtaa agacagacag tatagcataa aaaacaacat
    12601 cactgccaac tttattaatt gcttgttgca aatctaatgt ggcaggactt ataccatgtc
    12661 gagcttgttc tgctgtttga acttgtagat caatttcatt ccataggtag tgaccaaata
    12721 tatttcctcc agcaggacta aaaggatcac cagagatcca aaaactacgg acataccaat
    12781 aaatgccacc aagaattgtt gccaagctaa aaataagaaa tgtctgtatg tttctctttt
    12841 tcgctatcaa tataacaaat atgatggctc catatatggc accaaatagt tttacgccac
    12901 aagcaattcc aataaaaaaa ccacataaaa aaattaattc attttgtgtt ttattgtttt
    12961 ctgagcgaat atatataaga atactggctg cagcaaaaca aagatagcgt taaatcaata
    13021 tatgcataac caagatgatc tttaagtgga ttgatagtaa atagtaggat tgtagcaata
    13081 atgcctggat aataagtttt cgtaatttcc catgaacagc caacaagtcc acaggctatt
    13141 acaaaaaaac agatatttgc taaaaactga gctaacctta catctcctaa ctgtaaacct
    13201 aaagccatta aaagatctgc attttgcggg aacagaggaa aacgcaaata ttgttcaaca
    13261 acaattgcat ggtgctcaac taatgaacga gccaaaggta agtgatacat agtatcatcc
    13321 caatcaccgg gagctcgcag ggacgataac aatgtaacca caaaaatcaa aacaagaaat
    13381 aaaataacaa acagcgtact gaactcaata ttatttattg ccagccatct aaattgctga
    13441 cgtttaataa tgcttaaaac ccaacacacc gtaaaaatta aaaagatagc aatcttatta
    13501 aattgcccga ccatgcccag aaagtaaatt atcaaaatat ttaaagacat ccctatgcat
    13561 aggcaataaa aaataaagaa aatttgttaa gtcctgccac cccctcctta aaaaaagatg
    13621 acaatacaat attaccaatg acaaaaaatg tcattccaaa agcaaaacaa tacacaacat
    13681 acaaatacaa cggcaattca atcatagtaa aatactctat ttacattatc agtttatagt
    13741 ttttttcaga aagataaaat aatgccttca tttcacgtct agttgttgta tttgaatgca
    13801 aaataatgcc acatacaaat gccattattg acgataacat gatggaggca gttaaaaagg
    13861 cagtcggaaa tcttctcacc aaacctgtat caatataatc aacaatgatg ggcaatccta
    13921 aaattataga caaagcagca agtaaaacgc cgataatact aaaaaagtat agtggccgtt
    13981 caagcgagta aagtttaatt attgttttca gaatcttgaa cccatcactc caagttgata
    14041 gtttgctcac actcccttca gatcgctcgc catattttgt attaacctca ccatacttca
    14101 tacgtaattc taaagcatga attgtaagct cagtttcgat ttcaaatccc cttgatagac
    14161 atggaaaaga tttaacatag cgtctggaaa atacacgata ccccgacaac atatcagtaa
    14221 aattcccttt aaagatagtc tgaacagtac cagtcaacag tttatttcct cagcgatgcc
    14281 cttttctgta tgtattttga tctccattct cttgacgaca tcctacaacc atatccaatt
    14341 gattttttat gagatggtta atcatttctg gagcagaact tgcatcataa gtgtcatctc
    14401 catcaaccat tagataaata tccgcatcta catctgagaa catacgtctt acgacattcc
    14461 ccttgccttt cagaggaaca gagtgaactg ttgctccagc atcctctgcc acttgtgctg
    14521 tactgtcagt cgaattatta tcaaatacat atatagaagc agttggaata ttcgttttga
    14581 aatcatttat gacttttgct attgccttct gctcattata acaaggaata attacagcga
    14641 tattaaaaga gtgatacata agcccctcat ataaactatt tagctaatgt gcaagtaatt
    14701 gtgtttttaa caaatcgttt actaacgaaa aatgaaatat tttgatgcgc aatatcctaa
    14761 aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt ggagataaag ctaagagatc
    14821 aaataaaacg ccgaataaat aactcatcgc ccccataaaa aatataaata taaaatatct
    14881 agccttggtt gcagatactt taaatgagca tcttgcattt gcaaaaaaag aaaaggttgc
    14941 tgcacatata aaaccaacaa tgttacttcg gccttgacca aaagctaaat tattataaca
    15001 cgcataaaat accccccaat gtaaagcagt attgacaatt ccaattaaaa aataactaaa
    15061 aaaagaactg gtcaataact tccctatttt taacatcctt tattttgctc cagaagtgag
    15121 gttattacta atttggatat tttctataga aaatacccat aaaactacaa cgcctataat
    15181 aaaaattccc atgggtgtaa aaagaaatgg attagttata gaaacggtaa tactgaaaaa
    15241 aagtattaac gacatcatta atgatgtttt tgtactattt aaaaaatagc ctttcaaaaa
    15301 gataagcaac ataggaacta atgatataaa tacaccaata acgcctgttt tctgaagtat
    15361 ctcaagaggt actatttcaa tccttaatcg atctaatata aatgaaccaa atcctcttcc
    15421 aaacaagaac gtcgctaaat caacattttt ataataaaaa tataaatcat taaatctaac
    15481 agagtcagaa tcttcactgc gtgatccgac catgtaaagc ataattataa atagaacgct
    15541 gcccaatatt attataatag tatttaaata tagcttatgt aatttaaaac tggctaataa
    15601 cagtgataat gttgtaaaaa gtaatacacc tcttgattct gtcaataata tcgcaagcac
    15661 tattatgaga cattttaaat attttttgtc gacaaaactg ataatcgcac cgacaccaaa
    15721 aaagtagaag gatttggaaa aaaaagcccc atcgggacga aaaaagaact cgctagtcaa
    15781 tgataagtgt tcataaatta aattaaaatt taacaacccg aaattcaaca aaattatgta
    15841 tgaaatatat aacagtgatc catataatgc aaatgcatta actattttaa taaaaatctc
    15901 acatgaaaat acttttaaag tatatcttag tgtaaataga tatataagtg acagcataaa
    15961 aaggtatggt tgcacatcaa acagtatatc atttatttta ttaccagcta ataagccgac
    16021 aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa agactgacac atttttgggt
    16081 gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt ataaaactaa atacaaataa
    16141 taaatatcgc aaagggaata ttcccaatgg ctccagtaat cttccaccac caccaataat
    16201 cagttcaata cataaaaatg ttataaaaat tttatttata ttattcatat tcgtaaggtg
    16261 atgtttttta aaatatgttt aaaatcaaca ttaataaaat ataaatttta tcagcctttt
    16321 tccgccttaa cttccaaaaa cttaaagcca agatcaaatt gcctatataa cctttaccca
    16381 attgattatt cacaaagcta tcatcagcta aggcattagc tattttagtt acttcttttc
    16441 gataccactt gtttctcacc aaaccaagcc gtttgtattt tgctttaaaa ctaatgtttg
    16501 ctcccacttg gttgttttca tgctgtctgt acagcattgt tggttttcta tcaatatacc
    16561 acgaataatt tctcgtccgc gcaaatgaat ataaaaacca atcatgcaga cagatatctt
    16621 gtgcagcatt tttattattt atgatgaact tcttaaattc aataagcgtt tcttttttta
    16681 taacatatgt acaccccgga cctgctgctt caaaaaaata atcgaattga gtttgcgact
    16741 gagccttatc aactaaattt tttcgccctg atggataata tgcaattaca ttgctggaat
    16801 aacaattcgc attattaaat accaagcagt caattgcatt ttttatttta tctttatacc
    16861 aaatatcgtc ctgatcacta aaacagatat aatcatagtt ttcaagatcg acatctttaa
    16921 ttaatctata aaaattttta cctgcccccc caaacttatt gacgcttgga agtatattta
    16981 taattgaggg atatgataat tgaaattctt cacaaatatt taatgtatta tcagttgata
    17041 aatcatctga tataaaaatt gagatgtcta catctttttg ttcaataata ctttttaatt
    17101 gttcctcaat ccagcattcg ccattatacg tagccataag tacagctact cttttttgat
    17161 cagaactatg cattataacg accgccccca gtaattcctc ttattccatc cagcgtagcc
    17221 aaaatatgtt ttttcatata ttcaatttta tttttttgag ttgcgataat taaagtcgta
    17281 tgaattaaag aaaaaacaac ttccctaata gagagtaaaa gaggcacatg attttttcta
    17341 agcaaaagaa atgcatttct tactcgataa taacgacgta ctggcgagtg aattggtatg
    17401 ttaatcccac agatttttac tgacttatcc ccaatagcat gaaccatttc tatatcagga
    17461 atgatatgaa ctaatatgcc atagtttaag catctaagac accattctgt atcgacataa
    17521 tcaataaaaa gttcctcatc catcattcca acaatctcaa aaacttcttt tgaaaccatg
    17581 gttccggatg agattgttac tgaactttta aacggtgtct gtccttcagt gacatgaatt
    17641 ttctctcgaa gaccattttt tttgatatta caaataggat aataatgtga cttatctcta
    17701 tcaataaaaa ctgggccagc tatttttgca ttttcattat ccatatagca ggaaagtttt
    17761 ttgataaact cattgcgaat acggctatct tgatcaaaaa aaattatctt ctctgcccct
    17821 ttttcaagag aatatcgaat cccaatattt tgtgcttttg ctataccttc attcctacca
    17881 agacaaatta aatgaacttt ttcaatgtct gctaatttat ctttcaaggt gaaattagtc
    17941 ctattatcga ctactacaac gtaacaattt tgctccttta gttcagtgat cagtgcattc
    18001 agatttttaa gttcgggatt atatgtcaca atgacagcgt aaatattact attcattatc
    18061 caagtgactc agtaattggt taattttagt tttcatatac agaacacatt ctagtataaa
    18121 gccattgtaa taacagagca tctaaaatta cacgtatgct ccaggatatt gcggcaccca
    18181 gtaacccata tttttcgacc ataaaataca aaagggctaa ataaggaacc aattcagcac
    18241 aatgaatcaa agcagtaatt ttagattttc ctaatgcctg gattgcagag aatggtatca
    18301 ttgccagcgc attgaagaaa aaaccgatca ataacacatt taatatatta aaggaattaa
    18361 ttttataatt ctcaccaaac catattttca atacaagccc tgaatataac aagcctatga
    18421 ttatcaccgg caaacagatt agaaacatta aaagcaatga tttatttaca ttacgtttaa
    18481 attctttaaa gtctttgatg ttacttaacc ttggaaacac tgccctcccg attgctgcag
    18541 gtattattcc taattttaga ataacctccg ctggtgcaga ataaaatgca actttatctg
    18601 cccccataat atttgacact ataaatcgat caaaatacac catgaccgga cttatgatat
    18661 tacttactgt catccaaccg ccaaagaaaa agagtcgttt aaaggtttta taacaaaaat
    18721 gaacccccga aattttaata tcattcctga cataataggc agagactaaa atcgaaataa
    18781 ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt agcactataa aaaacaaata
    18841 tggccggaat tcccgcgatg caagagcttg atatagattt ttgaatattt acaatgccaa
    18901 atttttcatc cccctcaaga atggctgacc ataattgatt tagaataaat aatggaatgc
    18961 aaatagccaa cagtttaaat gctagttgta tatcactatg ttcaacacca gaaattttta
    19021 gataattaac aattccatca gagaaaatca ataataaaaa agcaccaaag catgaaaata
    19081 gcaccaaaaa agatgtactt gttgaaatta ccttggttct ttcatgatga ttatcacgat
    19141 gaattgcaat ttctctaata acagagcgag tcaggcctac atcaaaaatt ccagcatacc
    19201 caacaagtgc aattgcaatt gtataaatac cgaaaccttc aggccccaaa cctcgagcga
    19261 gaaatcctag cgaggggata gcaattaaag ttggtattgc atagccaaaa aggttccaga
    19321 cactattttt tattatgctc attattacat ttctttatca tataaaataa tcagcatcta
    19381 caaatttttg accattaata tctttatcag aaagcaatgg attttgaacc ggccattcta
    19441 tatttatact tttgtcgctc cataaaatcc cacgctcttg catttttgaa tagaaattat
    19501 ttgttttgta gacaaattct gcttcatcac taagaacgca aaacccatga gcgaatcctt
    19561 ccggtatcca taattgacgc ttattctccg ccgacaaatt tacaccaacc cacttgccaa
    19621 atgttggtga tgatttccga atatcaacag ctacgtcaaa aacttcaccg acaacacagc
    19681 gtaccagttt tccttgagca taaggttcca gctgatagtg cagtccgcgt aacacaccct
    19741 tagttgattt agaatggtta tcctgaacaa actcaacctt tcgtcctaca gcctcttcga
    19801 aaactttctg attgaagctt tccataaaaa agccccgctc atcaccaaat acttttggtt
    19861 caaaaattag cacatcagga atttcagttt taattacgtt cattttatta ataaccttta
    19921 atcattttca gcagatactg accataagca tttttcttca gcggctcggc taataccttc
    19981 acttgctcag catcaataaa gcctttacgg taagcaattt cttccgggca ggaaactttc
    20041 agcccctgac gctcttcaat tgttgcaatg aagttgcttg cttcaataag actttgatgt
    20101 gtccccgtgt ccagccatgc ataaccacgc cccatcatag cgacagacaa acgtccttgg
    20161 tccatataaa tacggttaat atcggtaatt tccagttcgc cacgtgcaga tggtttaagg
    20221 tttttcgcca tttctacaac gtcattgtca tagaaataaa gcccagtaac cgcatagtta
    20281 ctttttggtt ccagcggttt ttcttccaga ctaattgccg taccgttatc atcaaattcc
    20341 accacaccgt agcgctcagg atcatttacg tgataggcaa aaaccgttgc gccactctct
    20401 ctgttaacag cggtatccat taatttcggc aggtcgtgac catagaagat attatcacca
    20461 agtaccagcg cacaatcatc accacctata aattcttcac caatgataaa tgcttgcgcc
    20521 agaccatccg gactcggttg tactttatat tgcagattaa gcccccactg gcttccatca
    20581 ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac tgataatcag aatatctcga
    20641 atacccgcca acataagcgt tgaaagcgga taataaatca tcggtttatc ataaatcggt
    20701 agcagttgtt tgctcacagc cattgtcaca ggataaagac gagtgccgga accaccagcc
    20761 agaataatac ctttacgcgt tttcatttca ccattccttt taattcatct cgctctagca
    20821 tcatgagcga gatgcaataa tttgttaaat tgccgtggtc gtaaataatt cgttgagcat
    20881 acgtttcacg cccacctgcc agtcaggcaa gacaagcgca aagttctgct gaaatttttc
    20941 ggtattgagg cgagaattat ggggacgacg tgctggtgtg ggataggccg ttgtcggcac
    21001 ggcattaagt ttgttaagag caaggttgat ccccgctctg cgcgcttctt caaataccag
    21061 agcggcataa tcgtgccagg ttgttgtacc accagcaacc agatggtaca aaccagcaac
    21121 ttctggttta tttgcggcca cgcgaatggc atgagcggtg caatcagcca gtaattcagc
    21181 ccccgttggt gcgccgaatt gatcgtttat tacagccaac tcttcgcgtt cttttgccag
    21241 gcgcaacatc gttttggcaa agttatttcc tttaccagcg tatacccagc tggtacggaa
    21301 aataagatgc tttgcgcaat gttcttgtaa cgctttttcc ccagctagct tggtttcacc
    21361 gtagacattt agcggtgctg ttgcatccgt ttccagccat ggcgtgtcac cgttgcctgg
    21421 gaaaacataa tcagtggagt agtgtataac ccaggctccg acttcattag ctgcttttgc
    21481 aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa ttcggttctg attccgcttt
    21541 gtctactgca gtgtgagcag cagcattaac aataacatcg gggcgaattt ttttgacggt
    21601 ttcagccaca ccttcggggt tgctgaaatc tccacaataa tcagtggagt gaacatcgag
    21661 cgcaatcaag ttacccagag gagccagagc acgctgcagt tcccaaccca cctgccctgt
    21721 tttgccgaaa agcaggatat tcattactga cggccctcat agttttgttc aatccacgac
    21781 tgataagcac cgcttttcac attctcaacc caatttgtat tagccagata ccattccacc
    21841 gttttacgaa tcccgctctc aaacgtttct tgcggtttcc agcccaattc gcggctaatt
    21901 ttatcggcat caatggcgta acggcggtca tgccccgggc gatcggcaac ataagtaatt
    21961 tgctcacgat aagatttctc tttcggtaca atctcatcca acaaatcaca aatagtgagc
    22021 actacgtcga tgtttttctt ttcgttatgt ccaccaatgt tataagtttc acccgcttta
    22081 ccttcggtta cgacggtata taacgcacga gcatgatcct ctacatacaa ccagtcgcga
    22141 atttgattcc ctttaccata aataggtaat gccttacctt ccagagcatt cagaataacc
    22201 aatggaataa gtttttccgg gaagtgataa ggaccataat tgttcgagca attagtcaca
    22261 atggttggta aaccataagt ccgcttccag gcacgtacca aatgatcgct ggaagcttta
    22321 gacgcagaat acgggctact tggcgcgtaa gctgtcgttt ccgtaaatag cggtaacgct
    22381 tcgttattat ttgcttcatc cggatggggt aagtcaccat aaacttcatc agtagaaata
    22441 tgatgaaagc ggaaactttt tttcttttca tcattcagag cagaccagta attgcgcgcc
    22501 gcttctaaaa gaacataagt ccccacaata ttggtttcaa taaatgccgc agggcccgtt
    22561 attgaacggt caacgtggct ctctgccgcc aggtgcatca ccgcgtctgg ctggtgctgt
    22621 gcgaaaatac gcgacatggc tacagcatcg cagatatctg catgctcaaa agcataacgt
    22681 tcagaatcag aaacatcagc gagtgattcc aggttcccgg cgtatgttaa tttatcgaca
    22741 ttaacaacac tatcttgcgt attatttatt atgtgacgaa caacagcaga accaataaac
    22801 cctgcgccac cagtaacaag tatcttcact tttttattcc atatagctat agagcatgct
    22861 gtgaaataga ctgctccaga tttgattaat agaggtatta atacacgcta ccgcccctgg
    22921 cttaacagct accagtgcac tgcatgcatc tcaacgatgt ggcgagcgtt agccactcgc
    22981 gccaaatccg aaaaattcaa acgttaattg tcttaccaaa cagccgtaga aacaaggaaa
    23041 atcctggaaa aatttgaata atatgagcta tgtaacttct tgtttttata ggctttataa
    23101 aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg ccacgaatct tcactgccgt
    23161 tataattttc ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc
    23221 tcaatacctt tacggaactt cgccccttct ttcaggttgc gtaggccata cttcacaaac
    23281 gcctgcatat agcccatttt tttgccgcag tcgtaactgt cgccggtcat cagcattgca
    23341 tcaacggatt gttttttcgc cagctcggca atagcatcag tcagctgaat acgtccccat
    23401 gcaccaggct gagtacgttc cagttccggc caaatatcgg cagaaagcac atagcgacct
    23461 acggccatga tgtctgagtc cagcgtctgc ggctgatccg gtttttcgat aaattcaaca
    23521 atgcggctga ctttaccctc acggtccagc ggctctttag tctggatgac ggagtattca
    23581 gagaggtcac ccggcatacg ttttgccagc acctggctgc ggcccgtttc gttgaaacgt
    23641 gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc tggcatcgtc gatcacaacg
    23701 tctggcagta ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag
    23761 tggcctaaac ctaaaggttc gccctgacgc acgttcataa tgatcacgcc cggcggacag
    23821 atggactgta cttccgccag cagttgacgc ttcacgcgct gctcaaggag tgattctaac
    23881 tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt taccaggagg
    23941 atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg
    24001 acgattggta gcatctcttt gggtatcgcc ttagtggcag gcaacatatg catcccgaga
    24061 cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg taaaatggtt
    24121 gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa ttgattactg
    24181 aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt cctaagtatg
    24241 gctccatttt tccaggaatg gtcgcaaatc tactccctcc gttccggcaa tctaaagtta
    24301 atcttttcca cattaacaat atggtgatta atcctgtcga tatcgacgga gctttgccct
    24361 ctttcattca ccgcatgaac atttgcaaga gacagcagtg tttctttttt cgccataaag
    24421 acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc aactgaggat
    24481 tcctgcatca cattgatatt tcgcataaaa agatgttgcg gcttgttgtg taactccagc
    24541 gaggcacgct tcatctcaat gttagttagc gacacaaagg agacggcatt cccggcggag
    24601 atttggatgc cgcgcaattt ataagcaaga tgggtattat ccagttgaat attattcact
    24661 cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc gattaacatc
    24721 ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc gtaaatagcg
    24781 actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt gcgggcattg
    24841 atattacgaa taacaaaatg tttaccattt tcaacatgga tcaactgccg acaatccgat
    24901 cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta gttgttatca
    24961 taagtgcttc ccgcaaggcc tatgccgatg ccccagttga ttttgccgtt ggtacagttg
    25021 atacgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc cacgttccat
    25081 tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg taatgttggc accgataatc
    25141 tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt gaggttatcg
    25201 atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa tataaatctg cattaccggg
    25261 ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag aacaacgcac atccagggta
    25321 atgttatgca taccgccgcc ctctccccct gtcacctggc tgccgtcctg taagacaaat
    25381 cgccctctgc cgttgccgcg caggcttcca aggatgtgta acgttttgcc gggagggatg
    25441 aagatgccgg tgttgatatt gtcacaaacg aatccggcag gcacgacgac cgtttgccct
    25501 tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg ggttgtagtc gttaatgtta
    25561 acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag
    25621 cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg gcatacgttc tcctctataa
    25681 agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat cgtgttcgac
    25741 tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtatccagtt ggctaaacgc
    25801 cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc agcagccagc cggatttatc
    25861 ggcctccacc agttcaggaa ttccgctatg cagagtagaa accaccggaa taccgaccgc
    25921 catcgcttcc attagcgcca ccggaatgcc ttccatatcg ccatccgcgc ccgttaccga
    25981 tggcaacagg aagacatccg cgtcgtcgag catctccttc acttcatggc tcggtttaaa
    26041 gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtgc gcaggcgtcg
    26101 ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct ctttcaactg
    26161 acggcaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg cgacagatat
    26221 gatttccagc ggcgttgcgg gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac
    26281 gcccatacgc gatacggcga ttttttctct ggggcagccc attttttgca gccttccggc
    26341 ccacagatcg cttatcggta acatcaggtc gccacggcga aacagttgtt gatattcggg
    26401 agtgtagtgg ttgagcactt cccgactgga gatatcaata ccgtggaaga tggtggcaat
    26461 tttgccgcga atgataccca gttcgcgtag ttttgctgcg gttacccccg ctgggccaaa
    26521 atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc tgaccacaaa tggcagacaa
    26581 aatcaggttc cgcgactcgg caccatagcg tttgaggttg agcgcatgcc aggtattttt
    26641 acgatgaatg ccacgtaagg tctggctggc gcggtggcgc agtttcgcta ctttgcccgc
    26701 aggttcgtcc tgtaaccagc gggttctggc gacaaggttg tatttcgtcc atgccgcgtg
    26761 ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc acttcaaagc ccatatcaat
    26821 aaacgcggta atttgattga ggacgaaggt ttccgacgac agcgggaatt tcaataagaa
    26881 gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac catctgcatt
    26941 cccgtctgac gttcgcgact gacggcttcg ttaaatcgag cgttcagcac cggaagctgg
    27001 cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg aatatcaatc
    27061 gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc atagttgatg
    27121 gcaatcgccg gagtagcaaa gttcattgag ataatggcgg agtgcaggcg cgtaccgacg
    27181 gtgagttcac aggctccgag aattttgccc atttccagat cgttgagttc atccatcact
    27241 acgtggtaac gggcaggatc gctgatgtgc tggcgcagat tgagcgccac catgcggtcg
    27301 tctttgttat agctgtcgat acccgtacag gtggagagcg caatcacctg atagccttca
    27361 tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg agtggtgccg
    27421 agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ccactgtttt ctgttgtgcg
    27481 gcaacgtcca gccagtgttg aacggcatag ctggcggtga agtcttcggt gtggtgatcg
    27541 accagccacg cggtatcgac gccatgttcc acttttgcgg tggtgatatt tctgcgtttc
    27601 atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc aaagacatag
    27661 ttcgccagtt ggttaaattg ctcatcctgg aaggggccga cgctgtgacc aatcataaac
    27721 agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac gccgtagaga
    27781 tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag caggcgcacg
    27841 aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc ggtgacgcgt
    27901 gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaatgcg cccgacaacg
    27961 cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg gatcgcccat taccgggcgg
    28021 ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc ggtatgtgga
    28081 ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg
    28141 caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg atgccaggtg
    28201 aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg cgcgtcttat
    28261 caggcctaca ggtcccgagc acagaaccgt aggacggata aggcgtttta cgccgcatcc
    28321 ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc ccgtaaaagc attttcattt
    28381 tttcgctgcg acaaaactga cgcttcactt ccaccaccag cggatggcgt gacagcacaa
    28441 tcatcaccac aaacgccagc acgcccgcgg ctatttgcac cgccagcagc atccccagcg
    28501 ccagttgccc tttcagcaca atgcccagcg cataactgac caccagcgtc ggcaacgaga
    28561 gataaaacgg cagccacaaa ctcaggatgt actggcgata actggaacca agaaccggtt
    28621 taatcatcac gaagtaactc agaatggtgt tgataatctg caccagcagg aaaccgagcg
    28681 tgacgccgat tgcgcccgcc atctgcccac caataacaat cgccggaata aacagaaatg
    28741 ttttgaatac gttgaattta aagctgatat cgacccgcgc tttcgccatc agcagcgaac
    28801 caatcgggtt acccacggag cgcagcagac ccacaataca cagcaattgc agcaccggaa
    28861 taatgctgtt ccacttttcg ccgaatacca gcggtacgaa gttattcgac accaccatca
    28921 gccccagcag cgccggaaag ttgataatcc ccactatcga cagcagcttg tagaagttaa
    28981 cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc cggaaacaac acgcgggtga
    29041 tgattgggtt cagcttcatc ggcggtacga cggccacgtt gtacgccagg ttgtatcccc
    29101 ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt tgaaaggttg gtgttgagat
    29161 agttgatgat gctgtccgcc gtcagccagg caccaaaacg taagttcggt gccacagacg
    29221 ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc aaagtagcca aacagcagtg
    29281 ttctcaccgc actattgacc agataaccga ggatcgcggt catcgccagc ggccagaaat
    29341 gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc gctggtttcg atcatgccga
    29401 ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa ctgttgcccg tgggggatca
    29461 ccacaaacgc cagcgacaat gttttaatca acggtgccaa gtccgggtta ttcagcacgt
    29521 cgccgatgac atcactcaac aaaaacaccg ccacacatac cacaagcccc agcccgacgt
    29581 tcagccagta cagcgtggtg agttcaaggt gactgatttc ttttcgctga ataatcgagt
    29641 tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat aatcaccagc gacacagtca
    29701 gcaggccgaa ctggtggttg tcgataatcc gcgccagcac ggtcatctgc accagcccga
    29761 ggccgatgat gatcaccgtg gcaatagccg accacttcgc gccgctgatg gttttttcac
    29821 gtaagctcat atcaatatgc cgctttgtta acgaagccct tgaataccgt caggaaaacg
    29881 attttgatat cgaaccagac gctccattcg cggatgtact caaggtcgaa ctcgacgcgt
    29941 ttttccattt tctccagcgt gtcggtttcg ccgcgccagc cattaatctg cgcccagccg
    30001 gtaatgcccg gtttcacttt atggcgcagc atgtagcctt caatgagctg gcgatactgt
    30061 tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg acatcccccc ggtcagcaca
    30121 ttgataaact gcggcaattc gtccagcgag gtgcggcgca gaaagttccc cactttagtg
    30181 acgcgcggat cgttctgcgt cgcctgggtc accactttgt cgttctccat cactttcatg
    30241 gaacggaact tccacacttt gatcggcttg ccatccatac catagcgagt ctggcggaaa
    30301 ataaccggcc ctggtgaact gagtttcacc gccagcgcaa tacagcacag caccggggag
    30361 atcagcagca gaataagcgt cgccagcaca atgtcttccg cacgttttag caggcggtta
    30421 acccccgaaa gcggcgtgtc atacagcggc accaccggaa cgccgttcat ctcttcgaga
    30481 cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca gcagcaccga acaggtggta
    30541 tccgccagtt gatggaccag ttttttcact cgtgcgccgt cgcacatttg catcgcgata
    30601 tagacgttat gaatcttgcc cgcttttgca tcctcgacca gctgttgcag gttacccgcc
    30661 cagtcgttag aaacgccgcc cggtttcggg tcgtggtaaa cgcccaccac ttcaaaccct
    30721 aaccacggct gattacggaa actctccatc agcatttgtc cggcggctaa atcccccact
    30781 acggcgacca tccgcttgtt gtagccatga ttacgcagcc agcccgcccc aatgcgaata
    30841 catgaacggc aaaccaccag tccgatgctg gtcagcccat accacgccag ccagattttc
    30901 agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg cgctgaaaat cacgcttaag
    30961 gtccagtttt gtagcaacag ggcaaattct gtcgctgccc gaacaccgcg ccatgagcga
    31021 taaaaatcgg tgatgccgcc cagcatctgg aacaccacca gcgtaatcag cgccaccaac
    31081 aggtgcatgt agaggaatga cagtccgctg acttcgcaaa ccagccatag cccggcaaac
    31141 atgatggtga tatctgaaaa gcgttgcacc atagagatta acgatgcatt ggttttcgct
    31201 cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta ttagcccctt acccgcagtg
    31261 gggtaaggga agatccgaca ttactcgttc agcaacgtca gcagagttcg cgttcgcgct
    31321 tccatcagcg gcacatcacc gcgcgattcc acattcaggc gcaccaccgg ttcggtattg
    31381 gaggtgcgca ggttaaagca ccagtcggca aaggtcatgc tgatgccatc gctgcgatcc
    31441 accgccagcg cctcacggct aaaatgctgt tccacgcggt taatcgcctc aacgggttgc
    31501 gccagtttgc tgttgatctc accgcttgcc ggaaacgccg ccatccggtc gcgcaccagt
    31561 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    31621 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc gccaccgtag
    31681 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacattacc
    31741 ggcgtgccgc ctgcagcagt caccacatca atagtgttcc aagagagacg cggatcatgg
    31801 atgatcttcg cgccgggatt tttttcgagg aacgcttctg ccagcaggcc gacaatgtag
    31861 tagccctcga taaactgccc tttttcgtca aacagaaaac agcggtcaaa atcgccatca
    31921 aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat tgcgggtgtc gtcgcgacat
    31981 tccggcagca gcgggttagg aataccgttg gggaaattgc cgtccggtgt gttgtgtact
    32041 ttgattaatt ccacgggcgc gccgagggct ttaaagcggg cttcaatggc gtccaccacc
    32101 ggacccgctg cgccattccc ggagttgatc accagcttga gcggcgtgag atttttgaca
    32161 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca ggttgatttg ctgatagcga
    32221 ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt cagccagacg ctggacgtcg
    32281 cgcagtccgg tatcaccgct gatcgtgcga gccccctcgc gcaccagctt catgccgttg
    32341 taatccatcg gattatggct ggcagtaact tcaatgccgc catccacgcc gagatggaac
    32401 gtggcgaaat agatctcttc ggtgccggac ataccgatat ccagcacatc gacgcccgca
    32461 tcctgtaaac cttttgccag cgccagtttt aaggtttcgc tggtgagacg gacatcaccg
    32521 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    32581 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    32641 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    32701 tttatttttg tcgttattta caccgcggtt tcgcattcat tgcctgatgc gacgctgccg
    32761 cgtcttatca ggcctacaaa accgaaccgt aggtcggata aggcgttcac gccgcatccg
    32821 acaaacagcg cctgatgcga cgtttacacc cgtccgtagc gatccgcgaa gcgcaccaca
    32881 tcatcctctt cgagataaga gccggagcgc acttcaatta aatcgagcgg aattttcccc
    32941 gggttttcca ggcagtgcgt cgcccccagc ggaatataaa tggactcgtt ttcaccaagc
    33001 agtttgatat caccatcaat ggtgactttt gccgttcccg cgaccactac ccagtgttcc
    33061 gcgcggtgat ggtgcatctg taccgacaaa ccctcgcccg gtttcacggt gatgcgtttc
    33121 acctggtagc ggtcgcccgc gtcgatagag tcatatttgc cccacggacg atacacttcg
    33181 cgatgcaccc gatgctcatg gcgaccatcg gctttgatct gctcgaccac ttttttcaca
    33241 tcctgtaccg cgttacggtc ggcaatcagc accgcatctt tggtctgcac cactaccaga
    33301 tctttcaccc cgacggtggt gaccaggcct gattcggcat acacatagct gttttcagtt
    33361 ttgtgattaa tcacatcgcc gtggcaaacg ttgccctcgg cggtgtgggc gctgatctcc
    33421 cataatgaag accaggaacc aacatcgctc cagcccgcat ccatcggcac cacaacagca
    33481 tctgccgtag gttccatgac cgcgtaatcc accgactctt ccgggcaggc gagaaacgct
    33541 tcttcatcca cacgaataaa atcgagatcc ggatcgacgg cgctcatcgc tttttcgcag
    33601 gcatcgagga tatccgggcg atattttttc agttcttcga gatagcgtcc ggcgcggaac
    33661 aggaacatgc cgctgttcca gtaatattcg ccgcttgcta cataggcctg ggcggtttcc
    33721 agattcggtt tttcgacaaa ctgcgccact tcaaaggcca ccgtatcctg ctcacccaca
    33781 gacacttcac cgcgacgaat atagccataa ccggtttctg gtagatccgg cacaatgcca
    33841 aaggtcacca gcttgcccgc ttcggcataa ggcatggcat tacgcacggc ggcacggaac
    33901 gcgtcttcat cggcaatcac atgatccgcc gccaatacca gcattaacgg gtcgctctcc
    33961 gggctatgac gttttgccgc cagcgccgcc agtgcaatgg caggtgcagt gttacgccct
    34021 gccggttcga gaataatgtt ctcggtgagt ttgttcagtt gacgcagctg ttccgcgaca
    34081 ataaagcggt gctgctcatt gcaaatcacc accgggcttt cgcactccac gccgttcagg
    34141 cggcagatgg tggtttgcag catggtgaga tcgcctttca ggcataaaaa ctgcttgggg
    34201 taaagtacgc gggaaagcgg ccataaacgg ctaccggagc cacctgccat cacaactgga
    34261 tagagtttcg actgcgccat aattatcccc gaatatcatt tataaattga cgtaacacgt
    34321 tctctttatc gagcgtgcgt tcggcatatt cacgtgccac cgtgttatgt ttgggcagca
    34381 ggagcgcctg acgaatcccc gccaccagcg cctcgaccga ttccggttct acgcaaaccg
    34441 caatacccgg aaaggtttcg caaagctgcc ccagttctgt gtgggcctct gcggtaatca
    34501 ccgcgttacc gcctaccgcc agaatattgg tcagtttcga cggcaatacg gcatctgccg
    34561 cgccgcgttt ttgcaccacc agatggcaat cgcccatctt cagcagtgca ggtaaagcgt
    34621 catacgattg cagcgggaaa aattgcatgt tgcgcagtcc acgctgctgc gccatttttt
    34681 ccagccgcgc tttgccgccg ctctgcccga caatggcaaa aatcagcggt tcatcgcgca
    34741 ggagatcggc agcttcaata acgttttcca gcccctgctt ttcaccaata ttgccggagt
    34801 aaagaatgat ttttttgtta tccggcaggc caagctggtt acgaagggca tcaacatcgg
    34861 cgtctgcaac atgctgaaaa cgggcaattt ccgaccagtt ggggaagaag atgacgttat
    34921 ccgccgccac gcctttttcg atggctttat tcatcatcga acgcgaaatc gtggaaacgt
    34981 tatcgacgtt atgcagtccg ctacgttcga acgccgttgc cagctgtgcc actttgccgc
    35041 ctttgccttt tccggcaagg cccagcccca gcatggcgtc cacttcgtaa tcctgaatat
    35101 gcagcacggt acgcgcacca gagagcttcg ccagcaggcg cattcccggc gtgcaaaaca
    35161 gcgttggcac tacgccaata atgcgatccg gcttccagcg acgttgcgcc atcagtggaa
    35221 agaaactgct gacggcaaaa ctgccgagat gcaacaagcg tttcagggtg ctcggctgtt
    35281 ttggcacgta cagcgggcag cgccacaccg tggcagcccc ttcttctcgt ttgtagcgcc
    35341 aggcggaata gttctcaccc acctgccatt gcgggtagta aggcggtgcg gtaatgaccc
    35401 gcacctcatg accttgtgcc gccaaccatt ccaccatctc gccggtgtat ttgccgatgc
    35461 cggttaactc cggcgagtag ttaatgccgt agaccagtat tttcataatc cgggtactcc
    35521 ggcacgcttc tcagcgagaa aataggctcg gctgttagcg tgaacatcgt tactggcgag
    35581 cagcgcgtcc ggcgtcagcc agcggtaatc gtcatgctgc tcatccggca gtaacagctc
    35641 ttcttccgct actctgaagc gaaaaccgag caccacatag tgagtggtga aatccgtgcc
    35701 ggagaagtta tcgtcataaa aatgctgcca gacaccgtaa aactggcctg ctgttatcgg
    35761 cagacgcagc cccagttccg ccatcgtcag ccgctcaaat gcggcttcca gcgtttcgtc
    35821 tttctgcacg cgccctcccg gcacaaacca gtaaccctgc gccgggcggt tggttctttt
    35881 gccaagcaga aactcgccgc gactgttctc gacaataaag tcgagagaga caagcggtgt
    35941 ggagcgcact acagtggcaa agtcttcctg acgtaaaaac atcattcccc ccgaaagcgg
    36001 tcttgattct caaggaacca ctggtaagtg ctggcaagcc ccgcttccag tgagatttcg
    36061 tgataccagc caagctgatg caggcgcgtc acatccagca gtttgcgcgg cgtaccatcc
    36121 ggtttgctgg catcaaaaac cacccgacct ttgtaaccca ccactttggc gatggtttgc
    36181 gccagctcgc ggatagtgca gtcaacgccc gtgccgacgt taatgtgcga cagcatcggt
    36241 tgggtattct ccagccagac ttcatgcgcc agctccatga catgaatgct cgccgccgcc
    36301 atatcatcga cgtgcaggaa ttcacgcatc ggtgtaccgc tgccccatac caccacgtcc
    36361 gtgccgtcgc ctcgtggaag cgacgcagca atgctggtat cacatgggag ttactcgggt
    36421 ggaagttgtc atgcggcccg tacaggttgg tcggcatgac agtgcggtaa tcgcgtccat
    36481 actggcggtt gtaagattcg cacagtttga tcccggcgat tttggcaatg gcgtaaggct
    36541 cgttggtcgg ctccagcgtg ccctgcaata actcgctttc tgccatcggc tgttttgcca
    36601 gtttcgggta gatgcaggac gatccgagaa acatcagttt gttcacgtcg ttctgatgcg
    36661 cggcgtgaat gatgttgctc tcaatcatca tgttctggta gatgaaatcc gccggatagg
    36721 tgttgttggc aacaatgccg cccactttcg ccgccgccag atagacctgg tcaatgcgtt
    36781 cgctggcaaa gaaatcatgc acggcgcggc tgtccagcag gttcagttca tcgcgggtac
    36841 gtaataccag ctccacatca ccgcgctgtt cgagctgccg cttgatggca gaaccgacca
    36901 tcccacaatg gccagcgata aaaatgcgtt gtttactcat gcttatgact ccagcgcgat
    36961 cgccacgtcg tagccgtgag atttcagcag agagtgtttt ttcgccgctt cgaggtcatt
    37021 agccaccatt tcagacacca tctctctgag ggtgatttcc ggtttccagc ccagtttttc
    37081 gtgcgctttg gtcgggtcgc cgagcagcgt ttcaacttct gccggacgga agtaacgcgg
    37141 atcaacggcg ataatcacat cacccggttt aacgcccggc gcgtcatgcc cggtgacgga
    37201 aaccacaatg cccttctctt caacacccgt gccttcaaag cgcagtttga tgcccagctg
    37261 tgctgccgcc atttccacaa actgacgtac ggagtactga acgccggtcg cgataacgaa
    37321 atcttccggc tgttcctgtt gcagcatcat ccactgcatt tttacgtagt ctttggcgtg
    37381 gccccagtca cgcagggaat ccatattgcc gaggtacagg cacgactcca gcccctgggc
    37441 gatgttggcg attgcgcggg tgattttgcg ggtaacgaag gtttcgccgc ggcgcgggga
    37501 ttcatggttg aagagaattc cgttacaagc gtacatgccg taggattcgc ggtagttaac
    37561 ggtgatccag taggcgtaca gtttggcgac cgcatacgga gatcgcgggt agaacggcgt
    37621 ggtttctttc tgcggaattt cctgcaccag accatacagt tcagaggtgg aagcctgata
    37681 gaaacgagtt ttcttttcca gaccgaggaa gcggatcgcc tccagcaggc gcagcgtacc
    37741 catcgcgtcg acgtcagcgg tatattctgg tgactcaaaa gagaccgcaa cgtggctcat
    37801 tgcgcctagg ttgtacactt catccggctg tacttcacgc aaaatgcgcg tcaggttaga
    37861 ggtatcactc aggtcgccat aatgcagatg gaatttcggg ttgcaggtgt gcggatcctg
    37921 ataaatgtga tccacgcgct cggtgttgaa tgacgatgcg cgacgcttaa taccatgcac
    37981 ctcgtaacct ttttccagca gaaactctgc caggtaagaa ccgtcttgtc cggttacacc
    38041 ggtgatgaga gcgacttttg acatgtctta ttcctctgta tttttgaatt tattcagttt
    38101 caacgcgttc gcgtatcacc actgcgggat tcccccggca aaccacattt gccggaagcg
    38161 atttaaaaac actgcttcgt gcacccacga cggtgccgtc gccgattgtg acgcctgggg
    38221 caacaaagac atcggttgcc agccagcatt tctcgccaat cacaataggc gtggcgttaa
    38281 tggtgaaatg ttgacttgca tggtcgtggc taccggtgca taaataactt ttttgcgata
    38341 tcaccgaatg tgcgccaatg gttatttcac cgagggtata taaattgacg tcatcgccaa
    38401 cccacgcgta atcacctaag gttaatttcc acggataggt aatttttact gacggacgaa
    38461 taactacgtt ttttcctatt tttgctccga ataaacgtaa taaaaaagcc cgccagcgat
    38521 acaatacttg tggcgaccag gcaaatattg ttgcctgtac tgcccaccat aattgcactt
    38581 taatagcgtt gccgccccgg aaccctttcg gcaccgagaa accgcttaaa tcttgcattg
    38641 gttttcctta tattcagact ttgttatata aggctttagt cttacctgtc gtacgttggc
    38701 gtaaatgcca ggataattct gcccagaagc caggtacatg taatatctgc cgttggactt
    38761 ttttggcgtc agcac
//
LOCUS       DADLCQ010000027        37629 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000027 DADLCQ010000000
VERSION     DADLCQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.027"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..175)
                     /locus_tag="JQG95_001222"
     CDS             complement(<1..175)
                     /locus_tag="JQG95_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY5887847.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JQG95_001223"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="JQG95_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY5887848.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JQG95_001224"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="JQG95_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY5887849.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JQG95_001225"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="JQG95_001225"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY5887850.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JQG95_001226"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="JQG95_001226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY5887851.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JQG95_001227"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="JQG95_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY5887852.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="JQG95_001228"
     CDS             complement(5179..5421)
                     /locus_tag="JQG95_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY5887853.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JQG95_001229"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="JQG95_001229"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY5887854.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGKRCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JQG95_001230"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="JQG95_001230"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY5887855.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="JQG95_001231"
     CDS             complement(8338..9312)
                     /locus_tag="JQG95_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5887856.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="JQG95_001232"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="JQG95_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY5887857.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /locus_tag="JQG95_001233"
     CDS             11709..12752
                     /locus_tag="JQG95_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY5887858.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="JQG95_001234"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="JQG95_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY5887859.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="JQG95_001235"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="JQG95_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY5887860.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="JQG95_001236"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="JQG95_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY5887861.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="JQG95_001237"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="JQG95_001237"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY5887862.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /locus_tag="JQG95_001238"
     CDS             16538..20338
                     /locus_tag="JQG95_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY5887863.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAAGKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="JQG95_001239"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="JQG95_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY5887864.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JQG95_001240"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="JQG95_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY5887865.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="JQG95_001241"
     CDS             23179..23382
                     /locus_tag="JQG95_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY5887866.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="JQG95_001242"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="JQG95_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY5887867.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="JQG95_001243"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="JQG95_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY5887868.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="JQG95_001244"
     CDS             26387..26659
                     /locus_tag="JQG95_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887869.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JQG95_001245"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JQG95_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY5887870.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JQG95_001246"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JQG95_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY5887871.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JQG95_001247"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JQG95_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY5887872.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JQG95_001248"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JQG95_001248"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY5887873.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JQG95_001249"
                     /pseudo
     CDS             complement(30405..31752)
                     /gene="degQ"
                     /locus_tag="JQG95_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31906..32304)
                     /locus_tag="JQG95_001250"
     CDS             complement(31906..32304)
                     /locus_tag="JQG95_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY5887874.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32498..33625
                     /locus_tag="JQG95_001251"
     CDS             32498..33625
                     /locus_tag="JQG95_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY5887875.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="JQG95_001252"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="JQG95_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY5887876.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="JQG95_001253"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="JQG95_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY5887877.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="JQG95_001254"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="JQG95_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY5887878.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="JQG95_001255"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="JQG95_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY5887879.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQG95_001256"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="JQG95_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY5887880.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9114 a         9416 c         9898 g         9201 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 actaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaaaggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccataccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagt gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggtcaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gccaggtgca taccgaacat caggattgcg actataatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcgtctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgtttgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgtgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaatataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtggttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat accgctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgacgttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgca
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcaca
    14761 ttctcgattg tctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgtc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttaacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag cgcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc ggacagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 aggcaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacttggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacgtt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaaact cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgccgc gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggacgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg atgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactctg accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt gatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgattcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccacgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtatacac taattattga
    27061 actaattaat accacactaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 taggcaaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggccga
    30661 cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt ggtttgccgt tacgcagcag
    30721 gccaagcttc actttcgtgc ccggctctgt agtcgcgata cgagagcgca actcagcaaa
    30781 gctattcagc ggtttgccgt tgaggctggt aataatatcg cccgctttga cgcccgcttt
    30841 cgctgagcca gaacttggca acacttcgct gacaaacgcg ccacgctgca cgtcaaggtt
    30901 gaaggctttg gcgatatcgg cactcatctc ggtgcctttg atgcctaaca aaccgcgttt
    30961 gatttcacca aagtcgataa gctgctgcgc cagtgttcgc gccatattac tggggatggc
    31021 aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt gcagtgttga tgccaattaa
    31081 ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg cggttaatgg aagcatctgt
    31141 ctggataaag ttttccagac cttcaagatt caacccgctg cggcctaatg cggaaacaat
    31201 gccagaggtg gcggtttgcc caaggccaaa tgggttaccg accgctacgg caaaatcacc
    31261 gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt aatttgctcg ggttttgaat
    31321 ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc agttttgcat caaactcgcg
    31381 cccatcattg agctgaatac tgattttctg tgcctgatta atcacatggt tgttggtcag
    31441 cacatagcct ttactggcgt tgatgatgac accggaacct aaaccttcga agggttgtgc
    31501 aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat tcttccggga ttttctgtcc
    31561 ctgactggcc gttccttcca cccgtacgct caccactgcc ggaagcactt tttccagcat
    31621 tggagccaga ctggggagag gggcctgatc ggcaacctgg cctggaatcg acgcgacggc
    31681 ctgaaatgac gccgagagag ttaacccggc acttaacgct aatgcactca acagctgggt
    31741 ttgttttttc attattcctg ctctcgtacc tgaatgataa gaaaagagat tcaaaacagt
    31801 ttgatgttat tgaattttca gacggctaac aatgagacac cagattaaat gatagtcggg
    31861 gagggagaga agaggagggc gcaatggctg cgcccgaaaa ataaattagt cgcgcttcgc
    31921 gccagtacgc agcaggccgg atgcgccttc agaatagtcg cgaggcacct gcaccggtgc
    31981 ctgatcgttg ctggcttcag actctgccag acgattacgg aacgggtttg cttcagcaga
    32041 cagttccggc agcaggctgc tggagctttt tgccatgtgc tgatacagct ggcgatagtc
    32101 gtgcgccatg gtatccagta attccgcgct gcgggcaaag tggctaacca gctcttcgcg
    32161 atactcgtcc agttcagctt tattcttttc cagttcgtac tgcaacgcct gttgctggcg
    32221 tagtttacga ttaccaaaac gcatggccac agcaccaata atgatgccga cgactaaccc
    32281 aattagcgca tattcccagg tcatgaacat ctcccgttgt cttttgtttc cgtagggtgt
    32341 tggcttcagg ctccatgcct gcggctgatt atgccactat aaccgttaat tccacagaag
    32401 tggaatcccg actgcatatc gcgtagtgta gaacggcctt tttttcgtca accgtgaaca
    32461 acagcgcacc gattattcaa ggaataacaa taagatcatg caaagcgtta ccccaacatc
    32521 gcaatacctg aaggcgctca atgaaggcag ccatcaaccc gacgacgttc aaaaagaggc
    32581 cgtcagccgc ctggaaatta tttatcaaga actcatcaat agcaggccac cagcccccag
    32641 gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt aaacgcgaag acacaaagca
    32701 tacgccagtg cgtggcttat atatgtgggg cggtgtagga cgcgggaaaa cctggctgat
    32761 ggaccttttc tatcaaagcc tgccgggaga gcggaaacag cgcctgcact ttcaccgttt
    32821 tatgctgcgg gtgcacgaag agctaactgc cttacagggg cagaccgatc cgctggaaat
    32881 tattgccgat cgctttaaag ccgaaactga cgtgctctgt tttgacgaat tttttgtttc
    32941 tgatattacc gacgccatgc tacttggcgg tctgatgaaa gccctgttca cccgcggtat
    33001 taccctggta gcgacgtcaa atattccgcc ggacgaactt tatcgaaatg gcctgcaacg
    33061 tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat tgtgatgtaa tgaacgtgga
    33121 cgctggtgtt gattatcgac tgcgtacact cactcaggcg catctgtggc tttcgccact
    33181 caacgatgaa acccgggcgc agatggataa actatggttg gcgctggcgg gggtgaaacg
    33241 agaaaattca ccgacgttag aaatcaacca tcggccattg gcgacaatgg gcgtcgagaa
    33301 ccagacgctg gcggtttctt ttactacgct gtgcgtcgac gcccgcagtc agcatgacta
    33361 tattgcgctc tcacgcctgt ttcacacagt aatgttgttt gatgtgccag tcatgacgcg
    33421 gttgatggag agcgaagcgc ggcgctttat tgcgctggtg gatgagtttt acgagcgcca
    33481 tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa atttatcagg gtgagcggct
    33541 gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag atgcaaagcg aagagtatct
    33601 gaagcgcgag catttggcgg gttaaaacct gccacaaatc acaaaaaggg gtcgatcttt
    33661 gaccccgact tctctataat cctgcgaccc cacgttacaa gaaagttttt ttccccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgt tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacattg
//
LOCUS       DADLCQ010000028        36833 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000028 DADLCQ010000000
VERSION     DADLCQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.028"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..247
                     /locus_tag="JQG95_001257"
     CDS             <1..247
                     /locus_tag="JQG95_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887881.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JQG95_001258"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JQG95_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY5887882.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JQG95_001259"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JQG95_001259"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY5887883.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JQG95_001260"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JQG95_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY5887884.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JQG95_001261"
     CDS             complement(3258..3542)
                     /locus_tag="JQG95_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887885.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JQG95_001262"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JQG95_001262"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY5887886.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JQG95_001263"
     CDS             complement(4443..5651)
                     /locus_tag="JQG95_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887887.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /locus_tag="JQG95_001264"
     CDS             5735..6271
                     /locus_tag="JQG95_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5887888.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JQG95_001265"
     CDS             complement(6446..8131)
                     /locus_tag="JQG95_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY5887889.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLDFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JQG95_001266"
     CDS             8401..8778
                     /locus_tag="JQG95_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887890.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JQG95_001267"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JQG95_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY5887891.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JQG95_001268"
     CDS             9225..9512
                     /locus_tag="JQG95_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY5887892.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JQG95_001269"
                     /pseudo
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JQG95_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JQG95_001270"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JQG95_001270"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY5887893.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JQG95_001271"
     CDS             11269..11745
                     /locus_tag="JQG95_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY5887894.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JQG95_001272"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JQG95_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY5887895.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="JQG95_001273"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="JQG95_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY5887896.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JQG95_001274"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JQG95_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY5887897.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDSLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JQG95_001275"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JQG95_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY5887898.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JQG95_001276"
     CDS             16302..16790
                     /locus_tag="JQG95_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY5887899.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JQG95_001277"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JQG95_001277"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY5887900.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JQG95_001278"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JQG95_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5887901.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JQG95_001279"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JQG95_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY5887902.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JQG95_001280"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JQG95_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY5887903.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JQG95_001281"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JQG95_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5887904.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JQG95_001282"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JQG95_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY5887905.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JQG95_001283"
     CDS             complement(22242..22757)
                     /locus_tag="JQG95_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5887906.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="JQG95_001284"
     CDS             23406..25175
                     /locus_tag="JQG95_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY5887907.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="JQG95_001285"
     CDS             25386..25709
                     /locus_tag="JQG95_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY5887908.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /locus_tag="JQG95_001286"
     CDS             25706..26536
                     /locus_tag="JQG95_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY5887909.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="JQG95_001287"
     CDS             complement(26533..27546)
                     /locus_tag="JQG95_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY5887910.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="JQG95_001288"
     CDS             complement(27645..29075)
                     /locus_tag="JQG95_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5887911.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JQG95_001289"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JQG95_001289"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY5887912.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JQG95_001290"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JQG95_001290"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY5887913.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JQG95_001291"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JQG95_001291"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY5887914.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JQG95_001292"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JQG95_001292"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY5887915.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JQG95_001293"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JQG95_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY5887916.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JQG95_001294"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JQG95_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY5887917.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT         9214 a         9156 c         9440 g         9023 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc acgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagtca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggataccta gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgctacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgctgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg attcgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaattggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata actcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata attgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaattac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaa
//
LOCUS       DADLCQ010000029        35625 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000029 DADLCQ010000000
VERSION     DADLCQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.029"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQG95_001295"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JQG95_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY5887918.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JQG95_001296"
     tRNA            complement(621..695)
                     /locus_tag="JQG95_001296"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="JQG95_001297"
     CDS             complement(769..1701)
                     /locus_tag="JQG95_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY5887919.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHAELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="JQG95_001298"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="JQG95_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY5887920.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3991)
                     /locus_tag="JQG95_001299"
                     /pseudo
     CDS             complement(2932..3991)
                     /locus_tag="JQG95_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="JQG95_001300"
     CDS             complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="JQG95_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY5887921.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6069..6353
                     /locus_tag="JQG95_001301"
     CDS             6069..6353
                     /locus_tag="JQG95_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY5887922.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6533..7843
                     /gene="fadI"
                     /locus_tag="JQG95_001302"
     CDS             6533..7843
                     /gene="fadI"
                     /locus_tag="JQG95_001302"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY5887923.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7843..9987
                     /gene="fadJ"
                     /locus_tag="JQG95_001303"
                     /pseudo
     CDS             7843..9987
                     /gene="fadJ"
                     /locus_tag="JQG95_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
     gene            10190..10675
                     /gene="sixA"
                     /locus_tag="JQG95_001304"
     CDS             10190..10675
                     /gene="sixA"
                     /locus_tag="JQG95_001304"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY5887924.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            11359..11922
                     /locus_tag="JQG95_001305"
     CDS             11359..11922
                     /locus_tag="JQG95_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5887925.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
     gene            12004..14645
                     /gene="yfcU"
                     /locus_tag="JQG95_001306"
                     /pseudo
     CDS             12004..14645
                     /gene="yfcU"
                     /locus_tag="JQG95_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            14665..15387
                     /locus_tag="JQG95_001307"
                     /pseudo
     CDS             14665..15387
                     /locus_tag="JQG95_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            15392..15940
                     /locus_tag="JQG95_001308"
     CDS             15392..15940
                     /locus_tag="JQG95_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5887926.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            15937..16407
                     /locus_tag="JQG95_001309"
     CDS             15937..16407
                     /locus_tag="JQG95_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5887927.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            16404..16928
                     /locus_tag="JQG95_001310"
     CDS             16404..16928
                     /locus_tag="JQG95_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5887928.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            16915..17805
                     /locus_tag="JQG95_001311"
     CDS             16915..17805
                     /locus_tag="JQG95_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY5887929.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(17909..18460)
                     /gene="smrB"
                     /locus_tag="JQG95_001312"
     CDS             complement(17909..18460)
                     /gene="smrB"
                     /locus_tag="JQG95_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY5887930.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            18626..19558
                     /gene="prmB"
                     /locus_tag="JQG95_001313"
     CDS             18626..19558
                     /gene="prmB"
                     /locus_tag="JQG95_001313"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY5887931.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            19593..20678
                     /gene="aroC"
                     /locus_tag="JQG95_001314"
     CDS             19593..20678
                     /gene="aroC"
                     /locus_tag="JQG95_001314"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY5887932.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            20682..21506
                     /gene="mepA"
                     /locus_tag="JQG95_001315"
     CDS             20682..21506
                     /gene="mepA"
                     /locus_tag="JQG95_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY5887933.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            21506..22315
                     /locus_tag="JQG95_001316"
     CDS             21506..22315
                     /locus_tag="JQG95_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY5887934.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            22315..22863
                     /locus_tag="JQG95_001317"
     CDS             22315..22863
                     /locus_tag="JQG95_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY5887935.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            22897..23175
                     /locus_tag="JQG95_001318"
     CDS             22897..23175
                     /locus_tag="JQG95_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY5887936.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(23296..25302)
                     /gene="mnmC"
                     /locus_tag="JQG95_001319"
     CDS             complement(23296..25302)
                     /gene="mnmC"
                     /locus_tag="JQG95_001319"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY5887937.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            25461..26702
                     /gene="fabB"
                     /locus_tag="JQG95_001320"
     CDS             25461..26702
                     /gene="fabB"
                     /locus_tag="JQG95_001320"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY5887938.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            26957..28135
                     /locus_tag="JQG95_001321"
     CDS             26957..28135
                     /locus_tag="JQG95_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887939.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(28132..29127)
                     /locus_tag="JQG95_001322"
     CDS             complement(28132..29127)
                     /locus_tag="JQG95_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY5887940.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            29226..30362
                     /gene="pdxB"
                     /locus_tag="JQG95_001323"
     CDS             29226..30362
                     /gene="pdxB"
                     /locus_tag="JQG95_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY5887941.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30428..31441
                     /locus_tag="JQG95_001324"
     CDS             30428..31441
                     /locus_tag="JQG95_001324"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY5887942.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            31441..32253
                     /gene="truA"
                     /locus_tag="JQG95_001325"
     CDS             31441..32253
                     /gene="truA"
                     /locus_tag="JQG95_001325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY5887943.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            32336..32995
                     /locus_tag="JQG95_001326"
     CDS             32336..32995
                     /locus_tag="JQG95_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5887944.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            33151..34065
                     /gene="accD"
                     /locus_tag="JQG95_001327"
     CDS             33151..34065
                     /gene="accD"
                     /locus_tag="JQG95_001327"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY5887945.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            34135..35403
                     /gene="folC"
                     /locus_tag="JQG95_001328"
     CDS             34135..35403
                     /gene="folC"
                     /locus_tag="JQG95_001328"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY5887946.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            35393..>35625
                     /gene="dedD"
                     /locus_tag="JQG95_001329"
     CDS             35393..>35625
                     /gene="dedD"
                     /locus_tag="JQG95_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY5887947.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAG"
BASE COUNT         8518 a         8640 c         9403 g         9064 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagaaat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaatggttt ttgaactctt
     3901 gtactaatcc attaatttca tcattgttat tttcattgat ggattctggg taagctgcgc
     3961 tggtattgtt gattgaaata gatggtagca tgattttatt ccatgttgat tgatttgtta
     4021 caaatatata ttacctaatg aaatagacta tcaaaaaacg ctcatttatt tagaacctat
     4081 ctttaatttg agtgtataag cgcacaatag ccaggggggt tgagtatcct ggaaatgcag
     4141 aaaagtaaac tctttgagaa ggacgagcag aacgcttgaa aatatgcatt tcccttcagg
     4201 tttgtctgtg gatactactt actgatttga aagggcaagc tgccatcagg ctgtttgcaa
     4261 aactcaggag gcaagcaatg aggaaactac tttcggtgaa gtgggggagt taaagtcacc
     4321 tgcatagcag gtgactttat ctaggcgaac gcgttatcag aacgcgtagt taaagttagt
     4381 accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt taattttcac
     4441 gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta
     4501 agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg
     4561 agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta
     4621 gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa
     4681 cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta
     4741 agccaggcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca
     4801 catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg
     4861 aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa
     4921 gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata
     4981 caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt
     5041 attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc
     5101 aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat
     5161 tttcgcgcga gcgtagatgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg
     5221 atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc
     5281 gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc
     5341 ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg
     5401 cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc
     5461 cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc
     5521 ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg
     5581 gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga
     5641 gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac
     5701 cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa
     5761 gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca
     5821 gctagaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc
     5881 agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat
     5941 ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact
     6001 ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga
     6061 aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg
     6121 gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaaaccgcg
     6181 cagaagcaga acaaacgctg gcggcgctga ctgaaaaatc ccgtagcgtg gaatccgaac
     6241 cgtgcaaaat ctccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt
     6301 tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct
     6361 cgggtacgta cgcccctgtc agttgctggc aggggcgttt tcttttcttt cttctgtctt
     6421 cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt
     6481 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
     6541 ggttttacca ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
     6601 gccttttgcc cgccaggcga cggcttttca tggcattccc acggttgatt tagggaagat
     6661 ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt
     6721 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
     6781 taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
     6841 ccaggcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc
     6901 cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgcacgt
     6961 gctggttgat gtcaacaaag ctcgaaccat gagccagcgc ctgaaactct tctctcgcct
     7021 gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg
     7081 gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga
     7141 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
     7201 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
     7261 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
     7321 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
     7381 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
     7441 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac
     7501 accgctggcg ctggaacgtg ccggtttgac gatgagcgaa ctgacattga tcgatatgca
     7561 cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc
     7621 tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
     7681 gcttggcggt tcgattgctt acggacatcc cttcgcggcg accggcgcgc ggatgattac
     7741 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
     7801 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat ggcatcagcg
     7861 tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt accggatgag
     7921 aaaatgaata ccctgaaggc ggaatttgcc tcgcaggtgc gcgccattat taagcaactc
     7981 cgtgaaaaca aagagttgcg cggcgtggtg tttatctccg cgaaaccgga caattttatc
     8041 gccggagcag acatcaacat gattggcaac tgcaaaacgg cgcaagaagc ggaagcactg
     8101 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg
     8161 gctattcatg gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc
     8221 gtttgtactg acgatcctaa aacggtgctc ggtttgtctg aagtacaact tggattgtta
     8281 cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagtac agcattagag
     8341 atgattctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat
     8401 gacgttgttc cgcactccat tttgctggaa gccgctgttg agctggcgaa gaaggatcgc
     8461 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
     8521 ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg
     8581 acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggctat
     8641 gacgctgaag ctcgcgcctt tggcgaactg gcgatgacgc cgcaatcgca agctttgcgt
     8701 agtatctttt ttgccagtac ggacgtgaag aaagatcccg gtagtgatgc accgcctgcg
     8761 gcattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
     8821 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
     8881 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
     8941 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
     9001 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
     9061 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct
     9121 ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat
     9181 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
     9241 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc
     9301 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
     9361 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt
     9421 ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
     9481 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
     9541 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
     9601 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc gctcattggc
     9661 gcacaagggc aggggtgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
     9721 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
     9781 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
     9841 gattctctcg gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt
     9901 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
     9961 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    10021 gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    10081 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    10141 tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    10201 tatcatgcgt cacggcgacg cagccctcga ttccgccagt gattcggttc gtcctctgac
    10261 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga
    10321 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    10381 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    10441 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    10501 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    10561 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    10621 tacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgtgt
    10681 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcattag gcagcggata
    10741 tctgtcaacc tcactactat cgaagactct ccttatctat aaataaaaca ctttctacat
    10801 tcattcggaa attattcttc tgcgcgtatc ttgatttttc ccaaagttac atgttgaaat
    10861 gaataatatc tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag
    10921 ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata gtgtttatat
    10981 attatattgt caaaagtgga taaatgccaa tcgcattata tgttcattgt ttgtttttta
    11041 agctgttgat attcattatt gatatacttt ctattttttc taaatactta ttccgtattt
    11101 tttaaggatg tccttaagat aacctttctt ctgtcaatat attaattaaa tctacattta
    11161 ctctttagta aaattaaatt ggatcagggg tgtggaaaca tcactatgcg gccccacacc
    11221 aatgaataca attttctacg acctgttctt tttgggtcgc atgccgtatt caatggctgg
    11281 gtataaacat tggctattga ctgaatcaat gtattgcaat gtttatattt ttaattattt
    11341 atagtttaag gacaaataat gagtaagttt gttaaaacag ctattgctgc ggcaatggtg
    11401 atgggcgtgt tcacctctac ggcaacaatc gctgcgggta acaacggtac tgcacgtttt
    11461 tacggcacca ttgaagactc agtgtgttct atcgttccag acgatcacaa actggaagtt
    11521 gatatgggtg atatcggtgc cgaaaaactg aaaaataacg gcaccaccac gccgaaaagt
    11581 ttccagattc gtctgcaaga ttgcgtattt gatactcagg aaacaatgac cactaccttt
    11641 actggtaccg tttcttctgc aaatagcggc aattattaca ccattttcaa taccgatacc
    11701 ggtgcggcat ttaacaatgt tagcctggcg atcggtgact ctctgggtac ctcttacaaa
    11761 agcggcatgg gtattgacca gaaaatagtg aaagacactt ctaccaacaa aggcaaagcg
    11821 aagcagacac tgaactttaa cgcctggctg gtcggcgcag ctgatgcgcc agatctgggt
    11881 aattttgaag caaataccac cttccagatt acttacctgt aaggcatacc cccatagcga
    11941 cactgctatg ggggatttaa atgtattttg accgaacaag aataaaaacg gatagaaacg
    12001 tgtatgcctg accattctct ttttcgatta cggattcttc cgtggtgcat tgcgctggca
    12061 atgtcaggga gttatagcag tgtctgggct gaagacgaca ttcagtttga ttcccgtttt
    12121 ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt tttccagcta gggatatgtt
    12181 gagcccggaa aatacaattt acaggttcaa ctaaataaac agccattggc ggaagagtac
    12241 gatatttact ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg tctgacaccg
    12301 gaactggtag cgcagtttgg tttaaaagaa gacgtggcga aaaatctgca atggagccac
    12361 gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc tgatttaagc
    12421 cagtccgcat tagtcatttc actgccacag gcttacctcg aatatactta tcccgactgg
    12481 gatccgcctt cacgttggga tgacggcatc tccgggatcg tcgcggacta cagcatcaac
    12541 gcacaaaccc ggcacgaaga aaatggcggt gatgatagta acgagatcag cggcaacggg
    12601 acggttgggg ttaacctagg gccgtggcgt atgcgtgctg actggcagac taactatcaa
    12661 catactcgca gtaatgatga cgatgaattc agcggcgatg aaactcaaaa aaaatgggag
    12721 tggagtcgct actatgccag gcgggcgtta ccatcattaa aagccaaact ggcgctgggc
    12781 gaggattacc tcagatccga tatttttgat ggttttaact atgttggtgg cagtgtcagt
    12841 actgacgatc aaatgttgcc tcccaatctg cgcggctacg cgccagacat ttccggcgtg
    12901 gcacacacca cagcaaaagt gaccgtcagc cagatggggc gtgtgattta cgaaacgcag
    12961 gttccggctg gaccgtttcg tattcaggat cttggtgatt ctgtctccgg tacgttgcat
    13021 attcgcattg aagaacagaa cggccaggtg caggaatatg acatcagcac cgcctcgatg
    13081 ccatacctca ctcgtccagg tcaggttcgc tataagatca tgatgggccg tccgcaagag
    13141 tggggacacc atgtcgaggg tgaatttttt tctggtgctg aagcttcctg ggggatcgct
    13201 aacggctggt cgttatatgg cggcgcactg ggagatgaaa actatcagtc tgcggcgctt
    13261 ggtgtcggtc gcgatttgtc tacattcggc gcggtcgcgt ttgatgttac tcactcgcac
    13321 accaaactgg ataaagacac cgcttatggc aaaggttcgc tggacggtaa ctccttccgt
    13381 gtgagttatt ccaaagactt tgaccagctc aacagccgcg ttacttttgc tggatatcgc
    13441 ttctcggaag agaactttat gaccatgagc gagtatctgg atgccagtga cagcggaatg
    13501 gtacgcacgg gcaacgacaa agagatgtac accgccactt ataaccagaa cttccgcgat
    13561 gcgggtgttt cggtttatct caactatacc cgccatacct actgggatcg cgaggagcag
    13621 acaaactaca acatcatgct ctcgcactat ttcaatatgg gcagtattcg taatgtcagc
    13681 atctcgatga ctggctaccg ttacgagtat gacaaccagg ccgacaaagg catgtacatt
    13741 tcgctcagta tgccgtgggg cgacaacagt accgttagct ataacggtac tatggcagtg
    13801 ggacggacag cagtcaggtc ggttatttca gccgtgtcga tgacgcgact cactatcagt
    13861 tgaacgtcgg caccagtgac aaacacacca gcgttgacgg ctattacagc catgatggtt
    13921 cgctggcgca ggttgacctc agtgcgaact accatgaagg gcaatacacc tctgcgggct
    13981 tgtcgttaca gggcggcgca acgcttactg cccacggtgg cgcacttcac cgtacccaga
    14041 atatgggcgg gacacgcttg ttgattgatg ccgatggcgt tgccgatgtt ccggtagaag
    14101 gtaacggtgc tgctgtttat accaatatgt ttggtaaagc cgtcgtttct gacgtcaata
    14161 actattaccg caatcaggcg tatatcgacc tcaacaaact gccggaaaac gccgaagcaa
    14221 cccagtcggt ggtacaagcc acgctaactg aaggggcgat tggctaccgc aaatttgccg
    14281 tcatcagtgg tcaaaaagcg atggcggtgc tgcgtttaca agacggcagc catcctccgt
    14341 ttggcgcaga agtaaaaaat gataacgagc agacagtggg ccttgtcgat gatgacggca
    14401 atgtttatct ggctggggtg aaacctggcg aacacatgag tgtgttctgg agtggtgttg
    14461 cgcattgcga tatcaacctg ccggacccgc tgcctgccga tctgtttaac ggcttgttac
    14521 tgccatgcca gcataaaggc aacgtcgcgc cggttgttcc tgatgacata aagcctgtca
    14581 ttcaggagca gacgcaacag gtgacaccca ccaatccccc tgtttctgtt tcagctaacc
    14641 aataacgtga ttaaggaatg atccatgtct gatttacttt gctctgccaa attgggtgct
    14701 acgacgctag cgttactgct tagcgctgcc agtttgtctg cccaggcttc tgtaacgcca
    14761 gatcgtaccc gtttgatatt taatgaaagc gacaaatcaa tcagcgtgac gttgcgcaat
    14821 aacgatccga aactgcctta tctggcccaa agctggatcg aagatgaaaa gggtaacaag
    14881 atcagttcac cgttaactgt tctgccaccg gtgcaacgca tcgattcaat gatgaacggg
    14941 caggtaaaag tgcagggaat gccggatatc aacaaattgc ctgccgatcg tgaaagcctg
    15001 ttctacttca acgtgcgcga gatcccgcca aaatcaaaca aagccaacac gctgcaaata
    15061 gcactacaaa cgcgtattaa gttgttctgg cgaccgaagg cgctggagaa cgtcagcatg
    15121 aaaaacccct agcaatataa gattacttta acccgcaacg gtcaggaatt taccgtcaat
    15181 aacccgacgt cgtattacgt cattttcagt aatgccagca cacagaaaaa tggcaatccg
    15241 gcagccggtt tctcgctaat agtgatgtcg gcgaaaacct ctgcaccgct gaatgtgaag
    15301 atgggtagcg tgccggtact gacctatgtc aacgactatg gcgctcggat gccgctcttt
    15361 ttcacttgca acggtaacag ttgctaggtc gatgaagagc agagccgcaa aggctgaggc
    15421 cgggagagcc agtatgaaag gatcttgttc tgtctgggcg gtaactgggt tactgatatg
    15481 ttgtgttggt agcggctttg cagttgcagc gagtaagtca gtaagtatga atttacgggt
    15541 gcttgtcgat gcccctcctc cttgtacggt aaatggtgcg gcagttgagt ttggtaatgt
    15601 attcattaat aagatcaata gggttgatta taaacgtccc attgactact cgcttgtttg
    15661 taacaatctg gcaatggatg acctgcgtct acagatgcag gcgacaacgg tcgtgattaa
    15721 cggtgaaaca gttatcagca cgggtattcc tgggtttggt attcgggttc agaaaagtag
    15781 tgatcacaca atactcgact taacctctgg ctcatggttg ccatttaatt tttcctcggg
    15841 agttcccgtt ctggaagctg tgccggtgaa acagagtggt acaacgttag cggcagctga
    15901 atttaacgcc agtgccacaa ttgtggtgga ttaccaatga aacggatctc gcttattttg
    15961 ctgtggggct tttgctcgat ggcactctca aacgtctctt ttcacggtta tttagtgcag
    16021 ccgcctaact gcacgattag caacgctcaa accattgaaa taacttttca ggatgtgctt
    16081 attgatgaca ttaatggcag caattatgag caaaccgtgc cgtattccat tacctgcgat
    16141 acggcggttc gcgacccatt aatggaaatg accttgagct ggagtggcac accaagcgat
    16201 tttgataatg ctgcagtaag cagcaatatc actggtttag gcattcagct caaacaggca
    16261 gggcagagtt ttacgatcaa tacgccactt gtggtgaatg aaaccgattt accggtgtta
    16321 acggctgtgc cagtaaaaaa gagtggtgtc attctgccag aggcggactt tgaggcctgg
    16381 gccacgttac aggtagatta ccaatgaaga aaaagcgtac gttgtttttt attagtagcc
    16441 tgatgttgct gggcagtgga accacaatag caggagataa tttgcacttt accggcaatt
    16501 taatcagtaa atcgtgtacg ccagtgatta atggtagcca gttggcagaa gtacattttc
    16561 ctgccattgc cgccagtgat ttaatgaacc ttgggcagtc agagcgagtg ccattggtgt
    16621 ttcaattgaa agattgtcat agttctacat tgtttaatgt caaagtaacg ttgacaggaa
    16681 cagaagatag tgctctaccc ggctttctgg cctttgactc ctcgtcatca gcatccggcg
    16741 ctgggattgg tattgaaact gcggcaggca catcggtgcc aatcaacaat acaacaggcg
    16801 tcacgctccc gctgaatcag ggaaataaca gcctaaattt taatacctgg ttacaggcaa
    16861 agagcgggcg agatgtaacg tccggagact ttagtgccac agtgacggca acctttgagt
    16921 atttctgaaa tgaaaaaatg gaccataatt ttcgcgagcc taatgctgct cgttctgagt
    16981 gttgtcggcg catctaagag ttatgctgga gataaactta tgagtgcgag ttttgacagt
    17041 gtgagagttt attatgccat ggacaaggta accggtgctg tagcgagcag tgttttcaat
    17101 gttacggtga taacgccaaa ggaggttgca tatgggaaat atgattcttt tgcgtataaa
    17161 ggcgatacgt tgcgggttat ttcctggtct gggtcgggtt cagctccaac tttggtactg
    17221 actgactttg atacaattaa taactcaaat tgccctggca tagatacgaa aatttttcgt
    17281 tgtgcatata tgacgtttaa aattaccgtt gcatctgatg attatggttg cccttggatc
    17341 gcatcctttt attcctatac agatttaccc ggatttggaa gctatacggc ccctacagtg
    17401 cataacacaa tatgcccaac gataccggtc gcttcatacg atatctcctg gagtgaaaat
    17461 tatgtcagcc ataataaggc gttacgaatt cagtccacgg ggtcaacagt gaccaccacg
    17521 ctttcgacct acctgatgga aggcggcaga ctctgtgatg gcagtaattt ttctgataat
    17581 gatggcagag gagcatactg ccgcgcggta tcagaattgt taacctttac ctcttacggt
    17641 tgtgataaat caacggtaac ggttacgcca actcgtcatc cggtaacgga taaagtacta
    17701 cacgatattg tggtgaatgt gaataccagt agtgggcaac cgattgattc gacgtgtcgt
    17761 tttcagtatg tattgaatga actgaagttc gtagtattgc tctgatgcgt cttatcaggc
    17821 ctacgaagtg aattgcaatt tattgaatta tcacatattt gtaggccgga taaggcgttt
    17881 acgctgctcc ggcatttgct gccagcattc agggcaactc cggcggcaac cattcttcca
    17941 cttcaatcaa caccaacaaa gccgcatcac cgccgtactc tttcggtgcc tggtgaaatg
    18001 ccatcacatg cggatgttgt gccagccaca gcggtgtttg ttgcttcaaa atatgcttcc
    18061 cgtgcccatg catcacgcag gcacaaaaca catgttcgcg gcgacaggcg gcaatcaacg
    18121 cgcccagttc ctgcttggcc tgcagctgcg tcagaccgtg taaatccaaa aacaattccg
    18181 gtgaataatc gccgcggcgc agtttcttcg cctcaaaatg gctgacatcc gggcgaacat
    18241 atttcaccgg accttcggta tttaataacg gctgaaactc atcggagaaa taatggctgg
    18301 catcagcctg ctcctggatc aagcgtttca ccggcacttc gctgattttt ttacgctgcg
    18361 gtcggtggac aatcgtgtcc tgcttaatct tgcgagtccc cgccatcaac tggcgaaaca
    18421 gagcctggtc ctcctcgcta agtgttgttt tctttttcat ttatctgtct ctatttctaa
    18481 ttttcctaca gtttacccga ctcgtagcac aatcgatcgt tttaaacgta tttttcctca
    18541 ctcaccgcgc gtgaatgctg atttatcgtc atcttcatgg caaactagcc gccgaaatta
    18601 ttgcgatcat gccctggagg aatacgtgga taaaattttc gttgatgaag cagtaaatga
    18661 gctgcaaacc attcaggaca tgttgcgctg gtcggtaagc cgcttcagcg cggcaaatat
    18721 ctggtacggt cacggcaccg ataacccgtg ggatgaagcc gtacagctgg tgttaccatc
    18781 gctctacctg ccgctggata tcccggaaga tatgcgcacc gcgcgtctga cctccagcga
    18841 aaaacaccgt attgttgaac gcgtgatccg ccgcgtcaac gagcgcattc cggtggctta
    18901 cctgaccaac aaagcctggt tctgcggtca tgaattttac gtcgatgaac gcgtgctggt
    18961 gccgcgctcg ccgattggtg aactgatcaa caataaattt gccggactca tcagcaagca
    19021 accgcagcat attttagata tgtgtaccgg tagcggctgc atcgccattg cctgtgctta
    19081 tgccttcccg gaagcggaag tcgacgcggt agatatctct cccgacgcgc tggcggttgc
    19141 cgaacagaac atcgaagaac acggtctgat ccacaacgtc attccgattc gttccgatct
    19201 gttccgtgat ttgccgaaag tgcaatacga tctgattgtc actaacccgc cgtatgtcga
    19261 cgcggaagat atgtccgacc tgccaaacga ataccgtcac gagccggaac tgggcctggc
    19321 atctggcacc gacggcctga aactgacgcg tcgcattctc ggtaacgcgg cagattacct
    19381 tgctgatgat ggcgtgttga tttgtgaagt gggcaacagc atggtacatc ttatggaaca
    19441 atatcccgat gttccgttca cctggctgga gtttgataac ggcggcgatg gtgtgtttat
    19501 gctcactaaa gagcagctta ttgccgcacg agaacatttc gcgatttata aagattaagt
    19561 aaacacgcaa acacaacaat aacggagccg tgatggctgg aaacacaatt ggacaactct
    19621 ttcgcgtaac caccttcggc gaatcgcacg ggctggcgct cggctgcatc gtcgatggtg
    19681 ttccgccagg cattccgctg acggaagcgg acctgcaaca tgaccttgat cgtcgtcgcc
    19741 ctgggacatc gcgctatacc acccagcgcc gcgagccgga tcaggtcaaa atactctccg
    19801 gtgtttttga aggcgttact actggcacca gcattggctt gttgatcgaa aataccgacc
    19861 agcgttctca ggattacagc gcaattaaag acgtcttccg tccaggccat gctgattaca
    19921 cctacgaaca aaaatacggt ctgcgcgatt atcgcggcgg tggacgttct tccgcccgcg
    19981 aaaccgccat gcgcgtggcg gcaggggcga ttgccaaaaa atatctcgcc gagaaatttg
    20041 gtattgaaat ccgcggctgc ctgacccaga tgggcgacat tccgctggaa atcaaagact
    20101 ggtcgctggt tgaacaaaat ccgttcttct gcccggaccc cgacaaaatc gacgcgttag
    20161 atgaactgat gcgcgcgctg aaaaaagagg gcgactccat cggcgcgaaa gtcaccgttg
    20221 ttgccagtgg tgtccccgcc ggacttggcg agccggtctt tgaccgcctg gatgccgaca
    20281 tcgcccatgc gctgatgagc atcaacgcgg tgaaaggcgt ggaaattggc gacggttttg
    20341 acgtagtggc gctgcgcggc agccagaacc gcgacgaaat caccaaagac ggtttccaga
    20401 gcaaccatgc gggcggcatt ttcggcggta tcagcagcgg gcagcaaatc attgctcata
    20461 tggcgctgaa accgacctcc agcattaccg tgccggggcg cactattaac cgctttggcg
    20521 aagaagttga gatgatcacc aaaggccgtc acgatccctg tgtcgggatc cgtgcagtgc
    20581 cgatcgctga agcgatgctg gcgatcgttt taatggatca cctgttacgg cagcgggcgc
    20641 aaaatgccga tgtgaagact gatattccac gctggtaaaa aatgaacaaa accgcgattg
    20701 cgctgctggc tctgcttgcc agtagcgcta gcctggcagc gacgccgtgg caaaaaataa
    20761 cccaacctgt gccgggtagc gcacaatcga taggcagttt ttctaatggc tgtattgtcg
    20821 gcgctgacac gttgccgata cagtccgaac attatcaggt catgcgtacc gatcagcgtc
    20881 gctatttcgg tcacccggat ctggtgatgt ttatccagcg tctgagtagc caggtgagca
    20941 atctgggcat gggtacggtg ctgattggcg atatggggat gcccgctggt gggcgtttca
    21001 acggcggtca tgccagccac cagaccggac tggatgtcga tatctttctg caactgccga
    21061 aaactcgctg gacctccgcg cagctcttgc gcccgcaagc actggactta gtttcccgcg
    21121 acggtaaaca cgttgtctcc acgctgtgga agccagaaat tttcagcttg atcaaactcg
    21181 ccgcccagga caaagacgtc acgcgcattt ttgttaatcc ggcgattaaa caacaacttt
    21241 gccttgatgc gggcaccgat cgcgactggt tgcgcaaagt gcgaccctgg ttccagcatc
    21301 gcgcgcatat gcatgtacga ttacgttgcc ctgccgatag tctggagtgt gaagatcaac
    21361 ctttaccgcc atcaggcgat ggttgcgggg cagaactgca aagctggttt gaacctccaa
    21421 aaccgggaac aacaaagcct gagaagaaga caccgcctcc gttgccgcct tcctgccagg
    21481 cgctactgga tgagcacgtg atctaatgga aacgtttaat agcctgttta tggtttctcc
    21541 gctgttgctg ggagttctct tttttgtcgc catgctggcg ggatttatcg actcgattgc
    21601 cggtggcggt gggttactca ccattccggc attgatggct gcgggaatgt ctcccgctaa
    21661 cgcgctggca accaataaac tgcaagcctg cggcggctct atttccgcca ctatctactt
    21721 tattcgccgc aaagtggtta gtttaagcga tcagaaactc aatattgcca tgacctttgt
    21781 cggctcaatg agcggcgcac tgttggtaca gtacgttcag gctgatgtct tacggcagat
    21841 tttgcccatt ctggtgattt gtatcggcct ctatttcttg ctgatgccaa agctgggtga
    21901 agaagatcgc cagcgccgga tgtacggttt acccttcgcg ttaattgccg gtggctgcgt
    21961 cggtttttat gacggattct ttggcccggc tgccggatcg ttttacgcgc tggccttcgt
    22021 tatgctgtgc ggattcaacc tcgccaaagc cacggctcac gccaaattac tcaacgcaac
    22081 gtcaaacatt ggcggtctgc tgctgtttat tctcggcggc aaagtgattt gggcgacggg
    22141 ctttgtcatg ctggtggggc agttcctcgg cgcgcgcatg ggttcacggt tggtgttgag
    22201 caaaggacaa aagctgatcc gcccgatgat tgttatcgtc tcggcggtga tgagtgccaa
    22261 attactttac gatagccacg gacaggagat cctccactgg ttggggatga actaatgaac
    22321 agtacacacc actacgagca gttgattgaa atttttaata gctgctttgc cgatgaattt
    22381 aatacccgtc tgattaaagg cgacgacgaa ccgatctatc ttcctgctga tgcggaagta
    22441 ccgtataacc gaatcgtctt tgctcatggc ttttatgcca gcgcaattca tgagatttcg
    22501 cactggtgta ttgccggaaa agcgcgccgt gagctggttg atttcggcta ctggtattgc
    22561 ccggacggac gcgatgccca gacgcaaagc cagtttgaag atgttgaagt gaagccgcag
    22621 gcgttcgact ggttattctg cgtggcggct ggatatccgt ttaacgtcag ttgcgacaat
    22681 ctggaagggg atttcgaacc ggatcgtgtg gtgtttcaac ggcgggtaca tgcgcaggtg
    22741 atggattatc tgacaaatgg cattccggaa cgcccggcac gcttcattaa agcactacaa
    22801 aattattatc acacgccgga acttacggcg gaacagttcc cgtggccgga agcgctcaac
    22861 tgaagtcagc gccacgtttt acaatgagga aagaagatga tcgcggagtt tgaatcacgc
    22921 attctggcat taatcgacgg catggttgac catgccagtg atgatgagtt gtttgccagt
    22981 gggtatttgc gtggccacct gacattagcc atcgcagaac tggaaagtgg tgatgaccac
    23041 tccgctcagg cggtgcatac gaccgttagc cagagtctgg aaaaagccat tggtgccggt
    23101 gaattgtcgc cgcgtgacca ggcgctggtg accgatatgt gggaaaacct gtttcagcag
    23161 gcgtcacagc tgtaagttgc ggcgtctgga gcaccatgcc tgatgcgacg cttatcgcgt
    23221 cttatcaggc ctacacgtca cacgtaggtt ggataaggcg ttcacgccgc atccggcaaa
    23281 cactgcgaga gcagattacc ccgccttaac cgctttaccc ttcaacaatt tccgcaccca
    23341 taaccgattc gggtttaacg ccgccagcgt actggcatcc atcggaatcg gttcgtcgct
    23401 catctgcgcc gccagaatct cggcacacag cggggcagaa cacaaaccgc gagaacctaa
    23461 agccgcaaac ataaagagat cgtcaaaaac cggcgcgctt accgcctcat ctttttgctc
    23521 cgccaacgac gcatattcca cgagtgttgc ctcataatcg ggaacattgc ctaccattgg
    23581 cagatgatcg cgggtggcac aacgcacacc gcagcgcgcc tctttatcac tgacatcaac
    23641 ctcttttgcc cactgtgcct gcgggaaaca atcaatcaac cgctggcgat tctgctgctg
    23701 atcgtcctca ctgtacgccg tatcttcgct gccgcgatga taactggcac caatacaatg
    23761 atgttgattc gccggatttt gtggcgtgag ataaccgtca tagcacagca cctgcttcag
    23821 ctctgccagt tccggcgttg tcggaatatg gctgacctgc ccggcaaccg aatacaccgg
    23881 gagagtcgac gtttggctga aacggctgat ttgatgcccg ttcgccagta ccactacgct
    23941 gtgtgttgct tgctgatctc ctgcaaaatt caacaaccaa cagtcatcct tacgggataa
    24001 attctgtaac tgatattgat aataaatctg caaaccctgc tgttgcgcca gttccagcac
    24061 attacgggtc agttctgctg ggcacagcca accaccttgc ggataagtaa tgccgctgca
    24121 atttgtcgca acgcccgtaa tttgttcaac cgcatttgcc tcaacggcta cagccagttc
    24181 tgcgggtaaa tccattgaca acatctgtgc gattttatgc tggcttttct catcccagcc
    24241 taactgcgtg acgccgcacc agtcatgatc aaatttaacg ggtaattggt cgtaaaaccg
    24301 acgagcaaaa gtaaacgcat tagagaaaaa gcggtttagc gcctcatcgt gtttgcttaa
    24361 taacggatac agcgccccct ggcgattgcc ggaagcaccc agcgcggggg cttcatccgc
    24421 gcaataaagc gctacctgcc agccgcgccg taataacgcc agcgacaaca acgcgctggc
    24481 aataccaccg ccgataatcg ctacttcccg tttgctgctg cccgtacgat taaaccacgg
    24541 cgtggagcag gggagtggta atgtctgttc catcacccca caaagcattt cccgtttgcg
    24601 cccaaagccc ttacgttttt gcatcgtgaa tccggcttcc tgcaaaccgc ggcggacaaa
    24661 accggcagac gtaaatgtcg ccagcgtgcc gcctggacgc gccaaccttg ccatggcgtt
    24721 aaacagattt tgcgtccaca tatccgggtt tttagctggc gcaaagccgt ccagaaacca
    24781 ggcatctact ttttgattta gcgaatcgtc cagttggctg gtcagttcgt taatatcgcc
    24841 aaaccataaa tccagcgtca cgcggccttc atcgagcaat aaacgatggc aaccgggcaa
    24901 gggcattggc cactgcgcct gaagttgttc tgcccacgga gccagttccg gccagtgttg
    24961 atgcgctaag gctaaatccg cacgggtgag gggaaatttc tcaaaactaa tgaaatgtaa
    25021 gcgttgtaat tgcgcttgcg gatgcgcttc gcgaaactga tcaaatgcct tccatagcgt
    25081 caggaagttt aatccggtgc cgaagccgct ctctgctatc acaaacagag gatgtggatg
    25141 ctcaggaaag cgtacctcta attggttacc tcccagaaaa acataacgcg tctcttccag
    25201 cccgttatcg ttggaaaaat agacatcgtc aaaatctcgg gaaacaggtg taccctcagc
    25261 attaaattcg aggttggcag gttgtatgga gtagtgtttc acgtaagtta ctcgtcttac
    25321 aggcggtggc tcgatcttag cgatgtgtgt aaggctgcgc aaatatctct attaaatggc
    25381 tgatcggact tgttcggcgt acaagtgtac gctattgtgc attcgaaact tactctatgt
    25441 gcgacttaca gaggtattga atgaaacgtg cagtgattac tggcctgggc attgtttcca
    25501 gcatcggtaa taaccagcag gaagtcctgg catctctgcg tgaaggacgt tcagggatca
    25561 ctttctctca ggagctgaag gattccggca tgcgtagcca cgtctggggc aacataaaac
    25621 tggataccac tggcctcatt gaccgcaaag ttgtgcgctt tatgagcgac gcatccattt
    25681 atgcattcct ttctatggag caggcaatcg ctgatgcggg cctctctccg gaagcttacc
    25741 agaataaccc gcgcgttggc ctgattgcag gttccggcgg cggttccccg cgtttccagg
    25801 tgttcggcgc tgacgcaatg cgcggcccgc gcggcctgaa agcggttggc ccgtatgtgg
    25861 tcactaaagc gatggcatcc ggcgtttctg cctgtctcgc cacgccgttt aaaattcatg
    25921 gcgttaacta ctccatcagc tccgcgtgtg cgacttccgc acactgtatc ggtaacgcag
    25981 tagagcagat ccaactgggc aaacaggaca tcgtgtttgc tggcggcggc gaagagctgt
    26041 gctgggaaat ggcctgcgaa ttcgacgcga tgggtgcgct atctactaaa tacaacgaca
    26101 ccccggaaaa agcctcccgt acttacgacg ctcaccgtga cggtttcgtt atcgctggcg
    26161 gcggcggtat ggtagtggtt gaagagctgg aacacgcgct ggcgcgtggt gcttacatct
    26221 atgctgaaat cgttggctac ggcgcaacct ctgatggtgc agacatggtt gctccgtctg
    26281 gcgaaggcgc agtacgttgt atgcagatgg cgatgcatgg cgttgatacc ccaatcgatt
    26341 acctgaactc tcacggtact tcgactccgg ttggcgacgt gaaagagctg gcagctatcc
    26401 gtgaagtgtt cggcgataag agcccggcga tttctgcaac caaagccatg accggtcact
    26461 cactgggcgc tgcgggtgtg caggaagcta tctactctct gctgatgctg gaacacggct
    26521 ttatcgcccc gagcatcaac attgaagagc tggacgagca ggctgcgggt ctgaacatcg
    26581 tgaccgaaac gaccgatcgc gaactgacca ctgttatgtc taacagcttc ggcttcggcg
    26641 gcaccaacgc cacgctggta atgcgcaagc tgaaaagatt aattcgcagt aggccggagt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacgttacg cgccaatgcg gcctccggca
    26761 ctaacgcaaa agggaacctg atggttccct ttttcacatc attgacaatc gccaccagtt
    26821 ctaggcaaac ttcccgcttt gtcgatttcc ttctgaaaag acgtacgcgt taaatcctgc
    26881 caacgcaccg taaccctgaa accagagaga tgagacgggg atactcctcg ccttgcgctg
    26941 cattctggag taatgcatga ctgctgtaag ccaaaccgaa acacgatctt ctgccagttt
    27001 ttcgctcttc cgcatctctt ttgcggtttt tctcacctac atgaccgtag ggttgccgtt
    27061 gccggttatc ccgctgtttg ttcatcatga tctgggctat ggcaatacca tggtcggcat
    27121 tgccgtcggg attcagtttc tggctacggt gctgacgcgt ggttacgccg ggcgactggc
    27181 cgatcaatat ggcgcaaaac gttcggcgct tcaggggatg ttagcttgtg gtctggctgg
    27241 cggcgcgttg ctgctggcgg cgattttgcc tgtctccgca ccgttcaaat ttgccctgtt
    27301 ggtcatcggg cgtttgattc ttgggtttgg tgaaagccag ttactgacag gcgctctgac
    27361 ctgggggtta ggcatcgtag ggccaaaaca ctctggcaaa gtgatgtcat ggaacggaat
    27421 ggcgatttac ggtgccctcg ctgttggtgc gccgcttggc ctgttgattc atagccatta
    27481 cggttttgcc gcactggcga tcaccacaat ggtattaccc ttactggcgt gggcctgtaa
    27541 cggcacagtg cgcaaagtac cggccctggc gggagaacgt ccatcgctgt ggagcgttgt
    27601 cgggcttatc tggaaaccag ggttagggct ggcactacaa ggcgttggtt ttgcggttat
    27661 cgggactttc gtttcgctct actttgccag caaaggatgg gcgatggcgg gctttactct
    27721 taccgcgttt ggcggcgcat ttgtcgtgat gcgcgtcatg tttggctgga tgccggaccg
    27781 ttttggcggc gtgaaagtgg cgattgtctc tctgcttgta gaaacggtgg gcttgttgct
    27841 gctctggcaa gccccaggtg catgggtcgc attagcgggc gcggcgttaa ccggagccgg
    27901 atgttcgctt atctttcctg cgctgggcgt ggaggtggtt aaacgcgtcc cctcacaagt
    27961 tcgcggcacc gcactgggcg gttacgccgc gtttcaggat atcgccctcg gcgtctccgg
    28021 gccgctggcg ggaatgctgg cgaccacgtt tggttactct tcggtatttc ttgccggggc
    28081 gatctctgcg gtgctgggta ttattgtcac gatactgtca tttcgtcggg gttaacgaac
    28141 cagccagacc agcatcagga taatcgcgac taacagtatc cacagcgcgg gacgcgttgc
    28201 catattttgc aacgtgtcca tcatcggtgg aaaaggatta agcagcggtt gcatatcacg
    28261 cggatcaatc cctttaaccc gccgttgata aagctgatta agaatatcct cggcctgggc
    28321 agaagaaagc gatgattgcg cagaaaggcc atatttttgc tggatatatg ccgataacgc
    28381 cgccagttca ctggcatcta aaggttgttt tagcgccatc tgtagtgatt ccagcgtcgg
    28441 cgtattttgc tggcttagcg tctgacgcgc ctgtagccag gtcaccagat ggttaaacag
    28501 tttcgctgga attaactcgc catctttcac cccggaaagt tccagcatcg attgccagat
    28561 ctgtttgctg ggttcccccg ttgccgccgc aagtttggtc accagctgtt ttagcgcatt
    28621 gtgctccgcc ggtaataaag gacggtcggt cgcctcgcgc tgctgtggtt gcggaataac
    28681 catcttccct tcctgcaaca gggtgagaat ggtttttaat tgctccggtg agagctgatt
    28741 cagcggcgtc tgaccaaagt tatgacggat ataatccgtg accgcctgac gatgattccc
    28801 cagacgtaaa tactccccta attgcgctaa aagctggcgg gcagaatggc ttttttgcgc
    28861 ggccagcaga cgttgcgcca gattatgctc agcggcaggg aagtgacgcg aaagcagcgg
    28921 tgaatctccc gacagaccaa tatcgtgcct gatacccgcc cacagttctg ctctttgttg
    28981 ttgcgtcagt gaggtcactt tcgtcattaa gctttccagc gaagtacgtt gctgactgga
    29041 taaaggctga ttgcccgcgc cagacggcag gttatctccc tgacctggtg gctgcccagg
    29101 aggagggccg gaaataggtt gtatcattac gtatccttat acctgaaatc ttcgcaagta
    29161 tgcctggccg cgagattatg gcatacttgt ccggttaact ctcgtctcat acaggtaaca
    29221 caaacgtgaa aatccttgtt gatgaaaata tgccttatgc ccgcgactta tttagccgtt
    29281 tgggtgaggt gatcgcggtt cccgggcgtc caatccccgt cgctcaactg gcagacgcgg
    29341 atgcgctgat ggtgcgttcg gtcacgaaag tgaatgaatc tttgctggca ggaaaaccca
    29401 ttaaatttgt tggaactgcc actgcgggga ccgaccatgt cgatgaagca tggttgaagc
    29461 aggcgggaat tggtttttcc gctgcacctg gctgtaatgc gattgcggtg gtggaatatg
    29521 ttttctcctc cctgctgatg cttgccgaac gcgatggatt ttcactgcac gaccgtacgg
    29581 tggggatcgt gggcgttggt aacgttggac gtcgattgca ggcgcgactg gaagcgttag
    29641 ggattaaaac cttactttgc gatccgcctc gcgccgaccg tggggatgag ggtgatttcc
    29701 gctcgctgga tgagttagtc cagcgcgcgg atattctgac tttccatacg ccactcttta
    29761 aagatggtcc gtacaaaacg ctacatctgg cggatgaaaa actgatccgt agcctgaagc
    29821 ccggagcgat tctgattaac gcctgccgtg gcgcagtcgt cgataatacc gcgctgctga
    29881 cctgcctgaa tgaaggccag aagttaagcg tggtgctgga tgtctgggaa ggcgagccgg
    29941 aacttaatgt ggaattgctg acaaaagtgg atatcggcac gccgcatatc gcaggctata
    30001 ccctggaggg taaagcgcgt ggcacgacgc aagtgtttga agcctatagc aagtttattg
    30061 ggcatgaaca acacgttgcg ctggatacgt tgctgcccgc gcctgagttt ggccgcatta
    30121 cgctgcatgg tccgctcgat caaccgacgc tgaaaaggct ggtgcatttg gtgtatgatg
    30181 tgcgccgcga tgacgcaccg ctgcgtaaag tcgccgggat accgggtgag ttcgataaac
    30241 tgcgcaaaaa ctatcttgag cgccgtgaat ggtcatctct gtatgtaatt tgtgatgacg
    30301 ccagtgcggc atcattgctg tgtaaactgg gttttaacgc cgttcatcat ccggcacgtt
    30361 aatctcttct tcatgctctc tgctgtaaca ttggcaggga gctttgctat ttctggagta
    30421 aaccaccatg tctgaaggct ggaacattgc cgtcctgggc gcaactggcg ctatgggcga
    30481 agccctgctt gaaacgctgg ctgaacgtca gttcccggtt ggggaaattt atgcactggc
    30541 acgtaacgaa agcgcaggcg aacaactgcg ctttggtggt aaaacaatca ccgtgcagga
    30601 tgccgctgaa ttcgactgga cgcaggcgca gctggcattt tttgtcgcag gcaaagaagc
    30661 taccgctgcc tgggttgaag aagcgaccaa ctcaggttgc ctggtgatcg acagcagcgg
    30721 actgtttgct ctcgaacccg acgtaccgct ggtggtgccg gaagtaaacc cgtttgtact
    30781 gaccgattac cgcaaccgga atgtcatcgc cgtaccagac agtctgacca gccagctgct
    30841 ggcggcactg aaaccgttaa tcgatcaggg cggtttgtcg cgtatcagtg ttaccagcct
    30901 gatttcagcc tccgcccagg gcaaaaaagc ggtcgatgcg ttagcggggc agagtgcgaa
    30961 attgctcaac ggcattccga ttgacgaaga agatttcttc ggtcgtcaac tggcgttcaa
    31021 tatgctgccg ttgttgccgg atagcgaagg tagcgtgcgt gaagaacgtc gtatcgttga
    31081 cgaagtacgc aaaatcctgc aggacgaagg gctgatgatt tcggcaagcg tcgtccaggc
    31141 accggtattc tacggtcatg cccagatggt caactttgaa gcactgcgtc cgctggcggc
    31201 agaagaagcg cgtgatgcgt ttgctcaggg cgaagatatt gtgctctctg aagagaacga
    31261 attcccgact caggtagggg atgcttcggg tacgccgcat ctttctgttg gctgcgtgcg
    31321 taatgactac ggtatgccgg agcaagtcca gttctggtcg gtggccgata acgttcgctt
    31381 tggcggcgcg ctgatggcag taaaaatcgc cgagaaactg gtgcaggagt atctgtacta
    31441 atgtccgacc agcaacaact gccagtttat aaaattgcgc tgggcattga gtacgacggc
    31501 agtaagtatt acggctggca acggcagaat gaagtccgca gtgtgcagga gaagctggaa
    31561 aaggcgctct cccaggtggc gaacgaaccc atcaccgtct tctgcgccgg gcgtactgac
    31621 gcaggggtac acggtaccgg gcaagtggtg catttcgaaa caaccgcgca gcgcaaagac
    31681 gcggcgtgga ccttgggcgt aaatgcgaat ttacctggtg acatcgccgt gcgttgggtt
    31741 aaagctgtac ctgatgattt tcatgcacga tttagcgcca cggctcgccg ttatcgctac
    31801 atcatctaca atcatcggct gcgcccggcg gtactgagta aaggggtaac ccatttttac
    31861 gaaccgctgg acgctgaacg gatgcatcgg gctgcgcaat gcttgctggg cgagaatgat
    31921 ttcacctcgt tccgtgcggt gcagtgccag tcccgaaccc cgtggcgcaa cgttatgcat
    31981 attaatgtca cgcgtcacgg tccttatgtg gtggttgata tcaaagcgaa tgcctttgta
    32041 catcatatgg tcaggaatat tgtcggcagc ctgatggaag tcggtgccca caaccagccg
    32101 gagagctgga tagcagagtt gctggcggca aaggacagaa cgctggcggc agcaacggca
    32161 aaagcggaag ggctgtatct ggtcgcggtg gattaccctg accggtatga tcttccaaaa
    32221 ccgccaatgg gcccgctatt tctggcggac taacgagaat aatgctcgga acatttcagg
    32281 ctgtagccaa cgtagttaca gcctgaaaga tgacgagtac aaggcatagg caaatatgga
    32341 cctgatttat ttcctcatcg attttatcct gcacattgac gtgcatctgg cagagctagt
    32401 cgcggagtac ggcgtctggg tttatgccat tttgttctta attttgttct gtgaaaccgg
    32461 tctggtggta atgccgtttt taccgggtga ttcattgctg tttgttgccg gagcgttggc
    32521 atcactggaa accaacgatc tcaacgtcca tatgatggta gtgctgatgc tgatcgccgc
    32581 gattgtgggt gacgcggtca attacactat cgggcggttg ttcggtgaaa agttattcag
    32641 taatcccaat tcgaaaattt tccgtcgcag ttatctcgac aaaacccatc agttttatga
    32701 gaaacatggc ggcaaaacga ttattctcgc ccgttttgtg ccgatcgtca gaacgttcgc
    32761 cccgtttgtt gcgggaatgg gccacatgtc gtaccgtcat ttcgctgctt ataacgtgat
    32821 cggcgcactg ttgtgggtac tgctttttac ctacgcaggc tatttcttcg gtacaatccc
    32881 gatggttcag gataacctta agctgctgat cgtcgggatt attgtggttt ctattttgcc
    32941 gggcgtcatc gaaataatcc gtcacaaacg cgctgcggca cgcgccgcaa aataaaaagt
    33001 aactccgcgg ttcgaccact tttttatcca aagtttcggg ctgttatgtt ttaatgtgca
    33061 acattcatgg tctgttgggg gcaaaaatgg cattatgcgt ccccaaagat aaaactggca
    33121 tcgaaccagg ttcagacaga aaggtcccta atgagctgga ttgaacgaat taaaagcaac
    33181 attactccca cccgcaaggc gagcattcct gaaggggtgt ggactaagtg tgatagctgc
    33241 ggtcaggttt tataccgcgc tgagctggaa cgtaatcttg aggtctgtcc gaagtgtgac
    33301 catcacatgc gtatgacagc gcgtaatcgc ctgcatagcc tgttagatga aggaagcctt
    33361 gtggagctgg gtagcgagct tgagccgaaa gatgagctga agtttcgtga ctccaagaag
    33421 tataaagacc gtctggcatc tgcgcagaaa gaaaccggcg aaaaagatgc gctggtggtg
    33481 atgaaaggca ctctgtatgg aatgccggtt gtcgctgcgg cattcgagtt cgcctttatg
    33541 ggcggttcaa tggggtctgt tgtgggtgca cgtttcgtgc gtgccgttga gcaggcgcag
    33601 gaagataact gcccgctgat ctgcttctcc gcctctggtg gcgcacgtat gcaggaagca
    33661 ctgatgtcgc tgatgcagat ggcgaaaacc tctgcggcac tggcaaaaat gcaggagcgc
    33721 ggcttgccgt acatctccgt gctgaccgac ccgacgatgg gcggtgtttc tgcaagcttc
    33781 gccatgctgg gcgatctcaa catcgctgaa cctaaagcgt taatcggctt tgccggtccg
    33841 cgtgttatcg aacagaccgt tcgcgaaaaa ctgccgccta gattccagcg cagtgaattc
    33901 ctgatcgaga aaggcgcgat cgacatgatc gtccgtcgtc cggaaatgcg cctgaaactg
    33961 gcgagcattc tggcgaagtt gatgaatctg ccagcgccga atcctgaagc gccgcgtgaa
    34021 ggcgtagtgg tacccccggt accggatcag gaacctgagg cctgataact gataagggca
    34081 gggccactgg ctctgccctt ttgctattct caccgtaacg aatcagcgga taccatgatt
    34141 atcaaacgca ctcctcaagc cgcgtcgcct ctggcttcgt ggctttctta tctggaaaac
    34201 ctgcacagta aaactatcga tctcggcctt gagcgcgtga gccaggtcgc ggcgcgtctt
    34261 ggcgtcctga aaccagcgcc atttgtgttt accgttgcgg gtacgaatgg caaaggcacc
    34321 acctgccgta cgctggagtc gattctgatg gcggcagggt acaaagtggg cgtctacagt
    34381 tcgcctcatc tggtgcgtta taccgagcgc gtacgtgtgc agggccagga attgccggaa
    34441 tcggcccaca ccgcctcttt tgcggagatt gaatcggcac gcggtgatat ttccctgacc
    34501 tatttcgagt acggtacgct gtcggcgttg tggctgttca agcaggcaca acttgacgtg
    34561 gtgattctgg aagtagggct gggcggtcgt ctggacgcaa ccaatattgt cgatgccgat
    34621 gtcgcggtag taaccagtat tgcgctggat cataccgact ggctgggtcc agatcgcgaa
    34681 agtattggtc gcgagaaagc aggcatcttc cgcagcgaaa aaccggcaat tgtcggtgag
    34741 ccggaaatgc cttctaccat tgctgatgtg gcgcaggaaa aaggtgcact gttacaacgt
    34801 cggggcgttg agtggaacta ttccgtcacc gatcatgact gggcgtttag cgatgctcac
    34861 ggcacgctgg aaaatctgcc gttgccgctt gtcccgcaac cgaatgccgc aacggcgctg
    34921 gcggcactgc gtgccagcgg gctggaagtc agtgaaaatg ccattcgcga cgggattgcc
    34981 agcgcaattt tgccgggacg tttccagatt gtgagcgagt cgccacgcgt tatttttgat
    35041 gtcgcgcata atccacatgc ggcggaatat ctcaccgggc gtatgaaagc gctaccgaaa
    35101 aacgggcgcg tgctggcggt tatcggtatg ctacatgata aagatattgc cggaactctg
    35161 gcctggttga aaagcgtggt tgatgactgg tattgtgcgc cactggaagg gccgcgcggt
    35221 gccacggcag aacaactgct tgagcatttg ggtaacggca aatcatttga tagcgttgcg
    35281 caggcatggg atgccgcaat ggcggacgct aaagcggaag acaccgtgct ggtgtgtggt
    35341 tctttccaca cggtcgcaca tgtcatggaa gtgattgacg cgaggagaag cggtggcaag
    35401 taagtttcag aatcggttag tgggcacgat cgtgctggtg gcgctggggg tgattgtact
    35461 tccagggctg ctggacgggc agaaaaaaca ttatcaggat gagttcgcgg ctatcccgct
    35521 ggtgccgaaa gcgggcgatc gtgatgagcc tgatatgatg ccagccgcca cccaggcgtt
    35581 gccgacgcag ccgccggaag gcgcagcgga agaggtgcgg gcagg
//
LOCUS       DADLCQ010000030        34946 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000030 DADLCQ010000000
VERSION     DADLCQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.030"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..2261
                     /locus_tag="JQG95_001330"
     CDS             <1..2261
                     /locus_tag="JQG95_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5887948.1"
                     /translation="AKSLYLNPDKNTVNAFIHQQDINVLAQDDYQSLSIQGNGALGIT
                     ENSYIGAHWNFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGGN
                     FTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFYL
                     NSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTT
                     SQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISA
                     SVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISAT
                     VSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAI
                     VMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYS
                     EQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSD
                     SYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQD
                     LQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNREL
                     MLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGS
                     EKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLR
                     GNVQTAQREVAP"
     gene            2714..3346
                     /locus_tag="JQG95_001331"
     CDS             2714..3346
                     /locus_tag="JQG95_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY5887949.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3434..4732)
                     /locus_tag="JQG95_001332"
     CDS             complement(3434..4732)
                     /locus_tag="JQG95_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5887950.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5216..6628
                     /gene="uxaC"
                     /locus_tag="JQG95_001333"
     CDS             5216..6628
                     /gene="uxaC"
                     /locus_tag="JQG95_001333"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY5887951.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6643..8130
                     /locus_tag="JQG95_001334"
     CDS             6643..8130
                     /locus_tag="JQG95_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY5887952.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8213..8764
                     /locus_tag="JQG95_001335"
     CDS             8213..8764
                     /locus_tag="JQG95_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY5887953.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="JQG95_001336"
     CDS             complement(8769..10013)
                     /gene="sstT"
                     /locus_tag="JQG95_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY5887954.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10609..11574)
                     /gene="alx"
                     /locus_tag="JQG95_001337"
     CDS             complement(10609..11574)
                     /gene="alx"
                     /locus_tag="JQG95_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY5887955.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11845..12861)
                     /locus_tag="JQG95_001338"
     CDS             complement(11845..12861)
                     /locus_tag="JQG95_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY5887956.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(12922..13614)
                     /locus_tag="JQG95_001339"
     CDS             complement(12922..13614)
                     /locus_tag="JQG95_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY5887957.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13687..14190)
                     /locus_tag="JQG95_001340"
     CDS             complement(13687..14190)
                     /locus_tag="JQG95_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5887958.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14275..15411
                     /gene="rlmG"
                     /locus_tag="JQG95_001341"
     CDS             14275..15411
                     /gene="rlmG"
                     /locus_tag="JQG95_001341"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY5887959.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15696..16010
                     /locus_tag="JQG95_001342"
     CDS             15696..16010
                     /locus_tag="JQG95_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY5887960.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16007..16423
                     /gene="higA"
                     /locus_tag="JQG95_001343"
     CDS             16007..16423
                     /gene="higA"
                     /locus_tag="JQG95_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY5887961.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16468..18486)
                     /locus_tag="JQG95_001344"
     CDS             complement(16468..18486)
                     /locus_tag="JQG95_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY5887962.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTILLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18712..21071)
                     /gene="ygjK"
                     /locus_tag="JQG95_001345"
                     /pseudo
     CDS             complement(18712..21071)
                     /gene="ygjK"
                     /locus_tag="JQG95_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(21088..22159)
                     /gene="ygjJ"
                     /locus_tag="JQG95_001346"
                     /pseudo
     CDS             complement(21088..22159)
                     /gene="ygjJ"
                     /locus_tag="JQG95_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(22293..23726)
                     /locus_tag="JQG95_001347"
     CDS             complement(22293..23726)
                     /locus_tag="JQG95_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5887963.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23789..24238)
                     /locus_tag="JQG95_001348"
     CDS             complement(23789..24238)
                     /locus_tag="JQG95_001348"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY5887964.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24235..27312)
                     /gene="ebgA"
                     /locus_tag="JQG95_001349"
     CDS             complement(24235..27312)
                     /gene="ebgA"
                     /locus_tag="JQG95_001349"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY5887965.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLDDYT
                     LHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTRYSE
                     AGHSIASYQFPLKENTAQPVPFAPNNVRPLTLEDDRLSCTVRGYNFAITFSKMSGKPT
                     SCQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSDGEV
                     LIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMGING
                     EYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTALTN
                     RHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSNSWG
                     SEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27494..28477)
                     /gene="ebgR"
                     /locus_tag="JQG95_001350"
     CDS             complement(27494..28477)
                     /gene="ebgR"
                     /locus_tag="JQG95_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY5887966.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28695..29026
                     /locus_tag="JQG95_001351"
                     /pseudo
     CDS             28695..29026
                     /locus_tag="JQG95_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(29068..30558)
                     /gene="ygjG"
                     /locus_tag="JQG95_001352"
     CDS             complement(29068..30558)
                     /gene="ygjG"
                     /locus_tag="JQG95_001352"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY5887967.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            30865..32385
                     /locus_tag="JQG95_001353"
                     /pseudo
     CDS             30865..32385
                     /locus_tag="JQG95_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(32539..33162)
                     /locus_tag="JQG95_001354"
     CDS             complement(32539..33162)
                     /locus_tag="JQG95_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY5887968.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33439..34184
                     /gene="nfeF"
                     /locus_tag="JQG95_001355"
                     /pseudo
     CDS             33439..34184
                     /gene="nfeF"
                     /locus_tag="JQG95_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34238..34313)
                     /locus_tag="JQG95_001356"
     tRNA            complement(34238..34313)
                     /locus_tag="JQG95_001356"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34278..34280),aa:Ile,seq:cat)
     gene            34438..34944
                     /gene="mug"
                     /locus_tag="JQG95_001357"
                     /pseudo
     CDS             34438..34944
                     /gene="mug"
                     /locus_tag="JQG95_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8541 a         8974 c         9177 g         8254 t
ORIGIN      
        1 atgcgaagtc attatattta aatccagaca aaaatactgt taacgctttt atacatcagc
       61 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
      121 cgctgggaat aacagaaaat agctatattg gtgcacactg gaatttcaac ggttatgatg
      181 cagatgatgt cagtgacagt aatactgacg tcagcgatct ctattatcgt tatgattttt
      241 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
      301 gcgggaactt tacctttaac tttctgccac tcggtgcaat cgatgggatg cgtatcgggt
      361 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
      421 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
      481 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg
      541 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
      601 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
      661 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
      721 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
      781 ataactggac ggcagattta ggcagggtgg ggaatcttgc aatcagcgcc agcgtgttcc
      841 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
      901 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
      961 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacagtttca ctgaattttg
     1021 tcggctggaa tatgatgctg ggttatacct gcacacaaaa taacactgat gatagtttgc
     1081 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
     1141 tctctgaaac tgtacaactt agcgcttccc gcgctattgt gatgcgtgac tggattttga
     1201 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
     1261 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt
     1321 caaccaatgt gcctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
     1381 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
     1441 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
     1501 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
     1561 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
     1621 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac
     1681 aggatgaaga gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
     1741 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
     1801 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
     1861 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
     1921 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
     1981 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
     2041 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
     2101 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
     2161 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
     2221 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
     2281 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
     2341 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
     2401 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
     2461 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
     2521 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
     2581 gctaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
     2641 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
     2701 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
     2761 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
     2821 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
     2881 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
     2941 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
     3001 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
     3061 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
     3121 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
     3181 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
     3241 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
     3301 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
     3361 caaacgcttt tttctatata aacgacgaag ccgcctgata aggcggcttc gtcgttataa
     3421 cttacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     3481 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     3541 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     3601 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     3661 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     3721 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     3781 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     3841 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     3901 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     3961 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcaa
     4021 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4081 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4141 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4201 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4261 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
     4321 agcctactgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
     4381 ccgggatcat cgctgcttcc gcggcaccga ccgcaccacg agcaacagct aggccacccc
     4441 agctacctgc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
     4501 aaccgatttt cgtacccagc acatccagta cataacctgc taccggttgc atgaccgtat
     4561 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
     4621 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
     4681 cgagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
     4741 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
     4801 tagttacgac cgggtaaccg aaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     4861 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg aatgaatcgg
     4921 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaacc
     4981 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     5041 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttgagagta atttttttaa
     5101 ctacgtttat tgatctaact caagaaaata tcttcggact ctggaaattg gtgtgataac
     5161 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     5221 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     5281 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     5341 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     5401 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     5461 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     5521 ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact ggcaaattgc tttctccgtc
     5581 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     5641 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     5701 ttctctggag catcatgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     5761 gagctggcgt ccggacaaag ccttcaatat cgaacaggcg acctttaacg actacatggc
     5821 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     5881 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc atgcgctgga
     5941 cgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgccgtct
     6001 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tactggtgtt
     6061 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     6121 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     6181 cgaccgtccg atggcggaag agttgtctaa gctgctgagc aagcagaacg aagaaaacct
     6241 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     6301 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     6361 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggtct
     6421 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     6481 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     6541 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     6601 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     6661 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg ctgaaggcac agaagtcagt
     6721 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     6781 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     6841 gcggatattg cggcggggga acacgtccac gcccacaata cgcgcacgaa tctgagcgat
     6901 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcct
     6961 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaacga gctgtggatc
     7021 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa tccagaaccg tttcctgaaa
     7081 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctatggctgc
     7141 tcacaactgg gcgacgatca catcaacacc cgcaccatgc tgcaaaacat ggtgcgccac
     7201 ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg aaaacaacca ggttgccgca
     7261 ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
     7321 caggatgatg agatcgaagc cgggatcgag catttgcatc agctgtataa cgtgatgcgc
     7381 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
     7441 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
     7501 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
     7561 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac catggtcaac
     7621 gacttcaaac agtactttat tgcccatgat cagccgatct acgaaaaccc atcaccgggg
     7681 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
     7741 ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccagggctg
     7801 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
     7861 tgccatatgg tgctgttcag tactggtcgc ggcacgccat acggtggatt tgtgccgacg
     7921 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
     7981 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
     8041 atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc gtaacgactt ccgcgaactg
     8101 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
     8161 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
     8221 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
     8281 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
     8341 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
     8401 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
     8461 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
     8521 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
     8581 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
     8641 gattgagggt agttttctgc ttatgaatgg cctgaatatc attcgtttct ggcggatgca
     8701 gaaaagggga attgatccgt ttaaagtaga gaaaacccct cccgccatag acgaaagggg
     8761 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttccgc ctggcaagct
     8821 gccgcagtga acagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
     8881 acgccgataa taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
     8941 atattacagg ccagtgggat cagcagcaaa gaaccccccg ccacgccgga tgcgccacag
     9001 gcacacagag aagccactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
     9061 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
     9121 atggtggctc ccagcggaat agaaactgaa taggtatcgc gatccagatt cagcttttca
     9181 cacagcgcca tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca
     9241 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
     9301 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
     9361 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
     9421 ccaaaaatac cgaacggtgc gaagcgaatg accagtttca ccataaaggt aacggcattc
     9481 gacatatcat tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaagccgagg
     9541 ccgatcgccc acaccagaat cccgatgtag ttacctttca gcagggcgtc gatggggttg
     9601 gaaaccatgc tcattaccag tccgcgcatc acttcgacaa tgcctgacgg cggcgaaata
     9661 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
     9721 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
     9781 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
     9841 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
     9901 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
     9961 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    10021 tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    10081 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    10141 tggattttaa agagaggtca tgttttgcaa cttaatggtt gcatttattt aacataaaca
    10201 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    10261 tgagaacggt tgcgctcctt taacgtgtta tgccatataa agtcactggc gcagggagcg
    10321 cgcagggggc ggccaatcgc cgccgccccc tgcaccccca ggctctggcg aacaaaatcg
    10381 ccgctgcgcg gttccctcag cctatccctt gcggctaccg ggtcgggcgc aaggtaacgt
    10441 ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac
    10501 caacacccgt gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac
    10561 ctttgtctgg aatgagttat ctctaggaat ggcttaatat acagcgaatc attctccccg
    10621 ctgcttatca tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc
    10681 aaacaccacg cccagcgaga cggcgattgg aatatggtag aagtcgacaa tcagcatctt
    10741 gataccgata aacaccagaa tcaccgccag gccatatttg agcatcgaga aacgctctgc
    10801 tacgcccgcc agcaggaaat acatcgcacg caggccgagg attgcaaaca ggtttgaggt
    10861 cagcacaatg aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcaaa
    10921 aatcacgtcg ctcaattcca ccagaatcag taccagcatc agcggtgtgg cgtacagcaa
    10981 tccattctta cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc
    11041 gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggcgagcgc
    11101 catcttcacg ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg
    11161 cgaaatcagc cagctgcctg tgaagatcat gatggtacgc agaacaatcg ccccgagcac
    11221 gccatacacc agcacgcgac gttgtaatgc cgccggaacg gagaaatagc tgaacaacat
    11281 cagccagaca aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa
    11341 ggccagggcc tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa
    11401 agcggcgtta aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt
    11461 catggtcatg gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat
    11521 ggcgacaaca acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt
    11581 tccttacaca taaaaaaacg gccaacgtcg gaagacgtca gccgctgctt tttatgcata
    11641 gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct gcgtattcct
    11701 ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga tcgaccggca
    11761 atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc agtcaatggt
    11821 ctgttacata ccttttacac tgttttactt tggcaagtat agttttacgc tatctgccgg
    11881 aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt ttggctgtga tgcgactgac
    11941 cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca gctctgcttc
    12001 gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc tgccacgcgg
    12061 cacaaagcag actttctggc attcagacag tttttcaatc accagcgatc ccgcttcgcc
    12121 ctgaatctcg cttcccagga cagaatcact gacctttgag tgctgcaagg tgacgctgaa
    12181 atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg ccgcttgcca gcaaactcgc
    12241 cgttgcctgc acgcttttcg gctcaccaaa taacgccacc gccgacgcca ggcagtaaaa
    12301 gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg gattctcacc
    12361 gtccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga ctttacgcag
    12421 tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag ttcggcaggc aggcggtttt
    12481 aaatgcctca aacagcacca cctgattttc gcgcgcacag gcaatggcgg catccacttc
    12541 cgccagattc gacgccagcg gtttttcgca aatcacatga attttatggc taaggaaaag
    12601 ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt caatggcatc
    12661 gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg acagaaaaat cattggcgaa
    12721 gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt aacttgtatt taccgctctc
    12781 atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag cgaaacgtat
    12841 cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc ctttttcatc
    12901 ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac taaacggccc
    12961 aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggatttttg cgtcaatcac
    13021 cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct tcatattgag
    13081 gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc agatggcgcg
    13141 ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca cctttttggc
    13201 acgcactacc gaatccagcg ttgagaatcc ggcatagtcg caaaagatca ctttcgccgg
    13261 tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat ttttacgtcc
    13321 gttatcgcca ctcaccagta gccatttcac ttttccggcg tggtacaacg ctgctgcggt
    13381 atcaatacgt cgggtaaagt agcgattacc cggcctcgcc cccagcaata aacccacgtt
    13441 acgcgccggg acggcgttga catcgcccca ggtcagctgc ttgctggcgt tgaccataac
    13501 gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg caatctttag
    13561 agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca gcatggtcgt
    13621 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaaagata acgcctgaca
    13681 tcattttcag attttgtcct gcccaagcga caactgcgtt agccacagcc gggtgtcgaa
    13741 ctcaagctgg tggtactgcg gctccatatg gcagcacaat tgatagaacg ctttgttatg
    13801 ctctttctcc ttcaggtgcg ccagctcgtg caccacgatc atgcgcaaaa acggttccgg
    13861 cgcgttgcga aacacggtag cgacgcggat ctctgctttt gctttcagct tgccgccctg
    13921 cacacgagaa acagcggtat gtaacccgag cgcgtttttt agcacatgga tcttattgtc
    13981 atacatcact ttattgatcg gcggggcatt acgcagaaac tgatttttca gatcctgagt
    14041 atattgccag agagctttat cggtggcgta gtcgtgtgtt cccggatagc gttttgccag
    14101 cacatcgccc agacgctgtt cgttaatcag tgttcgcacc tgcgaaagca gctgctcggg
    14161 ataaccctga agataagtaa gattgctcat aaccgcccac gaaaaagaga aaaagggtat
    14221 actcacgcac ccttttcagg ggcaacgtcg aaattttatc attcaggagg gccgatgagc
    14281 cacttagaca acggtttccg ttcactgaca ctacaacgtt ttccggcgac ggatgacgtt
    14341 aacccgctac aggcgtggga agcggcagat gaatatttgc tgcaacagtt ggacgacaca
    14401 gaaatccccg gcccggtgtt gatcctgaat gatgcctttg gtgcgttaag ctgcgcgctg
    14461 gcggaacata agccgtacag cattggcgac tcatacatca gtgaactggc gacgcgcgag
    14521 aatttacgcc taaacgggat tgatgagtcg agcgtgaagt ttctcgacag caccgccgac
    14581 tacccgcaac agccgggtgt ggtgctgatc aaagtgccga aaacactggc attgctggaa
    14641 cagcaactgc gtgcgctgcg caaagtggtc acgtcggata cacgtattat tgccggtgcc
    14701 aaagcccgtg acattcacac ttccacgctg gaactgttcg aaaaagtgct cggcccgacc
    14761 accaccacgc tggcatggaa aaaagcgcgc ctgattaatt gcacgttcaa tgaaccgccg
    14821 ctggccgatg cgccgcagac cgttagctgg aagctggaag gtactgactg gactatccat
    14881 aaccatgcga atgtcttctc ccgcaccggg cttgatatcg gcgcgcgctt ctttatgcaa
    14941 catctgccag agaatctcga aggggagatt gtcgatctcg gttgcggtaa tggcgttatt
    15001 ggtctgacgc tgcttgataa aaacccgcag gcgaaagtgg tgtttgtcga tgaatcgccg
    15061 atggcggttg cttccagccg tttgaacgtt gaaaccaaca tgccagaggc gttggatcgc
    15121 agcgagttta tgatcaacaa cgcgctctcc ggcgtggagc ctttccgctt taatgctgtg
    15181 ctctgcaacc ctccgtttca ccaacaacat gcgctgaccg ataacgtcgc ctgggagatg
    15241 ttccactacg cccgccgctg cctgaaaatc aacggcgagc tgtatatcgt tgccaaccgc
    15301 catctggatt acttccataa actgaagaaa attttcggca actgcaccac tattgcgacg
    15361 aataataaat ttgtggtgct gaaagcggtg aagctgggac gtcgtcggta agattgggag
    15421 ttacctgccg gatgcggcgt aaacgcctta tccggcctac gttcagcacg ttacccctag
    15481 gccggataag acgcggcaag cgtcgcatcc ggcaatgtct gctcagttct ggaatcgcgc
    15541 ttccataaaa caaaaaatcc ctgtcaccat cctctacatt ctcttgttta gcgtttttct
    15601 acgtttattc ttccgtcaca cagataaatt catccgttgc caatctgtca acggatgtta
    15661 tcatattgcc aatttgtcaa cggacgtgat gaagaatgca cctgataact caaaaagcat
    15721 tgaaagatgc tgcggaaaaa tacccgcaac ataaaacgga gttggtggct ctggggaaca
    15781 cgattgctaa gggatatttc aaaaaacctg agtcattaaa agcagtattc ccatctctgg
    15841 ataacttcaa atatctggat aagcattatg ttttcaatgt tgggggcaat gaattacgtg
    15901 ttgtagcaat ggtctttttt gaatcgcaaa agtgctacat acgtgaagtt atgacgcata
    15961 aagaatacga tttctttacc gctgttcatc gtactaaggg gaaaaaatga ttgcgattgc
    16021 cgacatcttg caagcaggag aaaagctaac tgctgtggca ccttttctgg cgggtattca
    16081 gaacgaggaa caatacaccc aggcgctgga actggtagat catctgctgc tcaacgatcc
    16141 tgaaaacccc ttgctggatc tggtgtgtgc caaaataacc gcgtgggaag aatcagcgcc
    16201 cgaatttgcg gaatttaatg ccatggctca agccatgcct ggcggtatag ccgtgattcg
    16261 tacccttatg gatcaatatg gtttaaccct ttccgatctg ccggaaattg gcagtaaatc
    16321 tatggtgtca cgcgttttga gcgggaagag gaaattaacg ctggaacacg ctaaaaaatt
    16381 ggcaacgcga ttcggcattt ctcccgcctt gtttattgat taacgcgacg tgccggatgc
    16441 gttgcatccg gcgcttcaaa taacgattta aatctccagc gccagccgtg ttccctgggc
    16501 aattgcccgt cgtgcgtcca gctccatagc cacatcgcag ccgccaatta aatgcacagt
    16561 tttcccgcta tcaatcagcg gttgcgccag cgcgcggttt ggctcttgcc ctgcgcagat
    16621 caccacattg tccactgcta atacctgcgt ttcgccgttg atcaccacat gcagcccgtc
    16681 atcgtcaatc ttctgataac ttacgcctgg gatcattttc acaccccgcg agagcaggat
    16741 ggtgcgatgg atccagccgg tggttttgcc taacccctgt cccggtttgc tggctttgcg
    16801 ctggagcatc acaatttgcc gtgggctacg cgggatctgc attccctgcg ggcttaagcc
    16861 accagcctgt tgtaggctgc tgtcgatccc ccactcatta cagaacctgg cgatattctg
    16921 gctggtggat tcgcccggct gacttaaata catcgccgta tcaaaaccaa tcccgccaca
    16981 accgatgatg gcaaccttgt tgcctaccgg cgctttgtcg cgcagtacat cgagataact
    17041 caataccttc ggatgatcga tcccgtcgat aggcggaaca cgcggcacga tcccactggc
    17101 gaggatcgtt tcatcgaacg cctgtaactg atccgccgtc acggtgtgat tgagtttcag
    17161 cgtcacgccc gtcacttcga tcatccggcg gtagtagcgc agcgtttcgt aaaactcctc
    17221 tttgccgggg atctgtttgg cgatattaaa ctgcccgccg atctcgctgt gcgcatcaaa
    17281 caatgttacc tgatgcccac gcgccgccgc gttaatggca aacgccagcc cagcaggtcc
    17341 agcgccgacc accgccagat tttttttctg cacggcggga aggattggca ttttggtttc
    17401 gtggcaggcg cgaggattca ccaggcacga ggtgactttg ccaacgaaga tctgatcgag
    17461 gcagccctgg ttgcagccaa tacaagtgtt gatctcatcg gctcgccctg attgcgcttt
    17521 tgacagcagt tccgcatccg cgagaaacgg tcgcgccatc gatactatat cggcatcgcc
    17581 gcgcgagaga atatcgtcgg caacctgcgg atcgttaatc cggttggtgg ttaccagcgg
    17641 cagcgagacg tggcctttca gtttgcgcgt gacccagcta aatgcgccgc gcggcacggg
    17701 cgtggcgatg gtcggaatcc gcgcttcgtg ccagcctatt ccggtgttga taatggtcgc
    17761 gcccgccgct tcaatggcct gcgccagttc taccgtttcg gcaaaagtgc cgccgccttc
    17821 gaccaggtcg agcatcgaca accggtaaat aataatgaag tcgttgccga cgcgttcgcg
    17881 caccgtacgc actacttcta cggcaaatcg catccggttg cggtaatcgc cgccccactg
    17941 gtcactacgc tgattggtgc gcagcgtcag aaattcgttg atcaaatacc cttcagagcc
    18001 catcacttcg acgccatcgt atccggcctc ccgcgccagt tgcgcgcagc gggcgaaatc
    18061 gtcgatcagt tgcaggatct cttcatgggt gagttcgtga ggaacgaaac ggttgatggg
    18121 ggcctgcaat gcggacggtg cgaccagatg cggttggtag ctgtagcgcc cggtatgcaa
    18181 aatttgcagg gcgattttgc cgccttcctg atgtacggct tcggtaatgg tgcgatggtg
    18241 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    18301 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    18361 cgcagccagc cgttcagcac cgtccgggta ttcctccagc ccggtgtgca ttgaacccat
    18421 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcgacgggta
    18481 gctcataaaa ttgtccagta tgtaaaataa ttgttatgta gtcggatgag ttctaattta
    18541 gccgtcgatg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    18601 tacgcccggt ggtaacgccg gatgcgacgc ttgccgtgtt ttatccggct tgggggattg
    18661 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gccgttgcaa attactgctt
    18721 acggaaaaaa tcgttataca acatatacaa atgagcggca ctccaggaga aatttggtgc
    18781 gccttgctgc gcgcctgtca gcgggttgta attctcctga attgggccat cggcggttaa
    18841 tcctttggcg tgcctgaaga acgtatccgc cagcttcagg gcatcatcac gataaccgta
    18901 acgctccatc cctttcagac caaaccagaa ctgatccacc catacgcgcc cgcgccagta
    18961 gatatcagcg ccaaaagccg gattggttaa cgccgccgtt cccagcggaa caaaggtgtt
    19021 gaactcttta ggatcgagca tcaccttcac caccgcgtcg gcattggcct gcgttgccgc
    19081 accgttaaac agcggcgacc agccttccgg ccctttaccg cgctcaacaa tcggtttgcc
    19141 cgcgcagccg ttcgccagtg gtttatcttc aatacgtacg tcatagtaga actgggtagt
    19201 cgggtcgaac atacaggtgt tgatgtagtc cgcgagctgc tgtgccaact ggcgatagcg
    19261 tttggcctct tctggcttac cgaggatggt tgccatctcc gccagataat ggttatcgct
    19321 gtacatatag ctggcctgat ccaccgactc ctgcaatagc gagtagccca gcaacgttcc
    19381 gtcctgactg cggttttcgg cgaatttcac cgtccagtcg ctacgtttgc cgccattagc
    19441 gacatattta tccaactgtt ctttgtcgat aaaaccaaag acggcggcat catcacggcc
    19501 cgattcccac gacgcagcga cctgtgccgg aatttccaga ctgtcgtact ggcctttctc
    19561 caccacgcgg gcgtagttgt tcagcccaga ctgcgtctct tctttatcgc cttttttcac
    19621 cgtaaacagc atctcgccgc tctcggtgtt gtgggctttg tcgcgggtcg cgccatattc
    19681 cggcaccccg ttgccgttat gatcgcggtt acgtaaccac cagtcgtgat aggccaccag
    19741 tttcgggtac atctctgcca accaggcttt atcctgggtg acgttgtata cttccatcac
    19801 cgaccaggcg gcaaggctgg gtttggtatt acgttcgttc cagttgccgc catcgccacc
    19861 acgctcgggg ctaaggttcc acgctatcag gtcggggaca aagcccacat cctgcggacg
    19921 cacgctgtcg ccaggctgga tctgccagga gaagaccgcg cggatattct ctttggcgat
    19981 atccggattg aaatgcgcca tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg
    20041 attgccggag aaccagcgtc cggtcaccga cggcgtgacg gtgttataat tcaccgcacc
    20101 gcccggtgag cgccagttac cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg
    20161 ttccggcgtc gcatccggat tggttaaccc tttcttcagg tattcttccc agcgttgctg
    20221 cgaggcggtg agataaaacg ccggacgcgc cagaatatcg cggatctgca tttgctcttt
    20281 gctaacttcc tgagcggtca gcagatggga ataggtggtg tagagcgtgg tcgaaccgtt
    20341 gatatgcgcc ttactggtaa agcgattacg gttgatttca gtctgcaccg gcagggattt
    20401 atgcacctga tattccgatt cgccggaggt cagcagatcc caggtggcgc gcactttgcc
    20461 aaaggtgact ttcaggccat cacgggtggc gctgattttg cgctgatagt cagggtattc
    20521 gccagcaatg gttttatcgg aaagcggttt cccttctttc gcttccagtt tttccagcag
    20581 ttcgccatcc cacaccagat ccagcggttt attgctgatg attttagttt ccagtagtga
    20641 cgtgcgcggc gtggcgaagc gcagagtcat ttcgacctgc acatcttttg ctgtcagttt
    20701 ttgcaccagc gcgccgggaa tactgtatgc ctccagcgtg aagttgactt tcttgccgtc
    20761 ttgccgtctt gccagacagt caggcggtcg aaattgctgg ccataaagtt gatgtattct
    20821 tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt tcgggccgtc tggcaacaga
    20881 tgaccatgcc aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg
    20941 taatccttca tgtactgcgg cgcaccagta cggttaatca cgtttttata attgtcggcg
    21001 ttagcggcat gagcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt
    21061 ttgattttca tgagattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg
    21121 gtcgctggga tcgttagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa
    21181 acgtccgccg ccatattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc
    21241 agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccttttaa
    21301 cgcttcatac atggcgctca ccatcatccc gctttcactt cccggtacgt ccagcccgtc
    21361 ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta ggggtaaaca ggcccgtgag
    21421 atagagttgt cctgtgccgt cggtaatttc cagaccattc atccaggtga tgccttcggc
    21481 aaggtcatac tggatgtaac cgttcatcca ttgccggata ggtgtatttg tcgtcgtagc
    21541 gatcgtactt gccaaaatgt agccaggctt tgctttcgtc atgatgtccg gcaggtgtgg
    21601 cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgcg gtcataatgt
    21661 cgcgggtatt gggaatgacg tagcccaaat cgggtgtgaa gtcgccccac cattgcaggt
    21721 catccagcga ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat
    21781 aaccgccata gaaacggtta atcccacctt cgaaaccacc ccagctatgg tcaggcacct
    21841 aggcattacc gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc
    21901 aggagccgta cttcatctcc aggccacctt caatataggt gccgtcgctg tatttatgtt
    21961 tattctcacc ctctaaatcc atatatccac caagccctaa ttcgccgtaa aaacgaaatg
    22021 aaggagaaga tgtcgaggta attgcgggtg tttttgtgtc gtcatgttct gccggacgtg
    22081 cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat gctctgcatg
    22141 gtgcagtgat taatttcatt gtgttaatcc atgagctaaa gaaaaagtac agccttttat
    22201 cgttgtaatt ataaaaatat aaaggcagaa actattttcc ctctcctcat ccaggaagag
    22261 ggaaaatgcc tgctcatttt ttattagtct gcttaatcaa cattgctggc tggcgtcgct
    22321 tcaatatggc gctcttcagc cgttaatccc tttatatatt tgctgtattt ccaccacgca
    22381 aagccgagga agataacaat accgccgacg ttataaaaaa tgatggtcag aatattcgcg
    22441 ccagtcggga atgtcgaagc gacaaaccct acggcaaata tcgcaatcag cattgaaaca
    22501 acgataatac cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg atctaatttg
    22561 gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg gaagcatgga cgctgcggcg
    22621 gtcatattaa taatggtatt catcagatcc tgcacggtat tggaaccgag catcgggata
    22681 atcatcagcg ggatgacgat cagaaactgg atccatgctg cgcgcgccgg aacgccgttt
    22741 tcgttaagtt cgacggtttt cttaccaaag atgccttccg ggatttcgga gaagaaaatt
    22801 ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca ttgcggtaaa ggacaccaga
    22861 ccgacaaagc gattcatcaa cgcttccggc agaccaaaat acgccgccat gccgtggaat
    22921 acctgcaccg atccgccggt aaatttcaac tctttgctgc tgacgaagac gttaatcagc
    22981 accgaggaga cggaatacag tacgccgata aaaatcccgg cgaggatgat cactttaacg
    23041 aacgatttcg aaccgccttt gacgtcgtta acgtacaccg cgacggactc cgcgccacct
    23101 gcggccataa agatccaggt ggtaacgccg aggaacgccc agttgaagtt cgggatcatc
    23161 gcgtcaacgg tgatcggatc ggcaggctgt acgccgccaa ccagtgccgt acctgccagt
    23221 aaaatgtagg agagcgttaa cagcagcatc agcgttgaag tgacggaggt aattggcccc
    23281 agcattttcg ccccgttggt ggaaacccag gtggagaagg cgaacagcac catactgata
    23341 acggtggtgg caaccggcgt cataatatat tcgtagccga ggaaggcata cgaagcataa
    23401 gcaataacgc gcggcaacag cgaggtgaaa aagaacagat ttacgaacca gtaagtgtag
    23461 gcagtaataa atgcccaacg tccgcccagc gaacttttta cccacgcgta gacaccggct
    23521 tctgagtttt tatttaacga aacaaattct gcgatgatca gacagaaggg aataaaataa
    23581 aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc caagctcaat attattattg
    23641 ataacgttgt taaagctgta aacggcggca aaagtcagcg agagcaagcc gaatttgccg
    23701 attgtattac gtttggtatc agacataaca catctccgaa aatgtgtagg gtagcggtgg
    23761 cggcaaactc cttttgccgc cgtagttgtt atttgttatg gaaataacca tcttcgatgg
    23821 tgactttgag aaccactttt ttgaccgcgt tattgcagat aaaccgatac gcttcatgga
    23881 tatcgcaaat aacgatttgc ccttcgtgga cctctacggt ttctccgcag ccttttaaat
    23941 attcacggtc agtttcatca cgataatatt ccactacctg taatgtctct ttcggcgcat
    24001 attcaatttt ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt cgatgcccgg
    24061 taaatagcgc atcggtcgcg gagtcatttt ccacacggta ggtcaatgaa tcaccgatgg
    24121 agtgggcgac gccaggctga atgttgtcga tattttcaat cgcttcaacg cagcgttgcc
    24181 acttcttgcc agaggcgtaa atctggcgga actgttctaa gttatcgatg atcctcattg
    24241 cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg ccgaactcat acgacgccag
    24301 gctttgcgcg gtagcttctc cgccagaaac cggcagcaac gtaaagccgt agctgaagtc
    24361 acggaaccag acgcgccagg agtccagcac ctcgctgccc caggagttgg agccgaggcc
    24421 aagtagctgg tggtcgaggt tcagggtgat gtcatcactg cgctgcagct cgttacagtg
    24481 ctgggcagcg tggatgtttt cctgggtata gtgccaggcg ctgaagttaa ttgggcgctg
    24541 cggaaccacc agcagaccgt tgccgtggcg gttagtcagt gccgtccagc ggacatgctg
    24601 acggttgccg ttgttctgcg ggaagggata gttctcgaac atggcatcga cggtgctgcg
    24661 ccagatatcg atgatgttag cctgctggct gtcggcgtag ttttcgcccg gtccacgacc
    24721 gtaatacgcc acctgatcgt attcgccgtt aatccccatg gtgaaaccga tgcacggaat
    24781 gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct ggccatcggc
    24841 agcgatgcgc cagatgtagg tacagcgcat cccgaagtca aacaccggcg gtgcaataac
    24901 tgtgcggctg atgatcaaca cttcaccatc gctctgttct acggcaaagt cgcgcagatg
    24961 ttcctgcatg atctgcaaat gattcggctg ccacaaccct tcgtactcct gcttgtggtt
    25021 gtcgatcatc ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt
    25081 tacctggcag gatgtcggtt tgccactcat ttttgagaag gtgatcgcga agttgtagcc
    25141 gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc ggacgcacat tatttggtgc
    25201 gaaaggcact ggctgcgcgg tgttttcctt cagcgggaac tgataagagg cgatagaatg
    25261 cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc
    25321 gcgggcgtcc agctgcggca gcgtgatctg caagggggct tcgctgttcg gcgcaacgtc
    25381 gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag
    25441 ggtgtagtca tcaagcgtgg taaaccacag tttattttcg actttcaact cgccgcgagt
    25501 cagcgcgtgg atttttaccg gcgcgataac ctgtttgtac tctttcaggc ccgatcccgg
    25561 cgtctgatcg gaatagatca aaccatcaag acagaagtta tagttgttgg gatagtcgcc
    25621 gccgaattta taccagacat tgccgtggtc gtcctgtgcc tggatcccgt ggtcgcacca
    25681 ctcccagaca taatgaccct gaatgcaatc gtgcttatag aagacgttct ggtactccgt
    25741 cagcccgccc ggtccgttcc ccatcgcatg agcatattca cagatgatgc gcggcttcgg
    25801 atgcgggtat tcaccaaact cattcatcag cggcacgcgg gtgtacatgg tggaaataat
    25861 atcgaccact tcagcatcgc gatcttcttc gtaatgaacc agtcgcgtgt catccagcgc
    25921 tttcgccgca tggtacatcg cgcggatgtt acagccatag ccggattcat tgcccagcga
    25981 ccagatgatg atcgacggat ggtttttctg tgcgtggata tggcgaacaa tgcgctcgac
    26041 gtagactttt tcccactgcg gatcgtcggt aatacggctg atatcgccga cattagcaaa
    26101 gccgtgcgat tcgacgtcgg tttccgccat cacaaacagg ccgtagatat cacacagttc
    26161 gtaaaaacgc ggatcgttcg ggtagtgagc ggtacgcacg gagttgatat tgtgctgctt
    26221 catcaactgg agatctttct cgacgcgatc cattccaacg gcgcggcctt tgcgatgatc
    26281 gttgtcgtga cggttgacgc cgtgcagcat cacataacgg ttattgatcc agaacagacc
    26341 gtcgcgcact ttgatatcac ggaagccaac gcgttgtggc accacttcca gaacgttgcc
    26401 gttggcgtct ttcagcgtca tgaccagatg gtaaagataa ggggattctg ctgaccattg
    26461 ctgcggctgt tcgacagtaa aagcaaagct ggcgctggtc agtttttcaa ttgccaaatg
    26521 atcaatggcg ctgctgtgca ccacgcgttc gccatcaaac agggtatatt ccagcgtcgt
    26581 gacgacaggg gaggcggcga gattttccag caccacttcg caggaaagcg tggcatcgca
    26641 ataggcttcg tcaaagtcgg tacgcacagt gaaatcgtta atatgcgtta ggtgttttcc
    26701 gaccagataa acatcgcgga agatccccgc cgaccaccac atatcctggt cttccacgta
    26761 ggtagagtcc gcccactgca tcacgcgcac acacaacagg ttgtcgccgg ttttaaccat
    26821 cgcgctgatg tcaaactctg cggtcaggcg actgcccttg ctgaaaccca catactgacc
    26881 gttaacgtag acttcaaaat aggtttcgac gccgtcaaat ttaatcagcg tctgtttacc
    26941 ctgccagccg tcgctgaggg tgaaaatacg ttgataggca ccggttgggt tatcgctggg
    27001 gacaaacggc acatcgatgg ggaacggaaa accttcgtcg gtatattgca gtttgccgtg
    27061 accttccatt tgccacatgg cggggacggt aatatgcccc cagtcagcca ttaactcaga
    27121 ggtgaaggct tctggtactt gcagcggatg gtcaaaaaag tggaaattcc actgactgct
    27181 taagggcaga aacaggctgc tggtttcgcg ggcaaaggta cgcgcttgcg caacagaatc
    27241 atatgaaaaa aagtacgcac gcggcgcaag tcggttttcg tgggtgagct gaatgttttc
    27301 ccagcgattc atagggcctc ccggtagaga gaaatcggaa ccattcatgg tgatggtagt
    27361 ttgtgtaatt gctgggggtt agtaaaattt ttactaaaaa gcagcaggta aatgcattta
    27421 ctttaactgg atcacgaaaa taaacggcag cactgtccgg caaaaatgat tgccggacag
    27481 tgtgtaaggg gatttaacgg gtcgtgccgc gcagttttaa tttgctggga acgaagacta
    27541 gcagcggcag cgcgcgacca tcgcgggctt tttcatacac caggttaacg ccctgacttc
    27601 ccatcatctc ggaatggatg cgcacggtgg agagcggcgg aaaggtaaat cgcgcggtgg
    27661 ggatatcgtt aacgctgata agcgaaatat cttgtgggat gttcaggcca cgttcatgaa
    27721 ttgcccgcag tacgccgata gcaatggaat cggaagcaac aaacagtgcc ttcggatagt
    27781 cttcccgcgc cagcatttgt tttgccagtt cataacccga cgaactggaa aaaccgccgc
    27841 gccagatatc ctcttcgcgt accacttgtt tcagtcggcc atattccgca aaggcgacct
    27901 cacgaatatc cgccttgcca ggctcatctt caccgccaat aaaaccaata cgattaacgc
    27961 cctggttgat atagaagtcg atgatttctt tactgatgcg tgccagatcg atatccaccg
    28021 catcgtaacc gctgccgggt tcgtgaaagt cgataaaaca gatattgtcg gtcaacgcgc
    28081 tggcagcggc gcgcagggcg ggcgtgggtt tgccgacaat taaaataccg gtgacgtttt
    28141 taatgtctgg taatccgttg tgttcataac agttggtcag ttcgatcgcc agcttttcgc
    28201 actgagtttc aatgccgtgg cggatcgcca gatagtaagg atcgttgatc tccagctcct
    28261 gctggtagct gtagatagcc agaatatggt gttggttgac tgcgcctgtc tgtagtttac
    28321 gggcgctact ggtcttgtac tccagctttt cggcgatctc gagaatgcga tgtttcgtct
    28381 cttctttcac attcaatgtc ggatcgtcat ttaagaccct ggatactgtc gccagggata
    28441 cgccagcttc gattgcgatg tcttttaatg ttgccatgtt tacctttttt actttccctg
    28501 atgtgcgccc ccattgtaga ggaagtgctt atcgggtcac ctgttttcag taaataaagc
    28561 gcgtttcttt gacgctgtaa cggtaacaca gcggcttagg aatgtgatca cgcctgcatt
    28621 ctccattttt agcctacagg attgagaaaa acttttctat agtttagtaa aattttatcc
    28681 gaagggagga agcaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    28741 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    28801 ttgatgtggg gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga
    28861 ggagctgatg gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg
    28921 tgaaacgtcg gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt
    28981 gttaacgccg gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc
    29041 gacgtcgtat cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc
    29101 cagcgcctta cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg
    29161 cggttcaatg cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg
    29221 gaacatctcg ctggcaaagt tatagccgat ttcgttatca acaaactcaa tcgccatcaa
    29281 catcccttta ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa
    29341 accgtccagc aacatatcgc ctttttgctc agcctgagcc ggtaagttct gctccagcaa
    29401 cacattgatg gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt
    29461 atgcaggaat gggttgtcga acagaactga aaacacctct tcagtggcga tggtcgcgcc
    29521 aatcggcatc acgccgccgc cgagggcttt tgccagacac agaatgtccg gctgaacgtt
    29581 ctcatgttcg caggcaaaca tcttgcccgt gcgccccatg cccgtttgta cttcatcgag
    29641 gatcatcagt gcgccgaact catcgcacag cttacgcacg gcggtgagat agcccggcgg
    29701 cggcagaatt acgccacctt cgccctgaat cggttcgagg atcaccgcag ccacatcatc
    29761 accggttttt ttgcactcat taagagccgt gcgcatggct tcgatattgc caaacggcac
    29821 atgacggaac cctggcagca gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc
    29881 cgacagcgcg ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa aagtaaattt
    29941 gccgcgcggc gactgataag ccttcgccag cttcagcgcc gcttcaacgg actcggtgcc
    30001 gctattacag aagaagctgt atttcagttt accgggcgtt agcgcagcaa gggttttcgc
    30061 cagcatagcc cgtaacggat cgagtagctc ctggctgtgc agtggttgtt tcgcaagttg
    30121 attctgtacg gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc
    30181 tcccaggcag tcgataaact cctgtccctg ggtgtcgaca agcgtattta aacctcccgc
    30241 ttgccactct acggctccgt aatccccgcc ggcggtaaca gatttgcgat actctaaaaa
    30301 ccccggattg acatgctctt tgaagtattc aatcacctct cggttaagtg ctttcatctc
    30361 ctcatgatcc agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc
    30421 cgatgcgctc gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata
    30481 ccgatttaag tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa
    30541 cacaaatgcg gttatcattt gtgcaatatt taatgctatc cgttaacatt tgttgttatt
    30601 ttgcacggtt tttgtcgggt atggatttga tgattgctgc gcaaatgcac gttttcaggg
    30661 caataattgc ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa
    30721 gtattttaaa tcaaagaatt agatttgtgc tttaatcgca aattgcgatc taaatcaaat
    30781 taatcggtta aagataaccg cagcggggcc gacataaact ctgacaagaa gttaacaacc
    30841 atataacctg cacaggacgc gaacatgtct tctcatccgt atgtcaccca gcaaaatacc
    30901 ccgctggcgg acgataccac tctgatgtcc actaccgatc tgcaaagcta tatcactcat
    30961 gctaatgaca cttttgtgca ggtgagcggc tttaccttgc aagagttaca agggcagccg
    31021 cacaacatgg tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat gtggttcacc
    31081 ctgaaaaaag gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat
    31141 tattgggtgc gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg
    31201 tcgattcgta cccgggcgac ggatgaagag attgcggcgg tggagccgct gtacaaagcg
    31261 ttgaactccg gacgtaccag taagcgtatt cataaaggcc tggtggtgcg taaaggctgg
    31321 ctgggtaaac tgccttcatt accgcttcgc tggcggacgc gtggagtgat gaccctgatg
    31381 tttatcttgc tggcggccat gctttggttt gttgctgccc cggtggtgac gtatttcctc
    31441 tgtgtgttag tggtattgtt ggcaagcgct tgttttgaat ggcagattgt gcgcccgata
    31501 gaaaatgtcg cccgtcaggc actgaaggtg gcgaccggag agcgtaatag tgttgagcac
    31561 ctgaatcgca gcgatgagct ggggctgaca ttacgcgcgg tagggcagct tggcctgatg
    31621 tgccgttggc taattaacga tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag
    31681 acgctggcga aaggcaccga tgaactgaac gaacataccc agcagacagt tgataacgtt
    31741 cagcaaacgg tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg
    31801 gcgtcggctg ccgataaact ttctatcacc gccagtaatg cggcagtgca gggtggggag
    31861 gcgatgacca cggtgatcaa gacaatggac gatatcgccg acagtaccta gcgcattggc
    31921 accattactt cgctgattaa cgatattgcg tttcagacca atattctggc cctgaatgcg
    31981 gcggtggaag cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa
    32041 gtgcgtcatt tagccagccg cagcgctaat gctgccaacg atattcgcaa gctgattgat
    32101 gccagtgctg ataaggtgca atccggttcg cagcaggtac acgccgccgg acggacgatg
    32161 gaagatattg tggcacaggt gaaaaacgtc acccagttga tcgcccagat tagccattca
    32221 acgctggaac aggccgatgg gctttccagc ctgacccgtg cagtggatga gcttaacctg
    32281 atcacccaga aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa
    32341 caccgcgcca gccgactgga agacgcggtg acggtgctgc attaatcggt atgccggatc
    32401 cggcggttgg agcacaatgc ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg
    32461 tgtagcgggt gtaggccgga taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc
    32521 cactggcgga tgcggcgatt aatccagttg cgtaatatca aaagcggcgc ggtcgatcac
    32581 cgcaatgatc ttttttattt gtgcatcact gatatcgctc tggttgacgc gtaaatccag
    32641 caccgcttta aaattatcca gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc
    32701 aacgcaacgc gctttgatgc gttcttcaat catctccact tgttcgcggt tttcttccag
    32761 ccactgcgca ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct cttcgcggat
    32821 ggtaatcagc gactgctcct gcagaaaatc cagcgtcggg tagatgacgc ccgggcttgg
    32881 ggtgtaattc ccctgggtta gattctcaat cgctttaatc aattcgtaac cgtggctgtc
    32941 atcgcgcgag agaatatcca gaatcaccag acgcaattca ccgtgaccaa agaaacgttg
    33001 ccgacgaccg ccgccgcgac cgtggcgacc accgcaacat tgaccgtgtt catgctggtg
    33061 accgtgtccg cagtgctcgt gttctgactt ctcacctttg cagcagccct catgccgtgg
    33121 ctggccttca tgtttacaac acccttcgtg atgatggctc atatcagcct ccttttgctt
    33181 gattagatat atctaattta tatctgatgt gagcattact gcaagtacaa aaatgatttt
    33241 taaacgtgga agcacctcac atctaggccg gataagacgt tcacgccgca tccggcagca
    33301 ttgctatggc actgaatgcc ttcacatact tcagacatat cagttctaca aatcgcttgc
    33361 atttatcatg attaacaatc attatcattt gcgaatttta tttagatata tctgattacc
    33421 atcacgaagg cgataacaat gaataacacc ccccgctacc cgcagcgcgt tcgcaatgat
    33481 ctgcgcttcc gtgaactgac tgtgctacgc gttgagcgca tcagcgccgg ttttcagcgc
    33541 attgtccttg gcggcgaggc gttggacggt tttacatcgc gcggctttga cgatcacagc
    33601 aaactcttct ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagagc
    33661 atcgtctggc aggaaggacc acgcccaccg tcgcgtgact atacgccact gtatgacgaa
    33721 ctacgccatg aactggcgat cgatttcttt attcacgacg gtggggtcgc cagcggctgg
    33781 gcgataaact tacggtggca ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt
    33841 atcagctgta tgtctgcgat gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt
    33901 tgagcaaact tgccgttaaa ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct
    33961 gtcaggatta tctcgcgcac cttgatggtt ttaatatcga atggctggca catgatgagc
    34021 aggcggtaga tgcgcgtctg gcgcagatgc aaatccctgc cgatgattac ttcatctgga
    34081 taaccggcga aggtaaagtc gttaagaatt taagccgccg ctttgaagcg gaacagtatg
    34141 acccacagcg ggtacgtgca gcggcttact ggcacgcaaa gtaactgata tataaaaaga
    34201 aaaaggctga cgatttctcg tcagcctttg ctatatctgg tggcccctgc tggacttgaa
    34261 ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctaaggg gccatggtag
    34321 cggattataa agtaactccg tgtcgcaatc cagccattac cgcgcgcctg ctgtttttat
    34381 aaacaatgta ttttcaatcc gttatacttt tcctggtgat gttagaaagg agtaaatatg
    34441 gttgaggata ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt
    34501 tcatccgccg ggactggttt tccctttgct catccggcaa atcgcttctg gaaggtgata
    34561 tatcaggccg ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat
    34621 tatcgttgtg gcatcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca
    34681 aagcaggagc tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga ttatcagccg
    34741 caggcgttgg cgattctggg caaacaagca tatgaacagg gattcagcta gcgcggtgca
    34801 cagtggggga aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc
    34861 agcggtttaa gtcgcgtttc actggagaaa ctggttgaag cgtatcgcga gctggaccag
    34921 gcgctggtag tgcgtgggcg ataaaa
//
LOCUS       DADLCQ010000031        34634 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000031 DADLCQ010000000
VERSION     DADLCQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.031"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1103)
                     /locus_tag="JQG95_001358"
     CDS             complement(<1..1103)
                     /locus_tag="JQG95_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY5887969.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALV"
     gene            complement(1315..1662)
                     /gene="pspE"
                     /locus_tag="JQG95_001359"
     CDS             complement(1315..1662)
                     /gene="pspE"
                     /locus_tag="JQG95_001359"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY5887970.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1705..1926)
                     /gene="pspD"
                     /locus_tag="JQG95_001360"
     CDS             complement(1705..1926)
                     /gene="pspD"
                     /locus_tag="JQG95_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY5887971.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1935..2294)
                     /gene="pspC"
                     /locus_tag="JQG95_001361"
     CDS             complement(1935..2294)
                     /gene="pspC"
                     /locus_tag="JQG95_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY5887972.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2294..2518)
                     /gene="pspB"
                     /locus_tag="JQG95_001362"
     CDS             complement(2294..2518)
                     /gene="pspB"
                     /locus_tag="JQG95_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY5887973.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2572..3240)
                     /gene="pspA"
                     /locus_tag="JQG95_001363"
     CDS             complement(2572..3240)
                     /gene="pspA"
                     /locus_tag="JQG95_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY5887974.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3407..4384
                     /gene="pspF"
                     /locus_tag="JQG95_001364"
     CDS             3407..4384
                     /gene="pspF"
                     /locus_tag="JQG95_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY5887975.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4504..5769)
                     /gene="puuE"
                     /locus_tag="JQG95_001365"
     CDS             complement(4504..5769)
                     /gene="puuE"
                     /locus_tag="JQG95_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY5887976.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5807..7087)
                     /locus_tag="JQG95_001366"
     CDS             complement(5807..7087)
                     /locus_tag="JQG95_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY5887977.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7089..8576)
                     /gene="puuC"
                     /locus_tag="JQG95_001367"
     CDS             complement(7089..8576)
                     /gene="puuC"
                     /locus_tag="JQG95_001367"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY5887978.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8726..9283)
                     /gene="puuR"
                     /locus_tag="JQG95_001368"
     CDS             complement(8726..9283)
                     /gene="puuR"
                     /locus_tag="JQG95_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY5887979.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9310..10074)
                     /gene="puuD"
                     /locus_tag="JQG95_001369"
     CDS             complement(9310..10074)
                     /gene="puuD"
                     /locus_tag="JQG95_001369"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY5887980.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10286..11704
                     /locus_tag="JQG95_001370"
     CDS             10286..11704
                     /locus_tag="JQG95_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY5887981.1"
                     /translation="METNIVEVENFVQQSEERRGSTFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11853..12017
                     /locus_tag="JQG95_001371"
     CDS             11853..12017
                     /locus_tag="JQG95_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887982.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12007..13392
                     /gene="puuP"
                     /locus_tag="JQG95_001372"
     CDS             12007..13392
                     /gene="puuP"
                     /locus_tag="JQG95_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY5887983.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13526..13771
                     /locus_tag="JQG95_001373"
     CDS             13526..13771
                     /locus_tag="JQG95_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY5887984.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14084..15727
                     /gene="sapA"
                     /locus_tag="JQG95_001374"
     CDS             14084..15727
                     /gene="sapA"
                     /locus_tag="JQG95_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY5887985.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15724..16689
                     /locus_tag="JQG95_001375"
     CDS             15724..16689
                     /locus_tag="JQG95_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial peptide ABC transporter permease
                     SapB"
                     /protein_id="HAY5887986.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16676..17566
                     /gene="sapC"
                     /locus_tag="JQG95_001376"
     CDS             16676..17566
                     /gene="sapC"
                     /locus_tag="JQG95_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY5887987.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17566..18558
                     /gene="sapD"
                     /locus_tag="JQG95_001377"
     CDS             17566..18558
                     /gene="sapD"
                     /locus_tag="JQG95_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY5887988.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKK"
     gene            18560..19366
                     /gene="sapF"
                     /locus_tag="JQG95_001378"
     CDS             18560..19366
                     /gene="sapF"
                     /locus_tag="JQG95_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY5887989.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19434..19787
                     /locus_tag="JQG95_001379"
     CDS             19434..19787
                     /locus_tag="JQG95_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY5887990.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20155..20943
                     /gene="fabI"
                     /locus_tag="JQG95_001380"
     CDS             20155..20943
                     /gene="fabI"
                     /locus_tag="JQG95_001380"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY5887991.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="JQG95_001381"
     CDS             21087..22214
                     /locus_tag="JQG95_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY5887992.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /gene="rnb"
                     /locus_tag="JQG95_001382"
     CDS             22282..24216
                     /gene="rnb"
                     /locus_tag="JQG95_001382"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY5887993.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTTEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="JQG95_001383"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="JQG95_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..24911
                     /locus_tag="JQG95_001384"
     CDS             24450..24911
                     /locus_tag="JQG95_001384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887994.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDALRFSTTKTTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIG"
     gene            24930..25631
                     /locus_tag="JQG95_001385"
                     /pseudo
     CDS             24930..25631
                     /locus_tag="JQG95_001385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            25644..26424
                     /locus_tag="JQG95_001386"
                     /pseudo
     CDS             25644..26424
                     /locus_tag="JQG95_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            26572..26745
                     /locus_tag="JQG95_001387"
     CDS             26572..26745
                     /locus_tag="JQG95_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5887995.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="JQG95_001388"
     CDS             26835..27584
                     /locus_tag="JQG95_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5887996.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27853..28071
                     /gene="osmB"
                     /locus_tag="JQG95_001389"
     CDS             27853..28071
                     /gene="osmB"
                     /locus_tag="JQG95_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY5887997.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28197..28523)
                     /gene="yciH"
                     /locus_tag="JQG95_001390"
     CDS             complement(28197..28523)
                     /gene="yciH"
                     /locus_tag="JQG95_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY5887998.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28523..29260)
                     /gene="pyrF"
                     /locus_tag="JQG95_001391"
     CDS             complement(28523..29260)
                     /gene="pyrF"
                     /locus_tag="JQG95_001391"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY5887999.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29453..30622)
                     /gene="lapB"
                     /locus_tag="JQG95_001392"
     CDS             complement(29453..30622)
                     /gene="lapB"
                     /locus_tag="JQG95_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY5888000.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30629..30937)
                     /gene="lapA"
                     /locus_tag="JQG95_001393"
     CDS             complement(30629..30937)
                     /gene="lapA"
                     /locus_tag="JQG95_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY5888001.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31086..31850)
                     /gene="pgpB"
                     /locus_tag="JQG95_001394"
     CDS             complement(31086..31850)
                     /gene="pgpB"
                     /locus_tag="JQG95_001394"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY5888002.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32020..32610
                     /gene="ribA"
                     /locus_tag="JQG95_001395"
     CDS             32020..32610
                     /gene="ribA"
                     /locus_tag="JQG95_001395"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY5888003.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32674..>34634)
                     /gene="acnA"
                     /locus_tag="JQG95_001396"
     CDS             complement(32674..>34634)
                     /gene="acnA"
                     /locus_tag="JQG95_001396"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY5888004.1"
                     /translation="QPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGK
                     FVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVE
                     KYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNEL
                     EVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTL
                     GLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPI
                     ETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPI
                     GHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTY
                     GWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPA
                     GRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDV
                     VSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSN
                     LIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPC
                     RCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8164 a         9033 c         8745 g         8692 t
ORIGIN      
        1 actaatgcac cttcctgctg taatgcctcg accagcgcta atatttcgct ttctggaagc
       61 aagccacgca gtgggttcaa cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtc
      121 gttttactgg agggcaagct cagactttgc gcccactggc acagcgcctc gacgttctgt
      181 ttttgcaccg catgtaacac cagcggcggc agcgagaact ggtacaccat atgcgcttcg
      241 tcatcaccgt tgccaaagta agcaatgttg tctttgtgcg gaacattggt ttcggtaatg
      301 atcactgtac ctggtgctac gtcatcaata atcgaccgta acagcttgat aatcagatgt
      361 gttttttcca gatggatgca gcttgttccc ggctctttcc acataaagcc aacggcatcc
      421 aggcggacat actcagcacc tttctcaagg taacagagta aaacatccac catcgccagc
      481 aacacttcag ggctacggta attcaggtca atttgatcgt cactaaaggt ggtccataaa
      541 tggcgcgtag aattatcatc catttgaaac ggcgttaata gcggtaacgc acgcggaagg
      601 gtgacggcac tgagatcggt tttcggatca accgcaataa agaaatcttc aaaacccgga
      661 agctgttgta aatagttttt aaaccattca ctttttgcag acatatggtt gcagacaaaa
      721 tcaaacatta aatgactgca ttcaccgagt tgctgaatat cctgccactc ccccgcttca
      781 ctggcgacct gatgataatc aattaccgaa aatccatcat cagatgacca tgggtaaaaa
      841 ggtaataaat gaacatgtga aaaaatgctt tgcagccatt gatggtagaa ctgattaaat
      901 atgggtagtg gttttaaatc attactgtga aattgatcgg cataggtaat taacacgaca
      961 tcactttcat cccagtgttt tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga
     1021 cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc caccgtagat ttcgtccatg
     1081 taatccgtaa ttttctgttt catgttgcct ttccgcggta gcggtcccat gaggcgagag
     1141 actactccgc agaaaataaa ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc
     1201 attttttacc agggaaaaag cgtatttgtg agctgacgca aataatctat agggatgtaa
     1261 ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct catggtgaat tcttttaacc
     1321 tttgaccttc ggcattgcga tgtctttcag gccaccggcg ttctcaacgt gggtatatcc
     1381 catctcgcta aggagctctt ttgcctgccc ggactggcgt ccggcattgc aatacacttt
     1441 cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc tctttcactt ccttcagggg
     1501 aatattgatg gccccctgaa cgtgctcttg ctgatactgc tctggaacac gaacatcgat
     1561 ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt gccagagcaa gtaagccttt
     1621 ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata cgcctgagat
     1681 ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt tattagcagc
     1741 ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac
     1801 tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg taagaagtac
     1861 cagcttgcct gctaatttga aaccaagctt caccttttgc ccggcctgtt gccagcgagt
     1921 attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt atcggaagtg
     1981 acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg gtcgacttca
     2041 tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc cggcattgga
     2101 tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag accgaagaaa
     2161 atcgacagca ccaccaggat acgcaccagt tttaccggta catcaaaata gttggcaatc
     2221 ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataactt tttattgaga
     2281 ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga atagattcca
     2341 gcgcctgaat acgttcgcgc atccgttttg cttcatcaac cagttgcgct aatcgctgct
     2401 gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc cataaccaga
     2461 tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc gcgctcataa
     2521 gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt attattgatt
     2581 gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat catcggcttt
     2641 cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc tttctgcttc
     2701 cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg cttcatccag
     2761 tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa
     2821 catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct cgccaatctc
     2881 tttcttcatg cgtgccagcg tatcgtccac cagcgtcact tcatgttcca gggacttaat
     2941 cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct ctctctcttt
     3001 cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg cttgttcaat
     3061 acggcgagtc agctgtttct tttctgccaa cgcacgcgcc gaagtagaac gtacttcaac
     3121 cagtgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat cttccgcttt
     3181 ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa aaatacccat
     3241 aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta atacaatttg
     3301 cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca acagcaacat
     3361 gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg ccgaatacaa
     3421 agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg tttcgcatct
     3481 cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta aagagttgat
     3541 tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt cccttaactg
     3601 cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag cgggggcgtt
     3661 taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg gtacgctatt
     3721 tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc gtgtgattga
     3781 gtacggtgaa ctggagcgcg ttggcggtag ccagccgttg caggtgaatg tgcggttggt
     3841 atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct
     3901 gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc gcgaaagcga
     3961 cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca agctgcctct
     4021 gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt ggccgggaaa
     4081 tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca ccagcgatta
     4141 tccgcttgat gacatcatta ttgatccctt taaacggcgt ccacctgaag aagccatcgc
     4201 cgtttcagaa aatacctcgc ttccgacgct gccgctggat ttacgcgagt ttcagatgca
     4261 gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc agaagcgcgc
     4321 ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa agcaccagat
     4381 ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat tcggcataca
     4441 taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta ttgcgttggg
     4501 ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt gcgcatccgg
     4561 gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg tcagcaggag
     4621 cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg acggctcgcc
     4681 cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta ccgcagcaat
     4741 ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc ccagttgatt
     4801 cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg cggcagccac
     4861 cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg cgtccataat
     4921 attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt
     4981 cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa
     5041 gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat
     5101 ggcggcaacc agctcttttg gcgcaacgtt gaaaccgcac tcgccctgca ccggttcgaa
     5161 aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt cgatggcgtc
     5221 gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt gatacaccga
     5281 accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca gcgccatggt
     5341 catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc gtccggtatg
     5401 ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga agaatgcggt
     5461 tttagcctgc ccgctcactg gggcaagggt gttaattttc tccgccagcg tgacgtagct
     5521 ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc
     5581 cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg cggcgaaatc
     5641 gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact gggcgaagaa
     5701 gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat ggaattcatt
     5761 gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa acgcccagac
     5821 gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc
     5881 cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgctcg gcgtcaccgc
     5941 gcagcagttc agcaatcaaa cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt
     6001 aaccctgcat gtaatagatg ttggtatcaa ggcggccgaa ctgcggcatg tgcgacaagg
     6061 tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcaac tgagggaagg
     6121 tttttagtag tttcggtacc acaagacgct caacgtcatc cgggttgcgt gcaccataca
     6181 ctacaccccc gccgtacagc aggcggttgt cggcggtaag acggtagtaa tcaagcagat
     6241 agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa tcttccgaca
     6301 gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgct agttccggct
     6361 ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc gtcacctgac
     6421 cattggcagt ccgaaccacg gctggcgtgg tgtgctggat ctgcgtcacg gcggaaagct
     6481 catacacgcg cccgccgttc aggcggatgg catcggcttc accaatggcg aggtttagcg
     6541 ggtgaatatg cccaccgctg tgatccagca gcgcaccagt gtagcgatcg ctggcgacct
     6601 cgcggcgaat agcgttgacg tccagcagct ccagctgttt attgccgtag cgttcccagt
     6661 tctctttctg ctcttcgagt gtggcgagtt gtttatcatt catcgccaca aacagaccgc
     6721 cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg atctcgccac
     6781 cctcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt ttttcgatca
     6841 cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa
     6901 agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg
     6961 cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg attgactcat
     7021 tcagtgtgtc gaatggtgca tatttattcg cactggcggc gtagtaactg ctggtatgtt
     7081 cggtcatttc aggcctccag gcttatccag atggttttca gttcagtgaa tttttcaagg
     7141 gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc
     7201 atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg tcggctcatg
     7261 cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta ctggctgtcg
     7321 ttggcaagct gcagcgcctg atcttctgat gtgaaacggg tgaccaccag caccggaccg
     7381 aaaatctctt cgcggcttaa ggacgcattc gggtccacat ccacgaagat ggtcgggccg
     7441 atggccgctg ccagcccggc gttgcgacca tccagcagca gttgcccctt gccttcgcct
     7501 tcacgaataa aggtgtgtac cgagtcagcg tgagcgctgt cgattaaggt gcccatggtg
     7561 gttgcgggat caagtggatg gcccggctgc cagttttgcg cctgctgttt taacagggct
     7621 aagaattcat cggcgatgct ctcttccagt aacaaacgcg ttccggcgat gcacacctgg
     7681 ccctggttgt agaagatgcc tgcggcggtg gcgcttgccg ctttttgcag atccggacaa
     7741 tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacacg tttcatgttg
     7801 ctatcacccg cgtctttcag cagttgttta cccgtacgcg tggagccggt aaaggcgatg
     7861 gcatcgatat cgttatgacg cgaaagcgcc tgccccgctt catgaccaaa acccgtcacc
     7921 acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca
     7981 ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg
     8041 ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg
     8101 ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca
     8161 tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca
     8221 tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct
     8281 tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga
     8341 gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg
     8401 atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac cggatcaacg
     8461 gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt
     8521 tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg
     8581 actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc
     8641 cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg cgcattgcgc
     8701 ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg ctgataattc
     8761 ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta atggcataac
     8821 tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt tcaccttcca
     8881 gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt gtgcccggct
     8941 ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga accagcttca
     9001 ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg acctgcggct
     9061 catcaggttt ttccggctcg gaaaagaatt ccgagagtga caaaccatac accttcagca
     9121 gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta ctgatagcac
     9181 tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc tgctggcgga
     9241 tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca tttccttaaa
     9301 ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg tgataaagcc
     9361 ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt gccactgtac
     9421 gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat ccggcgagcg
     9481 agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta gagagtttac
     9541 ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt caacctgaac
     9601 ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc gatgttccag
     9661 caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca ccaccagttc
     9721 ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg cattaattat
     9781 cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt tttcaccata
     9841 taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga gtttcggcaa
     9901 aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg gcaagccgcc
     9961 tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat gacccttaag
    10021 cctgttcctg cacattacga caccgataac cggattgttc attatatttt ccatgctcaa
    10081 tctcacaaag tggactaaat tatcgccatt actgcgcttt aaatgacctt tatgttcaat
    10141 attttttcaa tctagcagtg gattttcatt tttgcaaact caatttaaca tttgacaaac
    10201 atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg ataacgagcg
    10261 gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag agaactttgt
    10321 tcagcagtca gaagagaggc ggggtagcac ctttacgcag gaagtgaaac gctacctgga
    10381 gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg gttgcttccg
    10441 tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt atttcccggc
    10501 ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc tgggtcagga
    10561 aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc cttctgccgc
    10621 cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg gcgctccctt
    10681 tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc agcgcggatt
    10741 gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc gcgacgctga
    10801 aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac aaagccaggt
    10861 ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg atgaactggc
    10921 gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg gtcagtttga
    10981 aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc tggcactaaa
    11041 acgcctagtg cgtctgatgg cagaaaagca taagatgcac gccactttta tggcgaagcc
    11101 gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa ataatcgtgg
    11161 cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa agatgctcgc
    11221 cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg tgaactcgta
    11281 tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg
    11341 caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg aatatcacgt
    11401 ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg gtattttgca
    11461 tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc tggaacagga
    11521 aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg agaatgatca
    11581 cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta aaaatgatga
    11641 gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc
    11701 gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg acatttgctg
    11761 gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt
    11821 aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg
    11881 agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct ttgcttccct
    11941 tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc aggggaggca
    12001 gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa aacccgtctg
    12061 cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct cacgccgatg
    12121 accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt tccggcgtcc
    12181 tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa actggttcgc
    12241 cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc
    12301 ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat gatcaacgtt
    12361 ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg
    12421 gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac
    12481 ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg
    12541 gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc
    12601 agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg tttctcgttc
    12661 ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc acgcgtgatc
    12721 ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc ggcgtcgttc
    12781 tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc cgcactgcct
    12841 gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg caccacgttt
    12901 gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct gctgtatgtg
    12961 atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc aaaatggcgg
    13021 actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct gttctttgat
    13081 ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac cttcgtgaat
    13141 ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg gaaggatcac
    13201 ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct gtgggttaac
    13261 ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg cgcatatttg
    13321 tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag aacgccggtg
    13381 agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa cgcgccgcgc
    13441 cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc ccttatgctt
    13501 agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat attttgatat
    13561 tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac gcacaccgct
    13621 ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga tcagcgaaga
    13681 agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg atgagatcgc
    13741 agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat cggtcagtat
    13801 cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca ctgcagcgtg
    13861 ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta
    13921 tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg
    13981 accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca aatgtcatta
    14041 aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc aggtattatc
    14101 gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc
    14161 cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc aagtcaacac
    14221 ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg cccagtttta
    14281 tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgcctgaac ttgccgaaag
    14341 ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt
    14401 tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg tggtgtttac
    14461 cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca gcaacttccc
    14521 ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac tggataatca
    14581 taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc tcgcaaccca
    14641 ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag atcgccagga
    14701 gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg
    14761 gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt taatgccgca
    14821 ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc tgaccgggga
    14881 atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg acgacccgcg
    14941 cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta acaccgccaa
    15001 accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta ataaccagcg
    15061 cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt taccgcgcgc
    15121 ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga aatcgcgcga
    15181 acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc
    15241 gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg atatggcgca
    15301 ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat
    15361 ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg acccggacag
    15421 tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc tcgcccactg
    15481 gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc agctggcagc
    15541 gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac ctattctgcc
    15601 gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc tggtacttag
    15661 cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa
    15721 accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt gttcctgctg
    15781 accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca aggcgcgtca
    15841 ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt tggtgtttcc
    15901 agcattaacg gtcaaccaat tgccgaacag ttaaaagagc tcttcccggc gacgatagaa
    15961 ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg gatgattgcc
    16021 gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt actgggcttt
    16081 tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact cacgctaggt
    16141 tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat taccggtttt
    16201 gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat catgagcgca
    16261 atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc
    16321 cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa agcggcggcg
    16381 acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa cgcgttaccg
    16441 ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc gatgatcacc
    16501 gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat ccgccagcag
    16561 gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat tattgttaac
    16621 gtgatttctg atattttggg tgccatggct aaccctctga aacataagga atggtatgcc
    16681 ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa
    16741 attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac tggctgtact
    16801 gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc tcggttatca
    16861 attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg ggactgacga
    16921 cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg tgggtggcgc
    16981 atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct tcgccggggc
    17041 gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac tggcgatccc
    17101 ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt cccacgctat
    17161 gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca gcatggtgca
    17221 tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat caacgctgaa
    17281 tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg agatcacccg
    17341 cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca
    17401 actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga tttatgtcgc
    17461 cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt tggttaacct
    17521 ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc attactcgat
    17581 attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg
    17641 gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc
    17701 aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg tgttactgct
    17761 gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa
    17821 ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct tgacccttca
    17881 gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa aggccgttgg
    17941 tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg cgtggggatt
    18001 aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag
    18061 aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc tgacgaaccg
    18121 acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac gcgtctcaac
    18181 caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct tagccaatgg
    18241 gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag
    18301 ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat accagacttc
    18361 ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat cccattgctg
    18421 gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc
    18481 attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca tttcccgctg
    18541 aacatggaga aaaagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac
    18601 gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag
    18661 ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt cgggtaaatc
    18721 cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt tattgattga
    18781 cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc gcatgatttt
    18841 tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc tggattttcc
    18901 actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta ttgaaacgat
    18961 gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt tggcacccgg
    19021 acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag tcattattgc
    19081 cgatgaagcc ctcgcttcac tggatatgtc aatgcgttcg cagttgatta atctgatgct
    19141 ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata tcggaatgat
    19201 gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg agcgaggcag
    19261 caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga ttgccgggca
    19321 ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc
    19381 cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat gaggtgatgg
    19441 ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag gaacgaaagc
    19501 tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt caacatccgg
    19561 tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg
    19621 gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg cttgagtcgc
    19681 agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag gaggcggtgc
    19741 tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag ggcgaggggg
    19801 ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga gaatattcgg
    19861 caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat gagcctgttt
    19921 gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg gagagggtta
    19981 gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct ggcaactata
    20041 gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac
    20101 gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt
    20161 tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac
    20221 ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac
    20281 aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag
    20341 tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg gaaagtttgg
    20401 ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt
    20461 gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat cagctcctac
    20521 agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg
    20581 accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca
    20641 aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg
    20701 cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg tatcaaagac
    20761 ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac agttactatt
    20821 gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg tatctccggt
    20881 gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa
    20941 taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaagta atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatgcac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 actgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagctctga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggta aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acctgcgtga tataaccctg cgcgcgagca gatttcacgg aataatttca
    27781 ccaggcttat tcttagctat tatagttata gagagcttac ttccgtgaat cataaattca
    27841 ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg cggctgttct ggcaattact
    27901 ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac gggaccgcaa caccgcaatc
    27961 ggcgcgggtg caggggcatt aggcggtgca gtactgaccg atggcagtac gttgggtaca
    28021 ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg tcggtaaata atcgtaaata
    28081 aatgtcgatt atcgactcgc aattataata cgcttgattc tgttcagtac gaaataactg
    28141 aataatacac acagtaaaat attcgaaagc cacggatata tccgtggctt tttatgttaa
    28201 ccgcctgcga gttttacctt catccctttc gcttcaagta gtgattttaa taaatcacgc
    28261 ttatcgccct ggatttcaat aactccatct ttaactgctc cgccgcagcc gcattttttc
    28321 ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga gatcgatacc ggtaatcagg
    28381 caaacgccct tacctttacg tccactggtc tgacgctgaa tacgcaccgc tccatcgcct
    28441 ttagggcgta ctggggccgc tttgggctca tcaatacgcc cggtttccgt tgagtagacc
    28501 agacggctgt tggaatcact catcatgcac tccgctgtaa agaggcgttg atcgctttca
    28561 gtgtctgcgc tggatctacc gattgcgtta ccgggcgacc aatcaccata taatcaacac
    28621 cagccgccaa cgcctgttct ggcgtcataa tgcggcgctg gtcaccagct tcactcccct
    28681 gcggacgaat gcccggtgta accagtttga actcctgacc gaatacctgt ttaaagcgca
    28741 cagcttcctg agcagaacac accacaccat caaggtcaca tttttgcgtc agtgctgcca
    28801 gacgttctgc ataatctgca ggtgacagtg tcacgccaag atcggccagg tcgctggctt
    28861 ccatgctggt caacactgtc acagcaatca aaagcggtgc atctttgcca aacggaacca
    28921 gtgcctcacg cgctgcggtc atcatacgcg ccccaccgga ggcatgaaca ttcaccatcc
    28981 acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc agtgttgggg atatcgtgga
    29041 atttcaggtc aagaaagata tcaaaaccac gctgttgaag ttcgcgcaca aactgtggcc
    29101 caaacaatgt aaacatctct ttaccgacct tcagacgaca atcgcgtggg tcgatcttgt
    29161 cgacaaagga cagcgcgtca tcacaattat gataatcaag ggcaacaacc acaggagaat
    29221 tcgtaacggc gcgggaagaa gatgaagcag ttaacgtcat gaccagacct tcttgatgat
    29281 gggcaccggg agtggcgcga aacaggtaaa cggcgagcat tctacccgtg aaaataaaaa
    29341 attaacagtt gcgatttttc ccctagcagg ctttttctgc tccgtcgttg ccggagcaca
    29401 atgggacatc aataactagt atgttgtaac taaagtcgga tttttttaaa aattacaggc
    29461 catcaagacc gcgaatcggt ttaatagttg accaggcacg acaagacgga caatgccagt
    29521 agagggtgta tgcagtaaaa ccacatttct ggcagctata acgaggctta ctacgcacct
    29581 tctcgccaac catgtcacgc agcaccatca gactctcttt ggcgcgccct tcttccgctt
    29641 catttaagtg gtaatccatt agcttatgga atacacgcat ggtcggatga cgctgaagct
    29701 ggcgtgtaat atagacctgt gcagcctcac taccgtcgcg cgcttcgatg atatcagcaa
    29761 gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgttgcagg aattctgccc
    29821 attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc gtttcgctga
    29881 ccagttctct gtcctgggat atgacgcgtt gcaaactttc gacggctttg gcgtattctc
    29941 ctttcgccat aaacacgcgt cccatcatga tggatacgcg ggcgctgttt ttatctgccg
    30001 ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc atatgctgca
    30061 gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta cccagcttca
    30121 ccaggcgttc ggcaacatca attgctttct gccactcact ggtagcctgg tagatttgta
    30181 gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga ttgaacatgt
    30241 cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccccagttgt tgaatcgcca
    30301 acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg atgcgaatag
    30361 cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg gcttcaacgg
    30421 tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc tgttgattac
    30481 taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct tcatcttgct
    30541 tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct acaggcaaaa
    30601 gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag tgcggcacta
    30661 cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta cgttcagcgc
    30721 gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa ccgatagcaa
    30781 accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct tgcgctaaca
    30841 gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa atcacgaaga
    30901 tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt atgtggttag
    30961 agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt ttcgcgccgt
    31021 ctgcgaaatg gcatccacgc gcattgttga tgatttaagg cctaaattcg gaaaatcaac
    31081 cagcgttaac tttcttgttc tcgttgtgca atttcgcgat tttcttccgc aggtggtgtt
    31141 aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc ccacgaaatc
    31201 aacgtagcta ccaccagatc gcgtggccaa tgcatcccga gcagcaggcg gcttcccatg
    31261 actcccgttg cccagaccaa caagatagca atggttaacg ttcgccgacg cggccacagc
    31321 aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccgga aggaaaggca
    31381 aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg gaatattttt ctcttcagcc
    31441 aactgttctt ttactagatt tccgcgttct gctcgcttta aagtgtagaa ctcatcaacc
    31501 ggaatatgat gtgttttttc caaccagata acaaaaggtc gtggttcctg tactttgtct
    31561 ttgatccagg atttaacgcc ttgtccaaca aggattgccg ccgccagaat ggcaaataat
    31621 acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa caaaatcaaa
    31681 tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc cgcttttagt
    31741 agccaacttt gttctccagg ttgccaacgc cagccagaaa tccatacggc tactggcatg
    31801 acaagcaata gtgcagctcc cactgcggta cgtcgggcaa tcgaacgcat ggcctctcct
    31861 ttttgataag tcccacaatc ataactgaaa atgccagttc caagaaaaat tgacagattt
    31921 gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc gttatggcaa
    31981 aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa acgtgtggca
    32041 gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga agaactggca
    32101 accggacacg atcatgtcgc gctagtctat ggcgatattt ccgggcatac cccggtactt
    32161 gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg ctgcgattgt
    32221 ggcttccagc tcgaagcggc attgacgcaa attgccgagg aaggccgtgg tattttgctg
    32281 tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc ttacgcactg
    32341 caggatcaag gttacgatac cgtagaggct aaccaccagt taggcttcgc cgctgatgag
    32401 cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga agtccgcttg
    32461 ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa tattgttgaa
    32521 cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga taccaaagcc
    32581 gagaaaatgg gccatttgct gaacaaataa ccctcttgca tcgtgtaatt catttgcttg
    32641 ccggaagcaa aataaccggc aagcaaatac ttgttacttc aacatattac gaatgacata
    32701 atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga tacgacaacg
    32761 gcagggtacg acttcctggc taccatccgc gcgcgtaagc gtcaccggaa ccgtcgcgcc
    32821 aggttgtaga ttttgcagat cgccaatatc aatcttctct tccccggtta gccctaacgt
    32881 tttacgcgtc acgccttgcg gaaattccag cggcaggatg cccatgccaa ttaaattcga
    32941 acggtgaatt cgttcaaacg attcggcaat caccacacga attccaagca gacgaggtcc
    33001 ttttgccgcc cagtcacggc ttgagcctga tccatactct ttcccggcaa tcaccgccag
    33061 cggcgtttgc tcctgcttat agcgcatcgc agcatcataa atagagacta cgtcgctgtc
    33121 aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac ggatgcgaat
    33181 attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac cgtaggagtt
    33241 aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgctgggc tgtcgggctt
    33301 aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca ttgcgaggat
    33361 ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc tgcatttcat caaagaaagg
    33421 cgataagcga atataggttg agtcctcctg ccaaccgtag gtatcggatc gtgtgacgtt
    33481 aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc ggaacatttc
    33541 tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gatggccaga tatctttcag
    33601 ataaaccggc tcgcctttgc gatcatggcc gataggctca gaagccaggt tgatattcat
    33661 atttcccgcc agcgcatagg caaccaccag cggtggcgag gccagccagt tagttttaac
    33721 cagcggatgg atacggcctt caaagttacg gttgccggac agcaccgcac cgacggttaa
    33781 atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt taccaataca
    33841 ggtggtacaa ccgtatccca caaggttaaa ccccagttcg tcgagatacg gtgtcagttt
    33901 cgcttttgcc agataatcag aaacgacttt cgaacccggt gccaacgacg ctttgaccca
    33961 cggttgccgc ttgaggccaa gagttacggc ttttttcgcc agcaagcctg cggccatcag
    34021 cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca cagcgccatc
    34081 aggtaactga tactgatgtc cgttcataac ataatcgact ggcaggcgat ctttatgcgt
    34141 ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg gcagtgcaac
    34201 gcgatcctgt gggcgttttg gccctgccag gctcgcttca acgtcattca tatccagttc
    34261 taacatactg gtaaaaatcg gttcatcgcc cgggttacgc cacatgccct gcgctttggc
    34321 atatttttcg accaactcga cctgatcttc gctgcgcccg cttaaacgca tgtaatcgag
    34381 ggttactgca tcgattggga agaagccaca ggtggcacca tattctggcg acatattggc
    34441 aatggtggcg cgatccgcca acggtagtga atccagacca tcaccataaa attcgacgaa
    34501 tttccccacc acgccatgtt tgcgcagcat ttgggtaaca gtgagaacca ggtctgtggc
    34561 ggtaatacct tcacgtaatt ttccggtaag tttaaagccc actacgtccg ggataagcat
    34621 ggaaaccggc tggc
//
LOCUS       DADLCQ010000032        34087 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000032 DADLCQ010000000
VERSION     DADLCQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.032"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..578
                     /locus_tag="JQG95_001397"
     CDS             <1..578
                     /locus_tag="JQG95_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5888005.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(575..1711)
                     /locus_tag="JQG95_001398"
     CDS             complement(575..1711)
                     /locus_tag="JQG95_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5888006.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1717..2040)
                     /locus_tag="JQG95_001399"
     CDS             complement(1717..2040)
                     /locus_tag="JQG95_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY5888007.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2223..2366
                     /locus_tag="JQG95_001400"
     CDS             2223..2366
                     /locus_tag="JQG95_001400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888008.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(2585..4123)
                     /locus_tag="JQG95_001401"
     CDS             complement(2585..4123)
                     /locus_tag="JQG95_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY5888009.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="JQG95_001402"
     CDS             complement(4288..5439)
                     /gene="yiaY"
                     /locus_tag="JQG95_001402"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY5888010.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(5629..7473)
                     /gene="selB"
                     /locus_tag="JQG95_001403"
     CDS             complement(5629..7473)
                     /gene="selB"
                     /locus_tag="JQG95_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY5888011.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(7470..8861)
                     /gene="selA"
                     /locus_tag="JQG95_001404"
     CDS             complement(7470..8861)
                     /gene="selA"
                     /locus_tag="JQG95_001404"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY5888012.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(8959..9567)
                     /locus_tag="JQG95_001405"
     CDS             complement(8959..9567)
                     /locus_tag="JQG95_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY5888013.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(9638..10723)
                     /locus_tag="JQG95_001406"
     CDS             complement(9638..10723)
                     /locus_tag="JQG95_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5888014.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNSFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(10726..11088)
                     /locus_tag="JQG95_001407"
     CDS             complement(10726..11088)
                     /locus_tag="JQG95_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY5888015.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            11625..13538
                     /locus_tag="JQG95_001408"
     CDS             11625..13538
                     /locus_tag="JQG95_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY5888016.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            13633..14781
                     /gene="mtlD"
                     /locus_tag="JQG95_001409"
     CDS             13633..14781
                     /gene="mtlD"
                     /locus_tag="JQG95_001409"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY5888017.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            14781..15368
                     /gene="mtlR"
                     /locus_tag="JQG95_001410"
     CDS             14781..15368
                     /gene="mtlR"
                     /locus_tag="JQG95_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY5888018.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(15380..15589)
                     /locus_tag="JQG95_001411"
     CDS             complement(15380..15589)
                     /locus_tag="JQG95_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888019.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            15873..16235
                     /locus_tag="JQG95_001412"
     CDS             15873..16235
                     /locus_tag="JQG95_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY5888020.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
     gene            16881..17462
                     /locus_tag="JQG95_001413"
     CDS             16881..17462
                     /locus_tag="JQG95_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY5888021.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            17506..21871
                     /locus_tag="JQG95_001414"
                     /pseudo
     CDS             17506..21871
                     /locus_tag="JQG95_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            22239..23894
                     /gene="lldP"
                     /locus_tag="JQG95_001415"
     CDS             22239..23894
                     /gene="lldP"
                     /locus_tag="JQG95_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY5888022.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            23894..24670
                     /gene="lldR"
                     /locus_tag="JQG95_001416"
     CDS             23894..24670
                     /gene="lldR"
                     /locus_tag="JQG95_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY5888023.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            24667..25857
                     /gene="lldD"
                     /locus_tag="JQG95_001417"
     CDS             24667..25857
                     /gene="lldD"
                     /locus_tag="JQG95_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY5888024.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTILLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            26053..26526
                     /gene="trmL"
                     /locus_tag="JQG95_001418"
     CDS             26053..26526
                     /gene="trmL"
                     /locus_tag="JQG95_001418"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY5888025.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="JQG95_001419"
     CDS             complement(26579..27400)
                     /gene="cysE"
                     /locus_tag="JQG95_001419"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY5888026.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPVRIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="JQG95_001420"
     CDS             complement(27480..28499)
                     /gene="gpsA"
                     /locus_tag="JQG95_001420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY5888027.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(28499..28966)
                     /gene="secB"
                     /locus_tag="JQG95_001421"
     CDS             complement(28499..28966)
                     /gene="secB"
                     /locus_tag="JQG95_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY5888028.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="JQG95_001422"
     CDS             complement(29029..29280)
                     /gene="grxC"
                     /locus_tag="JQG95_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY5888029.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(29422..29853)
                     /locus_tag="JQG95_001423"
     CDS             complement(29422..29853)
                     /locus_tag="JQG95_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY5888030.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            30098..31642
                     /gene="gpmM"
                     /locus_tag="JQG95_001424"
                     /gene_synonym="pgmI"
     CDS             30098..31642
                     /gene="gpmM"
                     /locus_tag="JQG95_001424"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY5888031.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31652..32935
                     /gene="envC"
                     /locus_tag="JQG95_001425"
     CDS             31652..32935
                     /gene="envC"
                     /locus_tag="JQG95_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY5888032.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            32939..33898
                     /locus_tag="JQG95_001426"
     CDS             32939..33898
                     /locus_tag="JQG95_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY5888033.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(33885..34087)
                     /locus_tag="JQG95_001427"
                     /pseudo
     CDS             complement(33885..34087)
                     /locus_tag="JQG95_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         8469 a         8932 c         8691 g         7995 t
ORIGIN      
        1 ttgagcgtaa tgccttttat gaaggcgtcg aatttcctga tgattgtgat gttttaatca
       61 gctacgctcc accaaaagat gaatcgctgg ttgccagttt tattacacaa tatgccgtaa
      121 ccgcttatgc cagccagcgc tatcttgaga aacatcctat tagccgtcct gacgaactcg
      181 aacatcactc ctgtattttg atcgactcga tgatgatcga cgacgcgaat atctggcgct
      241 tcaacgtcgc cgggagtaaa gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg
      301 ataacacgca atcggcgctg gagctggcac gaaatcacct ggggattgtg tttgcgccag
      361 acaaaagtgt gcagagcgat ctgcaagacg gcacgctggt gccctgcttt cagcacccgt
      421 atgaatggtg gctggatctg gtggctatct tccgtaaacg ggaatacctg ccctggcggg
      481 tccagtatgt gctggatgaa atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc
      541 aactgcggcc tgaacaagcg gcggaaagcg aagattaatg atcgagataa aggtaatgca
      601 cccagctggt catgcgtaat agcactttgc gcatcaccga gacatggctg aaatgatcag
      661 agtagaccgc cacctgggcg taaataccgt ctggtaaagc gctcaaatca gtgtgctcat
      721 ccagttcaat ggtcgcgata acgccatctg aacccggcgc tgtgtttaac gtctgtaagg
      781 tgccggtcga ctgataagct ccgccgggaa cagcaggact aatggctgcc agcttgccgc
      841 tgaatacctt acctggcaga gcattaaaca ccacttccgc atcgtcgcca ggggccagtc
      901 gcagcaagga gttctgacgg aactgcgcca caatttgtcg tttctgatcg ggtataaaca
      961 ccatcaccgg acgtagcggc agcgacgcgg cataggtacc cggacgaatc agcacctggg
     1021 tcacataacc atcgctcggc gcacgcacta tcgtctgctc aaggttatat tttgcttccg
     1081 cgagctgcgc tttcaggctg gcgatttgag aatgttcacc caacaccagg ctatccagct
     1141 ggctctggat ctgtttttgt tccgccgccg atgactttac ggaggcttcc tgcgccagat
     1201 aattttgccg cgccacatca atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc
     1261 cgcgtgcgta acgctgatac tctttagcga atttatcccg cgtggccttt gcctgctgag
     1321 tattcgccgc catctcatct aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa
     1381 cgatatccgc catcagccga tccacccgcg cctgataacg cgtcgggtcc agtcgaaata
     1441 gcacctcacc ttttttaatc agcgtatttt tcttatccgt cacttcgatc accacgcctg
     1501 tcacctgtgg gacaacagga atagaaataa ccgctttttg cgccttgaag gtatacggat
     1561 ggttatagtt cattaacaga attaaaccac tgacaataaa tatgccaccc agggccgctg
     1621 tggaaatggt ccatttattt acaggaattt tgaagatctt aaacattgcc catgcaaaag
     1681 ccacataggt caaaataatc agtaaatcca taagtattac tcggcagaag aggatttgat
     1741 gtcggcgagc tgtttctcca gcccggcaat acgctgttgt agttgcacaa cggaggagtc
     1801 atggatttgc attccccatc cccgctccgg gcggtaaagc gtggcccaaa tccacaaaaa
     1861 tggccagata acatgcagcg taaaaagact cacccaacca gcaacatgaa tggcgtcggc
     1921 atgaggatga ttacgttttt tagcaatcag gtagggaata tcatgtaaaa taataatccc
     1981 atagaatatc accagaaata caaaaataag cactcccagt gcaaaatagt ccaggaacat
     2041 atttccctca aagaatataa aaaagaacaa ttaacgcata ttatgaaatg ccatgatgca
     2101 gtgcaatttc cttatttata taacgacata ttgtttttta tatactttct gaaatgtgct
     2161 gtctggtttt tcaacaggac gaggtttatc atattgaaat agtggaacta ttggccaaac
     2221 taatgaataa ctccagtaaa acatccacag tacagattaa gcgtattaaa ccttcaatta
     2281 tctaccgttt attgctgatt ggcctcggat caccaatggt gatttacggc ctggttcgcc
     2341 cgctcaccat cgaaacgcga gattaaactc ctgacaaaac acgccccaga aacgcacttc
     2401 tgttggcgta gtctgggtta ttgcgcacgt aggtttaaaa cgtaaattgt aacaacgtgc
     2461 gctttgttta tgccggatgc ggcgtgtacg ccttatccgg cctacggtca ggttcccgta
     2521 ggcatgataa gacgcgtaag cgtcgcatca ggcaatgaat acccaatgcg accagcttct
     2581 tatatcagaa cagccccaac ggtttatccg agtagctcac cagcaggcac ttggtttgct
     2641 ggtaatgctc cagcatcatc ttgtgggttt cgcgaccgat acctgattgt ttgtagccac
     2701 caaacgcagc atgtgccggg taagcgtgat aacagttggt ccacacgcgc ccagcctgta
     2761 tgccgcgccc catcttatag gccagattac cgttgcggct ccagacgccc gcgcccaggc
     2821 catattgcgt atcgttcgcc agctccagcg cttcttccat cgttttgaag gtggtcaccg
     2881 ccagcaccgg gccaaaaatc tcctcctgga acacccgcat attgttctga ccaaacagaa
     2941 tcgtcggttc gaggtagtag ccgtctttca gttcaccttc cagcagcttg cgccgcccgc
     3001 ctgtgagcac gtcagcgccc tcttttttac cgatatcaat gtagttgagg atggtttcca
     3061 gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct gtcgagcggg ttaccgctgc
     3121 gaatgctttc gacacggcgg atggcgcgtt ccataaagcg ttcgtagatt gactcctgca
     3181 ctaaggcacg actcggacag gtgcaaactt cgccctggtt aaaggcaaac agtgcaaagc
     3241 cttccagcgc tttatcgaaa aaggcatctt cttcatccat cacatcagca aagaagatat
     3301 ttggcgactt accgcccaac tccagcgtca ccggaataat gttttgcgtt gcgtattgca
     3361 taatttgttg gcccacttcc gttgagccgg taaacgccac tttggcgatg cgtttcgagg
     3421 tcgccagata ttcgccaatt tccccacctg cgccattgac cacgttcacc acgcccggcg
     3481 gcagtaaatc accgacaatt tccattagca gcagtacaga aagcggggta agacgtgcgg
     3541 gtttcagcac cacacagttg cccgccgcca gcgcgggagc cattttccag ctcgccatca
     3601 gcagcgggaa gttccacggg ataatctgcc ccaccacgcc taacggttca tggaaatgat
     3661 aggccacggt ttcgctatca acttcactga tcccaccttc ctgcgcccga atacacgagg
     3721 cgaaatagcg gaaatggtca atcgccagcg gtacatccgc agcactggtt tcgcgaatgg
     3781 gtttgccgtt atcccaggtt tcagctgtcg ctaacagctc gaggttttgt tccattcgat
     3841 cggcaatctt aaacagaatc gccgcgcgat cctgcaccga ggtgtgcgcc catttatctt
     3901 tcactttgtg cgcagcatcc agcgccagat cgatgtctcg tttgcccgaa gacgccactt
     3961 cgcacagcag ctgcccggtc accggcgtca gattctggta atactcacca tcggcagggg
     4021 ctacccattc accgccaata aagttgtcgt agcgggcttt taacttgatg gggaaaccat
     4081 actcgccggg cttaatctgt gctgaagggg gattattggt catgatcgtc tccttgcggt
     4141 gtgaggtata acaagggtag acgtgactgg cgaaatcttc gccagtcggt aacagcttta
     4201 cgacagctat cacgaattta cgggcaagga ttacagagat agttgaaaaa gcggctgaca
     4261 atttgccagc cgttgttgga atgatgatta cattgcggcg cgataaatca ccacaatttc
     4321 ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca tctttcaggg cattagtcgc
     4381 cagaaccgcg aaatcttctt ctttcacgtt caggtcgcgt aggcctgccg ggatatccac
     4441 tttcttcgcc agttcacgga tggcgttaat gcaggcttca gcaccttccg cgtcgttttt
     4501 acctgtcacg ttcacgccca ttgcagcggc acagtcacgc agacgtgcag cggcgacttt
     4561 gctgttgaat acctgaacat gcggcagcag aacggcgtta catacaccgt gtggcaggtt
     4621 gtagaaaccg cccagctggt gcgccatcgc atgaacataa cccagagaag cattattgaa
     4681 cgccataccg gcaaggaact gggcataagc cattgcttca cgcgctttcg cattactgcc
     4741 atcttcaacg gctaacggca ggttttcggc aatcatggtc acggctttca gtgcacaagc
     4801 gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg atagcgtgcg ttaaggcatc
     4861 catacccgtt gcggcggtca gtgacttcgg cataccaatc atcagagagg agtcattgac
     4921 agaaagcagc ggagtcacgt gcttatcaac aatcgccatt ttgttatgac gcgcttcgtc
     4981 agtgatgatg cagaaacggg tcatttcaga ggccgtaccc gccgtggtat tgatggcgat
     5041 catcggcagc tgcggttttg cagagcggtc aacgccttcg taatcgcgaa tatcgccgcc
     5101 attggctgcc accagcgcaa taccttttgc gcagtcgtgt ggagaaccac cgcctaagga
     5161 gatcacgcta tcgcaattat tttctttaag taatttcaaa cctgcggcga cgttttccgt
     5221 ggtggggtta ggttgggtgc catcataaat aacgctaaaa atattgcgtt cttccagtgc
     5281 tttttgcaca tcacccgcca tacctaattt cgttaacata ttgtcagtga caattaaggt
     5341 acgggtaaat ccataatctg ccatcatatt cattgcatca gtcaatgaat cagcgccgat
     5401 gacattcaca gaaggaataa agaaagttga agatgccata acacactcct gaaagtgtaa
     5461 aaaagggtgg gcagcttact gaggattttc attcgaaaac atgatcatta ttattaatta
     5521 tcaaaattaa tctaataaaa agcacattgt attcattaaa caatgtgctt tatttaaaat
     5581 cataagaaat ttgtattaat ttattttaaa aattaattaa tcatttcctt atttttccgg
     5641 aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa agccaatgcg
     5701 gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc gatcgcggaa
     5761 atccgccgcg caggttgaac cacactcctg atcgagatcg cggatcatat tggcaaattc
     5821 gacaatccga tcgttacggt aataacgatc tttgacgatc gcggtaatta ttccttgctg
     5881 cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gtacccgtct cttttgccag
     5941 atcacgcacc caccacggct cgtcaccaaa cagcggctct gctttttgcc agatggcctg
     6001 ctgctcttcg ctgaagcccg ctttgtgatc cggcagatgc agccaaccgt gatgactgag
     6061 gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt actagcgctt catcttccat
     6121 tggcaacgcc atacgtcgca gacgttcgcg cccaggccca ggttcatcgc gatgttgctc
     6181 atgataagtc gctaatgtgt cgagaatttt ccgttgccag cgagcggcaa ccggcgcatt
     6241 caacaagcta taaccagcct gaatatagcc aggctgttgc agcagttcgc gcatcccttc
     6301 gccgttgagc tggcgcgccc aggcgaaatc aggaagatta accgcgccgc gttgcaggtg
     6361 aacggctaac gcttcgctat cattctgtgc cctggcgagg gaagccagcc attgcagata
     6421 ctctggttta cgtttaccgc gacgcggcgg gttaagcatc acgacgcgcg ctccggccag
     6481 cgtgttgcgg gcagagatat cgcgcaatac caggcggtcg ttatctgcca gccataacgg
     6541 ggtgtcgaag accagttcag caaggttatc ttccagcagt gaaacgcgtc ccgtgacgtg
     6601 gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgcat gggtttgaag
     6661 ctcgacaatc acccgtgtga acggctctgg cggcacatcg gcaagcagcc agtcgccacg
     6721 gttaatctgc tctttttccg catcacccgc gatgtcaagc gcgatacgct gcccggcatt
     6781 ggcggtttct gttggctggt tttgcgcatg cagcgcacgt acacgcatcg gtttatttac
     6841 accagtcagc cagagtgaat cgcctacctt cacttccccg cttaacgccg taccggtgac
     6901 gaccagcccg gcacctttta cggtaaatgc gcggtcaatc gcgaggcgga aactatgttg
     6961 gctggcgtgc tcgcgttccg gcaactgaag cagatgctcg cgcagggcat caattccccg
     7021 accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt cccgcagaac
     7081 ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat cggctttggt
     7141 cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat gctcacgggt
     7201 ctgtgccatc acgccgtcat cgcacgccac caccaacagc gcgtgatcga taccaccaac
     7261 gcccgccagc atgttggaaa gaaacttttc atgaccggga acgtcgataa aaccaggcac
     7321 gcgaccatcc ggctgcggcc agtaggcata gccgagatcg atggtcatgc cgcgcttttt
     7381 ttcttccggc agacggtcag catttacgcc agtaatcgcc tgcaataagg ttgttttgcc
     7441 gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa aaaccgttgc
     7501 tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg accaatcacc
     7561 ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc gcgtccatca
     7621 tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagcga accactgcca
     7681 atctgcgaaa gacatggcat aacctgtacc gcaaactccg cgccgtaatg tgcgacaagg
     7741 ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt aagcaggcgc
     7801 agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa cgtggcttcc
     7861 agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg gctttgcagg
     7921 cgggcgatca tctctttttt accaacgata atcccggcct gcggcccgcc taacaacttg
     7981 tcgccggaga agctcaccag actgacgccc gccgcaatca actcctgcgg cattggctct
     8041 ttcggcaaac cgtactggct aagatcgacc agcgagccac tgcctaaatc agtcaccact
     8101 ggaacatcca gctctttgcc gagcgccacc agttcagctt cgtctatcgc tttggtgaac
     8161 ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gtgcggtatt ttcattcacc
     8221 gcctgacgat aatcattcgc gtgcgtgcgg ttggtggtcc ctacttcgtg tagggtgcag
     8281 cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac cagttcgccg
     8341 cgagatacca ccacctcttt cccgctggca gtggccgcca acatcaataa caccgccgcc
     8401 gcattgttat tgacgataca ggcatcttcc gcccccgtaa tacggcacag cagctgcgcc
     8461 agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag ggtcactggc
     8521 gaacgcatag cctgcgcaac ggcttccacc gcggcttccg cctgtaaagc tcgcccaagg
     8581 ttggtatgca gcacggttcc cgtcaggttg atcaccggac gcagcgcgct ctgcgcctct
     8641 ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcagg cagcgtctgg
     8701 ctgccacgaa tcacttctcg cgcttcgtcg agcatctgac gcaacaattc caccacgcgg
     8761 gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa gcgatcaata
     8821 gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct ggctgatcaa
     8881 ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt gagatgggtc
     8941 aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac tttcgcggct
     9001 aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccaac ctggcgcaac
     9061 gcggcggaaa ttgagatatc cgacagcaca ggcaatggcg atagtcgcca gattgaccgt
     9121 atcggttttg agtgtgccat cgaccagata tccttccagc acatccagac tgcggttgat
     9181 tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac gcgcctgttc
     9241 acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa ttttgcgcac
     9301 ccgcaacgac tccagcggat cgcgcggcaa catcgccgga gcgacattca ttaactcaat
     9361 atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca ccagcaccgg
     9421 cactttccct aacgggttaa attgcgccac gccgttatcc gcgttatagg gcagttcatt
     9481 aataaattcg aaagttatgc ccttttctaa caacagaata gaaagtttgc gtacaaacgg
     9541 gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa aatatcagta
     9601 tggctcaggc tcacgcaaaa cacaggtgat ataagtctca atgatccaaa taaagataat
     9661 gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg ctgaaatggt
     9721 cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag
     9781 ggtccagttc aatggttccc agcacaccgt ccgtgccggg aacgaccgtt aatgattgca
     9841 ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatacta gtcaatttgc
     9901 cgtggaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg ccaggtttca
     9961 gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc tcggggatga
    10021 agaccatcac cggacgcagc ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct
    10081 gagtgacgta gccattgctt ggtgcgcgaa tgacagtctg ctcaagatta tattttgctt
    10141 cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttgacc atactgtcga
    10201 gctgactctg gatctgcgcc tgctccgcca ccgagccttt cactaacgca tcctgcgcga
    10261 ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cgaattcacc gccgcctggc
    10321 tgcctttcag gtaacgttga taatttttaa acagacggtc gcgctccgct gaaacctggg
    10381 tggtgttggc ctgcgcttca gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg
    10441 tcatcaggtc agcctgaagt ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa
    10501 aaagcacctc gcccttttga ataagctgat tattcttgtc agtgacttca gtaacaattc
    10561 ccgtcacctg tggcgtgata gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag
    10621 ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct cccagcgccg
    10681 ccgtcgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa
    10741 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    10801 agaaagtgcg gcgatttccg gttcagtcgc tttctcctgc gacgcaacat gtgactgcat
    10861 accccagcca cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac
    10921 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt
    10981 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    11041 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    11101 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    11161 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat
    11221 gagttgctta gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca
    11281 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa
    11341 aacaaaaatg tgacattact cacatttaaa tgccattttt agcgaaaatc accgccttgt
    11401 ttctttttta tacaggcgtt ttgtgatgag cgtcacgtca attacctctc taccccctat
    11461 atttatgtga ctgatatcac acaaaagccc gtcgactgga cagttaaccg attcagtgcc
    11521 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    11581 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    11641 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    11701 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    11761 agacgctggc gaagctggtc gggccgatga tcacttatct cctaccgctg ctgatcggtt
    11821 ataccggtgg taagctggta ggcggcaaac gtggtggcgt agtcggtgcc attaccacca
    11881 tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc
    11941 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    12001 ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    12061 cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta
    12121 acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa
    12181 tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt
    12241 cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    12301 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    12361 cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga
    12421 atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc acgctgacta
    12481 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    12541 tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct
    12601 ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    12661 aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca tctccgctat
    12721 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg
    12781 ccggtatggg ttcaagtgcg atgggcgcag gcgtgctgcg taagaaaatt caggatgcag
    12841 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    12901 tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    12961 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg
    13021 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    13081 ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    13141 aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    13201 acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc
    13261 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    13321 cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    13381 ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca
    13441 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    13501 tggatgaagt gctggaactg ctggcaggtc gtaagtaatc caatcctacc ctctcctcac
    13561 ggagagggtt tgggtgaggg aaaagcctca ccccagccct ctcgggtaaa aacattgatg
    13621 aaggttaata ctatgaaagc attacatttt ggcgcaggta atatcggtcg tggctttatc
    13681 ggtaaactgc tggcagacgc gggtatccaa ctgacgtttg ccgatgtcaa tcaggtggta
    13741 cttgatgccc tgaatgcccg tcatagctat caggttcatg tggtcggtga aaccgagcag
    13801 gtagataccg tttccggcgt caatgctgtc agcagcattg gtgatgatgt cgttgatctg
    13861 attgctcagg tcgatttagt cactaccgcc gttggcccgg ttgtgctgga acgtattgct
    13921 ccggctatcg ccaaagggct ggtgaaacgc aaagaacaag gtaatgaatc cccgctgaac
    13981 atcatcgcct gtgaaaacat ggtacgcggc accacgcagc tgaaaggtca tgtgatgaac
    14041 gccctgccgg aagacgccaa agcgtgggta gaagagcacg ttggctttgt cgattccgcc
    14101 gttgaccgca tcgtaccgcc ttcggcttcg gcaactaacg atccgctgga agtgacggta
    14161 gaaactttca gcgaatggat tgtcgataaa acgcagttca aaggcgcact gccgaacatc
    14221 ccaggcatgg agttaaccga caacctgatg gcatttgtcg aacgtaaact cttcaccctg
    14281 aacacgggtc atgctataac cgcgtacctc ggaaaactgg ccggtcatca gaccattcgt
    14341 gacgctattc tcgacgagaa aatccgcgcg gtggtaaaag gtgcgatgga agaaagtggt
    14401 gcggtactga tcaagcgcta cggctttgac gcagacaaac atgcggcgta catccagaaa
    14461 atcctcggtc gttttgagaa cccgtatctg aaagatgatg tagagcgcgt aggccgtcag
    14521 ccgctgcgta aactgagtgc tggcgaccgt ctgatcaagc cactgctcgg tacgctggaa
    14581 tacagccttc cgcacaagaa tctgattcag gggattgctg gtgcaatgca cttccgcagt
    14641 gaagatgatc cgcaggctca ggaactggca gcactgatcg ctgacaaagg tccgcaggcg
    14701 gcgctggcac agatttccgg tcttgatgcc aacagcgagg ttgtatccga ggcggtaacc
    14761 gcttataaag caatgcaata atggtggacc aggcgcagga caccctgcgc ccgaataaca
    14821 gattgtcaga tatgcaggca acaatggaac aaacccaggc cgttgaaaac cgtgtgcttg
    14881 agcgtctgaa tgctggcaaa accgtgcgaa gctttctgat caccgccgtc gagcttttga
    14941 ccgaggcggt aaatcttctg gtgcttcagg tattccgcaa agacgattac gcggtgaagt
    15001 atgctgtaga accgttactc gacggcgacg gtccgctggg cgatctttct gtgcgtttaa
    15061 aactcattta cgggttgggc gtcattaacc gccaggaata cgaagatgcg gaactgctga
    15121 tggcattgcg tgaagagcta aatcacgacg gcaacgagta cgcctttacc gacgacgaaa
    15181 tccttggacc ctttggtgaa ctgcactgcg tggcggcgtt accaccgccg ccacagtttg
    15241 aaccagcaga ctccagtttg tatgcaatgc aaattcagcg ctatcaacag gctgtgcgat
    15301 caacaatggt cctttcactg actgagctga tttccaaaat cagcttaaaa aaagcctttc
    15361 aaaagtaagc aacgtctgct tactgcccct ctacctgctt cggccgataa agccgacgat
    15421 aatactccag acgttgtaga tataacggca cgctttcatc gggtattccg gacggaatcg
    15481 cgttacgggg aggaagtttt ttcagatact cccggaacgc ctggcttgat gccatgaaat
    15541 ctacggcttt atcgataaga agcggaacgt tttcacctaa tttgcccatg atgatatctc
    15601 cgtattaccc cgcgccggga atgcgcggcc tccaatttta gtttaggttc ccggtaaaga
    15661 tgacattaag gaaaacgtgc tgaatcctca ataatcatcc gctgctgttt attatctttt
    15721 tctatagatt tcttaattaa tcaacgaatt gcgtagtttt tgagcatgtt aatgattacg
    15781 ttgcagaact attacaatgt catcagttgt gaacaaagca cctggtcgcg catactaggg
    15841 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
    15901 agcgtctcag cgatcgcctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
    15961 aagccgcaga caaatatgcc gagctggaaa aagagaaagc cacgctggaa gcagaaattg
    16021 ctcgcctgcg tgaggttcat agccaaaaac tgagtaaaga agcacagaag ctgatgaaga
    16081 tgccgttcca gcgcgcaatt accaaaaaag agcaggctga tatgggcaag ctgaagaaaa
    16141 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct ggggcgcgaa atgggcctgg
    16201 aagagatgac tggattttct aagactacgt tttaagcccc tcagacgact ggttcgatcg
    16261 tatcaacccg catctggctt gccttagctg gtcaatgcgg ggactctcct gtctactcgc
    16321 ctaattacca aacaattcac tccctttact tcgatttacc gctggttgat attctgaaaa
    16381 tgcattttta ccgaaatagc catctataaa caataaaaat atatagaaga tatcatttgt
    16441 taattaacaa gcatttacgt ctcttttttt cggatttaat tgtgaaattt taatcatcat
    16501 attcacaaaa tcaattatct gtgaaatttt tcccagacat ggtttctttc atatttgaca
    16561 tttattaata accatcatta gaatccacga aaaataatat attcatacaa acacagacat
    16621 cttttgaaac gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca
    16681 catcactgtc attaatgtaa agcaacatag ctcaccaata atacaatgga caactctacc
    16741 ggcattgatc gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt
    16801 tcagtgctgt cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag
    16861 gcaagccaga aagtagttta ctgaaacaac aagaagaaca taataataaa attgtgctac
    16921 ttgaaaaaca acaagcacag ctaaagagtc aactcgaaac tattcaaaaa caacaaactg
    16981 gcataataaa cagtacaaag actttaactc acgtaattaa atccgttaaa gaccaacaaa
    17041 atacttttat ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaacg
    17101 gttcggtggc tttagcgggt cgagtgttat ccattgacgc cacagagaat ggtagtgtta
    17161 ttcatatctc actggtcaac ttattaagta cacctatctc aaacattggt tttaatgcga
    17221 catggggtgg tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt
    17281 tcaacacatc aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt
    17341 tgaccctgaa gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg
    17401 aaaatattca gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat
    17461 aaatgcagtt agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt
    17521 aaagttatct ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc
    17581 cgtggcaaga aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg
    17641 ttgtcgtcgt ttggggcatt ggcgaatgcc gggaatgaca acggtcaggg tgttgattac
    17701 ggtagtggat cagctggcga cggctgggtt gctataggca aaggggcgaa agcaaatact
    17761 tttatgaaca ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa
    17821 tatagctctg ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg
    17881 gttagtgcaa tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg
    17941 ggccaatact caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct
    18001 atgggggact cttccaaagc ggaaggagca aacgccattg ccctggggcg tagcagtgta
    18061 gctagcggta ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct
    18121 attgcgatag gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg
    18181 aaggcaaaag ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat
    18241 actatcgcat taggaaatag cagccaggct ctggcagata atgcaatcgc cataggccag
    18301 ggcaacaaag ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc
    18361 ttaaacacca ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt
    18421 ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg
    18481 gatttagaca ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg
    18541 aaaaatggcg cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc
    18601 attagcgact cggtagcaaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg
    18661 tactgttaca gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc
    18721 gctcgctgta cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc
    18781 tgctcatggt actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat
    18841 ttcagcctcc agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt
    18901 cgaagccaac accaccaata tcaccaacct gacggattcc gttggtgacc ttcaggctga
    18961 tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc aggataccac
    19021 cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg atgctgttaa
    19081 cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca atatcgccaa
    19141 taacacttcc aatattgcca ctaacaccac caacatctct aacctgactg agacggtgac
    19201 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca cggcagctca
    19261 tggcaccgag accaccagca aaataaccaa cgttaaagat ggcgacctga cgactggcag
    19321 caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg cgacgaatac
    19381 caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg tgactaatct
    19441 tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag ctcatggcaa
    19501 caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa ccagttccga
    19561 tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct acttcggcgg
    19621 caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa tcggtgaaac
    19681 aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta gcacgtctct
    19741 cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac acggtactaa
    19801 tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact ccagttctga
    19861 cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg cgctgggggg
    19921 tggtgccgaa gtcaatgcag acggcaccat caccgcaccg acttacacca ttgcgaacgc
    19981 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
    20041 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
    20101 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
    20161 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
    20221 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
    20281 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
    20341 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
    20401 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
    20461 aggttctcgt gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga
    20521 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
    20581 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
    20641 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
    20701 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
    20761 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
    20821 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
    20881 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
    20941 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
    21001 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
    21061 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
    21121 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
    21181 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
    21241 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
    21301 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
    21361 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
    21421 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
    21481 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
    21541 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
    21601 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
    21661 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
    21721 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
    21781 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
    21841 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
    21901 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
    21961 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
    22021 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
    22081 aactcacatc aatattgcca atacataaca tttagttaac cattcattgt cattatccct
    22141 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
    22201 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
    22261 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
    22321 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
    22381 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
    22441 cagtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
    22501 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
    22561 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
    22621 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
    22681 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
    22741 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
    22801 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
    22861 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
    22921 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
    22981 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
    23041 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatatga
    23101 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
    23161 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
    23221 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
    23281 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
    23341 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
    23401 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
    23461 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
    23521 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
    23581 tcctcggctg gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
    23641 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
    23701 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
    23761 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
    23821 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
    23881 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
    23941 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
    24001 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
    24061 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
    24121 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
    24181 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
    24241 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
    24301 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
    24361 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
    24421 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
    24481 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
    24541 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
    24601 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
    24661 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
    24721 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
    24781 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
    24841 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
    24901 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
    24961 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
    25021 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
    25081 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
    25141 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
    25201 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
    25261 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
    25321 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
    25381 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
    25441 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
    25501 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
    25561 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
    25621 attgcgctcg gtgccgacac catactgctg ggtcgtgctt tcctgtatgc gctggcaaca
    25681 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
    25741 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
    25801 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
    25861 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
    25921 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
    25981 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
    26041 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
    26101 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
    26161 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
    26221 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
    26281 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
    26341 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
    26401 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
    26461 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
    26521 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
    26581 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
    26641 ttgatggctt atcgctgtct ggtttaccga caatacgaac cggaacgcca gcggcggttg
    26701 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
    26761 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
    26821 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
    26881 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
    26941 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
    27001 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
    27061 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
    27121 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
    27181 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
    27241 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    27301 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    27361 taatattgtt ccagacaatt tccagctctt cacacgacat tgcttactcc acacgatgag
    27421 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    27481 tagtgactgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    27541 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    27601 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    27661 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatacc aaaacggcgg
    27721 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    27781 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    27841 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    27901 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    27961 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    28021 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    28081 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    28141 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    28201 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    28261 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    28321 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    28381 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    28441 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    28501 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    28561 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    28621 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    28681 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    28741 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    28801 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    28861 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    28921 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    28981 taagtgttgt ccttaaatac tgcgcaatgt aataccaacg ttcacacgtt atttcagcag
    29041 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    29101 tgcgtcaata aaaatctggg gaaccgtggt gcaaccgctg cgtttgatca tctcttcacg
    29161 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    29221 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    29281 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    29341 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    29401 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    29461 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    29521 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    29581 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    29641 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    29701 tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    29761 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    29821 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga
    29881 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    29941 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    30001 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    30061 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    30121 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    30181 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    30241 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    30301 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    30361 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    30421 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    30481 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    30541 gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa
    30601 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    30661 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    30721 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    30781 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    30841 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    30901 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    30961 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    31021 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    31081 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    31141 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    31201 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    31261 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    31321 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    31381 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    31441 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    31501 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    31561 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    31621 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    31681 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    31741 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    31801 tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc
    31861 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    31921 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    31981 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    32041 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    32101 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    32161 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    32221 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    32281 tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    32341 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    32401 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    32461 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    32521 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    32581 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    32641 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    32701 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    32761 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    32821 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    32881 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    32941 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    33001 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    33061 ccaggtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    33121 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    33181 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    33241 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    33301 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    33361 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    33421 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    33481 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    33541 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    33601 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    33661 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    33721 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    33781 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    33841 gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg caaattaaag
    33901 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    33961 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    34021 tacatacccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    34081 tttgcgc
//
LOCUS       DADLCQ010000033        33529 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000033 DADLCQ010000000
VERSION     DADLCQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.033"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="JQG95_001428"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="JQG95_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY5888034.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="JQG95_001429"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="JQG95_001429"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY5888035.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQG95_001430"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="JQG95_001430"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY5888036.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQG95_001431"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="JQG95_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY5888037.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQG95_001432"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="JQG95_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY5888038.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /locus_tag="JQG95_001433"
     CDS             complement(3426..4094)
                     /locus_tag="JQG95_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY5888039.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="JQG95_001434"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="JQG95_001434"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY5888040.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="JQG95_001435"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="JQG95_001435"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY5888041.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="JQG95_001436"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="JQG95_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY5888042.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQG95_001437"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="JQG95_001437"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY5888043.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQG95_001438"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="JQG95_001438"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY5888044.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="JQG95_001439"
     CDS             8212..9075
                     /locus_tag="JQG95_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY5888045.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="JQG95_001440"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="JQG95_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY5888046.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="JQG95_001441"
     CDS             10411..11028
                     /locus_tag="JQG95_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY5888047.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQG95_001442"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="JQG95_001442"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY5888048.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="JQG95_001443"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="JQG95_001443"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY5888049.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQG95_001444"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="JQG95_001444"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY5888050.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="JQG95_001445"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="JQG95_001445"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY5888051.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="JQG95_001446"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="JQG95_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY5888052.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="JQG95_001447"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="JQG95_001447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY5888053.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQG95_001448"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="JQG95_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY5888054.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="JQG95_001449"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="JQG95_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY5888055.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="JQG95_001450"
     CDS             21859..23568
                     /locus_tag="JQG95_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY5888056.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQG95_001451"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="JQG95_001451"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY5888057.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="JQG95_001452"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="JQG95_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="JQG95_001453"
     tRNA            27625..27719
                     /locus_tag="JQG95_001453"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            28020..29204
                     /locus_tag="JQG95_001454"
     CDS             28020..29204
                     /locus_tag="JQG95_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5888058.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPREAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29616..30659
                     /locus_tag="JQG95_001455"
     CDS             29616..30659
                     /locus_tag="JQG95_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY5888059.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPENNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..31635
                     /locus_tag="JQG95_001456"
     CDS             31174..31635
                     /locus_tag="JQG95_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888060.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(31632..32487)
                     /locus_tag="JQG95_001457"
                     /pseudo
     CDS             complement(31632..32487)
                     /locus_tag="JQG95_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32484..32783)
                     /locus_tag="JQG95_001458"
     CDS             complement(32484..32783)
                     /locus_tag="JQG95_001458"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5888061.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            33245..33502
                     /locus_tag="JQG95_001459"
     CDS             33245..33502
                     /locus_tag="JQG95_001459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888062.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT         8401 a         8810 c         8263 g         8055 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcatgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtagatg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaaagccg gaggatgtca
    28021 tggcattaac cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caagggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaaaata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat
    31441 attgttacac cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg
    31501 ccagcgacct ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt
    31561 ggtgagattg ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg
    31621 tggacacggc cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag
    31681 gcactgtgac gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc
    31741 attatttccc ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag
    31801 aagctttcca cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg
    31861 ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta
    31921 tgaacaatga cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag
    31981 gccagttgtg ctgtcattcg cggtgacatc gaccagccaa tgacggagcg tgaccacagg
    32041 tcgatgacta ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc
    32101 gcccacttct ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac
    32161 gggcaggcca tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag
    32221 cccctgacga cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc
    32281 aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc
    32341 gcaggtgagc cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata
    32401 ccagccgctg cgggcaactc gaagtacacg acacatcgct ttgatgctga actctgcccg
    32461 atgattttcg ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt
    32521 tggaggatgg acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca
    32581 gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag
    32641 gcataaagct gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt
    32701 tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg
    32761 ctgattgata ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct
    32821 gtgtccacta ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat
    32881 atatcatctg taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt
    32941 gaatattata actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg
    33001 ttattatcac caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc
    33061 tttgtgacca aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta
    33121 ttttgctaca tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt
    33181 agttactcaa ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca
    33241 aagattgaaa aattatccaa cctatggttt ttattaccca atagccaatt ttatcgcatt
    33301 acaaataaat ggttcaaaaa caggatttgc cgcatctatt acaggtttta ctcaaacaat
    33361 aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc gacaacggtg ttggtcccgg
    33421 aactacttta ggtcttggtt cactaataac ttcattactg tcaatagccg taatcgccat
    33481 tgcaggaaat aataaaaagt aagtgtactc tgactgcgta tgtatctac
//
LOCUS       DADLCQ010000034        33519 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000034 DADLCQ010000000
VERSION     DADLCQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.034"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="JQG95_001460"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="JQG95_001460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY5888063.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2992..3921)
                     /locus_tag="JQG95_001461"
     CDS             complement(2992..3921)
                     /locus_tag="JQG95_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY5888064.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4138..4374)
                     /locus_tag="JQG95_001462"
     CDS             complement(4138..4374)
                     /locus_tag="JQG95_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY5888065.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQG95_001463"
     CDS             complement(4378..6279)
                     /gene="ipaA"
                     /locus_tag="JQG95_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY5888066.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQG95_001464"
     CDS             complement(6288..7286)
                     /gene="ipaD"
                     /locus_tag="JQG95_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY5888067.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLNILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLENLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQG95_001465"
     CDS             complement(7337..8428)
                     /gene="ipaC"
                     /locus_tag="JQG95_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY5888068.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQG95_001466"
     CDS             complement(8448..10190)
                     /gene="ipaB"
                     /locus_tag="JQG95_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY5888069.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQG95_001467"
     CDS             complement(10196..10663)
                     /gene="ipgC"
                     /locus_tag="JQG95_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY5888070.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JQG95_001468"
     CDS             complement(10720..11346)
                     /gene="ipgB"
                     /locus_tag="JQG95_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY5888071.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JQG95_001469"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="JQG95_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY5888072.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JQG95_001470"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="JQG95_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY5888073.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="JQG95_001471"
     CDS             13562..15178
                     /locus_tag="JQG95_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY5888074.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="JQG95_001472"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="JQG95_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY5888075.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="JQG95_001473"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="JQG95_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY5888076.1"
                     /translation="MSRFVFILLCFTPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="JQG95_001474"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="JQG95_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY5888077.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /gene="mxiH"
                     /locus_tag="JQG95_001475"
     CDS             17140..17391
                     /gene="mxiH"
                     /locus_tag="JQG95_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY5888078.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="JQG95_001476"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="JQG95_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY5888079.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /locus_tag="JQG95_001477"
     CDS             17703..18428
                     /locus_tag="JQG95_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY5888080.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="JQG95_001478"
     CDS             18425..18952
                     /locus_tag="JQG95_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY5888081.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /locus_tag="JQG95_001479"
     CDS             18894..19589
                     /locus_tag="JQG95_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY5888082.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /locus_tag="JQG95_001480"
     CDS             19582..19989
                     /locus_tag="JQG95_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY5888083.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="JQG95_001481"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="JQG95_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY5888084.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="JQG95_001482"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="JQG95_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY5888085.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="JQG95_001483"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="JQG95_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY5888086.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="JQG95_001484"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="JQG95_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY5888087.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="JQG95_001485"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="JQG95_001485"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY5888088.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spaK"
                     /locus_tag="JQG95_001486"
     CDS             26022..26423
                     /gene="spaK"
                     /locus_tag="JQG95_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY5888089.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26427..27719
                     /locus_tag="JQG95_001487"
     CDS             26427..27719
                     /locus_tag="JQG95_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY5888090.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28168
                     /gene="spaM"
                     /locus_tag="JQG95_001488"
     CDS             27812..28168
                     /gene="spaM"
                     /locus_tag="JQG95_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY5888091.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="JQG95_001489"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="JQG95_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY5888092.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGLFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spaO"
                     /locus_tag="JQG95_001490"
     CDS             29009..29890
                     /gene="spaO"
                     /locus_tag="JQG95_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY5888093.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spaP"
                     /locus_tag="JQG95_001491"
     CDS             29895..30545
                     /gene="spaP"
                     /locus_tag="JQG95_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY5888094.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="JQG95_001492"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="JQG95_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY5888095.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spaR"
                     /locus_tag="JQG95_001493"
     CDS             30820..31590
                     /gene="spaR"
                     /locus_tag="JQG95_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY5888096.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spaS"
                     /locus_tag="JQG95_001494"
     CDS             31597..32625
                     /gene="spaS"
                     /locus_tag="JQG95_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY5888097.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="JQG95_001495"
     CDS             32638..32880
                     /locus_tag="JQG95_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888098.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10460 a         4873 c         6709 g        11477 t
ORIGIN      
        1 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg ctgtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atataatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa atctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtggg ttttttgttt atcaccatgt
     2641 aaaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2701 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2761 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2821 gataattttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2881 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     2941 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3001 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3061 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3121 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3181 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3241 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3301 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3361 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3421 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3481 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3541 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3601 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3661 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3721 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3781 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3841 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3901 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     3961 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4021 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4081 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4141 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4201 taatgtagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4261 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4321 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4381 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4441 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4501 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4561 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4621 tgttacgtag tttcttatat ttgcatctgt aacttttaat ttgttgttta ttctatgttc
     4681 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4741 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4801 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat cagtctgaag
     4861 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4921 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     4981 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc cccgagaaag
     5041 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5101 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5161 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5221 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5281 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5341 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5401 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5461 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5521 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5581 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5641 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5701 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5761 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5821 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5881 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     5941 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6001 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6061 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6121 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6181 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6241 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6301 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6361 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6421 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6481 acctcgccat ttccacctag attctctaag ctttttaaca tattgtctat tggggtcatg
     6541 tttatactga caacatatcc cccgtttttt tgagatacct tgccgattgt tccacctaat
     6601 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6661 ttatctttat atttttcctt gagttcttcc aatgcctttt taagcgagtt gacttgtaat
     6721 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6781 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6841 tactgttcat taatattatt gatggagttt gcaattttag cccacagttc tctatgagat
     6901 atcatttgat ctccatcaag ctcggcttct ttcggagctg aatgtaataa ttctcttgcg
     6961 tctttattaa ttggatattc gttcctggaa agaatattca atagttgagc ggatttattt
     7021 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7081 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7141 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7201 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7261 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7321 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7381 ttgattggtt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7441 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7501 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7561 cagcaacggc tgatgtttcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7621 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7681 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7741 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7801 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7861 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7921 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     7981 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8041 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8101 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8161 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggcgagca atgatttctg
     8221 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8281 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8341 atttttgtgt ttcggaagaa ctttgagttt gttttgtgga tatatctgta tataaaatct
     8401 gggttggttt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8461 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8521 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8581 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8641 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8701 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8761 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8821 agttgagaag aaaaattctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8881 attttgccaa tattttctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     8941 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9001 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9061 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9121 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9181 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9241 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9301 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9361 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9421 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9481 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9541 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9601 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9661 tttttctttt ctggtgactc tgggtcgagt tcggataatc ttgtttgaat ttggttaatt
     9721 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9781 tagtctctgg ttagtccttc agtttcagat agaagagtgt taattttatc ggagaattct
     9841 aggttttttt gctgtcttgc ctgttgctgg gacttccaag cagtaatttt atttgttaat
     9901 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
     9961 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10021 tgtgcattag ttgtattaat attttggtta gcagtaagat cagcaattgt aagagaaaat
    10081 aatttgttag ctgcatcatt tgcagcttgg atagtattgt ctccaagctc agtggaagcc
    10141 aatattttgg caagaggaaa accagtggtt gtggtgctta cattatgcat aataattact
    10201 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10261 tgctgtgttg aattacgagt tcgaagcact ctttagcttt taagggggct ttcaaccgaa
    10321 gctggcattg tccagtatgg aatactggtg tatagtcatt ttttcctaat gcaaaagcga
    10381 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10441 ccataatgta gtctacattg taaaagtcgt atatacataa aaacctgaag aaaacttcag
    10501 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10561 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10621 ctgcagtaga gatgctttca ttttcggtga tatttaaaga cataaggtct cctttttgtc
    10681 tgctttactt taataagtat aagatttaat ataaaagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tatagcgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataagt
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgcgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcat acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgatctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caataatttt
    11881 tattgatttt tcgatgattt cttctttttt atctgttgct tcggttttta attcgtttaa
    11941 tacacgagtc atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttggtg tgtggataag gttaatttta ttgaaacttt taagatagct
    12121 gcgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatttc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 atcgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt ctttttgcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatggctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgc ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact agtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaaaacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 acgctctgcg gtattcaaca ttaatttgat gtctctacct gtgaaagagt gttgaattga
    12961 gtttttagct gcttcatagg cggcaaacgc actttctgca gatttttcgc tagctaatgt
    13021 attgaggaaa agcaataagg atttttgatt ctctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta tcaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg aggatcatac tttattaact
    13261 ctccattact tggtgattgt ggggatgtaa aatgtatttg gtatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctcgttt catcatgaaa tcccacaaga taaagtgcct
    13441 gatgtatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa agcacaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagaa agaaataagg ggatcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca ggaaaaagcc ttttgtttgc gcgtgacaga gctgaagttt tttgcgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac gattagatac
    13861 ctataaactt ggtgaagtta ataaaagaca tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaaacga aaataaaaca
    13981 aatatttact gattatttaa ataataagaa ttggggaccg gtcgacaaga atattagtca
    14041 tcatgggaaa aattatggtt ttcaattaac tcctgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 tgatcatatc gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgct gctcgtaata aagctgagga gttagtaagc gcagcattat atagcagacc
    14341 agagttatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taaccggggg agaggagagt atgttaaaag atcaggtgaa
    14461 cgcattaaaa gggttaaact ctaaaagggg ggagccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgaaagaag taagcgttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatgatga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagca atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attgtaacca aaaagttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atgactctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca ggtcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcccggaaat aaagtcatga aaaaattgcc gctatcttcg ttagagctat cttattctga
    15121 aagaataggg gacccaaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc ctttaattga
    15241 tatcgatgat caagcaatca tgcttgatga tgatgtgcta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgctta gctactgatg atgaaggcgg
    15421 aaatctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggatg tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt ttgctgtgtt ttaccccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaatattc catcaagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tctgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tattctgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgatgat gtatggaaga gggtgggagg ctgttggagc ctataatgca
    15901 ggaacgtctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctatagacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gacaatttcc cagaaaacat tattcctgta acagatagta
    16201 aatcggatgg tataatttat ttgaccataa gcaaagataa tatctgtcaa tttagtgatg
    16261 aaaaaggtga gcaaattgat atcaattcac aatttaattc atttgaatat gacgggattt
    16321 cttttcacct taaaaatatg agggaggata aatcgagagg ccatatacta aacggaatgt
    16381 ataagaacca ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 ccctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacactgga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcgtc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 caaacaaaga aataaataga attcaacaat atatcaatca acgattccca tttatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtgttttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgata aattgaaaaa tgctctgatt gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg ggggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaaat aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgaacataa aaatatatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccgaat
    17161 gatgattgga cattgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 ggtgaactaa cattggcact agataaatta gctaaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atccaataca
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagtct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaagt taaatcggaa tttttaacaa atgaagtaat atatttattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttggtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggagg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg tgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgcaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgcttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttcagctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaactg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagcccaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatatagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggtgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc gtcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggtgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaatt ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaataat atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataaaagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaacgttaa
    23161 aagataacct tcctctagat cgggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggaac ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatt attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gacgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga agtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agataaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtccctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataaatgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatatt ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttag atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta attttgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tggggtctta taatcaatga gctatacaaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tgttgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acagataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtattaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttactga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttagaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagacaa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggctc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcagtc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgactcattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgcactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagaaca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 tcttttagaa aaaaatatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgacatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtacct gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc tcaaaaattt gatacaatat ctgatattgt ccgtttttct gattctggct
    30181 tgatggaata taagcaatac ttgaaaaagc atacagatct tgagttagca cgttttttcc
    30241 aaaggtctga agaagaaaat gctgatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaatcaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttgtt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtggggtatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat tatctattgg
    30661 tttgttacag acagtgactc aactccaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt agctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggccttct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggtat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tcttaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggcgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgactgttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg gaggctgttt aaggatataa tattattttt cttggcattt
    32161 tctattcttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttattttaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgcactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgatcatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa gcccttgcgg
    33421 ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc cgtatgctga
    33481 cgaaatatcc cggctcttaa aacgatagtg gctcaggct
//
LOCUS       DADLCQ010000035        32634 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000035 DADLCQ010000000
VERSION     DADLCQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.035"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..956)
                     /locus_tag="JQG95_001496"
     CDS             complement(<1..956)
                     /locus_tag="JQG95_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY5888099.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /locus_tag="JQG95_001497"
     CDS             1202..1828
                     /locus_tag="JQG95_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY5888100.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..1980)
                     /locus_tag="JQG95_001498"
     CDS             complement(1825..1980)
                     /locus_tag="JQG95_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY5888101.1"
                     /translation="MSVNGDKVTEDGRILTDRGQRIRDVRTGPDGYLYVLTDESSGEL
                     LKVSPRN"
     gene            complement(2001..2939)
                     /locus_tag="JQG95_001499"
     CDS             complement(2001..2939)
                     /locus_tag="JQG95_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY5888102.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGS"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQG95_001500"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="JQG95_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY5888103.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="JQG95_001501"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="JQG95_001501"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY5888104.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="JQG95_001502"
     CDS             complement(5018..6346)
                     /locus_tag="JQG95_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5888105.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLFGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8702)
                     /locus_tag="JQG95_001503"
     CDS             complement(6354..8702)
                     /locus_tag="JQG95_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5888106.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="JQG95_001504"
     CDS             complement(8880..9791)
                     /gene="gsiD"
                     /locus_tag="JQG95_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY5888107.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="JQG95_001505"
     CDS             complement(9794..10714)
                     /gene="gsiC"
                     /locus_tag="JQG95_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY5888108.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="JQG95_001506"
     CDS             complement(10732..12270)
                     /gene="gsiB"
                     /locus_tag="JQG95_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY5888109.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="JQG95_001507"
     CDS             complement(12290..14161)
                     /gene="gsiA"
                     /locus_tag="JQG95_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY5888110.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="JQG95_001508"
     CDS             complement(14148..15113)
                     /gene="iaaA"
                     /locus_tag="JQG95_001508"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY5888111.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15317..16552
                     /gene="moeA"
                     /locus_tag="JQG95_001509"
     CDS             15317..16552
                     /gene="moeA"
                     /locus_tag="JQG95_001509"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY5888112.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16552..17301
                     /gene="moeB"
                     /locus_tag="JQG95_001510"
     CDS             16552..17301
                     /gene="moeB"
                     /locus_tag="JQG95_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY5888113.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVASARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="JQG95_001511"
     CDS             complement(17480..18142)
                     /gene="fsa"
                     /locus_tag="JQG95_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY5888114.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18273..19172
                     /locus_tag="JQG95_001512"
     CDS             18273..19172
                     /locus_tag="JQG95_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY5888115.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19178..21610
                     /locus_tag="JQG95_001513"
     CDS             19178..21610
                     /locus_tag="JQG95_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY5888116.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21756..22571
                     /gene="ybiV"
                     /locus_tag="JQG95_001514"
     CDS             21756..22571
                     /gene="ybiV"
                     /locus_tag="JQG95_001514"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY5888117.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22723..23988
                     /locus_tag="JQG95_001515"
     CDS             22723..23988
                     /locus_tag="JQG95_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY5888118.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24229..25821)
                     /locus_tag="JQG95_001516"
     CDS             complement(24229..25821)
                     /locus_tag="JQG95_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY5888119.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26040..26960
                     /gene="ldtB"
                     /locus_tag="JQG95_001517"
     CDS             26040..26960
                     /gene="ldtB"
                     /locus_tag="JQG95_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5888120.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27018..28136)
                     /locus_tag="JQG95_001518"
     CDS             complement(27018..28136)
                     /locus_tag="JQG95_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY5888121.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="JQG95_001519"
     CDS             complement(28133..28600)
                     /gene="mntR"
                     /locus_tag="JQG95_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HAY5888122.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28786..28914
                     /gene="mntS"
                     /locus_tag="JQG95_001520"
     CDS             28786..28914
                     /gene="mntS"
                     /locus_tag="JQG95_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY5888123.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29186..30769
                     /locus_tag="JQG95_001521"
     CDS             29186..30769
                     /locus_tag="JQG95_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY5888124.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="JQG95_001522"
     CDS             complement(30817..31332)
                     /gene="ompX"
                     /locus_tag="JQG95_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY5888125.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31385..31450)
                     /locus_tag="JQG95_001523"
     CDS             complement(31385..31450)
                     /locus_tag="JQG95_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888126.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31685..32572
                     /gene="rhtA"
                     /locus_tag="JQG95_001524"
     CDS             31685..32572
                     /gene="rhtA"
                     /locus_tag="JQG95_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY5888127.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         8166 a         8701 c         7884 g         7883 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattactgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaaaca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttcacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatcttta tcttttcgta taaactcagc
     8701 atgttaatac atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct
     8761 tgctgcggct ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact
     8821 tgcttagggc aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac
     8881 tatcctttaa ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc
     8941 agtacggtca gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata
     9001 tccgctcgtg cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg
     9061 ccgagaaatg acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac
     9121 accacgatag aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg
     9181 gcaccaatac tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg
     9241 cgcaccaggc gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca
     9301 atgccgcttc ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac
     9361 agcacatcgc aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac
     9421 aagcccagca acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc
     9481 gagatttgcg caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca
     9541 aaccagtgct gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg
     9601 tcatagggag cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat
     9661 gcggcggtca tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta
     9721 cgtacctggt caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta
     9781 aatagtcgca tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca
     9841 ccactaagtt gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg
     9901 ggtaatcgcg catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa
     9961 ctttctccac cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca
    10021 ctaccgggat catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc
    10081 ctttcgccct cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg
    10141 taaagcgcgc catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt
    10201 gctgccaact gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa
    10261 ttaaaaacat ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac
    10321 tcaatcgatc cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa
    10381 atatcaccgc ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg
    10441 caatctcatc ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag
    10501 cattgctgat atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac
    10561 gcaccagttc tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc
    10621 cgggcagcat gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca
    10681 accccagtaa tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa
    10741 atccgcgtct tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt
    10801 actgtgtgcc gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat
    10861 gatatcctgc gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc
    10921 ctgagccagg aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg
    10981 tggccagttt tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc
    11041 cgaccagcca gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc
    11101 tgccgcccgc tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa
    11161 ctgctgctgg gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag
    11221 cgtggtactg aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg
    11281 atcgtaaggc cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt
    11341 agctggcgtt gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta
    11401 attcagcgcc tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat
    11461 ataacgctgc ataattgacg gactggccat caactcgata tttttgtttt tctccagcag
    11521 tgtggcctgc tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc
    11581 cgcgcgggtg ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc
    11641 tggctgccag taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc
    11701 cagttcatac ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag
    11761 cgctgtcggt gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa
    11821 cggctgtttg agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt
    11881 cttatacagg ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc
    11941 ttttaccgcc actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt
    12001 cacggtgtaa gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag
    12061 tttcatctct ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga
    12121 taacgtgtca ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac
    12181 cacatctttg gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac
    12241 taacccacta cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca
    12301 tgaatgcgta ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg
    12361 agacggctgc cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca
    12421 gtacacgctg cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac
    12481 gcgtataagg atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt
    12541 gcccgagata catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg
    12601 agataaacag atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct
    12661 gcccgcgaat agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat
    12721 tcaatgccaa cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg
    12781 ggtaacgcca ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg
    12841 cagccgcgtc tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac
    12901 cgatggtctg acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc
    12961 ggcgcaatgc ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa
    13021 tttcgccgcc ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc
    13081 tgccagactc acccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct
    13141 caacggcatg cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg
    13201 tgacaagatt acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg
    13261 ggggttcctg tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta
    13321 accctttcat cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt
    13381 gcggtgcatg aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca
    13441 ccagtacccg atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc
    13501 ccatcgacat ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga
    13561 catccagcgc ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca
    13621 tcgcaatcat cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa
    13681 tggtttgtgc ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt
    13741 cttcacgact ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa
    13801 atactgggtt cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca
    13861 catggcgcat ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc
    13921 gcaacagcat tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg
    13981 ccagcgcagt cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc
    14041 gttgcagact aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg
    14101 caatattaag attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca
    14161 acggtgtccc ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag
    14221 gcgcgataca ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg
    14281 attaagccac cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct
    14341 tccgcgagac ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg
    14401 cggatgaaga cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag
    14461 catccggcac ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg
    14521 cctgtggacg tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg
    14581 cccattttct gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc
    14641 tcgcgcgctg ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg
    14701 cgctccatgc catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc
    14761 gggctttgct ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc
    14821 gccaccgcac cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt
    14881 tcatcacgcg taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga
    14941 cgcaccgctt ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg
    15001 gtttcaacaa tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc
    15061 gcgcggctaa ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc
    15121 gtccattaag ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca
    15181 ttcaattttt gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc
    15241 ccgaggtggc atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct
    15301 ccgcaggagt gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa
    15361 tgagatgctt tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg
    15421 ttttggtcgt attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa
    15481 ctccgctatg gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc
    15541 cgttgccggt aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg
    15601 cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga
    15661 gcagactgaa caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa
    15721 tattcgccgt cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg
    15781 cctgactacc gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat
    15841 tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc
    15901 gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca
    15961 gctgggttgc gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc
    16021 cgcatttatt gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt
    16081 gggtgaagcg gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa
    16141 gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg
    16201 cggcctgccg ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt
    16261 gctggcaaaa ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac
    16321 agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg
    16381 caacgccgac ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag
    16441 ctcctttagt ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt
    16501 gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa
    16561 ctcagcgatc aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt
    16621 gacggtcagg aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc
    16681 tgcgcagctt cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc
    16741 gacacggttt cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg
    16801 caaccgaagg tggcatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt
    16861 acgccagtca atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat
    16921 ctggtgctcg actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt
    16981 gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc
    17041 ttcacttatc aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac
    17101 gcgttaacct gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg
    17161 caggcaatgg aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc
    17221 gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg
    17281 tgtgaggtgt gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc
    17341 atcaggcatt tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg
    17401 ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat
    17461 gaggatgcag aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct
    17521 caaacttcgt cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca
    17581 gtggcagagt aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt
    17641 tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt
    17701 cggtcacagt ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa
    17761 catattccgc acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc
    17821 ccagcgtcgg aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca
    17881 ccggaacttt caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca
    17941 tcccttcagc agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt
    18001 catgaagttg cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt
    18061 tagtggtcac acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg
    18121 aagtatccag atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac
    18181 tatcacggga aaactctctg aacagttgac gcacatcaat ctgactttca ttcgaaagta
    18241 ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga
    18301 cccatgatgg ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc
    18361 gctggtgtca gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac
    18421 tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc
    18481 tgaatggttt gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg
    18541 actgctgtcc gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca
    18601 tggcgaccgt tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt
    18661 cgggtggtga acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg
    18721 aggcaggcat tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc
    18781 cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt
    18841 ttaaacagtg gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg
    18901 cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa
    18961 cctctgtaaa agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt
    19021 ttctgcccta ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg
    19081 ccccggaaaa accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc
    19141 agaaagggtt aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac
    19201 gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt
    19261 ctgtaccgag cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat
    19321 cccggtacgt cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa
    19381 acacgatgag ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc
    19441 ggaatatact gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc
    19501 tggctttgcg gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc catggtggcg
    19561 cggtcagacg gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct
    19621 ggcgaccggc atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt
    19681 caatttcccg ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg
    19741 tcgctcgcgc atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc
    19801 gattgatatc gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg
    19861 tgcaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga
    19921 aaactgcgat cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg
    19981 ttacttcatc cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg
    20041 tatggatcag tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga
    20101 tcgcgaacac gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa
    20161 aatccgctcc ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac
    20221 cattggcgga caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta
    20281 cgcgatcctc gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta
    20341 ccatgcagga atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt
    20401 cgggatgccg gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat
    20461 tgaaccgcag gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg
    20521 caaatggggc tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc
    20581 ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc
    20641 gttgtcggca ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat
    20701 ccgttactac acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga
    20761 gaacgtgcac gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag
    20821 tatcaagcaa ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa
    20881 cctcggcaac agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca
    20941 gcaacagctg gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg
    21001 tcagcggctg attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct
    21061 gctggctcgc gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta
    21121 cggtcgtggt ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt
    21181 accgtttggc gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc
    21241 agaaggcgct agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc
    21301 agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa
    21361 tccggcaacg ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt
    21421 ctttgaagtg cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct
    21481 ggaggcgaaa aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc
    21541 cgccttcttc accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca
    21601 tatgctgtaa tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca
    21661 atttcatcct tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac
    21721 tgttacggtt tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag
    21781 atatggacgg tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc
    21841 aatatcagga actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt
    21901 accagcttat ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg
    21961 gcgcactggt ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat
    22021 cgcggattgt tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc
    22081 tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac
    22141 actaccatcg cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt
    22201 tttcgctcaa cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc
    22261 tggatggcat tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg
    22321 gtctacataa agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa
    22381 atgtggtagc gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt
    22441 cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg
    22501 ataataatca tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt
    22561 ttaacagctg acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct
    22621 gtacatcaca tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg
    22681 caaattagca tagtttcgaa tgaaagttaa tgcgaggtgg ttatggcttc tacttttacc
    22741 agcgacacat tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg
    22801 gcgcagcttg gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga
    22861 gtggctgaaa tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat
    22921 gccgatatca aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt
    22981 tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg
    23041 ctggactatc tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc
    23101 ttcgggacgc tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa
    23161 atgcaggccc gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg
    23221 acatttgaaa gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac
    23281 cgtatccgcc gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc
    23341 ggggcgctgg aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat
    23401 ggcaatctgg cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac
    23461 acggtggaca acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc
    23521 tcggatatgc tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg
    23581 tacgtactgt tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg
    23641 cccggaagag tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc
    23701 agcattccaa aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc
    23761 gttgcccccg ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg
    23821 atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca
    23881 tcgccgggcg acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt
    23941 gccgacctca acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa
    24001 gtgaattgat gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg
    24061 ctttcttaat gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc
    24121 aatatattac aggtactgcg taggcctgat aaacgtagcg catcaggcta ttttgcattt
    24181 gtcatcagcc gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc
    24241 tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat
    24301 ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag
    24361 cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg
    24421 gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag
    24481 catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct
    24541 gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca
    24601 ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc
    24661 gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc
    24721 gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag
    24781 tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc
    24841 tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg
    24901 gataaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg
    24961 gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa
    25021 agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg
    25081 cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc
    25141 gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat
    25201 gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat
    25261 gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg
    25321 cgccagaagc acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg
    25381 ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc
    25441 gtccatttcg ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat
    25501 ttccggcaaa gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc
    25561 catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt
    25621 accaatgcgc tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc
    25681 gagaatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt
    25741 gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt
    25801 gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta
    25861 tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc
    25921 aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg
    25981 cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca
    26041 tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta
    26101 ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc
    26161 aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc
    26221 agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag
    26281 gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca
    26341 tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta
    26401 tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca
    26461 aagttgagca taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt
    26521 accgcgctgc gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg
    26581 gtctgtatgc actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg
    26641 gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc
    26701 tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga
    26761 ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag
    26821 cccagtttga aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga
    26881 ccggtcagcc agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga
    26941 tgccggttcg cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct
    27001 tttttatttg ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc
    27061 gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg
    27121 ttcgccatac gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc
    27181 acgtttaccg cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta
    27241 ctcggcacgt tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca
    27301 gataaatgac tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg
    27361 acgtcgataa acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg
    27421 cgacgcgcca gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg
    27481 aactccagcg ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt
    27541 ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac
    27601 cagcacaaca gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata
    27661 aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt
    27721 ggcgtcagta gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc
    27781 gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca
    27841 tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga
    27901 cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac
    27961 tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata
    28021 gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga
    28081 attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg
    28141 caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct
    28201 ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat
    28261 tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta
    28321 aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca
    28381 ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt
    28441 cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc
    28501 gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg
    28561 tcactttttt tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg
    28621 acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa
    28681 aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg tatgtaatgc
    28741 aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag
    28801 aggtgtatgc gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg
    28861 ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt
    28921 cgcggtacgc cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa
    28981 accacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc
    29041 tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg
    29101 acccctttcc cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac
    29161 atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg
    29221 tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct
    29281 cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt
    29341 gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc
    29401 ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc
    29461 attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag
    29521 ctacctggtc ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga
    29581 gaatgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac
    29641 attttttgtt gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac
    29701 tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga
    29761 agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt
    29821 taagccgaag tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct
    29881 cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat
    29941 atttgctgac tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa
    30001 tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc
    30061 gggcttccag acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat
    30121 cgccagcatc gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc
    30181 agataaaaac acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga
    30241 gcactcgcaa ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga
    30301 caggacacaa ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta
    30361 taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg
    30421 cagcagcttc tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa
    30481 agacgtgcaa taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat
    30541 ggtcatttcc agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga
    30601 cttcttaggc tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata
    30661 cccgtttata tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa
    30721 ggtggattac aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc
    30781 cgccccgaga ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc
    30841 aatccaagtg cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc
    30901 aacgttttcc atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt
    30961 gtcgtgtttg taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac
    31021 accgtagatg cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta
    31081 ctggtttttg ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga
    31141 accgatcaca cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc
    31201 cattttgttc atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt
    31261 cgcagctacg gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc
    31321 aatttttttc ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca
    31381 aaaattaatg ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga
    31441 acgtttccat acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag
    31501 tattacgcat gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt
    31561 ccatcaatcc tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac
    31621 cagcattggg tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga
    31681 aaggatgcct ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt
    31741 tgccatggcg tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag
    31801 cgcaccgggt gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt
    31861 taagccatgg cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt
    31921 ttcgctgggt gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat
    31981 tgcggtggcg ctggagttca ccggaccact ggcggtggca ctgttctctt ctcgtcgccc
    32041 ggtagatttc gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg
    32101 gcaagacgtt tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg
    32161 ttgggctatt tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt
    32221 agcaattggt tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg
    32281 tgaagcactc tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac
    32341 cgctctgcct tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg
    32401 tacgctgatg agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga
    32461 aacactgaca cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc
    32521 tacgctgaca gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat
    32581 ttctgtatgg ttatgcataa ccatacagaa tttctcgcta cttttcctct acac
//
LOCUS       DADLCQ010000036        32143 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000036 DADLCQ010000000
VERSION     DADLCQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.036"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..956
                     /locus_tag="JQG95_001525"
                     /pseudo
     CDS             1..956
                     /locus_tag="JQG95_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1128..1235
                     /locus_tag="JQG95_001526"
     CDS             1128..1235
                     /locus_tag="JQG95_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888128.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="JQG95_001527"
     CDS             complement(1288..2046)
                     /locus_tag="JQG95_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY5888129.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2333..3262
                     /gene="pfkB"
                     /locus_tag="JQG95_001528"
     CDS             2333..3262
                     /gene="pfkB"
                     /locus_tag="JQG95_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY5888130.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3363..3653
                     /gene="ghoS"
                     /locus_tag="JQG95_001529"
     CDS             3363..3653
                     /gene="ghoS"
                     /locus_tag="JQG95_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY5888131.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3759..4619
                     /locus_tag="JQG95_001530"
     CDS             3759..4619
                     /locus_tag="JQG95_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY5888132.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4660..5196)
                     /locus_tag="JQG95_001531"
     CDS             complement(4660..5196)
                     /locus_tag="JQG95_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888133.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5343..6011
                     /gene="hxpB"
                     /locus_tag="JQG95_001532"
     CDS             5343..6011
                     /gene="hxpB"
                     /locus_tag="JQG95_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY5888134.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6174..6764
                     /locus_tag="JQG95_001533"
     CDS             6174..6764
                     /locus_tag="JQG95_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5888135.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6897..8287
                     /locus_tag="JQG95_001534"
                     /pseudo
     CDS             6897..8287
                     /locus_tag="JQG95_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(8291..9107)
                     /locus_tag="JQG95_001535"
                     /pseudo
     CDS             complement(8291..9107)
                     /locus_tag="JQG95_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="JQG95_001536"
     CDS             complement(9384..9647)
                     /gene="cedA"
                     /locus_tag="JQG95_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY5888136.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9830..12091
                     /gene="katE"
                     /locus_tag="JQG95_001537"
     CDS             9830..12091
                     /gene="katE"
                     /locus_tag="JQG95_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY5888137.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLKPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="JQG95_001538"
     CDS             complement(12138..12896)
                     /gene="chbG"
                     /locus_tag="JQG95_001538"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY5888138.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPVNLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12909..14261)
                     /locus_tag="JQG95_001539"
     CDS             complement(12909..14261)
                     /locus_tag="JQG95_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY5888139.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTETVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGHDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14366..15205)
                     /gene="chbR"
                     /locus_tag="JQG95_001540"
                     /pseudo
     CDS             complement(14366..15205)
                     /gene="chbR"
                     /locus_tag="JQG95_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="JQG95_001541"
     CDS             complement(15216..15566)
                     /gene="chbA"
                     /locus_tag="JQG95_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY5888140.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="JQG95_001542"
     CDS             complement(15625..16974)
                     /gene="chbC"
                     /locus_tag="JQG95_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAY5888141.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="JQG95_001543"
     CDS             complement(17059..17379)
                     /gene="chbB"
                     /locus_tag="JQG95_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY5888142.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="JQG95_001544"
     CDS             complement(17678..18016)
                     /gene="osmE"
                     /locus_tag="JQG95_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY5888143.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18218..19045
                     /gene="nadE"
                     /locus_tag="JQG95_001545"
     CDS             18218..19045
                     /gene="nadE"
                     /locus_tag="JQG95_001545"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY5888144.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            19275..20162
                     /gene="cho"
                     /locus_tag="JQG95_001546"
     CDS             19275..20162
                     /gene="cho"
                     /locus_tag="JQG95_001546"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY5888145.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20122..20697)
                     /gene="ves"
                     /locus_tag="JQG95_001547"
     CDS             complement(20122..20697)
                     /gene="ves"
                     /locus_tag="JQG95_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY5888146.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(20900..21385)
                     /gene="spy"
                     /locus_tag="JQG95_001548"
     CDS             complement(20900..21385)
                     /gene="spy"
                     /locus_tag="JQG95_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY5888147.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(21715..22683)
                     /gene="astE"
                     /locus_tag="JQG95_001549"
     CDS             complement(21715..22683)
                     /gene="astE"
                     /locus_tag="JQG95_001549"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY5888148.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALVCTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(22676..24009)
                     /gene="astB"
                     /locus_tag="JQG95_001550"
                     /pseudo
     CDS             complement(22676..24009)
                     /gene="astB"
                     /locus_tag="JQG95_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(24006..25484)
                     /gene="astD"
                     /locus_tag="JQG95_001551"
     CDS             complement(24006..25484)
                     /gene="astD"
                     /locus_tag="JQG95_001551"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY5888149.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTVGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(25481..26515)
                     /gene="astA"
                     /locus_tag="JQG95_001552"
     CDS             complement(25481..26515)
                     /gene="astA"
                     /locus_tag="JQG95_001552"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY5888150.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERTI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(26512..27732)
                     /gene="astC"
                     /locus_tag="JQG95_001553"
                     /pseudo
     CDS             complement(26512..27732)
                     /gene="astC"
                     /locus_tag="JQG95_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            28178..28984
                     /gene="xthA"
                     /locus_tag="JQG95_001554"
     CDS             28178..28984
                     /gene="xthA"
                     /locus_tag="JQG95_001554"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY5888151.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            29151..29859
                     /locus_tag="JQG95_001555"
                     /pseudo
     CDS             29151..29859
                     /locus_tag="JQG95_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
     gene            29864..30541
                     /gene="ydjY"
                     /locus_tag="JQG95_001556"
     CDS             29864..30541
                     /gene="ydjY"
                     /locus_tag="JQG95_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY5888152.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            30556..31263
                     /locus_tag="JQG95_001557"
     CDS             30556..31263
                     /locus_tag="JQG95_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY5888153.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            31263..31811
                     /locus_tag="JQG95_001558"
     CDS             31263..31811
                     /locus_tag="JQG95_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY5888154.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            31821..>32143
                     /locus_tag="JQG95_001559"
     CDS             31821..>32143
                     /locus_tag="JQG95_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY5888155.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
BASE COUNT         8247 a         8240 c         7612 g         8044 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttccagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattaacca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatatatt tatctgcaaa attttaaata aagctctaat aaatcatatt gttaatttct
     2281 tcactttccg ctgattcggt gccagactga aatcagccta taggaggaaa tgatggtacg
     2341 tatctatacg ttgacacttg cgccctctct cgatagcgca acaattaccc cgcaaattta
     2401 tcccgaagga aaactgcgct gtaccgcacc ggtgttcgaa cccgggggcg gcggcatcaa
     2461 cgtcgcccgc gccattgccc atcttggagg cagtgccaca gcgatcttcc cggcgggtgg
     2521 cgcgaccggc gaacacctgg tttcactgtt ggcggatgaa aatgtccccg tcgctactgt
     2581 agaagccaaa gactggaccc ggcagaattt acacgtacat gtggaagcaa gcggtgaaca
     2641 gtatcgtttt gttatgccag gcgcggcatt aaatgaagat gagtttcgcc agcttgaaga
     2701 gcaagttctg gaaattgaat ccggggccat cctggtcata agcggaagcc tgccgccagg
     2761 tgtgaagctg gaaaaattaa cccaactgat ttccgctgcg caaaaccaag ggatccgctg
     2821 catcatcgac agttctggcg aagcgttaag tgcagcactg gcgattggta acatcgagtt
     2881 ggttaagcct aaccaaaaag aactcagtgc gctggtgaat cgcgaactca cccagccaga
     2941 cgatgtccgc aaagccgcgc aggaaatcgt taatagcggc aaggccaaac gggttgtcgt
     3001 ttcccttggt ccacaaggag cgctgggtgt cgatagtgaa aactgcattc aggtggtgcc
     3061 accaccggtg aaaagccaga gtaccgttgg cgctggtgac agcatggtcg gcgcgatgac
     3121 actgaaactg gcagaaaatg cctctcttga agagatggtc cgttttggcg tagctgcggg
     3181 gagtgcagcc acactcaatc agggaacacg tctgtgctcc catgacgata cgcaaaaaat
     3241 ttacgcttac ctttcccgct aacaaaaaca ttcccccagc attgggggaa tcaccaccaa
     3301 cctgtcggca acgcgtttct ccgactatgc tcaaaagtca tgtgataaca aaggggtgaa
     3361 ctatggccag tggcgatctt gtccgttatg tcataaccgt catgttgcat gaggatacat
     3421 tgactgaaat taacgagttg aataattacc tgactcgcga cggttttttg ctcaccatga
     3481 cggatgatga gggaaatatc catgagctgg ggactaacac ttttggactt atcagtaccc
     3541 aaagtgaaga agaaattaga gaactggttt cggggcttac ccaaagtgca accggcaaag
     3601 atcctgaaat caccatcacg acctgggagg aatggaatag caacagaaaa taaatggttt
     3661 ttgggcaata atcagtctgt ggtgtgcgtt agctcgtgtt tttacaccgc attcttgcgc
     3721 taaccttatg atctggcaga caacatggga gagacatcat gtggcaggca atcagtcgtc
     3781 ttttgagcga gcagttaggt gaaggcgaaa tcgaactgcg taatgaactg cctggcggag
     3841 aagtccatgc cgcatggcat ttgcgctatg caggacatga ctttttcgtc aaatgtgatg
     3901 aaagggaact gcttcccggt tttaccgccg aagccgacca actggagtta ctgtcgcgta
     3961 gtaaaaccgt caccgtgcct aaggtttggg cagtaggcgc tgaccgtgac tacagttttc
     4021 tggtgatgga ttatctccca cctcgtccgc tggatgcgca tagcgcattt attcttggtc
     4081 agcaaattgc gcgtttacat caatggagtg accaaccaca atttggcctc gattttgata
     4141 actcgctctc cacaactcca cagcccaaca cctggcaacg tcgctggtca acgttttttg
     4201 ctgaacaacg gattggctgg cagttggaac tggcagcaga gaaagggatc gctttcggca
     4261 atatcgacgc catcgtcgag catattcagc agcgtctggc ctcgcatcaa ccgcagcctt
     4321 ctctgttgca cggcgattta tggtccggca actgtgcgct gggtccggat ggcccgtaca
     4381 ttttcgaccc tgcctgcgac tggggtgacc gagagtgcga cctagcgatg ttaccgctgc
     4441 atactgaaca gccgccacaa atctatgacg gctatcagtc agtatccccg ctacctgccg
     4501 atttccttga acgtcaaccg gtttaccaac tctacacgct gttaaatcgt gcaagattat
     4561 ttggcggtca gcatttggtt attgctcagc agtcattgga tagattatta gcagcatgat
     4621 atgggttgag gataatggcc gctccgtgcg gccttttgat taaataaacc caagcagaga
     4681 aaagaacaca taacctgcag caatacaaat aaccggcagg atatatagtg agaaaaactg
     4741 caggaaaata gtatggtgcg gaacaacaat acgggactca atttgttcac gcgttagccc
     4801 ctcttcccct ttggcttttt ccagaatgag ttgatcttca acaccttccc gcaagaagcg
     4861 cgcctggcga ctcatccggg caccggaatc ctgcaacgcc agtccgacaa aaatcaggat
     4921 gaaaatcacc cagaacataa cgttcacacc accattaaaa ttaggggtcg gggagttata
     4981 ccagaacaga ttcaaaaaag gcgtattcgc ctgcatcata tcgatcatga catgagcaaa
     5041 gtcgagcatg accgcattaa tgccttcctg gttttcctgc cgggtattca taaatttcag
     5101 caaagaaatc aatgtagaga tcaatgcagg tataaaaatc acccaaccca aaatcctttt
     5161 caaaacagca atgcgtccag cttgttgata cgtcatgagt tctccttgat taagacgcgt
     5221 cgtttcactt agtttacctg tagatatctg ttttcgccca ttctttaaag gcgatatgat
     5281 aggcgcttaa tcataagcac ggcttaatac cttacacata atgctctaaa ggagaggttg
     5341 taatgtcaac cccgcgtcag attcttgctg caatttttga tatggatgga ttacttatcg
     5401 actcagaacc tttatgggat cgagccgaac tggatgtgat ggcaagcctg ggggtagata
     5461 tctcccgtcg taacgagctg ccggacacct taggtttacg catcgatatg gtggtcgatc
     5521 tttggtacgc ccggcaaccg tggaatgggc caagccgtca ggaagtagta gaacgggtta
     5581 ttgcccgtgc catttcactg gttgaagaga cacgtccatt attaccaggc gtgcgcgaag
     5641 ctgttgcgtt atgcaaagaa caaggtttat tggtgggact ggcctccgcg tcaccactac
     5701 atatgctgga aaaagtgttg accatgtttg acttacgcga cagtttcgat gccctcgcct
     5761 cggctgaaaa actgccttac agcaagccgc atccgcaagt atatctcgac tgcgcagcaa
     5821 aactgggcgt tgacccactg acctgcgtgg cgctggaaga ctcggtaaat ggcatgatcg
     5881 cttctaaagc agcccgcatg cgttccatcg tcgttcctgc gccagaagcg caaaatgatc
     5941 cacgttttgt attagcaaac gtcaaactct catcgctgac agaactcacc gcaaaagacc
     6001 ttctcggtta atgaccaggg gcagtgatca cgctgcccct ggttctttat ctgaatttgc
     6061 attcaactga cggattaatc gtcaatttaa gagaaagagt tacaccgtca ccacttccgt
     6121 gcactgtata aaaatcctat actgtacgta tcgacagttt agtgagtttt atcatgacgg
     6181 cggaaggtca ccttctcttt tctattgctt gtgcggtatt tgccaaaaat gccgagctga
     6241 cgcccgtgct ggcacagggt gactggtggc atattgtccc ttccgcaatc ctgacgtgtt
     6301 tgttaccgga catcgatcac ccaaagtcgt ttcttgggca gcgattaaaa tggatatcaa
     6361 aaccgatcgc ccgcgctttt gggcatcgtg gttttaccca cagtctgctg gcggtatttg
     6421 cgctgctggc aaccttttac cttaaggttc cggaaggctg gttcattccg gctgatgcgc
     6481 tacaaggaat ggtgctgggt tatttgagcc acatacttgc cgatatgctg acacccgccg
     6541 gtgttcccct gctctggcca tgccgctggc gtttccgctt gcctatcctg gttccccaaa
     6601 agggcaacca actggaacgt tttatctgca tggcattatt tgtctggtcg gtatggatgc
     6661 cccattcatt acccgagaac agcgctgttc gttggtcatc gcaaatgatc aataccttgc
     6721 agatccagtt tcatcggctt attaagcatc aggttgaata ctaaaaaggc aaaaatcacc
     6781 tttctggaat aagcaattcc atttgaatat aagagccagc tcacagttct gttaatcttg
     6841 cgccaacact atgactgcta cgcagtgata gaaataataa gatcaggaga acggggatga
     6901 actttccatt aattgcgaac atcgtggtgt tcgttgtact gctgtttgcg ctggctcaga
     6961 cccgccataa acagtggagt ctggcgaaaa aagtgctggt gggtctggtg atgggtgtgg
     7021 tttttggcct tgccctgcat accatttatg gttctgacag ccaggtactt aaagattctg
     7081 tacagtggtt taacatcgtt ggtaacggct atgttcaact gctgcaaatg atcgttatgc
     7141 cgttagtctt cgcctctatt ctgagcgcgg ttgcccgtct gcataacgca tctcagttag
     7201 gcaaaatcag ttttctgacc atcggtacgc ttttgtttac cacgctgatt gcggcgctgg
     7261 tcggtgtgct ggtcaccaac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     7321 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatcaga
     7381 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     7441 ccaatccgac gtcaatcatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     7501 tgaaactgct gaaggatgat gcgccgaaag gggaacgcgt cttaaccgct atcgataccc
     7561 tacaaagctg ggtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7621 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7681 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7741 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7801 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7861 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7921 gtcagaacgg ctgcgccggt ttgtatccgg caatgctgcg gtgatggttg cgcctacggt
     7981 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     8041 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     8101 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     8161 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     8221 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     8281 ccgttaattc ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta
     8341 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa
     8401 tgtccaactt atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa
     8461 cctcataccc gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca
     8521 caccacttcc cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg
     8581 atattatcga aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc
     8641 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8701 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8761 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
     8821 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8881 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8941 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     9001 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     9061 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     9121 ttattatctt atttaaatct aagtcattta caatataagt ttaagagcga cgccacagga
     9181 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     9241 taattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     9301 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     9361 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     9421 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     9481 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9541 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9601 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9661 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9721 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9781 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     9841 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9901 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9961 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
    10021 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
    10081 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
    10141 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
    10201 acgtattgtt catgcacgcg gctcagcagc tcacggttat ttccagccat ataaaagctt
    10261 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
    10321 taggttctct accgttcagg gtggagctgg ctctgccgat accgtgcgtg atatccgtgg
    10381 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
    10441 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
    10501 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10561 tctgaaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10621 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10681 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10741 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10801 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10861 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10921 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10981 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    11041 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    11101 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    11161 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    11221 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    11281 ataccaagag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    11341 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    11401 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    11461 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    11521 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11581 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
    11641 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccatgaa
    11701 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11761 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11821 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11881 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11941 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    12001 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    12061 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    12121 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    12181 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    12241 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    12301 ggatcacccc gatggcttgc gtcatccagc acttgcagga acagtgcctc gctaatctct
    12361 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattaaccgg taaatcacca
    12421 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    12481 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12541 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12601 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12661 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12721 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12781 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12841 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12901 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12961 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    13021 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    13081 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    13141 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgtgcccca gcttacaggt catttctacc
    13201 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    13261 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    13321 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    13381 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    13441 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    13501 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13561 ccagatactt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13621 ccatttacca gcacatcctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13681 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13741 atattacaca cgccgataaa acgtttaaat ccggtatgac gataaacggt ttcagtaacc
    13801 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13861 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13921 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13981 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    14041 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    14101 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    14161 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    14221 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    14281 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    14341 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgatatc
    14401 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    14461 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgtttttttg
    14521 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
    14581 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
    14641 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactacggta
    14701 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
    14761 ccatctcaac aaactcttcc agccctgttt cgcggaaatt taatgaagaa atcactgttt
    14821 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcaaca aagcaataag
    14881 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc
    14941 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
    15001 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
    15061 ctagagtaaa ttcataatag tcgtgctgat gcagtccgct gatactctca gttttgttat
    15121 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
    15181 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
    15241 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
    15301 tggaccagca ccagactcac tttcatctta ccttcgcccg catcgccttc aatcagtttc
    15361 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggctttcgct
    15421 gcggcaaaat cgccctgctt cgcctgtttc aatgcggcat acgccaggct acgcgcttgt
    15481 ccagagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
    15541 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcaagatta
    15601 cccgtaccgg catcgattaa aatttcaggg gttagcgata tcttcttcgc tctcttcttt
    15661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaacgttgc
    15721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
    15781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
    15841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
    15901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
    15961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
    16021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
    16081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
    16141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
    16201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
    16261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
    16321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
    16381 ggaaagaata ataaagccgg gaattaatgc cgagaaagaa cgcgatactg aagcaggtac
    16441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
    16501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
    16561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
    16621 cccagccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
    16681 tgccattaaa gacattattc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
    16741 accattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
    16801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
    16861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
    16921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
    16981 atacccttaa aaaccgcaat ttaaatatta cggtattgat ttatgaaata actctttgac
    17041 gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa ccgcagcctt
    17101 aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt caaccggttt
    17161 gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc ctaataacac
    17221 gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt caataatgac
    17281 cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag agatagacat
    17341 gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct catcgacgat
    17401 tatctgtcag ccagacactc cgcaagcctt gacctgcttc catgctctgg gtaacttgcg
    17461 aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct gagcaatagt
    17521 atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct cttcacattt
    17581 cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg gcgcaatggc
    17641 cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc tgtggatcag
    17701 tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta tcatctaacg
    17761 cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc tggcaagtac
    17821 cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt gcaacctgcg
    17881 cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg tctttggtac
    17941 gatcataagc tgtacaaccc gccagcatgg ttaataccgc cgctgcactc agaattcctg
    18001 ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa gcctggaata
    18061 cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg attcctgtat
    18121 gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct
    18181 ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac aacaaataat
    18241 aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga
    18301 ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc tcgggatcag
    18361 cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta atgagctgcg
    18421 ccaggaaact cgcaacgaat cactgcaatt tattgccgta cgcctgccct atggtgttca
    18481 ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc gcgtattaac
    18541 cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag caggcattga
    18601 actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag
    18661 cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag aagccattac
    18721 cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt atcgtctcaa
    18781 caaacgccag ggtaaacagt tactgacggc attaggttgc ccggaacacc tttataagaa
    18841 agccccaact gccgatcttg aagatgatcg cccttctctg ccagatgaag tggcactcgg
    18901 cgtgacctat gacaatatcg acgactatct ggaagggaaa aacctacctg aacaggtcgc
    18961 cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc caattaacgt
    19021 tttcgatgat ttctggaaaa agtaataatt tgcacctatt ggattgtgcg aaaaagagta
    19081 atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact atgcaaattc
    19141 aatacattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg tgtaaccttt
    19201 gtcatcaacc cgcctctttt ttaatcgcct cccgcctgtt acactggata gataaccagc
    19261 attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg
    19321 caatttatga atatcccgaa catttacatt cattccttaa tgacttaccc acccgaccag
    19381 gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta
    19441 atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc atgctacggc
    19501 aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg ctccttgaag
    19561 cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc aatcgccagc
    19621 tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt
    19681 tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat
    19741 tgcagagcat cgctgatgaa caaaaacttt gttatggcct gttgggactg gaaccgttaa
    19801 gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca tgctgcggta
    19861 aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt ttgcgagtgg
    19921 tgtgttggcc ttggcaaggt gcggtggcac tgaaagaaca gcacccggaa atgactcaat
    19981 atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa
    20041 cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt tgtaagccgc
    20101 tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag cgagccagtt
    20161 gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc
    20221 gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc acgccccgtt
    20281 aatcacaaac accaccccgc cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct
    20341 gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg acatctgccc
    20401 tgccgtcagt ttcgctttta ccacctggtc cgccgtaaag gcaaaaggct gtaacggttt
    20461 taaggtatgg ttaaagcggt ctgcgctttc aaggagcatc tcgccgcctt ccagcaacgt
    20521 cactatcctt tccatgccgg gaaacaaaga aaactcccca ttcgccgcga tggatgcaat
    20581 gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc
    20641 accggcagcg tttcgccaca gattcaccga cattttacgc atatcaaagt attccatact
    20701 cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatga ttatcggcaa
    20761 ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga gaagagaaaa
    20821 gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac cggcggtctt
    20881 aagttttttg gctgaaagat tattcagcag ttgcaggcat tttacctttt gccgctggac
    20941 gttctgtcag acgcttctca aaattagcat taaattgctt tttctgttcc ggcgtcagga
    21001 tgttgtaaat tttgttctgg gtttccatgt gcgccagcat gttagctttg cgctgttctt
    21061 ccatttttgc gatctgcgct tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt
    21121 catgcattgc gcggcgttct tccagcggcg gacgtttcat ctggtcacgc tggcctttca
    21181 tgatttcgtg gatctgctgt ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt
    21241 cctgatgcgg accgaacttg cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg
    21301 cggtagtggt gtctgcggca tgggccaggt tagccgcgcc aagagccagg gtagaggcaa
    21361 caaacagtgc agttaattta cgcatattct atatccttcc tttcagttat ttattacggc
    21421 tttcttaagt agcgtgccgt gttgacgaga ttaactttac tgactttagc gtcaattaat
    21481 cagagcaacg gtaaaacaat gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa
    21541 aaagaaaatt tgatggagag tgatgagaga atattacaac acgatgattt tgcagagatt
    21601 atgaagaact ataccggatg actggtgata aataaagcaa ataaccagga ttaatctgta
    21661 ttaatttata agaaagcaac ttaatacccg cagaatgatt tctgcgggta agtattagct
    21721 tattttttcg agcattaatc ccgcgcgtaa tcccaacgct accaacggat tagggaataa
    21781 cacatactct acatcatggg ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt
    21841 tcctttctca aacggcataa aattcagcgt gtcacttgcc atatgcattt cgaaggacgg
    21901 cgagtggcga gtaatttgcg aaaccacccg ataacggagc ggcggtgttc tcacgatacc
    21961 gacactctca ccagatagca gcgcagcaat tgcgctggca gttactgcaa actggcgaag
    22021 atcgttttgc ccaaagggca acgctttgcc aagttccaac gtacagacca gcgcgccaaa
    22081 atgtctgacg ctgaaatggg taaacgtacc accaggctcc tgatggaaca ccagcgcctc
    22141 cagccccgcc gcacccagcc acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg
    22201 taatacaccg aactgcggat gcaaggagcc acgaattgcg gtatgtagat caaggtgcca
    22261 acgcacagat tctttgccct ggtcataaaa atcttccagg cactgttcca gttcgcgcgc
    22321 ccgacaggtt tctccgcttt cagcaaatag ctgccaacga ccgccaaaca ttcggttcat
    22381 atcgctatgg caataacgtt tcccttgctt cagcgcagga ggattcccga ggatcaccag
    22441 caaccgccaa cgtaacggga tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat
    22501 ttccacaggt gccgtctcat taccgtggat ccccgcagaa atcaccagcg cgccttgcgg
    22561 tggcgttaat ggcgtcagtt ccagcacacc atcgcccagc cagcgccagc gaacgccgtt
    22621 gatttctcgc tcggtgataa ccggtttttt acccgttaag gtcagagcaa gaaaattatc
    22681 cattgccccc tccctcgcgc tggaacggat aaaccgaacc gagattcagt aattggctca
    22741 atacatccag tgcttcccgc ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag
    22801 taagacgatc gcggtagtaa cgatccaccc agtcattgag cacattaaac aaggtatcgt
    22861 tcatcatcac cgccggattc accgcccggc gttcttcttc tgtcaatacc ccccgcaacc
    22921 gcaggcacgc cgggccgccg ccattcgcca tgctttcacg taagtcaaag acttttagtt
    22981 cactaatcgg gttgtcagcg gcaaggagtt cattgagata accccatact ccggcgtgct
    23041 cccgacactc ctgaggcagc accaacatca tggaaccatc atcgcggctc aacagttggc
    23101 tgttaaacag ataggtagac accgcatccg acacggaaac ctgagttgcc ggaacttcta
    23161 tcgccataaa accgttcacc cgcgtgccag taactgtgac tggcgagcga acgcctgttg
    23221 gtggcaaaac agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgctctg
    23281 gtcgataacg tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattaag
    23341 ccttgccacc gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt
    23401 atcattgcct tcttctcgcc cgtagacaaa aagtagcata cccggttcac cgtaatgacc
    23461 gccgagacga ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc
    23521 cgaatggacg ctaaatttct cttcgtcctt aaaaatcgct tttaacagcg attcagtgac
    23581 gggcgcttcc agcgaacggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt
    23641 gccatccagc gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga
    23701 agcggaactg acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg
    23761 ctcatcgcta ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg
    23821 cgggatcacg gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg
    23881 ttttgccgcc agtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc
    23941 aaacgacagg cccgcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca
    24001 ggcgttcatc gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt ggcgggcaat
    24061 gttaacgagt ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag
    24121 gcgctggggc gatggttacc ggaagcacca atgccgccga atggggcggt actggcagca
    24181 ccagtaagcg gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg
    24241 aatttttccc gctcggggga aaccagaccg caaaagagtc cgaagcgagt gttgttcgcc
    24301 atccgaatcg cctcatcaaa agtatcataa cgccagacgc gcaaaagcgg cccgaacacc
    24361 tcttcatccg gcacaccgcc aacgcccgtc atttcaatga tccctggtgt cagcaacgat
    24421 gtccctgctt gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc
    24481 tgccaggcag taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc
    24541 tgcggttcgt catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagactagca
    24601 agaaacgcat cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt
    24661 tgcccgactg tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc
    24721 acctcatcga taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc
    24781 tgaccggaga gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa
    24841 ccgtcgagat cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg
    24901 ttcagcacgc ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc
    24961 cacggtgtca gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg
    25021 atatgtccgt tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg
    25081 tgcgggcgat gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta
    25141 cgaacgtgat acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt
    25201 gccgcttccc agcgcggctt acccgtttct ctggcaataa tcgcggttaa ttcggcttta
    25261 ttgctttcca gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc
    25321 cgcgcccagc gcggaaacgc cgcacgggct gcccgacaag cctgttcgac ctgagcggca
    25381 tcggcatcat tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat
    25441 gcgccctggc ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg
    25501 cgcacaggcg caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg
    25561 cggcggttaa aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat
    25621 ggtgataatt ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt
    25681 ctgccacttc caccagccga cttttacgga tggcgcgcac gcggtcgatg tcacactcaa
    25741 gcgttggccc gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca
    25801 gcaccgcgcg ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct
    25861 gggataaaaa gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt
    25921 gcccggtgcc gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga
    25981 ggctttgcca gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca
    26041 ctttgtcatt aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca
    26101 aatagccgtt gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc
    26161 tgccggtgtg atcgttacta agaaacagcg tcggcaatgc gttatagaca ttcagctctt
    26221 ttgaggcgtg aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg
    26281 ccacctcaat agcgcaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca
    26341 catagccctg ctcgcttttg ggcagttcac cttgccaggt tttgattgtc ctttcgatac
    26401 gcgccgaaag cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg
    26461 caagctgcat cagcgccgag acatctgagc gctcaacggg acggatgacc atcatgatga
    26521 acctcggcta acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc
    26581 ttcttcgctg acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac
    26641 catcacgcct gctttcgccg cttccagaga gatctgtttc gcttgcccgg cgtaatcggc
    26701 attcagtaca cagccaatca acaaacctaa gccgcgaact tcactgaaca aaccatagcg
    26761 gtgattaata gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag
    26821 catctctggt gtgttgatga gctccagcac tttgcctgct accgccgagg ccagcggatt
    26881 accgccatag gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc
    26941 caacaacgca ccgaccggga aaccgccgcc cagcgctttg gcggtggtta acagatcggg
    27001 cgtcacgccg tagtgcatat aggcatacag ctcaccagtg cgcccgacgc cggtttgtac
    27061 ctcatcaaaa atcagcaagg cattgtggcg gtcacacaat tcacgcagac cttgtaaaaa
    27121 cgcgttgctg gctggcacca caccgccttc gccctagatg ggctcgacaa tcaccgcaca
    27181 ggtggcgtcg tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat
    27241 atccgccggc agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt
    27301 aaacagcgtg cgaccatgaa acgcattttt gaacgccacg atgccactct tatggctgcc
    27361 gtagcggtcg tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc
    27421 ggagttacaa aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg
    27481 cagtaccggc tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt
    27541 cagcgcttca cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc
    27601 gaagtcgata tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg
    27661 tataaagggt gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat
    27721 tggctgagac atagcgacct ctacagtaaa taattcgtta tttgcatgtt aacaataagt
    27781 aatgtttgcg ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata
    27841 aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc
    27901 atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat
    27961 gcagaagtaa aatataattc tagaattgtg atcattgacg aaatttactg gaaattactg
    28021 cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatca ttgacatcat
    28081 taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc
    28141 atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat
    28201 caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga
    28261 tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc
    28321 gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct
    28381 gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca
    28441 gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg
    28501 ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt
    28561 ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat
    28621 tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg
    28681 taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga
    28741 caggctgatg agctgggggg tggtcgatac cttccgccat gctaatccgc aaacagcaga
    28801 tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat
    28861 cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta
    28921 tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg
    28981 ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca
    29041 acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt
    29101 agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg
    29161 agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt
    29221 tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg
    29281 gattgttcgg ttatagcctc tatattctgt tattcatcaa tgccccctct ttctgttacc
    29341 aggaagcata ctggtgatcg ctggaggaat agtttttggc ccgctcttag gacacaactc
    29401 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    29461 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    29521 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    29581 aatattcaaa attacgctta cggattcacc accatcgcct tctggcctta tacccttatt
    29641 tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag cgatctcgcc
    29701 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    29761 attctcatcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    29821 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    29881 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    29941 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    30001 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    30061 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    30121 atcgctgttt atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg
    30181 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    30241 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    30301 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    30361 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    30421 caatgcaaca tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa
    30481 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    30541 aagccaagat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    30601 ctcatcatcc tgttattcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    30661 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    30721 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    30781 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    30841 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    30901 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    30961 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    31021 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    31081 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggca
    31141 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg aactgtttat tctgtttgcc
    31201 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgac
    31261 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    31321 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    31381 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    31441 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    31501 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag
    31561 tggctgactg gcggcaaagc ccgctcttta gcgatgaaga acggctggcg ctggagtacg
    31621 ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc cctgcgtacc cgactggctg
    31681 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    31741 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    31801 aacgttctta aggagggaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    31861 tttctttggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    31921 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    31981 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctc gcggatgccg
    32041 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg tcgtaaaacg ggcggctcgg
    32101 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaa
//
LOCUS       DADLCQ010000037        32125 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000037 DADLCQ010000000
VERSION     DADLCQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.037"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQG95_001560"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JQG95_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY5888156.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JQG95_001561"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JQG95_001561"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY5888157.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JQG95_001562"
     CDS             complement(3736..4092)
                     /locus_tag="JQG95_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY5888158.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JQG95_001563"
     CDS             complement(4096..4512)
                     /locus_tag="JQG95_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY5888159.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JQG95_001564"
     CDS             complement(4623..5336)
                     /locus_tag="JQG95_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY5888160.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="JQG95_001565"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="JQG95_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQG95_001566"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="JQG95_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY5888161.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQG95_001567"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="JQG95_001567"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY5888162.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /locus_tag="JQG95_001568"
     CDS             complement(9023..10438)
                     /locus_tag="JQG95_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY5888163.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="JQG95_001569"
     CDS             10521..11504
                     /locus_tag="JQG95_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY5888164.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQG95_001570"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="JQG95_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY5888165.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQG95_001571"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="JQG95_001571"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY5888166.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14153)
                     /locus_tag="JQG95_001572"
     CDS             complement(13446..14153)
                     /locus_tag="JQG95_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY5888167.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14177..14431)
                     /locus_tag="JQG95_001573"
     CDS             complement(14177..14431)
                     /locus_tag="JQG95_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888168.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="JQG95_001574"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="JQG95_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY5888169.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="JQG95_001575"
     CDS             complement(15306..15515)
                     /locus_tag="JQG95_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY5888170.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..17010)
                     /gene="dinF"
                     /locus_tag="JQG95_001576"
     CDS             complement(15631..17010)
                     /gene="dinF"
                     /locus_tag="JQG95_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY5888171.1"
                     /translation="MPLGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQG95_001577"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="JQG95_001577"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY5888172.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /locus_tag="JQG95_001578"
     CDS             complement(17747..18115)
                     /locus_tag="JQG95_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY5888173.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="JQG95_001579"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="JQG95_001579"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY5888174.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQG95_001580"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="JQG95_001580"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY5888175.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQG95_001581"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="JQG95_001581"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY5888176.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="JQG95_001582"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="JQG95_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQG95_001583"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="JQG95_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY5888177.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELPLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSAERLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQG95_001584"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="JQG95_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY5888178.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQG95_001585"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="JQG95_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY5888179.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="JQG95_001586"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="JQG95_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY5888180.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="JQG95_001587"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="JQG95_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY5888181.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYVWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="JQG95_001588"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="JQG95_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY5888182.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8173 a         8246 c         7781 g         7925 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gttgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaatccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcaaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttaaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa taatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gacaaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaacagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca ggggctggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 taataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga ttaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagtggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgct
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg ggcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctctctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgtctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gcctt
//
LOCUS       DADLCQ010000038        31766 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000038 DADLCQ010000000
VERSION     DADLCQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.038"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQG95_001589"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="JQG95_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY5888183.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /locus_tag="JQG95_001590"
     CDS             1794..2540
                     /locus_tag="JQG95_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY5888184.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="JQG95_001591"
     CDS             2630..3391
                     /locus_tag="JQG95_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY5888185.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="JQG95_001592"
     CDS             complement(3442..4326)
                     /locus_tag="JQG95_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY5888186.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQG95_001593"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="JQG95_001593"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY5888187.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="JQG95_001594"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="JQG95_001594"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY5888188.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="JQG95_001595"
     CDS             6202..6474
                     /locus_tag="JQG95_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY5888189.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="JQG95_001596"
     CDS             6844..7920
                     /locus_tag="JQG95_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5888190.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDMVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="JQG95_001597"
     CDS             7947..9326
                     /locus_tag="JQG95_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888191.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="JQG95_001598"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="JQG95_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="JQG95_001599"
     CDS             10409..11245
                     /locus_tag="JQG95_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY5888192.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="JQG95_001600"
     CDS             11250..12197
                     /locus_tag="JQG95_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY5888193.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="JQG95_001601"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="JQG95_001601"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY5888194.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="JQG95_001602"
     CDS             13324..14082
                     /locus_tag="JQG95_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5888195.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="JQG95_001603"
     CDS             14200..15558
                     /locus_tag="JQG95_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888196.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JQG95_001604"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="JQG95_001604"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY5888197.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGIEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JQG95_001605"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="JQG95_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY5888198.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JQG95_001606"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="JQG95_001606"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY5888199.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="JQG95_001607"
     CDS             19503..20054
                     /locus_tag="JQG95_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY5888200.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="JQG95_001608"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="JQG95_001608"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY5888201.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDLNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /locus_tag="JQG95_001609"
     CDS             21219..23180
                     /locus_tag="JQG95_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY5888202.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="JQG95_001610"
     CDS             23307..24347
                     /locus_tag="JQG95_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY5888203.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="JQG95_001611"
     CDS             complement(24464..25807)
                     /gene="gdhA"
                     /locus_tag="JQG95_001611"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY5888204.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            26043..26315
                     /locus_tag="JQG95_001612"
     CDS             26043..26315
                     /locus_tag="JQG95_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY5888205.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="JQG95_001613"
     CDS             complement(26281..26688)
                     /gene="nudG"
                     /locus_tag="JQG95_001613"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY5888206.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQVLVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            26775..27395
                     /locus_tag="JQG95_001614"
     CDS             26775..27395
                     /locus_tag="JQG95_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY5888207.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(27404..28711)
                     /locus_tag="JQG95_001615"
                     /pseudo
     CDS             complement(27404..28711)
                     /locus_tag="JQG95_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
     gene            complement(28778..29431)
                     /locus_tag="JQG95_001616"
     CDS             complement(28778..29431)
                     /locus_tag="JQG95_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5888208.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(29431..30075)
                     /locus_tag="JQG95_001617"
     CDS             complement(29431..30075)
                     /locus_tag="JQG95_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5888209.1"
                     /translation="MVLLAILADQSTINSEALINSLTMGLVATFIALLLLLLWLEWGA
                     QRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWMLFILQPAW
                     QRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLTAFAVGFSVSIAQYMPTLWLGAG
                     RFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQGIR"
     gene            complement(30032..30496)
                     /locus_tag="JQG95_001618"
     CDS             complement(30032..30496)
                     /locus_tag="JQG95_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5888210.1"
                     /translation="MAVLSEKRLLQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAML
                     AIVAWSLSVVDVAIILGPGNPPTLAVISWQWLTQGDADQQTKGALASLLLMLLLAAYV
                     LAQLAAHYSPRRWHSQACHTFITRHYAGEFFTLNRCAVCGSAGDPRGSVDDQ"
     gene            complement(30526..30948)
                     /locus_tag="JQG95_001619"
     CDS             complement(30526..30948)
                     /locus_tag="JQG95_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888211.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTRWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGSVWASPLQ"
     gene            complement(30921..31766)
                     /locus_tag="JQG95_001620"
                     /pseudo
     CDS             complement(30921..31766)
                     /locus_tag="JQG95_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         7958 a         7603 c         8239 g         7966 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcaaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgccgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc taggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa cctccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatctg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgattgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atatggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gcttcgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatggggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcagca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctatgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccactgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatctg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg cgatattttg
    25681 gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct tgcgcgaact
    25741 cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga gaatatgtct
    25801 gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg tttatgtcat
    25861 gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag aaaaaaatga
    25921 cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata actcagacgc
    25981 taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt aaggagttcc
    26041 ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca ctggctaacc
    26101 cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt tcaggcgggc
    26161 agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat tctgaagggg
    26221 cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa acccttagca
    26281 ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg gaatgtcagc
    26341 aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg cctgatgttc
    26401 atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat ggataatccg
    26461 gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt cgatgtccaa
    26521 ttcttcgttt aactcacgca ccagcacctg ctgctggctt tcatccagct cgactttacc
    26581 tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat
    26641 tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa attacacacg
    26701 ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa aggctttata
    26761 tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg ttgttgcatc
    26821 agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga tggtttaaca ttagtgggat
    26881 ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg gcggcgctag
    26941 tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag
    27001 ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt tacgcgctgg
    27061 tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt
    27121 tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg gcagcaaaac
    27181 atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg ggattaaccg
    27241 aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca tggtttgcgt
    27301 ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg gtgtggagtg
    27361 ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg ctactgcccg
    27421 ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg taccagccgc
    27481 cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag gtttcggacg
    27541 cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata ttccatgctt
    27601 tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta tagaaatctt
    27661 ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt tcaccttttg
    27721 gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa cgaatgctca
    27781 ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg agcatcaact
    27841 gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc ggtggcgttc
    27901 ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc agcagacgca
    27961 catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg tatacgtcac
    28021 gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc atcgctttta
    28081 gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg gtatcgatgt
    28141 agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag gccgcttcaa
    28201 tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc aggtcatgca
    28261 gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg gaaggttcgc
    28321 ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt cgcgttttga
    28381 cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct ttcaggttat
    28441 gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag ctagcagaga
    28501 ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc cagccgttat
    28561 aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt tggtcaagtg
    28621 taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct aaagccattg
    28681 ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta gttttgagtg
    28741 cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg ttgtaatttt
    28801 ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg agatcgtgcg
    28861 ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac tggcgaaaat
    28921 tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac gcttttggct
    28981 gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac aaagtggcag
    29041 gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc acggcattac
    29101 gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc ccgacgctga
    29161 actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt gcggtgggta
    29221 gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac tgtccggcca
    29281 gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc cccattaacg
    29341 tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg ctttctggta
    29401 aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga cataacctac
    29461 ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca gttgccataa
    29521 cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg cctcagtggt
    29581 gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg caatactgac
    29641 tgaaaatccc accgcgaagg cagtcagcac agggcgcaac atgagtgggc atttcacgta
    29701 aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta accgtgaatc
    29761 aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc acagcagatg
    29821 cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca gcgtgtactg
    29881 gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca actggcgacg
    29941 ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg ttgctaccag
    30001 ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat ccgcgaggat
    30061 cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca gcgtagtgcc
    30121 tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag tgcgccgcca
    30181 gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg agtgcgcctt
    30241 tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact gccagcgtcg
    30301 gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac caggcaacaa
    30361 tcgccagcat cgccattgcc agcgaaggcg cgacggaggg caacagcagc cagttcaggc
    30421 attgccagcg gctgtagccc agcgaatcca gcacaatgac ctgctgcaac agccgttttt
    30481 cgctcaatac tgccactaag atccacagca gaaatgcgct ttctttcact gcaagggtga
    30541 ggcccagacc gatccaaatc ggtccattgg cggagtgaaa tacgggaaat agtcatatag
    30601 cagccctccg tcagcaaaga gcagcagggc gctggtggca aaagccacat ggggaatggc
    30661 gagcagccac ggcagacggg cgcacatacg ctgccatttc ggcccaggcc atagcgccac
    30721 aatcaccagc agggcaatca acaatgcccc gaccgccgcg atggttgtcg acaccagcgt
    30781 tgccagtaat gcctgcggta actgtggatc ggcaaataac gcctgccagc gtgtcaacga
    30841 caacgcaggc gatatcagcg tgagagctgc cgggatcagc ggtgcataaa tcaccgccac
    30901 catcgcccac agcaaaaaga ttaatgcgta ccgtaacggc gtaaccattc ttgttccagt
    30961 gcattcaccc atcctgcgtg cggttcagcc agtaccggcg gcagatcctg cggcattctt
    31021 gattgcaata tttcgcgctg cccgtcaggc agtttttgcg gatcgagaac agaaggatcg
    31081 ccccagacaa cgggatctgc tttacgcagt tgcgcatcgg gtgagagcag gaaattggca
    31141 actaccttcg ccgcagcact ggcattcgcg ttggctggag tggtgacgaa atgcacgttg
    31201 ccaatcatcc cctcgcgaaa gccaaaactg taactgcttg ccggcaaatc gccgctggcg
    31261 attttttgct gcgcatgtgc ggggttaaag gtcagcgaca ggcgcaatgt gccggctttc
    31321 agcagagcat ccatccgcgc gggcgaaggc gggaaatcct ttccttcgcg ccacaaatac
    31381 ggatgcagtg catcaagata ttgctacaag ggcgcagtga cacgggcgaa agtcgcatcg
    31441 tccggcgctt cttttaatgc ggcgggatct ggcgtcagca taatcagcaa ctgttcaaga
    31501 aacgccgtgc cggtaaagtc cggtgggcgc ggataagtaa ccgtgccggg attggcttta
    31561 gcaaactcca gtaaggcttg cggcgtttgt ggtggctgtg gcgtaacatc gcggcgggcg
    31621 ataaacgtca gttgtgcgcc gccccagggc gattccgcac cttgtgtcgg cactgaaaaa
    31681 tctccccgca ccggcagctg tgtgtcgaca tagcgccagt tgggcagagt ctccgcccag
    31741 cccgtttgta ataaattggc ctcttt
//
LOCUS       DADLCQ010000039        31724 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000039 DADLCQ010000000
VERSION     DADLCQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.039"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            27..323
                     /locus_tag="JQG95_001621"
     CDS             27..323
                     /locus_tag="JQG95_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888212.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            433..1254
                     /locus_tag="JQG95_001622"
     CDS             433..1254
                     /locus_tag="JQG95_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY5888213.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1550..2197)
                     /locus_tag="JQG95_001623"
     CDS             complement(1550..2197)
                     /locus_tag="JQG95_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY5888214.1"
                     /translation="MILKINQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAV
                     GAYNAGFRKTERQNQRRLAYASEVYRIYTGIKSSKGIRIPVTKKSLSQINSVQNN"
     gene            2473..2856
                     /gene="traM"
                     /locus_tag="JQG95_001624"
     CDS             2473..2856
                     /gene="traM"
                     /locus_tag="JQG95_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAY5888215.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            3048..3695
                     /locus_tag="JQG95_001625"
     CDS             3048..3695
                     /locus_tag="JQG95_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HAY5888216.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFSCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            3803..4042
                     /gene="traY"
                     /locus_tag="JQG95_001626"
     CDS             3803..4042
                     /gene="traY"
                     /locus_tag="JQG95_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAY5888217.1"
                     /translation="MGGRVIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNE
                     ALVRLQDHLQRYPDFYNEEIFREVIEENESTFKEL"
     gene            4076..4438
                     /gene="traA"
                     /locus_tag="JQG95_001627"
     CDS             4076..4438
                     /gene="traA"
                     /locus_tag="JQG95_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAY5888218.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLV
                     GFAILSVFIAVGMSVAGF"
     gene            4453..4764
                     /gene="traL"
                     /locus_tag="JQG95_001628"
     CDS             4453..4764
                     /gene="traL"
                     /locus_tag="JQG95_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAY5888219.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            4786..5352
                     /gene="traE"
                     /locus_tag="JQG95_001629"
     CDS             4786..5352
                     /gene="traE"
                     /locus_tag="JQG95_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAY5888220.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            5339..6067
                     /gene="traK"
                     /locus_tag="JQG95_001630"
     CDS             5339..6067
                     /gene="traK"
                     /locus_tag="JQG95_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAY5888221.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            6067..7518
                     /locus_tag="JQG95_001631"
     CDS             6067..7518
                     /locus_tag="JQG95_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HAY5888222.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGTSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            7487..8074
                     /gene="traP"
                     /locus_tag="JQG95_001632"
     CDS             7487..8074
                     /gene="traP"
                     /locus_tag="JQG95_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAY5888223.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAAD
                     AADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADEHLKE
                     VYTEDTSLSEKIRKCHVYFPDDRANRNNGDKNEHA"
     gene            8013..8381
                     /locus_tag="JQG95_001633"
     CDS             8013..8381
                     /locus_tag="JQG95_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAY5888224.1"
                     /translation="MFISLMTGQTETTEIKMNMRNINIITARSVPDKSVSDDFIHAVL
                     SNCTTRIVLPALEKFSPELLPHNFNMAAVGVMKEGMSMRLFSSPGHIVVTGQSGGGKS
                     VPVKELIDRIIQKYKEPFDE"
     gene            8374..8625
                     /locus_tag="JQG95_001634"
     CDS             8374..8625
                     /locus_tag="JQG95_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HAY5888225.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGEERIVA
                     YVYKSSRNTVFENPDIVKNCTVRDLKNDFVNCDEKGEGQ"
     gene            8622..9137
                     /gene="traV"
                     /locus_tag="JQG95_001635"
     CDS             8622..9137
                     /gene="traV"
                     /locus_tag="JQG95_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAY5888226.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPAAASLPRLADGNFRTTSVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            9272..9493
                     /gene="traR"
                     /locus_tag="JQG95_001636"
     CDS             9272..9493
                     /gene="traR"
                     /locus_tag="JQG95_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAY5888227.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            9486..9899
                     /locus_tag="JQG95_001637"
     CDS             9486..9899
                     /locus_tag="JQG95_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888228.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            9892..10365
                     /locus_tag="JQG95_001638"
     CDS             9892..10365
                     /locus_tag="JQG95_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888229.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            10443..10661
                     /locus_tag="JQG95_001639"
     CDS             10443..10661
                     /locus_tag="JQG95_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888230.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            10689..11036
                     /locus_tag="JQG95_001640"
     CDS             10689..11036
                     /locus_tag="JQG95_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888231.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            11162..13792
                     /gene="traC"
                     /locus_tag="JQG95_001641"
     CDS             11162..13792
                     /gene="traC"
                     /locus_tag="JQG95_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAY5888232.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGMSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            13789..14175
                     /gene="trbI"
                     /locus_tag="JQG95_001642"
     CDS             13789..14175
                     /gene="trbI"
                     /locus_tag="JQG95_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAY5888233.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            14172..14804
                     /gene="traW"
                     /locus_tag="JQG95_001643"
     CDS             14172..14804
                     /gene="traW"
                     /locus_tag="JQG95_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAY5888234.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            14801..15793
                     /gene="traU"
                     /locus_tag="JQG95_001644"
     CDS             14801..15793
                     /gene="traU"
                     /locus_tag="JQG95_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAY5888235.1"
                     /translation="MKRRLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            15823..16128
                     /locus_tag="JQG95_001645"
     CDS             15823..16128
                     /locus_tag="JQG95_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888236.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            16137..16775
                     /gene="trbC"
                     /locus_tag="JQG95_001646"
     CDS             16137..16775
                     /gene="trbC"
                     /locus_tag="JQG95_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAY5888237.1"
                     /translation="MNRKIFVLFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            16772..18622
                     /gene="traN"
                     /locus_tag="JQG95_001647"
     CDS             16772..18622
                     /gene="traN"
                     /locus_tag="JQG95_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAY5888238.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            18649..18906
                     /gene="trbE"
                     /locus_tag="JQG95_001648"
     CDS             18649..18906
                     /gene="trbE"
                     /locus_tag="JQG95_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAY5888239.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAFVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKTDERKNDERNEDE"
     gene            18899..19642
                     /gene="traF"
                     /locus_tag="JQG95_001649"
     CDS             18899..19642
                     /gene="traF"
                     /locus_tag="JQG95_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAY5888240.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKITPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            19656..19997
                     /gene="trbA"
                     /locus_tag="JQG95_001650"
     CDS             19656..19997
                     /gene="trbA"
                     /locus_tag="JQG95_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HAY5888241.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(19978..20313)
                     /locus_tag="JQG95_001651"
     CDS             complement(19978..20313)
                     /locus_tag="JQG95_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HAY5888242.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            20394..20678
                     /gene="traQ"
                     /locus_tag="JQG95_001652"
     CDS             20394..20678
                     /gene="traQ"
                     /locus_tag="JQG95_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAY5888243.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            20665..21210
                     /gene="trbB"
                     /locus_tag="JQG95_001653"
     CDS             20665..21210
                     /gene="trbB"
                     /locus_tag="JQG95_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAY5888244.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            21140..21487
                     /gene="trbJ"
                     /locus_tag="JQG95_001654"
     CDS             21140..21487
                     /gene="trbJ"
                     /locus_tag="JQG95_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HAY5888245.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            21435..21860
                     /gene="trbF"
                     /locus_tag="JQG95_001655"
     CDS             21435..21860
                     /gene="trbF"
                     /locus_tag="JQG95_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAY5888246.1"
                     /translation="MNHRKYQRKLIMKEKRNDAKLKNRKTKRDYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRRENKLREGGNNASH"
     gene            21847..23220
                     /locus_tag="JQG95_001656"
     CDS             21847..23220
                     /locus_tag="JQG95_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HAY5888247.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            23217..26042
                     /gene="traG"
                     /locus_tag="JQG95_001657"
     CDS             23217..26042
                     /gene="traG"
                     /locus_tag="JQG95_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAY5888248.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTTASAGLY
                     GDAHAGLDAKPSLFGVRVDVGAKVGGRASIDGSDLDSHEASSGSRASHDARHDIDARA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSGEAVKKMIDDKKERLKGPL"
     gene            26039..26548
                     /gene="traS"
                     /locus_tag="JQG95_001658"
     CDS             26039..26548
                     /gene="traS"
                     /locus_tag="JQG95_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HAY5888249.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLQADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKIISF
                     MYDKNKTYILSFLIAFSMVSFAHTLLYEFLLIALFIIFFFIYAIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            26562..27293
                     /gene="traT"
                     /locus_tag="JQG95_001659"
     CDS             26562..27293
                     /gene="traT"
                     /locus_tag="JQG95_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAY5888250.1"
                     /translation="MKTKKMMVVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            27496..28233
                     /locus_tag="JQG95_001660"
     CDS             27496..28233
                     /locus_tag="JQG95_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888251.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            28284..30446
                     /gene="traD"
                     /locus_tag="JQG95_001661"
     CDS             28284..30446
                     /gene="traD"
                     /locus_tag="JQG95_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HAY5888252.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGHQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQAEQPQQPQQ
                     PQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYE
                     AWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            30446..>31724
                     /locus_tag="JQG95_001662"
     CDS             30446..>31724
                     /locus_tag="JQG95_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY5888253.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVL"
BASE COUNT         8121 a         7144 c         8557 g         7902 t
ORIGIN      
        1 tgaaaacatc agatacagga ggaattatga gcgcattttc ttttaacacc acttatcagc
       61 caacaggcga tcagcagaaa gacgcaatcg cacaaatcgg gatcatgcag aatcgtgctg
      121 ttcaggcaaa tcttgcatat cagagctgcc gggattctgg tgcactcttt aaggtgctcc
      181 accaggtcaa taatgagctg catgacttac tggatagcct ggagaatcat actcctcttg
      241 tcagaaaaaa cgctgatgag ttgatcgcgc tgttactgct tttcactcgg caggttggtc
      301 agtcccgaac tgacctgatt tgatggtcct tcccctcgga attgttgtct ttttgttaac
      361 gataaccgag ggggatgata ttaagcgaaa aaacttttat taattttatg ccttcataaa
      421 ggagttactg ttatgcgatt agcttcccgt tttggtcgat ataattctat tcgccgtgag
      481 cgcccgttaa cggatgacga attaatgcag tttgttccat ctgtcttttc tggtgataaa
      541 catgaatccc ggagtgagcg ttatacatat atcccaacca tcaatatcat caataagtta
      601 cgtgatgaag gtttccagcc gttctttgcc tgtcagagtc gggttcgtga tttgggacgc
      661 cgcgaataca gtaaacatat gttacgtctt cgcagggaag ggcatattaa cgggaaagaa
      721 gttcctgaaa ttatcctgct taattcacat gatggttcat ccagttatca gatgatcccc
      781 ggaatttttc gttttgtctg cacaaatggc ctggtgtgcg gaaataattt tggcgaaatc
      841 cgtgttccac ataaaggtga tattgtcggg caggttatcg agggagcgta tgaagtgctc
      901 ggtgtctttg ataaggtcac tgataatatg gaggcgatga aagaaattca tcttaacagt
      961 gacgagcaac atttatttgg cagagctgca ctgatggtca ggtatgaaga tgaaaataaa
     1021 acgccagtga cgcctgaaca aataattact ccccgtcgtc gggaagataa acagaacgat
     1081 ctctggacaa cctggcagcg ggttcaggag aatatgataa aaggtggatt atcggggcga
     1141 agtgcctccg ggaaaaatac caggacaaga gccattacag gtattgatgg tgatataaga
     1201 atcaacaagg cgttatgggt gattgccgaa cagttcagaa agtggaagtc atgataatat
     1261 aagtaaagcc ccgaaaattt ttcggggctt tacttatatt atcattcttc ggcatcgctg
     1321 tgtcgtctga tatacctcaa ttccatcaac cagctttccg gttgtctgta aatattgtgc
     1381 ccgcgaaaaa tggtctggca ggaggctgcg cagttaccgg gtatatataa gatgggttcc
     1441 cgctttgcgg ggcttcggcc agtcagcatt atcactcata tgatattttt gtgtgtggcc
     1501 ttccatgccg ctgtcgcggc ataaccggta tttgacaatg tatacaatat taattgttct
     1561 gcacactgtt aatttgggaa agtgatttct tcgtgaccgg aatccggata cctttactgc
     1621 tttttatccc ggtataaatc cggtaaacct ctgatgcgta ggcaagacgt ctctggttct
     1681 ggcgttcggt cttcctgaat ccggcattgt atgcaccaac ggcctcccag gagacgcccc
     1741 attttttaaa ggctattgcc agataataag caccggtata aatgttcatg cagggatctg
     1801 ttgtcagatg ttccggctta attccatagc gggccagttc gttaaaatgc tgggaatcta
     1861 cctgcatcag tccgctgcca tatcccgtta ccggattaat accgatggca ttaacccggt
     1921 aacgggattc tttccatgat atcgctctca gtaaatccgg gtctattttg taatcccgac
     1981 ctgcaagatc aaagcaatcg gcggcttcgc agatcccatt tataaacatc aggcagattg
     2041 ctaacatcca ttttttcatt tttccacctc tggtgacttt atccgtaaat aatttaaccc
     2101 actccacaaa aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg
     2161 caaaaacaag tttttgctga tttatcttta atatcattga gttgtatttg tggatttata
     2221 ttgtttaaat ctgatttatt taaagcagcg tcgttaacgc agctacagca acgcgccgac
     2281 accgctttgt aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt
     2341 gctgctagcg gcgcggtgtg tttttttata ggataccgtc aggggcgctg ctagcggtgc
     2401 gtccctgttt gcactatgaa ttctagtgtt tcgaaattaa ctttgtttta tgtttaaaaa
     2461 aggtaatatc taatggctaa ggtgaatctg tatatcagca atgatgctta tgaaaaaata
     2521 aacgcgatta ttgaaaaacg tcgacaggag ggggcgagag aaaaagatgt cagtttttca
     2581 gcaacggctt caatgcttct tgaactgggt cttcgtgtat atgaggctca gatggagcgc
     2641 aaagagtctg catttaatca ggccgagttt aataaactac ttcttgaatg cgttgtaaaa
     2701 acacagtcat cagtagcgaa aattttgggt attgagtctc tcagtcctca tgtctcagga
     2761 aatccaaagt ttgaatatgc caatatggtt gaagatatca gagagaaggt gtcggctgag
     2821 atggaaaggt tctttccaaa aaatgatgat gaataaaggg aaataaattt tgactttgtt
     2881 caaatatcag actttttaat ggttcaaact gatgacaata atcagaataa ttagtatatt
     2941 aattacgtgg ttaatgccac gttaaaattt gaacttgaaa atcgccgatg cagggagctt
     3001 gtccctccct gcatcgactg tccatagaat cctttgagga ggttcctatg tgtgcgatgg
     3061 atactagaga aagagcactc atacgtcaat caggcctgcc ggaaattatt ttatccgcaa
     3121 aatatccagt ctgtgtccgc acagagtcag gaaaattcat agactcaaac tctgtttttt
     3181 tacacttaat aaagtgtcag aatagtgatt ctgaaatatg gttttctggt attgatattg
     3241 atacgcaaat tttatttcat actgctgaag ttgatgcttt ctcgtcctcg aatggagtga
     3301 gtattattaa tggtgtaata ttgaatgatg tatcatggga tgtggtggtt gaaagtacgg
     3361 aagtgaataa cttgaatttg tttgtctgga gattctttga aagatacgta gttagtggct
     3421 tttttataaa gggcaaggag tttagttgtg aacctgacaa atatcgcttt aataagataa
     3481 taccgtatct tttgggttat agtcatgagt attcttctca gagaatggat atctctgttg
     3541 accaatctaa aaaaatattt atgaatttta aaaaacacta tggtttctca gacagggatg
     3601 agtggttgcg atatgctata atctctgatg tgttatttta tctatatcaa tacttcgcta
     3661 gtaatattaa cagccttcac tgtaaagaaa tgtaagcatt cccggcgcta aaagtgccgg
     3721 tttttgttaa cgtaatttac tattttgttg tttatttttg tgtgcgtctt atgtgaatat
     3781 tttgactaaa tctcttttat ttatgggagg gagggtgatt aaaggagctt taagatctgc
     3841 gccaagtaga aaagttttac ttacccttga tgaggaaacg aataaattat tacttggagc
     3901 cagagagcgt agtggaagaa cgaaaacaaa cgaagcattg gtgaggttgc aggatcattt
     3961 gcaacgttat ccagattttt ataatgaaga aatattcagg gaggtgatcg aagagaacga
     4021 gtcaacattt aaagaacttt agttttcatc tgtcaatatt aatcagagga atcatatgaa
     4081 tactgtttta agtgttcagg gtgcttctgc gcccgttgaa aagaaatcgt ttttttctaa
     4141 attcactcgt ctgaatatgc ttcgcctggt tcgtgcagtg atcccggttg ctgttctgat
     4201 gatgttgttc cctgagttag ctatggctgc tggtaaaggc acggacctga tggctaaagg
     4261 tcaggaaacg gttaaggcga ccttcggtaa ggactccagt atcgttaaat gggttgttct
     4321 ggctgaggtg ctggtcggtg cggtcatgta catgatgacc aaaaacgtca agttcctggt
     4381 cggctttgcc atactctctg tatttattgc agtaggtatg tctgttgccg gattctgaca
     4441 ggaaataaaa cgatgtcggg agacgagaat aaacttaaga aatatcgttt cccggaaaca
     4501 ctgaccaacc agagccgctg gtttggcctg ccactggatg aactgatccc cgcagcaatc
     4561 tgtattggct ggggtatcat aacatcgaaa tatctgttcg gtattggtgc agcggttctg
     4621 gtttatttcg ggattaaaaa actgaaaaaa gggcggggca gttcctggtt acgtgacctg
     4681 atttactggt atatgccaac agccctgctg cgcggtattt ttcataatgt tcccgattcg
     4741 tgtttccggc agtggattaa atagaactga taccaggatt gttatatgga acacggtgcc
     4801 cgtttaagta ccagtcgtgt aatggccatc gcctttatat ttatgtcagt gcttattgtt
     4861 ctcagcctct ctgttaacct cattcagggg gtgaataact accgtcttca gaatgagcaa
     4921 cgcactgccg tgaccccaat gggatttaat gtgccctttg ccgtgtcaca gaacagtgcc
     4981 gatgcctctt atttgcagca gatggcgctg tcatttattg ccctccgtct gaatgtctca
     5041 ccggaaatcg tcgatgcttc acatcaggcg cttctgcaat atatccgccc gggtgcacag
     5101 aaccagatga aagttattct ggctgaagaa gcgaaactca ttaaaaaaga taacgtgaat
     5161 tctgcttttt tccagaccag tgtccgtgtc tggcctcagt atggtcgtgt ggaaatccgt
     5221 ggaattcgga aaacatggat tggtaactcc gaacctttta ctgatatcaa acattacatc
     5281 cttattctga agcgggaaaa cggggtgacc tggctggata atttcgggga aacagacgat
     5341 gagaaaaaat aatacggcaa taatattcgg cggcctgttt ttttcctgca gcgtgatggc
     5401 cgcaaacggt acgctggccc ccactgtggt gccaatggtg aacggtggtc aggccagtat
     5461 tgccatcagc aataccagcc cgaatctgtt taccgttccc ggtgaccgga ttatcgccgt
     5521 gaacagtctg gatggtgccc tgaccaataa tgagcagacc gcctccggcg gtgtggtggt
     5581 ggccaccgtc aacaaaaagc cctttacgtt cattctggaa acagagcgtg gtctgaacct
     5641 ttccattcag gctgttcccc gtgaaggcgc ggggcgtacc attcagctgg tcagtgacct
     5701 gcgcggaacc ggagaagaag ccggagcgtg ggaaacgtcc acgccttacg aatccctgct
     5761 tgtgaccatc agccaggctg tccgtggcgg aaaattacct gcaggctggt atcaggttcc
     5821 tgtgacaaag gaaaccctgc aggccccggc ggggctgtct tcagtggcag atgccgtatg
     5881 gacggggaat cacctgaaga tggtccgctt tgtcgtggaa aataaaacgc tgtctgccct
     5941 gaatatccgg gaaagtgatt tctggcagcc gggaacccgt gccgtgatgt tcagccagcc
     6001 tgccagccag ttactggcag gtgcgcgcat ggatgtgtat gtcatccgtg acggggaggg
     6061 caactgatgg ccagtatcaa taccattgtg aaacgcaagc agtacctgtg gctggggatt
     6121 gtggttgtcg gtgcagcctc cgcgattggt ggggtactgt atctgtctga tgtggacatg
     6181 tccggtaacg gtgaggcggt ggctgaacag gagcctgtgc cggatatgac aggtgtggtg
     6241 gatacgacct ttgatgacaa ggtgcgtcaa catgccacca cagagatgca ggtgacggca
     6301 gcgcagatgc agaagcagta tgaggaaatc cgtcgtgaac tggatgtcct gaacaaacag
     6361 cgcggtgatg accagcgtcg tattgaaaag ctgggacagg acaatgccgc cctggcagag
     6421 caggtaaaag ccctgggtgc taatcccgtc acggcgaccg gtgagcctgt acctcagatg
     6481 cctgcctcac cgcccgggcc ggaaggcgaa ccacagccag gaaacacccc cgtatccttc
     6541 ccgccgcagg gcagcgttgc tgttccacct ccgacggcgt tttatcccgg gaatggtgtc
     6601 acgccaccac cacaggtgac gtaccagtct gtgccggtgc ctaaccggat acagcgtaag
     6661 gtgtttaccc gtaatgaggg aaaacaggga acatcgctgc cgtacattcc gtcaggaagt
     6721 tttgcgaaag ccatgctgat tgaaggggcg gatgccaatg cctcagtcac cggtaatgaa
     6781 tccacggtgc cgatgcagtt gcgtatcacc ggcctggtgg aaatgccgaa cagcaagacg
     6841 tatgacgcaa cgggatgttt tgtggggctg gaagcctggg gggatgtgtc cagtgagcgt
     6901 gccattgtac gcacccgcaa tatcagttgc ctgaaggacg gcaaaactat tgatatgccg
     6961 attaaggggc atgtcagctt ccggggtaaa aacggtatca agggcgaagt ggtgatgcgt
     7021 aacggcaaaa tcctcggctg ggcatggggc gcgggatttg ttgacggtat cggtcaggga
     7081 atggagcgcg cctcccagcc ggctgtcggg ctgggtgcca cagccgctta cggggctggt
     7141 gatgtcctga aaatgggtat cggtggcggc gcatcgaaag ccgcacagac gctcagtgac
     7201 tactacatca aacgtgccga acagtatcac ccggtgatac cgattggtgc gggcaacgaa
     7261 gtgaccgtgg tgttccagga cggcttccag ctgaaaaccg tggaggaaat ggcgctggag
     7321 cagtcacaga gtacagcgga agagggtaac ccggaaagcc cggtgccgaa tcctccctcg
     7381 gcagaaagcc acctgaacgg ttttaacacc gaccagatgc tgaaacagct gggcaatctg
     7441 aatccgcagc agtttatgtc cggagacaag aacccgctgg gcggggatgc cggcaaggag
     7501 ggcggaaatg gcgggtgatg cactgacatt ccgtgtggcg tttgtcgttc gctggctgct
     7561 gtgggtcgtc aggtttgtgg ttatctggcc gctggccaca atggccctga tggctctgtt
     7621 tgttttatgg aaggacaaca ccacgccggg gaaattactg gttaaggaga ttaacttcgt
     7681 aagacaaaca gcacctgccg gacagttccc ggtcagtgaa tgctggtttt cttcctctga
     7741 ttcttcaggc agatcagaaa tacaggatat ctgccattac cgcgcggctg atgctgcaga
     7801 ttatgtcagg gagactgacc ggtcactgat gcaactggtc accgcattct gggcaacgct
     7861 ggcactgatg tatatgtcgt ttgcggcagg caccgggaaa tatccgatgt ggcccggaaa
     7921 aatgaaatgt gtccgggtgg gaaccgcaga tgaacatctg aaggaagttt atacggaaga
     7981 tacttctcta tcggaaaaaa tcaggaaatg ccatgtttat ttccctgatg acagggcaaa
     8041 cagaaacaac ggagataaaa atgaacatgc gtaatattaa tattattact gcacgctctg
     8101 taccggataa aagcgtatca gatgatttta tacatgctgt tctcagtaac tgcaccacaa
     8161 ggatagttct tcctgctctg gagaaattca gccctgaatt attgccgcac aattttaata
     8221 tggctgctgt cggtgtgatg aaggagggaa tgtcgatgcg tttattttct tctccggggc
     8281 atattgtggt taccggacaa tccggtggcg gaaaatctgt tccggtgaaa gaactaatcg
     8341 acagaattat tcagaaatat aaggaaccct tcgatgaata agctggtttc tgatggcagc
     8401 gtcaaaaaaa taaattatcc ggtgctgtat gagtccggaa taacacctcc gttatgtgaa
     8461 gtcagtgcac cagaacctga tgcgggggaa gaacgcattg tggcatatgt ttataaatca
     8521 tcacggaata cggtttttga aaatcctgac attgtaaaaa actgtacggt cagggatttg
     8581 aagaatgatt ttgtgaactg cgatgagaaa ggtgagggac aatgaaacag atttctttat
     8641 ttattccgct gctggggacg ttattacttt ccggctgtgc cggaacgaac tccgattttg
     8701 agtgtgatgc caccacatcc gatacctgta tgactatgga gcaggccaat gagaaggcca
     8761 aaaaactgga gcagtcctca gaagcaaagc cggctgcggc atcactgccg cgcctggctg
     8821 acgggaactt ccggaccacg tcggtgcagg ccgttgcggc gacaacgccg tcccgcagca
     8881 ggcctgctgt gacagcactt ccggagcaga aactgctggc accacgcccg ctgtttaccg
     8941 ctgcccggga agtgaaaacg gttgttccgg tcagttcagt tgcgccggta acacctcccc
     9001 gtccgctaag aacgggtgag cagacggcag cattatggat agcgccttat attgataatc
     9061 aggatgttta tcatcagcca tccagtgtgt tttttgttat taaaccgtct gcgtggggaa
     9121 aaccacgtat taattaactg gccctgaagt tgtatatcag ccggcaatgc cggcgggctt
     9181 tctgtattca aaatctgcct gtaaaacgag tgccctgtgc gtgaaaaggg acgggcggta
     9241 gcgtgtcaga acatgtcggg aggaatattc cgtgagtgat gaagccgatg aagcatattc
     9301 agtgacagaa caactgacca tgacaggaat aaaccggata cgccagaaaa taaatgttca
     9361 tggtattcct gtttatctct gtgaagcatg cggaaatcct attccggaag cccggcggaa
     9421 aatatttccc ggtgtgacgt tgtgcgttga atgtcaggcg tatcaggaaa ggcagaggaa
     9481 acactatgca taaatcagaa aatgaaaaaa tagattttat tctggatgag catgagcgga
     9541 tattcagaaa acaggggtct ttgtgtatgg cactgggtaa gggcttttta gttatagcta
     9601 ttctttgtgg tgttgccaca ttctgtgttt ctggcctgta tctgaaatcc ctgtgcctgt
     9661 ccgttgcctg ctgttgtggg gtattcagtc gtatttttaa ttattacagc gtaccggagg
     9721 aatcacgcat ggtactcagt cgtcaggaat tactccggct tatgcgtctg actgaagacg
     9781 taccagatgt gagacaggaa ctgcttaacc gtttgttgtc cggtaaaaaa ctgactggtc
     9841 gggatgaaca ggatatccgg cgtctctggc aggaaacgca gaggaaacac tatgcataaa
     9901 tcagaagctg aaaaaataga ttctattctg gatgaacatc aacagatata tcgcagacat
     9961 aaccgaatag ataacatcct ggcgtattgt gcgtgtattg catggattcc ctgtattatt
    10021 gctgcgttcg gcgttgatgg tatatatctg aaaatcctgt ttgcggtcct tgcctgctgt
    10081 ggtgcggtat gcttttttat tttcttcttc accctcctgc cggaatcact catggtcctc
    10141 agccgtcaga gcctgcttca gcttatgcgt ctgactgaag acgtaccgga tgcgagacag
    10201 gaactgctta accgtctgct gtccggtaaa aaactgactg gtcgggatga aaaggatatc
    10261 cggcgtctct ggcaggagaa agtggatgca atgcaggagt ctgccacccg tcagcgtgaa
    10321 caggatacta ttcggaagtt tactgaaggg aataaatccg aatgagaatc tgcggtgttt
    10381 ctgacctcat agttaactga atgaatattc agtgattaat atttaactgg aggtaataaa
    10441 gaatgcatac ctttattttt cttgtgctga taaaaaatat taacggtggc ggaagctgga
    10501 atgttacccc catgccaaat acggacgtat gcgtgcaggt tcttcggaca atcgaagcac
    10561 atggcggcag gggctttgga aatacagggg aatgtattga agttaaaaca aatgaacctt
    10621 tgaacagcgt gatggaaaca ctaaatggtg gtgggcaatg agtgcttact gcttcgaagg
    10681 aaaatgaaat gaagtcgata tatttaaaat ctgttctggc ttttatattt gttggcgtta
    10741 tggctatgat tgtatgtatt cctttttaca tcgtttacct tgcgcaacaa cctgcaacac
    10801 cagaacaact gacagagatt ttacaggaaa ctccctgtgc agcagaagca tttcaggaga
    10861 cacttaatta tcagtctgaa cctttaacac ttggtaaggc aaataaaatc gcatcagaat
    10921 gcagaaaaag aaacgaaatg gcagaggtga aacgggtaag agaaaatgag cgcaataaga
    10981 ttcgcgaaaa acaaattcag gcattaaatg atgctcattc agtaaaggaa cgttaatcct
    11041 ggtttcctgt taaaagcctc catctcttat ttgtgctgtg gtttcaggaa tggcactgcg
    11101 tgatatatcc acggccacag actgatattt tcctttttta aacgcactaa ggaatactca
    11161 tatgagtaat aacccacttg aggccgtcac tcaggcggtt aactccctcg tcacagcact
    11221 gaaactgcct gacgaatccg caaaggccaa tgaagttctg ggcgaaatga gcttcccgca
    11281 gttcagccgt ctgctgccgt accgtgatta caaccaggaa tccggtctgt tcatgaatga
    11341 caccacgatg ggctttatgc tggaagccat tcccatcaat ggggcgaata agtccattgt
    11401 ggaggcactc gatcacatgc tgcgcaccaa actgccgcgc ggtattccgt tgtgtatcca
    11461 tctgatgtcc agtcagctgg ttggtgacag gattgagtac gggctgcgtg agttctcctg
    11521 gtcaggtgaa caggctgaac ggtttaacgc cattacccgg gcctattata tgaaagcggc
    11581 agcgacacag tttccgctgc cggaggggat gaatctgccc ctgaccctgc gccattaccg
    11641 ggtatttatc tcgtactgtt ctccctcgaa gaaaaaaagc cgggctgaca ttctggaaat
    11701 ggaaaacctg gtgaaaatca tccgggcgtc gttacagggg gccagtatcg ccacacagac
    11761 ggtggatgca caggccttta tcgatattgt cggggagatg attaaccata acccggactc
    11821 cctgtacccg aaaagacgtc agctggaccc gtattctgat ctgaattatc agtgtgtgga
    11881 ggacagtttt gacctgaatg tccgggctga ttacctgacg ctgggcctgc gtgagaacgg
    11941 caggaacagc acggcccgta tcctgaattt ccatctggcc cgtaacccgg aaatcgcctt
    12001 cctgtggaat atggccgaca actacagcaa cctgctgaac ccggaactgt ccatctcctg
    12061 tccgttcatc ctgacgctga ctctggtggt ggaagaccag gtgaaaaccc acagcgaagc
    12121 caacctgaag tacatggacc tggagaaaaa gtcgaagacc tcctatgcca aatggtttcc
    12181 gtccgtggag aaagaggcga aggagtgggg ggaactgcgt cagcgtctgg gctccggtca
    12241 gtcttctgtc gtgtcctact tcctcaacat cacagcattc tgcaaggaca acaatgaaac
    12301 ggcgctggaa gtggagcagg acatcctgaa cagcttccgt aaaaacggtt ttgagctgat
    12361 ttcaccgcgc tttaaccaca tgcgcaattt cctgacctgt ctgcccttta tggccgggaa
    12421 agggttgttt aaacagctga aagaggccgg agtggttcag cgcgcagaga gctttaatgt
    12481 tgccaacctg atgccgttag tggcggataa ccccctgaca ccggcaggtc tgctggcacc
    12541 cacctaccgt aaccagctgg cgtttatcga tattttcttc cggggaatga ataacaccaa
    12601 ctacaacatg gcggtctgtg gcacctccgg ggccggtaaa accgggctga tacagccact
    12661 tatccgcagc gtgctggact ccgggggctt tgccgtggtg ttcgacatgg gggatggcta
    12721 caagtccctg tgtgagaaca tgggtggcgt atatctggac ggtgaaaccc tgcgttttaa
    12781 cccgtttgcg aacatcaccg atattgacca gtcagcggag cgtgtccgtg accagttgtc
    12841 agtgatggcc agccccaacg gtaacctgga tgaagtgcat gaaggtctgc tgctgcaggc
    12901 ggtcagagcc tcctggctgg ccaaagagaa cagggcacgt attgatgacg tggtggattt
    12961 cctgaaaaac gccagtgaca gcgagcagta tgccgggtca ccgaccatcc gcagccgtct
    13021 ggacgaaatg attgtgctgc ttgaccagta cactgccaac ggcacttacg gccagtattt
    13081 taactccgat gagccgtccc tgcgggatga tgcgaaaatg gtggtgctgg agctgggcgg
    13141 actggaagac cgtccgtcac tgctggttgc ggtgatgttc tccctgatta tctacatcga
    13201 gaacaggatg taccgcacgc cgcgtaacct caagaaactg aacgttattg atgaaggctg
    13261 gcgtctgctg gacttcaaaa atcgtaaagt tggtgagttt attcaaaaag gttaccgcac
    13321 ctgtcgccgt cataccggtg cctatatcac catcacacag aacatcgtcg actttgactc
    13381 tgacaaggca tccagcgcag cccgtgcggc atggggtaac tcctcctaca aaattatcct
    13441 caaacagagt gcgaaggagt tcgcgaaata taaccagtta ttcccggatc agttccagcc
    13501 actgcagcgc gacatgattg gtaagtttgg tgcggccaaa gaccagtggt tcagttcctt
    13561 cctgctgcag gtggaaaacc attcctcctg gcaccgtctg tttgtggacc cgttaagccg
    13621 tgccatgtac agctctgacg gcccggattt tgagtttgtg cagcagaaac gcagagaggg
    13681 tatgagtatt catgaggcgg tgtggcagct ggcgtggaag aaatcggggc cggagatggc
    13741 ttcgctggag gcctggctgg aagaacatga gaaatacagg agtgttgcat gaccacaacg
    13801 caaaaaacga ctgacgttac agccccccga cgcagccact ggtggtggac cgtgccggga
    13861 tgcctggcaa tggtgttact gaacgcagcc atcagttatg gcattgtcag actgaacgca
    13921 ccggtgactg ccgccttcaa catgaaacag accgtggatg cgttttttga cagcgccagt
    13981 cagaaacaac tgtcagaagc acagtcaaaa gccctttcag cgcgttttaa cacggcactg
    14041 gaagccagcc tgcaggcatg gcagcagaaa caccacgcag tcattctggt gtcgcctgcc
    14101 gtggtacagg gggctccgga tatcacccgg gaaatccagc aggatattgc ccggcgaatg
    14161 agggcggaac catgagatgc cgggggctga ttgccctgct gatatggggg cagagtgtgg
    14221 ccgccgccga tcttggcacc tggggcgatc tgtggccggt aaaggagccg gacatgctga
    14281 cggtgatcat gcagcgcctg acggcactgg agcagtccgg tgagatgggc cggaaaatgg
    14341 atgcctttaa ggagcgggtg atccgcaaca gtctgcggcc ccctgccgtg cccggtatcg
    14401 gacgcacgga gaaatacagc agccggttgt ttgacccgtc cgtcagactg gctgcggata
    14461 tccgggataa cgaagggcgg gtgtttgccc gtcagggtga agtgatgaat ccgctgcagt
    14521 atgtgccttt taaccagacg ctgtatttca tcaatggcga tgatccggcg caggtggcct
    14581 ggatgaaacg ccagacgccg cccacactgg agagcaaaat tatcctcgtg cagggcagta
    14641 tcccggagat gcagaagtct ctggacagcc gtgtttactt tgaccagaac ggtgtgctct
    14701 gccagcgcct cggcattgat caggtcccgg cacgggtgag cgccgttccc ggcgatcgct
    14761 tcctgaaggt ggaatttatt ccggcagagg agggcagaaa atgaagcgaa gactgtggct
    14821 gctgatgtta ttccttctcg ccggtcatgt ccctgcagcg tctgcggatt ctgcctgtga
    14881 ggggcgtttt gtaaacccga tcacggatat ctgctggagc tgtattttcc cgctctcgct
    14941 gggcagtatc aaagtcagtc agggtaaggt ccccgacacg gcgaacccgt cgatgcccat
    15001 tcagatttgt ccggcaccgc cgccgctgtt caggcgtatc gggctggcca ttggttactg
    15061 ggagccgatg gcgctgacgg acgtcacccg atcaccggga tgcatggtta acctgggctt
    15121 cagcctgccg gcttttggta aaacggcaca gggaacggcg aaaaaggatg agaagcaggt
    15181 gaacggggcg ttctatcacg ttcactggta caaatacccg ctgacgtact ggctgaacat
    15241 catcacatcg ctgggctgtc tggaaggtgg tgacctggat atcgcttatc tttctgaaat
    15301 tgaccccacc tggacggaca gcagcctgac caccattctc aatccggaag ctgtcatctt
    15361 tgccaatccg atagcacagg gggcctgtgc agcagatgcc attgccagcg cctttaatat
    15421 gcctctcgat gttctgttct ggtgtgccgg ttcgcaggga agtatgtacc cgttcaatgg
    15481 gtgggtgagt aatgagtcca gtccgttgca gtcctccctg ctggtcagtg aacgcatggc
    15541 gttcaaacta caccgtcagg gcatgattat ggaaaccatc gggaaaaata acgccgtctg
    15601 taatgaatat ccgtccccaa tcctgcccaa agaacgctgg cgttatcaga tggtgaatat
    15661 gtatccggac agcggacagt gccatccgtt cgggcgcagt gtgatgcgct gggagacggg
    15721 aaaaaatccg cccaacacaa agaaaaactt cggctacctg atgtggcgta aacgtaactg
    15781 tgtattcctg taaatcattt taatgaataa gaggaaatga aaatggcaac aatggacagt
    15841 aaatatattc agcacctcag gaaaataagt gctgtactgc tcaggctgat aagcagaaca
    15901 ttaaaattat ttccttttgg tcttgtaatg gtgcccgtta tattatttta ttttgcacct
    15961 gacagttttc tcgctgaaat tattcatgaa tggaagaagg cagatatatc agaacaagtg
    16021 acacttgtca gattatatct gaaggtgaca tttacatgtg cgtttatggc tgtttttctc
    16081 aattatcttt tttctgatga gttcatgcct gtaaatagca gaaagtgaga aatatcatga
    16141 accggaaaat atttgtactt tttgctctga cagcagggat gcatcttgct gttcatgctt
    16201 cagaaaatgt gaacacaact gaaaaccgcc agttcctgaa gcagcaggaa aatttaagca
    16261 gacaactgcg agagaaacct gaccatcagc tgaaagcctg ggcggagaaa caggtgctgg
    16321 aaaaccccct tcagcgttca gataaccatt ttctggatga gctggttcgt aaacagcagg
    16381 cttcgcagga cgggaaaccc cggcagggtg ccctgtattt tgtgtcgttt tccattcccg
    16441 aagaggggct gaaacggatg ctgggcgaaa cccggcacta cggtataccc gccacactgc
    16501 ggggcatggt gaacaatgac ctgaagacca ccgctgaagc cgtgctgtcc ctggtgaaag
    16561 acggcgcgac tgatggtgtt cagatcgacc cgacgctgtt ttcacagtac ggcattcgca
    16621 gtgtaccggc actggtggtg ttctgcagtc agggatacga catcatccgg ggaaacctgc
    16681 gggtcggcca ggcgctggag aaagtggccg ccacgggtga ctgccggcag gtggctcatg
    16741 atttactggc agggaaagga gattccggga aatgaaacgt attttacctc tgatactggc
    16801 tctggttgcc ggcatggcac aggctgacag caacagtgat taccgggccg gttctgattt
    16861 tgcccgtcag attcaggggc agggtacagg cagtattcag ggctttaagc cacaggagag
    16921 tatccccagc tataacgcga atccggacga gacaaaatat tacggtggcg tgactgccgg
    16981 cggggatggc ggcctgaaaa atgacggcac cacggaatgg gcgaccggtg aaaccggaaa
    17041 aaccatcaca gagtccttca tgaacaagcc gaaagacatt ctgtcaccgg atgcaccgtt
    17101 cattcagacc ggcagggatg tggtgaaccg ggctgacagc attgtgggaa ataccggaca
    17161 gcagtgcagt gcgcaggaga taaaccgcag tgaattcaca aactatacct gtgagcggga
    17221 cacgatggtg gaggagtact gcacacggac tgccagtatc accggggact ggaaatacac
    17281 ggatgaatat cgtgaagtga cgataccgca ctcacagttc cggttcagta tgaacgggct
    17341 gaaactggtg ttcagtgtca cggctccggt aaccggtaca gtggaaagtg catcattgtc
    17401 tgtatacgcc gcttttttct tcctgaactc ccgttatacc ttcatgaata caacctttaa
    17461 tgtcggcctg gcaaacgggc agtcggatac ttaccccctg tccggggcga caggtttgca
    17521 ggtgacacag gggcaggttc tgaccggtag tggctgtact gctaacggta attgcctccc
    17581 gcacgggaat ggtgacagga aagtctatga atcgcttgtc agtggtgctt ccacctttac
    17641 gctgaaactc aggatgaagg tccgtgataa agaatgggtt ccccgggtgg agtgggtgga
    17701 gagttgtccg tttaataaag cggatggcgt gctgacaggc actgagtgtt cagagccggg
    17761 tggtacgaaa accggagtga tggaaggtaa accctggaac atcacacagg catgctgggc
    17821 ataccgggat aaatatgtca ctcagtccgc agataacggc acctgtcaga aatatgtcga
    17881 taatccggcc tgtacgctgg cctcacgtca gtgcgcgttt tattccgatg agggaacctg
    17941 tctgcatgag tacgccacgt actcctgcga gtcaaaaaca tccgggaaag tgatggtctg
    18001 tgggggagac gtcttctgtc tcgatggcga atgtgataag gctcagagcg ggcagagtaa
    18061 tgattttgcc gaagccgttt ctcagctggc ggcactggcc gcagcaggta aggatgttgc
    18121 ggcgctgaac ggggtggatg tccgtgcttt caccggtcag gcgaaatttt gtaagaaggc
    18181 tgcagccgga tacagcaact gctgtaagga cagcggctgg ggacaggata tcgggctggc
    18241 caaatgcagc agtgatgaaa aggccctggc taaagcaaaa tcaaacaaac tgaccgtcag
    18301 tgtcggagag ttctgttcga agaaagtgct gggtgtctgt ctgcagaaaa aacgcagcta
    18361 ctgtcagttt gattcgaagc tggcgcagat agtccagcag caggggcgca acgggcagtt
    18421 gcgtatcggg tttggcagtg cgaagcatcc tgactgtcgg gggattacgg ttgatgagct
    18481 gcagaaaatt cagttcgaca gactggattt cactaacttc tacgaagacc tgatgaataa
    18541 ccagaaaatc ccggacagtg gcgttctgac gcagaaagtg aaagagcaga ttgctgacca
    18601 gctgaaacag gcaggacagt aaggtatgga aaataaccgg gggaaatgat gaaagtcata
    18661 ttcacgtcga accgttttat tgattttctt atccggcttt tgattacagc gtttgtcatt
    18721 tccccggtaa ttatctggtc gtgggatacg gtaaaagaaa ccacggcaga cgggatgctt
    18781 gccgctgctt tcgttatcct ttactccggt gtgctcctgt ttattctgta tttttgtttt
    18841 tctgccctga ctgacctgca gaaaaccgat gaaagaaaaa atgatgaaag gaatgaagat
    18901 gaataaagca ttactgccac tgttactctg ctgctttatt tttccggcgt cagggaaaga
    18961 tgcaggctgg cagtggtata acgagaaaat aactccgaag gaaaaagaaa ataaacctgt
    19021 acctgcagcc ccccgtcagg agccggatat tatgcagaaa ctggccgcgc tgcagacggc
    19081 aacaaagcgg gcgctgtacg aagccattct gtatcccggc gtggataatt ttgtgaaata
    19141 tttccggctg cagaattact ggactcagca ggccgggctt ttcaccatga gcgccaaaaa
    19201 agccatgctg gcgcatcccg aactggacta taacctgcag tacagccatt acaacggcac
    19261 ggtccggaac cagctggcgg cagaccaggc gcagcagcga caggccattg cgaaactggc
    19321 tgaacactac ggcatcatgt tcttttaccg ggggcaggac cccatcgacg ggcaactggc
    19381 gcaggtcatt aatggcttca gggataccta tggtctgagt gttattcccg tttccgtgga
    19441 tggcgtgatt aatccgctgt tgccggattc ccggactgac caggggcagg cgcagcgcct
    19501 cggtgtgaaa tatttcccgg ccatgatgct ggttgacccg aaacagggca gtgttcgccc
    19561 gttatcatac ggctttattt cacaggacga cctggcaaaa cagttcctga acgtttctga
    19621 agattttaaa ccgaattttt aaccaaaggt tatatatgca tacaggggat ttgtgttttt
    19681 ttacaggtgc cagcctgttt atcgtggggg ttgttggtta tttctttaca ggaccaaata
    19741 ataaaggagt ttatctgctg agctcgtttg tcttctttgt agtaaatgtc ataagtgtat
    19801 gtatgctgcc agataatttc ttgtttcttt gtggtgcggt tatgaatcag tgtgccgtac
    19861 cggttgtttc aatggcagtc agtaccatac cggttatcac gaagtttgac agtttcagaa
    19921 ggatgtttgt ttgtattatg ttgtcagcca tatggtctgg agtgatgtgg ttttttatta
    19981 tttcattagt gaaatgactg gtgctaattt gtcataaatg ccaaatccaa tagtgaggtt
    20041 gattaccaca gaccagagga taaaccagac tgctatccag tgacttctga ataactcaca
    20101 aaaataaaca tagatgatta gaaatagcca tatcacaaca gcgataatcg ctataaataa
    20161 cagtatcgtc acaaataaat cagcaaaggt tttaatgggg agggagtgca ataatgaata
    20221 caccagtaaa gcaaaagccg cattacctga taatgactct cttatgatgt ttctcactat
    20281 ttccagtttt tctttaaaag ataacttttt catttgtacc tctgattaat atcacacaaa
    20341 cgcgaatatt acagacatga atactctcat gtaaataata accggagttt aacatgataa
    20401 gtaaacgcag attctcttta ccccggcttg atattacggg aatgtgggta ttttccctgg
    20461 gtgtctggtt tcatatcgtc gcccgtcttg tttacagcaa accctggatg gctttttttc
    20521 tggcggagtt gattgcagcc atccttgtgc tgttcggcgc ataccaggtg ctggatgcat
    20581 ggattgcacg tgtcagcctg gaagagcgtg aggcactgga agccaggcaa caggccatga
    20641 tggaagggca gcaggagggc ggacatgtct ctcactaaat tactgccggt cattttgttg
    20701 atgatggcca caggtgtgca ggcctccacc cgggatgaaa ttgaacgact ctggaatccg
    20761 cagggtatgg ccgtgcagcc tgcacaaccg gcagcagaca catcagccag aacagaaaag
    20821 ccggctcccc gctggttccg cctcagcaac ggcaggcagg ttaacctggc cgactggaag
    20881 gtggtgctgt ttatgcaggg gcattgccct tactgtcacc agtttgaccc ggtactgaaa
    20941 cagctggcac agcagtacgg cttttccgtt tttccctaca ccctggatgg tcagggggat
    21001 actgcctttc cggaagcatt accggtgcca ccggacgtga tgcagacctt cttcccgaat
    21061 atcccggtgg ccacaccgac caccttcctg gtcaacgtca acacacttga ggcattaccg
    21121 cttttgcagg gggcaacgga tgccgccagt tttatggcgc ggatggatac cgttctgcag
    21181 atgtacggag aggaaaaagg tacgaaataa gcaggtggtg ttactcattg ccggaatatc
    21241 cggcatcgcg acgggaataa tcgtcagcct gaatatcccc tttatccgtc aggggctttt
    21301 ttatcccgcc agccctgtgg aaattgtcgt ttcgctgtgt cttacctttt ctgtttctgt
    21361 tgtgtttttt gtgggggcaa ttgtgggatg gatttcggtg tctgaaatat attacagccg
    21421 catgaacggt ctgaatgaat catcggaaat atcagaggaa acttataatg aaagaaaaaa
    21481 gaaatgatgc gaaactaaaa aacagaaaaa ctaaacggga ttatgattat gaacgcaggg
    21541 tttctgatat atattttgat ctgttttttg tgttcgttgc tgccggaact tttttatggg
    21601 tgattatgca tagtattttt gatgcctgta ttgattcgtg gaaagctgac ccggaattaa
    21661 ataattttcg ttatatgtgg aacatcctga tgtatgtcat tccgtatacg ttatgggcgt
    21721 ttgcaggtgg atttcttatc gtatacgtca ggaacccttt aaatgaatta ataaacgggg
    21781 ggatcagaat attccggctg aaacgccgta tgcgccggga gaacaaatta agggagggtg
    21841 gcaataatgc ttcgcattaa acctcttctg gttctttgtg ccgctttact gacggtcaca
    21901 cccgcagcgt cagcggatgt gaacagcgac atgaatcagt tctttaataa gctgggattt
    21961 gcttccaata ccacacaacc gggcgtctgg caggggcagg ccgccgggta tgcctatggt
    22021 ggctccctgt atgcccgcac ccaggtcaaa aatatccagc tgatttccat gacgctgccg
    22081 gatatcaatg ccggatgtgg cggcatcgac gcctatctcg gctcgttcag ttttattaat
    22141 ggcgatcagt tgcagcgttt tgttaagcag attatgagca acgctgccgg ttactttttt
    22201 gaccttgccc tgcaggcaac ggtgccggaa atcaaaaccg caaaagactt cctgcagaaa
    22261 atggcaagcg acattaacag tatgaacctc agctcctgtc aggtggcgca ggggattgtc
    22321 ggaggattat ttcctcagac tcaggtgtca cagcagaagg tctgtcagga cattgccggt
    22381 gagagcaata tttttgctga ctgggccgcg tcccgtcagg gctgcacggt cggaggacag
    22441 tcatcgtctg ttcgtgacaa ggccagtgac aaagataagg aacgggtgct gaagaacatc
    22501 aatatcatct gggactcgct gtccaaaaac aggatgcttg atggtaacaa agaactgaaa
    22561 gaatttatta tgacgctcac cggtacgctg atttttgggg aagacagcga gattaccccc
    22621 ctgccggcgc gtaccactga ccgcgatatc ctccgggcga ttatggaggg gggaacggca
    22681 aaagtttatc actgcaacga ttcggacaag tgtctgaagg tggtggcgga tgcaaatgtg
    22741 accatcgccc gggataaagc cctgaaatca cagattacaa aactgctcac cagtattcag
    22801 aataaagcgg tgagtgatac tccactggat gacagggaaa aaggcttcat ttccagcacc
    22861 accgtccccg tctttaaata tcttattgac ccgcagatgc tcggtgtgtc cagcagcgtg
    22921 gtttaccagc tgacggacta tatcggctac gacatcatgc tccagtacat tcaggaactg
    22981 cttcagcagg cccgggccat gattgccacc gggaactatc cacagaccgt gctggacagt
    23041 gtgctggaga atctgaatca ggcacagcaa cagattgcgg ttttccaggg gcaggtaaag
    23101 gtccagcagg atgcattgct ggttgttgac cgtcagatga gctatatgcg ccagcagctg
    23161 tccgcccgca tgctcagccg ttaccagaac aactatcact tcggagggag cacgctgtga
    23221 atgaagtcta tgtgattgcc ggtggtgagt ggttacgcaa taacctgaac gccattgccg
    23281 cctttatggg gacccggacg tgggattcca tcgaaaaaat tgcgctcaca ttgtctgttc
    23341 tcgcggtggc cgtgatgtgg gtacagcggc acaacgtgat ggatttgctg ggctgggtgg
    23401 ccgtgtttgt gcttatcagc ctgctggtta atgtccgtac atcggtgcag attattgata
    23461 acagtgacct ggtccaggtt caccgggtgg ataatgtgcc ggtcggactg gcgatgccac
    23521 tttcactgac gacccgtatc gggcatgcaa tggtggccag ttacgagatg atattcaccc
    23581 agcctgacag cgccacctac agcaagacag gcatgctgtt cggggcgaac ctgattgtga
    23641 aaagcaccga ttttctgtcc cggaatccgg aaatcatcaa tctgtttcag gactacgtcc
    23701 agaactgcgt gctgggggat atttacctga accacaaata cacgctggaa gacctgatgg
    23761 cctccgctga cccctacacg ctgatttttt cccggcccag cccgctacgg ggcgtttatg
    23821 acagcaataa taatttcata acctgtaaag atgcgtcggt cacgctgaaa gacaggctga
    23881 atctcgatac aaagacggga ggcaagacct ggcattatta tgtgcagcag atatttggcg
    23941 gcagaccgga cccggacctg ttattcaggc aactggtgag tgacagttac agttatttct
    24001 acggctccag ccagtctgcc agtcatatta tgcgccagaa cgtcaccatg aatgccctga
    24061 aagagggtat caccagtaat gcagcccgta acggtgatac cgccagcctg gtcagtctgg
    24121 ccacgacttc gtcgatggag aaacagcgtc tggcacatgt ctccattggt catgtgacaa
    24181 tgcgaaacct gccaatggta cagactatcc tgacgggaat tgccattggt attttcccgc
    24241 ttctggtact ggcagctgtc ttcaacaaac tgacactgtc cgtcctgaaa ggctatgtgt
    24301 ttgccctgat gtggctgcag acctggccgt tgttgtatgc cattctgaac agtgccatga
    24361 cattctatgc gaagatgaat ggtgcgccgg tcgtgctgtc tgaactctct cagatacagc
    24421 tgaaatactc tgacctggcc tctactgccg ggtatctttc agccatgatc cccccgttgt
    24481 catggatgat ggtaaaaggc cttggtgcag gtttttccag cgtgtacagc cacttcgcct
    24541 cttcctctat cagtccgacg gccagtgcag caggcagtgt ggttgacggt aattactcct
    24601 acggcaacat gcagacggaa aacgtgaacg gcttcagctg gagcaccaac agcaccacgt
    24661 cgtttggtca gatgatgtac cagaccggca gtggcgcaac tgccacacag acccgtgacg
    24721 gtaatatggt gatggatgca agcggagcga tgtcccggtt accggttggt atcaatgcaa
    24781 cgcgtcagat tgcagcggca caacaggaaa tggcccggga ggcgtcgaac agagcagaaa
    24841 gcgccctgca tgggttcagc agcagtattg ccagtgcctg gaactcgctc agtcagtttg
    24901 gttctaaccg ggggagcagt gattctgtca ccggtggtgc tgacagcacg atgagcgcac
    24961 aggactccat gatggccagc cgtatgcgca gtgcagtgga aagctatgcg aaggcgcata
    25021 atatcagtaa tgagcaggca acaagagaac tggcctcgag aagcacgact gcatcagctg
    25081 gattgtatgg tgatgctcat gccggattag atgcaaagcc cagccttttt ggtgtgcgtg
    25141 ttgatgttgg cgctaaggtt ggtgggcgag ccagtattga cggtagtgat ctggactcac
    25201 atgaagccag tagtggcagc agggcttccc atgatgctcg ccatgatatt gacgctagag
    25261 ccagtaagga ctttaaagag gccagcgatt actttaccag tcgcaaggtc agtgagtccg
    25321 gcagccatac tgacaataat gccgattccc gtgtggacca gctgtctgca gccctgaact
    25381 cagcgaaaca gagttacgac cagtacacga cgaatatgac ccgcagccat gaatatgctg
    25441 aaatggcatc ccgtactgaa agcatgagtg ggcagatgag tgaagatctg tcgcaacagt
    25501 ttgcacagta tgtgatgaaa cacgcaccgc aggatgccga agctattctg acaaacacca
    25561 gttcaccgga gattgcagaa cgccgccggg cgatggcgtg gtcttttgtg caggaacagg
    25621 tgcagcctgg tgttgataat gcctggcgtg aatcccgtgg cgatattggt aaaggaatgg
    25681 agagcgtacc ttcgggtggt ggcagccagg atattattgc tgaccatcag gggcatcagg
    25741 ccattattga tcaaagaacg caggacagta atatccggaa tgatgtaaaa catcaggttg
    25801 ataatatggt cacagaatat aaagggaata taggtgatac tcagaacagt atccgtggcg
    25861 aggaaaatat tgtaagaggg cagtattctg aactacaaaa tcatcataaa acagaagcat
    25921 tatctcagaa taataaatat aatgaagagc gagaaagtca aaagataata cctgagccat
    25981 caggagaggc tgtgaaaaaa atgattgatg ataaaaagga acgactgaag gggccattat
    26041 gatcactcac tatgatataa aaacggagac acagaaacta aaggatgtac tctctgtaga
    26101 gggggttaat atcccgccgt tattacaggt cataaagcct ggtgggtatg tgtttttatg
    26161 ggttttatta tggccgacat ttctccgttt gcaggcagat aaagttgata tccgagatgc
    26221 cggatttgat atatgtttta gtggcgtaat gggttttatc ctttttgttg ccattactaa
    26281 tggaatgatg ttgtatcttg ctatccctaa aaaattcaga gatgagtcga aaattatatc
    26341 ttttatgtat gataagaaca aaacttatat cttgtcattt ttgattgctt tttcaatggt
    26401 ttcatttgcc catacactct tatatgagtt tttattaatt gctttgttta taatcttttt
    26461 ctttatttat gctatagaca taaatcgtta taatctctca gctatagtct cagtaatagg
    26521 actgttcaaa aaagagtctg ttagttaaaa ggatattctg gatgaaaaca aaaaaaatga
    26581 tggtggttgc actggtcagt tccactctgg ccctttcagg gtgtggtgcg atgagcacag
    26641 caatcaagaa gcgtaacctt gaggtgaaga ctcagatgag tgagaccatc tggcttgaac
    26701 ccgccagcga acgcacggta tttctgcaga tcaaaaacac gtctgataaa gacatgagtg
    26761 ggctgcaggg caaaattgct gatgctgtga aagcaaaagg atatcaggtg gtgacttctc
    26821 cggataaagc ctactactgg attcaggcga atgtgctgaa ggccgataag atggatctgc
    26881 gggagtctca gggatggctg aaccgtggtt atgaaggcgc agcagttggt gcagcgttag
    26941 gtgccggtat caccggatat aattccagtt ctgccggtgc cacactcggt gtgggccttg
    27001 ctgccggtct ggtgggtatg gctgcagatg cgatggtgga agatgtgaac tataccatga
    27061 tcacggatgt gcagattgca gagcgtacta aggcaacggt gacaacggat aatgttgccg
    27121 ccctgcgtca gggcacatca ggtgcgaaaa ttcagaccag tactgaaacg ggtaaccagc
    27181 ataaatacca gacccgtgtg gtctcaaatg cgaacaaggt taacctgaaa tttgaagagg
    27241 cgaagcctgt tctcgaagac cagctggcca aatcaatcgc aaatattctc tgaacctcgt
    27301 caggaggctg atctgaccgg cctccttgct gacgcctcgc gttgctcgtt gtccaacccc
    27361 gggatgtaac aaaataaatt ttgtcacctc ccggcgtttc cgaaataaat atgaaaacac
    27421 tgagcggttg tgcaaattct ttgtgcgacc gtgaagtgtt gccggcgaag ccgggatctt
    27481 ccggagaaag tcattatgac atcagacaat atcgctgaat atgttggtga gaataatcaa
    27541 tttactgaga agcctgtaac tgaatacccc ccggtgatac aggataatac agtgaccact
    27601 aaagactatc tgtacagcaa taatattgat actgaccttt tagtggttat cggagggctt
    27661 attgttatat tgtttctcat gatgcttatc atgtacttta ttaattctct caggcataag
    27721 gaattaactg aaatactgac aaaaatatct tttcctgaaa aatccgggag caatttgctt
    27781 aaaccggagg cggtcagctg taatttgttg tccctgtgtg tgctggcacc ggtattctgt
    27841 acacccagaa agctggctga agtttccagg gagatcatcg tggcgagtgc gcaggaaatt
    27901 gcatggacgc cggggagcag tcgtattgat ggccaccagg cggagaatat cacctgggcg
    27961 atttcatcgc agtctcctgc cgataagtat gatctcatca ataaaacgtc ctgtgtttct
    28021 cctgacagtg gccgttttta tgaaccggac acagttctgg atcaccgtga acgggtacgc
    28081 ctttttattc agatccagtt tccgcatggg agtgactggt ctgaagtatt gcgcctgact
    28141 gacattgtta cagagaaggt ctcttccggc aggactgcag gcgggcatca ccaccggcat
    28201 acagggtaca cctggtctgt ttctgtgaat taaccccaca tcatcctttt ctgccctgtt
    28261 ccagattttc ccggagtttc atcatgagtt ttaacgcaaa ggatatgacc cagggcggtc
    28321 agattgcgtc catgcgtatc cgcatgttca gccagatcgc caatatcatg ctttactgcc
    28381 tgtttatttt tttctggatc ctcattggtc tggtcttatg ggtaaaaata agctggcaga
    28441 cgtttatcaa cggctgtatt tactggtggt gtacgtcact ggaaggcatg cgggatttaa
    28501 taaaatccca gccggtctat gagatccagt attacggcaa aaccttccgg atgaacgctg
    28561 cccaggtact gcatgataaa tatatgatct ggtgcggaga gcagctatgg tctgcattcg
    28621 ttctggcgtc tgtcgtggcc ctggttattt gcctgatcac cttctttgtt gtctcctgga
    28681 ttctggggca tcaggggaaa cagcagagtg aaaatgaggt gaccggcggg cgtcagctga
    28741 cggataaccc gaaagaagtg gcccggatgc tgaaaaaaga cggcaaggac tccgatatcc
    28801 ggattggcga cctgccgatt atccgggatt ccgaaatcca gaacttctgt ctgcacggca
    28861 cggtcggggc cggtaagtcg gaggttatcc gtcgtctggc caactacgcc cgtcagcgcg
    28921 gtgatatggt ggtgatttat gaccgttcag gggaatttgt taaaagttac tatgatccct
    28981 ccatcgataa aatcctgaat ccgctggatg cccgctgtgc cgcatgggat ttgtggaagg
    29041 agtgcctgac acagccggat tttgataata ccgcgaacac gctgatccct atgggtacaa
    29101 aagaggaccc gttctggcag ggttcaggac gtaccatttt tgcggaagcg gcgtacctga
    29161 tgcgtaatga ccccaaccgc agctacagca aactggtgga cacactgctt tccatcaaaa
    29221 ttgaaaaact gcgtaccttc ctgcgtaatt caccggcagc caacctggtg gaagagaaaa
    29281 ttgagaaaac ggcgatttcc atccgtgctg tgctgaccaa ctacgtgaaa gccatccgtt
    29341 acctgcaggg gattgagcat aacggtgagt cttttaccat ccgtgactgg atgcgtggtg
    29401 tccgggaaga tcagaaaaac ggctggctgt ttatttcgtc gaacgccgac acccatgcct
    29461 ccctgaagcc ggtgatctcc atgtggctgt ccattgccat tcgtggtctg ctggcaatgg
    29521 gagaaaaccg taaccgtcgt gtgtggtttt tctgtgacga gttacccacg ttacacaaac
    29581 tgccggacct ggtggagatc ctgccggaag cccgtaagtt cggtggctgt tatgtgtttg
    29641 gtatccagtc ctatgcccag ctggaagata tctacggtga gaaagcggcg gccacgctgt
    29701 ttgacgtcat gaacacccgt gcctttttcc gttctcccag ccataagatt gcagagttcg
    29761 ctgccggtga aattggtgag aaagagcacc tgaaagccag cgagcagtat tcctacggtg
    29821 ctgacccggt acgtgacggg gtatcgaccg gtaaggatat ggagcgtcag acgctggtca
    29881 gttattccga cattcagtct ctgccggatc tgacctgcta tgtcactctg cccggaccgt
    29941 atccggcagt caaactctct ctgaaatatc aggcacgacc gaaggtcgct ccggagttta
    30001 ttccgcgtga catcaacccg gaaatggaga accgtctgag tgccgtactt gctgcaaggg
    30061 aagcagaagg tcgccagatg gccagcctct tcgaaccgga ggttgcctcc ggagaaggcg
    30121 tgactcaggc tgaacagcca caacagccac aacagccaca acagccagtg tcttctgtca
    30181 tcaatgataa gaagtcagat gcaggtgtga gtgttccggc aggggggatc gagcaggagc
    30241 tgaaaatgaa accggaagaa gagatggaac agcaactgcc acccgggatc agtgaatccg
    30301 gtgaagtggt ggatatggcc gcttatgagg catggcaaca ggaaaatcat ccggacatcc
    30361 agcagcagat gcagcgtcgt gaagaggtga acattaatgt gcaccgggag cgtggggagg
    30421 atgttgagcc gggagatgat ttctgatgat gagtatcgcg caggtcagat cggccggaag
    30481 tgccggtaac tattataccg acaaggataa ttactatgta ctgggcagca tgggagaacg
    30541 ctgggccggc aggggggctg aacagctggg actgcagggc agtgtcgata aggatgtttt
    30601 tacccgtctt ctggagggaa ggctgccgga cggagcggat ctaagccgca tgcaggatgg
    30661 cagtaataag catcgtcccg gctacgacct gaccttctcc gcccccaaaa gtgtctccat
    30721 gatggccatg ttaggtggcg ataagcgcct gattgatgca cataaccagg ccgtggactt
    30781 tgctgttcgt caggtggagg cgctggcctc cacacgggtg atgacggacg gacagtcaga
    30841 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
    30901 ggaaccacag ttacacacgc atgcggtggt ggctaatgtc acgcagcata acggtgagtg
    30961 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
    31021 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
    31081 ctatgaaacc gaagtggtgg gtaagcacgg tatgtgggag atgccgggcg taccggtgga
    31141 ggccttttcc ggacgctcac aggctatccg ggaggccgtc ggggaagacg cctcgctgaa
    31201 atcccgggat gtggcggcac tggatacgcg taaatccaaa cagcacgtcg atccggaaat
    31261 cagaatggct gaatggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
    31321 tgacgcggcg gatcagcgtg cggagacccg cacgcaggcg cccggggctg tttcacagga
    31381 ggggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgaaaagt
    31441 gcagtttacg tacacggacg tgctggccag aacggtcggt atactgccgc cggaaaatgg
    31501 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agtcgtgagc agcttatccc
    31561 cctcgaccgt gagaaggggc tgttcacatc aggaattcat gtgctcgatg agctgtccgt
    31621 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
    31681 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctgg
//
LOCUS       DADLCQ010000040        31572 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000040 DADLCQ010000000
VERSION     DADLCQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.040"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            698..1126
                     /gene="uspC"
                     /locus_tag="JQG95_001663"
     CDS             698..1126
                     /gene="uspC"
                     /locus_tag="JQG95_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY5888254.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASNPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="JQG95_001664"
                     /pseudo
     CDS             complement(1133..2547)
                     /gene="otsA"
                     /locus_tag="JQG95_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
     gene            complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="JQG95_001665"
     CDS             complement(2522..3322)
                     /gene="otsB"
                     /locus_tag="JQG95_001665"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY5888255.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(3489..4478)
                     /locus_tag="JQG95_001666"
     CDS             complement(3489..4478)
                     /locus_tag="JQG95_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY5888256.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGIPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4490..6004)
                     /locus_tag="JQG95_001667"
     CDS             complement(4490..6004)
                     /locus_tag="JQG95_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY5888257.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6074..7063)
                     /locus_tag="JQG95_001668"
                     /pseudo
     CDS             complement(6074..7063)
                     /locus_tag="JQG95_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
     gene            7860..8363
                     /locus_tag="JQG95_001669"
     CDS             7860..8363
                     /locus_tag="JQG95_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY5888258.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8443..8694)
                     /locus_tag="JQG95_001670"
     CDS             complement(8443..8694)
                     /locus_tag="JQG95_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY5888259.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="JQG95_001671"
     CDS             complement(8809..8895)
                     /gene="azuC"
                     /locus_tag="JQG95_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY5888260.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9158..9481
                     /locus_tag="JQG95_001672"
     CDS             9158..9481
                     /locus_tag="JQG95_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888261.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9652..10149
                     /gene="ftnA"
                     /locus_tag="JQG95_001673"
     CDS             9652..10149
                     /gene="ftnA"
                     /locus_tag="JQG95_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY5888262.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10186..10425)
                     /locus_tag="JQG95_001674"
     CDS             complement(10186..10425)
                     /locus_tag="JQG95_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY5888263.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10615..11826
                     /gene="tyrP"
                     /locus_tag="JQG95_001675"
     CDS             10615..11826
                     /gene="tyrP"
                     /locus_tag="JQG95_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY5888264.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="JQG95_001676"
     CDS             complement(11888..12553)
                     /gene="yecA"
                     /locus_tag="JQG95_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY5888265.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12749..12835)
                     /locus_tag="JQG95_001677"
     tRNA            complement(12749..12835)
                     /locus_tag="JQG95_001677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Leu,seq:taa)
     gene            complement(12848..12921)
                     /locus_tag="JQG95_001678"
     tRNA            complement(12848..12921)
                     /locus_tag="JQG95_001678"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12887..12889),aa:Cys,seq:gca)
     gene            complement(12976..13051)
                     /locus_tag="JQG95_001679"
     tRNA            complement(12976..13051)
                     /locus_tag="JQG95_001679"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13016..13018),aa:Gly,seq:gcc)
     gene            complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="JQG95_001680"
     CDS             complement(13203..13751)
                     /gene="pgsA"
                     /locus_tag="JQG95_001680"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY5888266.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="JQG95_001681"
     CDS             complement(13808..15640)
                     /gene="uvrC"
                     /locus_tag="JQG95_001681"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY5888267.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="JQG95_001682"
     CDS             complement(15637..16293)
                     /gene="uvrY"
                     /locus_tag="JQG95_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY5888268.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVAGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16589..16765
                     /locus_tag="JQG95_001683"
     CDS             16589..16765
                     /locus_tag="JQG95_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888269.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            16752..16976
                     /locus_tag="JQG95_001684"
     CDS             16752..16976
                     /locus_tag="JQG95_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY5888270.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="JQG95_001685"
                     /pseudo
     CDS             complement(17009..17629)
                     /gene="sdiA"
                     /locus_tag="JQG95_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="JQG95_001686"
     CDS             complement(17859..18611)
                     /gene="yecC"
                     /locus_tag="JQG95_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY5888271.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="JQG95_001687"
     CDS             complement(18608..19276)
                     /gene="tcyL"
                     /locus_tag="JQG95_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY5888272.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="JQG95_001688"
     CDS             complement(19291..20277)
                     /gene="dcyD"
                     /locus_tag="JQG95_001688"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY5888273.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="JQG95_001689"
     CDS             complement(20382..21182)
                     /gene="tcyJ"
                     /locus_tag="JQG95_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5888274.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="JQG95_001690"
     CDS             complement(21270..21821)
                     /gene="fliZ"
                     /locus_tag="JQG95_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY5888275.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(21867..22586)
                     /locus_tag="JQG95_001691"
     CDS             complement(21867..22586)
                     /locus_tag="JQG95_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY5888276.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22751..24403)
                     /locus_tag="JQG95_001692"
     CDS             complement(22751..24403)
                     /locus_tag="JQG95_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HAY5888277.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24669..26075
                     /gene="fliD"
                     /locus_tag="JQG95_001693"
     CDS             24669..26075
                     /gene="fliD"
                     /locus_tag="JQG95_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY5888278.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            26100..26510
                     /gene="fliS"
                     /locus_tag="JQG95_001694"
     CDS             26100..26510
                     /gene="fliS"
                     /locus_tag="JQG95_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY5888279.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADVWKESLLSPSSIQDPV"
     gene            26510..26875
                     /gene="fliT"
                     /locus_tag="JQG95_001695"
     CDS             26510..26875
                     /gene="fliT"
                     /locus_tag="JQG95_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY5888280.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26953..28440
                     /gene="amyA"
                     /locus_tag="JQG95_001696"
     CDS             26953..28440
                     /gene="amyA"
                     /locus_tag="JQG95_001696"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY5888281.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28474..28887)
                     /locus_tag="JQG95_001697"
     CDS             complement(28474..28887)
                     /locus_tag="JQG95_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5888282.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            29074..30279
                     /gene="yedE"
                     /locus_tag="JQG95_001698"
     CDS             29074..30279
                     /gene="yedE"
                     /locus_tag="JQG95_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY5888283.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFPRE
                     APQTAKEIA"
     gene            30276..30509
                     /gene="yedF"
                     /locus_tag="JQG95_001699"
     CDS             30276..30509
                     /gene="yedF"
                     /locus_tag="JQG95_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY5888284.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30616..31286
                     /locus_tag="JQG95_001700"
                     /pseudo
     CDS             30616..31286
                     /locus_tag="JQG95_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(31326..31571)
                     /locus_tag="JQG95_001701"
     CDS             complement(31326..31571)
                     /locus_tag="JQG95_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5888285.1"
                     /translation="MLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVN
                     GARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7942 a         8063 c         7461 g         8106 t
ORIGIN      
        1 ttagcattag aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa
       61 gtccttacaa atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
      121 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
      181 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
      241 tgggtgtatt tcaatctgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
      301 gaataatcga aatgggatgt tgcgcacggt taaaataact caccgtaaat aatcatctgc
      361 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
      421 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat aaaatgtttt
      481 tcatatatta accacttgtt tgatgcgggg aatcagtaca ttattttctt ataattacag
      541 ttgaaatcat atgatcaccg agagtgattg attaattgtc tttactaata atcagactaa
      601 tatttacctg tttgactgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
      661 tgtcacctga agttcacagc agaataagga acaggttatg agctatagca atattcttgt
      721 cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag
      781 gccagtaaag ggacacatca gtttaattac tctcgcttcc aacccggaaa tgtacaatca
      841 attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt
      901 tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg
      961 tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcaatt tggtgatttg
     1021 cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc
     1081 ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa
     1141 gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct
     1201 gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt
     1261 ccagcatttc tgcatgacgg gaaatacgtt ccaccagcga catagtcaat gcacgatcca
     1321 gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt
     1381 ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag
     1441 caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt
     1501 cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa
     1561 gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct
     1621 gatgacgaat atcctggtag gcttgcacat caccacgcga cgttggtgca atctgggtat
     1681 aacgaatttt accatgatgc tgcggatatt tttccagcaa tgcctcatag gcgagaaaac
     1741 gctccggcaa acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt
     1801 tcaattccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta
     1861 tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttt ccccaggctg
     1921 tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaaggcaa tccaggaacg
     1981 ccagacgatc gttttctgtc tgtaaaccca gcaaatcata atcacaaagc tgttcaagca
     2041 aggtgtcata tgtcggcagc gcgttgaaaa tttccggtgt cgggaaagga atatgcaaga
     2101 aaaaaccaat gcgattattc accccacgtt tgcgtaattc atgcgcaaag ggcaacaggt
     2161 gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca
     2221 gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga
     2281 gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa
     2341 ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt acctttttta gcggctgatc
     2401 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
     2461 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
     2521 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
     2581 gtaatgcggc ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
     2641 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
     2701 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
     2761 aaggagcttc ctgcataaag gctgcaatcg cttcaccttt actggtacct ctcggtttga
     2821 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
     2881 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
     2941 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgaccgta tgcagttgca
     3001 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
     3061 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
     3121 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
     3181 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
     3241 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
     3301 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gtgttgcgca
     3361 gaccgcgctt tacagacatg taagtataga cagtgtgacg gaggtcgcca tttgcaacga
     3421 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
     3481 aaaaagcatc agacagtgcg cttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
     3541 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
     3601 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
     3661 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
     3721 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
     3781 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tatacccgcc
     3841 agacgcgcag cctcttcgtt cccgccaatc gccagggtat tacgaccaaa ggtggtttta
     3901 ttcagcagca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
     3961 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
     4021 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgtcgtgatc
     4081 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaacgccc
     4141 agcaacaaac ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
     4201 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
     4261 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
     4321 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
     4381 gcaaacacca ccagcatgcc gtactgatcc cagatacgcc cgaagctgaa tgacgactta
     4441 ggtgcgccag accccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
     4501 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
     4561 acaattcacc ggcgatttcg ccttcccgca tcaccacaat ccggtcggca acgccgagga
     4621 cttcaggtaa gtcgctggag gcaaacagca ccgccacgcc ctgcgccgcc agcgcataaa
     4681 ttacgttata tatttcgtgc ttagcgccaa catcaatgcc gcgcgtaggt tcatccagca
     4741 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
     4801 ctgagagatt catgatcagt tgctccgcgc ccggcgtttt gatgttgagc gaacgaatgt
     4861 gctgatcggc attgttttct tcccaactgt tgttgattac acaaccgccg agcacatgtt
     4921 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
     4981 ctttgcgagc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
     5041 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
     5101 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
     5161 cactgcgaac cgccagactt attggcgtac gcacgcctag tgctttcaca gcatcaagac
     5221 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
     5281 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
     5341 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
     5401 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
     5461 gattgccgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
     5521 cgttacgcgc cagcgctttg gcgatttcaa ccatctgcca ctgaccaatg gcgagatatt
     5581 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
     5641 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
     5701 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
     5761 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
     5821 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
     5881 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
     5941 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
     6001 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
     6061 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
     6121 cacgcgtgat cagtaccacg tctgtaactt cggtaaattt tggcgtttca acgtcttttg
     6181 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
     6241 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gcttactgca tccacaccgt
     6301 taataccaat accgatgata tcggctgctt taaagccctg accttcggtt gcacgtacgc
     6361 cgccaagtac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
     6421 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
     6481 cctgataaat ttgtttttcc gggaatcctg cagctttcag tgcgtccata gaacccgttg
     6541 tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc catcactgcg ctttctttga
     6601 catcccagcc acgtttctgc atctctttat acagttccta gccctgacgt tcgccaattt
     6661 tagtcgccgc catcatcacc agaggaacgg tatccattgg cttacctttg gcgttaacaa
     6721 actggtcatc cactgcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
     6781 agccgagttt cgggtccgga gtacaaataa cgaaaccttt tgcgccactg gctgccaggc
     6841 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
     6901 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
     6961 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
     7021 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
     7081 gtgtcgtttt gtgtagtgca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc
     7141 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
     7201 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
     7261 ggaaaagaca taaggaaaag ccaatttgtc agacaaattg ttgaatgcac agcagattaa
     7321 tccataagat tagcctggaa atccttgttg tcttcggtac ccattcggga tgtcttcttt
     7381 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccac
     7441 aactcaaaga tgtggaaaat gcgcgtcatt catttcgcca ttaattatca ctgtgctcat
     7501 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
     7561 atcatcactt catcatgaat tcaaattcat cgattaatat caacaagata caaaaagcac
     7621 tatcattaaa attcattgca tttacattga tttcaccaat gaaatgtaaa aatatataaa
     7681 cttgatgatt taagcatttt cttataaccg ttcagacgtt attcttattt cagatcatcg
     7741 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
     7801 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
     7861 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
     7921 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
     7981 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
     8041 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttccaggt gaaaaactga
     8101 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcactc
     8161 tggcgcagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
     8221 gcgatctgga aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag
     8281 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
     8341 tcgtgtcaca ccagctgcat tgatcataca tcggcgctaa tacattgcgc cgatgaagtt
     8401 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
     8461 tggggtaaac ttgagttcaa tcagcgcgat ggctttttgg attgcccgca tggtgaccgg
     8521 gtctgcggca gcgggatgat gagtaaagtc gatattcttc agctgactgg acattttttc
     8581 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
     8641 gcaagctacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     8701 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     8761 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     8821 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     8881 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     8941 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     9001 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     9061 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc
     9121 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     9181 ttcattaata acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga
     9241 cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac
     9301 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     9361 cgcgatcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     9421 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     9481 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     9541 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     9601 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     9661 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     9721 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     9781 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     9841 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     9901 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     9961 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcagtggtat
    10021 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
    10081 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
    10141 caaaactaat gctctccggc ggcaggtttc tgccgccgtt tcaaatcagt gacggcaaat
    10201 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    10261 aaacgctacc agctctcccg ctgtcacccc ttctgccgag cacgtgtgaa aacgtgcctg
    10321 ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca tgcgtatatt gctcgcctga
    10381 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
    10441 aaagcctagc gtagcgatta ccgcttatga agactttgcg ccagcgcagg actgaatgct
    10501 tttattgtac atttatattt acacaatatg taacgtcggt ttgacgaagc agccgttatg
    10561 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
    10621 aacagaaccc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
    10681 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
    10741 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
    10801 gcagataccg gtctgggcac gctggcaaaa cgctatctgg gacgctacgg tcaatggctg
    10861 acgggcttca gtatgatgtt cttaatgtat gctctgactg cagcatacat cagcggtgcc
    10921 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
    10981 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgttggaac atcgctggtc
    11041 gatttattta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
    11101 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
    11161 gcactgtctg caatcccggt gatttttacg tcgtttggtt ttcacggtag cgtgccgagt
    11221 attgtcagct atatggatgg caacattcgt aagctacgct gggtgtttat aatcggtagt
    11281 gccatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
    11341 acaaccttta tgggattgct ggctaatcat gctggattaa acgggctgtt acaggcgtta
    11401 cgcgaaatgg tggcctctcc gcatgttgag ctggcggtgc atttatttgc tgatttagcc
    11461 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
    11521 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
    11581 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
    11641 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
    11701 aatcctcagg cgggttaccg ggtcaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
    11761 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg caggcttgtt accagaagtg
    11821 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatg
    11881 tgacctctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
    11941 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
    12001 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
    12061 caaacgcttc tggcgacatt ttctctaccc gctcgaagtt ttcctcagta ccgtgcagcg
    12121 caatcgcctc cagcgctggt tttaaagaat cgggcaacgt tgaccagtca gaaagtgcca
    12181 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
    12241 ctcgcaagcc gaataacggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
    12301 cggccatatg ttgaaaagcc aggttcataa agcgcgtcat ctctttctct gacgcccaac
    12361 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgttctggt tcaatctctt
    12421 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
    12481 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
    12541 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
    12601 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
    12661 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
    12721 tactttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
    12781 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
    12841 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
    12901 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgacgc
    12961 cgatgtcttt taaactggag cgggaaacga gactcgaact cgagaccccg accttggcaa
    13021 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
    13081 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
    13141 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt acgccgaaac
    13201 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
    13261 gtcagtaccg cagccacaaa gaaaagtgca ataccgacgt actcaaccca aatgttcgga
    13321 cgccacagca gccaggccaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag
    13381 gagacggcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
    13441 ataatttcac gggcgatcat cgttgccgcc ggtaatgtca cccaccagct gtgataatgc
    13501 tcggttacca gcaccatggc gatagccacg agaactttat ctgccacagg gtcaaggaaa
    13561 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
    13621 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
    13681 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
    13741 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
    13801 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
    13861 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
    13921 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
    13981 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcatgt gattcatcgc
    14041 gaatatgctg gataacatgc agcgcgggtg aatctggcgg caaactaaat ccctcacctt
    14101 ccggctcaaa gaacagcgtt tccagtccag ccttacgatc tgctcctttg gcaacgccaa
    14161 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
    14221 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
    14281 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
    14341 tgatgcctgt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
    14401 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
    14461 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
    14521 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
    14581 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
    14641 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
    14701 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tctaccacct
    14761 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
    14821 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
    14881 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
    14941 gaatttggtc gcgaatacat gccgcttctt caaactccag attctggctg gcagtttcca
    15001 tacggctaat gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
    15061 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggtcccagac
    15121 agcgccctat ctggtattgc agacacggac gcgagcgatt gcgataaaca ctattttcgc
    15181 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
    15241 tcgggaacgg gccgaaatat tcacctttgg catgctttgc accacgatgc atcgccagac
    15301 gcgggtgggt atcgccactc aggaagataa aaggataaga tttatcatcg cgtagcaaaa
    15361 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
    15421 ctgtatgcgt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
    15481 gcgaagcgag gttgctgcgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
    15541 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
    15601 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
    15661 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
    15721 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
    15781 tgagatctca ttgaccttct ggcccttagt gatcatcagc ataatctgca attcacgttc
    15841 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
    15901 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
    15961 gacttcctgc ggagccgcgc ctttgctgag gtagccagca gcacctgcct gcatgacttt
    16021 cgctggtaaa gggttttctg tatggaccgt aagcatgatg attttaacat cagctgtgga
    16081 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
    16141 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
    16201 tccggcgact tttataccct taatatcttc cagaatgcgt cgtatccctg cgcgcaccag
    16261 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
    16321 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
    16381 aatggtgggt attcgatttt tctctcgttt ttgttaattc gattgtccac agttatggtc
    16441 acgcccgtca ccaacggctt accggatcgt ttcacagtaa gccctacgat gaaatgtgac
    16501 aaaaattgac tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
    16561 acccgaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
    16621 cgtattttat agcactgccg atatcacgct caaaaaacaa ccactgctat ttttagcaaa
    16681 gcttatggta tactccgccg ccttaacatt tttcaccaca aattttcatt gcaacatgac
    16741 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
    16801 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
    16861 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
    16921 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
    16981 aaaccggctg agagcgtgcc tttcagccgg ttattgacaa aatcatcgct atctctgctg
    17041 atgtcttccc ttccgccgtc catttcagaa tttctttttc gcgcttgctg aaattcatct
    17101 ctggcgtcat cactatttca tcatttaaac gcatcagagc catcagactt tcgcgcacca
    17161 gtaactgcat ttttaattgc agttcatcac taagaatggg tatttcgcgc gtgctgcaac
    17221 gggaaaagga caaaaagccc agcgcccgat ttggcagcat taaatactga gtgacaccgc
    17281 ggcgtaaacc atgtgcgtgc gcggcttccc ataatggctg tgcttcgctg aataagtcat
    17341 cattccacat taaatggccc tgactaaagt tttcagggtt cagcacagga tcaattgcga
    17401 gaaaattttt tgcctgataa taactaaccc acgactcagg gtaattggtg tagaaagcca
    17461 ctttaggtcg cgtgaatggt accgggtggc gaacacataa cgaatagtaa tcgtactcca
    17521 gctgctgagc ctgaagctct atttcatggt aggcctcttc tgcggcctcc atcctctgaa
    17581 aacgcaacag catcgtgcga cgccagctga aaaaatccgt atcctgcata gtaaaccgca
    17641 acgcccctga gagtgagtat catttataat gataattcaa actatcacat aaataagatt
    17701 tatatataat ttatattatt caggcaatga attacttttg caagccatcg cattctctta
    17761 tgttattaat gagttatgct gatttgttaa gcagttttat cacgcttgaa atggcgtccc
    17821 gccccgacag gtgaatcgtc ggggctgatt ttttcttatt attgcagcag aagcttctcg
    17881 aggaactggc gggtgcgagg ctgctgggga tcggcaaata acgcttttgc tgccccctgc
    17941 tcgactatcc gcccctggtc cataaagatc gcccggtccg caacatcccg ggcaaagctc
    18001 atttcgtgtg tcacaatcac catcgtgcgc ttttcctgcg ccagctggcg gatggtgttc
    18061 aggacttcgc ccaccagctc tggatccagc gccgacgttg gctcgtcaaa caaaatcacc
    18121 tcaggacgca ttgccagtgc acgcgcaatc gcaacacgct gctgttgacc gccagacaaa
    18181 cgacgtggat agctggtttc tttaccggcc agcccaactt tcgccagcag ctcgcgagcg
    18241 cgcgccgtgg cctcttcttt cggttcgcct ttgacgatca ccggcccttc aataatgttt
    18301 tccagcaccg tacgatgcgg aaacaaatta aagttctgga agacaaaccc gacgtgctga
    18361 cgcaactggc gaatcagcga tttttgctga cttaatgaac gtgcagtatc aatagtgata
    18421 tcgccaacgg tgatcgttcc cgcttccggt tgttccagca gatttatgct acgtagcaat
    18481 gtggttttgc cggaaccact cggaccgata attgccacca cttcgccagg ctttacctcg
    18541 aggtcgatac cgtgcagcac cgtctgaccg tggaattttt tcaccaggtt cttaacttca
    18601 atggcactca ttttggttct ctctcctggc gattaagttg attctcaaaa tggttctgca
    18661 acgtcgataa cacagtcgcc atgatccagt agatgagcga agccgccagg tacatggtga
    18721 acacctccag cgtgcgcgag gtaatcaact gcgcctgacg gaacagctcc ggcacctgga
    18781 ttgtcgcggc cagcgacgta tcttttacca gactgataaa gctgttcgac agcggcggca
    18841 acgccacccg cgccgcctgt ggcaaaatcg cgcgacgcat ggtctgccac ggcgtcatac
    18901 caatactggc agcggcttcc cactgacctt tatcaataga agaaatggcg gcgcgcagcg
    18961 tttcggcggc ataggcggca gtgttcagcg acagaccaat cattgctgac ggaatgggat
    19021 ctaattcgat accaaactgc ggcaggccgt aatagatcat aaacagttgt gcgatgagcg
    19081 gcgtaccacg aaaaatagag atataaaaac gcgccagcca gcgcacgggc cagattggcg
    19141 acaggcgcat cagcgcgaga ataaacccga gtagtaagcc aaaaaacatg ccgccgatac
    19201 tgagttgcag ggtataccca gcccctttca acaggaacgg caaggaatca ataaccagtt
    19261 gtatactttc ttgcatgagc gttcccggag ctaaacgtgg ggatgatagg cgaacagcgc
    19321 aggcgcgccg ccggtatgaa taaacagaat cggcccttca tctttgaagc gtttctgact
    19381 gataccgtca atcagccccg ccatcgcttt tccggtatac acaggatcaa gcagaatgcc
    19441 ttccagacgc gccagcaatt tcactgcttc catgccttcg tcgttcggca cgccgtagcc
    19501 aggtgcaaaa tagtcatccc agagtaaaat ttccgctgat gcggtcagct ccagttcttt
    19561 cgcaatcgcc tgttgtaggt taaccacttt cggcaattga tcggcaacgg aacgcgacac
    19621 ggtcacgcca atcagttcgc tttcaggcat caggtgttcc agcccaacag ccagtccggc
    19681 gtgagttccg gcactgcccg atgcgactac caccgacgaa atattaaccg ccccttcaca
    19741 ctgttgcgcg atttccagcg cactctccac ataacctagc gcgccaagag cattagaacc
    19801 gccaaccgga atgacatacg ggcgaaagcc ttgtgcttcg actcgcgtcg ccagttcttc
    19861 cagttgggca ttgggatcgg tcagtgcgtc gcacatttca atctgggtat tgaacagatc
    19921 caacaacaaa cgattgccgt tggttaaata gttttctgcg gttgtgccaa taggattttc
    19981 cagcagcgcc acgcagtgca gaccgagttt cgccgcgact gcggcagtct ggcgcacatg
    20041 gttagactgg atcgcgccgg cagtaatcag cgtatcggca ccttcgcgca gggcatctgc
    20101 cgcgagaaat tccagcttac gtaatttatt gccgcccatt gccatggggg tgacctcatc
    20161 ccgtttgatg aaaatttccc gtccaagata atcagaaaag cgcggcagat attcgagcgg
    20221 cgttggcgcg ccgataaact ccaaccgtgg aaaacgggtt aaattatgca gtggcataac
    20281 agcctccgat gtgtgttgtt gtgattttct tattatgcac gctgaaaacg cgtaaataaa
    20341 aaaggcgcta gtgaaagcgc ccttttttgt cattatgctg attatttggt cacatcagca
    20401 ccaaaccatt tttcggaaag agcttgcaga gtgccgtctt tttgcatttc cgcaattgca
    20461 tcattcaccg ctttcagcag gtcttcattt cctttacgca gcgccacgcc agactcctga
    20521 cgggagaatg cttcaccggt tactgccagc gtatcgttgg ttttcttcac cagatccagc
    20581 gccgccagac gatcaacgag gatcgcatcg atacgcccta cgcgcagatc ctgatatttg
    20641 gtcgggtcat catcataggt acgcacatcg acaccctgaa cattctgccg cagccactct
    20701 tcatagttgg tgcccagacc gacacccact tttttgcctt tcagatcagc agccgtttta
    20761 atggtgcctt cgttaccttt tttcaccagc gcctgaatac cggaaatggt gtaaggggta
    20821 gagaaatcgt attttttctt gcgctcgtca gaaatggtga cctgattaat caccacatca
    20881 atgcgtttag agtccagcga cgccagcata ccgtcccatt tggtcggttt tagtgacgcc
    20941 tcaacgccaa gatgttttgc cagctgttgg gcaaattcca cttcaaaacc ggttaatttg
    21001 ccgtcatccc cctgaaaact gaacggcgga taagtgcctt ccagccctac cagcagcgtg
    21061 ccgctctctt tgactttatt aagcagacct tcatctgcaa aacttttaac gctcatgccc
    21121 gcaaccagcg ccacggccat cacacccatc aatgcctgac gtcccagatg tgctaatttc
    21181 atattcaccc cgaatgttgt tatgtctgtt tgcagtgtag agccattgtt tgtaaacaca
    21241 aaaacaactc cgctacatct tattcttatt taatatatat cagaagcagg caggctggag
    21301 gatttctgca caagtccggt acaggtttgg cgctgatagt gttgatactt ccgtaatgcg
    21361 atgcggtaat tgttggtgct ggtggtgggc agccacgggg cgaggatttc atcaaggaaa
    21421 ccgtcctgca gctgatcgag ggaaatattt tgctcatgta gatgattacc caaacggcgc
    21481 agacgaacga catattcacg caccgtacct gggctcattt cagtttgctc aaaaagaaat
    21541 ttcttaaagc cgataatatc gaaaaaatca ctctgcgttt tgcaatgtaa atcaccgcaa
    21601 aatcggcaca atgccgccca tgattgttgt tccgtttgcc agcctttttc atcaagcagc
    21661 gtgtccaggc gggaaatctc gattttattc actattttgc cgtcgcgaac taaggtaatg
    21721 cgatcgagta atttacggca atgcgcgcaa tgggtctggc tgtgtttaaa gtctttaaga
    21781 tagcggctta atggccgtct tttcaggtgc tgcaccatca ttaagaactc ctggtagtca
    21841 aagttaaagt gcggcattta ctgacgttat aacttaccca gtttagtgcg taaccgttta
    21901 atggcctggc tgtgtaactg actgacccgc gattccccga cctccagcac cgcgccaatc
    21961 tctttgagat tcagctcttc ctggtaatag agggttaata ccagtttttc gcgctccggc
    22021 aacgtttcga tggcttccat cacccgctgg cgcagattac tgtccagcag ttgttgtagc
    22081 gggttttctc gctgatgatc atcagtaacc agttcgatgc tatcgccgtg ctcttcacgc
    22141 cactcatcgt aggagaagag ctggctgtta ttggtgtcga gcagcatttg acgataatcg
    22201 gcaatatcga tccctaaacg ttccgctacc tcagtttccg tggcgttgcg gccaagttcc
    22261 tgctccagtt gccctattgc ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc
    22321 acccagtcac ggctgcgaag ttcatccagc atagcgccac ggatacgctg cactgcgtaa
    22381 gttgtaaatg ccgttccttg tagggcgtca tagcgttcga cagcattaag taacccaatg
    22441 ccgcccgcct gtagcagatc gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc
    22501 aatgcttcgt gacgcaccag cgggacataa cgctgccaca gcgagtgttt atccattaca
    22561 ccttcagcgg tatagagtga attcacgata aacagccctg cgttatatga gttatcggca
    22621 tgattatccg tttctgcagg gtttttaatc ggacgattag tgggtgaaat gaggggttat
    22681 ttgggggtta cgcaaaaagc cgaatgtaaa aaaccccgcc ggtggcgggg tttgagaggc
    22741 attcagacga ttaaccctgc agcagagaca gaacctgctg cggtacctgg ttggcttttg
    22801 ccagcacgga gttaccggcc tgctggataa tctgcgcttt cgacatgttg gacacttcgg
    22861 tcgcatagtc ggcgtcctga atacgggact gcgcttcaga caggttggta gtggtgttgt
    22921 tcaggttggt gactgcggaa tccaaacggt tttgcaccgc acccagggag gagcggaatt
    22981 tgtcgatgga gctgatggct tcatccagag ctttcagggg atcggctgtc gtagctgctt
    23041 ttgtttcagc atctgtcgtc aatttaccat cagcatcttt ataaactgcc ttaccgctgc
    23101 cgttagtcac tgaaccatca tcctgtaggt aaaaatctac agtatcagat gtggtcaacg
    23161 cctcgttgtt atcaacataa accgaatcaa caccttttgt tacagcgcca gttgtgccgt
    23221 tgacgctata acctgcagta gtgaaatcaa cattgtttcc cgaggttgat acagctttat
    23281 cgaaagctgc ctgatcaact tttgcacctg ttactgaata agtaatagtg tccggcgtag
    23341 tactattacc ggtaaaggag atagaggtac cgccaatgtc aatggttgtc gctttggtat
    23401 tattggctgc aagtgtggac agactagcct cagtcaaaga agcaccagag ccgtttttcg
    23461 ttaagcgccc agttgtatca atgtagagtt tatctccatt gcttgccgta attttaccat
    23521 cactgctgat ctgaacatcc tgtgcagaac catcgatagt aatagtgccc ttagcggtat
    23581 caccaactcc cggggtcaaa tatttctgaa catcggcagc tgaagccgtt gtggtatcaa
    23641 aagagtatga cttacttgca ctgtcatact tgtaattcgt tgcactagcc gccgttccga
    23701 agccgttatt aatggttgcc tgaacagtat caccatcact aactccagcc agcaaatcag
    23761 acgctttagc agcatcgtaa cccgcactca cagtatattt gttgccgacc actgttgcat
    23821 tagcagctac caagtcagct ttagatgcag cagtattagc cacagccccg ccaccattca
    23881 cattaaaccc attcagcccc agcgtatcag agtcaatttt cttcagatca atagtgatag
    23941 tctggccgtc attcgcacca acctgaattt tcatcgaacc gtcttttgcc agtacgttca
    24001 cgccgttgaa ctgggtctga ccggatacgc ggtcaatttc gtcgagacgg gatttgattt
    24061 cgtcctgaat ggagtccaga tccgaatcag agttagtccc ggtagaagcc tgaaccgtca
    24121 gctcacggat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgtg
    24181 caacagaaat accgtcgttg gcgttacgtg cagcctgagt caggccttta atgttagaag
    24241 taaaacggtt agcaatcgcc tgacccgcgg cgtcatcctt cgcgctgtta atacgcaagc
    24301 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    24361 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    24421 tgcaagtcgt tgattacgtt ttgggtttct acccgtcggc tcaatcgccg tcaaccctgt
    24481 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    24541 cgacgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    24601 aactttgcgc aattcaggcc gataaccccg gtattcgttt tacgtgtcga aagataaaag
    24661 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    24721 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    24781 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    24841 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc accaccagca
    24901 gcaccaccgc gttcagtgcc accaccgcgg gtaacgccat cgccgggaaa tacaccatca
    24961 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    25021 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcgac gacaaagatc
    25081 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgccatca
    25141 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    25201 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    25261 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    25321 tctcggtcgc cgcccagaat gcgcagctga cggtcaacaa cgtcgccatc gagaacagca
    25381 gcaacactat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    25441 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    25501 gggtgaatgc ctataactcg ctaatagata ccttcagcag cctgaccaaa tacaccgccg
    25561 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    25621 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    25681 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    25741 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    25801 ttggcgatgg taaaaaaacc ggcatcacaa ccaccatcgg cagcaacctg accagttggc
    25861 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaaaacc ctgaataagt
    25921 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaag
    25981 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    26041 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    26101 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc accgtaatga
    26161 gcgccagcca acagcagttg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    26221 gagcgcgact gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    26281 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaat
    26341 taacccaaaa cttaattgct ctttatagct atatggtcag gcgtttgctg caagccaatt
    26401 tacgcaacga tgtctccgca gtcgaagagg tggaagcatt aatgcgtaat attgccgatg
    26461 tctggaaaga gtctttactc tccccttctt cgattcagga cccagtctga tgaacaatgc
    26521 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    26581 ggcaacggaa gaacaatggg acgaactcat cgctagcgaa atggcgtatg tgaatgcggt
    26641 gcaggagatc gcacacctga ctgaagaggt tgccccgtcc actacgatgc aggagcagct
    26701 ccgcccgatg ctgcacctga ttctcgacaa cgaaagcaag gtaaagcagt tattacagat
    26761 tcggatggat gaactggcga aactggtcgg tcagtcatcg gtgcaaaaat cggtgttaag
    26821 tgcctatggc gatcagggcg gctttgtgct ggctccgcag gataacctct tttgaatctt
    26881 aatgagccga tggctcgcga ataatccgat tacggctacg cttctaatat tccccttgaa
    26941 tggagtcgaa gaatgcgtaa tcccacgctg ttacaatgtt ttcactggta ttacccggaa
    27001 ggcggtaagc tctggcctga actggccgag cgcgcagacg gttttaatga tattggtatc
    27061 aatatggtct ggttgccgcc cgcctataaa ggcgcatcgg gcggctattc ggtcggctac
    27121 gactcctatg atttatttga tctaggtgag tttgatcaga aaggcagcat ccctactaaa
    27181 tatggtgata aagcacaact gctggccgcc attgacgccc tgaaacgtaa tgacattgcg
    27241 gtgctgctgg acgtggtagt caaccacaaa atgggcgcgg atgaaaaaga agctattcgc
    27301 gtgcagcgtg taaatgctga tgaccgtacg caaattgacg aagaaatcat tgagtgtgaa
    27361 ggctggacgc gttacacctt ccccgctcgt gccgggcaat actcgcagtt tatctgggat
    27421 ttcaaatgtt ttagcggtat cgaccatatc gaacaccctg atgaagatgg catttttaaa
    27481 attgttaacg actataccgg agaaggctgg aacgatcagg ttgatgatga actcggtaat
    27541 ttcgattatc tgatgggtga gaatatcgat tttcgcaatc atgccgtgac ggaagagatt
    27601 aaatactggg cgcgctgggt gatggaacaa acgcaatgcg acggctttcg tcttgatgcg
    27661 gtcaaacata ttccggcatg gttttataaa gagtggatcg aacacgtaca ggaagttgcg
    27721 ccaaagccgc tgtttattgt ggctgaatac tggtcgcatg aagtcgataa actgcaaacg
    27781 tatattgatc aggtggaagg caaaaccatg ctgtttgatg cgccgctgca gatgaaattc
    27841 catgaagcat cgcgcatggg tcgcgactac gacatgacgc agatcttcac tgggacgctg
    27901 gtggaagccg atccttttca cgccgtaacc ctcgttgcca accacgacac ccaaccgttg
    27961 caagccctcg aagcaccggt cgaaccgtgg tttaaaccgc tggcgtatgc cttaattttg
    28021 ttgcgggaaa atggcgttcc ttcggtgttc tatccggacc tctacggtgc acattacgaa
    28081 gatgtcggtg gtgacgggca aacctatccg atagatatgc caataatcga acagcttgat
    28141 gagttaattc tcgcccgtca gcgtttcgcc cacggtgtac agacattatt tttcgaccat
    28201 ccgaactgca ttgcctttag ccgcagtggc accgacgaat atcccggctg cgtggtggtc
    28261 atgtcgaacg gagatgatgg cgaaaaaacc attcatctgg gagagaatta cggcaataaa
    28321 acctggcgtg attttctggg gaaccggcaa gagagcgtag tgacagacga aaacggcgaa
    28381 gcgaccttct tttgcaacgg cggcagcgtc agcgtgtggg ttatcgaaga ggtgatttaa
    28441 atccatcccc ggcggcaagc cggggagatt tcattacggc agtggcgtcg gtaacggcgc
    28501 tttatccagc gcggcggcac actctgccgt cgggcgatcc acacgctcca tcgtcatacc
    28561 atcgtactca atggtgttac cgtcacgctc aacctcgtac agctcacgtt ttacggtaac
    28621 gttagtgaga tcatcagaca tcaatgtcaa cttacccggc acggcaatcc cccgctgcca
    28681 ctgacggcaa tccaaggtat cgccctcttt agtgacaatc aggctgccta tcgcttccgg
    28741 actgaccagc gcacgttgcg gccctttggt ctgccagtag cccgccagcc agtccggcgc
    28801 gggggttttc acgacattgt tatagttttc gacttcggca cagcccgcca gcaacatgag
    28861 cgcgcctgca attgcgagtt ttttcatcat tcgtcctgca tgagaagaaa agaggattgt
    28921 ggcattaaag ctctgatgtc gccagccttt ccaggttcag agagaaattt gatctaacta
    28981 ttatttttgt gaattattac cccgaaaata aatttgcatg gtgtatgatt cgcggctctt
    29041 gtttcaaacc ttctgagttc agaggctaca ttcatgtcat ggcagcaatt caaacacgcc
    29101 tggttgatta aattctgggc acccatccct gcggtcatcg cggcgggtat tctctccact
    29161 tactattttg gcattactgg caccttttgg gctgtcacgg gtgaatttac ccgttggggc
    29221 ggtcagctcc tgcagctgtt tggcgtccat gctgaagagt ggggttattt taaaattatc
    29281 catctggaag gatcgccatt aacccgcatc gacggcatga tgatcctcgg tatgtttggc
    29341 ggctgctttg ctgctgcgct gtgggccaac aatgtcaaac tacgcatgcc acgcagccgt
    29401 atccgcatta tgcaggccat cattggcggc attatcgccg gttttggcgc gcgtctggca
    29461 atgggctgta acctggcggc gttctttacc ggcattcctc agttctcgct gcatgcctgg
    29521 ttctttgcca tcgccactgc cattggttca tggtttggcg cgcgctttac cctgctgccc
    29581 atcttccgta ttcccgtgaa aatgcagaaa gtttctgccg cctcaccgct gacgcaaaaa
    29641 ccggatcagg cgcggcgtcg ttttcgtctc gggatgctgg tctttttcgg catgctgggc
    29701 tgggcgctgc tcacggcgat gaaccaacca aaactggggc tggcaatgct gtttggcgtc
    29761 ggctttggtt tactgattga acgtgcgcaa atctgcttta cttcggcgtt ccgcgatatg
    29821 tggatcaccg gacgtaccca tatggcgaaa gcaatcatta tcggtatggc ggtgagtgcc
    29881 atcgggatct tcagttacgt acagttaggc gttgaaccta aaatcatgtg ggcgggacca
    29941 aatgcggtaa ttggtggttt actttttggt tttggcatcg tgctggcagg cggctgcgaa
    30001 accggctgga tgtaccgcgc ggtagaaggc caggtgcact actggtgggt cggtctgggc
    30061 aacgtgatcg gctcaacgat tctggcgtat tactgggatg atttcgctcc ggcgctggcc
    30121 accgactggg acaaaatcaa cctgctgaaa acttttggtc cgatgggagg cctgctggtg
    30181 acatatttgc tgctgtttgc tgcgctgatg ttgattatcg gctgggaaaa acgcttcttc
    30241 ccccgtgagg caccgcagac tgctaaggag atcgcatgaa aaatatcgtt cctgattacc
    30301 gccttgatat ggtgggtgaa ccctgccctt atccggcagt cgcaaccctt gaggcgatgc
    30361 cgcaattaaa aaaaggtgag atcctggaag tggtgagcga ctgtccgcag tcgatcaata
    30421 atattccact ggatgcgcgt aatcacggct acacggtgct ggatattcag caagacgggc
    30481 cgaccattcg ttatttaatt cagaagtaat ccctcattcc cgctgtggcc cacagcggga
    30541 acatcgttac ctctgacaca tagattttac ccgccccctc tcctattctt ttcagtatga
    30601 tctcaggagg ttgctatgtg tggacgcttt gcccaatccc aaacgcgtga ggattacctt
    30661 gcgcttctcg cggaagatat tgaacgcgat attccctacg atcccgaacc cattggcaga
    30721 tacaatgtcg cgccgggaac caaagttctt ctgctcagtg aacgtgatga acaccttcat
    30781 ctggatccgg ttttctgggg atatgctccc ggatggtggg ataaaccgcc gctgattaac
    30841 gcccgcgtag aaactgcctc aaccagtcgc atgtttaaac cgctctggca acatggtcgg
    30901 gcaatctgtt ttgccgatgg ctggtttgag tggaaaaaag aaggcgacaa aaaaagcctt
    30961 attttatcta tcgcgctgac ggacaacctg tttttatggc cgcgataggt agcataccgt
    31021 ttgagcgtgg tgacgaagcc gaaggatttt tgatcgtcac tgcggcggca gatcaaggtc
    31081 tggtagatat tcatgaccga cgcccgctgg ttctgtcgcc agaagctgca cgggaatgga
    31141 tgcggcaaga cattggtggt aaagaagcct cagaaatcgc taccagaagc tgtgtgccag
    31201 caaaccagtt cacctggcac cctgtgtcgc gcacggtggg taatgtcaaa aaccagggcg
    31261 cggagttaat tcaacctgtt tgttgacatc ctccacgccc tgaatgacga ggacccctgc
    31321 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    31381 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagctcca
    31441 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    31501 cgattttctt tcgctgtatg accacagcac gcgcaacgct ggcttgtgta cgctggcgga
    31561 acagcaagca cc
//
LOCUS       DADLCQ010000041        31306 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000041 DADLCQ010000000
VERSION     DADLCQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.041"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(435..641)
                     /locus_tag="JQG95_001702"
     CDS             complement(435..641)
                     /locus_tag="JQG95_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY5888286.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="JQG95_001703"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="JQG95_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY5888287.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="JQG95_001704"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="JQG95_001704"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY5888288.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="JQG95_001705"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="JQG95_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /locus_tag="JQG95_001706"
     CDS             4795..5367
                     /locus_tag="JQG95_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY5888289.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdbD"
                     /locus_tag="JQG95_001707"
                     /pseudo
     CDS             5360..8040
                     /gene="kdbD"
                     /locus_tag="JQG95_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8037..8714
                     /gene="kdpE"
                     /locus_tag="JQG95_001708"
     CDS             8037..8714
                     /gene="kdpE"
                     /locus_tag="JQG95_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY5888290.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSGVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8989..9093
                     /locus_tag="JQG95_001709"
     CDS             8989..9093
                     /locus_tag="JQG95_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY5888291.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9404..11601
                     /gene="speF"
                     /locus_tag="JQG95_001710"
                     /pseudo
     CDS             9404..11601
                     /gene="speF"
                     /locus_tag="JQG95_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11598..12917
                     /gene="potE"
                     /locus_tag="JQG95_001711"
     CDS             11598..12917
                     /gene="potE"
                     /locus_tag="JQG95_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY5888292.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="JQG95_001712"
     CDS             complement(12973..14613)
                     /gene="pgm"
                     /locus_tag="JQG95_001712"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY5888293.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="JQG95_001713"
     CDS             complement(14639..15184)
                     /gene="seqA"
                     /locus_tag="JQG95_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY5888294.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15369..16133
                     /gene="ybfF"
                     /locus_tag="JQG95_001714"
     CDS             15369..16133
                     /gene="ybfF"
                     /locus_tag="JQG95_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5888295.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16204..16566
                     /gene="ybfE"
                     /locus_tag="JQG95_001715"
     CDS             16204..16566
                     /gene="ybfE"
                     /locus_tag="JQG95_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY5888296.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            16706..17236
                     /gene="fldA"
                     /locus_tag="JQG95_001716"
     CDS             16706..17236
                     /gene="fldA"
                     /locus_tag="JQG95_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY5888297.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17446..17532
                     /gene="uof"
                     /locus_tag="JQG95_001717"
     CDS             17446..17532
                     /gene="uof"
                     /locus_tag="JQG95_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY5888298.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17525..17971
                     /gene="fur"
                     /locus_tag="JQG95_001718"
     CDS             17525..17971
                     /gene="fur"
                     /locus_tag="JQG95_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY5888299.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18055..18381)
                     /locus_tag="JQG95_001719"
     CDS             complement(18055..18381)
                     /locus_tag="JQG95_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888300.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="JQG95_001720"
     CDS             complement(18418..19824)
                     /gene="chiP"
                     /locus_tag="JQG95_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY5888301.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="JQG95_001721"
     CDS             complement(20403..22067)
                     /gene="glnS"
                     /locus_tag="JQG95_001721"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY5888302.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="JQG95_001722"
     CDS             complement(22270..24216)
                     /gene="nagE"
                     /locus_tag="JQG95_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY5888303.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24549..25349
                     /gene="nagB"
                     /locus_tag="JQG95_001723"
     CDS             24549..25349
                     /gene="nagB"
                     /locus_tag="JQG95_001723"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY5888304.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25409..26557
                     /gene="nagA"
                     /locus_tag="JQG95_001724"
     CDS             25409..26557
                     /gene="nagA"
                     /locus_tag="JQG95_001724"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY5888305.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26566..27786
                     /gene="nagC"
                     /locus_tag="JQG95_001725"
                     /gene_synonym="nagR"
     CDS             26566..27786
                     /gene="nagC"
                     /locus_tag="JQG95_001725"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY5888306.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27834..28586
                     /gene="nagD"
                     /locus_tag="JQG95_001726"
     CDS             27834..28586
                     /gene="nagD"
                     /locus_tag="JQG95_001726"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY5888307.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28983..30647
                     /gene="asnB"
                     /locus_tag="JQG95_001727"
     CDS             28983..30647
                     /gene="asnB"
                     /locus_tag="JQG95_001727"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY5888308.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31026..31102
                     /locus_tag="JQG95_001728"
     tRNA            31026..31102
                     /locus_tag="JQG95_001728"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31060..31062,aa:Met,seq:cat)
     gene            31111..31195
                     /locus_tag="JQG95_001729"
     tRNA            31111..31195
                     /locus_tag="JQG95_001729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31145..31147,aa:Leu,seq:tag)
     gene            31219..31293
                     /locus_tag="JQG95_001730"
     tRNA            31219..31293
                     /locus_tag="JQG95_001730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31251..31253,aa:Gln,seq:ttg)
BASE COUNT         7462 a         7904 c         8153 g         7787 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggcgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggagttacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ctactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ccgccgcgcc cgatccgctg gtgaaatttt ccggtgttac cgtcgattta gccgcccgcg
     8461 tgattcaccg gggtgaggaa gaggtgcatc tcacaccaat tgagttccgc ctgctggcgg
     8521 tgctgctcaa caatgccgga aaagtactca cccagcgcca gctccttaac caggtgtggg
     8581 ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat ctgcgacaaa
     8641 aactggaaca ggatccggcc cggcctcgcc acttcattac tgaaaccggt attgggtatc
     8701 ggtttatgct ttgaattgtt attcaataaa ttcgcttaaa tagtgattaa tttattatta
     8761 aacctgcgcc gaatattatt atttggcagg tttaatttct taacgcaaat ataaaacacg
     8821 aaattacaac atttaagtaa caaaacagca tctaatatgg cgatattcat aattaatgat
     8881 ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata gccatttcat
     8941 ggatagaata actctacctt caactgacac agcaagcggt aaaggtaaat ggaaaataat
     9001 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
     9061 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc accccaacgt
     9121 acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg tgctcatata
     9181 ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact tgaggtatga
     9241 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
     9301 atttatttat cagcggtgga gctttgcttt ttttccgacg tgatcgattt ctcctttgag
     9361 aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag aattaaaaat
     9421 tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg tgaatattga
     9481 cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg ttgaacgtgg
     9541 aaaactcgat aaaatagacg ctactggcta tggcattcct gtttttattg caacggaaaa
     9601 tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg agcattgcga
     9661 atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt atgaaacgca
     9721 actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca atagcgcttt
     9781 tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag ggaatcagtt
     9841 cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag acgtagcaat
     9901 gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg cggcaaaagt
     9961 gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta acaaagtggt
    10021 tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata accacaaatc
    10081 taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg aaacggcacg
    10141 taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa gttacctgcg
    10201 tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt tccgcctcgc
    10261 tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg tggtggataa
    10321 aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct atgagcaatt
    10381 tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga acgatccggg
    10441 tattctagtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa cttcacaaat
    10501 ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca aacgcatgaa
    10561 caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg ccgcactgga
    10621 tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg attgtgtggt
    10681 aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc gtccgttcgt
    10741 acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa ttgcggttga
    10801 tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag gctatgcaga
    10861 gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta ttgatgcacg
    10921 taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact tcctgcgcga
    10981 aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc tgactccggc
    11041 agaagatatg gccaaacttc agcaacttgt gccctgctgg tacgcttcga aaaactgctt
    11101 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
    11161 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgccac
    11221 aacgtgaaac aactgcaaaa agagatgttc cgcaaagcgc acttcccaca cgtcagcatg
    11281 aatccgcaag aagccaacta cgcctattta cgcggtgaag tggagctggt tcgtctgccg
    11341 gatgccgaag gtcgtatcgc tgccgaaggg gcgcttcctt atcctccggg tgtgctgtgt
    11401 gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
    11461 gggatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
    11521 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
    11581 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc gttcagttaa
    11641 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
    11701 ccgaagttgg gacaatttca attatctcct ggctggtgac agccgtaggc tcaatggcac
    11761 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggtggt atgggcggtt
    11821 atgccgagta tgctttcggt aaatccggta actttattgc gaactatacc tacggcgtct
    11881 cactgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
    11941 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
    12001 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
    12061 gggggggcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
    12121 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgca gtaggttctt
    12181 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
    12241 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
    12301 cggtgattta tatcgtctcc accaacgtga ttgcagggat tgtgcctaat atggagctgg
    12361 caaattcaac ggcaccattt ggcctggcct tcgcacagat gtttacacca gaagtgggta
    12421 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
    12481 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
    12541 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
    12601 ggttggcgct gatgacgatt agcccgtcgc taaacagtca gttcaacgtg ctggttaacc
    12661 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
    12721 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
    12781 gcgcgatgta tagcttttat gcgctgtact catccgggga agaagccatg ctgtacggtt
    12841 ccatcgtgac cttccttggt tggacactgt atggtctggt atcaccacgc tttgaactga
    12901 aaaataaaca cggttaaaaa agcaaagtaa aaaagggcga tcttgcgacc gctctttttt
    12961 tattaaatgt gtttacgcgt ttttcagaac ttcgctaaca atctcaaccg cttctttctc
    13021 aatctgcttg cgatgttctt caccgaggaa gctttcgcag tagatcttat atgcgtcttc
    13081 cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc atcactttca gaccgccaat
    13141 agaagcaccg ttgcccggag cagcagtcag gcgcgcggtg atcgggtcac ctgccagggt
    13201 gctggcgctc accatttccg gagacagctt agacagcgcc gctttttgtg cggaagtcgc
    13261 agctgcctgc aaacggttgt agctcggcgc accaaagcgt tttgccagtt cgttgtagtg
    13321 ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc gccagcagac acataatgat
    13381 gccgtctttg tcggtggacc acggcgtgcc gtcgaaacgc aggaaggaag cccctgcgct
    13441 ctcttcgccg ccaaagccga agctgccgtc gaacagacca tcaacaaacc atttgaaacc
    13501 taccggtact tctaccagct tacggcccaa gtcattgacc acacggtcga tcatcgcaga
    13561 agaaaccagc gttttaccga cagcaacatc tttgccccac tgcggacgat gctggaacag
    13621 gtagttgatc gccaccgcca ggtagtggtt cggattcatc aaacctgccg gagtgacgat
    13681 accgtgacgg tcataatccg ggtcgttagc aaacgccaga tcgaacttat cacgcagtgc
    13741 cagcagaccc gccatcgcac actcggagga gcagtccata cggatcgcac cgtctttatc
    13801 gaggtgcata aagcggaagg tttgatcgac ctgatcgtta acgatggtca ggttgaggtt
    13861 gtaatactcg ccaatgcgct tccagtattc gataccggaa ccgcccaacg gatcaacgcc
    13921 cagcgtcagg cccgctttct gaatcgccgc catatcaacg atatcggcca gcccttccac
    13981 gaacggctgc accagatcct gctctttcac atagccggat gccatcgctt cgtcgaggga
    14041 gatacgcttc acgcctttca ggccatcggc catcagtgcg ttggccctgt cttccaccac
    14101 tttggtgacg ttagtatcag ccgggccacc atttggcgga ttgtacttga taccaccatc
    14161 ttccggcggg ttatgggacg gtgtaatcac gataccgtct gccagcgggc cacctttttt
    14221 attgtgaacc aggatggcat tggaaacggc aggcgtcgga gtgaagccat tgttttcctg
    14281 cacaatgaca tcaacgccgt tcgctgccag cacttccagt acggaaatga acgcaggctc
    14341 ggacagggcg tgagtatctt tacccacata gcaagggcca gtgatgccgt ttttcgcacg
    14401 ttcttcagca attgcctgag cgatcgccag aatgtgcggc tcgttaaagc tgtggcgcgc
    14461 tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg tgctccgcat tccctgcttc
    14521 tggtttcagt acataatatt gcgccgtcag ttgggcgacg ttaatcaaat cactctgttg
    14581 tgcaggttgg cctgcacgat tgtggattgc cattgctttg tcctttgtct gcaacgtttt
    14641 agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa ttgcatcgac tgcatgatgt
    14701 gttcgatcat gctgcattta cggccggtgt tggtgttggt gatcacccaa tacggcgtgc
    14761 ctggcacatg tttcggcttg gtctgattac catttttcag cagcgtttgt tcatctgccg
    14821 caaagtacac gcgtgtacga ccgtgcaacg attccgttgc ttcggcaaac gcctgggcgt
    14881 caagagaata tagtgtagac aacagcagca taaagcgatt gaccgctcgc ttttgctctg
    14941 cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac cttgtcttta atcgttttga
    15001 ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac ctctttcgtc accggagcag
    15061 caggctgtga tgcggcggaa aatttcaaca tacgccgtaa aatgtcggat gcgctctcgc
    15121 cgatatgctt agtgtggctg gcaatatagc tgtagagttc atcatcaact tcaatcgttt
    15181 tcatcttaat ccagtgcagt gtcttagctg aatacaagtc gccaagagta tatgaataaa
    15241 tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa ctcggaataa accttatttg
    15301 ttgcagccct gtggcacaat ggtcaacata ataccctaac ccgacaacgc gaaaaaaaga
    15361 actttgccat gaaattaaat atccgcgcgc aaactgcaca aaaccagcac aataattctc
    15421 ccatcgttct tgtccatggt ctgtttggta gcctcgacaa ccttggcgta ctggctcgcg
    15481 atctggtaaa cgatcacaat atcatccagg ttgatatgcg taaccacggt ctttcaccga
    15541 gagagccggt aatgaattac ccggcgatgg cgcaggatct tgttgatacc ctggatgcac
    15601 tgcagatcga caaagcaaca tttatcggtc actccatggg cggtaaagcg gtaatggcac
    15661 ttactgcact ggcccccgat cgcatcgata aactggtggc gatcgatatc gcaccggtcg
    15721 actatcacgt acgccgtcat gatgagattt tcgcagctat caacgcggtc agtgaatcgg
    15781 acgcacaaac tcgccagcaa gcagcagcaa taatgcgcca gcatcttaat gaagaagggg
    15841 tgattcagtt tctgttgaaa tcttttgttg acggggagtg gcgctttaac gtgccggtat
    15901 tgtgggatca gtatccgcat attgtgggtt gggagaaaat cccggcatgg gatcaccctg
    15961 ccctgtttat ccctggcggc aattctccgt atgttagcga gcagtaccgt gatgatttac
    16021 tggcacaatt tccacaggca cgggcgcatg tgattgcagg cgcgggtcac tgggtccatg
    16081 ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta tctcaatgat taacgcaact
    16141 gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtggg cgttggcgtg actcatcgcg
    16201 ctgatgtatt atggcgcgct atccatccgg gcagaggcct ggctgattgt ttcccccgaa
    16261 gtcaccaaga tcatggccaa agaacaaacg gaccgtacga cattagatct gttcgcgcac
    16321 gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc gcgatgaaca gctgcgtatt
    16381 aataaacgca accagctaaa acgcgacaaa gtacgtggcc ttaagcgtgt cgaactgaag
    16441 ctgaacgcgg aagctgtcga ggcgctgaac gagctggcgg aatcgcgtaa tatgagccgt
    16501 agcgaactga tcgaagagat gttaatgcag caactggcgg cgctgcgtag tcagggcatc
    16561 gtttaaattt ccactttcat gtagcacagt gtgcagtcct gctcgtttgc tgattccgca
    16621 agactgcctg ttctgctatg attgccttta tccgtgggca attttccacc cccatttcaa
    16681 taagtttcaa gaggttattt cactcatggc tatcactggc atctttttcg gcagcgacac
    16741 cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag cttggtaaag acgttgccga
    16801 tgtccatgac attgcaaaaa gcagcaaaga agatctggaa gcttacgaca ttctgctgct
    16861 gggcatccca acctggtatt acggcgaagc gcagtgtgac tgggatgact tcttcccgac
    16921 tctcgaagag attgatttca atggtaaact ggttgcgctg tttggttgtg gtgaccagga
    16981 agattacgcc gaatatttct gcgacgcatt gggcaccatc cgcgacatca ttgaaccgcg
    17041 cggtgcaacc atcgttggtc actggccaac tgcgggctat catttcgaag catcaaaagg
    17101 tctggcagat gacgaccact ttgtcggtct ggctatcgac gaagaccgtc agccggaact
    17161 gaccgctgaa cgtgtagaaa aatgggttaa acagatttct gaagagttgc atctcgacga
    17221 aattcttaat gcctgatgtg atgcggcgta gactcatgtc tacgccgtat taatagataa
    17281 tgccaatcaa aataattgct acaaatttgt aacttttgct gttgtacctg tacaatgtcc
    17341 cagtgttcaa gtggccttgc cgttgtaaat gtaagctgtg ccacgttttt attaacaata
    17401 tttgccaggg acttgtggtt ttcatttagg cgtggcaatt ctataatgat acgcattatc
    17461 tcaagagcaa attctgtcac ttcttctaat gaagtgaacc gcttagtaac aggacagatt
    17521 ccgcatgact gataacaata ccgccctaaa gaaagctggc ctgaaagtaa cgcttcctcg
    17581 tttaaaaatc ctggaagttc ttcaggagcc ggacaaccat cacgtcagtg cggaagattt
    17641 atacaaacgt ctgatcgata tgggtgaaga aattggtctg gctacggtat atcgcgtact
    17701 gaaccagttt gacgacgctg gtatcgtcac ccgccacaat tttgaaggcg gtaaatccgt
    17761 atttgaactg acacagcaac atcaccacga tcacctgatt tgcctcgact gcggcaaggt
    17821 tatcgaattt agtgatgatt ccatcgaagc gcgtcagcgt gaaattgccg caaaacatgg
    17881 cattcgcctg actaaccaca gtctctatct ttacggtcac tgtgccgaag gcgattgccg
    17941 cgaagatgag cacgcgcacg aaggcaaata agccagcctg aacgagaaaa gccaacctgc
    18001 gggttggctt ttttatacaa gggaaataag aaaagcgaga gttacagctc tcacttattt
    18061 gttattactg cgtatttcct gccagacctt atcacaatcg gctttcaccg cctgatcatt
    18121 tccggtttgc gttgcacgca gacactgctg ataatccagt acgcgtacac tttcctggtc
    18181 ggcaaactgc tgatgttgtt tctctttctt cagcacgttt aacacgctct ggcaggcttc
    18241 aattttttct ggcgaacctt gcgcggtgtt gatacaggcg ctgtatgcct ctttcagacg
    18301 gctgtcttcc tgtggtgcgg ttgattgcgc acaagctacc agccccgatg ccatcatggc
    18361 gatgagaatc agttttttca ttgaatgcgg caggcacaaa acctgccgcg tcgggcatca
    18421 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
    18481 accgtaaccg ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaagtgcag
    18541 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
    18601 ggcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
    18661 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
    18721 ccagtttttc aggtcataca tcgcactgaa gaagaccgct ttttcgccgt tagcgttgaa
    18781 gtcggaacgg ttatcccacc agatatccag gcggccgttg gaggaagcgt aggttggggt
    18841 catacgttgc aggaagtatc cctgctgact gtcagcctta atccaggtgc cttcgaggcg
    18901 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcttgcagcc aggcggtgcc
    18961 gtcataaaga tcgttgacgc tgcgatcgtc aactttatcg cgggtaccat agaactggta
    19021 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    19081 atcgatatac ccttccgcct gaccaaatgc cgcttccagt acgaagttat ttttgaagtc
    19141 gtatttcgca ccgatggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    19201 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    19261 caaggcacca gcatcgccgt aatcaaaatt cgccccggct tccgccccct gataagtacc
    19321 cggcataaag ctccagtgcg gcgctaacag cgtttgaccg gttggctgaa tgtaacctgc
    19381 cctcgcccaa accggaccgt atttaaattt ggccgcagct ttatacaggc ttataccgct
    19441 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    19501 cgggtgggag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcag caatatcaag
    19561 gccgaacata tcagcagcat aaccggactg aaaatcgagg ttggcattcc aggtggagtg
    19621 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    19681 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    19741 cggcattgcc ataaagcccg acattgctgt aacaccggca atagccagcg ccagcgtact
    19801 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    19861 aaggtgaaca gcgaaaaaaa ataaaaatta aaaaagtagc gttgagaaaa gtctcgctga
    19921 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    19981 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    20041 ttgaaaggct tgcagaagaa taaaaacgtg aaattccagc aagatgaaag cggttacatt
    20101 ttatttaatt atgttttgtg attttaaatt tattttatgg ataaattgcg aagcggaatt
    20161 aattcgcgaa gaaaagcaga aaaaacgccg cagagcgcgg cgtttgggat tgtttgctat
    20221 ttcttgccgg atgcggcgtg aacgccttat ccggcctaca aaagcacgca aattcaatat
    20281 attgcagaga tcatgtaggc ctgataagcg aagcgcatca ggcaatttag cgtttgaaat
    20341 ttattgtcta tttattgccg gatgcgacgt aaatgcctca cccggcttgg tagaacttaa
    20401 aattactcgc ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt
    20461 ttttcggccg tggaatggcg gctgtcgagg cagaagtaac cttcacgctc aaactggaat
    20521 gctttacccg caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc
    20581 gattccgggt taatcaccga caggaaatca tccgcagcac ctgggttagg cacgctgaac
    20641 agacgatcat acaaacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata
    20701 acacctttga ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta
    20761 cagaagatgg tggtgatatt accttcggca tctttctcca cgcgttcagc cttaatgacg
    20821 taagcattac gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct
    20881 tcttcgcgga aatcggcgcg atcgatccaa atttcaccgc taaacggcac ctgacggctc
    20941 cccatttccg gtttgttcgg atggttcggc atggtgacca tttcgccttc gccctgatag
    21001 ttttcgataa ccagtttcac cggatcgata accgccattg cgcgcggcgc attttcgttg
    21061 agatcttcac ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc
    21121 acgccgatgc gtttgcagaa ctcacgaata gaagccgcag tgtaaccacg acgacgcaga
    21181 ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc
    21241 aggttcagct tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac
    21301 tggcgcggat gaacaggaat ggtgatgtta tccagtaccc agtcgtacag acgacggttg
    21361 tcctggaact caagcgtaca cagagagtgc gtaatacctt ccagcgcatc gctgatgcaa
    21421 tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca
    21481 aatttaatgc ggtacagcac cggatcgcgc attacgataa atggcgacgc catgtcgatt
    21541 ttcgcacgca ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc
    21601 gccaagttct cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ctgcgtcagg
    21661 gtaccgcggt attcgcggat ctgttccggt gtcagttcat caacgtacgc caggccttta
    21721 ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg
    21781 ttaccagacc agtgaaaacc taaccactct acgtcgtttt tgatcgaatc gacatactcg
    21841 atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc
    21901 tgggcgatcc cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc
    21961 ggctccggcg ggaaacgggt gtgtactgtg atgtgcttac cactggccag atcttcatcg
    22021 atgatctgac ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca
    22081 aagcgtaaac aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta
    22141 gttttttaaa attgataatc taacaaggat tatggggatg attgatgaaa aaaaaagcgg
    22201 cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctgccgtgtc gcatcaggca
    22261 taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa catggccctg
    22321 agctttaatg agcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat
    22381 cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct
    22441 tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc
    22501 gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga acgcgtggtt
    22561 ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg
    22621 tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc
    22681 cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg
    22741 tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag ctgacgcagc
    22801 ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt ctgctttcgc
    22861 gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag cacccagacg
    22921 tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca gacgggtaat
    22981 acaggcatca atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc
    23041 cagttgagtc agaccttctt cagtattgct gttggcttct tcagtaacga tctcgtcttc
    23101 tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac
    23161 gaagtagata gcgaagaaga taacgcccat caccagcagc atccagacgt tctggctggc
    23221 ggcaggcagg ttatacatca acgcgtagtc gatagcaccc gcagagaaag agaagccggc
    23281 atggataccc agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag
    23341 gtacagcagc ggcgcaagga acatgaacag gaattccagc ggctcagtca caccggtcag
    23401 gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg
    23461 tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa
    23521 gccggacatg aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg
    23581 gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag
    23641 cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc
    23701 cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg
    23761 ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg cgatcggcac
    23821 aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt
    23881 ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc
    23941 tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc
    24001 cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc
    24061 gaggttatca aaaattgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg
    24121 ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga gctgtaacgc
    24181 cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta
    24241 tttggctcgt ttaaagccgt atttttattt tgctgcaaat tttactgccg atgttctgta
    24301 atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgca tcgcaaatta
    24361 aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg
    24421 ctaaaccaga aaacttattt tatcattcaa aaaatcaagt cggattgacg cctgtctgcg
    24481 caaatccagg ttacgcttaa agatgtctaa tccgccaacg gcttacattt tacttattga
    24541 ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc
    24601 gccatatcgt caatcgtatc aatgcgttca aaccaactgc cgatcgtccg tttgtactgg
    24661 gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag
    24721 caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc
    24781 cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg
    24841 atattccagc agaaaacatc aaccttctca acggcaatgc cccggatatc gatgccgagt
    24901 gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg
    24961 taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc
    25021 gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg
    25081 tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacactgctg gatgccgaag
    25141 aagtgatgat tatggtgctg ggtagccaga aagcactggc gctgcaggcc gccgttgaag
    25201 gttgcgtgaa ccatatgtgg accatcagct gtctgcaact gcatccgaaa gcgatcatgg
    25261 tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat
    25321 tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggttc cttgctcagg
    25381 gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc ggatctttac
    25441 cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt
    25501 ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct
    25561 ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac
    25621 cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg
    25681 ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt
    25741 gcgcgttatg cgtgagtacc tggcaaaaca tccgaatcag gcgttaggtc tgcatctgga
    25801 aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc
    25861 tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct
    25921 ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt
    25981 ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc gcgcggggat
    26041 tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct
    26101 ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca
    26161 tgttgattac gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt
    26221 taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac
    26281 aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt
    26341 aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt
    26401 gctgcgtatg gcgacgctct atccggcgcg tgcgattgac gttgaaaaac gtctcggcac
    26461 gctcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta aaatcaccaa
    26521 gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga
    26581 caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc
    26641 ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc
    26701 cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt
    26761 gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat
    26821 ttccacgcaa tcggcgtacg gcttggtcgt catgacgcca ccatcactct gtttgatctc
    26881 agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca gcagacgctg
    26941 gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg caagctgcgc
    27001 gagctaatcg ccatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt
    27061 cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact ggaagaacgt
    27121 tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac
    27181 ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg
    27241 gccgggatta tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt
    27301 ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg
    27361 gaaactatcg ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc
    27421 taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa agccgcgaac
    27481 aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct gggtaaaacc
    27541 atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg tgaaatcacc
    27601 gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca agcgctgaag
    27661 gcgtttcgta ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc
    27721 gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa
    27781 aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca
    27841 ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg
    27901 gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca
    27961 actatccttc gcagactggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg
    28021 tgccggatag cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg
    28081 aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg
    28141 gtttcactat taccgatgtg aaccctgatt tcgtgattgt tggcgaaaca cgttcctaca
    28201 actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca
    28261 ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga
    28321 ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc
    28381 gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac agtgattgtc ggcgataacc
    28441 tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg gtgctttctg
    28501 gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt
    28561 cagtcgctga aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg
    28621 atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat
    28681 atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    28741 caaatcgcac tttatcagca atttggcccc cgtaaaatat aaccattcaa taaaacccgc
    28801 taaaaattat cgattcatca ataaatacat cctcacacca gacccttttt caccaatcga
    28861 caatcaccat tacgtttttt atttttgcac tgccaaatag cttgcgcaca gtgctgcttt
    28921 gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg
    28981 ttatgtgttc aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga
    29041 aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg
    29101 ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt aacgcggggg
    29161 cgcaacctct ctacaaccaa caaaaaaacc acgtactggc ggtaaacggt gaaatctaca
    29221 accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca gggtctgact
    29281 gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg
    29341 gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt ggtcgcgacc
    29401 atctggggat catcccactg tatatggggt atgacgaaca cggtcagctg tatgtggcct
    29461 cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct
    29521 atttgtggag tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg
    29581 atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg
    29641 ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct actttctggt ggtctggatt
    29701 cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa gatcaggaac
    29761 gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg ggttcaccgg
    29821 atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact
    29881 tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacttatg
    29941 atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcga
    30001 tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt
    30061 acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt aaactgctgg
    30121 ccttgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag
    30181 cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg
    30241 ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat
    30301 acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca
    30361 gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa
    30421 ctgcccgctt ccgcttcccg tacaacacgc cgacctcaaa agaagcgtat ctgtaccgcg
    30481 agatctttga agaactgttc ccacttccga gtgccgctga gtgcgtgccg ggcggtcctt
    30541 ccgtcgcgtg ttcttcagct aaagcgatcg aatgggatga agcgttcaaa aaaatggacg
    30601 atccgtctgg tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcggtact
    30661 gccccggaga aatccggggc tgttttagtt attgcgctgc ttgttgattc tgatatgcag
    30721 taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta
    30781 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa
    30841 agaatggctg tagggtgggg aaaaacacaa agtaaacaat aattgacgaa tatagcgcca
    30901 cgctgttcgc aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg
    30961 acgctgcaag gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa
    31021 aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    31081 tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt agacgcacca
    31141 gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac
    31201 cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt ttgataccgg
    31261 cattccctgg ttcgaatcca ggtaccccag ccatcgaaga aataat
//
LOCUS       DADLCQ010000042        31186 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000042 DADLCQ010000000
VERSION     DADLCQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.042"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            389..2047
                     /locus_tag="JQG95_001731"
     CDS             389..2047
                     /locus_tag="JQG95_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY5888309.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="JQG95_001732"
     CDS             complement(2044..3000)
                     /locus_tag="JQG95_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY5888310.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="JQG95_001733"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="JQG95_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY5888311.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="JQG95_001734"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="JQG95_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY5888312.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQG95_001735"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="JQG95_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY5888313.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="JQG95_001736"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="JQG95_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY5888314.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="JQG95_001737"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="JQG95_001737"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY5888315.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="JQG95_001738"
     tRNA            complement(9859..9946)
                     /locus_tag="JQG95_001738"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JQG95_001739"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="JQG95_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY5888316.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JQG95_001740"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="JQG95_001740"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY5888317.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JQG95_001741"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="JQG95_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY5888318.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JQG95_001742"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="JQG95_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY5888319.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JQG95_001743"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="JQG95_001743"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY5888320.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNA
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="JQG95_001744"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="JQG95_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY5888321.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="JQG95_001745"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="JQG95_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY5888322.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANGGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFVQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="JQG95_001746"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="JQG95_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY5888323.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /locus_tag="JQG95_001747"
     CDS             22003..23346
                     /locus_tag="JQG95_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY5888324.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLIEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="JQG95_001748"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="JQG95_001748"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY5888325.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMIAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27413
                     /gene="dmsA"
                     /locus_tag="JQG95_001749"
                     /pseudo
     CDS             24969..27413
                     /gene="dmsA"
                     /locus_tag="JQG95_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27424..28041
                     /gene="dmsB"
                     /locus_tag="JQG95_001750"
     CDS             27424..28041
                     /gene="dmsB"
                     /locus_tag="JQG95_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY5888326.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28043..28834
                     /locus_tag="JQG95_001751"
     CDS             28043..28834
                     /locus_tag="JQG95_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY5888327.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28870..29496)
                     /locus_tag="JQG95_001752"
     CDS             complement(28870..29496)
                     /locus_tag="JQG95_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY5888328.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29811..30959
                     /locus_tag="JQG95_001753"
     CDS             29811..30959
                     /locus_tag="JQG95_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888329.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7642 c         8378 g         7480 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt attgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 gcgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ttgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct agtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacggtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatgg cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgtg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagccgcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgatcgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgatcg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctaccgat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc
    25261 tacactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcaccgtgtc tacaatccgg
    25321 accgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca
    25381 ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt
    25441 acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc
    25501 gctcctggcc gccgggaaat accctggtcg cgcgcctgat gaactgctgc ggcggttatc
    25561 ttaaccatta cggtgactac tcttccgcgc aaattgcgga aggtttgaac catacctacg
    25621 gcggctgggc agatggtaac agcccgtcgg atatcgaaaa cagtaaattg gtagtgctgt
    25681 ttggtaataa cccaggcgaa acgcgaatga gtggcggtgg ggtgacttac tatcttgaac
    25741 aggcacgcca gaaatctaat gctcgcatga tcatcatcga tccgcgctat accgacaccg
    25801 gtgccgggcg cgaagatgag tggatcccta ttcgtccagg aacagatgcc gcactggtta
    25861 acggtctggc gtacgtcatg atcactaaaa acctggtgga tcaggcattc ctcgataaat
    25921 attgcgttgg ctatgacgag aaaaccctgc ccgccagtgc gccgaaaaat ggtcattata
    25981 aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcgc
    26041 aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca
    26101 aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa
    26161 cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaat ggagggaaca
    26221 gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc
    26281 cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa
    26341 tgacggcgct gcgtgatggt gtgcgcggga aagataagct ggatgtgccg atcaaaatga
    26401 tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg
    26461 aaatcctcca ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct
    26521 catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact
    26581 ttgcactgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta
    26641 aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg
    26701 gcgttgagca acagtttact gaaggccgta cctaggaaga gtggatgcgg catctgtatg
    26761 cccagtcgcg ggaagcgatt cctgaactgc caacgtttga agagttccgc aagcagggga
    26821 tctttaaaaa gcgcgaccca caagggcatc acgttgctta taaagccttc cgtgaagatc
    26881 cgcaggcaaa cccactgact acgccatcgg gcaaaattga gatttattcg caggcgctgg
    26941 ctgacattgc cgctacctgg gaattgccag aaggcgatgt gatcgatcca ctgccgatct
    27001 acacgccggg ctttgaaagt tatcaggatc cgctgaacaa acagtatccg ctgcagctta
    27061 caggtttcca ctataaatct cgcgttcact caacttacag caacgttgat gtgctgaaag
    27121 cggcttgccg tcaggaaatg tggatcaacc cgcttgatgc ccaaaaacgc ggtatccaca
    27181 acggcgataa agtcaggatc tttaacgatc gtggtgaggt tcatattgag gcgaaagtga
    27241 cgccacgaat gatgccgggc gtggtcgcac tgggtgaagg cgcctggtat gacccggatg
    27301 caaaacgtgt cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc
    27361 tcgctaaggg gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa
    27421 ccgatgacaa cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc
    27481 tgcgagctgg cttgtaaaga ctacaaagat ttgacgccag aagtcagttt ccgccgcatt
    27541 tatgaatatg ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt
    27601 gcctactatc tgtcgatttc gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc
    27661 ccgagcggtg cgatgcataa acgcgaagat ggttttgttg tggtcgatga agatgtatgc
    27721 attggctgcc gttactgtca tatggcttgc ccgtatggcg caccgcaata taacgaaacg
    27781 aaaggccata tgaccaaatg cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg
    27841 atctgtgttg aatcttgccc gctgcgggcg ctggatttcg gccctatcga cgagctgcgt
    27901 aaaaaacatg gcgatctggc ggccgttgcg ccgttgccgc gagctcactt taccaaaccg
    27961 aatattgtga tcaaacccaa tgccaatagc cgcccgaccg gggataccac tggctatctg
    28021 gcaaacccga aggaggtgtg agatgggaag tggatggcat gaatggccgc tgatgatctt
    28081 cacggtcttc gggcaatgtg tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa
    28141 aggcgacctg cgagcagaag cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt
    28201 actgatgggc attggcttta tcgcctctat gctccatctt ggttcaccga tgcgcgcctt
    28261 taactcgctc aaccgggtag gggcttcagc actcagtaac gaaatcgcca gcggttcgat
    28321 cttttttgct gtaggcggca tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc
    28381 attgcgtacg ctatggctgg tggtgacgat ggttcttggc gtcgtctttg tctggatgat
    28441 ggtgcgtgtg tataacagca ttgataccgt tccgacctgg tacagcatct ggacgccgat
    28501 gggctgggcg atgcgtctgc tgccagccat ttctgtactg gcactggtgg tgagtggcgt
    28561 gatgtcagtg atgcagggca cagagctggc aactattcat agttctgtgc agcaggccgc
    28621 agcgctggtg ccggactatg gtgcgctgat gtcctggcgg atcgtgcttt tggccgttgc
    28681 cctgtgcttg tggattgcac cacagctaaa aggttatcag cctgcggttc cgctactatc
    28741 cgtctcattc attctgcttc tggcagggga attaatcggt cgcggcgtat tctatggttt
    28801 gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta acaaccgggg tttcggcccc
    28861 ggttctcttt tatttctgct tcgttaacgt gtcataactg gtcatcaaat tacgataatc
    28921 aggaatatgg ttagagaaca acgtcgccag tccttcaata tcattacgcc agtcacggtg
    28981 cagctcgcaa gccacgccga accatgtcat caattgcgca ccagcttgcg acatgcgatc
    29041 ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat gcatcagtca cgacaaagac
    29101 atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc acgcaaactt cggttaccac
    29161 accggcaata attaactgtt ttttacctgt cgctttgact gcttttacaa aatcttcgtt
    29221 atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt gcatcgggga attgtgcttt
    29281 cagttctaga actaatgggc cgttaggacc ggtttcaaaa ctggtggtaa gaatagttgg
    29341 taagttgaaa tacttagcca ggtcacccag cgccagcacg ttatttttaa acttatcggg
    29401 ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga tcaacaagca aaacggcagc
    29461 atcattttta tcaagacgaa catacggttt ggtcatcctc ttctcctttc gagagtcagt
    29521 ggtacgtctg aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    29581 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    29641 aataaaaaca atcagatgag attaatcatc tcttcagcat tagcgttatg aatgagtaat
    29701 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    29761 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    29821 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg
    29881 tgttaaatac actcgtgccg ctttggctcg cccaggaaca catgtccaca tggcaggtag
    29941 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    30001 tcattaaacg cattggcttt aaccgcagct attatctgtc ctccttcatt tttgctgctg
    30061 gctgtgccgg ccttggcctg atgattggtt tctggagctg gttggcgtgg cgttttgtcg
    30121 cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag cgcgctgatg tgcagtggga
    30181 cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat ggtttattac gtgggaacgt
    30241 ttttaggcca gttactggtc agcaaagtct caaccgagct gatgagcgta ttgccgtggg
    30301 ttacaggttt gacattggca gggatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    30361 aggctgaaaa ccatgattcg acgtcaatta cggcaatgct aaaactccgt caggcgcggc
    30421 ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg atctctatat ggcctgatgc
    30481 ctctgtacct caatcacaaa ggggtgagca atgccagtat tggtttctgg atggcggtac
    30541 tggtcagtgc gggtatcctc ggacaatggc cgattggacg tctggcggat aagtttggtc
    30601 gactgctggt tttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    30661 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    30721 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    30781 aagccttact gttgagctat accgtgggaa gtctgcttgg cccgtcattt agcgctatgc
    30841 taatgcagaa tttctccgat aatttattgt ttatcatgat cgccagcgta tcgtttatct
    30901 atttgctgat gctgcttcgc aacgccggtc atacgccgaa acccgttgct cacgtgtaaa
    30961 tgaattcaag caaagtgtga acttactgtt tcacactctg cttttttgtt tcttctatct
    31021 gacttgcttt tttccaaatt ttattcgttt aaaaataaaa tgtgcagcag gttataattt
    31081 tgcatttcgc tatttccgca cttcttattt gccgcgcata atccctcgtt ttacagatgc
    31141 ccctttaatt ttggcgaagg atttgtctat ggctgggaat gttcag
//
LOCUS       DADLCQ010000043        30231 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000043 DADLCQ010000000
VERSION     DADLCQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.043"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..735
                     /locus_tag="JQG95_001754"
     CDS             <1..735
                     /locus_tag="JQG95_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY5888330.1"
                     /translation="SLSPSQLRRFTHNDVAHLARLLASPCPGQQLVVTEGVFSMDGDS
                     APLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRGSCWLQKVKPELLVVTFGKGFGVSG
                     AAVLCSSTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRRDEGDARREKLVSLIA
                     RFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQLAEKLRQQGCWVTAIRPPTVPAGTA
                     RLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            722..1477
                     /gene="bioC"
                     /locus_tag="JQG95_001755"
     CDS             722..1477
                     /gene="bioC"
                     /locus_tag="JQG95_001755"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY5888331.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            1470..2147
                     /gene="bioD"
                     /locus_tag="JQG95_001756"
     CDS             1470..2147
                     /gene="bioD"
                     /locus_tag="JQG95_001756"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY5888332.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            2726..4747
                     /gene="uvrB"
                     /locus_tag="JQG95_001757"
     CDS             2726..4747
                     /gene="uvrB"
                     /locus_tag="JQG95_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY5888333.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLAEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(4939..5847)
                     /gene="yvcK"
                     /locus_tag="JQG95_001758"
     CDS             complement(4939..5847)
                     /gene="yvcK"
                     /locus_tag="JQG95_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY5888334.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      6112..6256
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6244..7233
                     /gene="moaA"
                     /locus_tag="JQG95_001759"
     CDS             6244..7233
                     /gene="moaA"
                     /locus_tag="JQG95_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY5888335.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            7255..7767
                     /gene="moaB"
                     /locus_tag="JQG95_001760"
     CDS             7255..7767
                     /gene="moaB"
                     /locus_tag="JQG95_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY5888336.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            7770..8255
                     /gene="moaC"
                     /locus_tag="JQG95_001761"
     CDS             7770..8255
                     /gene="moaC"
                     /locus_tag="JQG95_001761"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY5888337.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            8248..8493
                     /gene="moaD"
                     /locus_tag="JQG95_001762"
     CDS             8248..8493
                     /gene="moaD"
                     /locus_tag="JQG95_001762"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY5888338.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            8495..8947
                     /gene="moaE"
                     /locus_tag="JQG95_001763"
     CDS             8495..8947
                     /gene="moaE"
                     /locus_tag="JQG95_001763"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY5888339.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            9084..9788
                     /locus_tag="JQG95_001764"
     CDS             9084..9788
                     /locus_tag="JQG95_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY5888340.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            9993..10706
                     /locus_tag="JQG95_001765"
     CDS             9993..10706
                     /locus_tag="JQG95_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY5888341.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLEQAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(10742..11698)
                     /locus_tag="JQG95_001766"
     CDS             complement(10742..11698)
                     /locus_tag="JQG95_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY5888342.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11698..12939)
                     /gene="clsB"
                     /locus_tag="JQG95_001767"
     CDS             complement(11698..12939)
                     /gene="clsB"
                     /locus_tag="JQG95_001767"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY5888343.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGIQVFEYRRRPLHGKVALMVDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(12936..13697)
                     /locus_tag="JQG95_001768"
     CDS             complement(12936..13697)
                     /locus_tag="JQG95_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY5888344.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13830..14240
                     /locus_tag="JQG95_001769"
     CDS             13830..14240
                     /locus_tag="JQG95_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888345.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(14202..15308)
                     /locus_tag="JQG95_001770"
     CDS             complement(14202..15308)
                     /locus_tag="JQG95_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5888346.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(15319..16452)
                     /locus_tag="JQG95_001771"
     CDS             complement(15319..16452)
                     /locus_tag="JQG95_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5888347.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(16445..18181)
                     /locus_tag="JQG95_001772"
     CDS             complement(16445..18181)
                     /locus_tag="JQG95_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5888348.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(18174..19172)
                     /gene="hlyD"
                     /locus_tag="JQG95_001773"
     CDS             complement(18174..19172)
                     /gene="hlyD"
                     /locus_tag="JQG95_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY5888349.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYNLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(19172..19843)
                     /gene="cecR"
                     /locus_tag="JQG95_001774"
     CDS             complement(19172..19843)
                     /gene="cecR"
                     /locus_tag="JQG95_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY5888350.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            20072..21259
                     /gene="rhlE"
                     /locus_tag="JQG95_001775"
                     /pseudo
     CDS             20072..21259
                     /gene="rhlE"
                     /locus_tag="JQG95_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(21466..21948)
                     /gene="ybiA"
                     /locus_tag="JQG95_001776"
     CDS             complement(21466..21948)
                     /gene="ybiA"
                     /locus_tag="JQG95_001776"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY5888351.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            22068..24218
                     /gene="dinG"
                     /locus_tag="JQG95_001777"
     CDS             22068..24218
                     /gene="dinG"
                     /locus_tag="JQG95_001777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY5888352.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            24246..25208
                     /gene="ybiB"
                     /locus_tag="JQG95_001778"
     CDS             24246..25208
                     /gene="ybiB"
                     /locus_tag="JQG95_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY5888353.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            25349..26434
                     /locus_tag="JQG95_001779"
     CDS             25349..26434
                     /locus_tag="JQG95_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY5888354.1"
                     /translation="MKSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(26663..26923)
                     /locus_tag="JQG95_001780"
     CDS             complement(26663..26923)
                     /locus_tag="JQG95_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5888355.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(27188..27454)
                     /gene="ybiI"
                     /locus_tag="JQG95_001781"
     CDS             complement(27188..27454)
                     /gene="ybiI"
                     /locus_tag="JQG95_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY5888356.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(27528..28241)
                     /gene="ybiX"
                     /locus_tag="JQG95_001782"
     CDS             complement(27528..28241)
                     /gene="ybiX"
                     /locus_tag="JQG95_001782"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY5888357.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(28247..30231)
                     /locus_tag="JQG95_001783"
                     /pseudo
     CDS             complement(28247..30231)
                     /locus_tag="JQG95_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         7103 a         7811 c         8162 g         7155 t
ORIGIN      
        1 agtttaagcc cgtcgcagct tcgccgtttt actcataacg atgtcgctca tctggcgcga
       61 ctgcttgctt ccccctgtcc ggggcagcaa ctggtggtga cagaaggcgt gttcagcatg
      121 gacggcgata gcgcgccact ggcggaaatc cagcaggtaa cgcaacagca caatggctgg
      181 ttgatggtcg atgatgccca cggcacgggc gttatcgggg agcaggggcg cggcagctgc
      241 tggctgcaaa aggtaaaacc agaattgctg gtagtgactt ttggcaaagg atttggcgtc
      301 agtggggctg cggtgctttg ctcaagtacg gtggcggatt atctgctgca attcgcccgc
      361 caccttatct acagcaccag tatgccgccc gctcaggcgc aggcattacg tgcgtcgctg
      421 gcggtcattc gccgtgatga gggggatgca cggcgcgaaa aactggtgtc actcattgcg
      481 cgttttcgtg ccggagtaca ggatttgccg tttacgctgg ctgattcatg cagcgccatc
      541 cagccattga tcgtcggtga taacagccgt gcgttacaac tggcagaaaa actgcgccag
      601 caaggctgct gggtcacggc gattcgcccg ccaaccgtac ccgctggtac tgcgcgactg
      661 cgcttaacgc taaccgctgc gcatgaaatg caggatatcg accgtctgct ggaggtgctg
      721 catggcaacg gttaataaac aagccattgc agcggcattt ggtcgggcag ccgcacacta
      781 tgagcaacat gcagatctac agcgccagag tgctgacgcc ttactggcaa tgcttccaca
      841 gcgtaaatac acccacgtac tggacgcggg ttgtggacct ggctggatga gccgccactg
      901 gcgggaacgt cacgcgcagg tgacggcgtt agatctctcg ccgccaatgc ttgcgcaagc
      961 ccgccataaa gatgccgcag atcattatct ggcaggagat atcgaatccc tgccgttagc
     1021 gactgcgacg ttcgatcttg catggagcaa tctcgcggtg cagtggtgcg gtaatttatc
     1081 cacggcactc cgcgagctgt atcgggtggt gcgccccgga ggcgtggtcg cgtttaccac
     1141 gctggtgcag ggatcgttac ccgaactgca tcaggcgcgg caggcggtgg acgagcgtcc
     1201 gcatgctaat cgctttttac cgccagatga aatcgaagag tcgctgaacg gcgtgcgtta
     1261 tcaacatcat attcagctca tcacactatg gtttgatgat gcgctcagtg ccatgcgttc
     1321 gctgaaaggc atcggtgcta cgcatcttca tgaagggcgc gacccgcgaa tattaacgcg
     1381 ttcgcagttg cagcgattgc aactggcctg gccgcaacaa caggggcgat atcctctgac
     1441 gtatcatctt tttttgggag tgattgctcg tgagtaaacg ttattttgtc accggaacgg
     1501 ataccgaagt ggggaaaacc gtcgccagtt gtgcactttt acaagccgcg aaggcagtag
     1561 gttatcggac ggcaggttat aaaccggtcg cctctggcag cgaaaagacc ccggaaggct
     1621 tacgtaatag tgacgcgctg gcgttacagc acaacagcag cctgcagctg gattacgcaa
     1681 cggtaaatcc ttacaccttc gcagaaccca cctcgccgca catcatcagc gcccaagagg
     1741 gcaggccgat agaatcattg gtaatgagcg ccggattacg cgcgcttgaa caacaggctg
     1801 actgggtgtt agtggaaggt gctggcggct ggtttacgcc gctttctgac actttcactt
     1861 ttgcagattg ggtaacacag gaacaactgc cggtgatact ggtagttggt gtgaaactcg
     1921 gctgtattaa tcacgcgatg ttgactgcgc aggcaataca acacgccgga ctgactctgg
     1981 cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg tcacgctgaa tatatgacca
     2041 cgctcacccg catgattccc gcgccgctgc tgggagagat cccctggctt gcagaaaatc
     2101 cagaaaatgc ggcaaccgga aagtacataa accttgcctt gttgtagcca ttctgtattt
     2161 ggttaaattg cgagcgagat cgcgtcttcg attgactgca atttaaccaa ttaaattcta
     2221 aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc gaatgtggga attgcccagg
     2281 cggcggggga taggggctgg agacagttat ccactattcc tgtggataac catgtgtatt
     2341 agagttagaa aacacgaggc aagcgagaga atacgcggct tgcacgcgaa ttggcgttaa
     2401 agacggctca aagaaatatc ttttattttt taactggtta gataaatgca atggcagtca
     2461 ctgaacaggc atctcttgcc ataaaactgt catcactcat cttgacaaat gttaaaaaag
     2521 ccgttgcttt ggggataacc cggtaaggct ggagttttat ctcgccacag agtaaatttt
     2581 gctcatgatt gacagcggag tttacgctgt atcagaaata ttatggtgat gaactgtttt
     2641 tttatccagt ataatttgtt ggcataatta agtacgacga gtaaaattac atacctgccc
     2701 gcccaactcc ttcaggtagc gactcatgag taaaccgttc aaactgaatt ccgcttttaa
     2761 accttctggc gatcagccag aggcgattcg acgtctcgaa gaggggctgg aagatggcct
     2821 agcgcaccag acgttacttg gcgtgactgg ctccgggaaa accttcacca ttgccaatgt
     2881 cattgctgac cttcagcgcc caaccatggt acttgcgccc aacaaaacgc tggcggccca
     2941 gctgtatggc gaaatgaaag agttcttccc ggaaaacgcg gtggagtatt tcgtctccta
     3001 ctacgactac tatcagccgg aagcctatgt accgagttcc gacaccttca ttgagaaaga
     3061 tgcctcggtt aacgaacata ttgagcagat gcgtttgtcc gccaccaaag cgatgctgga
     3121 gcggcgtgat gtgattgtag tggcgtctgt ttccgcgatt tatggtctgg gcgatcctga
     3181 tttatatctc aagatgatgc tccatctcac ggtcggtatg attatcgatc agcgcgcgat
     3241 tctgcgccga ctggcggagc tgcaatacgc tcgtaatgat caagcattcc agcgtggtac
     3301 tttccgcgtt cgtggcgagg tgatagatat cttcccggca gaatcggatg acattgcact
     3361 tcgcgtggaa ctgtttgacg aggaagtgga acgattgtcg ttatttgacc cgctgaccgg
     3421 gcagattgtt tccactattc cacgttttac catctacccg aaaacgcact acgtcacacc
     3481 gcgcgagcgc atcgtacagg cgatggagga gatcaaagaa gaactggccg ccagacgcaa
     3541 agtgctgttg gaaaataaca aactgctgga agagcagcgg ctgacccagc gtacccagtt
     3601 tgatctggag atgatggacg agctgggcta ctgttcgggg attgaaaact actcgcgctt
     3661 cctctccggt cgtggaccgg gtgagccacc gccgacgctg tttgattacc tgcctgccga
     3721 tgggctgctg gtcgtcgatg aatctcacgt caccattcca caaattggcg gcatgtatcg
     3781 cggtgaccgg gcgcgtaaag agacactggt ggagtacggc ttccgcctgc catcagcgct
     3841 ggataaccgt ccgcttaagt ttgaagagtt cgaagcatta gcgccgcaaa ccatctatgt
     3901 ttcggcgacg ccgggtaatt acgagctgga aaaatccggc ggcgatgtgg tggatcaggt
     3961 ggtgcgtcca accggattgc ttgacccgat tatcgaagtg cggccggtgg cgacacaggt
     4021 tgatgatctt ctttcggaga ttcgtcagcg agcggcaatt aacgaacgcg tgctggtcac
     4081 cacactgacc aagcgaatgg cggaagatct tgccgaatat ctcgaagaac acggtgagcg
     4141 cgtgcgttat cttcactcag atatcgacac cgtcgagcgt atggagatta tccgcgactt
     4201 gcgtctgggt gagttcgacg tattggtagg gatcaactta ctgcgtgaag gtctggatat
     4261 gccggaagtg tcgctggtgg cgatcctcga cgctgacaaa gaaggcttcc tgcgttccga
     4321 acgttcgttg atccagacca ttggtcgtgc ggcacgtaac gttaacggta aagcgattct
     4381 ctacggcgat aggagcactc catcaatggc gaaagcgatt ggcgaaaccg aacgtcgccg
     4441 tgagaaacag cagaagtaca acgaggaaca cggcattacg ccgcaaggct tgaacaagaa
     4501 agtggtcgat atcctggcgc tggggcagaa cattgccaaa accaaagcga agggcagagg
     4561 aaaatcgcgc ccgattgttg agccggataa tgtgccgatg gatatgttgc cgaaagcgtt
     4621 gcagcagaaa atccatgaac tggaagggct gatgatgcaa cacgcgcaga atctggagtt
     4681 cgaagaagcg gcacaaattc gtgaccagtt gcatcagttg cgtgagttgt ttattgccgc
     4741 atcgtaacag gatagcgaag aagactgatg acaaacggaa aacagtctga tgcgctacgc
     4801 ttatcaggcc tacattttct ccgcaatata ttgaatttgc gcggtttgta ggccggataa
     4861 ggcgttcacg ccgcatccgg cataaacaac gcgcactttg tcttcaatag gaagccggaa
     4921 ttttccttcg gatttccgtt aacctaaagc ctgtaacgcc ttttccagcg cgttatgtaa
     4981 caactggcgg tcatgacgat acggaatatc gctggcctcc agtacctcct ggatcacaat
     5041 ccgctctttc accgccgaga catccacttt tggcccgacg atgaccgcat caatgacctt
     5101 tttaccaaca tactgctcca taattgccag cttgctttcc agcttcaaat tagccgcagg
     5161 taaactcaac tcacgcccca gattgccgat ataaaccatc ggcgctggcg tgcggcgtaa
     5221 tgcctgggcg atttccttca gcagcagaat tggcatcagg ctggtataaa aactgccagg
     5281 cccaataatg atgagatccg cttcattgat agcgtgaacc gcctcacgcg ttgcgggtac
     5341 attaggcgtt aacaataact cttgaatcgg cgtagttaac tggtcgatat tgacctcgcc
     5401 gtaaacttca tgcccctgat cgtcaatcgc catcagatca acaggatgct ctgacattgg
     5461 aatcaaatgc gtatccactt tcagcagatt acgaattaaa ttgatggctt ccagaggccg
     5521 cacgctaagg tgatccagcg cctttaacat caagtttccg agattatgac cggaaagttc
     5581 gccattgccg ccaaaacggt attcaaacat cgcggaggcg acgttcggtt ccgttatcag
     5641 ctggttgagg cagttgcgca tatcgcccca agcaatgccg ccttctgaac ggcgaatacg
     5701 ccccgtcgag ccaccattat ccgtggtggt gacgataccc gttaaacgag aacccaaaga
     5761 cgaaagtgat gagagaacgc gtcccagtcc atgccctccg ccgagagcaa cgacacgatc
     5821 aagatcagcc agcgtacgat tgcgcatata gtttcctgga gtcagattat ccgcgctaca
     5881 gtagcgcaaa ttgcggggaa acagcaatta acctgccaat attgaggata caaactattt
     5941 tcttctatct ctaaaggacg atgcacgcta tgcctccctg atgatgtata tcaaagtaaa
     6001 accgccattt tcccttattc tgtagcgaaa tagcacgatc atgacgctat atacatgatt
     6061 acatagcgaa agtgtggatg gtaaaaatct catttacacg ctagtatcgg cttaaccact
     6121 aaacactcta gcctctgcac ctgggtcaac tgatacggtg ctttggccgt gacaatgctc
     6181 gtaaagattg ccaccagggc gaaggaagaa atgacttcgc ctcccgtatc tggaaaggtg
     6241 tacatggctt cacaactgac tgatgcattt gcgcgtaagt tttactactt gcgcctgtcg
     6301 attaccgatg tgtgtaactt tcgttgcacc tactgcctgc cggatggcta caaaccgagc
     6361 ggcgtcacca ataaaggctt tcttaccgtc gatgaaattc gccgggttac gcgcgccttc
     6421 gccagtctgg gcaccgaaaa agtgcgcctg acaggaggag agccgtcttt acgccgcgac
     6481 tttaccgata tcatcgccgc tgtgcgggaa aacgacgcta tccgccagat tgcggtcacc
     6541 accaatggtt accgtctgga acgcgatgtg gcgaactggc gcgatgcggg acttactggc
     6601 attaacgtca gtgtcgacag tttggacgcc cgccagtttc acgctattac cgggcaggat
     6661 aaattcaacc aggtcatggc agggattgat gctgcatttg aggccggttt tgagaaggtc
     6721 aaagtcaata ccgtgctgat gcgtgatgtt aatcatcacc agctcgacac ctttctgaac
     6781 tggatccagc atcgccctat ccagctgcgt ttcatcgaac tgatggaaac gggcgagggc
     6841 agcgagctct tccgtaagca tcacatctct ggtcaggttc tgcgtgacga gctactgcgt
     6901 cgcggctgga tccaccaatt acgtcaacgc agcgacggtc ccgcgcaagt cttttgccat
     6961 ccagattacg ccggagagat tggccttatc atgccgtatg aaaaagactt ctgcgccact
     7021 tgcaaccgcc tgcgcgtttc ctccattggt aaactccatc tctgcctgtt tggtgaaggc
     7081 ggcgttaacc tgcgcgatct gctggaaggc gatacccagc aacaggcgct ggaagcgcgt
     7141 atttcagcgg cgctgcggga gaagaaacag acccatttcc tgcatcaaaa caacaccggt
     7201 attacgcaaa acttatcgta cattggcggc taaaacgtca aaaggagaga tcagatgagt
     7261 caggtaagca ctgaatttat cccgacccgt attgctattc ttacggtttc taatcgtcgc
     7321 ggtgaagaag acgatacctc cggtcactat ctgcgcgatt cggcgcaaga agcgggccat
     7381 cacgttgtcg atgaagccat tgtgaaagaa aaccgctacg ctattcgcgc tcaggtatct
     7441 gcgtggatcg ccagcgacga tgtacaagtg gtattgatta cgggtggtac tggcctgacg
     7501 gaaggtgatc aggctcccga agcgctgctg ccgttgttcg accgtgaggt tgaaggtttt
     7561 ggtgaagtgt tccgtatgtt gtcgtttgaa gagattggca cttccacgtt gcaatctcgt
     7621 gcggtagcgg gcgtcgctaa caaaacgctg attttcgcca tgccgggttc gaccaaagcg
     7681 tgccgtaccg catgggaaaa tatcatcgcg ccgcagctgg atgcccgtac gcgtccgtgt
     7741 aatttccatc cacatttgaa gaaataagta tgtcgcaact gacccatatc aacgccgctg
     7801 gcgaagcgca catggtggat gtctccgcca aagcggaaac cgtgcgtgaa gcgcgggcgg
     7861 aagcctttgt caccatgcgc agcgagacgc tggcgatgat tattgatggt cgccaccaca
     7921 aaggcgacgt atttgccact gcgcgtattg ccggtattca ggcggcaaaa cgcacttggg
     7981 atctgatccc gctgtgtcac ccgctgatgc tcagcaaagt tgaagtcaat ttacaggccg
     8041 agccggagca caatcgggtg cgtatagaaa ccttatgccg cctgaccggg aaaaccggtg
     8101 tcgaaatgga agcgttaacc gcggcctccg tggcggcgct gaccatttat gacatgtgca
     8161 aagcggtgca aaaagatatg gtgattggtc cggtacgttt gctggcgaag agcggcggca
     8221 agtcgggtga ctttaaggtg gaagcggatg attaaagttc tttttttcgc ccaggtgcgc
     8281 gagttggtgg gaacagatgc aaccgaagtg gctgcggatt acccaaccgt tgaagcgtta
     8341 cgccagcacc tggctgcgca gagcgatcgc tgggcgctgg cgctggaaga tggcaaattg
     8401 ctggctgccg tcaaccagac gctggtgagt tttgaccatc cgctgactga cggcgacgaa
     8461 gtagctttct tcccgccggt aaccggaggt taagatggca gaaaccaaaa ttgttgtcgg
     8521 cccgcagccg ttcagcgtag gagaagagta cccgtggctg gcggagcgtg acgaagacgg
     8581 tgcggtagtc accttcaccg gaaaggtgcg caaccataac ctgggcgaca gcgtcaaagc
     8641 attaaccctc gaacactatc cggggatgac tgaaaaagca ctggcagaaa ttgttgatga
     8701 agcgcgtaac cgctggccgc tggggcgcgt cacggtgatt caccgcatcg gggaattatg
     8761 gccgggcgat gaaatcgttt ttgtcggtgt caccagtgcg catcgcagca gtgcgtttga
     8821 agccgggcag tttattatgg attatctcaa aacccgcgca ccgttctgga agcgcgaagc
     8881 cacgccggaa ggcgaccgct gggttgaagc ccgggagagc gatctgcagg cggcaaaacg
     8941 ctggtagttt tttgttagcc ggataaggca ccgctgcgtc cggcaatttc accgctcagt
     9001 acatgcgttt acgctgcgtc gatgcacagc ctcatcattt tgcagtatcc ttaagatatt
     9061 ccttatatct tcaggagatc gtcatggaca gattcccacg ttctgattca atcgtacaac
     9121 cccgggctgg cttgcaaact tatatggcgc aagtctatgg ctggatgacc gttggcttgt
     9181 tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc ggccgtgatg gagctgttgt
     9241 tcactaaccg tgtcttttta atcggtctga tcatcgcgca attagcattg gttattgtgt
     9301 tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac gatgctcttt atgctttatt
     9361 cggcgctgac gggtcttacg ctttccagta tattcattgt ctataccgct gcttctatcg
     9421 ccagtacttt cgtcgttact gccgggatgt tcggcgcaat gagcctgtac ggttacacca
     9481 cgaagcgcga tttaagtggc tttggcaata tgctgtttat ggcgttaatc ggcattgtgc
     9541 tggcatcgct ggtcaacttc tggctgaaaa gcgaagcatt gatgtgggca gttacctaca
     9601 tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac tcagaaattg aaaaatatgg
     9661 gtgagcagat tgatacccgc gacacgtcga acctgcgcaa atattccatt cttggtgcgt
     9721 taaccttgta tctggacttc atcaacctgt tcctgatgtt gttacggatc ttcggcaacc
     9781 gccgttaatt ctttttcacc atcactatcc cggccatgtg tcgggatttt tttcttacta
     9841 aaaacgacac ttaacaagta ccaggtataa aatatgcctt atgagcgagc ggaatcgttt
     9901 tccgtatcca taaatacaaa agtattttgt atgcgtggtt ttaaatttat tgatggtgaa
     9961 tattaatgtt ttttctaagg acaactaaaa gaatggaatc atactcgcaa aacagcaata
    10021 aattagattt tcagcacgag gccaggatat taaacggtat atggctcatt accgctttag
    10081 gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt acggcaacca
    10141 aatatgattc accaccaatg tatgtcgcca tagggttatt attactttgt atgtatggct
    10201 taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat ctgtttttac
    10261 tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt atcatcgtgt
    10321 tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta tttaatgttg
    10381 atcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc ctggtaatca
    10441 tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc ttaatgattg
    10501 tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaacag gcaggaaaat
    10561 gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca ttaacactct
    10621 attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg cttgtctggc
    10681 aaaggcatac gcgttttttt cattagtcgt aaatgcagat actaaagaac ttaactaaac
    10741 ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc ttggctttcc agtagcaggt
    10801 aacagatcag cgccagcagc agcgggataa agtaatacag gacgcgataa gcgagcaggg
    10861 cggcgataat gctccctttc gaggtatgct ccccagccag tagtgcgata aacaccgctt
    10921 ccagcacacc gatccccgcc ggaatatgca cgatgacgcc agcaatacta ctaaccagta
    10981 acacgcccag tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc
    11041 ccattaccat ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag
    11101 gcagcaccag cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc
    11161 acaaataaac cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat
    11221 cgacatacca gtaatccggc aactccacca cgcctgcggt aaagataatc cctgccagta
    11281 aaatatagcc cagccagttg gtggtaatac tgagcgagga aatccgcgta atagtgctgc
    11341 ccggtaaccc tagtcgggag tacaaacgat agcgcatacc aatgccgccg acccaggtac
    11401 tgagcgtcag gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca
    11461 gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga
    11521 cgaccaccag cccgaccgca ctaagcagcg caacgcgatt gtagtcgcgg atgaccttcc
    11581 agacctcttc ccagtccact tttttggcgt agaccaccag caacacaatc accgcgataa
    11641 aaaacagcca ggtgaggatc ttctttgcta agcgccagcg cgggtgtgat ttactcatca
    11701 ggggtttacc cccgtgtttt cagtttctac ccgatcctgc gtttccattg tcggttgtgc
    11761 ggggggatca acctgcgtca gacgtggtgt gtgtgccgga agccagccaa ccagcgccgg
    11821 gaagtggcgt aaaaagtgga acgccagcac gcttttggtc aggttccacc aggtgcgttt
    11881 gggcagcatg gtttcatcca cctgctgaca atctgcggca ataatgccgt tcagattatc
    11941 gcgcagcgtc tggttaaaat gacgatcgtg gatgatgaca tttgcttcga gattcagtga
    12001 caaactgagc ggatcgagat tactggaccc tactgtcgcc cagtgatcga ccatcaatgc
    12061 cactttgccg tggagcgggc ggcggcggta ctcaaaaacc tgaatgccgc ctttaaccag
    12121 atagttatac agcaagcgcg caccgactct gacaatcggc atatccggtt cgccctgaat
    12181 gatcagtttg atccgcaccc cgcgccgtgc cgctttacgc aaggcgtgta aaaatcgata
    12241 gccggggaag aagtaggcgt tggcgataat cacttcccgc tgcgcctgag tgagcatttt
    12301 cagataatgg cgttcaatat catcgcgatg ttcttcgtta tcgcgccaga ccagcaatac
    12361 ctgcgcttct cccggctggc ggttctcttc cgctttgtga tgacgtcgcc accagcgtcg
    12421 tgccgcgctc tgtccaggca ggttttccag ctcaaactgg agaatatctt cgacaatcgg
    12481 cccttcaagg cgtaccgcgt aatcctgttt agcctctgga ccgtagctgg acatatgctc
    12541 ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg tcgatcacca caattttgcg
    12601 atgcatccgg cgaaacacat tggtgcgcat accaaaaagg cgagggcggg gatcgtagta
    12661 gcggaacact acgccagctg ccgtcagttc attgacaaac tcatcgctga gatccggcga
    12721 accgtagcca tccagcaaga cttccgcttt aaccccgcgt tgcgctgctg ccagtagtgc
    12781 cgcatgcagc tgtttgccga cgttatcctc aaaccagata aacgtttcaa gaatgatgcg
    12841 ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa tattgctcgc cgttttccag
    12901 caactggatc ttattgcctt cgcgccagct acatttcata aatgaatctc cgcacttaaa
    12961 ggggcatgat cagaaaggtg tcgccatgtc cgcagcggca acgcggttgg cgcgctggcg
    13021 ctggcatttt tgacgtagat cctgtccagt cgtagtagag gaaattgcac cggaaacgtg
    13081 cgcgccgggc gtccgtgggc gcgggtaaaa atctcatcca gtccggcctg cacttttaac
    13141 ggatgattag ctttttgccg ccagtcattg aaatcacccg ccaccaatac cggttcgccg
    13201 tccggtagct cattcaccca ttcggcgagc atcgcaagct gcgcctgacg gtgcgcctca
    13261 cgcaggccca gatgtacgca catcacatga atcgcttttc cggtcatcgg cggcacaatg
    13321 cggcagtaga gcacgccgcg cttttccgca ccatcgaccg aaacatcgcg attctcataa
    13381 tgttcaatgg gataacgcga cagtacggcg ttgccgtgat gcccttccgg gtatacggca
    13441 ttgcgaccgt aggcaaaatc gctccacata gtgtcggcga gaaactcgta gtgcgaggta
    13501 tcgggccagt tttccacatg cagcggatga acttcgtgcg cgcccatcac ttcctgcagg
    13561 caaacaatat cggcgctgac ggtacgcacg gcatcgcgaa gttccggcaa aatgaagcgt
    13621 cggttaaacg cggtaaagcc tttgtgaatg ttgatggtga gcaccttgaa cgaaaattgt
    13681 tgtgtttgat cgggcataat tttcctgtct ttgcctctta tctcattgaa atagtgtagt
    13741 cggcgtcaca aaaaggtgcg gtcttacgga attttccgta aagttcggta ctctgagtaa
    13801 gtagagataa attcttcagg agagaagcca tgaagtggca acaacgtgtt cgtgtcgcaa
    13861 cgggtctaag ttgctggcag attatgttgc atttactggt agtggcgctg ctggtggtgg
    13921 gctggatgag taagactctg gttcacgtcg gcgtgggatt atgcgcactg tattgtgtca
    13981 cggtagtgat gatgctggtg tttcagcgcc accccgagca acgctggcgt gaggtggcag
    14041 acgtgctgga agagctgacc acgacctggt attttggcgc agcgctgatt gtgctgtggc
    14101 tgttgtcccg cgtactggaa aacaactttt tgctggcaat tgcagggctg gcgatccttg
    14161 ctggcccggc ggtagtgtct ttgctggcga aagataagaa gttacatcac cttacgtcta
    14221 aacatcgcgt acgccgctga ccctgtcgtg gccgttatca ccagtagcgg ccacaaacta
    14281 ttccacacaa tatccagact cgcatccttc aaataaatct gcttggtaat gtccgtaaag
    14341 tggcgaatag ggttaatcca cgtcagattt tgcagccaca ctggcatgtt ttccaccggc
    14401 gaaacgtaac cggaaaggag aatggcgggc atcataaaaa caaacacgcc gataaacgcc
    14461 tgctgttgtg tcgagcagag tgatgaaatc aacagaccga atcccaccag cgataaaccg
    14521 taaatcacca tcgtaaagta gaacagcgct agcgatccgg cgaaggggat ttgatacgcc
    14581 cagataccaa tcgccagcac aatggtggcc tggaaggtgg cgacaattaa cgccggtacg
    14641 gctttgccga tgaagatctg ccaggtggtg agcggcgaaa ccagtagctg atcgagcgta
    14701 ccttgttcac gttcgcgggc gacggaaagt gaagtgacga tcattacgcc gatagtggtg
    14761 atcatggcga tcagcgacgg caccacaaac catttgtagt cgagattcgg gttataccag
    14821 ttgcgtacca ccagctcgct gttgttgggt ttcggttttc cttccagcag ctcctgctga
    14881 taatttttga cgatctgttg caggtagttg gcggcaattt gcgcactgtt ggagttacgc
    14941 ccgtcgagga tcaactgcaa cggcgcggtc tggaaggtat ccagtttgcg cgagaagtca
    15001 gccgggaaac gcaccagtag taacgccttt tgtgtgtcga tggtcgggcg gatctcctgt
    15061 gggcttttca gcagcagcac atgtgtaaag gcgctggcgc gggcaaaacg ttgagtaagt
    15121 tccaccgaat gctcgccgtt atcttcatcg tagatggcga tggtggcgtt agtcacttcc
    15181 agcgtggcgg cgaacgggaa caggatcacc tgaattagca cgggtaaaat cagaatcgcg
    15241 cgggtttgtg gttcgcgcag caacgactgc aactctttgc ggattaacgt ccataagcga
    15301 tgaaacatgc tcttctccct aatccagccg acgtttggtt ttcagccacg tcaggccgat
    15361 aaacatcacc gccgaagcga tcaaaaacag cacgtttacc accagcacca ctggaatatt
    15421 cccggcgagg aacaggcttt gcagggtgct gacgaaataa cgagcgggaa taatgtacgt
    15481 caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa ataaagccgg aaagcataat
    15541 cgacggcaga aaagcggcgt tcagggcgac ctgagcggca ttgaactggt tgcgggtaat
    15601 cgtggaaatc agcagcccca ttcccagggt actgagtaaa aacaggctgg agataaaaaa
    15661 cagaatcagc agcgaaccgc gatacggcac gccgagaata aacactgaca ccagcataca
    15721 cagcaacatc gccagcatcc cgagaaagta ataagggatc agcttacaca gcagcagttc
    15781 cgtgcgggta atctccgtag agagcagagc ctccatggtg ccgcgttccc attctcgcgc
    15841 caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg ataatggtca ccgcacccgg
    15901 gataataaag tgctggctta tcgccgccgg gttaaaccag tagcgggttt gtacatcaat
    15961 aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc atttgccaga tctgccagat
    16021 cccttcgaca tacccctgca caaagttagc ggtattcggc tcactgccgt cggtgatcac
    16081 ctgaatcggt gcggtggcgt tggcgcgctc catctgttcg gcgaaatcca cgggaataac
    16141 caccagaccg cgaattttcc ccgactgcat tttggcgatc agttcctgac ggttatcgct
    16201 gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg gtgaaatcca gcgccgcttc
    16261 gctacgctgt tccagcaaaa tcccgacccg cagcttgctg gagtcgaggt taatgccgta
    16321 accaaaaata aacagcagta gcagtgggat cactaccgca atcagccagc tactcggatc
    16381 gcgaacgatc tgccgcgtct ctttgacgca cagcgcccgc acgcgatgcc aggaaaggat
    16441 cgggttactc attgctatgc tccttatccc agtcgtggat caactgaata aaggcttgct
    16501 ccatggtggg atcgggttgc tcatcgttag ctgactgtgc tttcaaatcg tccggcgtac
    16561 cgctggcgat taatttccca cggtacacca ggccgatgcg gtcgcaatat tccgcttcat
    16621 ccataaagtg ggtggtgacc atcaccgtga cgcctttctc taccatgctg ttgatgtgca
    16681 gccaaaattc acggcgggtg agggggtcaa cgccggaagt cggttcgtcg agaaagagaa
    16741 tatccggttc atgcatcagc gaacaggcca gcgccagccg ctgtttaaaa cctaatggca
    16801 gttcatcggt ggcgtgtgag gcaatacttt tcaggccaaa tgcctcgctc atacgggaga
    16861 ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc agagaaaaag cgtaaattct
    16921 gttcgaccgt caggttgccg tagagcgaaa atttttgcgc catatagccg agatgctggc
    16981 gcgctttacc ggaactctct ttcagatcca tccccagcac cagcgcctgg ccggaagtcg
    17041 gcaccagcaa accgcacatc atcttaaagg tggtcgattt acccgcgccg tttggcccca
    17101 gcaaaccaaa aatctcccca cgtttaacgg caaagttgac gtgatcggtg gcggcaaaat
    17161 caccaaattt cttggtcagt tctttcgctt cgatcaccgt ctcgccgggt gtgccttcta
    17221 ccgtatgcaa tattgcaccc agcggcgatt ctgaggttcc ggcaccgccc agcaaatcaa
    17281 taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt gatttccggc atcccgtcgg
    17341 catggcgaat atcgtctggt gtggcctctt ttttgaggat cagacgtacc gatttcccct
    17401 gaatcatgcc atcgctgacc tgcggcagtt tcaaggcgcg ttgcaacagt ttgcggttgc
    17461 cctcgtgtgg actggtcatc agaaagctgc gtccggccat ggtttgtgtc agggcttttg
    17521 gttctccctg atacagcaac tcgccttcgt tcatcagtaa cacgtcacgg cactgctcgg
    17581 cttcgtcgag atacgaggta ctccagagga ttaacatccc ttcgcccgcc agctcatgca
    17641 ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc gacgccgggt tcatcgagca
    17701 gcaacacttt cggttcgccc accagggtac aggccaggcc gagtttttgt ttcatcccac
    17761 cggagagctt gcccgccagg cgtccggtaa acggcccaag agacgtaaac tccagcaggc
    17821 gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg caaatccgcg tacagattga
    17881 ggttctccat caccgtgaga tcttcataca gaccaaattt ctgcggcata tagccgagca
    17941 cggcgtgcag cgcgccgtcg tttttgatcg gatcaaagcc aatcaccgtg gcactgccgc
    18001 tgtcgggttt cagtaatccc gccaacatcc gcatcagtgt ggttttacct gcaccgtccg
    18061 gccccaccaa ccccgtcaca taaccggcgt gaatggtaca atcgagcggc gcgacggcgg
    18121 gcttgtccat gcccggaaag cgtttttcca ggccgttcag cgtgataacg gcatcattca
    18181 tgtcctgcct cgtcaccgaa ttgtaccgtc actggcattc cctggcgtaa cgcatcatcg
    18241 gcgtcggtca ccacaatacg caggcgatag acgaggtcgg tacgcagatc cggtgtttcg
    18301 acggttttcg gggtaaattc agcagtcggc gaaacgaaac caatctgccc gtgatacggc
    18361 ttgtccgggc gaccatcggt ataaagcagc actttgcgcc ccggctgggc ctggtcaaga
    18421 ttccgttcat caacataagc gcgcacccac accggacgcg ttagtgaaac ggtaaacacc
    18481 gtgccacctt cattgagaac cgtgcctggc tctaccgcgc gcgttaacag cgtgccatca
    18541 gacggtgcta tcaacgttga gtcctgtaaa ttcagctccg cctgcgccag ttgcgcctgc
    18601 gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt cacggttacc ggaacggtac
    18661 tgacgcaatt tatcctgtgc tgatttcagc gttgcctgtg cctggtcgcg cgaggagcgg
    18721 gcattttcca ggtcatttgc cgaaatggtg cggcttttcc acagtccttg ctggcggtta
    18781 tagaagttct gcgcatagtc ataggcggct tgcgcctgtt tcaccgctgc ggcggcctga
    18841 gcgatttctt catcgcgata cccggcaagc atcagattat actgcgcctg tgccaccgag
    18901 acgcccgctt tcgcctgcat cagggcaatc tcatacggct tgtgatccag ttcgcccagc
    18961 acctggcccg ctttgatagc atcaccttcg tccaccgcca gcgattcaac gcgcccccca
    19021 acacggaaac taagattcac cgtacgaata tccacgttgc catacagcgt caggccgtta
    19081 tcctggcggc tttgatacca ccagtagcct ccggcaacca cggcggcaag taccactacc
    19141 gccagtccga tcacgacagg ttttttcatc actacaaact cctttgcgat aatccttgca
    19201 aaatcaggtc gatatgacag gtcaccgtct ggttgatcag ttcggtcttt tcttcatcga
    19261 acgcggtcca gccggtacgt aacagaattg tttctttgcc aagacggaac gccagaatct
    19321 caccaatcaa cgcatgggta tgaaggatca tgcgggtgtc attagcgtcg cagccggtcc
    19381 aggcggcaat caggcgtgtc aggtggctgt gtagcggact aatcacctgt tcgtgcacca
    19441 ggtggtaggc tgccgtggga gagagctgct cacgggagat aaacttgctg aggttgacgg
    19501 tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc gcgaaggatc agttcacgga
    19561 tggcagcccg atcaggctgt ggttgggcga acaagcgttc ggcttcctcg gcatgcggac
    19621 ggaactgctc gccaataaaa tcggcaatcc actgggcgca ggcgaggtac aaatcttctt
    19681 tcgaaccgaa gtagtaggtg atggcagcga tattctgccc ggcctgggcg gctatctcgc
    19741 gggtggtggc gttcattcca tattcaccaa actgcgccag tgcggcagca atcagctgtt
    19801 ttttcgcctg ttcacccttg attgtcatgg caggattatt catcgcacag tccattctta
    19861 atcaaatgat tgattaagat tatgactcca tatgggagtt gtccagtatg gctaagaatt
    19921 ttagctacgc cagtcacagg gataatttat gcgctgcgtc acaaaaactg ctacactccg
    19981 ctccctcatg acattgtggt ttttgtcatt ttccttttca gtatctccct gaaaactaca
    20041 ccggtaacgg tcggggcggt tcggagtagt tatgtctttc gattctttgg gtttaagccc
    20101 tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa cccaccccta ttcagcagca
    20161 ggcgatccct gcggtgctgg aaggccgcga cctgatggct agcgcccaga ccggcaccgg
    20221 caaaacagcg ggctttacgc tgccgctgtt gcaacacctg atcactcgcc agccgcacgc
    20281 caaagggcgt cgtccggtac gtgcgctcat tcttaccccg acccgtgaac tggcggcaca
    20341 gattggcgaa aacgtccgtg attacagcaa atacctgaac attcgttcgc tggtggtgtt
    20401 tggcggtgtc agcattaacc cacaaatgat gaagttgcgc ggcggcgttg atgtgctggt
    20461 ggcaacgccg ggacgtttgc tggacctgga acatcaaaat gcagtgaagc tggatcaggt
    20521 tgaaatcctc gtcctcgatg aagctgaccg catgctcgac atgggcttta tccacgatat
    20581 ccgtcgcgtg ttaacaaaac tgccagcgaa acgccagaac ctgctgttct ctgcgacctt
    20641 ctctgacgat attaaagccc tggcggaaaa actgttgcac aacccgctgg aaatcgaagt
    20701 ggcacgccgc aataccgcgt ctgatcaggt gacccagcac gttcactttg tcgataagaa
    20761 acgcaaacgc gaattgctgt cgcacatgat tggtaaaggg aactggcagc aggtgctggt
    20821 gtttacccgt accaaacacg gcgctaacca tctggctgag cagctcaata aagatggcat
    20881 ccgtagtgtg gcgatccacg ggaataaatc gcaaggtgcg cgtacccgtg cgctggctga
    20941 ttttaaatcg ggtgacattc gcgtactggt ggcgaccgat atcgctgcgc gcggtctgga
    21001 tatcgaagag ttgccgcacg tcgtcaacca tgaactgcca aacgtaccgg aagattatgt
    21061 ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt gaagcgttgt cgctggtgtg
    21121 tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg ctgaaaaaag agatcccgcg
    21181 cattgcgatt ccgggctatg agccggaccc gtcaatcaaa gccgaaccga tccagaacgg
    21241 tcgccagcaa cgtggcggca tcaggctgat gaacaaacgc aaaactgcct gatgcgctac
    21301 gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt gtaggccgga
    21361 taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa tttgagaaca
    21421 cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact tctctatagc
    21481 caattgttcg cgcaactcca ttaaaaggta gcccagtcga ttcttgccct taccattacc
    21541 accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg gcgcggttgc
    21601 cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcgcgaagtg ctttacgcat
    21661 cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat tacgccccat
    21721 gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt tttcatcaag
    21781 gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gtattcccgt caaccttgat
    21841 gggcctggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat
    21901 gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta aaccgcatta
    21961 tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc ctgaaaaccg
    22021 aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg gcattaaccg
    22081 ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag atccccgact
    22141 ttattccccg tgcgccgcag cggcagatga ttgcggacgt cgccaaaacg ctggccggag
    22201 aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg ctctcctatt
    22261 tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca
    22321 acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa aagatcattc
    22381 ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtcca cgtaatctga
    22441 cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt gacgacgaac
    22501 tgacgccaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca
    22561 cttataaatg ggatggtctg cgcgatcata cggatatcgc tatagatgat gatctctggc
    22621 gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac tatcgtgaat
    22681 gcccgttttt tgttactcgt cgggagattc aggaagcgga agtggtggtg gcaaaccatg
    22741 cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa aatctgctgc
    22801 tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg gagatgagcg
    22861 ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa ctggtcgcta
    22921 cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct gaacgtttga
    22981 atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt ctcaatctct
    23041 acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg cctgatgaat
    23101 tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt
    23161 tatttcttaa cgatttaagt gagaaaaccg gcagccacga tattgtgcgt ctgcatcggt
    23221 tgattttaca gatgaaccgc gcgctgggga tgtttgaggt gcaaagcaaa ctctggcggc
    23281 tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc
    23341 gcgaagggca gctacacctc tggtttcact gcgtgggaat acgtgtcagc gatcagctgg
    23401 aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc
    23461 tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg ggcgaccgtt
    23521 ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt attccccgga
    23581 tgcgctttga gccttccatc gacaacgaag agcaacacat tgccgaaatg gcggcctttt
    23641 tccgtgaaca ggtggagagc aaaaaatatc tcggtatgtt ggtactgttt gccagcggac
    23701 gggcgatgca gcgctttctc gactatgtga cggatttacg cctgatgttg ctggtgcagg
    23761 gcgatcagcc gcgttatcgc ttagttgaac tgcaccgcaa acgcgttgcc aacggtgagc
    23821 gtagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc
    23881 tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccg gtggtgatca
    23941 ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa agcctgccga
    24001 gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac ggttgctggg
    24061 gcgaagtggt tatctacgac aaacgcttgc tgacaaaaaa ttatggcaag cgactactgg
    24121 atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata gttaaaaaga
    24181 aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt
    24241 cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg
    24301 atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt gaagtccctg
    24361 accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggggaaggg gaagcagaga
    24421 tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg cctgcaggca
    24481 agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggct aacctgacgc
    24541 cattattggc gattctgctg cataaactcg gttttcctgt ggtggttcac ggagttagcg
    24601 aagatccaac tcgcgtgctg acagaaacca ttttcgaact gatgggtatt acaccaacgt
    24661 tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt atgcctgtcg
    24721 gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta
    24781 acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgag gcactgtgtc
    24841 tttccagcgt ttcgcatccg gaatacattg gacgcgtcgc gaagttcttt agcgatatcg
    24901 gcgggtgggc gttactaatg catggtactg aaggcgaagt gtatgctaat ccgcagcgct
    24961 gtccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa aaacaggacc
    25021 ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg cagtggactg
    25081 agcgttgcct ggctggcagc gaagcgattc ccgaatcgct gaaaatccag atggcttgct
    25141 gcctggtggc tacgggtgaa gcggcaacta tcagcgatgg cctggcgcgc gttaatcagg
    25201 cattttaatt tttatctcag ccggatgagc cacgtcatcc ggcttttacc ccaccgcaat
    25261 atgaaattcc tgcatcttta ttgaccttcc cgcgcccggc gtgcagcata aaaaaacaac
    25321 aaacacataa cataaacagg agttaaccat gaaaagtggt catcgctttg atgctcagac
    25381 gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa
    25441 attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattctc atggtattgg
    25501 catgatccca agctatgtgc gttcctggag tcaggggcat ctgcaaatta accatcatgc
    25561 caaaatcgtt aaagaggcgg gggcggcagt cacgctcgat ggcgatcgcg cattcggtca
    25621 ggtcgcggca cacgaagcga tggcgctggg gattgagaaa gcgcatcagc acggtattgc
    25681 tgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg cggagcagtg
    25741 ttcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc caatggtcgc
    25801 gccgttccac ggtcgcgaca gccgctttgg caccaatccc ttctgtgtgg ttttccctcg
    25861 taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat ttggcaaaac
    25921 ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg acgttaacgg
    25981 cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttggggtcgc tgttgacctt
    26041 tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg gggcgctttc
    26101 tggcggtaaa acgacgcatc aggaaacgtt gcaaaccagc cccgatgcca ttcttaactg
    26161 catgaccact atcatcatca acccggaact gttcggcgcg ccggattgta acgcgcagac
    26221 cgaagccttt gccgagtggg tgaaagcctc gccgcatgat gacgataagc cgattttgct
    26281 accgggcgag tgggaagtga acacgcgtcg cgaacggcag gagcagggga ttccattgga
    26341 tgcgggaagc tggcaggcca tttgtgatgc ggcgcggcag attggtatgt cggaagagac
    26401 gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag tggcggacgg
    26461 gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt cggcaaacag
    26521 acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac ggttcggatt
    26581 ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg atgcggcata
    26641 aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt aatttattgt
    26701 tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgccgct ttctcagcca
    26761 gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg ccgattttat
    26821 tcatgttctg tgcctgggat gccgttaccg gttccgccgc gaacacgcca aatgacagag
    26881 ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat ctctcgcagg
    26941 tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag
    27001 agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata tctaacatac
    27061 tgataaacca tttatttgtt gtacttatgg acttgtttta tgccgcgtgg cagtcacagc
    27121 agcgttaaag gcacggaatg ccacgcaaaa tacaaattat actcgctatg catctcgcca
    27181 gttgtagtta acgtaactgt ctgtctttcg aacctctgcg attatatcct gtatacgctg
    27241 gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca ggaatggctt
    27301 cccgacgggc ttgcgggatg ggggcaccgc actcttcaca ttcatacagg ctttcgccac
    27361 gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg atctgttcgt
    27421 tgacggcgtc atcgttagcc caagcggatg ccatatcgtc ctccccatat caatgcttgt
    27481 acagttaagt gtagctaata tagggacgaa cctgggcagt tcaagcgtca gatctccgac
    27541 cattcccgca gcagattatg ataaagattg agcagcgaca ggatctcttc attttcacca
    27601 tagcggcttt tcagcgactg aatatttttg tccagttcaa acagcatggc gcgcttttta
    27661 tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc gcgggttacg
    27721 ggtgtcacgc aatgcaggct gctggaggga tacaacacca gatcacctgc cgggagtttt
    27781 acccgatgtt gtccaaaggt gtcattaacg accagttcgc cgccgtcgta gctttgtgga
    27841 tcgcttaaaa acagcgtggc agaaaggtca gtgcgcatcc agccgttttg tggatggctg
    27901 cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg attaaacagc
    27961 ggcgtggaaa gggtacgcgg caaggcagcg gcaaagaaca atgcatgttg gttaaccgcg
    28021 ttcagcacct cattttgcag tgcggcgtat aacgcgctgc gggtgtctac ttgctgattg
    28081 ttcttaactt gtgcaccttg tgctccggtg gtgacgcgtc catccaccca ttcggcttgt
    28141 tccagttgtt cgcgaaaatg ggcgacgtcc tgtggcgata acacgccggg aatgtggtac
    28201 atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag aaatgcatat
    28261 tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg ctcttgttga
    28321 ttgaggcgac gtaatcggta tcaaacaggt tgtaaacgtt tagctggaag tcgagattgc
    28381 gattaacccg ataccccagt ttggcatcgg cgacccagta accttctgta aatgctggcg
    28441 ttcccaccgc gccgtctgaa cctttatgta tactgccgat atagcgtgcg cccgcgccaa
    28501 cagagatatc atcggttgcc tgatattggc tccataaggt gaaggcgtgc tccggggtat
    28561 acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg gttgcttttt
    28621 gctgggtata gccgccaatc acctgccacg cgggagtgat attccctgcc acggatatct
    28681 catagccttc gacgcgtttc ttaccgtatt gcgagtaagt cccgtcatca ttttgctcaa
    28741 cttcattttc gatatcagta cggaacagcg cggcggtgag caacaggcgt ttatccagaa
    28801 cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt
    28861 tggcactgtt accactgcca gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct
    28921 gggaaacggc atagttaata tagacattgc cgttttccgt caggtggtac agcgccccgg
    28981 ctttccagtt caccagattg cccgacttgg cggtgtcgac ggtggtgact ggagaacctt
    29041 ttgccacacc agctgggcag gtgatggcac cgcgtccgct gccgccgcag gcggtggcac
    29101 tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca aaatcacggg
    29161 tgatttgcag cgtatcgaag gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg
    29221 cgccgttgcg cgtcaggccg ccgggatgaa ttctgctgtc aggatgataa atatttaccg
    29281 ccggtaacgt caccggatta acgccatagt tagtctgcgt ttcacgggta aattccacgc
    29341 cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg ttggtctggt
    29401 tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgactag gtccagctat
    29461 tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc gtcatcaggt
    29521 aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta tcgttgatgt
    29581 cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc
    29641 cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgct cccgcagatg
    29701 gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctgag
    29761 tgacatgcag ataattaaga tacaaacgat ttgctgtacc aaggccaaaa gcgacagaag
    29821 gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgg gttttttcgc
    29881 ccatcacatt caggcgcacc gcggtagtat caccaatgac ctgattgacg tccagcgtgc
    29941 cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg
    30001 gctgcttgct gatcatattg atcgagcctg tcggtgcgct gcgcccgtag tcggtgccgg
    30061 acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag acgctgccga
    30121 tatcgcgaat accatcaata taaatactgt tagaggtatc ggcaccacgc atataaatgg
    30181 cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg c
//
LOCUS       DADLCQ010000044        29634 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000044 DADLCQ010000000
VERSION     DADLCQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.044"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..186)
                     /locus_tag="JQG95_001784"
     CDS             complement(<1..186)
                     /locus_tag="JQG95_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY5888358.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQG95_001785"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="JQG95_001785"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY5888359.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="JQG95_001786"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="JQG95_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY5888360.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="JQG95_001787"
     CDS             3863..4144
                     /locus_tag="JQG95_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY5888361.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4437..4961
                     /locus_tag="JQG95_001788"
     CDS             4437..4961
                     /locus_tag="JQG95_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5888362.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            5042..5716
                     /locus_tag="JQG95_001789"
     CDS             5042..5716
                     /locus_tag="JQG95_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY5888363.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5732..8212
                     /locus_tag="JQG95_001790"
                     /pseudo
     CDS             5732..8212
                     /locus_tag="JQG95_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            8228..9261
                     /locus_tag="JQG95_001791"
                     /pseudo
     CDS             8228..9261
                     /locus_tag="JQG95_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="JQG95_001792"
     CDS             complement(9343..9681)
                     /gene="rcnB"
                     /locus_tag="JQG95_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY5888364.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9900..10748)
                     /locus_tag="JQG95_001793"
                     /pseudo
     CDS             complement(9900..10748)
                     /locus_tag="JQG95_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            10870..11142
                     /gene="rcnR"
                     /locus_tag="JQG95_001794"
     CDS             10870..11142
                     /gene="rcnR"
                     /locus_tag="JQG95_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY5888365.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11234..11330
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11365..12153
                     /gene="thiM"
                     /locus_tag="JQG95_001795"
     CDS             11365..12153
                     /gene="thiM"
                     /locus_tag="JQG95_001795"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY5888366.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12150..12950
                     /gene="thiD"
                     /locus_tag="JQG95_001796"
     CDS             12150..12950
                     /gene="thiD"
                     /locus_tag="JQG95_001796"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY5888367.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13015..13836
                     /locus_tag="JQG95_001797"
     CDS             13015..13836
                     /locus_tag="JQG95_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY5888368.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVDERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            13884..14630
                     /locus_tag="JQG95_001798"
     CDS             13884..14630
                     /locus_tag="JQG95_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5888369.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGGLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLCGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14604..15569)
                     /locus_tag="JQG95_001799"
     CDS             complement(14604..15569)
                     /locus_tag="JQG95_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY5888370.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15566..16569)
                     /locus_tag="JQG95_001800"
                     /pseudo
     CDS             complement(15566..16569)
                     /locus_tag="JQG95_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16566..17843)
                     /locus_tag="JQG95_001801"
     CDS             complement(16566..17843)
                     /locus_tag="JQG95_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888371.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            18100..19152
                     /gene="fbaB"
                     /locus_tag="JQG95_001802"
     CDS             18100..19152
                     /gene="fbaB"
                     /locus_tag="JQG95_001802"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY5888372.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            19460..20314
                     /gene="gatY"
                     /locus_tag="JQG95_001803"
     CDS             19460..20314
                     /gene="gatY"
                     /locus_tag="JQG95_001803"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY5888373.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            20343..21605
                     /gene="gatZ"
                     /locus_tag="JQG95_001804"
     CDS             20343..21605
                     /gene="gatZ"
                     /locus_tag="JQG95_001804"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY5888374.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            21615..22067
                     /gene="gatA"
                     /locus_tag="JQG95_001805"
     CDS             21615..22067
                     /gene="gatA"
                     /locus_tag="JQG95_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY5888375.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            22098..22382
                     /gene="gatB"
                     /locus_tag="JQG95_001806"
     CDS             22098..22382
                     /gene="gatB"
                     /locus_tag="JQG95_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY5888376.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            22386..23741
                     /locus_tag="JQG95_001807"
     CDS             22386..23741
                     /locus_tag="JQG95_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY5888377.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     CDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            23789..24829
                     /gene="gatD"
                     /locus_tag="JQG95_001808"
     CDS             23789..24829
                     /gene="gatD"
                     /locus_tag="JQG95_001808"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY5888378.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            24929..25708
                     /locus_tag="JQG95_001809"
     CDS             24929..25708
                     /locus_tag="JQG95_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5888379.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="JQG95_001810"
     CDS             complement(25790..26689)
                     /gene="yegS"
                     /locus_tag="JQG95_001810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY5888380.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            27095..27412
                     /locus_tag="JQG95_001811"
     CDS             27095..27412
                     /locus_tag="JQG95_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888381.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(27754..29115)
                     /locus_tag="JQG95_001812"
     CDS             complement(27754..29115)
                     /locus_tag="JQG95_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY5888382.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29262..29593)
                     /locus_tag="JQG95_001813"
                     /pseudo
     CDS             complement(29262..29593)
                     /locus_tag="JQG95_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
BASE COUNT         7699 a         6878 c         7480 g         7577 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctgta attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtaccg gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gagcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aaatcattta tataaatgat
     4201 ttcgtctttt ttattgatat caacaatacc atttacataa atctacatta attaatagat
     4261 gtgataaata tttaatattc ttaagtattt gttgtattta tattgcattg gttaatttgt
     4321 tttaaatcaa taaaacaata tgtttacatt gcggcagctt ttttgatgtg agaaatttgt
     4381 ttccaagaga tacagactct taacaaccaa aaataaattt tattaaggaa catattatga
     4441 aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct ggagtatttg
     4501 ctgcagacgt tgataccgga acattaacca ttaaaggaaa tattgcagaa tctccgtgta
     4561 aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca accactgtct
     4621 ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc agcagcatgt
     4681 taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatc acaaacgaca
     4741 aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtaggt gataacagcg
     4801 atgtcctaga tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat
     4861 atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg
     4921 atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa ttgcaagttt
     4981 ctccaggtaa taaacatggc tgctatcccc atggcggctt tttaatttaa gaggcattaa
     5041 gatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat
     5101 tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt
     5161 gatgaaccag ggaaatcgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc
     5221 gttaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg
     5281 ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa
     5341 agaaagcatt ttttatctga atgttctgta tattccacca aatagtccag agcaagaagg
     5401 caagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg
     5461 tattgctccg gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg
     5521 attggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa
     5581 tgttaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga gtgttaatat
     5641 caaaagtaat aatgcaaata actggtatct gaccattatc aatgaccacg gcaactatat
     5701 tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc
     5761 aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca
     5821 ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca
     5881 acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc gcgggaaata
     5941 tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg
     6001 gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact
     6061 tgttcagggt gggagctata gctgggatat cggggttttt cgtctcgatt tcagtgtccc
     6121 gcaggcttgg gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg
     6181 tattaatgcg ttttatactt cttattatgt gagtcagtat tacagcgact ataaagcgtc
     6241 gggtaacaac aagagtacat atgtacgttt taacagcggg ttaaatttac tggagtggca
     6301 actgcattct gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa
     6361 taccctgtat ctggaacgtg gatttgccca atttctcggc acgcttcgcg tgggtgatat
     6421 gtacacatca agcgatattt ttgattctgt tcgcttcagc ggtgtgcggt tgtttcgtga
     6481 tatgcagatg ttgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca
     6541 gagtaacgcg ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc
     6601 tcctggcccg tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt
     6661 tagcgtgaaa gaggcggacg gctcggtaac cacctatctg gtgccttatg cagcggtgcc
     6721 aaatatgctg caacccggcg tgtcgaaata tgattttgcg gcgggccgta gccatattga
     6781 aggggcgagc aagcaaagtg attttgtcca ggcaggttat cagtatggtt ttaataactt
     6841 attgacgctg tatggcggca cgatggttgc taataattac tatgcgttta ccctctgaac
     6901 gggttggaac acgcgcattg gcgcaatttc cgtcgatgcc acgaagtcgc atagtaaaca
     6961 agacaacggc gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag
     7021 ccaaacgtcg acgcgttttg gtctggcggc ctggcgttat tcgtcgcgtg attaccggac
     7081 atttaacgat cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatat
     7141 ctatgacatt gccgattatt accagaacga ttttggccgt aaaaatagct tttctgccaa
     7201 tatgagccag tcattgccag aaggctgggg ttctgtgtcc ttaagtacgt tatggcgaga
     7261 ttactggggg cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg
     7321 gcggataagc tataccctcg cggcaagcca ggcgtatggc gagaatcatc atgaagagaa
     7381 acgttttaat atttttatat caattccctg tgattggggt gatgacgtta cgacgcctcg
     7441 tcggcaaata tatatgtcta actcaacgac gtttgatgat cagggttttg cctcaaataa
     7501 tacgggatta tcaggaacag tagggagtcg ggatcagttc aattatggtg tcaacctgag
     7561 tcatcaacat cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt
     7621 tgcgacagtg aatggcagtt atagtcagtc gagtacttat cgacagactg gagccagtgt
     7681 ttcagggggc attgtcgcct ggtcgggtgg cgttaatctg gcgaaccgtc tttccgaaac
     7741 gtttgctgtg atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg
     7801 cacaacaaac cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca
     7861 cctgatgctg gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat
     7921 tgccgcccct tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc
     7981 ctggtttata aaagcgttaa gagcggatgg tcaaccatta acgtttggtt atgaagtcaa
     8041 tgatatccat ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac
     8101 caatgaaata ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac
     8161 aatcaccttc ggtaaagaga ttgatgaaag tagaaattat atttgccagt aatagtcagg
     8221 tggttctatg gaaattcgca taatgctatt tatattaatg atgatggtta tgcctgtgag
     8281 ctatgcggca tgttatagtg agttatctgt tcagcacaat ttggttgttc aggggaattt
     8341 tgcacttact caaacacaaa tagcgacata tgagcataat tttaatgatt cgtcatgcgt
     8401 gagtacaaat actatcaccc ctatgagccc atcggatatt attgttggac tttataacga
     8461 taccattaaa ttaaatttac attttgaatg gaccaataaa aacaacatca cgttgtcaaa
     8521 taatcagagc agtttcacca gtggttattc ggtaacggtg acacctgcgg ccagtaatgc
     8581 aaaagtgaat atttctgcgg ggggcggtgg ttcagtgatg attaatggtg ttgcgacatt
     8641 atccagtgct tcatcatcga cacgcgggag tgccgcagta caatttctac tgtgtttatt
     8701 aggtggcaag tcatggggtg catgtgtaaa tagctacaga aatgcattgg cacaaaatgc
     8761 aggtgtctat tccttcaatc tgacattgtc atacaacccg ataaccacaa cctgcaaaca
     8821 ggacgattta ttaattactt tagacagtat tcccgtttca caattaccag ccacaggtaa
     8881 caaagcaaca ataaatagta aaaaaaggga tattattctg cgttgtaaaa atttattagg
     8941 tcaacaaaat caaacatcac ggaaaatgca ggtgtattta tcaagttctg acttgttaac
     9001 caacagcaac acaatactga aaggtgcgga agataatgcg taggatttat tcttgaaagt
     9061 aatggttcgc cagtcacact tttaaatatc actaacagca gtaaaggata cacaaattta
     9121 aaagaaattg cggcaaagtc aaaacttaca gatacaacgg tttcaattcc gataacagcc
     9181 agttactacg tctacgatac aaacaaagtt aaatctggcg cactggaggc aaccgcatta
     9241 atcaacgtga aatacgacta ataacaagca aaacgagcaa gtggctaata ataaaaaata
     9301 acaaggtgtc tgcatttccc cctcatgatg agggggcttt ttttagcgat gataaaaaat
     9361 ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg ctgatcaaca cgtacgcgcc
     9421 acccatatag gtccagtgcg tcccggcatc aggcgcgggc aggttacgta actgccactg
     9481 cttaatgttg tactcatcgg tgcggtacat ctctgggacg gtatcacctg gtttgaaatg
     9541 cttaaagtca gcaatgaaac tacttaactc atacttagaa attcccgagg tattggtcga
     9601 acccgcggtg gctggtgcgg cccaggcggc actggtaacc agcaaaagca cacccagcaa
     9661 catcttattt ttaatggtca taaactctcc actttgggga tctgtaatat cagtcgcata
     9721 tctttcctgt catacaatat aactgacaag cgatattttg ttagtgatcc catgatttcg
     9781 taaaaagtgt aaatgaaagg atgccctaaa agcatcctta gcgagcagag agtcagcgga
     9841 aatagctgat ttaagaatca tcctgggctg aagataacat gcaagcctaa aggattgagt
     9901 tatcgcatta tgcccatgaa gccatgtacg cccatataca caccgactaa gccaatcaac
     9961 agactggaaa aatagggggc gcgtttagcg agagtgttaa atccgctcca gcgttttgcg
    10021 acctgctgaa cgctgattgc tgcgccaacg cctacggtga caagcgttaa cgccaggcca
    10081 atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg ctttcaactg aatgcatatc
    10141 aacagcacgg taattgctgc cgggcagggg ataaagccac cggttaagcc aaataacaaa
    10201 atttgccagt tgatgacctc tctaccatca aagcgtcgtt taatgtcatt ggcatgggct
    10261 cgtgcatggg catcctgata ctcgccatat tcgtgatggt gatgatgtcc ttggtcgtgg
    10321 tgatgttcgt ggtcttgatg atgttcgtga tcgtgatgat ggtgatgatg ctcataatca
    10381 tgcccgtgca tattctccag ccagttgcgt tcgccgcgct aggtacgcca gaacatccag
    10441 aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc tgctgattga
    10501 gcagtaaagc gcttactgat caccatcccg ccaaaggcaa tcaaccagac caccgcggta
    10561 tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt taatggtgcc tttgatggcg
    10621 atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg aagcgcacca
    10681 agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag agttgtaaat
    10741 tcggtcatga taataattct tagtattaat tcggcaatct gattctactc ccccccagta
    10801 cctgatacta cccccccagt agattaatag tgctatgatt tttcatattc ttgttaacca
    10861 ggtgttgcca tgtctcatac aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag
    10921 attcagggcc aggtcgtggc gctcaagaaa atgctcgacg agccgcacga atgcgctgca
    10981 gttttacaac agattgctgc tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt
    11041 aaaggtcatc tgacggaaca catcgttcac cagggggatg agctaaaacg tgaagaagat
    11101 ctggatgtcg ttctgaaggt gctggattca tatatcaaat aatttattaa cgcgattgta
    11161 aaactgccgt ttttcctcgt ttacaacggg ggcgctggac attaccatcc tcctctgcga
    11221 tttatcatcg caaccaaacg actcggggtg cccttctgcg tgaaggctga gaaatacccg
    11281 tatcacctga tctggataat gccagcgtag ggaagtcacg gaccaccagg tcattgcttc
    11341 ttcacgttat ggcaggagca aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg
    11401 cacgcgttac acctttttca ccaacattcc cctcttgtgc actgcatgac caatgatgtg
    11461 gtgcaaacct ttaccgccaa taccttgctg gcgctcggtg catcgccagc gatggttatc
    11521 gaaaccgaag aggccagtca gtttgcggct atcgccagtg ccttgttgat taacgttggc
    11581 acactgacgc agccacgcgc acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct
    11641 caaacacgct ggacgcttga tccagtagcg gttggtgcgc tcgattatcg ccgccatttt
    11701 tgtcatgaac ttttatcttt taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg
    11761 gcattagctg gcattgctaa tggcggacgg ggagtggata ccactgacgc cgcagctaac
    11821 gcgatacccg ctgcacaaac actggcacgg gaaactggcg caatcgtcgt ggtcactggc
    11881 gaggtggatt atgttaccga tggacatcgt atcattggta ttcacggtgg cgatccgtta
    11941 atgaccaaag tggtaggaac tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg
    12001 ttaccaggcg atacgctgga aaatgtcgca tctgcctgtc actggatgaa acaagccgga
    12061 gaacgcgcag tcgccagaag cgaggggcca ggcagttttg ttccacattt ccttgatgcg
    12121 ctctggcaat tgacgcagga ggtgcaggca tgaaacgaat taacgctctg acgattgccg
    12181 gtactgatcc gagtggtggt gcggggattc aggccgatct taaaaccttc tcggcacttg
    12241 gcgcttatgg ttgctcagtt attactgcac tggtggcgca aaatacccgt ggcgtacagt
    12301 cggtgtatcg cattgagcct gattttgtcg ccgcccagct cgattcggtg ttcagcgatg
    12361 tgcgaatcga taccactaaa atcggtatgt tggcggaaac cgatattgtt gaagcggtgg
    12421 cagaacggtt gcaacgttat cagatccaaa acgtggtact cgacaccgtt atgctggcaa
    12481 aaagcggcga cccgctgctt tcaccttcgg cggttgctac gctgcgcagt cgattattgc
    12541 cacaggtttc attaataacg ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac
    12601 acgcgcgcac cgaacaggaa atgctggaac aagggcgatc gctgttggcg atgggctgtg
    12661 gcgcagtgct aatgaaaggt ggtcatctgg atgatgagca aagcccggac tggctgttta
    12721 cccgcgaggg tgaacaacgg tttaccgcac cgcgcattat gaccaaaaac acccacggca
    12781 ctggttgtac actctctgcg gcgttggctg cactacgccc gcgccataca aactgggctg
    12841 acaccgtaca ggaggcaaaa atctggcttt catcggcgtt agcccaggcc gacacgctgg
    12901 aagttggtca cggtattggt ccggttcacc acttccacgc ctggtggtga gcaccgtact
    12961 atagctgctt tggttattgt cagcctttgg cactgacatt cagggatgaa accaatgcag
    13021 ctaagaatta ccagtcgaaa aaaactcacc gctctattat gcgctcttgg gctaatttcc
    13081 atcgttgcaa tttatccgcg tcagacggtg aattttttct attcgacagc agttcagatt
    13141 acagactaca tccacttcta cggttatcgc ccggttaaat ctttcgctat tcgcattcct
    13201 gccagttaca ccattcacgg attagatgtt tcgcgctggc aggagcgtat cgactggcag
    13261 cgggtggcaa aaatgcgcga caacgatatc cgcttacagt ttgcttttat taaggctacg
    13321 gaaggcgaaa agctggtgga cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat
    13381 ggcctgctgc gcggggcgta tcattatttc tccccgtcgg tatccgcttc agttcaggcg
    13441 cgattatttc tgcaaacggt ggatttctca caaggcgatt tccctgccgt gctggacgta
    13501 gacgaacggg gaaaattatc ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa
    13561 atggtcgaaa aacgcacggg aaaaaagccg attatttact caggagccgt tttttatcac
    13621 accaatctgg cgggctattt caatgaatat ccgtggtggg tggctcacta ttatcaacgt
    13681 cgtccggaca atgacggcat ggcctggcgc ttctggcagc attccgaccg tggacaggta
    13741 gatggcatca atggtccggt ggattttaat gtgtttaatg gcacggggat gagttgcagg
    13801 cattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata ctcccaccag
    13861 actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt gcccaactga
    13921 acgagcgtat tttagcggcg gataacacgc cgctttatat taagtttgcc gaaacggtaa
    13981 aaaatgccgt gcgcagcggg gggctggagc atggcaatat tttgcccggt gagcgtgatt
    14041 taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag
    14101 aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac atcttcgaat
    14161 actcgttgaa agaagcgaga ggtttttctc agcaggtggt attgtgcggg aaaaagcccg
    14221 atacgctatg ggttaataag cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg
    14281 cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag
    14341 cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt gatgccatcg
    14401 gaatttctct ttatgactat ttccgcagcc agcacattta cccacaacgt acgcgttccc
    14461 gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta gatagcaaaa
    14521 taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct attgagtaca
    14581 gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc
    14641 gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc attgcccagt
    14701 aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc acctgcatga
    14761 ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat gcagccagaa
    14821 gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa cggcgcggca
    14881 aatttctcct gccattgctt gccaagtgtt gttatctctg cggataaagc aaaacgttcg
    14941 gcggcaatct cagcctcttg acgattgagt gacactaaag gtcgacaggc catgatccgc
    15001 gccagtaatg cgtccgggat atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg
    15061 tcttgcagct tttccagcca ttccactaac aattcgccgc agggcgaggc cagttgataa
    15121 ccggaaaaat agagcaggtt gccaggcgca acggttaatc gtgccaacca ttggcgattc
    15181 cattgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt
    15241 gccagacacc agccgttatc gctttcggcg ttatcgatca ggctgattaa cccctctttc
    15301 gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa ggcattaccc
    15361 gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc gccaacgtta
    15421 acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata agcgtcggcg
    15481 atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt taattcaggc
    15541 agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga tatttcgcca
    15601 gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagctcc gcctttaacg
    15661 caggatcgac agcgttaatg ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta
    15721 tggtgtctgt gtcgccgcca agattggcgc acaggacggc gcagcggttt gggtcggttt
    15781 gtgccagttc aactagcgca atggcgcacg gaacggactc aatagtgctg gtgcctgcgc
    15841 caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcatta cgcacaattt
    15901 tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct
    15961 gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa
    16021 tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga tcggatttat
    16081 gggtcgggct ggacgccagc gccacatcat caataaagga atcaacatca cgcgctggaa
    16141 gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca
    16201 gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta
    16261 gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc gagaatatta cgcccaatca
    16321 gattcggatc gatcttgcct tcacattcca gtaacgcatc cgccagacac aggccatcga
    16381 ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc gcgttattct cctttggtcc
    16441 aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg cggctgcgtg gccaaagctc
    16501 ggagggcatc cccatcgcat cccctaacgc ctgcccataa agagcaccga gaatacgttc
    16561 tgttttcatt taacttcccc ttgtgtcaac gcaatatctc gatcatcgac cttgatagcc
    16621 gtaatttcgt tgtcggattc gcggaaaaaa atcatgaaca gcacggcgat aatggcaatc
    16681 atcaccgcgc cgaaagtcca catcccggac cagttgaaag tcagtccgtt taccggttcc
    16741 tgataagcga acatcttttc catcatcaca ccgccaagac gatagccgag caaactgccg
    16801 aagccctggc agcagagcgt gatcagcccc tgcgcagcgg tacgcatatg cacgggggct
    16861 tttttatcga catagatgta agcggtaacg tagtaaaaat cgtaacttac gccgtgaagc
    16921 aaaataccga ggaacagtaa cgcgtaggtg aaatattcat ccgcactacc gtaaataaag
    16981 aagccatagc agatcgcagc ggtgaccaga ccaagcaata ataccttttt gataccaaag
    17041 cgtttagtga aaaacggcaa tgccagcata aagaagattt cagagaactg gccgagcgtc
    17101 atccagccgg tggcgttttt catgccaact tcggtcagat aaccgttggc aaagatgtaa
    17161 tagaacgcca gtggcatcgc aaacaggaat gaacagaaga aaaagacgag gaagttttta
    17221 tcgcgcagca ggatcagcgc atccaggccg agcatgactt taatgtccat tttgccggtg
    17281 ctttttggcg gcgtgtcggg caggaaaaac gcaaacacac cgagcagagc agaacttccg
    17341 gcggtaatca gcagcgggat gttagtcggt gagatatcgg cataccccag catttgcggc
    17401 aagaaaccac atgccagacc ggaggcaatc cagccgatag tgcccatcac acgaatgcgt
    17461 gggaaatcac gctccacatc cggcacgttg gcaaaagcga tgctgttagt cagcgcaatg
    17521 gtcggcatat aggttagcga gtaggccagc agtaacggaa agaacccggc aaaagtggtc
    17581 tgttgcgcag cgaaatacat cagcacagca ccagcgaaca tcaataccgc cagcactttc
    17641 tgcgccgaga aaaagcggtc agtgatggag ccaaccagaa ttggcgacag gatcgccgca
    17701 atggctgtgc aggcatacga ccagccaatt tctccggcac taaaaccgct tttacttaac
    17761 cacaaccaca atggcacaaa ccacgcgccc cagataaacc attcaacaaa catcatgaac
    17821 gacagctttg ctgttgtttt catcttttaa ttccttcatg acaggtaatg taaggtacgc
    17881 gatggcaata ccacataata atgccttttg aatacctttg cggtgaaaac ttgaccgcgc
    17941 taacattttt tctgatgaat cgagccaaca gaaaacgctg aaaaaacatc caaaagatgg
    18001 aaaaactcgc ccgtaacgac taggattatg attgcccgca aaaggcgggc caggacaatc
    18061 catagccgat atccaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    18121 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    18181 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    18241 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    18301 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    18361 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    18421 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    18481 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    18541 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    18601 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    18661 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    18721 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    18781 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    18841 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    18901 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    18961 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    19021 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    19081 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    19141 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    19201 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    19261 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    19321 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    19381 ttgtgatcat tatctcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    19441 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    19501 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    19561 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    19621 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    19681 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    19741 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    19801 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    19861 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    19921 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    19981 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    20041 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    20101 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    20161 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    20221 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    20281 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    20341 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    20401 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    20461 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    20521 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    20581 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    20641 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    20701 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    20761 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    20821 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    20881 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    20941 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    21001 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    21061 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    21121 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    21181 tcggtcccgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    21241 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    21301 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    21361 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    21421 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    21481 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    21541 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    21601 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    21661 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    21721 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    21781 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    21841 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    21901 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    21961 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    22021 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    22081 aataaaaagt actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    22141 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    22201 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    22261 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    22321 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacaggggt
    22381 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    22441 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    22501 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    22561 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    22621 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    22681 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    22741 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    22801 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    22861 agctctgcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    22921 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    22981 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    23041 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    23101 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    23161 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    23221 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    23281 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    23341 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    23401 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    23461 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    23521 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    23581 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    23641 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    23701 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    23761 ttatccccct taacaatcag gagtttttat gaaatcagta gtgaatgata ctgatggtat
    23821 cgtgcgcgtt gcaaaaagcg tcattcctga aattaaacat caggatgagg tgcgggtaaa
    23881 aattgccagc tcgggcttat gtggttccga tttacccagg atatttaaaa atggtgcaca
    23941 ttattatcca ataacgttag gccatgaatt tagcggctat attgatgcgg tgggatccgg
    24001 tgttgatgat ttacaccctg gcgatgcggt tgcctgtgtg ccgttattac cctgttttac
    24061 ttgtccagag tgtttgaaag ggttttattc ccagtgcgca aaatatgatt ttattggctc
    24121 gcggcgtgat ggtggatttg ctgaatatat tgtcgttaag cgaaaaaatg tctttgctct
    24181 acccacggat atgcctattg aggatggggc ttttattgag ccgattaccg ttggcctgca
    24241 tgcttttcat ttagcgcaag gttgtgagaa taaaaacgtt attattattg gtgccggaac
    24301 cattggcctg ctggctattc agtgcgctgt cgcgctggga gcaaagagtg tgacggcgat
    24361 cgacattagc tcagaaaaac tggcactggc aaaatctttc ggtgcgatgc aaacatttaa
    24421 cagtagcgaa atgagcgcgc cgcaaatgca gagcgtttta cgcgaactgc gctttaatca
    24481 gcttatcctc gagacggctg gcgtaccgca aactgtcgaa ctggcggtag agattgccgg
    24541 tcctcatgcc caactggcgc tggtgggcac gttgcatcag gatctgcatt taacatcggc
    24601 aacgtttggc aaaatattgc gtaaagagct gacggttatc ggcagttgga tgaactattc
    24661 cagcccttgg ccggggcagg agtgggaaac ggcgagccgg ttgctgacag aacgtaagtt
    24721 aagcctggag ccattaatcg ctcaccgtgg aagctttgaa agcttcaccc aggcggtgcg
    24781 tgacatcgct cgtaatgcca tgccgggcaa agtgttgctc attccatgaa accgcgggcc
    24841 agcgtgatgc tggcccggta ttgtgcaaaa cagatcattc accaatggtc ccccttcgtt
    24901 tacactagcc acaattgaat atgggtaaat gacgatgaat tcattcgagc gaaggaataa
    24961 gatcatccaa ttagtgaatg aacagggaac cgtgcttgtt caggatctgg cgggagtatt
    25021 tgctgcctcg gaagcgacaa tccgtgccga tttgcgcttt ctcgaacaaa aaggcgtggt
    25081 tacgcgcttt catggcggtg cggcgaaaat aatgtccggt aatagtgaaa ccgagaccca
    25141 ggaagtcggg tttaaagagc gatttcagct cgccagcgcg ccaaaaaaca gaatagcgca
    25201 ggcggcagtc aaaatgatcc atgaagggat gaccgttatt ctcgacagcg gaagtaccac
    25261 aatgcttatc gccgaaggat taatgaccgc taaaaatatc acggtgatta ccaacagtct
    25321 cccggcagcg tttgcccttt ccgaaaataa agacattact ctggtcgtct gtggtggcac
    25381 ggtccgccat aaaacgcgct cgatgcatgg ttccattgcc gagcgttcat tgcaggatat
    25441 taatgccgat ttaatgtttg tcggtgctga tggtattgat gcggttaatg gtattacgac
    25501 ctttaatgaa ggttattcga ttagcggggc gatggtcacc gccgcaaata aagtgattgc
    25561 tgttctcgat tcatcgaaat ttaatcgtcg cggttttaat caggtactgc caatcgaaaa
    25621 aattgacatt attgttaccg atgatgctgt gtcagaggtg gataaattgg cattgcagaa
    25681 gacacgggta aaattaatta cggtgtagta ttgttctgta tgccggataa ggtgtttaca
    25741 ccgcatccgg cattacggat tctccctggt tcaagagaga atagtttgat taacgcaata
    25801 atggacaatc tggtggtaat cgacaacgca acgccgccgg aagtatttca atatggaagt
    25861 tttgcccact caacggttcg ccatcaagat taaaagtgat ttcgtgtggg gcttgtatat
    25921 caaaccacga cgaagcgcct tcgataatat tcgggttatc ttcgtcagat tttaaggttg
    25981 atacgagagc cggaagaatt tcatcgccgg taaaaatgcg cagttgcagc aagccatcgt
    26041 taattaacgc gttcgggcac aattgttgac cgccaccggc ctgacgcccg ttaccaatac
    26101 caatgaccag ggcgtcacct tgccagtgaa agttttcacc gcggatttca caacggtccg
    26161 gttgcagagt atccatgcgc attaagccat gaatgatgta agagacgcca cccagcgcgg
    26221 cttttaattt ttccggcgtt tctgtggtaa tacgcgtccc aaatccgcct gtcgccatat
    26281 taataaaaca ggtttgtttg ttgacctgcg ccatatctat cgcaatggca ttaccggcaa
    26341 ttgccagttt cagcgcctta tccagtgcct caggaatccc tacactggtg gcaaaatcat
    26401 tggcggttcc taatggcaaa attcccagcg cgggtatgtc atccccctca cactgaatca
    26461 acgccgtaga aacttcatta atggtgccat cgccaccacc ggcaatcacc gttgcgacgc
    26521 ccaacttccg ggcctcctct acaaatcgtg cggcatcacc tttctcccag gtgacccgca
    26581 catggatcgt cattccttcc tcacgcaaca gcataattgc ttcgcgcaag ggtagattgt
    26641 cagtactttt accattaaga atcagtaagc tggcgggaaa ttctgccatg atcgtatgtg
    26701 cctttatgat tggtctgtag atagtgtaga gcagaaaaca aaaggtggag tcagaacagg
    26761 atgaaagtcg gaggataaaa agtcagttca agtatggagg attcagtggt taccaccagg
    26821 agcttgcaat ggggacagcg aatactaacg gctgccaccg ataaatttca aaaaagagca
    26881 tatacctaat attcaactaa acagtggcat cttcaatata atatattaaa gcccccatgg
    26941 agttaccctg aagggcctca atgtccgtaa ttcctactta tgtaggaaat gttgtacaga
    27001 gcatttatta taatcctatt caattataat aatcatgcca ttattatatt taaacactag
    27061 agagtgtcgt tggtatttaa tgggggaagg tgagatgaaa aagatagctg ctatatcatt
    27121 aattagtatt tttcttatat ctggttgtgc tgtgcataat gatgaaacaa gtatcggtaa
    27181 atttggtctt gcatataaaa gtaatattca gcgtaaactc gataaccaat actacaccga
    27241 agccgaagct tctttagcca ggggcagaat atctggtgca gaaaatatag taaaaaatga
    27301 tgcagcccat ttctgtgtta ctcagggcaa aaaaatgcag atagttgacc tgaagacaga
    27361 aggtgcagga ttacatggcg tcgctcgtct gacattcaaa tgtggagagt gagaatattt
    27421 ttttgtaagc gtcaaacatg cgcgttctgg ctgtgcgtag ccggaacctg ggcgagcacg
    27481 ataccgttac gtgaaaggca ttgtgctatg aagggggatt ctatcgatgt ggtcaatgga
    27541 agacggttat cagggatcaa tccttcttta cagcttatgt acaaaaaata agcccgtgta
    27601 agggagatta cacaggctaa ggaggtggtt cctggtacag ctagcatttt atggattatg
    27661 tttttcagcg aaacggatga taaccttaat aaatgcagct gtatgtgatc ggtttctaag
    27721 aattttccat ccgggaaaaa taatcgaaat taatcactta ccgtgggggt tacgcgtggt
    27781 ttccccggag aaattacgca ttagcagcgc gtaatttagc tcaagatcct gcgggaccgg
    27841 gagccacaca gtataaccat cgcctggtgc gaccggcata gcttcgcctt tggcgttttc
    27901 catgtgctca agggtaaagt taatgttgcc ttgcggcgtc atcagctcaa gactgtcgcc
    27961 aacggagaat ttatttttca ccgctaccgc tgcgaggtcc cccttgcgct caccggtaaa
    28021 ctcaccaaca aactgctggc ggtcagaaac tgaataaccg tattcgtagt tctgataatc
    28081 gtcgtgagta tgacgacgca ggaaaccttc ggtatagcca cgatgcgcca gaccttccag
    28141 agtttccagc aggctggtat cgaacggttt tcccgcagcg gcgtcatcga tagctttgcg
    28201 gtaaacctgt gcggtgcgtg cgcaatagta gaaagatttg gtacgacctt cgattttcag
    28261 cgaatgcacg cccattttgg tcaggcgttc tacatgggcg atggcgcgca gatctttcga
    28321 gttcatgatg taagtgccgt gctcatcttc aaacgcggtc atatactcgc ccggacgctg
    28381 ggcttcttcg atcataaaca ctttgtcggt tggcgcgccg atacccagcg tcggctcaac
    28441 attttgcacc ggaatcggct cgtacttgtg tacgatgttg ccaacgtcat cttctttccc
    28501 ttcctggacg ttgtactccc agcggcaggc gttggtgcag gtgccctggt tcgggtcgcg
    28561 cttgttgata tagccagaga gcaggcagcg accggagtag gccatgcaca gtgcgccgtg
    28621 aacgaagatc tcgatctcca tatccggcac ctgattatgg atctcttcaa tctcttccag
    28681 cgacagctcg cgagagagga tcacgcgggt cagtcccatt tgctgccaga atttcaccgt
    28741 cgcccagttc acggcgttag cctgcaccga gaggtggatc ggcatttcag ggaagtgctc
    28801 acgcaccagc ataatcagcc ctggatcgga cataatcagc gcatccggcc ccatttccac
    28861 caccggtttc aggtcacgga taaaggtttt cagcttggcg ttgtgcggtg caatgttgac
    28921 cacgacataa aactttttcc ccagcgcgtg ggcttcattg atgccgagct gaagattttc
    28981 gtggttgaat tcgttgttgc gcacacgtag ggagtaacgc ggctggcccg cataaacagc
    29041 atctgcgcca taagcgaaag cgtaacgcat atttttcagc gttcccgccg gggaaaggag
    29101 ttccggttta aacataattc tctcgttctg atgacaggtc agatccgctt cacctgatga
    29161 agcggttagg ggaggccccc actttaaggg cgggcattgt agcgctgtag gggggatctg
    29221 gtaaagcgcc ggacgccaac aacccacgcc cggcatcgta attacgtatt gtctttcacc
    29281 gtctggcttg tgccattggc tttaacggag gcaattccgg tttcacgaga ttgcgcggtg
    29341 gcgtacatct ggctggagcc gataatttga tgattagcgg ctttcagatt gaaatagaat
    29401 ttgccgttgc ttgccgtttt ttctcatagc gctcttcttg cgggctgttg ctacgcaccg
    29461 acgcgatgcc cttttccgca gaggcttttg aggtatacag ctcgctggtg aggatagtct
    29521 caccattgcc cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa
    29581 accaaccagc cataatgact ccttgattga aattgcttat cgaaaacagc atct
//
LOCUS       DADLCQ010000045        29236 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000045 DADLCQ010000000
VERSION     DADLCQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.045"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..218
                     /locus_tag="JQG95_001814"
     CDS             <1..218
                     /locus_tag="JQG95_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5888383.1"
                     /translation="DTAMNFIRQGLGIALQPELTLKSIAGELCSVPHEPTFYRQISLL
                     TKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="JQG95_001815"
     CDS             complement(260..1336)
                     /gene="glpQ"
                     /locus_tag="JQG95_001815"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY5888384.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="JQG95_001816"
     CDS             complement(1341..2699)
                     /gene="glpT"
                     /locus_tag="JQG95_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY5888385.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2972..4600
                     /gene="glpA"
                     /locus_tag="JQG95_001817"
     CDS             2972..4600
                     /gene="glpA"
                     /locus_tag="JQG95_001817"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY5888386.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4590..5849
                     /gene="glpB"
                     /locus_tag="JQG95_001818"
     CDS             4590..5849
                     /gene="glpB"
                     /locus_tag="JQG95_001818"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY5888387.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5846..7036
                     /gene="glpC"
                     /locus_tag="JQG95_001819"
     CDS             5846..7036
                     /gene="glpC"
                     /locus_tag="JQG95_001819"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY5888388.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7229..8179
                     /locus_tag="JQG95_001820"
     CDS             7229..8179
                     /locus_tag="JQG95_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY5888389.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8220..8690)
                     /locus_tag="JQG95_001821"
     CDS             complement(8220..8690)
                     /locus_tag="JQG95_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY5888390.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8745..9947)
                     /locus_tag="JQG95_001822"
     CDS             complement(8745..9947)
                     /locus_tag="JQG95_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY5888391.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(10034..10576)
                     /locus_tag="JQG95_001823"
     CDS             complement(10034..10576)
                     /locus_tag="JQG95_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888392.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            10855..11280
                     /gene="nudI"
                     /locus_tag="JQG95_001824"
     CDS             10855..11280
                     /gene="nudI"
                     /locus_tag="JQG95_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY5888393.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(11319..11921)
                     /locus_tag="JQG95_001825"
     CDS             complement(11319..11921)
                     /locus_tag="JQG95_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY5888394.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            12229..13368
                     /gene="arnB"
                     /locus_tag="JQG95_001826"
     CDS             12229..13368
                     /gene="arnB"
                     /locus_tag="JQG95_001826"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY5888395.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAESGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRTAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            13372..14340
                     /gene="arnC"
                     /locus_tag="JQG95_001827"
     CDS             13372..14340
                     /gene="arnC"
                     /locus_tag="JQG95_001827"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY5888396.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            14340..16322
                     /gene="arnA"
                     /locus_tag="JQG95_001828"
     CDS             14340..16322
                     /gene="arnA"
                     /locus_tag="JQG95_001828"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY5888397.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFLPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSCYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            16319..17209
                     /locus_tag="JQG95_001829"
     CDS             16319..17209
                     /locus_tag="JQG95_001829"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY5888398.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            17209..18861
                     /gene="arnT"
                     /locus_tag="JQG95_001830"
     CDS             17209..18861
                     /gene="arnT"
                     /locus_tag="JQG95_001830"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY5888399.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKTIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            18858..19193
                     /gene="arnE"
                     /locus_tag="JQG95_001831"
     CDS             18858..19193
                     /gene="arnE"
                     /locus_tag="JQG95_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY5888400.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVSQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            19193..19579
                     /gene="arnF"
                     /locus_tag="JQG95_001832"
     CDS             19193..19579
                     /gene="arnF"
                     /locus_tag="JQG95_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY5888401.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="JQG95_001833"
     CDS             complement(19573..19845)
                     /gene="pmrD"
                     /locus_tag="JQG95_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY5888402.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(19950..21305)
                     /gene="menE"
                     /locus_tag="JQG95_001834"
     CDS             complement(19950..21305)
                     /gene="menE"
                     /locus_tag="JQG95_001834"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY5888403.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(21302..22264)
                     /gene="menC"
                     /locus_tag="JQG95_001835"
     CDS             complement(21302..22264)
                     /gene="menC"
                     /locus_tag="JQG95_001835"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY5888404.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(22264..23121)
                     /gene="menB"
                     /locus_tag="JQG95_001836"
     CDS             complement(22264..23121)
                     /gene="menB"
                     /locus_tag="JQG95_001836"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY5888405.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(23136..23894)
                     /gene="menH"
                     /locus_tag="JQG95_001837"
     CDS             complement(23136..23894)
                     /gene="menH"
                     /locus_tag="JQG95_001837"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY5888406.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(23891..25561)
                     /gene="menD"
                     /locus_tag="JQG95_001838"
     CDS             complement(23891..25561)
                     /gene="menD"
                     /locus_tag="JQG95_001838"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY5888407.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(25650..26945)
                     /gene="menF"
                     /locus_tag="JQG95_001839"
     CDS             complement(25650..26945)
                     /gene="menF"
                     /locus_tag="JQG95_001839"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY5888408.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="JQG95_001840"
     CDS             complement(27024..27329)
                     /gene="elaB"
                     /locus_tag="JQG95_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY5888409.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(27384..27845)
                     /locus_tag="JQG95_001841"
     CDS             complement(27384..27845)
                     /locus_tag="JQG95_001841"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5888410.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            27910..28827
                     /gene="rbn"
                     /locus_tag="JQG95_001842"
     CDS             27910..28827
                     /gene="rbn"
                     /locus_tag="JQG95_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY5888411.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            29018..29206
                     /locus_tag="JQG95_001843"
                     /pseudo
     CDS             29018..29206
                     /locus_tag="JQG95_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7087 a         7856 c         7300 g         6993 t
ORIGIN      
        1 cggatacagc gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa
       61 cgctgaaaag cattgcaggt gaattgtgtt ccgttcctca cgaaccaacc ttctatcgac
      121 agatttcgtt gctgactaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa
      181 tgtgcatgga acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga
      241 acgccatcct gcagtaacat tactctttat taagaaattt tactgcctta tcagggaaat
      301 cagtaaacag cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat
      361 caggagtgta ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat
      421 tctgctgggc atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct
      481 caatcaacat atggtaatcc ggaccaatac catctgcata ttccgccacc tgtttcatgg
      541 ctcccggctt aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct
      601 gctgcgtttc attccagtcg gtataggcaa tcagctgtac cagattgagc tccatgccca
      661 ttttgggttc cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat
      721 aaactttatc gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg
      781 caatatcctt cccttcctga tgatggaacc acggcgcttt gatttctgga tagataccga
      841 tatttttccc ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt
      901 gcacccggaa gtcggactta cccattggga aacgccccgg ataagtctgt acttttttac
      961 cgttttcaat atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat
     1021 ctatcgcgta gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag
     1081 taacacgatc gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca
     1141 aatcctgttc cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat
     1201 gctccggcaa atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg
     1261 ccgccattgc actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt
     1321 tcagcttcaa tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga
     1381 cgtttttcgc caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc
     1441 attaccataa agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc
     1501 gccgccaccg aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc
     1561 gcttttttcg gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc
     1621 aggaagccga taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc
     1681 cagtaaacga tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg
     1741 ttaccacgga agactttgtc cgacatccag ccgcacagca gggtgcccgg aatacccgca
     1801 tactcataga agaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc
     1861 agataagtcg gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg
     1921 atggcgatgt accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg
     1981 gtcagctcct gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg
     2041 atcggcggca agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc
     2101 accagaatgg cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc
     2161 atccccagca ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac
     2221 actgacacaa tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac
     2281 ggcggccacc ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca
     2341 atgctcgacg tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc
     2401 aaacctgcgg gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat
     2461 ttcgaaaatc cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag
     2521 aatccctgct caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa
     2581 gccgcatagc caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga
     2641 tcgatctccg cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt
     2701 gatagcctcc gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc
     2761 tgccgtttct tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc
     2821 gcacacatta ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttta
     2881 ctttcgaatc atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa
     2941 atggtaaaaa acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga
     3001 cgtgattatc attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg
     3061 cgggctgcgc gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa
     3121 ccacggcctg ctgcacagcg gtgcgcgcta tgcagtaacc gatgcggaat cggcccgcga
     3181 atgcattagt gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa
     3241 cggcctgttt atcaccctgc cggaagatga cctctcattc caggccactt ttattcgcgc
     3301 ctgcgaagaa gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga
     3361 acctgccgtt aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc
     3421 atttcgtctg accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac
     3481 cgctcatgaa gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg
     3541 taaccatctc accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg
     3601 gatctggggg caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa
     3661 aggatcgctg ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa
     3721 accttccgac gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc
     3781 tttacgtatt gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat
     3841 tctgctgcgt gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc
     3901 ctattctggc gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag
     3961 ccgtggcatc gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat
     4021 caccggtggc aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg
     4081 ccgcaaactg ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca
     4141 agaacccgct gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc
     4201 tgcggtttat cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctacaccg
     4261 tagcctggta tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa
     4321 tttaaacgtt aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg
     4381 ccagggcgaa ctctgcgctt gccgcgctgc cggactgctg caacggttta acgtcacgac
     4441 gtccgcgcaa tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca
     4501 acccatcgcc tggggagacg cactgcgcga aagcgaattt acccgctggg tttatcaggg
     4561 actgtgtggt ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggtg
     4621 gcctcgccgg attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg
     4681 tcacgcgtgg tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc
     4741 tgccagatgg tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg
     4801 cacctgcaca tccttactct cttctcgggc cacaacgcgt gctcgatctc gcttgccagg
     4861 cgcaggcatt aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc
     4921 agcggattac gccgctcggc actttgcgtg caacctggtt aagttcgcca gaagttcccg
     4981 tctggccgct acccgcgaag aagatatgtg tagtggggat aagcgggctg atggatttcc
     5041 aggcgcatct tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa
     5101 tagagctgcc ggaactggat gtcctgcgca ataacgccac cgaatttcgc gcggtgaata
     5161 tcgcccgttt ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg
     5221 tcgccaatac ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac
     5281 tgtggcgttg gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc
     5341 cttccgtgct gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccaaggtg
     5401 gagtgtggat gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg
     5461 aaatctggac ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca
     5521 gcggcagttt ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc
     5581 tcggccttga tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg
     5641 cgccgcaacc gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg
     5701 cagggcaaac cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca
     5761 tcgcccaggg atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga
     5821 ttgcccaacg cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg
     5881 caccgtctgc accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa
     5941 acaagccggg ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc
     6001 gctgaaatat tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat
     6061 tggcgatatt atccagcgcg cgcgggcgaa atatgacacc acgcgcccgt cgctgcgtaa
     6121 ttttgtgttg agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt
     6181 caacaccgct acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga
     6241 tcatcgtcgc acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat
     6301 agcggctcag caagcacaat ataaagacca ggtcgctttc tttcacggct gcttcgttaa
     6361 ctacaaccat ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg
     6421 tgtacaactg ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac
     6481 cgataaagca cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt
     6541 aaaaggcatt ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata
     6601 cccggaagtg ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg
     6661 ctggctgtgg cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa
     6721 agtggtttat cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga
     6781 gctgttgcgt aacatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat
     6841 tgcgggtact tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc
     6901 actgttccgc cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg
     6961 taaatggcag attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc
     7021 ccaggcgctg gcttaaactc ccttctgatg cccggtaagc atgtggttac cgggcatttt
     7081 tgcgtacacg attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa
     7141 cgtctcgcaa aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt
     7201 catcataaat atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga
     7261 tgcggtattt aaaaccttta tattcacacc cgaaaccgca cgggattttc tcgaaataca
     7321 tttaccagaa ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag
     7381 ttttattgaa aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag
     7441 cgacggtgac ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat
     7501 ggcttttcgg ccaatgcgct atgccactgc cgccatgcag agtcacctag ataaaggcta
     7561 tgacagagtt ccgctggtgg tgccattgct gttttatcat ggcgaaacat cgccctaccc
     7621 gtactcactt aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac
     7681 cgaagctttt ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg
     7741 gcgtatagct ctgctggaac tgattcaaaa gcatattcgc gaccacgatt taatcggcat
     7801 ggtcgacagg atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac
     7861 actgtttaat tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattcagga
     7921 gattgccgaa cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg
     7981 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
     8041 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
     8101 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
     8161 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
     8221 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
     8281 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
     8341 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
     8401 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
     8461 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
     8521 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
     8581 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
     8641 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
     8701 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
     8761 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
     8821 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
     8881 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
     8941 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
     9001 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
     9061 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
     9121 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
     9181 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
     9241 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
     9301 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
     9361 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
     9421 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
     9481 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
     9541 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
     9601 cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
     9661 caggcttcat gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
     9721 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
     9781 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
     9841 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
     9901 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
     9961 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    10021 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    10081 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    10141 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    10201 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    10261 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    10321 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    10381 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    10441 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    10501 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    10561 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    10621 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    10681 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    10741 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    10801 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    10861 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    10921 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    10981 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    11041 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    11101 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    11161 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    11221 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    11281 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
    11341 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    11401 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    11461 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    11521 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    11581 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    11641 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    11701 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    11761 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    11821 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    11881 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
    11941 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    12001 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    12061 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    12121 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    12181 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    12241 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    12301 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    12361 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    12421 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    12481 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    12541 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    12601 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
    12661 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    12721 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    12781 acctgtgctg aaagtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    12841 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    12901 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    12961 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    13021 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    13081 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    13141 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    13201 cgcaccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    13261 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    13321 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    13381 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    13441 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    13501 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    13561 gcggagaaca gccatattgt gtctattttg attaaccgca attacgggca acattcagcg
    13621 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    13681 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    13741 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    13801 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    13861 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    13921 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    13981 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    14041 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    14101 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    14161 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    14221 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    14281 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    14341 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    14401 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    14461 tttatggttc ggtggctcat ctagcggcgg aaagagacat tccggtttat gcgccggata
    14521 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    14581 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    14641 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    14701 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    14761 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    14821 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    14881 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    14941 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    15001 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    15061 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    15121 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    15181 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    15241 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    15301 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    15361 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    15421 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    15481 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    15541 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    15601 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    15661 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    15721 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    15781 agggtttaca gttcaccctc ttcctcccgt ttaactggat gggaccacga ctggataacc
    15841 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    15901 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    15961 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    16021 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    16081 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    16141 gcgttgtcga aagtagctgc tactacggca aaggatatca ggacgtagag catcgtaaac
    16201 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    16261 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    16321 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    16381 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    16441 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    16501 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    16561 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    16621 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    16681 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    16741 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    16801 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatac cgttccgtcc
    16861 actgcttgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    16921 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    16981 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    17041 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    17101 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    17161 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    17221 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    17281 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    17341 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa aaccattgcc
    17401 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    17461 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    17521 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    17581 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    17641 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    17701 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    17761 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    17821 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    17881 gcgatagcgc agcgggagcc tgacttctgg cactattttt tctgggttga gcatattcaa
    17941 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    18001 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    18061 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    18121 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    18181 atgctgttgg cgcattacgc gttgctggca gcaaaaaata atcctctggc gctgcggatt
    18241 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    18301 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    18361 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    18421 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    18481 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    18541 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    18601 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    18661 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    18721 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    18781 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    18841 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    18901 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    18961 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg atgctctggc
    19021 tgctggtctc gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    19081 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    19141 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    19201 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    19261 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    19321 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    19381 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    19441 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    19501 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    19561 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    19621 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    19681 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    19741 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    19801 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    19861 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    19921 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    19981 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    20041 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    20101 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    20161 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    20221 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    20281 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    20341 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    20401 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gccgtcggct
    20461 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    20521 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    20581 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    20641 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    20701 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    20761 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    20821 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    20881 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    20941 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    21001 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    21061 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    21121 gtattccacg cccgcaacat cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc
    21181 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    21241 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    21301 atcataacaa ctgctcctgt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    21361 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    21421 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    21481 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    21541 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    21601 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    21661 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    21721 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    21781 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    21841 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    21901 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    21961 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    22021 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    22081 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    22141 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    22201 tgtcgcgcag aaccaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    22261 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    22321 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    22381 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    22441 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    22501 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    22561 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    22621 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    22681 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    22741 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    22801 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    22861 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    22921 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    22981 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    23041 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    23101 tgcttcatca ggataaatca tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga
    23161 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    23221 tggcagtcgg cagccagttc cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat
    23281 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    23341 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    23401 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    23461 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    23521 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gacaataacc
    23581 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    23641 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    23701 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc taggagatca
    23761 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    23821 ccggaaaaac cgtgcaaaaa caccagccag ggtaaaccta gttttccgtg ttttgcctgc
    23881 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    23941 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    24001 atgccgtttc aagttcctgc cagttttgcg gatgatgata tttcagctcg aacatcgcgg
    24061 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    24121 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    24181 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc
    24241 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    24301 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    24361 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    24421 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    24481 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    24541 tttctgccgg atgcagctcc agccagtcgg caatattggc aattaagcga cgtccgcgat
    24601 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    24661 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct acttcccagt tgcaccacaa
    24721 tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    24781 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    24841 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    24901 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    24961 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    25021 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    25081 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    25141 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    25201 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    25261 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    25321 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    25381 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    25441 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    25501 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    25561 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    25621 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    25681 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    25741 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    25801 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    25861 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    25921 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    25981 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    26041 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    26101 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    26161 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    26221 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    26281 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    26341 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    26401 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    26461 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    26521 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc
    26581 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    26641 gggtcaaaag cattcagccc ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga
    26701 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    26761 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    26821 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    26881 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    26941 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    27001 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    27061 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    27121 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    27181 acatcatcca gcgctttttc tgcatgcgct ttcagctcaa catatttctg atcggcggga
    27241 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    27301 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    27361 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    27421 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    27481 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    27541 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    27601 acaatcaccc gacctataac gaccggctca agatcatcat cacttttcag aatcctcgca
    27661 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    27721 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    27781 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    27841 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    27901 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    27961 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    28021 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    28081 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    28141 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    28201 cgtgaatttg tggaaaccgc gctgcggatt agcggctcat ggaccgatta tccgctggaa
    28261 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    28321 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    28381 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    28441 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    28501 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    28561 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    28621 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    28681 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    28741 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    28801 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    28861 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    28921 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    28981 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    29041 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    29101 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    29161 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgggt aatgctgcca
    29221 acttactgat ttagtg
//
LOCUS       DADLCQ010000046        29048 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000046 DADLCQ010000000
VERSION     DADLCQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.046"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            108..1139
                     /gene="mdtN"
                     /locus_tag="JQG95_001844"
     CDS             108..1139
                     /gene="mdtN"
                     /locus_tag="JQG95_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY5888412.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWLVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1139..3187
                     /gene="mdtO"
                     /locus_tag="JQG95_001845"
     CDS             1139..3187
                     /gene="mdtO"
                     /locus_tag="JQG95_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY5888413.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYG
                     EPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIV
                     VGLYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALA
                     LQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLA
                     SEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPAA
                     KPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGS
                     SYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLAAWIATSSERSS
                     YIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTL
                     PQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDS
                     EERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLA
                     TALSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
                     "
     gene            3184..4650
                     /gene="mdtP"
                     /locus_tag="JQG95_001846"
     CDS             3184..4650
                     /gene="mdtP"
                     /locus_tag="JQG95_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY5888414.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4848..5087
                     /locus_tag="JQG95_001847"
     CDS             4848..5087
                     /locus_tag="JQG95_001847"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888415.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5097..5267
                     /locus_tag="JQG95_001848"
     CDS             5097..5267
                     /locus_tag="JQG95_001848"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY5888416.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5316..6308
                     /locus_tag="JQG95_001849"
                     /pseudo
     CDS             5316..6308
                     /locus_tag="JQG95_001849"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            6360..6995
                     /locus_tag="JQG95_001850"
                     /pseudo
     CDS             6360..6995
                     /locus_tag="JQG95_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
     gene            7089..7778
                     /locus_tag="JQG95_001851"
     CDS             7089..7778
                     /locus_tag="JQG95_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY5888417.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="JQG95_001852"
     CDS             complement(7856..9169)
                     /gene="gltP"
                     /locus_tag="JQG95_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY5888418.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="JQG95_001853"
     CDS             complement(9511..10107)
                     /gene="nrfG"
                     /locus_tag="JQG95_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY5888419.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="JQG95_001854"
     CDS             complement(10104..10487)
                     /gene="nrfF"
                     /locus_tag="JQG95_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY5888420.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10480..12198)
                     /locus_tag="JQG95_001855"
     CDS             complement(10480..12198)
                     /locus_tag="JQG95_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY5888421.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLCLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="JQG95_001856"
     CDS             complement(12218..13174)
                     /gene="nrfD"
                     /locus_tag="JQG95_001856"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY5888422.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="JQG95_001857"
     CDS             complement(13171..13842)
                     /gene="nrfC"
                     /locus_tag="JQG95_001857"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY5888423.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHVPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="JQG95_001858"
     CDS             complement(13839..14405)
                     /gene="nrfB"
                     /locus_tag="JQG95_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY5888424.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="JQG95_001859"
     CDS             complement(14450..15886)
                     /gene="nrfA"
                     /locus_tag="JQG95_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY5888425.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16279..18237
                     /gene="acs"
                     /locus_tag="JQG95_001860"
     CDS             16279..18237
                     /gene="acs"
                     /locus_tag="JQG95_001860"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY5888426.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAMHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            18437..18751
                     /locus_tag="JQG95_001861"
     CDS             18437..18751
                     /locus_tag="JQG95_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY5888427.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18748..20397
                     /gene="actP"
                     /locus_tag="JQG95_001862"
     CDS             18748..20397
                     /gene="actP"
                     /locus_tag="JQG95_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY5888428.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20575..21867
                     /locus_tag="JQG95_001863"
     CDS             20575..21867
                     /locus_tag="JQG95_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY5888429.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22021..23670)
                     /locus_tag="JQG95_001864"
     CDS             complement(22021..23670)
                     /locus_tag="JQG95_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY5888430.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23821..25170)
                     /locus_tag="JQG95_001865"
     CDS             complement(23821..25170)
                     /locus_tag="JQG95_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5888431.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="JQG95_001866"
     CDS             complement(25876..26340)
                     /gene="soxR"
                     /locus_tag="JQG95_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY5888432.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26426..26749
                     /gene="soxS"
                     /locus_tag="JQG95_001867"
     CDS             26426..26749
                     /gene="soxS"
                     /locus_tag="JQG95_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY5888433.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="JQG95_001868"
                     /pseudo
     CDS             complement(26752..28329)
                     /gene="pdeC"
                     /locus_tag="JQG95_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28758..29039
                     /locus_tag="JQG95_001869"
     CDS             28758..29039
                     /locus_tag="JQG95_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888434.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7288 a         7777 c         7349 g         6634 t
ORIGIN      
        1 gggcaaatat caatctggca tttgtcggaa ttatttatac cgcccttttt gctctctacg
       61 ccatgctgtt ctggctggca tttttctaat ctatttgaga cgcgctcatg gaaagtacgc
      121 cgaaaaaagc acctcgcagt aaattccctg ctctgttagt ggttgcgttg gcgctggttg
      181 cccttgtttt cgttatctgg ctcgtagaca gtgcgccatc aactaatgac gcttacgcgt
      241 cagcagatac cattgatgtg gtgccggaag tcagcggtcg cattgtagaa ctggcggtca
      301 ccgacaacca ggcggtgaaa cagggcgatt tgctgttccg catcgacccg cgcccgtacg
      361 aagccaacct ggcgaaagcc gaagcctccc tcgcggcgct ggataagcaa attatgctca
      421 cccagcgtag cgttgacgcc caacagtttg gtgccgactc ggttaatgcc acggtagaaa
      481 aagcccgtgc cgccgcgaaa caggccacag atacattacg ccgcaccgag ccattactga
      541 aagagggttt tgtctcagca gaagacgttg accgtgcaag aacggcgcag cgcgccgcag
      601 aagcggatct taatgccgta ttgttacagg cgcagtcagc cgccagcgcc gtcagcggcg
      661 tcgatgcgct ggtggcgcaa cgtgcggcgg tcgaagcgga tattgccctg accaaactac
      721 atctggaaat gacgaccgtt cgcgcgccat ttgatggccg gatcatttcc ctcaaaacct
      781 ccgtcgggca atttgcttct gccatgcgcc ctatttttac cctcatcgac actcgtcact
      841 ggtacgtgat cgccaacttc cgcgaaaccg atctgaaaaa tattcgctca ggtacacccg
      901 caacgattcg cctgatgagt gacagcggca aaaccttcga gggtaaagtg gattcgattg
      961 gctacggcgt gctaccggat gacggcggcc tggtgctggg cggcctgcca aaagtgtctc
     1021 gttctattaa ctgggtccgc gttgcccagc gttttccggt caaaatcatg gtcgataaac
     1081 ctgacccgga aatgttccgc atcggcgctt cggcagtcgc taatcttgag ccgcaataat
     1141 gagcgcgctc aactccctgc cattaccggt ggtcaggctg ctggcgttct ttcatgaaga
     1201 gttaagcgag cggcgacccg gccgcgtgcc gcagatcgtg caactctggg taggctgcct
     1261 gctggtgatt ctgatctcga tgacttttga gatccctttt gtggcgttat cgctggcagt
     1321 gctgttttac ggtatcggta ttcagtcgaa cgcgttttac accaaatttg tggcgatctt
     1381 gtttgtggtt gccacggtgc tggagatcgg cagcctgttt ttgatctaca aatggtcata
     1441 cggcgaaccg ttgatccgat tgatcatcgc cgggccgatc ctgatgggct gcatgttttt
     1501 gatgcgcacc catcggctgg gactggtctt tttcgctgtc gccatttacg ggcaaacctt
     1561 ccccgccatg ctcgactatc cggaagtagt cgtgcgctta acgctgtggt gtatcgttgt
     1621 tggcctctat ccaaccttgc tgatgacgtt aatcggcgtg ctgtggtttc ccaatcgtgc
     1681 cattacgcaa atgcatcaag cgcttaatga tcggcttgat gatgccatta gccacctgac
     1741 ggacagcctc gcaccgctac ccgaaacgcg gattgaaaga gaggcgctgg cgctgcaaaa
     1801 actgaatgtc ttttgtctcg cggacgatgc caactggcga actcaaagcg catggtggca
     1861 aagctgcgtg gcaacggtaa cctacattta ctcgacgctg aatcgctacg atcccacctc
     1921 ttttgctgat tctcaggcaa ttattgaatt tcggcaaaaa ttagcttcag aaatcaacaa
     1981 gctgcagcat gccgttgctg aaggtcagtg ctggcaaagc gactggcgga tcagtgaaag
     2041 tgaagcggtg gcggcacggg aatgtaacct ggagaatatc tgccagacgt tgttacaact
     2101 gggccagatg aacccgaata cgccgccaac gcccgccgcc aaaccgccat caatggtcgc
     2161 cgatgctttt accaatccag actatatacg ctacgcggta aaaacattgc tcgcctgttt
     2221 gatctgttac accttttaca gcggcgtgga ctgggaaggc attcacacct gtatgctgac
     2281 ctgcgtgatc gtcgctaatc caaatgtcgg ttcgtcgtac cagaagatgg tgctgcgttt
     2341 tggcggggcc ttttgcggcg cgattctggc gctgttattc acgctactgg tcatgccctg
     2401 gctggacaat attgtcgaat tgctgtttgt gctggcaccg attttcctgt tggccgcatg
     2461 gattgccacc agctctgaac gctcttctta tatcggcaca cagatggtgg tcaccttcgc
     2521 gctcgccacg ctcgaaaacg tttttggccc agtgtacgac ctggtggaaa ttcgcgatcg
     2581 cgccctgggt atcatcattg gtaccgtggt gtccgcgatg atttacacct ttgtctggcc
     2641 tgaaagtgaa gcgcgcacgc tgccgcaaaa actggctggc gcgctgggta tgttaagtaa
     2701 agtaatgcgg atcccacgcc agcaggaagt cacggctctg cgcacttatc tgcaaattcg
     2761 tatcggtctg catgcggcgt ttaatgcctg tgaagagatg tgccaacgcg tggcgctgga
     2821 gcgtcaactg gacagcgaag aacgcgcctt actgattgaa cgttcgcaaa cggttattcg
     2881 tcagggccgc gatatccttc acgcctggga tgccacctgg aattcggcgc aggcgctgga
     2941 taacgcacta cagccggaca gagcaggtca gtttgccgac gccctggaga aatacgctgc
     3001 cggtctggca accgcactca gccgttctcc tcaaataacg cttgaagaga cacccacctc
     3061 gcaggccatc ctgcccacct tattaaaaca ggagcaacac gtctgccagc ttttcgcccg
     3121 cttgccagac tggacagccc cggcattaac gcccgccacg gaacaggcac aaggagccac
     3181 gcaatgatca atcgtcaact ttcacgtctg ctgttgtgca gcattctcgg cagcacgacg
     3241 ctgatttccg gctgtgccct ggtacgtaag gattctgcgc ctcatcaaca gctcaaaccg
     3301 gaacaaatca aactggccga tgatattcat cttgccagct ccggctggcc gcaggcgcag
     3361 tggtggaaac aactcaatga cccgcagttg gatgccctga tccaacggac gctaagtggt
     3421 tcacacaccc tcgccgaagc gaaactgcgg gaagaaaaag cgcaatcgca ggccgatttg
     3481 ttagatgccg gttcacagtt gcaggtggca gcgttaggga tgcttaaccg ccaacgtgtc
     3541 tcggcgaacg gctttttaag cccttatgcg atggatgcgc ccgcactggg gatggacggg
     3601 ccgtactata cggaagccac agtaggtttg tttgccggac tggatcttga tttgtggggt
     3661 gtgcatcgct cagcggttgc cgccgccatt ggcgcgcata atgccgcgct ggcagaaacc
     3721 gcagcagtag agctatcgct gaccacgggc gtagcgcagc tttattacag tatgcaggcc
     3781 agctatcaga tgctcgatct gttagaacaa actcgcgatg tgattgatta cgcggtgaaa
     3841 gcgcaccaaa gtaaagtggc gcacggtctg gaagcacaag tgcctttcca cggcgcgcgg
     3901 gcacagattc tggcggtcga taaacaaatt gctgccgtca aagggcaaat caccgaaacg
     3961 agagaatctc tgcgcgcatt gattggcgcg ggagccagcg atatgccgga gatcaaacct
     4021 gtggcattac cacgagtcca gaccggcatt ccggcgacac tctcttatga gttgctcgcc
     4081 agacgcccgg atctgcaagc catgcgctgg tatgttcagg cgtcattaga tcaggtggat
     4141 tccgcgcggg cgttgttcta tccgagcttt gatatcaaag cgtttttcgg ccttgatgcc
     4201 atccacctgg ataccttatt caaaaaaacc agtcgccagt tcaacttcat cccgggtctg
     4261 aaattgccgc tgtttgacgg tggacggttg aatgccaatc tcgaaggcac gcgcgccgcc
     4321 agcaacatga tgattgaacg ttacaaccag tcagtactga acgcggtgcg tgacgttgcc
     4381 gtcaacggca cgcgtctgca aacgctcaac gacgagcgag aaatgcaggc tgaacacgtg
     4441 gaagcaacgc gcttcaccca gcgcgctgcc gaggccgcct atcagcgcgg cttaaccagc
     4501 cgcttacagg ccactgaagc ccggttgcca gtacttgccg aagagatgtc attactgatg
     4561 ctggacagcc gccgggtgat ccaaagcatt cagttgatga aatcgctggg cggcgggtat
     4621 caggcggctc ccgtcgtcga gaaaaaataa aatgtctgcc gcgtgatggc tgtcacgcgg
     4681 tatttcgttt cgtcacgtca aaactgacga cagcctgttt ttcgtcagag ttttgaataa
     4741 atagtgcccg taatatcagg gaatgacccc aaataaaatg tggcataaaa gatgcatact
     4801 gtagtcgaga gcgcgtatgc gtgatttgat taactggagc gagaccgatg aaaaaagtcg
     4861 tcacggtttg cccctattgc gcatcaggtt gcaaaatcaa cctggtcgtc gataacggca
     4921 aaatcgtccg ggcggaggca gcgcagggga aaaccaacca gggtaccctg tgtctgaagg
     4981 gttattatgg ctgggatttc attaacgata cccagatcct gaccccgcgc ctgaaaaccc
     5041 ccatgatccg tcgccagcgt ggcggcaaac tcgaacctgt ttcctgagat gaggcactga
     5101 attacgttgc cgagcgcctg agcgccatca aagagaagta cggtccggat gccatccaga
     5161 cgaccggctc ctcgcgtggt acgggtaacg aaaccaacta tgtaatgcaa aaatttgcgc
     5221 gcgccgttat tggtaccaat aacgttgact gctgcgctcg tgtctgacac ggcccatcgg
     5281 ttgcaggtct gcaccaatcg gtcggtaatg gcgcaatgag caatgctatt aacgaaattg
     5341 ataataccga tttagtgttc gttttcgggt acaacccggc ggattcccac ccaatcgtgg
     5401 cgaatcacgt aattaacgct aaacgtaacg gggcgaaaat tatcgtctgc gatccgcgca
     5461 aaattgaaac cgcgcgcatt gctgacatgc acattgcact gaaaaacggc tcgaacatcg
     5521 cgctgttgaa tgcgatgggc catgtcatta ttgaagaaaa tctgtacgac aaagcgttcg
     5581 tcgcttcccg tacagaaggc tttgaagagt atcgtaaaat cgttgaaggc tacacgccgg
     5641 agtcggttga agatatcacc ggtgtcagcg ccagtgagat tcgtcaggcg gcacggatgt
     5701 atgcccaggc gaaaagcgcc gccatcctgt ggggcatggg tgtaacccag ttctaccagg
     5761 gcgtggaaac cgtgcgttct ctgaccagcc tcgcgatgct gaccagtaac ctcggtaagc
     5821 cgcatgcggg tgtaaacccg gttcgtggtc agaacaacgt gcagggtgcc tgcgatatgg
     5881 gcgcgctgcc ggatacgtat ccgggatacc agtacgtgaa agatccggct aaccgcgaaa
     5941 aattcgccaa agcctggggc gtggaaagcc tgcctgctca taccggctat cgcatcagcg
     6001 agctgccgca ccgcgcagcg catggcgaag tgcgtgccgc gtacattatg ggcgaagatc
     6061 cgctgcaaac tgatgcggag ctgtcggcag tacgtaaagc ctttgaagat ctggaactgg
     6121 tcatcgttca ggacatcttt ataaccaaaa ccgcgtcggc ggcggatgtc attttgccgt
     6181 caacgtcgtg gggcgagcat gaaggcgtgt ttactgcggc tgaccgtggc ttccagcgtt
     6241 tcttcaaagc agttgaaccg aaatgggatc tgaaaacgga ctggcaaatc atcagtgaaa
     6301 tcgccacccg tatgggttat ccgatgcact acaacaacac ccaggagatc tgggatgagt
     6361 tgcgtcatct gtgcccggat ttctacggtg cgacttacga gaaaatgggc gaactgggct
     6421 tcattcagtg gccttgccgc gatacttcag atgccgatca ggggacttct tatctgttta
     6481 aagagaagtt tgataccccg aacggtctgg cgcagttctt cacctgcgac tgggtagcgc
     6541 caatcgacaa actcaccgac gagtacccga tggtactgtc aacggtgcgt gaagttggtc
     6601 actactcttg ccgttcgatg accggtaact gtgcggcact ggcggcgctg gctgatgaac
     6661 ctggctacgc acaaatcaat accgaagacg ccaaacgtct gggtattgaa gatgaggcat
     6721 tggtttgagt gcactcgcgt aaaggcaaaa ttatcacccg tgcgcaggtc agcgatcgtc
     6781 cgaacaaagg ggcgatttac atgacctacc agtggtggat tggtgcctgt aacgagctgg
     6841 ttaccgaaag cttaagcccg attacgaaaa cgccggaata caaatactgc gccgtgcgcg
     6901 tcgagccgat ctccgatcag cgcgccgccg agcagtacgt gattgacgag tacaacaagt
     6961 tgaaaactcg cctgcgcgaa gcggcactgg cgtaatacag tcctttctac agcctccttt
     7021 cggaggctgt ttttttatcc attcgaactc tttatactgg ttactcccta cccaatcgta
     7081 ttatcaaaat gaaaaaaatt atcgcattga tgttgttttt gacattcttt gcccacgcga
     7141 acgactccga gcctggcagc cagtatttaa aggcagcaga ggccggggac cgacgtgcac
     7201 aatattttct tgccgacagc tggtttagct ccggcgattt gagcaaagcc gaatattggg
     7261 cacagaaagc cgccgacagc ggtgatgctg atgcctgcgc gctgttggcg cagatcaaaa
     7321 tcaccaatcc ggtcagtctg gactatccac aagcaaaagt tcttgcagag aaagcggcgc
     7381 aagcgggcag taaagaaggt gaagtaacgc tggcgcatat tctggtaaat actcaagcgg
     7441 gtaaaccgga ttatccaaag gcgatttcgc tgttagaaaa cgcctcggaa gatctggaga
     7501 atgactccgc cgtcgatgcc caaatgctgc ttggtttgat ttacgccaac ggcgtgggca
     7561 ttaaggccga cgatgacaag gcaacctggt atttcaaacg cagctctgca atttcccgaa
     7621 ccggttattc cgagtactgg gcgggaatga tgttcttaaa cggtgaggaa ggttttatcg
     7681 agaagaacaa acaaaaggcg ctgcactggt tgaacctgag ctgtatggaa gggtttgata
     7741 ccgggtgtga agagtttgaa aaattaacca acggttaagg taggcctgat aagacgcggc
     7801 aagcgtcgca tcaggcatcc acacattgcc gggggatatc cccggcaatc ttcaattatt
     7861 gatccgcagt tttatcaaat ttgcccagca cttcacgctc ataagccagc gcctttttgc
     7921 ggtcaaattt gtgttcccac ttggcaatca ccagcaccgc cagcgcatta cccaccacgt
     7981 tcagcgcagt acgcgccatg tcgaggatac ggtcaacacc agcaataaac gccagacctt
     8041 ccagcggaat acctacgcta cccagcgttg ccagcaacac cacaaacgac acgccagcca
     8101 cgccagcaat ccctttcgag gtcaccatca gcgtcagcac cagaatgatt tcctgccaga
     8161 tggacaggtc aatgccatac agctgagcga tgaaaatagc ggcaatactt tgatacagcg
     8221 tcgaaccatc aaggttaaaa gagtaaccgg tcggcaccac gaagctggtg atcgacgccg
     8281 gtgctccgta ggcttccatc ttctcaataa ttcgcggcag tacgctttca gagctggcag
     8341 tggagtacgc cagaatcagc tcatctttca gaatacgaat caggatccag acgcttaacc
     8401 cgcacagacg tgccacaatt cccagcacta ccagcgcgaa gaacagaatg gcgaaatgca
     8461 ccagcagcac cagtttcgcc agtggccaca gagacgagaa accaaagtta gccaccgtca
     8521 ccgcaatcag cgcaaacaca cccaccggtg cataacgcat caccatgtga gtcactttaa
     8581 acatggtttc agagatggag cggaacacgg tcaccagcgg ttcacgatgc gtcgcgggca
     8641 gggaagaaag ccccagacca aacagcaccg agaaaaagat gatcggcagc atttcgcctt
     8701 tcgccatcga cgccacaatg ttcgtcggca ccagcgacaa aatcgtgccc ataatgccgt
     8761 gggaactgct ttgtaccgct tccgtagtgc tctgatattt cgagatatcg acggtcgcca
     8821 gctgcgacat atccaccccg gcaccgggct ggaagacgtt cgccagagtg atccccaaaa
     8881 tgatggcgac ggtggtgatc acctcgaagt aaataatggt tttcgcgcca atacgcccga
     8941 gctgtttggc atcaccaaca cccgcgatac ccaccaccag cgtggagatc acaatcggca
     9001 caacaatcat tttgatcaga tggatgaaga tatcacccgc cggagagagc aaattgacga
     9061 ccagccagtc gcggctgtcg ctatggtagt gcaggtagct tcccaggaga atgcccagca
     9121 ccatagcaaa cagaatctgc caggccaggc tgaattttat atttttcatc agaataactt
     9181 cctcaatgga gcgccgttcc ccgttcagaa acgcgagaac gtgacatact gccagggcat
     9241 gaattgtgtt gcgttggttt gtagattttt ttaacgctta tgatcagtat catttgcgcg
     9301 gtatgttgcg caacccttta acaacagagc ttttcgctgc attaatgcat aataacgcga
     9361 gtttatggca ggattaatga ataaccattt attatggaaa gatttttatt tgtttaatct
     9421 gcaataatac tgattggtga ttatttttgg catatgttca ttcgattatt ttttatgcag
     9481 ctcaatgaca taatcttcat caccagatga ttaatccgat ctccgctgca acagttttgc
     9541 catattaatc gactcgacca gctgtgtccg gttgatccgg ggtgagttga gatccatcac
     9601 tttttgccat agttcgatgg cttgcgcgta attcgcctgc ataaacgcat cggaagccag
     9661 cagcatcaga gcggtgattt cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg
     9721 agtctgggcg gtcatatgct ggctggcctg gtaataaagc accgtcgcca gcgccgcata
     9781 cagttcagcg ttctcaccac gtagttgcaa cgcctgacga tacgccagca gcgaattgct
     9841 gtaatcgttt tgccacagat agtactcgcc cagtaacgcc cattgttcgc tgttttgtgg
     9901 attagcgcgg attttatcct gcaatgcctg aagctgcgct tccggggttt gctggctggc
     9961 aaactgatgt agcggatcgc gctgacgctg atactccgca cgtaccgcct gccattttgg
    10021 gcttaacaga taactgccga cacagaggaa taccatcaat atcgtgagtg ttgtcagcat
    10081 tttcaccggt atttgaggct gtttcatcgc ttagccctca ctcgccagag gatcagtgcc
    10141 atcagcaata acaacaccac tggcagcgcc cacaacacca gcgtctgacc cgttaacggt
    10201 gggttatagc gaacaaaatc tccgtagcgt tcggtcatcc agccaatgat ttcgacttcg
    10261 ttcttcccct ccgacaccat gctataaacc tgatggcgca tactgaccgc caccggcgca
    10321 ttggattcga gtaagttttg attctggcac tgcggacaac gtaactggct ggcaatattt
    10381 aacgcctgtt gctgttgttg cggattggcg aattgccagg tgtctacaac ctgagcgcgc
    10441 gcaaaacagg taaataacag cagtagcgtg agaagccctt tattcatcgc gcttcttccc
    10501 ccgccatccg cttaacaatg cgcccgcaat catcaacaat cctccccccc agatccagcg
    10561 cacaccgctt tgtacataca aacgaaaagc gtaacgatcc gctctggttt tttcacccat
    10621 gaccgcatac cagtcatgga tgccgttcca gcgaattgac ggttccatca tttgctgacg
    10681 gcgtgcttca taaaaacgcc gctccggcgt taattcacca atgcgttgct gatggtcaaa
    10741 cagcgccact atcgcttttt cgctggtgta attgcctctg gcttgcagat cgagtcgctc
    10801 aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat
    10861 ctcctgacga ctgacactgg aaaccacgat ccccgcggca aataacagca cgccagcatg
    10921 agctaccagc gccggaagct gcacgcgttt gccagagaca aacgtcgcca gcacaatcac
    10981 caccagcatc aacagaccaa acggtaacgt cgcgcggtta aaatacggcg cgccaacgga
    11041 gaggcgtccc cagcccagca ggccgtaaat catcggataa agcgttccca ccagcacgat
    11101 cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt aacatttccc gcgataaccc
    11161 cgaaaaacgc accgccgggc caccgtcccg cgctcgccag ccatacagag ccaaagacgc
    11221 aaggctaatc agtgcaaaca ggctgaacaa cggcacggcg cggacgttat ccagtgcgaa
    11281 cgcatgaacc gaaaccagaa tgccagaacg gacaattaag gtgcccagca gcgacagcat
    11341 cagagtaact atcgccagca acagcgacca gtggcggaaa atcccccgct ggcgtgtcag
    11401 ggacaaactg tgcagcagcg cagtggcaga aagccagggt aataaagagg cgttttccac
    11461 cggatcccag aaccaccagc cgccccagcc gagttcgcag taggcccacc aggaaccgag
    11521 gatgatcccc gccgttaatg cactccagcc aggtaacgcc cagcgccagc aaattcgggc
    11581 gctggccgca tcaaactcgc cgcgcagtag actcgccagc gccacgctcg ccgctaccat
    11641 caaaccgcca tagccaaggc aaagcagcgg tggatgaaag ataagaccgg gatgttgcag
    11701 catcggattg agatcgcggc cttcgattgc tggtggaaat atccgcacga agggatcgga
    11761 ccacagcacc acaaacagta gcagtgcggc gagcatgaga gataaaacgg ctagcgtcag
    11821 cggaaatagc ggatcgcttt gctgccgata atgccaggca aacagcgcgc tccaggcgga
    11881 aagcagcagc acccaaagca gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt
    11941 gagttcccac gacagcaggc tgtagctatg ttgggcgaca taaatgaccg agaaatcgct
    12001 gatgagaaaa caatacgtca gtacgccaaa ggcgagcagc aggagcgcga actgcgccag
    12061 aatgccgatg caagtgagtc gcatcatggc aggccagcgc aaccgcactc ccgcaaaggc
    12121 cgtcaacggg gtcaacacgt tgaccccgag acttaacaac aacgccagaa aaccgacttc
    12181 aggaaggaat acgtccagaa accacctctt ttctggctta cgccaccgtc aactgtccgg
    12241 cgtagagaat gaaaaagcgc agcatcaaca cgccgaccag actggcccca cacgccgcca
    12301 gcacggcagg aatgccggaa ctgcgattga cccacggttt gagcaacatt ggcacaatca
    12361 gccccagccc ggcgacacca agccagaacc accacgtcca gaaaccgcca cctaatgccg
    12421 ccaccagcgc acgaacttta ccgtcatcgc ccagcgccag accgacaaaa aacgccacca
    12481 gcaggaagat ttcaccccat accacgggga tttccatccg gtgaacaaag tgcgcttccg
    12541 tggaatgcgg gttactgcgt tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg
    12601 acgagatgcc ggagaagagg agcagcaccg gcaggctcgg gttattgagg aacggatacg
    12661 atttcagcgc cgacagcaga aagccggtat aagcccccag cagcaccgcc aacaccagca
    12721 tcagcgtttc cagtccgcga tgaacgggcg tcagcaggct cagaaccttt tgcacgatcc
    12781 ccagcttcgg caaccagcgt tgttgcaggg caagcaaatc atgttcaaag attttcgcca
    12841 gccacagcac cagcaccacc atgtagagct gaaacagcat cacccccatc gacatcaccg
    12901 aggtaaaact gtagtggaac atcagcttcc agaaggtcca cggtcttgtc aggtggaaga
    12961 cgaggatcaa cagaccgagg atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca
    13021 acgtactgtc tgcaccgccc gcctgcgggt agaagcgacg taacagcacg gccagcgtca
    13081 ccagaccggc agaaatacca atcaaaaaca ggtagatggc aatcggccag tcccacacca
    13141 gcgattcaaa atgaaatgcg gaagtctgcg tcattggctc acctccccgt atttaaacgg
    13201 tacgcggtac agcttcggtt tggttcccag cgccagcttg tagcggtaag taggcttctg
    13261 gcgcagcagt tgcgaaatct cactgttagg atcgtccaga ttgccaaacg tcagcgcctt
    13321 cgtcgggcaa gcttcaacgc acgcgggaag cttaccggcc tgcaaattgg tcttacgaca
    13381 gaaatcgcat ttgtccgccg tcttcgtgac cggatggata aagcgcacgc ggtacggaca
    13441 ggcggcgatg cagtactgac aaccgacgca gagatcagga ttaacatcga caatcccgct
    13501 agcggcatca cgaaaagacg caccggtcgg gcagacgtca acgcacggca catgatcgca
    13561 gtgctggcaa gacttacgga agaagcgata tttcacatca ggaaattcgc cctgcggctc
    13621 gctacgaata atcgtcaggc gcgagacgcc ttccggcacc ttgttcactt cccgacaagc
    13681 atccatacag gcggtacagc cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac
    13741 cccattgata ttcaacgttt tcgccaccac gcgccctgcg gtgccgctga cggctgccag
    13801 cacgccgacg ccggtgagaa actggcgacg agaccaggtc atggctgctc cttaagcaac
    13861 ggaacagacg ccgggttaaa gttcggattg gtgcgctgat cgctgtggca atcgacgcaa
    13921 atcttaatcc gtcctttgtc acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa
    13981 tggcagctgg cgcacgccac tttggttacg tggacatcgt gcggccagaa cgctttttgc
    14041 aactgttccg gcagatgaca ggacatacag acgctgttct gctctccgac cttatacatc
    14101 ggctcgttaa agcgcatcac atctttcacc ccttcgcggt gttgcggtga tgcttggccg
    14161 tgacagttgg tgcaggtgac cggcaatttg ttattcgggt tgataacgga agcatgtttg
    14221 ccatgcatac cttcggtatc tggtttatga cagtccagac aggcggcatc cgggttacgc
    14281 tgctgggtaa cttcgtagcg atcaccagat gcctgcaccg caggggtagc ggtaattccg
    14341 ttcaggctcc acaacaggcc cgacgccagc accccagcag ttaataacga acgtaatacg
    14401 ctcatattca ctccattgtg ttttccgccc cttgcgaggc ggaacggggt tattggctta
    14461 acagaccgtt tttacgtgcc tgctcttccc actgcgggat caccgttttg atgaagtcct
    14521 gcttctcggc cttgatttgt tccatgttca ggccaatggc ctgctgggct ttctctttgg
    14581 tcgagatatc cgggatctgg atttcatggg tgataccttt ggtcgccagc aggcgcgcca
    14641 gtttggtgcg cgcatccgcc gctttatcca tcgccgtacc gagcatccgt agaccttctt
    14701 ccggtgcgtg catatgaata ccgtgggaag cgatcgccag atcccaacgc cactgggcat
    14761 gacggatatc gtcctgaatc ggcttcattt cggcttccgt cgcgcctgca tccagcgccg
    14821 ctttcgcttc gaagtgagcg tgaaccagtt gatcttcaac cttgattttc aggtcgttaa
    14881 tcgattgctt acgttccgcg accacttttt gcaaggcagc tttatcctgg gtatggcagt
    14941 tcgcacaagt ctgggcgaag ttatcaaacg gattaccaat tttatggtcg gtgtagagtt
    15001 tgccttcggc gttctgcact tttggcatat ggcagtcgat acaggtcacg ttgtttttac
    15061 cgtgaatgcc cgctgtccag gtttcatatt ccgggtgctg cgctttcagc attggcgttt
    15121 tcgacaggga gttagtccag tcagagaagg caattttgtc gtaatactgc tccatatttt
    15181 cgactttcat gccgtcatcc cacgggaatt taaccgcttt gtttttgccg tcgaagtaat
    15241 actccacatg gcactgaccg caaaccatcg attgctggtc gaaacgtccg gctttctcaa
    15301 aaggtttacc aatggcttcc atcgcgcgag ccgcatacgg acgggaaagg gttaactccg
    15361 gtttgccttt ggcgaactct ggtgaggcgg tgttatggca gtcggcacaa cctaagttgt
    15421 tgacgatttc cgggccgccg cgcgcccatt taccgtggaa gtagccatct tcgccgtctt
    15481 tctggatcag acgcgccaca tccgggcttt tacaactcca gcacgccatc ggtagcggac
    15541 catcttcagc gtttttcggc gcaccggtac gcagggtttc acgcacatcg gtcacagcaa
    15601 aggcatgtcc acgaggcttg ttgtaatcgc gcgagaaggg ataccccgcc cacaggatca
    15661 ccagccgtgg atcttccgcc agggcgtcaa cacgctctga ctgttccgag gtggctttcc
    15721 aggagagata ttgatcgtga tgctgcgggg caaaggtttc attcttcgct tccacagtta
    15781 caggttttgc gggagctgcc gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa
    15841 tcaataagct gaagatacgg cgtgcgttta tttttatcct tgtcataggg gcttcatccg
    15901 aattgcgcca ttgttgcaat ggcggttttt attgtttttc acgacagtaa ccgcacctac
    15961 actgtcatga cattgctcac ccctatgtgt aacaaataac cacactgtga atgttgtctt
    16021 taatcaattg taagtgcatg tgaaatacca ctttagagtt agtcagtatc ttcctctttt
    16081 tcaacagcat gcataactgc atgttcctca aagaattaat caacttttgt tggtgacctt
    16141 caaaaattac cctgccgttt atttgcacca ttctactttt gcgtgatctg tcgcccaaat
    16201 actaaacaaa actgccaata cccctacatt taacgcttat gccacatatt attaacatcc
    16261 tacaaggaga acaaaagcat gagccaaatt cacaaacaca ccattcctgc caacatcgca
    16321 gaccgttgcc tgataaaccc tcagcagtac gaggcgatgt atcaacaatc tattaacgta
    16381 cctgatacct tctggggcga acagggaaaa attctcgact ggatcaaacc gtaccagaag
    16441 gtgaaaaata cctcctttgc ccccggtaat gtgtccatta aatggtacga ggacggcacg
    16501 ctgaatctgg cggcaaactg ccttgaccgc catctgcaag aaaacggcga tcgcaccgcc
    16561 atcatctggg aaggcgacga cgccagccag agcaaacata tcagctataa agagctgcac
    16621 cgcgacgtct gccgcttcgc caataccctg ctcgagctgg gcattaaaaa aggtgatgtg
    16681 gtggcgattt atatgccgat ggtgccggaa gccgcggttg cgatgctggc ctgcgcccgc
    16741 attggcgcga tgcattcggt gattttcggc ggcttctcgc cggaagccgt tgccgggcgc
    16801 attattgatt ccaactcacg actggtgatc acttccgacg aaggcgtgcg cgccgggcgt
    16861 agtattccgc tgaagaaaaa cgttgatgac gcactaaaaa acccgaacgt caccagcgta
    16921 gagcatgtgg tggtactgaa gcgtactggc ggaaacatcg actggcagga agggcgcgac
    16981 ctgtggtggc acgacctggt tgagcaagcc agcgatcagc accaggcgga agagatgaac
    17041 gccgaagatc cgctgtttat tctctatacc tccggttcta ccggaaaacc aaaaggcgta
    17101 ctgcacacta ccggcggtta tctggtgtac gcggcgctga cctttaaata tgtctttgat
    17161 tatcatccgg gtgatatcta ctggtgcacc gccgatgtgg gctgggttac cggacacagt
    17221 tatttgctgt acggcccgct ggcctgcggc gcgaccacgc tgatgtttga aggcgtaccg
    17281 aactggccga cgcctgcccg tatggcgcag gtggtggaca agcatcaggt caatattctc
    17341 tataccgcac ccacggcgat ccgcgcgctg atggcggaag gcgataaagc gattgaaggc
    17401 accgaccgtt cgtcgctacg cattctcggt tccgtgggcg agccaattaa cccggaagcg
    17461 tgggagtggt actggaaaaa aatcggcaac gagaaatgtc cggtggtcga tacctggtgg
    17521 cagaccgaaa ccggcggttt catgatcacc ccgctgcctg gcgctaccga gctgaaagcc
    17581 ggttcggcaa cacgtccgtt cttcggcgtg caaccggcgc tggtcgataa cgaaggtaac
    17641 ccgctggaag gggctaccga aggtagcctg gtgatcaccg actcctggcc tggtcaggcg
    17701 cgtacgctgt ttggcgatca cgaacgtttt gagcagacct atttttccac cttcaaaaat
    17761 atgtatttca gcggcgacgg cgcgcgtcgc gatgaagatg gctattactg gatcaccggg
    17821 cgtgtggacg acgtgctgaa cgtctccggt caccgtctgg ggacggcaga gatagagtcg
    17881 gcgctggtgg cgcatccgaa gattgccgaa gccgccgtag taggtattcc gcacaatatt
    17941 aaaggtcagg cgatctacgc ctatgtcacg cttaatcacg gggaggaacc gtcaccagaa
    18001 ctgtacgcag aagtccgcaa ctgggtgcgt aaagagattg gcccgctggc gacgccagac
    18061 gtgctgcact ggaccgactc cctgcctaaa acccgctccg gcaaaattat gcgccgtatt
    18121 ctgcgcaaaa ttgcggcggg cgataccagc aacctgggcg atacctcgac gcttgccgat
    18181 cctggcgtgg tcgagaagct gcttgaagag aagcaggcta tcgcgatgcc atcgtaaccc
    18241 acaattgccg gatgcgaatc ggtaacggtt tgtaggcctg ataagacgcg acagcgtcgc
    18301 atcaggcatt gattgccgga tgcggcgtaa acgccttatc cggcctacat tcggcaaggg
    18361 ttacccgagc gttaaccttc tcccataagg gagcgggaat taaaacaatc cctacattac
    18421 ctctggagaa tctgtgatga atggtacgat ttatcagcgg atagaagaca atgcgcattt
    18481 cagggagtta gtcgaaaaac ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc
    18541 agtttatatc ggctttattt tactgatcgc cttcgcgccc ggctggctgg gcacgccgct
    18601 gaatccgaac accagcgtca cacgcggtat tccaattggt gttggagtga ttgtgatctc
    18661 ctttgttctc accggtatct acatctggcg ggcgaacggc gaattcgacc gtcttaataa
    18721 tgaagtcctg catgaggtac aagcatcatg aaaagagttc tgacggcgct tgccgccaca
    18781 ctccctttcg cagctaacgc cgcggatgct attagcgggg ccgtagagcg ccagccaacg
    18841 aactggcagg cgattattat gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac
    18901 tgggcatcaa aacgcgtacg ttctcgtagc gactactaca ccgcaggcgg caatatcact
    18961 ggcttccaga acgggctggc gattgccggg gactatatgt ccgccgcctc attcttgggg
    19021 atctccgcgc tggtgtttac ctccggctat gacgggctga tctactcgtt gggcttcctg
    19081 gtgggctggc caatcattct gtttctgatt gccgaacgtc tgcgtaacct gggacgctac
    19141 acctctgccg atgtggcctc ttatcgtctg aaacaagggc cgattcgtat tctttcggcc
    19201 tgtggttctc tggtagtggt ggcgctttac cttatcgctc agatggtagg cgcaggtaaa
    19261 ctgatcgagc tgctgtttgg ccttaactat cacattgcgg tggtgctggt cggcgtgctg
    19321 atgatgatgt acgtcctgtt cggcggcatg ctggcgacca cctgggtaca aattatcaaa
    19381 gctgtgctgt tgctgttcgg tgccagcttt atggccttta tggtgatgaa acacgtcggc
    19441 tttagcttca acaatctgtt cagcgaagcg atggcggtac acccgaaagg tgtcgacatc
    19501 atgaaaccgg gcggactggt gaaagatccg atctccgcgc tctctctggg gctgggactg
    19561 atgtttggta cggcgggctt gccgcacatt ctgatgcgct tctttacagt cagcgatgcc
    19621 cgcgaagcac gtaagagcgt gttctacgcc accggattta tgggctactt ctatattctg
    19681 acctttatta tcggcttcgg cgcgatcatg cttgttggtg cgaatccgga atataaagac
    19741 gcggcgggcc atctgattgg tggtaacaac atggcggccg ttcacctggc gaatgcagtg
    19801 ggcggcaacc tattcctcgg ttttatttca gcggttgctt tcgccactat cctcgcggtg
    19861 gttgcggatc tgacactggc gggcgcatcg gcggtttcgc atgacttgta cgctaacgtc
    19921 ttcaaaaaag gcgcgaccga acgtgaagag ctgcgggtat caaaaatcac cgtactgatc
    19981 ctcggcgtga ttgcgattat cctcggcgtg ctgtttgaga atcagaacat cgcctttatg
    20041 gtaggccttg cgtttgccat cgcggcgagc tgtaacttcc cgatcattct gctttccatg
    20101 tactggtcga aactgactac ccgtggcgcg atgctgggcg gctggctggg gctgattacc
    20161 gcagtggtgc tgatgatcct cggcccgact atttgggtac agatcctcgg tcacgaaaaa
    20221 gccatcttcc cgtatgaata cccggcgctg ttctctatca gcgtggcatt cctcggcatc
    20281 tggttcttct cggcaaccga taactcagcg gaaggggcgc gcgagcgtga actgttccgc
    20341 gcgcagttta tccgctccca gaccggcttt ggcgttgagc aaggccgcgc acattaatct
    20401 ctccccttcc ccggtcgcct gaccggggaa tacgcttcct ctccaacatg catcaccttt
    20461 tcccataata ttaaacaaat aaactcatta aaaaatgagc gatttttgac agtcgtagaa
    20521 aatgataatg cagagaatat gccttttctt tcttgttaat tataaggata ttttatgcgc
    20581 tataatggtt taaataatat gtttttccct ctttgccaga ttaacgataa ccactctatc
    20641 acaagttcat cacatacaaa gaaaacaaaa tcagataatt acagcaaaca tcataaaaac
    20701 acgttaattg acaataaagc cctctctctt ttcaaaatag atgatcatga aaaagtgata
    20761 ggcttgattc agaaaatgaa aagaatttat gatagtttac catcaggaaa aatcacgaaa
    20821 gaaacggaca ggaaaataca taaatatttt atagatatag cgttatatgc aaataataaa
    20881 tgtgacgata gaattacgag aagagtttac cttagtaaag aaaaggatgt atccattaag
    20941 gtggtatatt ttataaataa tgtcgccgtc cataataata ctatcgaaat tccacagata
    21001 gtaaatggtg gttacgattt ttcacacctt agcctgaagg gtatcgtgat taaagatgaa
    21061 gatttatcca attcgaattt tgcaggttgc agactacaaa acgctatttt ccaggactgt
    21121 aatatgtata aaacgaattt ttattacgcc ataatggaaa aaatactttt tgataattgt
    21181 attctcgatg actcaaattt cgctcagata aaaatggccg acggaactct aaatgcatgc
    21241 tccgctatgc atgttcaatt ctacaatgca gcaatgaata gagccaatat taaaaatacc
    21301 ttccttgact attcaaattt ttatatggcg tacatggctg aggtaaatct ttataaagta
    21361 atagcgccat atgttaattt atttaaagcc gaccttagtt tctctaaact cgatttaatt
    21421 aactttgaac atgctgatct gtctcgcgtc aatctgaaca aagcaatcct ccagaatata
    21481 aacttaattg atagcaaact cttttgtacg tggctgacaa atacattcct cgaaatggtt
    21541 atatgtaccg gctctaatat ggctaatgtt aattttaata atgccaattt aagcaactgc
    21601 catttcaact gttctatttt aacaaaagcc tgtatgttta atacccgtct ctatcgggtt
    21661 aatttcgatg aggctagcgt ccagggaatg ggcatttcca ttctccgtgg ggaggaaaat
    21721 atccccattg atagtgatac cctggtaaca ctacagaaat tctttgaaga agattgtacc
    21781 tctcatactg gcatgtcaca aactgaggat aatattaatg cagtcgctat gaagattact
    21841 gcagatatta tgcaacacgc agattgaggt taagtcgaag aacagccaat gaattgactt
    21901 tacaagagag ataacttatt tgttttagaa tcatgaatgt tcgcccgagc taacctgtta
    21961 aaggtgcaaa agattgcacc tttttcagat tacctcgggc ctggttacgt cgctttcggc
    22021 ttactgattt tcctcaatta gcaacgcttc aagcaaatcg agatcgtgca gtaatttttg
    22081 cagcgtttcg ttgctgatct cccgcgtggc gcgcaggtgg tacagttcag cacgttcaga
    22141 acgcaatgcc gccagacgga agcgacgctc aaggttctct tcctgcacgg aactttcaac
    22201 gtcgttacgt ccatcggcgc gacgacgcag gttaccaatg acgcgagaac tgacctccgt
    22261 aagcaactgg ttatcgatat tctcttcggt atccgccgcc agacgctcct ccattttctg
    22321 gatcgccaca atcgccactt ctgccgttgc cgctcgcgca atacgttctt ctttcaattg
    22381 ttgcgaatgg tccgcgactt caatgtgttg tagcagaata ggcaacatca ccacgccgac
    22441 aaacagcgag aagagaatga cgccagccgc caggaacacc agctcatagc gcgccgggaa
    22501 gacgttacca tccggcaaga gcagcgggat tgagagcaca ccggccagag tgatcgcccc
    22561 acgcacccca gcgaaagacg cgatcaggat ttctcgtgtg gtccacgaac caaactccat
    22621 cggcttcttc ttcaggaagc ggttgctgaa ctttttcatc gtccacagcc agccgaaacg
    22681 gaccagcatc agcgccgcat atatcagaat aatatcggta aacagcatcc agatttcgac
    22741 gttagggtcg atttctgccg ccatcagcga cgtctccaga atacccggca gttgcagacc
    22801 taacagcagg aacaccatgc cgttaaagac aaattccagc atcgcccagg tgctgtttgc
    22861 gcgcaggcgc attgccagtg gcgcacggcg catcacaccg gagcgggtga tggtcatccc
    22921 ggcggcaacc gcagcgagga tgcccgaaac gccaatatgt tcggcaatca gataagaagc
    22981 gaatggcagc aagaacagca gaacgatctg cgtcgcgggt tcatcaccgc cccagcggct
    23041 gaggaatcgc agcgagcgac catacagcca gctcaccaca aaaccggcaa gaataccgcc
    23101 aatggctact ttcataaatt cgaccgtcgc accgccgaca gtgaagatca tcgtccccat
    23161 cgccactgcc acggcaaact tcaacgacac caggccggaa gcgtcattca tcaacgcttc
    23221 gccctgcaaa atgcccatga tttttttcgg gatgcgccct tcgccaacaa tcccggagag
    23281 cgccacagca tcggtcggag aaagtaccgc cgccagcgca aaggcgggga tcagcggaat
    23341 gcccggcacc acccagtaaa taaggaagcc aatgccgacc acggtgacca ccaccagcgc
    23401 cagcgcgagg ccgaaaatct ctcgaccatg ttcgagaaat tcacgggtcg gcgttttcca
    23461 gccatcagcg aacagcaacg gcgggataaa caagactaaa aagagttcag gatcaaactc
    23521 cacatgcaaa ccaaacgtcg gccacgccag tagcgcaccg atggcgattt gcataagcgg
    23581 aagcgggatc tgaaagggca tgacacgagt gaccaccccg gacagcgaga ccacgagggt
    23641 cattatcagt atggtgaaga agatttccat gcgttccctg tttgcgatgt tttttatgat
    23701 ctgagaaaga cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
    23761 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
    23821 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
    23881 gatgttcaac ttgcgccatt caccggaaac cagacgacca atcaccagag tcgcgaagcc
    23941 gatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
    24001 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
    24061 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag aaagcggaga
    24121 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaacc cggttttacc
    24181 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagctg cacccaccag
    24241 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccgccgt cgatgatctg
    24301 cccatcttta tccagcaggt tcgcctggcc ggcgacagcg cggatagttc cggtggcatc
    24361 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
    24421 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
    24481 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
    24541 aatggtcagc agaatgccac cagggacttt cagtttttcc aagccgatga tcaccgccag
    24601 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
    24661 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaggaacag
    24721 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
    24781 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
    24841 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa acgccgtcag
    24901 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
    24961 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
    25021 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
    25081 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
    25141 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
    25201 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
    25261 gcggcaattt ggtgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
    25321 gtaaaaaaaa gcaaacgatt atccggcgat catagcggct ttttcaaccg aagtttgctg
    25381 cttttttctc ttttattgtc ttgacgatga aaaaccaggc aattgatgcg caaacgttat
    25441 cgtcgatgtg caagattgca gcgttatcga gccgtatacc gttctggatt gcgataaaaa
    25501 atcgtctgag atgacaaaaa tattgcggct aaaaagtata cttttacact ggcgaagaaa
    25561 cgtcggtgac atacaattaa agcatcaaca ccaaccggaa cctccaccac gtgctcgaat
    25621 gaggtgtgtt gacgtcgggg gaaaccctcc tgtgttccag cgggatagag agaaagacaa
    25681 agaccggaaa acaaactaaa gcgcccttgt ggcgctttag ttttgtgttt gcggataatt
    25741 ccaaaagagc tcgatcagtc ccctcgcccc ttcggggaga gggttagggt taggggaaca
    25801 ggcagcacca gtgcaaatat ttaccctcac cccggcccca tagttaacga tgagtcagct
    25861 tgtccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
    25921 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
    25981 aatacatccg tccagttcgt cacgcagcgc cactaaggta tgaatgcgcc gatccaactc
    26041 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttctgg
    26101 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcgatttt
    26161 gataattgcg acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
    26221 gatactagta atcaaccctt tgctttcata gaaatgcagc gccgataccg ccacaccgct
    26281 gcgtttcgcc acttcgccag gggttagcag cgctttaatg cggggtaatt tcttttccat
    26341 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
    26401 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
    26461 atggattgac gtgcatattg accagccgct taacattgat gtcgtcgcaa aaaaatcagg
    26521 ctattcaaag tggtacttgc aacggatgtt ccgcacggtg acgcatcaga cgcttggcga
    26581 ttacattcgc caacgccgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
    26641 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
    26701 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
    26761 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
    26821 cgccttcgca aaaaaccatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
    26881 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
    26941 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
    27001 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
    27061 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaattaatcg
    27121 gcgtcatttt ttcgacatca agaaatgcat gctcagtcac ttcaaattta atttgctgcg
    27181 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
    27241 aatcggaggc cgacaggtta atagaaacat agcgatcggc atgtgttgcc aggtacgcgc
    27301 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
    27361 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaggccaac
    27421 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
    27481 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
    27541 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
    27601 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
    27661 tggctgcaat tgggcgtttt gtcgaataaa ttgtcgcata taaatagcca tttctttgta
    27721 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
    27781 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
    27841 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
    27901 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
    27961 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
    28021 agcataaaaa atggttatca cgaattcatt aatataaaga tagccacgaa taatattcaa
    28081 cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag ctgcatcgct
    28141 cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt gagcaaaggt
    28201 tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata gcgaaagaat
    28261 gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt gtcgtgcacg
    28321 atgactcatt gctatcaatt tcctgcctgt ttatacccag ccattgcaag gtgaaaccct
    28381 ggctaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa
    28441 ttgatacctt tttaatcgtc acctggatga aaataattca ttgatattca aaattaattt
    28501 tctacaggaa tacagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata
    28561 atttgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata
    28621 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
    28681 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
    28741 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
    28801 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
    28861 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
    28921 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
    28981 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
    29041 acgtagaa
//
LOCUS       DADLCQ010000047        28431 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000047 DADLCQ010000000
VERSION     DADLCQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.047"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            564..1334
                     /locus_tag="JQG95_001870"
     CDS             564..1334
                     /locus_tag="JQG95_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY5888435.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JQG95_001871"
     CDS             1325..1798
                     /locus_tag="JQG95_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY5888436.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPRLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JQG95_001872"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JQG95_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY5888437.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JQG95_001873"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JQG95_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY5888438.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JQG95_001874"
     CDS             3233..3727
                     /locus_tag="JQG95_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY5888439.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JQG95_001875"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JQG95_001875"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY5888440.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JQG95_001876"
                     /pseudo
     CDS             complement(6410..7432)
                     /locus_tag="JQG95_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
     gene            7571..8485
                     /locus_tag="JQG95_001877"
     CDS             7571..8485
                     /locus_tag="JQG95_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5888441.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8700..10061
                     /locus_tag="JQG95_001878"
     CDS             8700..10061
                     /locus_tag="JQG95_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888442.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JQG95_001879"
     CDS             complement(10110..11774)
                     /gene="tsr"
                     /locus_tag="JQG95_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY5888443.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JQG95_001880"
     CDS             complement(11893..12339)
                     /gene="hpaR"
                     /locus_tag="JQG95_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY5888444.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12611..13900
                     /locus_tag="JQG95_001881"
     CDS             12611..13900
                     /locus_tag="JQG95_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY5888445.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPAPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13897..15363
                     /gene="hpaE"
                     /locus_tag="JQG95_001882"
     CDS             13897..15363
                     /gene="hpaE"
                     /locus_tag="JQG95_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY5888446.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVSEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15365..16216
                     /gene="hpaD"
                     /locus_tag="JQG95_001883"
     CDS             15365..16216
                     /gene="hpaD"
                     /locus_tag="JQG95_001883"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY5888447.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16226..16606
                     /locus_tag="JQG95_001884"
     CDS             16226..16606
                     /locus_tag="JQG95_001884"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY5888448.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16674..17477
                     /gene="hpaH"
                     /locus_tag="JQG95_001885"
     CDS             16674..17477
                     /gene="hpaH"
                     /locus_tag="JQG95_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY5888449.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALMDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17488..18291
                     /gene="hpaI"
                     /locus_tag="JQG95_001886"
     CDS             17488..18291
                     /gene="hpaI"
                     /locus_tag="JQG95_001886"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY5888450.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPASIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18313..19668
                     /gene="hpaX"
                     /locus_tag="JQG95_001887"
     CDS             18313..19668
                     /gene="hpaX"
                     /locus_tag="JQG95_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY5888451.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19678..20568
                     /gene="hpaA"
                     /locus_tag="JQG95_001888"
     CDS             19678..20568
                     /gene="hpaA"
                     /locus_tag="JQG95_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY5888452.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTQAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDEKYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCPPTQFRGR"
     gene            20676..21532
                     /locus_tag="JQG95_001889"
                     /pseudo
     CDS             20676..21532
                     /locus_tag="JQG95_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21759..23317
                     /gene="hpaB"
                     /locus_tag="JQG95_001890"
                     /pseudo
     CDS             21759..23317
                     /gene="hpaB"
                     /locus_tag="JQG95_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23335..23847
                     /locus_tag="JQG95_001891"
     CDS             23335..23847
                     /locus_tag="JQG95_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY5888453.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24240..26390
                     /gene="btsT"
                     /locus_tag="JQG95_001892"
     CDS             24240..26390
                     /gene="btsT"
                     /locus_tag="JQG95_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY5888454.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26440..26643
                     /locus_tag="JQG95_001893"
     CDS             26440..26643
                     /locus_tag="JQG95_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY5888455.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26654..27610
                     /gene="yjiA"
                     /locus_tag="JQG95_001894"
     CDS             26654..27610
                     /gene="yjiA"
                     /locus_tag="JQG95_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY5888456.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27679..>28431)
                     /locus_tag="JQG95_001895"
     CDS             complement(27679..>28431)
                     /locus_tag="JQG95_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY5888457.1"
                     /translation="TKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTED
                     FNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQHA
                     DNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQAL
                     VNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYL
                     TKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6959 a         7432 c         7127 g         6913 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctagattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgaccaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcact cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgccca ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc taacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cctaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tatccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc
     8581 gactaaaaat gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt
     8641 caaccttgcg taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg
     8701 tggaaaaaga aaatatcacc ctcgatccgc gttcttcatt tactccatct tcgtcggcag
     8761 atattcccgt gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa
     8821 ccaccgccat gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc
     8881 tgtcggtagc aaatttaacg attcgtgaag aattgggatt aagtgccacc gaaatcggcg
     8941 ctttgctctc cgtgttttca ctcgcttacg gcattgcgca acttccttgc ggcccactat
     9001 tggatcgtaa aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc
     9061 aggccatgtc tggcatggtg cacagcttta cgcagttcgt gttggtgcgt atcggtatgg
     9121 ggattggtga agcgccgatg aacccatgcg gtgtaaaagt cattaacgac tggttcaaca
     9181 tcaaagagcg cggacgcccg atgggcttct tcaacgcagc ttctaccatt ggcgttgctg
     9241 taagcccacc gattctggcg gcaatgatgc tggtgatggg ctggcgcggg atgtttatta
     9301 ccattggtgt actggggatt tttctcgcca tcggctggta tatgctctat cgcaaccgcg
     9361 agcacgtaga actgactgcc gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc
     9421 gccgagatcc gctcaatttt gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg
     9481 gaatgatgct cggattcagt ggcatcaact acactgcgtg gctgtatctg gcctggcttc
     9541 ctggttacct gcaaacagcc tataacctgg atttaaaaag cacagggttg atggcggcta
     9601 tccctttcct gtttggggct gccgggatgc tggtcaacgg ttacgttact gactggctgg
     9661 tcaaaggggg aatggctccg attaaaagcc gtaagatctg cattattacc gggatgttct
     9721 gttctgccgc ctttacgctg atagtaccac aagcgacaac atccatgatg gcggttctgc
     9781 tgattggcat ggcactgttc tgtattcact ttgccggaac atcctgctgg ggcttgatcc
     9841 acgtcgcagt tgcttctcgc atgactgcgt cggtgggcag tatccagaac tttgccagct
     9901 tcatctgcgc ctctttcgcg ccgatcatta ctggttttat tgttgataca acccactcat
     9961 tccgtctggc actaatcatt tgcggttgcg tcaccgcagc gggagcactg gcgtacatct
    10021 tcctggttcg ccagccgatc aacgacccgc gtaaagatta aactccctat gcccgataac
    10081 attttgctta tcgggcattt ttatggcgat taaaacgttt cccagttctc cccgctatct
    10141 gccactgcca ttttacgcgg cgtagctggc gtcacggttt ttaccacagc cgatgtttca
    10201 cgctgctgtt gctgctgttg ctgaatccgg aacactgcca cagcttcggt caggcgactg
    10261 gcctgctctt ccagcgcggc ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt
    10321 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10381 tcatcagaag cagaagctat ttcgcccata atgtccgtca cgcgggtcac agcactgaca
    10441 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10501 accgagtctt caatcaggct tttaatttca cgaaccgcct gggcgctacg ctgggccaga
    10561 ttacgcactt ctcccgcaac caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct
    10621 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg taatgccgtc aattacgctg
    10681 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10741 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaatgccag atggctggcc
    10801 tggcgcgcat tctcggcgtt ctgtttcacc gttgcggtca gttgctccat gctggctgcc
    10861 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcatt attgccggta
    10921 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10981 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    11041 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    11101 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    11161 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    11221 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    11281 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11341 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11401 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11461 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11521 gaaccgctac caatattatt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11581 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tgggttgctg ctggcgaatg
    11641 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaaaaa cagaccgcct
    11701 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11761 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11821 caaaaacttt atgaaaaggt gtaacccgcc atccatggaa aaaccaggcg ctggttatca
    11881 gatactaaaa agttattcat tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc
    11941 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    12001 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    12061 tttggtcagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    12121 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    12181 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    12241 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12301 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12361 aaaatcatta atatagaaac aattaattat cacgttaccg ggactgagct gcccccgcaa
    12421 ttcttcattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12481 atctttaaat gtaaatagtt tgttaattag atcacattta tatcacttac ttttgccaaa
    12541 ctacgcaatc gggaataaaa gtaatcatta acatattaat gattaagatg atcgaatccg
    12601 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12661 tgcctggcag gaagcgttcc agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg
    12721 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12781 tgaaaaagta ctgagcggtg cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt
    12841 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12901 gccggaagag agcttttacc gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12961 cattggcgaa accgtggctc tcagcaatgt cgataatctg actatctata ccgagatcaa
    13021 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cacagctgct
    13081 gagcgccctg agcgaatttg ccacgctgaa tccaggcgat gctattctgc tcggcacgcc
    13141 acaggcgcgc gtggaaatac agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc
    13201 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac
    13261 gctgccacac ccgcacggta cgctgtttgc cctcggcctg aactacgccg accacgccag
    13321 cgaactggaa tttaagccac cggaagagcc gctggtgttc ctgaaagcgc cgaataccct
    13381 cactggcgac aaccagacct ccgtgcgccc gaacaatatt gaatacatgc actacgaagc
    13441 ggagctggtg gtagttattg gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga
    13501 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgcgactatc tggaaaacta
    13561 ctaccgccct aacctgcggg tcaaaagccg cgacggactg acgccgatgc tttcaaccat
    13621 cgtgccgaaa gaggcgatcc cggccccgca taatctgacc cttcgcacct tcgtcaacgg
    13681 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13741 ctacttaagc gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa
    13801 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13861 cctggtgaac cgaattgtga gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc
    13921 aacggcaaaa atgttgcagg taacgactac ttccagacta ccaatccggc aacgggtgaa
    13981 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    14041 aaagaggcgt tcccgaaatg ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    14101 cgtctgggcg atctgatcga ccagaacgtg tctgagatcg ccgcgatgga aaccgcggac
    14161 acgggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt
    14221 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    14281 atgctcaact acacgctggt gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac
    14341 gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14401 gtgctgaaga tgtcggaact ctccccgctg accgccgacc gcctgggtga gctggcgctg
    14461 gaagctggta ttccggcggg cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc
    14521 gatgcgctgg tccgtcatca tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc
    14581 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggagct gggcggtaaa
    14641 tcaccggtgc tgatttttga agatgccgat attgagcgtg cgctggacgc cgccctgttc
    14701 accatcttct caatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcag
    14761 agcatctatc cagaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14821 gatccgaacg atccgaatac ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa
    14881 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg
    14941 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaacggtg
    15001 ctggcggacg tagataaccg tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc
    15061 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac
    15121 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    15181 attgaagcag gcatggtgtt cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt
    15241 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15301 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15361 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcaa tgtatctctc
    15421 tgaactacca ggaaaaaacc acggttgtcg ccagggtgcg atcgacgggc ataaagagat
    15481 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata ctcactggct
    15541 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15601 cgagctgccg cattttattc gcgacatgac ctacaactac gagggcaacc cggagttggg
    15661 gcagctaatt gccgaggaag ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc
    15721 cagcctgaaa ctggaatacg gcacgctggt accgatgcgc tacatgaatg aagacaagca
    15781 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15841 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15901 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15961 ctacacccgc gagttcgacc gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg
    16021 ccagttcaaa gagttttgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    16081 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    16141 ggtggagttt attaccgagc tgttcccaag ctctggcacc ggtcaggtta acgctgtttt
    16201 cccgctgccc gcgtaaggac gttttatgcc gcactttatc gtggaatgta ctgaaaatat
    16261 tcgtgaagaa gcccgcttac ctgagctgtt tgccagtgtt aacactgcac tggcggcaac
    16321 ggggattttt ccgcttgggg gaatacgtag ccgggcgcac tggatcgata cctggcaaat
    16381 ggctgatggc aagcacgatt acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg
    16441 cagtctggaa agccgtcagg aagtgggcga aatgctgttt gatctgatta aaacgcactt
    16501 cgcctcactg atggagagcc gttacctagc gctttcattt gagattgcag agctgcaccc
    16561 gacgttgaat ttcaaacaga acaacgtaca cgcgttgttt aaataaattt gcaccagctt
    16621 ccaggccgga tgataaggcg ctgccgcatc cggcaatgcc acaggatatc gctatgttcg
    16681 ataaacacac ccacaccctg atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac
    16741 agatccgcgc gatctcgctg gattacccgg agatcaccat cgaagacgcc tacgccgtgc
    16801 agcgcgaatg ggttcgcctg aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg
    16861 gtctgacctc gaaagcgatg caggcaagct cgcagatcag cgaaccggat tacggtgcac
    16921 tgatggacga catgttcttc cacgatggca gcgatatccc gaccgatcgc tttatcgtgc
    16981 cgcgcattga agtggagctg gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca
    17041 cgctgttcga cgtttacaac gccacggact atgtgatccc ggcgctggag ctgatcgacg
    17101 ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg caaagtattc gacaccattt
    17161 ccgataacgc cgccaacgcc ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac
    17221 tggatctgcg ttggatctcc gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg
    17281 tcgccgctgg tgtgctgaat catccggcaa acggcgtggc ctggctggcg aacaaactcg
    17341 caccgtatga cgtacaactg gaagccgggc aaatcattct cggtggttcg ttcacccgcc
    17401 cggttccggc gcgtaagggc gacaccttcc acgtcgatta cggcaacatg ggctccatta
    17461 gctgccgctt tgtttaagga gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca
    17521 ggccgtccgc agattggatt atggctgggg ctgagcagca gctacagcgc ggagttactg
    17581 gccggaccag gattcgactg gttattgatc gacggtgagc acgcgccgaa taacgtgcaa
    17641 accgtgctca cccaactaca ggcgattgcg ccctacccca gccagccagt ggtacgccca
    17701 tcgtggaacg atccggtgca aatcaaacaa ctgctggacg taggcacaca aaccttactg
    17761 gtgccgatgg tacaaaacgc cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc
    17821 cccgccagta ttcgcggtgt gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt
    17881 cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt
    17941 gaggcaatga agaacttacc gcagattctg gacgtggaag gggtcgacgg cgtgtttatc
    18001 ggcccggcgg atctgagcgc cgatatgggt tatgccggta atccgcagca cccggaagta
    18061 caggccgcca ttgagcaggc gatcgtgcag atccgcgaag cgggcaaagc gccggggatc
    18121 ctgatcgcca atgagcaact ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc
    18181 gtcggcgttg acaccaccct gctcgcccgc gccgccgaag cgctggcagc acggtttggt
    18241 aatagttctt ctatttcgat ggcgaagcaa aacaacaact ctgtctacta aacgtacgat
    18301 gtggagcgca tcatgacgac ctctaccctg caatataaag ataataaaac tgttgaaatt
    18361 gaacatcgtg tcattaataa attatttcgt cgcctaatca tttttctttt tattctgttt
    18421 gttttttcgt ttcttgatcg catcaatata ggatttgccg gactgacaat gggcaaggat
    18481 cttggcctga cgtccacaat gtttggcctc gccgcaacac tcttttatgt cacgtacgtc
    18541 atgtgcggga tccccagtaa tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct
    18601 gggatcatgg tggtctgggg aattgcttcc acctgcacaa tgtttgcaac aagccccgaa
    18661 acactctatc tactgcgtat gctggttggt attgctgagg cgggtttttt acctggtatt
    18721 ttggtttatc tgacctggtg gttcccggca tatcatcggg ccagagccaa tgccttattt
    18781 atgatagcga tgccagtcac tatgatgctg ggattgatcc tttcaggcta cattctggcc
    18841 ttggatggac tgtggaatct aaaaggctgg caatggctct ttttgctgga aggtctgcca
    18901 tcggtcattc ttggtgtcgt cacctggttt tatctcaacg acacaccaga ccaggcgacc
    18961 tggcttgatg aaaacgaaaa acaggcttta aaaacgatga ttctccgtga acaagagagt
    19021 gcatttgccc atgcatctgt tccacgctca accttacgtg aagtcctgac cccagccatc
    19081 atgctatata cacttgctta tttttgcctg actaatactc tgagtgcgat taatatctgg
    19141 acgccgcaaa tcctacagag tttcaatact ggcagtagca acatcatgat tggtttgctg
    19201 gcggccatcc cccaattctg cacaattttt ggcatgatct ggtggagtcg tcgttccgac
    19261 cgtcttaaag aacgcaaaaa gcatacgatt cttccctacc tgtttgccgc tgccggatgg
    19321 ttactcgctt ctgcgacaga tcacagcctc attcaactga gcagcatcat tatggcctca
    19381 atgggatcat ttaccgcgat gactatcttt tggacaacac cggatcaagt tataagcttg
    19441 caatcgcgtg ccgttgcgat ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc
    19501 agtccattac ttatcggtat tttgaaagat gcaaccggca gttttagctc aggtttgtgg
    19561 tttgtagcag gattgctggt actgggcgcg ttagtcatga cccgaatccc tatggggagt
    19621 cgggaacact ctctttcatc tgaacttatc gcaccaaaac cgcaataagg agtcatgatg
    19681 tccagtgaag ctatctttga acatggttat gaaaatattg atttggtccg ggaatatgat
    19741 gcccgctacg ctggtgaaga tgtgcattat gaaacatttg cccggttagc gaatttcttt
    19801 ggacgcgata tgcgccctca ctggcacgat agatattttc aactccatta tctggtcact
    19861 ggacgcatta ctttacaact ggatgagcac ttctattctg tgcgcgcacc gctttttgta
    19921 ctaacaccac catcggtgcc acatactttc tttactgatc ctgatagcga tgggcatgtt
    19981 ctgaccgtac gtcaggagtt aatttggccc ctggtagaaa agctctggcc tggtagtgga
    20041 gatgccgcaa tgctacaagg tatctgtctg tcactggagg gaatggaaca tacacaggcg
    20101 gcattaaatc attattggcc gataatatcc ctggagtttc aacaaaataa gcaaggacgg
    20161 gaaattctat tgcaatcact ggcgcaaagt atttttacaa tcctactgcg tgaagcacca
    20221 ccaaatgata tttcaacatg cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat
    20281 cggctgattg acgaaaaata tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc
    20341 ggtatgagtg aatcacggct taccgagctg tgccgacgtt ttgccaatca atcgccgaag
    20401 agattgattt tcgaacgagt gagccgggaa gccagacggc ttctgctcta tagcgagcaa
    20461 agcatcaacc agatagccct tgatcttggc tacaaagatc ccgcctattt cgcccgtttt
    20521 ttcaaccgaa tgatgggttg cccaccaaca caattccgtg gtcgctgacc atttatcaac
    20581 ctgagagact cgacatgaaa atatctttac gctcactatt tgtggcgtgc ctgattgcgc
    20641 cagtggcatt cacctacgcc tccgaaccgc aataaatgaa aattgtacta cttggaacga
    20701 aaggtggccc ttctttatta aaagctgacc gtttaccgat ggcaacggca cttattattg
    20761 gcaataaaac ctagttaatt gatgctggtt acggggccag cttacaactg gtaaaaaata
    20821 atattccact gcgccagatt gataatattt tactcaccca tttacacagt gatcatattc
    20881 tggactatcc atcagtctta atgaatgcat gggctagcgg tctgcaggac aaaactattc
    20941 atgtattcgg tccgccaggc acccgagcca tgaccgctgc cagttggcag gtcttcgatc
    21001 gggacatcac actgcgtatg gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag
    21061 ctcaagatgt tggtgaaggc gtagtttata aagatgatac cgtgaccgtg actgcattaa
    21121 aggtccctca ttctcctttc cctgacggtg aggcttttgc ttatcgcttc gacacacaag
    21181 gcaagcgaat tgtcttctca ggagatacct cgtactttcc cccactggca actttctcag
    21241 aaggcgctga tatacttgtg catgaggccg tacatgagcc ctccgtcgct aagctggcag
    21301 acagtattgg caatggtaaa acactggcaa aagccattgc ttctcaccat acgactattg
    21361 aggatgtagg caaaattgcc aatgaagcaa aagtgaaaaa actcatcctt agccacctgg
    21421 taccaatcac cgttccggat gaaacatgga agcaagaagc acagaaaact attcaggccc
    21481 tatcgtcgtt ggtcatgaaa atatgacgat taccattcca ttcgccccat aaaaaattcc
    21541 gtccccgtta gcccttgtta gcggggaata ttatgatttg ccttactgta agttgatcac
    21601 gatcgcagtt tactatttaa ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa
    21661 cgtctcttcc cgacaaccgc gttcaacgct tcaattaaat aacaaaaaca acacacaaaa
    21721 ttaacaatcg tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg
    21781 ccagtaccca acgtcctttc accggggaag agtatctgaa aagcctgcag gatggtcgcg
    21841 agatctatat ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg
    21901 cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg caggactctc
    21961 tgtgttggaa caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga
    22021 aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgaatggtca cgcctgagct
    22081 atggctggat gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga
    22141 atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg
    22201 aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgtcatt tgccgaccga
    22261 taaagtgaaa gacgtttaca tcaagctgga aaaagagact gacgccggga ttatcgtcag
    22321 cggtgcgaaa gtggttgcca ccaactcggc gctgactcac tacaacatga ttggcttcgg
    22381 ctcggcacaa gtgatgggcg aaaacccgga cttcgcactg atgttcgttg cgccaatgga
    22441 tgccgatggc gtgaaattaa tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg
    22501 ctcgccatac gactacccgc tctccagccg cttcgatgag aacgatgcga ttctggtgat
    22561 ggataacgta ctgatcccat gggaaaacgt gctgatctac cgcgattttg atcgctgccg
    22621 tcgctggacg atggaaggcg gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct
    22681 ggcagtgaaa ctcgacttca ttacggcact gctgaaaaaa tcactcgaat gtaccggcac
    22741 cctggagttc cgtggtgtgc aggccgatct cggtgaagtg gtggcgtggc gcaacacctt
    22801 ctgggcattg agtgactcga tgtgttcaga agcgacgccg tgggtcaacg gggcttattt
    22861 accggatcat gccgcactgc aaacctatcg cgtactggca ccaatggcct acgcgaagat
    22921 caaaaacatt atagaacgca acgttaccag tggcctgatc tacctccctt ccagtgcccg
    22981 tgacctgaac aatccgcaga tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg
    23041 tatggatcac gtccagcgca tcaagatcct caaactgatg tgggacgcca ttggcagcga
    23101 atttggtggt cgtcacgagc tgtatgaaat caactactcc ggtagccagg atgagattcg
    23161 cctgcaatgt ctgcgccagg cacaaagctc cggcaatatg gacaagatga tggcgatggt
    23221 tgatcgctgc ctgtcggaat acgaccagaa cggctggact gtgccgcacc tgcacaacaa
    23281 cgacgatatc aacatgctgg ataagctgct gaaataacgc agcaggaggt taagatgcaa
    23341 ttagatgaac aacgcctgcg ctttcatgac gcgatggcca gcctgtcggc agcggtaaat
    23401 attatcacca ccgagggcga cgccggacaa tgcgggatta cggcaacggc tgtctgctcg
    23461 gtcacggata caccaccgtc gctgatggtg tgcattaacg ccaacagtgc gatgaacccg
    23521 gtttttcagg gcaacggcaa gttgtgcgtc aacgtcctca accatgagca ggaactgatg
    23581 gcgcgccact tcgcgggcat gacaggcatg gcgatggaag agcgttttag cctctcatgc
    23641 tggcaaaaag gtccgctggc gcagccggtg ctaaaaggtt cgctggccag tcttgaagga
    23701 gagatccgcg atgtgcaggc gattggcaca catctggtgt atctggtgga gattaaaaac
    23761 atcatcctca gtgcagaagg tcacggactt atttacttta aacgccgttt ccatccggtg
    23821 atgctggaaa tggaagctgc gatttaagct ccagcccctt cacgcattcg tcgaaggggc
    23881 atttttattg tgatctcaat cacatatcta cccgatgaat aattcttgcc caaccgctca
    23941 ccgccattcc tgaccgctta catccctaaa ataaccactc agttatttac cttactttac
    24001 gcgcacgtat ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca
    24061 acgcaaacct agaacggctt cggcctatga ttaacaaatt actctatgga ttttggtttg
    24121 caggaaggca gcaagtgagt gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa
    24181 cgaacgcagc tgccgcacct gtaagccaaa agacgacgag taaaatgcca ggttttacta
    24241 tggatacgaa aaaactattc aagcacatac cctgggtgat tctcggaatc atcggtgcat
    24301 tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg
    24361 tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta cagtctgtac atcgcccaga
    24421 aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt tattaacaac gacggtctga
    24481 actacgttcc gaccaaccgt tacgtgttgt ttggtcacca cttcgccgct atcgccggtg
    24541 ctggtccgct ggtgggaccg gttctcgccg cgcagatggg ctacctgcct ggcacgctgt
    24601 ggctgctggc gggggtagtg ctggccggtg cggtacagga ctttatggtg ctgtttatct
    24661 cctctcgccg taacggcgca tctctcggtg agatgatcaa agaagagatg gggccagtac
    24721 cggggactat cgcgctgttt ggctgtttct taatcatgat catcatcctc gccgttctgg
    24781 cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa
    24841 ccgtaccgat tgcgctgttt atgggtatct acatgcgctt tatccgtccg gggcgtgtgg
    24901 gtgaagtctc tgttattggt atcgtgctgc tggttgcctc tatctacttc ggtggcgtga
    24961 ttgctcacga tccgtactgg ggtccggcac tgacctttaa agacaccacc attaccttcg
    25021 cgctgattgg ctatgcgttt gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc
    25081 gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg
    25141 tggtgctgaa cccggaactg aaaatgcctg cgatgaccca gtacattgac ggtactggcc
    25201 cgctgtggaa aggcgctctg ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat
    25261 ctggcttcca cgcgctgatc tcttccggta cgacgccgaa actgctggct aacgaaaccg
    25321 acgcgcgttt cattggctac ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc
    25381 tggttgcagc gtccatcatc gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg
    25441 gccttggcat caccatgcct aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca
    25501 tggcgcagct gaaagacgtt accgcacacg cggcagcgac cgtcagctcc tggggcttcg
    25561 tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga
    25621 accgtgcagg tggtgcgccg acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc
    25681 tgccgatggc tgacatgggc ttctggtatc acttcggtat tctgttcgaa gccctgttca
    25741 tcctgaccgc gctggatgcg ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg
    25801 gtaacttcat cccgttcctg aaaaaaaccg actctctggt tgccggtatc atcggtactg
    25861 cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg
    25921 gcgttaagag cctgtggccg ctgttcggta tctccaacca gatgctggca gccgtagcgc
    25981 tggtactggg caccgttgtg ctgattaaga tgaagcgcac ccaatacatc tgggtaactg
    26041 ttgttccggc tgtatggctg cttatctgca ccacctgggc gctgggtctg aaactgttca
    26101 gcaccaaccc gcagatggaa ggcttcttct acatggcaag ccagtacaaa gagaagattg
    26161 ctaacggtac tgacctgacg gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca
    26221 actacaccaa cgcaggcctg agtattctgt tcctgattgt ggtgtacagc atcatcttct
    26281 acggtttcaa aacctggctt gcggtgcgta acagcgacaa acgtactgac aaagaaacac
    26341 cgtacgttcc aatcccggaa ggcggcgtga agatctcttc gcaccactaa ccgtgtttag
    26401 ccccgcttcg gcggggcttt gttcttatca gagtgaacta tgtttggtaa cttaggacag
    26461 gcaaaaaaat atctcggcca ggcggcgaag atgttgattg gtattccaga ctacgacaac
    26521 tacgtcgagc atatgaagac caaccatccc gacaagccgt acatgagcta tgaagaattc
    26581 ttccgcgaac gccagaacgc gcgctacggc ggcgatggta aaggcggtat gcgctgttgt
    26641 taatggagag agcatgaacc cgattgcagt taccctactc accggttttt taggcgcggg
    26701 caaaactacc ctgctgcgcc atattcttaa cgaacaacac ggctacaaga ttgccgtgat
    26761 tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg attggcgacc gcgccacgca
    26821 gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc
    26881 actactcgac ctgctggata atctcgacaa gggcaatatt cagttcgatc gtctggtgat
    26941 tgaatgcacc ggcatggccg atcccggccc gattattcag acctttttct cccatgagat
    27001 tttatgccag ctctacctgc tggacggcgt gattgcgctg gtggatgcgg tacatgccga
    27061 tgagcagatg aaccagttca ccatcgccca gtcccaggtg ggctacgccg accgtattct
    27121 gctgactaaa accgacgtcg caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat
    27181 caacgcccgc gcaccggtct acaccgtcac ccacggcgac atcgacctgg gcctgctgtt
    27241 caacaccaac ggttttatgc tggaagagaa cgtcgtcagc accaaaccgc gtttccactt
    27301 tatcgcggat aaacaaaacg atatttcgtc aattgtggtg gaactggatt acccggtaga
    27361 tatcagcgaa gtttcccgcg tgatggaaaa cctgctgctg gaatcggcgg ataaactgct
    27421 gcgttacaaa gggatgctgt ggattgacgg cgaacctaac cgcctgctgt tccagggcgt
    27481 ccagcgcctc tacagcgccg actgggacag gccgtggggc gatgaaaaac cgcatagcac
    27541 aatggtgttt atcgggattc agttgccgga ggaagagatt cgggcggcgt ttgcgggatt
    27601 gaggaagtaa gttcgggaaa gatcaacttt ttgagagttc caccgcaaca ttattacgag
    27661 tggaactctt tagatatgtc agaccaacca aagcttcttc aattgcgttt tcattttttc
    27721 atgtctcatt ttcagttcag ccaatcccca ttctgaagaa ttacaaacat cttttgttaa
    27781 ataaaaagag cttttactat taagtttctc ataaatagat ttctttttga taaaatcata
    27841 gtgttgtgtt tcagaatttt tggaaccact tatgagtgtt aaattaccta ttttatgcag
    27901 ccagtagaca tgctcttgag gttggaatct attaatccag tattcattaa ctaatgcttg
    27961 aggaagaata tgctcaattg tgattctccc cgtataggtt ttaatcacgc tctcatcttg
    28021 ttgctccata tctaagcgaa gcaaaattgc ttttattaag ttcacttgat tcggacgagg
    28081 ctcatataaa tcttcatcca gagcagctat aaaacctgaa ttatctgcat gttggtttat
    28141 ctgatttatc acagaatcaa aaggcatatc attatttatt gctaccagag cagaataaca
    28201 aaccatttct ctttggcttt taatttgttt ttttaaccac ccatgcatat ataccttctc
    28261 aaaggcggta ataaattgag aaaaatcatc taaattaaaa tcttctgtac gagccattcg
    28321 attcatgaat gccataaccg gaggaatcca ctcatctacc cctaaattac ttagcgatgc
    28381 tatttttcgt cgtattgaag ggtgttcaaa atcattctct aaaatcttag t
//
LOCUS       DADLCQ010000048        28338 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000048 DADLCQ010000000
VERSION     DADLCQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.048"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQG95_001896"
                     /pseudo
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JQG95_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(998..2020)
                     /locus_tag="JQG95_001897"
     CDS             complement(998..2020)
                     /locus_tag="JQG95_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5888458.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JQG95_001898"
     CDS             complement(2034..3536)
                     /locus_tag="JQG95_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY5888459.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQG95_001899"
                     /pseudo
     CDS             complement(3676..4632)
                     /gene="ytfQ"
                     /locus_tag="JQG95_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4942..5472
                     /gene="ppa"
                     /locus_tag="JQG95_001900"
     CDS             4942..5472
                     /gene="ppa"
                     /locus_tag="JQG95_001900"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY5888460.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="JQG95_001901"
     CDS             complement(5851..6192)
                     /locus_tag="JQG95_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY5888461.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JQG95_001902"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JQG95_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY5888462.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGNADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JQG95_001903"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JQG95_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY5888463.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12548
                     /gene="msrA"
                     /locus_tag="JQG95_001904"
     CDS             11910..12548
                     /gene="msrA"
                     /locus_tag="JQG95_001904"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY5888464.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12871..14214
                     /locus_tag="JQG95_001905"
     CDS             12871..14214
                     /locus_tag="JQG95_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY5888465.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14276..14482)
                     /locus_tag="JQG95_001906"
     CDS             complement(14276..14482)
                     /locus_tag="JQG95_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY5888466.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14807..15361
                     /locus_tag="JQG95_001907"
     CDS             14807..15361
                     /locus_tag="JQG95_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY5888467.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15424..16362)
                     /locus_tag="JQG95_001908"
     CDS             complement(15424..16362)
                     /locus_tag="JQG95_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888468.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="JQG95_001909"
     CDS             complement(16574..17314)
                     /gene="cysQ"
                     /locus_tag="JQG95_001909"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY5888469.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17504..19447
                     /gene="cpdB"
                     /locus_tag="JQG95_001910"
     CDS             17504..19447
                     /gene="cpdB"
                     /locus_tag="JQG95_001910"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY5888470.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19573..19953)
                     /locus_tag="JQG95_001911"
     CDS             complement(19573..19953)
                     /locus_tag="JQG95_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY5888471.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20042..20902
                     /gene="qorB"
                     /locus_tag="JQG95_001912"
     CDS             20042..20902
                     /gene="qorB"
                     /locus_tag="JQG95_001912"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY5888472.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21011..21976
                     /locus_tag="JQG95_001913"
     CDS             21011..21976
                     /locus_tag="JQG95_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5888473.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATVQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22084..22746
                     /gene="ytfE"
                     /locus_tag="JQG95_001914"
     CDS             22084..22746
                     /gene="ytfE"
                     /locus_tag="JQG95_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY5888474.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="JQG95_001915"
                     /gene_synonym="dagA"
     CDS             complement(22791..24203)
                     /gene="cycA"
                     /locus_tag="JQG95_001915"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY5888475.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24511..25131)
                     /locus_tag="JQG95_001916"
     CDS             complement(24511..25131)
                     /locus_tag="JQG95_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5888476.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25350..25895
                     /locus_tag="JQG95_001917"
     CDS             25350..25895
                     /locus_tag="JQG95_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888477.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25879..26526)
                     /locus_tag="JQG95_001918"
     CDS             complement(25879..26526)
                     /locus_tag="JQG95_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5888478.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26738..27529
                     /locus_tag="JQG95_001919"
     CDS             26738..27529
                     /locus_tag="JQG95_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY5888479.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27539..>28338
                     /locus_tag="JQG95_001920"
     CDS             27539..>28338
                     /locus_tag="JQG95_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY5888480.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6953 a         7542 c         6989 g         6854 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccact aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacaat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcga atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcgaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccatttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgctgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct tacggtcaat
     4141 ggcgacgctg gccccaacgg tgccctgcag ctccaccacg ttgcatggtt tgccattcac
     4201 ttcttttacc agccagtcac cgatcaactt accttcgagg atgttgtcgg cagtaacggt
     4261 ggtcatatag agagatttgt ctttcacatc gatggaacgg tcgagcaaga agaccgggat
     4321 ttcggcatct ttcgcctctt ttaataccgg ttcccaaccc gtcgcaacca ctggagcgat
     4381 aaagatcgca tccacccctt atgcaacgaa ggaacgtacc gctttaatct ggttttcctg
     4441 cttttgctga ccatcggcaa ttttcaacgt aattccgcgt ttttcggcct cacttttcgc
     4501 cacattggtt tctgcggcgc gccagccgga ttccgatccg acctgcgaaa atcctacggt
     4561 taatggagcg gccaacgcca tagacgacat ggctgccgag actgcagaga ctacaagtaa
     4621 gcgtttccac atagggttgt cctcgtcggg gtaattatta ttggtgaaaa gatgttcgcg
     4681 aaaaaactat agacaattcg ttatgtaacg gattgcgtta catcacactt caaaaaagta
     4741 aataaaacgt taatcacaag tttgtaatcg ctttcatctc actatgaaaa atgcggctac
     4801 ggttatggat tttcctgctc tgtataccgt cttaaaactg gcgaaaaagg aaaatgaaga
     4861 cgaaaacaag caaagacatt cggcgcgagt tggctataat acttggcact tgtttgccac
     4921 atatttttaa aggaaacaga catgagctta ctcaacgtcc ctgcgggtaa agatctgccg
     4981 gaagacatct acgttgttat tgagatcccg gctaacgcag atccgatcaa atacgaaatc
     5041 gacaaagaga gcggcgcact gttcgttgac cgcttcatgt ccaccgcgat gttctatccg
     5101 tgcaactacg gttacatcaa ccacaccctg tctctggacg gtgacccggt tgacgtactg
     5161 gtcccgactc cgtacccgct gcagcctggt tctgtgatcc gttgccgtcc ggttgccgtt
     5221 ctgaaaatga ccgacgaagc cggtgaagat gcgaaactgg ttgcggttcc gcacagcaag
     5281 ctgagcaaag aatacgatca cattaaagac gtaaacgatc tgcctgaact gctgaaagcg
     5341 caaatcgctc acttcttcga gcactacaaa gacctcgaaa aaggcaagtg ggtgaaagtt
     5401 gaaggttggg aaaacgcaga agccgctaaa gctgaaatcg ttgcttcctt cgagcgcgca
     5461 aagaataaat aagttcttct agcgcaataa ccctgaacgc cgggcttcgg ttagtaaggg
     5521 tttttttatg cccgcgataa ataaactctc tattccacca tcattattct cagcggttgc
     5581 aaggcttgaa cggtaagaac aagcaaaccc gaccaccatt ttgctgttca tagccacttg
     5641 ctggaagtta gccgacctca ctcatactca ccgttcaaag cgtgcccccg ttttacccct
     5701 gcacatttgt gaacgccagc agagtttttt atgcgattgc agatatggaa agcggctcgg
     5761 agagaaaagc aaaagatgga aaagttacaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggaggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcattac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgacccccag atgcagatta cccgctttta ccgccacgcc tagctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccgacgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgtgct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtctccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccaa cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttgaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg
    12541 aagcatagcg ttacgggtac aaatgtagtt tgttgataaa gtgctctttg tctatgcctg
    12601 atgcggctta aacgacttac caggcctaca aaatcgccca aattcaatat attgcaagga
    12661 ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg catttatcag cagtctgaat
    12721 tttaaccctc tggcgacttt acagtacctt acgctatact agccactgaa aatgccggtt
    12781 cactttcttc gaatcggctt tcaatgtgta tttcacacaa attaatcaac tttcccttcc
    12841 gagggtctgg cctgaaaggt cggataagat atgttaaaca gtattttagt catactctgc
    12901 ttgatcgctg taagtgcgtt cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa
    12961 atcaaactta aactgctggc tgatgaaggc aatataaatg cccaacgcgt tctgaatatg
    13021 caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg gtctgaacgc agtggcgatt
    13081 ctcggcggta tcgtcggtga tgcggcattt tctccagctt ttcacagcct gttctcccgc
    13141 tatatgtcgg cagagctctc tgagcaactg agctttattc tctctttctc gttagtgact
    13201 ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa
    13261 gcggtggctt tgcgtatcat caacccgatg cgcttctgcc tgtacgtttg caccccgctg
    13321 gtgtggttct tcaacggcct ggcgaacatg atcttccgta ttttcaaact gccaatggta
    13381 cgtaaagatg acatcacttc tgatgacatc tacgcggtag tggaagccgg tgcgctggcg
    13441 ggcgtgttac gtaaacagga acacgagctg attgaaaacg tctttgagct ggaatcccgt
    13501 accgttccgt cttcaatgac accgcgtgaa aacgtgattt ggtttgatct ccacgaagat
    13561 gagcaaagcc tgaagaataa ggtggcggaa catccgcact ctaagttcct cgtctgtaat
    13621 gaagatattg accacatcat cggttatgtc gattctaaag acctgctgaa ccgcgtgctg
    13681 gctaaccaaa gcctggcact gaacagcggc gtacaaattc gcaacacgct gattgtgccg
    13741 gatacgttaa ccctttcaga ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg
    13801 gtgatcatga acgagtacgc gctggtggtg gggatcatca ccctcaacga cgtgatgacc
    13861 acgctgatgg gcgatctggt cggtcagggg ctggaagagc agattgtcgc ccgtgatgag
    13921 aactcatggc tgattgacgg cggcacccca attgacgacg tcatgcgcgt gctggatatt
    13981 gacgagttcc cgcagtcggg caactacgaa accatcggtg gctttatgat gtttatgctg
    14041 cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg gctacaaatt tgaagtggtg
    14101 gatatcgata actaccgcat cgaccagctg ctggtgaccc ggatcgacag caaggccacc
    14161 gccctttcgc caaaactgcc tgacgctaaa gataaagaag aaagcgtcgc gtaaccccca
    14221 gaaacatcaa cggctcctga atcaggagcc gtcttattac tgcatagcac tttggttaag
    14281 ccatctctgt ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca
    14341 gcttatcctt cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaatgt
    14401 ccttgatgta taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt
    14461 tgtaccgttg gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca
    14521 caatcggccc aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg
    14581 gcaatgtttc caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc
    14641 tttttaccga agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg
    14701 accgcaaact ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc
    14761 agacccaagt ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa
    14821 gattctggca ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaat
    14881 cggtcagcga gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga
    14941 tcagtttagc tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca
    15001 acatattgct ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa
    15061 atcagcgaag ttaccgcacg atcgttacca gaccaccacc attgttaaca ccgacgacgc
    15121 gattccgggt tccggcatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc
    15181 ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga
    15241 agaaagttcc gctgtagtgg tgctggataa agacggtcgc gtgcaatggg ccaaagacgg
    15301 ggcgctcaca ccggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata
    15361 atggcaataa cataaattct ggaaaaataa ttttccccta tcgttatcta tgggataggg
    15421 gaattaagtc tctgagttct tacaccgaac taatactggt aacgctagtt gctctttttt
    15481 aaacgcctca atggtcgatt tatatttctc gcaaatcttt tctttggtaa tattactatc
    15541 tgcggccaga ttcgcactta ctatcgcatc cacttgttcg gctaattcga cttgttggca
    15601 acctgcatta accagaaaat tataaataag agagtaatct tcatcctcca aaacaacaga
    15661 ggaaccatta cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc
    15721 tatgactatg gcaaaattat catcaattat tttttcgtaa atataccatc catcgcattc
    15781 tttaattcca ccgcctggta tttggagttc aaatttaccc cgttttaatt tgcttactac
    15841 atcctccaat aaggcataac cgaaagatac gtcaccaaac gcctggcgaa tataaccttt
    15901 aatccttttt ggtatgtcag actgtttttt ctgcaaaata tcacaggcat acaatcttct
    15961 gttttgttct aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat
    16021 tgttatggga atttcttcgt attcaaaccc actgcctcgc aatgttattg tatattcagc
    16081 accgttatct ttaaggctga aactgataag aaaattatcc atggcattaa cagtttcatt
    16141 ttttgatgaa agcacttgat aactattttt atcaatctgt gttgcgcaac gaaaggactc
    16201 ccgcaaagtt aacagactat cataattcga aacccaatct tttttcttaa atgcatgctg
    16261 aatattatta aaaaaatcgt ttgaatgtgt ttttgtatgc gatgacttta caaataactc
    16321 caccgcacca ataaaattat tgtgtctgat cctgggtaac atatggctac cttattttat
    16381 ttggttattt tatgtcacca ttaaatatct cattatagaa gtaaactatc aaaaaacgct
    16441 cattattaaa atgtaactta ctagcaattg aatttttgca ctttaaatac aatctctttt
    16501 tatttataac tttttaggtt gggataaatc aggatggcag aatcaggaaa tacactgttt
    16561 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
    16621 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
    16681 cagcatgtcc ggcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
    16741 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    16801 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    16861 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
    16921 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    16981 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    17041 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    17101 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
    17161 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
    17221 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    17281 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    17341 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    17401 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
    17461 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    17521 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    17581 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    17641 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    17701 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    17761 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    17821 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    17881 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    17941 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
    18001 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
    18061 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
    18121 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
    18181 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
    18241 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
    18301 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    18361 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    18421 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
    18481 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    18541 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    18601 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
    18661 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    18721 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    18781 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    18841 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    18901 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
    18961 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
    19021 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
    19081 taatggcaag ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
    19141 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    19201 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    19261 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
    19321 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
    19381 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    19441 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
    19501 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
    19561 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
    19621 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
    19681 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
    19741 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
    19801 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
    19861 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
    19921 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
    19981 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
    20041 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
    20101 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
    20161 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
    20221 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
    20281 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
    20341 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
    20401 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
    20461 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
    20521 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcacg
    20581 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
    20641 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
    20701 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
    20761 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
    20821 caaaacgctt agcaaattga ttggctgccc aacgacaacg ttagccgaaa gcgtaagcca
    20881 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
    20941 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
    21001 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
    21061 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttgcagt cgatggggcg
    21121 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
    21181 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
    21241 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
    21301 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
    21361 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
    21421 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
    21481 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
    21541 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggtgcaggc
    21601 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
    21661 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
    21721 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
    21781 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
    21841 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
    21901 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
    21961 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
    22021 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
    22081 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
    22141 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
    22201 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
    22261 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
    22321 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
    22381 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
    22441 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
    22501 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
    22561 cacgatgaag cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg
    22621 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
    22681 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
    22741 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
    22801 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
    22861 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
    22921 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
    22981 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
    23041 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
    23101 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
    23161 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
    23221 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
    23281 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
    23341 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
    23401 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
    23461 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
    23521 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
    23581 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
    23641 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
    23701 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
    23761 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagagatc ggggaaccag
    23821 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
    23881 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
    23941 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
    24001 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
    24061 gttttgccgg accccataaa caacccagta ccaatggcac ctccaatggc aataagctga
    24121 acatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
    24181 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
    24241 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
    24301 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
    24361 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
    24421 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
    24481 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
    24541 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
    24601 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
    24661 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
    24721 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
    24781 cggaattgcg ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt
    24841 cacaccttct tttttggcgt tttcttccag gtatttcacg ccttcagcag ccatcgcctg
    24901 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
    24961 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag
    25021 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
    25081 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
    25141 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
    25201 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
    25261 ataataaagg cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg
    25321 aggaacaaga cgtggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc
    25381 tggagaaagt ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc
    25441 atcgtcgggg gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg
    25501 atgatacgcc caacgcgtcg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat
    25561 cacaaccgcc gacagaagaa cagccgcaaa ctacccaaac gcaaccattc cagccagata
    25621 gcggaattga taatcaatgg cgctcttacc gtgtcgaacc cggtaaaacg atggcgcagc
    25681 tgttccgcga ccacggactg cccgctaccg atgtctatgc aatggcgcag gtagaaggcg
    25741 cgggtaagcc gctgagtaat ttgcaaaatg ggcagatggt gaaaatccgc cagaacgcca
    25801 gcggcgtggt gacgggctta accattgata cggggaataa tcaacaggtc ctgtttaccc
    25861 gccagccgga tggcagtttt attcgtgcgc ggtagatgaa gggtcaacca aaagccagct
    25921 aaggattgtc tctttgatga ccttacgtct ggcttccagt gcctgggggg aaaccatact
    25981 gatatgataa actaggccaa gcgtatgctg gttgatcaaa taatatcccc caagcgcagc
    26041 aatattaata ttcacctgca atgggtcgat acctggctta aaaatattgt atttcttccc
    26101 ttcatccaat aaggattcca ttaactgcaa gtgagcatgg ttaatttcta agagacgctg
    26161 tgattttgca taatgcacac ctttactttg attttcacta tgcacaattt ttaaaaacca
    26221 aggattagca atataataat cccatgtaaa atcaataatt ttctcaatgg ctttttgtgg
    26281 cggaagatct gagaaatcaa gggccttttc tttttgtcga atatcgttcc aggtatattc
    26341 cagcacttcg ataaataaat tttctttatt accaaagtgg tgataaaccg tctgtttact
    26401 acaccctgct gcctttacaa tattatctac tctggcccct tcataaccat attcggcaaa
    26461 ttcattaacc gcacatagca gcaatttttc cttcaggctc tgtacgttgc tggtatcaat
    26521 atacataacg cttccccacg acctcttcgt ggtgactatc ataacctgat ttaatttcag
    26581 cttcagaaaa aagggcaaaa acttgtactt tcatcaggtt atcaaatgtc ctgtgatcgc
    26641 catcgcatat caaccaacta gatggttgat ctttttatcc tccagtcgca agctaatcat
    26701 caccaaggaa acaaaattca aaaaagagag ctattgtatg aaatccactc gtcctgttgc
    26761 cgtgattacc ggagccactc gcggtattgg aaagggctgt gcgcaggagc ttgctcgtgg
    26821 cgggtttaac ctgctgatta acgatcgtcc cgatgccgac agcgtagaaa aactgcatat
    26881 cacacaacaa gaatgtttgg cggaaggcgt ggaagtgatt tgcttccctg ctgatgtcgg
    26941 tgatctatct ctgcatgagg agatgctcga cgcggcgcaa aaccagtggg ggcggctgga
    27001 ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc ggtgacctcc tcgacctcga
    27061 accagacagc ttcgatcaaa acattgccat taacacccgc gcgccgttct tccttgctca
    27121 ggcattcagt aagcgcttac tggcacagcc gaaaccggaa gcggagctgc cgcaccgctc
    27181 gattattttc gtcagttcca tcaacgccat catgctggcg atgaatcgcg gcgaatacac
    27241 cattgccaaa accgccgttt ctgccgctgc tcgtctgttt gctgctcgcc tttgtaatga
    27301 acaaattggc gtttatgaag tgcgccctgg tctcatcaaa actgatatga ctattccggc
    27361 aacggcttat tacgacgaac tgattgccaa aggtctggtg ccgtggggac gttggggtta
    27421 tccggctgat attgcttcca ccgtccgcgc gatggcggaa ggtaaactga tttacacctg
    27481 tggtcaggcg gtcgcaatcg acggcggctt aagtatgccg cgcttctgag gctgaatcat
    27541 gagagatctg ttacaacgcc cggatttatt cagtatcaat accgcgacac tgggctataa
    27601 aacgccgcta cccgccatta ttgatgcctg cgccgcacgc ggtattggcg caattgcccc
    27661 ctggcgcagg gagttgcagg gtgaagatct ccagcaaatc acccgccagc ttgccgccag
    27721 taacatgagc gtttccggtt tgtgccgtag cacttactac accgcgccga cactggcgga
    27781 acgcaaactg gcgattgatg ataaccgccg ggcgctggac gacgcagcgg tgcttaacgc
    27841 cgcctgttat atgcaggtgg ttggcggtct gccaacgggc actaaagatc tatatgaagc
    27901 gcgagaacaa gtgaaacaag ggattcgcca gttgctgcca cattcgaaag atgtcggcgt
    27961 gccaattgcg ctggagccac tgcacccgat gacagctgcc gatcgctctt gcctgtgcac
    28021 gttacgtcag gcgctggact ggtgtgatga actcgatccc gatggtgagt ttggcctcgg
    28081 cgtggcggtg gatgtttatc acgtctggtg ggatccggat ctcgccagtc agatcctgcg
    28141 tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg ttaatgccga ccactgatct
    28201 ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat attccgtcaa ttcgtcggct
    28261 ggttgaaaac gccggattta atggtgctat tgaactggaa atattctcgc cttactggtg
    28321 gcagaaagat attaacag
//
LOCUS       DADLCQ010000049        27728 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000049 DADLCQ010000000
VERSION     DADLCQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.049"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..331)
                     /locus_tag="JQG95_001921"
     CDS             complement(<1..331)
                     /locus_tag="JQG95_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888481.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVTADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="JQG95_001922"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="JQG95_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY5888482.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="JQG95_001923"
     CDS             complement(1692..2072)
                     /locus_tag="JQG95_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY5888483.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2502)
                     /locus_tag="JQG95_001924"
     CDS             complement(2086..2502)
                     /locus_tag="JQG95_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888484.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQG95_001925"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="JQG95_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY5888485.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /locus_tag="JQG95_001926"
     CDS             complement(3503..3970)
                     /locus_tag="JQG95_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY5888486.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="JQG95_001927"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="JQG95_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY5888487.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="JQG95_001928"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="JQG95_001928"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY5888488.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..7650
                     /gene="rhtC"
                     /locus_tag="JQG95_001929"
     CDS             7030..7650
                     /gene="rhtC"
                     /locus_tag="JQG95_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY5888489.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7712..8323)
                     /gene="rhtB"
                     /locus_tag="JQG95_001930"
     CDS             complement(7712..8323)
                     /gene="rhtB"
                     /locus_tag="JQG95_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY5888490.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQLMQYIVLGVTTIVVDIIVMI
                     GYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8434..9456
                     /gene="pldB"
                     /locus_tag="JQG95_001931"
     CDS             8434..9456
                     /gene="pldB"
                     /locus_tag="JQG95_001931"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY5888491.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9464..10264
                     /gene="yigL"
                     /locus_tag="JQG95_001932"
     CDS             9464..10264
                     /gene="yigL"
                     /locus_tag="JQG95_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY5888492.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10340..11239
                     /gene="bioP"
                     /locus_tag="JQG95_001933"
     CDS             10340..11239
                     /gene="bioP"
                     /locus_tag="JQG95_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY5888493.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11127..12080)
                     /gene="metR"
                     /locus_tag="JQG95_001934"
     CDS             complement(11127..12080)
                     /gene="metR"
                     /locus_tag="JQG95_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY5888494.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12316..14577
                     /gene="metE"
                     /locus_tag="JQG95_001935"
     CDS             12316..14577
                     /gene="metE"
                     /locus_tag="JQG95_001935"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY5888495.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14616..15431)
                     /locus_tag="JQG95_001936"
     CDS             complement(14616..15431)
                     /locus_tag="JQG95_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY5888496.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15693..16454
                     /gene="udp"
                     /locus_tag="JQG95_001937"
     CDS             15693..16454
                     /gene="udp"
                     /locus_tag="JQG95_001937"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY5888497.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16595..18022
                     /gene="rmuC"
                     /locus_tag="JQG95_001938"
     CDS             16595..18022
                     /gene="rmuC"
                     /locus_tag="JQG95_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY5888498.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18117..18872
                     /gene="ubiE"
                     /locus_tag="JQG95_001939"
     CDS             18117..18872
                     /gene="ubiE"
                     /locus_tag="JQG95_001939"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY5888499.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18886..19491
                     /gene="ubiJ"
                     /locus_tag="JQG95_001940"
     CDS             18886..19491
                     /gene="ubiJ"
                     /locus_tag="JQG95_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY5888500.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19488..21128
                     /gene="ubiB"
                     /locus_tag="JQG95_001941"
     CDS             19488..21128
                     /gene="ubiB"
                     /locus_tag="JQG95_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY5888501.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21207..21476
                     /gene="tatA"
                     /locus_tag="JQG95_001942"
     CDS             21207..21476
                     /gene="tatA"
                     /locus_tag="JQG95_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY5888502.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21480..21995
                     /gene="tatB"
                     /locus_tag="JQG95_001943"
     CDS             21480..21995
                     /gene="tatB"
                     /locus_tag="JQG95_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY5888503.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21998..22774
                     /gene="tatC"
                     /locus_tag="JQG95_001944"
     CDS             21998..22774
                     /gene="tatC"
                     /locus_tag="JQG95_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY5888504.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22816..23598
                     /gene="tatD"
                     /locus_tag="JQG95_001945"
     CDS             22816..23598
                     /gene="tatD"
                     /locus_tag="JQG95_001945"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY5888505.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="JQG95_001946"
     CDS             complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="JQG95_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY5888506.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24250..25743
                     /gene="ubiD"
                     /locus_tag="JQG95_001947"
     CDS             24250..25743
                     /gene="ubiD"
                     /locus_tag="JQG95_001947"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY5888507.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGEHFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25789..26490
                     /gene="fre"
                     /locus_tag="JQG95_001948"
     CDS             25789..26490
                     /gene="fre"
                     /locus_tag="JQG95_001948"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY5888508.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26682..>27728)
                     /gene="fadA"
                     /locus_tag="JQG95_001949"
     CDS             complement(26682..>27728)
                     /gene="fadA"
                     /locus_tag="JQG95_001949"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY5888509.1"
                     /translation="ARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVP
                     AVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVA
                     KAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADG
                     VLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAH
                     ELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQI
                     LPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMC
                     IGLGQGIATVFERV"
BASE COUNT         6604 a         6943 c         7479 g         6702 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggtaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tagatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaaa taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaataaaaat atatatcggt gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg taccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagta cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccca ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca ggccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gcgaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aggggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc atttgattat cgaaaaaatg gcctggctgc atacgctgat tatggtaggc
     7261 ggtggcctgt atctctgctg gatgggttac cagatgctac gcggtgcact gaaaaaagag
     7321 gctgtatctg cgcctgcacc acaggttgag ctggcgaaaa gcgggcgcag tttcctgaaa
     7381 ggcttactga ccaatctcgc caatccgaaa gcgattatct attttggttc ggtattctca
     7441 ttgtttgtcg gcgataacgt tggcactacc gagcgctggg gcattttcgc gttgattatt
     7501 gtcgaaacac tggcgtggtt taccgtcgtt gccagtctgt ttgccctgcc gcaaatgcgc
     7561 cgtggttatc aacgtctggc gaagtggatt gatggttttg ccggggcgtt atttgccgga
     7621 tttggcattc atttgattat ttcgcggtga tgccagacgc gtcttcagag taagtcggat
     7681 aaggcgttta cgccgcatcc gacattattt ttcacgcatg cctcgccgat gctaacagcg
     7741 ctcccaccag cataaacaac gagccgaaaa tcttattcag cgccttcatc tgctttggtc
     7801 ctttaatcca tagagcaatc ctttgagcaa gggtggcgta accgatcatc acaataatat
     7861 cgaccacaat agtggtgacg ccgagcacga tatactgcat cagttgcggc atgatgaatt
     7921 gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc acaaaaactg
     7981 cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga tcaattgcgc
     8041 cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct gcccacttta
     8101 acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg ccaaccagca
     8161 caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg ccgcgataac
     8221 cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac aggcttaaaa
     8281 tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac tcccggtgtg
     8341 tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg cttaaaaaaa
     8401 cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa agactgggaa
     8461 acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt ctggcgtcag
     8521 cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt ccgttttcgc
     8581 gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag ctacgtaaaa
     8641 tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat catcgaccat
     8701 cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca tgttaatcgc
     8761 tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca gcccggtccg
     8821 tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac attatttctg
     8881 caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt tgggatcgtg
     8941 attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc gcatccacgt
     9001 ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt tgctatcaac
     9061 gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc tgatgaccca
     9121 acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct ggctggcgaa
     9181 caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca agctgaagag
     9241 gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac cgccgcgggc
     9301 catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga gatccttttt
     9361 gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt caacaggcat
     9421 aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc aggttgttgc
     9481 gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt acgccaaaga
     9541 aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg gtcgtcacca
     9601 cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga ttacctccaa
     9661 tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc tggatcgcga
     9721 cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta ctaacgttta
     9781 tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct tttttaaaga
     9841 agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag gcgtcagcaa
     9901 agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg cgattaacgc
     9961 tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg aagtgatggc
    10021 gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg gctacagcct
    10081 gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt cgatggcggg
    10141 gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc cggagctgga
    10201 agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac tctatttatc
    10261 gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg gatactcgtt
    10321 aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt gggccttctc
    10381 ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg cggtgctggt
    10441 gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta gcaatagcct
    10501 gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga tgtatatgct
    10561 gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca ccgtactgac
    10621 gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc gctggggcta
    10681 tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg atcaggtcac
    10741 cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt ttgccatcgg
    10801 catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata acgcctttgc
    10861 gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc tgggaaatgc
    10921 gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg gcgtggtggc
    10981 ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg ccggaacgct
    11041 gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg ctatctggca
    11101 ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg cctcactgtg
    11161 ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc gcaggcgtga
    11221 ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc atcgcgcacg
    11281 gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac cagaccctgg
    11341 cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg cgcggcaacc
    11401 atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac gcctgccggc
    11461 tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat taatagcgtc
    11521 tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg gtcaggtgct
    11581 aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact gcgcggcagg
    11641 atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg cgggtcaaac
    11701 gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa attttctaaa
    11761 gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag acgcgtctgc
    11821 tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg gtttgccagt
    11881 tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg cacaaatagc
    11941 cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga ttgcgtctga
    12001 tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg tagcgttttc
    12061 aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt gcgcttgagg
    12121 aatatacagt aaccgctaat tatggatgtg taaacatctg gacggctaaa atcattcgtc
    12181 ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact tcagtaagct
    12241 cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga tttacatata
    12301 attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg cgttggcctg
    12361 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
    12421 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
    12481 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac caccagtttg
    12541 ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
    12601 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    12661 accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa aggccaacag
    12721 ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc gctgagccac
    12781 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    12841 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    12901 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    12961 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    13021 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    13081 gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa cgttgttgaa
    13141 ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa tggtcgtaac
    13201 gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    13261 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    13321 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    13381 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    13441 gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt
    13501 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa
    13561 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    13621 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac
    13681 aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg
    13741 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    13801 gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    13861 tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat taccgtggag
    13921 tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct gacggggccg
    13981 gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    14041 cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg aattggcatc
    14101 atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat
    14161 gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    14221 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    14281 gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    14341 tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga cattcactcg
    14401 ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc aaaacgcatt
    14461 ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggccagaa
    14521 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg ggggtaaatc
    14581 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    14641 tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    14701 gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat ctctgctttc
    14761 gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg aatgctgtta
    14821 tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    14881 tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc cactgcggct
    14941 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    15001 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    15061 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    15121 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    15181 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    15241 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    15301 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    15361 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    15421 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    15481 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    15541 agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    15601 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    15661 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    15721 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    15781 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    15841 tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    15901 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    15961 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    16021 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    16081 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct attggcgcga
    16141 caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag gaacgttacg
    16201 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    16261 tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt gcaagtcagg
    16321 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    16381 atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc
    16441 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    16501 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    16561 tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca tggtttacgc
    16621 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aacatgcgca
    16681 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    16741 acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca ataacgaagt
    16801 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    16861 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    16921 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    16981 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    17041 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    17101 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    17161 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    17221 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    17281 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    17341 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    17401 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    17461 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    17521 ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg accgccagcc
    17581 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    17641 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gtaacgccca
    17701 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    17761 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    17821 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    17881 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    17941 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    18001 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    18061 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    18121 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    18181 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    18241 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    18301 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    18361 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    18421 aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    18481 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    18541 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    18601 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    18661 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    18721 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    18781 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcagggg
    18841 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    18901 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    18961 aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    19021 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    19081 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    19141 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    19201 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    19261 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    19321 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    19381 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    19441 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    19501 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    19561 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    19621 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    19681 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    19741 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    19801 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    19861 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    19921 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gtcattaaag
    19981 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    20041 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt gatgaactga
    20101 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    20161 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    20221 gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc actaacatga
    20281 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    20341 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    20401 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    20461 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    20521 tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    20581 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    20641 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    20701 tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg cagctcgatt
    20761 tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    20821 ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    20881 ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    20941 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    21001 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gagtgggggc
    21061 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    21121 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa
    21181 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    21241 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    21301 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    21361 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    21421 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    21481 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    21541 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    21601 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    21661 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt
    21721 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    21781 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    21841 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    21901 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    21961 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    22021 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    22081 cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg tatccgcgcc
    22141 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    22201 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    22261 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    22321 gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg cctactttgt
    22381 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    22441 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    22501 ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct cgtcagaaga
    22561 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    22621 gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc tgtttgaaat
    22681 cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    22741 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    22801 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    22861 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    22921 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    22981 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    23041 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    23101 tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc tcagctacgc
    23161 attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    23221 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    23281 ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg cattaccggt
    23341 tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt gattccggcg
    23401 gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct cacgccaaag
    23461 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    23521 tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt caaaacactg
    23581 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    23641 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    23701 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataagggggg
    23761 gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac cgccgatggg
    23821 actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt agcgctgatg
    23881 gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg gaataacggt
    23941 tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat cggagcgagg
    24001 caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag
    24061 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    24121 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    24181 gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg cgcagattaa
    24241 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac gctgcttgaa
    24301 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    24361 attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    24421 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    24481 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    24541 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaggtg
    24601 ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    24661 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    24721 ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca gaatctgggc
    24781 atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    24841 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaaca tttcccggtt
    24901 tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc cgttccggat
    24961 acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga agtagtaaag
    25021 tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga agggtatatc
    25081 gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta ctataatgaa
    25141 gtcgatagtt tcccggtgtt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    25201 cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt ggcgttgaac
    25261 gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    25321 ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta cgccggacac
    25381 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaaattt
    25441 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    25501 accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    25561 ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga tgccacgaat
    25621 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    25681 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    25741 tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    25801 agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt ccgcatcgtg
    25861 ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat ggatgagcgc
    25921 gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat cgagctgcat
    25981 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    26041 catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    26101 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    26161 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    26221 catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg tctgcaagtg
    26281 gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac cgtgttaacg
    26341 gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc cggacgtttt
    26401 gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc
    26461 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    26521 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatt
    26581 ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgccggatc
    26641 ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc
    26701 gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg
    26761 cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc cagcggatga
    26821 cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc cattagcccg
    26881 agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg
    26941 atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg
    27001 taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg agcgcgcggc
    27061 ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg
    27121 gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa cgccggacgc
    27181 agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc
    27241 ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt aaatgcggcc
    27301 gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca
    27361 cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct
    27421 ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg
    27481 cctatatgct ccacgccgcc aaccagacat gcctgcgcat cgccagtcat gatcattcgt
    27541 gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg
    27601 acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa accctgctcc
    27661 agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg
    27721 ttacgcgc
//
LOCUS       DADLCQ010000050        27653 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000050 DADLCQ010000000
VERSION     DADLCQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.050"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            2..109
                     /locus_tag="JQG95_001950"
     CDS             2..109
                     /locus_tag="JQG95_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY5888510.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(196..1875)
                     /gene="bcsG"
                     /locus_tag="JQG95_001951"
     CDS             complement(196..1875)
                     /gene="bcsG"
                     /locus_tag="JQG95_001951"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY5888511.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1872..2063)
                     /gene="bcsF"
                     /locus_tag="JQG95_001952"
     CDS             complement(1872..2063)
                     /gene="bcsF"
                     /locus_tag="JQG95_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY5888512.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2060..3631)
                     /gene="bcsE"
                     /locus_tag="JQG95_001953"
     CDS             complement(2060..3631)
                     /gene="bcsE"
                     /locus_tag="JQG95_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY5888513.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3904..4092
                     /locus_tag="JQG95_001954"
     CDS             3904..4092
                     /locus_tag="JQG95_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888514.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4104..4856
                     /gene="bcsQ"
                     /locus_tag="JQG95_001955"
     CDS             4104..4856
                     /gene="bcsQ"
                     /locus_tag="JQG95_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY5888515.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLCYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4853..5212
                     /locus_tag="JQG95_001956"
     CDS             4853..5212
                     /locus_tag="JQG95_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY5888516.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5209..7470
                     /gene="bcsA"
                     /locus_tag="JQG95_001957"
                     /pseudo
     CDS             5209..7470
                     /gene="bcsA"
                     /locus_tag="JQG95_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7481..9820
                     /gene="bcsB"
                     /locus_tag="JQG95_001958"
     CDS             7481..9820
                     /gene="bcsB"
                     /locus_tag="JQG95_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY5888517.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISSRRLNPDNE"
     gene            9827..10933
                     /gene="bcsZ"
                     /locus_tag="JQG95_001959"
     CDS             9827..10933
                     /gene="bcsZ"
                     /locus_tag="JQG95_001959"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY5888518.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10992..11168
                     /locus_tag="JQG95_001960"
                     /pseudo
     CDS             10992..11168
                     /locus_tag="JQG95_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            11496..13244
                     /gene="bcsC"
                     /locus_tag="JQG95_001961"
                     /pseudo
     CDS             11496..13244
                     /gene="bcsC"
                     /locus_tag="JQG95_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            13326..15314
                     /gene="hmsP"
                     /locus_tag="JQG95_001962"
     CDS             13326..15314
                     /gene="hmsP"
                     /locus_tag="JQG95_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY5888519.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNFSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            15499..16785
                     /locus_tag="JQG95_001963"
     CDS             15499..16785
                     /locus_tag="JQG95_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY5888520.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17006..18502
                     /locus_tag="JQG95_001964"
     CDS             17006..18502
                     /locus_tag="JQG95_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY5888521.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(18598..19746)
                     /locus_tag="JQG95_001965"
     CDS             complement(18598..19746)
                     /locus_tag="JQG95_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY5888522.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            19759..20526
                     /gene="pdeH"
                     /locus_tag="JQG95_001966"
     CDS             19759..20526
                     /gene="pdeH"
                     /locus_tag="JQG95_001966"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY5888523.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            20596..22656
                     /locus_tag="JQG95_001967"
     CDS             20596..22656
                     /locus_tag="JQG95_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY5888524.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(22838..24160)
                     /locus_tag="JQG95_001968"
     CDS             complement(22838..24160)
                     /locus_tag="JQG95_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY5888525.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(24571..25584)
                     /gene="yhjD"
                     /locus_tag="JQG95_001969"
     CDS             complement(24571..25584)
                     /gene="yhjD"
                     /locus_tag="JQG95_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY5888526.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(25633..26604)
                     /locus_tag="JQG95_001970"
     CDS             complement(25633..26604)
                     /locus_tag="JQG95_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5888527.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            27052..>27653
                     /locus_tag="JQG95_001971"
     CDS             27052..>27653
                     /locus_tag="JQG95_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5888528.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVQVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         6583 a         6976 c         7579 g         6515 t
ORIGIN      
        1 tatgacgctc gcagaactgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc
       61 tggcattctt gccagtatga tcgtgaactg gctgaacaag cggaagtaac gtgtcatgcg
      121 ggcgtcaggc tgccgtaatg gcaatttgcg cccggatcag gccgcagagg ttaaactctg
      181 cggccttttt cgtgcttact gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta
      241 cggtttatct tggtattgaa taactactgc atttgagttc tcggagaccg gtgctgtttg
      301 tggcaaccca ctggtgagtt ttttccagtc aacattgtct tcggtgaaaa tcttgccatc
      361 aagaacgcga accaccagat cggagatagc caggaagctg ctcggttggt caatgacaat
      421 cggtgccccc tgatgtggtg ccttcatgcc gaagaatttc accccaacgg ggacgtctgt
      481 gatagacggg ctagggatat cacgtaggcc agatacctgc attctgtcgc ccttcagcgc
      541 gccgccgtgt tccggcacca cgaccaccat cactttacga cccgatttct ccagttcagt
      601 aaagaaggcg tccagttcat caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt
      661 gctgaccccc ggataatggt tgccgtcatg cagtggaagc gtgttgtaga acgtggcact
      721 acggctgttt ttatcttttt cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc
      781 ataaaccggc gaaccatcaa agcccagcaa aataaccggc agatttgttt gatccatcaa
      841 ttcagtctgc atgccgccat tttcgcgaac ttctttcaaa aaaccgccga actggccgtt
      901 atgtcccatc atcaggtgct gggtaaagcc cagtttcgaa aggttatcaa acagatagca
      961 gtcgttattt gccggttgat acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag
     1021 taaacggatc gccgccgggc cactgtagga ggtggcggag ttaaagttct tgaaatcaat
     1081 atcgaaatgc gaccacagtg gatgcgacat caacccggcg gcttctatat ccgaccagga
     1141 aagcgaacag atgttaatca ccagtagttc aaatggctga gcatcagcgg gcagcgaaga
     1201 cgggaaggtc gatttacgtt tcgcctccgc gttatagaaa ttattcagcc aggcgttaag
     1261 gttcgccgtt gttggcggtg cagtttgtgc gggcatatca cccactaccg gtgcgccacc
     1321 cgtcgccgca acggttgccg ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg
     1381 tccggctggc cacaaggaga aacttggtcc cgccaaggta agtacgttca gccatagcag
     1441 tatggcaacc acaaaaacgg taatgcgaat ccattgtgac aggaataacc aggccactaa
     1501 taaaacaaaa atggccccaa tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa
     1561 ataatcggta ctgaaccccg ccacctgcga accctggctc attatgcttt ccgggccagg
     1621 caaccagatg tcatgccaga acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa
     1681 gcgatgcagg ctgtaacgcg gaatgggcat cagcagaaac gcggcaaaca ccaaattgag
     1741 gagcgggtgg aagttaagat atcccgccca caacaggcca aacttaacca gaaaatagaa
     1801 gttccagccg gaaaggccgc gccagtattg ccagagggaa gaaggcatgg cggtattttg
     1861 cgtaaattga gtcatttttt ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc
     1921 ggtttaacat aacgaggttt agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa
     1981 gagtgccgcg ccagatagcc cagcgggaaa aatatcagtg cgcaaacgat aataatttca
     2041 atgatatcgc tgatggtcat catgatgagc gctccacagc atcatctaac agtcgcatgg
     2101 gttctggtat tcgtcgccag tggcgaccat cgtgctcggc attgatgacc ggtttagaac
     2161 tttgcgttaa aggcagcggc attccccatt gttctggggc aagcaggcgc atctgcacca
     2221 gctcggcact gatttgatca tcttcaaacc agaccatacg gtttgagaaa atgtcgccag
     2281 taggcaatgg gaaaatatga ttcaacgcgg tatccagatc gttaatccga cagaatgaga
     2341 gaaacagcac cagccgatta ccgccaatgg tcatgatatc gccggtacgg ttagggcgac
     2401 atagcgtcag ggcttgctca acgcggatac ccggtaccgg acgtagggca accagaacgc
     2461 ctttaccgtg ggcaggcaag agagggttat tcatcatgtt gttgacggca ttacagaaca
     2521 catcccactt ctggaaacca cgcagtttga gcggctgggt cattgacagc aaggtagtga
     2581 tatcttccgg tacatagcga ctaaacttct gcccttgcac gctttcgatc atcgtcagac
     2641 aacgggagag tggcgcattc cacggaataa ccatatttgc accgcaggcc aataacaaac
     2701 gttcatcggt ggcgcgcagg ctagcggtat tttcccgcac gaggattttc atcgcactac
     2761 cgcgctggcg acgcagggta tgaatgctgc gggccagtgg ctcgatttgc gcattttgct
     2821 gtaaagaaaa gaccaccgtc gccgcctgag cggtacgggc ttcattgaac aggacttcgt
     2881 tattgttgaa cagttgccag tgttccgata gcggcggcgc accttccagt acagcaacat
     2941 tactcagaat gcgtttttcg tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta
     3001 atgtccagat accattttgt tgctgaacgc taagctgctg acgggcgctg accccttttt
     3061 cgttgcacca gaaggcaata tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa
     3121 gaccaaaaag tgaacggtat tcctcaagca ataatgaaaa ttgtttatcg ttattatttc
     3181 cgggattaat taccaaaagc gaacaatggt ttaacctgct ccatttattc attttatcca
     3241 accatgagcg aagccgctcg gcaggaatat tttgccatgc gttatttgcg caaacaagaa
     3301 taaaaaggta attatgggga tcaatagaac actgcaaatc acgggccaaa tagtatagac
     3361 ctttttcatg atttagcatt gaaaataatt ttattttttc cggcccttga gaatcatcta
     3421 attgaaagat tttcgccgga tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag
     3481 cctgggatgc aattgtttga ttcgccagac tgatagcatc ttcatggcga tcgacgttaa
     3541 accaccagac gccgcctgct ggcatatgtc gcagctcatc ccataatgat gagataccga
     3601 tagagaatac agggtccaca atgtccctca tgtcaccatt ttgtctgatt atcagtttac
     3661 tagcgaaagc taagaaataa acctaccatt gaaattaaaa cttatcagat ttagcatgtg
     3721 aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta actttttctg
     3781 ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac aataaaccgg
     3841 cgaaatgctc acggttagaa ttgtctcatg aatggtacgg ttatttcata gggatcaagc
     3901 aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta tatcttccag
     3961 aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta tgccgatatt
     4021 tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc agagtttgcg
     4081 caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg cggggaggcg
     4141 tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg ggagaaaatg
     4201 tcctggtggt cgatgcctgc ccggacaact tattgcgcct gtcatttaat gttgatttta
     4261 cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt gacgctgggt
     4321 tgtgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt gaagaacaag
     4381 aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta cagcaactaa
     4441 aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc tcgcagataa
     4501 cccaccagct gctgagtttg tgcgagcact cgctggcaat cgtcaatgtg gatgccaact
     4561 gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg attaatgact
     4621 tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa agccagcgcc
     4681 gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg gcggctaagc
     4741 aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg acgctggcga
     4801 actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca tcatgagtat
     4861 cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc gttatcgcga
     4921 ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct ggatgatcct
     4981 ggcctggatt tttattccgc tggagcaccc gcgctggcag cgtattcgcg cagaacataa
     5041 aaacctgtat acgcatatca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct
     5101 cattcaaaca tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag
     5161 cgggcatttt caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg
     5221 cctgagcgcg ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg
     5281 agtgccgggg cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg
     5341 ttaatctgcg ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg
     5401 tggggggtag cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt
     5461 gtgctgtcgc tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac
     5521 tgggacgatc cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg
     5581 tggattgtgc tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg
     5641 ccattgccga aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac
     5701 gaagatctca atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa
     5761 gacaaactga acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg
     5821 caaaacgtgg gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac
     5881 atcaacaatg cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac
     5941 gtaccaacgc gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg
     6001 gcgatgatgc agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga
     6061 cgttttcgta aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac
     6121 gatatgtggg acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg
     6181 gatgaaattg gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt
     6241 ttgcaccgtc gtggctatac ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg
     6301 accgaaagtc tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtataa
     6361 atcttccgtc tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc
     6421 tacgtcaacg ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg
     6481 ccgctggcgt tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc
     6541 ctattcgtgc tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa
     6601 tatcgccact ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca
     6661 ccgacgctgg tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt
     6721 ggactggtgg aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt
     6781 ctactcaacc tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc
     6841 gagatgctca ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc
     6901 ggcgcagttg cggtatcggt agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg
     6961 acgatgcccg cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat
     7021 ttctccgacg gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag
     7081 aaagtgaatc tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg
     7141 cgcgtgatgg gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc
     7201 gattttgtgc agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac
     7261 ccggaagata agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac
     7321 cgccatctgg cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct
     7381 ctggtttcct gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa
     7441 ccatcggatc aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg
     7501 gatttgtgca gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc
     7561 agcaacgcaa ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc
     7621 gcaggtgggg caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa
     7681 cggtccttcg cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat
     7741 ggtgctacgt ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt
     7801 ggtgacgaag gcgatgctca acttcgaata cacgccatcg ccatcgttac tgcctgtcca
     7861 gtcgcagtta aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga
     7921 acagttgggt aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt
     7981 caaccgtgtg cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag
     8041 caccacgctt tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct
     8101 gaatgtgaag aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg
     8161 tactaacacc ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc
     8221 tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt
     8281 gctctataac caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc
     8341 ggacttcctg cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc
     8401 gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca
     8461 ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa
     8521 tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga
     8581 tcgtccggtc acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct
     8641 tgagccagca gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag
     8701 taccggcatt gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc
     8761 gcggatggat atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca
     8821 ggaggcgaac cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac
     8881 agatgtctct attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga
     8941 gtatatgaac ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca
     9001 gaatcatgtg gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat
     9061 cccgatgccg gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga
     9121 tctgtcgcaa accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt
     9181 gctgaatact gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt
     9241 gaccgatgat ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat
     9301 cccggacaaa ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg
     9361 ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg
     9421 cgcggcagaa acccagtcaa ctctgacctc ttccggtgcg atggcggcgg tgattggctt
     9481 ccagtcgccg tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg
     9541 ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc
     9601 ggtcgcggtg atccgcgagt ccggtatcaa cagcctacgt gttggcgacg tttattacgt
     9661 aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct
     9721 ggcggtgctg gcgactatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg
     9781 aattattagt agtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg
     9841 tagtggaatc gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg
     9901 gcctgcctgg gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc
     9961 cagcgacgcg cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct
    10021 ggcggctaac gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc
    10081 tcagggttct ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa
    10141 gtgggaagtg ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt
    10201 gctggaggcg gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct
    10261 aaaacgtatc gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc
    10321 gggcaaagtg ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc
    10381 gacgctggcg cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa
    10441 tcaacattta ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga
    10501 gaaagacaaa ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat
    10561 ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct
    10621 caaccggttt aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt
    10681 ggatgtggct acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct
    10741 gcctttttta caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt
    10801 tcccggcagc gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca
    10861 acaccgtttc cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc
    10921 aaattcacac taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag
    10981 gcagcaccaa ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg
    11041 ggctggcgaa tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc
    11101 tctctgccag ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc
    11161 tggcacagca ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc
    11221 agcggcaacg actggcgctg ggccccggca gcgtatggat tacttaccga ctttcgcagg
    11281 atctctggca ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc
    11341 agaagccgaa caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc
    11401 aggacagagc ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata
    11461 ttcaggagct ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc
    11521 gagaaagcgg caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc
    11581 gtattgacct cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca
    11641 ctgcatatca gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga
    11701 cggaagtgga tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc
    11761 ctgccaccga taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc
    11821 ttggagatac cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc
    11881 agccaccgtc gatggaaagc gcgatggtac tgcgcgacgg tgcgaagttt gaagcgcagg
    11941 cgggcgatcc aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga
    12001 ctacgacgcg tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag
    12061 atgactggct gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc
    12121 ttaacgtcac ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc
    12181 tgaaagcgca cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct
    12241 ttcgcagtga tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat
    12301 gggatgacaa ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg
    12361 attccggtgc cagcgtggcg gtcggctggc gaaatgacgt ctggagctgg gatatcggta
    12421 ccacgccgat gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata
    12481 tcgggccgct gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg
    12541 cctttggtgg gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg
    12601 ccgacggtgt ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat
    12661 cgcttagtgg cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga
    12721 tgacgggcta ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga
    12781 acaacatgat ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt
    12841 actacagtcc gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg
    12901 aaaactggtc gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca
    12961 tgccgcgtta tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc
    13021 aatccaacga tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac
    13081 gacgtgttac ttccaactgg tttgttggta cggcaattga tatccagcag gcgaaagatt
    13141 acgcacccag ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca
    13201 tggatttacc gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc
    13261 tctcttaatg ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag
    13321 acaatttgcg cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg
    13381 tcctggtgtt cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc
    13441 gctataacac ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt
    13501 cagctatttt gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg
    13561 caggcgtggt cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa
    13621 gttttattcc agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc
    13681 aaatctcgct gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc
    13741 tggtgctaca ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt
    13801 tagtgaccat ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta
    13861 accgcctgat tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc
    13921 aggagcttgt tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta
    13981 tgttggtgcg cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga
    14041 acgaaaatgc gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga
    14101 tgctggagca ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa
    14161 ccctgcgtga tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc
    14221 tggtggaaaa actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt
    14281 atgactttgc tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc
    14341 agcaagtact cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc
    14401 actgtagcat tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc
    14461 gcgctatttc tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg
    14521 atccgcagca gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg
    14581 cactggaaaa tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta
    14641 aactggtcag tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc
    14701 cggatggctt aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg
    14761 tgctggaaga gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct
    14821 tgtcggtaaa cttctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg
    14881 aactgttaac ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc
    14941 gacgtattga cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag
    15001 ttcgggtggc gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata
    15061 tgaaatcgtt gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg
    15121 aagatagcag catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga
    15181 ttgccgaagg cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta
    15241 ttgcccaggg cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc
    15301 tggaagaaaa gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg
    15361 agccagctca aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat
    15421 gttgtctggg tgttatttta aggccgcagg taccccccat aaccttacaa gacctgtggt
    15481 tttactaaag gacaccctat gaaaacctct ctgtttaaaa gcctttactt tcaggtcctg
    15541 acagcgatag ccattggtat tctccttggc catttctatc ctgaaatagg cgagcaaatg
    15601 aaaccgcttg gcgacggctt cgttaagctc attaagatga tcatcgctcc tgtcatcttt
    15661 tgtaccgtcg taacgggcat tgcgggcatg gaaagcatga aggcggtcgg tcgtaccggc
    15721 gcagtcgcac tgctttactt tgaaattgtc agtaccatcg cgctgattat tggtcttatc
    15781 atcgttaacg tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac gcttgatgcg
    15841 aaagcggtag cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc cttcattatg
    15901 gatgtcatcc cggcgagcgt cattggcgca tttgccagcg gtaacattct gcaggtgctg
    15961 ctgtttgccg tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg ccaactgatt
    16021 tttaacgtga tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat gatcatgcgt
    16081 ctggcaccta ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata cggcgtcggc
    16141 acactggtgc aactggggca gctgattatc tgtttctaca ttacctgtat cctgtttgtg
    16201 gtgctggtat tgggttcaat cgctaaagcg actggtttca gtatcttcaa atttatccgc
    16261 tacatccgtg aagaactgct gattgtactg gggacttcat cttccgagtc ggcgctgccg
    16321 cgtatgctcg acaagatgga gaaactcggc tgccgtaaat cggtggtggg gctggttatc
    16381 ccgacaggct actcgtttaa ccttgatggc acatcgatat acctgacaat ggcggcggtg
    16441 tttatcgccc aggccactaa cagtcagatg gatatcgtcc accaaatcac gctgttaatc
    16501 gtgttgctgc tttcttctaa aggggcggca ggggtaacgg gtagtggctt tatcgtgctg
    16561 gcggcgacgc tctctgcggt gggccatttg ccggtagcgg gtctggcgct gatcctcggt
    16621 atcgaccgct ttatgtcaga agctcgtgcg ctaactaacc tggtcggtaa cggcgtagcg
    16681 accattgtcg ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga cgatgtgctg
    16741 aataatcgtg cgccggatgg caaaacgcac gaattatcct cttaatctca ccacttatgc
    16801 ccgcactccc ttccccgagt gcgggtgttt gcgcataatt gacccctgtg cctgaactta
    16861 ccacctgggt gagttcaggt ttatttcact tcttccgtga cattttcatg ttcttgcggt
    16921 ctaacacgaa gtgtttttaa cgtcatttag actgaccgac aacgggcagt ttttttatta
    16981 ccaggatagt tgatcagggg ttcacatgca gggcataaaa attcgacttt tagcgggcgg
    17041 tttgctgatg atggccactg ctggctatgt gcaggcagat gcgctccagc ctgatccagc
    17101 atggcaacag gggacgcttt ccaacggttt acagtggcaa gtgctgacca ccccccagcg
    17161 tcccagcgat cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac
    17221 acaacagagc ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct
    17281 tgacgcagca caggcgcgtt cattgtggca gcaggggatc gaccctaaac gcccgatgcc
    17341 gccggtaatt gtctcttatg acaccacgct gtttaatctg agtttgccca ataaccgtaa
    17401 cgatttgctg aaagaagcgc tctcttatct ggcaaatgcc actggcaaat tgaccatcac
    17461 accagaaacc atcaaccacg cgctgcaaag tcaggacatg gtggcaacct ggcctgccga
    17521 tactaaagag ggctggtggc gctatcgtct gaaagggtca accttgttag gtcacgatcc
    17581 tgccgatccg ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg
    17641 gtacaccccg gatgcaatga cgctactggt ggtgggaaac gtggatgcgc gctcggttgt
    17701 cgaccaaatc aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt
    17761 gccgacgctt tctccgctgc gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca
    17821 ggaccggtta tctatcatgt gggatacgcc gtggcagccg attcgtgaat cagccgcact
    17881 gctgcgctac tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg ttcagcaagc
    17941 gttaagcgcc agtgacagca aagacatcgg tcttggattt gactgccgtg tgctgtatct
    18001 gcgtgcgcag tgtgccatca acatcgaatc accaaacgac aagctgaaca gcaaccttaa
    18061 tctggtggcg cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag aagagttcaa
    18121 tgcgttagtg gcgcaaaaga aactggagct gcagaaactg tttgccgcct atgcacgggc
    18181 tgataccgat attctgatgg gccagcggat gcgttcgttg caaaatcagg tcgtcgatat
    18241 cgcgccggag cagtatcaga aactgcggca ggatttcctt aacagcctga ctgtggagat
    18301 gttaaatcag gatctgcgtc agcagttgtc gaatgatatg gcgttaatac tgctgcagcc
    18361 gaaaggcgag ccggaattta acatgaaagc gttgcaggcg gcctgggatc aaatcatggc
    18421 cccatctacc gccgctgcga ccacctctgt cgccacggat gacgtacatc ctgaagtgac
    18481 ggatattcca cctgcacagt aatgcctgat ggtgatgagt tagcacaacc atcaggtatt
    18541 tatggatgag ctggatagag gggtaacact ttatccagcc ttttgcatat gctgcgttta
    18601 cgctggcatc gcctcacgcg ggataatcgc gccgcgatac tgaataacgg tacttgcggt
    18661 caggtgcccg cgtttcgccg cgttttccgc actgccgccc gtcaaacgta ccgccagata
    18721 accggcgctg aaagagtcgc cagctgcggt ggtatcgatc actttttctt tcggcagttt
    18781 caccgccgga acatcaacta acgcttcacc agcaatggac accaggcaag aatccgcccc
    18841 gcgtttcacc accacttctt tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac
    18901 cggctgttga ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga tatccgtgca
    18961 ttccagcatt tgctggtaca cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg
    19021 atagttattg tcgaaaatca cttttccgcc tttggcgcgg cattcgcgca gcagggaaag
    19081 cagcttttcg cggctggtcg ggcttaagat cgccaggcta atcccgctca ggtagagata
    19141 atcgaaattc gccagctctt cgcaaatcgc cgcagactgc tcgctcgcca gccagaattt
    19201 ggcggcggct tcgttacgcc agtagtagaa cgtacgttcg ccggtgctgt cggtttcaat
    19261 gtagtaaagg cccggaagac ggttttccat ccgttgggtc agggaagtat caacgttctc
    19321 gccgtgccag gcgtccagca tctgctggct aaaactgtcc gttcccagcg ccgttacgta
    19381 atgaacggtt aatgccgcag gatcgacctg acgggcgata tagacggaag tgttcagggt
    19441 atcgccgccg aaaccgcgct taacgtccgc gcctttctcg gaaagctcaa tcatgcattc
    19501 gccaatcacg gcaatctttt tggacatagt cgtgaacctg atctgtaaaa aattagcgtt
    19561 agtttgcgct gtggtggttt gctggtcaac catattaaaa cagtgttcca ttattttttt
    19621 gagccagaac gtattcctga aagattccgt tgtggagtga ggaaatttac cccggccagg
    19681 cggtaaagtt ctgcccttac gcgccgataa tctttgtcga gtccgggcag catcactttt
    19741 aaacacagga catctttgat gataaggcag gttatccagc gaataagcaa ccctgaagca
    19801 agcatcgaga gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg
    19861 cagccgatct atcaaacatg cgggcggtta atggccgtgg agctattaac ggtggtcacg
    19921 catcccttga acccttcgca acgcctgccg ccggatcgct attttactga aatcaccgtc
    19981 agccatcgga tggaggttgt gaaagagcag attgatttgc tggcgcaaaa agccgacttc
    20041 tttgtagagc acggcctgct ggcatcggtc aatattgatg gccctacgct catcgccctg
    20101 cgtcagcaac caaaaatcct gcgccagatt gagcgtcttc cctggctgcg tttcgaactg
    20161 gtggagcata tccgtctgcc gaaagattca acctttgcct cgatgtgtga atttggcccg
    20221 ctgtggctgg atgattttgg taccgggatg gcaaatttct ctgcactaag tgaagtgcgt
    20281 tatgactaca tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc gccggaaggc
    20341 cgcacactct tttctcagct tttacatcta atgaatcgct attgtcgcgg ggtgattgtc
    20401 gagggcgtag aaacaccgga agagtggcgt gatgttcaga actcgcccgc attcgccgca
    20461 caaggctggt ttctttcacg cccggcaccg atggaaacgc tgaatacggc ggttctggcg
    20521 ctataagctg cctcattttc cgccgggctg gactatcttt aggactggca caggaagaat
    20581 atgaggaagc gaacgatgag caaggcaggc aaaataaccg ctgcgatttc aggggctttc
    20641 ttgttgttga ttgtcgtggc gatcattttg attgcaactt ttgactggaa tcgactcaaa
    20701 ccgaccatca accagaaagt ctctgcggag ttgaatcgtc cgttcgctat ccgtggcaat
    20761 ctgggcgtgg tgtgggagcg gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg
    20821 cctcatgtac atgcagaaga catcattctt ggtaatccac cggatattcc cgaagtcacg
    20881 atggtgcatt tgccgcgagt agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg
    20941 gtctggctgc cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg cctctctgaa
    21001 aagaacaata actggacgtt taatcttgcc aacgatgaca acaaagacgc gaatgcaaag
    21061 ccgtcggcat ggtcgtttcg gctggataat attcttttcg atcaagggcg gatcgccatt
    21121 gatgacaaag taagcaaagc ggatctggaa atttttgttg atccgttagg caagccgctg
    21181 ccattcagcg aagttactgg atcgaaaggt aaagcggata aagaaaaggt gggcgattac
    21241 atttttggcc tgaaggcgca gggacgttat aacggtgaac cgctcacggg tacgggaaaa
    21301 ataggcggta tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc
    21361 cgttcaggta atacccgtgt tgcttttgat ggcgtcgtga atgacccaat gaagatgggc
    21421 ggtgtcgatt tacggcttaa attttctggc gattcactgg gtgatctcta tgaactgacg
    21481 ggcgttctgc tgcccgatac cccgccgttt gaaacggatg gtcggctggt agcgaaaatc
    21541 gacactgaaa aatcgtcggt ctttgattat cgcggtttta atgggcgaat tggcgatagc
    21601 gatatccacg gttctctggt ctacaccacc ggaaagccac gaccaaaact ggaaggtgat
    21661 gtcgagtcgc ggcaattgcg gctggcggac ctgggaccgt tgattggcgt tgattccggg
    21721 aaaggggcag aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct
    21781 gcgggcaaag tgctgcctta tgaccgcttc gaaaccgata aatgggacgt tatggatgcc
    21841 gatgttcgct tcaaagggcg gcgcattgag catggcagta gcctgccgat tagcgatctt
    21901 tctactcata tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg
    21961 gcgggcggca gcattgcggc gaatattcat ctggaaggcg ataaaaagcc gatgcagggg
    22021 cgggcagata ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg
    22081 atgcagaaga cgctggggga aatgaacggt gacgcggaac tacgcggtag cggtaactcg
    22141 gtggcggcgc ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa tgacgggctg
    22201 gtgagccgca acctgatgga gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg
    22261 atatttggtg acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac
    22321 ggcgtggcgc gcccgcagat ttttgctttc gatactgaga acgcgttgat taatgttacc
    22381 ggcacggcaa gttttgcttc ggaacagctg gatttgacta ttgatccgga gagtaaaggg
    22441 attcggatta tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag
    22501 gctggggtga aagccggacc gctgattgcc cgtggtgctg ttgctgcggc actggcaacg
    22561 ctggtaacac cggcggcggc gttactggca ctgatctcac cttccgaagg ggaggctaat
    22621 cagtgtcgga cgatattgtc gcagatgaag aagtgatgat aattgccgga tgtctgacat
    22681 ccggcaatta aacagattta gatttttggc aaagtgtgct ttgttcatgc cggatgcggc
    22741 gtgaacgcct tatccggcct acaaatcctt gtgaattcaa tatattgtat ggaaattcag
    22801 tcctgataag cgtagcgcat caggcttttc acacttatta caacgactga tgtcgcgtct
    22861 catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga tataacccca
    22921 ccgcacctaa cccgtagtta gcctgcaacc aggctgcgat atacggcgca acggaagccc
    22981 cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact tctgtcggaa
    23041 acagctctgg taacagcgca cccatcggcc cgaaggtcag ccccatcaga cttaacccca
    23101 gcagcaggaa ggcaaaaacc agaatcgggt tgccagaacc gagcagcggg ttaaaggcga
    23161 acagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg cgaccaaagg
    23221 catcagccag taatccggcg actggcacca tcacgccaaa accaataact gccatcatca
    23281 gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg gcggtactga
    23341 aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc ataatgaacg
    23401 tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg atcttcacct
    23461 gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac acgcgaacat
    23521 acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag ccccagctca
    23581 taaactgctc atcggtcagt agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga
    23641 agccaatcgg tgcgcctagc tgtgggaagg agccatacag tgcacgtttg cgcggtgggg
    23701 cgttttcagt cgccagcagc gccgcaccgc cccattcacc gcctaagccc agaccctgac
    23761 caaatcgagc cagcgccagc agcagtgggg cgaaaatacc aatcgtggca tagcccggca
    23821 gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt
    23881 tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga
    23941 aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg ccctgcggga
    24001 agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag tcgaagaact
    24061 caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg atcggcgtgt
    24121 attcttgttc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct tattttttgg
    24181 ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg
    24241 aaatggtcaa aaaccaggca aaaaggggat aatgtttagg ccagtgattt ttcttctatg
    24301 aaatgcttca cataatttta taaatagtgg ataaaactgg tttcaggtta aataaaagtt
    24361 accaactgga tttatatgct ggggttatga gttggagtga aattgtttgc tcccaaatag
    24421 atggctgaag aggctctggg gaagagtttc aagaggttat gtgcgcatta tagccggatg
    24481 cgatgctggc gcatcttatc cggcctacgg ggagccaggc cgaataaaat cgtatgccgg
    24541 ataaggcgtt tacgccgcat ccggcctatt ttaaggctgc gttttccctg gcattcgcgg
    24601 gtcgtcttta tattcggcgg tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa
    24661 gaagtagaaa aatgccatca gccccagcac ggaaccaaat gctgcgccag agggggattt
    24721 catcagcgat ggcagggtgt aggtcatcac gattttaatc acttcaaaac caatagcagc
    24781 cagaaatgtt ccgcgaatca gggctttttt gcgcggacgg tggcgcggca accgccagaa
    24841 gatccagaag aaaagcagat agttggcgaa gatggaaatc gccaggccaa tcaatcgcca
    24901 cgtcggtttc agccactcaa tgctgttcag gtgcagggcg ctaataatca tttgctgcgc
    24961 cgaaccggca accgaggtga tcgaaagcgt cacaatcagc gcaatcaaca aaccaatcag
    25021 tgaaataaaa tcacgcagat attttaccca gaacttctcc tgatcttgcg gcgagcgttc
    25081 ccagacatcg cgcgactggg cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc
    25141 ggaataaagt gccaccgcca ggccgacaag ccctacagtc gtacgctgct gaacggcggt
    25201 gttgatggtg tttttcaacg tggcggctag tgtcggatcg ctgatgtttt gcagaatttt
    25261 gtcgaagata tcctgtagca acatcggatg ggaggccagc acaaagcccc ctgcggcaaa
    25321 cgacaccatc aaaatcggga tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa
    25381 ctggttaccc agccgatcgt taaagcgttc tgtcgcgcga ataaggtgcg caataaccgg
    25441 ttggcgttgg actttttcgg cagtggtggt gacggtttcc agcgcctggc taactttgcc
    25501 gcctttctcg ctattatcca actgcttaat cggctcgtgc tccagatcct gggtgggacg
    25561 tttgatctcg ttttcctgcg tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa
    25621 aagtatagtc ggtcagtcca ggtgctcttt cattacgcca gccagccact gcataaacag
    25681 gtttacacgc cgggaaagct cccgacgctg cgggtaaacc agcgaaaggg agagcggctc
    25741 ggcacggtag cctggcaaca cttcaataag ccgcccggca cgcagggctt cgcgcacggc
    25801 aatgcgcggg atctgaataa tccccagacc ggcaagaccg gcggcgagat agttttcgct
    25861 actgtttacc gtcaacatgc cgccagactt aaaccactgg acgccattaa cgctggcaac
    25921 ctcaaaacct aacggatgta cacccaggtg cggtgtataa cgcactattg cgtgtgaagt
    25981 cagatcatcg gggctttgcg gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt
    26041 gaccatggtt agtttgccga ggggacgggc gataacgcca tcttccggta acgcgccagt
    26101 gcgtatcacg caatcaaaac catcatgaag aatatctacc ggacggttat ggctactcag
    26161 ttccagctca attcccggat gctgatagag aaattccgac agacgcggca gtaacaggtt
    26221 tttcgcgatt cctgacggga tgtcgatgcg taatttaccg ctgatactgg tggcatcctg
    26281 ttgaaacaga ccgtccagtt cgctgagatt actcaacaca tcttttgccc gttgataata
    26341 ggtcatgcct tctggcgtga gtttgacccg tcgcgtagtg cgctggagaa gctgggtgcc
    26401 aagttgatgt tccagtgcct gtatctggcg cgaaacactt ccctttggca aagcaaagaa
    26461 atccgccgcg cgggaaaaac tttccagctc cgcgactttg atgaacaact gcattgcgtg
    26521 aattttatcc atttctttga ccgattgttg ttcacagcga aacagtaacg cgcatatcgc
    26581 attgtttatt gttttttcta acatctaatg ccgggtttca caatgttgtc atgacgaatg
    26641 tggcgatttc tcagcaggaa aagggcggaa tagctttatg agaaaggtga atccccttaa
    26701 acagagcgta tttttcagtg attcccttta aagtgaatag aggacgtatt ggctatttgt
    26761 gcttattttt gtctcattgc catttgttat tttggcttgt agtcgcggaa tttcattttt
    26821 aattgcatgc gtactaacca ccaatataag gaaatcctca aaattgtttt aaatagtgcc
    26881 ctcgacatgt aatttcaggc gtttagctgt aactatttat atcggtaact attttctacg
    26941 cgtcatctct gtagaatggc caccgccaaa tggcgaattc ttattttttc tcgcgggtaa
    27001 tacccttcca ctacttaagt ctttttaacg tattcaggtg gagattcagt tatgcaaata
    27061 gtcatgtttg acaggcagtc aatatttatt catggaataa aaatcagttt acagcagcgt
    27121 attccaggag tgagtattca gggggtcagt caggcagacg agttatggca aaagctggaa
    27181 agttaccctg aagccttagt tatgctcgat ggcgatcagg atggtgagtt ttgctactgg
    27241 ttgctgcaaa aaaccgtggt gcagtttcct gaggttcagg tgttaatcac ggcgacggat
    27301 tgcaataaac ggtggttaca ggaagttatc cattttaatg tgctggccat tgtgcctcgc
    27361 gattcaaccg tcgagacatt tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt
    27421 ctgcctggag actggcggac gacgccggaa aaggacatta aagatctcaa atcgttaagc
    27481 gcccgccagc gggagatttt aaccatgtta gcggcgggcg aatcaaataa ggagatcggc
    27541 agagcgctga atatcagtac cggaacggta aaagcgcatc tggaatccct gtatcgccgt
    27601 ctggaagtga aaaatcgcac ccaggcggca atgatgttaa atatctcctc ctg
//
LOCUS       DADLCQ010000051        27493 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000051 DADLCQ010000000
VERSION     DADLCQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.051"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(24..278)
                     /locus_tag="JQG95_001972"
     CDS             complement(24..278)
                     /locus_tag="JQG95_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY5888529.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JQG95_001973"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JQG95_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY5888530.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JQG95_001974"
     CDS             1228..2694
                     /locus_tag="JQG95_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY5888531.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JQG95_001975"
     CDS             2812..3798
                     /locus_tag="JQG95_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY5888532.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JQG95_001976"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JQG95_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY5888533.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JQG95_001977"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JQG95_001977"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY5888534.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JQG95_001978"
     CDS             5709..7265
                     /locus_tag="JQG95_001978"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY5888535.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILGSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JQG95_001979"
     CDS             7347..9161
                     /locus_tag="JQG95_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY5888536.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JQG95_001980"
     CDS             9162..10256
                     /locus_tag="JQG95_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY5888537.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSTFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JQG95_001981"
     CDS             10256..11281
                     /locus_tag="JQG95_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5888538.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JQG95_001982"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JQG95_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY5888539.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JQG95_001983"
     CDS             complement(12876..13220)
                     /locus_tag="JQG95_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888540.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQG95_001984"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JQG95_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY5888541.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFYIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQG95_001985"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JQG95_001985"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY5888542.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JQG95_001986"
     CDS             15616..17376
                     /locus_tag="JQG95_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY5888543.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JQG95_001987"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JQG95_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY5888544.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQG95_001988"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JQG95_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY5888545.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JQG95_001989"
     CDS             19113..19340
                     /locus_tag="JQG95_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY5888546.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JQG95_001990"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JQG95_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY5888547.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JQG95_001991"
     tRNA            21195..21271
                     /locus_tag="JQG95_001991"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            21478..22722
                     /locus_tag="JQG95_001992"
     CDS             21478..22722
                     /locus_tag="JQG95_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5888548.1"
                     /translation="MARKATPLTTTQIKAAKPAEKEYTLQDGGGLFLVIKPSGSKLWR
                     FNYYRPSNKKRTLISLGSLDEVSLADARKRRSEYRTLIAAGTDPQEYERQKREAEARR
                     QGNTFENVAALWYEMKKNQNLAHNTIKDIWRSLEKYVFPYIGNTPIDTLTARRFVEIL
                     TPIKARGNLETLKRVLQRINEVMDFAANSGMIDINTAANVRKTFPSPTKKHMPTIRPE
                     QLPQLMHDLSIASIERQTRLLIEWQLLTATRPAEASAARWEEINLEAATWTIPAGRMK
                     MRRDHVIPLCAQAMAVLEAMKPISARREHVFPSLKNPVQPMSSQTANAALRRMGYTGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYLEKRKVLMCWWGD
                     FVEAAATGTTIASGHRGLRGIK"
     gene            22997..23188
                     /locus_tag="JQG95_001993"
     CDS             22997..23188
                     /locus_tag="JQG95_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY5888549.1"
                     /translation="MRAILLRPEVKALLRIKSDSTLTKMVNKGGFPKGFRVGLRRIGW
                     YEDEVLAWLKERERESREA"
     gene            23415..23858
                     /locus_tag="JQG95_001994"
                     /pseudo
     CDS             23415..23858
                     /locus_tag="JQG95_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            23834..24022
                     /locus_tag="JQG95_001995"
     CDS             23834..24022
                     /locus_tag="JQG95_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY5888550.1"
                     /translation="MATIPALAHTQNAFIWRFIIVNPSDYRVVHVVASTEREARNYCP
                     SGCVAVFAARIRQGVSHA"
     gene            24015..24200
                     /locus_tag="JQG95_001996"
     CDS             24015..24200
                     /locus_tag="JQG95_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888551.1"
                     /translation="MLKRFQITARAVNHQGHVIDIRQNVNATSVNAAIAILKEELIGC
                     GFTQVMIRAVVEVSTVH"
     gene            24240..24539
                     /locus_tag="JQG95_001997"
     CDS             24240..24539
                     /locus_tag="JQG95_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY5888552.1"
                     /translation="MKVKNSGFTASGPVRPEIHTGDIFFDKYGWMVKVISVEERRITC
                     RREGYNYDCVTPVYQFRRDFSLMKAAPRSKPTSREKERGNIQKMKSMINTFRGKK"
     gene            24536..26671
                     /locus_tag="JQG95_001998"
     CDS             24536..26671
                     /locus_tag="JQG95_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAY5888553.1"
                     /translation="MKRAPNLKHQPADKMTEVIIFAGSDAWAHAKQWQEMDGRLAGDN
                     VPPVWLGDQQLAELENLNIIDDGRYCVRLYKAGHIKPSNINTIAQKLAAAGIRDANYY
                     PDGMHSQKRENWREYLERERQNIDDGLAIRLPTKENATGSHGDDELKPRVESRADGVF
                     WVTPKVDKQTGEIIRPETWLCSPLELLGTGTIGREHHRVMRWKKTANHEVITMAVPCG
                     GIGDRDGWRLLKDHGLNVTTNGKYRAILADWMQLSGNHEEWQLSTTTGWHFGAYIMPD
                     GSVIGESEKPILFTGKTAAVNGYSVAGTTEGWRDTVARLAGGNPSMMLGVAVSLSAPL
                     IGLVGADGFGVHLFEQSSAGKTTTQNIASSLWGEPDAQRLTWYGTALGIANEAEAHND
                     GLLPLDEIGQAGNAREVSTSAYTLFNGSGKLQGAKDGGNREIKHWRTVAISTGEMDVE
                     TFLKSEGIKVKAGQLVRLLNVPMEKSTQFHEYSTGKAHADALKDAWTANHGAAGREWI
                     KWLAAHQQKAKDTVRACRERWRNLIPESYGEQVHRVGERFAILEAALVLSGNVTGWDV
                     QACRDAIQHNFNAWVKEFGTGNKEHRQIIEQAEAFLAAYGMSRFAPVNYDPASLPISE
                     LYGYRESDGRYGESVLFYVLPEPFKSHVAKGFNKDAVARVLHEAGMLKKPASGEGWQI
                     RTPRLKHMKGARLRVYGLLLAQDHDDEGG"
     gene            26908..27117
                     /locus_tag="JQG95_001999"
     CDS             26908..27117
                     /locus_tag="JQG95_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY5888554.1"
                     /translation="MTAQISAYGRLVADPQLKTTSKGTPMALVSMAVPIPCNQADDGQ
                     ATMWLSVLAFGRQADALAKHCKYPA"
     gene            27179..27493
                     /locus_tag="JQG95_002000"
                     /pseudo
     CDS             27179..27493
                     /locus_tag="JQG95_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6992 a         6833 c         6945 g         6723 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactgggta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcacattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg taaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctactg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaatcaacta tagctaatca tcgtttttat
    21301 gaaaaaagcc cgctaattca ttgaatagcg ggttttttta ttgtttttag tcatcgttat
    21361 tcgacggtaa cagtggtttt cagccgtttt ttttagttat atgtttagtt atacgggctg
    21421 gcgcatagct aaatccgtat aactagaacc tgtttttccc cgtggattca taaacgtatg
    21481 gcaagaaagg caaccccact caccaccaca cagatcaaag cagctaaacc agcagaaaaa
    21541 gaatacacct tacaggatgg cggcgggctt tttctcgtta ttaagccgtc tggttcgaaa
    21601 ctatggcgat tcaactacta tcgaccttcg aacaaaaaac gaacactcat tagtttggga
    21661 tcgcttgatg aagtctccct tgctgatgcc agaaaacgcc gtagcgagta caggacgtta
    21721 attgcggcag gaaccgaccc gcaggaatac gaacggcaaa aacgcgaagc agaggctcga
    21781 cgacagggca acacgttcga aaatgtggcc gcattgtggt acgaaatgaa aaaaaaccag
    21841 aatctcgccc acaatacgat caaggacatc tggcgctcgc tagagaaata tgtattcccg
    21901 tatatcggaa acacaccgat agatactctc accgctcgcc gctttgttga gatactcacg
    21961 cccatcaagg cacgtggcaa cctggaaaca ctgaaacgcg ttttacagcg catcaatgaa
    22021 gtgatggatt ttgctgccaa cagtggaatg attgacatca acaccgccgc gaacgttcgc
    22081 aagacattcc cttctcccac caaaaagcac atgccaacca tccggccgga acaactaccg
    22141 cagctaatgc acgatttatc gatcgccagc atagaacggc aaaccagatt gctgattgag
    22201 tggcagttgt taaccgcaac ccgcccagcc gaagcctctg ccgcacggtg ggaagaaatc
    22261 aaccttgagg cggcaacctg gacgatacca gccggacgca tgaagatgcg ccgtgatcat
    22321 gtgatccccc tttgcgctca ggcgatggca gtgcttgaag ccatgaagcc gattagcgca
    22381 cgaagggagc atgttttccc aagtcttaaa aatccagtgc aaccaatgag cagccagaca
    22441 gcaaacgcag cattacggcg aatgggttac actggtgtgc tggtatctca cggactacgc
    22501 gccatattca gcacagcggc gaacgaagaa ggatttgagc cggacgtaat cgaggccgca
    22561 ctcgcacacg tggacacgaa cgaagttaga cgggcataca accgaagcaa ctacctggaa
    22621 aaacgtaaag tgttaatgtg ctggtggggt gattttgtgg aagcagccgc aaccggaaca
    22681 accatcgcca gcgggcacag aggattacgc ggaataaaat aaaatattaa aaaaattatt
    22741 agctcctaat tggtatcagt taggagccta tttggagcta atttaaattt catattttta
    22801 tgttagaaaa ttaattttaa atttctaacg ccattatcta aagttagaag ccccattatt
    22861 taaagttaga agaaaaataa ttattttttg ctaaatggct cttgacttat ttttttatga
    22921 ttttgtatgc ttattacatc acttaccgtt attttcattc aaaaagcgat attttgctgt
    22981 ccagaggaaa aataagatga gggctattct tttacgacca gaggtaaagg ctttgctacg
    23041 cattaaatct gattcgaccc taacgaagat ggttaacaaa ggcgggttcc ccaaaggatt
    23101 ccgtgtcggg ctgcgtcgca ttggctggta tgaagatgag gtactcgcct ggttgaaaga
    23161 acgtgagagg gaaagtcgtg aagcgtaagt gtgattaacg ttcgcctgat tactatcaag
    23221 taataagaca attaagagag aacattatga atactggcaa aaatgcccaa gcaggggcag
    23281 gttttcgttc aaccataaaa aatatggctg atgttattat tcagttcaaa ccttacaaaa
    23341 gaatcaattg aactaatcgc acttccgttt taaacggaaa cgtacaatcg catagcttaa
    23401 acgacaaaag aaatatgctt tttacaaata aaataaaggc gaccgcagag ggccgccaga
    23461 ggacatgtat taaacttgaa cgcatcgcca acaatgccat atttacagct ggtgggcaat
    23521 gtgatttgtg tggtgggcac accttcaaca catccgacca catgggccgg aaaacgggat
    23581 atactcgccg aatcccttat aaaacgggga tcgggattac caccccgctg aagcaaaagg
    23641 cgacacatga cgcgccagcg tcttttttgt tgtctgtaca cacgcacacc ttatcaatgg
    23701 tgggctgtat ggggctgact tcggtcaggc tggtctcctt ttgcgccagt tgtggtaacc
    23761 ctgtacagtc caccgccagc gagcttacca cctcctgcgg tgggtatatc ccaacgcaaa
    23821 aggaggctgc caaatggcta ctatccctgc cctcgcacat actcagaacg cctttatctg
    23881 gcgctttatc atcgttaatc cgagtgatta ccgcgtcgtt cacgtcgtgg cgtcaaccga
    23941 acgagaagcg cgtaactatt gcccgtccgg ttgtgttgcc gtattcgctg cccgtattcg
    24001 tcagggggta agtcatgctt aagcgattcc agataacagc acgcgctgta aatcatcaag
    24061 gccacgttat tgatattcgg caaaacgtga acgccaccag cgttaatgca gctatagcaa
    24121 tattgaaaga agagttaatc ggctgtgggt tcacgcaggt gatgatccgt gccgtagttg
    24181 aagtgtcgac tgttcattaa tctcattaat acagctttaa caaaaacgta attcgacaaa
    24241 tgaaagtaaa aaattctggc tttactgcca gcggccccgt tcggcctgaa atccacaccg
    24301 gcgatatttt cttcgataaa tacggctgga tggtaaaggt gataagcgtg gaggaacggc
    24361 gcatcacctg ccgtcgtgaa ggatataact acgactgtgt aacgcctgta tatcagttcc
    24421 ggcgtgattt ctccctgatg aaggccgcgc cccgcagtaa acctactagc agggaaaaag
    24481 agcggggaaa tatccagaaa atgaaaagca tgattaacac attcaggggc aagaaatgaa
    24541 acgtgcaccg aatttaaagc atcagccagc cgacaaaatg acggaggtta tcatttttgc
    24601 gggtagtgat gcctgggcac atgcgaagca gtggcaggag atggacgggc gacttgctgg
    24661 cgataacgtg cctcctgtct ggcttggaga tcagcaactg gcagaactgg aaaatctgaa
    24721 catcattgac gacgggcgct attgtgtccg gctgtacaag gcagggcaca tcaagccatc
    24781 aaacatcaat accattgcgc aaaagctggc ggcggcaggc atacgggatg cgaattatta
    24841 ccccgacggg atgcacagcc agaagcggga gaactggcgc gaatatctgg aacgtgagcg
    24901 ccaaaacatc gatgacgggc tggctatccg gcttccgaca aaagaaaacg ccaccggaag
    24961 tcatggcgat gatgaattaa agccccgcgt tgaaagccgc gcagatggtg ttttctgggt
    25021 aacacccaaa gtggacaagc agacaggcga gataatcaga cctgaaacgt ggttatgttc
    25081 cccgcttgaa ctactgggaa cggggacgat cggcagggag catcaccgcg tgatgcgctg
    25141 gaaaaaaacg gcaaatcatg aagtcatcac gatggcggtt ccgtgcggtg gcattggcga
    25201 ccgtgacggc tggcggttgt tgaaagacca cggccttaac gtgacaacca acggcaaata
    25261 cagagcaata ctggctgact ggatgcagtt aagcggaaac catgaggaat ggcagttaag
    25321 cacaacaacg gggtggcatt ttggcgcgta catcatgccg gacggttcag tcattggtga
    25381 gtctgaaaaa ccgatcctgt ttaccggaaa aacggctgcc gttaatggtt attccgtagc
    25441 aggaacgacg gagggctggc gcgatacagt ggcgcggttg gctggtggca atccgtccat
    25501 gatgctgggg gtggcggtat cgttatcagc gccattaatc gggctggtgg gcgctgacgg
    25561 tttcggtgtg catcttttcg aacaatcatc ggcaggtaag accaccacgc agaacatcgc
    25621 atccagtttg tggggagagc cggacgcaca acggctgacc tggtacggaa cggcgttagg
    25681 tatcgccaac gaggccgaag cacacaatga cgggctgtta cccctggatg aaataggcca
    25741 agccggaaac gcacgggagg tgtccacgtc ggcttatacg ctgtttaacg gttcgggaaa
    25801 attacagggg gcgaaggacg gcggcaacag ggagataaaa cactggcgaa cagtggcaat
    25861 cagtaccgga gaaatggacg ttgaaacatt cctcaaaagt gaggggataa aagtcaaagc
    25921 ggggcagctt gtccggttgc ttaacgtgcc gatggagaag tccacgcagt ttcacgaata
    25981 cagcacagga aaggcgcacg cagacgcgtt aaaggatgcc tggacagcaa atcacggggc
    26041 ggcaggtcgt gagtggatta aatggctagc agcccaccag cagaaggcta aggacacggt
    26101 aagggcatgc cgtgaacggt ggcgcaacct gataccggag agctacggcg aacaggttca
    26161 ccgtgtaggg gaacgattcg ccatactgga ggccgcgctt gtgctgtcag gcaatgttac
    26221 gggttgggat gttcaggcgt gccgtgatgc cattcagcat aactttaatg cctgggtgaa
    26281 ggagttcggc acgggtaaca aggaacacag gcagattatt gaacaggcag aggcgtttct
    26341 tgctgcctac ggcatgagca ggtttgcgcc tgtcaattat gaccctgcaa gcctgccgat
    26401 ttcagaactg tacggctaca gggaaagcga cgggcgttac ggtgagtctg ttttgtttta
    26461 cgtcctgccg gagccattta aatcgcatgt ggcgaaggga tttaacaagg atgccgtagc
    26521 aagagtcctt cacgaagctg gaatgctgaa aaagccagcc agcggggaag gatggcaaat
    26581 cagaacacca cgtcttaaac acatgaaggg ggcacgacta agggtgtacg gccttttact
    26641 tgcacaggat catgatgatg aaggaggtta atctctcgcg tgtaggttta aaaacacttg
    26701 tcccacctgt cccaacctgt gggaaacaca cataacacac tgaattatat tgggtaacat
    26761 cagaaaaagt gggacaagtg tgggacaagt taaggtgatt ttgggacaag ttaggagcat
    26821 tttgggacaa gtcagcaggc gttatttttt gcctggctgg tggacattaa aaatccaacc
    26881 aacatttaaa accggagatc agacaatatg acggcacaaa tatcagctta cgggcggctg
    26941 gtagctgacc cgcagttaaa gaccaccagc aagggaacac caatggcgct ggtgagtatg
    27001 gctgttccca ttccgtgcaa tcaggcagat gacggacagg cgacgatgtg gttatccgtc
    27061 ctggcatttg gcagacaggc cgacgcactg gcaaaacact gtaagtatcc cgcataatcg
    27121 tgccattcac atttagagat cctccggcat actcaatctg ccaattaagg agatcgctat
    27181 gcgtaaagcc cgttttactg agcatcagat catcgccgtg attaagtcgg ttgaagccgg
    27241 acgaactgtt aaagatgtct gccgggaggc cggtatctct gaagccacct actacaactg
    27301 gaagtccaga tacggtggca tggaggcttc tgatattaaa aagattaaag agcttgagga
    27361 cgagaaccgc cgtctcaaac agatgtttgc cgacctgagc ctggagaacc gggcgctgaa
    27421 ggacgttatc gaaaaaaagc tttaaaacca gcctgtaagc gtgagctggt cactcatctg
    27481 ataacgacat tcg
//
LOCUS       DADLCQ010000052        26076 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000052 DADLCQ010000000
VERSION     DADLCQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.052"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQG95_002001"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="JQG95_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY5888555.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="JQG95_002002"
     CDS             complement(2138..2584)
                     /locus_tag="JQG95_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY5888556.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="JQG95_002003"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="JQG95_002003"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY5888557.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQG95_002004"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="JQG95_002004"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY5888558.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="JQG95_002005"
     CDS             complement(5347..6009)
                     /locus_tag="JQG95_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY5888559.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="JQG95_002006"
     CDS             6182..6595
                     /locus_tag="JQG95_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY5888560.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQG95_002007"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="JQG95_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY5888561.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQG95_002008"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="JQG95_002008"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY5888562.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="JQG95_002009"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="JQG95_002009"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY5888563.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQG95_002010"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="JQG95_002010"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY5888564.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQG95_002011"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="JQG95_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY5888565.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="JQG95_002012"
     tRNA            complement(9861..9948)
                     /locus_tag="JQG95_002012"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="JQG95_002013"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="JQG95_002013"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY5888566.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="JQG95_002014"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="JQG95_002014"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY5888567.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="JQG95_002015"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="JQG95_002015"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY5888568.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="JQG95_002016"
     CDS             14005..14592
                     /locus_tag="JQG95_002016"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY5888569.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="JQG95_002017"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="JQG95_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY5888570.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="JQG95_002018"
     CDS             14984..15841
                     /locus_tag="JQG95_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY5888571.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPM"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="JQG95_002019"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="JQG95_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY5888572.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="JQG95_002020"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="JQG95_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY5888573.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="JQG95_002021"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="JQG95_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY5888574.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /locus_tag="JQG95_002022"
     CDS             18851..20149
                     /locus_tag="JQG95_002022"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY5888575.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22444)
                     /gene="etk"
                     /locus_tag="JQG95_002023"
     CDS             complement(20264..22444)
                     /gene="etk"
                     /locus_tag="JQG95_002023"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY5888576.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKLGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSNAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGTILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(22464..22910)
                     /gene="etp"
                     /locus_tag="JQG95_002024"
                     /gene_synonym="yccY"
     CDS             complement(22464..22910)
                     /gene="etp"
                     /locus_tag="JQG95_002024"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY5888577.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(22898..24037)
                     /locus_tag="JQG95_002025"
     CDS             complement(22898..24037)
                     /locus_tag="JQG95_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY5888578.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24083..25282)
                     /locus_tag="JQG95_002026"
     CDS             complement(24083..25282)
                     /locus_tag="JQG95_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY5888579.1"
                     /translation="MIVKEILNIQLIHLKYNAGFDAPEIQLRDKTLYMSGQQYKYRDS
                     REAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAGTAPGKSEQL
                     QQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGLMSSARYWFT
                     DHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLNNLQANYFAD
                     LGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWDNMMRFTDYS
                     TPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVGVWAAISNVS
                     KDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRKYQLDPMTAE
                     REVPVGQ"
     gene            complement(25322..26017)
                     /locus_tag="JQG95_002027"
     CDS             complement(25322..26017)
                     /locus_tag="JQG95_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5888580.1"
                     /translation="MLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHVATNPVTATRT
                     AKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCRMKWSDINDNH
                     LHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKHHDPLSPKTVS
                     KYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHKSDSMAARYRD
                     SRGREWDKIEIDK"
BASE COUNT         6217 a         6648 c         6906 g         6305 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggccggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgatgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccaggaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaaa
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgctact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgatgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgtgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gcctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga gtaagcgaca gcgatcagcg
    22321 tgaataacgc ggtcacgctg ataataaact tacggtgatc ccataactcg ccgaccagac
    22381 gaagcagatc gatctcattt tcctgagtgc tgcctggtgg cgtattcata tttttagttg
    22441 tcatcgtatt ttataaactc aaattaccgg ctgaggcgct tcgcccattc ctgactggcg
    22501 cgctccaaca taccgtagac atgttcaaat gcgtcctgac ttttacgata gggatccggg
    22561 atctctttct gttccagcca ttgcccaaac agcattgtct tcccgcggac ctcgggagca
    22621 attgcggtaa cctgagcaat atgttccgac tccattgcca gaatcagatc gtaatttcgt
    22681 gccatctcag cagtgagctt acgtccggca tgtccttcaa gagatacgcc atgattagcg
    22741 gcaacatctg ccgctgtcgc atctgcaggg tgttttacca gaccatgaac acctgccgat
    22801 ttgactttta cgcccggcag acgtttacgc agcaagcgct cgccaatggg cgaacggcaa
    22861 atgttgccgg tacaaaccac taggattgag ttaaatttta gttgggccat gtttgaatcc
    22921 accgtgaagt ctctgtcagg tcatgcaccc cggaaatcgt cggtaccagc agagaaatca
    22981 cacggttcca acgagccagc ggcgcggtag tgacataaac aatgtcgtaa ggttcaagct
    23041 ggaattccgt tccgagtatc attgccgatg cgtccttcgc attcagctga taaatattgg
    23101 cgattttgcc attctgcttg ttttgcgttg cacgaataac aaaaatcccc gtcgcatcag
    23161 cgacatcctg attcataccc tcagcgttac ccagcgcttc tgccagggtc ataccgctgc
    23221 ggtccatttt cagcgtgctc tgtttaccca cttcacccat cacgaagact ttcagatcat
    23281 cgttgcgcgg aataaataaa atatcgcccg ggtgcagcag tttgttctgg cgcaagtcgc
    23341 cacgctgcat cagcgcatag aggttcactt tggtctttac gccattctgg gtgagcacca
    23401 cgttgcgcca gtctgcatct gcggtcagac cgcccgcggc gttaatcgca tccatgatgg
    23461 tcagaggaat attggtaatc ggctgctgac ccgatttgct gacttcgccg gtgacgtacg
    23521 ctttttgcga tcggaacgcc gcgacgctga catcgacttg cgggctttca atgactgaat
    23581 cgagacgcgc tgtaatctcg ttgcgtacct gggtaagtgt tttcccggcc actttcaggc
    23641 gaccaatata ggggtagaaa atcgcaccat cggcgttaac ccagttgccg gtatcactgg
    23701 cgctacggta ttgccctgcg ggggtggtta gttccgggtg atcccagacc gtaaccatca
    23761 acacatcacc aatcccgata cgatattcat agtttgcgat ttgctgctcc agctccgggt
    23821 tagcctgcga cataatcggt ttggcgcgca gctgatcaat caatcctggc gtgacgggat
    23881 agatattgac cattttgtcc aggtcatact gattgtcagg tagttcaatg acatccttgt
    23941 tactggtcga taaattctga ccaggaacaa gggtacatgc tgtcaaagac agaacagcca
    24001 atgtcagtac cgaaaactta aaaatgttct ttttcattgg tttatattta tcagtcaatg
    24061 taatgttatg aattacgtca ggttattgtc caaccggtac ttctcgctct gccgtcattg
    24121 gatcgagctg gtatttgcgt gacagcattt gtccaccatc acgcgtcaat ggtgtccacg
    24181 aaaccaccgc gcggttgcgg ttaggtccaa tggtcatcaa gtcgaacgga atcgagatat
    24241 aaaaaccttt actaaagccg ccttcgccgt agtcatcttt agatacgtta ctgattgccg
    24301 cccatacccc taccgccacg ccgctgtcaa agcgtttggc gacgtcgatc gttgcgcctt
    24361 tatcttttgc cagatattgc ccaacgctaa gtttcatcag tacgccattg agcgtcggcg
    24421 ggttccagta agccgtcacg aaacctgtgg gcgtggaata atcggtgaag cgcatcatgt
    24481 tgtcccagtc acgctgcttc acgtagttaa cgtccacacc cagcgcccag ctggcatcta
    24541 gcgggcgata aagaagctcg gaaccgacac cggcatacat cgtttccaga taaccgccat
    24601 acacctggcc atagaaacca ttgcctaagt cggcaaagta attcgcttgc aagttgttga
    24661 gataaacgtc attgcgaacg taatcacgga tatgcgtgcg cacgcggggc agggtagagt
    24721 ccgcgggtaa cagtgaagac ttaaacttgt cgtagttgtt gtaaatattg gtgaaaatac
    24781 cgtcatcaag cagcaggtgg tcggtaaacc agtagcgggc gctggacatc agccccagct
    24841 ggaacatata gaaatcttcc gggccgccca gcgactgtga aagtgttggg ttgaaagagt
    24901 agctaaagcg atcttcacga atacggtagc cccgaccaaa ggcagaaaga tcttctgctt
    24961 caacacgttg ttgttgcagt tgctctgatt taccaggcgc tgtgccggcc agctgtttgc
    25021 gcaggctggc tacgtcggtt tcggtagtca ccatcgccat atgctcgcgc ttttgcgtca
    25081 cgctaatctt ctcaacgcct tgcggcaggt tattcaccag aatccggttg gcacgatcga
    25141 ccgcttcgcg agagtcacgg tatttgtatt gctgaccaga catatacagc gtcttatcgc
    25201 gtagctgaat ttctggcgcg tcaaagcccg cgttatactt caggtgaata agttgtatat
    25261 ttaaaatctc tttgactatc aatgaattag tataagaagg tcattattag tcaaaataaa
    25321 atcatttgtc gatttcaatt ttgtcccatt cccgtccacg gctgtcccta taccgcgccg
    25381 ccattgaatc tgatttatgt ccgagaagac gttgagcaaa cttatcgcca atctgattac
    25441 ggtatagcct cgccgacagg ctacgcagtt catggaatgt tggcgggtct ccatcaaatg
    25501 agagtcctga tgcatttctc gcctttgtaa aatacttcga tactgttttc ggggaaagcg
    25561 gatcgtgatg ctttgatgcg attatagttt cactgctgct ggcctccctg catttctgta
    25621 gtgtatcagc caatgagata ttgagcgcgt caatcgttag cgtcagcgga atggcgagct
    25681 tagcccctgt tttgctctgt tcaatgtgaa gatggttgtc gtttatgtct gaccatttca
    25741 ttctgcacaa atcgccgact ctctgccctg taacgacagc caaatccatt gctagcctca
    25801 gccagattgg gagaggttcg gctgcatggt aaatcgcgac atactcatta gctgtcagcc
    25861 ttgagcgcct tacttctgac tttgctgtgc gggttgctgt taccggattc gttgctacat
    25921 gcccctcggc tattgcctca cgaaaaacgt caacaagggt tgacctgatt aattttgcgg
    25981 aagccgcttt accttctgct acgtaggtgt ttagcattgc tgccacctct ttcgttgata
    26041 tgtcagcgag cggtttgtcc ggcaattttc ttcgga
//
LOCUS       DADLCQ010000053        24600 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000053 DADLCQ010000000
VERSION     DADLCQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.053"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..374)
                     /locus_tag="JQG95_002028"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="JQG95_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..2826
                     /gene="thrS"
                     /locus_tag="JQG95_002029"
     CDS             898..2826
                     /gene="thrS"
                     /locus_tag="JQG95_002029"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY5888581.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2830..3372
                     /gene="infC"
                     /locus_tag="JQG95_002030"
     CDS             2830..3372
                     /gene="infC"
                     /locus_tag="JQG95_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY5888582.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3469..3666
                     /gene="rpmI"
                     /locus_tag="JQG95_002031"
     CDS             3469..3666
                     /gene="rpmI"
                     /locus_tag="JQG95_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY5888583.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3719..4075
                     /gene="rplT"
                     /locus_tag="JQG95_002032"
     CDS             3719..4075
                     /gene="rplT"
                     /locus_tag="JQG95_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY5888584.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     gene            4198..4242
                     /locus_tag="JQG95_002033"
     CDS             4198..4242
                     /locus_tag="JQG95_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY5888585.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4525..5508
                     /gene="pheS"
                     /locus_tag="JQG95_002034"
     CDS             4525..5508
                     /gene="pheS"
                     /locus_tag="JQG95_002034"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY5888586.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5523..7910
                     /gene="pheT"
                     /locus_tag="JQG95_002035"
     CDS             5523..7910
                     /gene="pheT"
                     /locus_tag="JQG95_002035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY5888587.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQHRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7915..8214
                     /gene="ihfA"
                     /locus_tag="JQG95_002036"
     CDS             7915..8214
                     /gene="ihfA"
                     /locus_tag="JQG95_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY5888588.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8315..9295
                     /gene="btuC"
                     /locus_tag="JQG95_002037"
     CDS             8315..9295
                     /gene="btuC"
                     /locus_tag="JQG95_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY5888589.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9358..9909
                     /gene="btuE"
                     /locus_tag="JQG95_002038"
     CDS             9358..9909
                     /gene="btuE"
                     /locus_tag="JQG95_002038"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY5888590.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9909..10658
                     /gene="btuD"
                     /locus_tag="JQG95_002039"
     CDS             9909..10658
                     /gene="btuD"
                     /locus_tag="JQG95_002039"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY5888591.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     QMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10736..11161
                     /locus_tag="JQG95_002040"
     CDS             10736..11161
                     /locus_tag="JQG95_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY5888592.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRDRFDLHLPRETKQQASIGTQIDKDELLPGDLVFFKTGSGQNGLH
                     VGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11408..12121
                     /gene="ydiV"
                     /locus_tag="JQG95_002041"
     CDS             11408..12121
                     /gene="ydiV"
                     /locus_tag="JQG95_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY5888593.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12185..13621
                     /locus_tag="JQG95_002042"
     CDS             12185..13621
                     /locus_tag="JQG95_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY5888594.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="JQG95_002043"
     CDS             complement(13625..13816)
                     /gene="hemP"
                     /locus_tag="JQG95_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY5888595.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="JQG95_002044"
     CDS             complement(13948..14994)
                     /gene="aroH"
                     /locus_tag="JQG95_002044"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY5888596.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAIEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="JQG95_002045"
     CDS             complement(15151..15984)
                     /gene="ppsR"
                     /locus_tag="JQG95_002045"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY5888597.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16316..18694
                     /gene="ppsA"
                     /locus_tag="JQG95_002046"
     CDS             16316..18694
                     /gene="ppsA"
                     /locus_tag="JQG95_002046"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY5888598.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18751..20451)
                     /gene="fadK"
                     /locus_tag="JQG95_002047"
     CDS             complement(18751..20451)
                     /gene="fadK"
                     /locus_tag="JQG95_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAY5888599.1"
                     /translation="MHPTGPQLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKIGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRSIKLLSVYGSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEMAAFFSRKRVAKYKYPEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE
                     "
     gene            complement(20454..20747)
                     /locus_tag="JQG95_002048"
     CDS             complement(20454..20747)
                     /locus_tag="JQG95_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY5888600.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20744..22033)
                     /locus_tag="JQG95_002049"
     CDS             complement(20744..22033)
                     /locus_tag="JQG95_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5888601.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22089..23027)
                     /locus_tag="JQG95_002050"
                     /pseudo
     CDS             complement(22089..23027)
                     /locus_tag="JQG95_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(23047..23811)
                     /locus_tag="JQG95_002051"
     CDS             complement(23047..23811)
                     /locus_tag="JQG95_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY5888602.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24127..>24600
                     /locus_tag="JQG95_002052"
     CDS             24127..>24600
                     /locus_tag="JQG95_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY5888603.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLA"
BASE COUNT         6094 a         5940 c         6465 g         6101 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag ggcagtggaa taacaatttt gtgatatttt
      181 cgacagtttg cggtaaattc ctgagtaaca cagtatgaca gcatcgaact taagtcagag
      241 gtatcaagta gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt
      301 ggtatgtcgc tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata
      361 tcgttttgac tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt
      421 aattattatc gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt
      481 atcattgtga tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa
      541 atcacgcaac gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac
      601 ctttcagacg caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact
      661 acaaacgaat tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag
      721 tgttaacatc gttcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg
      781 attgcgaacc aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca
      841 aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg
      901 cctgttataa ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg
      961 gatgttgcgc tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat
     1021 ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc
     1081 gccaaagacg aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcat
     1141 gcgattaaac aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt
     1201 ttttattacg acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag
     1261 aagcggatgc atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg
     1321 cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct acaaagtttc cattcttgac
     1381 gaaaacatcg cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg
     1441 tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg
     1501 gcaggggctt actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg
     1561 gcgtgggcag acaaaaaagc acttaacgct tacctgcagc gcctggaaga agccgcgaaa
     1621 cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg
     1681 ggtatggtat tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt
     1741 cgttctaaac tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt
     1801 gtcctgtggg aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacatct
     1861 tctgagaacc gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc
     1921 aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc
     1981 caccgtaacg agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag
     2041 gatgacgcgc atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc
     2101 cgtttagtct atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc
     2161 actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg
     2221 gcggttgcgc tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc
     2281 tacggtccga aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt
     2341 acagtacagc tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac
     2401 aacgagcgta aggtaccggt aatgattcac cgcgcaattc tggggtcgat ggagcgtttc
     2461 atcggtatcc tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag
     2521 gttgttatca tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa
     2581 ctatcaaatg cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa
     2641 atccgcgagc acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg
     2701 gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta
     2761 aatgaagtga tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag
     2821 gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc
     2881 gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg
     2941 agtctgagag aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc
     3001 cctaacgccg agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag
     3061 agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa
     3121 ttccgtccta gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt
     3181 ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac
     3241 cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcggta
     3301 gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag
     3361 aagaaacagt aaggccttca agtagcacta tctgtggagc ctttgggctt cacaggcatt
     3421 gttcgccttt gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt
     3481 aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac
     3541 aagcacgcta acctgcgtca cattctgact aaaaaagcga ccaaacgtaa acgtcacctg
     3601 cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac
     3661 gcataagccg ttaacgtttt taacttttta attagaatat agatacagga gagcacatat
     3721 ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc
     3781 taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa
     3841 agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg
     3901 gattgcgcgt atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa
     3961 tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt
     4021 cgacaaagta gcgttcacca ctctggttga aaaagcgaaa gcagctctgg cataagccag
     4081 ttgaaagagg gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta
     4141 tcgccgtagc cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg
     4201 aatgctgcta ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg
     4261 tgagaagaga aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc
     4321 gcgttttgaa aaataaaaag ccagtctatc aggcgtaatt gtcgcagcca gtctggacac
     4381 ggccagtgcg gagaaaccgg agcgtactta agtacgtgag gatttcgagc actgcccggg
     4441 accaaaatgg caagtaaaat agcctgatgg gataggctct aagtccaacg aaccagtgtc
     4501 accactgaca caatgaggaa aaccatgtca catctcgcag aactggttgc cagtgcgaag
     4561 gcggccatta gccaggcgtc agatgttgcc gcgttagata atgtgcgcgt cgaatatttg
     4621 ggtaaaaaag ggcacttaac ccttcagatg acgaccctgc gtgagctgcc gccagaagag
     4681 cgtccggcag ctggtgcggt tatcaacgaa gcgaaagagc aggttcagca ggcgctgaat
     4741 gcgcgtaaag cggaactgga aagcgctgca ctgaatgcgc gtctggcggc ggaaacgatt
     4801 gatgtctctc tgccaggtcg tcgcattgaa aacggcggtc tgcatccggt tacccgtacc
     4861 atcgaccgta tcgaaagttt cttcggtgag cttggcttta ccgtggcaac cgggccggaa
     4921 atcgaagacg attatcataa cttcgatgct ctgaacattc ctggtcacca cccggcgcgc
     4981 gctgaccacg acactttctg gtttgacgct acccgcctgc tgcgtaccca gacctctggc
     5041 gtacagatcc gcaccatgaa agcccagcag ccaccgattc gtatcatcgc gcctggccgt
     5101 gtttatcgta acgactacga ccagactcac acgccgatgt tccatcagat ggaaggtctg
     5161 attgttgata ccaacatcag ctttaccaac ctgaaaggca cgctgcacga cttcctgcgt
     5221 aacttctttg aggaagattt gcagattcgc ttccgtcctt cctacttccc gtttaccgag
     5281 ccttctgcag aagttgacgt catgggtaaa aacggtaaat ggctggaagt gctgggctgc
     5341 gggatggtgc atccgaacgt gctgcgtaac gttggcatcg acccggaagt ttactctggt
     5401 ttcgccttcg ggatggggat ggagcgtctg actatgttgc gttacggcgt caccgacctg
     5461 cgttcattct tcgaaaacga tctgcgtttc ctcaaacagt ttaaataagg caggaataga
     5521 ttatgaaatt cagtgaactg tggttacgcg aatgggtgaa cccggcgatt gatagcgata
     5581 cgctggcgaa tcaaatcact atggcgggcc tggaagttga cggtgtagaa ccggttgccg
     5641 gtagcttcca cggcgtggtc gttggtgaag tggttgagtg tgcgcagcat ccgaacgctg
     5701 acaaactgcg tgtgacaaaa gtgaatgtcg gcggcgatcg cctgctggac atcgtctgcg
     5761 gtgcgccaaa ctgccgtcag ggcctgcgtg tggcggtagc taccattggt gctgttctgc
     5821 cgggtgattt caaaattaaa gcggcgaaac tgcgtggcga accgtctgaa gggatgctgt
     5881 gctccttttc tgagctggga atttctgacg atcacagtgg cattatcgaa ctgcctgcgg
     5941 atgcgccgat tggcaccgac atccgcgaat acctgaaact cgatgataac accatcgaaa
     6001 tcagcgtgac accaaaccgt gccgactgct taggtatcat tggtgttgcg cgtgacgttg
     6061 ccgtgctgaa tcagctgccg ctggttgaac cggaaatcgt tccggttggt gcgaccatcg
     6121 acgacacgtt gccgattaca gtcgaagcgc cggaagcctg cccgcgctat cttggccgtg
     6181 tggtaaaagg cattaacgtt aaagcgccaa ctccgctgtg gatgaaagaa aaactgcgtc
     6241 gttgcgggat ccgttctatc gatgcagttg ttgacgtcac caactatgta ctgctcgaac
     6301 tgggccagcc gatgcacgct ttcgataaag atcgcattga aggcggcatt gtggtgcgga
     6361 tggcaaaaga gggcgaaacg ctggtgctgc ttgatggcac tgaagcgaag ctgaatgctg
     6421 acactctggt catcgccgac cacaacaagg cgctggcgat gggcggcatc tttggtggcg
     6481 aacactctgg cgtgaatgac gaaacgcaaa acgtcttgct ggaatgcgct ttcttcagcc
     6541 cgctgtctat caccggtcgt gctcgtcgtc atggcctgca tactgatgcg tctcaccgtt
     6601 atgagcgtgg cgttgatccg gtactgcagc acaaagcgat ggaacgtgcg acccgtctgc
     6661 tgatcgacat ctgcggtggt gaggctggcc cggtaattga tatcaccaac gaagcaacgc
     6721 tgccgaagcg tgcaaccatc actctacgtc gtagcaaact ggatcgcctg ataggccatc
     6781 atattgctga tgagcaggta actgacattc tgcgtcgtct cggctgcgaa gtgaccgaag
     6841 gcaaagacga atggcaggca gttgcgccga gctggcgttt cgatatggag attgaagaag
     6901 atctggtcga agaagtcgcg cgtgtttacg gctacaacaa catcccggat gagccggtac
     6961 aggcaagcct gattatgggg actcaccgtg aagctgacct gtcgctcaag cgcgtgaaaa
     7021 cgctgctcaa cgacaaaggc tatcaggaag tgatcaccta cagcttcgtt gatccgaaag
     7081 tgcagcagat gatccatcca ggcgttgaag ccttactgct ggcaagcccg atctctgttg
     7141 aaatgtcagc aatgcgtctt tctctgtgga ccggcctgct ggcaaccgtg gtgtacaacc
     7201 agcaccgtca gcagaaccgt gtgcgcattt tcgaaagcgg tctgcgtttc gtaccagata
     7261 ctcaggcacc gttgggcatt cgtcaggatc tgatgttagc cggtgtgatt tgcggtaacc
     7321 gttacgaaga gcactggaac ctggcaaaag agaccgttga tttctatgat ttgaaaggcg
     7381 accttgaatc cgttctcgac ctgaccggta aactgaatga ggttgagttc cgtgcagaag
     7441 cgaatccggc actgcatccg gggcaatccg cagcgattta tctgaaaggt gaacgtattg
     7501 gttttgttgg agttgttcat cctgaactgg aacgtaaact ggatcttaac ggtcgcactc
     7561 tggtgttcga actggagtgg aacaagctcg cagaccgcgt ggtgcctcag gcgcgcgaga
     7621 tttctcgctt cccggcgaac cgtcgtgaca tcgcggtggt ggtcgcagaa aacgttcccg
     7681 cagcggatat tttatccgaa tgtaagaaag ttggcgtaaa tcaggtagtt ggcgtaaact
     7741 tatttgacgt gtaccgcggt aagggtgttg cggaggggta taagagcctc gccataagcc
     7801 tgatcctgca agataccagc cgtacactcg aagaagagga gattgccgct accgtcgcca
     7861 aatgtgtaga ggcattaaaa gagcgattcc aggcatcatt gagggattga acctatggcg
     7921 cttacaaaag ctgaaatgtc agaatatctg tttgataagc ttgggcttag caagcgggat
     7981 gccaaagaac tggttgaact gtttttcgaa gagatccgtc gcgctctgga aaacggcgaa
     8041 caggtgaaac tctctggttt tggtaacttc gatctgcgtg ataagaatca acgcccggga
     8101 cgtaacccga aaacgggcga ggatattccc attacagcac ggcgcgtggt gaccttcaga
     8161 cccgggcaga agttaaaaag ccgggtcgaa aacgcttcgc ccaaagacga gtaatctgat
     8221 ctaactaaaa aggccgctat gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa
     8281 atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc gccaacaaca
     8341 gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg cgcttctctt
     8401 aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc ctcgtggcga
     8461 actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg ttggtgcggc
     8521 gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg cagaacctgg
     8581 actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca
     8641 agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac
     8701 tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat tgctggctgg
     8761 cgttgcatta gggattatct gcagcgcact aatgacgtgg gctatctact tttccacctc
     8821 tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg tagactggcg
     8881 gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt gtcagtccag
     8941 gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt tacccatgtg
     9001 gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca gtgtggcgct
     9061 ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt tgtgtggttt
     9121 aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg cattgctgct
     9181 ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg gcgtggtcac
     9241 cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac gttagccgca
     9301 aaaagacggt ctatgattaa aagctaagat ttttactgac cacacctagg agaaacgatg
     9361 caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac cacgctggag
     9421 aagtacgccg gtaatgtgct gttaattgtc aatgtcgcct caaagtgtgg cttaacgccg
     9481 caatatgagc agttggagaa tattcaaaaa gcctgggccg atcgaggttt tgtggtgctg
     9541 ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga gattaaaact
     9601 tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga agttaatggc
     9661 gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc agtcgcgccg
     9721 gaagagagcg gattctatgc ccgtatggtc agcaaaggcc gtgcaccgct gtacccggat
     9781 gatattttat ggaattttga aaaattcctg gttggcaggg acggaaaagt catccagcgt
     9841 ttttccccgg atatgacgcc ggaagatccc attgtgatgg aaagcattaa actggcgttg
     9901 gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc cgcctggggc
     9961 cgctttcagg cgaggttcgg gctggggaaa tcctgcactt ggtggggccg aatggcgcgg
    10021 gtaagagtac cttactggcg caaatggccg gaatgaccag cggtaaggga agcattcagt
    10081 tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat cgcgcctatc
    10141 tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactacctg acactgcatc
    10201 agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg gctcttgatg
    10261 acaaactcgg tcgtagcacc aatcaacttt ccggcggtga atggcaacgc gtacgtcttg
    10321 ctgcggtggt gttgcaaatc acaccacaag ccaatcccgc aggccaattg ctgcttcttg
    10381 atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa attctgagcg
    10441 cgctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac cacacgttgc
    10501 gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt ggacgcagag
    10561 aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt cgccgtctgg
    10621 atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat cccgaaaatc
    10681 gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt acggaatgcg
    10741 tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc ataaagcacc
    10801 gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga acgaccagct
    10861 acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg atcgtttcga
    10921 tttgcatctg ccccgtgaaa ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga
    10981 cgagttgctg cctggtgacc tggtcttttt caaaacgggt tccggacaaa atggtttgca
    11041 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    11101 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    11161 gtatgtcgaa aaattgacgg cgatgataca tagccgtcaa tttactcctg aatacagtag
    11221 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    11281 aaaggccccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    11341 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    11401 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    11461 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    11521 tggcacggtt cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    11581 gcaattattt tcagagcagt tggaattact aaaatcatgc caacactttt ttattcaaca
    11641 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagacaggga
    11701 taattacgca ggcgaactat taaaatatcc gtttattgag ctgttgatta atgagaatta
    11761 cccgcatctc aatgaaggaa aagataatcg cgatcttttg tccttatcgc aaatgtatcc
    11821 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    11881 tttcacccgc gtaatgctgg agaaaggttt tattcagcaa caaatcacgc atcgctcctt
    11941 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    12001 ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt tccatgctct
    12061 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    12121 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacagatagg
    12181 atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc
    12241 actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa
    12301 cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac
    12361 cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt tcgtcgtctg
    12421 ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc ttgctgatgg
    12481 cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga
    12541 tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta
    12601 tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg
    12661 ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg
    12721 tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt
    12781 tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc gtctctggtt
    12841 taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc
    12901 acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc
    12961 gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg
    13021 gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca
    13081 gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca
    13141 ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca tgacggagca
    13201 aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga
    13261 ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct
    13321 acgtgaggag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg
    13381 tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc
    13441 agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga
    13501 tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga
    13561 tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta
    13621 aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc
    13681 cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg gtaattcgcc
    13741 ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc
    13801 tatccgtata acgcataaaa attaataata attgatataa caatgatttc tattatcaat
    13861 attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg
    13921 cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa aggcgagttt
    13981 ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta
    14041 agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat
    14101 aattccagca atctccgtag agccattgcg gatttgctga caaatatcct cacaaacttc
    14161 taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca ccagatgttc
    14221 aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt
    14281 cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat
    14341 ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat
    14401 agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta ccggacagga
    14461 gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc caatcgcgcc
    14521 ccaactgatt aaatcagcaa taaactgacc ggttaccata tcgaggaact cagtcgcggt
    14581 agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt
    14641 tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc cgacaacagt
    14701 tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg
    14761 cagcgactgc agacgggtgg cgtactctat tgcagcggtg agatcgtgga tcgagcaggg
    14821 gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa ttctgcggcg
    14881 ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc
    14941 gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag
    15001 tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca tcacgataaa
    15061 ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat
    15121 aacaaaaaaa caaaccaatg cactagttct ctagtacatt cggcgactaa ggcccatgat
    15181 atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat
    15241 ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga
    15301 ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat
    15361 agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc
    15421 catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata
    15481 cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc
    15541 cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg
    15601 cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt
    15661 tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca
    15721 aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac
    15781 cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc
    15841 acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga
    15901 cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac
    15961 gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata attctatgaa
    16021 agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata aaatgaacct
    16081 tcattttatt ttttataaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    16141 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    16201 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    16261 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    16321 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    16381 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt ccggaatggg
    16441 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    16501 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    16561 tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    16621 tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg acgaaaacgc
    16681 ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt cctttgccgg
    16741 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    16801 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    16861 tcaccgtggc gtggcgctct ccgctggtgt tcagcggatg gtgcgctccg acctcgcatc
    16921 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    16981 ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg atgagtttta
    17041 cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca ccatggggtc
    17101 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    17161 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    17221 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata ttgagtgggc
    17281 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    17341 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    17401 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    17461 aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc cggactggga
    17521 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    17581 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag atgcaacaga
    17641 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    17701 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    17761 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    17821 gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    17881 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    17941 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    18001 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    18061 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    18121 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    18181 cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga ccaacgttga
    18241 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    18301 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    18361 gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct caattggctc
    18421 aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg tgtctgaatt
    18481 gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc gtgccgcgaa
    18541 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagactttgc
    18601 cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca ccgtggtgca
    18661 aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg cgttcagtcg
    18721 ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga catcctgcgt
    18781 taatcgccgt ataatatctt ttcttaacaa aaacttttgt attttatctg aggcagtgcg
    18841 cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga cccgtttacg
    18901 gctaaaaaaa gccgccatct cttccagcga taatgaatga tgcggcgctt tcagcacgac
    18961 ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac aggcatcgtg
    19021 aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat tttcgccgcc
    19081 gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct catccatacg
    19141 gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg ttaattcagg
    19201 ttcatcaaaa taccccataa acacattggg gccacgcgat gcttcttcac cttcgcaacc
    19261 tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg cggcagcgta
    19321 accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg catgcggcga
    19381 actttctgtg gaaccataga cacttaataa tttaatgctg cgctgctggc attcgcgcgc
    19441 cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg aaaggtctgc
    19501 gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc ctagcataca
    19561 ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa tatctaacaa
    19621 cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc ccgttgcgtg
    19681 accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc gcgcgcaata
    19741 agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg gcagaccctc
    19801 ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta tcgccgtggt
    19861 cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg cgggagccag
    19921 tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg gcaggattaa
    19981 atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg cctgacactt
    20041 attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca cggaaaccgc
    20101 accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag gcagttgaaa
    20161 tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca gacagctcgc
    20221 ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca cggcaatttt
    20281 gtctggcatc gcacgagcgg tctgctgcca gtaatcggcc agtgaagcat cgccccatag
    20341 cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca ctttcatttt
    20401 ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca tgattagccg
    20461 tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag aatagtgtta
    20521 ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa atgaatgttt
    20581 cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt acggaattca
    20641 ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac atggaattta
    20701 ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc gcggttgctc
    20761 ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt cccatgatca
    20821 ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc atgatgtcgg
    20881 ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc gggatcttgc
    20941 gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg tattgcgcca
    21001 gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg gcggcagcct
    21061 gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc aggcagaagc
    21121 ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc attgccagac
    21181 cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta atcagcgggc
    21241 gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt tgcgcatggg
    21301 cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg gtatagagaa
    21361 atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg gcaccttcct
    21421 cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata acctctttaa
    21481 caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag cggccaagca
    21541 tcgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca tccccagcct
    21601 gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg cctgggataa
    21661 actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga ttacgcaata
    21721 cggtataaga tgcgtgttga ggaacatctg gttgctcgcg gtgaaaatcg agggtaacgg
    21781 cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct accggcgctg
    21841 atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc ccgccggtca
    21901 tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc ccggctcgtg
    21961 ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg gcatcaaatt
    22021 tgtcatccga catgcgttat gctcccttcc tgctgcgttt acagcgggag atgtggttga
    22081 aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta gatatcgccc
    22141 accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt gatggcgaca
    22201 atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat ccccagcgtc
    22261 aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc acgctccatc
    22321 cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag caccgccgcc
    22381 agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac gccaaccaca
    22441 cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc gcgacagaga
    22501 atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg agaggtatca
    22561 ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg gctgttgatc
    22621 ttttcctaag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat gtgaccatcg
    22681 acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac acttaaccgt
    22741 gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg gtgtttatct
    22801 ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc gtcgttttgc
    22861 tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg gtcggtattt
    22921 tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag ttcagcataa
    22981 cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt ttcttctccg
    23041 taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg cctccggcga
    23101 atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa tgccgaccag
    23161 ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact gatttaccgg
    23221 ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt taatatcgga
    23281 ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat cttcaagcgt
    23341 gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat taatcaccgg
    23401 aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt cgccggaacc
    23461 ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca gcgcttttgc
    23521 ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca ggctgtgcgg
    23581 cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc cgccaacggt
    23641 cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa tggcattgag
    23701 atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt ctggagtgac
    23761 aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca tgatttctcc
    23821 tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa aatagtgtgc
    23881 gatagcggtg cgggggggtc gacggagcaa gctgttttcc ttgccaacaa tcttgcaaaa
    23941 aaaatgcgct tgctaaaaca aaagtgcaat ttgcggcaat atagttattc catgaccgta
    24001 caatcttgca ttcagtgtaa ataatcttgt ttagctctcg ctaaacgcgg ttgtataacc
    24061 gcaatacacg ccgtatttta tccggcatat attgtttttg cttgctcatg gaatagggag
    24121 gttgtcatgt atcaacgctg ttttgataat gccagtgaaa cgctgtttgt cgccggtaaa
    24181 acgccacggc tttcacgttt tgcatttagc gatgatccaa aatgggagtc tggacatcac
    24241 gttcatgaca atgaaaccga gctgatttac gtcaagaaag gggttgcaag gtttaccatc
    24301 gattcttcgt tatatgtcgc acatgcggat gacattgtgg tgatagaacg cggcaggctg
    24361 catgcggtgg cctctgacgt taacgatccg gcaacgacgt gtacctgtgc gctgtacggc
    24421 tttcagtttc agggggctga ggaaaatcag ttactgcaac cgcattcttg tccggtaatt
    24481 gccgcggggc agggaaaaga agtcataaaa accttattta atgagctaag tgtgattttg
    24541 ccgcaaagta aaaatagcca aacatcttcg ttatgggatg catttgccta tacattagca
//
LOCUS       DADLCQ010000054        24514 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000054 DADLCQ010000000
VERSION     DADLCQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.054"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(2..94)
                     /locus_tag="JQG95_002053"
     tRNA            complement(2..94)
                     /locus_tag="JQG95_002053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JQG95_002054"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JQG95_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY5888604.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JQG95_002055"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JQG95_002055"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY5888605.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JQG95_002056"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JQG95_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY5888606.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JQG95_002057"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JQG95_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY5888607.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JQG95_002058"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JQG95_002058"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY5888608.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JQG95_002059"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JQG95_002059"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY5888609.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="JQG95_002060"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="JQG95_002060"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JQG95_002061"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JQG95_002061"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY5888610.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSSFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="JQG95_002062"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="JQG95_002062"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY5888611.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="JQG95_002063"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="JQG95_002063"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY5888612.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="JQG95_002064"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="JQG95_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="JQG95_002065"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="JQG95_002065"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY5888613.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="JQG95_002066"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="JQG95_002066"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY5888614.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JQG95_002067"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JQG95_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY5888615.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVL
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="JQG95_002068"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="JQG95_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY5888616.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="JQG95_002069"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="JQG95_002069"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY5888617.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JQG95_002070"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JQG95_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY5888618.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JQG95_002071"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JQG95_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY5888619.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20889)
                     /locus_tag="JQG95_002072"
     CDS             complement(19876..20889)
                     /locus_tag="JQG95_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5888620.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            complement(20900..21019)
                     /locus_tag="JQG95_002073"
     CDS             complement(20900..21019)
                     /locus_tag="JQG95_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAY5888621.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /locus_tag="JQG95_002074"
     CDS             complement(21029..22738)
                     /locus_tag="JQG95_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY5888622.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="JQG95_002075"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="JQG95_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY5888623.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24514)
                     /locus_tag="JQG95_002076"
     CDS             complement(23682..>24514)
                     /locus_tag="JQG95_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888624.1"
                     /translation="SLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGL
                     MAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKV
                     YGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLE
                     PANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAH
                     GFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA
                     "
BASE COUNT         5780 a         6898 c         6282 g         5554 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctagcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccagttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtacattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgagcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactgg catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat cccgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 cggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctat
//
LOCUS       DADLCQ010000055        24319 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000055 DADLCQ010000000
VERSION     DADLCQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.055"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(18..93)
                     /locus_tag="JQG95_002077"
     tRNA            complement(18..93)
                     /locus_tag="JQG95_002077"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JQG95_002078"
     tRNA            complement(152..228)
                     /locus_tag="JQG95_002078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JQG95_002079"
     CDS             complement(331..1716)
                     /locus_tag="JQG95_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5888625.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQG95_002080"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JQG95_002080"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY5888626.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQG95_002081"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JQG95_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY5888627.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQG95_002082"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JQG95_002082"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY5888628.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQG95_002083"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JQG95_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY5888629.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /locus_tag="JQG95_002084"
     CDS             complement(6327..7457)
                     /locus_tag="JQG95_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY5888630.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JQG95_002085"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JQG95_002085"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY5888631.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQG95_002086"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JQG95_002086"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY5888632.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQG95_002087"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JQG95_002087"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY5888633.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQG95_002088"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JQG95_002088"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY5888634.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQG95_002089"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JQG95_002089"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY5888635.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQG95_002090"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JQG95_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY5888636.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQG95_002091"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JQG95_002091"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY5888637.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQG95_002092"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JQG95_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY5888638.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQG95_002093"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JQG95_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY5888639.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQG95_002094"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JQG95_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY5888640.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JQG95_002095"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JQG95_002095"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY5888641.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JQG95_002096"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JQG95_002096"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY5888642.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQG95_002097"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JQG95_002097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY5888643.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="JQG95_002098"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="JQG95_002098"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY5888644.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JQG95_002099"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JQG95_002099"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY5888645.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..>24319
                     /locus_tag="JQG95_002100"
     CDS             23963..>24319
                     /locus_tag="JQG95_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5888646.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPS"
BASE COUNT         6036 a         6723 c         6034 g         5526 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccggtactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 atattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg tacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtggaatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcg
//
LOCUS       DADLCQ010000056        23876 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000056 DADLCQ010000000
VERSION     DADLCQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.056"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(2..298)
                     /locus_tag="JQG95_002101"
     CDS             complement(2..298)
                     /locus_tag="JQG95_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY5888647.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="JQG95_002102"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="JQG95_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY5888648.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JQG95_002103"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="JQG95_002103"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY5888649.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="JQG95_002104"
     CDS             complement(1784..2323)
                     /locus_tag="JQG95_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY5888650.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="JQG95_002105"
     CDS             complement(2353..3096)
                     /locus_tag="JQG95_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY5888651.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..3213)
                     /locus_tag="JQG95_002106"
                     /pseudo
     CDS             complement(3124..3213)
                     /locus_tag="JQG95_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3453..4091
                     /gene="ompW"
                     /locus_tag="JQG95_002107"
     CDS             3453..4091
                     /gene="ompW"
                     /locus_tag="JQG95_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY5888652.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4151..4657)
                     /locus_tag="JQG95_002108"
     CDS             complement(4151..4657)
                     /locus_tag="JQG95_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY5888653.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4703..5203)
                     /locus_tag="JQG95_002109"
     CDS             complement(4703..5203)
                     /locus_tag="JQG95_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY5888654.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5289..5468)
                     /locus_tag="JQG95_002110"
     CDS             complement(5289..5468)
                     /locus_tag="JQG95_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY5888655.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="JQG95_002111"
     CDS             complement(5849..6655)
                     /gene="trpA"
                     /locus_tag="JQG95_002111"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY5888656.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="JQG95_002112"
     CDS             complement(6655..7848)
                     /gene="trpB"
                     /locus_tag="JQG95_002112"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY5888657.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7860..9218)
                     /gene="trpCF"
                     /locus_tag="JQG95_002113"
     CDS             complement(7860..9218)
                     /gene="trpCF"
                     /locus_tag="JQG95_002113"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY5888658.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="JQG95_002114"
     CDS             complement(9222..10817)
                     /gene="trpD"
                     /locus_tag="JQG95_002114"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY5888659.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="JQG95_002115"
     CDS             complement(10817..12379)
                     /gene="trpE"
                     /locus_tag="JQG95_002115"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY5888660.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="JQG95_002116"
     CDS             complement(12471..12515)
                     /gene="trpL"
                     /locus_tag="JQG95_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY5888661.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12653..13534
                     /gene="yciV"
                     /locus_tag="JQG95_002117"
     CDS             12653..13534
                     /gene="yciV"
                     /locus_tag="JQG95_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY5888662.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13531..14151
                     /locus_tag="JQG95_002118"
     CDS             13531..14151
                     /locus_tag="JQG95_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY5888663.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14252..15124
                     /gene="rluB"
                     /locus_tag="JQG95_002119"
     CDS             14252..15124
                     /gene="rluB"
                     /locus_tag="JQG95_002119"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY5888664.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="JQG95_002120"
     CDS             complement(15164..15754)
                     /gene="cobO"
                     /locus_tag="JQG95_002120"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY5888665.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15751..16509)
                     /locus_tag="JQG95_002121"
     CDS             complement(15751..16509)
                     /locus_tag="JQG95_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY5888666.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16729..17778
                     /gene="sohB"
                     /locus_tag="JQG95_002122"
     CDS             16729..17778
                     /gene="sohB"
                     /locus_tag="JQG95_002122"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY5888667.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17814..18065)
                     /locus_tag="JQG95_002123"
     CDS             complement(17814..18065)
                     /locus_tag="JQG95_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY5888668.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18445..21042
                     /gene="topA"
                     /locus_tag="JQG95_002124"
     CDS             18445..21042
                     /gene="topA"
                     /locus_tag="JQG95_002124"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY5888669.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILCFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21252..22226
                     /gene="cysB"
                     /locus_tag="JQG95_002125"
     CDS             21252..22226
                     /gene="cysB"
                     /locus_tag="JQG95_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY5888670.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22521..22685
                     /locus_tag="JQG95_002126"
     CDS             22521..22685
                     /locus_tag="JQG95_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY5888671.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22688..22855
                     /locus_tag="JQG95_002127"
     CDS             22688..22855
                     /locus_tag="JQG95_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888672.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22969..23064
                     /locus_tag="JQG95_002128"
     CDS             22969..23064
                     /locus_tag="JQG95_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888673.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23228..>23876
                     /locus_tag="JQG95_002129"
     CDS             23228..>23876
                     /locus_tag="JQG95_002129"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAY5888674.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTT"
BASE COUNT         6015 a         5888 c         6088 g         5885 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccga agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccatac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgtc accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaacc
     3241 gcggcaagtg gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa
     3301 tcacattaac caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa
     3361 ttatcatatg taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt
     3421 cacgttttta taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg
     3481 cagtaacaac tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc
     3541 gtgcaggttc tgcaaccgta cgtccaacag aaggtgctgg tggtacgtta ggaagtctgg
     3601 gtggattcag cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg
     3661 acaacattgg tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg
     3721 cgaccggcga tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt
     3781 ttggtgatgc cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct
     3841 tctttgataa tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga
     3901 aagattcctg gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt
     3961 tggttaacat gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg
     4021 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
     4081 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
     4141 ataaagggta ttatttcttc gcttctacgc catcagtttc agaacgaatt aaaaatttct
     4201 cagttgtttg cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
     4261 tcgcttcgat gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg
     4321 aggtgtaaca ggtgatttca aattgctcga agacatatcc gccaatagag cctttaacta
     4381 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
     4441 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
     4501 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
     4561 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
     4621 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
     4681 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
     4741 gggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
     4801 gcttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
     4861 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     4921 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     4981 atttcatgcg cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa
     5041 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     5101 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     5161 aaagcagttg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     5221 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     5281 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     5341 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     5401 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaact accacgatgt
     5461 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     5521 gacactcatt aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata
     5581 gagggaaaat atattttgag gaacattctg gatatattaa caattacctg aggaatatgt
     5641 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     5701 tattactgtt gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt
     5761 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     5821 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     5881 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     5941 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     6001 gatccggggc ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct
     6061 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc
     6121 gtgacagcaa atatgtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     6181 cggcatttgg cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga
     6241 agggcgcgga ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct
     6301 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     6361 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     6421 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg
     6481 ccagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     6541 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     6601 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     6661 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     6721 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcagcgcatg
     6781 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     6841 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     6901 aagatacgcg tgttgtggac cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     6961 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aataaatgcc
     7021 cacgcgacca tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccaccagg
     7081 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     7141 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     7201 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacggacaa tggtcggata
     7261 aggatgcggg ccagccgcgg tgcccagcat atagtgcgcg gtttcataac tgccggacca
     7321 gtcgcgtagc gcctcattac aggcatcttt cagcgtggcg gaaccgctat gtaccgggat
     7381 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt
     7441 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     7501 gccatgctga ccggccccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     7561 caacgcctgc cccagcacct ggttggtttt atgtgcgccg ccgtgcagca aatcttcacg
     7621 cttgagatac agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagtgcggt
     7681 tggacgcccg gcatagtttt tcagcaggtc gttgaactgg gcttgaaatt caggatcttt
     7741 ttgcgcgctg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     7801 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     7861 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt
     7921 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     7981 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     8041 agtgaccagt caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     8101 acgtgctgaa actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     8161 acatgtgctg gcagagcttc acgcagcgta tcgatataca gctgatcttc attaccatgc
     8221 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     8281 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     8341 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     8401 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     8461 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     8521 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     8581 ctgattaccg ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     8641 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc taatgcaatg
     8701 gcgcgctcca gttcctcttc attactgact tcggtcagca cccccatctc cagactgtga
     8761 gcgacggctg caagctggcg atattggtcg tcatccagta ctgaaagcat taataagcag
     8821 gcatcggcct ggtaatagcg cgccagatag atctggtaag ggtcgataat gaagtcttta
     8881 cataaaatcg gctgcggggc gatttggctg acgatgagga ggaaatcaaa gctcccctga
     8941 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     9001 atgcgtgccg gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttacac
     9061 tccagaataa acgccgtgcg tgcaccctgt agcgcatcat aaaaatgtcg tgtgctcggc
     9121 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     9181 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagc
     9241 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     9301 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     9361 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     9421 tcaggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     9481 gtcaattgat agctcttaat tttgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     9541 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     9601 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     9661 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     9721 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat atttcggcgc aaagaggaaa
     9781 catacgccta actcgtccag cgcctggcgc gatttattgg cgttcatatc aagattaata
     9841 ccgaatgccg ccagcagatc cgacgagccg gatttactgg agacgctgcg gttaccgtgt
     9901 ttcgccactt tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata
     9961 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
    10021 aacggcgctg cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca
    10081 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
    10141 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
    10201 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
    10261 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
    10321 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
    10381 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
    10441 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
    10501 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
    10561 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
    10621 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
    10681 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta
    10741 tgaccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
    10801 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
    10861 gtacagcgcg ggctttgtta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga
    10921 ctacaccagc acccgcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
    10981 caatgcaggt gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc
    11041 cgcggcgacg accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccgc
    11101 ttaacgtccc catattcata caggcgcgat aagcgtgcag ggcgtcaaga tcgtgacgca
    11161 gttcgccgac tacgcgagag acgaggtgca tcacatagga ataacggtca actttggtga
    11221 gatcggcgat gtagcggctg ccaggggtgc aaatgcgtgc cagatcatta cgggcgagat
    11281 caaccagcat cagatgttca gacagctctt tatggtcagt acgcatttcc agttcaatac
    11341 ggctgtcgag atctctgtcc agtgaaccat cggcgcgacg accgcgtggg cgtgttccgg
    11401 caatcgggta gatctcaatc tggcggctgg tggcatcata cttgagcgag ctttccggcg
    11461 acgcgccaaa tagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
    11521 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag
    11581 atggcaccac ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc
    11641 caccgaactc ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccactg
    11701 gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac
    11761 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
    11821 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
    11881 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
    11941 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga
    12001 aagcgtcaaa aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag
    12061 gggggaagcg cagcacacgg cagtttggtg attgttcact ttccacaccc gcaggcaggg
    12121 cgttatccag tagtgccagc agggcttcgc cattgccgga aagtgcctga attgtgacgg
    12181 tgtcacctaa tgctgtaatg cgcagcgcac tgtctatcag cagcaggctt tttaaatcat
    12241 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgattc ccacacaact
    12301 ggtgaaaaag cgcagtcggg ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
    12361 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
    12421 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
    12481 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
    12541 tgcgcactag ttaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
    12601 agacccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
    12661 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
    12721 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
    12781 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
    12841 ggcgctgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
    12901 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
    12961 gacagaacgg cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat
    13021 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
    13081 ttttgcgcgt ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa
    13141 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
    13201 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
    13261 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc
    13321 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc
    13381 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
    13441 cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga
    13501 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
    13561 aacccgcagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
    13621 gtctatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgccatg
    13681 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
    13741 gatctttctg aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa
    13801 aacaacacgc cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt
    13861 ctgttgcagg agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    13921 gcgttgcttg aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    13981 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    14041 attattcacg gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat
    14101 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    14161 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    14221 gcctgtgaag gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    14281 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    14341 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    14401 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    14461 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    14521 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    14581 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    14641 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    14701 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    14761 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    14821 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    14881 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    14941 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac
    15001 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg
    15061 tgcggtgaaa cgtcacagtc aggtgagcgg cagtcgtcgt tctggcgggc gtaataacgg
    15121 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta
    15181 tttgcgcctt aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat
    15241 ccgccagttc aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat
    15301 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    15361 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    15421 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    15481 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    15541 cattaggcca ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg
    15601 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    15661 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    15721 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    15781 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    15841 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    15901 tgcgcgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg
    15961 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    16021 accccagttg gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    16081 gtccgatttg agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt
    16141 gacctgcatg acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc
    16201 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg
    16261 tgccagttgt tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    16321 ctgacgccca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    16381 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat
    16441 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    16501 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    16561 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    16621 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    16681 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    16741 tctgaatatg gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt
    16801 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    16861 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    16921 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    16981 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    17041 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    17101 acggcggtac tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct
    17161 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    17221 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    17281 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    17341 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    17401 accgccgggc agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg
    17461 gagaaattcc gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt
    17521 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    17581 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    17641 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    17701 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    17761 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    17821 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    17881 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    17941 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    18001 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    18061 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    18121 tataccacgc tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa
    18181 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    18241 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    18301 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagagcg
    18361 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    18421 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    18481 aaagccaaaa cgatcaacaa gtatctgggt tgtgactacg tggtgaaatc cagcgtcggt
    18541 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    18601 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    18661 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    18721 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    18781 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg
    18841 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac
    18901 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    18961 cgcgtggtgg ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct
    19021 gccggtcgtg tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa
    19081 gcgttcgtac cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa
    19141 gcgttggcgt tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa
    19201 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    19261 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    19321 gccagcaccc gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    19381 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    19441 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    19501 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    19561 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    19621 ttaatctggc gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg
    19681 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gtgctttgat
    19741 ggctggacaa aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca
    19801 gtcgataaag gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag
    19861 ccgccagccc gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt
    19921 cgtccgtcta cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta
    19981 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    20041 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    20101 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    20161 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    20221 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagt
    20281 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    20341 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    20401 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    20461 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    20521 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    20581 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    20641 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa
    20701 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    20761 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    20821 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    20881 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    20941 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    21001 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    21061 ttttataaac aaagggtcgc gaaagcggcc cttttttatt gcatattatt ttttcttcac
    21121 acctatacac taaggctata aatgatatag tggttatagt tagcaccttt tttattatta
    21181 aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg
    21241 gatggtttaa catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc
    21301 tgaatgtctc atcaacagcg gaaggacttt acacatcaca acccgggatc agtaaacaag
    21361 tcagaatgct ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga
    21421 cgcaggtaac gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag
    21481 tcgatgccat aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata
    21541 tcgccaccac gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg
    21601 agcgttatcc ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg
    21661 ccgtctctaa aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag
    21721 atttagtgat gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc
    21781 cgctggcagg caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat
    21841 ataccttcgg ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa
    21901 cgccgcgtat cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag
    21961 ggctgggggt aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg
    22021 tgcgcgttga tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta
    22081 gtactttttt gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc
    22141 gtgatgtcgt tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta
    22201 aagatataaa actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc
    22261 gaaggtgccg gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta
    22321 tccctcatat ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa
    22381 ttccagtttt caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc
    22441 gtaaattcgc tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat
    22501 tacaccaatc attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc
    22561 cttctggaaa tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg
    22621 tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg
    22681 gataactatg gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa
    22741 tgaaccagaa acgaaacggg aatgttccgt cgttattcca gacgactggc aactaacatc
    22801 gcagcagcaa gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc
    22861 atcattctta ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac
    22921 agcttgagtt atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca
    22981 aatatgaaat attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa
    23041 ctcctctgga tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt
    23101 tgccacaagg tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt
    23161 tgtgttatct ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg
    23221 aggagctatg tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa
    23281 aacttaccac tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct
    23341 acccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt
    23401 taccgaagag gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga
    23461 aattgcctac cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt
    23521 tgatctggcg gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa
    23581 cccgctctca ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga
    23641 tgatgaggca tttgaagaaa acgtacgcct ggaaatggag cgcaaccacg aacgttatgt
    23701 gttcctgaaa tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg
    23761 catttgccat caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga
    23821 cggtgaatgg attgcttatc cggatacact cgttggtact gactcgcaca ccacta
//
LOCUS       DADLCQ010000057        22771 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000057 DADLCQ010000000
VERSION     DADLCQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.057"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(22..594)
                     /locus_tag="JQG95_002130"
     CDS             complement(22..594)
                     /locus_tag="JQG95_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888675.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JQG95_002131"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JQG95_002131"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY5888676.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JQG95_002132"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JQG95_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY5888677.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JQG95_002133"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JQG95_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY5888678.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JQG95_002134"
     CDS             complement(3556..5337)
                     /locus_tag="JQG95_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY5888679.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JQG95_002135"
     CDS             complement(5330..7102)
                     /locus_tag="JQG95_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY5888680.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7677)
                     /locus_tag="JQG95_002136"
     CDS             complement(7132..7677)
                     /locus_tag="JQG95_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY5888681.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGVWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JQG95_002137"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JQG95_002137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY5888682.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRECDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8779..10353
                     /locus_tag="JQG95_002138"
     CDS             8779..10353
                     /locus_tag="JQG95_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY5888683.1"
                     /translation="MLRQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALE
                     TGKQQDVLELAQLAPGELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAE
                     QHLAGQIFSGLTRFDNNTQRPIGDLAHHWETSTDRLRWDFYLRSTLHWHNGDAVKASH
                     LHQRLLMLLQLPALDQLFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQ
                     FPLIGTGPFRLTQFTAELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPV
                     QITIGKPEELQRVSQVSSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEV
                     GENLITASHALLLGWTIPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGC
                     ELTIIFHNAKNWDDTTLLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYA
                     HLQSTLDAVQVMPDEENRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTP
                     RGWFEFTEAWLPAPSQ"
     gene            10418..11113
                     /gene="queC"
                     /locus_tag="JQG95_002139"
     CDS             10418..11113
                     /gene="queC"
                     /locus_tag="JQG95_002139"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY5888684.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11165..11563)
                     /locus_tag="JQG95_002140"
     CDS             complement(11165..11563)
                     /locus_tag="JQG95_002140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY5888685.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11657..12028)
                     /locus_tag="JQG95_002141"
     CDS             complement(11657..12028)
                     /locus_tag="JQG95_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888686.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="JQG95_002142"
     CDS             complement(12179..14050)
                     /gene="ppiD"
                     /locus_tag="JQG95_002142"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY5888687.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="JQG95_002143"
     CDS             complement(14242..14514)
                     /gene="hupB"
                     /locus_tag="JQG95_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY5888688.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14723..17077)
                     /gene="lon"
                     /locus_tag="JQG95_002144"
     CDS             complement(14723..17077)
                     /gene="lon"
                     /locus_tag="JQG95_002144"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY5888689.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="JQG95_002145"
     CDS             complement(17265..18539)
                     /gene="clpX"
                     /locus_tag="JQG95_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY5888690.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="JQG95_002146"
     CDS             complement(18665..19288)
                     /gene="clpP"
                     /locus_tag="JQG95_002146"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY5888691.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19535..20833)
                     /gene="tig"
                     /locus_tag="JQG95_002147"
     CDS             complement(19535..20833)
                     /gene="tig"
                     /locus_tag="JQG95_002147"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY5888692.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="JQG95_002148"
     CDS             complement(21177..21494)
                     /gene="bolA"
                     /locus_tag="JQG95_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY5888693.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21799..22377
                     /locus_tag="JQG95_002149"
     CDS             21799..22377
                     /locus_tag="JQG95_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5888694.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22421..>22771
                     /gene="ampG"
                     /locus_tag="JQG95_002150"
     CDS             22421..>22771
                     /gene="ampG"
                     /locus_tag="JQG95_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="in Escherichia coli this protein is a permease
                     involved in peptidoglycan recycling; member of major
                     facilitator superfamily; MFS; inner membrane protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY5888695.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVI"
BASE COUNT         5402 a         6327 c         5462 g         5580 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcattcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 taatattgtt ccgggtttat atgcgattgc tcaaccgtct taaccagtat caacgtctgt
     8701 ggcaaccttc agccgcaaac cgtcaccgtc agcgaactgg ccgaacgctg tttttgcagc
     8761 gaacgccatg ttcgtacgct gttgcgtcag gcacaggagg cgggatggct ggagtggcag
     8821 gcgcagtcag gacgcggaaa gcgcggacaa ttacgctttc tggtcacgcc ggaatcgcta
     8881 cgcaatgcga tgatggaaca ggcactggaa accggaaagc agcaagatgt gctggagctg
     8941 gcgcaactgg ccccaggtga gctgcgcact ctgttacagc catttatggg cggacaatgg
     9001 caaaacgata cacccacgtt gcgtattccc tactatcgcc cgctcgaacc gctacaacca
     9061 ggctttttgc ccggccgtgc cgagcagcat ctcgccgggc agatattttc cggcctgacc
     9121 cgcttcgata ataatactca gcgcccgatt ggcgatttag cgcatcactg ggaaacctct
     9181 actgacaggt tacgctggga cttttatctt cgttcaaccc tacactggca taacggcgat
     9241 gcagtaaaag cctcacactt acaccagcga ttattgatgc tgttacaact gccagcactg
     9301 gatcaattat ttattagcgt gaagcgtatt gaagtcaccc atccgcagtg tctgaccttc
     9361 tttttacatc gccccgatta ctggcttgcg caccggctgg cgagctattg cagccatctg
     9421 gcgcatccgc aattcccact gatcggcact ggtccttttc gcttaacaca attcacagcg
     9481 gagctggtgc gcctggagag ccatgattat taccatttgc gtcatccgct gcttaaagcg
     9541 gttgagtact ggataactcc gccgcttttc gaaaaagatt tgggaaccag ttgtcggcat
     9601 cccgtgcaaa tcaccatcgg caaaccggag gagctgcaac gggtcagcca agtcagtagt
     9661 ggcatcagtt taggtttttg ctatttaacg ttgcgcaaaa gtccccgact ctccctctgg
     9721 caggcgcgaa aagtgatctc cattattcat caatccggtt tattacaaac gttagaagtc
     9781 ggagaaaacc tgatcaccgc cagtcatgca ttactgctag gctggactat tccgcactgg
     9841 caagtaccgg atgaagtcaa actaccgaaa accttgacgc tggtttatca cctaccgata
     9901 gaacttcata ccatggcaga acgcctacag gcgacactgg cagcggaagg ctgtgaactc
     9961 acaattattt ttcataacgc aaaaaactgg gacgacacga ccctactggc acacgcagac
    10021 ctcatgatgg gcgacagatt aattggcgaa gcaccggaat atactctgga gcaatggcta
    10081 cgttgcgatc cactatggcc acatgttttc gacgctccag catatgcaca tctgcaatcg
    10141 acgctggacg cggtgcaagt aatgcctgat gaggaaaacc gatttaatgc cctgaaagcg
    10201 gtttttagcc agttaatggc agacgcgacg ctgacgccgc tgttcaacta tcactatcgc
    10261 attagtgccc ctcccggcgt gaacggtgtg cgactgacac cgcgcggctg gtttgaattt
    10321 accgaagcct ggcttcccgc gccgtcgcaa tgaatagctg gtccgggtta atcgcaggcg
    10381 ttaccataac gcttttattg ttcaagcagg attatctatg aaacgtgctg tcgttgtgtt
    10441 cagtggaggc caggattcca ccacctgtct ggtgcaggca ttacaacaat atgatgaagt
    10501 ccattgcgtg acgttcgatt acggtcagcg acatcgcgca gaaatcgacg tggcacgcga
    10561 actggcgctg aaactgggag cacgcgcgca taaggttctt gatgtaacgc tgctcaacga
    10621 gctggcggtc agcagcctga cacgtgacag cattccggtg cctgattatg aacctgaagc
    10681 cgatggcatc ccgaatacgt ttgtcccagg gcgtaatatt ttgttcctga cgctggcggc
    10741 aatatatgcg tatcaggtaa aagcagaagc cgtaattacc ggcgtctgtg aaacggattt
    10801 ctccggctac ccggattgcc gcgatgagtt tgtgaaagca ctaaaccatg ccgtcagttt
    10861 gggcatggcg aaagatattc gttttgaaac gccgctgatg tggattgata aagcggaaac
    10921 ctgggcgctg gcagattatt acggcaaact ggatttagtc cgtaacgaaa cgttgacctg
    10981 ctataacggc attaaaggcg acggttgcgg tcattgtgcg gcatgtaatt tacgcgccaa
    11041 cggtttgaat cattatctgg ccgataaacc gacggtgatg gcagcgatga agcagaaaac
    11101 cgggttgagg taattattcc gggtgtcgcc ggatgcggct tgagcatccg gcaccacaaa
    11161 acgtttactt aaccatctgc tccagctttt cgcgcaattc cccttccaga gctaatgctt
    11221 tctgcgtttt aagatcaata caaacaaacg taataagcgc atccgctacc acctgccctt
    11281 ccggctccag tgtaatgacc tggtttaaga tgccgctttt accgtttaat tgctgcaact
    11341 gactggtaat agttaacagg tcgcttaata ccgctgggcg acgatagtta atattgatat
    11401 tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg ctattttcca
    11461 ccccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg acgtgctggt
    11521 aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa taaccttacg
    11581 ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg caaaaaattg
    11641 ccactatgca aattaattac agggttaata ccgccagatt acgttccacc agcgaattgc
    11701 ccatccccgg cacctgcttt agatcctcca ccgttttaaa cggaccgtac tcttcgcgat
    11761 aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg gctagctctt
    11821 ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct ttcgccggta
    11881 ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc gtcggtttcg
    11941 ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg gacagggtaa
    12001 tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta acagtggaat
    12061 caccttaacg ggagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg aaaaggccgc
    12121 gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgg cttcaggatt
    12181 attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg ttactcatca
    12241 gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc gcttttttct
    12301 gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc acattacctt
    12361 gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc agtgcaaacg
    12421 ccgcctggct aatcgggtca cgaccggaac ggcttaaggt tttcggctcg ccaaatttca
    12481 gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc agcagtttct
    12541 cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc ttaacttgtt
    12601 cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgacg cgcagcacga
    12661 atgcgcggtc gccgtctacg gtgatgatgt cagagttgat gcccgacgcg ccgttttcac
    12721 ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac tcttccggca
    12781 ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcagct tgctctgcac
    12841 cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc agcgcatagt
    12901 acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt acttcgtcta
    12961 acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa ccgaccgaag
    13021 atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc agttcgtcgg
    13081 ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag ataatatcgg
    13141 cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca tcaagtaccg
    13201 ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc tgcggctggg
    13261 tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta accggttgct
    13321 gcatcgttgc ggcaaccagc ttgatgtaac tcacgcggaa ttgttccggc gtcatgaaat
    13381 tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc tgcttcgccg
    13441 ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg accagtgccg
    13501 ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacgccg ttaatcagct
    13561 gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc atccccatct
    13621 ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg aaggctgggg
    13681 tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc tcacgagcgt
    13741 actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc agggttttca
    13801 tatagccttc gttcgctgcc agctcggagt attgatcgcc cagctgttgc tgcatacgat
    13861 tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg tcattcactt
    13921 ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc aatatgaacg
    13981 acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc gtgcgtaagc
    14041 tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac gcaacgtatc
    14101 gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc acaagacaca
    14161 gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg aacttcgtca
    14221 catccccact ggggacaacg cttagtttac cgcgtctttc agtgctttac ctgcacggaa
    14281 gctcggtact ttagcggcag cgatggtgat ctctttaccg gtctgcgggt tgcggccagt
    14341 acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta catcatcccc
    14401 ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc cagccgcagc
    14461 tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat tcactcttct
    14521 cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc gcagcagtta
    14581 tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga tccgctatct
    14641 aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc ctgtttttat
    14701 tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca gacggttcat
    14761 tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg tccagatcgg
    14821 caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga attagcactg
    14881 ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg atcggcagta
    14941 cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc gggttaccgg
    15001 tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca tctttcggcg
    15061 tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca gggttgatcc
    15121 ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac tcctgcatca
    15181 cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat gcggtttcaa
    15241 tggtcagcaa gtcaccgccc acttccgtcc acgccagacc gattacctga ccgacacggt
    15301 tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca tgcaggttat
    15361 cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta accgctttgc
    15421 ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg gtgtagtaac
    15481 gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc agtgcattac
    15541 gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca tcttcggtat
    15601 aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg ttcatggagt
    15661 tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg tagtggtcgc
    15721 tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc ggatcgcctc
    15781 gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcggattt ttcacgccca
    15841 ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta cggcggtgac
    15901 cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat ttacgcccgg
    15961 tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc cctaccaggc
    16021 agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac tcaaggatgc
    16081 gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc gcctgacgca
    16141 ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag tcgatataac
    16201 cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc tgcaactctg
    16261 cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg cgcttcaggg
    16321 cttcgttttc gtccggcacg tcgtccattt caccgagttc tttctgaata gctttcattt
    16381 gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg cggttgcgaa
    16441 tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc agatattcca
    16501 gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt ttcagcggca
    16561 tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc agcgacgtca
    16621 gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg ctgattgcag
    16681 tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga tactccgcct
    16741 tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc tgtaacccct
    16801 cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata gaggccacgg
    16861 tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct ttctgcgcga
    16921 ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata gatttttccc
    16981 gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc agcggcaata
    17041 cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt agtttaattt
    17101 ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat gtacgtcagt
    17161 atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta aaggggagat
    17221 aaaatccccc ctttttggtt aactaattgt atgagaatgg ttaattattc accagatgcc
    17281 tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatc aattaccgac
    17341 tcgtcgataa ccactttttc gacgtcttcc atggacggca gatcgtacat ggtatcgagc
    17401 agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc catcgctttc
    17461 ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc cagattaaac
    17521 agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg aatcagagct
    17581 tcttcgctca gttcattcaa cgttgctaca accggcagac gaccaatgaa ctcagggata
    17641 agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc ttcgcttgct
    17701 ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggagccggt ttctacacgg
    17761 tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag gatcttagag
    17821 gtatcaacct gcaagaattc ctgctgcgga tgtttacgcc caccttgcgg tggaacagca
    17881 gctaccgtac cttcgatcag tttcaacagt gcctgctgta ctccttcacc ggaaacgtct
    17941 cgggtaatgg acgggttgtc tgacttacga gaaatcttgt cgatttcatc gatgtagaca
    18001 atcccgcgct gcgctttctg tacgtcgtaa tcgcactttt gcaacagctt ctgaatgatg
    18061 ttttcaacgt cttcgcccac ataaccggct tcggtcagcg tggttgcgtc ggccatggtg
    18121 aacgggacgt ccagcaggcg cgccagcgtt tcagccagca gcgttttacc ggaaccggtc
    18181 ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt atcgccgttg
    18241 cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc ctgttcctgg
    18301 ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag cgcactgcgt
    18361 tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca taaatcaaca
    18421 cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc atgctggctt
    18481 ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt atctgtcatg
    18541 ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc cattctcagg
    18601 cgcaagccgc ttacagcgtt gtgccgccct agataagtat agcggcacag ttgcgcctct
    18661 ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc gcttcagggg
    18721 cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat tgacccgtat
    18781 gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg gcatgaattt
    18841 cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc acgcgcgaat
    18901 tcggcaggca aaaacgttta ccttttgccc ctgcggtcaa caagaaagcg cccatcgagg
    18961 ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg gtgtcataga
    19021 tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag atatcttttt
    19081 ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc atgtggtctt
    19141 caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag atatcaaaag
    19201 agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata tggggtgcaa
    19261 agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg aaaaaaacct
    19321 gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt atgggtacgg
    19381 catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga aagaggcttt
    19441 tcgcacggct ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc ggtgacgggc
    19501 ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc atcagctcgt
    19561 tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca acagcctgtt
    19621 cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg tagaactcga
    19681 taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct ttcacgcgct
    19741 cttcgtcagc tttcagctcg ttggtgcgga taacttcgcc cagcagcagg ccaacaacta
    19801 cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt
    19861 tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg tcgatcagcg
    19921 cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga gacttaacgc
    19981 ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca gcgcgcagac
    20041 cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc agttccggca
    20101 gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta cctttcaggt
    20161 tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg ccagctttgt
    20221 ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc gccagtacga
    20281 aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg aagtcgatgg
    20341 ttacgcggtc ttctgcttca acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac
    20401 gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa
    20461 cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac tctacagagt
    20521 aagtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg ccagccggat
    20581 tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca cccagaacgt
    20641 cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact ttgcctttgc
    20701 ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta acagcggtct
    20761 cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga gtggtttcaa
    20821 ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca
    20881 agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac attccctgtt
    20941 accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca tcacttttat
    21001 gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg ggcaattttc
    21061 gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt
    21121 tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat gcgtttttac
    21181 gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg caacccttcc
    21241 cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga gagttcctcc
    21301 gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg
    21361 ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg acgatagctt
    21421 tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc ttctatccgc
    21481 tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca tccctttaaa
    21541 tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg tttcgtcctg
    21601 gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag gcctcgcatc
    21661 agcgtttctt ttaccagggg tgattatttc gtcaacatga tgaatttaca tgccttaccc
    21721 acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac acgcttaaga
    21781 gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg
    21841 cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat tacactgcca
    21901 cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga tcagcgtacc
    21961 gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc
    22021 gctctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg
    22081 gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta tgctgacgtg
    22141 tcccagggca acgtgcgcta caacatcgct accaaagcgg atatcgccat catcgctacc
    22201 gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg
    22261 ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac cgataccatc
    22321 gctgatatgt ctcaggacac cagcattcac gaattcatca agcagaacgc gcgttaattt
    22381 ctgccctctg gcccggtgca agccgggcct gtagacgccc atgtccagtc aatatttacg
    22441 tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt ccgggctacc
    22501 gctcgccctg acatccggca ccttacaggc ctggatgacg gtcgaaaata tcgatctcaa
    22561 aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc tctggtcacc
    22621 gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc tgctcgccac
    22681 gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaaccag gcacccaact
    22741 ccgctggatg gcggcgctgg cggtggtgat c
//
LOCUS       DADLCQ010000058        22337 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000058 DADLCQ010000000
VERSION     DADLCQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.058"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            208..747
                     /locus_tag="JQG95_002151"
     CDS             208..747
                     /locus_tag="JQG95_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY5888696.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            1045..1929
                     /locus_tag="JQG95_002152"
     CDS             1045..1929
                     /locus_tag="JQG95_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY5888697.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(2106..2453)
                     /locus_tag="JQG95_002153"
     CDS             complement(2106..2453)
                     /locus_tag="JQG95_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY5888698.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2582..3553)
                     /gene="secF"
                     /locus_tag="JQG95_002154"
     CDS             complement(2582..3553)
                     /gene="secF"
                     /locus_tag="JQG95_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY5888699.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3564..5411)
                     /gene="secD"
                     /locus_tag="JQG95_002155"
     CDS             complement(3564..5411)
                     /gene="secD"
                     /locus_tag="JQG95_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY5888700.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5439..5771)
                     /gene="yajC"
                     /locus_tag="JQG95_002156"
     CDS             complement(5439..5771)
                     /gene="yajC"
                     /locus_tag="JQG95_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY5888701.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5794..6921)
                     /gene="tgt"
                     /locus_tag="JQG95_002157"
     CDS             complement(5794..6921)
                     /gene="tgt"
                     /locus_tag="JQG95_002157"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY5888702.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(6976..8046)
                     /gene="queA"
                     /locus_tag="JQG95_002158"
     CDS             complement(6976..8046)
                     /gene="queA"
                     /locus_tag="JQG95_002158"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY5888703.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            8139..8712
                     /gene="acpH"
                     /locus_tag="JQG95_002159"
                     /pseudo
     CDS             8139..8712
                     /gene="acpH"
                     /locus_tag="JQG95_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(8717..10534)
                     /gene="malZ"
                     /locus_tag="JQG95_002160"
     CDS             complement(8717..10534)
                     /gene="malZ"
                     /locus_tag="JQG95_002160"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY5888704.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(10690..12063)
                     /gene="proY"
                     /locus_tag="JQG95_002161"
     CDS             complement(10690..12063)
                     /gene="proY"
                     /locus_tag="JQG95_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY5888705.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(12139..13458)
                     /gene="brnQ"
                     /locus_tag="JQG95_002162"
     CDS             complement(12139..13458)
                     /gene="brnQ"
                     /locus_tag="JQG95_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY5888706.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(13865..15160)
                     /gene="phoR"
                     /locus_tag="JQG95_002163"
     CDS             complement(13865..15160)
                     /gene="phoR"
                     /locus_tag="JQG95_002163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY5888707.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(15218..15907)
                     /gene="phoB"
                     /locus_tag="JQG95_002164"
                     /pseudo
     CDS             complement(15218..15907)
                     /gene="phoB"
                     /locus_tag="JQG95_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
     gene            16097..17299
                     /gene="sbcD"
                     /locus_tag="JQG95_002165"
     CDS             16097..17299
                     /gene="sbcD"
                     /locus_tag="JQG95_002165"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY5888708.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQMDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     AFGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            17296..20439
                     /gene="sbcC"
                     /locus_tag="JQG95_002166"
     CDS             17296..20439
                     /gene="sbcC"
                     /locus_tag="JQG95_002166"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY5888709.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLIVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            20565..21746
                     /gene="araJ"
                     /locus_tag="JQG95_002167"
     CDS             20565..21746
                     /gene="araJ"
                     /locus_tag="JQG95_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY5888710.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGGAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(21889..>22337)
                     /locus_tag="JQG95_002168"
     CDS             complement(21889..>22337)
                     /locus_tag="JQG95_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY5888711.1"
                     /translation="WGHNPLPWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYR
                     RLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSN
                     VDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAWLWPQE"
BASE COUNT         5649 a         6028 c         5569 g         5091 t
ORIGIN      
        1 cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg
       61 gcgtcctgac caggtggtta acggaaatct attttaccct gaactgacga tacaacaaag
      121 gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc taccattagg
      181 ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact gctcctgctg
      241 ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg aagtgcgaca gatgaaacac
      301 agcgtcagca cgctgaacca ggagatgacg caactcaatc aagaaacagt caaaatcact
      361 cagcaaaaca ggctgaatgc aaaatccagc agtggggttt accttctgcc tggagcgaaa
      421 acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt gaatattacg
      481 cctgatacag atggcaccac ccttacacta cgtattcagg gtgagtcgaa tgatcccttg
      541 cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga caattttcag
      601 gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc ccccagcgat
      661 gttgatattc cgttacaatt aaaggggatt tctgtggatc agttaggctt tgtacgtatc
      721 cacgacattc aacctgttat gcagtaaacg tatttcggga cgattttgtg cgtcccgcaa
      781 catcttcccc gtcattttgt tactctgctt acatcacctg gattgatagt aaaagtttgc
      841 aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga acgcaatcga
      901 ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga tttttataat
      961 aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca ctcccgcaag
     1021 ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg tgcggtactg
     1081 gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca gtatctttcc
     1141 gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt cggaccgcag
     1201 atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg gttcgacttc
     1261 tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa aggtatctgg
     1321 aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga caagctgacc
     1381 aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa ctacatttac
     1441 gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct gggtaccgat
     1501 atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata ccagtggcag
     1561 aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat taaatacttt
     1621 gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac caacttcgac
     1681 tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa gacccgtact
     1741 aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg gcactactct
     1801 gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga actgaacttc
     1861 ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt agtaggttac
     1921 aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt taacaacgtg
     1981 tgctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa gcacgtaaat
     2041 tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc aattttacgt
     2101 tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt gtacttcgct
     2161 tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata ctgatccgct
     2221 tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc ccagttactg
     2281 ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag ttcacgcagg
     2341 ttagaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat ttttaatgct
     2401 ttttcgcgat agccgctttc taaccgtgca tagttttttg ggatgatagc catgaaaagt
     2461 tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt ttcgcaatct
     2521 ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa cgggaacttg
     2581 attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc tgcaacatgt
     2641 gttcgcgctt catacccagt ttcagagcca acgcagatgc tacgtagatg gaagatgcag
     2701 tgccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc ggaccaccga
     2761 agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag gtacggtgca
     2821 gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg atcttgcgga
     2881 agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa ccgataaccg
     2941 acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac aaaataccca
     3001 gcgtaatgat aacgtcgtgc gtcagcgcaa taaccacccc tgccgccagt cgccactcaa
     3061 agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc atcgcaccgg
     3121 tttgcgcaag gtcagccccc acgctcggac cgacgaactc aatacgcttc actgctgcat
     3181 tctgattggt ggattcgtta atcaccttca gaacttggct gcccagcacc tgaccgccgg
     3241 tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca aagttttgca
     3301 gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca atttcagccg
     3361 gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc cagttaaagc
     3421 cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg ccgaaagccc
     3481 agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt tcaacagtat
     3541 attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt gccgccatat
     3601 agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt cgccacaccg
     3661 ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta caggatgatg
     3721 actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc acgataacct
     3781 tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat acgttcgttg
     3841 atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc gatacctggc
     3901 atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat caagttggca
     3961 atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat gaacagaata
     4021 gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg catacccaag
     4081 gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc accggcacgc
     4141 agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat acggaagctg
     4201 ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt caccagaacc
     4261 gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag ggttgccatc
     4321 ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc gctatcgagc
     4381 gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc ggtgatatgg
     4441 tcaccagtca gaattacgcg tttgtacagc acaaccggct gaccttcgcg ggtctgtttc
     4501 acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac attggtgttt
     4561 accagacgga attccagcgt tgccgtcgcg cccagaatct ctttcgcacg cgcagtgtcc
     4621 tgaataccgg gcagttcaac aacgatacgg tcagcaccct gacgctgaac caccggctcc
     4681 gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg caccgcatat
     4741 tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt gctgccctgg
     4801 ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc atcacgagct
     4861 ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt ctttacgaac agtggtatac
     4921 gggatgccct tttcgcgcag gtcactgcgt aggctatcga tattttgttc ctggagtttg
     4981 ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg caggtcaagg
     5041 ccgagcttca tcggctcagc atgaatagct gccagccagc gcggcgttgc cggggcaagg
     5101 ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg agcgcgcaac
     5161 tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc cacagactta
     5221 gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg ctcactggcg
     5281 gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag gttgggaagc
     5341 gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca caaaggataa
     5401 cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt catggtgcct
     5461 ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt gtcattcagc
     5521 gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag gccaccgttc
     5581 gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc tttggtgcgc
     5641 ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac cagcatcaaa
     5701 atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc atcagaaata
     5761 aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag gtggtacttc
     5821 tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct cttcaatagc
     5881 cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg tgttgagtcg
     5941 cgcgcctaat atttcgttgc aacggtcgag atgatgcaag taagcgcgtg aataattgcg
     6001 acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat atttcgcatt
     6061 gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt tgcgggttgg
     6121 cattacgcag tcaaacatat cgataccgcg acgtacgcct tcaaccaggt cttccggttt
     6181 accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcggacaca catgctccag
     6241 aatacggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag cgtagccatc
     6301 aaagccgata tctaccagac ctttaacaga tatatcacgt aaatcttcgt aaatactgcc
     6361 ctgaattata ccaaataacg catttttgtt accaagactg tcaaaacgct cacggctacg
     6421 cttcgcccaa cgcagagaca tctccataga gcgttttgcg taatcccagt cagcaggata
     6481 cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact gaatctccat
     6541 cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga agtgaacgcc
     6601 ctgttcggtg attttacgaa tatcaccaag gctgaagacc tggaagccgc cggagtcggt
     6661 aagaatcggt cctttccact gcataaaatc gtgcagatcg ccgtgcagtt tcatgatttc
     6721 ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg cgccagtggc
     6781 ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag gcataaaaca
     6841 aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc gaccgtcggt
     6901 ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc caacgtttaa
     6961 accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat tgtacgtgat
     7021 aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg ctttataggc
     7081 attcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg tcgactctgg
     7141 caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg gatagataaa
     7201 gatttgggta tcgtcaaaga acggttcgat aagatcgttt tttgccgcct gtgccgcgct
     7261 ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc gcgctttcgc
     7321 cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt gcatgatgtg
     7381 atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt gcaacgtcac
     7441 aaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt caaaatgcag
     7501 accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt gataaagttc
     7561 gcggtcagcg tcttcgtccg gacgatcgat atacggcggc agcggcatat ggccgatgct
     7621 gttgaggata tccagtaccg agcgttcatc attaaattcg acttcaaaca gtgcgccgtg
     7681 gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc agcaacagtt ctgcgccagg
     7741 ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga gcatccgctc
     7801 aaccagcact tcaatcttgc cgccgctggc tttacgccca aacagacgcg ccgggatcac
     7861 gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta aatcggtgaa
     7921 agtaccgtgc gtcagcgcgc ccgtcggccc gtccagcgac agtaaacgac agctactgcg
     7981 ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga aatcggtaac
     8041 gcgcatgaca ctgactcaga ctaaaataag agtcgggtag tctagtgccg gggcgctctc
     8101 cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta gctcacctgc
     8161 atttagccca tctcgcggaa agctcgcttt ctggcaattt actggctgat ttcgtacgcg
     8221 gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg catcgacgta
     8281 ttgactgaca atctgccgga agtccgcgaa gcacaggagt ggtttcgtag tgaaacgcgc
     8341 cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg
     8401 cagctgtcgc cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg
     8461 acgattttgc cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag
     8521 cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca
     8581 agccgccgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat
     8641 gacgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac
     8701 aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa
     8761 tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca ttgagaaacg
     8821 gtgacgcggg tagcaccact tcactggcct cgccacggtt gattgccacc agtacacgtt
     8881 gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc acctgacagc
     8941 cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg tacagcgcga
     9001 ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt
     9061 ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca ggccaggtga
     9121 acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc
     9181 gcgcagtatc gtggctatcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc
     9241 ctgcgcggta attatccatc caggccatac aggtttgggc gtcaatttgc tgcggatcat
     9301 aagagatgtc ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca
     9361 tggcagcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga
     9421 caatatacgc ttccggctgg gtttctttcg ccgcttcggt gatcccggca acgtgctgca
     9481 tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc cgccagccgt
     9541 ccatattcca cggcgctttc agccagtggc ggacaatact gtcttccccg cgataaattt
     9601 cattcaccag actttccgac tgataatcca gcttcgtcaa gctggcatag ccaagccagt
     9661 cgagcgccgt gccatcatca ctaaacgagt accagtcgcg ccagggcgat tcagagttgt
     9721 gacaagctcc ccccgtgcca agattgtgcc tgtcaaacca ggcatgggaa tcgccactgt
     9781 ggttaaacac gccgtccagc accagccgca ttcccagctg ctgcgtattg tgtcgcaaac
     9841 gcagcaacac cccatcaccg ccaaactgcg gatcgacatg gcgataatcc tcggtatcgt
     9901 atttatgtac gctgggagct ttaaacaccg gattgagata cagcgctgtc acgccaagct
     9961 ttttcagata cggcagtttt tcgcttatcc cgtccagatc gccgccatag aacgttgatc
    10021 cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa gatgatctct tgtccggctg
    10081 catgatggta atagacatga tcctgttcag cttcacgagg aagactacgc gcaaaacgat
    10141 cagggaagat ctgataaaaa atctgatccg cagcccattg tgggccgata tcaggtacat
    10201 cgacggcaaa ctgctccagt cgtgccggcg gcattcggct gaagccctgc ggtgtaaacc
    10261 aacgctggcg atcgtgccac agcagtttga aactgtaacg ccgccggggt tgtccgctgg
    10321 agagatcaat cgccgcacgc catgcggtga cgcctggctg cggctgactg cgctgcttat
    10381 gcattggtac tgacatttct tcgttatcgt gttctgtacg cagcataatg cgctgcggtg
    10441 ggtcttcgcc cgtcagccac agtgtaatga gcagttgatc tttgctttgt ttaacaaatg
    10501 ggggcaccgg caggtgccat gcatttaaca tcataattcc cctgtgatga aaatctgatc
    10561 accttgccat agcctaatgc aggatcactc atcatctgaa ggatatttgg gggaggagga
    10621 cgggggcgga taactttcgg aatagccgga tcaggatttc ctcctgaacc ggcttacaga
    10681 acgcaagtat tattggtttt cagccagctg acgatcgtgg cggcgtttaa acatccagcc
    10741 aatcaacagc acaacaatcc acgcgaaacc gacatacagc gagatacgcg tatccgggtg
    10801 ataaccaatc aacccgataa taaagagcag gaaaatcagc ccgccgatgg tcgttgctac
    10861 cccacccggc actttaaatt tcagcgcctt aacttcttct ggcggcaaac ggcgacggaa
    10921 ggcgatttgc gacagcagga tcataatcca cacccatacc gtggcgaagg ttgccagcga
    10981 agcgatcacc aggaagacgt tttccggcat gatgtagttc agatacaccg caaacagcag
    11041 cgcggtagtc atcaccagca ccgtaaccca cggaataccg cgacgcgacg ttttgctgaa
    11101 aattttcggt gcgctgccct gctctgccat accgtggagc atacggccta cgccaaatac
    11161 gtcactgtta attgccgaca gcgaagcggt cagcacaaca aagttaagaa tgctggcggc
    11221 aaaggtaatg cccatatgct ggaacgtcag tacgaacggg ctaccggcag tgccaacctg
    11281 attccacggg tagatagaca taatgacgaa cagcgtaccg acgtagaaca ccagaatacg
    11341 catcggcacg gagttaatcg cacgcggaat cgatttctca ggatctttcg cttcaccggc
    11401 ggtaatcccg ataatttcga tcccaccgta agcaaacatc accatttgca acgacattac
    11461 catgccaagc cagccgttac tgaagaagcc gccgttgctc cacaggttat gaataccggt
    11521 cggttgcccg ccgttgccaa tcccccagat gatgatgccg aaaccggcga caatcatgat
    11581 gatgatggtg gcgactttaa agaacgagaa ccagaattcc agctcaccga agaccttcac
    11641 gctcatcagg tttacggcgc aaatgatcag caccacgctc agtacccaaa tccagtgcgg
    11701 caccgtcggg aacaagacac ccatatagat accaaaagcg gtcacatcgg cgatggcgac
    11761 aataaggatt tcaaaacagt aggtccatcc ggtaatgtaa cctgccagcg ggccaaggtt
    11821 ttcctgcgca taacgcgaga aagagctggc ggccgggtta tgtaccgaca tttcccccag
    11881 cgcacgcata atgatatacg ccgcgatacc accgataata taggccaaca acacgctcgg
    11941 accggccatt ttgatggcgt ctgccgaacc gtaaaacagc ccggtgccaa ttgctgaacc
    12001 cagtgccata aagcgtatgt ggcgggtact tagcccacgc tttagcttat tcttactttc
    12061 catcaattcc taacccatca acacaataaa aaaccacggg gcatcgcagc cccgtggtta
    12121 aaacaaatgt tcagtgattt agtgagcgct ggaggtcacc tgacgacctg ccgcgcgatc
    12181 ccagataatg gccagaacca ccatcaccac tgttggcatt aaccacgcca gaccttgttc
    12241 ggccagcggt aaacgctgcg cccaggacgg taagatatcg ctgaatgcag atgctttgat
    12301 cccgtcgaga ataccaaaaa gcaggctgat aaacatcggc ggagcaatca cgcgggacga
    12361 attatgccac catgagcgtg taaaacttaa tacaaccagt gcgatacacg gcggataaat
    12421 agcggtcagc accggtacgg agatctggat cagctggctt aagccgaggt tagacaccac
    12481 catcgagaag ccgccgagga taaacaccag cgtacgataa gagagcggta cgtactgggc
    12541 aaagaattct gcacaagcac aggtcaggcc aaccgccgtg accagacagg cgatgaagat
    12601 taacgccgcc agcaggaagc taccgccgcc gccaaaggtg tgctgaacgt aagcatgcag
    12661 aatagcagcg ccgtttgcag actgatcgac cagcgacgcg ctgtctgaac ccagacagaa
    12721 cagcgccagg tacagcaaag tcagaccaac acccgccatc aggccagccc agacggtata
    12781 acgggtcagc agacgcgctt cggtaacgcc acgagaacgc gccgcgttaa caataacgat
    12841 accaaacacc attgcgccca gcgtatccat ggtcagatag ccgttaacga agccgttaga
    12901 aaatgcagcg ttttgataag cctcagtcgc cgtgctgata gaacccgccg gccagacaat
    12961 cgcgacaaca gacaggatga ccagcgcgat aattttcagc ggcgcaagga agttgcccac
    13021 ggtatccagc agcttgcccg gatagagcga aaccagaata acgatagcga aatagaccag
    13081 gctgtagata aacagcggca gcgcggaatc acccgtcagc ggcgcaatcc ccacttcaaa
    13141 ggaaacggta gctgtacgcg gcgtagcgaa aagcggcccc accgccaggt aacaaaccgt
    13201 tgccagcagt acgccagcga ctttaccgat tggcgtgctg aggctgtcaa caccgccgcc
    13261 aacttttgcc agcgccacta ccgttaatac cggcaggcca acggcagtaa tgaggaagcc
    13321 gaatgccgca gtccagacgt gttcgcctgc ctgtaagccg accattggag ggaaaataat
    13381 gttacctgcg ccgacgaaca acgcaaatgt cataaagccc agagcgatga tatcgcgcga
    13441 tcttaattga tgggtcataa atcatactgc ctgtggatgt ggtgttgtaa cgttaaattt
    13501 ttgtgccaat ccctggggat gatacagcgg tatatttaaa caattttagc gggaaatact
    13561 cccaaccaag cgcggcgaag aatagttttt cgatttaccg cgttaatgca gtcagtatga
    13621 cagcacctga agcgcaattt aaacgcttat aacgttttaa agcaaggagg gatctaaaaa
    13681 ccagaggtat atagcgataa gaaatcacca accagtataa aacactattt ccgtgaaaaa
    13741 gacatggcta atcatgcgaa caaaaaatca ccaaccgttg taaaatctaa cgcccgtatt
    13801 ttgactgcaa agaaaataag ccagcatcgg ctggcttatg gcaataaaag atgacaaagg
    13861 cggattaatc gctgtttttg gcaattaaac gttccgggat aacaaaactg aaacgtgttc
    13921 cttttcctac tgtactctca atattcaggc gactttcgtg atgattcacc gcatgtttca
    13981 cgatcgctaa ccctaatccg ctaccgccgg tttgccggga acgcgcttta tcaacgcgat
    14041 aaaaacgctc ggtcaggcgc ggaatatgct ccggtgcaat gcccggtccg ttatcttcaa
    14101 cgctaaattc ggcaccgtgc ggcactcgct gccagcgtac ggtgatatgc gtgccttccg
    14161 gcgtatgatt cacggcgtta tagaccaggt tcgaaatcgc actgcgtagc tgatcttcgt
    14221 tgccagacac cttgaggccg ttatctatct caaaggtaaa tgtctgtttt ttctgactca
    14281 gagtctgagc ctcgcgctca acaacgcgca gcatcatcgg cacatcaacc ttttcattga
    14341 gcaattgcgt cggcgcggct tcaattttcg acagcgttag caactgcttc accagccctt
    14401 ccatccgctg ggtctgctcg cgcatggtgt gcaacgcttt ttcgcgtacc gcgccttcca
    14461 gcggctgctc atccatcatc tccaggtaac cctgtaacac ggtcaatggc gtacgtaact
    14521 catggctcac gttggcaaaa aagttacgcc gcgccccttc cagttgatgc atttgcgtga
    14581 catcacgcgc caccatcagc aactgtttgt gggtataagg catgacgcga atttccagat
    14641 gccgcccggt gttgagcacc agattgagcg ggcgagaaaa atcacgcgtt ttcagatatt
    14701 gcgtaaactc cgggtaacgc agtaggttaa ggatgttctg cccgttatct tccggccagc
    14761 gcaaaccaag aatttgttgc gccagaccgt tacaccagaa aataccgccc tcttccgtgg
    14821 tcagcaccac cgcgtcgggc agcgactccg cgccgctacg aaagcgttta atcagattgc
    14881 ccagttcacg gcggcgtttt ttatttcgca gctgcatctg gtgtaagccg tatagtagcg
    14941 gttcccagct accacgcccc ggtggcgggg tcatactgcg atccacccac agccaccatg
    15001 aaaggcgcaa taaattccag aaatgccaga taagcagtcc tgttaccgat gccagcaaaa
    15061 accagggcag gtaaccaaaa aatgcaccca ggatgaaagc cgggaggcag caaagtagca
    15121 gctccagcac cagccttttc cacgacagcc gttccagcac gtaagatact ccagttaaga
    15181 aatcataagc cctgctctgc gtccgatgag caaggcgtta aaagcgggtt gaaaaacgat
    15241 atcctgtacc gcgcacggtc tgcaccatgc ggtcatgccc gccgggctcc agtgctttac
    15301 gcaggcgacg aatgtggaca tcgaccgtgc ggtcttccac atacacgtta gttccccaga
    15361 cgtggtttaa cagctgctcg cggctgtaca cgcgctcagg atgcgtcata aaaaagtgca
    15421 gcagtttaaa ttctgtcggc cccatctcca gcggctcttc gcccgccatc actcggtgag
    15481 atgtcgggtc gagacttaat ccctgcatct taatcacctc ttccaccgcc attggcgaaa
    15541 tacggcgcat taccgctttg attcgcgcca ccagctcctt cggcgaaaat ggcttggtga
    15601 tatagtcatc cgcgccggtt tcaaggccgc gcacgcgatc ttcttcttcc cctctggcgg
    15661 tcaacatcac cactggaata tcccgggtca tcgactcgcg cttgaggtgt ttgatgaact
    15721 ggataccgga gccgccaggt aacatccagt caaggagaat taaatccggc tagggttcat
    15781 tcagttgatt cacagcactg tcataatctt ccgcttcgac cggctgaaag ccattttgtt
    15841 cgagcacgaa gcagaccatt tcgcgaattg gagcttcatc ttctacgacc agaatacgtc
    15901 tcgccatgat ttgccctgtt gtaataaata ggttgcgatc attaatgcga cgtcattatg
    15961 cgtcagattt atgacagatt tatgaaaagc tcgtcgcaca tatcttcagg ttattgattt
    16021 ccgtggcgca gaaaaaagca aatggcacat ctgtttgggt ataatcgcga ccatgctttt
    16081 tcgccaggga accgttatgc gcatccttca cacctcagac tggcatctcg gccagaactt
    16141 ctacagtaaa agccgcgaag ctgaacatca ggcttttctt gactggctgc tggagacagc
    16201 acaaacccat cagatggatg cgattattgt tgctggtgat gttttcgata ccggctcgcc
    16261 gcccagttac gcccgcacgt tatacaaccg ttttgttgtc aatttacagc aaactggctg
    16321 tcatctggtg gtactggcag gaaaccatga ctcggtcgcc acgctgaatg aatcgcgcga
    16381 tatcatggcg ttcctcaata caaccgtggt cgccagcgcc ggacatgcgc cgcaaatctt
    16441 gcctcgtcgc gacgggacgc caggcgcagt gctgtgcccc attccgtttt tacgcccgcg
    16501 tgacattatt tccagccagg cggggcttaa cggtattgaa aaacagcagc atttactggc
    16561 agcgattacc gattattacc aacaacacta tgccgatgcc tgcaaactgc gcggcgatca
    16621 gcctctgccc atcatcgcca cgggacattt aacgaccgtg ggttccagta aaagtgacgc
    16681 cgtgcgtgac atttatattg gcacgctgga cgcgtttccg gcacaaaact ttccaccagc
    16741 cgactacatc gcgttcgggc atattcaccg cgcacagatt attggcggca tggaacatgt
    16801 tcgctattgc ggttccccca ttccactgag ttttgatgaa tgcggtaaga gtaaatatgt
    16861 ccatctggtg acattttcaa acggcaaatt agagagcgtg gaaaacctga acgtaccggt
    16921 aactcaaccc atggcggtgc tgaaaggcga tctggcgtcg attaccgcac agctggaaca
    16981 gtggcgcgat gtatcgcagg agccacctgt ctggctggat atcgaaatca ctactgatga
    17041 gtatctgcat gatattcagc gcaaaatcca ggcattaacc gaatcattgc ctgtcgaagt
    17101 attgctggta cgtcggagtc gtgaacagcg cgagcgtgtg ttagccagcc aacagcgtga
    17161 aaccctcagc gaactcagcg tcgaagaggt gttcaatcgc cgtctggcac tggaagaact
    17221 ggatgaatcg cagcagcaac gtctgcagca tcttttcacc acgacgttgc gtaccctcgc
    17281 cggagaacac gaagcatgaa aattctcagc ctgcgcctga aaaacctgaa ctcattaaaa
    17341 ggcgaatgga agattgattt cacccgcgag ccgttcgcca gcaacgggct gtttgccatt
    17401 accggcccaa ccggtgcggg gaaaaccacc ctgctggacg ccatttgtct ggcgctgtat
    17461 cacgaaactc cgcgtctctc taacgtttca caatcgcaaa atgatctcat gacccgcgat
    17521 accgctgaat gtctggcgga ggtggagttt gaagtgaaag gtgaagcgta tcgcgcgttc
    17581 tggagccaga atcgggcgcg taaccagccg gacggtaatt tgcaggtgcc tcgcgtagag
    17641 ctggcgcgct gcgccgacgg caaaattctc gccgacaaag tgaaagataa gctggaactg
    17701 acagcgacgt taaccgggct ggattacggg cgcttcaccc gttcgatgct gctttcgcag
    17761 gggcaatttg ctgccttcct gaatgccaaa cccaaagaac gcgcggaatt gctcgaggag
    17821 ttaaccggca ctgaaatcta cgggcaaatc tcggcgatgg tttttgagca gcacaaatcg
    17881 gcccgcactg agctggagaa gctgcaagcg caggccagcg gcgtcacgtt gctcacgccg
    17941 gaacaagtgc aatcgctgac agcgagtttg caggtactta ctgacgaaga aaaacagtta
    18001 attaccgcgc agcagcaaga acaacaatcg ctaaactggt taacgcgtca ggacgaattg
    18061 cagcaagaag ccagccgccg tcagcaggcc ttgcaacagg cgttagccga agaagaaaaa
    18121 gcgcaacctc aactggcggc gcttagtctg gcacaaccgg cacgaaatct tcgtccacac
    18181 tgggaacgca tcgcagaaca cagcgcggcg ctggcgcata ttcgccagca gattgaagaa
    18241 gtaaatactc gcttacagag cacaatggcg cttcgcgcga gcattcgcca ccacgcggcg
    18301 aagcagtcag cagaattaca gcagcagcaa caaagcctga atacctggtt acaggaacac
    18361 gaccgcttcc gtcagtggaa caacgaactg gcgggttggc gtgcgcagtt ctcccaacaa
    18421 accagcgatc gcgagcatct gcggcaatgg cagcaacagt taacccatgc tgagcaaaaa
    18481 cttaatgcgc ttgcggcgat cacgttgacg ttaaccgccg atgaagttgc taccgccctg
    18541 gcgcaacatg ccgagcaacg cccactgcgt cagcgcctgg tcgcgctgca tgggcagatt
    18601 gttccccaac aaaaacgtct ggcgcagtta atcgtcacta tccagaatgt cactctggaa
    18661 cagacgcaac gtaatgtcgc acttaacgaa atgcgccagc gttataaaga aaagacgcag
    18721 caacttgccg atgtgaaaac catttgcgag caggaagcgc gcatcaaaac gctggaagcc
    18781 cagcgtgcac agttacaggc gggtcagcct tgtccacttt gtggttccac cagccacccg
    18841 gcggtcgagg cgtatcaggc gctggagcct ggtgttaatc agtctcgatt actggcgctg
    18901 gaaaacgaag ttaaaaagct cggcgaagaa ggtgcggcgc tgcgcgggca actggatgca
    18961 ttaacgaagc agcttcagcg cgatgaaaac gaagcgcaaa gcctccgaca agatgagcaa
    19021 gcacttactc aacaatggca agccgtcacg gccagcctca atatcacctt gcagccacag
    19081 gacgatattc aaccgtggct ggatgcacaa gatgagcacg aacgccagct gcggttactc
    19141 agccaacgac atgaattaca agggcagatt gccgcgcata atcagcaaat tatccagtat
    19201 caacagcaaa ttgaacaacg tcagcagcag ctcttaacgg cattggcggg ttatgcactg
    19261 acattgccac aggaagatga agaagagagc tggttggcga cacgtcagca agaagcgcag
    19321 agctggcagc aacgccagaa cgaattaacc gcgctgcaaa accgtattca gcagctgaca
    19381 ccgattctgg aaacgttgcc gcaaagtgat gatctcccgc acagcgaaga aactgtggcg
    19441 ctggataact ggcggcaggt tcatgaacaa tgtctcgcat tacacagcca gcagcagacg
    19501 ttacagcaac aggatgttct ggcggcgcaa agtctgcaaa aagcccaagc gcagtttgac
    19561 accgcgctac aggccagcgt ctttgacgat cagcaggcgt tccttgcggc gctaatggat
    19621 gaacaaacac taacgcagct ggaacagctc aagcagaatc tggaaaacca gcgccgtcag
    19681 gcgcaaacgc tggtcactca gacagcagaa acgctggcac agcatcaaca acaccgacct
    19741 gacgggttgg ctctcactgt gacggtggag cagattcagc aagagttagc gcaaactcac
    19801 caaaagttgc gtgaaaacac cacgagtcaa ggcgagattc gccagcagct gaagcaggat
    19861 gcagataacc gtcagcaaca acaaacctta atgcagcaaa ttgctcaaat gacgcagcag
    19921 gttgaggact ggggatatct gaattcgcta ataggttcca aagagggcga taaattccgc
    19981 aagtttgccc aggggctgac gctggataat ttagtccatc tcgctaatca gcaacttacc
    20041 cggctgcacg ggcgctatct gttacagcgc aaagccagcg aggcgctgga agtcgaggtt
    20101 gttgatacct ggcaggcaga tgcggtacgc gatacccgta ccctttccgg cggcgaaagt
    20161 ttcctcgtta gtctggcgct ggcgctggcg ctttcggatc tggtcagcca taaaacacgt
    20221 attgactcgc tgttccttga tgaaggtttt ggcacgctgg atagcgaaac gctggatacc
    20281 gcccttgatg cgctggatgc cctgaacgcc agtggcaaaa ccatcggtgt gattagccac
    20341 gtagaagcga tgaaagagcg tattccggtg cagatcaaag tgaaaaagat caacggcctg
    20401 ggctacagca aactggaaag tacgtttgca gtgaaataac tattcagcag gataatgaat
    20461 acagaggggt gaattatctc ttggccttgc tggtcgttat cctgcaagct atcactttat
    20521 tggctacggt gattggtagc cgttctggtg gttgtgatgg tggtatgaaa aaagtcattt
    20581 tatctttggc tctgggcacg tttggtttgg ggatggccga atttggcatt atgagcgtgc
    20641 tcacggagct ggcgcataac gtaggaattt cgattcctgc tgccgggcat atgatctcgt
    20701 attatgcact ggtggtggtg gtcggtgcgc caatcatcgc actcttttcc agccgctact
    20761 cactcaaaca tatcttgttg tttctggtgg cgttgtgcgt cattggcaac gccatgttca
    20821 cgctctcttc gtcttacctg atgctcgcca ttggtcggct ggtatccggc tttccgcatg
    20881 gcgcattttt tggcgtcgga gcgatcgtgt tatcaaaaat tatcaaaccc ggaaaagtca
    20941 ccgccgccgt ggcggggatg gtttccggga tgacagtcgc caatttgctg ggcattccgc
    21001 tgggaacgta tttaagtcag gaatgctggc gttacacctt tttattgatc gctgttttta
    21061 atattgcggt gatggcatcg gtctattttt gggtgccaga tattcgcgac gaggcgaaag
    21121 gaaatctgcg cgaacaattt cactttttgc gcagcccggc cccgtggtta attttcgccg
    21181 ccacgatgtt tggcaacgca ggtgtgtttg cctggttcag ctacgtaaag ccatacatga
    21241 tgtttatttc cggtttttcg gaaacggcga tgacctttat tatgatgtta gttgggctag
    21301 ggatggtgct gggaaatatg ctaagtggca ggatttcagg acgttattca ccactgcgca
    21361 ttgcagcagt gactgacttt atcattgtac tggcactgct gatgctcttt ttctgcggcg
    21421 gcatgaaaac aacgtcgctt atttttgctt ttatttgttg cgcgggatta tttgcccttt
    21481 cagcacctct gcaaatattg ttactgcaaa atgccaaagg aggagagtta ttaggtggcg
    21541 caggtgggca aatagcgttt aacctcggta gcgccgtcgg cgcatattgc ggaggtatga
    21601 tgctgacgct ggggctggca tataattacg tggcgctgcc tgccgccctg ctttcgtttg
    21661 ctgcgatgtc atcgttgctg ctgtatggtc gctataagcg ccagcaagcg gcggatagtc
    21721 cggtgctggc gaaaccactg gggtagatta ctgtcacggt gcgcgttgct tattgccgga
    21781 tgcggcgtga acgccttatc cgccctacgc ggttctggca cattttgtag gcctgataag
    21841 acgcggcaag cgtcgcatca ggcatgatgc gccaattgcc tacgtttttt actcttgtgg
    21901 ccataaccac gcagcgccgc gtacgccgct ggaatcaccg tgcttcgcct tactcaccgg
    21961 cgtttcacat tcgccgccga agacaaattg tttaatcaac tgcccaaccg tttgatataa
    22021 acggtctaca ttgctcatcc cgccccccag gacaatcaca tccggatcga gaatattcac
    22081 gacatgtgcc agcgattttg ccagccgcag ctcgtagcga cgcaatgcca gttccgctac
    22141 cggatcgctt tcttcaacca ggcggataat ttcactgcct ttcagcgcat gtccgctcaa
    22201 acgacgataa tccgtcgcga atcccgtgcc cgaaataaag gtttcaatac aaccttgttt
    22261 accgcaataa caagggactt cctcgcgata acgcagttcg tcttcgtcca tccacggtag
    22321 cggattgtgt ccccact
//
LOCUS       DADLCQ010000059        22287 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000059 DADLCQ010000000
VERSION     DADLCQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.059"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1205
                     /gene="cysJ"
                     /locus_tag="JQG95_002169"
     CDS             <1..1205
                     /gene="cysJ"
                     /locus_tag="JQG95_002169"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY5888712.1"
                     /translation="RVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASLSV
                     NQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDEP
                     VTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTL
                     IVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGRAR
                     AGGASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRA
                     ADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLR
                     EQGAELWRWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVE
                     RRYQRDVY"
     gene            1205..2917
                     /gene="cysI"
                     /locus_tag="JQG95_002170"
     CDS             1205..2917
                     /gene="cysI"
                     /locus_tag="JQG95_002170"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY5888713.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            2991..3725
                     /gene="cysH"
                     /locus_tag="JQG95_002171"
     CDS             2991..3725
                     /gene="cysH"
                     /locus_tag="JQG95_002171"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY5888714.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            3989..4141
                     /locus_tag="JQG95_002172"
     CDS             3989..4141
                     /locus_tag="JQG95_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5888715.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            4335..5486
                     /locus_tag="JQG95_002173"
     CDS             4335..5486
                     /locus_tag="JQG95_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY5888716.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGIDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(5572..6609)
                     /gene="iap"
                     /locus_tag="JQG95_002174"
     CDS             complement(5572..6609)
                     /gene="iap"
                     /locus_tag="JQG95_002174"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY5888717.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            6861..7769
                     /gene="cysD"
                     /locus_tag="JQG95_002175"
     CDS             6861..7769
                     /gene="cysD"
                     /locus_tag="JQG95_002175"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY5888718.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            7771..9198
                     /gene="cysN"
                     /locus_tag="JQG95_002176"
     CDS             7771..9198
                     /gene="cysN"
                     /locus_tag="JQG95_002176"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY5888719.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPDNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            9198..9803
                     /gene="cysC"
                     /locus_tag="JQG95_002177"
     CDS             9198..9803
                     /gene="cysC"
                     /locus_tag="JQG95_002177"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY5888720.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            9853..10176
                     /locus_tag="JQG95_002178"
     CDS             9853..10176
                     /locus_tag="JQG95_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY5888721.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGSLF
                     MWLLG"
     gene            10370..10681
                     /gene="ftsB"
                     /locus_tag="JQG95_002179"
     CDS             10370..10681
                     /gene="ftsB"
                     /locus_tag="JQG95_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY5888722.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            10700..11410
                     /gene="ispD"
                     /locus_tag="JQG95_002180"
     CDS             10700..11410
                     /gene="ispD"
                     /locus_tag="JQG95_002180"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY5888723.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            11410..11889
                     /gene="ispF"
                     /locus_tag="JQG95_002181"
     CDS             11410..11889
                     /gene="ispF"
                     /locus_tag="JQG95_002181"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY5888724.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            11886..12935
                     /gene="truD"
                     /locus_tag="JQG95_002182"
     CDS             11886..12935
                     /gene="truD"
                     /locus_tag="JQG95_002182"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY5888725.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            12916..13677
                     /gene="surE"
                     /locus_tag="JQG95_002183"
     CDS             12916..13677
                     /gene="surE"
                     /locus_tag="JQG95_002183"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY5888726.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            13671..14297
                     /gene="pcm"
                     /locus_tag="JQG95_002184"
     CDS             13671..14297
                     /gene="pcm"
                     /locus_tag="JQG95_002184"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY5888727.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            14437..15576
                     /gene="nlpD"
                     /locus_tag="JQG95_002185"
     CDS             14437..15576
                     /gene="nlpD"
                     /locus_tag="JQG95_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY5888728.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTMSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            15639..16631
                     /gene="rpoS"
                     /locus_tag="JQG95_002186"
     CDS             15639..16631
                     /gene="rpoS"
                     /locus_tag="JQG95_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY5888729.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(16751..17158)
                     /locus_tag="JQG95_002187"
     CDS             complement(16751..17158)
                     /locus_tag="JQG95_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY5888730.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            17305..17898
                     /locus_tag="JQG95_002188"
     CDS             17305..17898
                     /locus_tag="JQG95_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY5888731.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            17898..18845
                     /locus_tag="JQG95_002189"
                     /pseudo
     CDS             17898..18845
                     /locus_tag="JQG95_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(19118..19681)
                     /locus_tag="JQG95_002190"
     CDS             complement(19118..19681)
                     /locus_tag="JQG95_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY5888732.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            19846..20097
                     /locus_tag="JQG95_002191"
     CDS             19846..20097
                     /locus_tag="JQG95_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5888733.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            20099..22195
                     /locus_tag="JQG95_002192"
     CDS             20099..22195
                     /locus_tag="JQG95_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY5888734.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         5526 a         5456 c         6036 g         5269 t
ORIGIN      
        1 cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg caatccgtcg
       61 ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg ccgctggtgg
      121 ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac gttcgccata
      181 tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg ctgggcgtct
      241 ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg ctgacaggcg
      301 atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta cagtggcact
      361 tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt acccgcagtg
      421 aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc gcgacgacgc
      481 tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg ctaattaatc
      541 tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg gaagtcgaga
      601 acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc gcccgtgccg
      661 gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc cgcgtattta
      721 tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg attatgattg
      781 gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc gctgacgaag
      841 cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac ttcctgtatc
      901 aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat ctcgcctggt
      961 cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag ggcgcggagc
     1021 tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct aatcgcatgg
     1081 cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc atggacaccg
     1141 aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag cgagatgtct
     1201 actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga cagacgccga
     1261 gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt taaacgacgg
     1321 tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg gcatgtatca
     1381 gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc cgcgccacgc
     1441 gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt ggcaggcgat
     1501 cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca accgccagac
     1561 gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga tgctgcactc
     1621 ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac tctgcacctc
     1681 gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga agatttctga
     1741 acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg aaaaagtcgc
     1801 caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt tcaaaaccac
     1861 ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga acttcgtggc
     1921 gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg ggctttctat
     1981 cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct atctgccgct
     2041 agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact ggggtaaccg
     2101 aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg ttgagacgtt
     2161 taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc cgtatgaatt
     2221 tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact ggcacctgac
     2281 gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga aaactggcct
     2341 gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc agaatctgat
     2401 catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca aagagagcgg
     2461 gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt cattcccgac
     2521 ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg acaacatcga
     2581 taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg taacaggctg
     2641 cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ttggtgggta aagcaccggg
     2701 tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac ggatgtataa
     2761 agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag ggcgctgggc
     2821 gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg gcatcattcg
     2881 cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt cttgtaggcc
     2941 tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct atgtccaaac
     3001 tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg ctggcagaaa
     3061 ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg ctggataatc
     3121 tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg agcctgcatc
     3181 tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc tatctgttcc
     3241 cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac ctgaaagtgt
     3301 accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg tgggaacagg
     3361 gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg aaccgggcgc
     3421 tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa tccggcagcc
     3481 gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg ccgattatcg
     3541 actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa tatcacccat
     3601 tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa tgggaacccg
     3661 gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga ttacacgaag
     3721 ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc gtttttattt
     3781 tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac aggtatagaa
     3841 tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca gatggacaaa
     3901 agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca attcggatgt
     3961 tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg tggcaatcat
     4021 cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt tatgtgagtt
     4081 gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat cgaagaagta
     4141 gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc tcctgcaccc
     4201 tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat tatgaatttc
     4261 aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata tatatcatag
     4321 atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa aatattgtga
     4381 ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc gacgactatc
     4441 accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg gttaaaagaa
     4501 attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag gaacaggcag
     4561 ctcaattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc cgctcttttc
     4621 aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa aattacgaaa
     4681 aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt gaatgtcagg
     4741 agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata gaaatgtgga
     4801 tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa ttggataatt
     4861 ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag ccactgtttc
     4921 cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt ataaaacaac
     4981 tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg tcaacgcgat
     5041 tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag gctttagttc
     5101 aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct ttcaccggaa
     5161 ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag gtgctggatc
     5221 tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc ggcgcaggta
     5281 aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac gctcaggggc
     5341 tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga ctggtcaaaa
     5401 cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata aaccggcgca
     5461 atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat gggtatcact
     5521 tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc atcaagacgc
     5581 cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc agcgacgttc
     5641 tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta cgtcatgcca
     5701 gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat ccttatttcc
     5761 aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt cgaatatttc
     5821 tgcgtcatta caacacccag tgcctttcgg ataattttta ttcaaacccg gattggtcgt
     5881 tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta atttccttac
     5941 tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa ttaagttatc
     6001 gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct tgagtaaatt
     6061 ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa taccatactc
     6121 ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac ctgcggcgtt
     6181 atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag catcgctcag
     6241 cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt tgccttcatg
     6301 agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat cgcgggcggt
     6361 ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt gctgaaactg
     6421 ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc gtcccggaaa
     6481 gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag gtgacgaggc
     6541 gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt ggcgcaatgc
     6601 ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata gtatgaaact
     6661 gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt gccttaagca
     6721 ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca ttctaattgg
     6781 taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca agaaaatagc
     6841 ggtattgcaa aggaacggtt atggatcaaa aacgacttac tcacctgcgg caactggagg
     6901 cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg gtgatgctct
     6961 actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg ttttatccag
     7021 gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc gagatgtatg
     7081 agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat aaaaacccgg
     7141 aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat accgatatta
     7201 tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc gccttcggtg
     7261 gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct ttccgtgacc
     7321 gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac tacaacgggc
     7381 aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc gagcaggata
     7441 tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc gctgcggaac
     7501 gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt atcgacctgc
     7561 aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc tgctggccgc
     7621 tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa gagatgctgg
     7681 tttccaccac cagtgaacgt caggggcgcg tgattgaccg cgaccaggcg ggatccatgg
     7741 agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca acaaatcgcc
     7801 aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct gctgcgtttt
     7861 ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct gctgcacgat
     7921 acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa gcgtcacggc
     7981 acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc tgagcgcgaa
     8041 cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg taaatttatt
     8101 atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg cgcatcgaca
     8161 tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca aacccgtcgt
     8221 cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat caacaaaatg
     8281 gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta tctgaccttt
     8341 gccgggcagc tgccggataa tctggatatc cgctttgtgc cgctctccgc actggaaggt
     8401 gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac actgctcgaa
     8461 gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg cttcccggtg
     8521 cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct ggcatccggt
     8581 cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc aaacgtcgcg
     8641 cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc gatcaccctg
     8701 gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc agacgaagcg
     8761 ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca gccgctttcc
     8821 ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg tgttgatggc
     8881 attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa cctgccgctg
     8941 aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga tcgctatcag
     9001 caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt gaccgtgggt
     9061 gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga attcagtgca
     9121 ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg cgcgcgcgat
     9181 ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat agtcatccgg
     9241 tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg tggtttaccg
     9301 gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg ttacataaac
     9361 tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta tgcagcgatc
     9421 tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa gtggcgaatt
     9481 tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac cgcgccgaac
     9541 gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt gtcgatacgc
     9601 cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg cgtgccggtg
     9661 aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg gcagaaattc
     9721 atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat ctgttgagac
     9781 agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt cgtcaaagta
     9841 acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga cagccttact
     9901 gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat ctcctggtta
     9961 tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt ctttctctac
    10021 acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat tgcgctgcat
    10081 tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac agtaggcatt
    10141 atgtttggct cactgtttat gtggctactg ggctaatttg tactttcttg tctcctctgt
    10201 tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg gtatacgtaa
    10261 caatggacaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca gaggatgtag
    10321 agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga tgggtaaact
    10381 aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt tcggtaagaa
    10441 cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag ctacaaacgc
    10501 gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca atggcggcca
    10561 ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg gcgaaacttt
    10621 ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa acaatcgata
    10681 aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg tggttccggc
    10741 ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct caatcggtaa
    10801 tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg tgacacgtgt
    10861 cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg cgaatcatcc
    10921 gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg caggtctgaa
    10981 agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt gtttgcatca
    11041 ggatgacctc gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag ggatcctcgc
    11101 cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca ttgctcatac
    11161 cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc gtgagctgtt
    11221 acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg aagcctcggc
    11281 gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata acattaaagt
    11341 cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca tccatcagga
    11401 gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg tgaaggccca
    11461 attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca ttctgatggc
    11521 gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct gggggatatc
    11581 ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg cgagctgcta
    11641 cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt cgatgtcact
    11701 atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt gtttattgcc
    11761 gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac ggaaaaactt
    11821 ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact cattaaggca
    11881 acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa ggcaccgggc
    11941 tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt gagcctgatg
    12001 gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc cgttttgtgg
    12061 cggatgcact ggcgaaattc ctgaaaattc attcccgtga agtcagcttc gctgggcaaa
    12121 aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc aaggaaatgc
    12181 ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg cggcacaagc
    12241 gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg cgcgaagtga
    12301 gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt gtaccgaact
    12361 acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg ctgcgctggg
    12421 cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg tcggcagccc
    12481 gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac gttaatcaag
    12541 ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta gcaaccaccg
    12601 aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata accgccgcat
    12661 tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa caagcagctg
    12721 tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa gccgcgcgca
    12781 gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac gtcaccgtag
    12841 agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg gaacttatca
    12901 acacaacagg tgattatgcg catattgctg agtaatgatg acggggtaca tgcacccggt
    12961 atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt cgcccccgat
    13021 cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg cacgtttacc
    13081 tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt ctatcttggc
    13141 gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa cgccgggccg
    13201 aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga aggccgtcat
    13261 ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga cactgccgcg
    13321 gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac cggacgtatt
    13381 cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg cgtgacgcgc
    13441 tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg cggcaatacg
    13501 ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac cgattttgct
    13561 gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac tgcgcatagc
    13621 gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca atggtaagca
    13681 gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat gagcaggtgc
    13741 tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt gaacaaaaag
    13801 cttgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag ccgtatatgg
    13861 tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg gaaattggca
    13921 ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt tgctcggttg
    13981 aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt gatttacata
    14041 atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg ccgtttgacg
    14101 ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg cagctggacg
    14161 aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa cgggtgcgtc
    14221 gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc cctttagtga
    14281 agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta ttttgccgta
    14341 ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca cggtaattat
    14401 gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag cccaaaattc
    14461 accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg ctgttctgac
    14521 acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc aaatactaat
    14581 tctggtatgt tgattacgcc gccgccgaaa atggggacga tgtctacagc gcagcaaccg
    14641 caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca aatccagcca
    14701 gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt ctataaccgt
    14761 cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt gaaaaaaggc
    14821 gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct tgctcagcgc
    14881 aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt gggtaatgct
    14941 tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc agagcaagga
    15001 gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc gacaattacg
    15061 tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa caagccaact
    15121 gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga gccgactgtc
    15181 agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga gggcaaagtg
    15241 atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc aggcagcaaa
    15301 ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa cgcgctgcgc
    15361 ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc ctacgcccat
    15421 aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa aatagcaacc
    15481 atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta caaggggaaa
    15541 tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag gcttttgctt
    15601 gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat acgctgaaag
    15661 ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt tttgacgaaa
    15721 aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa ctgttatcgc
    15781 agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag attggttatt
    15841 caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg cgtggagatg
    15901 tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa attgcccgcc
    15961 gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac ttggggctga
    16021 tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca tacgcaacct
    16081 ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact attcgtttgc
    16141 cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag ttgtcccata
    16201 agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag ccagttgatg
    16261 acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc ccgctgggtg
    16321 gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac ggtccggaag
    16381 ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc gagctgaacg
    16441 ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa gcggcaacac
    16501 tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag attcaggttg
    16561 aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc gaagcgctgt
    16621 tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg gcctttttct
    16681 tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt cctacccggc
    16741 aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc tcctgctctt
    16801 ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt atcgccgcag
    16861 caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg tcagcgacgt
    16921 catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct gctagcgttg
    16981 ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt ttatcagcaa
    17041 tggcacgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc tgccagcgag
    17101 ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt aacgccatgt
    17161 aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga agaagatgtt
    17221 gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat aacgttcgta
    17281 tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg ggctaccggt
    17341 gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt cgagactcat
    17401 ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta cagcgctcgc
    17461 gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc gatcatctca
    17521 tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa aacgctcgcc
    17581 ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt cgtgctcaaa
    17641 gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat ccatctcgaa
    17701 aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc tgccttttat
    17761 aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt gctggatcaa
    17821 tttggcctcg aacatcccca cgccaggcgc tggcaaggat taccgcaggc ccggaatttt
    17881 tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag gcgcttgatg
    17941 accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat ctggcagcag
    18001 cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt gataatattc
    18061 gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag aaccacgcga
    18121 tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag tttatccgcc
    18181 gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg gcgcagaaca
    18241 ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc ctgaatgatg
    18301 gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg ctcgacccgg
    18361 ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc aagcgtaagc
    18421 tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa gcagaagagc
    18481 gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc acgctgatgg
    18541 gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg ctgcgtgaaa
    18601 gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg ggttcagaag
    18661 tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg ttcggtgagt
    18721 ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat aaagtctctt
    18781 atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc gaaatcgact
    18841 acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
    18901 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
    18961 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
    19021 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
    19081 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
    19141 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
    19201 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
    19261 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
    19321 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
    19381 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
    19441 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
    19501 gtgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
    19561 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
    19621 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
    19681 ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa taaaccataa
    19741 aaacaccaaa gaacccttta cagggaatct ttggtatctt aatctttatc catacggata
    19801 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
    19861 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
    19921 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
    19981 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
    20041 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc gtcgtgatat
    20101 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
    20161 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
    20221 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
    20281 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
    20341 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
    20401 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gctgggtatc
    20461 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaaccccgg
    20521 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
    20581 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
    20641 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
    20701 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
    20761 tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag cctacgagga
    20821 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
    20881 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga aacaaaaagg
    20941 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
    21001 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
    21061 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
    21121 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
    21181 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
    21241 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
    21301 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
    21361 gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg gtgctgaccg
    21421 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
    21481 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
    21541 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
    21601 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gccaggcaga
    21661 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
    21721 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
    21781 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
    21841 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
    21901 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
    21961 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
    22021 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
    22081 ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac gcgatttccg
    22141 gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg cctgaataaa
    22201 aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt gaaataaccg
    22261 aggaaatact atgactgata ttgatga
//
LOCUS       DADLCQ010000060        22242 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000060 DADLCQ010000000
VERSION     DADLCQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.060"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..495
                     /locus_tag="JQG95_002193"
     CDS             <1..495
                     /locus_tag="JQG95_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888735.1"
                     /translation="WKEKALTQRGLLVQAGDQSEQIFTRWRAGAYNAWSLPGRCFIVL
                     EELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTRHYYHQWFASSTV
                     PTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVGAMVEEIFS
                     VNLA"
     gene            759..2261
                     /gene="proP"
                     /locus_tag="JQG95_002194"
     CDS             759..2261
                     /gene="proP"
                     /locus_tag="JQG95_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY5888736.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            2373..2462
                     /gene="pmrR"
                     /locus_tag="JQG95_002195"
     CDS             2373..2462
                     /gene="pmrR"
                     /locus_tag="JQG95_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY5888737.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(2438..3538)
                     /gene="pmrB"
                     /locus_tag="JQG95_002196"
     CDS             complement(2438..3538)
                     /gene="pmrB"
                     /locus_tag="JQG95_002196"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY5888738.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3539..4207)
                     /gene="basR"
                     /locus_tag="JQG95_002197"
     CDS             complement(3539..4207)
                     /gene="basR"
                     /locus_tag="JQG95_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY5888739.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(4204..5847)
                     /gene="eptA"
                     /locus_tag="JQG95_002198"
     CDS             complement(4204..5847)
                     /gene="eptA"
                     /locus_tag="JQG95_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY5888740.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRATNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(5951..7288)
                     /gene="adiC"
                     /locus_tag="JQG95_002199"
     CDS             complement(5951..7288)
                     /gene="adiC"
                     /locus_tag="JQG95_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY5888741.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(7425..8186)
                     /locus_tag="JQG95_002200"
     CDS             complement(7425..8186)
                     /locus_tag="JQG95_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5888742.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(8511..10778)
                     /gene="adiA"
                     /locus_tag="JQG95_002201"
     CDS             complement(8511..10778)
                     /gene="adiA"
                     /locus_tag="JQG95_002201"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY5888743.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(10977..11885)
                     /gene="melR"
                     /locus_tag="JQG95_002202"
     CDS             complement(10977..11885)
                     /gene="melR"
                     /locus_tag="JQG95_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY5888744.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            12168..13511
                     /gene="melA"
                     /locus_tag="JQG95_002203"
     CDS             12168..13511
                     /gene="melA"
                     /locus_tag="JQG95_002203"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY5888745.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPS"
     gene            13638..15047
                     /gene="melB"
                     /locus_tag="JQG95_002204"
     CDS             13638..15047
                     /gene="melB"
                     /locus_tag="JQG95_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY5888746.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(15186..15815)
                     /locus_tag="JQG95_002205"
     CDS             complement(15186..15815)
                     /locus_tag="JQG95_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY5888747.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(15938..17584)
                     /locus_tag="JQG95_002206"
     CDS             complement(15938..17584)
                     /locus_tag="JQG95_002206"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY5888748.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(17662..19002)
                     /locus_tag="JQG95_002207"
     CDS             complement(17662..19002)
                     /locus_tag="JQG95_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY5888749.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(19573..20292)
                     /gene="dcuR"
                     /locus_tag="JQG95_002208"
     CDS             complement(19573..20292)
                     /gene="dcuR"
                     /locus_tag="JQG95_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY5888750.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(20289..21920)
                     /gene="dcuS"
                     /locus_tag="JQG95_002209"
     CDS             complement(20289..21920)
                     /gene="dcuS"
                     /locus_tag="JQG95_002209"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY5888751.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            22101..>22242
                     /gene="yjdI"
                     /locus_tag="JQG95_002210"
     CDS             22101..>22242
                     /gene="yjdI"
                     /locus_tag="JQG95_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY5888752.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         5348 a         5395 c         5759 g         5740 t
ORIGIN      
        1 tggaaggaaa aggcgttaac gcaacgaggc ctactggtgc aggcaggtga tcagagcgag
       61 caaattttta cccgctggcg tgctggggcg tataacgcct ggtcgttgcc tgggcgctgt
      121 tttatcgttc tggaggagtt gcgctggggt gcatttggcg atgcctgccg tctgggaagc
      181 ccgcaagcgg tggtgttgtt gctgggtgat ttgcgcgaga aagcgacaca acatctggca
      241 gagagtatca atgcggcacc gactacgcgt cactattacc atcagtggtt tgcctcttcg
      301 accgttccga cgggcgggga tcatgctgat tttttaagtt ggctgggaaa gtggaccacg
      361 gcagataaac aacccgtttg ctggtcagtg acccaacgct ggcaaactgt cgcgctgggg
      421 atgccacgac tctgttcagc gcagcgtttg gtgggggcaa tggtcgagga aatcttctct
      481 gtaaatttgg cgtaaataat cagttacatc aatgagtcct aaacgaaatc catgtgtgaa
      541 gttgatcaca aatttaaaca ctggtagggt aaaaaggtca ttaactgccc aattcaggcg
      601 tcaactggtt tgattgtaca ttccttaacc ggagggtgta agcaaacccg ctacgcttgt
      661 tacagagatt gcatcctgca attcccgctc cccttttgcg gccgtcgcgc tgatttttct
      721 ggcgtttgcg gaaatgggcc aactctgcga ggaaagctat gctgaaaagg aaaaaagtaa
      781 aaccgattac ccttcgtgat gtcaccatta ttgatgacgg taaactgcgt aaagccatta
      841 ccgcagcatc actgggtaat gcaatggaat ggtttgattt tggtgtttat ggttttgttg
      901 cttacgcatt aggtaaagtt tttttcccgg gggctgaccc cagcgtgcag atggttgctg
      961 cacttgccac tttctccgtt ccctttctga ttcgaccgct tggcggactc ttctttggta
     1021 tgttgggcga taaatatggt cgccagaaga tcctcgctat cactattgtg attatgtcga
     1081 tcagtacgtt ctgtattggc ttaataccgt cctacgacac gattggtatt tgggcaccga
     1141 ttctgctgtt gatctgtaag atggcacaag gtttctcggt cggtggtgaa tataccgggg
     1201 cgtcgatatt tgttgcggaa tactcccctg accgtaaacg tggctttatg ggcagctggc
     1261 tggacttcgg ttctattgcc gggtttgtgc tgggtgcggg cgtggtggtg ttaatttcga
     1321 ccattgtcgg cgaagcgaac ttcctcgact ggggctggcg tattccgttc tttattgctc
     1381 tgccgttagg gattatcggg ctttacctgc gccatgcgct ggaagaaact ccggcgttcc
     1441 agcagcatgt tgataaactg gaacagggcg accgcgaagg tttgcaggat ggcccgaaag
     1501 tctcgtttaa agagattgcc actaaatact ggcgcagcct gttgacatgt attggtctgg
     1561 taattgccac caacgtgact tactacatgt tgctgaccta tatgccgagt tatttgtcgc
     1621 ataacctgca ttactccgaa gaccacgggg tgctgattat tatcgccatt atgatcggta
     1681 tgctgtttgt ccagccggtg atgggcttgc tgagtgaccg ttttggccgt cgtccgtttg
     1741 tgctacttgg tagtgttgca ctgtttgtgt tggcgatccc ggcgtttatt ctgattaaca
     1801 gtaacgtcat cggcctgatt tttgccgggt tactgatgct ggcggtgatc cttaactgct
     1861 ttatgggcgt tatggcttct accttgccag cgatgttccc gacgcatatc cgttacagcg
     1921 cgctggcggc ggcatttaat atttcggtgc tggttgccgg tctgacgcca acactggcgg
     1981 cctggctggt cgaaagctcg cagaatctga tgatgcctgc ctattacctg atggtagtgg
     2041 cggtgattgg tttaatcacc ggcgtaacca tgaaagagac ggcaaatcgt ccgttgaaag
     2101 gtgcgacacc ggcggcgtca gatatacagg aagcgaagga aattctcgtc gagcattacg
     2161 ataatatcga gcagaaaatc gataatattg accacgagat tgccgatttg caggcgaaac
     2221 gtacccgcct ggtgcagcaa catccgcgaa ttgatgaata agctgaaacg gatggcctga
     2281 tgtgacgctg tcttatcagg ccaattgaac tcttaaggtt cacttaatct ctgacgcgca
     2341 tactctcctc caggttaacg gaggagagtg caatgaaaaa ccgtgtttat gaaagtttaa
     2401 ctaccgtgtt cagcgtgctg gtggtcagca gctttcttta tatctggttt gccacgttct
     2461 gatctttctt cagccgtacc caggcccgcg tgccggaagt ctcttgccgg ttttgcagga
     2521 aaaactgccc gtgatgcaac tgtgtaatgc ggctgacaat acttaacccc agaccaatcc
     2581 cgccataacg gctgtccata cgtacaaacg ctttactcaa ctccccgcat ttactctcat
     2641 caatacctgg tccttcatct tcaactgcca tgaccgctcc gccatcttct tgcagcttaa
     2701 tcataatgtt gctgccttgc gggctgtaac gatgggcgtt ttctaccagg tttcgcaata
     2761 acatccgcag cagggttgca tcaccctgaa cggtgatgtc ggcggcgctc tctggcaata
     2821 gcagggtttg ctgtcgctgg tcgagcatgg tactgagttc gtcatacgag gggagaatga
     2881 catcttccag cagttttaca tgttgataat taccggaaga aaatgactgt ccggcacgcg
     2941 ccagttgcag cagctgggag acgctctcca tcatctgatc aagccgtgcc actaacggtg
     3001 ctacatcaat gtgatgcgtt ttcgccagca gttccagatg caaacgcacc cccgccagtg
     3061 gcgttcgcag ttcgtgcgcg acgtcagcgg taaacaacct ttcgttatcc agcgtgctgg
     3121 tcaggcgact gaccagatcg ttcaacgccg aaaccaccgc ttcgatttcg agggtggcgc
     3181 tgtgaatggc aatgggcgtt aagttgtcgg cggtgcgcgc ttccagctct ttttgcagct
     3241 ccgccagcgg gcgggtgatg cggcgtaccg cctgatagca gataaatagc gtcaggctga
     3301 ccataaagac gccggggaca atcaggctgg cgaccgcctc gcggatctca cgcatgatgt
     3361 ggcgatcgtt gttgcgattg tcgcgtagcg cctgctcaaa cagctgaatc tgctcggtac
     3421 tttcatgcca tagccagaag acgctgatca gctcaaacac caacaaaatg gccccgatgg
     3481 tcaatatcag ccgttggcgc agcgatattg gtcggcgcag aaaacgcatc agattcaatt
     3541 agttttcctc attcgcgacc agcatatagc caaagccgcg cacggtgcgg atacgggctt
     3601 tgcccacttt gtcgcgcaga ttgtggatat gcacttccag ggtgttggtc gagggttcat
     3661 tgtcccagtt atagatgtcg ttgtagagaa tttcccgatg taccggactg cctgctttga
     3721 gcattaaccg tgacagcaga gcatattctt tgggcgtcag aatcaactct tcaccgccca
     3781 tccatacctg acggcgaccc atattcagcg tcagattgcc aacaatcagc tcactttcgc
     3841 cctgattatt atggcgtcgt agcagggcgc ggatacgggc gtgtaactct tccagcgcaa
     3901 aaggcttcac cagatagtca tcggcaccga catccagccc ggcgattttg tcggtcagcg
     3961 tatcgcgagc ggtgaggatc agtaccggca gggtgtattt tttctgccgg atacgggcga
     4021 gaaaatgcag tccatcttcg tcgggtaacc ctaaatccag taccaccagg ctgtaatgac
     4081 cgtcctcaag gctttgttcc gccatccgcg cggttgtcac gccatcgcac gcgtagcctt
     4141 cggtttgcgc cgccagaatc agtccctgca ataacagcgt atcgtcttca acaatcagaa
     4201 ttttcattta ctcactctcc tgcaagtttg cagaatatca tccgcggcct ggtaatactt
     4261 cgtctcaacg ccagttaatc ccaatagcgt ggagaataaa ttgtcttgtg aatagtgttg
     4321 cgtttgcgcc tgtttttgca ggcagttctg gtcaacctga taccgctttt gataatcctc
     4381 cgacagccac agcagcattg gcacctgttt ttggctatcc ggggcgatgg cataaggcag
     4441 accgtgcaga tagatgccat tttcacctaa cgattcaccg tggtcagaaa gataaaccag
     4501 gctggtggta aatttatcct gatgttcttt aagcagatta atcgctttat caacaatata
     4561 gtcgacgtaa accagcgtgt tgtcgtaagt gttcaccagt tgctctttgg tacaggtctg
     4621 gatctcattg gtgtcgcagg ttggggtaaa tttcctgaac tgcggcggat agcggttgta
     4681 ataggtcgga ccgtggctgc cgatggtgtg taagacaatc acgccatcac cctgcaggtt
     4741 attgatgtac tcttccagcc cgtggaacag cacttcgtca tagcattcgc cgttgatgca
     4801 ctgatcaggc agattcagcg cggtgacgtt ctggtgaggc acgcggtcgc aggcaccttt
     4861 acagccgcca tcgttgtcat tccacagcac gttgatgccc gctcgctgaa tgatatccag
     4921 cacgccttcc tggtgctgtg ccagctcttc tttgtagtgc tcacgcggca tatccgagaa
     4981 catgcacggt actgaaactg ccgttgccgt gccgcaagat gcggtattag ggaaatagac
     5041 cacgttatct ttcgccagcc gcgggttagt tgcacgcggg tagccgttga gggagaagtt
     5101 ctccgcccgc gaggtttcgc cgacaatcag gatggtcaaa tttttacgtt tttcgttctg
     5161 cattaacggg ttgcggtgcg cgtcttcacc aattcgcacc agcggcagat ttgccagtcg
     5221 ctgatgggag taccatgacc agctggcaac aatgctgtta gaggggctta aggatttcac
     5281 cagctctttg ttattgcgga acaacgaggc gtagtcttta taaaacagtg cggcgaccag
     5341 caaaatcagc agtacagaaa ccagaatatt ggctccacgg aaaagaacac tgcgcagacg
     5401 cgaggtggca ggtttgattt ttagccagca ggcaatcagc gcagcaagca cgccgctgaa
     5461 tcccagcgtt aataacattt gcggtgtcat cagcgcataa ctttctgctg gagtggtatc
     5521 aataatattg gcaatcatcg agcggtcgat gacgatgccg taagtcatta tgaaatattg
     5581 tgcagccgcg ccaatcagaa taaacaggca ggccagtggt cgattaagcc ataagaaaga
     5641 gcttagtgtc aggacaatat taatcacgct gaaagcgacg accggcatcg acaagaaaac
     5701 cagtacgtta tgcagcgaat ccagcggcag cgcctgcaac acctgtttaa aaaaggcaat
     5761 attcaggcag atagagatat aaaaagcggc caacaatagc caggcgagta aattcaaaga
     5821 gggtcttttt agtaggcgct tcaacatcac ggtgtttcca tcgaacaaag tgcgcatatg
     5881 ctcgcaaagc aaaattaagc caaccttaag ttcttaaggt tggcttttat gtttgctgga
     5941 ttaaggggaa ttaatctttg cttattggtg catctaaggg atacgggttt ttatgtagcc
     6001 ggttgtaatt cagcgcatac atggcggtga tgaccatcag ggtgacaaat gaccacataa
     6061 cctctttcgc cccggacccc accacggccc aaatgcagta gaggaaggca atggtagtaa
     6121 cagccagata tgccgggcgt gctttaccaa agtgaccgtg gccgagcagc agtaacgccg
     6181 cacaggtgta aagatatggc accagtgtaa agatgaccga gacggaagaa accagaccga
     6241 actctttggt cgcgtttggt gaaatgctgc tgagctggaa gatggtcatc aaaataccga
     6301 cgataatcag ccccgccact ggcgtacccg ctttatttac acgggcaaaa atcggtggga
     6361 acagtccgtc atcggcagcg gctttcgccg tttgacccgc cagcaacgtc cagccgccca
     6421 gtgaacctaa gcaacccgca gctgcgcaga aggaaacaat ggccccagcg gtgtcaccca
     6481 acgccatccg tgcggcatca ccgaatggcg aggcagaaac gcgcagtgcg gcattaggga
     6541 tcatccccat aatcgcggtg gtggagagca catagcaaac ggcggcaatc aataccccac
     6601 caatggtggc gatagggaca ttgcgtttcg ggtttttcac cacacctgcg gcaacggagg
     6661 cactttccac accgatgaac gaccacagcg taacgttaag ggtactttga atcgcaccga
     6721 aggtgcccag gccgctgacg ttccatgccg ccatataggt ttcaccacgg aaccagaacc
     6781 agccaaatac ggcaatcccg acgatgggga tcagcgccag cacagtggca acggcctgca
     6841 cgcgggtgat cattttcgga ccgacaatgt tcagcaggac gaagatccac agcaccacga
     6901 cgcaggtgat ggttaatacc aatggatctt tcagaatcgg gaagaagtaa cttaaatatc
     6961 ctacgccaat gaccaccatg gcgatattgc cgatccagca ggccagccag tagaggacgt
     7021 tggtttgata accgagaaac gggccaaagc agcggcgggc gtaagcgtaa gaaccaccag
     7081 gacttgggtc gaggaacgac attttggcgt ataccatcga gagccccagc gcaccgataa
     7141 tcgtcaccaa ccatccataa atggcaatcc cgccagtgga ggccaggttt gcaggtaaca
     7201 gaaaaacacc tgaccccata atattccccg acaccatcag ggtgacgggg attaagccca
     7261 ctttgtgagc atcagcatcc gaagacataa ttaaactcct gcgaaggcga gcttcgtgac
     7321 aataaattac gtcatatcat acgcctgcat tcgatagttg aattattatc gaccgggtta
     7381 tcgctgatgc cgttattaat atagctcttc gttaaggtta aaaatcaggc gacagtgcgt
     7441 tctctgtgct gactaacata atgcagcggc gtcataccgt agaagtcttt aaagacagaa
     7501 ataaagtacg acgtactgtt gtagccgcag gactgtgata cctgagagat atttttaccg
     7561 tccatcatta attcatttac ggcataacgc atgcggcagg tggtgattat ttggctataa
     7621 ctggtgtttt cgcttttcaa ctttttcttt aacagacttg ggctaagaca taaacaactg
     7681 gctaccatac taagattcca gtctttgtga atatcgcttt caataatttg ataaacgctg
     7741 tcacttacac aattacgtaa catatacatc attaatgaaa ggaatttttt actgtcgaga
     7801 aaacgggata gcaccgtgaa taataatgcg cgtgttcgtt ccttttctga ctcagtttct
     7861 gcgggcatca tgctgtgttg cgcggcaacg cgaaagacgt ctggcgtcag gcatggcagg
     7921 gtgagtatgg gcgtagcgct acgttgccat actggtattt gcgagagatc tttattcaga
     7981 aaccggaggt aatctttgat gatgtcgtga ctaatatgag caaccaaagt gttgttaaga
     8041 gaggagacgt cgataatatt attttcacaa tttaataacg ccatatggtt agctttaagg
     8101 ctaataggtt ctttcccatt cacccttatc cagacatctt tttcagtcaa taaaacaata
     8161 caaggttggt cgctgcaaat cctcatacat gtactcctga gtgcgaataa aagaccagtt
     8221 ctcgctaaag caaagcgata ccgggaaatg gttatataat tgcatgatga atataaacat
     8281 acataaaaat aacgagtttt actaatatta caaattttta tagtaatttc tcatgtacat
     8341 atttttttac aggataaata acaggcaaaa aaaagcgcgg ggtggggccg cgcaaaaagt
     8401 atgacatttt tgtttgcagc aatttttatg aaaacgttgc catactcaaa aggcctaagg
     8461 ccaggcggcg taatgttatt taaacaatta cgccttcagc ggaatagtgg ttacgctttc
     8521 acgcacataa cgtggtaaat accgtcaata atttcagtcc cttcagtttc gtgttcaaat
     8581 ccagggaaat ggtggtccca ggattgcagc gagcgtaaat aacttacttg cggactgttt
     8641 ttatcgccga agttttcacc agacagcagc atcgggattc ctggcggata cgggataact
     8701 gagtttgccg cgatgcgtcc tggcagattt tcaatggata ccagttcgac attgttgtcg
     8761 acaatcgcgt tgtacgcttc acgcggggtg atttccgcca ccggcaggcc ggaataggct
     8821 tcgttcaacc gtgcgccagg gttgttttct ttcagccagg caaacatggt gtcacccaga
     8881 tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt caacaagttc cggcatcacc
     8941 tgcgccagcg gtgtgttggc gtcatagtgg tgtttgaagg agcaaagggt gttaaccaga
     9001 gtcccccatt tcccacgggt tacgcccata gagaacagga acataatttg gaagtcagtg
     9061 gtgcgggtag gcacaatgcc gtggcgacca agccaggcag tgaccagcgc cgccggaaca
     9121 ccggtttctt ccagttcacc atcttcaccc attcccggag caaggatgct gactttaatt
     9181 gggtcgagca tactccagtt atccggaata tctttgaagc cgtgccagct ttcgcccgga
     9241 tgcattaccc agcagtcctg aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca
     9301 taggttttgc cggtttgtgg gtcggtgacg acttctttgt tccacggttt gaagaaccag
     9361 ctaccgtcag cggtgaactc tttatatagc cgcgccatcg cctgacggaa atcaaccgct
     9421 tcgtcaatca cttcctgtgt cagtgacagg ccgctgttgc cgtccatcat cgacaccgcc
     9481 acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg tggcatgcat catgtaggcc
     9541 tggttgaagc gggagaagtt aatcgcccca cgaccttcac gtacatgaat ataagaagcc
     9601 tgtgacagcg cattcagcag tttgtggatg gagtgggtgg cgaaaacggt aggaccgttg
     9661 tgatcgccag gttcgccgcg catggcatag tgatcggcat agatcgggtt gaaacgtgca
     9721 tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg ttttttccag cagatcctgc
     9781 gcttctttag cgttataaca cacgccgtca taggtgcagt tggttaccac gcagtaagac
     9841 ggtttttgcc cggctttgtc tttggtcagc gggctttcac tgattttctt ttgcaaggtt
     9901 tcaggttgca tttcctgcgg atagattggc ccgataatgc cgtagcggtt gcggcttggc
     9961 accatataga ccggtttcgc gcctgtcagc atcaaacctt gttcgatgga tttatggcag
    10021 ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta
    10081 gagccggaag taccgacgac taccgaccag gagcgatcgg caccaaatac gcgtgcggca
    10141 tatttttcgc tttcgccaaa tgcgccagta tggtcaagca aagaaccgag ggaagttcgt
    10201 tcgatgccca tgtcggtgcg gaacagattt tcaccatagt agtcatggta gaaacgtccg
    10261 gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg gcgctgccca ggaatattca
    10321 tggatgtcac tatatttcat cagcgcgctg aacagtggcg gcaacagctg ctggcggtag
    10381 cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg cggtatcttc cagaatccag
    10441 gcgaattcat cgacaagctc cagcagatcc cggtccattg cggcgagggc tttttcccga
    10501 tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat gaagcttacc gatcaattgt
    10561 ctgacgtttt gatgttcgtc cggatgctcc atttgatagc tgaacatcag gcagtcaatg
    10621 gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg aggtggattt aatcacggta
    10681 acattttgct ggcttaaagc atctgccaga cgctcaacgg cgttaccgac ccaggtgtct
    10741 tgatggagaa actcgctttc aacaattaat actttcatca ttgcttaccc ggttatgaag
    10801 gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg ctgtaaagcg cgtgaaaaac
    10861 aaacattttt gaaatattta gttttataaa caatacgtta tgtgattatt ttaaattatt
    10921 ttcactgctt attatcgacg gctaaactat ttttttggct gatactgata tcgtctttag
    10981 ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg cgacatgccg
    11041 acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc cgtcagggca
    11101 atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt gatgcgcatc
    11161 gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc cattgcatag
    11221 ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg atcatagttt
    11281 tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga gacgctgttt
    11341 ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agctagagag gctaaatcgc
    11401 ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcatt cggactgttt
    11461 aattcctgct gccagcggcg tacttcaaac gggctaagtt gctgtgtggc aagtgatttg
    11521 atcaccatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca ggagagaaac
    11581 agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc tgttagttgg
    11641 tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac tttttcattg
    11701 ttgatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg accatgccag
    11761 tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg gtattccgaa
    11821 tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa cgtatctgta
    11881 ttcatggatg gctctctttc ctagaatatc agaattatgg caggagtgag ggaggatgac
    11941 tgcgagtggg agcacggttt tcaccctctt cccagagggg cgaggggact gtccgagcat
    12001 catgaggccg aaaactctac ttttcaggta atttattccc ataaactcag atttactgct
    12061 gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag gttttctgca
    12121 gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg atgtctgcac
    12181 ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt cttggtgatg
    12241 tgttccatcg cgaggcgctg aaaacggcgc atattgccct gatggacatt gaccccaccc
    12301 gcctggaaga gtcgcatatt gtggtgcgta agctgatgga ttcagcaggg gccagcggca
    12361 aaatcacctg ccacacccaa cagaaagaag ccttagagga tgccgatttt gtcgtggtgg
    12421 catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc tgtaagcggc
    12481 atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg cgcgcgctac
    12541 gtaccattcc gcatctgtgg caaatttgcg agaacatgac ggaagtctgc cccgatgcca
    12601 ccatgctcaa ctatgttaac ccaatggcga tgaatacctg ggcgatgtat gcccgctatc
    12661 cgtatatcaa acaggtcggg ctgtgccatt cggtgcaggg aacggcggaa gagctggcgc
    12721 gtgacctcaa tatcgacccg gctacgctgc gttaccgttg cgcaggtatc aaccatatgg
    12781 cgttttacct ggagctggag cgcaaaaccg ccgacggcag ttacgtgaat ctctacccgg
    12841 aactgctggc ggcttatgaa gcagggcagg caccgaagcc aaatattcat ggtaatactc
    12901 gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc gtcacggaat
    12961 cgtcagaaca ttttgccgaa tatacgccgt ggtttattaa gctaggtcgt gaggatttga
    13021 ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag cagctggcga
    13081 actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt aaaccgtcac
    13141 gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagccgagt gtgatttacg
    13201 gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc gtggaagtag
    13261 cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta ccttcgcatc
    13321 tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa gctattctta
    13381 cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact gccgccgtgc
    13441 tgggcattga cgaaatatat gctcttgttg acgacctgat tgccgcccac ggcgactggc
    13501 tgccaagcta gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg ggatttattc
    13561 cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca gcgacccgat
    13621 accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg ttcgggaagg
    13681 attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat gtcgtcgggc
    13741 tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat gctattaacg
    13801 atccgattat gggatggatt gtaaatgcta cgcgatcgcg atggggtaag ttcaaaccct
    13861 ggatcctgat cggtacgttg gcaaactctg taatcttatt tctcctcttt agtgcgcatc
    13921 tgtttgaagg tactactcag attgtctttg tttgcgtgac ctacatcctc tggggcatga
    13981 cttacaccat tatggatatt cccttctggt cgctggttcc aaccatcacg ctcgataaac
    14041 gtgaacgcga acaactggtt ccttatccgc gtttttttgc cagtctggca ggctttgtta
    14101 cggcaggtgt gacgctacca tttgttaatt atgtcggcgg tggcgatcgg ggatttggct
    14161 ttcagatgtt cactctggta ttgatcgcct tttttattgt ttcaaccatc atcactctgc
    14221 gcaatgtgca tgaagtcttt tcgtcagaca atcaatcgtc tgctgaagga agccatctga
    14281 cacttaaagc catcgttgcg cttatttata aaaacgatca gctttcatgc ctcttgggta
    14341 tggctcttgc ttataatgta gccagcaaca ttattaccgg atttgctatc tattatttct
    14401 catatgttat cggtgatgcg gatttgttcc cctattatct gtcgtatgcg ggagctgcta
    14461 acctggtgac gttagtattc ttcccacgct tagttaaatc attatcccga cgcattttat
    14521 gggccggagc atctattctt ccggtgttaa gctgtggtgt tctcctgtta atggcattaa
    14581 tgagctatca caacgtcgtc ctcattgtga ttgcgggtat tttgctgaat gtgggaacgg
    14641 cgcttttctg ggtattacag gtcatcatgg tggcagatac cgttgattac ggtgaatata
    14701 aactgcacgt acgctgtgaa agcatcgctt actccgtgca gactatggtg gtgaagggcg
    14761 gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg gatgattggc tatgtaccga
    14821 atgttgaaca gtctacgcaa gccctattag gtatgcagtt tattatgatt gctctaccga
    14881 ctctgttttt catggtaacg ctgattctct acttccgttt ctatcgcctc aatggcgaca
    14941 cgctgcgcag gatccagatt catctgctgg ataaatatcg caaagtaccg cccgagcctg
    15001 ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa agcctgacgt ggacaatagc
    15061 taacgaaaat ggcctgatgc gatatgttta tcaggccacg tcttaatgta atgctttgga
    15121 tttgcaaatt ttgtagaccg gataaggaat tcacgccgca tccggcatca acaaagtgca
    15181 cgttgttatc cagttatcaa gccatagcgc cgtagctggc ggcaatgaaa gcgagcgagc
    15241 aatatcactg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg cgtgtcccac
    15301 tggtcgccct gggtaccgag aaaatcatcc gctccctggc ccatcgccag tgccgcccac
    15361 cactcgatta attcatacat cgcgcttatc gccagcgcca cgcaacagac cagaaacgcc
    15421 accattttac gtccgcgcac gtacatcccg cgaacgagaa tttctcgtgc caccagtgca
    15481 ggcaccagcc cctggaaaaa gtgccccagc ttgtcatacg gattacgact caaccccagc
    15541 cattcctgca cctcaaaacc aacggggact ttcgcgtagg tgtactgccc gcctaccatc
    15601 aggatgatgg cgtgaaagaa gatgagcgta tagagcagca gcgttaacgg gtaacgtctg
    15661 gcggtggcaa gtagtagcgg cacgacgata atcaccggtg tcacttccag cagccaggtg
    15721 agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc cagtgcgccg
    15781 gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga gttcgcaagt cagggaaaat
    15841 actgactatt caccagcaag catgaaatta caatcgcaaa agaggtggcc cagggggatc
    15901 acctggcagc atgctgtcag gcgctgggcc gaagaggtta cttagtgcag ttcgcgcact
    15961 gtttgttgac gatttgctgg aagaagtcgt tacctttgtc atcgaccagg ataaacgccg
    16021 ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga taagcgacgc
    16081 attccagatg cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg atgctaccga
    16141 ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg ctggctgcgg ttgcctttcg
    16201 ccagcatgat catgctgccg ccgtgggatt gcagcagatc cacataggag tccatacgac
    16261 ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggggttttc gccggacccg
    16321 cgtagtagat cgggtgatct ttgatgtact gcggaagttc tttaccggcg tcaatcagct
    16381 ctttcagctt ggcgtgtgca atatctcgac ccacaataat ggtgccagtg agcgacaaac
    16441 gagtggaaac cggatattgc gaaagctggg cgaggatctc tttcatcggg cggttaaggt
    16501 cgaccttcac cgcttcgcct tcaccggcct gacgcagttc ttgtggaatg tactgaccta
    16561 gattgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct ttaatgttac
    16621 ggtcagcgga gcaggagacg cccatgccta ccgggcagga tgcgccgtga cgtggcagac
    16681 ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca agaccgagtt
    16741 tctgggcctc ttccagcagt tcctgttcca gctggacatc gcggaacgcc tgaccatgtt
    16801 cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaatttg acggttttca
    16861 ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc gggcaggctg
    16921 cagtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg ggagtcagca
    16981 gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct ttcgcaacgc
    17041 aaaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca ggcaggttag
    17101 tgccggtgtt gacctctttg tacatgtcca gcgccgcgtt ttgcgagtag cgcaggttat
    17161 cttcgatata ggtgttatag acgcctttcg acagcgcttc ttcatcaccg ccaccggtcc
    17221 acacgcgctg gcctttttta ccgacgatga tcgcggtgcc ggtatcctgg caggtcggca
    17281 gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagtgccacg tacttgtcgt
    17341 tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt gccggacgta
    17401 gcatgaaaga agcgtcgtga aaggcttgct gcgccagcag ggtcagtgct tctggttcca
    17461 ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa gtgagtagat
    17521 agtattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag ggtttgtttg
    17581 acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc cgcgtagtca
    17641 cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga tccagccgaa
    17701 gacgcacgat acgctcacac caatcaaccc cggcagaata aaactgtggt tgatgacgaa
    17761 gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat cgctcggata
    17821 agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt atgccggatc
    17881 aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct gagagtttac
    17941 aaacttggaa accagcagca gaacaatggc atacgcccac ggatactctt tcaccatttc
    18001 acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttctg ccatccatgc
    18061 gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt tttttgagat
    18121 agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca gcataaacat
    18181 ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca ggtccgaatc
    18241 agcaccaaga agggcgacta cagcgattgc cccgaggaaa atccacattg ccagccagtt
    18301 gcttttcggc agttttttat ccagcagcgt cgcggtatca ccgtaaacat actcacggtt
    18361 ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt taccgcggaa
    18421 ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg tgattgccag
    18481 cagatcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca tcgcaaccag
    18541 agacacgacc gcaaccgaca ccggactggc gataatcccc atctgtgcac cgatagaact
    18601 tgccgccatc ggacgttccg gacggatgtt gttcttaatg gcgacgtcgt agatgatcgg
    18661 cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagtgtac aggtcacaaa
    18721 cggcgcgaca attgagacat atttcgggtt gcggcgcagc agcttctcgg caatttgcag
    18781 catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca ccgcaatgat
    18841 aaccagcatg acatcaactg gtggtttacc tggctgaagg tggaagacga agaccagaat
    18901 gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg caccataaaa
    18961 cagacatatt agtattatga taagttggat agtaaataac atgtgtgaac cctcgcgata
    19021 atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta tgaactgttt
    19081 tcagagcctg actaaatatc cgtttggtcg ttaccggctt tagcaaatac ctcacagtga
    19141 atattggctg attaaagcag cggcaaattt atgcactgtg ataagcggct ttttcagaga
    19201 gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc agatgttaat gttttgtttg
    19261 ctgttatctt attgatattt atcggtctat tttttgttct cagggcagat atcattaggt
    19321 ttatagatta atctgatcta cccatttgtg ggtaaaaata cacataatgc gggtgacata
    19381 atagttaatt aacttttgtt atcgttttga aattaaaaac accgttcatc tgaagagata
    19441 ttaattttta acgatgatgg agggataatt atatttgatc tggcacaagt tttactgatt
    19501 aacgatgtaa cttgtgccag gggtattttg cattacggta ataattatta cttacagata
    19561 accagcgatc cgttattggc aatattgttt cagcagtgag tagtgttctg cctgaatacg
    19621 gtaacggtaa accggacgcc ccgtgacgcc ataatggata ctggtgaaca agatgtggca
    19681 gttgaccagc cagatgaggt attttcggca ggaaacacgc gagatgttaa cctcgttagc
    19741 cagctcgtcg gttgaaaact catagtcctg atgcgcgtca atccactggc acagcgtgcg
    19801 taacgtctgc ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt tggagctgct
    19861 gccgtggatt agctgatcaa gctcggcctg gtcataatac tgatgttttt ccagcgccat
    19921 tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga agggtttgat
    19981 caggtaatcc acgacaccgt aatgcagcga atctttaatg gttgccgcat cggctgcgga
    20041 ggagatgaca atcacatcac ttttgcaacg cgcgttatgc aggacaggca gtaaatcgag
    20101 cccgttctct ttttgcatat agatatcgag caatatcagg tcgataggcg catcgctatt
    20161 gaagataatc tctttggctt tctccagcgt cgaggctgtt ccacagcatt gaaagcctgg
    20221 gatttgtgct acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat cgataattaa
    20281 tacattgatc atctgttcga cctctccccg tcccagggta tctggacaaa aaattgtgtg
    20341 aaaatccccg gttccgattc cacggcgatg ctgccgccga gattttctac ctgttgtttg
    20401 acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt gtcaaaaatg
    20461 tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt taacttcgca gtgcagccag
    20521 ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg gttccggccc taatgcttcc
    20581 agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg gtcctcactg
    20641 ccgctgtctg gcagctggct ttcactgttt aaaatcagcg tatggcctaa atcggtcgcg
    20701 cggttaatct tgctgattaa aaaaccagcg ataaccggag atttgatctt acccagcaga
    20761 gagccaatct cttcctgata gttattggct gttttgagaa tgtaatcttc caactgctta
    20821 taactcttca gatgcaataa tccgagaatc acatgcaatt tattcataaa ttcgtgggat
    20881 cgttcacgaa gtgcgtcagc atagttgacc agaccgtcga gtcgctgcat cagtttacga
    20941 acttcagttt tgtccctgaa ggttgaaatg gcaccgatga taacgccatt actgcgcacc
    21001 ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc gcggcgcggc
    21061 gtaccgtcgc gtaacacttc cgagacatct accacctgtg accatgagtg gctgagcgtc
    21121 gacagtttct catcgtcctg cgacttacgg taattcagca attcttgtgc ggcatcgttg
    21181 atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc cttctttgat agactgcaac
    21241 atggcctggc gttgctcaaa cagcgtggag atttcgtagg gttccaggcc gaaaaggatt
    21301 tttttcagta ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat gccaaataat
    21361 accgaccaga taatgctcca gcgactgtca ttgatctgtt gggtcacgcg gcttaactca
    21421 aggccgatcg ccaccacgcc aatttgttta tgattttcat cgtagatggg ggtaaatacg
    21481 cgtaaagcct gcgccagaaa accgcgattg atagcgacat tttcttcgcc attcagcgca
    21541 ttaaggatgt catcaccttt aaatggctga ccaatacgct gggcttcagg atgtgagtag
    21601 cgaagacttt gcatatcggt aacgacaata aacagcagat cgttgcgttt gcgcacggct
    21661 tccgcgatgg cctggatgcc actctcctgc ggttttttct gcaagccctg acggatttcc
    21721 ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt tggccagccc gtctcgcgtc
    21781 atatcactga tttgcgagaa gtaaatcaga tgcaccacca atagcaccga gaacagtacc
    21841 gcgctgacca ttaagatcac agtggtactc aatttcatcg gacgtttgcg taatatgtgg
    21901 tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc attatctctg
    21961 atgaggcggg taattcaaag ggagtaaaaa tgattggcta tataggggaa gagactctgg
    22021 caacggaaac tgccagtgct gtatgaagat tccggggcta tgcttatagc gataatcata
    22081 ctgatgagag agggaaggtc atggatcagg cgctactgga cgggggttat cgctgttata
    22141 ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg tcagcattct ggcaattgcg
    22201 tacgtggcaa cggcaagtta tttaatctca aacgaaagtc gt
//
LOCUS       DADLCQ010000061        22106 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000061 DADLCQ010000000
VERSION     DADLCQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.061"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..408)
                     /gene="sxy"
                     /locus_tag="JQG95_002211"
     CDS             complement(<1..408)
                     /gene="sxy"
                     /locus_tag="JQG95_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY5888753.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
     gene            627..1136
                     /gene="sulA"
                     /locus_tag="JQG95_002212"
     CDS             627..1136
                     /gene="sulA"
                     /locus_tag="JQG95_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY5888754.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            1492..2538
                     /gene="ompA"
                     /locus_tag="JQG95_002213"
     CDS             1492..2538
                     /gene="ompA"
                     /locus_tag="JQG95_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY5888755.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(2614..3066)
                     /gene="matP"
                     /locus_tag="JQG95_002214"
     CDS             complement(2614..3066)
                     /gene="matP"
                     /locus_tag="JQG95_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY5888756.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            3251..5011
                     /locus_tag="JQG95_002215"
     CDS             3251..5011
                     /locus_tag="JQG95_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY5888757.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            5080..5598
                     /gene="fabA"
                     /locus_tag="JQG95_002216"
     CDS             5080..5598
                     /gene="fabA"
                     /locus_tag="JQG95_002216"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY5888758.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="JQG95_002217"
     CDS             complement(5668..5835)
                     /gene="rmf"
                     /locus_tag="JQG95_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY5888759.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="JQG95_002218"
     CDS             complement(6091..6654)
                     /gene="pqiC"
                     /locus_tag="JQG95_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY5888760.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="JQG95_002219"
     CDS             complement(6651..8291)
                     /gene="pqiB"
                     /locus_tag="JQG95_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY5888761.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="JQG95_002220"
     CDS             complement(8296..9549)
                     /gene="pqiA"
                     /locus_tag="JQG95_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY5888762.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(9679..11586)
                     /locus_tag="JQG95_002221"
     CDS             complement(9679..11586)
                     /locus_tag="JQG95_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5888763.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="JQG95_002222"
     CDS             complement(11598..13706)
                     /gene="rlmKL"
                     /locus_tag="JQG95_002222"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY5888764.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            13950..15059
                     /gene="ycbX"
                     /locus_tag="JQG95_002223"
     CDS             13950..15059
                     /gene="ycbX"
                     /locus_tag="JQG95_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY5888765.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRTNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="JQG95_002224"
     CDS             complement(15056..15598)
                     /gene="zapC"
                     /locus_tag="JQG95_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY5888766.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="JQG95_002225"
     CDS             complement(15772..16782)
                     /gene="pyrD"
                     /locus_tag="JQG95_002225"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY5888767.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(16893..17632)
                     /locus_tag="JQG95_002226"
                     /pseudo
     CDS             complement(16893..17632)
                     /locus_tag="JQG95_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(17598..18113)
                     /locus_tag="JQG95_002227"
     CDS             complement(17598..18113)
                     /locus_tag="JQG95_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5888768.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(18121..18663)
                     /locus_tag="JQG95_002228"
     CDS             complement(18121..18663)
                     /locus_tag="JQG95_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5888769.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGYFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(18761..19744)
                     /locus_tag="JQG95_002229"
     CDS             complement(18761..19744)
                     /locus_tag="JQG95_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5888770.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGLRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIRE"
     gene            complement(19735..>22106)
                     /locus_tag="JQG95_002230"
     CDS             complement(19735..>22106)
                     /locus_tag="JQG95_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY5888771.1"
                     /translation="VLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPA
                     FKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINA
                     LFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSYL
                     QRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKSN
                     AQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSVP
                     LLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAAM
                     FGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRYS
                     TSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYGL
                     FYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFSL
                     PLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQGY
                     NSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQSL
                     GETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDLE
                     NAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDD
                     GSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         5479 a         5794 c         5194 g         5639 t
ORIGIN      
        1 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
       61 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
      121 caccagcttc agttgatttc gccatagact ttcatcaacc cgatagtaat tgagggtaac
      181 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
      241 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
      301 gtcaacggtc aggctgtaac tgccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
      361 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
      421 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
      481 acttattctt aaggggcaag attaatttat gttttcccgt caccaacgac aaaatttgcg
      541 aggctctttc cgaaaatagg gttgatcttt gttgtcactg gatgtactgc acatccatac
      601 agtaactcac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
      661 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
      721 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
      781 cattgttaca gcaactcggt cagcaatcgc gctggcaact ctggttaaca ccgcaacaaa
      841 aactgagtcg ggaatgggtt caggcatctg ggctaccctt aacgaaagta atgcagatta
      901 gccagctctc cccttgccac acagtggagt caatggttcg cgctttacgc acgggcaatt
      961 acagtgtggt gatcggttgg ttgacagatg atttgactga agaagagcat gctgaacttg
     1021 ttgatgcggc aaatgaaggt aacgctatgg ggtttattat gcgtccggta agcgcatcct
     1081 ctcacacaac gagacaactt tccgggctaa aaattcactc taatttgtat cattaagtaa
     1141 atttaggatt aatcctagaa ctttttttgt cgcccagcca atgctttcag tcgtgactaa
     1201 ttttccttgc gaaggcttgt ctgaagcggt ttccgcgttt tttttctgta aattgtcgct
     1261 gacaaaaaga ttaaacatac cttatacaag actttttttt catatgcctg acggagttca
     1321 cacttgtaag ttttcaacta cgttgtagac tttacatcgc caggggtgct cggcataagc
     1381 cgaagatatc ggtagagtta atattgagca gatcccccgg tgaaggattt aaccgtgtta
     1441 tctcgttgga gatattcatg gcgtattttg gatgataacg aggcgcaaaa aatgaaaaag
     1501 acagctatcg cgattgcagt ggcactggct ggtttcgcta ccgtagcgca ggccgctccg
     1561 aaagataaca cctggtacac tggtgctaaa ctgggctggt cccagtacca tgacactggt
     1621 tttattccta acaatggtcc gacccacgaa aaccaactgg gtgcaggtgc ttttggtggt
     1681 taccaggtta acccgtatgt tggttttgaa atgggttacg actggttagg tcgtatgccg
     1741 tacaaaggcg acaacatcaa cggcgcatac aaagctcagg gcgttcagct gaccgctaaa
     1801 ctgggttacc caatcactga cgatctggac atctacactc gtctgggtgg tatggtatgg
     1861 cgtgcagaca ccaaggctaa cgtacctggt ggcgcatcct ttaaagacca cgacactggc
     1921 gtttctccgg ttttcgcagg tggtgttgag tacgcgatca ctcctgaaat cgctacccgt
     1981 ctggaatacc agtggaccaa caacatcggt gacgcaaaca ccatcggtac tcgtccggac
     2041 aacggtctgc tgagcctggg tgtttcctac cgtttcggtc agggcgaagc agctccggta
     2101 gttgctccag ctccggcacc ggaagtacag accaagcact tcactctgaa gtctgacgtt
     2161 ctgttcaact tcaacaaagc aaccctgaaa ccggaaggtc aggctgctct ggatcagctg
     2221 tacagccagc tgagcaacct agatccgaaa gacggttccg tagttgttct gggttacact
     2281 gaccgcatcg gttctgacgc ttacaaccag ggtctgtccg agcgccgtgc tcagtctgtt
     2341 gttgattacc tgatctccaa aggtatcccg gcagacaaga tctccgcacg tggtatgggc
     2401 gaatccaacc cggttactgg caacacctgt gacaacgtga aacagcgtgc tgcactgatc
     2461 gactgcttgg ctccggatcg tcgcgtagag atcgaagtta aaggtatcaa agacgttgta
     2521 actcagccgc aggcttaagt tatcgtctgg tagaaaaacc ccgctgctgc ggggtttttt
     2581 ttgcctttag taaattgaac tgactttcgt cagttattcc ttacccagca atgcctgcag
     2641 atcctgctta agagaagaca ttttattcgc gtatttctct ttgttttccg catcttcaat
     2701 cagctgaaca atcgtttcag aaagcgtttt accgcgacgc tgtgcaagac cagccagacg
     2761 ttgccagaca ataaattcca gatcgatcga ttttttgcgc gtatgctggt gttccgcatt
     2821 aaaatggcgc ttccgtctgg cccgaatggt ctgcttcatg cgattgacca gttccggatt
     2881 catatgcttg tcaatccagc catttaccag cacgggttca ttttcgagcg agagcaacac
     2941 atcgacggct tcctgggcgg cactggcttc tatgtaacgg gtaattaact ccccttcgcg
     3001 atgcttcttc accagatact tccatttcca accgctttca agattttcaa gttgttgata
     3061 tttcatagcg atctcaatgt taccgtgtaa ctctttacag aatatcagct ttttaggcca
     3121 gtgaagaaaa gaatctccat cctgtgagct ttaacgccca gccaggagta ttgtcggtgc
     3181 tttctacgtg tgctgtagtg ccggttacgg tataatcgcg gctttgacaa cagactaaaa
     3241 aacatcaact ttgaccatta cgaaacttgc atggcgtgac ctggttcctg ataccgatag
     3301 ctatcaggaa atatttgctc agccacattt gattgacgaa aacgatcctt tattcagtga
     3361 tactcaaccg cgactgcaat ttgcgctgga gcagttgctg catacgcgag catcctcctc
     3421 ttttatgctg gctaaggccc cggaagagtc tgagtatctg aatcttattg ccgatgccgc
     3481 gcgtacgcta caaagcgatg caggccaact ggtgggcgat cactatgagg tttccggcca
     3541 ctccatccgc ttacgtcacg cagtgagtgc agatgataat tttgcgactt taacgcaagt
     3601 tgtcgctgcc gactgggtag aagcggagca actctttggc tgcctgcgcc agtttaatgg
     3661 cgacattacc ctgcagcctg gtctggtgca tcaggcaaat ggcggtattc tcatcatctc
     3721 tttgcgtaca ctgctggcgc aacctctgct gtggatgcgg ctgaaaaata tcgttaaccg
     3781 cgagcgtttt gactgggttg cgtttgatga gtcgcgccct ctccccgtct ctgtgccttc
     3841 gatgccattg aagctgaaag tcattctggt aggcgaacgc gaatcattgg ctgatttcca
     3901 ggagatggag ccagagcttt cagagcaggc tatttatagc gaatttgaag atactctgca
     3961 gattgtcgat gcggagtcag taagccagtg gtgtcgctgg gtgacattta ccgccagaca
     4021 taatcactta cctgccccgg gagcggatgc ttggccggta cttatccgcg aagcagcacg
     4081 ctacaccggt gaacaagaaa cacttccgct tagcccgcag tggatcctcc gccagtgtaa
     4141 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
     4201 gcagcagcgt gaatggcgcg aaggtttcct cgctgaacgt atgcaggatg agatccttca
     4261 ggagcaaatc ctgattgaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
     4321 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
     4381 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
     4441 ccatgcgaaa gggatgatga tcatgcaggc gttcctgatg tcggaactac agcttgagca
     4501 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
     4561 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgctgatg tgccagtgaa
     4621 tcagagtatc gctatcacag gttcagtcga tcagttcggc cgcgtccagc cggtcggtgg
     4681 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
     4741 acaaggtgtc attatcccca ctgctaacgt tcgccattta agtcttcaca gtgaactggt
     4801 gaaagcggta gaagaaggca aattcaccat ctgggcagta gacgatgtga ctgacgcact
     4861 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
     4921 ccaggaacgt atcgcgcaag catcgcaaca ggaaggacgt caccgttttc catggccatt
     4981 acgttggctg aactggttta ttccgaactg atcggacttg ttcagcgtac acgtgttagc
     5041 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     5101 tatacaaaag aagaccttct tgcctctggt cacggtgaac tgtttggcgc taaaggcccg
     5161 caattgccag caccgaacat gctgatgata gaccgtgtgg tcaaaatgac cgaaacgggt
     5221 ggtaacttcg acaaagggta tgttgaagca gaactggata tcaatccgga tctgtggttc
     5281 ttcggatgcc acttcattgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     5341 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg ccgcgcgctg
     5401 ggcgttggcg aagtgaaatt cactggtcag gtactgccga cagcgaaaaa agtgacctac
     5461 cgtattcact ttaaacgcat tgttaatcgt cgtctgatta tgggtctggc agatggcgaa
     5521 gtgctggttg attgtcgtct gatctatacc gccagcgacc tgaaagtcgg tctgttccag
     5581 gatacgtctg ccttctgatt atcatcagac agagtatcaa aaggcgaaac ctccgcaatg
     5641 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     5701 tcgccagcct cccagccatt gtgacctttg actcagcgtc tgatagggac acatttcttt
     5761 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     5821 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     5881 attactaaat gtgcaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     5941 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     6001 tgaagaggaa caaatttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     6061 ggagacggtt atatttacaa atctttataa ttaaggtaga cgctttatct cttgtgcaat
     6121 agaagcggct tcctgactcc agacaccggc cagcacttta accatctcat cgtaaccatc
     6181 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     6241 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgttacggt
     6301 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     6361 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     6421 caagttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     6481 cgccagatag tcaggaacag tgacctgctc tacccataac agacgattgc cctggctggc
     6541 ggtactttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     6601 ggagctgcat ccggccagcc acagtgctgc aatcgtcact agccactttt tcattgtttc
     6661 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     6721 tcattgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag gcgctgcata
     6781 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat gctgcgattc
     6841 aattcacgca acgttgactg catatccgtc ggcaactgct gcatcgactg gctggcgagg
     6901 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     6961 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     7021 gcttccatca gtcgttgctg gatttgcgcc aagccgccgc taacagtcgg gataatctga
     7081 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     7141 acatacagtg caccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     7201 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     7261 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     7321 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     7381 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     7441 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     7501 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctaac accgccacta
     7561 agcagcgttg tcaatgagcc catctccaca cgcatccccg ctgacgtcag atcaaccgca
     7621 atgccactat ctttccagaa gcgaacattg ctggtcacca gtcggtcata aggtgcattg
     7681 atgaacagtt gataactgat attgcgtttt tgcgtatcga aggtgctggt ttcaaccgaa
     7741 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     7801 ctatcgagaa tcacacggat acctttcgca tcaggcgggg ccaacggtgg tgagtccagc
     7861 aaatcgtatt tatccatttt gctgcctttc gcgcctggct gcagttcgat ataaactcca
     7921 gacagcagcg ttcccaggcc gctaatccct tcgcgaccaa tctgcggttt caccacccaa
     7981 aagacggtgt ctttatgcag caatttttcc ataccggaat tcagccgcgc tttgatttca
     8041 acgtgcgtca aatcatcagc cagtgtggcg ctttcaacca cgccgacgtc aacgctacgg
     8101 cttttaatgg tggttttgcc accttcaatt ccttccgcat tcgcggtgat cagggtcact
     8161 tccggtccct gatggctgta atgataaaaa agaacccagg ccccaatgag cgccgtgacg
     8221 ataggaaata tccacacggg agaccagttc ttcactttct ggattttggc ttccccatta
     8281 ttagattcca tactgtcagg actcctcatg ctctgattca ggttgacgat cccacgacaa
     8341 acgcgggtca aacgtcatag cagaaaacat tgtcattatg acgactaaag caaacattaa
     8401 tgcacccatt gccggataaa tactcattaa acctcccata cgcaccagcg ccgagagcac
     8461 cgcgataacg aaaacgtcaa tcatcgacca gcggcctaca aactcaacaa cttcataaat
     8521 caaatgcatt ctttcactgt cgcgcttgcc atgccctttg gcatcccaac acagccacgc
     8581 gatggcgatc atctttaacg ttggcaccat aatactggcc agaaagatca ccgcagcgac
     8641 gggataagag ccttcgctcc ataacagaat gaccccagcg agaatcgtcg acggcatctt
     8701 cgagcctaat aaatccgtca ccatgatggg caaaatatta gccggaagat acagcatgat
     8761 ggacgttaca agcagcgcga gtgtccactg caggctgttt ctacgccgaa cgtacccttt
     8821 ggtactacaa cgcgggcaca cgggttcatc agcaggaagg attgccgtac aacaggagca
     8881 cgaacgcagc ccctgacgta tccccgtgac gcctggtttt agcggctggc gcagttctgg
     8941 catcggggcg atgtcgtccc ataaccagcg acgatcaacg cactgaaaag cgcgcagttg
     9001 caggacacaa aataaacacc aggggagaaa gctgctgcct accccaatgc tgccgtaagc
     9061 catcagttta acgaaactga ccagcacacc cgcgaggaaa atctccgcca ttccccaggt
     9121 tttgagttga aaaagcaccc gtgccagttg ctcttttaaa cggaccggta attccgcgcg
     9181 attcaccagt aacagaatgg ttatcagaca aaacgcggga accagttgca caaacaacag
     9241 gaaaaaggtg ccgaggctgg cgtagtcctc agaaaaaagc acgccgggaa tttccagtaa
     9301 tgtaatttca ctggtaactc ctgcaacgtt catattcaca aaaggaaaca agttggacag
     9361 caacagcatg aacagtgcag ccaacgcata ggcggtggga cgctgccgag gggcatccca
     9421 cgccacggtt aacgttgtgc cacaccgggg acatgccgct ttctgaccat gctcaaggcg
     9481 cggtaacgcc accagcatgt cacactgcga gcacaggatg tgcttcgcgg catgatgatg
     9541 ttcgcacatt ggtactcctt aatgatgcgc tattttacag atgactaaca aagtgttggt
     9601 tttcatgcct gataaaccca gttcatcagg caattttacc gttgtcagct gtctctgttt
     9661 aaatcgacta ttttgcgatc agccaccatt ttttaacgct tcaagatact cccagcgttc
     9721 aaaggcttgc tccagctcct gctctgcagc agccatatca gcaagcactt tttgcgtctg
     9781 ctcatgcggc tgactgaaga aggaagcatc cgccacttgc gtctgtaggg cttccagctt
     9841 cgcctccaga tcttcgagca attgcggtag ctgctccagt tcgcgctgca atttatagct
     9901 tagtttgcta ctgctgcgtt ttacagtttc tgcttttggc gcggcggctt cttcgctttt
     9961 tttcaccgca ggctgtttga gtgccacata ttgttcttgc tgaccacggg catcatgata
    10021 accgccaaca taacgaccaa ttttaccgcc gccttcgaag atccagcatt ctgtaacggt
    10081 gttatcgaca aactgacgat cgtggctaac cagcaatacc gtgccctgat agctgtcgat
    10141 cagttcttcc agcagttcca gcgtttcgac atcaagatcg ttggtcggtt cgtcaagaat
    10201 caataagttg cttggtttga ggaacaaacg cgccagcagc aagcggttcc gctcaccgcc
    10261 agaaaacgca cgtactggcg tcatcgcccg tttcggatgg aacagaaagt cctgcaaata
    10321 gcccaatacg tggcgtggct tgccattaac catcacctct tgcttacctt cggcaaggtt
    10381 atccatcacc gttttatcgg gatccagttc cgcgcggtgc tgatcgaaat aagccacttc
    10441 cagtttggtg ccaacgtgaa tacgcccgct gtccgcttga agctgaccga gcatcagttt
    10501 cagcagcgtg gttttgccgc acccattcgg accaatcagg gcaattttgt cgccacgtag
    10561 aacctgggca gaaaaatctt tcaccagttg cttaccgtta acctggtagc aaacgtcttc
    10621 catttcgaaa acgatcttac cggagcggct ggcctcttcc acctgcatct ttgcggtacc
    10681 catcacttcg cgacgttcac cacgttcgcg acgcatcgct ttcagggcgc gtacgcggcc
    10741 ttcattacgg gtacggcgtg ctttgatccc ctggcgaatc cacacttctt cctgcgccag
    10801 tttgagatcg aactcggcat tttgtagttc ttccacgcgc agggcttctt ctttttcctg
    10861 caggtactgg tcgtaattcc ctggataggt caccagcttg ccgcgatcga gatcaacaat
    10921 gcgcgtcgcc atattgcgga taaacgaacg gtcgtgggag atgaaaataa tcgtcccatt
    10981 gaaggtttta aggaacccct ccagccagtc gatggtttcg atatccagat ggttagtcgg
    11041 ttcgtccagc agcaatacgc gcggattgct caccagcgcg cgtcctaatg ccgctttacg
    11101 caaccagccg ccggaaagcg acgacagcgc aacgttagga tctaacccca gttgcgccag
    11161 cacttcgttg atgcggtttt ccagctgcca caggttgtgg tgatccagct gttcctgaac
    11221 cttcgccagt tcgttgagat ttttctcgct cgggtcgttc atcaccaggc gcgaaatatc
    11281 gtgatagcgt ttcagatatt ccgcttgttc ttcaatgcct tcggcaacga aatcataaac
    11341 gctaccctca acgttacgcg gtggatcctg ttgcagacga gctacaatca aatcttgctc
    11401 ataaataatg cgaccgtcat ccagcccttg ttcacggttg aggattttca ttaacgtcga
    11461 ttttcctgcg ccgttgcggc ccaccagaca aacacgttcg ttatcttcga tatgcagttc
    11521 tgcgttatcg agaagcggcg cgtcgctgaa cgacagccat gcgccatgca tactgattaa
    11581 tgacattact attcctttca ggctgcggta atcagccagc agttgtggat ctggcggtta
    11641 cgggcgaaat cctgggagag cgttttttgc gtaatttctt gtgctttcag tcccagtttt
    11701 gccaggccgt cgagatccat acggaagcca cgtttgttgt tcgagaacat gatcgtccca
    11761 cctgcacgca gcagacgttt caaatctttc atcagcgcca gatgatcgcg ctgaacatca
    11821 aacgcatctt ccattcgttt tgagttagag aaggttggcg gatcgataaa gatcagatcg
    11881 aactgttcat ttgcctcacg cagccacgcc aggcaatcgg cctgaatcag gcgatgcgca
    11941 cgcctagtca ggccattcag acgcaggttg cgttctgccc attccagata agtacgcgac
    12001 atatccacgg tggtggtgct gcgtgcaccg cctaatcccg catgcacggt ggcgctgccg
    12061 gtataagaaa acaggttgag gaaatctttg cctttgctca tctgaccgag catacggcgg
    12121 gcgatacggt gatcgaggaa cagaccagta tcgagataat ccgtcaggtt cacccacaag
    12181 tgagcgttat attcggtaac ttcaagaaac tcgcccttct cgcccagttt ctggtactgg
    12241 tttttgccct tatggcgttc acgggttttc agcaccagtt tgtttggcgc aatccccagt
    12301 accgaaatgg ttgcagcgat aatatcgaac agacgctgac gcgctttgtg cgcatcaata
    12361 gtttttggcg gcgcatactc ctgcaccacc acccagtcgg cataacggtc aacggcaacg
    12421 ttatattccg gcagatcggc gtcatacagg cggtaacatt caatcccttc ctggcgtgcc
    12481 cacttctcga attttttgag gttcttacgc aggcggttgg cgtaatcttc cgctaccatt
    12541 gccggtttac tgtctggtgt gctttcggca acgtggtaat ttttctgtac gcagtccagc
    12601 gggccgtttt tcgccttgta ctgtttgtct gcacgcagct gcaggcagct tagcagatcc
    12661 ggcgaggcac taaacaaaga gagattccag ccaccgaact ggtttttcat gattcggccc
    12721 agcagactat gcagcgcaat cagcgccggt tcgctgtcca gacgttcacc gtatggcggg
    12781 ttgctcaaca ctgtaccgta cggccctttc ggcagcggat tggtcagttg cgcgacatct
    12841 ttcacctcaa aggtgatcag ttcaccaatc cccgcaagac gggcgttagt gcgcgcacgt
    12901 tgaatcaccc gtgcgtcgct gtccgaaccg taaaaatgag agctatactc agccaggcct
    12961 ttacgggcgc gagtttgcgc ttccgctttc acttcctgcc agatagcttc atcatgctgc
    13021 gcccagccgc taaagcccca acgcccacgg tgcaagcctg gtgcgcgatc ggtcgccagc
    13081 atcgccgctt caatcagcaa cgtaccggaa ccacacatcg gatcgagcag cggtgttcct
    13141 gactgccagc cggatcgcat cacaatagcg gctgccaggg tttctttgat cggcgcaata
    13201 ccagcacgat cgcgatagcc acgcagatgt aaaccatcac cactcagatc aagagcgata
    13261 ctggcggttt ctttatgcag ccagacgtta acgcggatat ccggcgcatc gcgatcaaca
    13321 tttggacgcg gcagattttt ccgcgtgaaa gcatcgacga tcgcgtcttt cactttcatc
    13381 gcgccgtact gactgttgcg gatggtgtca ttcaaaccac tgaagtggac agcgaaggtc
    13441 gcgccaggat taaacatctc tgtccagttg atcgcctgaa caccgagata gaggtctaaa
    13501 tcgctgtaaa ccttacactc gcccagcggc aacataatac gcgaggccag gcggctccac
    13561 atcaggctct ggtaaacaag ccgtgtgtcg cccttgaaat ggaccccacc ctgaaccacc
    13621 tggcattcaa cggcccccag attttccagt tcagttttta acagctcttc cagcccacgg
    13681 gccgtactgg caaacagaga attcatatcg tcacttttac tctaagagcc taccgcagta
    13741 ggcgtaattg ttgcagtcag tctggacatg gagagcgcgg agaaaccgga gcgtacacaa
    13801 gtacgtgagg atttcgagca caacccaggg ccaaactgac aaataaaata gcctaacggg
    13861 acaggcgcta agaaaattgt tgcgcattat agctaataag cgcgtcatgt cataaagttg
    13921 agggcttatt ttcatttgag gaccgcaccg tggcgacatt aacccggctt tttattcatc
    13981 ctgttaaatc gatgcgcggc attggtctta cacatgctct ggctgatgtc agtggtctgg
    14041 ccttcgatcg catctttatg atcacggaac ctgacggtac gtttattacc gctcgccagt
    14101 ttccccagat ggtacggttt actccttcac ctgtgcatga tggcttgcat ctcaccgcac
    14161 cagatggcag tagcgcatat gttcgttttg ctgatttcgc cacacaagac gcaccaaccg
    14221 aagtttgggg cacacatttt accgcgcgaa ttgcgccaga cgcgatcaac aaatggctaa
    14281 gtggattttt ctcccgcgaa gtgcaattac gctgggtggg gccacaaatg acccggcgcg
    14341 tgaaacgcca caacactgta cctctgtcat ttgctgatgg ctatccttac cttcttgcta
    14401 acgaagcctc gttacgtgat ctccaacaac gttgtccggc cagtgtaaaa atggagcaat
    14461 tccgcaccaa tctggtggtt tccggcgcgt cagcttggga agaagatagc tggaaagtga
    14521 ttcgcattgg tgatgtggtg tttgatgtgg ttaaaccttg tagccgctgt attttcacca
    14581 ccgtcagccc agaaaaaggg caaaaacatc cggcaggcga accattaaaa acattgcaat
    14641 ctttccgcac tgcccaggat aacggcgatg tcgattttgg tcagaattta attgcccgta
    14701 atagcggcgt gattcgcgtt ggcgatgagg tggaaattct ggcaacggct ccggcaaaaa
    14761 tttacggcgc agctgccgct gatgataccg ccaacatcac gcaacaaccg gacgcaaatg
    14821 tagatattga ctggcaggga caggcatttc gtggaaataa ccaacaagtg ttgctggagc
    14881 aattagaaaa tcagggaatt cgtatccctt attcttgccg cgcgggcatt tgtggaagtt
    14941 gccgtgttca gcttttagaa ggcgaagtca cgccgctgaa aaaatcagca atgggcgatg
    15001 atggcaccat tctttgctgt agctgtgtac cgaagactgc ccttaagctg acgcgttaga
    15061 ctgcctgttc gaggctgaag ctgtcaacat tcacctgcgg tttcagcctg tcgttcatga
    15121 ttttaatggc gtcgcccaac tgcatcgcgc gacccgcaat aacaacgcag ggttgtgcca
    15181 gcaggcatag cgcggcattt tcgccagatt caacgaccag caaattgacc tgctcgtgag
    15241 tatcgctcaa ccaaacgcag gccgcatcgc ccggcatggg cacccacatt tcaccatggg
    15301 agacaaaatg ccagcttttc ggcatttgcg gtttgagata acgaattgct accagtgcat
    15361 tcagcaccag ttcggctttt tggtctttag ataaattaaa atcgcggcac ttttcttcaa
    15421 aagagaaata gagcgcggca tcatcaacac aaaagcctgc gggagaaaag gcatctggag
    15481 tcaacatttt gcgcgcaaaa cgtgagcgaa atagcatacc attggctaaa tcgagcatca
    15541 ttcgatcgtg ctcttcatcg taataccaac gccagttatc gtctggttta attcgcatct
    15601 gcctctcccc taccctataa aaccgcagca acacaatcca ttgtcgcttc gcttaataac
    15661 ctcaacaata aaagtacaaa atgtctaaat aagcaacaac gatgaatata aaacaaccag
    15721 ggctgaaaat aaagccctga ttatcttata aagaggaatt cgaagaaata attagatatg
    15781 ggtaacgatt tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac
    15841 cagtgaggca cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac
    15901 accgatgatc ggtaagcgac cgtttaattc tagtgacaag cggcgaataa tttctgtgct
    15961 ttttaactga agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg
    16021 aaccagagaa cgatcgagag tggtattggt tgcaataacg ccatcaatat tatggcgaac
    16081 taaactatcg gcaacctgga tcaattcgtc ttcagaaaga tccggcgcga tcttcactgc
    16141 gatcggcaca tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt
    16201 taagagatca tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga
    16261 tgaaatattg atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata
    16321 gtcatctttg ccctgctcca ctggcgtatc tttatttttg ccgatattaa tacccaagac
    16381 gccgtcataa tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa
    16441 gcccatacgg ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt
    16501 accaggctgt ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc
    16561 taacgcatca atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt
    16621 aaacgtcagg cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc
    16681 aaacggtgtt ccggtaatgc ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg
    16741 atcgagctgg aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc
    16801 ggtgtgcaaa ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg
    16861 caaaaaaaaa gcaaacgttt tcagaacctg tttcagggca gattttgctc cttctttgca
    16921 ctcaacccgc cgtaatcatt tatcgtccgc caggcgacat gacacggtgc tattacctga
    16981 caccagtttg tctgacgctg gcttttaggc ggcacgactc ccgcattact taacgcttta
    17041 ccatttacgc tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca
    17101 ccctgcgaat tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa
    17161 ccttcgggtc gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt
    17221 tcaactttgc cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca
    17281 ggcaagatgt cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg
    17341 agaataagtg gcggagcggc cagtaatagt gctggcactg tcgacgcttc ccccccccgg
    17401 cccaacgcgt tgggcgattg attttgctgt taataagcca agattcctga ctggtattag
    17461 tcagtaaaat agatatcgat tctctgtctg ccggaaagat aaatcgcgtt ccgccgacca
    17521 ccactcctgc ctgcaattca cccgccacca gcagcgataa aacggcgaaa atcaagagcg
    17581 ctaatctgtt ccacgtatta gtcataatga agtacaaatg tcgcgctggc attcacttta
    17641 cccgactgca catcgctatt gacaggtcgc aattgcgcat aaaaagttaa tggcacctta
    17701 ccgctatgta ggctgtagtc tttactcgcc tggttaagcg gaatcaatat cttcgcatca
    17761 tccagaatcg caattgccag cccactggct gcatccgtac cactttcaat ggacagcatt
    17821 tcgctctgca ctccgtcggg ggttcctgaa aaggtgacat caaccgtgct gacatctgtt
    17881 ccgcaatcct gcaaattaat gatgaaagat ttctccgggc cataattccc atgaaaaaaa
    17941 cgtgtcagcg agacatcgcc taactgcaca ttgatttgct tgctatcctg tgccagcacg
    18001 catgtattac ggcgaaacga tccgctaatg ttgaccgtgc catcatgggc aaatagctca
    18061 cagccccccg ttaatcccag taagaacaga atccagatta tccgtttcaa catcactcct
    18121 tcactggtaa accagatcaa aatagagaac cgccgtcgca ttaccgcccg ttgctcccgc
    18181 ctttgtggac ttataacgaa gttgatattt gagtgtgtta tccccggctt ttaagggcgt
    18241 aagaggctgg acctgattga gcgggatttc ttgctgactt tgcgcatcaa gaatttgcac
    18301 ggcaatcccc gttgccatac caccgcttcc gcctgtgtca gttagtttga gcagtgtcgg
    18361 cgcaagggtg tcagcctcgc cgctaaaaag gacgtccgcg ccagtaatac cagcggcaca
    18421 accggtgata tcaatattca aatcagcagc agtgcttacg gtattcgcgg cctgaaaatc
    18481 actgatatta aaataaccaa tatgaatatt ttgtaatgca cttttggtgg cgacatcaca
    18541 gccctgatcg accacattcc cataaatatg catactgact tgttcaggcc cagccatccc
    18601 gacatggatg ttaagaaggc taaagaacaa aataacgcac tgatatatcg tttttttctt
    18661 catacgttca cgcgttaatc aaaatttatt ctcaccgttg ccgtggcttc aaatcgcccg
    18721 gtttctggtg gtacgcctgt cgtgctgact ggatagacat tcattcgcgg atactgtctg
    18781 catcatcagg aatagagagc ggtagtgtcc caccagcagg ggaaataatg ttgttctggc
    18841 tatcggtcac caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg
    18901 ggtctgaggt tgccgtcaga ctcagattga cgttaactaa ccctgcgttt cccgtgttac
    18961 acctgaccgg aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac
    19021 gaaagttagc cgcaaatacg tcgcccagat caacctcgat tgtttctccg gcattgacgg
    19081 aacatgagcc aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtaccag
    19141 tagtcgtgca ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca
    19201 taggaggaat gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc
    19261 tattaccgct agccccctgg ccacctaaac gtagaccgcc gggatcgcaa atatgccatc
    19321 gtgtatctgc ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa
    19381 cctgcaataa aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat
    19441 agactaacgg tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc
    19501 aatcacagga aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta
    19561 ctccagccgt attttcttct tcatccagag ttttaacgcc aaaattgacg ccaatatacg
    19621 gattcccctg agtggtatga cactgcccga tataagcagc agtttgagcg caccatagca
    19681 ttaagacggc ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct
    19741 gcatatggca tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact
    19801 gatatgacaa atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg
    19861 caaaccggac atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc
    19921 gtccagcgtc gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa
    19981 tacccttgct cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac
    20041 cacgttgact actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc
    20101 gattcgattt tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt
    20161 tttcagcccg gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac
    20221 gttagtttcc cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc
    20281 tgaaagagcg tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg
    20341 ggcatctgca aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc
    20401 gctgttatat ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt
    20461 taaacccagg ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca
    20521 ggcgtaattt tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg
    20581 cagggaaaaa tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta
    20641 gttccaggaa acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag
    20701 ccgatcctgt tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacaaacc
    20761 gtactcgcct aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa
    20821 attgtaataa cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc
    20881 atccatatgt ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata
    20941 gcccatcaat tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata
    21001 tgactgacct ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa
    21061 cgaaattgct ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata
    21121 ttcagcatat tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt
    21181 ggtctgggca aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt
    21241 caccgcgtat ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct
    21301 gtagctattg acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga
    21361 cgtggaatag agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt
    21421 gtaaccattt tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg
    21481 cgcaaaccct ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg
    21541 aaagctgaca ctatcgaaaa aatcgcctgc agtataatca tcacctacgg tcagttcgcc
    21601 ttttagtgga ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg
    21661 gccactactg cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc
    21721 actgtttaaa ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact
    21781 gttagccccg ctaaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc
    21841 acgggcatta cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac
    21901 attaaacgtt acgctggcat ccggaataat ctccgccaat ggcacacaca cttgtttgtc
    21961 cagttgttta aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt
    22021 cgtcaaacag gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt
    22081 atctacaatt gtctgattca gaacaa
//
LOCUS       DADLCQ010000062        21569 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000062 DADLCQ010000000
VERSION     DADLCQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.062"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..219
                     /locus_tag="JQG95_002231"
     CDS             <1..219
                     /locus_tag="JQG95_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5888772.1"
                     /translation="SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIE
                     YYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            321..473
                     /gene="hokA"
                     /locus_tag="JQG95_002232"
     CDS             321..473
                     /gene="hokA"
                     /locus_tag="JQG95_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY5888773.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(660..872)
                     /gene="cspA"
                     /locus_tag="JQG95_002233"
     CDS             complement(660..872)
                     /gene="cspA"
                     /locus_tag="JQG95_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY5888774.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1153..1443)
                     /locus_tag="JQG95_002234"
                     /pseudo
     CDS             complement(1153..1443)
                     /locus_tag="JQG95_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1877..2587
                     /locus_tag="JQG95_002235"
     CDS             1877..2587
                     /locus_tag="JQG95_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY5888775.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="JQG95_002236"
     CDS             complement(2637..3611)
                     /gene="ghrB"
                     /locus_tag="JQG95_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY5888776.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3715..4365)
                     /locus_tag="JQG95_002237"
     CDS             complement(3715..4365)
                     /locus_tag="JQG95_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY5888777.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4528..6861
                     /locus_tag="JQG95_002238"
     CDS             4528..6861
                     /locus_tag="JQG95_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY5888778.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6830..7270)
                     /locus_tag="JQG95_002239"
     CDS             complement(6830..7270)
                     /locus_tag="JQG95_002239"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5888779.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7267..7830)
                     /gene="tag"
                     /locus_tag="JQG95_002240"
     CDS             complement(7267..7830)
                     /gene="tag"
                     /locus_tag="JQG95_002240"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY5888780.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7988..8686
                     /locus_tag="JQG95_002241"
     CDS             7988..8686
                     /locus_tag="JQG95_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5888781.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYVALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8915..10123
                     /locus_tag="JQG95_002242"
     CDS             8915..10123
                     /locus_tag="JQG95_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888782.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10447..12138
                     /gene="eptB"
                     /locus_tag="JQG95_002243"
     CDS             10447..12138
                     /gene="eptB"
                     /locus_tag="JQG95_002243"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY5888783.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12230..12306
                     /locus_tag="JQG95_002244"
     tRNA            12230..12306
                     /locus_tag="JQG95_002244"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Pro,seq:cgg)
     gene            13050..14657
                     /gene="dppA"
                     /locus_tag="JQG95_002245"
     CDS             13050..14657
                     /gene="dppA"
                     /locus_tag="JQG95_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY5888784.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14965..15984
                     /gene="dppB"
                     /locus_tag="JQG95_002246"
     CDS             14965..15984
                     /gene="dppB"
                     /locus_tag="JQG95_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY5888785.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15994..16896
                     /gene="dppC"
                     /locus_tag="JQG95_002247"
     CDS             15994..16896
                     /gene="dppC"
                     /locus_tag="JQG95_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY5888786.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16907..17890
                     /gene="dppD"
                     /locus_tag="JQG95_002248"
     CDS             16907..17890
                     /gene="dppD"
                     /locus_tag="JQG95_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY5888787.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17887..18891
                     /gene="dppF"
                     /locus_tag="JQG95_002249"
     CDS             17887..18891
                     /gene="dppF"
                     /locus_tag="JQG95_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY5888788.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18921..20192)
                     /locus_tag="JQG95_002250"
     CDS             complement(18921..20192)
                     /locus_tag="JQG95_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5888789.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20668..20775
                     /locus_tag="JQG95_002251"
     CDS             20668..20775
                     /locus_tag="JQG95_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY5888790.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21151..21258
                     /gene="ldrD"
                     /locus_tag="JQG95_002252"
     CDS             21151..21258
                     /gene="ldrD"
                     /locus_tag="JQG95_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY5888791.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         5199 a         5303 c         5701 g         5366 t
ORIGIN      
        1 tccagaaaag gcaattgtct ggataatgct gtggtggagt gtttctttgg aaccttaaag
       61 tcggagtgtt tttatcttga tgagttcagt aatataagcg aactgaagga tgctgttacg
      121 gaatatattg aatactataa cagcagaaga attagcctga aattaaaagg tctgactcca
      181 attgaatatc ggaatcagac ctatatgcct cgtgtttaac tgtccaactt tttggggtca
      241 gtacaaagtg aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa
      301 atcaacacag ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt
      361 gtttcacgct tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta
      421 aacaggagag ttatgagctg gcggcgtttt tagcctacaa attgaaagag taagagtctt
      481 cggcgggaaa ttattcccgc cttacttacg gcgttgcgca ttctcattgc acccatattt
      541 attcttcaca aaaataataa tagattttat tacgcgatcg attatttatt tcctgaaaaa
      601 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
      661 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      721 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      781 aacacatctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      841 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      901 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      961 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
     1021 cgtcaaaccg ttgatgtgca ttaagccact cattggcggg tgatgcaaca attatttttc
     1081 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
     1141 gtggataaaa gtctatttca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
     1201 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
     1261 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttca
     1321 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
     1381 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
     1441 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
     1501 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
     1561 tcagcctgtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1621 tcttatcact cctgttttgt aaagatttcg caaatctaac cttctgcgaa ggcgtaaatc
     1681 tctggctaca aaaaaggcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1741 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1801 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1861 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1921 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1981 agcgtttatc gacttcctgc aaaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     2041 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     2101 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     2161 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     2221 tggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     2281 gcactctgcg ctaaaacaga gcgatgacct gaaaccggta tttgatcagg cattcaccaa
     2341 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     2401 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     2461 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     2521 gccattaccg gcacaacatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     2581 ccaataaaga gtaaaagccc gagcgattat cgggctttac ttcacgcagt cgcagcttag
     2641 tccgcgacgt gaggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2701 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2761 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2821 tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct gcaatgctgc gatcagtgca
     2881 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2941 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     3001 acgaaatctg actcttgtaa cagagtatcc aaatcgcagt agcgggcgtt gaagcgttct
     3061 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     3121 acacgttgtg ccagcgccat gccgatccgt cccatcccga caatgcccag tgttttatgg
     3181 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     3241 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     3301 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     3361 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     3421 attttttcca gcaatgcggc atctacattc tcgtttgaac ccagtaaacc ttcagcttct
     3481 gcaaaaattg ctgcattttg aacgacggtt tgtgggctga ggtttgccac ctggtgaacg
     3541 gtgaaatgcg cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3601 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3661 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3721 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3781 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3841 ggtgatcaac gcgctggcaa cggaatccgc acgctgctgg gagagacgca tgttcaggtc
     3901 gtgacccccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3961 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg gagcgctgct
     4021 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     4081 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     4141 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     4201 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     4261 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     4321 taccgccaga gcgccactca ctacggcggc aagtaagata aacacgtttc ttcattgtta
     4381 atccttaata acctttttat tctttgtcac gggttccgtg gcgggagatt atgccgcgtg
     4441 aacatgaaga ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat
     4501 acatctaacc tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg
     4561 actgccgccc actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg
     4621 cgtggcgcgt tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt
     4681 cacagcaata cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa
     4741 aacccgcaag gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg
     4801 gatcttattc accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt
     4861 gctggttcct acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa
     4921 cgctatatgg cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg
     4981 gcacaggcga tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt
     5041 tggccgctgg tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat
     5101 acgctgaaaa ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg
     5161 cgtgacagtg ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc
     5221 tttggcgata aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg
     5281 gggatcgccc atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc
     5341 accacaggtt atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa
     5401 aacgccgaat gggcggcaga gatttgtggt gttggcgcag cgaaaatccg cgagctggcg
     5461 gctattttcc accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag
     5521 tttggtgagc aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc
     5581 acacccggcg gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg
     5641 cgttctgcgg tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat
     5701 aaaatccctg ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac
     5761 aacggtatga accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac
     5821 tttactcatc atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg
     5881 gtgatctctg aatgcttctg gacggcggcg gcgaaacacg cggatattgt tctgcctgcg
     5941 actacctctt ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg
     6001 gtgccgatga agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt
     6061 gccgagttga gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt
     6121 cagctgcaat ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag
     6181 gttgaattgc cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa
     6241 aacccggaca gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat
     6301 ccgttaaaaa ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt
     6361 tacccggatt gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc
     6421 gaaccagaac agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg
     6481 aattacagtt ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat
     6541 cctgacgatg ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc
     6601 cgtgggcaga tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc
     6661 attcacgaag gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc
     6721 gcagtgaacg tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt
     6781 aatacgacgc tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt
     6841 gaaccaccgg ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat
     6901 cgacaatgtg aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa
     6961 cctccagcat cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac
     7021 cgcgtctgac ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc
     7081 tgacaaaacc gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat
     7141 aggcatcccg caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc
     7201 cccaggttgt actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt
     7261 cccgaatcat ggtttatttc ccagatagca acagcagcca accacatgat cattcaccag
     7321 cccacatgcc tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt
     7381 tttcagtgcc ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt
     7441 tgtggcttgt gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cgggaaacgg
     7501 ttcgccgttc tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt
     7561 ccctcgatgg cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat
     7621 tgctgcgacc ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt
     7681 gaggacggtg atccacgata acccagcctg ctgcccttca aagcagatca tttcgaacag
     7741 ttttttactg tcagtttcag gcacgcccca ctcattatca tggtaggcaa tataaagcgg
     7801 gccctgactc acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa
     7861 aatttcagcc gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact
     7921 atgcccgata tccttcatac aatgacaata atattgccga attcggctct tccctggagt
     7981 cgtgttaatg atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt
     8041 ggtaatcagt gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga
     8101 tacgcaaccc ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta
     8161 caacgatatt ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa
     8221 tctggcggaa gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc
     8281 tttatatgaa caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac
     8341 ctcgatgtat aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc
     8401 cagcgtgagt agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc
     8461 gcaaatcagc tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag
     8521 ccggatgacg gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat
     8581 gttgctcaat caaaatattg ccgcctatgt tgcgctaact caggcagaaa ataccactaa
     8641 taatagcgac tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa
     8701 ttgaaaccat tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa
     8761 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8821 tggcatttca tgccgttttt ccccaagcat aaaatgcact tcgttatggt tgtcggcaga
     8881 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8941 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     9001 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     9061 tttctctttc ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt
     9121 gcaggaacgt tttggcgtta aacgcgtcac cgtggcttcc ggcattttgc tgggattagg
     9181 cttcttcctg accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct
     9241 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9301 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct
     9361 gggtttcaaa tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt
     9421 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9481 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9541 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9601 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9661 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9721 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9781 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9841 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9901 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9961 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
    10021 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
    10081 gccagagcag aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta
    10141 ctgggcttca gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt
    10201 aaaatttggg ccagttaaca ggcccttttt actgcttttt gtaacgctaa agtctctttt
    10261 caaacttgca tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct
    10321 ttgctgtggt ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt
    10381 tcccctgccg ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt
    10441 gtttgcatga gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc
    10501 tatattggcc tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac
    10561 gattttaccg tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg
    10621 accttctttt tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg
    10681 tcgctggtgg tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc
    10741 attggttatg gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt
    10801 gttggtctga actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc
    10861 tggaataacc gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt
    10921 cgcagcctgg tggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg
    10981 gatatccagc agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt
    11041 gtcgtagcga actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg
    11101 gcgcgggtcg atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca
    11161 taccaggctc cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg
    11221 cgttgggacc atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag
    11281 gagaaaaatc tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg
    11341 cgttgcatgt ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa
    11401 cagaacattt tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag
    11461 agcgaaatgt ggttctacag caacacgatg gcagacaaca ttgcttatcg tgagcagatt
    11521 ggtgcggagc cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg
    11581 cagcaatcgc tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt
    11641 tcgcatttta actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt
    11701 attggtgttg atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg
    11761 acctatgtgg atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt
    11821 gtgttctacg cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg
    11881 ccgcgtgaac tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat
    11941 aaatatctgg aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac
    12001 atgaaagtgc cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat
    12061 acttcaccgg atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa
    12121 gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta
    12181 ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc
    12241 gcagcctggt agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac
    12301 cgaccaaatt cgaaaagcct gctcaacgag caggtttttt tgcatttgta gctcatgagg
    12361 atgagaacct ccgggggcag ggaggctcga ctcgacgtag gcctgataag acgcgccagc
    12421 gtcgcatcag gcaacggcac cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac
    12481 caacacacca cacattttcc tgccaacttt gcgccccata tctcactatc cattaacgga
    12541 tttgtgacag aaaccattgt ctttttttat atttgcttaa atcttttttg cgttacttat
    12601 ggctggcgct ggcattttcc gctgtgcgtt ttagcgttca tggtattaat cgctcagata
    12661 tccacctttt cctcagattt ttttcattaa atgcataaaa cttaatcacc aaatgttgct
    12721 aaatattaag ctgattgttc tggttatgtt ggagtagcac aaatttctct gctgcaaata
    12781 gctgtttgaa gtttttttaa tctttgtttg tggattctca ccgttggtgt aaatcccggt
    12841 tggctgtatt gacgttttca cattctgttg acagattgta ggtcacgagg ggcattttat
    12901 ggaggatccg cactgttaca ctgatgttaa ttagtacggc atccccacct cataacgttg
    12961 acccgaccgg gcaaaaaaca aaaaaggtca ggcagcgaca acccactgca aagggttaaa
    13021 acaacaaaca tcacaattgg agcagaataa tgcgtatttc cttgaaaaag tcagggatgc
    13081 tgaagcttgg tctcagcctg gtggctatga ccgtcgcagc aagtgttcag gctaaaactc
    13141 tggtttattg ctcagaagga tctccggaag ggtttaaccc gcagctgttt acctccggca
    13201 ccacctatga cgcctcttcc gtcccgcttt ataaccgcct ggttgaattt aaaatcggca
    13261 ccaccgaagt gatcccgggc ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct
    13321 ataccttcca tctgcgtaaa ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc
    13381 gcgaaatgaa cgccgatgac gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc
    13441 cgtaccataa agtttctggc ggcagctacg aatacttcga aggtatgggc ttgccggagc
    13501 tgatcagcga agtgaaaaag gtggacgata acaccgttca gtttgtgctg actcgaccgg
    13561 aagcgccgtt cctcgctgac ctggcaatgg acttcgcctc tattctgtca aaagaatatg
    13621 ctgacgcgat gatgaaagcc ggtacaccgg aaaaactgga cctcaaccca atcggaaccg
    13681 gtccgttcca gttacagcag taccaaaaag attcccgtat ccgctacaaa gcgtttgatg
    13741 gctactgggg caccaaaccg cagatcgata cgctggtctt ctctattacc cctgatgctt
    13801 ccgtgcgtta cgcgaaattg cagaagaatg aatgccaggt gatgccgtac ccgaacccgg
    13861 cagacatcgc tcgcatgaag caggataaat ccatcaacct gatggaaatg ccggggctga
    13921 acgtcggcta cctctcgtat aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc
    13981 aagcactaac ctacgcggtg aacaaagacg cgatcatcaa agcggtttat cagggcgcgg
    14041 gtgtatcagc gaaaaacctg atcccgccaa ccatgtgggg ctataacgac gacgttcagg
    14101 attacaccta cgatcctgaa aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag
    14161 gtttctccat cgacctgtgg gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc
    14221 gcatggcgga gatgattcag gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca
    14281 cctacgaatg gggtgagtac ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga
    14341 tgggctggac tggcgataac ggggacccgg ataacttctt cgccaccctg ttcagctgcg
    14401 ccgcctctga acaaggttct aactactcaa aatggtgcta caaaccgttt gaagatctga
    14461 ttcaaccggc gcgtgctacc gacgaccata acaaacgcgt tgaactgtac aagcaagcgc
    14521 aggtggtgat gcacgatcag gctccggcac tgatcatcgc tcactccacc gtgtttgaac
    14581 cggtacgcaa agaagtcaaa ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa
    14641 acgtctctat cgaataatta aaagccatac aagactgatg gcaaaggcaa aactgcctga
    14701 tgtgctccgc ttatcaggcc tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt
    14761 aggccggata aggcgttaac gccgcatccg gcataaacaa agcgcacttt gtcaacaatc
    14821 tgtatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    14881 tctgtccgac aagggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    14941 gagtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    15001 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    15061 gatccggtga tgatcatggc aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg
    15121 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    15181 gtaatgcacg gcgatctagg catttcaatg aaaagccgaa ttccagtatg ggaagagttc
    15241 gtgccgcgct ttcaggccac gctggaactt ggcgtctgcg cgatgatttt tgctacggca
    15301 gtcggtattc cggttggtgt gctggctgcg gttaaacgcg gttccatttt cgatcacact
    15361 gcagttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    15421 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    15481 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    15541 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcccgc cattgtgctg
    15601 ggtactattc cgctagcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    15661 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    15721 gtccatgcgc tgcgtaacgc gatgctgccg gtagtgaccg ttatcggcct ccaggtggga
    15781 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctggggcgc
    15841 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    15901 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    15961 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    16021 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    16081 aaaggcgcgg tcgtcgggct ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc
    16141 aactggattg cgccttataa cccggcggat cagttccgcg atgcgctgct cgccccgcca
    16201 gcctggcagg aaggcggcag tatggcacac ttgctgggca ccgatgacgt cggtcgtgat
    16261 gtgctgtcgc ggctgatgta cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg
    16321 gtgttgtcgc tgattatggg cgttattctc ggcctgatcg ccggttactt cggcggcctg
    16381 gttgataaca ttatcatgcg cgtggtcgat atcatgctgg cgctgccaag cctgctgttg
    16441 gcactggtgc tggtggcgat tttcggcccg tcgattggta acgccgcgct ggcgctgacc
    16501 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat
    16561 cgcgattacg tcaccgcctc tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt
    16621 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    16681 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    16741 gagtggggca ctatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    16801 accttccctg gtctggcgat cctgttgacg gtgctggcat ttaacctgat gggtgacggt
    16861 ctgcgtgacg cgctcgatcc caaactaaag cagtaagagg ttcgagatgg cgttattaaa
    16921 tgtagataaa ttatcggtgc atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg
    16981 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    17041 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    17101 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    17161 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    17221 ctacaccgtg ggtttccaga ttatggaagc gattaaagtg catcagggcg gcaacaaaag
    17281 tacccgccgt cagcgggcga ttgacctgct gaatcaggtc ggtattcccg atccggcttc
    17341 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    17401 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    17461 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    17521 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagctgcgc ataaaatcat
    17581 tgtgatgtat gcaggtcagg tagtagaaac gggcgatgca cacgccatct tccatgcgcc
    17641 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    17701 acgtctggcg tcgttgccag gtgttgttcc cggcaagtat gaccgcccga acggctgcct
    17761 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    17821 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    17881 cacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    17941 aaaaaacatt atccggtgaa gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg
    18001 gacggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    18061 tgcggtaaat cgaccctcgg tcggttgctg acgatgatcg aaacgcccac cggcggcgag
    18121 ctgtattatc aggggcagga tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg
    18181 cagaaaatcc agatcgtctt ccagaatccc tacggttcgc tgaatccgcg taagaaagtt
    18241 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    18301 gaaaaagccc tgtcgatgat ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    18361 ccgcacatgt tttccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    18421 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    18481 caggtgctga atctgatgat ggatttgcag caggagttgg ggctttctta tgtctttatc
    18541 tcccacgacc tgtcggttgt ggagcacatt gctgatgaag tgatggtgat gtacctaggc
    18601 cgctgcgtgg agaaaggaac gaaggaccaa atcttcaata acccgcgcca tccgtacacc
    18661 caggcgctgc tctccgcgac gccacgcctg aacccggacg atcgtcgtga acgcatcaaa
    18721 ctcaccggtg aactgccaag cccgctgaat cctccgccgg gttgcgcctt caacgcccgc
    18781 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggaggt
    18841 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    18901 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    18961 atgccgtaaa gcagaatcag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    19021 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    19081 tgaaagaaca ccagcactga tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata
    19141 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    19201 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    19261 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    19321 gggatcacct gcgcggccag tgccagtgcc gagatatttt gttcaaaggc agaaacggct
    19381 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    19441 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatctgct
    19501 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac cgcagaaaag
    19561 aagcaaaatg gaatggtaag caggacatcg cggaaaaaga cggaggcttg cgggaaggcg
    19621 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    19681 actttgacca ccaccattgg cccagaaatc ttaaacagta atcgttcacc accagacgca
    19741 atcgctatca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    19801 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    19861 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    19921 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    19981 gtttttaaat aaatgtcctg cactacccag gtgacaggat aagcaatgat cgctgcggta
    20041 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    20101 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    20161 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    20221 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    20281 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    20341 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    20401 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    20461 aagcaatcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
    20521 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    20581 tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat agccgcgaaa
    20641 cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat ggccttctgg
    20701 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    20761 aacaagcgga agtagcgtgt catgtgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    20821 gaccaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
    20881 agcggataat attctgcgga atgcgcagca cagagtgaag atgagacgga ttccggaaca
    20941 gaaaaacagt cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg caatgttgcg
    21001 ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    21061 atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag aatagccgcg
    21121 aaacgttgtc attacaatac aggcggctat atgacgttcg cagaactggg catggccttc
    21181 tggcatgatt tagcggctcc ggtcattgct ggtattcttg ccagtatgat cgtgaactgg
    21241 ctgaacaagc ggaagtaacg tgccatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    21301 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    21361 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagacgtgac ggattccgga
    21421 acagaaaaac agtcgtagaa tcagcgtcaa gccggaaggg ttccggtgag gcgcaatgtt
    21481 gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    21541 ggtatgcacc tgacaacatc acgggggcc
//
LOCUS       DADLCQ010000063        21480 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000063 DADLCQ010000000
VERSION     DADLCQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.063"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(71..418)
                     /gene="grxD"
                     /locus_tag="JQG95_002253"
     CDS             complement(71..418)
                     /gene="grxD"
                     /locus_tag="JQG95_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY5888792.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            753..1580
                     /locus_tag="JQG95_002254"
     CDS             753..1580
                     /locus_tag="JQG95_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY5888793.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPHTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1708..2289
                     /gene="sodB"
                     /locus_tag="JQG95_002255"
     CDS             1708..2289
                     /gene="sodB"
                     /locus_tag="JQG95_002255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY5888794.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2435..3604)
                     /locus_tag="JQG95_002256"
     CDS             complement(2435..3604)
                     /locus_tag="JQG95_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888795.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3770..3859)
                     /gene="ynhF"
                     /locus_tag="JQG95_002257"
     CDS             complement(3770..3859)
                     /gene="ynhF"
                     /locus_tag="JQG95_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY5888796.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4158..5183
                     /gene="purR"
                     /locus_tag="JQG95_002258"
     CDS             4158..5183
                     /gene="purR"
                     /locus_tag="JQG95_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY5888797.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5180..6112)
                     /locus_tag="JQG95_002259"
     CDS             complement(5180..6112)
                     /locus_tag="JQG95_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5888798.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6225..7436
                     /locus_tag="JQG95_002260"
     CDS             6225..7436
                     /locus_tag="JQG95_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY5888799.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7727..8875
                     /gene="cfa"
                     /locus_tag="JQG95_002261"
     CDS             7727..8875
                     /gene="cfa"
                     /locus_tag="JQG95_002261"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY5888800.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8915..9556)
                     /gene="ribE"
                     /locus_tag="JQG95_002262"
     CDS             complement(8915..9556)
                     /gene="ribE"
                     /locus_tag="JQG95_002262"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY5888801.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9771..11144
                     /gene="mdtK"
                     /locus_tag="JQG95_002263"
     CDS             9771..11144
                     /gene="mdtK"
                     /locus_tag="JQG95_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY5888802.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11185..12441)
                     /locus_tag="JQG95_002264"
     CDS             complement(11185..12441)
                     /locus_tag="JQG95_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888803.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12749..12825
                     /locus_tag="JQG95_002265"
     tRNA            12749..12825
                     /locus_tag="JQG95_002265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12783..12785,aa:Val,seq:gac)
     gene            12830..12906
                     /locus_tag="JQG95_002266"
     tRNA            12830..12906
                     /locus_tag="JQG95_002266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12864..12866,aa:Val,seq:gac)
     gene            13014..13319
                     /locus_tag="JQG95_002267"
     CDS             13014..13319
                     /locus_tag="JQG95_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY5888804.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13445..15049
                     /locus_tag="JQG95_002268"
     CDS             13445..15049
                     /locus_tag="JQG95_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY5888805.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15061..15873)
                     /locus_tag="JQG95_002269"
     CDS             complement(15061..15873)
                     /locus_tag="JQG95_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888806.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIAGGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15877..16662)
                     /locus_tag="JQG95_002270"
     CDS             complement(15877..16662)
                     /locus_tag="JQG95_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY5888807.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16659..17327)
                     /locus_tag="JQG95_002271"
     CDS             complement(16659..17327)
                     /locus_tag="JQG95_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5888808.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17391..18029)
                     /locus_tag="JQG95_002272"
     CDS             complement(17391..18029)
                     /locus_tag="JQG95_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY5888809.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18042..19235)
                     /locus_tag="JQG95_002273"
                     /pseudo
     CDS             complement(18042..19235)
                     /locus_tag="JQG95_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012082.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(19530..19862)
                     /locus_tag="JQG95_002274"
                     /pseudo
     CDS             complement(19530..19862)
                     /locus_tag="JQG95_002274"
                     /inference="COORDINATES: protein motif:HMM:NF014750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde:ferredoxin oxidoreductase"
     gene            complement(19835..20143)
                     /locus_tag="JQG95_002275"
     CDS             complement(19835..20143)
                     /locus_tag="JQG95_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde:ferredoxin oxidoreductase"
                     /protein_id="HAY5888810.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVAMPIWVAFLQRK
                     "
     gene            complement(20164..20790)
                     /locus_tag="JQG95_002276"
     CDS             complement(20164..20790)
                     /locus_tag="JQG95_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY5888811.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21246..21455)
                     /gene="fumD"
                     /locus_tag="JQG95_002277"
     CDS             complement(21246..21455)
                     /gene="fumD"
                     /locus_tag="JQG95_002277"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY5888812.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5369 a         5567 c         4989 g         5555 t
ORIGIN      
        1 gaggttttac gacgcaccgg ataaaggact gacggatgcc cgttcgcatc cgtcagtatt
       61 gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca gtttctttga
      121 tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa ccgccgacca
      181 gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc ggcagttccg
      241 cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca catgcggcaa
      301 gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa cctttcatgt
      361 acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg gtgctcattg
      421 tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt cagtgcggca
      481 tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct atttgttgca
      541 tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata accattttta
      601 gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa caataaaagc
      661 tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc agacgcaggc
      721 atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt atttcgatca
      781 cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat gcttcaaagc
      841 aagccaggga gagttctgct accactcata tcaccaaaaa agcagataaa aagaaaagca
      901 cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa gccgccagta
      961 aaagtatgac caaaagcaaa accgcttctt ctgttaaaaa atcttccatt accgcttcta
     1021 aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc agctttaccg
     1081 aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa aatgccgcgt
     1141 caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa gtggcaatga
     1201 ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca ccgcataccg
     1261 gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa attcgtattc
     1321 cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa cgtagtgagc
     1381 tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc gatcatgtcg
     1441 gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag gaaattcaaa
     1501 tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt cgggtaatga
     1561 ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt aagttcatct
     1621 tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta ttgtacgtat
     1681 gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact accatatgct
     1741 aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta cggcaagcat
     1801 catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt tgaaggtaaa
     1861 tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc agctcaggtc
     1921 tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga accgactgga
     1981 aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa agcgcagttt
     2041 actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa aaacagcgat
     2101 ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac cgatgcgact
     2161 ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg caatgcacgt
     2221 cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc gaaaaatctc
     2281 gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt accatgccaa
     2341 cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc agcaactgta
     2401 acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg caaactgttt
     2461 caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg acaatcgccc
     2521 ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca ccagcagctg
     2581 ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac agacctggcg
     2641 cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca aaggttgctg
     2701 cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg aacgggattg
     2761 ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta actgaacgat
     2821 ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc agcaccagca
     2881 ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga gagatatagg
     2941 tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca gacagcacct
     3001 gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct gtaccacctt
     3061 taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc gttgccagaa
     3121 atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg ccaccgatat
     3181 ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta tgttttggca
     3241 ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc aggctggtca
     3301 aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga acgttgccta
     3361 gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga gaaagtagaa
     3421 gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt aacattccgg
     3481 cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc attggcgaga
     3541 actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga tagttaattt
     3601 tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt ggcgaaagca
     3661 tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg ccattttgct
     3721 aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat cagtgcagcg
     3781 cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt accagtgaaa
     3841 atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa aagtgatatt
     3901 acgcattttt acacactgtg atgaaaaaat ctcccgtcat ttataatgat aagtgttttt
     3961 accacttccc cttttcgtca agatcggcca aaattccacg cttacactat ttgcgtactg
     4021 gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa cacttttgtt
     4081 gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc tgaagttagg
     4141 gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca aacgtttcca
     4201 ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa acgcgcaacg
     4261 ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg cgtagcctga
     4321 aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg gcctattttg
     4381 ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc ctgattctgg
     4441 gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg gcgcaaaaac
     4501 gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg gcgatgctgg
     4561 aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa gctgacttca
     4621 ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt tatctgattg
     4681 aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac accggcgcag
     4741 gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg ccggaaagct
     4801 ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag caaatcctgt
     4861 cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca atgggcgcac
     4921 tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg atcggttatg
     4981 ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat cagccaaaag
     5041 attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac aaacgtgaag
     5101 aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg gctgacggcc
     5161 cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc tcccgtagcc
     5221 actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt aacgcaggcg
     5281 acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg ttttccagtt
     5341 caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt ggcaccatcc
     5401 ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt acgacaagtc
     5461 ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag gtgtcttcgc
     5521 gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc atcaacgcca
     5581 gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc cggaaggcat
     5641 aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg cgcccgtcgg
     5701 aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttcg acatcatcaa
     5761 aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca atattatcca
     5821 ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc tggcgggtga
     5881 tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca gcagcggtca
     5941 gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct tccagctgac
     6001 gcacggtata gctgaccgca gaaggaacac gatgcagctc ctgtgccgca gcgctaaaac
     6061 taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac atagtctgcc
     6121 tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa tttcactccc
     6181 tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa cctgggaaaa
     6241 gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc gatatgtatc
     6301 tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct gtcagtgcca
     6361 gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg ccactctccg
     6421 accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg ttaggtagtc
     6481 tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt gtacaggctg
     6541 tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat tatccttcac
     6601 agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct ccggcactgg
     6661 ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt ttcgccaccc
     6721 tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc acgacgaagg
     6781 cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct aaaacctatc
     6841 gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg ctgaccggtt
     6901 caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta agttatgtcc
     6961 cgcaaactat cgcgtttctg attggtggtt atggctgtcg cgccgcactg cagaaatggc
     7021 aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc attgcgacct
     7081 gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca ttctgtgtga
     7141 tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg cgtcccttcc
     7201 cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt ctgtgcttcc
     7261 tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc accaccacca
     7321 gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa cgttgtgaag
     7381 aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca cactgatcta
     7441 tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg aaattagcgg
     7501 cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac ggggacagga
     7561 tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc cctctgttct
     7621 acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc caaaaagcgt
     7681 ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga gttcatcgtg
     7741 tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg aattacttag
     7801 ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga aaaaccccga
     7861 tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt atatggatgg
     7921 ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg caggtctcga
     7981 gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc gtctcttcaa
     8041 tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt tgggtaatga
     8101 cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact ggaaagatgc
     8161 cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa aattgcagtt
     8221 aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactag cgcactacat
     8281 ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac agcaaaaaat
     8341 ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt atcgtgacct
     8401 gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg gaccgaaaaa
     8461 ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta tattcctgct
     8521 ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta ataaatatat
     8581 ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg aaccccactt
     8641 tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga tggcgtggta
     8701 tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac gctttaaacg
     8761 gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg atattcagct
     8821 ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc gctaaaggct
     8881 attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct ggttgattca
     8941 tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc tgagtttgtg
     9001 gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc gtgcgttcca
     9061 gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg acggtcaggc
     9121 taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac tgactatcct
     9181 ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc acttcagcgg
     9241 tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat ttcgccgcac
     9301 gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg cgtaacgttt
     9361 ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc aggcagcaac
     9421 cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg ggtaactcca
     9481 ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg gtgccctgta
     9541 caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg ttcagcacaa
     9601 tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt ttcactggag
     9661 aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt ttgctgccca
     9721 ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac gtgcagaagt
     9781 atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc gcgcaaatcg
     9841 cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt gccaccgaca
     9901 tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt ggtcacggac
     9961 tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga cgtgagcgca
    10021 ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt ctcattatgc
    10081 tggtgctgtg gaatgcaggt tacattatcc gctacatgga aaacatcgat ccggctctgg
    10141 cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga tatctgttct
    10201 tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt atggtaatgg
    10261 gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat ggtcatttcg
    10321 gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg tattgggtca
    10381 tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat attcgtaacg
    10441 aaaaaggcac cgctaagccc gagcctgcgg ttatgaaacg actgattcaa ctcggtttgc
    10501 cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct ctgttagtgt
    10561 ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt agttcactga
    10621 tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt tatcgtctgg
    10681 gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg gtgggtgtct
    10741 gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc gccctgttgt
    10801 acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg gcggcggtat
    10861 atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt tataaagata
    10921 cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg ccaagcggct
    10981 atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc ttctggatag
    11041 gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg cggttcctgc
    11101 aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga caaggcgcaa
    11161 ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa tcccgtcttc
    11221 gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg ttatctcaac
    11281 gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa tttctttcgc
    11341 actggtttgc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca gttgcacatg
    11401 cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt taccaacaat
    11461 gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac cgccctgctg
    11521 tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg aagcgccgtc
    11581 cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta cgacaaaatt
    11641 ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta ccgcattttc
    11701 cagcgtaccg gcatgcactt cagcccgccc ccccacattc accgacaggt tttgcgtctt
    11761 accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc cattctcaac
    11821 aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa gcaacgtatc
    11881 actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat tgctggcttc
    11941 tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg aggttgtccc
    12001 attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat gtccttccag
    12061 tacacgcagg ttaccgccat tttccagcaa caaaccgcag gcataaccct gatcaacgct
    12121 gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac taattgcgcc
    12181 tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata gcgtgccatt
    12241 gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc agccagcccc
    12301 cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc cttcagtaag
    12361 ttgcgtcgcg ccaaggacct cgccggtctt aacaatttgc acattcccgt cgctcatcaa
    12421 gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc tttattgcta
    12481 cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt ggcaaacaac
    12541 tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg cgatcctgaa
    12601 taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc tctgcaaacg
    12661 gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc tgccgctaat
    12721 attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta gagcaccacc
    12781 ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg cgtccgtagc
    12841 tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt ccactcggac
    12901 gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct ctgtatccaa
    12961 tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc gctatggcaa
    13021 ctttgttaca acttcatttt gcttttaatg gcccatttgg cgacgcaatg gctgagcagc
    13081 ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag gtatggacag
    13141 aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa aaaagcgccc
    13201 ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag gaagttgtcg
    13261 ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa ctcgcctgac
    13321 agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt ctcaaattct
    13381 aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt acaggtaagt
    13441 aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca ccttattctc
    13501 gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg acgaggccgg
    13561 tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa atattgccag
    13621 tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc aagaagccag
    13681 ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt ttttaccgcg
    13741 aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc aggcacgaca
    13801 gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc aaattacaaa
    13861 tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg atttagcggt
    13921 gattgccacg ggtcacgtct ggcctgatga agaagaagca accagaacgt attttcccag
    13981 cccgtggtca ggcctgatgg aagcaaaggt cgatgcgtgt aacgtgggta ttatgggaac
    14041 atccttgagc ggactggatg cggcaatggc agtggctatt cagcatggtt cgttcattga
    14101 agatgataaa caacacgtcg tttttcaccg cgataacgca agtgaaaagc taaatattac
    14161 gttgatgtcg cgcacgggta ttttacccga agccgatttc tattgcccta ttccctacga
    14221 gcccttacac atcgtcaccg atcaggcatt aaatgctgag attcaaaaag gtgaagaggg
    14281 ccttttggat cgggtattta gattgatagt agaggaaatc aagtttgctg atccagactg
    14341 gagccaacgc atagccttag agagcctgaa tgtcgattcc tttgctcaag cctggtttgc
    14401 cgagcgcaaa caacgcgacc catttgactg ggcagaaaaa aatctccagg aagtcgaacg
    14461 caataaacga gaaaaacata ctgttccctg gcgttatgtc attctgcgcc tgcatgaagc
    14521 cgtacaggaa attgttcctc atctgaatga acacgaccat aaacggttca gtaaaggcct
    14581 tgccagggtt ttcatcgata attatgcggc aatcccttca gagtctattc gtcgactact
    14641 ggccttacgt gaagcgggga tcattcatat tctcgccctc ggtgaagact acgaaatgga
    14701 aattaatgag tcgcgcaccg tcctgaaaac ggaagacaac agctactcgt ttgacgtttt
    14761 tattgatgcc cgcggacagc gtccgcttaa agtgaaagat attcctttcc ctgggctacg
    14821 cgagcaatta cagaaaacag gggatgaaat ccctgatgtt ggcgaagatt atacgttaca
    14881 gcaacccgaa gatattcgtg gacgcgtagc gttcggcgcg ttgccctggt tgatgcacga
    14941 ccagcctttc gttcagggac ttacggcatg tgcagaaatt ggtgaggcga tggctcgggc
    15001 ggtcgtaaag cctgcatccc gtgctcgtcg gcgtctttcg tttgattaaa ggtaaagcta
    15061 tcagcactca acaaactgat gctgttcatt aagacgatag cggactccgg ttcgaatatt
    15121 gttttcacct tcaatggcga cggcgaagcg aactctttcg ccgtcatgat atgccagtgc
    15181 ggcgcttccc cctagcccga gaataattga gtcgaccacg cccgtgctca ctaccgttga
    15241 attttcaccg ccggcaatga tgtgtacatt gtcgccgctg ttggcaattc tggcgtttgc
    15301 gccaaagctg gcgatttgca ccaggtcacc attactggcg acatgacacc gttcacccaa
    15361 tgtacaaacg cgtactcgca ttccggtatt cgcaatccga ctactatctc cagcgctgct
    15421 tatccgcgaa gaattaccgg ttacagcaac ccgtgcgcgt tcaccggagc tgccaatgtg
    15481 actgttaaag ccgacacttc caatccgcac gctataaccc atactggcaa tttgcgcggc
    15541 atacccagcg gttgcgattc gggcgttatc ctcacaacag ccgatgcgag catgatcgtc
    15601 gctgcgggca aactgactgc aaaatggtat ctctccgggg tctgtggcca gtttttgcat
    15661 agaggaaact tcctgatgcg cgaagttttc atccgccagc cattttttcc acgcatactc
    15721 aaccagactc tccgcccagt ccgtatatcc ttcctcaatc agtgcatgac gaatatcggc
    15781 ataactaccg ccgcgtggga aatggcgcag gaaccagcga tacatcaccg ctccgatacg
    15841 ccattctcgc aggtctgctc gtgtaatgat cattcgttag tgccggtgat agccatcgac
    15901 catgctttta aaggtttcgt gtggcgtacg ccccgtggtg caaagataaa gatgaccgaa
    15961 gataaaaaag aggcttataa atgccagagc aaaatgcgcc tgcaataacc agtatcttac
    16021 gccaggaaac acatctccca cggcttgcgg atagagacac aacagccccg tcaatagtaa
    16081 caacggcagc aatccataca tgacaccaac ataggcgacc tgctgtaagg gattaaattt
    16141 agactgtgtt gttgccggga aaggatgttc ttccccctgc ataatgccaa acaaataaaa
    16201 tcgcgtttgt tttgccgctc gttccagcca cccctgacga cgaatgcgat agtggtgacc
    16261 attatcccca acggcattga tcagcacaaa gccgagccag catgccagta acaaaaatcc
    16321 gcaaacttca tgcaccgcaa ccagactttt aaccgcagtt gcgcccacca tcgcaaagtg
    16381 atttatcagc ccacttgcca gcaacaatac aaagagtaac gcattcgacc agtgccataa
    16441 tctgaccgct ttgctgtaaa gataaacctt ctcaccatga tcggtggctg atttcttcac
    16501 gccacgagca cgaagcaagg cgtgcagccc cagcaccaac cacatcccaa ccagcaatac
    16561 tccggcaatg atcaaccaca ccggccaaaa ttcgggagtg aactgtggca catagttcgc
    16621 taactggctc tgaaactgtt cagcatgttg cgacgggttc atacattttc ctttttaatc
    16681 aaatgttgac cgaaccggcg atacagatgc ggttttccgg caccaggtag ctgatattga
    16741 tagtatttat tatcctgcaa ccacgcctga atttctgggc tatcttcgcg accaaagatc
    16801 aatgcgtgtt ccggacaggc gctgacgcaa atgggcggaa agccttttgc cagtcgggac
    16861 tcagcgcaga aatcacattt atctgccact ttagtgacag ggttaaggta gcgaacctgg
    16921 tacggacaag cgccaatgca ataactgcag ccgatgcact gcgatttttc tacccgcacg
    16981 atcccctgct catcacgcca cgatgccccc gtcgggcata cgtcgatgca cggagcatct
    17041 tcgcagtgct gacatgactg gcgaaaaaag tgatactgcg tttcgttgtc gttatcggtg
    17101 actggaatgt gtgctatcga taaacggctt ccctgagcgg ggacgtggtt agttttacgg
    17161 catgcgcggg cgcaaatatt acagccattg caccgtgact cgtcatgaat catggcatac
    17221 cgaacttcct tttcttgcct cgtgttcgct aacagagaag acgcagaacc agtaaaaaag
    17281 attactgttc ccatccccag aacaaatttg cgtcgagtaa aggacatctg attatcccct
    17341 aatgcagtgg ctgcaactgt ccaccctatc catgacatct ccctgaaatt tcaggggttc
    17401 tgaaactgct ctacctcgaa ccattcgcat aagccataag ccttgccata ggtcataaat
    17461 cgctcgctaa acaagtacaa caccgccgtc gatgactcca cctgccgtat atcggcatat
    17521 tcaggtgcaa catccatcgc ggcgatcgcg gcttcaatct gcgcttcctg gaagtaatat
    17581 ggcgcttgca tcagcatggc gactttgtaa ggacgaggat acgtctggca ctcaaaacga
    17641 acggcgtgtg caatggcgcg acaaatgtcc tgctccacaa cttgcaaaga catcgccgcg
    17701 tagttttcac tcatggcctg cgttgagtaa tagtaatcat cctgacttcc cgtcatacaa
    17761 gcgatatccg cgtagtctcc gttctgctta atgctttcca gcaaaactga cagttcctct
    17821 tctgcaaccg aatagggtgg cgagagaaac acggtgcgcg ccaccagctg cccagccgca
    17881 gaatgctggc gaatgaactc agccaggatt tcagcaggtt ctggttcggt taccgtatca
    17941 acggggtgac gcatcccctg caaccattgc cgcttcgcct cgtcaacttc gctgactttc
    18001 gctaacggca attcatcctg atggttcatc ttacccattc cttacgcagg cagtagattg
    18061 tgtgctgcca gatcggcggc aatatcttcc agccccagac gctgcaatgt ttcgcgggtt
    18121 ggacaaccaa gctgtgggtc ccagcccatt tctttgtaga acatcgtgag cgaagcatgc
    18181 atatcgtcac gatccatttt gtcagtacct tcagtaaaga ccgggatctg cggatccttg
    18241 tcgaaaaccc aggaacagat aagatcgtgt tcattacgca tatctttggt ttgcatcggt
    18301 tttaccgtgt aggcacgatg caacgtaaag atacgctctg cagctaaatc taatttttcc
    18361 tgagtcatct cttcgccggt gatcgctttg aagaatttgg cttaaagcgc cagatcgccc
    18421 cggtaattac ggcttttcag cggcgaaacg gtcattggcc agacccagtt gcacagagtg
    18481 acggcgttat gcaaacagac ccgcaacagc gaccactttg catatttaat ttttgcgtca
    18541 ttgattggcg tgtagttttt ggtttcatcg taagcatctt cagaaccaaa aagttctttc
    18601 gccacttcac gttgcagttt caatggcaag ccggaaccaa taaagttgat atgggtatgc
    18661 gtcatgcagt cacggttgaa catacagtta acaatggaac cgacctgcgc tgacgcttca
    18721 ttggcatggt gaaccggata gccaaacggc gaccagagtt tatttttcgc gtagccccag
    18781 tactctttac ccaaattcca gcgttctgcg atggcatatg aaccatcagc caggtgactc
    18841 agctcaccca cacgatgcgc cagacggtag taaaaatctt taatgaagtt aacgtcaccc
    18901 gcttccagtt gatcccagcg aatttctgca tactcttcag ctggcagaac acgcttgaac
    18961 acacctttgc tgtaacaata agtaaagtcg cgatgcaact gcccgtagtt acaccatagg
    19021 ccgtagtcat cgaacaaatt cagaccaacc aggttaccaa tcacacgacc atcgtcttta
    19081 tcttcaaaat ctttcggccc gttcgggaag atggtggtat ggacaaagtt tgctacacaa
    19141 gtgttaccac ctgtgctggg cacgccaaac tctttcaccc gaggaatatt catttgggtc
    19201 atacaacgga tcgggcaaga gtggcagccg ctcattttta ctgtgtattt ctctgccgcc
    19261 ggtcctaagt caaaaacgga tttataggta cgaaagccga ccgtattctg attgcctggc
    19321 ggaatttcac ccgtttcaat cggaccacct tcagccgcgc cccaaaacag ccctttacgt
    19381 gctgtccagc gtgacttggg atctgaatac tctgcccacg attgtggcgt acttggcacg
    19441 acatggttgt tattcgcacc aataagttca gtcatcatgt aatcattgag acgcttcatc
    19501 tcctgacgat cggcaatgtt gacccctttc gtcccttcaa ccgcaatcgc tttcaggttt
    19561 ttcgaaccca ttattgcgcc agttcccgca ccgccgctgt ggttacggct attcaacatg
    19621 ccagagagag gaataaggtt ttccccagcc tgaccaatag ccgccacaca ggtttccggg
    19681 ctggtcaatc gacaaatttc ttccgtcgtt gcgcgcgtcc cttttcccca taagaaatcg
    19741 gctttttcca ggctaacttt gtcatcttta atcttcagcc ataccggtga tttcgccttc
    19801 ccttcgataa taatgacgtc gtatccagcg aatttcattt gcgctgcaaa aaagccaccc
    19861 atatgggcat cgcgactaaa tttcctttgg taaaagtaga aagtgaggtg atatttacgc
    19921 gagaactaca gggggcacca gatccagtta atgggccggt agcaaagact aatttattcg
    19981 cttcatcgaa aggtttcgtg cctagcggta cttcgtcata cataattttg tagccgaagc
    20041 ccatgccacc gacaaaactt ttaaacttac tggaatcttc gagggtaata tttcctgtcg
    20101 tgagattgac tcttaatata ttacctgtcc aaccgttagc catgattttt tcctttgcaa
    20161 gattcacaca gtaatatctt tccactcgat aatttttaac gcccctgttg ggcaggcgtt
    20221 tgcgcattca ccgcataaca cacatttcga ggatttttta ctttcggtat ttacggtggc
    20281 catcatccac gggcatgccg tagtacaggc gctacagcca atacaacgtt tatgatcgac
    20341 ggtaatacag ccttctttct gctgccaggt aatcgcacca atcgggcaga cgttcatgca
    20401 ttgcggctct ttgcattgac ggcaggtgtc cgcggtatag ttgagatcgc catacaggcc
    20461 gccgccagag ccaaccccgt tgtcgccaaa gaaataattg cgatggattt tgatacggga
    20521 gaagaatgtc cctactgagc catcgttgaa gttggtacaa gagatttcac agcggtggca
    20581 acccgtgcat cgcgctcgct gggtcaccag cacccctttg ggtgtattta tcaaccctac
    20641 tgtgccgcta tcaatatctt cctgtttgca acccaaaagt gacagtaacg caggggcaat
    20701 tgttagacca gcaaggcctt ttcctgatat acgcaagaat tctaatcgcg ttaagccaat
    20761 atctaatagt ggacgatcaa ccgggttcat ttatttcgca tcctcttcgc aaacacagtg
    20821 ctgacaattc agcagcctga tattaagcat tcaactaata cgctgctgac tctgtcccca
    20881 acaccagata ttttccctaa cccgatagtg tatgagaaaa gatttaacgc cgataaacta
    20941 atcctttatg gttaggagaa aaaataatgt gattattctg agcccttaac attgatcgtt
    21001 atcaattaaa attacaaagc aagaaattat aagcgaactg atatttatta tcatttgaaa
    21061 taaatttaac ttaagcgaca atcatttgtg acgttcattg cagaaaataa aaataacatt
    21121 atcgctataa attaaaatac ataccgaaat tcgtataatt tcagcagttg ttatttagaa
    21181 tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg cctcatcctg
    21241 aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc cagctcactg
    21301 cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac aatatgcttt
    21361 ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct gcacatttct
    21421 cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt atcatgccaa
//
LOCUS       DADLCQ010000064        21418 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000064 DADLCQ010000000
VERSION     DADLCQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.064"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..134)
                     /locus_tag="JQG95_002278"
     CDS             complement(<1..134)
                     /locus_tag="JQG95_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888813.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     "
     gene            complement(236..1216)
                     /locus_tag="JQG95_002279"
     CDS             complement(236..1216)
                     /locus_tag="JQG95_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY5888814.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1224..1934)
                     /locus_tag="JQG95_002280"
     CDS             complement(1224..1934)
                     /locus_tag="JQG95_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY5888815.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2151..3974)
                     /gene="typA"
                     /locus_tag="JQG95_002281"
     CDS             complement(2151..3974)
                     /gene="typA"
                     /locus_tag="JQG95_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY5888816.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4347..5756
                     /gene="glnA"
                     /locus_tag="JQG95_002282"
     CDS             4347..5756
                     /gene="glnA"
                     /locus_tag="JQG95_002282"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY5888817.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5929..6978
                     /gene="glnL"
                     /locus_tag="JQG95_002283"
     CDS             5929..6978
                     /gene="glnL"
                     /locus_tag="JQG95_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY5888818.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6990..8399
                     /gene="glnG"
                     /locus_tag="JQG95_002284"
     CDS             6990..8399
                     /gene="glnG"
                     /locus_tag="JQG95_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY5888819.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8511..8621
                     /gene="yshB"
                     /locus_tag="JQG95_002285"
     CDS             8511..8621
                     /gene="yshB"
                     /locus_tag="JQG95_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY5888820.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="JQG95_002286"
     CDS             complement(8850..10223)
                     /gene="hemN"
                     /locus_tag="JQG95_002286"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY5888821.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFEEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="JQG95_002287"
     CDS             complement(10412..10921)
                     /gene="yihI"
                     /locus_tag="JQG95_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY5888822.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11503..12135
                     /locus_tag="JQG95_002288"
     CDS             11503..12135
                     /locus_tag="JQG95_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY5888823.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12517..15303)
                     /gene="polA"
                     /locus_tag="JQG95_002289"
     CDS             complement(12517..15303)
                     /gene="polA"
                     /locus_tag="JQG95_002289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY5888824.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15305..15547
                     /locus_tag="JQG95_002290"
     CDS             15305..15547
                     /locus_tag="JQG95_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888825.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            15667..16599
                     /locus_tag="JQG95_002291"
     CDS             15667..16599
                     /locus_tag="JQG95_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY5888826.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(16640..18070)
                     /locus_tag="JQG95_002292"
     CDS             complement(16640..18070)
                     /locus_tag="JQG95_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY5888827.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="JQG95_002293"
     CDS             complement(18225..18851)
                     /gene="dsbA"
                     /locus_tag="JQG95_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY5888828.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="JQG95_002294"
     CDS             complement(18868..19854)
                     /gene="srkA"
                     /locus_tag="JQG95_002294"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY5888829.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19931..20200)
                     /locus_tag="JQG95_002295"
     CDS             complement(19931..20200)
                     /locus_tag="JQG95_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY5888830.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20270..20854
                     /gene="mobA"
                     /locus_tag="JQG95_002296"
     CDS             20270..20854
                     /gene="mobA"
                     /locus_tag="JQG95_002296"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY5888831.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20836..21363
                     /gene="mobB"
                     /locus_tag="JQG95_002297"
     CDS             20836..21363
                     /gene="mobB"
                     /locus_tag="JQG95_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY5888832.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5238 a         5377 c         5149 g         5654 t
ORIGIN      
        1 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
       61 gacggtaatt tataaattgt accgccacac agtgttaata gactaaataa cgcccatttc
      121 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
      181 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
      241 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
      301 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
      361 gtaggggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
      421 agcggaacgc cgacgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
      481 gctcatcatc gccaaggcag ataaggtgcg ttaataacgc tttgaatggc atatcaccct
      541 gacctgaaat acaggcttta tgacccaggc caccctgttc tttaacgatc aaggcatctt
      601 taatatggac ctgggtaata tgcggtgcca tcgtttttaa ggcgtcgatg ggatgctcat
      661 ttgcattgat catgttagca aaatcaaaca gtaaggaaag agactccatc tcactttctt
      721 tgaccagcga caccagctca tgacttttta aatcttcatg ctgctcaatc gtaaaagtca
      781 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgata atcgataata
      841 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgttgcc ccagttttca
      901 acgcgatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
      961 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     1021 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     1081 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     1141 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     1201 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     1261 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     1321 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     1381 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     1441 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     1501 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     1561 agttcagttg atggcgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta
     1621 cggtttttct cggtaaaccc ccaagaaggc tctcgccctt gctcaattgc ggcccgctga
     1681 aagctcgccg acagctccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     1741 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     1801 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     1861 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     1921 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     1981 gccaaaaaag aaagcaatta acggtgtatg actttgcaga taattgtgac aaagctctct
     2041 attgacgtaa tcacagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     2101 aaaaaaaaac ctgccatcgc tggcaggttt tttatgacta aaaaacgaaa ttaatcgtct
     2161 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     2221 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     2281 agagtcatgc ggataggcgg aaccagaaca acagcttcgt cagtaccgga agcacgcatg
     2341 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctatgaata
     2401 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     2461 tgcagaccga acagcgcgaa cgcgacagct ttaccctgac cgttagagat cagtacaccg
     2521 ttctgacgct gacccacttc gcccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     2581 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     2641 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     2701 tcgcctttac gttcgcccag cgcctgcatc acagaaccct gatgctgttc ttcaacgtcc
     2761 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcta tttcacggaa gattactttc
     2821 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgataag aacagagagg
     2881 tgcagttcac cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     2941 agcgcaacgt tgtgtaccag ttctttgttc agacgatcga ggatctgacg agacgttacg
     3001 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     3061 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     3121 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
     3181 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttacgggtt
     3241 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc acgcttgatg
     3301 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     3361 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctgatacagc
     3421 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     3481 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     3541 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     3601 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     3661 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     3721 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     3781 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
     3841 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     3901 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     3961 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     4021 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     4081 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
     4141 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
     4201 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     4261 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     4321 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     4381 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacaacacg
     4441 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     4501 gctcctcgat tggcggctgg aaaggcatta acgaatccga catggtgctg atgccagacg
     4561 catccactgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     4621 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
     4681 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     4741 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     4801 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     4861 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata
     4921 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     4981 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     5041 aagctgacga aattcagatc tacaaatatg tcgtgcataa cgtagcgcac cgcttcggta
     5101 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     5161 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     5221 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     5281 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctacgaa gcaccggtaa
     5341 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     5401 cgaaagcacg tcgtatcgaa gtacgtttcc cagatccggc agctaacccg tacctgtgct
     5461 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     5521 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     5581 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     5641 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
     5701 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     5761 tgaagttgta ctacccggcg cacaacgccg ggatttagtt gccgtggaaa ctttcagccc
     5821 atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt ttatccgtaa
     5881 aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat ggcaacaggc
     5941 acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt gttaattgat
     6001 gacaatctgg cgatccatta cgccaacccc gccgcgcaac aactgctcgc ccaaagctcc
     6061 cgcaaattgt ttggtacgcc gttaccggaa ctgttgagct acttctcatt aaatatcgag
     6121 ctgatgcaag aaagtctgga ggcggggcaa ggctttaccg ataacgaagt gacgctggtc
     6181 atcgatggtc gctcgcatat cctttctgtg acggcccagc gtatgccgga cggcatgatc
     6241 ctgctggaga tggcaccgat ggataaccag cgccgcttaa gccaggaaca actacaacat
     6301 gctcaacaag tagctgcccg tgatttagtg cgcggcctgg cacatgagat taaaaatccg
     6361 cttggcggtt tacgtggcgc ggcgcagtta ctcagcaaag cgttacctga cccatcacta
     6421 ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaattt ggtcgaccgt
     6481 ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa agtggctgaa
     6541 cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat tcgtgattac
     6601 gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt cttgctgaat
     6661 attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat tctgcgtacc
     6721 cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc gcggattgat
     6781 gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt ttacccgatg
     6841 gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgcccgtaa tttgattgat
     6901 cagcattcag gcaaaattga atttaccagt tggccaggtc ataccgagtt ctcggtttac
     6961 ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg gtagtcgatg
     7021 acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggagcgggt ttaacctgta
     7081 cgacatttga gaacggcgcg gaagtactgg aggcgctggc gagcaaaacg ccggatgtgc
     7141 tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag cagattaaac
     7201 agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg gatgctgccg
     7261 tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat atcgacgaag
     7321 cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag ccgcgtaata
     7381 ttcagcttaa cggcccaacg accgatatca tcggcgaagc gcaagccatg caggacgtgt
     7441 tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac ggcgaatccg
     7501 gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc accaaagcgc
     7561 cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca gaactgtttg
     7621 gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt tttgaacagg
     7681 ccgatggcgg cacgttattt ctcgacgaaa ttggtgatat gccgctggat gtgcagacgc
     7741 gtttgctgcg cgtgctggca gacggtcagt tttatcgcgt tggcggctat gcgccggtga
     7801 aagtggatgt gcggattatc gctgccactc accagaatct tgaacagcgg gtgcaggaag
     7861 gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat ctgccgccgc
     7921 tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag gttgccgcgc
     7981 gcgaactggg cgtagaagcg aagttgctgc atccggaaac cgaagctgct ctgacgcgtc
     8041 tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta acggtgatgg
     8101 ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa tcaacggttg
     8161 cggagagtac ttcgcaaatg caaccggaca gttgggcgac acttttagcg cagtgggcag
     8221 acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca gagctggagc
     8281 ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa gcggcgcggc
     8341 tactcggctg gggccgcaac actctgacgc gtaagttaaa agagctgggg atggagtgat
     8401 tcacggcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt tgcgctttac
     8461 tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt atgctggaat
     8521 caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa actgccgttg
     8581 ctgccgtgct gtgtgccgcg atgattgggc tgtttagctg aagctcgttg tttatgccgg
     8641 atgcggagaa agtcgtaggc ctgataagac gcgaccagcg tcgcatcagg catcatgctc
     8701 agatgccaga tgcggcgtaa acgccttatc cggcctacaa aagcaataat ttcaacaggt
     8761 tttgttgccc agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc
     8821 tactttgtaa acgaagcgcc attcactctt taaatcaccc gtgagaactg ctgcatccgc
     8881 gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct
     8941 ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg
     9001 gcgagcagct tgagatcttc ctcaaagtaa tcagcgaagt gcaaatccca ctgtttctca
     9061 agaggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata
     9121 cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc atacacttgc
     9181 tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg
     9241 gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta
     9301 cgatgcagca cgccttcacg ctgggccacc gccagctcgt catcaggacg ggcaaagtga
     9361 tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata
     9421 tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa
     9481 atggtcggca gatgcgcgta gttaaaaacg ctcagacgat cggggttcag ctccgccaca
     9541 cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg
     9601 atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct
     9661 tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg
     9721 ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc
     9781 ggatcgactt cgatcgaaat ctccgcatcg gtattgaaat ggaagttttc gcgcagcagc
     9841 ttcatcagac ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc
     9901 aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc
     9961 gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag
    10021 aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga
    10081 tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg
    10141 gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc
    10201 cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc
    10261 gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg
    10321 acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca
    10381 gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt
    10441 cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct
    10501 gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca
    10561 gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta
    10621 attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg
    10681 tgactttttc agtcactccc aatggaatag gggttttact gccaatacgt ggatcttttg
    10741 gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg
    10801 catgaccacg acgttttttc tgacgcttac ggtcacgagc ttcctgatcc agctcctcgc
    10861 gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca
    10921 taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa
    10981 gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct
    11041 taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttccat tagccggaac
    11101 aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat
    11161 cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg
    11221 tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca
    11281 gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata tagcttattt
    11341 gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca
    11401 aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca
    11461 ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat
    11521 caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga
    11581 attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg
    11641 actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac
    11701 ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa
    11761 gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt
    11821 cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat
    11881 cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa
    11941 gcggacaaac tggcaagcgg cgcacgtaaa gcgctattga atatggtgcg tgaagctgta
    12001 ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgta
    12061 gacaagctga ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg
    12121 caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg
    12181 taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac
    12241 ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa
    12301 tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct
    12361 gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt
    12421 ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt
    12481 gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc
    12541 gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg
    12601 atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc
    12661 gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc
    12721 aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc
    12781 acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg
    12841 gcgtccgtcc agcgtttcaa cgtagcccag ctctttcgcc tgagcacggg tgcgttccat
    12901 atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt
    12961 acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa
    13021 gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa
    13081 cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc
    13141 tttatcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac
    13201 aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg
    13261 caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc
    13321 ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc
    13381 ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg
    13441 atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt
    13501 tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact gcttggtgga
    13561 agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg
    13621 caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg
    13681 ttcaatgcgt gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg
    13741 ccctttgtgt ttctgcagat ccggccacat tttcagatgc aactgcaagg tgacatctgc
    13801 atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt
    13861 tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc
    13921 gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt
    13981 atcaaacgca atcccacgca gttcaatgcc gtaattcgcc agaataccgc gatcgtattt
    14041 caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag
    14101 tgcacgctcg tgagagattt gatcgggcgc atcaagataa tcatgagcaa ccggaatata
    14161 tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc
    14221 aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca
    14281 cgctttcagt gtttcttcat caaggatagt gacgtagttg tcataagaaa tcaccgttgc
    14341 cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctgtttttgc
    14401 ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata
    14461 ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca
    14521 ggtcagctcc agttcaacat cggttttaat cgtcgccaac tggtatgaga gataagcaac
    14581 ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc
    14641 aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg
    14701 cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcagaggagt cacccatcag
    14761 cgccaggaag tcgatgatca attctggcgg cacgccgtac ttattcacca cctcttccgg
    14821 tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc
    14881 catatcttta tcgccagtgc taatcagcac cggacgcccg gctttttcgg cttcgcgcgc
    14941 cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat
    15001 cgctttaacc atcgcgtgca gaggttcgat ttgtgctcgc agatcgtccg gcattggcgg
    15061 gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa
    15121 gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac
    15181 accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata
    15241 tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac
    15301 cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa
    15361 catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tctgctggat cgctcgcaaa
    15421 tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc
    15481 tttttacagc atgatgtttg tctttgtttt tcatgcagtt aaaaataaat ccatccctaa
    15541 taattgagat aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag
    15601 gatcatccgt tatttgggtc attttctgct aaaatctgcg ccttgcgctt ctttagtgca
    15661 ctgtctatgg ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc
    15721 cttttgctga gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc
    15781 attgccggga ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt
    15841 ttttgtgatt ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac
    15901 ccacacctgc aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg
    15961 cttatttgta accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag
    16021 cacattccga tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc
    16081 ctggcgtgct gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta
    16141 cgtcatccgg agcgacgtgg tcaagatgtt gaaaccactc ggcgttcttg tgaaaagttt
    16201 cgcctgcatc ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa
    16261 catcagcaaa cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg
    16321 atggcgctaa atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat
    16381 ccggacaata accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcggattgtc
    16441 gtacatgtgg atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa
    16501 agctttaagc gccattttca gcaatggctg aactcattgt ggcaggaaaa agaccggctt
    16561 ctgacgtctc tcatgtcatc acagcgtcag aataagtaag ttttactcgc ctctgaatga
    16621 gcagaggcga gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt
    16681 cgctcatctc ctgtccgtta aaaacaactt tacccggtgt ataacgcagc tgcagcgagg
    16741 cggtattgtt gtcaatcgtg attaactgaa acatctgacc taatgtcatc atcccgctga
    16801 tttgtttatc agcctgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct
    16861 gttttgcggt ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttga
    16921 tatctttgtt tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg
    16981 catttagttc gccgagtgcg ttcttccatc ttaccggttg tttaatggtc ggctcacttt
    17041 tttgtaataa cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt
    17101 catcgtttgc taactcaggg tgtgcagcaa gctgcttctg catcgcaatg ttatactgga
    17161 tgataaattg gcgcactgct gagggatcaa tagattcagc aatcaatgac cactcaccac
    17221 taccgagttg ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga
    17281 tataatcttt gtctttaacg cgatccagcc tggtttttgc aacgaaagca tcgatttggg
    17341 caacgtcttc cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa
    17401 ccggaaattt cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta
    17461 ctttgttcat tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt
    17521 tagacaatga actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaattttc
    17581 catactctgc aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca
    17641 atgtcgcttc cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat
    17701 tattcactaa gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca
    17761 ttgtgatggg caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct
    17821 tgatattgag atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa
    17881 aaaagccttt gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct
    17941 ttttcgcatc gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg
    18001 tgtaccatgt gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact
    18061 tacgtatcat ataaatattc cctgataaaa cagcagaact ttgaatatcc acttatgctg
    18121 aaataatgtc cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa
    18181 aaaagcccgt gaatattcac gggctttatg taatttacat tgaattattt ttcctcggac
    18241 agatatttca ccgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata
    18301 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacacc acgcaattgc
    18361 acatcagctg cggctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac
    18421 gccgcgtcgt actcttcacc tttaatacct gcattgataa atacatcgcg gatatcagat
    18481 gctgaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct
    18541 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca
    18601 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca
    18661 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa
    18721 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca
    18781 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa
    18841 atctttttca tgatctactc tctccgatta atacataggt gttaattgca aagggggttc
    18901 ttgtagaact tttgcctgtt ctataaaagt cgccgtctgt cgtagccagt aatcttcccc
    18961 ggttaaccac gggaaatttt tcgggaacgc gggatcagcc caatgccgca ttagccaggc
    19021 aagataataa accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc
    19081 gaactcgcta aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctgggc
    19141 tttatcgcca ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc
    19201 cagatcaaca aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg
    19261 tagccgcaga acggtgaaat cttcccgcca gtgtgctgta acggcggcga tcagctcatc
    19321 tgtcgctttc agaaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag
    19381 cttgcgaggc tcaatgagat attcattcag accgatggtc gggcgatgaa taaaaagctg
    19441 tttgcgcccc gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat
    19501 attatcagct tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg
    19561 atgattcaat aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt
    19621 taccagctgc aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg
    19681 gcgataaaat ttgacgacaa aacgtcgacg ctcttcgtcc tgaaattgat agacacggtt
    19741 ttcatagctg ttaagcgggg taagaccgga atccacccgg atcccttgct caaacagagc
    19801 gtccatgatg gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc
    19861 atccggaaaa cattacgaat gatgaaggat atcatttcac gatgattttg gtggcgccgc
    19921 ttacaagctt ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt
    19981 cttcataatc tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca
    20041 ggtgatagat cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt
    20101 tcgccagttt ctgcaaaaat tgcaacaagt taaagtctgg ctctttttgc caggctggct
    20161 ggaggagttc aataacttca ttcagacgtt tacatttcat agtaatgctc cttactcttg
    20221 agacagacac gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat
    20281 gacgacgata acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga
    20341 taaaggattg ctggacttaa atggcaaacc gctgtggcaa catgtcgctg acgcgcttaa
    20401 gacgcagctc tctcacgtcg tggttaatgc taatcgtcat caggaaatct atcaggcagg
    20461 cggtctgaaa gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct
    20521 ttcagtaatg cagcaggaag ctggtgagtg gtttttgttt tgtccgtgcg atacgcctta
    20581 cattccccat gatttagccg cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg
    20641 ggtccatgac ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg ctattgagcc
    20701 tttattactg gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc
    20761 tggcggtcat gcggttgatt tcagcgatca taaagatgca tttgttaacg tgaatacgcc
    20821 agaggagctt gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg
    20881 agtggtaccg gaaaaactac gcttttgaaa aaactgatcc cggcattatg cgccagaggg
    20941 atccgtccgg ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa
    21001 gatagctatg agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga
    21061 tgggccttga tgacggaaac accagacgaa gaagagctgg atctacattt tctcgcaagt
    21121 cgaatggata cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc
    21181 gcaaagattg tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac
    21241 aggcatgtta ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat
    21301 attaatgatg ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga
    21361 taagcaagag taggctggat cagacgcagc cagcgtcgca cccggcattt ttttcacc
//
LOCUS       DADLCQ010000065        21274 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000065 DADLCQ010000000
VERSION     DADLCQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.065"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..444)
                     /locus_tag="JQG95_002298"
     CDS             complement(<1..444)
                     /locus_tag="JQG95_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY5888833.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /locus_tag="JQG95_002299"
     CDS             complement(499..1350)
                     /locus_tag="JQG95_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY5888834.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /locus_tag="JQG95_002300"
     CDS             complement(1363..2163)
                     /locus_tag="JQG95_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY5888835.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQG95_002301"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="JQG95_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY5888836.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMVPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="JQG95_002302"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="JQG95_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY5888837.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="JQG95_002303"
     CDS             complement(5214..6812)
                     /locus_tag="JQG95_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5888838.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQG95_002304"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="JQG95_002304"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY5888839.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="JQG95_002305"
     CDS             complement(8491..9069)
                     /locus_tag="JQG95_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY5888840.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQG95_002306"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="JQG95_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY5888841.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="JQG95_002307"
     CDS             10508..10687
                     /locus_tag="JQG95_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY5888842.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11166)
                     /locus_tag="JQG95_002308"
     CDS             complement(10807..11166)
                     /locus_tag="JQG95_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY5888843.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="JQG95_002309"
     CDS             complement(11528..11872)
                     /locus_tag="JQG95_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY5888844.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="JQG95_002310"
     CDS             12004..13914
                     /locus_tag="JQG95_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY5888845.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="JQG95_002311"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="JQG95_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY5888846.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="JQG95_002312"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="JQG95_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY5888847.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15493..17178
                     /gene="fadD"
                     /locus_tag="JQG95_002313"
     CDS             15493..17178
                     /gene="fadD"
                     /locus_tag="JQG95_002313"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY5888848.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHALEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17260..18375
                     /gene="rnd"
                     /locus_tag="JQG95_002314"
     CDS             17260..18375
                     /gene="rnd"
                     /locus_tag="JQG95_002314"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY5888849.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLDVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="JQG95_002315"
     CDS             complement(18429..19394)
                     /gene="yeaX"
                     /locus_tag="JQG95_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY5888850.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGIPPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19450..20052)
                     /locus_tag="JQG95_002316"
     CDS             complement(19450..20052)
                     /locus_tag="JQG95_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY5888851.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(20409..20975)
                     /locus_tag="JQG95_002317"
     CDS             complement(20409..20975)
                     /locus_tag="JQG95_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5888852.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21187..>21274
                     /locus_tag="JQG95_002318"
     CDS             21187..>21274
                     /locus_tag="JQG95_002318"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5888853.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5266 a         5274 c         5466 g         5268 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta caacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcacc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat tgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatccttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcaggggttc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgggtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtcc cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaaaaag aaacagcggc tggtccgctg tttctgcatt cttacggtaa agataaaaat
    15361 aaatagtgac gcgcttcgca accttttcgt tgggtaatta tcaagctggt atgatgagtt
    15421 aatattatgt taacagcgtg tatatcattt ggggttgcga tgacgacgaa cacgcatttt
    15481 agaggtgaag aattgaagaa ggtttggctt aaccgttatc ccgcggacgt tccgacggag
    15541 atcaaccctg accgttatca atctctggta gatatgtttg agcagtcggt cgcgcgctac
    15601 gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga ccttccgcaa gctggaagaa
    15661 cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg ggctgaagaa aggcgatcgc
    15721 gttgcgttga tgatgcctaa cttattgcaa tatccggtgg cgctatttgg cattttgcgt
    15781 gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc cgcgtgagct tgagcatcag
    15841 cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta actttgccca cgctctggaa
    15901 aaagtggttg ataaaaccgc cgttcagcac gtaattctga cccgtatggg cgatcagcta
    15961 tctacggcaa aaggcacggt agtcaatttc gttgttaaat acatcaagcg cttggtgccg
    16021 aaataccatc tgccagatgc catttcattt cgtagcgcac tgcataacgg ctaccggatg
    16081 cagtacgtca aacccgaact ggtgccggaa gatttagcct tcctgcaata caccggcggc
    16141 accacaggtg tggcgaaagg cgcgatgctg actcaccgca atatgctggc gaacctggaa
    16201 caggtcaacg ccacctatgg gccgttgctg catccaggta aagaactggt agtaacggca
    16261 ctgccgctgt atcacatttt tgccctgacc attaactgcc tgctgtttat cgaactgggt
    16321 gggcagaacc tgcttatcac taacccgcgt gatattccag ggttggtaaa agagttagcg
    16381 aaatatccgt ttaccgctat cactggcgtt aacaccttgt tcaatgcgtt gctgaacaat
    16441 aaagagttcc agcagctgga tttctccagt ctgcatcttt ccgcaggcgg tgggatgcct
    16501 gttcagcaag tggtggcaga acgctgggtg aaactgaccg gacagtatct gctggaaggt
    16561 tatggcctga ccgagtgcgc gccgctggtg agcgttaacc cgtatgatat tgattatcat
    16621 agcggtagca tcggtttgcc ggtgccatcg acggaagcca aactggtgga tgacgacgac
    16681 aacgaagtac caccgggtca accgggtgag ctttgtgtca aaggaccgca ggtgatgctg
    16741 ggttactggc agcgtccgga tgctacagat gagatcatca aaaatggctg gttacacacc
    16801 ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca ttgtcgatcg taaaaaagac
    16861 atgattttgg tttccggttt taacgtctat cccaacgaga ttgaagatgt cgtcatgcag
    16921 catcctggcg tacaggaagt cgcggctgtt ggcgtacctt ccggctccag tggtgaagcg
    16981 gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg aagagtcact ggtgactttt
    17041 tgccgccgtc agctcacggg atacaaagta ccgaagctgg tggagtttcg tgatgagtta
    17101 ccgaaatcta acgtcggaaa aattttgcga cgagaattac gtgacgaagc gcgcggcaaa
    17161 gtggacaata aagcctgagc gttaagtcag tcgtcagacg ccggttaatc cggcgttttt
    17221 tttgacgccc actaaagaga aaacaatttg aattaccaaa tgattaccac ggacgatgcg
    17281 ctggcttctt tgtgtgaagc cgtccgtgtc tttccggcga tagccctgga tactgaattt
    17341 gttcgtacgc gcacttatta cccgcagctg gggttgattc aacttttcga tggcgagcat
    17401 ctggcgctaa ttgatcctct cgggatcacc gactggtcac cgctgaaagc gatcctgcgc
    17461 gatccgtcca tcacaaaatt tctccatgca ggcagtgaag atctggacgt gttcctcaat
    17521 gtctttggcg aattaccaca acccttgatt gacacgcaaa tccttgctgc cttctgcgga
    17581 cgcccgatgt catggggttt cgcttccatg gtggaagagt attccggcgt tacgctggac
    17641 aagagtgaat cgcgcaccga ctggctggcc agaccgctga ccgaacgtca gtgtgaatac
    17701 gcagcggcgg atgtctggta tctgttaccg atcaccgcca agcttatggt agaaacggag
    17761 gcctccggct ggctacctgc ggcgctggat gaatgccgcc tgatgcaaat gcgtcgtcag
    17821 gaagtcgttg cgccggaaga tgcctggcgt gatatcacca atgcctggca gttacgcaca
    17881 cgccaactgg cctgtctgca actgttagcc gactggcgac tgcgcaaggc gcgagagcgt
    17941 gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt ggtcggtcgc gcgttatatg
    18001 ccgggaagtt taggcgaact ggacagcctg ggtttatccg gtagcgaaat ccgctttcac
    18061 ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat tgccggaaga tgccttaccg
    18121 cagccgatgc ttaacctgat ggacatgcct ggttatcgta aagcgtttaa agcgattaag
    18181 tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg ccgaattgct ggcatcgcgt
    18241 cggcaaatca accaactgct gaactggcac tggaaactga aaccgcagaa caatttgccg
    18301 gagctgattt ccggctggcg tggtgagctg atggcggaag cattacacaa tttattgcag
    18361 gaatatccgc agtaaaatct tccgaagccg gactgggcgc gctcagcgcc acatccggct
    18421 ttggcaaact acaaatccaa caccaggcgt ttacccttcg cacgcgaaca acagatcaac
    18481 atactttgct ggctggcacg ctcttcatcg ctaaaatact gatcccgatg gtcagcttca
    18541 ccttccagta ttgccgtttc gcaggttccg catacccctt cacgacataa acattccact
    18601 ttcgcggctt tattattttc aataacctgc aaaatagtca tctcttccgg caccacaaac
    18661 tcttttccgg aacgggcaag caccagggta aatgcatcgc cggttttgtc ttcgatagca
    18721 aattgctcaa agtgcagtgt atcggcggcg atgtccagac gcgcagcttc acttcttacc
    18781 gcttcaatta gcgcctcggg gccacaggta taaacgtgtg tgccaggttc gatatccgcc
    18841 aataatcgcg ctaattccag tcgtgttccg gttgatgaat gatgcaaatg gactttctca
    18901 gcctgcggat gctggactag ctcagcacga aatgcgcaac tttctggatt tcgcgagcag
    18961 taatgtagct gccagtcgat gtcgctgtgt tgcaactctg ccatgtgcga caggaaaggg
    19021 gggataccaa taccgcccgc gataaacaga tgctttctgg ctgagggaat tagcgcaaac
    19081 aggttattag gcgttgagat cgttaaccga tcgcccactt ttacctgctg atgcaaaaag
    19141 cgggaaccgc cgcgcgagtt ttcttccagt cgaacggcaa tctgataact agaggtgtta
    19201 tgcggtgaac tcagtagtga atacgcattg ctgtactggt tatcaccatc gctcatctgc
    19261 actaggatgt gacttcctcc ggtgaacgca ggcagcggtt tgccatcggt tgccaccagc
    19321 gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc tcacctgtac ttcaaacatt
    19381 tgatagtctg acattgctgt actccatcaa aaatcagccc agcggcggaa aataccgccg
    19441 ccgatgtcat tagtccttaa acacctgcgc cagcagatta tggaaatggg cgataccatg
    19501 ttcggaaatg ccactaccgc tactgtcggc catgatgcgc ccctgaccac gatagccacg
    19561 cgatttcagc cctttctgta cgctttcaac cagacgtaaa tcttccggat ggaacacatc
    19621 gcgataccac tcaatcagcg atttttgctc gtcggttaac tcttcattgg tgaagtaaat
    19681 atcgtagttt tgcagggtag tttcagaatc caccgggaat tcataaatga ccgtcatcat
    19741 ccctttgatc ggggtgacgt tcagcatcgt gcacggccac agccagaaac cgtggaatgc
    19801 cgcatccgta ccctcttcaa atttaaacga ctgttcggac ggtttggcga aaccgtattg
    19861 cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca acctgtacgg agtcggagaa
    19921 acctagatgc gccggaccac agtgatagca ctcgagatag ttatcgacaa tgttcttcca
    19981 gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc agattgtgga cttccgggca
    20041 ggcttccagc actttcgcgc ccagaccggt gtattgattc acttgaagta cgaaaaaaaa
    20101 ccgggaggac attggattat tcgggttctg atgggattag atttggtggg gcttgcaagc
    20161 ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa agatagtaaa aaattgcttt
    20221 tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc atttcaataa cagcttcaaa
    20281 aaacgttcaa aaccgataac aaccaggctg taaccaaatg actcatatca caaatcagct
    20341 catgccgttt aggtatgtta catgtgtgat tatgtgaggt gaagtatgtt ttagctggtt
    20401 catggttgtt atacggcttt ttttacatct tgtggttctt gtgaaggagc tacaagacct
    20461 tcctgttcat aaataccata ctttgacaaa atctttttac gagtatctgt tggtaatgat
    20521 tcaagcattt tggctgtttg taaggtttgc tgcgaaatac cttgcaaatc agcctggatg
    20581 gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa atcctttctt tctgaacaac
    20641 tcaatcgcag aatgcagttc cttgtcctct aacaattcga caattgcgat cagtttagtt
    20701 ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt gattattttt atgctcaacc
    20761 attggcatgc tctgactttg gataaaccac tctattggac gccctgttgc gtaggctatt
    20821 tgtgctaatc gatctaaaga cgggtatgtc ttgccagaca ggtagtttct gataacccca
    20881 tcagaaagtc cgcacttagc tgcaaacgcc cggcttgatt ctccattcat tgcctcttca
    20941 aggcgttcct tgaaacgcat tatttccggg ggcataaaaa tgcgctctgc atttttgctt
    21001 tctttttcac ctgtcatgcc caatctctct gttatataaa gaattttaca gaattctttg
    21061 atttgcactg gttaaaataa aaatgagcat agctcttgaa ttgagcattt ttctgcggta
    21121 tcgtttttcc cgatagcggt agccggtatc agttaccgaa aacgttaaga gagtaaggat
    21181 cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc agataaaggc tgctgttcgt
    21241 atgaagggga agacactatc tcaattatcc agag
//
LOCUS       DADLCQ010000066        21255 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000066 DADLCQ010000000
VERSION     DADLCQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.066"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..793
                     /locus_tag="JQG95_002319"
     CDS             <1..793
                     /locus_tag="JQG95_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY5888854.1"
                     /translation="VAAQPTIVPQLQQQVTGLTSSLNSQEKKELTHKLESIFNNTQVQ
                     IAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSDRTVRIQVGYGLEEK
                     VTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQYPANPSESESTSSTD
                     HYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDLFLFSDKTFSIAVFS
                     FFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGASGRW"
     gene            complement(853..2151)
                     /gene="eno"
                     /locus_tag="JQG95_002320"
     CDS             complement(853..2151)
                     /gene="eno"
                     /locus_tag="JQG95_002320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY5888855.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="JQG95_002321"
     CDS             complement(2239..3876)
                     /gene="pyrG"
                     /locus_tag="JQG95_002321"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY5888856.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="JQG95_002322"
     CDS             complement(4104..4895)
                     /gene="mazG"
                     /locus_tag="JQG95_002322"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY5888857.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4991..7225)
                     /locus_tag="JQG95_002323"
     CDS             complement(4991..7225)
                     /locus_tag="JQG95_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY5888858.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="JQG95_002324"
     CDS             complement(7273..8574)
                     /gene="rlmD"
                     /locus_tag="JQG95_002324"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY5888859.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8631..11387
                     /gene="barA"
                     /locus_tag="JQG95_002325"
     CDS             8631..11387
                     /gene="barA"
                     /locus_tag="JQG95_002325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY5888860.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLVLESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="JQG95_002326"
     CDS             complement(11619..12959)
                     /gene="gudD"
                     /locus_tag="JQG95_002326"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY5888861.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12980..14320)
                     /locus_tag="JQG95_002327"
     CDS             complement(12980..14320)
                     /locus_tag="JQG95_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY5888862.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14322..15674)
                     /locus_tag="JQG95_002328"
     CDS             complement(14322..15674)
                     /locus_tag="JQG95_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888863.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16108..16557)
                     /locus_tag="JQG95_002329"
     CDS             complement(16108..16557)
                     /locus_tag="JQG95_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY5888864.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16575..17357)
                     /gene="truC"
                     /locus_tag="JQG95_002330"
     CDS             complement(16575..17357)
                     /gene="truC"
                     /locus_tag="JQG95_002330"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY5888865.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRYLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17357..17686)
                     /locus_tag="JQG95_002331"
     CDS             complement(17357..17686)
                     /locus_tag="JQG95_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY5888866.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18308..18853)
                     /gene="syd"
                     /locus_tag="JQG95_002332"
     CDS             complement(18308..18853)
                     /gene="syd"
                     /locus_tag="JQG95_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY5888867.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18921..19769
                     /gene="queF"
                     /locus_tag="JQG95_002333"
     CDS             18921..19769
                     /gene="queF"
                     /locus_tag="JQG95_002333"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY5888868.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19881..21245
                     /gene="ppnN"
                     /locus_tag="JQG95_002334"
     CDS             19881..21245
                     /gene="ppnN"
                     /locus_tag="JQG95_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY5888869.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5225 a         5670 c         5074 g         5286 t
ORIGIN      
        1 tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg gtcttacgag
       61 tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta ttttcaataa
      121 tacccaagtg caaattgctg tattaattgt ttccacaacc aaagatgaga caattgaaca
      181 atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta atgatgggat
      241 actgatcgtt gttgcctggt cggatcgcac tgtccgcatc caggtaggtt atgggctgga
      301 agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga tacccgcatt
      361 taaacaacaa aaattagcta agggattaga gttagctata atcgctttga ataaccaact
      421 cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt cttccactga
      481 tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt tctggttttt
      541 tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt cagcaatcca
      601 tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat tttccttctt
      661 ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga aaagagcatc
      721 tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg gcggcgcatc
      781 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
      841 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
      901 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
      961 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1021 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1081 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1141 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1201 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1261 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1321 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     1381 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     1441 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     1501 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     1561 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     1621 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     1681 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     1741 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     1801 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     1861 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     1921 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     1981 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2041 agcagctgcc atgccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2101 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2161 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2221 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2281 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     2341 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     2401 ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     2461 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     2521 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     2581 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     2641 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     2701 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     2761 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     2821 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     2881 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     2941 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3001 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3061 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3121 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3181 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3241 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3301 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     3361 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     3421 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     3481 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     3541 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     3601 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     3661 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     3721 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     3781 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     3841 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     3901 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     3961 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4021 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4081 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4141 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4201 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4261 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4321 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     4381 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     4441 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     4501 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     4561 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     4621 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     4681 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     4741 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     4801 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     4861 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     4921 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     4981 ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5041 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5101 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5161 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5221 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5281 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5341 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5401 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     5461 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     5521 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5581 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     5641 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5701 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5761 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5821 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     5881 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5941 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6001 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6061 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6121 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6181 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt tttccacccg
     6241 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6301 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6361 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6421 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     6481 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     6541 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6601 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6661 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     6721 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     6781 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6841 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6901 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     6961 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7021 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7081 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7141 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7201 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7261 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7321 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7381 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7441 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7501 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7561 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7621 aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7681 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7741 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7801 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     7861 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7921 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7981 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8041 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8101 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8161 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8221 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8281 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8341 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8401 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     8461 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8521 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8581 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8641 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8701 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8761 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8821 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8881 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8941 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9001 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9061 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9121 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9181 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9241 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9301 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9361 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9421 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9481 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9541 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9601 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9661 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctggtt ctggaaagta
     9721 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9781 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9841 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9901 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9961 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10021 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10081 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10141 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10201 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10261 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10321 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10381 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10441 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat gctgaaaaac
    10501 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    10561 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10621 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10681 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    10741 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10801 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    10861 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10921 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10981 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11041 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11101 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11161 ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11221 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11281 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11341 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11401 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11461 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11521 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11581 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11641 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    11701 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11761 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    11821 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    11881 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11941 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12001 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12061 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12121 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12181 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12241 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12301 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12361 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12421 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    12481 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12541 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    12601 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12661 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12721 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12781 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    12841 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12901 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12961 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13021 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13081 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13141 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13201 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13261 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13321 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    13381 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13441 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    13501 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13561 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    13621 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    13681 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13741 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13801 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13861 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13921 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13981 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14041 cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14101 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14161 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14221 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14281 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    14341 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    14401 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    14461 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14521 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14581 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14641 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14701 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    14761 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    14821 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14881 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    14941 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15001 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15061 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15121 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15181 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15241 accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15301 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15361 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15421 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15481 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15541 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15601 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15661 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    15721 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15781 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15841 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15901 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    15961 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16021 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16081 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16141 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16201 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16261 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16321 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16381 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    16441 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16501 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16561 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16621 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16681 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16741 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16801 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatat
    16861 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16921 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16981 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17041 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17101 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17161 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17221 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17281 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17341 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17401 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17461 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17521 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17581 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17641 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    17701 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17761 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17821 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17881 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17941 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18001 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18061 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18121 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18181 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18241 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18301 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18361 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18421 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18481 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18541 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18601 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18661 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18721 tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac ggccagcttt
    18781 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18841 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18901 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18961 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19021 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19081 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19141 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19201 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19261 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19321 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    19381 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    19441 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19501 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19561 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19621 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    19681 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19741 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19801 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19861 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19921 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19981 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20041 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20101 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20161 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20221 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20281 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    20341 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    20401 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    20461 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    20521 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20581 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20641 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20701 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    20761 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    20821 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20881 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    20941 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21001 agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt gcgttctccg
    21061 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21121 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21181 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21241 cgtaatctcc cctcc
//
LOCUS       DADLCQ010000067        20837 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000067 DADLCQ010000000
VERSION     DADLCQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.067"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JQG95_002335"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="JQG95_002335"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY5888870.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="JQG95_002336"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="JQG95_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY5888871.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="JQG95_002337"
     CDS             complement(2650..3201)
                     /locus_tag="JQG95_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY5888872.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="JQG95_002338"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="JQG95_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY5888873.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5216
                     /gene="cybC"
                     /locus_tag="JQG95_002339"
     CDS             4830..5216
                     /gene="cybC"
                     /locus_tag="JQG95_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY5888874.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="JQG95_002340"
     CDS             complement(5261..5725)
                     /gene="nrdG"
                     /locus_tag="JQG95_002340"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY5888875.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="JQG95_002341"
     CDS             complement(5883..8021)
                     /gene="nrdD"
                     /locus_tag="JQG95_002341"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY5888876.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8415..10070)
                     /gene="treC"
                     /locus_tag="JQG95_002342"
     CDS             complement(8415..10070)
                     /gene="treC"
                     /locus_tag="JQG95_002342"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY5888877.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10120..11541)
                     /gene="treB"
                     /locus_tag="JQG95_002343"
     CDS             complement(10120..11541)
                     /gene="treB"
                     /locus_tag="JQG95_002343"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY5888878.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11660..12607)
                     /gene="treR"
                     /locus_tag="JQG95_002344"
     CDS             complement(11660..12607)
                     /gene="treR"
                     /locus_tag="JQG95_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY5888879.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12792..12845
                     /gene="mgtL"
                     /locus_tag="JQG95_002345"
     CDS             12792..12845
                     /gene="mgtL"
                     /locus_tag="JQG95_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY5888880.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12986..15682
                     /gene="mgtA"
                     /locus_tag="JQG95_002346"
     CDS             12986..15682
                     /gene="mgtA"
                     /locus_tag="JQG95_002346"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY5888881.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="JQG95_002347"
     CDS             complement(15888..16274)
                     /gene="ridA"
                     /locus_tag="JQG95_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY5888882.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="JQG95_002348"
     CDS             complement(16347..16808)
                     /gene="pyrI"
                     /locus_tag="JQG95_002348"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY5888883.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="JQG95_002349"
     CDS             complement(16821..17756)
                     /gene="pyrB"
                     /locus_tag="JQG95_002349"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY5888884.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="JQG95_002350"
     CDS             complement(17760..17894)
                     /gene="pyrL"
                     /locus_tag="JQG95_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY5888885.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18175..18570)
                     /locus_tag="JQG95_002351"
     CDS             complement(18175..18570)
                     /locus_tag="JQG95_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY5888886.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="JQG95_002352"
     CDS             complement(18702..19415)
                     /gene="bdcA"
                     /locus_tag="JQG95_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5888887.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19486..20079
                     /locus_tag="JQG95_002353"
     CDS             19486..20079
                     /locus_tag="JQG95_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5888888.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIAKRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20224..20676
                     /locus_tag="JQG95_002354"
     CDS             20224..20676
                     /locus_tag="JQG95_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY5888889.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4963 a         5226 c         5519 g         5129 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgccgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa gaagcgcagg
     5161 ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat cgttaattcc
     5221 tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa tgatgcacca
     5281 cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc acaaatttgc
     5341 cgtcgaccag cacgttaatc aaatcaacaa cctgcatttg cgcagcgttg agttcgtcga
     5401 gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg atgcgtttta
     5461 ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg gagagggaaa
     5521 tcccctggcg tttgatacga gtgtcattca gatctttaat gatcttgtct tccattgctt
     5581 tggtaaatgg ctgaccagaa tttacccgcc atgtgctttt gttatagcaa ccggggcatt
     5641 catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg acgatgtcga
     5701 caggatagta ctgatgataa ttcattattt cgcctggctt atcagtccgc gtgtaggctg
     5761 catccggtaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta cagccgttgc
     5821 caaatgtagg ccggataagg cgtttacgcc gcatccggca tttgcttagc gcagagtgaa
     5881 gattaaccta tctgcccatt ccccaaatgt ttaacgcggc gcttaacttc ttcctgctta
     5941 ccagcgttaa acggacgtgc atccgggcta cctaaatatc cgcacacgcg gcgagttacc
     6001 gacacacggg aggcgtcatg gttaccacat ttcgggcaag tgaagccttt gctggtgcac
     6061 tcgaactcac cggtaaagcc acactcgtag cactcatcaa tcggtgtatt ggtgccgtaa
     6121 tacggtacat gctgatagct gtaatcccag acatcttcca gcgctttcag gttgtgctga
     6181 atgtttggat actcgccgta gcaaatgaaa ccaccgttcg ccagcggcgg gtaaggcgct
     6241 tcaaagtcga tcttgtcgta cgggttcacc ttcttctcca catcaaggtg gaaactgttg
     6301 gtgtagtaac ctttatcggt cacgcccggc accacgccaa actcagcagt atcgagacgg
     6361 cagaagcggt cgcacaggtt ttcactcggc gtgctgtaaa gactgaaacc ataacccgtt
     6421 tcttctttcc actgatccac tgcctgacgc agacgttcaa caatggcgat acctttcgcg
     6481 cgaagcttct cgttgtcgta aacatgctcg ccgctgaaca gcgcgttaat ggtttcgtgg
     6541 atgccgatgt aacccagcga aatagacgca cgaccgtttt tgaagatttc agaaacatca
     6601 tcgtcagcat tcagacgtac gccacaagca ccttccatat agaggatcgg ggcgacgcgc
     6661 gctttcacgc cttcgagacg agcgatacgg gtcatcagtg ccttacgtgc cagcaccaga
     6721 cgttcatcca gcagcttcca gaaggtggct tcatcgcctt ttgcttccag agcaatacgc
     6781 ggcaggttca ggctgatcac gccgaggttg ttacgaccat cgtggatctg ctcgccgttt
     6841 tcattttccc acacgccgag gaagctgcgg cagcccatcg gggttttaaa cgaaccggtg
     6901 acgttcacta cctgatcgta gttcaggata tccggataca tgcgcttgct tgcgcactcc
     6961 agcgccagct gtttgatgtc gtagttcgga tcgccttttt tatggttcag gccatcgcga
     7021 atcgcaaaca ccagtttcgg gaacaccgca gttttacggt ttttaccgag gcctgcgata
     7081 cggttacgca ggatcgattc ctgaatcagg cgcgattccc agctggtgcc cagaccaaaa
     7141 ccaaaggtta caaacggcgt ctgaccgttg gcggtgtgca gggtgtttac ttcgtactcc
     7201 agcgactgga aggcatcgta gcactctttg atggtacgag agttagcgta gccttcggcg
     7261 tccgggatgt tccactcttc tgcggtcttg cgatgtttgt tgtagctggc ggtaacaaac
     7321 ggcgccagca cttcatcgat acggttaatg gtggtgccgc cataaatatg gctggcgacc
     7381 tgagcaataa tctgcgcagt taccgcggtt gccgtagaga tcgacttcgg cggttcaatc
     7441 tcggcgttcc ccattttaaa accctgggtc agcatgcctt tcaggtcgat caacatgcag
     7501 ttgaacatcg ggaagaacgg cgagtaatcg agatcgtgat agtgaatatc gccacgctca
     7561 tgtgcctgca ccacgtcacg cggcagcagg tgctggcgtg catagtgttt agccacgatc
     7621 ccggccagca ggtcgcgctg ggttggaatc accttgctgt ctttgttggc gttttcgttg
     7681 agtaacgagg cgttggtctg ctcgaccaga ccacggatct cctggttcag gcgaccgcgt
     7741 ttttcacgtt caatgtcgcg atcgtgacgg tactcgatgt aagcacgagc cagttgtttg
     7801 tatggacccg acatcagctg gttttcaact gcggtctgga tctcgttgat atccacctgg
     7861 ttgcgcccct gcatctgctc gttgacaacc gcggcaacag tggcgcaata atcggcatca
     7921 tcgacttccg ctgctttagc tgcacgcaga atcgcttctt tgatgcgctc tgatttaaac
     7981 ggcactttgc agccgtctcg tttcatcaca tgcggtgtca tgatcgctcc atatttttaa
     8041 gaacaggtta tccacagaaa ttgggaaagg cgttttccgg ttttttcgtt tcgttttccg
     8101 gtgctttccg caatcgccat ccgcttatcc acaacgccgc acctttttcg tggggagctg
     8161 tagtagcaat tatagtcgat taatacaaca tattgggttg ggatgcattt taaagtctat
     8221 atatagtgct ttgcatcaag gatgttttaa ctttttttga tgtagctcaa agtaaaaagc
     8281 agagcgtacg gatgacgggc gctacagcgt tatgtaaatt ttttaatgaa tttgctggtt
     8341 gaaaaatcaa caaaaacaac atgctgacag acaaaacccc ggaatgaccg gcattatcgg
     8401 ggcttaggga agatttactt ctgtaaccac cagacagcct caaaaggccg taaattcatg
     8461 gcacagggtt gtggtgaggc ttcttcgtaa ttatgcatca caagctgcca gttgccgcgc
     8521 atttgccctg gctgccaggg ttggatctca cggctaaggt tggcaatgac cagcaaggtt
     8581 tgccccttcc attcacggcg atagcaccac aatacagggc tgtttggcag cagatcctgg
     8641 taattgcccc atgtcaggat ggcttcttgc ttacgcagtg cgattaactt ttggtaggtg
     8701 taaaacacag aggagtcatc ggccagcgcg gcttctacgt tgatttgttg atagttatcg
     8761 cctaggccaa tccacggttc gccagccgta aacccggcat tatcgccgtt gctccattgc
     8821 atgggcgtgc ggctgttgtc acgggattta ctggcgagga ttgccaataa ctcgtcggca
     8881 tcacgaccat cgttgcgcag ctcggcaaac atattgaggc tctccacgtc gcgatagtca
     8941 gtaatgcgcg tgaaatgcgg gttggtcatg ccaatctctt cgccctggta gatatacggc
     9001 gttccctgca tgccatgcag caccatcgcc agcatttttg ccgcaggcac gcggtattca
     9061 ccttcatcac caaagcgaga aacaatgcgc ggctgatcgt ggttacacca gaacaaggca
     9121 ttccatgcca cgttgtgcat tccttgttgc cagtggcgga acaatgtttt caacgccaca
     9181 aagtcaggtt tagccagcgt ccatttttca ccaccgggat aatcgacctt caggtgatga
     9241 aaattaaagg tcatcgacaa ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca
     9301 aggctggtgg aggacatttc acctacggtc attaacccgc gtggcgtaaa cacatcgcgg
     9361 ttcatctcgt gcaaaaactc gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg
     9421 ccatccaggt cttcagggaa acgcgggtct ttggagatca gattcaccac atccaggcgc
     9481 aacccgtcga ccccacgatc ggcccagaat tcacagactt ttttcagctc tgcgcgtacc
     9541 gctggattct cccagttgag atccgcctgt tctggtgcaa agagatgcaa atagtactgt
     9601 tcgctttccg catgccagcg ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt
     9661 ggcgtttctg gttcgccatc gcgccagata taaaactggc ggtaagggct ttctttgttc
     9721 agcgcctcgc gaaaccaggc atgttgggta gaggtatggt taaacaccat atcgagaatg
     9781 atacgaatcc cgcgcgattt tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg
     9841 taggtgggat caatcgccgt atagttcgct acatcgtaac cgttatcgac ctggggagag
     9901 acataaaagg gggttagcca gatggcatca acgcccagtt tatgcagata gtccaggcgt
     9961 tggataacgc cacgtaaatc gccggtgccg ctacccgtgg tgtcctgaaa actctttgga
    10021 taaatctggt agataacgcc gttttgccac cagtggggaa gattagtcat tacgttattc
    10081 ctgcaaatgc gagggggcgc aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc
    10141 caggcggtat ttccgctgat agataaacga ggtgagtaca atcgggatga tgatggcgat
    10201 agccattgcc agcgcaaata cctgccagta gctcggttga atagagagaa ttcccggcag
    10261 gccccctacg ccgataccat tcgccataac gccgttcagg ccgcatagca atcctgccag
    10321 accagaacca atcatcgcgc acagcatcgg gaagcgatat ttcaggttga tgccgtacat
    10381 tgcaggctca gtgaccccaa gccaggcgga gatagcggca ggcacggaga tctcgcgttc
    10441 attgtgcttg cggctggaaa tgataatgcc tatcacggcg gagccctgag cgatattcga
    10501 cagcgcaatc agcggccaca ctggcgtgcc acccatgctt tgaatcatct gcaaatcaat
    10561 agcaagggtg gtctggtgta cgccggtgat caccagcggc gcgtacagga agccaaacaa
    10621 tgccgcacca atcggagcaa agctgccggt catcaggtga cgtaccgcaa aggcaacgcc
    10681 atcgccaatc atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat
    10741 cagcgaacag acgggcacca ccaccagata gaggtaatcc ggtacgatgc gtttaaggcg
    10801 agtttcaata acgcccagcg ccagcccggc taacagtgcc gggatcacct gcgcctgata
    10861 gcccactttg gcgatgctga acatgccaaa gtcccacact tccggcagct gctgcccgag
    10921 cagataagcg ttcatcagct ggggggaaac cagtgtcacg ccaagcacga taccaaggat
    10981 cggcgtgccg cccatttttt tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa
    11041 aatcgcttca ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag ggtacatttg
    11101 cgccagcgtc tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa aaccgaggat
    11161 caaaccgccg ctaatcaacg cgggcagcaa cgggaagaag atctccgcga aatgagagat
    11221 caactgctca tgccatttca tattttgccg ggcggctttt tttacctgct ctttatcaac
    11281 ctgcgcctgt ccggttgacg ctattagtgc ttgatagtaa tcacccacgt tggtgccaat
    11341 caccacctga aattgcccgg cattggtgaa acagcctttc accatgggga gttgctcaat
    11401 ttctttcggt ctggcattgg ccggttggtt gaggacaaag cgcaggcgag taatacagtg
    11461 gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca atcaaccgat cgatatccgt
    11521 ttggtttatt ttgctcatca taaagcccca tggcagatga catttttggt tggctgcaga
    11581 atatattgcg cggaaaaatt taaaaacggg aacgttcccg aaacgcagcg aagatcacaa
    11641 tttatcgttc aggaaacgat caggacaggg tggcggggat gatgatttgt tgcggttcgc
    11701 tgcgcccggt tacctgtgcg atcaattggc aggccgcctg gcgtccagct tcggcgtagc
    11761 caggatctac ggtgacgatc tccggatgaa ggaatttcat taacggcgta ttacctacgc
    11821 tcgccagttg caaggtgtcg atgcgttgct cttgcaggta tttacttgcg ccaagagcca
    11881 gcgtgtcggt tgcgcacagt aacgcggtag tttcaggcgt aatcactttt gctacgtttt
    11941 catagccttg cttcatagca agccctggca gagcggcaac gggatgcagt ttatgcgctt
    12001 tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt cgtcacgtca ctatgcggca
    12061 cgccgagata actgatattg cgatgcccct ggtcatagag ccgttgcatc agaatcttga
    12121 ttgccccttc gtcgtcataa cagaccgaag caaagccttt tgcgtcacgc gccagcagaa
    12181 ccagcgatga ctgccagtgg gctaacattt cttcggttat gccggtaaaa ccgaacagca
    12241 ctacgccgtc gatattacgc cgtttcagca cccccaaatg ttcggcaact aattgtgggg
    12301 aaaactgatt ttccatcatg attggatcgt aaccttgttc atagaacgct ggcagcatgg
    12361 tttgaacggc gagattttct gacaacgaat ccagacgggt aacaatgatg gcgaccactt
    12421 tatcgctttg cccacgcata gcgcgcgcag agcgggaagg ggaaaatcca tgctgattca
    12481 tcactgcttc aacacgctcg cgcgtgcgct gactcacgcc gctttcgtta ttcagcaccc
    12541 gggaaaccgt agatttcccc acgccgctta agcgcgcgat gtctttgatg gtcagccgat
    12601 tttgcatcct gttgtcccat aacgtgttgt ttaattattt gagcctaact ttacccgtgc
    12661 attcagcaat gggtaaagtc tggtttatcg ttggtttagt tgccagcagg tattatatcg
    12721 ccataaatgc tacgaatatt attggattct ccttattatt tgcggcgctt ttttcactta
    12781 ccggaggtta tatggaacct gatcccacgc ctctccctcg acggagatta aaacttttcc
    12841 ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt ttaatgtccc
    12901 tgctcagctt tattaccttc aggtaaggct tcgccacgcc tgaagacatt tctgtactgt
    12961 ttcagacagt gcggagggac tccttatgtt taaagaaatt tttacccggt tcattcgcca
    13021 tttaccttcc cgtctggttc atcgcgatcc attgcctggc gcgcagcaga cagtgaatac
    13081 ggcggtcccg ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc ccgaagaaga
    13141 attgtggaaa acgttcgaca cccatccgga aggattaaat caggcggaag tggaatctgc
    13201 ccgcgaacaa catggtgata ataaattacc cgcccaacaa ccgtcgccgt ggtgggtaca
    13261 tttatgggtc tgctatcgca acccctttaa tattttgctc accattcttg gtgctatttc
    13321 ttacgccacg gaagatttat ttgccgcagg tgttatcgcg ctaatggtcg ctatttctac
    13381 gttgctgaac tttattcagg aagctcgttc caccaaagcg gcagatgccc tgaaagcgat
    13441 ggtcagtaat accgcgacgg tactgcgagt aattaacgac aaaggcgaaa atggctggct
    13501 ggagatcccg atagaccagc tggtgcccgg cgatattata aaactggcgg cgggggatat
    13561 gatcccggca gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc aggcgtcgtt
    13621 aaccggtgag tctctgcccg tggaaaaagc cgctactact cgccagccgg agcacagcaa
    13681 tccgctggag tgcgacaccc tgtgttttat gggcaccacc gtggtgagcg gcacggcaca
    13741 agcaatggtg attgctacag gagccaacac ctggtttggt caactggcgg ggcgtgttag
    13801 tgagcaggaa agcgagccga atgcctttca gcaagggatc agccgcgtta gtatgctgct
    13861 gattcgcttt atgctggtga tggctccggt ggtgttgctt atcaacggtt acaccaaagg
    13921 cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg gtaggcttaa cgccggaaat
    13981 gttgccgatg attgttacct cgacgctggc gcgcggcgca gtaaagctgt cgaaacagaa
    14041 agtgatcgtc aaacatctgg atgccattca gaactttggc gcaatggata ttctgtgcac
    14101 tgataaaacc ggcaccctga cgcaggacaa aattgtgctg gagaatcata ccgatatctc
    14161 cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg aacagtcatt accagaccgg
    14221 acttaaaaat ctgcttgata ccgcggtgct cgaaggtacg gatgaagagt cagcgcgttc
    14281 gctggccagc cgttggcaaa aaattgatga gattccgttt gatttcgagc gtcgtcggat
    14341 gtcggtggta gtggcagaaa ataccgaaca ccatcagctg gtttgcaaag gtgcattgca
    14401 ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtac cgctcgatga
    14461 caccatgctg cgtaagatta agcgggttac tgatacgctg aatcgtcagg ggctgcgcgt
    14521 ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga gattaccagc gggcggatga
    14581 atccgacctg atcctcgaag gatatattgc tttccttgat ccgccaaaag agacaaccgc
    14641 tccggcactg aaggcattaa aagcgagtgg aattaccgta aaaatcctta ctggcgacag
    14701 tgagttagtt gcagcgaaag tgtgccatga agtggggctg gatgcgggag aggtggtcat
    14761 tggtagtgat attgaaacgc tatctgacga tgaactggca aatctcgcac agcgcaccac
    14821 gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt gtgactttac tgaagcgcga
    14881 agggcatgtg gttggcttta tgggcgatgg tattaatgat gcgcccgcct tacgcgctgc
    14941 ggatatcggc atttctgtgg acggcgcggt agatattgcc cgtgaagcgg ctgatatcat
    15001 cctgctggaa aaaagcctga tggtgctgga agagggggtt attgagggac gtcgcacttt
    15061 cgccaacatg ctgaaataca tcaaattgac ggcgagttct aacttcggta atgtgttcag
    15121 cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg ttgccgttac acttgcttat
    15181 tcagaacctg ctgtacgatg tgtcacaggt ggcgatcccg tttgataacg tcgacgacga
    15241 gcaaattcaa aagccgcagc gttggaatcc ggcggatctg gggcgcttta tggtcttctt
    15301 cggaccgatc agctcgattt tcgatattct gacgttttgc ttgatgtggt gggtattcca
    15361 tgccaacacg ccggaaacgc aaacgctgtt ccagtcgggc tggtttgtgg tgggattact
    15421 gtcgcaaacg ctgattgtgc atatgatccg tacccgccgt gtgccgttta ttcagagctg
    15481 tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg attgtcggga tcgcattgcc
    15541 gttttcaccg ctggccagtt atctgcagct gcaggcgctg ccgttaagct atttcccgtg
    15601 gctggttgcg attctggcag ggtatatgac gttaacccag ttggtgaaag ggttctatag
    15661 ccgtcgttac ggctggcagt aaagaataaa ctgggcatgg tggcccggat tcagattgca
    15721 gacaaagtga gctttgttca tgccggatgc ggcgtaaacg cccaatccgg cctacaaatt
    15781 caataaattg caggaatcac gtaggcctga taagcgtagc gcatcgggcg attttgcttt
    15841 tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta gcgacgaaca
    15901 gcgattgctt cgatctcaat cttcacgtct ttcggcagac gggcaacttc aacgcaagaa
    15961 cgtgccggga aagtggcgtt gtgttcggtg aagaaggctt cgtaagtggc gtttacggtt
    16021 gcgaagtcgt tcagatcttt cacgaacaca gtggttttaa cgatgtcgcc cactttcagg
    16081 ccagcggctt cgacgattgc ttttacgtta tccagcgact gacgtgcctg tgcagcgacg
    16141 tctgccggta cttcaccagt tttcggattt accgggatct gaccggaggt gatgatcata
    16201 ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt ttccgtcgcg
    16261 atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct cattataggg
    16321 agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg ggaaaactct
    16381 ttttcacagt atttgcattt aagcgcgatg tcattggcgc gttttcgcac ggcaaagctg
    16441 gatgaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag cacattgtcg
    16501 atgcgctctg gcagacttgg gcgcgattta cccaccactt catagttgtc gatacggtta
    16561 accgtggctt gcggtgcata caatgccagt tggtctactt gctcttcact caaaaaggta
    16621 ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaaatt cagaccaatg
    16681 gtgatgcgct gatctgtttc ggtcaacttg aacagactca acagcttaaa accgatctgg
    16741 gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa tttattatcg
    16801 tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag tgccagtaac
    16861 gcctggcgag cgaaaatccc gttgcctgct tgctggaagt accaggcgtg aggcgtttta
    16921 tccacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt catattggct
    16981 ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt ggcgtactcg
    17041 gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac ttccgccatc
    17101 acttcttcaa tagagctgtg cagactccag gcgatccctt tttcatcgag catatccaga
    17161 atgtattgcg gcattgccag cgcatccggc gcgataaagt aaaaacggtt gccgtcgaac
    17221 ttcgctagcg cctgggtcag ggagtgaacg gtgcggccat atttcaggtc accaaccatt
    17281 gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tggtgaataa gtccagcaag
    17341 gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac attgccagaa
    17401 aattcggtgg ctaggcgcgc cgcaccttcc tgcggatgac gcatcactat cgcatcgacg
    17461 taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cttttttacc cagtgatgta
    17521 ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcatgga agtttcgaaa
    17581 gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt gtgcttcaac
    17641 agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag attaaggtca
    17701 tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt agccatcttt
    17761 tatctcctga cacctaggca aaaaaaagcc cctcgattga ggggctggga atgggtgatc
    17821 aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac aaaatgtcga
    17881 acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat agccagaagc
    17941 ggtgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa ttaaaagtca
    18001 aaatatatgc atttttattt tctatgattc tggaggggaa gtgtcgttcg gtatcaataa
    18061 ttgcagacat ttcctgcttt tttctttttc attttcacgc aatcagaaat gcattttatt
    18121 ccattcatcc gacttatagg gcggagggtg aaaacgaacg gctaacacta ttgcttatag
    18181 ctcagggata cgcgctatca ctttaatttc aaaatcaaag cctgccagcc aggtaacacc
    18241 caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca ccgtcatgat
    18301 gtcttcaaat tgtttttccg gatcggtatg aaagctcgta acatcaatga tatcgtcaaa
    18361 agtgcatccc gcagctgcca gggtcgcatg caaattatca aaggccagtc tgacttgttg
    18421 ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg aaacaaacag
    18481 caaatcgccg gaacgaatag ccgcagaata acgatgctca gcatacagtg aatgtcggcc
    18541 agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta cgacatgaac
    18601 agaagattta tatttatcgg gagccgcttt tagggggacg acgttagtaa acaaaaccca
    18661 gacatcatgg ataatggctg ggcttaattg agcgtagtcg gttatgcgcc aaacgcgcca
    18721 tcaatggtat gcatcgcgcc ggtaacaaaa ctggcttctg gccctgctaa ccatgcgacc
    18781 ataccagcga cctcttccgg ttgcccatgt cttttgatgg ccataaaact atgcaacata
    18841 tcgcgcattg gcccgttggc gggattaacg tcggtatcaa ttggtcctgg ctggacgacg
    18901 ttgatggtga tccctcgcgg tccaaaatca cgggccagcc cgcgcgccat gccttgcagg
    18961 gcagatttac tggcggcata agcagccatg cctgcaacag gcatacgatc gccattcacg
    19021 gagccgatga ttaagatgcg cccgccttcg ggcatctgcc gggcggcttc aacagaggca
    19081 tgataaggag catgaatatt gattttgaaa aggcgatcaa tatcgtcggc atttaattcc
    19141 agggcatcgc caaagacgcc aatacctgca tttaccacca ggatatccaa tgcgccgctc
    19201 ttacgaacga catcaataac agcgtctctg tcagcactat ctgtgaatac tgctgtcgct
    19261 ccagtctctt gtgccagacg ttcagcggca tctttcgacc ccgcataggt gaatcgtaca
    19321 ttggccccat cggtgacgaa acgacgtacg atagcggcac cgattccacg actgccaccg
    19381 aggatgagaa ctgtcttacc ggtaaaagcg cccataagga ctccttgatt tattatgtaa
    19441 catgcattac aaaactgttt taactttctg tcaacaggtt ttgtaatggt cactaaaaaa
    19501 caatctcgcg ttccaggtcg tcccagacgt ttcgctcctg agcaggcagt ctctgcggca
    19561 aaagtgcttt ttcaccaaaa aggtttcgat gcagtaagtg tctctgaagt tactgattat
    19621 cttggtatta accccccgag tctctacgcg gcttttggca gtaaggctgg gttatttagc
    19681 cgtgtactca atgaatatgt cggtacggaa gctattccgc ttgccgatat tcttcgtgat
    19741 gatcgtccgg taggcgagtg cctggttgag gtattaaaag aagcggcgcg cagatatagc
    19801 caaaacggcg gctgcgctgg ctgtatggtt cttgaaggta ttcatagtca tgatccacaa
    19861 gcgcgtgata ttgccattca atattatcac gccgcagaaa cgaccattta tgactatatc
    19921 gccaagcggc atccacaaag cgcacaatgt gtgactgatt ttatgagtac cgtgatgtca
    19981 gggctttctg cgaaggcacg ggaggggcac tccatcgaac aactctgtgc aacagctgcc
    20041 ctggcagggg aagcgataaa aactcttctc aaggagtgat gctggccttg atccgaaagg
    20101 cgggaacgcg cctgccgata agttgtgata agacaataat tcacgcgtta aggctggcgg
    20161 aattgatcat cttttcgtat aacgatagaa atgaaacatt attttaatta aggagtggaa
    20221 aagatgatca tcggaaatat tcataatctt cagccgtggc taccccagga gttacgccag
    20281 gcgattgagc atatcaaagc acacgttacg gcagaaacgc caaagggcaa gcatgatatc
    20341 gaaggcaatc gtctgtttta tcttatctcg gaagatatga ccgagccgta cgaagctcgc
    20401 cgtgcggagt accatgcccg ctatcttgac attcagattg tgttaaaagg tcaggaaggc
    20461 atgaccttca gcacgcaacc tgcaggcgcg ccggataccg actggttagc tgataaagac
    20521 atcgcatttt tgccggaagg cgttgatgag aaaacagtta tcctaaatga aggtgatttt
    20581 gttgtgtttt atccggggga agtgcataaa ccgctgtgcg cagtgggcgc gccagcccag
    20641 gttcgcaaag cagtagtgaa gatgctgatg gcgtgatgac ttttcgccgt aaataactcc
    20701 aggtttacgg cgagtttgtg aaaagagcgt tttttgatat ttttttgtga gtaaaatttg
    20761 taatgcttag acgttcttat taactcaagg agttcgtcat gggcaaaata tcagatttga
    20821 attattctca acacatt
//
LOCUS       DADLCQ010000068        20745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000068 DADLCQ010000000
VERSION     DADLCQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.068"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            20..550
                     /locus_tag="JQG95_002355"
     CDS             20..550
                     /locus_tag="JQG95_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5888890.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRAKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEVLAKVIPFFNTHLG"
     gene            568..1206
                     /locus_tag="JQG95_002356"
     CDS             568..1206
                     /locus_tag="JQG95_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5888891.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1497..2225
                     /gene="mpaA"
                     /locus_tag="JQG95_002357"
     CDS             1497..2225
                     /gene="mpaA"
                     /locus_tag="JQG95_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY5888892.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2200..3159)
                     /gene="ycjG"
                     /locus_tag="JQG95_002358"
     CDS             complement(2200..3159)
                     /gene="ycjG"
                     /locus_tag="JQG95_002358"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY5888893.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3278..3784
                     /gene="tpx"
                     /locus_tag="JQG95_002359"
     CDS             3278..3784
                     /gene="tpx"
                     /locus_tag="JQG95_002359"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY5888894.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3828..5369)
                     /gene="tyrR"
                     /locus_tag="JQG95_002360"
     CDS             complement(3828..5369)
                     /gene="tyrR"
                     /locus_tag="JQG95_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY5888895.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5517..6578)
                     /locus_tag="JQG95_002361"
     CDS             complement(5517..6578)
                     /locus_tag="JQG95_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY5888896.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISHSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6575..7972)
                     /locus_tag="JQG95_002362"
     CDS             complement(6575..7972)
                     /locus_tag="JQG95_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY5888897.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8128..9126
                     /locus_tag="JQG95_002363"
     CDS             8128..9126
                     /locus_tag="JQG95_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY5888898.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADKSFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9237..10109)
                     /gene="ompG"
                     /locus_tag="JQG95_002364"
                     /pseudo
     CDS             complement(9237..10109)
                     /gene="ompG"
                     /locus_tag="JQG95_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10182..11263)
                     /locus_tag="JQG95_002365"
                     /pseudo
     CDS             complement(10182..11263)
                     /locus_tag="JQG95_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11277..11936)
                     /gene="pgmB"
                     /locus_tag="JQG95_002366"
     CDS             complement(11277..11936)
                     /gene="pgmB"
                     /locus_tag="JQG95_002366"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY5888899.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11933..14200)
                     /gene="ycjT"
                     /locus_tag="JQG95_002367"
     CDS             complement(11933..14200)
                     /gene="ycjT"
                     /locus_tag="JQG95_002367"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY5888900.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14197..15251)
                     /locus_tag="JQG95_002368"
                     /pseudo
     CDS             complement(14197..15251)
                     /locus_tag="JQG95_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(15261..16036)
                     /locus_tag="JQG95_002369"
                     /pseudo
     CDS             complement(15261..16036)
                     /locus_tag="JQG95_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16054..17106)
                     /locus_tag="JQG95_002370"
     CDS             complement(16054..17106)
                     /locus_tag="JQG95_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY5888901.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17137..17979)
                     /locus_tag="JQG95_002371"
     CDS             complement(17137..17979)
                     /locus_tag="JQG95_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY5888902.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(17966..18847)
                     /locus_tag="JQG95_002372"
     CDS             complement(17966..18847)
                     /locus_tag="JQG95_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY5888903.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18868..20151)
                     /locus_tag="JQG95_002373"
                     /pseudo
     CDS             complement(18868..20151)
                     /locus_tag="JQG95_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20165..>20745)
                     /locus_tag="JQG95_002374"
     CDS             complement(20165..>20745)
                     /locus_tag="JQG95_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY5888904.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5061 a         5654 c         5045 g         4985 t
ORIGIN      
        1 aaaacgaatt aaagctgtag tgagtattac gggtgttaat atcgggcgtc tttttcggga
       61 aggtttcagc aattatgatc cgattggcgt attgaatgca atggcgtcac aacgagcaaa
      121 agaggctcgt ggtggagaat tacagatcaa cgaattactt cccgccagcc tggatgcagc
      181 aaaagcccat ggactgaccg aacgcgatgt ttatgaagca actgactact ataaaacacc
      241 acgcgggcaa caaccgggag gtgcgacaaa aatgttgttc tcacatgcac agaaaacgct
      301 ggcctgggat gccttcgcat ttactgaagt gttactgacc cagccggtga tggtagtagt
      361 tggtgaaaaa gtcggtgcct tcggtgctta tcgtgatgga ctggaagtct atggccgggc
      421 gatggtttcg caggatcgtc agttagtttc gttacccgat ttctcacatt atgaactata
      481 tgacaaaccg gaagcagttc aggaggtgct ggcaaaagtt attccttttt tcaacaccca
      541 tctgggataa gtttcatgag aaatattatg aaaaatgtac tgattcttgg tgctggcggt
      601 cagatagccc gccatgtgat taatcaactg gcagataaac aaactattaa acaaacttta
      661 tttgcccgtc agccagcaaa aatacacaaa ccctatccaa ctaatagcca gattattatg
      721 ggggatgtac tgaatcatgc agcactgaag caggccatgc agggacagga tattgtgtat
      781 gccaacctta cgggggaaga tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa
      841 gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg gtatttatga tgaagttcca
      901 ggaaaatttg tcgaatggaa taatgcagtt attggtgaac ccctaaaacc attccgcagg
      961 gctgcggatg caattgaggc ttctggactg gaatatacaa ttcttcgccc tgcctggctg
     1021 actgatgaag atattattga ttatgagctt acctctcgca atgaaccatt caaaggtacg
     1081 atcgtatcac gtaagagtgt cgcagcatta attacagata tcatcgataa acctgagaaa
     1141 cacattggtg agaatatagg catcaatcaa cccgggactg atggcgataa gccattcttc
     1201 atgtaaatca attatataga taccatcgta ctgtactatg tgcagtacga tttattaaac
     1261 ttattaaggt gaactgatca atcagatgcg attaacatgt gtagaaccag taagaataaa
     1321 atacgccatg tatataacat tacgacaatt ctaatatctg cacatcaatg aaaatgcaaa
     1381 caacttccgg caaaaaagtg atacaagatc gatgacatag cgttgaagaa aaggatttga
     1441 taatccgcaa aatttgcaca acactgcctg taatacttcc tgaaccagag acgatcatga
     1501 ccgtaacccg ctcacgcgcc gaacgcggcg catttccgcc cggaacagaa cattacggac
     1561 gttcattatt gggcgcgccg ttaatctggt ttcctgcccc tgcagccagc cgcgaaagtg
     1621 gtttgattct ggctggcact cacggtgatg aaaactcttc ggtcgtcacg ctctcctgtg
     1681 ctctgcggac tttgacacct tctttgcgcc gccatcatgt ggtgctgtgt gtgaatcctg
     1741 acggctgcca gctggggtta cgggccaatg ctaatggtgt ggatttaaac agaaactttc
     1801 cggcggcgaa ctggaaggaa ggtgaaacgg tttatcgctg gaacagcgcc gctgaagaac
     1861 gtgatgtcgt attactgaca ggtgacaaac ctggctccga acctgaaacc caggcgctgt
     1921 gccagcttat acaccgcatt caacctgcct gggtggtctc attccacgat ccactggctt
     1981 gtattgaaga tcccagacac agcgaattag gcgaatggct ggcccagtcg tttgaattgc
     2041 ctctggtaac cagcgtcggt tatgaaacgc ctggctcttt cggcagttgg tgtgcagatc
     2101 tcaacttgca ttgcatcacc gcagagtttc cgcccatctc ctccgacgaa gccagcgaaa
     2161 aatatctctt tgcgatggca aacttgctgc gctggcatcc taaagatgca attcgcccgt
     2221 cgtgaactga agcgccggtt ccacatctac cgccagccag gtcggtccgt caagatcggc
     2281 gaaactgacc tgcggcacca gcggtaaagc ggcgctaatg gcacgagagg tacacaacat
     2341 gcagcccagc atcagactga aaccttgtgc acgcgcttca gtcgccagcg cttccgtcag
     2401 accaccggtt ttatcgagct taatgttaac catctcatag cgccctttca gcgccttcag
     2461 attgctacga gtatgacaac tttcatcagc acaaatcggc aacggatgaa taaaattctc
     2521 cagcgccgca tcgtcctgcg caggaagcgg ttgttcaagc atcgcaacgc ctaaatccgc
     2581 caatagctgg caacgcgccg ccaacccttc tgcacgccag gattcatttg catcaacgat
     2641 cagcgtggca tcgggcacag cggtgcgaat tgccaccatc cgctcactga taagatggtt
     2701 atccagcttc actttcagta atttcgcgcc tgcctgccag agtgttgatg cactattggc
     2761 catctgatca ggcgtaccga tgacaaccgt ctgtgcagtg ataactgtct cgggaagcgt
     2821 tatgccgatc aaatcagcca gcgattgctg ctgttttcgc gccgcgagat cccacaatgc
     2881 acaatccagc gcattacgtg ctgcgccggc agggagaatt ttttgcaact cctcccgtgt
     2941 cagccctttc tctaattgcg gcacgacgct cataatttgc gccattaccg aggcatcact
     3001 ttctccataa cgcggatacg gcgtgcattc gccggtgcct ttaataccct cttcttccag
     3061 ttcaaccacc accacgcgcg cttcactgcg acttccccgg gcaatcacaa acggggtatg
     3121 taagggccag gcttcctcaa ataccttaac gattctcatc tttactcctt gttgtcgcgg
     3181 tggtctgcgg attaagcgca aatagcgttt gctgtgttat tgacagttag cataaactag
     3241 gtgtgacgtt aactatatgt aaacaggaaa gataatcatg tcacaaaccg ttcatttcca
     3301 gggcaacccg gttacagtcg ccaattccat cccgcaggcg ggtagcaaag cgcagacttt
     3361 tactctcgtg gcaaaagatc tgtctgacgt caccctcggt cagtttgcgg gtaaacgcaa
     3421 agtgctgaac attttcccga gtattgatac cggtgtttgc gccgcatcag tacgtaagtt
     3481 taaccaactg gcaactgaga tcgacaacac cgttgtgctg tgtatctctg ccgatctgcc
     3541 gttcgcccag tctcgtttct gcggcgcaga aggtctgaac aacgttatca ccctctccac
     3601 tttccgtaac gctgaatttc tgcaagccta cggtgtggca attgctgatg gcccactgaa
     3661 aggtctggca gcgcgtgccg ttgtggttat tgacgaaaat gacaatgtga ttttcagcca
     3721 gctggtggat gaaatcacca ccgagccgga ttacgaagca gctctggctg tactgaaagc
     3781 ataagttaat atgcctgatg gtgttgcacc atcaggcata ttcgcgctta ctcttcgttc
     3841 ttcttctgac tcagaccata ttcccgcaac ttattggcaa tcgcggtatg tgaaacgccg
     3901 agacgttttg ccagtttgcg cgtgctggga taattgcgat aaagctgggt taataccgag
     3961 cgttcaaaac ggctggtgat ttcgtccagc gaaccttcca tcgcatcttc gcccacggct
     4021 accgttgcgg cgtcataatc cggcaacaaa atatcctgtg gacgcagctc ataaccgtcc
     4081 agttgtgtca gtgcgcgata gatagcgttc tttaactgcc gcacatttcc cggccacgca
     4141 taacgtgtaa gtacagtatt caggtcagcg gccagtttcg gacgcggcac gccctgctcg
     4201 tcggcaaagc gggcgacgaa cagctcagtt aacggcataa tgtcctgcgg acagtcacgt
     4261 agcggcggca gattgagcgt caacacgttc agacgataat agagatcttc acggaacacg
     4321 cctttttgca ccagttcgac cagattcttc tgcgtagcgc aaatcacccg cacatcgaca
     4381 tgcacctcat ggtcttcgcc aacccgacgg aaagtgccat cattaagaaa acgcagtaat
     4441 ttcgcctgca tccgtggtga catttcccct atttcatcca acagcaccga accaccgttc
     4501 gcctgctcaa agaacccttt cttcccttcc ggagcatgac caaacagttc actctcgacc
     4561 gcatcttctg gtatagacgc gcaattcagc gccaggtaag gtttgcccgc tctggggctt
     4621 gcctgatggc aggcgtaggc aaaaagatct ttacctgtac ctgtgtcacc cgtaatcagc
     4681 agcggcgcgc ttagcatcgc cagtttctgc gcctgttcga caacgtgctt cattttcggg
     4741 ctgacggcga caatttggct gaaggcgctg acgtcctggg cggcgacatt ttgcaactgg
     4801 cggcccatac gaatcgttga tcgcaacatc accaccgcac cggtcaggac gtgttgatca
     4861 ttttcatcct gaagataaac aggcgtaatc tccatcagga aattctgccc attaataacg
     4921 acatgctcgt tatgcgaatc ttgcggttcg ctttccagcc aacgtaaaaa attaaagccg
     4981 ttaatcaatt gtgcggcggt atggttgcgc aggcgatcca atttttgccc aaaaagctga
     5041 cagctcgccg ggttcgccat atccactttg cttttcatat cgacagagag cacaggttca
     5101 ggcaacgcct ccagtaacgc gctcaacgcc agatgctcac gttcggaagg catccacggg
     5161 acagtacgca catcggtaac acctgcaata cggcgtattt cggccatcag actgctgaaa
     5221 ctctcaaact ccagttcagc aaaattgagg tagattcgcc caatgggatc aatctcaata
     5281 ccgcgtaaat caatgcctct tagcacgagt agatcgagta attcgcgggt cagaccgagt
     5341 cggtcttcac aaaagacttc cagacgcatg ggaaccttca cctgaaaaaa gaacaattaa
     5401 tatgatgata tgacactatt tggatagcag gaaggtttct gtcaacaatc attgacacaa
     5461 agacggaaaa acgggcactt cggcgtaaag atatccggtc agcacccgat attgcattat
     5521 ttttcgctgg gcgttttgcc tttttgcaga gtttctttca cctgaccgat aagctgacga
     5581 cggaaatccc caaggcgagg tttgtcatcg tcaatccacg gcagcgggcg gcaaagctcc
     5641 atagctttaa tcccgagtcg taccgtcaga agtccagcac caattccctg agctgcgcgg
     5701 gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc gcgcaccagt
     5761 tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg caaacggctg
     5821 taatacccca gttcaatgcc atacagcgtg gcaatgcgat taattaaacg cagattgcgc
     5881 caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat caacgttgat
     5941 tccgccgccg aatggctgat ttcgcgccgc gcctgggcat ctaaaactgg ctggaccaaa
     6001 tgcgcataca ggctgaccac ttcacgatcg ttctgcgttt catggattga ggcataccag
     6061 cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt ttcgcaaaat
     6121 gcgcgacctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc gcgttcatgg
     6181 gcgcgctgtc gcaagcgcca taagcgccgc cactctgtta ccacagaacc tacgccagcg
     6241 ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg ggtttgccag
     6301 gcattcattg tccactgtat accctgcccg acaacgcttg cgccaaacag agccagcccg
     6361 cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcgtc cattaccgct
     6421 tcgacttgcc cctcttcttc ctgcgcttcg tcgagcgtgg ccggggcaaa attttgcgcc
     6481 tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgttcgac ctccagcgga
     6541 ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct cctattaaaa
     6601 attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc atcacctgcg
     6661 ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg ggcaaacgcg
     6721 caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccgg ttaccgcgca
     6781 atgccggaat tttctcgccg ttgacgtcaa taatgccgct ggtggtcgcc tgcaccgagg
     6841 ccagccccag gcaatccata ctaatccttt caaacgccgc attttgccag gcatcctgaa
     6901 tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca tggtccgctt
     6961 tcatcgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga aacagcgtgc
     7021 gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata tcattaaatg
     7081 cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg cggtcaaagc
     7141 gcaaaaaatg attcttatag aaccccttca ccactttctc gcagtaataa ttaaaccgct
     7201 cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac tcgtcccagg
     7261 catcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata tctcccggca
     7321 agacaaagcg cccaggctga ataaagtgca gcccctgctg tttacagtgg tggagataat
     7381 cggtccacac ggcggcaatg tctgccagcc ggttttcgtc ggcaggcgct agcgggtcaa
     7441 gtccttcgca catcattcgc catttcgccg accattcgcc gcgctgacca ttgagtaagc
     7501 ccgtcatctg gcgcgaccag cttaagtaat cctgcgccag catcggcagg tcgagcaacc
     7561 attcgccggg ataatccaca atttccagat acagcgtgga ggtatcctta aagtggcgca
     7621 gcagcgaatc gttcgattta aaacgtaacg ccaggcgaat ttcactgacg ccgcgcgtag
     7681 gcgtcggcca ggcgggagga tcaccatata actgcgccag tccttcatca taggtgaagc
     7741 gcggaatgcc aaagtcacgc tggggaatgc gttttacgcc cagcaagcgc tcttcacgca
     7801 ccgcgcttaa cagcggcaaa cgtgctccgg catgaatatt gagcaactga ttaaccatcg
     7861 cggtgataaa cgctgttttg ccgctgcggc taagtccggt tacagcgagg cgcagatgtc
     7921 tgtcgacacc ccgattcacc agcgcattga gttcattttt aagtcgcttc attgctgtcc
     7981 tgtgctgctc tgaagaccag aaaaaaaatc attcgtaagg atatgaatgt gggggccagg
     8041 ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag atgtgcagca
     8101 ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat tgccagggtt
     8161 gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa tatctccccg
     8221 gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc aaacaagctg
     8281 gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac ccgttcgacc
     8341 aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac cgccaaagcg
     8401 gaagaagaag gtttcgacgt gatattacaa acgtcgcaca atccggcaga agatctgcaa
     8461 aaatgcgaaa gcaaaattaa acagaaaatg attaaaggca ttattatgct cagttcgcca
     8521 gcggataagt cattttttgc ccaactcgat aaatacgata ttccggtggt ggtaattggc
     8581 aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt tggtgacagc
     8641 attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg cctgcatgct
     8701 ccgcttgatg tccatgtttc ggttgatcgc gttaatggct ataagcaaag tctgtccgcg
     8761 cataatattg cagtgcgtga taaatggatt gttgacggcg gttataccca tgaaacagca
     8821 ctgcaagcag cacggcaatt attaagtcag tcaccgttgc ctgaggcagt gtttgccact
     8881 gacagcctga aattaatgag catttatcgt gcggcggcag agaaaaatat tgctattccg
     8941 cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatttt aacgcctgca
     9001 ccaggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga gttattattc
     9061 cgcttaattt cgggaaaacc gtcaccacag aatattaccg ttgccacgca tatgacgctg
     9121 aaataaatac cagctacctt gcctgaatgc cggatgcgat gctggcgcat cttatccggc
     9181 ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc ccattatcag
     9241 aacgagtaat ttacgccgac acctgcataa tggaatttat cgctgtcgcc ttcgtcgtga
     9301 tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa atcataaccg
     9361 taaaataaac ccacacggtt aaaatcatgg ccttcacgtt caatatcatc ctgccagtcc
     9421 cagttactct agcgatccag cccaatgcgc gtatacggcg tcaccgagtg gttgccgagc
     9481 gtcagcggca aataggcgcg aatttcttgt gtggaaaact caccgttatt gcggctgtcg
     9541 tccatattga agccgcgctc gagataatag ttcactcgca aagcaaccgt ttcgttgaag
     9601 gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt cgtgttcaaa
     9661 tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcatc agtcagtttc
     9721 acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt gcccggttca
     9781 tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact gaaatcatcg
     9841 ctttcgagga actgataatg cacctccagc tccgggcgat caaacctcgt tccacgttta
     9901 cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct ccacggcccg
     9961 ttggcggcat taaaatagac tgaaggctcc gccagcccat ccatatcttc gccataaccc
    10021 tcgacgtttt ctatttcgta catcgcaccg atattaaagt gccagtcgct cttttcctcg
    10081 gcctgtgcgc aggccattcc cgcacacatc accagtgcgg tacagggtaa taactttttt
    10141 tgttccttgt attatcctgg gggaatgcct tccccctgta tttagcgaat tgctatttcc
    10201 gtttctgcat caaagaaatg acatttcgtc atatcaaaat gaatagtgat attttctcct
    10261 gcatgataat catttaacgc accagcacgg accactaact cgtgccccca accgtggtgt
    10321 agagcataaa ttccgcaccg gttaattctg ccacgctaat tttggcggaa atgttatttt
    10381 cctcttgcgc gtccggatga atatcttccg ggcgtattcc catcacgatg ggcttatgca
    10441 aactttcctg tgttttcaga accgctaatt tctcttcgag aatggttaat ttaagcgttt
    10501 ccgtaacgaa tttatcgcca tcgatcgtgc cgcgaataaa attcatcgct ggtgatccaa
    10561 taaatccggc aacaaacata ttcgcgggtt ggttataaac ggttttcggc gcacctactt
    10621 gctgaacaat cccgtctttc ataatcacaa tccgcgccgc catggtcatc gcttcggtct
    10681 gatcgtgggt cacgtagatc atggtggtgt tcagtttctg atgcagcttg ctgatctctg
    10741 cacgcatttg tacgcgcagc ttggcatcaa ggttagagag cggttcatcc attaaaaaca
    10801 cgcccgcttc gcgcacaatc gcccgcccga gcgccacacg ctggcgttgc ccgccggaaa
    10861 gcgcgcccgg cttgcgtttc aggtactcac gcaggccgag aatttgcgcc gcccagttca
    10921 cccgctcatc aatcacctct ttggcgattt tttgcatctt caggccaaac gccatgttgt
    10981 cgtaaaccgt catatgcgga tacaacgcgt agttctggaa caccatcgcg atattgcgtg
    11041 ctttggctgg aacgtcattc attcgtttgc cgtcgatcaa cagatcgccg ccgctgatct
    11101 cctcaagccc ggcaatcatg cgcagggtgg tcgatttacc gcagcccgac gggccgacaa
    11161 atacgatgaa ctctttatcg gcaatttcca ggttgaagtc cttcaccaca tgcacctggt
    11221 tatcgtagat tttttgaata tgttgtaacg aaagctgagc catgttgatt cctttgctat
    11281 acgttttgcc agaaggccga taaccgcgac caggtcaggg agtccgttga gggcaacagt
    11341 aattgcgccc cggttaagcc cgcaccgatc cccaccgagc gcataccgct ggcgttaatg
    11401 gcgtcaatgc ccgcctgcgc atcttcaatg ccgatacatg cctgcggcgg cacgcccagc
    11461 ccggcacagg cagcgagaaa gatttccgga tccggtttcg agtttttaag ttgggaagca
    11521 tccgcgcaga aggtgaaaaa ctcgcgcagc tccagcgccg ctaaaatcgt cggcgcattc
    11581 agggagacgg aagccagccc taccggaatc tgctgtgcac ggagctctgc cagcaaattg
    11641 cgaataccgg gcagcacagc attaaccgtc agctcgcgca gtgagtggac gtaaagcaga
    11701 tttttgcgat acgccagttg cgcccgctcc tgcgggttaa agtcgccctc tttgcccccg
    11761 tgttgcagaa tgcgccgcag agactcatcg cggctgatcc cttttagaga ttcgttaaac
    11821 tgcgcatcaa tactgatgcc aatttcagcg gcaatctgct gccacgcctg gaaatgcaga
    11881 tgcgcggtat cggtgattac accatccaga tcgaaaatta ccccttgcag tttcattcat
    11941 cctcctgatg cgtggtagcg gtcccattga agggcaaaat aaaatcaccc aaacagaaaa
    12001 cagattcttc agccacggat ataagctgac cgttcaaacg cagtgaaacg ggcgcagaag
    12061 ttcgaatcgc aatacgctgc gcgtcgagag tgacctgtaa ttcgcagccc tgccagaaca
    12121 gagggaaaga caactgttgc cactgctcaa gtaacgccgg atttagatgc aattcaccgt
    12181 cacgcacgct caccccggca aaaccctgaa tcgcccccag ccagatagcg ccagttgcgg
    12241 cagcatggat accatcatca caactatgcg gatcagcacc aagatcgatt tcagtcccct
    12301 cgcgccagaa ctgataactt tgggtcagca ggccacagcg tgcagcaaca atgccgtgga
    12361 ttgctttact taatgacgag tcgtgaatag tgcgcggttc ataaaattgc agattggcaa
    12421 gacacgatgc cgatgagaac tgctctggca gcatgtaatt gagcatcacc acatcagctt
    12481 gtttgaggat ctgcatctcg ttcacttctg cgcgtgaata atccagcaga atggtttgct
    12541 tccccgccgc cgctttgtat ttcgccagat taatcgccgg cttagtcata aacgaatcat
    12601 cctgcggcaa aacaccgtcg ggctgaattt ctggcagccg cagttcttta aggaacattt
    12661 cggcacgatg aataaacgcg tcgtcactac agccaaactg gcgggcaata ctcagcgcct
    12721 gctggacgtt gtaatacacc atatagctgg tgaaggcgtt attattgaca tgttcggtat
    12781 attcgtctgg cccaataaca tcatgaattt ccagacggtc gttaaccctc accgcgcggc
    12841 taatccagaa ctttgcagtt tccagaagta gcgccatgcc ttcatgcgca atgaaacttt
    12901 catcccccgt ggtctgccag tattgaataa ccgcccaggc gatatcggcc accagatgat
    12961 gttccgcctg cgccgaggcc actttttgcc gcagcccggt gcgaatgtta atggcggcaa
    13021 attccggcgt ctcttcttcg ccgctgcgcg cgctttccca cggaaatagc gcgccctgcc
    13081 agccgttgcg tcgcgctttc tcctgcgcgc ctggcaagtt gtgccagcga taacgcagta
    13141 aacttcgggc aaccgtcgga tcgctaaaca gatggaacgg cagcaaaaat acttctgtat
    13201 cccagaaaac gtggcctttg tagccttcgc cggttaagcc ttttgccgca atactgctgc
    13261 gctcgccgtg agccggcgtc atgatgcgca gatgataaag cgcataatct aacgcttgct
    13321 gatcgtgcgc atcgccgcca tttaccgtga tacgacgttt ctgccaccat tgacgccagt
    13381 tttctgttga tgctgcaaga tgttggtcgt aactctgttg cgcgcacatt tcaagctggc
    13441 gaagcgacac gctgccccac tcgtctaaag cagcttgcct gtcatcccgc cagtcgatcc
    13501 acaccagttt ttgcaacgtc actgtctcgc ctgcatgaag ctgcgtactg gtatgttgca
    13561 gtaaacggcg ctctttggcg gtatagcatt gctgcacatc accgctcacc tggcagcaac
    13621 aactgatggc cacatcactg cgcccatcct gggtggtgta gatcccctgc atcaaatgct
    13681 gaccaaacac ccgcacctgg gtttcgtcga gatgctggcg accgtggttg gtttgcgtgg
    13741 cgtcaatgcc tgttgaaatc agcactgacg cgtcggcgtc cagtggcata atagtgattt
    13801 ccagcgcaat gagcggcagt tggtctgccg aaacaaagcg acggctggcg atggtgtaac
    13861 ctgcgccgtt gctggtacgc cagacaacgc tacggcgtaa ttctccactg gcaaagtcaa
    13921 gttcacgctg ccaggcttcg cgggataacg agaaaacctc accatttatg gcaatctcca
    13981 tccccaagat atcaggcaga ttcaccagtt cgttgatttc accttttccc gcccgatgat
    14041 acagccccgc cagatacatc cctcgcgtct ggcgcgtgta atcttcttca tggctggcgc
    14101 gaagcccaag atagccgttc ccctgcgcca tcagcgatgc atacttgttc agggtatgct
    14161 ggctgaaatg gggttctgat aacgttactg gcctggtcat aattccacac gcgtccctgt
    14221 ttctgcggat tgatataacg ccgccaccag ttgctggatg atgtacccct gctcggcgtc
    14281 ggctatcatc acgggcttgc cctgtacgtg attgataaag gcttccatgc tgcgcaaatg
    14341 gcggttgtcg tctgccattt cccgttgcat cagcgtcatt aattcaccgt tgttatcggt
    14401 gtagatatgt gctggaaaca gcgtcgcacc ggctttatca ccacaaaagc tgacgttcat
    14461 aatcaactgt tcgcggatgt tgagtgcaaa tgacgtttcc agccacagaa tgccgccgtt
    14521 atgaaattca atggtgccaa acagcgaatc ttcgacgctg taagttgccg gatcccactc
    14581 gccaaattga ccacagctct tttgcgtgcc gatcttttga aaactatgcg cattcacgct
    14641 tttcaccgcc ggaaagccca gcacatacat cgcagcatcc agcatatgaa tgccgatgtc
    14701 gatcagcagg ccaccaccct gcagttcttt attggtaaag acaccccagc cgggaacgcc
    14761 gcagcgacgc agggcgcggg cggtggtaac gtaaatctct cccaaaacgc cgttggtcac
    14821 ctgttcacgc agctgttgcg tatcgagcgc aaacgatggt gaaagtcgta ggccagcacc
    14881 ttgcccagtt tgcgcgcggt atcgcacatt tcccacgcct gttctgtcgt catggcgggc
    14941 ggtttttcgc acatcacatg gcagcctgct tccagtgcca tcagggtatg ttcgtaatga
    15001 aaacggttag gtgagccgac gctaaccaca tcaggtttca ccgccagcag catggcctgc
    15061 ggatcgtccc acacggatgc attcccgtat ttttctgcca gcgcctgcgc ctgggaaagg
    15121 cggctgtcac agacagccac cagttccaga tcgttgcggg tgcagtagta cgaagcatga
    15181 accttatccg ccacctggcc cgcgcctatt atcgcgaccc gcagcggaga gcttgtcatt
    15241 gcacttttca caaagacctc ttagcaggta cgcaaccagg ccaacgaatc acggtacgcc
    15301 tgggcaggat cttctgcccg gatacgccct tcatacacca cataaccctg atagttatcg
    15361 gcgcgcagct gttcaaacag cgcgtggaaa tcgagggtgc cactgcccgg ctggtaacga
    15421 tggttatcgg caatatgcac atgaccgagc aagtcacggt tgtcgtgcag cgcctgcgcc
    15481 aggttatctt cttcgatgtt catgtgatag aaatcgccga taatctgcac atgtttaaga
    15541 ttgttttcga cgatgtaacg acgagcatcg gcgagggtgt tgatcatatg atcctgatag
    15601 cggtttaacg gttcgagata caccacggtt ccggtacgcg cggcgacctg ttccagtacg
    15661 cgcagggaat cactcaccag tttgcggtcg ccgtccaggc tacgcggcga ggtcatcggc
    15721 ggtaagcgga aggtaaacat gccccacgct gccggaaccc ccccacttct gccagcgcct
    15781 cgagaatgcg ttcgatctgc tttaagccat taagacgacg ctcttcgata aagtcaccaa
    15841 tccagccgtc atagccgccg caggcggtgg tcactggtaa accggtttct ttgatcgccg
    15901 ctttgacttc ttcgatgttg ttgaccagca gtttgccgtc aatctcaaaa ccatcgaagc
    15961 ccatctcttt gatataacga aatctctcgc ggatattttc cggaaagaat gcctgattct
    16021 gtgtgccgat tttcattttt tcattccttt gaattaaaac gtaacgccca ttttgatgct
    16081 ctgttccgga tgctgatcga catactgcat gtagctttcc gggctgttgg caaaggtcac
    16141 taccgggtcg attaaatctt cgcaattgag ataaccgttc atcagcagtt cccagcaggt
    16201 ttcttcaata cgcttacggc tccagcgcgg ataatccggg ttcggttcgc tgcacgcgcg
    16261 ggagaaaaca attttggcgt tattgaaatg cgcttcgcgt ccgaggttaa aaccttcagc
    16321 aaatggtttg gcaaacgcga cataggagat ggtgccgccg taggccaggc cgcgcagcgc
    16381 cgattgcagc gcgtcggcgt aaccgctggt ttcgataatc acatcagccc cctgcttgcc
    16441 ggtcagcgtt ttgatctctt tgcctacgtc agtgccaatg gggttaaggc agaaatcagc
    16501 accgtggcga cgggcaatat cacagcgatg ggcgatagga tcgacgccaa ttaccaccga
    16561 tgcgccagcg cgtttcgcca gttggatggc aatttgaccg atcgcgccaa gccctaccac
    16621 cacgacaaaa tcccctacgc gtacgttggc atcgcgcacg ccgctcatgg caaactgcgc
    16681 cgggtcgtaa cagacggcgt ttttccagga gctgccttgc ggcattttgc gcagcttgta
    16741 gttattcact gcgttaatga tgaccgtctc ggagagcggg ccgtagccgc atacgctgtc
    16801 gcccaccgcg tagtctgtaa cgtcgctgcc gcactcgata atgtcgccaa ccaccatgtt
    16861 gccaagctgg aatttgccaa actcaatgcc gcgcggcgca tctgccggac gcggggtgaa
    16921 catctgccac tcgccgttaa agtcttcatc gataaacggg ctggcggcgc ggaagtcgac
    16981 cacttccgtt ccgtgtttcg gtgcgccgaa acgcacgcgg atcttcactt cattggctaa
    17041 aatcgcccga tcttcatact caaccagcgc agcaacacgc ggtgctgtgg ctactaactt
    17101 tttcatgtaa aactccttgt aaagaaactg gccgctttag cccttcacgc caccggcggt
    17161 caaaccactc ttaataaaac gttccgaaag cgcatacata atcaccaccg gcaatgcggt
    17221 caccagcgag gccgccatca tccgtcccca gatgtaatca ggcgtactga acagcgcgtt
    17281 caggcccact ggtaaggtga aattgctggc gctggagagg aaaatcgacg caaacagata
    17341 gtcgttccac gccaccataa agcagtagac gaacaccgat atcagcccgg acatcgccag
    17401 gggcacggtg atacggaaga tgatttgcag acggttgagg ccatccatca tcgccgcttc
    17461 ttcgatctca tcagggatgg tgtcgaagta acttttcagc ataaacacgg cggtgggcag
    17521 ggtttgcgtc accatggtaa tgatcagcgc catctcggtg tcgtaaatac caagcgcggt
    17581 gatgattttg aacagaggca ccaccagcaa aatgccagag aacatataca ccgtgtaaaa
    17641 gctggcgttg atggtcatcc ggcctttaaa gcgcaggcgg gaaagcgcgt aagctccgag
    17701 aatgccgaga aataccgcca ccacggagga aaccaccgac accaccatgc tgttacggaa
    17761 gtagtcgaca aacgggaaaa tcatcgggtt aaaaatgtcg acgtaatgct ccagcgtcca
    17821 ctgttgcggc agcagcgtag gatgcagcga tatcgcctct ttcgcgcttt tgaacgaggt
    17881 catcagcatc acaaaaaacg gaaacagcgt gatgatgaga aacagcgcca gcccgcagta
    17941 aaaaccgata cgactgagcg tgcgtttatt tgttgccatt gaggttcacc cgtttcctgg
    18001 tcagcagaat gacagcgaaa ataatgatga agagcaccac cgagatcgcc gccgctttcc
    18061 ctaagtcatt aaaggcaaag gcggttttgt agagatagac accaagaata tcgaccttgg
    18121 ttgtcagcag ataaacatca gcgaacatgt agaacatcca gatggtgcgc agggtcacca
    18181 ccgtcgccag gaccggcata attgcgggca gcgtgacgat gcgaaaccgc tgccaggcat
    18241 tagcgccatc catctctgcc gcttcgtaca gcgatttgtc gatggtctgc aaaatcgcca
    18301 gaaacgagat aaaggcatac gggaagtagc gccagatggc gaacagcacc accagcacaa
    18361 agctactgcc cggattatca aaccacagcg gtgcctgctc atagagatgc agaagatcga
    18421 cgccgaggta gttcacaatg ccgtagccgt tgttgaacat gtatttccag gcaaacacca
    18481 gcgaaatgga cggcgttacg taggagagga tcaccagcga acgcgcggtt ttgcgcaggc
    18541 ggaactcgcg gttgaaaaac atcgccacgg ccagcccgag cacggtgctg cccgccacca
    18601 ccagcgccgt ataccagacc gtcatccaca gcgagtgcca gaagccggga tcggagagaa
    18661 tgcgcacata gttactcacc ccaacaaacg ttgactcgat gttgggattg agcggcagac
    18721 gtaaaaaact gatttcgata ttcgacacca tcggccacgc caccagaccg cccagcagta
    18781 ataagctggg cgcgagaagc agcagcgcaa acggcatatc ggaacgacct gaaaaaagcc
    18841 tgttcattgc ggattcctgt cctgggttta gtgctgttcg atcagttcat ccagcttttt
    18901 ctggctcgct tgcagcgtgc cagggagatc ggctttaccc acggtgacgt tatgcaccat
    18961 ggagctcacc acaccagaac ccgtcacatc gcccatgcgg gtaaagtttt tatcccctac
    19021 tgcgccaaag acctgaatat ttggcagttc accgattagc tgattcggta gttcacccag
    19081 cgccttaata acgtcgttgt ctttccaggt ggcggtagtg acaaccgctt tattcaccgg
    19141 cagcgcagca cctggcgaca tcatcaccca gtcggcaatg ttgtccgcct gctccataaa
    19201 ggtgacaaat ttttctgctg cttcggtctt ttgtccggcg gtaatggtca gcgaggtcaa
    19261 catgccgtag accgcagagt ttttctcggt tggcaccacg aaaccgacgt ttttcgggtc
    19321 gccttcttta atcacagccg gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc
    19381 gttcataaag gcgtctttca cttccatgat gtcgttagaa cccggcatgg tgttggcagc
    19441 aaggtcgcgg taataggtca gcgcctgcat catctctggt gtatcaaggg tgattttgcc
    19501 ttcggcgtta aagacgttgg cctagttgga taacgcaaac tgggagaagg attgttccgt
    19561 cagcacgctt tctgccgtag gcagcgcaat accgtatttt ttattcgccg ggtcattcag
    19621 tttctgtgca acgtccagca gctgttgcca gtttttcggc tcctccagcc ccgcttttgc
    19681 cagcacatct ttgcgatacc agatgtcgcc aatccaggcg ctgacaggaa caccggtcca
    19741 tgcgctacca tcttcggtac gcacaatacg cagtacgcca tcgtaaaacg cgccttcacc
    19801 aacgttgctg atgaccgtgg caaccgcttt gcgatcgata agctgctctt tatccataac
    19861 tttggcgtag tcatggctgg tttcgatcac ttccggcagc gagccgctac gtgaaagggt
    19921 gatgactttg gtgttatgag cgtcttcttc cacgggcact tgtttaacgg tgatacctgg
    19981 gttttctttt tcaaaacggg caatcaattt gctgataact gcctgacgct cctgctccac
    20041 cgaagaatgc ataaattcga tggatacatg ggttttattt tcttctttac agcctgaaat
    20101 cagggcgcag gaaaccagtg ctgataacag cacgattttt gatttaatca tgagatgttc
    20161 ctttttattt aatccacata acctgccagg ggcgcagcgt tatttcatta cccaatatat
    20221 caacttcact aatcagatcc cgaccatgca gatcagtaat atttacatgc tgaatatttt
    20281 tactgacatt aaacaatcca gtcagacaat taccatcagc gttacttctt tgaatacgca
    20341 ttacggatga attaaccgta tcgatggtaa aattattatc cggatgaaac tcgttatggc
    20401 tgcgacgaag tgtaattaaa cgcgacaact catgatatac cgcatgcctt aatgtagctt
    20461 catcgttcag ttctcgtgtt atctcttcac tgtgatattt tttacggtta atcgcacggt
    20521 tatatccgag tttttcgaca cctgcgtaat cattacgcga gccaagaata ctttgaatat
    20581 atatcgcggg tacaccgggg aaacttaaca aaatcgcatg ggcaaggata aacctggtgc
    20641 aacgttcttc atcgctactc tcgcggcggc ttaacgcatt catataggtg acattcatct
    20701 catagggact gcgcgtgccg tcgggattat ttttccagtt tacta
//
LOCUS       DADLCQ010000069        20724 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000069 DADLCQ010000000
VERSION     DADLCQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.069"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            81..350
                     /locus_tag="JQG95_002375"
     CDS             81..350
                     /locus_tag="JQG95_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY5888905.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="JQG95_002376"
     CDS             344..709
                     /locus_tag="JQG95_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY5888906.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQG95_002377"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="JQG95_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY5888907.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="JQG95_002378"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="JQG95_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY5888908.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="JQG95_002379"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="JQG95_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY5888909.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="JQG95_002380"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="JQG95_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY5888910.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQG95_002381"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="JQG95_002381"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY5888911.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQG95_002382"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="JQG95_002382"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY5888912.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLNAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12404
                     /gene="malT"
                     /locus_tag="JQG95_002383"
                     /pseudo
     CDS             9674..12404
                     /gene="malT"
                     /locus_tag="JQG95_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="JQG95_002384"
     CDS             complement(12447..13463)
                     /gene="rtcA"
                     /locus_tag="JQG95_002384"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY5888913.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13467..14693)
                     /locus_tag="JQG95_002385"
     CDS             complement(13467..14693)
                     /locus_tag="JQG95_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY5888914.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14882..16480
                     /locus_tag="JQG95_002386"
     CDS             14882..16480
                     /locus_tag="JQG95_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY5888915.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16462..17220)
                     /locus_tag="JQG95_002387"
     CDS             complement(16462..17220)
                     /locus_tag="JQG95_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY5888916.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="JQG95_002388"
     CDS             complement(17237..18067)
                     /gene="glpG"
                     /locus_tag="JQG95_002388"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY5888917.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="JQG95_002389"
     CDS             complement(18112..18438)
                     /gene="glpE"
                     /locus_tag="JQG95_002389"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY5888918.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18628..20133
                     /gene="glpD"
                     /locus_tag="JQG95_002390"
     CDS             18628..20133
                     /gene="glpD"
                     /locus_tag="JQG95_002390"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY5888919.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20402..20632)
                     /locus_tag="JQG95_002391"
                     /pseudo
     CDS             complement(20402..20632)
                     /locus_tag="JQG95_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4893 a         5698 c         5231 g         4902 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtt cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    10741 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    10801 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    10861 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    10921 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    10981 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    11041 atgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat caggatgaag
    11101 cggaacggct ggcaaaactg gcactggaag agctgccgcc gggctggttc tatagccgca
    11161 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    11221 tggcgctagt gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    11281 tatggagttt aattcagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    11341 gggaaacgca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccaa
    11401 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    11461 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    11521 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    11581 cccgtagcca gttgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    11641 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    11701 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    11761 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    11821 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    11881 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    11941 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    12001 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactt aatacgctgc
    12061 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    12121 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    12181 cagaactaat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    12241 actccggtta cagcaatgaa caaattgcgg gcgaactgga agtggcggca accaccatca
    12301 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gctgccgtgc
    12361 agcacgcgca gaaactgctg aagatgatgg gttatggggt gtgagtttag ccggataatg
    12421 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctgtt
    12481 tctatcaaac caaaccgcac cggcaagaaa cgctccacca ccgcgatatt ggtcagcaga
    12541 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggcagtacc
    12601 agttggtcgg cgagatattc ccccaccgcc gctgggcttg ccaggtagcg tttcacctct
    12661 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    12721 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    12781 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaaa tccccgccag tgtagcgatt
    12841 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    12901 atgttcccgc gctcgccaag ttgcaaggtg ctaaacgatg ccacgggcga gacttccgtt
    12961 gccaccacac cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    13021 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    13081 gacgggttat cggtgccgcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    13141 gcgggcagca ccgtttgcag caccagcgta caacttccgg cgctaccgat agcaaagcgg
    13201 taatcgccgc cgcgcacggt gccaggccgg aagaccagac gctgcgatcc cagctccgcg
    13261 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    13321 agcagccccg gtttcgcccg tccggctcga atgccagtga tggtaaatgg ctggccggtt
    13381 atcatcgaca ggctcagcgc cgagcgcagg atctgcccgc cgccttcgcc ctgtgcacca
    13441 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggtg
    13501 tagataactt ccaccagatc gctttgtgcc gccatcaccg catcaatatc tttatacgcc
    13561 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    13621 atttggtctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    13681 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    13741 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcaccttta
    13801 cgcgtcacgt agatctcttc accaaagagc tgttcttttt gcacatagtt gtggtgacag
    13861 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    13921 aacgccgtta ccacattttc catcatcgca tcgcggttaa ggctggcgaa aagctgcgcc
    13981 caggccacgg ctcttaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    14041 tcacgcgacg gcaacgtctc aagttggtcc tgcatctctt tttgcgccag atcgataaag
    14101 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgc
    14161 tccgactcat caaggcagat ttcaataaag tggttacccg ttcccagcgt tcccaggtgt
    14221 ttatagttgt tagtgttaag gaaacgcgga tatttttgcg ttaaccactg ataaccggcc
    14281 tccagctcag cccatttcgc atcgacgtta aaaggcgggt tttcccatgc gcctttatca
    14341 cgtttacaac gcccggtagt acgcccgtgt ggtacggccg tttcaatcgc ttgacgcagc
    14401 tccgccaggt tttcaggcag gtctgccgcc gttaacgcgg tacgcagcgc gttcattcca
    14461 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    14521 ccaatggtgg aacctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    14581 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    14641 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    14701 ttctcttttc gttgtttgcg gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    14761 gcagattaat tttttaattt attgtttcta aaaagattat atctttatgt ccgtaaacgg
    14821 agatttcccg caaagccgat ttaccgataa tgaaatatcg ccttttataa ggatatctaa
    14881 gatgcgtaaa acagtggctt ttggctttgt cggtaccgtg ctggattatg ccggacgcgg
    14941 cagtcagcgc tggtcaaaat ggcgtccgtc actctgtata taccagcaag aatcgttggt
    15001 catcgatcga ctggaattat tgcacgatgc ccgctcgcgc tcgctatttg aaacgcttaa
    15061 acgcgatatc gccagcgttt cgccagaaac agaagtggtg ggcgttgaga ttgaactgca
    15121 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacga
    15181 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    15241 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaatcttc
    15301 aactccgcgc aaaaaagaac agccgcgcgg cccaggtgaa gtgacgatta tcgatctcga
    15361 tttaagccgt tataacgcca tcgccagccg ctttgccgag gaacggcagc aaacgctcga
    15421 tttcctcaaa tccggtattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    15481 cgaaaaagtg gcgattaaat cccgcgcgcc gattctgctt aacggcccga ccggcgcagg
    15541 taagtcattt ctggcgcgac gcatcttcga gttaaaacag gcacggcatc agtttagcgg
    15601 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgcgatgt cgacgctgtt
    15661 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggat tattacgcag
    15721 cgccaacggc ggaatgttgt ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
    15781 aatgctgctg aaagctattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    15841 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    15901 aggcaaattc cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    15961 tctacgccag cgccaggaag atattgaacc gaacctggat tatgaagtgg agcgccacgc
    16021 cacactcact ggtgacagcg tgcgttttaa caccgaagcg cggcgtgcct ggctggcctt
    16081 tgcaacatct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    16141 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggtgg aagatgagat
    16201 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    16261 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    16321 ctgccgccag gcaaagtcgc tttctgctgc cggacgacag ctttttgatg tttcgcgcca
    16381 gggcaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    16441 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
    16501 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatcaacca tgctgatgct
    16561 ccccatattg accatcgcat tacggccaaa ttttgaatgg tcaaccacca gcataacgtg
    16621 gcgcgagttc tcaataatgg cgcgcttggt acgaacttcg tggtaatcga actccagcag
    16681 cgagccgtcg ctgtcgatgc cgctgatccc cagaatgccg aaatcaaggc ggaactggga
    16741 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctacgta attcgccacc
    16801 ggcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacgt tgagattgtt
    16861 ggtgacaatg cgcaaattgc tgtgattgag cagcgcgtgc gccaccgctt ccggcgtggt
    16921 gccgatatcg ataaacagcg tcgagccatt ggggatttgc tccgccactt tgcgggcgat
    16981 gcgctctttt tcttcggtct gggtggcttt acgatcgtgc cacggcgtgt taaccgaact
    17041 ggaaggcagc gccgcgccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
    17101 gaggtcgcgg cgaatagtct gcgggctgac ggagaagtgc tccaccagtt cctcggtact
    17161 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    17221 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    17281 ctaaacccac ggctaatccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    17341 caaaccatcc ggcgacaatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    17401 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
    17461 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcatatag ccgcttaaca
    17521 gggcgctgat aagcgtaatg acaattagct taccgctacc gaggcgtttt tccaccgcgc
    17581 cgccgagata ccaccaccaa agcaggttaa agaggatatg catcagcgag aagtgcatta
    17641 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    17701 gccataacat cacttcctga tcgccgagaa tttgcatggc aataaacacc accacgcagg
    17761 cgatcatcat cacccaggtc accggacctg cgcgttcacg caaggcagca aagaaaggat
    17821 aacggcgata atgcaggcca ctgccggtat ggcctgccag ccagctggcc gccagataac
    17881 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    17941 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
    18001 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    18061 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    18121 gtacgccacc tctgcgggaa actgtcgctg ccaggcttca aagccgccgt caatgctata
    18181 gaccacatcg tagccctgtt gcagcagata ctgcgccgcc cctttgctgc tattgccgtg
    18241 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    18301 gtcgttggtt aaatggaaag cctgcaccgc atgccccata gcgaaactct gtggatcgcg
    18361 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    18421 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    18481 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    18541 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    18601 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    18661 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    18721 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    18781 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    18841 gaagtgctgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
    18901 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
    18961 ggtaaacgca ccagcctgcc gggatcaact ggtttgcgtt ttggcgcaaa ttcagtgtta
    19021 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    19081 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    19141 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    19201 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    19261 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    19321 agccatattg tggtgccgcg cgtgcatacc caaaagcagg cctacattct gcaaaacgaa
    19381 gataaacgca ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
    19441 gatgtggaat acaaaggtga tccgaaagca gtgaagattg aagagagtga aatcaattac
    19501 ctgctgaaag tgtataacgc gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
    19561 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca agccattacc
    19621 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    19681 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    19741 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    19801 gaaggcgacc gcgacgatta cgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
    19861 tcgctggcac gtcactacac gcgcacttac ggcagcaaca gcgagctgct gctcggcaat
    19921 gcgggaacgg taagcgatct cggggaagat ttcggtcatg agttctacga agcggaactg
    19981 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctgtg gcgtcgcaca
    20041 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    20101 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtgatc aggtcagatt
    20161 tcaatctggc ctgagactga tgacaaacac aaaactgcct gatgcgctac gcttatcagg
    20221 cctacgtggt ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc
    20281 acgtcgcatc cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc
    20341 agggaattac cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc
    20401 ccactcgccc cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca
    20461 gaaggtaaat tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag
    20521 cccccagcat ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag
    20581 tcattaccag cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg
    20641 aatggcgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
    20701 ggcgaaaaaa acgggaccct ttgg
//
LOCUS       DADLCQ010000070        20710 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000070 DADLCQ010000000
VERSION     DADLCQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.070"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQG95_002392"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JQG95_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY5888920.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQG95_002393"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JQG95_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY5888921.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQG95_002394"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JQG95_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY5888922.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQG95_002395"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JQG95_002395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY5888923.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8241
                     /gene="elyC"
                     /locus_tag="JQG95_002396"
                     /pseudo
     CDS             7461..8241
                     /gene="elyC"
                     /locus_tag="JQG95_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
     gene            complement(8218..9111)
                     /locus_tag="JQG95_002397"
     CDS             complement(8218..9111)
                     /locus_tag="JQG95_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY5888924.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="JQG95_002398"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="JQG95_002398"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY5888925.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /locus_tag="JQG95_002399"
     CDS             complement(10008..10190)
                     /locus_tag="JQG95_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY5888926.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="JQG95_002400"
     CDS             complement(10242..11474)
                     /locus_tag="JQG95_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY5888927.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="JQG95_002401"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="JQG95_002401"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY5888928.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="JQG95_002402"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="JQG95_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY5888929.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="JQG95_002403"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="JQG95_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="JQG95_002404"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="JQG95_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY5888930.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="JQG95_002405"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="JQG95_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY5888931.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="JQG95_002406"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="JQG95_002406"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY5888932.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19833..20594)
                     /locus_tag="JQG95_002407"
     CDS             complement(19833..20594)
                     /locus_tag="JQG95_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5888933.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASG"
BASE COUNT         4983 a         5725 c         5030 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgcaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa caacgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcta ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tagcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttagccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccaggctag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaagagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacgaacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat
//
LOCUS       DADLCQ010000071        20570 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000071 DADLCQ010000000
VERSION     DADLCQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.071"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            409..1239
                     /locus_tag="JQG95_002408"
     CDS             409..1239
                     /locus_tag="JQG95_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY5888934.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQG95_002409"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="JQG95_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY5888935.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="JQG95_002410"
     CDS             complement(2368..3828)
                     /locus_tag="JQG95_002410"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY5888936.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQG95_002411"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="JQG95_002411"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY5888937.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="JQG95_002412"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="JQG95_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY5888938.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQG95_002413"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="JQG95_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY5888939.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQG95_002414"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="JQG95_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY5888940.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQG95_002415"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="JQG95_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY5888941.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="JQG95_002416"
     CDS             complement(9005..11641)
                     /gene="fimD"
                     /locus_tag="JQG95_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY5888942.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIYGIARGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="JQG95_002417"
                     /pseudo
     CDS             complement(11708..12433)
                     /gene="fimC"
                     /locus_tag="JQG95_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
     gene            complement(12470..13009)
                     /locus_tag="JQG95_002418"
     CDS             complement(12470..13009)
                     /locus_tag="JQG95_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY5888943.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13074..13622)
                     /locus_tag="JQG95_002419"
     CDS             complement(13074..13622)
                     /locus_tag="JQG95_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5888944.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="JQG95_002420"
     CDS             complement(14103..14699)
                     /gene="fimE"
                     /locus_tag="JQG95_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY5888945.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GNRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="JQG95_002421"
                     /pseudo
     CDS             complement(15177..15779)
                     /gene="fimB"
                     /locus_tag="JQG95_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            17233..17949
                     /gene="nanC"
                     /locus_tag="JQG95_002422"
     CDS             17233..17949
                     /gene="nanC"
                     /locus_tag="JQG95_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY5888946.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            17969..19075
                     /locus_tag="JQG95_002423"
     CDS             17969..19075
                     /locus_tag="JQG95_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY5888947.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            19140..20120
                     /locus_tag="JQG95_002424"
     CDS             19140..20120
                     /locus_tag="JQG95_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY5888948.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPTCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         5478 a         4908 c         4672 g         5512 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gaatgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg tagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc atcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtaga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg gcattaatac
    11101 cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata aatgcctgag
    11161 ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg tcttggacca
    11221 ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg acagaagccg
    11281 tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac
    11341 tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc aaatagatat
    11401 cgacgcgata cgtccctggc ggtaattctt gcccattttc aaaacgcgat aaatcggcca
    11461 cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca gatgacaaag
    11521 gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca gccaaacgat
    11581 gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc agatatgaca
    11641 ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc gaaaaattcc
    11701 ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa tcatttattg
    11761 ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt tcgcccattg
    11821 gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt accgtcaggt
    11881 aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat cttaattttt
    11941 ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt ttaatgcggc
    12001 tgataattgc gagctgtagc atattctcag tcaattttga tttatccatt gacggaatcg
    12061 ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta tttgttgcat
    12121 caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga ggcgtcacga
    12181 taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt aaataggtac
    12241 tattttcatc attatttgtc acggcaagtt gcacttgttt ttaccctgcc ggataaatta
    12301 cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc attgccatga
    12361 acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt acattgacgt
    12421 ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat tactgatagg
    12481 ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc ccggtagcac
    12541 gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc cagtttgctg
    12601 gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca ccaatattgg
    12661 aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca tccgcgacac
    12721 cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt aaatgaataa
    12781 caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg ctgatttgcc
    12841 ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc gcaataatga
    12901 cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt ccggccagag
    12961 caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc gttattttta
    13021 tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt aggttattga
    13081 tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc gccgattgca
    13141 taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt ttgtgcacta
    13201 aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg cacgccaacg
    13261 tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg cgtagcatca
    13321 attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc gcaatcattc
    13381 agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgcttcag gctagcggta
    13441 cgaacctgtc ccaactgaac ggtttgatca acagagcctg catcaactgc gcaagcggcg
    13501 ttaacaactt cccctttaaa gtgaatggtc ccaccattta ccgtcgtagt atcggccaga
    13561 gccgctgcgg aactgaggga cagagccgac agaacaacga ttgccagagt tttaattttc
    13621 atgctgcttt cctttaaaaa aactatttct aaatcgacat gggcagtcgt tctgtacact
    13681 ttgttttgtc aacgagtttg actgccaaca ctgcacagtt ttcccccaaa agatgaaaca
    13741 tttggggcca ttttgactca tagaggaaag catcgcgcac aaactttttc agtttatttg
    13801 ttggcttaat attctattat tatctttatt tatagatttt tatattgcat gaggtagttt
    13861 ttggagagaa gaatgaggaa gatgcgtcga gccacagaaa cgttagcttt acatatagcg
    13921 gaggtgatgt gaaattaatt tacaagagaa ataatttaca tatcaaatag ttagatgctt
    13981 tttgtcgttt tttaatattt ttatgcttga gaaaaaatac gtaacttatt tatgatatgg
    14041 acagtttggc cccaattgtc ttgtgtttat ttgtttttta aatttattat caataagtta
    14101 aatcaaacct cttctctttt taatttttcg tttatgagat tatttctttc ccataatccg
    14161 gcaaaacgag cagcattact ggcggtataa cgcacagtat ggcgaatatt tcgattcccg
    14221 agataatcct gaattaaacg ggtatctgca ccacgctccg ccagttcata accgcaagca
    14281 tgccttaaca tatgaggatg agtctgcgtt acggttccag cttcaatacc ggcatcgcga
    14341 ataatgcgat aggcctgctg gcgagaaagc cgactcccgc ggcgagaaat aaatatagcg
    14401 tcggtccggt cagcgccttt ccagttagca cgttcctggg tccagcgttc cacggcttca
    14461 cgctcatcaa aacgtaacgg gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt
    14521 attcgacctt cattaaggtc aaggtcctga taatgcagat caagcagttc actaatacgc
    14581 atcccatgcc gatatgccaa cagaataaga caataatctc tggctcccgt tgccccgtaa
    14641 caaaccgcct gcatcatggc ctgaacttct ttaccggtaa gataacgacg tttactcaca
    14701 atagtagtac tcctaactga gatatatttg aatgaatacc tataggaaac ctcaatcggt
    14761 caaaattagc ccaatagata aaaagataac cacacacgaa gttatgtggc tatcagtcaa
    14821 ttacattaaa catcatacaa aaataaaata tcactgatgt gacaacatct attttcattg
    14881 atttgattca atataacaat agattacaga ggattaactt ccagttactt aaaaaataaa
    14941 gaaaaaagtg tggaaacagg agaatttaag tgatgtgtaa acttatgaat gattttgttt
    15001 atttattttg aagaacataa accattagat tttgtatggg tatggttaac ttaatgttta
    15061 ttatgataat taagaatcac acttacaata acatcaatta ataaatggtt aatcattttt
    15121 gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa ctatctctat
    15181 aaaacagcat gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa acgccctgca
    15241 ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata atcctagata
    15301 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatagcg taacatgtgc
    15361 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
    15421 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
    15481 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
    15541 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
    15601 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
    15661 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
    15721 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
    15781 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
    15841 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
    15901 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
    15961 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
    16021 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
    16081 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttaaac tagccgtctc
    16141 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
    16201 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
    16261 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
    16321 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
    16381 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
    16441 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
    16501 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
    16561 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
    16621 aaataaatta tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
    16681 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
    16741 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
    16801 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
    16861 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
    16921 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
    16981 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
    17041 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
    17101 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
    17161 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
    17221 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
    17281 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
    17341 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
    17401 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
    17461 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
    17521 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
    17581 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
    17641 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
    17701 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
    17761 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
    17821 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
    17881 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
    17941 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
    18001 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
    18061 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
    18121 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
    18181 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
    18241 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
    18301 ccccaaaacc aatagctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
    18361 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
    18421 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
    18481 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
    18541 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
    18601 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
    18661 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
    18721 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
    18781 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
    18841 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
    18901 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
    18961 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
    19021 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
    19081 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
    19141 tgaacgcaat aatatcaccc gattattact atgttcttac cgttgctggt cagtctaatg
    19201 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
    19261 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgacatgt cactttaacg
    19321 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
    19381 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
    19441 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
    19501 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
    19561 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
    19621 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
    19681 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
    19741 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
    19801 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
    19861 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
    19921 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
    19981 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
    20041 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
    20101 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
    20161 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
    20221 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
    20281 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
    20341 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
    20401 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
    20461 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
    20521 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DADLCQ010000072        20536 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000072 DADLCQ010000000
VERSION     DADLCQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.072"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            146..712
                     /gene="yqaB"
                     /locus_tag="JQG95_002425"
     CDS             146..712
                     /gene="yqaB"
                     /locus_tag="JQG95_002425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY5888949.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            709..1122
                     /locus_tag="JQG95_002426"
     CDS             709..1122
                     /locus_tag="JQG95_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5888950.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFCALVKRYAMLQLQQRPFRA"
     gene            1209..2765
                     /gene="gshA"
                     /locus_tag="JQG95_002427"
     CDS             1209..2765
                     /gene="gshA"
                     /locus_tag="JQG95_002427"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY5888951.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2915..3430
                     /gene="luxS"
                     /locus_tag="JQG95_002428"
     CDS             2915..3430
                     /gene="luxS"
                     /locus_tag="JQG95_002428"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY5888952.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3494..5032)
                     /gene="emrB"
                     /locus_tag="JQG95_002429"
     CDS             complement(3494..5032)
                     /gene="emrB"
                     /locus_tag="JQG95_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY5888953.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFDAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5049..6221)
                     /gene="emrA"
                     /locus_tag="JQG95_002430"
                     /pseudo
     CDS             complement(5049..6221)
                     /gene="emrA"
                     /locus_tag="JQG95_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(6348..6878)
                     /gene="emrR"
                     /locus_tag="JQG95_002431"
     CDS             complement(6348..6878)
                     /gene="emrR"
                     /locus_tag="JQG95_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY5888954.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6849..6950
                     /locus_tag="JQG95_002432"
     CDS             6849..6950
                     /locus_tag="JQG95_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888955.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(6969..7304)
                     /gene="ygaH"
                     /locus_tag="JQG95_002433"
     CDS             complement(6969..7304)
                     /gene="ygaH"
                     /locus_tag="JQG95_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY5888956.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7294..8031)
                     /locus_tag="JQG95_002434"
     CDS             complement(7294..8031)
                     /locus_tag="JQG95_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY5888957.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8155..9334)
                     /locus_tag="JQG95_002435"
                     /pseudo
     CDS             complement(8155..9334)
                     /locus_tag="JQG95_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9526..10518)
                     /gene="proX"
                     /locus_tag="JQG95_002436"
     CDS             complement(9526..10518)
                     /gene="proX"
                     /locus_tag="JQG95_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY5888958.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10576..11640)
                     /gene="proW"
                     /locus_tag="JQG95_002437"
     CDS             complement(10576..11640)
                     /gene="proW"
                     /locus_tag="JQG95_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY5888959.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11633..12835)
                     /gene="proV"
                     /locus_tag="JQG95_002438"
     CDS             complement(11633..12835)
                     /gene="proV"
                     /locus_tag="JQG95_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY5888960.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13190..14149)
                     /gene="nrdF"
                     /locus_tag="JQG95_002439"
     CDS             complement(13190..14149)
                     /gene="nrdF"
                     /locus_tag="JQG95_002439"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY5888961.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14159..16303)
                     /gene="nrdE"
                     /locus_tag="JQG95_002440"
     CDS             complement(14159..16303)
                     /gene="nrdE"
                     /locus_tag="JQG95_002440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY5888962.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRASPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16276..16686)
                     /gene="nrdI"
                     /locus_tag="JQG95_002441"
     CDS             complement(16276..16686)
                     /gene="nrdI"
                     /locus_tag="JQG95_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY5888963.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16683..16928)
                     /gene="nrdH"
                     /locus_tag="JQG95_002442"
     CDS             complement(16683..16928)
                     /gene="nrdH"
                     /locus_tag="JQG95_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY5888964.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17275..17505)
                     /locus_tag="JQG95_002443"
     CDS             complement(17275..17505)
                     /locus_tag="JQG95_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5888965.1"
                     /translation="MYNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            17657..18001
                     /locus_tag="JQG95_002444"
     CDS             17657..18001
                     /locus_tag="JQG95_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY5888966.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18038..18487)
                     /gene="alaE"
                     /locus_tag="JQG95_002445"
     CDS             complement(18038..18487)
                     /gene="alaE"
                     /locus_tag="JQG95_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY5888967.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            19155..19559
                     /gene="stpA"
                     /locus_tag="JQG95_002446"
     CDS             19155..19559
                     /gene="stpA"
                     /locus_tag="JQG95_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY5888968.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(19606..20130)
                     /gene="ygaP"
                     /locus_tag="JQG95_002447"
     CDS             complement(19606..20130)
                     /gene="ygaP"
                     /locus_tag="JQG95_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY5888969.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(20140..20439)
                     /locus_tag="JQG95_002448"
     CDS             complement(20140..20439)
                     /locus_tag="JQG95_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5888970.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         5200 a         5128 c         5254 g         4954 t
ORIGIN      
        1 ttctccaaaa aatttccttc aaaaatatct catttctaaa taactacttt atccatttcc
       61 tgaattttct tctccttcgc catcagcgac aaaatcaagc aattacgcta aagtagcgtc
      121 atatttttcg acttgcgaga agaccatgta cgagcgttat gcaggtttaa tttttgatat
      181 ggatggcaca atcctggata cggagcctac gcaccgtaaa gcgtggcgcg aagtattagg
      241 gcactacggt cttcagtacg atattcaggc gatgattgcg cttaatggat cgcccacctg
      301 gcgtattgct caggcaatta ttgagctgaa tcaggccgat ctcgacccgc atgcgttagc
      361 gcgtgaaaaa acagaagcag taagaagtat gctgctggat agtgtcgagc cgcttcctct
      421 tgttgaagtg gtgaaaagtt ggcatggtcg tcgcccaatg gctgtaggaa cggggagtga
      481 aagcgccatc gctgaggcat tgctggcgca cctgggatta cgccgttatt ttgacgccgt
      541 cgtcgctgct gatcacgtca aacaccataa acccgcgcca gacacatttt tgttgtgcgc
      601 gcagcgtatg ggcgtgcaac cgacgcagtg tgtggtcttt gaagatgccg atttcggtat
      661 tcaggcggcc cgtgcagcag gcatggacgc tgtggatgtt cgcttgctgt gagtgaagcg
      721 ttatcgcttt tctcgttgtt tgccagtagt tttctcagcg ctacactatt acccggcaac
      781 tccgaagtcg tgctggtggc aatgttgctt tccgggatca gtcatccctg ggttttagtt
      841 ttaacagcaa caatgggtaa tagccttgga gggttaacta acgttatcct tgggcgtttc
      901 tttccattgc gtaaaacatc gcgctggcaa gagaaagcta ccggctggct gaaacgctat
      961 ggtgcagtca cgctattatt aagctggatg cccgtggttg gcgatttact gtgtctgtta
     1021 gcgggatgga tgcgcatctc ctgggggcca gtaatctttt tttgtgcctt ggtaaagcgt
     1081 tacgctatgt tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg cactaattgt
     1141 aggcctgcac atatggtcac cattacagtt atgctaatta aaacgatttt gacaggcggg
     1201 aggtcaattt gatcccggac gtatcacagg cgctggcctg gctggaaaaa catcctcagg
     1261 cgttaaaggg gatacagcgt gggctggagc gcgaaacttt gcgtgttaat gctgatggca
     1321 cactggcaac aacaggtcat cctgaagcat taggttccgc actgacgcac aaatggatta
     1381 ctaccgattt tgcggaagca ttgctggaat tcattacacc agtggatggt gatattgaac
     1441 atatgctgac ctttatgcgc gatctgcatc gttatacggc gcgcaatatg ggcgatgagc
     1501 ggatgtggcc gttaagtatg ccatgctaca tcgcagaagg tcaggacatc gaactggcac
     1561 agtacggcac ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc
     1621 gctacggcgc gctgatgcaa accatttccg gcgtgcacta caatttctct ttgccaatgg
     1681 cattctggca agcgaagtgc ggtgatatct cgggcgctga tgccaaagag aaaatttctg
     1741 cgggctattt ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt ccttatctgt
     1801 ttggtgcatc tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt
     1861 ttgagaaaac cgagtgcggt atgtattacc tgccgtatgc gacctctctt cgtttgagcg
     1921 atctcggcta taccaataaa tcgcaaagca atcttggtat taccttcaac gatctttacg
     1981 agtacgtagc gggccttaaa caggcaatca aaacgccatc ggaagagtac gcgaagattg
     2041 gtattgagaa agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg
     2101 aactgtatgc gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg
     2161 cgctgttacg tggcggcatt gaatatattg aagtgcgttc gctggacatc aacccgttct
     2221 cgccgattgg tgtagatgaa cagcaggtgc gattcctcga cctgtttatg gtctggtgtg
     2281 cgctggctga tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc gttaactgga
     2341 accgggtgat ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg
     2401 cgcagttccc gttactgcag gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc
     2461 aaacgctgga tagcattaac ggcggcgaag cgtatcagaa agtgtgtgat gaactggttg
     2521 cctgcttcga taatcccgat ctgactttct ctgcccgtat cttaaggtct atgattgata
     2581 ctggtattgg cggaacaggc aaagcgtttg cagaagccta ccgtaatctg ctgcgtgaag
     2641 agccgctgga aattctgcgc gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc
     2701 gtcagcagga aatggaaacc gctgataccg aaccgtttgc ggtgtggctg gaaaaacacg
     2761 cctgacagaa aagaaaaagg ccactcggga gtggccaaaa tttcatctct gaattcaggg
     2821 atgatgataa caaatgcgcg tctttcatat actcagactc gcctgggaag aaagagttca
     2881 gaaaattttt aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata gcttcacagt
     2941 cgatcatacc cggatggaag cgcctgcagt tcgggtggcg aaaacaatga acaccccgca
     3001 tggcgacgca atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc
     3061 agaaagaggg atccataccc tggagcacct gtttgctggt tttatgcgta accatcttaa
     3121 cggtaatggt gtagagatta tcgatatctc gccaatgggc tgccgcaccg gtttttatat
     3181 gagtctgatt ggtacgccag atgagcagcg tgttgctgat gcctggaaag cggcaatgga
     3241 agacgtgctg aaagtgcagg atcagaatca gatcccggaa ctgaacgtct accagtgtgg
     3301 cacttaccag atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg
     3361 tgaggtacgc atcaacagca acgaagaact ggcactgccg aaagagaagt tgcaggaact
     3421 gcacatctag tcagtaaact atcttcacaa ttaattgaaa aaagccagtt caaatgaact
     3481 ggcttagttg tacttagtgc gcaccgcctc cgccgccacc tgcgccaaat ggcggtttag
     3541 caaaccacac cagccccagc agtacgagga atatcccggc tgacatccag aagatctcat
     3601 tggcggaaat aatcagcccc tgattggtga tctgctgggc aatccagcct gatgcctgct
     3661 gttgcgtcat cccaagccct tccagttgac tgtacatcgc ctgggcattc gggttgaacg
     3721 ggtttaccga ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg
     3781 tggttatcga cgtgccgata gaccccgcca gcgttcgcgt aaagttagag aggctcgatg
     3841 ccgccgccag tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga
     3901 agcagaccac cgcaaacccc tggataaact gcggccaggc cgacgcgcaa aaatccatac
     3961 ctggttcaaa ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg
     4021 ttaccagccg ccgcatatcc agtttatgcg cgaagcggcc gataatcggc gacagaatca
     4081 ccggaataat ccctaccggc gcagaggcca aacctgccca ggtcgccgtg taaccgtaga
     4141 cctcctgcaa caactgcggc agcagaacaa tagcgtcgaa gtagagcata tacgcgaggc
     4201 tgatacacaa gcagccgatg gtgaagttgc gcgacttaaa caacgacaga tcgactatcg
     4261 ggttatcgtc ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg
     4321 taagaatgat aatttcctgt gatgaaaaac agtccagctc tttaccgcgg tcgagcataa
     4381 tctgcaggct gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc
     4441 gttcggtgcg ggtttcacgt ccgcgcaggg tttgcagtgt catcaacacc accgccacgc
     4501 caatcggcac gttgatgaag aatatccagc cccagtggta attatcgctg atataaccgc
     4561 cgaggatcgg gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga
     4621 tcgagcgttt ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcaacg
     4681 gcccggcgac aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga
     4741 caccacacgc ccaagacgca atagcaaagg cgatggtgga ccaaaggaac agtttcactt
     4801 ccccgacgcg ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga
     4861 aagaagtgat tacccacgtt ccttggctga gcgatgagcc cagattcccg gcgatagtgg
     4921 ggatcgccac gttagcaatg gtggagttca gcacctgcat gaatgtcgcc agtgacagcg
     4981 caatcgtcat aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca
     5041 cctctggatt agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc
     5101 aggctgattt cacgcgcggt acttaccgcg accggtgttg aacgtacttt atttgccagt
     5161 acctgaccgt cacggttagt ggtattgacg ctcaccagcg tggacaaacc aatgcgcagc
     5221 gggtattgct ccagctgttt ctggtccagt tcgatacgca caggtagacg ctgaacgact
     5281 ttgatccagt taccggtcgc attttgcgcc ggaagcagtg agaacgcgct acctgtgccc
     5341 atatccagac caaccacttt accggtgtat ttcacatcat cgccgtaaat atcggtggtg
     5401 atggtgaccg gctgaccgat gcgcatattg gcaatctgcg tctctttaaa gttggcatcc
     5461 acccacatat tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgca
     5521 cccggttgca cggcacgacg ggaaacataa ccggtcatcg gactgacaat acgggtacgc
     5581 tccagcgcca gctaggcgtt acgtacttcg gtggcagcct gttgcacggc aggctgatct
     5641 tccagtttag tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc
     5701 agttgcgcct gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc
     5761 aggttggcat tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt cgcgagggcg
     5821 attttttgca cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt
     5881 tggcgaacgc tggaagccag tgcagttttg gctttttcaa acgcctggcg agcatctgtc
     5941 gggtcgagag tgaccagaac atcgccttct tttacaaaat cggtgttatc ggcccagact
     6001 ttcgtcacgc tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg
     6061 tcatcggttt cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca
     6121 ataattataa agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt
     6181 accggttgct gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc
     6241 ttaactcatc ggctgagccg acctgcttgc caaaaaggcc agcatgttgc gatgctggcc
     6301 ggtcattttt tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct
     6361 tcgagaacca caccgtcttg ttccatctgg tcgagacggg agagcaattt gcgggtgatt
     6421 tgctcgagct gatctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta
     6481 tgctgcggtg gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc
     6541 aggcagcggc gatcgttatc gcttttacga cgttcgatcc aaccgcgttt ttccagttca
     6601 tcggcaatac gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa ttcagaaggc
     6661 tgaatactgt ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta
     6721 atcccctgag ccttcagcat tttattgcgg ttctccagca gcttgctttg catgtgcatg
     6781 caaagacgag tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat
     6841 tttagcattt gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt
     6901 cagccgatat agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt
     6961 cttgtgggtt atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc
     7021 agtgttggga tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg
     7081 accagcgtgg gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc
     7141 agcagagcgc atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt
     7201 ttggttgggc gggcattacc cacacgcagg cgcagcggca aatagcggaa gcaataattc
     7261 gccgcgccaa ccagtaaccc aagcagcaga acctcatagc tcatcgggcg ctccttgcca
     7321 gaatgcctgg attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg
     7381 aatagaaaat agcgttacgc ctgcaagggc accaactaaa gctgcggtaa cgcaaagaga
     7441 ttgtttgcgc tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc
     7501 taatgcagct tcaacggcgg gataaccttg cagcaagccg ctgccggaga atgcccctat
     7561 taccgtacca aatacccacg atgaccatga actgaaggca atgccgatca tccagttctc
     7621 gctccagcgg cgattattgc gtaccagttt tgcggtggcg gcggcaaaaa cctcatccgt
     7681 caggccaaac gcccataggg cggttttcga tttttgcaga cgctgaataa tacggctacg
     7741 cagtgacggg ccatacaaaa catggcgaac atccattgcc atgacgctca gtgcagcaac
     7801 ccacaaacta ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata
     7861 aatgatgcag gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag
     7921 accgaacgca aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc
     7981 ttccatgaag gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac
     8041 gcttaacgat tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga
     8101 tgggcttctt aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt
     8161 tgcggcttcc tgacgatgaa aacctcgcca ccagaccagt aagttaacca gggcaatcgt
     8221 cgcgccagcc agacaaacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag
     8281 cgaaccggcg gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg
     8341 cgcatcagga tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgtacggt
     8401 gaggtccagc accagaatgc cgataatcaa cgccagtacg gaagtgtgac caaaccagat
     8461 tgccagccat gaaagtaaca gcagaagcag accgaaagtt gtggtgcgat ttgcccttgt
     8521 cggcaaaacc gcccgccgga cgagcgccca acgctccggc agctcccgca agcccaaaca
     8581 gaccaatgtc accatcgctg tagttaaaag gtggagcggc aagcaaaaag gccattgagg
     8641 tccagagaat gctgaagttg gcaaaggtca tgcagcccag caacgcgcgg gtgcgcagga
     8701 tcttatcgct gatgaacatg ctgaaaacgg aacccaacaa ctgtgggtag ttgaggtggg
     8761 tttctgattt catttgtggc agaccacgcc ataatgccag cgccatcagt gccattaaca
     8821 ccgaagcaac ccaaaagacg gtgcgccagc cgccgaggtt tgccagtaac ccggcaacgg
     8881 ttcgtgccag caatatcccc aacagcagac cgctcataat ggtgccaacc actttgccgc
     8941 gtttatccgg tgaagccagc gtcgctgcca gcggaaccag aatttgtgcc acgactgaga
     9001 ataaaccggt taatgccgta ccgaggatca tcatcgccag tgactgactg ctggcagtga
     9061 ttaacatacc gcccgctgcc agtaaggtca tcgagacaat caggcggcgg cgttcaaaca
     9121 tatcaccgag gggaacaaga aacagtagac ctgcggcata gcccaactgc gcggcggtaa
     9181 caataaagcc tgccgaactg gcggaaaggg aaaagttacg cgcgatggtg tcgagcaatg
     9241 gctgggcgta atagttactg gctaccgcca gaccggtggc gatagacatc agcacgatca
     9301 gcgccgggct aagctcatga ttaggtttag tcattatttt tgttcatcag gtgatgatta
     9361 agctggaaat caataatagc ggatgttgat gaatatggga tgaaagatag cttgttgaaa
     9421 tgtgcgatgt ggtttgtagg catgatagga cgcgtcagcg tcgcatcagg cattgtgtac
     9481 agagcggcgg atgaggcgaa aacgccttat ccgcccgaat aaaaattact tctgcgctgc
     9541 cagcgcctca ttcacccagc catcgaactg ctgctggtgg gctttgatcc agccatcaac
     9601 gtggccctga atatcgcctt ctgaggcttt gccgtcatgc ataatggcgt tctgggcgtt
     9661 aatatcggcc actggcaact gcataatggc aaacagtttg gctgccgccg gatttttctc
     9721 ggtccaggct ttgttggcaa cgatatgcat ggtgctgacc gggaagccgt aattcgcgcc
     9781 attcggcagt ttggtatcgg catttttatc gcccggcagt gcggagaacg gcacctgcaa
     9841 ccagaccaca tctttccctg gcttcagctc attactcacc cagtacggcg tccaggtgta
     9901 ataaaacacc ggtttgccct ctttgtagcg actgatggtg tcggccatca tcgctgcgta
     9961 gttcccctga ttatgagtca cggtgttggt cagttcatac gcggcaagct ggtggttgat
    10021 cgcaccttcg cagccccagc cagggttaca accggttaaa tccgctttac cgtcgccgtt
    10081 ggtatcgaac agtttggcga tcttcggatc tttcagttgt gcgatgttgg tgattttgta
    10141 ttggtcggcg gttttcttat cgatcaggta accctgtgct gcgccgttaa caaatacccc
    10201 ttcacgataa aatttcttat cgccaccggc agcttcgtac atgttgtcat ggagtggcgt
    10261 ccagttcacg gcggtgaagg ttgcatcgcc ggaagcaagc gaggtgtagc caacgttgta
    10321 atccacttcg ctgggtttgt tgacggtata acctaatttc tccagcgcac gactgaccag
    10381 cagcgtctgg aaggtttctt cagtgatggt gctctgaact ggattaacag taatgccttt
    10441 gcccggcaga tcggcagcaa aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa
    10501 aagtacgcta tgtcgcattg ttattccttt ttttggcagg gtgataatgc ccggcgccac
    10561 cgggcaagtg cagagttact taatgaatgg gcgggtcagc agaccaacag ggccagtggt
    10621 gtaccagcga cggttgccgc gactgcgtga gtcgcgccca acggcctgcg tcaggcgatc
    10681 gaggataatg gcgaggatca caatcccgac gccgccaacg gtggcaagcc ccatatccag
    10741 acgaccgata ccgcgaagta ccatctgacc cagcccgccg acggcaatca tcgaggcaat
    10801 gaccaccata gaaagggcca gcatcagcgt ctggttaacg cccgccataa tggtcggcat
    10861 cgccagcggt aactgaactt tgaacagcat ctggcgcggg ctggcaccga atgagcgcga
    10921 ggcttcaatc agatccgccg gaacctggtt aatccccaga atggtcagac ggataatcgg
    10981 cggcagcgca aagatgatcg tcaccaccac gcccggcacg ttaccgatac caaatagcat
    11041 gacgattggc accagataaa caaacgctgg cgtggtctgc atggcatcaa gcagtggacg
    11101 aataattttc gccgctcgcg gacttctcgc cagccatatc cccaacggca aaccgatgac
    11161 gatacagaac agcagggcag ttaacaccag cgccagagtc accattgcct gcgaccaggc
    11221 accgattgcg ccgatggcaa tcagcgagac cagcgtcgcc acgcccattc cgaccccgga
    11281 aatctgccag gtgatgagag cgaaaacgat aatcgccact ggcgctggca tacccagcag
    11341 caattgctgg aaaccgttga ggatataatc aaccggaacg cgcacgccct ggaagacggg
    11401 acggaaatgg gtaacaaccc agtcgatccc ttcagtgacc caactgtcga gcgggatcag
    11461 cgttttatgg aacggatcga gaatattaaa atgctcgacg tttggcgcag gcgtactggt
    11521 cagccagtca gcaccaccgc cgtcagccgg tgcagtcgtc ggtgtacccc aggcgtctgc
    11581 ggattgcgcg gcactgtccg ccgctggcgt ggtatcccac ggattatttt gatcagccat
    11641 tatttacccc ctcacgatct aaagcgcgca gcagcattcc tttcgaaatg atgccgacat
    11701 actgttggtc ctcgtcgacc acgggcaccg cacagggtgc ctgtccgaca tgagagagca
    11761 actcgctaag aggcgtttgt gcatcgactg ctaacggcgc atcaatcagc gccgcatcaa
    11821 gaccttgctg ctgcgttaac gcggttttaa gcgaatcgat ggagactgcg ccgacaaact
    11881 tattaccgcg ttcgataacg tagccatatt cgcgatcttc atcctgcaat aatttcagtg
    11941 ccgaacgtgg gccgaagcca ggagttttac gaattaagcc attcggtgtc cggcgggcaa
    12001 tatctttcgc actgaacacc tgactaatat caacgccacg gaagaaggta cggacataat
    12061 cattcgccgg attattgaga atttcatccg gtgtgccgac ctgtaccact tcaccatttt
    12121 gcataatggc aattcggtcg ccaatacgca tggcttcatc aagatcgtgg gaaataaaga
    12181 caatggtgcg ctgatgtttc gcctgtaatt ttaccagctc atcctgcatc tcggtgcgaa
    12241 ttaatggatc gagcgccgag aaggcttcgt ccattaataa tatatccgga ttaatcgcta
    12301 acgcgcgggc taatcccaca cgttgacgca tcccgccaga gagttcatcc gggtagctgt
    12361 gggcataatt ttccagcccg acctgacgca gtgcatcaag ggctttttcc cggcgttctt
    12421 cggcattaat tccggccaat tccataccga acgcagtatt gtccagcacg gtcatatgcg
    12481 gcattaaggc aaaggactgg aagaccatcg caatcttttt tctgcgcacc tcacggagtt
    12541 cggcgtcgga tattttggca atatccacac catcaatcag cacttgcccg cgggtgggtt
    12601 caatcaggcg attgagaagg cgtaccattg tggatttacc cgagccggat aatcccatga
    12661 tgacaaatat ctcgccttct tcaatggcca gactggcgtc ttttacgcca agcgatagcc
    12721 cagttttttc cagaatttgt tcttttgaaa gtccttgttc gatatatttg aacgctcgct
    12781 gtggatgctc accaaatatt ttataaagat ttttaatttt taatttaatg gccatgcaat
    12841 agaatgattc ctttatttgt ttatgtcgat atattaccca atgggaatag tcactttttt
    12901 ctaccctaac atactgagaa tctgaggcaa cccctgaggg caaatgtggc atgaatattg
    12961 atgcgagcaa atcgccggaa ttttccgtga tataagggct gagagcaaat cgagaaaagt
    13021 tgatattttt tgtcatagat agcgaaaaaa cgcctgaata ttgtagtttc aggcgaataa
    13081 tgcaacagag tatcagtgta gatcactaca gaatatttgc gtgataaaaa gctatttggc
    13141 gggaattatt tccctgctgc gggtagtgat atttttgaaa ataacaccct cagaaattcc
    13201 agtcttcatc ttctgtttca accgctttcc ccatcacata agaggagcct gaaccggaaa
    13261 agaaatcgtg attttcatcg gcattcggcg aaagcgcggc gaggattgcc ggattcactt
    13321 ccgccatttc tgcgggaaat aacggttcgt agcccagatt catcaaagcc ttattggtgt
    13381 tgtaacagag aaacgctttc acatcgtcag cccacggggt ttcggcgtac agctcatcgg
    13441 tgtattgcaa ctcgttgtcg tagagttcca gcaacaaatc gaaggcgaaa ctcttcaact
    13501 cttcacgttg tcccagagat atcttttcca tgtttttctg atatttatag cctatgtagt
    13561 aaccgtggac tgcttcatcg cggataatca gacggatcag gtccgcggta ttggtcagct
    13621 ttccgcggct ggaaaaatac atcggcagcc agaaaccgga atagaacaaa aaagattcaa
    13681 gaaacacact ggcgattttc tttttcagcg gatcatcacc gcgataatgt tgctgaataa
    13741 tctgagcttt tcgctgcaac ggtgcgtttt cttcactcca ggcgtaggcg gcatcgacat
    13801 ctttggtctg gcacagcgtc gagaaaatcg aactgtaaga gcgggcatga accgcttcca
    13861 taaagctgat attcgataat accgcctctt catgaggcgt gagcgcatcg ggcatcagcg
    13921 aaggcgcgcc gataacattt tgcagcgtgt cgagcagcgt caggccagta aaaacgcgca
    13981 tcgtcagttg ttgttctacg acagttaatg tctgccaggc gggaatatcg ttcgacagcg
    14041 gcaccttttc tggtagccag aaattgctgg tcaggcgatt ccacacctcc agatctttat
    14101 cgtcagatat cttgttccag ttgatggcgc tgatacgtga gagtttcata gatattcctt
    14161 aaagtgcaca ggagacgcag ccttcaattt cagtgccttc cagcgccatc tgacgcaggc
    14221 ggatgtaata gagcgttttg atacctttgc gccaggcgta aatctgcgct ttgttgatat
    14281 cgcgagtggt ggcggtatcg gggaaaaaca gcgtcagcga cagcccctga tcgacatggc
    14341 gagtcgcttc cgcgtaggtg tcgatgatct tttctgcgcc aatttcgtaa gcgtcctgat
    14401 acagcgccag attttcgtta gtcataaacg gggcagggta gtaaacgcgt cctgttttgc
    14461 cctctttgcg tatctctact ttcgccacaa tcggatgaat actcgacgta gcatggttga
    14521 tataagagat agacccggtt ggcggcaccg cctggagatt ctggttgtat atgccgtagc
    14581 gcatcacgtc gtcgcgcagt tgcgcccaca tctcacgggt aggtaacgta atacagctgc
    14641 gggcaaacag ttcgccaact ttcgccgttt tcggctgcca gttaccttgc agatattggc
    14701 tgaaatattc accactggca tagcgtgact gtttgaaccc ggcgaaggtt tcaccgcgtt
    14761 cgcgcgccag caacatcgag gtacgcagtg cgtgccaggt gatggtatag aaatagagat
    14821 tggtgaaatc cagggcttcc ggcgaaccat aagcgatgcc ttccctcgcc agataaccat
    14881 gtaaattcat ctgccccagt ccgatggcgt gcgaggcggc atttccggct tcgatggacg
    14941 gcacgctgcg gatatgactc atatctgaca ccgccgttaa accgcgcacg gcagtctcta
    15001 ccgtgcggga aaaatcgggg gaatccatgg tgtgcgcaat attcaacgaa cctaaattgc
    15061 aggaaatatc atggcctgtg cgggcatagt cgagattctc gtcatactct gaggcgctgt
    15121 taacctgcaa aatttctgag cagagattac tcatatttat gcgcccggcg atagggctag
    15181 cacggtttac tgtgtcttca tacatgatgt agggatagcc ggactcaaac tggatttctg
    15241 ccagtcgctg gaagaaatca cgggcgttga ggtatttttt gcggatgcgt tcatcggcaa
    15301 ccagttcgtc atagtgttcg ctaatggcga tatcggcaaa cggcttgcca taaactcgct
    15361 caacgtcata gggcgaaaac agcgccatct gcgcattctc ttttgccaga tggaaagtga
    15421 tatccgggat caccacgccc agcgacagtg ttttaatgcg gattttttcg tcggcatttt
    15481 cccgtttcgt gtcgagaaaa cgcagaatat cgggatgatg agcatgtaaa tagactgcac
    15541 ctgccccctg acgcgcgccg agttggttgg cataggaaaa tgcgtcttcc aacattttca
    15601 tcaccggaat tacgccagaa gattgatttt caatacgttt aattggcgcg cccgcttccc
    15661 gcagattcga cagcaaaaat gctacgccgc cgccgcgttt cgacagctgc agtgcggaat
    15721 ttaccgcccg accaatcgac tccatattgt cttcaatacg cagcaaaaaa caggaaacca
    15781 gttcgccgcg ctgctgctta ccgcagttga ggaatgttgg cgtggctggc tgaaagcgtc
    15841 ctgacagcat ttcatcggtc agttgcaacg ccagcgtctc atcgccttgt gccagcgtca
    15901 gcgccaccat cgtgactcga tcggcaaaat cttccagata acgtttaccg tcgaatgtct
    15961 tcaacgtata gctggtgtaa aacttccatg ccccgaggaa tgtctggaaa cgaaaaccgc
    16021 tggtgtgtgc gtgggcaaac agcgtaatga caaagtcgcg agagtagcga ttaagaacgc
    16081 tttcatcata gtaaccttcg ttgaccagcc agttcaggcg ctgctgctgg ctactgaagg
    16141 tcacactgtt cggacgcaca tgcgtcgcaa taaaggcgtc aacggcctgg cggtctttat
    16201 cgaactgaat gcgacctgcg ctatcgtaaa gattaagcat cgcattcagc gcgtggtaat
    16261 ccatcgtttc ctgcgtcagg cattctgcgg ttgtcgttgc caaaattcgg ttactccttt
    16321 acgaacgttt tcgatatcgc tttgcgtacc catgagttca aaacggtaca gccacggcac
    16381 gccgcatttc cgggcaatca catctccggc gcggccatac gcctcaccaa agttgcgatt
    16441 accagaagca ataacgccgc gaagcaacgc ccggttgtgc tcgtcgttta aaaagcgaat
    16501 tacctgtcgt ggcaccgcgc cagccgtacc gccgccgccg taagagggca cgatcaggat
    16561 gtaaggctcg tctacctgaa tccgttcccg ctcattgagc gggatgcgca ccgcgggcag
    16621 acctaaacgt tcgataaaac gctgcgtgtt ttcggagctg ctggagaagt agacgagctg
    16681 gctcatgcac tggccgcgtg tggcgctgga tgcagacggt taatcatgtc cggacggaaa
    16741 ccagaccagc taagatcgcc agcaatcact accggcaact gacgaaagcc ctgagcacgc
    16801 aacgcttccg ctgcttcagg aacgcgatcg acattaatca tttcaaaatc aaagccccgg
    16861 ttttccatcg cccgtttggt ggcgtggcac tgaacgcaat cgttacgagt ataaatagtt
    16921 atgcgcatga ttcgtatttc cgtttaaaat gaagatacgg cgcgatgata cgcgtcgggt
    16981 tgtctctctg ttgatacaga gatactagat gtagttgaaa aaagattcaa ccacacaata
    17041 tatagcaaat agtgatcgaa attaccctgg atatgagcgt gacgggggag ggggattttt
    17101 gtgattcacc aggcaaaaag aaaccccgaa gacaggcttc ggggtcaaag acgcgtattt
    17161 attatcattt ttgcactacg atttgcgcac gcttaacagt gcgccgataa aaatacctac
    17221 cgcagctgct gtacccacgc tacaccaggg tctttcacga acaaaagaat ccgctcagcc
    17281 aacggcatcg cgcgcggctt gctggacgcg agtacgacca tgcattcgcg ccctggtttc
    17341 tttcagcaac gcctgagctt tactgcgtgc ggcttcagct tcccctttgg cgtcgctgcc
    17401 ccaggacttc aatacagatt cgaggctgtc agctaattga ttgacatcat tctggatatc
    17461 ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta tacatatgat cccccattag
    17521 aattcgatgt aattttcagt gtagaccata attttcttaa ctgaggttca tcgcggcttt
    17581 atggactttt ctgaaagcgt tgtgaatgcg caatcaggta aggtaggccc gcggttgaaa
    17641 ataacgagga tcaaatatgt atttacgacc agacgaggtg gcgcgcgtac ttgaaaaagt
    17701 cggttttact gtcgatgtgg taacccagaa agcgtatggt taccgccgtg gggaaaatta
    17761 tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgct ctggtaattc atccaacatt
    17821 aaaagaacgc agttcaacac tggctgaacc cgcttccgat attaaaactt gcgatcatta
    17881 ccagcaattt ccgctctatt tagcaggcga gcgacacgag cattacggta tcccgcatgg
    17941 ctttagttcg cgtgttgcgc ttgaacgtta tttgaatggt ttatttggcg aagccagtta
    18001 aagacgcgac tggcgatgcc agtcgcgaaa agaagagtca ggcttttacc tgctggtaac
    18061 ggctgacttt aaacagtcgg cggcaataat cgaggaagta gccataaacc gcccccatca
    18121 acatcgaaac aacgatgttt gaactgaccg ccgccataat ctgatgccag tctgcgccca
    18181 ccactaacaa gatcgccaca tacaccggtg actggaacgt cacataagcc aggatatcag
    18241 ccagattttt tatccagccc gacgggctaa ctttgcgtgc cgcgcgcata aacagatcgc
    18301 ggtacatacc gtatggccat gcaattaaga tgttcaccgg aatcgctacc aatctggaat
    18361 aaaaagactg ttcgaagctc attccggaga ggaaaacttc aatacacatg ttcacgacag
    18421 aacagtaaac aaccatcgcg aacgtatctg caactgcatg acgcaagcgt gactgcggtg
    18481 agaacatgct aatgctcctt aataaaagcg taatgggatg ttaatggaga tgaaagcagt
    18541 ggatctcatt gccatctgca tttttgcata gcgtatatcg ctgtctatga ctaatcaatt
    18601 agtgaaaaat ttttatttag ttagtttttg tagataaaat attccgtaac ggctcgtttt
    18661 ttgtacattg tggattttta catgtcgata tgatttattt tcaattaaat caattagata
    18721 tgatcttgcc tttaatttca ctaatgaaag tttggggggg aggtaacgct ataagcgtag
    18781 agaattcggt gtttgtcgtg accgggtcat ttacagcatt tttgatgcca gaaaatgtac
    18841 attaacagca gaaagcacca gtgaggggtg ttttctttta ttctgttata ttgcattttt
    18901 attcacttcg gttttgtcag gcttgcggag ttagcgagca gagagcgcca ctctgttcac
    18961 caataaccta aattactcat caaaatggct aatatataat cttgaaatta ttctgagagt
    19021 ttcagaaaat aacagctgaa ataatctcgc gcaggactgt aaatagatta aattttgtgg
    19081 aaatataata agtgatcgct tacactacgc gacgaaatac ttttttgttt tggcgttaaa
    19141 aggttttctt tattatgtcc gtaatgttac aaagtttaaa taacattcgc accctccgtg
    19201 cgatggctcg cgaattctcc attgacgttc ttgaagaaat gctcgaaaaa ttcagggttg
    19261 tcactaaaga aagacgtgaa gaagaagaac agcagcagcg tgaactggct gagcgtcagg
    19321 aaaaaattag cacctggctg gagctgatga aagctgacgg aattaacccg gaagagttat
    19381 tgggtaatag ctctgctgct gcaccacgtg ctggtaaaaa acgccagccg cgtccggcga
    19441 aatataaatt cactgatgtt aacggtgaaa ctaaaacctg gaccggtcag ggccgtacac
    19501 cgaagccaat tgctcaggcg ctggcagaag gtaaatctct cgacgatttc ctgatctaat
    19561 tactgaggcc ggatgtcgct gctagggcgc gtccggcgtc gcttctcaag ctcgttggtt
    19621 ccacggcatc ttatctaaca accttgccat tccacaaaaa ccgctgattc ctgcaaacag
    19681 taacccggca ccaacaaagc cacttaataa gaagaaaccg ctatttacgg tataacccag
    19741 tacaacgccg attaatatta aaccacccgc agcgatctgc acctggcgca ttaacggcaa
    19801 gggttgagat ttatttaccg ctactggcaa tccagctctt ttccagccat caatcccatc
    19861 ttcgagtaaa aatatttctg cgggggcggc aatcgctgct aatttatcgg cgttattact
    19921 ggtgcgttta cctgcctggc agtggaatat aatttgctcg cgacgtaatt taggtggaag
    19981 acctgactgt tccagcacgg ataatggagc cagatctgct tcaggaatat gttcacgaag
    20041 atattcatca gcgtcacgaa tatcgattaa ctttgcgccg cgtgcgatta attcttgtgc
    20101 atcatgcggc gaaatggttg tcaaagccat agtgactcct tacggacaat agacattttt
    20161 cagggtggcg ataatggcat ttaccgcctc atttttaatg gaatatagaa tgcgttgggc
    20221 atcccgttgg ctgttgataa gcccttcgtc ccgcatacga gcgagatgct gtgatgtcgc
    20281 agaggcgctc agtccggtaa tgcgcgtcag ctctcccgcg ctggtgccgg gggaaccgct
    20341 aagcatgcac agaatcagca accgtttagg gtggctcatt gctttcaata aggccgctgc
    20401 ctgttcggta ctggcctgta attgcgcgag ttcagtcata tttatttaag tattttctaa
    20461 attaagtaaa ctctaaacta aaaatgcaac atataccagc ctcagcagcg taaatgagag
    20521 taaaagcgta agctga
//
LOCUS       DADLCQ010000073        20521 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000073 DADLCQ010000000
VERSION     DADLCQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.073"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JQG95_002449"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JQG95_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY5888971.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JQG95_002450"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JQG95_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY5888972.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JQG95_002451"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JQG95_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY5888973.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQG95_002452"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JQG95_002452"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY5888974.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIHVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQG95_002453"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JQG95_002453"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY5888975.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JQG95_002454"
     CDS             4449..4814
                     /locus_tag="JQG95_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY5888976.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JQG95_002455"
     CDS             4964..5482
                     /locus_tag="JQG95_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAY5888977.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JQG95_002456"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JQG95_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JQG95_002457"
     CDS             6713..7195
                     /locus_tag="JQG95_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY5888978.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQG95_002458"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JQG95_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY5888979.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQG95_002459"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JQG95_002459"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY5888980.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQG95_002460"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JQG95_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY5888981.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQG95_002461"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JQG95_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY5888982.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQG95_002462"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JQG95_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY5888983.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JQG95_002463"
     CDS             10864..11730
                     /locus_tag="JQG95_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY5888984.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLMHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JQG95_002464"
     CDS             11751..13037
                     /locus_tag="JQG95_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY5888985.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQG95_002465"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JQG95_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY5888986.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JQG95_002466"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JQG95_002466"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY5888987.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JQG95_002467"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JQG95_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY5888988.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JQG95_002468"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JQG95_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY5888989.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JQG95_002469"
     CDS             complement(16985..17275)
                     /locus_tag="JQG95_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY5888990.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQG95_002470"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JQG95_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY5888991.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JQG95_002471"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JQG95_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY5888992.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JQG95_002472"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JQG95_002472"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JQG95_002473"
     CDS             19137..20378
                     /locus_tag="JQG95_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5888993.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5303 a         5358 c         4814 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctagaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacatgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacagattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acagacaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acattccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttaccta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgccttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcaacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctgatgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtaccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg caagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtattgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgctccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgagccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLCQ010000074        20287 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000074 DADLCQ010000000
VERSION     DADLCQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.074"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(60..818)
                     /gene="aroD"
                     /locus_tag="JQG95_002474"
     CDS             complement(60..818)
                     /gene="aroD"
                     /locus_tag="JQG95_002474"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY5888994.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="JQG95_002475"
     CDS             complement(849..1715)
                     /gene="ydiB"
                     /locus_tag="JQG95_002475"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY5888995.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLVCEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1727..2941)
                     /locus_tag="JQG95_002476"
     CDS             complement(1727..2941)
                     /locus_tag="JQG95_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888996.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVQFGERK
                     FSTRLNTIKHRG"
     gene            complement(3181..4386)
                     /locus_tag="JQG95_002477"
     CDS             complement(3181..4386)
                     /locus_tag="JQG95_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5888997.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4486..4842)
                     /locus_tag="JQG95_002478"
     CDS             complement(4486..4842)
                     /locus_tag="JQG95_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY5888998.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4978..5085)
                     /gene="rprA"
                     /locus_tag="JQG95_002479"
     ncRNA           complement(4978..5085)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JQG95_002479"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JQG95_002480"
     CDS             complement(5271..6383)
                     /gene="ydiK"
                     /locus_tag="JQG95_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY5888999.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6772..9828
                     /locus_tag="JQG95_002481"
     CDS             6772..9828
                     /locus_tag="JQG95_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY5889000.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVEGIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9825..10235
                     /gene="menI"
                     /locus_tag="JQG95_002482"
     CDS             9825..10235
                     /gene="menI"
                     /locus_tag="JQG95_002482"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY5889001.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10335..10523
                     /locus_tag="JQG95_002483"
     CDS             10335..10523
                     /locus_tag="JQG95_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889002.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11071..11439
                     /gene="sufA"
                     /locus_tag="JQG95_002484"
     CDS             11071..11439
                     /gene="sufA"
                     /locus_tag="JQG95_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY5889003.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11448..12935
                     /gene="sufB"
                     /locus_tag="JQG95_002485"
     CDS             11448..12935
                     /gene="sufB"
                     /locus_tag="JQG95_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY5889004.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12945..13691
                     /gene="sufC"
                     /locus_tag="JQG95_002486"
     CDS             12945..13691
                     /gene="sufC"
                     /locus_tag="JQG95_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY5889005.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13666..14937
                     /gene="sufD"
                     /locus_tag="JQG95_002487"
     CDS             13666..14937
                     /gene="sufD"
                     /locus_tag="JQG95_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY5889006.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDSLALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14934..16154
                     /gene="sufS"
                     /locus_tag="JQG95_002488"
     CDS             14934..16154
                     /gene="sufS"
                     /locus_tag="JQG95_002488"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY5889007.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16167..16583
                     /gene="sufE"
                     /locus_tag="JQG95_002489"
     CDS             16167..16583
                     /gene="sufE"
                     /locus_tag="JQG95_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY5889008.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16732..17736
                     /gene="ldtE"
                     /locus_tag="JQG95_002490"
     CDS             16732..17736
                     /gene="ldtE"
                     /locus_tag="JQG95_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY5889009.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17799..18035)
                     /locus_tag="JQG95_002491"
     CDS             complement(17799..18035)
                     /locus_tag="JQG95_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY5889010.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="JQG95_002492"
     CDS             complement(18346..19758)
                     /gene="pykF"
                     /locus_tag="JQG95_002492"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY5889011.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5314 a         4856 c         5176 g         4941 t
ORIGIN      
        1 ggttaaaaat acttttttta tttcggcggg ggagggtatt tccgccgaaa tattattgct
       61 tatgcctgat gtaaaatagt taataccgtg cgcaaatcat ttaccgagat ttgccctggc
      121 gcagaggctt tttttaccgc accaaaagtt gccgccgagc caaatacttc accaaccaga
      181 cgagaaatta cgccagtttt tgccatcgac atcgtgatga ttggacgatc ggcatactgc
      241 tcctgcatct ccagggtcgc ggcaagcaac gtcagcacat cgctggtact ttgcggcatc
      301 agcgcaatct taggaatatc ggcgtcgaag gactgcattt tgcgcagacg ggcaatgatt
      361 tcttcggctt ccggcgtttt atggaagtca tggttggaca tgacaacttt cacatcatgc
      421 gcgtgggcgt aggcgacggt ttctttgacc tgatcatcgc cggtaaataa ctccagatcg
      481 atcatatcaa ccaggccgct gtcgatggct gcacgattga gagcaatata agcctcggtg
      541 gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg taaacagcag cggtttttct
      601 ggcatggttt cacggagaat ttttgccgcc gccatgacag actccacatt ggagaggtcg
      661 gcaaagtggt ccacacgcca ttccagaata tcaaagtccg cttcacgata ggcgagagct
      721 tcggatttca cgcgggcgat atctttcgcc atcagcgaga cgatgatttt aggtgcgccc
      781 gcaccaatga cgagatcttt tacagttacg gttttcattt tttacccttt ctgcacgcgg
      841 tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat attccagagg gaaatctttg
      901 ccagtccata acgtgaactg ttcagcccct tgccacaaca acatgccgta tccatcaatc
      961 gttttgcaac cagcttgttg tgcctgctgc aataacttcg tcatatgcgg gttatacacg
     1021 cattcagtga ccagcagtcc cggatgtaac agactgatat cattaaccaa tgattcattc
     1081 tcaaggggtt tcatacctac ttttgtgcca ttggttaaaa tgtcggcgga agccagggct
     1141 tcagcaaagg cttgctgatc ggcgagatcg gtatccgtga cgacacaatc ggtgttttcg
     1201 ttaacccgct gcgcgaaggc gagggcttta tcgaagaact catcccgacg gttaaagagt
     1261 ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa tcgccgttga ggcacctccg
     1321 gcccctaaca gcaccatcgt tttgcctttg atatcaaaac cgctctcttt aatggcgcga
     1381 atatggcccg tgccgtcggt gttatagcca cgcagatagc catcatcatt aacgatggtg
     1441 ttgatggcac ccaccagttt ggctgctggc gttaattcat caacatattc acacaccagt
     1501 tgtttgttcg gcatcgacac accagttccg cgcattttga gggcttttaa tccttcaatt
     1561 gctccgggaa agctatcgtt atccacttcg aaggccatat aggtaaatgg caatcccgct
     1621 ttttctaagg ctttattctg catttcaggc gataaactgt ggcggatagg ataagccatc
     1681 aatccaatca attcgtattt tgcggtaaca tccataatta actcctttaa cctctatgct
     1741 taattgtgtt taaccgggtg ctaaatttgc gttcgccaaa ctgcacatca ttctcaggaa
     1801 taataaaaac gcggtaatag cggctaaaaa caataattgc ggtaatcagg gccagcagcg
     1861 cgaaagtaaa atcgagaaca atgatatatt gcaggccgat gttcgacaga taaccggtaa
     1921 tcagcggaat aacaaagtta gccagtccac ccatcatcat ataaatactg gtgactttgg
     1981 ctttgctttt ggggaaaaac tctgacatga ccgaaacacc gagctgtaaa atgccgccag
     2041 ctgctgagaa accgataaca aaggcgccgg cattacacac cagtggagaa gggtacaggt
     2101 agataatggc agctgttatt gttgccagcg cagagttaaa tacattagcc cagatgggcc
     2161 ggaccatttt tttcagtagt gcggcaaaaa taaagacaca gaccaacgag cccatactgt
     2221 aataagagat ggtttttaat gcctcagctt ctgacatacc agcaaaagcc atcgcatatt
     2281 tgggcatcca caccacaatc acataaaagg tcgagaatgc ggctacaccg aacagtaccg
     2341 atgaaacacc ttccagccag actaaaggtt tgctgttcat ttgcggtaat tcattagcta
     2401 cgctggcgtc caccaactgg ctggggaatt tgcttttcaa cagcatcagc gtgatcagta
     2461 caaacagaat acccggaata atcagcccat agccgtacca gatattattg agcaacatat
     2521 agctcaccag cattgggtag aacatttgcc caaatgacac catcgcttta accagtatga
     2581 ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc ggggtagcca cccgtatcca
     2641 gcgctgagtt agcgatacct acgcacactg ccagaccgta ggcgagagtt aaattcgggc
     2701 aagcgggaat accaaagaag aatagcagat acattattac tgccattaat atcaccgccc
     2761 gacgaccaaa cttatcggag atcacaccga agaataaaat actgatcaat cgccccaaac
     2821 cgataccgga aattaaatag gcaatgcccg cgttgtcagt ggaaaacttt tctgccagag
     2881 atgtcatatt ttgggcaagc gtaataacac taatgccgtg caggaaatag ctgaagtaaa
     2941 tacaaagaac agccaggata aatggcgtgc tgaaagataa tttcgttacc gtacgtattg
     3001 ttctccgacg gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata
     3061 aagtttatgt ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta
     3121 gttagcgcag aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca
     3181 ttacccaccc ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg
     3241 taagccgata aacagtgcca gtaaaaaacc gatggcagta tcgaaccaca taatatcggc
     3301 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3361 cgtactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3421 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3481 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3541 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3601 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3661 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     3721 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     3781 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     3841 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     3901 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     3961 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4021 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4081 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgtcaaaaa agaaggccat
     4141 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4201 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4261 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
     4321 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4381 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4441 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4501 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4561 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4621 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4681 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     4741 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     4801 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     4861 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     4921 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     4981 aaaaaaagcc catcgtggga gatgggaaaa gactacacac agcaattcgt tgtttcactc
     5041 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5101 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5161 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
     5221 atcaattagc ggtgattaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
     5281 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggtggcggg
     5341 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
     5401 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa cgccagagag gatcaggatc
     5461 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
     5521 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccggt ccagtagagc
     5581 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
     5641 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
     5701 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
     5761 gccgccagca gcacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
     5821 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
     5881 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
     5941 gtgccaatat aagggcggac tttcgccatg atcgccgtac cccccatatc cagcaagttg
     6001 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
     6061 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
     6121 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
     6181 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
     6241 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
     6301 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
     6361 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
     6421 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
     6481 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
     6541 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
     6601 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
     6661 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
     6721 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
     6781 cagatttccc tggcacccgg tgtcgttcaa ctggtgctta attttttgca agagctggag
     6841 caacaaggtt ttaccggcga tgcggcgaca agttatgccg atcgtctgac aatgtcgacc
     6901 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
     6961 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
     7021 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
     7081 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
     7141 gccggggtga taaaagacca actcaatcag tacctgaaac cgttcggcta cttttttgcg
     7201 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcatcc
     7261 ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt acgcgcggtg
     7321 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgagctggc ggaaacgctg
     7381 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
     7441 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
     7501 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
     7561 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcg cttgcctaaa
     7621 gtgcgccgtc tggtgaatgt caaatatgac tcttttgact ctgcgctgcg taacgcgccg
     7681 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
     7741 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
     7801 gagatgctcg gactgaacat tgtggaattt gctggtgatg atgaggcgct gattgacgag
     7861 cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
     7921 attggctggc aggtgtgccg cgagctggcg ggggttgaag gtatctatgc gatgcgcaaa
     7981 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
     8041 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
     8101 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
     8161 cgtccggcac tggatatgtg cgatccgcaa caagagattt tgatgaagca aatctctgat
     8221 gacgtggtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
     8281 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
     8341 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
     8401 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
     8461 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
     8521 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
     8581 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt gttggcggat
     8641 cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
     8701 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
     8761 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc gacccagtgc
     8821 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
     8881 tatttacgcc cgcttcgcga ccacctcgtt gctacggtcg agagctacgc accgctgatg
     8941 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
     9001 gaaaaacata tcggcatggt tgatttgcca ctgctgtcgg tcccctcgct acaacaacaa
     9061 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct caatgcagag
     9121 cagaaagcgc gcacggtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
     9181 gtggtggcgg attttgtccg tctggttgaa aaattaggtt tccagcctgt gttactgcca
     9241 ttttcgccaa atggcaaagc ccagcatatc aaaggctttc ttaatcgttt tgcgaagacg
     9301 gcgaaaaaga cggcggattt cctcaaccgt atggctaagc ttgggatgcc aatggtgggc
     9361 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
     9421 cgtggcgagt ttaacgtctt acttgcgaat gaatggctgg caagcgcact tgagtcacat
     9481 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaggttacc
     9541 gctttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
     9601 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
     9661 catgaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
     9721 cgtaaccgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg ggttgaaggc
     9781 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgga
     9841 aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa catggtgggg ttcctggata
     9901 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
     9961 caaagcagcc tttcgggttg ctgcatggag gagcatccgt ggtactggcc gaaagtatcg
    10021 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    10081 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    10141 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    10201 gctgttcgtc acgattgacg actgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    10261 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    10321 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    10381 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    10441 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    10501 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    10561 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    10621 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    10681 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    10741 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    10801 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    10861 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    10921 cctgttaacc cacacatcag gggctatgct tattaaaagc aggcaaaaag aatgttttac
    10981 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    11041 acgctgaaag cgatgaagtg aggtaaatag atggacatgc attcaggaac ctttaaccca
    11101 caggatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgcgag
    11161 ctggtggcaa agcagcctgg tatggtcggc gtgcgcttag gtgtgaagca aacgggctgc
    11221 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    11281 gaacacgacg gcgcgaagct gtttgtcccg ctgcaatcga tgccgtttat tgatggcacg
    11341 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    11401 cagaatgaat gtggctgtgg cgaaagcttt agggtatagg cggtactatg tctcgtaata
    11461 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    11521 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    11581 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    11641 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    11701 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    11761 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    11821 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    11881 ttttcgactc tgtttcggtt gccaccactt atcgcgaaaa actggcggag cagggaatta
    11941 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    12001 gcaccgtggt gccggggaat gacaacttct ttgcagcact taatgcggcg gtagcctctg
    12061 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    12121 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac aattctggtg gccgacgaag
    12181 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttgc
    12241 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    12301 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    12361 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    12421 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    12481 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    12541 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    12601 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    12661 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    12721 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    12781 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    12841 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    12901 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    12961 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    13021 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    13081 cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    13141 atttgcttgc actgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    13201 atccggtgga gattccaggc gtcagtaacc agtttttcct gcaaacggca cttaatgcag
    13261 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    13321 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    13381 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    13441 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    13501 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    13561 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtga
    13621 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    13681 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    13741 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    13801 catgaaaact ggaaatatac gccgctggaa gggctgacca atagtcagtt tgtcagcatt
    13861 gcgggagata tatccccaca gcagcgtgat tccttagcgt taacgttaga cgccgtgcgg
    13921 ctggtgtttg tcgatggacg ttacgtgccc gcgctgagcg atgcaattga aggcagcgga
    13981 tatgaagtga gcattaacga cgaccgtcag gggttacccg acgctattca ggcggaagtg
    14041 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    14101 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    14161 gtgaacactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    14221 atcgaacatt ttgtcagcct taatgatgct cgtcatttta ccggggcacg gttcactatc
    14281 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    14341 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    14401 ttcctgctgg gtggcgcagt gttacgacat aacaccagta cgcaactcaa tggcgaaaac
    14461 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    14521 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    14581 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    14641 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggataca
    14701 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    14761 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    14821 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    14881 cttaaacagc aggtgctggc ccgaatcggt caacggctgc caggaggtgc aagatgactt
    14941 tttccgtcga caaagtgcgg gccgactttc cggtgctttc gcgtgaggta aacggtttgc
    15001 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    15061 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    15121 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    15181 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    15241 atagctgggg caacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    15301 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    15361 tgatccctct caaccccgac ggtacgctgc aactggagac gctgcctacg ctgtttgatg
    15421 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    15481 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    15541 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    15601 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    15661 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    15721 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccgggg
    15781 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aatagcatag
    15841 ccgagtatga acagaatctg atgcattatg cgctatcaca gctggaatct gtaccggatc
    15901 tcactctcta tggccaacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    15961 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    16021 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    16081 ccatgtataa cacccatgaa gaagtggagc gtctggtgac cggcctgcaa cgtattcacc
    16141 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    16201 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    16261 agcgtctgcc agaattacgc gccgaagacg gaagcccaca aaatagcatt cagggctgcc
    16321 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    16381 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    16441 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    16501 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    16561 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    16621 ccggatggtc aatgtttaca ctgacctgca caggagtgtt ttggtttcag gtgaacataa
    16681 gcaccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    16741 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    16801 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    16861 gtgcaagaag gggataaaaa ccttcaggct attgcccgac gttttgatac tgcagcaatg
    16921 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacctggtac gacgataact
    16981 attccttcac aactgttatt acctgatgca ccgcgtcagg ggattatcgt taaccttgca
    17041 gagctgcgcc tttattatta tccgccggga gaaaatattg tgcaggttta tccaataggt
    17101 attggattgc aggggctgga aacgccggtg atggaaacgc gtgttgggca gaaaatccct
    17161 aacccaacct ggacgcctac ggcaggcatt cgtcagcgtt cgctggagcg tggcattaaa
    17221 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    17281 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgttggtttg
    17341 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    17401 gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    17461 aacgggatgc gttatgttga agtacatcga ccactatcag cagaagaaca gcaaaacgtt
    17521 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    17581 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt ttcggtgagc
    17641 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    17701 ccagagcaag ggaatatgtt acgcgcgacg cagtagtggt aaatagcaga caaaaaaatg
    17761 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    17821 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    17881 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    17941 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    18001 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    18061 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    18121 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    18181 aaaaaaattg agagaaaaca ggatgcttcc atcggattca tcttaaataa agtgagatta
    18241 tatagtgaaa tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaaagcg
    18301 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
    18361 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
    18421 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
    18481 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
    18541 agccgttttt tcgttggtgg tcagtgccag gatggtggca tccgggaagt atttacgtac
    18601 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
    18661 agcagtttca acggcaccac ggcataccgc ttcggtgatg cgcagtttac ggttgtcatt
    18721 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
    18781 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
    18841 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
    18901 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
    18961 tttttcgatc atcatcttct gggcgaagat aacttcttct accgggattt ctacacccag
    19021 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
    19081 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
    19141 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
    19201 tacgcctttt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
    19261 agcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcac cgttgttcag
    19321 cactttacag ataactttgt taccttcaat ggcggtaact tccataccga tcagaccatc
    19381 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
    19441 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgaccagc
    19501 tttcagagaa acatcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
    19561 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
    19621 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
    19681 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
    19741 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
    19801 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgcctcgctc
    19861 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
    19921 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
    19981 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
    20041 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
    20101 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
    20161 ataagtgaaa gttcatccgg tgtataaata tatcatcgct caattgctac agttaaaata
    20221 atttatcgcg ttatttgtct gattttgttt gatttttgtt taattggtta gattgatata
    20281 tgctgat
//
LOCUS       DADLCQ010000075        19743 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000075 DADLCQ010000000
VERSION     DADLCQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.075"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            264..1361
                     /gene="mltA"
                     /locus_tag="JQG95_002493"
     CDS             264..1361
                     /gene="mltA"
                     /locus_tag="JQG95_002493"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY5889012.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1600..2406
                     /gene="tcdA"
                     /locus_tag="JQG95_002494"
     CDS             1600..2406
                     /gene="tcdA"
                     /locus_tag="JQG95_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY5889013.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2457..2900)
                     /gene="csdE"
                     /locus_tag="JQG95_002495"
     CDS             complement(2457..2900)
                     /gene="csdE"
                     /locus_tag="JQG95_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY5889014.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2900..4105)
                     /gene="csdA"
                     /locus_tag="JQG95_002496"
     CDS             complement(2900..4105)
                     /gene="csdA"
                     /locus_tag="JQG95_002496"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY5889015.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4297..4524
                     /locus_tag="JQG95_002497"
     CDS             4297..4524
                     /locus_tag="JQG95_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY5889016.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4875..5792
                     /locus_tag="JQG95_002498"
     CDS             4875..5792
                     /locus_tag="JQG95_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY5889017.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5811..6206
                     /locus_tag="JQG95_002499"
     CDS             5811..6206
                     /locus_tag="JQG95_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY5889018.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6199..7299
                     /gene="rlmM"
                     /locus_tag="JQG95_002500"
     CDS             6199..7299
                     /gene="rlmM"
                     /locus_tag="JQG95_002500"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY5889019.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QTQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7343..8074)
                     /gene="fucR"
                     /locus_tag="JQG95_002501"
     CDS             complement(7343..8074)
                     /gene="fucR"
                     /locus_tag="JQG95_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY5889020.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8132..8554)
                     /gene="fucU"
                     /locus_tag="JQG95_002502"
     CDS             complement(8132..8554)
                     /gene="fucU"
                     /locus_tag="JQG95_002502"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY5889021.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPLLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8556..9975)
                     /gene="fucK"
                     /locus_tag="JQG95_002503"
                     /pseudo
     CDS             complement(8556..9975)
                     /gene="fucK"
                     /locus_tag="JQG95_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10084..11859)
                     /gene="fucI"
                     /locus_tag="JQG95_002504"
     CDS             complement(10084..11859)
                     /gene="fucI"
                     /locus_tag="JQG95_002504"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY5889022.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYDEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATKWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11892..13208)
                     /gene="fucP"
                     /locus_tag="JQG95_002505"
     CDS             complement(11892..13208)
                     /gene="fucP"
                     /locus_tag="JQG95_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY5889023.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAELFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13755..14402
                     /gene="fucA"
                     /locus_tag="JQG95_002506"
     CDS             13755..14402
                     /gene="fucA"
                     /locus_tag="JQG95_002506"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY5889024.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14430..15578
                     /gene="fucO"
                     /locus_tag="JQG95_002507"
     CDS             14430..15578
                     /gene="fucO"
                     /locus_tag="JQG95_002507"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY5889025.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15631..16476)
                     /gene="xni"
                     /locus_tag="JQG95_002508"
     CDS             complement(15631..16476)
                     /gene="xni"
                     /locus_tag="JQG95_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY5889026.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16497..17864)
                     /gene="sdaB"
                     /locus_tag="JQG95_002509"
     CDS             complement(16497..17864)
                     /gene="sdaB"
                     /locus_tag="JQG95_002509"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY5889027.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSLDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17922..19211)
                     /locus_tag="JQG95_002510"
     CDS             complement(17922..19211)
                     /locus_tag="JQG95_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY5889028.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4872 a         4837 c         5208 g         4826 t
ORIGIN      
        1 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgtccgt
       61 ggatgcgcac tatactccgc tcgcgttttc atgcaacctt ttttttaccc cgatcacgga
      121 tttagcatga ttttgaacaa aaacaaacca ctaaagccta ttttttgtgc aaaatttgcg
      181 tcagggagag gctgctcatt tcattaaacg cggcggtttg ttatcttcgt tgcgccttat
      241 tttttaacct gaagaagaga acaatgaaag gacgttgggt aaagtacctt cttatgggca
      301 cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac cgatcgcgga cagcaatata
      361 aagacgggaa atttacccag cctttctctc tggtgaacca gccagatgcc gttggcgcgc
      421 cgattaacgc cggtgatttt gccgagcaaa ttaaccatat ccgtaattcg tcaccgcgtc
      481 tgtatggcaa ccagagtaat gtttataacg cggtgcaaga gtggctgcgc gcaggcggtg
      541 atacccgcaa tatgcgccag ttcggcattg atgcttggca gatggaaggt gccgacaact
      601 atggtaacgt gcagtttaca ggctattaca cgccggtaat tcaggcgcgc catacccgcc
      661 agggcgagtt ccagtatcct atttaccgta tgccgccaaa acgtggtcgt ctgccgtctc
      721 gtgcggagat ctacgccggg gcattgggcg ataaatacat cctcgcttac agcaactctc
      781 tgatggataa cttcattatg gatgtgcagg gtagtgggta tatcgacttt ggtgatggca
      841 gtccgcttaa ctttttcagc tatgcaggga aaaacggtca tgcctatcgc agcattggta
      901 aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat gtcgatgcag gcgattcgtc
      961 attggggcga aacacacagt gaagccgagg ttcgcgagct gctggaacag aacccgtctt
     1021 tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg ggcaagtgcg gtgccgctgg
     1081 ttggccgtgc ttctgttgcc tccgatcgtt caattattcc gccaggtact accttgctgg
     1141 cagaagtgcc gctgctggat aataacggca aatttaacgg tcagtacgaa ttgcgtctga
     1201 tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca cttcgatatc tatcaaggga
     1261 tcgggccgga agccggacac cgcgcaggtt ggtacaacca ctatggacgg gtttgggtgc
     1321 tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg atgtgatatt ctgtatgccg
     1381 tttttacgga tatgacaggg tgaggggtaa cctcacatcc tgattattga ctaagtgcgc
     1441 gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcat gcaaatccaa
     1501 aatattgcag gagttatgta ggcctgataa gcgtagcgca tcaggcattt atgcgtttag
     1561 ggtgagggat aacctcgccc tttttaattc tgaggtgcta tgtctgtggt aattagtgat
     1621 gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg gtgaaaaagc gttgcaactg
     1681 tttgctgacg cgcatatttg tgtggttggt atcggtggtg tcggttcctg ggcggcggaa
     1741 gcgctggcgc gcacggggat tggcgcaatc acgcttatcg atatggatga tgtgtgcgtc
     1801 accaatacca atcggcaaat tcacgctctg cgcgataacg tcgggctggc aaaagcggaa
     1861 gtcatggcgg agcgcattcg ccagattaac ccggagtgcc gtgtaacggt ggtggatgat
     1921 ttcgtgacgc cggataacgt agcgcaatat atgagtgccg gttattcgta cgtgattgat
     1981 gccattgata gtgtacggcc caaagcggcg ctgattgctt attgtcggcg caataaaatc
     2041 ccgctggtca cgaccggtgg cgcgggtggg cagattgacc cgacgcagat tcaggttacc
     2101 gatctggcga aaacgattca ggatccgctg gcggcgaagt tgcgcgagcg cctgaaaagc
     2161 gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg tggattgcgt gttttctact
     2221 gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg cgatgaaagc cacggcagaa
     2281 gggccgaagc ggatggattg tgcatctgga tttggcgcgg caacgatggt gaccgccacc
     2341 tttgggtttg ttgcggtttc tcatgcgctg aagaagatga tggcgaaagc ggcgcgtcag
     2401 ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc cgacccggtt ctgtgcttaa
     2461 acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat ttaacccctg gctgcgtgag
     2521 gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg ccagcggcga ctgtgcctgt
     2581 aattcggcgg cggttttccc ctcaacggca gtcaacaaca ccgccaacag gccgcgcaca
     2641 atgcgccctt cgctgttgcc aaagaagtgc attttgccgt tttcagccac ggtatatccc
     2701 agccagacgc ggttttcgca tccggcaatc tctttagcct gcgcttttaa ctcgtctggc
     2761 aaagccggaa gctgtttccc cagcatgatc aactggcgat atttatcttc ccattgcgac
     2821 aacggtgcga aggtattgcg taacgtttct gcggttacgg ttgtgccgaa cggatgtccg
     2881 gcgaattgcg ggtttgtcat taatccacca ataattccag cgcgcggtca acggcattca
     2941 ccagcgcatc cacatcactc tttgtattat atggcgcaaa tgaggcgcgc agtgtgccgg
     3001 ttacgcctaa ttctgccagt agcggctgag cgcaatgttg cccggcccgc aaggcaatac
     3061 cgtactccgc cagtagcgtc accatatcgc tgtgatgaac gccagcaaaa tcaaaggcca
     3121 gcaggctgga atcctggcag cggaatgaac gaaagcctgg acgtttcgcc agcgcttctt
     3181 ctgcaagcgt tgctaagcta cggctccagc tttcggcctg gttgatatcg taatctgcca
     3241 gccattccag cgccgcgctt aatcctatga cgccagcgac atttggcgtt cctgcttcca
     3301 gtttccacgg cgcagattga gtggtgaaac cgtcaaaact cacttcgtga atcattttgc
     3361 cgccacccag ccagggcgac atcgcttcca gcagttctga tttgccatac agcacgccga
     3421 tacccgtcgg gccatacagt ttgtgaccag agaaagcata gaaatcgata tccagttgct
     3481 gaacatccgc ggggaaatgc accgccccct gagcaccatc aaccatcacc accatcccgg
     3541 ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca accgccagtc acgttcgaca
     3601 tctgacccaa cgccagaatc cgactacggg gagtaatcag ctctggcaac aaatcgacat
     3661 ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt ggctctagtt tgttgggcga
     3721 ccatcagcca ggggacgagg ttagcgtggt gttctgccac gctgacaata atctcatcgc
     3781 ccggttgcag acgcggacgc gcatagcatt gtgccaccat gttgatggat tcagtggtgc
     3841 cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag taattgcgcc actttctctc
     3901 gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc aaactggctg cgatggacgt
     3961 ttccggcgct caggctgtaa aactgtcgag tggcttcaac cacggcttca ggtttaagcg
     4021 cggtcgcggc gctgtcgaga tagacgcccg catcctgtag tgcgggaaac tgggcgcgaa
     4081 actgcgcggg attaaaaacg ttcatggtac tcctcggctt gatgcaccga tcgtgacgca
     4141 aaattcactt tgatacaagg agagtgctaa ccatcagaat tatctggata tcctggcgaa
     4201 gccgcgtaag taacatatgc tgaatagtgt taggcgttaa ttttagcctg ttaatcaata
     4261 ctattaagat ttattaatcg ttttaaggag aagatgatga aaaagactgc cgcaattatt
     4321 tctgcctgta tgctgacttt tgccctgagc gcctgttccg gttcgaacta tgtgatgcac
     4381 accaatgacg gacgtaccat cgtctctgac ggcaaaccac agactgataa cgatatcggt
     4441 atgatttcgt ataaagacgc taatggcaac aaacagcaga tcaaccgtac tgacgtgaaa
     4501 gagatggtcg aactggaaca gtaattcgcg atcgcaaggt aaaaaaaagc accgcaatta
     4561 ggcggtgcta cattaatcac tatggacaga cagggtaaat gtacaggaag tgaaaaaagg
     4621 tagctttgct accatggtct gaatcgcaga ccaattgcaa acacaacaac acaacatcac
     4681 aaccgtaagc caaaagttca ccagaatacg cattccgata aaacttttcg ttccggctca
     4741 ggaagtgccg ccactatagg tatttgctgg tagaagctca acggacaatt tataatggct
     4801 cagattaaaa aaactaatag gttacacagt gtgatctaat tgttaaattc atttaacatc
     4861 aaagtttaaa agccatgtct aaacgattac caccgctaaa tgccttacga gtttttgatg
     4921 ccgcagcacg ccatttaagt tttactcgcg cagcagaaga gctttttgtg acccaggccg
     4981 cagtaagtca tcaaatcaag tctcttgagg attttttggg gctaaaactg ttccgccgcc
     5041 gtaatcgttc actcctgctg accgaggaag ggcaaagcta tttcctcgat atcaaagaga
     5101 tattttcgca attaaccgaa gcgacgcgta aactccaggc ccgtagcgcc aagggggcgt
     5161 tgacggtcag tttactcccc agtttcgcca ttcattggtt ggttccgcga ctttccagct
     5221 ttaattcagc ttatccggga attgacgttc gaatccaggc ggttgatcgt caggaagata
     5281 agctggcgga tgatgttgat gtggcgatat tttatggtcg gggcaactgg ccggggctac
     5341 gggtggaaaa actgtacgcc gaatatttat tgccggtgtg ttcgccgcta ctgctgacag
     5401 gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca tacgttatta catgatgcgt
     5461 cacgccgtga ctggcagaca tatacccgac agttggggtt taatcatatc aacgttcagc
     5521 aagggccaat ttttagtcat agcgccatgg tgctgcaagc ggctattcac gggcagggag
     5581 tggcgctggc aaataacgtg atggcgcaat ctgaaatcga ggccggacgt cttgtttgcc
     5641 cgtttaatga tgttctggtc agtaaaaatg ctttttatct ggtttgtcat gacagccagg
     5701 cagaactggg taaaatagcc gcctttcgcc aatggatcct ggcgaaagcc gctgctgaac
     5761 aagaaaaatt ccgctttcgt tatgaacaat aatttacgta gggtacgacc atgaccagcc
     5821 gttttatgct gattttcgcc gctattagcg gcttcatttt tgtggcactg ggcgcttttg
     5881 gtgcgcatgt gttaagtaaa accatggggg ccgttgagat gggctggatc caaaccggtc
     5941 tcgaatacca ggcgtttcat acgctggcga tcttaggtct ggcggtggca atgcagcgtc
     6001 gcatcagtat ctggttttac tggagtagcg ttttcctcgc gttaggcacg gtgctgttca
     6061 gcggcagcct ttattgcctg gcgctgtccc atctgcgttt gtgggcgttt gtcacaccgg
     6121 ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt agttggtgct atccgtttaa
     6181 agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt gccgtccggg ctttgaaaaa
     6241 gagtgcgccg cagaaattac cgataaagcc ggccagcggg aaattttcgg ttttgcccgc
     6301 gtgaaagaga atgcgggtta tgtcatttat gaatgttatc aacctgatga tggcgataag
     6361 ttaatccgtg agctgccgtt cagttcatta atttttgccc gccagtggtt tgtggtgggg
     6421 gaactcctgc agcatttgcc gccagaagat cgtattaccc ccattgtcgg catgttacag
     6481 ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg ccgataccaa cgaaagcaaa
     6541 gagttgctga aattctgccg taagttcact gttccgctac gcgctgcctt gcgcgatgct
     6601 ggggtgctgg cgaactatga aacgccgaag cgtccggttg tgcatgtatt ctttattgca
     6661 ccaggatgct gctataccgg ttactcatac agcaacaata attcgccgtt ctatatgggc
     6721 attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt ccacgctcaa actggaagag
     6781 gcatttcatg tgtttattcc tgcggatgag tgggatgaac gcctggcgaa cgggatgtgg
     6841 gcggtggatt taggcgcttg ccctggcggc tggacctacc aactggtgaa gcgcaacatg
     6901 tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc tgatggatac cgggcaggtg
     6961 acgtggctgc gggaagacgg tttcaaattc cgtccgacac gcagcaatat ctcctggatg
     7021 gtatgcgata tggttgaaaa accggctaaa gttgcggcat tgatggcgca gtggctggtt
     7081 aatggctggt gccgtgaaac cattttcaat ctcaaactgc cgatgaaaaa acgctacgaa
     7141 gaagtatcac acaatctggc gtatattcag gcacagcttg atgaacatgg cataaatgct
     7201 cagactcagg cgcggcagtt gtatcacgat cgcgaagaag tgacggtgca cgtccgccgc
     7261 atctgggctg cggtgggtgg tcgtcgcgac gagcgataac aggcaaggcc ggggcgtcgt
     7321 ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga agttgcaacc tggcgctcat
     7381 cagaaataat gtgcgttacc tcatccagat gcccgatgcg ggcttcccct gaacgattaa
     7441 atttactttt atcaatcaat aacaacgatt gcgcggcacg ttttaatagc atcgatttgt
     7501 aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct gctatcgatc ccttcacaag
     7561 aaaaaataaa cagatcgatt tccagcgatt taagttggga aatcagcgag ggattgacgt
     7621 aacagccata tttgcgctca agtgtgccgc cggaactgat cagttgaatg cgttcgcgtt
     7681 taccgagttc atggcaaatc ggatggctat tggtgaagac ctgaatgttg atgtcaggca
     7741 actggcgtgc cagataccag caagttgaac tggcgtctaa ggctatcacc atcccttctt
     7801 caatccacgc gagcgcctcg cgcgcgatat ctgctttatg cgcataatgg cttttcagcc
     7861 tgatgtgaaa gggatcgcca ctgtcttgat tttgacggtg gatatattta gcgcgtccat
     7921 gattgcgcag aattttaccc tgcgtctgta attcattgag atcgcgacga atggtttctt
     7981 tactgacctt tagctgttca gagagagctt ccgtggtcag gctggtatgg ttcagcagca
     8041 ggtcgactat cgcttgctgg cgtgccgctt tcatattttt caccccatca ttcgataacc
     8101 gcccgcgtca tgcgggcgta ccggtatgag attacggtgt tacccctttt tttaaaagaa
     8161 tattcccgta cttcgctcgt tcgcctgtga taacgatcgc aaaggctttt tgcgcccgtt
     8221 cataaaacgc aaaacgattg atgcggatga tgtcaggaca cggggcttgt agtgaaagcg
     8281 cattacggta acgtcgttct acttcaggat cgagagtgtc accctctacc gccgccatca
     8341 tcaccagcag cggggcataa ctgtccagct caaataacgg gataatcgcc tggagcaagt
     8401 cgctcaccaa caggccatca gcgcggatca cctgcggttc catcgaatgg gcgggaaagt
     8461 gagcatcgga aaaaataatt tcatctccat gtcccatctc tgccagcact ttcaatagtt
     8521 cgggagaaat taacggcgaa attgttttca gcatttcaca cttcctctat aaattcaggt
     8581 tcagtttgcg ggtagaaata acggtactga taatgaatct gtgcacgggc ttcttccggg
     8641 ctgttaaatt cccctacgcc ataccaaccg aacagcgcag ctcctgcgac ggtcgtttcg
     8701 gcatcgtcga gaacttttac cggaatatca agcatattgg ctttaatctg attccacaat
     8761 gtgttgcgac ttcctccacc gactaacaat aattcatagg ctttaaagtg cccgattttt
     8821 tccagcatct gtagattgcg ctgtaattgc gcagttaacc cttccagcgc cgcgcgatag
     8881 aaatgccccc gcgtggtatt aagcgtcact ccttgccagc cagcgttctg acacgacaat
     8941 aaatcacact gcattttcac accatccgcg ccaggtgcga tcagacgagc ttcttcaatc
     9001 aacatttgcc agggtgtttc agccgtccag aacagttttc tcacccattc cagcacgccg
     9061 gatgccagcc attgcatacc tgggttatac aaccatgcct ggctatctag ttcgcaggtg
     9121 gaaccggcgt actgacttaa cagcgaagta tcaacctagg cgctgcgaac cattaaaatt
     9181 tcccatgtac cggaggagag cacgggttca ttttgttcgg caccagcgcc aaaaagggcg
     9241 aactgggtat cgtgacctgc ggaaatcacc ggtatgccga cgggtaagcc gagcattgct
     9301 gcggcgttgt tctgtagcgt accaatctgt tcacccgctt ccaccagacg agggaagagt
     9361 cggcgtggaa taccggtggc ttgtaaaatt tgcggactga aatcgcgttg ctggatatcc
     9421 agcatctggc tggttccggc catcgtgata tcagtagtga attcgctggt taaacggtgg
     9481 ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt ccagcagttg tggatgattt
     9541 tctttcaacc acaccaactt atataacgta ttgaaactaa aggctccgac gccagaaata
     9601 gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt ccataaccgc tgctgttcgc
     9661 ggacatttcc agctaatgat cggatagagc agattgcctt gcttatctac cagagcgcca
     9721 tccacactaa aggtggtgac ggcgataccg cggatgtggc attcagtcag ttcactattg
     9781 atttgccgac agcaatcagc aaagcgttgc aaaatggcag tctaaagacc actggtgcca
     9841 ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt
     9901 tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat
     9961 aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgttt atccggctaa
    10021 ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggtgtaat
    10081 gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg cggtaatcct ggccttcaat
    10141 atccatgccg tgcgcagccc aggcagaagg acgatacact ttggtctctt caacgttgtg
    10201 catacatacc gggatacgca gcatggaggc aagggtgata aagtctgcgc caacgtggcc
    10261 gatggtcaga actccgtggt tggcacccca gttcgccatt accgaataca catccgtaaa
    10321 cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg
    10381 tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg
    10441 cagtaccggt cccaggcctt tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc
    10501 ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca
    10561 ccatttggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt
    10621 catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc
    10681 tgagttgatc aaatggatga tgccgtgttc tgccagtccg tccagtttat gccccgttac
    10741 acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatacct gagcggtgcc
    10801 ggtgagctgg tgacccatta gcattgccac gccgttaaga ctgtcgtttt cggtcgccac
    10861 gacaaagggt tcgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcggcggt
    10921 gtcaccattg ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc
    10981 gttgtagcca agtgattctt ccacgcgacc aatatcggcc agtttgctgt tgccttgcat
    11041 catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc
    11101 ggcattgcgt tgatactgtt tgttattttc atcttcgtca tagcggaagt ttttatcagc
    11161 ccaggccagt gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg
    11221 cagttcggtc atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg
    11281 atcaacaata gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc
    11341 tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc
    11401 ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg
    11461 gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc cggggcgttc
    11521 agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact
    11581 gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct acattctggc tgctgaattt
    11641 ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc
    11701 gacggcagct ccgcaggcat ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc
    11761 catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac
    11821 cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc
    11881 ggaaaatatg ttcagttagt tgccgtttga gaacggaaac gggcaaagat aaagatgacc
    11941 gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca
    12001 aaacccatga ccggagtgac aataccgccg ccaataatgg tcataacgat gaaggacgaa
    12061 ccgtatttgg tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga
    12121 atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc gccagcgaag
    12181 gctgagatca ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt
    12241 gcgaagcgac tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt
    12301 ccggttaaat agttagcggc aaagcctgca gtcatacctg gaatttcttc tacagcgtag
    12361 cgaatcaaat agctccagca ggccgtttgt gcgccgacat agcagaattg cgctaatacc
    12421 gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg atgcggagaa cgatccttgt
    12481 ttggcgtcac tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg
    12541 gcgaccagta acacgatagc cacgatgatc atataaggtg tctgtaccga taataccagg
    12601 ctgtgtttat acgcactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga
    12661 tgtggcacgt tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaac
    12721 gagttaaatg tttgcgcaag atttaagcgg aagtgactac tgctttccgg ccctaatacc
    12781 gtaacaaaag ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg
    12841 ccaactaaaa ataaggtgta gttcattatt tctgcagcgg gccagaataa ttcagcgccc
    12901 aaggcatata aaaataatcc ggtaataatc cctgctttat aactgagttt tttcatcaat
    12961 atcccagcag ggattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca
    13021 gcctggaaat ttgtcagcgt aaaagcctgc tggaattgag gtaataaaat gtcgttaagg
    13081 ttattggcta ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa
    13141 cttctgcttt gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg
    13201 tttcccatag catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa
    13261 agtgcaaaac cagcgagttc gggcttcata tcggtgaata tttgcctgtc tgaaatgaac
    13321 tcatggattc attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag
    13381 ctggaaggtg actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc
    13441 agaaaataaa tcataaaaaa cacaaaccag atgaccgtat taattgtttg tgccgaaaac
    13501 gggcatttct ggcgagggga aatggaaaaa tgaccgtttt gataaatatt tttaagagcg
    13561 gtcattttta acggggttat aaaaagagaa cttgcagtta tttactgtga ttagttgagc
    13621 taggtcacaa aatataagct tgtgaatttc tttcttgtta atcaggaaga agatggtggg
    13681 taattaaacg gctaattcaa tagtgtgaaa ggaacaacat tattgccctg ttttgaatca
    13741 gagagaggta gctaatggaa cgaaataaac ttgctcgtca gattattgac acttgcctgg
    13801 aaatgacccg cctgggactg aaccagggga cagcggggaa cgtcagtgta cgttatcagg
    13861 atgggatgct gattacgcct acaggcattc catatgaaaa actgacggag tcgcatattg
    13921 tctttattga tggcaacggt aaacatgagg aaggaaagct cccctcaagc gaatggcgtt
    13981 tccatatggc agcctatcaa agcagaccgg atgccaacgc ggttgttcac aatcatgccg
    14041 ttcattgcac ggcagtttcc attcttaacc gatcgatccc cgctattcac tacatgattg
    14101 cggcggctgg cggtaattct attccttgcg cgccttatgc gacctttgga acacgcgaac
    14161 tttctgaaca tgttgcgctg gctctcaaaa atcgtaaggc aactttgtta caacatcatg
    14221 ggcttatcgc ttgtgaggtg aatctggaaa aagcgttatg gctggcgcat gaagttgaag
    14281 tgctggcgca actttacctg acgaccctgg cgattacgga cccggtgcca gtgctgagcg
    14341 atgaagagat tgccgtagtg ctggagaaat tcaaaaccta tgggttacgg attgaagagt
    14401 aatttcgtaa agcaacaagg agaaggatga tggctaacag aatgattctg aacgaaacgg
    14461 catggtttgg tcggggtgct gttggggctt taaccgatga ggtgaaacgc cgtggttatc
    14521 agaaggcgct gatcgtcacc gataaaacgc tggtgcaatg cggcgtggtg gcgaaagtga
    14581 ccgataagat ggatgctgca gggctggcat gggcgattta cgacggcgta gtgcccaacc
    14641 caacaattac tgtcgtcaaa gaagggctcg gtgtattcca gaatagcggc gcggattacc
    14701 tgatcgctat tggtggtggt tctccacagg atacttgtaa agcaattggc attatcagca
    14761 acaacccgga gtttgccgat gtgcgtagcc tggaagggct ttccccgacc aataaaccca
    14821 gtgtaccgat tctggcaatc cccaccacag caggtactgc ggcagaagtg accattaact
    14881 acgtgatcac tgacgaagaa aaacggcgca agtttgtttg cgttgatccg catgatatcc
    14941 cgcaggtggc gtttattgat gctgacatga tggatggtat gcctccggcg ctgaaagccg
    15001 cgacgggtgt tgatgcgctc actcatgcta ttgaggggta tattacccgt ggcgcgtggg
    15061 cactaaccga tgcactgcac attaaagcga ttgaaatcat tgctggggcg ctgcgaggat
    15121 cggttgctgg tgataaggat gccggagaag aaatggcgct cgggcagtat gttgcgggta
    15181 tgggcttctc gaatgttggg ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt
    15241 tttataacac tccacacggt gttgcgaacg ccatcctgtt accgcatgtc atgcgctata
    15301 acgctgactt taccggtgag aagtaccgcg atatcgcgcg cgttatgggc gtgaaagtgg
    15361 aaggtatgag cctggaaggg gcgcgtaatg ccgctgttga agcggtgttt gccctcaacc
    15421 gtgatgtcgg tattccgcca catttgcgtg atgttggtgt acgcaaggaa gacatttcgg
    15481 cactggctca ggcggcactg gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc
    15541 ttgaggatat tgtagagctt taccataccg cctggtaaat gcgctgatgt gataatgccg
    15601 gatacgattt gcggcgtatc cggctcgccg ttaccgtacc aaccgcaatt gctgtaaatt
    15661 gccgtcgata tgcaaatcgg tttgtaagcg ggcaatatcg cggcacagaa acgccatctc
    15721 tttatgggtt tctaattttt tgcgccactt ttcggcaacc gcatccagat tctcatatat
    15781 cccttccaga ctctgaaact cgaccagcag ctgcgtggcg ctttttggtc cgattcccgc
    15841 aacacccggt acctttgaac tgctgatccc cgcaagtccc cagtaatcgg gcaactgctg
    15901 cggttgaacg ccaaattctt tatcgataaa tggcgcatcc agccaacgtt tctggaagta
    15961 atcacgaata cgtaatgtcg gtgaaagtaa ctgacagtag cctttatctg tcgaaacaat
    16021 cgttgcctga tgcccggcct gtgtcacttt gaccgccagt gtggcggcta agtcatcggc
    16081 ttcgttgccg ctggctgacc agcacgggac gccgcgttgc tcaaaggcgg cgcgtaatgc
    16141 aggcatctcg tcgtgcaact cttccggcat tggcggtcga tccgctttgt aatctggtaa
    16201 acgctgatga cgccagccgc tactgcggtt ttcatcatca aaaacggcga ccgcgtgggt
    16261 tggctggctg tgcataatga gctgatcgag cgcatgctgg caggtttcga cacagggcga
    16321 accctgaacg gcatgaatgc gacggataag attcagtgca tcgacaataa gcaaatgaac
    16381 agccacgata acccccttaa agatcaggcg ataagggtaa cattcgatgc cggaacaggc
    16441 tatggctggt tgagaaaaca ggaagaggct tcgcaaaacg aggcctctgg gagagattaa
    16501 tcgcaggcaa cgatcttcat cgccaggccg ccgcgagagg tttcgcggta cttggcgttc
    16561 atgtctttac ctgtttcgta catggtttcg ataactttat cgaggcagac gcgcggttcg
    16621 ctggtacggc gcagtgccat acgcgcggcg ttcaccgctt tcactgctgc aatggcgtta
    16681 cgctcaatgc acggtacctg aacctgcccc gcgaccgggt cacaagtcag accgaggttg
    16741 tgctccatcg cgatttccgc tgcgatgcaa acctgcgccg ggcttgcgcc taacagttct
    16801 gccagaccag ctgccgccat agagcacgcc acgccaactt caccctggca acccacttcg
    16861 gcaccagaaa tcgacgcgtt catcttataa agagaaccaa tggcgctggc taccagcagg
    16921 taacgagcca gtgagttagc gttcacttcg cggataaact tatcgtagta cgccagcaca
    16981 gccgggataa tcccgcacgc accgttagtc ggcgcagtca ccacgcgacc gccagcagcg
    17041 ttctcttcgt tcactgccag tgcaaacatg ttgatccagt caacaaccgc catcgggtca
    17101 gtggtggttt tatccaggct gaccagcatc cggcgtagtg ccgcagcacg gcgtggaacg
    17161 cgcagtttgc caggcaacac gccttcggtg gaaataccgc gctcaatacc gccacgcatg
    17221 acttcccaga cgttcgccag gtgctgttcc agctcttctt tactgtgcag cgccagctcg
    17281 tttttcatca tcaggccaga gagtgacagc ccggtttcct gacaatgttt ttgcagatcg
    17341 gctgctgaac tgtacggata aggaacttca accggtgcgc tatcctgctg gccaaaatgc
    17401 tcttcatcaa cgataaagcc accgccaata gagtagtaag tctggctgta aacgacttta
    17461 tcgcccgcca gcgcggtaat gcgcataccg ttttcatgca gagaaaggtt atctgcatgg
    17521 aagttcatgc actgatcaac cgggaactcc acttcatgct gaccgtttgc cagcatcagg
    17581 cgaccatgag tattcacatc ctgaataaaa ccggggatgg aatcgatatc cacggtatcc
    17641 ggcaggttac ccgccaggcc cataataatg gcgatatctg tgtggtggcc tttaccagtc
    17701 agagagagcg agccgtacac gtcaaccacc acgcgggtca cgtctttaag caggttacgg
    17761 gcaatcagat cgtcggtaaa ttgtttaccc gctttcattg gtccaacggt atgagaactg
    17821 gaagggccaa tgccgatttt gaaaatatcg aatacgctaa tcataggaaa tacatcgcgt
    17881 taaaacggag gaagcgccgc ccgaaagcgg cgcgaaagga cttagctgaa cagagagtag
    17941 aagattgcgg agattgcaat cagacccatc acgacaacga atacgttgct gatgtgaccg
    18001 ctgtacttac gcattgccgg tactttctga attgcgtaca tcggcatcag gaacaggatc
    18061 atcgcgatga ttgggccgcc cagggtttca atcataccca ggatgctcgg gttcagggtg
    18121 gcaacaatcc aggtcgttac cagcatgaac agcgcagtga tacggttcag cttgttgatt
    18181 tcgatagact tacctttacc acgcagagat ttaatcacca taccgttgaa gccttcacgt
    18241 gcgcccaggt agtggccgag gaaggatttg gtgatagcga taatcgcgat aatcggagcc
    18301 atccacgcga taaccggtgc gttaaagtgg ttagccaggt aagacagaat cgagatgttc
    18361 tgctctttag ccgcagccag gtctgccgga gtcaggctca gtacgcagct gaagacgaag
    18421 aacattacgg tcagcaccat catgatgtgt gcgaatgcca ggatcttcga gcatttctgt
    18481 tctgccatat cgccgtactc ttcacgcttc gcaacggcga aagaagagat gatcggagag
    18541 tggttgaacg agaacaccat taccggaatt gccagccaca gggtcatcca cagaccgttt
    18601 ccggttgcag atgcagtgtc cagagacagc gtttccagtg ctgcgccgtt ccactgcggg
    18661 atcaggtaca gagccagcag catcagtacg ccaacaaacg ggaataccag aatactcatc
    18721 gctttaacga tcatctgctc accaaagcga acgatggtca tcatacccac gatcaggatc
    18781 agcgacagaa tcgcacgcgg cggtggcgtc atacccagct ggtgagacat gaagctttca
    18841 acggtattgg tgattgccac gctataaacc agcaggatcg ggtagatagc gaagaagtag
    18901 agcagggtaa tcagtttacc tgcgccaata ccaaagtgtt cttctacaac ctcggtgatg
    18961 tcttcgcccg ggtttttacc agacagtacg aagcgagtca ggccgcggtg agcaaaaaac
    19021 gtcatcggga acgcaaggat agccatgatg atcagcggga tcataccgcc aacaccggcg
    19081 ttgattggca ggaacagcac gcccgcgccg attgccgtgc cgtaaaggcc cagcatccac
    19141 atggtgtctg tcttgcgcca ggcactacga gagtctttcg acgcaatcgt gctggtttga
    19201 gtcgtttcca tctatttctc ctggaggaat gcaaataatt cagcttttag ccagttcagc
    19261 cagggaaggt gcctgaccgt aaaaatgaaa tctgtctatc ggcgatggct taatcattta
    19321 ccgccgtaat aatttgaggg cggaaagata cacacaagtt atgtatctat cagtgatctt
    19381 gatcccaaat acaataatct gcttggaaga tagattatcc caacgcttta ctctgtaaat
    19441 caaaagtaaa agagaattta agggcaatat ttgcccatta aacgacatct gacttcttcc
    19501 ggggcgcgca atatatgtag gttgaactga aaaaatcaat acttttagtt aatcatggcg
    19561 ataatttctt tttcatagcg ataaaatacg gtttatgaaa atagggaaac gtttgtgttg
    19621 caacataaat tgtatgacat attaactgtg atgatatttg tcgcgtgagg gtggtaagaa
    19681 acattcatat aacaaatgaa atgagttgat gagtaagtga ccaaaaacga gaaaacccgg
    19741 caa
//
LOCUS       DADLCQ010000076        19728 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000076 DADLCQ010000000
VERSION     DADLCQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.076"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..766
                     /locus_tag="JQG95_002511"
     CDS             <1..766
                     /locus_tag="JQG95_002511"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY5889029.1"
                     /translation="KSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQS
                     VTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQA
                     GAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQ
                     PITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARD
                     PNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA"
     gene            complement(814..1503)
                     /gene="ung"
                     /locus_tag="JQG95_002512"
     CDS             complement(814..1503)
                     /gene="ung"
                     /locus_tag="JQG95_002512"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY5889030.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            1808..2191
                     /gene="grcA"
                     /locus_tag="JQG95_002513"
     CDS             1808..2191
                     /gene="grcA"
                     /locus_tag="JQG95_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY5889031.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(2247..2834)
                     /gene="eamB"
                     /locus_tag="JQG95_002514"
     CDS             complement(2247..2834)
                     /gene="eamB"
                     /locus_tag="JQG95_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY5889032.1"
                     /translation="MTPILLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            2937..3818
                     /locus_tag="JQG95_002515"
     CDS             2937..3818
                     /locus_tag="JQG95_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5889033.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(3853..5187)
                     /gene="srmB"
                     /locus_tag="JQG95_002516"
     CDS             complement(3853..5187)
                     /gene="srmB"
                     /locus_tag="JQG95_002516"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY5889034.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            5319..6056
                     /gene="trmN"
                     /locus_tag="JQG95_002517"
     CDS             5319..6056
                     /gene="trmN"
                     /locus_tag="JQG95_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY5889035.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(6041..7663)
                     /gene="nadB"
                     /locus_tag="JQG95_002518"
                     /pseudo
     CDS             complement(6041..7663)
                     /gene="nadB"
                     /locus_tag="JQG95_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            7919..8074
                     /gene="rseD"
                     /locus_tag="JQG95_002519"
     CDS             7919..8074
                     /gene="rseD"
                     /locus_tag="JQG95_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY5889036.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            8071..8646
                     /gene="rpoE"
                     /locus_tag="JQG95_002520"
     CDS             8071..8646
                     /gene="rpoE"
                     /locus_tag="JQG95_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY5889037.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            8679..9329
                     /gene="rseA"
                     /locus_tag="JQG95_002521"
     CDS             8679..9329
                     /gene="rseA"
                     /locus_tag="JQG95_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY5889038.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            9329..10285
                     /gene="rseB"
                     /locus_tag="JQG95_002522"
     CDS             9329..10285
                     /gene="rseB"
                     /locus_tag="JQG95_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY5889039.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            10282..10761
                     /gene="rseC"
                     /locus_tag="JQG95_002523"
     CDS             10282..10761
                     /gene="rseC"
                     /locus_tag="JQG95_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY5889040.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            10959..12758
                     /gene="lepA"
                     /locus_tag="JQG95_002524"
     CDS             10959..12758
                     /gene="lepA"
                     /locus_tag="JQG95_002524"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY5889041.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            12774..13748
                     /gene="lepB"
                     /locus_tag="JQG95_002525"
     CDS             12774..13748
                     /gene="lepB"
                     /locus_tag="JQG95_002525"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY5889042.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            14020..14700
                     /gene="rnc"
                     /locus_tag="JQG95_002526"
     CDS             14020..14700
                     /gene="rnc"
                     /locus_tag="JQG95_002526"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY5889043.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            14697..15602
                     /gene="era"
                     /locus_tag="JQG95_002527"
     CDS             14697..15602
                     /gene="era"
                     /locus_tag="JQG95_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY5889044.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            15614..16342
                     /gene="recO"
                     /locus_tag="JQG95_002528"
     CDS             15614..16342
                     /gene="recO"
                     /locus_tag="JQG95_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY5889045.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            16354..17085
                     /gene="pdxJ"
                     /locus_tag="JQG95_002529"
     CDS             16354..17085
                     /gene="pdxJ"
                     /locus_tag="JQG95_002529"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY5889046.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            17085..17465
                     /locus_tag="JQG95_002530"
     CDS             17085..17465
                     /locus_tag="JQG95_002530"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY5889047.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            17885..17965
                     /gene="shoB"
                     /locus_tag="JQG95_002531"
     CDS             17885..17965
                     /gene="shoB"
                     /locus_tag="JQG95_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY5889048.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(18159..18419)
                     /gene="yfhL"
                     /locus_tag="JQG95_002532"
     CDS             complement(18159..18419)
                     /gene="yfhL"
                     /locus_tag="JQG95_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY5889049.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            18634..19728
                     /locus_tag="JQG95_002533"
                     /pseudo
     CDS             18634..19728
                     /locus_tag="JQG95_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         4908 a         4854 c         5209 g         4757 t
ORIGIN      
        1 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
       61 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg
      121 gtttatccag agtgtaacgc cgcgttttaa agaagccttg cgctggatgg cagcaaaccg
      181 caaagcgtac catgtggtgg atgaagcgga attgacaaaa gcgtcaggta cggaacatca
      241 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaat
      301 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
      361 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
      421 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agcacgttca
      481 gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
      541 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa
      601 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
      661 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
      721 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
      781 ggcaactctg ccatccggca tttccccgca aatttactca cactctgccg gtaatactgg
      841 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
      901 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
      961 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     1021 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     1081 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     1141 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     1201 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     1261 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     1321 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     1381 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     1441 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     1501 catcttgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     1561 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     1621 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat caatgtgtgg
     1681 tttttgttga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     1741 acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt tgccggggag
     1801 gcatcacatg attacaggta tccagattac taaagccgct aacgccgatc tgctgaactc
     1861 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     1921 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     1981 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     2041 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     2101 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     2161 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
     2221 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
     2281 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
     2341 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
     2401 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
     2461 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
     2521 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
     2581 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
     2641 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
     2701 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
     2761 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
     2821 aggatcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac acggaacatt
     2881 taaaaaaatg aatgttttta tattctaaat tcgatatttt cgataggttt ggggttatgg
     2941 atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt ttgcgagctt
     3001 cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag cttgagcagg
     3061 agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc cgcgaaggaa
     3121 aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt cgtgaggctg
     3181 ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa acgttgctct
     3241 cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa gtgcgtttat
     3301 cgttgcaggc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat gaggccgatg
     3361 tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag ttgggtgaac
     3421 aatcgctggt gctggtggct tcaccacaaa ttgcagatgt cgattttact gagccgggaa
     3481 gacataacgc ctgtagcttt attatcaacg aaccgcaatg tgtcttccgg cagatatttg
     3541 agagcacgct gcgacagcgg cggatcacgg tggaaaacac cattgagctt ataagtatcg
     3601 aaagcatcaa gcgttgtgtc gcggcgaata tcggtgtcag ttacttgccc cactttgcgg
     3661 tggaaaaaga gattgagagc ggagaattaa ttgaattgcc ttttggcgaa cagtcgcaga
     3721 ccattacggc aatgtgcgca cattatgctg gaaaagcggt tagcccggcg atgcacattt
     3781 ttattcagtg tgttgaagag tgttttgtgg ttgcgtaaaa aaacaatgcc gggcttaaac
     3841 ccggcattga gattactctt ctgtcgtttg cggtggcacg cccgttccgc tcggtttacg
     3901 gcgcttacca atatttttgg tgtcgcgatg gcgttttttc acccgcggct tctctttctc
     3961 tttagctttt ttcttctcgg cgcgttttgc cagtactttc ttcgatggct tgccggtctg
     4021 cttctcgctt ggcgcacgcg ttttcgggcg taactcatca ataacgcgag ctttaatcgg
     4081 ctcttcaatg taacggccaa ccttgcccag cagcaaatgg tcatgggctt ccaccagcga
     4141 aattgcggtg cctttacgac cggcgcgcgc ggtacgtccg atacgatgca aataagtatc
     4201 gccactgcgc ggcatatcga agttaaagac gtggctgacg tcaggaatgt cgataccgcg
     4261 cgcggcaaca tcggtggcaa ccagtacgtt tacgcggcct tcggtcaggc gtttaattgc
     4321 ttcgttacgc ttgccttgta ccatctcacc ttcgagatag cagttgttga tgcccgcttc
     4381 gcgcagccag tttgccagct catgcacacg ttcacgctta cgcacaaaca cgattgagcg
     4441 agtcgcttca ggctgtttta acaggtgcac cagcaatgcg gttttatgtt ccaggtcatc
     4501 ggcgcggtaa taccactgat gaattttttt gcgctcacgg gtggacggat tggcagaaac
     4561 ttccaccgga tcttccagca gacgctcggc aaagtcctga atcgcatcgc cttccagcgt
     4621 tgccgaaaag agcagggtct gtttacgcca gcgagtttcg ccagcaatat gttcgatatc
     4681 ctgagcgaag cccatatcca gcatacggtc tgcttcgtcg aggatcagcg tttcaaccgc
     4741 gcggcaatcg aagttctctt cttttatgta ttgcagcaga cgtccggtcg tggcgaccac
     4801 gatgtcctga ttttcgctga acacttccgc gtggttcata taggctacgc cgccggtgat
     4861 ggtggcgata tccagatgcg tatgtttcgc cagttcgcgg gcatgatcgg ccacctgcat
     4921 cgccagctcg cgagttgggg tgaggatcaa aatacgcggc ggaccggatt tcttacgtgg
     4981 gaaatcgagc aggtgctgca acgctggcag cagatacgcc gccgttttac cggtgcctgt
     5041 cggcgcagaa ccgagtacat cacggccatc gagcgcaggc ggaatggcgg cagcctgaat
     5101 ggcggtcggg cgagtgaaac ctttatcctg gagggcttcc agcaggcttt cgtcgagttc
     5161 aagttcggaa aaagtcgtta cagtcatgtt ctacctctgt gtggggcgct gattatagac
     5221 gttacggctg caatcttcat ctgtttgtat ggatatcgct tctcgggtat tgttctcacc
     5281 cggtgctatc gccgtttttc ctgcaaggtt ttattttcat gtcacagtct acatccgtgc
     5341 ttcgtcgtaa tggatttact tttaaacagt tttttgttgc tcacgatcgc tgtgcgatga
     5401 aagtgggaac ggatggcatt ttgctgggcg catgggcacc ggtggctggg gtgaaacgtt
     5461 gccttgatat cggcgcgggt agcgggttgc tggcactgat gctggcgcag cgaaccagtg
     5521 acagcgtgat aattgatgcc gttgagctgg aaagtgaagc tgccgcgcag gcgcaggaaa
     5581 atatcaacca gtccccgtgg gcagagcgga ttaacgtcca tacggcggat attcagcagt
     5641 ggatcacaca gcagacagta cgcttcgatt taatcatcag taacccacct tactatcagc
     5701 agggagtaga gtgcgcgaca cctcaacggg aacaggctcg ctataccact acgcttgatc
     5761 acccatcgct gctgacctgt gcggcggagt gcattaccga agagggattt ttctgcgtgg
     5821 tgctgccaga gcagattggt aatggtttta ctgaactggc attaagcatg ggctggcatt
     5881 tacgtttgcg gacggatgtg gcggaaaacg aagcgcgact gccgcatcgg gtgctgctgg
     5941 cattctcccc gcaggcggga gaatgcttta gcgatcgctt agtcattcgt gggccagacc
     6001 agaactattc cgaagcgtat acggcgctga cccaggcttt ttatctgttt atgtaatgat
     6061 tgccggggga aaggatcgat ggaccggaat gcgtgagcag ttccgggtaa tccagcgtaa
     6121 aatgcagccc ccggctttct ttgcgcatca tcgcgcagcg aacaatcaac tcggcaacct
     6181 gtaccagatt acgcagctcc agcaaattat ttgagacgcg gaaatgggcg taatattcgt
     6241 ctatttcttg ttggagcatg gttatccgcc gcagggcgcg ttccaggcgc ttcgttgtgc
     6301 gcacaatgcc aacgtaatcc cacataaaca gccgtagctc gtgccagtta tgctgaatta
     6361 ctacccgttc gtcagggttc tcaacgcggc tttcatccca cggcggtaac gtactgacgc
     6421 cgtgggcata aggcatacgt ctggtgatat cttctgccgc cgaccagcca tagaccagac
     6481 actccagcaa tgaattcgag gccatgcggt tagcgccgtg taagccggta taactcacct
     6541 cgccaatggc atacaagccc tcgacgtccg tacgcccatg atcatcaacc attacaccac
     6601 ctcaggtata gtgtgcagca ggcacaatcg gtaccggttc ttgtgtgaga tcaatcccca
     6661 gcccgagcaa tttttcataa atcatcggga aatgctggcg aataaaatcg gcgggcttat
     6721 ggctgatgtc gaggaacata caatctgcgc cgaggcgttt catttcatgg tcaatggcgc
     6781 gggcgacaat atcgcgcggg gccagttcgc cgcgctcatc aaaatcgggc ataaaacgcg
     6841 tgccatccgg gcgcttgaga taagcgcctt cgccgcgcag tgcttccgtt aacaggaaat
     6901 tgcgtgcctg tgggtgatac agtgcggtag ggtggaactg attaaattcg agattggcaa
     6961 cccgacagcc cgcgcgccac gccatagcaa tgccatcgcc agaagaaata tccggattgg
     7021 tggtgtactg atacaccttc gacgcaccgc cggttgccag taccactgct tttgcgtggc
     7081 aggtttccac cgtttcttta tttcggttcc atacccacgc gcctacaacc cgtcgcgtgc
     7141 ccggcaggcc aattttgtca gagacaatca gatcaactgc gttgctgcgc tccagcacac
     7201 gaatattcgg atggttctgc gccttgctca ccagcgtgga ttgtacttct ctaccggtgg
     7261 cgtcggcggc atgaagaata cgacggtgac tatgtccacc ttcacgggtc agatggtagc
     7321 tttcttcgcc attcggttga acgtgggtat caaacaaaac cccctggtca attaaccatg
     7381 gcacacagga acgagcattg ctggcgacaa attcaactgc atggtgatcg caaataccag
     7441 ccccggcaat caatgtgtct tccacatgcg agtcaatgct gtcagtttca tcaaacacgg
     7501 cggcaatacc gccctgggca taaaatgttg aaccttccgt taccgggcct ttacttagaa
     7561 cgatgacctg atgctggtca gccaggcgta gcgccagtga aagtccggct gcgccgctac
     7621 caataatcaa cacgtcacat gaatgttcag ggagagtatt cattttcttt gtttaattta
     7681 ctaaacattg tttggtcagc atagcatcat gttgtgcgaa taaacacctg ctattttaat
     7741 atttgttaca gtggctaaac acgttgacgc agggcggcga gaaaaagaga agttactggc
     7801 tggtggagga ttaggtggtg aaataaaaag gccgttgggt tactcttcag gcagttaaat
     7861 gggcatttct acacagataa tgcgatgttc agattctgta gacttataat gatagataat
     7921 gatccgtcta cagcatgaca aacaaaaaca gatgcgttac ggaactttac aaaaacgaga
     7981 cactctaacc ctttgcttgc tcaaattgca gctaatggag tggcgtttcg atagcgcgtg
     8041 gaaatttggt ttggggagac tttacctcgg atgagcgagc agttaacgga ccaggtcctg
     8101 gttgaacggg tccagaaggg agatcagaaa gcctttaact tactggtagt gcgctatcag
     8161 cataaagtgg cgagtctggt ttcccgctat gtgccgtcgg gtgatgttcc cgatgtggta
     8221 caagaagctt ttattaaagc ctatcgtgcg ctggattcgt tccggggaga tagcgctttt
     8281 tatacatggc tgtatcggat tgctgtaaat acagcgaaaa attacctggt tgctcagggg
     8341 cgtcgtccac cttccagtga tgtggatgcc attgaagctg aaaacttcga aagtggcggc
     8401 gcgttgaaag aaatttcgaa ccctgagaac ttaatgttgt cagaagaact gagacagata
     8461 gttttccgaa ctattgagtc cctcccggaa gatttgcgca tggcaataac cttgcgggag
     8521 ctggatggcc tgagctatga agagatagcc gctatcatgg attgtccggt aggtacggtg
     8581 cgttcacgta tcttccgagc gagggaagct attgataaca aagttcaacc gcttatcagg
     8641 cgttgacgat agcgggatac tggataaggg tattaggcat gcagaaagaa caactttccg
     8701 ctttaatgga tggcgaaacg ctggatagtg agctgcttaa cgaactggct cataacccag
     8761 aaatgcagaa aacctgggaa agctatcact taatccgtga ttcaatgcgg ggtgatactc
     8821 ccgaggtgct ccatttcgat atctcttcac gcgtgatggc cgccattgaa gaagagccag
     8881 tacgtcaatc ggcgacattg atcccggaag cccagcctgc gccgcatcaa tggcagaaaa
     8941 tgccattctg gcagaaagta cgtccgtggg cggcacagct tacccaaatg ggcgtagccg
     9001 catgcgtatc gcttgcagtt atcgttggcg tccagcacta taatggacaa tctgaaacgt
     9061 cccagcagcc cgaaacgccg gtatttaata cactgccgat gatgggtaaa gccagcccgg
     9121 taagcctggg agtaccttct gaagcgaccg caaacaatgg tcaacagcag caggtacagg
     9181 agcagcgtcg tcgcattaat gcaatgttgc aggattacga actgcaacgc cgactccact
     9241 ctgaacagct tcagtttgag caggcgcaaa cccagcaagc cgctgtacag gtgccaggaa
     9301 ttcaaacttt aggaacgcaa tcgcagtaat gaagcaactt tggtttgcca tgtcattagt
     9361 gacaggtagc ctgttattct ctgctaacgc ctcggccact cccgcgtccg gggcgttatt
     9421 acagcagatg aacctggcca gtcagtcact gaattacgag ctgtcattca tcagcatcaa
     9481 taaacagggt gttgagtctc tgcgttatcg acatgcacgc ctcgataacc gtcctcttgc
     9541 acaattgttg caaatggatg gcccgcgccg ggaagtggta cagcgcggca atgaaatcag
     9601 ctattttgaa ccgggacttg aaccgttcac gcttaatggc gattacattg ttgattctct
     9661 gccatcgctt atctataccg atttcaaacg cctttctcct tactacgact ttatctccgt
     9721 cgggcgcacg cgtattgctg atcgtctttg cgaagtcatt cgcgtggttg cccgagatgg
     9781 tacacgctac agctacatcg tgtggatgga caccgaatcg aaattaccga tgcgggttga
     9841 tctccttgat cgcgatggtg aaacgctgga acaatttcgc gtgattgctt ttaacgtcaa
     9901 tcaggatatc agcagcagta tgcagacgct ggcgaaggca aatttgccgc cgttgctttc
     9961 tgttcctgta ggtgaaaaag ctaaattcag ctggacgcca acctggttgc cacagggttt
    10021 tagcgaagtt tccagtagtc gacgtccgct accgacgatg gacaacatgc ctatcgaatc
    10081 acgtctctat tccgacggat tattcagctt ctcggtaaac gttaaccgcg ctacgccatc
    10141 gagcaccgat cagatgttgc gcaccggacg cagaaccgtc agtacaagcg tacgtgataa
    10201 cgccgaaatc accattgtcg gtgaactgcc gccgcaaacg gcgaaacgca ttgccgagaa
    10261 tattaagttc ggggcagcgc aatgatcaaa gagtgggcta ccgtcgtctc ctggcaaaac
    10321 gggcaggcgc tggtcagttg tgatgttaaa gcttcatgca gcagctgcgc ttcacgcgcc
    10381 ggttgcggta gccgcgtgtt gaataaactt ggcccgcaaa ccacgcatac cattgtcgta
    10441 ccctgtgatg agccgttagt gccggggcaa aaagtggaat tggggatcgc cgaaggcagc
    10501 ctgcttagct ccgcattact ggtttatatg tcgccgctgg tgggattatt cctcatcgct
    10561 tcgctatttc agctactctt tgcttctgac gtcgcagcat tatgtggtgc ggtcctcggc
    10621 ggtattggcg ggttcctgat tgcgcgcggc tactcgcgta agtttgctgc ccgggcagaa
    10681 tggcaaccga tcatcttaag cgtggcattg ccgcctggcc ttgtgcgatt tgaaacatct
    10741 tcggaagacg cgagccagtg atttcactgg ctttgttgca ttttgcataa caaatgcgag
    10801 gacgtttcca gataccacaa tccatacggt atgaacattt cctcgcctca atgttgtagt
    10861 gtagaatgcg gcgtttctat taatacagac gttaagctca gaacagcgac ttctaaaagc
    10921 ctggttaacc gggcattaag gcacaataat catactttat gaagaatata cgtaactttt
    10981 cgatcatagc tcacattgac cacggtaaat cgacgctgtc tgaccgtatt atccagatct
    11041 gcggtggcct gtctgaccgt gaaatggagg cgcaggttct cgattccatg gatcttgagc
    11101 gtgagcgtgg cattaccatc aaagcgcaaa gcgtgacgct ggactacaaa gcgtctgacg
    11161 gcgaaaccta tcagcttaac tttatcgaca ccccgggcca cgtagacttc tcctatgaag
    11221 tttcccgttc gctggctgcc tgtgaaggtg cattgctggt ggtcgacgcc gggcagggcg
    11281 tagaagcgca aaccctggca aactgctaca ccgccatgga aatggatctc gaagtggtgc
    11341 cggtactgaa caagattgac ctgccggcag ccgatcctga acgcgtggcg gaagaaattg
    11401 aagatatcgt cggcatcgac gccaccgatg cagtgcgctg ttcagcgaaa accggcgttg
    11461 gcgtgcagga cgttctcgaa cgtctggtgc gcgacattcc gccgccggaa ggcgatccgg
    11521 aaggcccgtt gcaggcacta attatcgact cctggttcga caactacctg ggcgttgttt
    11581 cacttatccg tattaaaaac ggcaccctgc gtaagggcga caaagtgaaa gtcatgagta
    11641 ccgggcagac ctataacgcc gaccgtctgg gcatcttcac gccgaaacag gttgaccgca
    11701 ctgaactgaa atgtggcgaa gtaggctggc tcgtatgtgc gattaaagat atccacggcg
    11761 ctccagtcgg cgatacctta acgctggcgc gtaatccggc agaaaaggcg ctgcctggct
    11821 ttaagaaagt caaaccgcag gtatacgccg gtctgttccc ggtaagttcc gacgactatg
    11881 aagccttccg tgacgcgctg ggtaaactca gcctgaacga tgcctcactg ttctatgagc
    11941 cggaaagctc cagcgcgctg ggctttggtt tccgctgcgg cttccttggc ctgctgcaca
    12001 tggagatcat ccaggagcgt ctggaacgtg aatacgatct ggatctgatc accactgcgc
    12061 cgaccgtagt gtatgaagtt gaaaccacgt caagggaagt tatctacgtc gacagcccat
    12121 ccaagctgcc tgcggtaaat aacatttacg aactgcgcga gccgattgca gagtgtcaca
    12181 tgctgctgcc gcaggcatat ctcggcaacg ttattacgct gtgcgtagaa aaacgcggcg
    12241 tgcagaccaa tatggtttac cacggtaatc aggtggcgct gacgtacgag atcccgatgg
    12301 cggaagtggt gctcgacttc ttcgatcgcc tgaaatctac ctcgcgtggt tatgcgtctc
    12361 tggattacaa cttcaaacgc ttccaggcgt ccgacatggt acgtgtagac gtattaatca
    12421 acggtgaacg tgttgatgcg ctggcgttga tcacccaccg tgataattcg caaaaccgag
    12481 gtcgcgagtt ggtggagaag atgaaagatc tgatcccacg ccagcagttt gatatcgcca
    12541 ttcaggcagc gattggtacg cacattattg cgcgatccac cgtgaaacag ctgcgtaaaa
    12601 acgtactggc taaatgttat ggcggcgata tcagccgtaa gaaaaagctg ctgcagaagc
    12661 agaaagaagg taagaaacgc atgaagcaga tcggtaacgt tgagctgccg caggaagcgt
    12721 tcctcgccat tctgcacgtc ggcaaagaca acaaataacc cttaggagtt ggcatggcga
    12781 atatgtttgc cctgattctg gtgattgcca cactggtgac gggcatttta tggtgcgtgg
    12841 ataaattctt tttcgcacct aaacggcggg aacgtcaggc agcggcgcag gcggctgccg
    12901 gtgactcact ggataaagca acgttgaaaa aggttgcacc gaagcctggc tggctggaaa
    12961 ccggagcttc tgtttttccg gtgctggcta tcgtattgat tgtacgttcg tttatttatg
    13021 aaccgttcca gatcccgtca ggttcgatga tgccgactct gttaatcggt gattttattc
    13081 tggtagagaa gtttgcttat ggcattaaag atcctatcta ccagaaaacg ctgatcgaaa
    13141 ccggtcatcc gaaacgcggc gatatcgtgg tctttaaata tccggaagat ccaaagcttg
    13201 attacatcaa gcgcgcggtg ggtttaccgg gcgataaagt cacttacgat ccggtctcaa
    13261 aagagctgac gattcaaccg ggatgcagtt ccggccaggc gtgtgaaaac gcgctgccgg
    13321 tcacctactc aaacgtggaa ccgagcgatt tcgttcagac cttctcacgc cgtaatggtg
    13381 gggaagcgac cagcggattc tttgaagtgc cgaaaaacga aaccaaagaa aatggaattc
    13441 gtctttccga gcgtaaagag acactgggtg atgtgacgca ccgaattctg acagtgccga
    13501 ttgcgcagga ccaggtgggg atgtattacc agcagccagg gcaacaactg gcaacctgga
    13561 ttgttccgcc gggacaatac ttcatgatgg gcgacaaccg cgacaacagc gcggacagcc
    13621 gttactgggg ctttgtgcct gaagcgaatc tggtcggtcg ggccacggct atctggatga
    13681 gcttcgataa gcaagaaggc gaatggccga ctggtgtgcg cttaagtcgc attggcggca
    13741 tccattaata gccatcttcg ttcacgttgt cgccgttatg gcgacaacgt gaattattta
    13801 tgagataaat ctcccgtggc taacgacatc ccccgtcgtt gtgtatagaa tattcccccg
    13861 aagtttaagg ttggcacctc caggttgcca cggcacacga aacagcgttg gtccccatat
    13921 accgctaaac tgaaactgca gcgaagcagt tagcagagcc atgtatatca ggtctgtttc
    13981 gtgtgctgaa ttgttgacgc atttatttat tggtatcgca tgaaccccat cgtaattaat
    14041 cggcttcaac ggaagctggg ctacactttt aatcatcagg aactgttgca gcaggcatta
    14101 actcatcgta gtgccagcag caaacataac gagcgtttag aatttttagg cgactctatt
    14161 ctgagctacg ttatcgccaa tgcgctttat caccgtttcc ctcgtgtgga tgaaggcgat
    14221 atgagccgga tgcgcgccac gctggtccgt ggcaatacgc tggcggaact ggcgcgcgaa
    14281 tttgagttag gcgagtgctt acgtttaggg ccaggtgaac ttaaaagcgg tggatttcgt
    14341 cgtgagtcaa ttctcgccga caccgtcgaa gcattaattg gtggcgtatt cctcgacagt
    14401 gatattcaaa ccgtcgagaa attaatcctt aactggtatc aaactcgtct ggacgaaatt
    14461 agcccaggcg ataaacaaaa agatccgaaa acgcgcttgc aagaatattt gcagggtcgc
    14521 catctgccgc tgccgactta tctggtagtc caggtacgtg gcgaagcgca cgatcaggaa
    14581 tttactatcc actgccaggt cagcggcctg agtgaaccgg tggttggcac aggttcaagc
    14641 cgtcgtaagg ctgagcaggc tgccgccgaa caggcgttga aaaaactgga gctggaatga
    14701 gcatcgataa aagttactgc ggatttattg ccatcgtcgg acgtccgaac gttggcaaat
    14761 ccacattgtt gaacaaactg ctggggcaga aaatctccat cacctcccgc aaggcgcaga
    14821 caactcgtca ccgcattgtg gggatccata ctgaaggcgc gtatcaggcg atctacgtcg
    14881 atacaccggg cctgcatatg gaagaaaaac gcgccattaa ccgcctgatg aacaaagcgg
    14941 cgagcagctc tattggcgat gttgagctgg tgatttttgt cgttgaaggc acccgctgga
    15001 cgccggacga cgaaatggtg ctcaacaaac tgcgcgaagg caaagcgccg gtaatcctcg
    15061 cggtgaacaa agtggacaac gtgcaggaga aagccgatct gctgccgcac ctgcagttcc
    15121 tggcaagcca gatgaacttc ctcgatatcg tgccaatctc tgccgaaacc gggctgaatg
    15181 ttgacactat tgcggcaatc gtgcgtaagc atctgcctga agcgactcat cacttcccgg
    15241 aagattacat caccgatcgc tcacagcgtt ttatggcgtc tgaaatcatc cgcgaaaaac
    15301 tgatgcgttt ccttggcgct gaactgccgt actccgtgac cgtggagatc gaacgtttcg
    15361 tctctaacga acgcggcggt tgtgacatca acggtttgat tctcgttgag cgtgaagggc
    15421 agaagaagat ggttattggc aacaaagggg ccaagatcaa aaccatcggg attgaagcgc
    15481 gtaaagacat gcaggaaatg ttcgaagcgc ctgtccacct tgaactgtgg gtaaaagtga
    15541 aatcgggttg ggccgacgac gaacgcgcac tgcgcagtct cggttatgtt gacgatcttt
    15601 aagagtcact ccgatggaag gctggcagcg cgcatttgtc ctgcatagtc gcccgtggag
    15661 cgaaaccagc ctgatgctgg acgtcttcac agaggaatcg ggtcgcgtgc gtctggttgc
    15721 caaaggcgca cgctctaaac gctctactct gaaaggtgca ttacagcctt tcacccctct
    15781 cttgctacgt tttggcgggc gtggcgaagt caaaacgctg cgcagtgctg aagctgtctc
    15841 actggcgctg ccattaagcg gtatcacgct ttacagcggt ctgtacatca acgaacttct
    15901 ctcccgcgta ctggaatacg agacgcgctt ctctgaactc tttttcgatt acttgcactg
    15961 cattcagtct cttgcaggtg acactggtac gccagaaccc gcgctgcgtc gctttgaact
    16021 ggcactactc gggcatctgg gctatggcgt caattttacc cattgcgcgg gtagcggcga
    16081 gccggtagat ggcaccatga cgtatcgtta tcgcgaagaa aaagggttta tcgcaagcgt
    16141 cgttatcgac aataaaacgt tcaccggaag gcagttaaaa gcgttaaacg cgcgggaatt
    16201 tcctgacgca gacacactac gcgccgcgaa acgctttacc cgaatggcgc ttaagccgta
    16261 tcttggcggt aaaccattaa agagcaggga actgttccgg cagtttatgc ctaagcgaac
    16321 ggtgaaaaca cattatgaat gatgaggatt gtcatggctg aattactgtt aggcgtcaac
    16381 attgaccata ttgcaacgct gcgtaacgca cgcggtactg cttacccgga tccggtgcag
    16441 gcggcgttta ttgccgagca ggcgggagcg gacggcatta ccgtgcattt acgtgaagat
    16501 cgccgccaca ttactgaccg cgatgtgcgc atcctgcggc agacgctgga tacccgaatg
    16561 aatctggaga tggcagtgac cgaagagatg ctggcgatcg ccgttgagac gaagccacat
    16621 ttttgctgct tggtaccgga aaagcgtcag gaagtaacaa ccgaaggcgg cctggacgtt
    16681 gcagggcagc gtgaaaaaat acgcgatgcc tgcaaacgtc tggcagatgc cgggattcag
    16741 gtttctctgt ttatcgacgc cgatgaagag cagatcaaag ctgcggcaga ggttggcgca
    16801 ccgtttatcg agatccacac cggttgctat gctgatgcca aaactgacgc cgaacaggcg
    16861 caagagctgg tgcgtatcgc caaagccgcg acctttgccg ctagtctcgg tctgaaagtt
    16921 aacgccggac acggtctgac ctatcacaac gtgaaagcca ttgccgccat ccctgagatg
    16981 catgaactga atatcggtca tgccattatt ggccgtgcag tgatgaccgg actgaaagat
    17041 gcggtggcag aaatgaagcg tctgatgctg gaagcgcgtg gctaatggca atattaggtt
    17101 taggcacgga tatcgtagag atcgctcgca tagaagcagt gatcgcccga tccggtgagc
    17161 gcctggcacg ccgcgtatta agcgataacg aatgggcaat ctggaaaacg caccaccagc
    17221 cggtacgttt tctggcgaag cgttttgctg tgaaagaagc cgcagcaaaa gcgtttggca
    17281 ccgggatccg caatggtctg gcgtttaatc aatttgaagt attcaatgat gagctcggca
    17341 aaccacggct acggctatgg ggtgaggcat taaaactggc ggaaaagctc ggcgttgtaa
    17401 atatgcatgt aacgctggca gatgaacgcc actatgcttg tgccacggta attattgaaa
    17461 gttaaaataa taccgttctc tttaaaaagt aaaacccccg aactggtaag gaacgggggt
    17521 tttacttttt aactttaatt ttgtgcagca tgcaccctca acaacagcga ttatggatca
    17581 gtgtacttat cttttacaag caacttgcaa tctttagcat aaaaactcga gcctttacga
    17641 agaaagcaat attgatggaa agattaacgt gaccgccaat tcgtaagtac attaaaattg
    17701 gcttcgttat tgaagatttt gctgtgcttt acaccatgcc acagaattcc cccattgaaa
    17761 cgagtggtgt cgtcaaagct ctggtgtgga gtgcagcatg caccctcaat aactcgcacg
    17821 ttcagttttg gggagatgta agggctaatc tgaatggctg cattccttgt ttaaggaaaa
    17881 acgaatgact gattgccgat acctgattaa acgggtcatc aaaatcatca ttgctgtttt
    17941 acagctgatc cttctgttct tataacacaa ggaaacgtac ttaaggtgcg tccggtgaac
    18001 caatcggacg cgcctttaat aactataaat aagtgtcttg gcagatacta tataaattaa
    18061 cttagtgaat gattatgcta atgtcatcaa ttaaataaat ataatggcgt taaggcttcc
    18121 cagtaatata attaatactc tacttccaga gtagaatatt aaattttatc cgcgtggtgc
    18181 atcagcacaa atttatccca caactgttct tctgtctcga catgcgccgg atctttcaca
    18241 atagtattgg ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat
    18301 tcggtacact tatcgctgtt aatttcgtag atatgatctc ccattgaaat cgcctcattc
    18361 gggcattcgg gttcacacat atcacaatta atacagcgtt tagtaattag taaagacatt
    18421 tcaatggatt accgttaaat cattttaaaa tcagtaagtt gtatcgagtt tgtatgcttt
    18481 actgtcatta acttactgta tgttgatcca gtgtatttaa ccttgataaa ctcagtccag
    18541 caacacaaaa ccgcaacaca ttgcattttg tcccgtagaa aagacttgta tgtgtgagct
    18601 tgttttctgc gcctacgcag ataaggattg agaatgccgc gcactgtaac acataatccg
    18661 gatagcccca ataatgacga tgttttagcc gcatctgaaa aatgggacgc ctgtaaaccc
    18721 ccctatacca gcgcacacat gaaaatctgt gttgctgccg ccaaaatcat cctcgctgct
    18781 tccggcgtgg ctcgccgttc caaatacgaa aaagagaact atctccgtat cgatttcagc
    18841 aaagccggta aggttacatt ttacgccgag tttccaaaaa agatgggcct caaaggtaaa
    18901 aagctcggcg agtggccgga gctcgttatc cagctggcgc gcgaaaaagc actaggtatg
    18961 gctgacggtg gactgcgggc agagtccgta catgcagcgc tggaaatgta ccgggatgac
    19021 ccaaagccaa agtagcccgg cagaagctga gcccggacag tttcacaacc tacggggtgc
    19081 gtatcgaccg gattaaagca acgttcggcg agcgtgaggt gttcagcgac gtaacataca
    19141 atcggctggt ggaagtgctg gacgagtgga tcgccactcg ctcgaacaat aacgccctgg
    19201 agttgtttgc cgagctccgt cggttctgga agttctgcgc acctactctt tgcaacggcc
    19261 gcaatgttgc cgccagtctg ccagatgatt atgtttcctc ccgcgtacag aaacctaccc
    19321 ccacacggct ttttaccgat attgaatcaa tcgcccgact ctggctcaat gttgctgcct
    19381 gcacctctgt acaccagaag aatgctgttc gcttcatgat catcactggt gttcgtccga
    19441 ttaatgtcca taacctgcgc tgggactacg tttacgagga ggctggtgaa attgtttatc
    19501 cggaaggggt tatcggcatg cgaggggcta tgaaaacaca aaaggctttc cgcctgccga
    19561 taacgcctga gatccggcgg attatcgacg agcagaaagc ctggcgtgat tcagttcctg
    19621 agtgcaacag ggattatgta tttttgcagc cacgtgatcc aatgcagcca ttttcaaaac
    19681 gatcactgga taagctggtg aaaacataca gcccggacgg ggctgtaa
//
LOCUS       DADLCQ010000077        19652 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000077 DADLCQ010000000
VERSION     DADLCQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.077"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="JQG95_002534"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="JQG95_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY5889050.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /locus_tag="JQG95_002535"
     CDS             complement(1206..1607)
                     /locus_tag="JQG95_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY5889051.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JQG95_002536"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="JQG95_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY5889052.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JQG95_002537"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="JQG95_002537"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY5889053.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="JQG95_002538"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="JQG95_002538"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY5889054.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5795)
                     /locus_tag="JQG95_002539"
                     /pseudo
     CDS             complement(5235..5795)
                     /locus_tag="JQG95_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="JQG95_002540"
     CDS             complement(5911..6954)
                     /locus_tag="JQG95_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY5889055.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            complement(7200..7652)
                     /locus_tag="JQG95_002541"
     CDS             complement(7200..7652)
                     /locus_tag="JQG95_002541"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5889056.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JQG95_002542"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="JQG95_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY5889057.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JQG95_002543"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="JQG95_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY5889058.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JQG95_002544"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="JQG95_002544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY5889059.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JQG95_002545"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="JQG95_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY5889060.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10264..12003
                     /gene="flhA"
                     /locus_tag="JQG95_002546"
     CDS             10264..12003
                     /gene="flhA"
                     /locus_tag="JQG95_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY5889061.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIEADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            complement(12057..12670)
                     /locus_tag="JQG95_002547"
                     /pseudo
     CDS             complement(12057..12670)
                     /locus_tag="JQG95_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12962..13702
                     /locus_tag="JQG95_002548"
     CDS             12962..13702
                     /locus_tag="JQG95_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY5889062.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13673..14440)
                     /locus_tag="JQG95_002549"
     CDS             complement(13673..14440)
                     /locus_tag="JQG95_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY5889063.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWHLFCLGERVV"
     gene            complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="JQG95_002550"
     CDS             complement(14646..15224)
                     /gene="lpcA"
                     /locus_tag="JQG95_002550"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY5889064.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15464..17908
                     /locus_tag="JQG95_002551"
                     /pseudo
     CDS             15464..17908
                     /locus_tag="JQG95_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(17951..18424)
                     /gene="ivy"
                     /locus_tag="JQG95_002552"
     CDS             complement(17951..18424)
                     /gene="ivy"
                     /locus_tag="JQG95_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY5889065.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            18578..19348
                     /gene="yafV"
                     /locus_tag="JQG95_002553"
     CDS             18578..19348
                     /gene="yafV"
                     /locus_tag="JQG95_002553"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY5889066.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            19458..19652
                     /locus_tag="JQG95_002554"
                     /pseudo
     CDS             19458..19652
                     /locus_tag="JQG95_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541010.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         4787 a         5052 c         5069 g         4744 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcattatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agtttcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtaaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggga tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact gatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgcccagac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggatgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacgttcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 aaaatcatct cttccgtcaa aatcctgcgc gcgcctaaaa aatggtcctg ccagaaaggt
    12121 tcggctaacc ggcttacccg aatggtttcc ccggtgcgca gtgactcaat aaactgaccg
    12181 tcgcccagat acacccccat atggtcggca atctcgcggc tgtggatatg gaaaaatagc
    12241 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta gtggtacatc
    12301 tcattggccg tgcgcgggag tttcgcctca aggatcttgt tgtaggcata aaaaactaac
    12361 ccactacaat cgaagccttg atcgggccgc gtgccgcccc agacgtaagg cttgccaagc
    12421 tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gcaggtgatc gctgcttaaa
    12481 aagtggttgt tttccgccag cgcctgccag cgtctgtcac tcgccttgag cggcgcagga
    12541 aaccattctg gatgctgttt tatctgctcc cggttgtggt ggcgctggcg agtaaaggga
    12601 gcaaaacgca taacgaaaac acgcctggat gcaaaaatct gccgagcaag aacgaagaca
    12661 tgaaagacat aaaccggctg aatgaaggtg agaattttaa aagaccggag gagtaaacgg
    12721 gattattaat tgcttatttg catattacta ttgagatttc ataatattag gaaataaatg
    12781 atacatgcgc ttttcggact attgaaaact gcccctgtgc taaatttgta aacccagcgc
    12841 cgacaggctt gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc
    12901 cgatttggga tccattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag
    12961 tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg
    13021 tttgctgggc agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat
    13081 gggatcccct gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tttacgtcaa
    13141 aatgggcgag caatatcaac tgctcgacag ttataaaatc tgtaaatatt ccggcggctt
    13201 agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg
    13261 taatcagtta aaaccagaca gccgttacta caaagcgatt aatattggat tccccaatgc
    13321 ttatgaccgt gcgcatggtt atgaagggaa atacctgatg atccacggcg attgtgtatc
    13381 catcggctgc tacgcaatga ccaatcaggg tattgatgag atcttccagt tcgttactgg
    13441 tgcgctggtg tttggtcagc cgagcgtgca ggtgagtatt tacccattcc gcatgaccga
    13501 cgccaatatg aagcgccata aatattccaa ctttaaggac ttctgggagc agctgaagcc
    13561 gggctacgac tactttgagc aaacccgtaa gccaccgacc gtttctgtgg ccaatggccg
    13621 ttacgtggtc agcaagccgt taagccacga agtggtgcag ccacaactgg catcaaacta
    13681 cacgctcccc gaggcaaaat aagtgccatt cgcctggcat aattttttgc caggtttcat
    13741 tgcccgtcag cggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc
    13801 tgaagtcgat ttccacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg
    13861 tgatccagtg taaattagtc gagcaatacg ccattacata gcgcccgtcc gaaagcagca
    13921 tgttgaaaac gcccttcgcc cgcagttcat ccgccagtga ggcgatatat ttaaacactg
    13981 ccgccatgtt gcccggcgtg cgcgggtaac gctgcgttaa tttatgcagc agccagcaaa
    14041 aggctttttc gctgtcggtt tcaccgaccg ggcggaagtt gccggtttcc agtgatttgt
    14101 agcccgtcag ttgtccgtta tgggcataag tccagttacg cccccataac tcgcgggtaa
    14161 atgggtgagt attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca
    14221 ccgaacagga ttttatcggg tagtcctgga caagtttggc gatgggggaa ttaaagctgg
    14281 gttgtggatc tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat
    14341 ctttatgtgg cccggttcca ccaccacgct gtacaagtcc ggtgaaacta aagcagatat
    14401 cggtagggac gttggcgctc atcccgagca attcgcacat gggtaacctc cacaacagcg
    14461 ggagcgcatg gaccccggcg aagattgatt taagaatttt caggtcggat gcggcgtaaa
    14521 cgaattattc ggcctacggt tcgggtacag atttgtaggc atgataagac gcgtcagcat
    14581 cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa acgccttatc cggcctacgc
    14641 cagacttact taaccatctc tttttcaatt aactggatca ggatatggat cactttaatg
    14701 tgaatctcct gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc gatatccgcc
    14761 gtgccagcca ttttaccgcc gtctttaccg gtcagggtga tcactttcat ccccttctca
    14821 cgcgccgctg cgatcgcttt gatcacgttt gcagagttac cggaggtgga gatccccagc
    14881 agtacatcgc cttcgcgacc taccgcttca acgtagcggg agaaaatatc attgaaaccg
    14941 aaatcattac cgacgcagga aatatgacta acgtcagaaa tagcaatcgc cgggtagccc
    15001 ggacggtttt cacggtagcg accggtcaac tcttcggcaa agtgcatagc gtcgcaatgg
    15061 gaaccgccgt tgccgcagga aagcactttg ccaccggctt taaagctgtc tgctaacagg
    15121 accgccgcgc gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt agccagcgtt
    15181 tccgccgctt cgttcagttc gttacgaata agatcctggt acatgaggat atccttcagc
    15241 ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga agtacgggca
    15301 ggtgctatga ccaggacttt ttgacctgaa gtgcggataa aaacagcaac aatgtgagct
    15361 ttgttgtaat tatattgtaa acacattgct aaatgttttt acatccacta caaccatatc
    15421 atcacaagtg gtcagacctc ctacaagtaa ggggcttttc gttatgatga ttttgagtat
    15481 tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct tatttatcag
    15541 cagtctgatt ttgctcgcct agacagccgc cctcggcgtt gctggtctgt ggtcggcgtg
    15601 ggtactggtg cctctgacca ttatcctcgt gccatttaac tttgcgccta tgcgtaagtc
    15661 gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga tgtcgcgcac
    15721 tgagaaagaa gcgattgatg cgggcaccac ctggtgggag ggcgacttgt tccagggcaa
    15781 gccggactgg aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg aagagcaagc
    15841 gtttctcgac ggcccggtag aagaagcctg ccggatggcg aatgatttcc agattactca
    15901 tgagctggcg gatctgccgc cggagttgtg ggcgtacctt aaagagcatc gcttcttcgc
    15961 gatgattatc aaaaaagagt acggcggact ggagttctca gcttatgccc agtctcgtgt
    16021 gctgcaaaaa ctctctggcg tgagcgggat cctggcgatt accgtcggcg tgccaaactc
    16081 attaggcccg ggcgaactgt tgcaacatta cggcactgac gagcagaaag atcactatct
    16141 gccgcgtctg gcgcgtggtc aggagatccc ctgctttgca ctgaccagcc cggaagcggg
    16201 ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc atgggcgaat ggcagggcca
    16261 gcaggtgctg gggatgcgtc tgacctggaa caaacgctac attacgctgg caccgattgc
    16321 gaccgtgctt gggctggcgt ttaaactctc cgacccggaa aaattactcg gcggtgcaga
    16381 agatttaggc attacctgtg cgctgatccc aaccaccacg ccgggcgtgg aaattggtcg
    16441 tcgccacttc ccgctgaacg taccgttcca gaacggaccg acgcgcggta aagatgtctt
    16501 cgtgccgatc gattacatca tcggcgggcc gaaaatggcc gggcaaggct ggcggatgct
    16561 ggtggagtgc ctctcggtag gccgcggcat caccctgcct tccaactcaa ccggcggcgt
    16621 gaaatcggta gcgctggcaa cgggcgcgta ttctcacatt cgccgtcagt tcaaaatctc
    16681 tatcggtaag atggaaggga ttgaagagcc gctggcgcgt attgccggga acgcttatgt
    16741 aatggatgct gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt
    16801 gctgtcggct atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc
    16861 gatggatatt accggcggta aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc
    16921 ttaccagggc gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat
    16981 gatgatcttc ggacaaggag cgattcgtta ccatccgtac gtgctggaag agatggaagc
    17041 ggcgaagaac aatgacgtca acgcgttcga taaactgttg ttcaaacata tcggtcacgt
    17101 cggtagcaac aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac
    17161 gccaaccggc gatgccacta aacgctacta tcagcacctg aaccgcctga gcgccaacct
    17221 cgccctgctt tctgatgtct cgatggcact gctgggcggc agcctgaaac gtcgtgagcg
    17281 tatctcggca cgtctggggg atattttaag ccagctttac ctcgcctctg ccgttctgaa
    17341 gcgttatgac gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact ggggcgtaca
    17401 ggatgcgctg tatcaggcag aacaggcaat ggacaattta ctgcaaaact tcccgaaccg
    17461 cgtggttgcc gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc
    17521 gtctgacaag ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccactcgttc
    17581 acgtattggt cgcggtcagt acctgacgcc aagcgagcat aacccggttg gtttactgga
    17641 agaggcgttg gtggatgtga ttgccgccga cccgattcat cagcggatct gtaaagagct
    17701 gggtaaaaat ctgccattta cccgtctgga tgaactggcg cacaacgcgc tgacgaaggg
    17761 gctgattgat aaagatgaag ccgctattct ggtgaaagcg gaagaaagcc gtctgcgcag
    17821 tattaacgtt gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga
    17881 gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt
    17941 tatccgctaa ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg
    18001 ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc gttcacattc agccaggtga
    18061 gtttctcttg cgacgttttc tcatcaatag ccgagaacag ccccgtcatc tgattagatt
    18121 tctcggacca catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca
    18181 tcacctgata cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt
    18241 tcatcaccca tgcaggtagc ttatgccctt gcaccatctg attaaacgca gctttggtgg
    18301 tttcgccctt tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga
    18361 tgaccagtgc ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc
    18421 ccatgttatt aacctcctaa agcctggcga taacacgata atttgcaaat cagatatttc
    18481 caatatcagc atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta
    18541 aagttataaa acccttcgca taaaggagtt gtacaccgtg cctagtttga agattacgct
    18601 tttgcagcaa ccgttggtgt gggtggatgg tcctgccaac ctgcgccatt ttgatcgtca
    18661 actggaaggt attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt
    18721 tgccatggaa gcggcagctt cgtcgcttgc acaagatgac gtagtgaact ggatgacagc
    18781 taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa cggagtctgg
    18841 ttcggttaac cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg
    18901 tcatctgttc cgcatggcag atgagcatct gcattataaa gcgggcaatg cgcgagtgat
    18961 tgtggaatgg cgcggctggc gtattttgcc gctggtgtgc tacgacttac gttttccagt
    19021 gtggtcgcgc aatctcaacg attatgacct cgccctgtac gtcgccaact ggccagctcc
    19081 gcgctctctg cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt
    19141 ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg
    19201 ggtgattaat ccgcaaggcg agattatcgc taccgccgac gcgcataagg caacgcgtat
    19261 tgatgcggag ctgtcgatgg tggcattgcg ggaatataga gaaaagtttc cggcatggcg
    19321 ggatgcggat gagtttaggt tgtggtgagt gggggttgtg tttagggccg ggggctgggc
    19381 tagattacga aagcccctaa cttaaacaca atattacgcc agaagtcacc atttccaaaa
    19441 cacagcgtcg ctgtaccgtg ggttatctgc ccaaccgcag cgacacgccg gtgatcaaaa
    19501 tcagcgccaa atggctgaga aaagcgggat ttgagatcgg cacgggcgtt accgtgaaga
    19561 tctcggaggg atgtttgatc ctgcttggga taacaacgaa gtacaggaac tgcgcaggga
    19621 actttatcag gttaaacagc cagttaaggg ga
//
LOCUS       DADLCQ010000078        19375 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000078 DADLCQ010000000
VERSION     DADLCQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.078"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="JQG95_002555"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="JQG95_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY5889067.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="JQG95_002556"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="JQG95_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY5889068.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDAQVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQG95_002557"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="JQG95_002557"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY5889069.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="JQG95_002558"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="JQG95_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY5889070.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="JQG95_002559"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="JQG95_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY5889071.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="JQG95_002560"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="JQG95_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY5889072.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="JQG95_002561"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="JQG95_002561"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY5889073.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="JQG95_002562"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="JQG95_002562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY5889074.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="JQG95_002563"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="JQG95_002563"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY5889075.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="JQG95_002564"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="JQG95_002564"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY5889076.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="JQG95_002565"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="JQG95_002565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY5889077.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="JQG95_002566"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="JQG95_002566"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY5889078.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="JQG95_002567"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="JQG95_002567"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY5889079.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="JQG95_002568"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="JQG95_002568"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY5889080.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="JQG95_002569"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="JQG95_002569"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY5889081.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="JQG95_002570"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="JQG95_002570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY5889082.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="JQG95_002571"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="JQG95_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY5889083.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="JQG95_002572"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="JQG95_002572"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY5889084.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4768 a         4858 c         5122 g         4627 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccaagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgctcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggcg caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaagtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgctgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctgg cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccg
//
LOCUS       DADLCQ010000079        19295 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000079 DADLCQ010000000
VERSION     DADLCQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.079"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..2082
                     /gene="pbpC"
                     /locus_tag="JQG95_002573"
     CDS             <1..2082
                     /gene="pbpC"
                     /locus_tag="JQG95_002573"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY5889085.1"
                     /translation="EALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTM
                     QVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAASWA
                     YLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQV
                     KESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRL
                     PPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLAL
                     DEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGPK
                     RFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPD
                     DPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGVN
                     ARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGVI
                     CWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVAA
                     DCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGA
                     IIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDV
                     GQIATVKFVMQ"
     gene            2232..2663
                     /gene="ndk"
                     /locus_tag="JQG95_002574"
     CDS             2232..2663
                     /gene="ndk"
                     /locus_tag="JQG95_002574"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY5889086.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTC"
     gene            2813..3967
                     /gene="trmG/rlmN"
                     /locus_tag="JQG95_002575"
     CDS             2813..3967
                     /gene="trmG/rlmN"
                     /locus_tag="JQG95_002575"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY5889087.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4252..5265
                     /gene="rodZ"
                     /locus_tag="JQG95_002576"
     CDS             4252..5265
                     /gene="rodZ"
                     /locus_tag="JQG95_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY5889088.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGVWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5292..6419
                     /gene="ispG"
                     /locus_tag="JQG95_002577"
                     /gene_synonym="gcpE"
     CDS             5292..6419
                     /gene="ispG"
                     /locus_tag="JQG95_002577"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY5889089.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="JQG95_002578"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="JQG95_002578"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY5889090.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /locus_tag="JQG95_002579"
     CDS             7814..8434
                     /locus_tag="JQG95_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY5889091.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="JQG95_002580"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="JQG95_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY5889092.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="JQG95_002581"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="JQG95_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY5889093.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="JQG95_002582"
     CDS             11283..11498
                     /locus_tag="JQG95_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889094.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JQG95_002583"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JQG95_002583"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY5889095.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="JQG95_002584"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="JQG95_002584"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY5889096.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="JQG95_002585"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="JQG95_002585"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY5889097.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="JQG95_002586"
     CDS             complement(16234..16773)
                     /locus_tag="JQG95_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889098.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="JQG95_002587"
     CDS             complement(16789..17307)
                     /locus_tag="JQG95_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY5889099.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="JQG95_002588"
     CDS             complement(17618..17809)
                     /locus_tag="JQG95_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY5889100.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="JQG95_002589"
     CDS             17827..17979
                     /locus_tag="JQG95_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889101.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18161..>19295
                     /gene="pdeF"
                     /locus_tag="JQG95_002590"
     CDS             18161..>19295
                     /gene="pdeF"
                     /locus_tag="JQG95_002590"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY5889102.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4543 a         4696 c         5247 g         4809 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttacctg ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattgttg
     1441 aatcaggtca ataatatctt actgtcgcgc agtgcgaatc tgccggaaga cccgcgcccg
     1501 aactcagtca ctcgtggcgt tatctgctgg ccgggcggac agtctttgcc ggaaggtgac
     1561 ggtaactgtc gccgccgcct ggcaacctgg ctgctggatg gcagccagcc gccaacgctg
     1621 ttactgtcag agcaggaagg gattaatggc attcgcttcc ccatctggct ggatgacaaa
     1681 gggcagcgcg tcgccgctga ctgcccgcag gcgaagcaag aaatggtaaa tgtctggccg
     1741 ctaccgctgg aaccctggct gcccgcatca gaacgtcgcg ctgtgcgctt accaccggcc
     1801 tcgacaattt gcccgccata cggtcacgat gcccagctcc ctctgcagct gacaggcgtt
     1861 cgcgatggcg cgattattaa acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa
     1921 tcaagtggag gggcaggtga acgctggtgg tttctgaatg gcgaaccgtt aactgaacgc
     1981 gggcgcaacg tgaccctgca tttgacggat aaaggtgatt atcaattgct ggtgatggat
     2041 gatgtggggc aaatcgcgac agtgaaattt gtcatgcaat agtctgtttt gtccctgatt
     2101 gttgctaaaa ctcattttat tttaaaaaaa atgttacctt catcaatagt caacggccct
     2161 gttgctcatt ataatccgcg ccatctcgta cgctggtaca gacaacaaca gaacaattta
     2221 cagaggtaaa aatggctatt gaacgtactt tttccatcat caaaccgaac gcggtagcaa
     2281 aaaacgtcat tggtaatatc tttgcgcgct ttgaagctgc agggttcaaa attgtcggca
     2341 ccaaaatgct gcatctgacc gttgaacagg cacgtggctt ttatgctgaa cacgatggaa
     2401 aaccgttctt tgatggtctg gttgaattca tgacctctgg cccgatcgtg gtttccgtgc
     2461 tggaaggtga aaacgccgtt cagcgtcacc gcgatctgct gggcgcgacc aatccggcaa
     2521 acgcactggc tggtactctg cgcgctgatt acgctgacag cctgaccgaa aacggtaccc
     2581 acggttctga ttccgtcgaa tctgccgctc gcgaaatcgc ttatttcttc ggcgaaggcg
     2641 aagtgtgccc gcgcacctgt taataatttc gtaaatgccg cgtgcaaacg tggcatccgt
     2701 gcgccagaat ttgtacaatg cagcgccccc ggacgagcag ccgctcaccg gggcgtttct
     2761 tttttcaacc ctccaggggc cataacgtgt aataacgagg ccggaatagc atatgtctga
     2821 acaattagtc acacctgaaa acgtcaccac gaaagatgga aaaatcaacc tgctggatct
     2881 caaccgtcag cagatgcggg agttttttaa agatttaggt gaaaaaacct tccgcgccga
     2941 tcaggtgatg aagtggatgt atcactattg ctgcgacaac tttgatgaga tgaccgacat
     3001 caacaaagtg ttgcgcggca aactgaaaga ggtggcggaa atccgcgcac cggaagtggt
     3061 tgaagaacag cgttcatccg acggcaccat taaatgggcg atcgccgttg gcgatcagcg
     3121 cgtcgaaacg gtgtatatcc cggaagacga ccgtgccacg ctctgcgtct cttcgcaggt
     3181 ggggtgtgcg ctggagtgta aattctgttc caccgcccag cagggcttta accgcaacct
     3241 gcgggtatcg gaaattattg gccaggtgtg gcgtgcggcg aaaatcgtcg gcgcagcaaa
     3301 agtcaccggt cagcgcccaa tcactaacgt cgtgatgatg ggcatgggcg agccgctgct
     3361 caacctgaac aacgtcgttc cggcgatgga aatcatgctt gatgatttcg gttttggcct
     3421 gtctaaacgt cgcgtgacgc tttccacttc cggtgtagtt ccggcgctgg ataaactggg
     3481 cgatatgatc gacgttgcac tggcaatttc cctgcacgcg ccgaacgacg aaattcgtga
     3541 cgaaattgtg ccgatcaaca aaaagtacaa tatcgaaacg ttccttgctg cggtgcgccg
     3601 ttatctggag aaatccaacg ccaatcaggg ccgagtcact attgaatacg tgatgcttga
     3661 ccacgttaac gacggcactg aacacgcgca ccaactggcg gaactgctga aagatacgcc
     3721 gtgtaagatc aacctgatcc cgtggaaccc gttcccggac gcgccgtatg gacgcagttc
     3781 gaacagccgt atcgaccgtt tctcaaaagt gctgatgagc tacggtttca ccactattgt
     3841 gcgtaaaacg cgtggtgatg atatcgatgc tgcctgtggt cagttggcgg gcgatgttat
     3901 cgaccgtacg aaacgtactc tgcgtaaacg gatgcagggt gaagccatcg atattaaagc
     3961 ggtctgatag ccattacgcc acggttactt tatgtaccgt ggcgtaatct tttatgaatg
     4021 aagtattaag ttgcaattgt ctggtcaata ttaacggtgc gtttttgctg actttaaggc
     4081 agtatgtaac gatgcaacag taagttagcc tcagtgaatg tgggctttgt cacgagcaca
     4141 cagacggtct tatactgtat gataacggtt aacttaacgg atgtttcgcg gtgtgggtga
     4201 gcatgatggt tcaccggcat ctcaattctc atttaaacgt acctgcagcg aatgaatact
     4261 gaagccacgc acgaccaaaa tgaagcactt actaccggcg ctcgcctgcg taatgctcgc
     4321 gaacaactag gacttagtca gcaggccgtt gccgagcgac tttgcctgaa ggtttccacg
     4381 gtacgcgaca ttgaagaaga taaggcaccc gccgatcttg cttcaacatt cctgcgcgga
     4441 tatatccgct cttatgcgcg tctggtacat attccagaag aagaactgct gccagggctg
     4501 gaaaagcagg ctccacttcg ggctgcaaaa gttgcgccaa tgcagagttt ttccctcggt
     4561 aaacgccgca aaaaacgcga cggctggcta atgaccttca cctggctggt gttgtttgtg
     4621 gttatcggcc tgagcggtgt ctggtggtgg caagaccaca aagctcagca ggaagagatc
     4681 accactatgg ccgatcaatc ttcggcggaa ctgagcagta atagcgagca ggggcagagt
     4741 gttccgttaa atacgtcgac aactacagac ccggctacaa cgtccacgcc gccagcgtct
     4801 gtggatacta ccgcaaccaa cacgcaaaca cctgccgtaa ctgcgccagc accagctgtt
     4861 gatccgcaac agaatgcggt tgtttcgcct tcgcaggcaa atgttgatac cgccgcgacc
     4921 ccggtaccaa cggcagcaac aacgccagat ggtgctgcgc cgttgccaac cgatcaggct
     4981 ggcgtgacca cgccggcggc tgatccgaat gcgctggtga tgaactttac tgccgattgc
     5041 tggctggagg tcactgatgc taccggtaaa aaattgttta gcgggatgca gcgtaaagac
     5101 ggtaatttga atttaaccgg ccaggcaccg tataaactga aaattggtgc gccagccgca
     5161 gtacagatcc agtatcaagg gaaacctgtc gatctgagtc gttttatcag aactaaccag
     5221 gttgcgcgtc tgaccctcaa tgccgaacaa tcaccggcgc agtaacagac gggtaacgcg
     5281 ggagattttt catgcataac caggctccaa ttcaacgtag aaaatcaaca cgtatttacg
     5341 ttgggaatgt gccgattggc gatggtgctc ccatcgccgt acagtccatg accaatacgc
     5401 gtacgacaga tgtcgaagca acggtcaatc aaatcaaggc gctggaacgc gttggcgctg
     5461 atatcgtccg tgtctccgta ccgacgatgg acgcggcaga agcgttcaaa ctcatcaaac
     5521 agcgggttaa cgtgccgctg gtggctgaca tccacttcga ctatcgcatt gcgctgaaag
     5581 tagcggaata cggcgtcgat tgtctgcgta ttaaccctgg taatatcggt aatgaagagc
     5641 gtattcgcat ggtggttgac tgtgcgcgcg ataaaaacat tccgatccgt attggcgtta
     5701 acgccggatc gctggaaaaa gatctgcaag aaaagtatgg cgaaccgacg ccgcaggcgt
     5761 tgctggaatc cgccatgcgt catgttgatc atctcgatcg cctgaacttc gatcagttca
     5821 aagtcagcgt gaaagcgtct gacgtcttcc tcgctgttga gtcttatcgt ttgctggcaa
     5881 aacagatcga tcagccgctg catctgggga tcaccgaagc cggtggtgca cgcagcgggg
     5941 cagtaaaatc cgccattggt ttaggtctgc tgctgtcgga aggcatcggc gacacgctgc
     6001 gtgtatcgct ggcggccgat ccggtcgaag agatcaaagt cggtttcgat attttgaaat
     6061 cgctgcgtat ccgttcgcga gggattaact tcatcgcctg cccgacctgt tcgcgtcagg
     6121 aatttgatgt tatcggtacg gttaacgcgc tggagcaacg cctggaagat atcatcactc
     6181 cgatggacgt ttcgattatc ggctgcgtgg tgaatggccc aggtgaggcg ctggtttcta
     6241 cactcggcgt caccggcggc aacaagaaaa gcggcctcta tgaagatggc gtgcgcaaag
     6301 accgtctgga caacaacgat atgatcgacc agctggaagc acgcattcgt gcgaaagcca
     6361 gtcagctgga cgaagcgcgt cgaattgacg ttcagcaggt tggaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccactgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttgggggg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgact tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 aagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggt aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataaccc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgttttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgtgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agtaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgattt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcgga attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg ctgaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aagcggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgctgccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atgcaactta
    18181 tataaaaata cgtgataaat ggtgggggct tccgctgttc ctgccttctt taatcttgcc
    18241 cattttcgcc cacattaata ctttcgcgca tatttcttcc ggtgaggttt ttctctttta
    18301 tctgcctttg gcactgatga tcagcatgat gatgtttttc agctgggcgg cattgccagg
    18361 gatcacctta gggatttttg tccgcaaata tgcagagctg ggtttttacg aaacgctctc
    18421 attaacggct aattttatta tcattatcat tctctgttgg ggcggttaca gggtctttac
    18481 tccccggcgt aacaacgttt cacatggtga tacccgttta atttcccagc gtatattctg
    18541 gcagatagtg tttcctgcaa cgctgtttct gatacttttc cagtttgctg catttgtagg
    18601 attactggcg agcagagaaa atctggtcgg tgtcatgcct tttaacctcg ggactttaat
    18661 caattatcag gccttactgg tgggtaatct gatcggtgtc ccgctgtgct acttcatcat
    18721 tcgggtagtg cgaaatccat tttatttacg tagctattat tcgcaattaa aacagcaggt
    18781 tgatgccaaa gtcaccaaaa aagagttcgc gctctggcta ctggcattag gtgctttatt
    18841 gttgctgtta tgcatgccgt taaatgaaaa aagcacaatt tttagcacca attatacctt
    18901 gtcattattg ctgcccctga tgatgtgggg agcaatgcgc tatggttata agctgatttc
    18961 gctgctctgg gcggtcgtgt tgatgatcag catccacagc tatcaaaatt acattcccat
    19021 ttatcctggc tataccacgc agctgaccat aacctcctcc agttatctgg tattctcttt
    19081 tattgtcaat tatatggctg tactggcaac tcgtcagcga gcggtagtca gacgcattca
    19141 gcggcttgcg tatgtggacc cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc
    19201 gttgcgtgat gccccctggt ctgcgctttg ttatttacgc atccctggca tggaaatgct
    19261 ggttaagaac tatggcatca tgctgcggat tcaat
//
LOCUS       DADLCQ010000080        19261 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000080 DADLCQ010000000
VERSION     DADLCQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.080"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            32..331
                     /locus_tag="JQG95_002591"
     CDS             32..331
                     /locus_tag="JQG95_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY5889103.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            328..2076
                     /locus_tag="JQG95_002592"
     CDS             328..2076
                     /locus_tag="JQG95_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY5889104.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPAEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            2365..2643
                     /locus_tag="JQG95_002593"
     CDS             2365..2643
                     /locus_tag="JQG95_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889105.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            2640..2945
                     /locus_tag="JQG95_002594"
     CDS             2640..2945
                     /locus_tag="JQG95_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889106.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            2955..3116
                     /locus_tag="JQG95_002595"
     CDS             2955..3116
                     /locus_tag="JQG95_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY5889107.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            3180..3482
                     /locus_tag="JQG95_002596"
     CDS             3180..3482
                     /locus_tag="JQG95_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889108.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            3783..4940
                     /locus_tag="JQG95_002597"
                     /pseudo
     CDS             3783..4940
                     /locus_tag="JQG95_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            4980..5552
                     /locus_tag="JQG95_002598"
     CDS             4980..5552
                     /locus_tag="JQG95_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY5889109.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            5554..6765
                     /locus_tag="JQG95_002599"
     CDS             5554..6765
                     /locus_tag="JQG95_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY5889110.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            6762..7100
                     /locus_tag="JQG95_002600"
     CDS             6762..7100
                     /locus_tag="JQG95_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY5889111.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            7097..7393
                     /locus_tag="JQG95_002601"
     CDS             7097..7393
                     /locus_tag="JQG95_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY5889112.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            7393..7833
                     /locus_tag="JQG95_002602"
     CDS             7393..7833
                     /locus_tag="JQG95_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY5889113.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            7817..7999
                     /locus_tag="JQG95_002603"
     CDS             7817..7999
                     /locus_tag="JQG95_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889114.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            8055..8345
                     /locus_tag="JQG95_002604"
     CDS             8055..8345
                     /locus_tag="JQG95_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY5889115.1"
                     /translation="MDDIAVKQWREKSRQLAEQGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPP"
     gene            8365..10026
                     /locus_tag="JQG95_002605"
     CDS             8365..10026
                     /locus_tag="JQG95_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY5889116.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            10040..10321
                     /locus_tag="JQG95_002606"
     CDS             10040..10321
                     /locus_tag="JQG95_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889117.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="JQG95_002607"
     CDS             complement(11118..12578)
                     /gene="phoQ"
                     /locus_tag="JQG95_002607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY5889118.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="JQG95_002608"
     CDS             complement(12578..13249)
                     /gene="phoP"
                     /locus_tag="JQG95_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY5889119.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13418..14788)
                     /gene="purB"
                     /locus_tag="JQG95_002609"
     CDS             complement(13418..14788)
                     /gene="purB"
                     /locus_tag="JQG95_002609"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY5889120.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="JQG95_002610"
     CDS             complement(14792..15433)
                     /gene="hflD"
                     /locus_tag="JQG95_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY5889121.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="JQG95_002611"
     CDS             complement(15469..16575)
                     /gene="mnmA"
                     /locus_tag="JQG95_002611"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY5889122.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="JQG95_002612"
     CDS             complement(16629..17090)
                     /gene="nudJ"
                     /locus_tag="JQG95_002612"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY5889123.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="JQG95_002613"
     CDS             complement(17100..17753)
                     /gene="rluE"
                     /locus_tag="JQG95_002613"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY5889124.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            17925..19175
                     /gene="icd"
                     /locus_tag="JQG95_002614"
     CDS             17925..19175
                     /gene="icd"
                     /locus_tag="JQG95_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY5889125.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5016 a         4728 c         5158 g         4359 t
ORIGIN      
        1 ataaagaaca acacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc
       61 cagtggcaac actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg
      121 catggtaacg attaaaggcg tggcagaacg gcgcatcacc taccgccgtg aaggttacga
      181 atatgattgc gtgatgcctg tttatcaatt ccggcgtgat ttcacgctgg tacaggcagc
      241 gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg aaataaaaaa
      301 gatgcttaac gtattcaggg gtaaaaaatg aaactggcac cgaacgtaaa aaaacagcca
      361 cgcggaataa aacacaaaga cacagaggta attatctttg cgggtagtga tgcctggtcg
      421 cacgcgaaac aatggcagga gcaggatggc cccgcatccg gcgataatgt gccgcctgtg
      481 tggcttgggc caaatcagct tgccgaactt gatgcactga aaattgttcc ggatggaaaa
      541 aaacgcgtaa ggctgtacca ggccggagaa ctggatttgg tggagaccaa aaagattggt
      601 cagaagctgg cggcggcaga tattcaggac gcaaattttt accccgaagg aatgcacgtc
      661 cagaagtgtg aaaactggcg gcgctatctg aatgctgagc gtgaaaatat tgccgcaggg
      721 cttaccatgc cggagcagaa aaatacgcaa ctggcacaaa tggcagacag tgagcgcgcg
      781 cagctgcttg ccgagcgctt tgatggcgtt tgtgtgcatc aggaaagtga aatcgttcac
      841 gtatggcgcg gcggggtatg gtgtccggtc agcacaatgg aacttagccg cgaaatggtg
      901 gcgatctatt cagagcacag ggccactttc agcaagcgcg taatcaataa cgccgtggaa
      961 gcgttaaaag ttattgccga acccatgggc gagccgtccg gcgatttgct gccgttcgcc
     1021 aatggtgcgc ttgacctgaa aacgggggaa ttttccccgc acacgccgga gaactggatc
     1081 accacgcaca acggcattga gtacacgcca ccagcaccag gggagaacat ccgcgataac
     1141 gcgctaaact ttcataaatg gcttgagcac gcagccggaa aagaccagcg caagatgatg
     1201 cgtatatgtg ccgcgctgta catgattatg gcgaaccggt acgactggca gatgtttatt
     1261 gaggccaccg gagacggcgg gagcggtaaa agtacattca cacacatagc cagccttctg
     1321 gcagggaaac agaacacggt aagcgctgaa atgacatcgc ttgatgatgc tggtggacgt
     1381 gcgcaggttg tcgggagtcg tcttatcgtc ctggctgacc agccgaaata tacaggcgaa
     1441 ggaacgggca tcaagaaaat cacgggcggt gaccccgcgg aaattaaccc gaaatacgaa
     1501 aagcgtttca cggctgtaat cagggcggtg gtgctggcga ccaacaataa cccgatgata
     1561 ttcaccgaac gggccggagg tgtggcacgt cgtcgcgtga ttttccgttt cgacaatatc
     1621 gttagtgagg ccgaaaaaga cagggagcta ccggaaaaga ttgcggctga aatccctgtc
     1681 attatccgcc gattgctggc gaactttacc gactctgaga aagcacgggc tttactactg
     1741 gaacagcgtg acggtgatga agctctggca ataaagcaac agacggatcc ggttattgag
     1801 ttttgccagt tcctgaattt tctggaggaa gcacgcggcc tgatgatggg cggcggtggc
     1861 gattcagtga agtacacgac cagaaacagc ctttaccgcg tctatctggc gtttatggcg
     1921 tacgcaggca ggagcaaacc gctaaacgta aatgactttg gcaaggctat gaagccagcc
     1981 gcgaaagttt acggacatga atatattacg cggaaagtta agggagtaac gcagactaac
     2041 gcaataacaa cagacgagtg cgacgcgttt ttataatttt ttgtaattgc tgtctaccct
     2101 gtctacctga gtaaagaaaa atacatttaa ttcagtgcat taagttaggt agatagcctt
     2161 tttttactgt ctacccacta tctaccctct ctacctgatt ttacctgaat cagacaggga
     2221 ggtagacacg gggtagatag tggataaaag cactctaccc cgctgaaagc agcgccatta
     2281 ctggcatgag ggccactaag gtagataagg tagacaaggg gaggcacaac tcaaaacttt
     2341 ttaaacgagg gggtaaaaat aaatatgcac acttcaggga aatttaataa atcattcaaa
     2401 aaacgcagag acagaacaga accgaaatat cgcgcgttag acatgacaga gcacgcttta
     2461 aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa agcacatccc
     2521 gaactgataa gcgcattcat gaccacgacg gcggcaaatt ttgccacgct gacagagcgg
     2581 gagattgccg aagcggaaca ggtaacaaca atcaacgtta aaaccggaga ggtggaatca
     2641 tgagcgaacc aaaatttggt gaaaaatttt acaagcacaa cggacggatc acgattctgc
     2701 aaataagcgc agctacacca ggctggtggg ttgaaacaga cgaaggatcc tcacccgtgg
     2761 catcatgggc gctttgtgtg cttagttacc ctgaccgcga tgtgtatcag gatattctgc
     2821 ctgtgatatc cactgacaaa ggcatgaaac ccgtagacat caaaaacatg gggtttcagt
     2881 gcgtaatgct cactgaaaaa atgatggaag agatgaaaaa gaacaatgcc ggatcgcttc
     2941 actgaggtgg caatatgaca gcacaaattg cagcttacgg gcggctggtg ggcgacccgc
     3001 tggtaaaaca gagcagcaag ggcacaccgg agccgccaac ggagaactac gcagacctga
     3061 gaaatgcagt aaatcgcact tacgcagaca tggggctgag caaattagca gagtaggaat
     3121 atcagaacca caccacgcag ccggagcaat ccggcttttt ttgcgtcatt tgaaacggta
     3181 tggagcacta ctggcttttc gtcacggtcc ggcatagtta ctatctgaaa caaacagaca
     3241 caacagagga aaacgcaatg ccgatgaaat ttgatgagat acttaaacag cgtgataaat
     3301 accatgctga caacatggag acgatgaata tcactgatta ccgcgcattt ctggaaacgg
     3361 gggcgctgat tgaaaaggat catcatggtt ttgttcgttg cgcgctatcc ggtgaaatgc
     3421 tggcagtaaa tcctgaacag acagatgcat tgatagaatt tctgaaaggg atcagagact
     3481 gagcacccat acagcacata cagccggagc aatccggctt tttgttattt tttgtaaatt
     3541 atttgttcgt ggttgttcca cgttgttcac tgagcggatc ggcatatttt acccgaactg
     3601 aatcatgatt attctcgccc gtggtgccag gacgctgggg ccactttccc gcctgttaat
     3661 gtgctcgcca atattcatta ccaggcggga aaacgatcgg tgcgattact gatttcctta
     3721 tgaaaaacgg ttgagtgttt gccgcgtcct ggagttcctt acttaacccc aggacttttt
     3781 ttatgcagag aataatcgaa ttacgccagc agaaaaccgc cattaaaaat cagatgcgcg
     3841 acatgctgga gaacgctgaa aaagaaaacc gcagtcttaa cgatgctgag ggcacaaaat
     3901 ttgacgaatt acgcgctaaa gctgaatccc tcgataaaga catttcccgc cttgaagcca
     3961 ttgcagacga agagcgcagc aagccaggta aaagcagcca gaccactgac cccgcagaac
     4021 tacgcaacta cattctgacc ggtgaaaccc gcgcattaag tacaggcgtt cccgctgatg
     4081 gtggttatac cgttatccct gaactgaaca ccgaaatcat gcgaatgctg gcggatgaat
     4141 ccaccatgcg tcgcatctgt accgtgaaga aaatcagcag caacgagttt aagcagcttg
     4201 tttccgctgg cggtgcgacc gttaaccacg gtgaagaggg taaggcacgc gaacagacca
     4261 gcaccccgca gattaacgag gtgagcatta agctgtatcc ggtctatgcg tacccgcgca
     4321 ccacacagga aatcgtggat ttttccgatg tggacatcct ttcatggctg acgggtgaga
     4381 ttggcgacac cttcacggaa accgaagaaa gcgatctggt tgtgggcgac ggtgacaaaa
     4441 aagcaaaagg ttttttatcc gtaccccgtg cagagaagaa cgacaaagag cgtgattttg
     4501 gtacgttgca ggtaattaaa ccttccgaat ctctggcgtg gacatctgcg gacccgctga
     4561 tcgacctgaa atttgcatta cgtaaaaaat accgcaaaaa cgcggtctga gtggttaact
     4621 ccacgacggc ggcaaaactt cagaaggtga agaacgcgaa cggtgattat atctggcgcg
     4681 accgtttaca gacgggtgat cctgatacgt tgctgggcct tccggtcgaa tatctggagt
     4741 ttatgcctga taacgttatt gccctgggtg acttcaaacg cggttactac attgttgatc
     4801 acgaaacagg tgttcgcacc agaccggaca acctgacaga accaggattc atcaaaattt
     4861 tcacgcaaaa atatttaggc ggtggcgtgg tggattcgaa cgcgatcaag attctggcac
     4921 tgccggaaga cgacgattaa cagcatacag aaggggctaa aaagcccctt tagtgtttta
     4981 tgggtgaaaa aattatgaag agtatggaaa tccggtcatc ggaaatcacc accagcggag
     5041 ccggtacgct gacgggctac gttgttcgct gggataagct ttcagaactg ttatggggtg
     5101 agttttacga aaaattccag cggggggcgt ttactgaatg gcttgcggcg ggtaatgacg
     5161 ttcgcggcct gtatgagcat gaccacagca tgttactggg gcgcacccgt tccggcacgc
     5221 tgaaactgga agaggacgaa acagggttac gctttgaact gaccccaccg gataccagta
     5281 cagggcgcga cgttatcgaa ctggttaagc gcggggacat ctcggggatg agctttggct
     5341 ttcgctcccg taaggatgta tgggatacca caacagatcc atgcgtgcgc accgtgctgg
     5401 tggcggaact gtacgaaatt accgttacat cggtaccggc ttaccccgat tccggcgtgg
     5461 agctggcccg ccgttccctg tatgagcagc accccgaaaa aatgccgcgt gcggataatc
     5521 gccgctggtg ggcggattta gcgggggtgt gatatgtggc ctttcagacg aaaaaaagag
     5581 cagcgcagca tgacgcttga tgaatttatg gcgctggccg gcacatcgaa cacgggggcg
     5641 ggtgagtacg tatcatctgg gacagcggaa tcactccccg ccgtcatgaa cgccgtcacg
     5701 gtcatctctg acgcggtggc taccatgccg tgttacctgt acctggtacg caatgagaag
     5761 gggaaggagg cccgcgaatg gcttgattct catccggtcg atcacatcct caacgagcgc
     5821 ccgaacgcgt ggcagacacc ctaccagttt aagcgaatga tgatccgcta ctgcctgtta
     5881 agtggtaatg cttatgcggt tattcagtgg gggcgtgatg gttttccggc ggctttacat
     5941 ccttacccgc cgcagtcggt gaacgtggag cagacaggtg atcataactg gcgctactgc
     6001 atcactgacg cctacaccgg aaacacccgc aactatttac cgtgggaggt tctccacctt
     6061 cgttactcca cggatgacgg ttttatgggg cgctcacctg taaccatctg ccgcgaatcg
     6121 ctggggcttg ggctggccca acaacgccac ggcgcgagcg tgatgcgtga tggcatgatg
     6181 gcggcagggg ttatcacgtc aggcgaatgg ctggacggcg tgaagggcaa acaggcatta
     6241 gccgcactgg aacgctacaa aggggccaga aacgccggaa aaacgcccat ccttgaaggg
     6301 gggatgagct atcagcagct gggcatgagt aatcaggatg ccgaatggct ggcctcccgt
     6361 cgcttcacca ttgaagacat cgcccgaatg ttcaacgtct cgccgatttt tttgcaggaa
     6421 tacagcaaca gcacctacag caacttcagc gaggcaagcc gcgcatttct caccatgacg
     6481 atgcgcccgt ggctggcgaa ctttgagcag cagataaaaa acgccttgct ggtggcctcg
     6541 cctgtacctg gtatccggta tcaggtggag tttgacagcg cggacctgtt acgggccaca
     6601 cctggcgaac gctttgccac ctatgagcgc ggcataaaat ccgacgttat gtgtccgaac
     6661 gaagcccgcg aacgtgaagg gctgtcaccg cgtgatggtg gtgatgagtt cagccaggca
     6721 tggaaacagg aagtaaaaat cagcgaggga gaaaaaccgg aatgaacata gggcgactgc
     6781 gtgacaggat aacgattcag accctgaaac agaccaggga tataaccggc gaaatactcg
     6841 aaacgtggga ggacggtcac acactctggg caagcgtgaa catggtcagc agcaaggagg
     6901 ccatttcatc gggcgcagag ctggcgattg gtaccgtaag gatctggata cggtaccgga
     6961 aggacatcaa cgccaccagc cggataaagg tcagtacggg gccgctggcg gggcgtgtac
     7021 tgaatatcat cgggcagccg ctgccggatg ccgccaggac acgccttgaa attctttgtc
     7081 gtgagggcgc ggaaaaatga cagaagaact tatcaccctg gaagaagtga aactccattg
     7141 ccgcatcgat ggcgacgatg aagaccagtt aatcagcgga tacattgccg catcgcttga
     7201 ggcgtgccag atacacatag gcaggcgctt tgatgacggg ctggcgttca cgccagccat
     7261 aaagattggc tgcatgatgt ttatcgctca cctgtatgaa aatcgccaga ttgtcgcgga
     7321 taacgcaaaa acacgcgtac ccatgacgat aggcgcactc tggacggctt accgtgatgt
     7381 gggggggtac tgatgccgtg gcaaccatta agacgatgca cagaaccagg ctgtaacagg
     7441 cgcgttaaat ccggcaagtg tgaggagcac aggcgggcgg catggcgtgc agaggatgcc
     7501 agacgaggac accgccgcgc gcgtggatat tccagacagt gggacaaata ccgcgccatg
     7561 tatctgagta aaaacccgtt atgcgtgcga tgccttgaga aggggatata tacgcccgcc
     7621 gtggtggtgg atcacattat cccgattaat ggcggtgatg atgttctctt ctggcccgaa
     7681 tggaaccatc aaccattgtg ccaggcgtgt cataaccaga aaacgaaatg gcttgatccg
     7741 gcaacaaaaa gcaagcgtgc cgcaggtgga tttcgtgaag aggaagaacg ggctgctaac
     7801 cgcaataact ggatgtatga cgctgatgaa tgagcgggaa caaaaccgcc ttatccgtgg
     7861 acttataagg cagcgtgacg catggaagac acaggaaaca gggcataaag ataaagcgtc
     7921 aggacgcgca gaacgcatca cagcgaagcg attaaccgac cgtgaccgcg aggttatgga
     7981 atgtttccgc aatcgctgat gaggctgtat gacggggtgg ggggcgtttt caggacaaac
     8041 ccgaccccgc ctacctggat gatatcgccg tgaagcagtg gcgggaaaaa tcgcggcagc
     8101 ttgcggaaca gggagacctg acccccgccg actggagcaa tctggaactg tattgcgtca
     8161 actactccat ttaccggaaa gccgttgcag accttgcggc gcgcgggttc agcattgtta
     8221 acagtcaggg cggcgagagc agaaaccccg cattaagcgc aaaatccgac gctgaaagag
     8281 tgatgataaa aatggcctcc ttgctcggtt ttgacccgat aagccgccgt aaaaatccac
     8341 cgtaaacaga agaagaggac gagcttgacc gcctggaata agtacgcaga agacgtaaaa
     8401 acgggcaaaa ttccggcctg taaacggtta aaacaggctg ttaaacggta cttttcggac
     8461 cttaaaagcc ccctttacac gttcgatcgt gaggttgtgg agcggtttat tgccttttcc
     8521 agggtgtgcc cgcacgtaaa aggaccgatg cgtggcagac ccattgagct ggagccgtgg
     8581 cagcagttcg cctttgcgtg catcctcggc tttaaggtta aggccaccgg acggcgcaaa
     8641 tacacgagcg cctttatcga agtgccgcgc aaaaatgcga aatccacgac cgccgcgatt
     8701 ctggctaact ggtttctgat tatggagaac ggtcagcagg atatctacac cgcagcggtg
     8761 agccgtgacc aggcgcggat cgtgtttgat aatgcgcgtc agatgtgcct tttatcccga
     8821 ccgttacgca ggcgggtgaa tattcaggcg cataaggtga tacacccgaa aagcaacagc
     8881 ctgttaaagc cgctggcagc aaaagcggca accatcgagg ggacaaaccc cagtctttcc
     8941 atcgtggacg aataccacct acaccccgat aacggcgttt attccgcgct tgagctgggg
     9001 atgggcgcac gtcccgaagg gctgttattt gccattacca catcgggcag taacgtcgtt
     9061 tcagcctgta aacagcacta tgattattgc tgccagatcc tggacggcga agaggtcaac
     9121 gattcaattt ttgtactgat ttacgaactg gacgacgaaa acgaggttga tgatccggcg
     9181 atgtggataa aggcgaaccc taatattgat gtttccgtgg atcgtgaaaa actggcctca
     9241 accatccaga aagcgcgggg tattccgtcg cagtgggtgg agatgatgac aaagcgattt
     9301 aatatctggt gtcaggggac taccccgtgg atgggtaatg gtgcatgggc tgagtgtgcc
     9361 ggaacgttca cggaggaaga tttacacggt caggagtgtt acgcggggct ggacttatca
     9421 tcaaccagcg atatttccag cgtatgctat gcctttccgg tcggtaaaac cattatgctg
     9481 atttcccgtc attatctgcc ggagttccag ctacagaacc ccgccaataa aaaccgcgct
     9541 gtctatcgcc agtgggctaa agcgggctgg atacgcacaa cgccaggtga ctgcattgat
     9601 tacgaccgga tcagagatga catcatgcag gatgcggaga aatttaatat caggctggtg
     9661 ggctttgata cgtggaacgc cacgcatctc aggacgcaat tacagggggc tggttttgag
     9721 gtggagccgt tcccgcaaac ctacctcaga ttcagtccgg cggcgaaatc gttcgaagtt
     9781 tttgttaacc gcagggtgat tgtgcatcgt ggcgatccgg tgttgtcctg gtcgatgagt
     9841 aacgtcgtga tgcagagtga cgcgaacgcc aatatcaagc cgaacaagaa aaaatcaccg
     9901 aacaagatag acccgagcgt agcggcgctg atggcgtttg gtacattcca ggcagagcat
     9961 gaggattttg cttttgatat aagcgacagc caccgccaga aactggaaga atttagcggg
    10021 gtgtaatgag gtcagcagca tgacagaagc cgaaatactg cgattaatcc gccgcgtctc
    10081 tggaatcagc cagcaggctg acgaacagac cacgcagccg gacagcgtga cagccgaaaa
    10141 ttatgcgcgt gttgttgctg aggtgatgcg ccgtgatggt atccagctta atgatgcgga
    10201 tatgcgcgac atacggatcc gcgttctgga aatgctggcc tacaatcgcc gcgttgaact
    10261 gtatcgggag aaggagaaaa tcacgtacca ctggaagaag ccggagcggt tacggcggta
    10321 actcactgag attaaagcaa agcgcaaaat tgcgctggct acatccctca catctgagga
    10381 ctgttaacca gcaggccgga atatattcag aaattacgaa attttcgcag tttaattact
    10441 cgtcagctta atcgctaacc cgctctgata ttttcggaaa cctcaatttg aggaagtcgg
    10501 cgaggtaact gtttgatatt cccgatagcg caaaaatgcg cggtcgggca gaatgaccac
    10561 cacctcaatc gtgacggaga gtgacaacca gtgacacacc gtgacaaaaa gacggtttaa
    10621 ccgttcatta aaaatcgacg tgattttgtg ctttccggat gacgtggcca acatcatttt
    10681 ttagcgaaaa attctgatta aggtcactgg ctgaacggtc ataatgacca cgcagaccat
    10741 cgaggcaacc atagcgatca tcgttacggt tggagctttt ccgccattgg tggaaaaggt
    10801 gcacatctgg atcatcatta cggtgatcaa gggtatgtaa aacccaccag cctgattaaa
    10861 ggttaaccgg aaaaaaaatc cggtagcccc gttatgggga tccccatatc gacattaacg
    10921 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
    10981 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
    11041 tttgcgattt ttggcacttt ttcaatgaaa attgtttgat cgaatcaatg acttgatgta
    11101 gtggtaaaag gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    11161 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    11221 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    11281 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11341 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11401 tatttacagg cattatccag cacgttgccc atcacctcga caaaatcgtt ctgctcaccg
    11461 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11521 ttgttcagcg ctgaggtgag attgtccagc agtggggcga ccggatgcag ctcgcggctg
    11581 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11641 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ttcactacgc
    11701 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11761 gtgagcgtcg tacggtattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11821 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11881 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11941 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    12001 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    12061 aattttggca tccgcgatgt tgccgggtag acgtttaccg ccaccgagtg ggtcatctcc
    12121 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    12181 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    12241 cagtcaggct ggatcatttt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12301 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12361 tcgacatgca acttattgtt ttcccactgc gcaagggtat agaacagatt gctctcgcca
    12421 cgtaacagcc gaaacgtagt tttatcgaaa ctgacgctat aaccgatcag cgcgaccatt
    12481 ccgtaggcaa gcgaaagcac cagtactacc gctgccgttg ccaacagaaa acgtacccgc
    12541 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacaggtagc
    12601 cctggccgcg aacggtggta atgacttctt gcggatattg tgcctgaatt tttttgcgca
    12661 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc ggatagagtt
    12721 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12781 tggtgtattc gaacgcggtc agtttgatca cttcgtcatt aatagataat tcacggcgag
    12841 agagatcaac ctgaaacggg gggagcgaaa tgacctgtga agccagaccg ctattacgcc
    12901 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12961 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    13021 ccagaatcgg cagtgaaaca tcgttgctac gccagcggcg aatcagtgac agaccgtcct
    13081 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    13141 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    13201 taaggtggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    13261 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgt ttatgggggt aaacattaaa
    13321 taaaccagcg gggaggggag gtaaagtgaa aaaagaaaaa gtggataatc ttaataagca
    13381 ggccggacag catcgccatc cggcactgat ttgaggctta tttcagctca tcaaccatcg
    13441 tgatagctcg accaatatag ttagccggcg tcatcgcttt caggcgggct ttctcttctt
    13501 ctggcaacgc cagaccatcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13561 gagtaagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13621 ggattggttc tgccagcact tcccagttgt gatccagctc atccagcaga tggtcacggt
    13681 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13741 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13801 ccggcagttt gcttgccaga tgctgcaata ccgcgttgga aaggcccaga ttcccttcgg
    13861 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13921 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13981 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    14041 gttcaatctg ggtagtgtac gggttccatt gaatacccag cgaggtgacg aactcttcgc
    14101 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    14161 cgccgttgat tttgccgagg atctccacct ggttaagctg gcggtgctgg cgctccatac
    14221 ggtaggcgac gtttgccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    14281 tacgagacag cagcgggata tcgcggtact gaacggcgag atctttaatg ccatcaatca
    14341 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14401 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14461 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14521 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ttggcgacaa
    14581 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14641 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14701 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14761 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14821 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgtaga cgtccgccgc
    14881 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggttgcg cgaactttcg
    14941 cctgcacttg tgggctttgc agtacagcag gggaaccggt gacctgaatg cgcgggccaa
    15001 gcgggctgat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    15061 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    15121 cgcctttcgc tgaggagagt ttgcgctcaa gcaccatcaa gctgagtgtg tagcgggtta
    15181 attcggcgtt taagccctgg caactgctgg cattgagcac gccgagcagg gtttccagcc
    15241 cgacgcgcag gttggcttcg ctaccgccaa aaaccgccag cgtcgagctg gggttcatat
    15301 caataatact gttgagtgag acgtgtagcg catcggcatc acaatgcccc tggtgagcga
    15361 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15421 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15481 cggcagacgc tgctcaataa taccgccacc gaggcacact tcaccgttat agaagacggc
    15541 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcactttaa tgcgatcgtc
    15601 gtccagcgcc ttgacggtgc acggaatgtc ggtctggcga tagcgggttt ttaccgtgca
    15661 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcaaccc
    15721 gacagacatc agccgcgggt gttcatggcc ctgagcgaca accagaatgt tgttttcgac
    15781 gtctttgtcc accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15841 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15901 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15961 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    16021 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgcctaccg ggaacaggct
    16081 ttgcgcaatc tgctcatggc tgagcgtata aaggaagtag ctctggtctt tattgctgtc
    16141 cagaccacgc agcaggcggc tcttgccatc gacatcggcc cgacgtacgt aatgaccggt
    16201 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    16261 ctccttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16321 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16381 gagcttgtcg cagaccgcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16441 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16501 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16561 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16621 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16681 ggataacgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16741 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16801 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16861 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16921 ccggtttctt cccacagttc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16981 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    17041 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcatcct
    17101 taatctgtca cttctcgcca ttcgccattg gcaagattat ccaaagagta atctcccatc
    17161 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    17221 cgattacgtc cttcatataa ggtgatcttc agccagctgg tgggaatact tttacgttca
    17281 cgaattggtg gattccgcgg ccataaccac gcgggttcgt caaccagctc cgcgcctgcg
    17341 ggcagggtag ggccatcatt taaggttacg ccattgcgca aggcttcaag tgcgtcttgt
    17401 gtgggaatac cttccacctg cacataatag atttttccgg tgcgtttacc cggctgggtt
    17461 aaacgcgcct gcaacgcgcc gttattggtc agcaccagca acccttcgct atcgcggtca
    17521 aggcgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17581 gtttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaagaggat cacacgcgtg
    17641 ggctggtttt catgtttacg cctggtagaa cgttgtgagc tgaatcgctt aacctggtga
    17701 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17761 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag tcaattacaa
    17821 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17881 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17941 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    18001 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    18061 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    18121 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctgcctg
    18181 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat taaaggcccg ctgaccactc
    18241 cggttggtgg cggtattcgt tctctcaacg ttgccctgcg tcaggaactg gatctctaca
    18301 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18361 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18421 aagctgactc tgccgacgcc gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18481 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18541 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18601 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18661 accagctggc gcgtgaagaa tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18721 ttaaaaaccc gaataccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18781 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18841 gtgactacat ttctgacgct ctggcagcgc aggttggcgg tatcggtatc gcccctggag
    18901 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatacg
    18961 ccggtcagga caaagtaaac cctggctcta ttattctctc cgctgagatg atgttacgcc
    19021 atatgggctg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    19081 ccaagaccgt aacctatgac ttcgagcgtc tgatggaagg cgctaaactg ctgaaatgtt
    19141 cagagtttgg tgatgcgatc atcaagaata tgtaatcact acatgtgtta aatatttaaa
    19201 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atatcgagca
    19261 t
//
LOCUS       DADLCQ010000081        19199 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000081 DADLCQ010000000
VERSION     DADLCQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.081"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..721)
                     /gene="glyQ"
                     /locus_tag="JQG95_002615"
     CDS             complement(<1..721)
                     /gene="glyQ"
                     /locus_tag="JQG95_002615"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY5889126.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPL"
     gene            complement(816..1115)
                     /locus_tag="JQG95_002616"
     CDS             complement(816..1115)
                     /locus_tag="JQG95_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889127.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            1290..2285
                     /locus_tag="JQG95_002617"
     CDS             1290..2285
                     /locus_tag="JQG95_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY5889128.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(2327..2764)
                     /locus_tag="JQG95_002618"
     CDS             complement(2327..2764)
                     /locus_tag="JQG95_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY5889129.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(2810..3151)
                     /locus_tag="JQG95_002619"
     CDS             complement(2810..3151)
                     /locus_tag="JQG95_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889130.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(3320..4774)
                     /gene="xylB"
                     /locus_tag="JQG95_002620"
     CDS             complement(3320..4774)
                     /gene="xylB"
                     /locus_tag="JQG95_002620"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY5889131.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(4846..6168)
                     /gene="xylA"
                     /locus_tag="JQG95_002621"
                     /pseudo
     CDS             complement(4846..6168)
                     /gene="xylA"
                     /locus_tag="JQG95_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            6534..7526
                     /gene="xylF"
                     /locus_tag="JQG95_002622"
     CDS             6534..7526
                     /gene="xylF"
                     /locus_tag="JQG95_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5889132.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            7604..9145
                     /gene="xylG"
                     /locus_tag="JQG95_002623"
     CDS             7604..9145
                     /gene="xylG"
                     /locus_tag="JQG95_002623"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY5889133.1"
                     /translation="MSYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            9123..10304
                     /gene="xylH"
                     /locus_tag="JQG95_002624"
     CDS             9123..10304
                     /gene="xylH"
                     /locus_tag="JQG95_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY5889134.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            10382..10911
                     /locus_tag="JQG95_002625"
                     /pseudo
     CDS             10382..10911
                     /locus_tag="JQG95_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            11023..11559
                     /locus_tag="JQG95_002626"
     CDS             11023..11559
                     /locus_tag="JQG95_002626"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5889135.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(11667..12491)
                     /locus_tag="JQG95_002627"
     CDS             complement(11667..12491)
                     /locus_tag="JQG95_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY5889136.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            12811..14841
                     /gene="malS"
                     /locus_tag="JQG95_002628"
     CDS             12811..14841
                     /gene="malS"
                     /locus_tag="JQG95_002628"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY5889137.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            15019..16272
                     /gene="avtA"
                     /locus_tag="JQG95_002629"
     CDS             15019..16272
                     /gene="avtA"
                     /locus_tag="JQG95_002629"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY5889138.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(16423..16896)
                     /locus_tag="JQG95_002630"
     CDS             complement(16423..16896)
                     /locus_tag="JQG95_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5889139.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(16998..17846)
                     /gene="yiaJ"
                     /locus_tag="JQG95_002631"
     CDS             complement(16998..17846)
                     /gene="yiaJ"
                     /locus_tag="JQG95_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY5889140.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLSAIT"
     gene            18047..19045
                     /gene="yiaK"
                     /locus_tag="JQG95_002632"
     CDS             18047..19045
                     /gene="yiaK"
                     /locus_tag="JQG95_002632"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY5889141.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            19057..>19199
                     /locus_tag="JQG95_002633"
     CDS             19057..>19199
                     /locus_tag="JQG95_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY5889142.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         4917 a         4821 c         4664 g         4797 t
ORIGIN      
        1 gcagcggatt ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc tcaaagcagg
       61 tgaacaggaa gtccacatcc gcgtattcga agttgtaagt ggactgctcc acttcgttct
      121 gatggaacac gtcgccgtag gtggttttac ccagcgggcc gtcgctccag actaggtcgt
      181 aaacgctgtc tacgccctga atgtacatgg ccagacgttc cagaccgtag gtgatctcgc
      241 cggtaaccgg tttacactcc agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt
      301 ccatgccgtt tagccacact tcccagccca gtccccaggc ccccagagtc gggttttccc
      361 agttatcttc cacgaaacgg atgtcgtgaa tagtcgggtc catgcccagc tctttcagag
      421 aaccgaggta cagctcctga atattgtccg gcgatggctt aatgaccacc tggaactgat
      481 agtagtgctg taaacggttg gggttttcgc cgtagcgacc atcggtcggg cgacgagaag
      541 gctgaacata agcagccgcc atcggttctg gccccagcgc gcgcagacag gtcattgggt
      601 gagaggttcc cgcgccgact tccatgtcca atggttgaac aatggtgcag ccctggcgag
      661 cccagtaatc ctgtaaggtc aggatcaagc cctggaaggt cctggtatca aacttttgca
      721 tattatttcg tgctggatac gtgtggattt aaaggaaggg atcagtatac ccgctggatg
      781 gaagatatac agtacgaaac gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa
      841 tggccatctg cgtcaaaaga acagacaaac tgctctttac ggaaggtata accgcgcatt
      901 tcatagcttc cctggaactg ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta
      961 taacgttttg ccgcctgatc cttgcataac gcttccatat tcaacgaacg cagaggatca
     1021 acctttaccc gttgtgcttt ctgcacgggt gacggtgtag aacaacccac tagcaccata
     1081 agcgccattg ccggaaagaa agcgttcatc atcattaaat taccgtctgg tttgcaggtg
     1141 agtcttatta tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat
     1201 aggctgtagt gataaaactc agattattcc agtgcgccaa ccaaatgatt tatcggtcag
     1261 acttttttta accagataca gagacacaga tgcagcccaa aatttactgg attgataacc
     1321 tgcgagggat agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca
     1381 atgctcatag tgttagcccc gtcacatggg atatcgccaa tgttctgaat tctgcctctc
     1441 gtgtcagcgt gccgctattt ttcatgattt ccggctatct cttttttggc gaacgcagcg
     1501 cccagccgcg ccatttcttg cgtatcggct tatgtctgat tttttatagc gcaatcgcac
     1561 tgctctacat tgcgctgttt acctccatca atatggagtt agcgctgaaa aacctgctgc
     1621 aaaagccagt gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt
     1681 caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggtggtga
     1741 ttggcattat cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc
     1801 caattaactt atatatcaat ggcgatactt tttactacat tctgtatggc atgttgggcc
     1861 gcgctatagg gatgatggac acacagcata aagcactgtc gtgggtgagc gccgcgctgt
     1921 ttgcgacggg ggtttttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa
     1981 attttgccga tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc
     2041 tattgactct ggttaaaaac acgctggata cgcgtaccat tcgcggactt ggcttaatct
     2101 cccgccattc attgggtata tacggattcc acgccttgat tatccatgcg ctgcgcaccc
     2161 ggggaattga gcttaaaaat tggccaatac tggatattat ttggatcttt tgcgcgacgt
     2221 tggcagcgag tttgttactt tctatgctgg tacaacgaat cgacagaaac agattagtga
     2281 gttaagtaaa agcccgttca cattggactg accgggctta cgtgaattat tcgctgtatt
     2341 cttcctgggt aacctgtgtt tcttttggcg ggtttattcc ggcatcacga atattcttct
     2401 gcaccgctac tgcaccaaac aagcttaaga agaaagccag tccataaaaa cctttttcac
     2461 tgagtaataa tgtcgcattc cacagaccta ccatcagtag agcaacagag ataataaaga
     2521 cagtcaggca ggtcatataa taaatggaag tggtcggtat gccttcatac ttgtcccgaa
     2581 cggtcttttg ataagacgca gcagaaaaca gtcctaacac cagtacggca aaataatacc
     2641 ctttttcatt taactgcatc tctgcattcc atagccctaa caggtaggta acgataccac
     2701 caacgagagc tatccatgac acaatactaa aggccggtga ataggttgat attttgttgt
     2761 ccatcacagt attcctttca ttcctgaatg tgtaagagct ttatgttgct cataccgagc
     2821 gaaacaaacg catttgacca aacaagcaga caaaaaaggc gatggggtag atcaccattt
     2881 cataagcatt gataggtgca tttaaaacac cgataaacaa gagaccgata gtgattaacg
     2941 caactaactg gcagatatgg gtaaaaaaat tatccagttg cccgagtttt tctgcgcgaa
     3001 gatatgcata gttaccaaaa gttgctgtca ttaacacgcc aagaaaatag ccttttccac
     3061 ttagcaatgg gcatgagatc catagcccaa ccagataaac cagcgccccg acaacaaata
     3121 atagttttgc cacagttttt gaggttttca taacgatctc catatctacc agcgatacat
     3181 tacgagtaac caacgaaaga caaaactgaa aaatgccatt aacaaatgat tttcagaata
     3241 aattcatact aaatattaat taattagtga gatatataga tgtgaattat cccccacccg
     3301 gtcaggcagg ggataacgtt tacgccatta atggcagaag ttgctgatag aggcgacgga
     3361 acgtttcacg tcgtggctga taagcggcat aacgctgcgc atctggtaga tgcgactgtt
     3421 ctaacggtag ctgcggcaac aactcaatga gcgatttctc tggattcgcc gcaatctgcg
     3481 ccagccttgc tgcgcccagt gctggcccca catccccccc cgtacggtaa tcgagctgct
     3541 gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg ccaccaatca
     3601 acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca tctgccagcg
     3661 cataacccac gccttccagc actgctcgcg ccagttcatt ggggccatgt tgatgagtca
     3721 aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg ccggaaagat
     3781 aaggcagaaa ccaaactggc tcggcacttt catcagcctg ttgagctgca gcgattaaag
     3841 ctgggacatt gcacaggccg gttaatttcg cggcccaatc cagacacgat gctgcactca
     3901 gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg tgtacagcgc
     3961 tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac gtccccagcg
     4021 ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct gcattgtcgc
     4081 caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact tcaggtaaca
     4141 aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtcgc
     4201 aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac atggtgccag
     4261 ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag taatctttcg
     4321 gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga acccatagca
     4381 atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt gattgcggaa
     4441 ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag
     4501 ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg ccggcaatac
     4561 ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt gcgcgatcag
     4621 ttgcctgcca ccactgttcc gggtcttgtt ccgaccaaag tggatgcggg cgcgaaacgg
     4681 tcagcttttc cgtttgcgaa gcaaccacct caccctgctc gttgagcaaa ataactttta
     4741 cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc
     4801 ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag
     4861 ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca ccggagacaa
     4921 attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg
     4981 ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc
     5041 atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca tcgcgccgat
     5101 atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt
     5161 cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc
     5221 ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc
     5281 gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg
     5341 acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt ccagaccaaa
     5401 ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt tagtcggttc
     5461 ttgcggtttc ggttcgataa gcaacgtgcc ctagaagccg attttatgtt tatgctcaac
     5521 caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg tatttaacag
     5581 cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca atttatgggt
     5641 tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag gatctgggtt
     5701 cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcggttc cccacagcag
     5761 cttcacgccg ctctcttctt gcttggctgc caggacatca accatttgcg caaaattatt
     5821 gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa
     5881 tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc
     5941 cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa acatgtccgc
     6001 cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg
     6061 cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt
     6121 tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat
     6181 aatcaggtaa tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcaattg
     6241 ctcaattatg ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa
     6301 aataaaatgg gatgatgaaa ctgggtaatt cctcgaagat aaaaatgcaa taagtacaat
     6361 tgcgcaacaa aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga
     6421 aagataaaaa tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc
     6481 gcggtgattg ttacttatta aagctgtcct ctaactacag aaggccctac accatgaaaa
     6541 taaagaacat tctactcacc ctttgcacct cacttctgct taccaacgtt gcggcacacg
     6601 ccaaagaagt caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag
     6661 atcgagatat ttttgtgaaa aaggcagaat ctctcggtgc gaaagtattt gtacagtctg
     6721 caaatggcaa tgaagaaaca caaatgtcgc agattgaaaa catgattaac cggggtgtcg
     6781 atgttcttgt cattattccg tataacggtc aggtattaag taacgttgta aaaaaagcca
     6841 aacaagaagg tattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt
     6901 tttatatttc tttcgataac gaaaaagtcg gtgaactgca ggcaaaagcc ttggtcgata
     6961 ttgttccgca aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc
     7021 tgttccgcgc cggacaaatg aaagtgttaa aaccttacgt tgattctgga aaaattaaag
     7081 tcgttggtga ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa
     7141 acgcgctaac cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg
     7201 caggtggggc aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatttccg
     7261 gccaggatgc ggatctcgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg
     7321 tgtataaacc tattacattg ctggcaaata ctgccgcaga aattgccgtt gaattgggca
     7381 atggtcagga gccaaaagca gatacctcac tgaataatgg cctgaaagat gtcccctccc
     7441 gcctcctgac accgatcgat gtgaataaaa acaacatcaa agatacggta attaaagacg
     7501 gattccacaa agagagcgag ctgtaagcgt tacgccccgg cgcggagcgg gggcgtgatt
     7561 tctctccatg ccgcgtgaat gaattggctt aggtggagtc gttatgtctt atctacttga
     7621 aatgaagaac attaccaaaa ccttcggcag tgtgaaggcg attgataacg tcagcttgcg
     7681 gttgaatgct ggcgaaatcg tctcactctg tggggaaaat gggtctggta aatcaacgct
     7741 gatgaaagtg ctgtgtggta tttatcccca tggctcctac gaaggcgaaa ttatttttgc
     7801 gggagaagag attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccatcat
     7861 tcaccaggaa ttggccctgg tgaaagaatt gaccgtgctg gaaaatatct tcctgggtaa
     7921 cgaaataacc cacaatggca ttatggatta tgacctgatg acgctacgct atcagaagct
     7981 gctcgcacag gtcagtttat ccatttcacc tgatacccgc gttggcgatt tagggcttgg
     8041 gcaacaacaa ctggttgaaa ttgccaaggc acttaataaa caggtgcgct tgttaattct
     8101 cgatgaaccg acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg
     8161 cgatctacaa cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa
     8221 agcgatttcc gatacgattt gcgttattcg cgacggacag cacattggta cgcgtgatgc
     8281 tgccggaatg agtgaagacg atattatcac catgatggtc gggcgagagt taaccgcgat
     8341 ttaccctaat gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc
     8401 atggcatccg gtcaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg
     8461 tggcgaaata ctgggtattg ccggactcgt tggtgccgga cgtaccgaga ccattcagtg
     8521 cctgtttggc gtgtggcccg gacaatggga aggaaaaatt tatattgatg gcaaacaggt
     8581 agatattcgt aactgtcagc aagccatcgc ccaggggatt gcgatggtac ccgaagacag
     8641 aaagcgcgac ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact
     8701 caataaattt accggtggca ttagccagct tgatgacgcg gcagagcaaa aatgtattct
     8761 ggaatcaatt cagcaactca aagttaaaac gtcgtccccc gaccttgcta ttggacgttt
     8821 gagcggcggc aatcagcaaa aagcgatcct cgcccgctgt ctgttactta acccgcgcat
     8881 cctcattctt gatgaaccta ccaggggtat tgatattggc gcgaaatatg agatctacaa
     8941 attaattaac caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc
     9001 tgaagtgctc ggccttagcg atcgtgtact ggtgatgcat gaagggaaac taaaagccaa
     9061 cctgataaat cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca
     9121 tcatgtcgaa aagcaatccg tctgaagtga aattggccgt accgacatcc ggtggctttt
     9181 ctgggctgaa atcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca
     9241 tgctgttctt tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc
     9301 tgttacgcca gaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt
     9361 ctgctgaaat cgacctttcc gtcggctcaa tgatggggct attaggtggc gtcgcggcga
     9421 tttgtgacgt ctggttaggc tggcctttgc cacttaccat cattgtgacg ttggttctgg
     9481 gactgcttct cggtgcctgg aacggatggt gggtcgcgta ccgcaaagtc ccttcattta
     9541 ttgtcaccct cgcgggcatg ttggcatttc gcggcatact cattggcatc accaacggca
     9601 cgactgtatc ccccaccagc gccgcgatgt cacaaattgg gcaaagctat ctccccgcca
     9661 gtaccggctt catcattggc gcgcttggct taatggcttt tgttggttgg caatggcgcg
     9721 gaagaatgcg ccgtcaggct ttgggtttac agtctccggc ctctaccgca gtagtcggtc
     9781 gccaagcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg
     9841 gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa
     9901 cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacgtc
     9961 tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg
    10021 tagccatcgc cggattaatc cttagttctc gacttggcgc tggttcacct tctgcgggaa
    10081 atatcgccga actggacgca attgcagcat gcgtgattgg cggcaccagc ctggctggcg
    10141 gtgtgggaag cgttgccgga gcagtaatgg gggcatttat catggcttca ctggataacg
    10201 gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt
    10261 tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt
    10321 ctcaaagccg gttacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac
    10381 catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg
    10441 gcaggtagta gaaggcgtag gggaatattt acaggcatca caatcagaat gggatatttt
    10501 cattgaagaa gatttccgcg cccgcattga taaaatcaag gactgtttag gagatgtcgt
    10561 cattgccgac ttcgacgaca aacagatcga gcaagcgctg gctgatgtcg acgtccccat
    10621 tgttggggtt ggcggctcgt atcaccttgc agaaagttac ccacccgttc attacattgc
    10681 caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaagagaa aggcgttaac
    10741 cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac tgaacgcgaa
    10801 tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta
    10861 gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg actacaaacg
    10921 ctgccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca tattctgcaa
    10981 gtatgtgaac atatacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac
    11041 gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca gggcgcgcgg
    11101 caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg
    11161 ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc
    11221 tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa
    11281 gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag
    11341 cgttttaaag aagaggtggg tgaaaccatc catgcaatga ttcatgccga gaagctggag
    11401 aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc
    11461 ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga cacaacgcca
    11521 aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga attgtcggcg
    11581 tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc cgaccgacaa
    11641 atcagataag gctcgaggaa atgaaattac atatgcgccg cgattaaccg ttggttatcc
    11701 tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta gcccttcagc
    11761 ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg tgcacgcgat
    11821 ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc gctcaccgat
    11881 tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt ggtacaacgt
    11941 cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt cgacgttccc
    12001 caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat gtctacgcgt
    12061 tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac cttgtagcgt
    12121 ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg atactgtttt
    12181 gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat gtaaggcatt
    12241 acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg caaatcagga
    12301 agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac ctgtttatta
    12361 ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt cgctaacacc
    12421 tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg tcgtatggga
    12481 gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat atttttcaca
    12541 aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac cccaaggaat
    12601 agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa atcgcagaaa
    12661 atgtgggggt tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac ccttgcttct
    12721 catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg gcaaactgtc
    12781 tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt tctgacactc
    12841 cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc ctttagcgaa
    12901 cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg tccactcacg
    12961 ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa tcagatgctt
    13021 tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga cggcaaatat
    13081 acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat ccagaacgcc
    13141 gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt accgctcacg
    13201 ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta cagccaacaa
    13261 attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag caacggttta
    13321 ctcctgctgg aacgggcaga aactgacaca tccgcccctt tcgactggca taacgccacg
    13381 gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga ccagagttac
    13441 ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga tttacgcggc
    13501 ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg gataagcgcc
    13561 ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt cccgcattat
    13621 gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat gggcaacgaa
    13681 gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat tctctttgat
    13741 gtcgtgatga accacaccag ctatgccacg ctggcggata tgcaggagta tcagtttggc
    13801 gcgttatatc tttctggtga cgaagtgaaa aaaacgctgg gtgaacgctg gagcgactgg
    13861 aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt cagcgacaaa
    13921 acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg cgattacgac
    13981 aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat caaaaccgaa
    14041 tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac tcacgctaaa
    14101 gtcatcgaag gctttacgcc tcgcgattac ttaacccact ggttaagtca gtgggtccgc
    14161 gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt gcccgcctgg
    14221 cagcaactga aaaccgaagc cagctccgcg cttcgcgaat ggaaaaaaac taaccccgac
    14281 aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca cggcgtgatg
    14341 caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta tcaggagcag
    14401 gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca aatggcggag
    14461 aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg tctgttccgt
    14521 gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt acaaatcttt
    14581 tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct gcaaggtaca
    14641 cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt cgcgcactgg
    14701 cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca
    14761 ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa agtgctggtc
    14821 atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct cgggaagccg
    14881 ccaaccagga taaaatgtca gccctaatca gcgttgcggg ataaagcacc gctcactctt
    14941 caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct tgctacagag
    15001 ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac ccgccactcc
    15061 ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg cgcgattatg
    15121 ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca gacgctactg
    15181 accgacatgc tggaaagtgg caaagcgact gatgcactgt gtaactacga cggtccacag
    15241 gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt gggttgggat
    15301 atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt ctacttattt
    15361 aacctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt cccgctggca
    15421 ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt ctctgcgcgt
    15481 ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt tgagcatctg
    15541 catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc aacaggcaat
    15601 gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca cggcattccg
    15661 ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag tgaagggcgc
    15721 ccgctatgga atccgaatat cgtactgtgc atgagtcttt ccaagctggg gctgcctggc
    15781 tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ctgccatcac caatatgaac
    15841 ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga aatgattaag
    15901 cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta ccagcgtgtt
    15961 caggaaacta tcgccatcat tcgccgctat ttaccggaaa atcgctgcct gattcataaa
    16021 ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac gaccgagcag
    16081 ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa cttcttcccg
    16141 ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta cgtaccagag
    16201 ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag agcctgggct
    16261 gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga tttctgcaat
    16321 atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat ctggcataaa
    16381 caaagcgcac tattccggcg ttgagaaacg ccggaaaacg tttcaaacag gctgctgttg
    16441 tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg tcgggcaggc
    16501 ttcaacacag gccggccccg tctcccgatg ccagcacaga tcgcacttaa tcgccctcgc
    16561 ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca tacagctttt
    16621 acagccaatg caacgtgttt gttcaacgaa aatatgccca tgttcgcggc ttatcgcgtc
    16681 aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta ctggcgtggt
    16741 ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg acaacgcagc
    16801 gcaatcctga ttctcctgat gcgacactgc gcaagccact tcacaggtac ggcaaccgat
    16861 acatttcgtc gcatccgcaa taataaaccg gttcatgatc attcctttgc actaaaacag
    16921 cagagtgcca gagagcgcgt ccctactgct ttgatccaac gaaggaaatg ttaaagtttt
    16981 ggcacttgtc caaagcgtta tgtgatcgcg ctcaggtcat ccctgacagt aaatcccagt
    17041 tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt ctctcccacc
    17101 tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt acggcacccg cccatgaata
    17161 tcaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct gtccatcgcc
    17221 gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc ggtaatggta
    17281 ttgcgggtta acggctggat ctcatgctga tggttttccc agtatgactt cacgtagtcc
    17341 gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg catatgctgg
    17401 ccaatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata aatcaaaata
    17461 gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat gttcagtgcc
    17521 tcaagatgcg gagcggcgat atgaatgata ttcagcgaag acagcgcctt ctgcccgacg
    17581 gcaataaatt tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt cacatagccg
    17641 caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc agccagctcc
    17701 gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat cagcatcaac
    17761 ccgcgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat ctcgttctct
    17821 ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc aaagtgtgcc
    17881 gtagttcacg atctcgacag ataatttata accacttgat ttttatgtct tttgaaattc
    17941 atcaatcaga ttgctttgtt aaaaagtgat cgatatattt gaaatctagt tccgcatatt
    18001 gaaattttaa gccaaaaaag cgatcaaaaa aacaaggaag cctaggatga aagtgacatt
    18061 tgagcagtta aaagcagcct ttaatcgggt cttaatttca cgcggcgttg acagcgaaac
    18121 ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt attctcacgg
    18181 cgttaatcgt ttccctcgtt tcattcaaca actggaaaac ggcgatatca ttcctgatgc
    18241 ccaacccaaa cgtataacca gcctcggcgc aattgaacag tgggatgccc agcgttcgat
    18301 cggcaacctg acggcgaaaa agatgatgga tcgcgccatt gaactggctg ccgatcacgg
    18361 tattggtctg gtggcacttc gtaatgccaa ccactggatg cgcggcggca gctacggctg
    18421 gcaggcggcg gaaaaaggct atattggcat ttgctggacc aactccatcg ccgtaatgcc
    18481 gccgtggggc gcaaaagagt gtcgcatcgg cactaacccg ctgatcgtcg ccattccttc
    18541 cacgccgatc accatggtcg atatgtcgat gtcgatgttc tcttacggca tgttagaagt
    18601 taaccgcctg gcaggccgtc agctcccggt cgatggtggc tttgatgatg agggcaattt
    18661 gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg gctactggaa
    18721 aggttctggc atgtcgattg tgctggatat gatcgctact ctcctttccg acggcgcatc
    18781 ggttgccgaa gtcacccagg acaacagcga cgaatacggc atttcacaaa tttttattgc
    18841 cattgaagtg gacaagctta tcgacggtcc cacccgcgat gccaagctgc aacgtatcat
    18901 ggattacgtt actaccgctg aacgcgctga cgaaaaccag gccatccgct tacccggcca
    18961 cgaatttact accctgctgg ccgaaaaccg ccgtaacggc atcactgttg atgacagcgt
    19021 gtgggccaaa atccaggcgt tatgaggaga taagtcatga tttttggaca tatcgcgcag
    19081 cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc tcgattttct gcgcgctacc
    19141 aacttcaacg tcctggagcc gggcgttgtc gaaatcgacg gcaagaatat ttatgcgca
//
LOCUS       DADLCQ010000082        19180 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000082 DADLCQ010000000
VERSION     DADLCQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.082"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..422)
                     /locus_tag="JQG95_002634"
     CDS             complement(<1..422)
                     /locus_tag="JQG95_002634"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5889143.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     YLHKVTTTVSKNHVMIVIEDLKVSNMSKSAAGTVSQPGR"
     gene            complement(687..860)
                     /locus_tag="JQG95_002635"
     CDS             complement(687..860)
                     /locus_tag="JQG95_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5889144.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            928..1543
                     /gene="tnpA"
                     /locus_tag="JQG95_002636"
                     /pseudo
     CDS             928..1543
                     /gene="tnpA"
                     /locus_tag="JQG95_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1871..2953
                     /locus_tag="JQG95_002637"
     CDS             1871..2953
                     /locus_tag="JQG95_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY5889145.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3442..4240)
                     /locus_tag="JQG95_002638"
                     /pseudo
     CDS             complement(3442..4240)
                     /locus_tag="JQG95_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4250..4801)
                     /gene="ybcL"
                     /locus_tag="JQG95_002639"
     CDS             complement(4250..4801)
                     /gene="ybcL"
                     /locus_tag="JQG95_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY5889146.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4970..5302)
                     /gene="emrE"
                     /locus_tag="JQG95_002640"
     CDS             complement(4970..5302)
                     /gene="emrE"
                     /locus_tag="JQG95_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY5889147.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5647..5961)
                     /gene="fliE"
                     /locus_tag="JQG95_002641"
     CDS             complement(5647..5961)
                     /gene="fliE"
                     /locus_tag="JQG95_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY5889148.1"
                     /translation="MSAIQGIEGVISQLQATAMSACAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            6175..7840
                     /gene="fliF"
                     /locus_tag="JQG95_002642"
                     /pseudo
     CDS             6175..7840
                     /gene="fliF"
                     /locus_tag="JQG95_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            7833..8828
                     /gene="fliG"
                     /locus_tag="JQG95_002643"
     CDS             7833..8828
                     /gene="fliG"
                     /locus_tag="JQG95_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY5889149.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8821..9507
                     /gene="fliH"
                     /locus_tag="JQG95_002644"
     CDS             8821..9507
                     /gene="fliH"
                     /locus_tag="JQG95_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY5889150.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9507..10880
                     /gene="fliI"
                     /locus_tag="JQG95_002645"
     CDS             9507..10880
                     /gene="fliI"
                     /locus_tag="JQG95_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY5889151.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10902..11243
                     /gene="fliJ"
                     /locus_tag="JQG95_002646"
                     /pseudo
     CDS             10902..11243
                     /gene="fliJ"
                     /locus_tag="JQG95_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11240..12367
                     /gene="fliK"
                     /locus_tag="JQG95_002647"
     CDS             11240..12367
                     /gene="fliK"
                     /locus_tag="JQG95_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY5889152.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12472..12936
                     /gene="fliL"
                     /locus_tag="JQG95_002648"
     CDS             12472..12936
                     /gene="fliL"
                     /locus_tag="JQG95_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY5889153.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12941..13945
                     /gene="fliM"
                     /locus_tag="JQG95_002649"
     CDS             12941..13945
                     /gene="fliM"
                     /locus_tag="JQG95_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY5889154.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13942..14355
                     /gene="fliN"
                     /locus_tag="JQG95_002650"
     CDS             13942..14355
                     /gene="fliN"
                     /locus_tag="JQG95_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY5889155.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14358..14723
                     /gene="fliO"
                     /locus_tag="JQG95_002651"
     CDS             14358..14723
                     /gene="fliO"
                     /locus_tag="JQG95_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY5889156.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14723..15460
                     /gene="fliP"
                     /locus_tag="JQG95_002652"
     CDS             14723..15460
                     /gene="fliP"
                     /locus_tag="JQG95_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY5889157.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVINKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15470..15739
                     /gene="fliQ"
                     /locus_tag="JQG95_002653"
     CDS             15470..15739
                     /gene="fliQ"
                     /locus_tag="JQG95_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY5889158.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15748..16533
                     /gene="fliR"
                     /locus_tag="JQG95_002654"
     CDS             15748..16533
                     /gene="fliR"
                     /locus_tag="JQG95_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY5889159.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16818..17441
                     /gene="rcsA"
                     /locus_tag="JQG95_002655"
     CDS             16818..17441
                     /gene="rcsA"
                     /locus_tag="JQG95_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY5889160.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGNILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17485..17727)
                     /gene="dsrB"
                     /locus_tag="JQG95_002656"
     CDS             complement(17485..17727)
                     /gene="dsrB"
                     /locus_tag="JQG95_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY5889161.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            17836..18063
                     /gene="yodD"
                     /locus_tag="JQG95_002657"
     CDS             17836..18063
                     /gene="yodD"
                     /locus_tag="JQG95_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY5889162.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18361..19176
                     /locus_tag="JQG95_002658"
                     /pseudo
     CDS             18361..19176
                     /locus_tag="JQG95_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
BASE COUNT         4937 a         4741 c         4747 g         4755 t
ORIGIN      
        1 cgccccggct ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc
       61 tcaatgacta tcattacgtg gtttttgctg acggtcgttg tgactttgtg aaggtagtcc
      121 ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg cttctgccag
      181 ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag cttcttctgg
      241 tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcga ggcaagttta
      301 gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt tgatacttca
      361 ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt gacatttttc
      421 acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt tggcagaaaa
      481 atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc atcattctgt
      541 caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt gcaggcctgc
      601 tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat ccattgcgtt
      661 tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg gatgtctttg
      721 ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac gaaacgacat
      781 gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa ccggaatttg
      841 aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa aactgatatc
      901 cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt taccaagtac
      961 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
     1021 tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatctg
     1081 ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca gcctcaaagg
     1141 cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt attaccgcaa
     1201 agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc caatatccgt
     1261 catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac gagccttata
     1321 tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga ttatctacac
     1381 ctgcatggac atcattgtac tatgccccgt acatgcccca tcacatcctc aacatcaaag
     1441 tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa
     1501 aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc tcaattacat
     1561 taataaatct aaataaatat catgcaaata atttccatgg ttatttttag gctatttaaa
     1621 ttaaaaaaat aaattatgag tcaatcataa ataacaacct ttaatataat ttgactacca
     1681 aatttatatt cgttcacaaa atagtcatgc attgtgtgca atagaaacag ttcagataaa
     1741 aatagggatc tacttcacaa tttaaatgag aaactaaaac ttacatcttg aaataatctc
     1801 attgattgga tgaatatttg tagtgcagtg acatcatttt taaataatag ttcaaataaa
     1861 agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat cagtactgat
     1921 ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc tggatctgta
     1981 cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg gcgacacaac
     2041 ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg
     2101 tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt ccaaagataa
     2161 aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg attatggccg
     2221 taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag aattcggtgg
     2281 cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg ttgcaaccta
     2341 tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc agtatcaggg
     2401 taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt tctccactac
     2461 ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc gcactaatga
     2521 tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg aagtatgggc
     2581 agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt ctgaaaccca
     2641 gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact tcgaagtagt
     2701 tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg
     2761 aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa tatattgatg taggtgcaac
     2821 ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc tgattgataa
     2881 gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg taggattggt
     2941 ttaccagttc taatctgatt actcaaagat atgttgtggg aggctttgcc tccccaacat
     3001 ataagtggcg ctctcaagcc actttcctta gaagcactat cttgcttctg actatataaa
     3061 ttacccttta ttttctggga cgtttcaacg ccccattttt aataatactc agtgaacaat
     3121 taacgtatca attagattta ataacaacga tatccgtacc taacctgata tctaatgtca
     3181 taagcatccc tttaattatc ccctcaacct cttgtaaatt tcccactctt gagctccaca
     3241 tagcccaaac gccggatgca aaaaaaatca cacgtgcatc tctgaaattg tgatggagga
     3301 agtgagtatc aattatatgt gcttataaac gttcccgatt ggcacagaag tcaccggatt
     3361 gttcaggctc caataggttg agtatgtcgt atttattttc caaaataaaa atcgatgctg
     3421 ttgctcctta ataaaatact tttactctct ggacgctttt cttagctctc ttggtgttat
     3481 attaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac cattttccct
     3541 ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca ggacaacgca ttatttaacc
     3601 tcacgtcgtc caatatactt tgaaaactta ttccctcgct tgccagacgg cgatgcaatg
     3661 tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt gttctaggtt
     3721 cggatagcat taggttataa caacgactta ttaatgattg tttactatct gataaaagta
     3781 aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa
     3841 tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa tatattaact
     3901 cggggttctg aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa
     3961 atttttgaaa cttttcaatt agatcaatgg gataataaag catttctgca agataaagac
     4021 ctgcttctgg ataattcaca atataaaatt catatccaca gggaaataat attatctgat
     4081 tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc tgacggatac
     4141 gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta tgcatccgta
     4201 tttcttcaat ctttaggtta gacttatctc ttgccagcat actcttaccc tattttatct
     4261 cataaactgg tgttatctca gcggttgcaa ttttattggc attaagcata taaccaacta
     4321 acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc catactttaa
     4381 actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat cctgcataac
     4441 caaaatcatt tcggccttga acagcaccag tcggaagttt tgtaccatca cgtcttcctg
     4501 catcagtggg caaatatgtt acagttgctg gaatattagc aacagtccag tgccaccatc
     4561 cactgcctgt aggagcatct ggatcataca cagttacggc aaagcttttg gtgccttcag
     4621 gagcaccaga ccaggttaat gagggcgatg tattaccacc ttcacaccca aatccagaaa
     4681 atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg acctgaaatg
     4741 ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata agttttttca
     4801 taaaaacctc attgttgtga cctatcgtta ttttatttga tgtgccttga tctcattatg
     4861 aataaaggcg caatgatcgc gcaaatgcaa tcataattat aaaggtgccc cgaacattag
     4921 ccataatatg cacaaatcag attcctgtta tctcaaggag aattattttt taatgcggtg
     4981 cacttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc atgcctatga
     5041 tagctggcag gtccagtcgt tgaccaaaaa atccccagga aagcaaacta atcagaacga
     5101 taccgactcc tgaccagata gcataagcaa tccctgtagg tatataagcc agcgtctgag
     5161 ctaataacca gaatgatgca caatagcaaa taattgtgcc aacagagggc cataaccgtg
     5221 taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt attgcgccac
     5281 caagataaat ataagggttc atagcatatt ctttcctgtt catactggag agaattgtac
     5341 tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccaa ggggctctgg
     5401 cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg ctaacgaatg
     5461 agttcactct tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg
     5521 gatggcagaa aacaaccagg atcaggtatc cattttaaac cagaaattga gtgagtttgc
     5581 cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt caacaggtta
     5641 cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct tattacgcac
     5701 ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca catcgtttag
     5761 cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcga ctgtttgtgt
     5821 atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg
     5881 cagtgattcc tgcgcacacg cactcatcgc cgtagcctgt aactggctga taaccccttc
     5941 aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctggtaaca aagcctacca
     6001 gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa actggtttat tggcggatag
     6061 aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtttgctga cccgagagtg
     6121 agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaggtg cgcgatgaat
     6181 gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa
     6241 attccattga ttgttaccgg ttccgcggca gtggcggtca tggtcgcact gatcctgtgg
     6301 gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga tggtggcgca
     6361 attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc cagcggcgct
     6421 attgaggttc cggcagataa agttcacgaa ctgcgcctgc gcctggcgca acaaggtttg
     6481 cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag
     6541 ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa
     6601 actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc
     6661 gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca
     6721 ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg
     6781 ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc
     6841 agcgggcgcg atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt
     6901 cagcagcgta ttgaagcgat cctgtcacct attgttggta acggtaatat tcacgcctag
     6961 gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac
     7021 ggtgatgaat ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt
     7081 tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac
     7141 gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc agcagcaggc gagcaccacc
     7201 agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc
     7261 accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg
     7321 gtgaattaca aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag
     7381 caaattgaag ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc
     7441 aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagcact gccgttttgg
     7501 caacagcaag tgtttatcga tcagttgctg gctgccggtc gctggttgct ggttgctggt
     7561 actgctggtg gcgtggttgc tgtggcggaa agcggtacgc ccgcagctaa cacgtcgcgc
     7621 cgaggcggtg aagactgtgc agcaacaggc gcaggcccac gaggaagtgg aagatgcggt
     7681 ggaagtccgt ctgagcaaag acgaacaact ccaacaacgg cgcgctaacc aacgactggg
     7741 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
     7801 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
     7861 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
     7921 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
     7981 agcagctaac cgatgtgctg gcggagtttg agcaagaagc tgaacagttt gctgcactga
     8041 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaagaacgtg
     8101 ccgccagcct gctggaagat attctcgaaa cccgcgatac cgccagcggt attgaaacgc
     8161 tcaactttat ggagccgcag agcgccgccg atctgattcg cgatgagcat ccgcaaatta
     8221 tcgccaccat tctggtgcat ctgaagcgcg cccaggccgc cgatattctg gcgttgttcg
     8281 atgaacgtct gcgccacgac gtgatgttgc gtatcgccac ctttggcggc gtgcagccag
     8341 ccgcgctggc cgaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     8401 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgatg aaaacgcagc
     8461 aggaagaagc cgttattacc gccgtgcgtg aattcgacgg cgagctggcg cagaaaatca
     8521 tcgacgagat gttcctgttc gagaatctgg tggatgtcga tgatcgcagc attcagcgtc
     8581 tgttgcagga agtggattcc gaatcgctgc tgatcgcgct gaaaggggcc gagcagccac
     8641 tgcgcgagaa attcctgcgc aatatgtcgc agcgtgccgc cgatattctg cgcgacgatc
     8701 tcgccaaccg tggtccggtg cgtctgtcgc aggtcgaaaa cgaacagaaa gcgattctgc
     8761 tgattgtgcg ccgtcttgcc gaaactggcg agatggtgat tggcagcggc gaggatacct
     8821 atgtctgata atctgccgtg gaaaacctgg acgccggacg atctcgcgcc accaccggca
     8881 gagtttgtgc caatggtcga gtcggaagaa accattattg aagaggccga acccagcctt
     8941 gagcagcaac tggcgcaact gcaaatgcag gcccacgagc agggttatca ggcggggatt
     9001 gccgaaggtc gccagcaagg tcacgaacag ggctatcagg aaggactggc ccaggggctg
     9061 gaacaaggtc tggcagaggc gaaggctcaa caagcgccaa ttcatgcccg gatgcagcaa
     9121 ctggtcagcg aatttcaaac tacccttgat gcgcttgata gtgtgatagc ttcgcgcctg
     9181 atgcagatgg cgctggaggc agcacgtcag gtgatcggtc agacaccaac ggtggataac
     9241 tcggcactga tcaaacagat ccaacagttg ttgcagcaag aaccgttatt cagcgggaaa
     9301 ccacagctgc gcgtacaccc ggatgatctg caacgtgtgg atgatatgct cggcgcaact
     9361 ttaagtttgc atggctggcg cttgcggggc gatcccaccc tccaccctgg cggatgtaaa
     9421 gtctccgccg atgaaggcga tctcgacgcc agtgtcgcca ctcgctggca agaactctgc
     9481 cgtctggcag caccaggagt ggtgtaatga ccacgcgcct gactcgctgg ctaaccacgc
     9541 tggataactt tgaagccaaa atggcgcagt tgcctgcggt acgtcgctac gggcgattaa
     9601 cccgcgctac cgggctggtg ctggaagcca ccggattaca attgccgctc ggcgcaacct
     9661 gtgtcattga gcgccagaac ggcagcgaaa cgcacgaagt agaaagcgaa gtcgttggct
     9721 ttaacggtca acggctgttt ttaatgccgc tggaggaagt ggaaggtgtc ctgcccggcg
     9781 cgcgtgtata tgccaaaaac atttcggcag aagggctgca aagcggcaag cagttgccgc
     9841 tcggtccggc gttattaggt cgtgttctgg acggtagcgg taaaccgctc gatggcctgc
     9901 ccttccccga tacgacggaa accggtgcgc tgattacccc gccatttaac ccgttgcaac
     9961 gtacaccgat tgaacatgtg ctggacaccg gcgtgcgccc aatcaatgcc ctgcttaccg
    10021 tcggacgcgg gcagcgtatg gggctgtttg ccgggtcggg cgttggtaaa agtgtgctgc
    10081 tggggatgat ggcacgctac acccgcgccg atgtcattgt cgtgggtttg attggtgaac
    10141 gtgggcgcga agtaaaagat tttattgaga atatcctcgg tgccgaagga cgtgcacgct
    10201 cagtggtgat tgccgctccg gcggatgttt ctccgctcct gcgaatgcaa ggtgccgcct
    10261 atgccacgcg cattgccgaa gattttcgcg atcgtggtca gcatgtgttg ctgattatgg
    10321 actccctcac ccgctacgcg atggcccagc gtgaaattgc gctggcgatt ggcgaaccac
    10381 ctgccactaa aggttatcca ccgtcggtgt ttgccaaatt accggcactg gtcgagcgtg
    10441 ccggtaatgg cattagcggc ggcggctcga ttaccgcgtt ttataccgtg ctcaccgaag
    10501 gcgatgacca gcaggacccc attgctgact ccgcgcgggc aatcctcgac ggtcacattg
    10561 tgctgtctcg ccgactggcg gaagccgggc actatccggc tatcgatatt gaagcgtcga
    10621 tcagtcgcgc aatgacggcg ttgatcagtg agcaacatta cgcgcgagtg cgcaccttca
    10681 aacagctgtt gtcgagtttt cagcgtaacc gcgatctggt tagcgtcggc gcgtatgcca
    10741 aaggcagcga tccgatgctc gataaagcca tcgccctgtg gccgcagctg gagggctatt
    10801 tgcaacaagg catttttgaa cgcgcggact gggaagcgtc tctccagggg ctggagcgta
    10861 ttttcccgac agtgtcataa cccaggagat aacggcagat ggcggaagaa cagctcaaaa
    10921 tgttgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
    10981 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
    11041 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
    11101 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
    11161 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
    11221 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
    11281 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctca cgttgttgag
    11341 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtagcaac
    11401 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
    11461 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
    11521 atcaacaccg ttaaccaccg ctcagacgat gacgttggct gcggtggctg acaaaaatac
    11581 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
    11641 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
    11701 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
    11761 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
    11821 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
    11881 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc
    11941 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
    12001 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
    12061 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
    12121 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
    12181 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
    12241 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggaag acgacgatac
    12301 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
    12361 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
    12421 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
    12481 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
    12541 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
    12601 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
    12661 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
    12721 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
    12781 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
    12841 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
    12901 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
    12961 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
    13021 cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
    13081 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
    13141 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
    13201 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
    13261 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
    13321 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
    13381 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
    13441 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
    13501 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
    13561 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
    13621 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
    13681 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
    13741 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
    13801 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
    13861 tactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
    13921 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
    13981 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
    14041 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
    14101 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
    14161 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
    14221 ggcgaaccgc tggatattct gatcaacggt tatttaatcg cccagggcga agtggtggtt
    14281 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
    14341 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
    14401 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
    14461 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
    14521 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
    14581 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
    14641 cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
    14701 ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg
    14761 gctgattacg cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg
    14821 cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt
    14881 cattccagcg attttactga tgatgaccag tttcacccgc atcattattg tttttggttt
    14941 attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc
    15001 actgtttttg acctttttta ttatgtcgcc agtgatcaac aaaatttatg tcgatgcgta
    15061 ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc
    15121 gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact
    15181 ggcgaatacc ggcccgttgc aggggcctga agctgtgccg atgcgcattt tgctcccggc
    15241 ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt
    15301 tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc
    15361 ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca
    15421 attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga
    15481 atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct
    15541 gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat
    15601 taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat
    15661 tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct
    15721 gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc
    15781 tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca
    15841 attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg
    15901 ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg
    15961 tggctggccg tacagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc
    16021 tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg
    16081 acatttgtcg atcctggcag ccatctcaat atgcccgttt tagcgcgcat catggatatg
    16141 ctggcattac tgctgttcct gacatttaac ggtcatttat ggttgatttc actgctggtc
    16201 gatacctttc acaccctgcc gattggtagc gaaccgttga acagcaatgc gtttctggca
    16261 cccaccaaag cagggagttt gattttcctt aacgggctga tgctggcgtt accgctcatt
    16321 actctgttgc tgaccctgaa tctggcatta ggtttactga atcgtatggc cccgcaatta
    16381 tccatttttg ttattggatt tccattaact ctgactgtcg gcatctcttt aatggcggca
    16441 ttaatgccgt taattgcgcc tttttgcgaa catttattca gtgaaatttt taatttgctg
    16501 gctgatatta ttagtgaatt accattaata taattccgta acgtttatca tgctacccta
    16561 aggattatcc gaaaaataat atctgcgaac atcttccagg atactcctgt agcgaaatat
    16621 ttgttttaag ctcactcaca tatcgcaaca tttactttaa gacaattcca ggcaaattat
    16681 acaacacttt acgggatagt aagtccgcct gaaaaattgc gagagtggcg cattaggtga
    16741 cccatgttgt tccgtttagt catgatgaaa tattcaggta aggggaatta tcgttacgca
    16801 ttgagtgagg gtatgccatg tcaacgatta ttatggattt atgtagttac acccgactag
    16861 gtttaaccgg gtatctgttg agtagagggg ttaaaaaaag agaaatcaac gacattgaaa
    16921 ccgttgatga ccttgccata gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg
    16981 aggactgttt catccacgat gcttctaaca gtcagcgcat caagcacatc attaatcaac
    17041 atcccaatac gttatttatc gtttttatgg caattgccaa tgttcatttt gatgaatatc
    17101 tattggtcag aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg
    17161 atatccttgg caatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga
    17221 cgttatcatt gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa
    17281 ccattcaaat ctccgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta
    17341 atattaaacg taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga
    17401 cggataatgt gactaatggt atttttgtca acatgcgcta acacattctg actggtgatt
    17461 tcccaccagt caggctgaat aagattactc tgctttctca acaaagatac cgtcctgatg
    17521 ccctgcttca ttaaagaacc agatgccgag cgggtagtct tccagcgcaa ccaggtacat
    17581 tgtgccttca ctaaactcct caactgccag taccactcca ggacgacgcg gaccgccatc
    17641 cgttttgact gttacccgat cattcacctt catcgttttc ctcctgtggc tttgtgccag
    17701 tgtagaacaa tttcgttttt ctggcagcgc caagcgcgcg cgagtgctga ttttctcgac
    17761 ggtctatact taagagatgc taggcggact taacgactgg cggcaacaac agagtaacgg
    17821 ttgcgaggaa agatgatgaa aaccgcaaaa gtgtacagcg ataccgcaaa acgtgaggtc
    17881 agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa ttagcgaaag cgaagttcat
    17941 cgcagccaga acgattctga acacgttagc gtcgatggac gtgaatatca tacatggcgt
    18001 gaattggcgg atgccttcga actggatatt catgacttca gcgtctctga agtgaatcgt
    18061 tgaattcaca ataaaaaaat cccgaccctg agggtgtcgg gatgaaactt gcttaagcaa
    18121 gaagcactta aaaaattcgt tacaccagga aatctgatgt gttcatcacc ttatctgcaa
    18181 tttttttcgc tgacaagaaa atattcgcgc tatgaatgat taacgctact tatgaataga
    18241 aatgtgacgc gcgtcacccc ttggtggctc tggtgtcaac gggatcgctc ccgaaaaaat
    18301 catctttgat tatttgtgct gttttgagaa tcatcctggt aaggggagta ctgcgcgacc
    18361 atgttttcaa ttcaacaacc actactggtt tttagcgatc ttgatggcac cctgctggac
    18421 agtcatagtt atgactggca accggcagcc ccctggctca gccgtttaca cgaagccaat
    18481 attcccgtca ttctctgtag cagtaaaact ttagcggaaa tgctgtactt gcaaaaaatg
    18541 ttggggctac aaggcttacc gctgattgca gagaatggcg cagtgatcca gcttgctgaa
    18601 caatggcagg atatagacgg ttttccacgc atcatctcag gtattagcca tggcgaaatc
    18661 tgccaggttt taaatacgct acgcgagaaa gaacatttta aattcacgac ttttgatgat
    18721 gtcgacgatg caaccattgc cgaatggacg ggattaagcc gtagctaggc ggcgctgacg
    18781 cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca gtgacgagcg tatggcacaa
    18841 tttatcgctc gtctgaacga actgggcttg cagtttatgc agggtgcgcg cttctggcac
    18901 gtactggatg cctctgccgg aaaagatcag gctgccaatt ggattatcgc gacctatcaa
    18961 caattgtcag gcagacgccc aaccacactt ggcctgggcg atgggccaaa cgatgcgcct
    19021 ttactggagg taatggatta cgcggtgatt gtgaaagggc taaaccgtga aggggtacat
    19081 ctgcatgatg aggatccggc ccgcgtctgg cgaacgcagc gtgaaggacc ggaaggctgg
    19141 cgtgaagggc tggaccattt tttctccgcc cgttaagcgt
//
LOCUS       DADLCQ010000083        17385 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000083 DADLCQ010000000
VERSION     DADLCQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.083"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(42..773)
                     /gene="rlmB"
                     /locus_tag="JQG95_002659"
     CDS             complement(42..773)
                     /gene="rlmB"
                     /locus_tag="JQG95_002659"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY5889163.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(953..3394)
                     /gene="rnr"
                     /locus_tag="JQG95_002660"
     CDS             complement(953..3394)
                     /gene="rnr"
                     /locus_tag="JQG95_002660"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY5889164.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="JQG95_002661"
     CDS             complement(3433..3858)
                     /gene="nsrR"
                     /locus_tag="JQG95_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY5889165.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4063..5361)
                     /gene="purA"
                     /locus_tag="JQG95_002662"
     CDS             complement(4063..5361)
                     /gene="purA"
                     /locus_tag="JQG95_002662"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY5889166.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5465..5662)
                     /locus_tag="JQG95_002663"
     CDS             complement(5465..5662)
                     /locus_tag="JQG95_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY5889167.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="JQG95_002664"
     CDS             complement(5744..6748)
                     /gene="hflC"
                     /locus_tag="JQG95_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY5889168.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="JQG95_002665"
     CDS             complement(6751..8010)
                     /gene="hflK"
                     /locus_tag="JQG95_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY5889169.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="JQG95_002666"
     CDS             complement(8096..9376)
                     /gene="hflX"
                     /locus_tag="JQG95_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY5889170.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="JQG95_002667"
     CDS             complement(9452..9760)
                     /gene="hfq"
                     /locus_tag="JQG95_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY5889171.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="JQG95_002668"
     CDS             complement(9846..10796)
                     /gene="miaA"
                     /locus_tag="JQG95_002668"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY5889172.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMVIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="JQG95_002669"
     CDS             complement(10789..12636)
                     /gene="mutL"
                     /locus_tag="JQG95_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY5889173.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="JQG95_002670"
     CDS             complement(12646..13983)
                     /gene="amiB"
                     /locus_tag="JQG95_002670"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY5889174.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQYEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="JQG95_002671"
     CDS             complement(14002..14463)
                     /gene="tsaE"
                     /locus_tag="JQG95_002671"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY5889175.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="JQG95_002672"
     CDS             complement(14435..15982)
                     /gene="nnr"
                     /locus_tag="JQG95_002672"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY5889176.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15981..17120
                     /gene="queG"
                     /locus_tag="JQG95_002673"
     CDS             15981..17120
                     /gene="queG"
                     /locus_tag="JQG95_002673"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY5889177.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17103..17156)
                     /locus_tag="JQG95_002674"
     CDS             complement(17103..17156)
                     /locus_tag="JQG95_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889178.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3941 a         4959 c         4368 g         4117 t
ORIGIN      
        1 aaaccatcca aatctggatg gcttttcata attcagagga actagctgcg ctgacgaacc
       61 gcttcaaata agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc
      121 attgggatgc tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct
      181 tccgcgccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag tgtatgatcc
      241 gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc
      301 gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc tacttttttc
      361 gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg aacgcctgcg
      421 gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc gtcgaggatc
      481 agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga
      541 cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg
      601 agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc
      661 ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg
      721 gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta
      781 ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat
      841 atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
      901 caaagcgcat tttgtcagca atctaaccct ctttttttaa agagggtatt gatcactctg
      961 ccactttttt cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg
     1021 ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct
     1081 caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc
     1141 cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc
     1201 gcggtgcgcg ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg
     1261 cttcgacgcg aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca
     1321 tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga
     1381 ccagaccatc aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg
     1441 aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt
     1501 cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca
     1561 gttgcaacat ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat
     1621 gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg tcaggataac
     1681 gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc cagaccgaag tgaccacggt
     1741 tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag catggtttgc agcatttctg
     1801 catcaggacg gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt
     1861 taccacccgg cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt
     1921 cggtgctcgg cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac
     1981 gcgccgccga gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac
     2041 gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt
     2101 cgctctcaaa tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt
     2161 tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc
     2221 cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt
     2281 cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac
     2341 acaggcggtc tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca
     2401 tcgggataac ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca
     2461 gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc
     2521 cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat
     2581 caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct tcttccggca
     2641 cttcttcttt cagccccgca acctgttgct caacagcctg cggccagata tacggaattt
     2701 catgggtacg cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt
     2761 cgacgatttt acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga
     2821 ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca
     2881 gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa
     2941 tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt
     3001 cagcacccag cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc
     3061 tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt
     3121 gaccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac
     3181 gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa
     3241 gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt
     3301 cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt
     3361 cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga
     3421 tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa
     3481 atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc
     3541 ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga
     3601 taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg gtttacccag
     3661 gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt
     3721 gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact
     3781 ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt
     3841 gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt
     3901 gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag
     3961 cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca
     4021 ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacgggtc
     4081 gcgcagaatc atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc
     4141 ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc
     4201 tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc
     4261 tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta
     4321 agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa
     4381 gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt
     4441 acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc
     4501 agtttcatca aacagttcgg ccgggaaagg acctgcacct acacgagttg agtaagcttt
     4561 gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc
     4621 accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag
     4681 cgtaccctgc gcaccttcga acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag
     4741 gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag
     4801 aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata
     4861 ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg
     4921 cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac cggtggtgcc
     4981 gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agagcaacgt gataatcaag
     5041 gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc
     5101 cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat
     5161 gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc
     5221 gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc
     5281 ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag
     5341 tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca
     5401 tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt
     5461 ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc
     5521 caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct
     5581 tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg
     5641 ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc
     5701 tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag
     5761 tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat
     5821 tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat
     5881 ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcataa
     5941 tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc
     6001 gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct
     6061 cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga
     6121 tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa
     6181 ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg
     6241 ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt
     6301 cgagggtcag acgaccacgg gaatcggtga cgatatcttt cacgtccagg cgaccaattt
     6361 cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc
     6421 caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagagt
     6481 cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct
     6541 gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag
     6601 gctcataaac caaaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa
     6661 taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga
     6721 tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg
     6781 ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct
     6841 tgctccactg gttgtggagg aagaggctgg cggcagacgc agcagattgc tggcaccgtt
     6901 atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat
     6961 caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat
     7021 cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag
     7081 tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg
     7141 cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata
     7201 cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa
     7261 cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat
     7321 acccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac
     7381 ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc
     7441 gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc
     7501 cggattggtg acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt
     7561 cagcatcaca ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc
     7621 gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa
     7681 gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat
     7741 aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc
     7801 ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt
     7861 tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt
     7921 gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg
     7981 tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat
     8041 ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga
     8101 tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca
     8161 tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa
     8221 gctgataaaa acgactcctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg
     8281 ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg
     8341 tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt
     8401 cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt
     8461 cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg
     8521 catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg
     8581 ccgccaccag atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt
     8641 caccgacatc tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt
     8701 ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg
     8761 tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct
     8821 cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga ttacgcaaca
     8881 aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct
     8941 gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca
     9001 gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa
     9061 ggccggtgcg gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg
     9121 ggctcagggc atggtcaaaa agaacgaccg aagcacccat cgctttgaca gcttccgcaa
     9181 tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg
     9241 taatcacctg caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt
     9301 cttccatatc tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat
     9361 cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct
     9421 ggctccccgt gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg
     9481 cggaagtatt ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt
     9541 tgttactgtg atgagaaacc gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa
     9601 ccatctggct gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc
     9661 cttgcagctt aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg
     9721 cgttcaggaa cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc
     9781 ttatttgtac tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta
     9841 cacattcagc ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt
     9901 ttttcactgt caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc
     9961 gccaactgtc tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct
    10021 tcaaggtaag accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc
    10081 aaatctcctc gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga
    10141 tgaaaacgct gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac
    10201 tgatgcacct gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta
    10261 cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc
    10321 cttgctgccg caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc
    10381 tctgccgcct gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac
    10441 aacccttcca gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc
    10501 cccgccgcgg tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag
    10561 taagcctgcg acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca
    10621 gcgttcggct tcgccgtccc gataaccatc cctttgtaaa taagggcaga atcaacgctt
    10681 atcaactcta ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc
    10741 gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt
    10801 cagggctttt atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt
    10861 cacaagttgc ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga
    10921 ccactgcgca tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc
    10981 gaatacggac tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg
    11041 ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc
    11101 aatacccaat tccgctaggg cagactgcgc tttttctaat gccgattttt cttcgccaga
    11161 aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt
    11221 caattgtgcc tgacgcagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc
    11281 atcgcgctcc agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg
    11341 actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc
    11401 gggcgtttgc aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc
    11461 attcggccag ggggcagccg gacgactgcc tgaggcgggc gcaggagagt agcgcggagc
    11521 taccggctca cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc
    11581 cgggatggca cgcggtgcgg gttgtggttc atcgtccagc ggtagcggcg tttccagttg
    11641 ctgttgtagc acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg
    11701 gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc
    11761 cagatacaac acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg
    11821 gatcgcatga ttgatcaggc gatcgcgcat catgcgacca ttcacgtagc aatactgaat
    11881 ttccgccagt gcgggcgtag tgtgatttgg atcggccacc cagccgcgca gggtgagatc
    11941 gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc
    12001 taagcgccgt tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc
    12061 gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc
    12121 atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt
    12181 gtagaacaga tccagcacct ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt
    12241 cacgtccatg tcgcgtcctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg
    12301 tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc
    12361 caggctgata atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgagc
    12421 cagcgccagc gccagctcat cttttttgat accgcagccg ttatcacgaa tgcggataag
    12481 tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt
    12541 ttccactagt tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg
    12601 gttcgccagt tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt
    12661 gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg
    12721 catcggatgc gcaaggaagt aattgcgcag gcctttgtaa atggcttctg ccagctgttg
    12781 ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc
    12841 gaccagtact gatgggatat ccggcgagcg cagaacgcca aggctggcgt gttctggtcg
    12901 acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata
    12961 ccctacccgc tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc
    13021 agactgactg ttcgccagca catcacccgc cccacccagc agctccgaca gtttctcgta
    13081 ctgctccagc cagctggcca tttcactgtt ggcgcgacgg ttagagagca cccatacgga
    13141 agcgccagtc gcactacggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc
    13201 gttttgctta tgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg
    13261 ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat
    13321 ggcgatggtg acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc
    13381 gccgtgtccg gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc
    13441 tgccggacgc gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt
    13501 aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg
    13561 tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac
    13621 gacagtgtaa ttgctgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag
    13681 atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac
    13741 cagattattg ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc
    13801 gagcgccacg gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact
    13861 caacgttatc cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc
    13921 acccaccggc gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat
    13981 catcccgcca cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga
    14041 ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac
    14101 atccgggtca ggaagaacac ctgtaccttg ttgtggccac tccaccaggc agatggcatc
    14161 gttggcaaaa taatcgcgga tccccataaa ctccagctcc tcgggatcgg caaggcggta
    14221 caaatcaaag tgatagacca ttaagttgtc gagcgtatag ggttcgacca gcgtataagt
    14281 ggggcttttg acattaccct gatgacccag agcctgtaaa aagccccggc taaaggtggt
    14341 tttacctgcg cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc
    14401 tacccgctcg cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat
    14461 catggttttt atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg
    14521 ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg
    14581 cgacacagcc tgcacaggct gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa
    14641 taataccaga gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa
    14701 taatgcctaa agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta
    14761 cgccgccata acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag
    14821 cgacggaaca gcctaacaac cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt
    14881 gacgcttatc gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt
    14941 tgcgaaaatt ctcaactttt tgcagggctt ttttccccca ctcttgctgg cccagaccgg
    15001 gaccaatcac caccacatcg gcccattcca ggctttcggt aagagagtcc atagtcagtt
    15061 catgcaccat caattctggt cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca
    15121 gtactcggac taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc
    15181 ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc gtgatcgcct ttatgcgaag
    15241 tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg
    15301 tctcctgacc tgccagccag ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa
    15361 catcccgcgc ttttccagtg agcaagccta gtttcagcgc aataaaagtg atggtgtgat
    15421 cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat
    15481 caaccgccac aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt
    15541 cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt
    15601 cgggccagac aatattcgaa gcatggatct cgccacccgc gtttaaccat gcttcgcgtg
    15661 ccagcgcggc ctcttccggc aacggtttgt cgctctcctg ggccaacaac gtgacctcaa
    15721 tgccgaccgc tttggccagt cgcgcgacca cgtagccatc gccgccgtta ttaccatgac
    15781 cgcacagcac cagccagtgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg
    15841 cctcgccagc gcgaagcatc agctcataga gtgtgagccc cagcacatct gccgcctcgc
    15901 gttctccgcg gcggatatcg tcggcgtacc agacggtgtg tggtatactt acggggtttt
    15961 tcttcattgt atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa
    16021 ttaaacagtg ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca
    16081 gcgagtccga gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact
    16141 ggatggcacg tcacggtatg ctgcgcgctc gcccccatga gttattaccc ggtacgctgc
    16201 gtgtaatcag cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga
    16261 aaaaccccaa actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc
    16321 tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt
    16381 ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc
    16441 tcggctggac aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt
    16501 taggcgaatt gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg
    16561 gcaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg
    16621 tcgatgctcg ccgctgtatc tcttatctca ccatcgaact tgaaggggcg ctcccggaag
    16681 agttgcgacc gttaatggga aaccgtattt acggttgcga tgactgccag cttatctgcc
    16741 cgtggaatcg ctattcgcaa ctcactacag aagacgattt cagcccgcgt aagccgctac
    16801 acgcaccgga actcattgag ttatttgcct ggagcgaaga gaagttttta aaagtcacgg
    16861 aaggatcggc gattcgccgt ataggtcacc tgcgctggct gcgtaatatc gccgtggcat
    16921 taggcaatgc cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc
    16981 acccacttct cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg
    17041 cgtgcatagt cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag
    17101 ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca
    17161 attcaagacg aataaaatcg tcaagtgatc gactcgacaa aacggaatct tgatttttaa
    17221 cttatttatc aataggttaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DADLCQ010000084        17377 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000084 DADLCQ010000000
VERSION     DADLCQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.084"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            131..574
                     /locus_tag="JQG95_002675"
     CDS             131..574
                     /locus_tag="JQG95_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5889179.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /locus_tag="JQG95_002676"
     CDS             810..1628
                     /locus_tag="JQG95_002676"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY5889180.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQG95_002677"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="JQG95_002677"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY5889181.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQG95_002678"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="JQG95_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY5889182.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQG95_002679"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="JQG95_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5889183.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /locus_tag="JQG95_002680"
     CDS             complement(4319..4468)
                     /locus_tag="JQG95_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY5889184.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="JQG95_002681"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="JQG95_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY5889185.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="JQG95_002682"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="JQG95_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY5889186.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="JQG95_002683"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="JQG95_002683"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY5889187.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="JQG95_002684"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="JQG95_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY5889188.1"
                     /translation="MTQFNLVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="JQG95_002685"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="JQG95_002685"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY5889189.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="JQG95_002686"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="JQG95_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY5889190.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="JQG95_002687"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="JQG95_002687"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY5889191.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQG95_002688"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="JQG95_002688"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY5889192.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="JQG95_002689"
     CDS             13934..14314
                     /locus_tag="JQG95_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889193.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="JQG95_002690"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="JQG95_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY5889194.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /locus_tag="JQG95_002691"
     CDS             complement(15366..16085)
                     /locus_tag="JQG95_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY5889195.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQG95_002692"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="JQG95_002692"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY5889196.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4431 a         4539 c         4316 g         4091 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtctgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccacaagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatctcg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatctatc atcacctgag
    12841 ctggcagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca aacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatatta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctg
//
LOCUS       DADLCQ010000085        17173 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000085 DADLCQ010000000
VERSION     DADLCQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.085"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..185
                     /locus_tag="JQG95_002693"
     CDS             <1..185
                     /locus_tag="JQG95_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY5889197.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
     gene            complement(210..485)
                     /locus_tag="JQG95_002694"
     CDS             complement(210..485)
                     /locus_tag="JQG95_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY5889198.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(506..742)
                     /locus_tag="JQG95_002695"
     CDS             complement(506..742)
                     /locus_tag="JQG95_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY5889199.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="JQG95_002696"
     CDS             complement(860..2299)
                     /gene="rsmF"
                     /locus_tag="JQG95_002696"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY5889200.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASWVKVLHA
                     NISRCGISNVALTHFGGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="JQG95_002697"
                     /pseudo
     CDS             complement(2379..4999)
                     /gene="yebT"
                     /locus_tag="JQG95_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="JQG95_002698"
     CDS             complement(4968..6251)
                     /gene="yebS"
                     /locus_tag="JQG95_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY5889201.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6381..6878
                     /locus_tag="JQG95_002699"
     CDS             6381..6878
                     /locus_tag="JQG95_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY5889202.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6975..7673
                     /gene="proQ"
                     /locus_tag="JQG95_002700"
     CDS             6975..7673
                     /gene="proQ"
                     /locus_tag="JQG95_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY5889203.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7693..9741
                     /gene="prc"
                     /locus_tag="JQG95_002701"
     CDS             7693..9741
                     /gene="prc"
                     /locus_tag="JQG95_002701"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY5889204.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9933..10814
                     /gene="htpX"
                     /locus_tag="JQG95_002702"
     CDS             9933..10814
                     /gene="htpX"
                     /locus_tag="JQG95_002702"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY5889205.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10860..12233)
                     /locus_tag="JQG95_002703"
     CDS             complement(10860..12233)
                     /locus_tag="JQG95_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5889206.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12410..13201
                     /gene="kdgR"
                     /locus_tag="JQG95_002704"
     CDS             12410..13201
                     /gene="kdgR"
                     /locus_tag="JQG95_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY5889207.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13344..13583)
                     /locus_tag="JQG95_002705"
     CDS             complement(13344..13583)
                     /locus_tag="JQG95_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889208.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13742..13885
                     /gene="mgrB"
                     /locus_tag="JQG95_002706"
     CDS             13742..13885
                     /gene="mgrB"
                     /locus_tag="JQG95_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY5889209.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13960..14247
                     /locus_tag="JQG95_002707"
     CDS             13960..14247
                     /locus_tag="JQG95_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889210.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14826..14969
                     /locus_tag="JQG95_002708"
     CDS             14826..14969
                     /locus_tag="JQG95_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY5889211.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14982..15191
                     /gene="cspE"
                     /locus_tag="JQG95_002709"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14982..15191
                     /gene="cspE"
                     /locus_tag="JQG95_002709"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY5889212.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15357..16166
                     /gene="rlmA"
                     /locus_tag="JQG95_002710"
     CDS             15357..16166
                     /gene="rlmA"
                     /locus_tag="JQG95_002710"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY5889213.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="JQG95_002711"
     CDS             complement(16163..16729)
                     /gene="mntP"
                     /locus_tag="JQG95_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY5889214.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIITTALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4532 a         4155 c         4337 g         4149 t
ORIGIN      
        1 atgaatggca aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg
       61 aacgcccaaa agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt
      121 tgcgacatcg cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg
      181 gctaactgac tcttgtgcaa ctgcaataat taaaaatcac catactcctg tgctggtcgc
      241 cagaaaccat ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg
      301 ctcatagaat aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa
      361 ccgccatcta ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt
      421 gccgtaccgt taagtttagg agagatttta caacggggga ataaccagga caaataaccc
      481 gccatgccga cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt
      541 gatcgtaacg ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg
      601 tttcagcatc ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg
      661 ttaacgttcg atcacccggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc
      721 ctatgcgcac actggttttc atcttcgtgc tctccattta gctgctgatt gtagtacgac
      781 cagtccgatc gcacaatttt tcagcctagt tgaggccggc gaatgtgcca gtcaaaaagt
      841 gcgctttttt tgtgcgctgt catgcgttac tggtaaaaag tttcccgtcc cgcaccagtt
      901 cacgcgggta gctgtttttc agtcgcgaac caatccgttt ggctaaacca atcggctgat
      961 gctggaaagt aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc
     1021 cgcgatacca ctcctccgct tcctgcggtg tcagttcaaa agcgtttaca ttgtcggggg
     1081 aggcatgggc aataaccgct tcatgctgcc agcgataacc tttgttatgc gtttcggcaa
     1141 gtttaatccc taagcgagaa aatcggactt taccgatcag ggcttcaatg cctaccggga
     1201 acaaccacag ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa
     1261 cacttgcagc cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga
     1321 aattgcccac tttgtatttt ggggcgggta aggcggggat cgcctgagtt ttacgcagac
     1381 gagcaacgaa gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aaaccttctt
     1441 cggtcagcgc cttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctacttcgt
     1501 cggggtaagt ctctttcagc caccggcaaa cggcttcgtt ttcttcccgg tttaaggtac
     1561 aggttgagta aaccagcgta ccgccaggac gtaatgcatg aaaggcgctg tcaatcagtt
     1621 cccgttgggt agctgcgatt tcctgattgc tttcgggtga ccagtttttt agcgcatcgg
     1681 gatctttacg caccacgcct tcgccggagc agggagcgtc cagcaaaatg gcatcaaaca
     1741 tttctggcac tgccgcacca aacacgcggc caccaaaatg tgtgagcgca acattactga
     1801 tgccacagcg gctgatattg gcatgtaaca cttttaccca actggcggaa aactcattgg
     1861 caaggattgc cccttcgtta ttcatccgcg cggcaatttg cgtcgttttt gagcctggcg
     1921 cagcagcgac atccatcacc cgctgtggtg cattaccgtc agcaaacaag gcggcgacgg
     1981 gcaacattga actggcttcc tgaatataaa acaggccact taaatgctcg gtggtactac
     2041 ccaatggcaa tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa
     2101 ttggcgtaag cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt
     2161 tcagcgtatt aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat
     2221 caaatgagag cgtcgaaggc atagcttcgc gcatttgtgt cagaaacgcg tccgggaaat
     2281 aaacggtgtg ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc
     2341 ataagcgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc
     2401 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcttgggct
     2461 ttcggtgcaa gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaattgg
     2521 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     2581 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     2641 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2701 agaccacgga acagcacagg cgtaccgatg cctaacgaac cggcttccgg cgcttcaaca
     2761 ataatgctta aaccatctag gtaacgcgaa tcagtaatgg tggcctcttc taattcaaag
     2821 tcgcgacgag gattgccacg gcctggttcg acgttgatat acggctggag gatagtatct
     2881 agatgctcaa cgccagctgc cgagatttgc ggtgtgacca ctgagaagcg cgtaccaccg
     2941 cgagcaaagg tctggacata ttccggatag agcaccgcct ttgcctgtac ttcattgcgt
     3001 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     3061 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgcct gccaaccgca
     3121 cgggccgctg tttcggaagc atacagaatt cgcttgtcgc ctttacgctg actggcgctg
     3181 gcaccactga ggttatcgaa gctaatggcc ccctttaatg ctctggagag cggggatgcc
     3241 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     3301 ctgttgctgg tcaaaaggtt gcgatattcc ggtttaatgt gcagatcgat atcgaacgcg
     3361 ttagctcgcg gacgcacggt aataacttca ccaacttcaa atttacggta cagcactacc
     3421 gatcctgcct gcacatccgg cagcgtcttt gcactcaaac tcacggttgt ggtgggtaaa
     3481 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     3541 atctcgcctt tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     3601 gcaccgagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3661 aatttgctat cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg
     3721 acgcctttgc tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg
     3781 agcggctgac ccgcatcaat accgtaactt tccggtgctg tcagggtcag tgtcagaacg
     3841 tccggctctt gcagcagggc tttttcgccc ggcacaacaa cgaactcttt gcgtggctcg
     3901 ccatcgccgg gcaccaactc aaaggttttt ccggtcagca gggcgctgag attagcatcg
     3961 ctaagggata atttcgggtt gcgtaattcg atgcgggtat tttcacgaag cagggatcaa
     4021 cggtcatttc cccagtgact ttaccaccag gatttaaatc cagtttagtc agctgtccga
     4081 cttccagccc ctgatacatt aacggcgtcg agtcggcggt taatcctgct ccactcggca
     4141 gttccagttt tattattact ccacgctggc tgtgggccag atcttcatac agaccaaagg
     4201 tatcttccgc ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa
     4261 ccagtgccgc cagactttcc agtttcacct tcgcgccact gatactgacg ttggcatcaa
     4321 cgccggaaac gtttcagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga
     4381 tcaggacatc aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc
     4441 ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agagcggggg
     4501 cttgcaggtg gatcatcaga tcgccattgt ccagccgata tttcggttgg gtatcgagcg
     4561 cgacaaagtg atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga
     4621 cgagggcgtc cagcccggag acacctgcca acgatgcttt tggcgtcatc agccagaact
     4681 gagtctcttc gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct
     4741 tacgaagatc gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac
     4801 gaacaggcgt acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat
     4861 taccccggtc ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag
     4921 gcagcagcca gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag
     4981 cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca
     5041 agaatagtca aaattacggc agcgccaaaa taaaacgcag ccggtcccat agtaaaagcg
     5101 aggatctgat cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc
     5161 gaccagcgtc caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc
     5221 tggcatttaa aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt
     5281 accagaatac tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac
     5341 ataattccgg aaagaatcgt atcttcctgc cggccaccat tcagataaat gatagaaata
     5401 ggcaataggt tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt
     5461 tgcaaactat gacgacgacg caggcgtagc gggatatggc aacgcgggca gagaccacgc
     5521 tgatcaggat agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc
     5581 gtagcggggc gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac
     5641 gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca agccgacacc cgcctggata
     5701 tgggcataat cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc
     5761 attacccact ctttaagtcg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt
     5821 cggttaccaa accataaata tgctatggag gacaccagaa tgaggggggc accaataacg
     5881 cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt tggtcatttg ccagatgcct
     5941 tgcatgacgt tggcgtcaat acgaatgcct aacagccaga tatgcaacag cggttcgccc
     6001 caggcaaacg gcataagcaa cagcatggtg aaggccattg ccgccaggcg cgttagcgac
     6061 cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac aataggcact ttgatgagaa
     6121 tttatctcgg gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc
     6181 ggcagttcct cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg
     6241 ttaagagcca tgacgtgcca aatgtaataa gaattaacat taatcttaac ccatgatggg
     6301 aatgatcttg agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa
     6361 gacaaccaag tgattaagta atgaacaaaa cagaatttta cgcggattta aatcgcgact
     6421 ttaacgcgct gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt
     6481 tgttatacga gcgtctcact gacgttaact gggcaggttt ttatttgctt gaggacgata
     6541 cactggtact cggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg
     6601 gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt
     6661 ttgacgggca tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg
     6721 tgaaaaatca gattattggt gttctcgaca tcgatagtac cgtcttcgct cgctttacag
     6781 acgaggacga gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga
     6841 cggattacaa aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta
     6901 ctggtagcgt cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt
     6961 aatcaggaaa tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt
     7021 ttctggccga acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa
     7081 ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc
     7141 aattacgatc cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac
     7201 ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg
     7261 tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg
     7321 aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc
     7381 gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac
     7441 ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac
     7501 agcacacccc ggtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag
     7561 cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc
     7621 agctgaattc gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag
     7681 gccgggccag gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata
     7741 gcaggccaga ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag
     7801 gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat
     7861 tatcgccagt tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat
     7921 ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag
     7981 aaaaccgagt taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac
     8041 aatctggcgc aaaagcgccg ttttgaacgt taccagtacg ctttgtcggt actggaaaag
     8101 ccgatggatt tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg
     8161 aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag tcaaattcga cgagttaagc
     8221 ctgaagctga caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa
     8281 tttgccattc gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg
     8341 gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt ccccgcgtaa taccgaacag
     8401 ttcaacactg aaatgagttt gtcactggaa ggtattggcg cagtgctgca aatggatgat
     8461 gactacaccg ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc
     8521 agcgttggtg acaaaattgt cggtgttggt caaacaggca agccgatggt tgatgtgatt
     8581 ggctggcgtc ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt
     8641 ctggaaattt tacctgctgg taaagggacc aagacccgta ctgtaacgtt gacccgtgaa
     8701 cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa
     8761 gtcggcgtgc tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa
     8821 ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc
     8881 ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt
     8941 gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg
     9001 ttctataaag gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc
     9061 tttgccgcgg caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt
     9121 aaaggcaccg ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct
     9181 gaatggccag cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc
     9241 ggcagtacgc aacgtaaagg cgtaacacca gacatcatca tgccgacggg taatgaagaa
     9301 acggaaacgg gtgagaaatt cgaagataac gcgctgccgt gggatagcat cgatgccgcg
     9361 acttatgtga aatcaggaga tttaactgcc tttgaaccgg agctgctgaa ggaacataat
     9421 gcgcgtatcg cgaaagatcc tgagttccag aacatcatga aggatatcgc gcgcttcaac
     9481 gctatgaagg acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat
     9541 aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg agaaggtaaa
     9601 ccggagttga agaagctgga tgatctatcg aaagattacc aggagccgga tccttatctg
     9661 gatgagacgg tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa
     9721 caacccgctc ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac
     9781 agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact
     9841 tacgcactat ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt
     9901 gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa
     9961 cgaacctggc cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga
    10021 gcagcgttca ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt
    10081 cgcttctgat gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac
    10141 cgcgtaacga aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcgg
    10201 ggatcgctat gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg
    10261 gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc
    10321 cggatgaagc cgaggcggta attgctcacg aaatcagcca catcgccaat ggtgatatgg
    10381 tcaccatgac gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc
    10441 tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg
    10501 gcaacccgct gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg
    10561 cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg
    10621 caaaactggt tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg
    10681 aaccgcaaga agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca
    10741 gtgagttgtt catgactcac ccgccgctgg ataaacgtat tgaagctctg cgtacgggtg
    10801 aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt cctgatcgac gcgctttttt
    10861 tatgccctgg ctcgtggctg agtgatacgt aaaccactga cacaggcagc aatcactgcc
    10921 agaatagccg cagccatcag cgagacgtgc gtaccattat caccaaactg atttagcatc
    10981 agcgccacca gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca
    11041 ctggccccac cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac
    11101 tggaataagc caaatccagc gccacataag atcatcggcc agataatatt gatatccgca
    11161 ggtgacgcgg gcagcagaac cagggaaaaa agccccgtag ccatgatgaa caaccctaaa
    11221 gcacccagca atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc
    11281 atcgttgcta acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc
    11341 acggtttgca ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa
    11401 acagatgtgc aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc
    11461 ggtacgggaa gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc
    11521 agttccgcag caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata
    11581 agcaggccga aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg
    11641 gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac
    11701 ggtacgttga ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt
    11761 gtcggcccgg cagcagaaga gacggcaaca ataaacgagt ttatgcccat ccctctcccc
    11821 agaaaacgtt gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca
    11881 ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg
    11941 cagaacagtg aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca
    12001 aacatatcgc ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg
    12061 ttcactaccc agatggaact ggctggtgtg gcatgaaggt ccgtggcaat tgttggcagg
    12121 gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa taccaatcac aatggttaat
    12181 atcgcaccgt atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt
    12241 ttatatcttt ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat
    12301 gtcgcagaat tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat
    12361 aataaaaaac agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc
    12421 agatctggat aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca
    12481 ggcgctgggt gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc
    12541 aaaaagcacc gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga
    12601 aggggagtcg gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt
    12661 acaaaacgtc gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac
    12721 caaagaaact atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat
    12781 tgactctatg tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag
    12841 caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc gatgaagtga agcaaattct
    12901 tgagggcgtg gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt
    12961 acccgttctg gaccaggtgc gcgagcaggg gtatggagaa gataacgaag agcaggaaga
    13021 agggctgcaa tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt
    13081 gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat
    13141 gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg
    13201 atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa
    13261 ataaagttga aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt
    13321 gagaatctgg ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac
    13381 agtgggcaat ctgttatctc caagaaacca cttttagtgt gcaagtattg taccgtgctg
    13441 gtgcctctgg cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt
    13501 ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata
    13561 actgtgcgaa tgatgaatcg cattacaatc tcttctcttt ttatgttcgc ttaatcgtag
    13621 cggcaatatg cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta
    13681 ggcgctgttt aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg
    13741 agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg
    13801 ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc
    13861 cattaaccag tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc
    13921 agcagtggta gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca
    13981 ggcgtgatga acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc
    14041 ttgtggtttt ttatcaatcg tgccagttca cggactaacg aacagattga actgcttgag
    14101 gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac
    14161 gaaccagaga aagcagataa aaagaccatt gagagtcaaa aatcggttga tgacgaagat
    14221 attattcgcc tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga
    14281 agggttactc aattcatcct gattatacgc actgtcatgg tgcaactgct tcaaattaaa
    14341 actctggtct gacttcaaat aattatcata ttatttatta gcgaaacgtt tctctcttga
    14401 tttttttgct cactcatcaa tttttcttat tttaaattta caatcccttt ggggattgac
    14461 ttctctttag ggtaattgat agccgttaac tgactgtttt atgagaaaaa gtgatataac
    14521 tttttattca ttgcatagca gaaaatgtga tattgcacgc actatgtaat aacttctccc
    14581 tctggcctgg aacaactgaa cttattgaac tatgttagaa actacgccag tttaagtatc
    14641 tgcctgaact ggcaaggtta agcacaatga tatatcggcg cgtattccgt tgcataagtg
    14701 tgcaaaaaaa gtggaagacg tatcgagatt tgtgcgtctg atcgagacat gtttaaaaat
    14761 ggcttgccat aattaacgtt gtatgtgata aaagatttcg ggttaaacga ggtacagttc
    14821 tgtttatgtg tggcattttc agtaaagaag tcctgagtaa acacgttgac gttgaatacc
    14881 gcttctctgc cgagccttat attggtgcct catgcagtaa tgtgtcagtt ttatctatgt
    14941 tatgcctgcg ggcgaagaaa acaatctaag gaatttttca aatggcaaag attaaaggtc
    15001 aggttaagtg gttcaacgag tctaaaggtt ttggcttcat tactccggct gatggcagca
    15061 aagatgtgtt cgtacacttc tccgctatcc agggtaatgg cttcaaaact ctggctgaag
    15121 gtcagaacgt tgagttcgaa attcaggacg gccagaaagg tccggcagct gttaacgtaa
    15181 cagctatctg atcgaatcca ctgatctgaa gtgtgaatac gcttcaatct cgctataaag
    15241 cctcgtcgaa tgcgaggctt tttactatgc tttatcttcg ctcctggcgt tcggatattt
    15301 gcccgccgcg tgattcgcgt tacacttgcg gcctttagta tcctgctgga gttgtcatgt
    15361 cgttttcctg tccactttgc catcagcctc tttcgcgtga aaaaaacagc tatatctgtc
    15421 cccaacgaca tcagtttgat atggcgaaag aagggtatgt caatctgctg cccgttcagc
    15481 ataaacggtc tcgtgatccg ggcgacagcg cggaaatgat gcaagcacgc cgcgcattct
    15541 tagatgccgg acattatcag ccgctgcgtg atgcaattgt cgcccaactg cgggaacggc
    15601 ttgatgaaaa ggccacggcg gtgctggata ttggctgtgg tgaagggtat tacacacacg
    15661 catttgccga tgcgttgccc gaaatcacca cgtttggcct ggatgtctcg aaggtagcga
    15721 taaaagcggc ggcgaaacgc tatccgcagg tcactctttg tgtcgcttcc agccaccgtt
    15781 tgccgttttc cgataccagt atggacgcca taatacgtat ttacgcgccg tgtaaagcag
    15841 aagaattagc acgagtagtg aagcccggcg gctgggtcat tactgccacg ccgggaccgc
    15901 gacatttgat ggagctgaag gggctgattt acaatgaagt acatcttcat gcacctcatg
    15961 cagaacaact ggaaggtttt acattacagc agagtgcgga gttgtgttat ccgatgcgtc
    16021 ttcgcggtga tgaagccgtc gcattattgc agatgacgcc gtttgcctgg cgtgcgaagc
    16081 cagaagtctg gcaaacactg gcagcaaaag aagtgttcga ctgccagacg gactttaata
    16141 ttcacctctg gcagcgttct tattaaccgt ggaagtgcgt ccagaggatc tggacgccga
    16201 tgccgatcag caccagcccg ccgagaattt ccgctttttt cccaataatt gagccgataa
    16261 agcgaccaac catcatccct aatgtcgaca taatcaaggt tgcacaacca atggccaatg
    16321 cggtcgtgat aatgttgacc tgaaggaaag caagaccaac acccacagcc atggcatcca
    16381 ggctggtggc aatcgcggtg gttaccagta gccagaaacc gtgccgacgg cgcggctctt
    16441 catcttcatc atctgcgcca cgaaaaccct caataatcat tcgcccgccg aggaatatca
    16501 gcagcacaaa cgcaatccag tgattccatt caaggacaaa ccggctggct aacatgccca
    16561 ttccccagcc gatcagcggc gtcagggttt cgacggcacc aaaaataagg ccggttcgca
    16621 atgcttcaga aaatttcggt ttatggaggg gggcaccttt accgattgat gcagcaaatg
    16681 catccatcga cataccaaac gcaagaagaa cagtagcagt gatattcatg acaacgtcct
    16741 gaccggggta tccataaaac acatcatcgc ccccagcaaa catgcataca tgaacaacaa
    16801 gccgcgatgg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
    16861 gactgaaagt cttcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
    16921 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
    16981 aatataaaac aactgttgga atatattatt tcattgcttt attgataacg attttcattt
    17041 atgtttatat atcaatgaaa cgttaaaata aataacaaat aagggaagag ataatatagc
    17101 ctaagctatg tctgattatt aaaaatacac taaacatagc tttggctaaa ttcataagtt
    17161 attgagttga tac
//
LOCUS       DADLCQ010000086        17052 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000086 DADLCQ010000000
VERSION     DADLCQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.086"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..213)
                     /locus_tag="JQG95_002712"
     CDS             complement(<1..213)
                     /locus_tag="JQG95_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY5889215.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JQG95_002713"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JQG95_002713"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY5889216.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JQG95_002714"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JQG95_002714"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY5889217.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JQG95_002715"
     CDS             2894..3634
                     /locus_tag="JQG95_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5889218.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JQG95_002716"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JQG95_002716"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY5889219.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="JQG95_002717"
     CDS             complement(4192..4794)
                     /locus_tag="JQG95_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY5889220.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4863..4928
                     /locus_tag="JQG95_002718"
     CDS             4863..4928
                     /locus_tag="JQG95_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889221.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5067..5678
                     /gene="ruvA"
                     /locus_tag="JQG95_002719"
     CDS             5067..5678
                     /gene="ruvA"
                     /locus_tag="JQG95_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY5889222.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5687..6697
                     /gene="ruvB"
                     /locus_tag="JQG95_002720"
     CDS             5687..6697
                     /gene="ruvB"
                     /locus_tag="JQG95_002720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY5889223.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="JQG95_002721"
     CDS             complement(6844..7629)
                     /gene="znuB"
                     /locus_tag="JQG95_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY5889224.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="JQG95_002722"
     CDS             complement(7626..8381)
                     /gene="znuC"
                     /locus_tag="JQG95_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY5889225.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8460..9392
                     /gene="znuA"
                     /locus_tag="JQG95_002723"
     CDS             8460..9392
                     /gene="znuA"
                     /locus_tag="JQG95_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY5889226.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9408..10730
                     /gene="mepM"
                     /locus_tag="JQG95_002724"
     CDS             9408..10730
                     /gene="mepM"
                     /locus_tag="JQG95_002724"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY5889227.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10850..11821
                     /gene="lpxM"
                     /locus_tag="JQG95_002725"
                     /gene_synonym="msbB"
     CDS             10850..11821
                     /gene="lpxM"
                     /locus_tag="JQG95_002725"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5889228.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="JQG95_002726"
     CDS             complement(11952..13394)
                     /gene="pyk"
                     /locus_tag="JQG95_002726"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY5889229.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13522..14391)
                     /locus_tag="JQG95_002727"
     CDS             complement(13522..14391)
                     /locus_tag="JQG95_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY5889230.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14728..16203
                     /gene="zwf"
                     /locus_tag="JQG95_002728"
     CDS             14728..16203
                     /gene="zwf"
                     /locus_tag="JQG95_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY5889231.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16438..>17052
                     /locus_tag="JQG95_002729"
     CDS             16438..>17052
                     /locus_tag="JQG95_002729"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="catalyzes the formation of
                     2-dehydro-3-deoxy-6-phospho-D-gluconate from
                     6-phospho-D-gluconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY5889232.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRM"
BASE COUNT         4352 a         4205 c         4434 g         4061 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagatacg aatatacgta atagcataaa aaattatttt tcaggagtcg
     4861 caatgtatat ttttattacg catttcttca ctgaatatgt aatattcaaa tatttgcttc
     4921 caatataacc tgttgaataa attatactgt gccatttttc agatcatcgg gacacctcgc
     4981 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatc
     5041 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
     5101 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
     5161 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
     5221 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt
     5281 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggccctggcg atcctctccg
     5341 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
     5401 aactgccggg tattggcaaa aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat
     5461 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
     5521 ctagcccggc gaccgacgat gctgaacaag aagcagttgc cgcgctggtg gcgctaggct
     5581 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac accagcagtg
     5641 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
     5701 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
     5761 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
     5821 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
     5881 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
     5941 gacttctggt ccggtgctgg aaaaggcggg cgatttggct gcgatgctca ctaaccttga
     6001 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
     6061 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
     6121 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
     6181 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
     6241 ggtgccggat ctgcaatata tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag
     6301 tgatgacggt gcgcaggaag tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg
     6361 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     6421 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     6481 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
     6541 cctggcggca gccattggcg aagaacgtga aaccattgaa gatgtactgg aaccttattt
     6601 gattcagcaa ggctttttgc agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg
     6661 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     6721 atccgacact ggcagtgtgt ctgatgcgac actgttcgcg tcttatcagg ccagccagag
     6781 atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat
     6841 agattagctg gcctgctttt tcatcatact gataataaat aacagtgccg cacatagcac
     6901 caccgacgga cccgccggcg tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc
     6961 caccatcccc accaaaacag cgacaccagc catctgttcc ggcgtacggg caaagcgacg
     7021 cgcagtagct gcaggaataa tcagcaacga agtaataatc aacgcaccga caaatttcat
     7081 cgctacacca atcgtcaatg ccgtcaccag catcaacaac aatttcactt gctgtaattt
     7141 cacaccatca acaaacgcca gatccgggct aatcgtcatc gacagcaaat tgcgccattg
     7201 ccagaacaaa atagccacca cgatgaccac gccaatcgca atagagatga gatcttctgg
     7261 cgtcactgcc agtaaatcac cgaacaggta agccatcaaa tcaacacgaa tgttagacat
     7321 cagactaacg accactaggc ccagcgacag ggcactgtgc gccataatcc ctaataacgt
     7381 gtcgatcgcc agctgtggac gcttctccag ccataccaga ccgcccgcca gcagcagcgt
     7441 aacggcaatc accgcataga atggattcac gtccagcaac aaaccaaacg cgacaccaag
     7501 taatgaggca tgagccagcg tatcaccgaa ataagacata cgacgccaga ctacaaacga
     7561 acccagcgga cccgcggcac aggcgagcat gatcccggct aaccaaccgg gaaataataa
     7621 ttcaatcatg agcgatcatt tccccgacgc aaaacaattc gtccctgtaa atcgtgacga
     7681 tgattatgat gatggcgata gatacccagt tgttcagcac cacgaggacc aaacattgaa
     7741 ataaactccg gatgcaggga aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc
     7801 aggcacagca cttcatcggt ttttgccatc accagatgca gatcgtgaga aaccattaaa
     7861 acgccacaat ccagttcacg acgcaactgg tcaataaggt catataacgc cacttggcca
     7921 tttacatcca cgccttgagt gggttcatcc agcactaata attgcgggca atttaacaat
     7981 gctcgcgcta atagtacacg ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta
     8041 atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca
     8101 gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg tggtgtcgag atacagcttc
     8161 tgcggtacat agccgatgcg cagttttccg ttgcgcttga taaccccttc atcgggtgtt
     8221 accagcccga gcactacccg taccagtgtc gacttacctg cgccatttgg cccaagtaaa
     8281 gtcaaaattt ttccaggttt aagttccagc gacacatcag agaggacgcg gcgttggcca
     8341 aaagaaaccg agacattttc cagggaaacc agacttgtca tgttaatttt agtcttgcag
     8401 tagtcatgaa atgttataat atcacacttc tcatattcat tacgatgatt ggtcgcatta
     8461 tgttacataa aaaaacgctt cttttcgcag cattatccgc cgctctctgg ggaggtgcaa
     8521 cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg
     8581 ccattgctga tggtgtaaca gaaacggagg ttttactgcc tgacggcgct tcagaacatg
     8641 attattcact gcgtccatcg gatgtaaaac gcttacagaa cgcggactta gtcgtttggg
     8701 ttggcccgga gatggaagcg tttatgcaaa aaccggtaag caaattacca gaagcgaagc
     8761 aggtaacgat tgcgcagctt gaagatgtga aaccgctact gatgaaaagt attcacggcg
     8821 atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca
     8881 acatgcatct ttggctttcc ccagagatag cgcgggctac agcggttgca atccatggaa
     8941 aattagtgga acttatgccg caaagtcgag ccaaacttga cgccaacctg aaggattttg
     9001 aggcacaatt ggcctcaacc gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga
     9061 aaggttattt cgtttttcac gatgcttacg gctatttcga aaaacagttc ggactgacac
     9121 cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa
     9181 taagaacaca gttggttgag caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc
     9241 cagcggtcgt tgagagcgtc gcacgaggga catccgttcg tatgggaacg ttggatcccc
     9301 tggggacgaa tatcaaactg ggtaaaacaa gctattcaga attcctgagt caattagcca
     9361 accagtatgc gagctgcctg aaaggagatt aatgaggaag tgattacgtg caacagatag
     9421 cccgctctgt cgccttggcg tttaataatt taccgcgacc acaccgcgtt atgttggggt
     9481 cgctcaccgt tcttactctg gccgtcgctg tctggcggcc ctatgtttat caccgtgatg
     9541 ccacgccaat tgtcaaaacc attgagctgg aacagaacga aattcgttcg ctcttacctg
     9601 aagccagtga gccgattgat caagctgcac aagaagatga agccattccc caggacgaac
     9661 tggatgacaa aatcgccggt gaagcgggcg tgcatgaata tgttgtttcc actggcgata
     9721 cgctaagcag cattctcaat cagtatggta ttgatatggg tgatatcacc caacttgctg
     9781 ctgccgacaa agaattgcgt aacctgaaaa tcggtcaaca actctcctgg acattaaccg
     9841 cggacggcga actgcagcgc ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg
     9901 accgtactgc cgctaacggt tttaaaatga ccagcgaaat gcagcaagga gagtgggtta
     9961 acaatctgct gaaaggtacc gtcggaggga gctttgttgc cagcgccaga aacgccggtt
    10021 taaccagcgc cgaagtgagc gcagtgatta aagccatgca gtggcaaatg gatttccgca
    10081 aactgaaaaa aggcgatgaa tttgcggtat taatgtctcg agaaatgctt gatggtaaac
    10141 gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa
    10201 tccgcgctga ggatggcaaa ttctacgatc gtaacggtac tggtctggcg aaaggattct
    10261 tgcgattccc gacggcgaaa cagttccgta tctcgtctaa ctttaacccg cgtcgaacta
    10321 atccggtgac aggtcgcgtt gcaccacaca gaggtgttga tttcgccatg ccacaaggta
    10381 cgccagtgct ttcagtgggt gacggtgaag tggtggttgc caaacgcagt ggcgcagcag
    10441 gttattatgt ggctattcgt catggtcgca gctacaccac gcgttatatg cacttgcgca
    10501 agattctggt gaaaccggga cagaaggtga aacgtggcga ccgtatcgcg ctttccggta
    10561 ataccggacg ttcaactggg ccgcatctgc actatgaagt atggataaac cagcaggccg
    10621 taaacccgct gacggcaaaa ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg
    10681 aattcctggc gcaggccaaa gagattgtgc cgcagctacg gtttgattaa ttaacatcca
    10741 ttcgcagccg gtacgcagtc agtaccggct ttttttattt ggtgcggggc aagttgtgcc
    10801 gctacactat caccagattg atttttgcct tatccgaaac tggaaaagca tggaaacgaa
    10861 aaaaaataat agcgaataca ttcctgagtt tgataaatcc tttcgccacc cgcgctactg
    10921 gggagcatgg ctgggtgtag cagcgatggc gggtatcgct ttaacgccgc caaagttccg
    10981 tgatcccatt ctggcacggc tgggacgttt tgccggacga ctgggaaaaa gctcacgccg
    11041 tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt agtgaagctg aacgcgaagc
    11101 gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc
    11161 aatacgcggg ccggagaaaa ttcagccgcg cgttggctgg caagggctgg agatcatcga
    11221 agagatgcgg cgtaataacg agaaagttat ctttctggtg ccgcacggtt gggccgtcga
    11281 tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg gcagcgatgt tccataatca
    11341 gggcaacccg gtttttgatt atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct
    11401 gcatgcgaga aatgacggta ttaaaccatt catccagtcg gtacgtcagg ggtactgggg
    11461 atattattta cccgatcagg atcatggccc agagcacagc gaatttgtgg atttctttgc
    11521 cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt
    11581 tgtaccgctg tttccgattt atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc
    11641 accgatggat gatctgttag aggcggatga tcatacgatt gagcggcgga tgaatgaaga
    11701 agtcgagatt tttgttggtc cgcgaccaga acaatacacc tggatactaa aattgctgaa
    11761 aactcgcaaa ccgggcgaaa tccagccgta taagcgcaaa gatctttatc ccatcaaata
    11821 aaaaagcctc tcgcgaggag aggccttcgc ctgatgataa gttcaagttt gcttcagaat
    11881 attcgaaatc tgttgaacta tcattgaact ataggccgga tgtggcgttt tcgccgcatc
    11941 cggcaacgta cttactctac cgttaaaata cgcgtggtat tagtagaacc cacggtactc
    12001 atcacgtcgc cttgggtgac aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc
    12061 agcagattaa ccgcttcgct ggcagctgct acgccgtcat tagcgctatc aaagtgcacc
    12121 ggcgtaacgc cacgatagag agcagtcagg ttcagcgtac gttcatggcg cgacatggcg
    12181 aaaattggta gaccagagct gatacgggag gtcatcagcg cggtacgacc cgattcggtc
    12241 atggtaatga tcgccgtaac gcccttcagg tggttagctg cgtacattgc cgacatggca
    12301 atagcttctt ccacattgtc gaactgaacg tccagacggt gtttagaaac gttgatgctc
    12361 gggatttttt ccgcacccag gcaaacgcgc gccatggctg caacagtttc tgacggatac
    12421 tgcccagcgg cagtttctgc agacagcatc acagcgtcag taccatccag aacggcgttt
    12481 gctacgtcca tgacttctgc acgcgtcggc atcgggttag taatcattga ctccatcatc
    12541 tgggtcgccg tgattaccgc tcggtttagc tgacgcgcac gacggatcaa cgctttctga
    12601 atgccgacca gttccgggtc gccaatttcc acaccgaggt cgccacgtgc aaccattacc
    12661 acgtcagagg cgaggatgat gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt
    12721 tcaaccttgg caacaatttt cgcatcacat cctgcatcgc gtgccaggcg acgggcatag
    12781 ttcagatctt cgccacagcg tgggaaggag acagccaggt aatctacgcc aatcaacgcc
    12841 gcagtcttaa tgtctgcttt gtctttttcg gtcagcgctt cagccgacaa accgccgcca
    12901 agtttgttga tacctttatt gttggagagg ggaccaccga cggtgacttc ggtgaacact
    12961 ttcatgccct gaacttccag tacttttaac tggacgcgac catcgtccag cagcaggatg
    13021 tcaccaggca cgacgtcagc aggcaggcct ttgtagtcga taccgacttt ttctttgtcg
    13081 ccttcacctt tacccaggtt ggcgtcgagc aggaatttat ccccaatatt gaggaaaact
    13141 ttgccttctt taaaggtgga tacacggatt ttgggcccct ggaggtcacc cagaatagcc
    13201 acatgacgcc ccagttttgc ggcaatctca cgaactttat ccgcgcgcat tttgtgatct
    13261 tcaggcgagc cgtgagaaaa gttcatacgt acaacgttgg cacccgccgc gataactttt
    13321 tcaagattat tatcgcgatc tgttgctggg cctaacgtgg taacgatttt tgttctgcga
    13381 agccttttgg acatgtaata ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa
    13441 atccgaatga aataacgccg cgatggcgat aaattaatct cattcggatg tcgtataaga
    13501 ttaggacagt gacagtcgct tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa
    13561 acgcgattcc ttcagcgctt ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg
    13621 tcgcaaagta aatcctgtcg ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac
    13681 catgggcata taaatgtcag tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc
    13741 ccgggcgagc ggggtacccg cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc
    13801 cagctgcgcc agctcgacca gattttttgt tcttcctgtg tgagaaatca gcaccaccac
    13861 gtctccgtcg ctacaattca tacaactcat gcgttgcagc acgatatcat cggagtacac
    13921 caccggaaca ttaaaacgaa agaacttatt catcgcatcg tgggcaacgg cggcagaaga
    13981 gcctaatccg aaaaaggcga ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat
    14041 ggcagattta tccagtgaat gacggacatg atcgagcgtt gccattgcgg actcaaatat
    14101 tttccccgtg tatgattcaa cgctgtcatc ttcattgacg ttgcgattaa catagggagt
    14161 gccattcgcc agactttgtg ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat
    14221 gctgcgacag aaacgattca ccgtcggttc gctaacattg gcttccagcg ccagagcagc
    14281 aatactcgaa tggatcgcgt tatcgggcga agccagaatg acctcggcaa ctttgcgctc
    14341 tgatttgctc aaatgttcca gctgagactg gattttttcc agcatattca tgatgtaaag
    14401 agactcacgg gtaatgacga tttccgcact gaaagaaatc gaaatgcagt tttgtcagat
    14461 attacgcctg tgtgccgtgt taatgacaaa agcagataaa aaagttgtta tttttttcat
    14521 aacatgatca gtgtcagatt tttaccgaat ggaaaacgat gattttttta tcagttttgc
    14581 cgcactttgc gcgcttttcc cgtaatcgca cgggtggata agcgtttaca gttttcgcaa
    14641 gctcgtaaaa gcagtacagt gcaccgtaag aaaattacaa gtataccctg gcttaagtac
    14701 cgggttagtt aacttaagga gaatgacatg gcggtaacgc aaacagccca ggcctgtgac
    14761 ctggtcattt tcggcgcgaa aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat
    14821 caactggaaa aagtcggtca gctcaacccg gacacccgga ttatcggcgt agggcgtgct
    14881 gactgggata aagcggcata taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa
    14941 gaaaccattg atgaaggttt atgggacacc ctgagtgcac gtctggattt ttgtaatctc
    15001 gatgtcaatg acactgctgc attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt
    15061 atcactatta actactttgc catgccgccc agcacttttg gcgcaatttg caaagggctt
    15121 ggcgaggcaa aactgaatgc taaaccggca cgcgtagtca tggagaaacc gctggggacg
    15181 tcgctggcga cctcgcagga aatcaatgat caggttggcg aatacttcga ggagtgccag
    15241 gtttaccgta tcgaccacta tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt
    15301 tttgctaact ccctgtttgt gaataactgg gacaatcgca ccattgatca tgttgagatt
    15361 accgtggcag aagaagtggg gatcgaaggg cgctggggct attttgataa agccggtcag
    15421 atgcgtgaca tgattcagaa ccacctgcta caaattctct gcatgattgc gatgtctccg
    15481 ccatctgacc tgagcgcaga cagcatccgc gatgaaaaag tgaaagtact gaagtccctg
    15541 cgccgcatcg accgctccaa cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc
    15601 ttcgcccagg gcaaaaaagt gccgggatat ctggaagaag agggcgcgaa caaaagcagc
    15661 aatacagaaa ccttcgtggc gatccgcgtc gacattgata actggcgctg ggccggtgtg
    15721 ccattctacc tgcgtactgg taaacgtctg ccgaccaaat gttctgaagt cgtggtctat
    15781 ttcaaaacac ctgaactgaa tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa
    15841 ctgactatcc gtctgcaacc tgatgaaggc gtggatatcc aggtactgaa taaagttcct
    15901 ggccttgacc acaaacataa cctgcaaatc accaagctgg atctgagcta ttcagaaacc
    15961 tttaatcaga cgcatctggc ggatgcctat gaacgtttgc tgctggaaac catgcgtggt
    16021 attcaggcac tgtttgtacg tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc
    16081 attactgagg cgtgggcgat ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg
    16141 ggacccgttg cctcggtggc gatgattacc cgtgatggtc gttcctggaa tgagtttgag
    16201 taatatcagc gcttatcctt tatggttatt ttaccggtaa catgatcttg cacagattgt
    16261 agaacaattt ttacactttc aggcctcgtg cggattcacc cacgaggctt tttttattac
    16321 actgactgaa acgtttttgc cctatgagct ccggttacag gcgtttcact cataaatcct
    16381 ctgaatgaaa cgcgttgtga atcatcctgc tctgacaact caatttcagg agcctttatg
    16441 aatccacaat tgttacgcgt aacaaatcga atcattgaac gttcgcgcga gactcgctct
    16501 gcttatctcg cccggataga acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca
    16561 tgcggtaacc tggcacacgg tttcgctgcc tgccagccag aagacaaagc ctctttgaaa
    16621 agcatgttgc gtaacaatat cgccatcatc acctcctata acgacatgct ctccgcgcac
    16681 cagccttatg aacactatcc agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt
    16741 ggtcaggttg cgggcggtgt tccggcgatg tgtgatggtg tcacccaggg gcaggatgga
    16801 atggaattgt cgctgctaag ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc
    16861 cataacatgt ttgatggtgc tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg
    16921 acgatggcag ccctgtcgtt tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg
    16981 gcaagcggtt tgccaaataa agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa
    17041 gtggaccgca tg
//
LOCUS       DADLCQ010000087        16905 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000087 DADLCQ010000000
VERSION     DADLCQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.087"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(173..1069)
                     /locus_tag="JQG95_002730"
     CDS             complement(173..1069)
                     /locus_tag="JQG95_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5889233.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            1174..1875
                     /locus_tag="JQG95_002731"
     CDS             1174..1875
                     /locus_tag="JQG95_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY5889234.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1898..2062
                     /locus_tag="JQG95_002732"
     CDS             1898..2062
                     /locus_tag="JQG95_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889235.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2274..3584)
                     /locus_tag="JQG95_002733"
     CDS             complement(2274..3584)
                     /locus_tag="JQG95_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY5889236.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3612..4943)
                     /locus_tag="JQG95_002734"
     CDS             complement(3612..4943)
                     /locus_tag="JQG95_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY5889237.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="JQG95_002735"
     CDS             complement(5218..6582)
                     /gene="tdcG"
                     /locus_tag="JQG95_002735"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY5889238.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6654..7043)
                     /locus_tag="JQG95_002736"
     CDS             complement(6654..7043)
                     /locus_tag="JQG95_002736"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY5889239.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="JQG95_002737"
     CDS             complement(7057..9351)
                     /gene="tdcE"
                     /locus_tag="JQG95_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY5889240.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="JQG95_002738"
     CDS             complement(9385..10593)
                     /gene="tdcD"
                     /locus_tag="JQG95_002738"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY5889241.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="JQG95_002739"
     CDS             complement(10619..11950)
                     /gene="tdcC"
                     /locus_tag="JQG95_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY5889242.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="JQG95_002740"
     CDS             complement(11972..12961)
                     /gene="tdcB"
                     /locus_tag="JQG95_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY5889243.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="JQG95_002741"
     CDS             complement(13060..13998)
                     /gene="tdcA"
                     /locus_tag="JQG95_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY5889244.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14188..14532
                     /gene="tdcR"
                     /locus_tag="JQG95_002742"
     CDS             14188..14532
                     /gene="tdcR"
                     /locus_tag="JQG95_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY5889245.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            14788..15327
                     /locus_tag="JQG95_002743"
     CDS             14788..15327
                     /locus_tag="JQG95_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889246.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            15349..15696
                     /locus_tag="JQG95_002744"
     CDS             15349..15696
                     /locus_tag="JQG95_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889247.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            15895..16354
                     /locus_tag="JQG95_002745"
                     /pseudo
     CDS             15895..16354
                     /locus_tag="JQG95_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005089572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4657 a         4313 c         3766 g         4169 t
ORIGIN      
        1 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc agcctacaaa
       61 ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag cgcatcaggc
      121 agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata gattattttc
      181 cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      241 gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc
      301 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      361 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      421 gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      481 atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      541 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      601 gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg
      661 cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
      721 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
      781 tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg gcttccagca
      841 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
      901 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
      961 cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg cgatcgatcg
     1021 catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat
     1081 tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt
     1141 accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag
     1201 tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac
     1261 ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga agtgctggcc
     1321 ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg
     1381 gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggagag
     1441 gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac
     1501 aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga gaatccgctg
     1561 attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc agagggtgcg
     1621 atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct cgacaaaggc
     1681 gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa
     1741 tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt
     1801 attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata
     1861 gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat cgaccaaaaa
     1921 gcgccagcca gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca aaaattttgg
     1981 ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa cgcgtttagc
     2041 cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg ccttatccgg
     2101 cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata agatgcgcca
     2161 gcatcgtatc cggcatcagg tacaaaacac aacaacatca acaagttgag caccccgtag
     2221 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2281 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2341 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2401 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2461 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2521 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2581 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2641 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2701 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttattgcagt gatcccctgg
     2761 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     2821 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     2881 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     2941 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaatgggact
     3001 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggcgac
     3061 tcttgctcgc cctctgccac acacgcctgg tgggtaaaca ccacaccatt gtgcgtctca
     3121 atatgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3181 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacagagact
     3241 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3301 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3361 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3421 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3481 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3541 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3601 tttcaggtaa atcaagaaaa tgccaggaac agagaaacac aaagcaacaa accagtgacg
     3661 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3721 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3781 aacaccggtg cgttcagtac gatggcgctc caggccagca aaatggcgaa aatcatgatg
     3841 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     3901 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     3961 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4021 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4081 tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4141 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat tgatttttcc
     4201 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacagaat cgacgtcagg
     4261 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4321 acgttataca gatgccacat tcccaccatc gacacgccca gcgcagcgac taccagcagc
     4381 ttggtcagca ccatgtcggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4441 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4501 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4561 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4621 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4681 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4741 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4801 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     4861 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     4921 cctttattcg atgcaatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     4981 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5041 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagacttta aaataacgga
     5101 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5161 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5221 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5281 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5341 ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc acggcattaa tggcattacg
     5401 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5461 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5521 taaccctgcc gctgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5581 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5641 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5701 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5761 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     5821 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac acggcacatt
     5881 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     5941 ttgccagata cgggcaaacc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6001 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6061 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6121 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6181 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6241 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6301 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6361 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6421 tgacaacgac ccgtacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6481 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6541 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6601 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6661 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6721 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6781 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     6841 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acgtgcctga
     6901 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     6961 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7021 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7081 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7141 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7201 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7261 atacccatcc agcaggccaa caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7321 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7381 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7441 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7501 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7561 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7621 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7681 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7741 cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac gatcgtgcag
     7801 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     7861 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     7921 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     7981 ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc gccaggttag cgcgtgcacc
     8041 aaagaactgc atttgcttac caatcaccat tgggctgacg cagcaggcaa tcgcgtaatc
     8101 gtcgctgttg aagtcagtac gcatcagatc gtcattttca tactgcaagg aagaggtgac
     8161 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8221 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8281 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8341 gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta cgcaggaagc gtaccatacg
     8401 gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc tgctcattga ggacgccagc
     8461 tttaaagtcg cgctcaatgt agatatccag gaacgatgcc gtgcgcccca gcgacatcgc
     8521 gccgccattt tgcgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8581 ctgtgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8641 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8701 atcctcgcct ttttccagac gagactggag atcggcaaat tgcagttcgc gttcacgcac
     8761 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacgacc
     8821 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     8881 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     8941 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9001 gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc aggccaacaa ttttttccag
     9061 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9121 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattacct tttcccacaa
     9181 ttccgtggtg gcaggcgtcg cttcggcgag aaaagattca tcgccttcat acggtgtata
     9241 gttatgttga ataaaatcgc ggacattaat ttcgcttttc cagtccgtac ctttaaagcc
     9301 aagccatgcg tcagcgtaca gcttatcgct ggtatcaata tctaccttca tgaaaaataa
     9361 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttacctaa
     9421 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacga cgcaaatgac
     9481 atgcgcattt tcactggaaa caattcgctc gccaaaagag ttagatcgat tattcatttc
     9541 tgtatcaatc tctacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9601 tgaattctct cctattccgc cggtgaatat aattccatcc agccgacgta atgaagctgc
     9661 atgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9721 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9781 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
     9841 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg tacccatcat
     9901 caagccttcc agcggcgtca ttcccattga ggtgtcaaca ctctgaccgt tgcgaactgc
     9961 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10021 attcagcagc gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10081 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10141 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10201 ttgctgcgcc gattcaatac cacttaaatt tgcgtaatta tgcaggggtg ccagtggaga
    10261 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10321 gccgccgtga gcgatgcggt ggccaattaa ggccacgctg tcatttaaat tccgtttttc
    10381 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10441 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10501 cattaatact tcacaatcgc tggcatcgag tacggaaaac ttaatcgaag acgaaccaca
    10561 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10621 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10681 tatccagacg accacggtat ttcgccagag acggcgcttt acggatagca tacatcggca
    10741 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10801 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgagagtat
    10861 tcagtttacc cagcgacact ttggttttgt cacctttata accgaacttc agaatcagac
    10921 cattcaagcc ttccagcgtc cccagatagt gaccgaagaa agatttgaag atagccacga
    10981 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttc gtaccggtca
    11041 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11101 ccatgttggc cggagacaga gtaaacaggc agctaaaggc aaagaacatc accactgcaa
    11161 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11221 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11281 agttaaagga gaaaaccatg atggaaatcc ccagccagac agtgatcagg ataccgtcat
    11341 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11401 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11461 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcaacagga
    11521 acagcgccac aaagccgcga ttcagcggtg caaatccgag ctggttttcc cagaacgtca
    11581 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11641 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11701 tgttgccgga agggttagag ccagaaagac acagacgcgc cagcgcccgg tggcaataaa
    11761 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    11821 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    11881 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    11941 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12001 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12061 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12121 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12181 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12241 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12301 ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac gccaataaca cgaatggtcg
    12361 ggttaataga tttaattgcc accgcaatac cagcaattaa accaccacca ccaattggca
    12421 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12481 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acgaccttcc atttcgacaa
    12541 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12601 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12661 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12721 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12781 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    12841 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    12901 gtcgttgttt cgcttcaata atatcatcaa tagcaaccgg cagatcgtat gtaatatgca
    12961 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13021 agacaggtgg attatttatg tttaaataaa aacaaacaac taaccaactt ctattaattg
    13081 ccttcgtcta cacccattat aagatgaata ctctttggct aattccacca aaaccgatgc
    13141 tgctttttta atacgataat ttttcgacca taccgcggca tattgtgcca caggtaatgt
    13201 ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa ggtgacgtca tatcacaagg
    13261 aattacagtt aagaaatcag cattgagaac aagattataa attgtcacga ctgagtcggt
    13321 tttaacgatg ttttcaatac tgatgccatt tctttgtaac gtagtaagca gttcgctgta
    13381 gtaccccata ttagtttgtg gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt
    13441 ggtgccggtg catgttcggg acttactggc taccagcaca aactcggact cgaacagcgg
    13501 ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac gtaccaatcg caaaatccag
    13561 tcgaccgtcg cggattgccg gtaagaacga agacagttgc gcttcataca tagaaacctg
    13621 cgctttcggg aacacctctt tgaacttgtt gatcatccct gacataaaag taaaaccaat
    13681 caatgaagga aaaccaaatg agacttccac caccgcttca gaagacatac cgcttatctc
    13741 attaaccata tttttcattt cacgggtaat ggattcggaa cgggagagta acaattgacc
    13801 ggcaggtgtt aatgttacac cggtattttt ccgcaccact aattccacac caaaataatc
    13861 ttcaatatcg ttaatgattt tactgacggc cggttgagtt aaccctaatt cttttgcagc
    13921 cgagccgata gagccacttc taatgacttc ctgaaagact accaggtgct gcgttttcgg
    13981 aagaagaata gtgctcataa cgacctacgt taattacctc attgacggca tgaagtgtat
    14041 caaaatgaaa taaacaggat atgtgcgacc actcacaaat taactttcat tactttccaa
    14101 ggtatcgtta ttacaattaa atatctttaa atatcaacaa gttaaagcat aaaaatcagc
    14161 ataaaccctg atttttatca aaaaaaaatg acggggataa ccatatttta tggcgataac
    14221 atcattcgtt atgtggttaa tacaaaaaag gggctgagac catatttcaa gcaattaccg
    14281 gataattacc aggcaaaatt tgagttaaat ttaatgagca agttctcaaa ttttattata
    14341 aataaaccat tttcagcgat aaatactgcg gcacgtcaca ttttttcacg ctatttgttg
    14401 gagaacaaac atttatttta tcaatatttt aaaatttcga atacaggtat tgatcatctc
    14461 gaacaattga ttaacgtcaa ctttttctct tctgacagga cgtcattttg tgaatgcaat
    14521 cgttttccat aaattcttct cccctcatag gcgacgaata gcattttgtg ttgaggatca
    14581 caaaacgaat aattgctggt cgccgcgata aggtcagacg aagtcaacaa gggggatttt
    14641 cactgtgctt tacatcagcg taggacacag aatgttgctt cgtttactgt aacgccgggt
    14701 aaatgagcgt tttttgatag tgcgtaagtg tcgtgcgagg aaaataagca tctattattg
    14761 tttgtcatga cgacaaggca ggcatcaatg aaaggcttcc caattgcaca tatttttcac
    14821 ccttcaatcc cgccaatgca cgcagtggtt aacaatcaca atagaaatat tgattattgg
    14881 acggtaaaaa gaaagtttgc agaaattgtc tccaccaatg acgttaataa aatttacagt
    14941 ataagtaatg aactccggag agtattatct gcaattactg cattgaattt ctatcaaggc
    15001 gatgttcctt ctgtcatgat ccgaatccaa ccggaaaata tgagtccatt catcatagat
    15061 atttctacag gagaacatga tgattatatc atacaaacat tagatgtagg cacttttgca
    15121 ccttttggtg aacaatgtac ttgctcagcc gtcaataaaa aagagctgga atgtattaag
    15181 gagacgattt ctaagtattg cgcaaagttc acccaaaaag aagccatctt aacaccccct
    15241 aggcacttta ataaaacctg cattacttca gattgttggc aaattctctt tttttcaccc
    15301 gatcatttta ataatgattt ttattgatta tttataaagg ataaaataat gttccctgtt
    15361 tcatcaattg gtaatgatat tagtagtgat ttagtccgtc gaaaaatgaa tgaccttcct
    15421 gaaagcccca taggaaataa cctcgaagca ctagcaccag gtatagaaaa actaaaacag
    15481 acctctattc aaatggtcac tttacttaac gcgttacaac caagtggaaa atgcattatc
    15541 actggtgatt ttcaaaaaga attagcgtac ttacaaaatg taattcttta taatgactcg
    15601 tctcttcgtc tggatttttt aggttataac acccaaatta ttcaacgatc ggacaatacc
    15661 tgcgaactta ccattaatga accgttaaaa aactaggaaa tatctacagg caatatcaat
    15721 gttaattgcc cattaaaaga tatttacaat gaaatcagga ggttgaacgt aatctttagt
    15781 tgtgggactg gagatatagt tgatctatcc tctctggact tacgtaatgt cgatttagat
    15841 tattatgatt tcacagataa acatatggct aatactattc taaatcattt taaattggat
    15901 tcaacaaatt ttactaatgc caacatgttt caggttaatt ttgttagttc aacacaaaac
    15961 gccacaatct cctgggatta tttactaaaa ataacgcctg ttttaataag cattagcgat
    16021 atgtattctg aagaaaaaat caagtttgtc gaaagttgtt taaatgagct tggagacatt
    16081 accgaagaac aattaaaaat tatgagattt gcaattataa aatctatacc aagggcaact
    16141 cttacagata aattagaaaa tgaattaaca aaagaaatat ataaaagctc atcgaaaatc
    16201 aacaattgct tgaacagaat taaattacca gagatgaaag aattctcatc aaaaaaaata
    16261 tatgattaca tcgatacaat cattgaagat tatgaaaata tcaaagaaaa tgcttatctg
    16321 gtcgtccccc aaattaatta tactatggat ttaaacatag aagactctag ctcagaagag
    16381 ttaataccct cgagaaagac gaaaattctc cggacaatgg ctttgaggtc ggggaatata
    16441 acacatatga agcatataac tcagagaatc aatattttac cagagaggac tatacgtata
    16501 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct attattaata
    16561 ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa
    16621 actgatggca accgcaaaaa tgcctgatgc gctacgctta tcaggcctac gccatctctg
    16681 caatatattg aatttgcgtg cttttgtagg caggataagg cgttcacgca gcatccggca
    16741 tgaataaagc gcactcaaca atctgaaacc cgccggaacg gtttattacg tacatcaggt
    16801 gaaactgacc gataagccgc tttcttttgg gtatagtgtc gtggacagtc attcatcttt
    16861 ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt acgta
//
LOCUS       DADLCQ010000088        16810 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000088 DADLCQ010000000
VERSION     DADLCQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.088"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..316
                     /locus_tag="JQG95_002746"
     CDS             <1..316
                     /locus_tag="JQG95_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5889248.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="JQG95_002747"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="JQG95_002747"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY5889249.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="JQG95_002748"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="JQG95_002748"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY5889250.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /locus_tag="JQG95_002749"
     CDS             complement(2160..3062)
                     /locus_tag="JQG95_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY5889251.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="JQG95_002750"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="JQG95_002750"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY5889252.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="JQG95_002751"
     CDS             complement(3720..4949)
                     /locus_tag="JQG95_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY5889253.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="JQG95_002752"
     CDS             complement(5013..5453)
                     /locus_tag="JQG95_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY5889254.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="JQG95_002753"
     CDS             complement(5558..6328)
                     /locus_tag="JQG95_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5889255.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="JQG95_002754"
     CDS             complement(6325..7251)
                     /locus_tag="JQG95_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5889256.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STAIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="JQG95_002755"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="JQG95_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY5889257.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="JQG95_002756"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="JQG95_002756"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY5889258.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /locus_tag="JQG95_002757"
     CDS             8805..11195
                     /locus_tag="JQG95_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY5889259.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="JQG95_002758"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="JQG95_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY5889260.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="JQG95_002759"
     CDS             12958..13305
                     /locus_tag="JQG95_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY5889261.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="JQG95_002760"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="JQG95_002760"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY5889262.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="JQG95_002761"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="JQG95_002761"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY5889263.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="JQG95_002762"
     CDS             complement(15125..15487)
                     /locus_tag="JQG95_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY5889264.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..>16810)
                     /locus_tag="JQG95_002763"
     CDS             complement(15662..>16810)
                     /locus_tag="JQG95_002763"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HAY5889265.1"
                     /translation="NRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLD
                     MPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLP
                     DLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNIVLLKWMIAEGYGDRRTLER
                     RIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPILCAPNDPDDARPLSAVQGEK
                     IDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGK
                     SGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALI
                     GKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKADGVIFQTAV"
BASE COUNT         4187 a         4537 c         4069 g         4017 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac caaggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgaacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agcggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt
//
LOCUS       DADLCQ010000089        16498 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000089 DADLCQ010000000
VERSION     DADLCQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.089"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            353..1711
                     /locus_tag="JQG95_002764"
     CDS             353..1711
                     /locus_tag="JQG95_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAY5889266.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="JQG95_002765"
     CDS             complement(1950..3335)
                     /locus_tag="JQG95_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5889267.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JQG95_002766"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="JQG95_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5889268.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="JQG95_002767"
     CDS             complement(3729..4109)
                     /locus_tag="JQG95_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY5889269.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="JQG95_002768"
     CDS             complement(4464..4898)
                     /locus_tag="JQG95_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889270.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="JQG95_002769"
     CDS             complement(4886..5287)
                     /locus_tag="JQG95_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889271.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="JQG95_002770"
     CDS             complement(5547..6116)
                     /locus_tag="JQG95_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY5889272.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7037)
                     /locus_tag="JQG95_002771"
     CDS             complement(6861..7037)
                     /locus_tag="JQG95_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAY5889273.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTMNK
                     LYDRAPASVWKYVT"
     gene            complement(7338..7604)
                     /locus_tag="JQG95_002772"
     CDS             complement(7338..7604)
                     /locus_tag="JQG95_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889274.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="JQG95_002773"
     CDS             7745..7951
                     /locus_tag="JQG95_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY5889275.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="JQG95_002774"
     CDS             8046..8648
                     /locus_tag="JQG95_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889276.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWGSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..10765
                     /locus_tag="JQG95_002775"
     CDS             9848..10765
                     /locus_tag="JQG95_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889277.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISSMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            10850..11722
                     /locus_tag="JQG95_002776"
     CDS             10850..11722
                     /locus_tag="JQG95_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY5889278.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            11940..14324
                     /locus_tag="JQG95_002777"
     CDS             11940..14324
                     /locus_tag="JQG95_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY5889279.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            14321..15226
                     /locus_tag="JQG95_002778"
     CDS             14321..15226
                     /locus_tag="JQG95_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY5889280.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            15223..16293
                     /locus_tag="JQG95_002779"
     CDS             15223..16293
                     /locus_tag="JQG95_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY5889281.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSETVL
                     QKSLEITEEADLALRSAMARIK"
BASE COUNT         4023 a         4244 c         4325 g         3906 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggggt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctag tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
    10621 agataaagtc aatgtacagt tttttcttga cgcagaaacc agggcgcagc tcaaagaatt
    10681 atcgagggtc agaagactca gtaccggcga aatgctgcat gacctgattg ttgaagagta
    10741 taaaaggtac cgacattcca gataatttca gacatatatt atctccgtga gtcccccgcc
    10801 acctttccgg tgtgcggggg ttttgtcttt tttcaccggg aatacatgta tgaatccgtc
    10861 tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactcac tttcccgtca
    10921 catcctggaa catctgcgca aactcaccag tcacgaaccc gtgattggca ttatgggtaa
    10981 aagcggggcc ggtaaatcct cactctgtaa tgcactgttt cagggggagg tcaccccggt
    11041 cagtgatgtt cacgccggca cccgggaagt gcagcgtttc cgtctgagtg gccatggtca
    11101 cagcatggtt atcactgacc tgcccggggt gggcgagagc cgggacagag atgcagagta
    11161 tgaagccctg taccgtgaca ttctgcctga acttgacctg gtactgtggc tgattaaagc
    11221 cgatgaccgt gccctgtctg tggatgagta tttctggcga cacatcctgc accggggaca
    11281 tcagcaggtg ctgtttgtgg tgacgcaggc cgacaaaacg gagccctgcc atgaatggga
    11341 tatggccggt attcagcctt ctcccgcaca ggcacagaat attcgcgaaa aaacggaggc
    11401 ggtattccgt ctgttccggc ccgtacatcc ggttgttgcc gtatcggccc gcaccggctg
    11461 ggaactggat acgctggtca gtgcgctaat gacagcactt cccgaccatg ccgccagtcc
    11521 cctgatgacc cgcctgcagg acgggctgcg cacggaatct gtccggggtc aggcccgtga
    11581 acagtttacc ggtgcggtgg accggatatt tgacacggcg gagagcgtct gtgttgcctc
    11641 tgttgcccgt aaggttctgc gcgctgttcg tgacaccgtg gtctctgttg cccgcgcggt
    11701 atggaactgg attttcttct gaacccggtg gtgatgcacc agactcttgt tacagatttt
    11761 taaccccatg aaaacacaat gcaaacacag ccatacccgg gttgtcccct tgcgttgtgt
    11821 ttctcaagtt aatagaatgc atcgatcgtt actatcatat cgatcgctat aaacgatcgt
    11881 ttgtggtaaa tgacctcctc cgtggttgtc atgaaaatgc gtctcaggaa ggttaaataa
    11941 tgcacgaaaa gaacatcgcc ctgctttgtg atgaagccga ccgacttttg caactgaaca
    12001 ttaatctgct ccggcaaatg gttgatgaac cggatgtgtt atctgacagt aagaacgaaa
    12061 acagactgct ttttgataaa cagaaagcac tgaaaagaat tgaggagctg gagggcgaac
    12121 aaatcaaaac cgcccgcagg gagatggtgc tggctgttgt cggcacgatg aaagcaggta
    12181 aatcaactac catcaacgcc attgtggggc aggaaattct gcctaaccgt aaccgcccca
    12241 tgacctctgt accgacgctc atccgccacg ttcccggaaa aactgagccg gtcctccatc
    12301 tggaacatat tcagcccgtc cgcaatttat taagcacact gcaacaaaaa ctcgacaccc
    12361 cggcaggaca gcaagtcgca cagaccctgc agcaaaccgg ggatacctgc gaactgctgg
    12421 atattctggc aaatgatggc tggctcaaaa atgaatacca cggggaagac gaaatcttta
    12481 ccggactggc atcgttaaac gatctggttc gccttgctgc ggcaatgggg agtgagtttc
    12541 cttttgatga atacgcagaa gtgcagaaac tgccggtgat cgacgtggaa ttcagccatc
    12601 tggtggggat ggatgcatgc cagggaacac tcacactgct ggataccccc ggccctaatg
    12661 aggccggaca accgcagatg gaagtgatga tgcgggatca actgcagaaa gcctctgcgg
    12721 tgctggcggt gatggattac acccagatga actcaaaagc ggatgaagac gtccgtaaag
    12781 agcttaatgc cattgctgac gtatcagccg gccgcctgtt tgtgctggtc aataaatttg
    12841 atgagaaaga ccgcaatggc gatggggcag atgccgtacg ccagaaagtt ccggcaatgc
    12901 tgaacagcga tgtgctgccc gcctcccgcg tttatccagg ctcatcacgt caggcgtacc
    12961 tggctaaccg tgcgcttcat gagttacgga aaaacagaac ccttcctgtt gatgaagcct
    13021 gggtcgatga ttttgtcagg gaagccttcg gtcgcatgaa aaaagaatac gtctgtaaag
    13081 acagtgaaat ggcaaccgaa ggggcgacag acctgtggga aggctcactt atcgatcaac
    13141 tgataacgga agtcatacag agctcccatt ccagagcggc ggcactggcg gttgattctg
    13201 ccgccgcaaa actgatgcag aatgcggaaa atgtcagcga atatctgtca ttacgccatc
    13261 aggggctaca gcaaagcatt cagtcactgc agtcgcatat caccagcctg cttgcggata
    13321 tccgggaaat cgcggactgt caggagcagg tgaccgctga tgtcagaatg gccatggagg
    13381 agatcgatgc cagaacccgg gaattactga cgggggtctg cacctcactg gaagaggagc
    13441 tgaatgacta tttcagaagc ggtaaacgca aagaacagca aatgctggag gaagaaaatt
    13501 cagcgcagcc ccgggagaga aacgcattcg ctttctttca cgatattttc ggaaccggaa
    13561 atcagcatga caggatgcgg gactttgatc cggacagccc cgaaataaaa ttcagtgatc
    13621 gcagggaagc ccttgaactg atgacgcaaa tcgaatcgac cgtgaccagc ctgcaccgtg
    13681 aggctgaagc acagttccgt cctgaactgg aaaaaatcgt caggggtatt gaaacaggtt
    13741 ttcgtggtac ggccctgtac gccacagaaa atattgccgg acgcatcaat gcccgtctgg
    13801 aggatgaagg tttcactgta aaaatcagtt ttccggcagt cagccagtta cagacccggc
    13861 tcgcagtaaa aacaaatctg agcgcgctta tggaggaaag aacggagaca gtaacccgtc
    13921 gctgtcgaca ggatggtgtc tggggtacgc tttgtcgatg ggcaaacacc agtgactggg
    13981 gctggaaaga gtacagtgtg gatgtgagcc gcagcgtgat caatatcaac aaggtcagag
    14041 aggaagtgat gtcactgacc cgggcatatt tcggggagct gcaggcatcc attgagcagg
    14101 atattaacca gcccgtccgc caggaaatcg atgccttttt ctgtacattc agggagaaag
    14161 ttgaacagct gcgtaacacg ctgattcaga gctctgaaga tcataaacgc gatcagcagg
    14221 cgcaggaacg gctgaccggg cgtcttcagg cgttaaatga aagggttcct gagctcatta
    14281 ctgacagtaa ggcgttaagg gaagaactgg agacaatgct gtgacttcac catttattca
    14341 acaaattgct gataaccggg tatgtcaggt actcacctgt cttcctgaaa aatttgtggt
    14401 tgatttcgca aacggtattg atgttgcaca ggagcatatc cgtacggccg gagagcgcac
    14461 gttcttccgg cggttaaaag aggggctgag cggcgaaggt actgcacggc agaatgccat
    14521 caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc tggctgacgg agctgacaac
    14581 gtcacttgcc accacaaact acgcgattac ccgggtaaac gacagggtca gttcactggt
    14641 cagtgatact gccaggctgg cgcattattc cgcagatacg cgggagcagt tactcaccct
    14701 ggccgatcag gttcaccaga aactgaatca tcttgaagaa aaactccacc gtgttgacct
    14761 ggttcagcgg gcgcagttac atcttgagca gatattctcc tggtggagcg ccgggcgata
    14821 tgcgtcattt tcccctgccg gacgttgtta tgtggcactt gaagagcttc gctggggcgc
    14881 gtttggtgat gtgatacgtc agggcgaaac aggccaggtt aaccagctac tggatattct
    14941 cagacataaa gcattaacgc agatggcaca ggagagtggc ggtagtgcaa ccgtgcgtct
    15001 gaacactctg gactggcttg gtggtcaggg ccgggagcag gcagataacg agtggcatga
    15061 cgcgattaac tggctggggg actggtgcag tgaagagcag catccggtga tctggtcaac
    15121 cacgcaggct gcagaacact taccggttcg catgccccgt ctctgctctg cagaacgcct
    15181 ctctgaaagt atggtcgatg aaatatttca gaagggggct gcatgagtat agaaataaaa
    15241 acagggctgg tgctgtccgg cggtggggcg gtgggcgctt atcaggcggg agtggttaag
    15301 gcactggcag agtgtggtac acagatcagc atggtttcag gggccagcat tggcgcattc
    15361 aatggtgcca ttatcgcggc ctctcccgat ctgtcagaag ctgccgtacg cctggaggcg
    15421 ctctgggatc atcttgggaa taatcaggtg ctgtcggtaa accgatcggt ttacttttca
    15481 ttgctgaaaa aattgttcca ggcaatgaac ctctgccaga tccccggacg tgcaggagca
    15541 ctgcttacga cacttcttcg ccatatatcg ataatcaacg gcttcgacaa tccgatgact
    15601 cagccgttgt tgtcagatgg gcccctgaca gcgttgatgg atcattatct tgatacagat
    15661 gctctggcag gcgggctacc gctgtatgtg tcgctgtacc ccacagaagg gggcatgcag
    15721 gatattattg actgcattcg tgctgaactg ggttccggaa ccacgaaaaa cgccgttttt
    15781 cagcatatcc agagtctgcc ccgcggacag cagaaagagg ctctgcttgc gtcagccgca
    15841 ctgcccctgt tgttccgtcc ccgtgaggtt caggggacaa tgttcggtga tggtggtatg
    15901 ggaggatggc gaaatatgca gggaaatacc cctgtaacgc ctctggtcga tgccggatgc
    15961 aatatggtga ttgtgtcgca tctgagtgac ggttctttat gggatcgcca ggcttttccg
    16021 gacaccacaa tccttgagat ccgtccccgg aaaaggctga aatatgcagg tgatggtggc
    16081 aacagtggcg gtctgctcag tttcacgtcg gcacataccg acgcctggcg tcagcagggc
    16141 tacgaggaca cgatgctgac gatggagcat atccggaaac cgctggcagc acgtcaggca
    16201 ctgtcccggt cagaaacggt gttgcagaaa agtctggaga taacggaaga ggcagatttg
    16261 gcactgagaa gcgcgatggc ccggattaaa taaagacgcc ccaaagaaac gccaccgcct
    16321 gtggtggtgt ttttcctgtc tggtgttgtt gaaaatattt tcagatatat attacctggg
    16381 tgagatttaa gattaacgca tttattaatg tcataaggag agttatcatg aaattcatcg
    16441 taaaagcact taggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttga
//
LOCUS       DADLCQ010000090        16387 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000090 DADLCQ010000000
VERSION     DADLCQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.090"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="JQG95_002780"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="JQG95_002780"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY5889282.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JQG95_002781"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JQG95_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY5889283.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="JQG95_002782"
     CDS             2337..2552
                     /locus_tag="JQG95_002782"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889284.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="JQG95_002783"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="JQG95_002783"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY5889285.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /locus_tag="JQG95_002784"
     CDS             4447..5841
                     /locus_tag="JQG95_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY5889286.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="JQG95_002785"
     CDS             5918..6415
                     /locus_tag="JQG95_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY5889287.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="JQG95_002786"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="JQG95_002786"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY5889288.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="JQG95_002787"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="JQG95_002787"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY5889289.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="JQG95_002788"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="JQG95_002788"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY5889290.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9028..9663
                     /locus_tag="JQG95_002789"
     CDS             9028..9663
                     /locus_tag="JQG95_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY5889291.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIAGRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="JQG95_002790"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="JQG95_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY5889292.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="JQG95_002791"
     CDS             complement(10255..11235)
                     /locus_tag="JQG95_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY5889293.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="JQG95_002792"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="JQG95_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY5889294.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="JQG95_002793"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="JQG95_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY5889295.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="JQG95_002794"
     CDS             12538..12828
                     /locus_tag="JQG95_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY5889296.1"
                     /translation="MSAVTVNDDGLVLQLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="JQG95_002795"
     CDS             12836..13429
                     /locus_tag="JQG95_002795"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY5889297.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="JQG95_002796"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="JQG95_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY5889298.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="JQG95_002797"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="JQG95_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16383
                     /locus_tag="JQG95_002798"
     CDS             15880..16383
                     /locus_tag="JQG95_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY5889299.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4058 a         4220 c         4126 g         3983 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttcta cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaagc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga gtcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggccgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggactgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaactga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 ccttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac tatattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 cgccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcacttgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccgg ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gtgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgagcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata caatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgccatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacagc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgtgcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ctatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aatcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtggc gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattaac
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgtgtt ttattaatca aagcattcaa caactgaaac aacctgcgca ggaggccagt
    16381 taatgga
//
LOCUS       DADLCQ010000091        16030 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000091 DADLCQ010000000
VERSION     DADLCQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.091"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            95..2248
                     /locus_tag="JQG95_002799"
     CDS             95..2248
                     /locus_tag="JQG95_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889300.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="JQG95_002800"
     CDS             2301..4031
                     /locus_tag="JQG95_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889301.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQG95_002801"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="JQG95_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY5889302.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="JQG95_002802"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="JQG95_002802"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY5889303.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="JQG95_002803"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="JQG95_002803"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY5889304.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQG95_002804"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="JQG95_002804"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY5889305.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="JQG95_002805"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="JQG95_002805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY5889306.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="JQG95_002806"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="JQG95_002806"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY5889307.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="JQG95_002807"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="JQG95_002807"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY5889308.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNCRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="JQG95_002808"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="JQG95_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY5889309.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQG95_002809"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="JQG95_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY5889310.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQG95_002810"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="JQG95_002810"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY5889311.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQG95_002811"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="JQG95_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY5889312.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQG95_002812"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="JQG95_002812"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY5889313.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQG95_002813"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="JQG95_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY5889314.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..>16030)
                     /gene="nrdR"
                     /locus_tag="JQG95_002814"
     CDS             complement(15689..>16030)
                     /gene="nrdR"
                     /locus_tag="JQG95_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY5889315.1"
                     /translation="RFTTFEVAELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVS
                     SDYVEMAINHIKSQLRATGEREVPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIK
                     EFGEEIARLED"
BASE COUNT         4366 a         3956 c         3861 g         3847 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatctacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttatga tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaattgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgc
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgtgcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt tcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg
//
LOCUS       DADLCQ010000092        15580 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000092 DADLCQ010000000
VERSION     DADLCQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.092"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            248..631
                     /gene="secE"
                     /locus_tag="JQG95_002815"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="JQG95_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY5889316.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPIRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="JQG95_002816"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="JQG95_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY5889317.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="JQG95_002817"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="JQG95_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY5889318.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="JQG95_002818"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="JQG95_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY5889319.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="JQG95_002819"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="JQG95_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY5889320.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="JQG95_002820"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="JQG95_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY5889321.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="JQG95_002821"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="JQG95_002821"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY5889322.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="JQG95_002822"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="JQG95_002822"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY5889323.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="JQG95_002823"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="JQG95_002823"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY5889324.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14680)
                     /gene="thiG"
                     /locus_tag="JQG95_002824"
     CDS             complement(13835..14680)
                     /gene="thiG"
                     /locus_tag="JQG95_002824"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY5889325.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHATQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEVGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="JQG95_002825"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="JQG95_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY5889326.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="JQG95_002826"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="JQG95_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY5889327.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
BASE COUNT         3779 a         3997 c         4232 g         3572 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgattcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacttg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt
//
LOCUS       DADLCQ010000093        15528 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000093 DADLCQ010000000
VERSION     DADLCQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.093"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="JQG95_002827"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="JQG95_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY5889328.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="JQG95_002828"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="JQG95_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY5889329.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="JQG95_002829"
     CDS             complement(941..1408)
                     /locus_tag="JQG95_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY5889330.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1789..2100
                     /gene="rpsJ"
                     /locus_tag="JQG95_002830"
     CDS             1789..2100
                     /gene="rpsJ"
                     /locus_tag="JQG95_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY5889331.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2133..2762
                     /gene="rplC"
                     /locus_tag="JQG95_002831"
     CDS             2133..2762
                     /gene="rplC"
                     /locus_tag="JQG95_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY5889332.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2773..3378
                     /gene="rplD"
                     /locus_tag="JQG95_002832"
     CDS             2773..3378
                     /gene="rplD"
                     /locus_tag="JQG95_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY5889333.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3375..3677
                     /gene="rplW"
                     /locus_tag="JQG95_002833"
     CDS             3375..3677
                     /gene="rplW"
                     /locus_tag="JQG95_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY5889334.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3695..4516
                     /gene="rplB"
                     /locus_tag="JQG95_002834"
     CDS             3695..4516
                     /gene="rplB"
                     /locus_tag="JQG95_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY5889335.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4533..4811
                     /gene="rpsS"
                     /locus_tag="JQG95_002835"
     CDS             4533..4811
                     /gene="rpsS"
                     /locus_tag="JQG95_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY5889336.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4826..5158
                     /gene="rplV"
                     /locus_tag="JQG95_002836"
     CDS             4826..5158
                     /gene="rplV"
                     /locus_tag="JQG95_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY5889337.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5176..5877
                     /gene="rpsC"
                     /locus_tag="JQG95_002837"
     CDS             5176..5877
                     /gene="rpsC"
                     /locus_tag="JQG95_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY5889338.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5890..6300
                     /gene="rplP"
                     /locus_tag="JQG95_002838"
     CDS             5890..6300
                     /gene="rplP"
                     /locus_tag="JQG95_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY5889339.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6300..6491
                     /gene="rpmC"
                     /locus_tag="JQG95_002839"
     CDS             6300..6491
                     /gene="rpmC"
                     /locus_tag="JQG95_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY5889340.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6491..6745
                     /gene="rpsQ"
                     /locus_tag="JQG95_002840"
     CDS             6491..6745
                     /gene="rpsQ"
                     /locus_tag="JQG95_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY5889341.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6910..7281
                     /gene="rplN"
                     /locus_tag="JQG95_002841"
     CDS             6910..7281
                     /gene="rplN"
                     /locus_tag="JQG95_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY5889342.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7292..7606
                     /gene="rplX"
                     /locus_tag="JQG95_002842"
     CDS             7292..7606
                     /gene="rplX"
                     /locus_tag="JQG95_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY5889343.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7621..8160
                     /gene="rplE"
                     /locus_tag="JQG95_002843"
     CDS             7621..8160
                     /gene="rplE"
                     /locus_tag="JQG95_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY5889344.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8175..8480
                     /gene="rpsN"
                     /locus_tag="JQG95_002844"
     CDS             8175..8480
                     /gene="rpsN"
                     /locus_tag="JQG95_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY5889345.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8514..8906
                     /gene="rpsH"
                     /locus_tag="JQG95_002845"
     CDS             8514..8906
                     /gene="rpsH"
                     /locus_tag="JQG95_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY5889346.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8919..9452
                     /gene="rplF"
                     /locus_tag="JQG95_002846"
     CDS             8919..9452
                     /gene="rplF"
                     /locus_tag="JQG95_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY5889347.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9462..9815
                     /gene="rplR"
                     /locus_tag="JQG95_002847"
     CDS             9462..9815
                     /gene="rplR"
                     /locus_tag="JQG95_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY5889348.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9830..10333
                     /gene="rpsE"
                     /locus_tag="JQG95_002848"
     CDS             9830..10333
                     /gene="rpsE"
                     /locus_tag="JQG95_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY5889349.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10337..10516
                     /gene="rpmD"
                     /locus_tag="JQG95_002849"
     CDS             10337..10516
                     /gene="rpmD"
                     /locus_tag="JQG95_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY5889350.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10520..10954
                     /gene="rplO"
                     /locus_tag="JQG95_002850"
     CDS             10520..10954
                     /gene="rplO"
                     /locus_tag="JQG95_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY5889351.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10962..12293
                     /gene="secY"
                     /locus_tag="JQG95_002851"
     CDS             10962..12293
                     /gene="secY"
                     /locus_tag="JQG95_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY5889352.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12325..12441
                     /gene="rpmJ"
                     /locus_tag="JQG95_002852"
     CDS             12325..12441
                     /gene="rpmJ"
                     /locus_tag="JQG95_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY5889353.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12588..12944
                     /gene="rpsM"
                     /locus_tag="JQG95_002853"
     CDS             12588..12944
                     /gene="rpsM"
                     /locus_tag="JQG95_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY5889354.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12961..13350
                     /gene="rpsK"
                     /locus_tag="JQG95_002854"
     CDS             12961..13350
                     /gene="rpsK"
                     /locus_tag="JQG95_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY5889355.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13384..14004
                     /gene="rpsD"
                     /locus_tag="JQG95_002855"
     CDS             13384..14004
                     /gene="rpsD"
                     /locus_tag="JQG95_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY5889356.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14030..15019
                     /gene="rpoA"
                     /locus_tag="JQG95_002856"
     CDS             14030..15019
                     /gene="rpoA"
                     /locus_tag="JQG95_002856"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY5889357.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15060..15443
                     /gene="rplQ"
                     /locus_tag="JQG95_002857"
     CDS             15060..15443
                     /gene="rplQ"
                     /locus_tag="JQG95_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY5889358.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4008 a         3645 c         4130 g         3745 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt actattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccaatcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agagcgctga tttgttaacc tgattccggt
     1501 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1561 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1621 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1681 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1741 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1801 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     1861 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     1921 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     1981 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2041 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2101 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2161 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2221 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2281 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2341 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2401 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2461 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2521 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2581 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2641 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2701 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2761 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     2821 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     2881 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     2941 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3001 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3061 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3121 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3181 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3241 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3301 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3361 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3421 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcga
     3481 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3541 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3601 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3661 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3721 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3781 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     3841 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     3901 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     3961 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4021 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4081 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4141 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4201 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4261 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4321 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4381 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4441 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4501 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4561 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4621 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4681 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4741 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4801 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     4861 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     4921 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     4981 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5041 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5101 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5161 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5221 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5281 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5341 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5401 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5461 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5521 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5581 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5641 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5701 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5761 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     5821 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     5881 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     5941 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6001 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6061 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6121 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6181 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6241 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6301 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6361 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6421 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6481 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6541 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt
     6601 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6661 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6721 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6781 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     6841 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     6901 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     6961 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7021 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7081 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7141 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7201 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7261 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7321 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7381 ttccggcaag gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7441 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7501 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7561 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7621 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7681 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7741 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7801 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     7861 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     7921 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     7981 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8041 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8101 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8161 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8221 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8281 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8341 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8401 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8461 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8521 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8581 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8641 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8701 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8761 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     8821 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     8881 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     8941 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9001 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9061 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9121 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9181 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9241 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9301 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9361 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg tgttcgttac gccgacgaag
     9421 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9481 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9541 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9601 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9661 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9721 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9781 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
     9841 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
     9901 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
     9961 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10021 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10081 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10141 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10201 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10261 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10321 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10381 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10441 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10501 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10561 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10621 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10681 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10741 agccgaagtt cgtctatctg acctggctaa agtagaaggc ggtgttgtag acctgaacac
    10801 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    10861 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    10921 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    10981 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11041 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11101 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11161 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11221 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11281 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11341 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11401 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11461 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11521 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11581 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11641 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11701 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11761 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    11821 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    11881 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    11941 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12001 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12061 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12121 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12181 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12241 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12301 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12361 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12421 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12481 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12541 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12601 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12661 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12721 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12781 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    12841 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    12901 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    12961 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13021 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13081 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13141 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13201 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13261 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13321 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13381 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13441 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13501 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13561 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13621 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13681 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13741 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13801 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    13861 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    13921 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    13981 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14041 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14101 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14161 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14221 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14281 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14341 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt
    14401 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14461 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14521 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14581 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14641 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14701 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14761 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    14821 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    14881 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    14941 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15001 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15061 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15121 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15181 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg
    15241 ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta
    15301 acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg
    15361 gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga
    15421 aagcagaagc tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt
    15481 ttttataccc gtagtatccc cacttatcta caatagctgt actctttt
//
LOCUS       DADLCQ010000094        15464 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000094 DADLCQ010000000
VERSION     DADLCQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.094"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..74)
                     /locus_tag="JQG95_002858"
     CDS             complement(<1..74)
                     /locus_tag="JQG95_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HAY5889359.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="JQG95_002859"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="JQG95_002859"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY5889360.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="JQG95_002860"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="JQG95_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY5889361.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="JQG95_002861"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="JQG95_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY5889362.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="JQG95_002862"
     CDS             2704..2940
                     /locus_tag="JQG95_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY5889363.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="JQG95_002863"
     CDS             2943..3800
                     /locus_tag="JQG95_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5889364.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5797
                     /locus_tag="JQG95_002864"
                     /pseudo
     CDS             3800..5797
                     /locus_tag="JQG95_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="JQG95_002865"
     CDS             complement(5798..6319)
                     /gene="sodC"
                     /locus_tag="JQG95_002865"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY5889365.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6400..7296)
                     /locus_tag="JQG95_002866"
     CDS             complement(6400..7296)
                     /locus_tag="JQG95_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY5889366.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7345..7584)
                     /locus_tag="JQG95_002867"
     CDS             complement(7345..7584)
                     /locus_tag="JQG95_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY5889367.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7687..8286
                     /locus_tag="JQG95_002868"
     CDS             7687..8286
                     /locus_tag="JQG95_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY5889368.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8323..9420
                     /gene="nemA"
                     /locus_tag="JQG95_002869"
     CDS             8323..9420
                     /gene="nemA"
                     /locus_tag="JQG95_002869"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY5889369.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9501..9908
                     /gene="gloA"
                     /locus_tag="JQG95_002870"
     CDS             9501..9908
                     /gene="gloA"
                     /locus_tag="JQG95_002870"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY5889370.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10011..10658
                     /gene="rnt"
                     /locus_tag="JQG95_002871"
     CDS             10011..10658
                     /gene="rnt"
                     /locus_tag="JQG95_002871"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY5889371.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10751..15209
                     /locus_tag="JQG95_002872"
                     /pseudo
     CDS             10751..15209
                     /locus_tag="JQG95_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15253..>15464)
                     /locus_tag="JQG95_002873"
     CDS             complement(15253..>15464)
                     /locus_tag="JQG95_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5889372.1"
                     /translation="CACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAV
                     LACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3546 a         4092 c         4064 g         3762 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgcaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttacttttgg tcaaatttgg cctgatggtc cagattagcg atctgtggca
     5101 atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat tgcagatgcc
     5161 caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta tcgctgtcac
     5221 taatcccccg gtgtatgatt ttgctgattt tcttaactat aatctggcaa aaatcgttgg
     5281 cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg ctcgtaaaag
     5341 ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc gccatccaac
     5401 actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt tgagtaacag
     5461 ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc tgaactgttc
     5521 tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat cgcgagtacg
     5581 ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc agcaaaaatc
     5641 actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc gtcatcatca
     5701 acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc tttcgcaact
     5761 tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca ccacaggcat
     5821 agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg ccgccaacgt
     5881 ggaccatcag cgccttgtct ttgatttcat ccagtgattt cagacgaggc gcgatgacgg
     5941 catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct aaatgcccgg
     6001 taccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg cctgcggatt
     6061 ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg gcatgaatat
     6121 ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg tgaaaactcc agacctttat
     6181 cggtttcagt aatggtgacg ctaccaattg actgcccaac cccttgcgac gtgacgaggt
     6241 tcatctcgac tttttcactg gcagcttgtg cgccggtcgc aacaaccagc gccagaatgg
     6301 ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata agtgtaaacc
     6361 agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt cgtaccccag
     6421 tgcagcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt ctgcttcgac
     6481 agctgcccgt acgcgctcaa ttttacctga gccgataatt ggcagcggct gcgacggtaa
     6541 acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact cctccgccac
     6601 cacagccagt tcatcacgca gcggctggaa ataatcatca ttaaacagac gaccaccacc
     6661 aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga gcgtgccatc
     6721 cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa gggtaaacgg
     6781 cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc caaaatgacg
     6841 cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg catccattaa
     6901 cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat taattagcga
     6961 ctgttcggcg ctcttaatga tgtgattgcg gtcagtgatg taatgaccaa tgacgttttc
     7021 ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt cacgcaggtg
     7081 aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac cataaatatc
     7141 agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac tgaccagctg
     7201 gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac gggaaaactc
     7261 cgggccttcc ggcgcaatag taatacgctg aaccataatc gcttcctctt atcagatata
     7321 agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt cgtctgatga
     7381 cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc gcagcacctc
     7441 ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac tacgaaaaca
     7501 gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc tctggacggg
     7561 aaagaactct aattgctccg ccacctcgcc ctcctcagat aagattatta ccattattga
     7621 agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc tactacactc
     7681 caacgcatga acaaacacac cgaacatgat actcgcgaac atctcttggc gacgggcgag
     7741 caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact aaaaaccgct
     7801 gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt tggcgttgcc
     7861 atgcttgagc gtcattacgc cgcatatcac cagcgactgg ctgagttgct gcaatccggc
     7921 gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa ccagttttgc
     7981 caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt gtgcgatctg
     8041 tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcgc cctgctctcg
     8101 caggctctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga accgctacaa
     8161 caggcacaag tgctttacgc actatggctt ggcgcgaatc tgcaggccaa aatttcgcgc
     8221 agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc gacgcctgcc
     8281 gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc tgaaaaactg
     8341 tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat ggcaccgctg
     8401 acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc ggaatactat
     8461 cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cacaaatttc tgcccaggca
     8521 aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc atggaaaaaa
     8581 atcaccgctg gcgttcatgc tgaaaatggt catatggcct tgcagctgtg gcacaccgga
     8641 cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc ttcagcactt
     8701 agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg cgttgaaaca
     8761 tccatgccgc gtgcgcttga actggaagag attccgggta tcgtcaatga tttccgtcag
     8821 gccattgcta acgcgcgtga agccgctttt gatctggtag agctccactc tgctcacggt
     8881 tatttgctgc atcagttcct ttccccttct tcaaaccatc gtaccgatca gtacggcggc
     8941 agcgtggaaa atcgcgcacg tttggtactg gaagtggtcg atgccgggat tgaagaatgg
     9001 ggtgccgatc gcattggcat tcgcgtttcg ccaatcggta ctttccagaa cacagataac
     9061 ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa acgcggcatt
     9121 gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac tgatgcgttc
     9181 cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc atacacagta
     9241 gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt tggtcgtgac
     9301 tggattgcga acccggatct ggtcgcccgc ttgcagctca aagctgagct taacccacag
     9361 cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc gacgttgtaa
     9421 tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt gaatctgtta
     9481 gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt ggcgatttgc
     9541 aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt accagcgaaa
     9601 acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc gaagaagcgg
     9661 tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact gcttatggtc
     9721 acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt caaaacgggg
     9781 gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc gcgtttgtgg
     9841 aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt cgcggtctgg
     9901 gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg catcgacaag
     9961 taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag atgtccgata
    10021 acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt gtgatcgatg
    10081 ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc gccatcaccc
    10141 tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc cacgtcgaac
    10201 catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt gacccgaacg
    10261 atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt aaagttgtac
    10321 gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac aacgccaatt
    10381 ttgatcacag ctttatgatg gccgccgcag aacgcgcctc gctgaaacgt aacccgttcc
    10441 accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga caaaccgtat
    10501 tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg cactccgcgc
    10561 tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc tggaaacgtc
    10621 tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc ctgatgacat
    10681 gcaatgattc aggcatctat agtgaggcta ttccacgcat caagcatgat attcacgggg
    10741 aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg tgttttcacc
    10801 ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc agtcgcaaac
    10861 ctggcatgtg gcggcgcgag gcgaacatgc gctggtgatt gcaccgactg gctccgggaa
    10921 aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg gcgaagatac
    10981 ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga taaaagccct
    11041 gggcaccgac gttcagcgca acttgcagat cccgttgaag ggtattgccg atgaacggcg
    11101 gcggcgcggc gaaacggtaa ttattgatga agtccacgcg gtagcgggca gtaaacgtgg
    11161 tgcgcatctg gcgttaagtc tggagcggct cgatgcgctg ctccacacct cagcacagcg
    11221 aattggcctt tctgccactg tgcgctcagc cagcgatgtg gcagcatttc ttggtggcga
    11281 tcgcccggtt acggtagtca acccgcccgc aatgcgccat ccgcagatac gaattgtcgt
    11341 accggtcgcc aatatggatg atgtctcatc ggtcgccagc ggcaccggcg aagacagcca
    11401 tgccggccgg gaaggctcca tctggccata tattgaaacg ggtatccttg atgaagtgtt
    11461 gcgccatcgc tcgaccattg tctttactaa ttcacgcggg ctggcggaaa aactgacagc
    11521 acgattaaat gagctttacg ccgcacgctt acagcgttcc ccgtctatcg ccgttgatgc
    11581 ggcccatttc gagtcgacct ccggcgcaac ctctaaccgt gtacaaagta gcgacgtttt
    11641 tattgcccgc tcacaccacg gctccgtctc taaagaacaa cgagcaatca ccgaacaggc
    11701 gctgaaatcg ggtgaattac gctgcgtggt cgcaacctcc agtcttgaac tggggattga
    11761 tatgggcgcg gtggatctgg tgattcaggt ggcaacgccg ctttctgttg ccagtgggtt
    11821 acaacgcatt ggtcgcgccg gacatcaggt tggcggtgta tctaaagggc tgtttttccc
    11881 ccgtacccgg cgtgatttag tcgattccgc agtcattgta gagtgtatgt tcgcaggcag
    11941 gctggaaaac ctgacaccac cgcataatcc tctcgacgtc cttgcgcagc aaaccgttgc
    12001 cgccgcggcg atggatgcat tacaggtaga cgaatggtac tcccgcgtac gccgtaccgc
    12061 accgtggaaa gatctgccaa gacgtgtttt tgacgccacg ctggatatgc tttccgggcg
    12121 ctatccctct ggcgattttt ctgcttttcg ccccaaactg gtctggaaca gggagaccgg
    12181 gatattgacc gcccgacctg gcgctcaatt gttggcggtt accagcggcg gcaccattcc
    12241 ggatcgtggc atgtatagcg tgttattacc cgaaggtgaa gaaaaggccg gttcgcggcg
    12301 ggtgggtgaa ctggatgagg agatggtata tgagtcgcgg gtgaacgaca ttatcactct
    12361 cggcgctacc tcatggcgga tccagcaaat cacccgcgat caggtgattg tgactcctgc
    12421 tccgggtcgt tctgcccagc tccccttctg gcgtggtgaa ggtaacggac gtccggctga
    12481 attaggcgag atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg
    12541 gactattccc ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga
    12601 cgagcaacgc aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg
    12661 ccgtgatgaa attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca
    12721 tgaaccctgg gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc
    12781 tgtggtcgcc agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc
    12841 cgacgccgcg atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt
    12901 aggcagctcg gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat
    12961 gccgggccgc acgccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca
    13021 gttgctggaa atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga
    13081 atgtctgcaa gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg
    13141 tggcgaaatt caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt
    13201 attgttcggc tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg
    13261 ggcatccgta ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc
    13321 gggggaatta ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc
    13381 tcctggtaga agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc
    13441 aatgaccgtt gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta
    13501 tctggaaaat cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg
    13561 tcttgcctgt atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac ggctaccaga
    13621 gtcattgcca gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg
    13681 ctatctccgg gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg
    13741 tattgccatt gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct
    13801 gcaacaaaac atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca
    13861 agccgccaga gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga
    13921 acgtcagggc gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatctct
    13981 aggcgtttat gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg
    14041 tttacccgcc tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgcc
    14101 agaaatgctc gatgaattac tggcaaccgg agcggttatc tggtcgggac aaaaaaaagc
    14161 tgggtgaaga tgacggcctg gtggcactgc atctacagaa atatgccgca gaatcgttca
    14221 ctcccgccga agcggatcag gcgaatcgtt cggcgctgca gcaagcgata atcgctgttc
    14281 tggctgacgg aggagcctgg tttgcacaac aaatcagcca acggatacgc gataaaatcg
    14341 tcgaatcggt tgattcctcg gcactgcaag aggcgttatg ggcgctggtc tggcaaggcg
    14401 tcattaccag cgatatttgg gcaccgttac gcgccctcac ccgcagcagt tccaacgcac
    14461 gcacctcaac tcgccgcagt caccgggctc gtcgtgggcg tcctgtctat gcacaacccg
    14521 tctcgccgcg ggtatcttac aacacaccaa atctggctgg acgctggtcg ttattgcagg
    14581 tggagccact aaacgatacc gaaaggatgc tggcgctggc ggaaaatatg ctcgaccgct
    14641 acggcatcat cagtcgtcag gcggtgatag ccgaaaatat ccctggcggg tttccatcga
    14701 tgcaaacact ttgtcgaagt atggaagact ccgggcgaat tatgcgaggt cgttttgtag
    14761 aaggtctggg tggcgcgcaa ttcgctgaac gtctgactat tgaccgattg cgcgatctgg
    14821 cgacacaagc cacgcaaaca cgccactata caccagtggc gctctccgcc aacgatccgg
    14881 ctaatgtgtg gggaaatctt ctgccctggc ctgcacatcc ggcaacgctg gtaccaacgc
    14941 gtcgggcggg tgcgctggtg gtcgtttctg gcggcaaatt gttactctat ctggcgcaag
    15001 gcggcaaaaa aatgctggtc tggcaggaaa aggaggaatt actcgcccca gagattttcc
    15061 aggcgctgac caccgcactg cgtcgcgaac caaggctgcg ctttacgcta acagaagtga
    15121 atgatcttcc ggtccggcaa acgccgatgt ttacgctgct gcgtgaggcg ggattttcaa
    15181 gttcgccaca agggctggat tggggatagt tgacatcctc cacgccctga aagacgtggt
    15241 tccctgctac gttcaagctg tcgcctgaat catttcggtg ggttcctgct tcaacgagcg
    15301 gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta aaatgttacg
    15361 agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga atttactttg
    15421 tgacaggcgg ttttctttcg ctgtatgacc acagcacgcg caac
//
LOCUS       DADLCQ010000095        15408 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000095 DADLCQ010000000
VERSION     DADLCQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.095"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1173)
                     /locus_tag="JQG95_002874"
     CDS             complement(<1..1173)
                     /locus_tag="JQG95_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY5889373.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1372..1456)
                     /locus_tag="JQG95_002875"
     tRNA            complement(1372..1456)
                     /locus_tag="JQG95_002875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Leu,seq:caa)
     gene            1652..2671
                     /gene="ahr"
                     /locus_tag="JQG95_002876"
     CDS             1652..2671
                     /gene="ahr"
                     /locus_tag="JQG95_002876"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY5889374.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2800..4302
                     /locus_tag="JQG95_002877"
     CDS             2800..4302
                     /locus_tag="JQG95_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY5889375.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFVQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="JQG95_002878"
     CDS             complement(4421..5503)
                     /gene="lptG"
                     /locus_tag="JQG95_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY5889376.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMVIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="JQG95_002879"
     CDS             complement(5503..6603)
                     /gene="lptF"
                     /locus_tag="JQG95_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY5889377.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6870..8381
                     /gene="pepA"
                     /locus_tag="JQG95_002880"
     CDS             6870..8381
                     /gene="pepA"
                     /locus_tag="JQG95_002880"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY5889378.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8515..8958
                     /gene="holC"
                     /locus_tag="JQG95_002881"
     CDS             8515..8958
                     /gene="holC"
                     /locus_tag="JQG95_002881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY5889379.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8958..11813
                     /gene="valS"
                     /locus_tag="JQG95_002882"
     CDS             8958..11813
                     /gene="valS"
                     /locus_tag="JQG95_002882"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY5889380.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDICESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11867..13063)
                     /locus_tag="JQG95_002883"
     CDS             complement(11867..13063)
                     /locus_tag="JQG95_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY5889381.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICMVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLLFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13256..13759
                     /locus_tag="JQG95_002884"
     CDS             13256..13759
                     /locus_tag="JQG95_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5889382.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="JQG95_002885"
     CDS             complement(13805..14221)
                     /gene="rraB"
                     /locus_tag="JQG95_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY5889383.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14383..15396
                     /gene="argF"
                     /locus_tag="JQG95_002886"
     CDS             14383..15396
                     /gene="argF"
                     /locus_tag="JQG95_002886"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY5889384.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3975 a         3932 c         3966 g         3535 t
ORIGIN      
        1 caacctacgc tccccaagat gttccgcctt atggatgtaa gctgctcgca cagagctacg
       61 ctcctggtga ctcatctgcc gctctaccgc atccctcgac cataatcccg actcaatcaa
      121 cgaactacaa gccatcgtcc tgaagccatg cccacaaacc tcgaccttcg tgtcataccc
      181 cattacgcgc aaagccttgt tcaccgtatt ctcactcatc ggcttacgtg gatcgtgatc
      241 gcccacaaaa atcagctctc gatttccgct catgcttttg atcttttcca aaatacttag
      301 ggcctgccgc gacaaaggta caaggtgtgg tgtccgcatc ttcgagccac gctgagaatg
      361 cttgactcct tccagttgct cacgctcacc tgggattgtc cacatagccg tttcaaaatc
      421 tatttctgac cagcgggcaa aacgcagctc gctcgaacgg atgaaaacca ataaagtgag
      481 ttcaaccgcc agtcgagtta acggtctgcc agaatagtga tctatgcgat gaagtaattc
      541 aggtatacgg ttcagttcaa gagctgcacg gtgctgtctt ttcgccgtag caaccgcacc
      601 tgcaatctct tgcgcggggt tgtagtcgat taagccgctc tgaacggcaa agcgcataat
      661 cgcggtagta cgctgctgta aacgggcggc aacttcgagc cgcccggatg actcgactgc
      721 tttaatgggt acaagaagat cccgcgtctt cagttccgca atgttccgct taccgatagc
      781 agtaaagaga ttatcctcca gacttttcaa aacacgagcg ctatgcgatg ccgaccactt
      841 ctgattgctg gcgtgccagt ctctggcgac tacttcaaac gttatcgcct cttgttcctg
      901 ttctacctta acagctttct tgttttcact gggatcgaca ccattagcta aaagcttacg
      961 agcctcatcc cggcgtgccc tggcatccgc caacgacact tcggggtatt tccccaatgc
     1021 cagcatcttc tctttgccgc caaagcgata acgtagccgc cagtatttgg agccgttagg
     1081 gtgaaccagc aaaaccatgc cttcaccgtc ggtcagctta taggcttttg cttcaggctt
     1141 agccgaacga accttcacat cactcagagc catgatgagt atcctttcaa gggttctgtg
     1201 tgggtacaaa cattatcgaa ccgggaaata cccgcagttg tacccgcatc agtaagttga
     1261 tgtagattga atcaggttga cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac
     1321 tggattttag gcacaaaaaa agacgtccgt tgacgtctat tgatgttccg atggtgccga
     1381 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1441 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1501 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1561 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1621 ttcccaaaca acaccagaga aggccccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1681 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     1741 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     1801 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtagt
     1861 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     1921 ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg
     1981 cgagcaaggt gcggtgccga caattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2041 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2101 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2161 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2221 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2281 gatgggtgcc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2341 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2401 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2461 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2521 ttatgagctg cgaaagctga tgcgctttgc cgcccgcagc aaggttgcgc cgacaaccga
     2581 actgttcccg atgtcgaaaa ttaacgacgc cattcagcat gtacgcgatg gtaaagcgcg
     2641 ctaccgagta gtcctgaaag ccgacttctg atccctttgc cgggtgctgt cgcccggcac
     2701 ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag agtgcacagc
     2761 cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc cctgttaatt
     2821 gcccgcacgc cggacacaga actgttttta ctgccgggaa tggctaaccg tcacgggctg
     2881 attactggcg caacggggac gggtaaaacc gttacgctgc aaaaactggc agagtcattg
     2941 tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac cggtatcgcg
     3001 caggcaggaa cggcgtcgga aaaactgctc acaaggctta aaaatatcgg cgtcaatgac
     3061 tggcaaccgc atgccaatcc ggtggtggtg tgggatatct ttggcgagaa aggccacacg
     3121 gtgcgggcga cggtttcgga tctggggccg ctgttgctgg cgcgactgtt gaatctcaac
     3181 gatgtgcaat ctggcgtgct gaatattatc ttccgcattg cggacgatca gggattgttg
     3241 ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa cgccaaatcc
     3301 ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca gcgcggatta
     3361 ctgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct ggatatcaaa
     3421 gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag cgccgagaaa
     3481 ctttatcaga tgccgaaact gtacgccgcc agcctgctgt ggatgctttc agagttgtat
     3541 gaacaattgc cggaagcggg cgatctggag aaaccaaaac tggtgttttt cttcgacgag
     3601 gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga gcaggtgata
     3661 aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc gtctgatatt
     3721 ccggataatg tgttcgtcca gctcggtaat cgtgttcagc acgctttgcg ggcttttacg
     3781 ccaaaagatc agaaagcagt gaagacagcg gcgcaaacca tgcgggccaa tccggcattt
     3841 gataccgaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc gtttctcgat
     3901 gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga tcgcaccttg ttcgcgaatg
     3961 gggccggtga cggaagatga gcgtaatggc ctgattaatc actccccggt gtatggcaaa
     4021 tatgaagatg aggtggaccg cgagtccgcc tatgagatgc tgcaaaaagg ctttcaggcc
     4081 agtaccgagc agcaaaataa tccccccgtg aaagggaaag aggtggcggt ggatgacggt
     4141 attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg cgggaagaaa
     4201 gatggtgtgg tgcaaacaat ggcgaaaagc gccgctcgcc aggtgacgaa tcagattgta
     4261 cgcgggatgt tggggagttt gctggggggg agaaaaagat aagtcagacg tgatctgtgc
     4321 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg ttcgaactcg tatttgactt
     4381 tgtcaacaat aataaaggga gctttcgctc ccttgttaga ttacgatttt ctcattaaca
     4441 gccacaggct gattaagaag aagctggcac ttggcaacag tgcgccgatg atcggcggga
     4501 tgccgtaaac caacgtcagc gggccgaaga tctggtccag tacgtagaag acaaaaccga
     4561 agctgatacc tgtgaccaca cgcacgccca tcggtacgct acgcagtggg ccaaagataa
     4621 acgacagcgc catcagcatc atcaccgcca cggatagcgg ctggaagatt ttgctccaca
     4681 tgttgagctg ataacgtccg gcatcctgac cgctcgactt cagatacttc acgtagttgt
     4741 gcaaaccact gatggagagt gcatccgggt ccagcgccac cacgcccagt ttgtctggcg
     4801 tgaggtcggt tttccaggtg ccgctcaccg tctgcgaacc ggtaatctgt ttcggattgg
     4861 tcagatcaga ttcatcaacc tgcgacagac gccagacttt atgttccggg tcaaacttcg
     4921 cggtagcggc ataacgtacg gattgcagac gacgattctc gttaaaggca taaatactga
     4981 tgccacctaa cacctcgtca cctttaaccc gctcaatgta gacgaagttg ttgccatctt
     5041 tcgcccataa accttgctgg gtagagagca acgagccgcc gtacatcgcc tgcgcacggt
     5101 agttacgcgc catctgttcg ccctgcggcg cgacccattc gccaatcacc atcgtcagca
     5161 agaccagtgg aatggcggtt ttcatcaccg acagcgctac ctgcatacgg gtaaaaccag
     5221 aagcctgcat caccaccagt tcgctgcgct gcgccagcat cccaagacca agcaacgccc
     5281 caagaagagc cgccatcggg aagaagatct gcacatcttt cggcacgctc agcaaggtat
     5341 acattcctgc gcctaacgcg tcgtaactcc cctgcccggc ttttttcagc tgatcgacaa
     5401 atttgataat gcccgacagc gacaccagca tgaacagcgt catcatgatg gtggtgaaaa
     5461 tagttttacc gatatagcgg tcaagtacgc caaaaggttg catcacaccg ctcctttacg
     5521 cgaaaaactg gcgcgcaggc ggcggaccgg cacggtgtcc caaaggttga gaacaatcgc
     5581 caaagccaga taaatcaggt taacggtcca catccacagt gtcgggtcca gcttaccttt
     5641 accgccgttc gatttcaggg aggtctggat caggaagaaa agtagataca gcagcatggc
     5701 tggcagcatc gacagtacgc gtccctgacg cgggttaacc acgctcaacg gtacaaccat
     5761 aagtgccatc ataaacacgg tgaataccaa cgtgatacgc cagttcagtt ctgcgcgagc
     5821 acgatcggtg tcagtgttcc acaatgtgcg catgtccatc tggtcggtat cgttcgggtc
     5881 gagcgccacc gcctggtgac caatgatcgc ctgataatcc tggaaatccg taatgcggaa
     5941 atcacgtaac agtgcagtgc cttcgaagcg cgttccctgg ttgagagtga cgacctggga
     6001 gccgtcgcgc agctgggtta aatgtccgga atcggccacc accacagaag gacgtgcatt
     6061 accttttggt cgaatttgtg cgaggaacac atctttgaaa tcgctgccgt caacgctttc
     6121 gatgaacagc accgagctgc cattagtcgc ttgctggaat tgcccttgcg ccagcgccgc
     6181 catgccaggg ttcgctttcg cttctgctaa cacttcatcc tgatgacgcg atgaccacgg
     6241 tcccgcccac atcacgttaa ccgccgcgac gattgccgtg aataccgcaa ggatcattgc
     6301 cgctttcacc agaaccgctt tgctcaagcc gcaggcatgc attaccgtaa tttcactttc
     6361 ggtatacagt ttgcccagcg tcatcagcag cccgaggaac aggcttaatg gcaggataag
     6421 ctgcgccatt tccggcacgc ccaacccgag aagggagagc accagattcg ccggaatatc
     6481 gccgtcaacc gctgcgccga ggatcctcac taacttttga cagaagaaga tcaaaagcaa
     6541 gatgaagagt atcgccagct ggcttttgag cgtctcccgc accagatatc ttatgattat
     6601 cactttaaat acgcccgtaa aaactcgtct tttgcaggat tttagcttgt ttcatggctt
     6661 aaacgtcatt tattctcttg agtcgtcgaa atcgtcgcta agataattat actcaacgga
     6721 ttcacctctc agattttgtt ctgacgtgcc aatgccgtaa taacgttaag attaacacga
     6781 agtcatcgca acagcggaca tgagttacga aagcttgcaa ttctatctgt agccaccgcc
     6841 gttgtcttta agattcagga gcgtagtgca tggagtttag tgtaaaaagc ggtagcccgg
     6901 agaaacagcg gagtgcctgc atcgtcgtgg gcgtcttcga accacgtcgc ctttctccga
     6961 ttgcagaaca gctcgataaa atcagcgatg ggtacatcag cgccctgcta cgccggggcg
     7021 aactggaagg aaaaccgggg cagacattgt tgctgcacca tgttccgaat gtactttccg
     7081 agcgaattct ccttattggt tgcggcaaag aacgtgagct ggatgagcgt cagtacaagc
     7141 aggttattca gaaaaccatt aatacgctga atgatactgg ctcgatggaa gcggtctgct
     7201 ttctgactga actgcacgtt aaaggccgta acaactactg gaaagtgcgt caggctgtcg
     7261 agacagcaaa agagacgctc tacagtttcg atcagttgaa aacgaataag agcgaaccgc
     7321 gtcgtccgct gcgtaaaatg gtgttcaacg tgccgacccg ccgtgaactg accagcggtg
     7381 agcgcgcgat ccagcacggt ctggcgattg ccgccgggat aaaagcagcg aaagatctcg
     7441 gtaatatgcc gccgaatatc tgtaacgccg cttatctcgc ctctcaggcg cgccagctgg
     7501 ctgacagcta cagcaagaat gtcatcaccc gcgttatcgg cgaacagcag atgaaagagc
     7561 tgggaatgca ctcttatctg gcggtcggtc agggttcaca aaacgaatcg ctgatgtcgg
     7621 tgattgagta caaaggcaac gcgtcggaag atgcacgccc aatcgtgctg gtgggtaaag
     7681 gtttaacctt cgactccggc ggtatctcca tcaagccttc agaaggcatg gatgagatga
     7741 agtacgatat gtgcggcgcg gcggcagttt acggcgtgat gcgtatggtc gcggagctgc
     7801 aactgccgat taacgttatt ggcgtgctgg caggctgtga aaacatgcct ggcgggcgtg
     7861 cctatcgtcc gggcgatgtg ttaaccacca tgtccggtca aaccgttgaa gtgctgaaca
     7921 ccgatgccga aggccgcctg gtactgtgcg acgtgttaac ttacgttgaa cgttttgagc
     7981 cggaagcagt gattgacgtg gcgacgctga ccggtgcctg cgtgatcgcg ctgggtcatc
     8041 atattactgg tctgatggcg aaccataatc cgctggccca tgaactgatt gccgcgtctg
     8101 aacaatccgg tgaccgcgca tggcgcttac cgctgggtga cgagtatcag gaacagctgg
     8161 agtccaattt tgccgatatg gcgaacattg gcggtcgtcc tggtggggcg attaccgcag
     8221 gttgcttcct gtcacgcttt acccgtaagt ataactgggc gcacctggat attgcaggaa
     8281 ccgcctggcg ttctggtaaa gcaaaaggcg caaccggtcg tccggtagcg ttgctggcac
     8341 agttcctgtt aaaccgcgct gggtttaacg gcgaagagta agtttgcgtt ttaccctcac
     8401 cctaaccctc tccctaaggg agagggaacc gatcgagcgc aatagaaatt cattgctcat
     8461 ctttcagggt gaggaatgca gcaatattta aatccaccac aagaagcccc atatatgaaa
     8521 aacgcaacgt tctaccttct ggacaatgac accaccgtcg atggcttaag cgccgttgag
     8581 caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt actcatcgcc
     8641 tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa
     8701 agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcagtgcgcc ggtggagatc
     8761 gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct gcgaacaagc
     8821 tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta tgaagattct
     8881 ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt caacctgaat
     8941 acggcaacct ggaaataatg gaaaagacat acaacccaca agatatcgaa cagccgcttt
     9001 acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc caggaaagtt
     9061 tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt cacgccttcc
     9121 agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa aacaccctgt
     9181 ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag cgcaagattg
     9241 ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc gacaaaatct
     9301 gggaatggaa agcggaatct ggcggcacca ttacccgtca gatgcgccgt ctcggcaact
     9361 ccgtcgactg ggagcgtgaa cgcttcacca tggacgaagg cctgtccaat gcggtgaaag
     9421 aagttttcgt tcgtctgtat aaagaagacc tgatttaccg tggcaagcgc ctggtaaact
     9481 gggacccgaa actgcgtacc gctatttctg acctggaagt ggaaaaccgc gaatcgaaag
     9541 gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag
     9601 attatctggt ggttgcgacc actcgcccgg aaaccctgct gggcgatacc ggtgtagctg
     9661 ttaacccgga agatccgcgt tacaaagatc tgattggcaa atatgtcatt ctgccgctgg
     9721 ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa ggcaccggct
     9781 gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa cgtcacgccc
     9841 tgccgatgat caacatcctg acctttgacg gtgatatctg tgaaagcgcc caggtgttcg
     9901 ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagag ttccagaaac
     9961 tcgagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgatgcactc ggcctgctgg
    10021 aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc gtggttatcg
    10081 aaccgatgct gaccgaccag tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg
    10141 aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac atgtacttct
    10201 cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg ggtcaccgta
    10261 tcccggcatg gtatgacgaa gcgggtaatg tttatgttgg ccgcaacgaa gaagaagtgc
    10321 gcaaggaaaa taacctcggt gccaacgttg ccctgcgtca ggacgaagac gttctcgaca
    10381 cctggttctc ttctgcactg tggaccttct ctacccttgg ctggccggaa aataccgacg
    10441 ccctgcgtca gttccaccca accagcgtga tggtatctgg tttcgacatc atcttcttct
    10501 ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc
    10561 aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa ggtcagaaga
    10621 tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt atttcgctgc
    10681 cagaactgct ggaaaaacgc accggcaata tgatgcagcc gcagctggcg gacaaaatcc
    10741 gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc gatgccctgc
    10801 gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat atgaagcgtc
    10861 tggaaggcta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga
    10921 acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctatcg ctggcggacc
    10981 gctggattct ggcggagttc aaccagacta tcaaagcgta ccgcgaagcg ctggacagct
    11041 tccgcttcga tatcgccgca ggcattttgt atgagttcac ctggaaccag ttctgcgact
    11101 ggtatctgga gctgaccaag ccggtaatga acggtggaac agaagccgaa ctgcgcggta
    11161 ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat ccgatcattc
    11221 cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc actgccgaca
    11281 ccatcatgct gcagccgttc ccgcagtatg atgcatctca ggttgatgaa gccgcactgg
    11341 ccgacaccga gtggctgaaa caggcgatcg ttgctgtacg taacatccgt gcagaaatga
    11401 acatcgcgcc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac
    11461 gtcgcgtaaa tgaaaaccgt ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg
    11521 tgctgcctgc cgatgacaaa ggtccggttt ccgttaccaa gatcatcgac ggcgcagagc
    11581 tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgc ctggcgaaag
    11641 aagtggcgaa gatcgaaggt gaaatcagcc gcatcgagaa caagctggca aatgaaggct
    11701 ttgtcgcccg cgcaccggaa gcggttatcg cgaaagagcg tgagaagctg gaaggttatg
    11761 cagaagcgaa agccaagctg attgaacagc aggctgttat cgccgcactg taatcaacac
    11821 agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac agaaatggta
    11881 aagaaggcat aactccccga gaaatactgg ctaagaagcc tttatctggt tgttcttctt
    11941 tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt gcctgccagt
    12001 caatcatcca tattttcagc agtgggtaag ccagaccacc cgtaatcccg gatatcacca
    12061 ccaacgcaca catgcgataa agcataccgt ggtacgttaa agttgagcga aaacgaatac
    12121 gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc gttaaataac
    12181 tggtgctaac agcaatccca aaataataaa ttaactgcgc gattatcatt ttacgttgca
    12241 tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa ctatcatact
    12301 catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaagt aataaagcta
    12361 aaaatgcgag gatggcatat tttatacaat acgcagtatc taatttcacc ttgaaacgat
    12421 gtataccgaa actggtattg ctccagagaa aactcattac caggctatat aaagtaccat
    12481 taaaaatacc aatggaaaca ataccaacta ttgccatcag aaccacagat ataataacac
    12541 tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc cccatcccaa
    12601 tggccattaa aatcggcaag aaaaaggtta cccaccagaa ccctttcaaa gagcaattaa
    12661 aactaaatct tacgccgttg agactggtca ttaaggcctg ataacgcagt ccttttgctg
    12721 ccataaatat aagcaaaacc aacagtaaca aagtcaaaac acagccaaca agcggcatat
    12781 ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa
    12841 aaacattccc accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat
    12901 aaagataacg cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg
    12961 ttaacaacac attcaccata caaataagaa aatatctctc acctgtacca tgaaaaacaa
    13021 aagaatggga cggaacatcc atttcattaa taacttgagc cataaaaatc ccctgaatta
    13081 aaaaatccat tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata
    13141 ctcttatcag taataggctg gcaaaaactt tattgcttta taagacgctt gcgcacgtaa
    13201 gaagagagtg gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa
    13261 taacattgcg ccgcaatcac ctgtaatgcg tcgcctgacg ctgcaggata atcctgctat
    13321 cgccagcgtc atccgtcagg tttacgcaga gtatggtctt accgccgata aaggctacac
    13381 cgttgccgat ccgaatctcg acgaactgta tcaagtgtac agccagcctg gccatgctta
    13441 ttgggtggtt gaatacaatg gcgaagtggt cggcggcggc gggattgcgc cattaacagg
    13501 tagtgaagcg gacatttgcg aactgcagaa aatgtacttt cttcccgcta ttcgcggtaa
    13561 agggctagcg aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa
    13621 acgctgctat ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt
    13681 gggctttgag catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg
    13741 gatgctacgc gaactctaaa aaaatgcccg ctagtatgtg ctatcgggca ttttaatcaa
    13801 ttaattagtg gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccatctt
    13861 cgccgttagg atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg
    13921 ccagcgtcat cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc
    13981 tgaggatgtc gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg
    14041 tcacttcgta accgagttta aacgcttcaa ctgccgcttt ttccagagtt tccagatcgt
    14101 ctgcggaaag atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta
    14161 attcttcaat aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca
    14221 tggctcattc ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc
    14281 tccacctttg tgacaaagat ttatgcttta gacttgcaaa tgaataataa tacacataaa
    14341 ttgaatttta attcattgag gcgttagcca caggagggat ctatgtccgg gttttatcat
    14401 aagcatttcc tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta
    14461 gccgcgaagc tgaaagctga taagaaaagc ggtaaagaag aagccaaact cactggtaaa
    14521 aacatcgcgc tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc
    14581 gcatatgacc agggggctcg cgttacttat cttggcccaa gcggtagtca gattggtcat
    14641 aaagagtcga ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat
    14701 cgcggctatg gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg
    14761 aacggcctga ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag
    14821 gagcatttac ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt
    14881 aacaacatgg gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg
    14941 gtcgcgccac aggcatgctg gccagaagct gcgctggtta cggaatgccg cgccctggca
    15001 caacaaaatg gcgggaatat tacgctgacg gaagatgtag ctaaaggagt tgaaggtgct
    15061 gactttatct ataccgatgt atgggtgtcg atgggtgaag caaaagaaaa atgggctgag
    15121 cgcattgcat tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac
    15181 ccggaggtca aattcctcca ttgcctgccc gcgtttcatg acgaccaaac gacgcttggc
    15241 aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc
    15301 gaatctgccg ccagcattgt atttgatcag gcggaaaacc gcatgcatac catcaaagcg
    15361 gtgatggtcg cgacgctcag taaattaaat aattaatatc tctataaa
//
LOCUS       DADLCQ010000096        15344 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000096 DADLCQ010000000
VERSION     DADLCQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.096"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQG95_002887"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="JQG95_002887"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY5889385.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNASKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQG95_002888"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="JQG95_002888"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY5889386.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQG95_002889"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="JQG95_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY5889387.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="JQG95_002890"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="JQG95_002890"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY5889388.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="JQG95_002891"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="JQG95_002891"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY5889389.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="JQG95_002892"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="JQG95_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY5889390.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="JQG95_002893"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="JQG95_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY5889391.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="JQG95_002894"
     CDS             complement(8064..9206)
                     /locus_tag="JQG95_002894"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY5889392.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="JQG95_002895"
     CDS             complement(9215..10228)
                     /locus_tag="JQG95_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY5889393.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQG95_002896"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="JQG95_002896"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY5889394.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQG95_002897"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="JQG95_002897"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY5889395.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQG95_002898"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="JQG95_002898"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY5889396.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="JQG95_002899"
     CDS             complement(12826..13950)
                     /locus_tag="JQG95_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY5889397.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQG95_002900"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="JQG95_002900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY5889398.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4379 a         3445 c         3146 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttgg aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctgcc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgctaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcagca agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DADLCQ010000097        15098 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000097 DADLCQ010000000
VERSION     DADLCQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.097"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            5..178
                     /locus_tag="JQG95_002901"
     CDS             5..178
                     /locus_tag="JQG95_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY5889399.1"
                     /translation="MTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEAS
                     FSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="JQG95_002902"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="JQG95_002902"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY5889400.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRYLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="JQG95_002903"
     CDS             1531..2478
                     /locus_tag="JQG95_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY5889401.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="JQG95_002904"
     CDS             2548..3354
                     /locus_tag="JQG95_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5889402.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="JQG95_002905"
                     /pseudo
     CDS             3364..3771
                     /locus_tag="JQG95_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
     gene            3771..4265
                     /locus_tag="JQG95_002906"
     CDS             3771..4265
                     /locus_tag="JQG95_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY5889403.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="JQG95_002907"
     CDS             4331..5128
                     /locus_tag="JQG95_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY5889404.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="JQG95_002908"
     CDS             5139..5927
                     /locus_tag="JQG95_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY5889405.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /locus_tag="JQG95_002909"
     CDS             5979..7208
                     /locus_tag="JQG95_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5889406.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="JQG95_002910"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="JQG95_002910"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY5889407.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="JQG95_002911"
     CDS             complement(8370..10001)
                     /locus_tag="JQG95_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY5889408.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="JQG95_002912"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="JQG95_002912"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY5889409.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="JQG95_002913"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="JQG95_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY5889410.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELSQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3591 a         3982 c         3936 g         3589 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatagcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggagaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtagaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca tggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggcgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct agtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gtacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattaa gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 ctcaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg tcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttca
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaa
//
LOCUS       DADLCQ010000098        15050 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000098 DADLCQ010000000
VERSION     DADLCQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.098"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(592..1533)
                     /gene="fabY"
                     /locus_tag="JQG95_002914"
                     /gene_synonym="yiiD"
     CDS             complement(592..1533)
                     /gene="fabY"
                     /locus_tag="JQG95_002914"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY5889411.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="JQG95_002915"
     CDS             complement(1578..2015)
                     /gene="dtd"
                     /locus_tag="JQG95_002915"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY5889412.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2012..2884)
                     /locus_tag="JQG95_002916"
     CDS             complement(2012..2884)
                     /locus_tag="JQG95_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY5889413.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="JQG95_002917"
     CDS             complement(2878..3477)
                     /gene="yihX"
                     /locus_tag="JQG95_002917"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY5889414.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3576..4361)
                     /locus_tag="JQG95_002918"
     CDS             complement(3576..4361)
                     /locus_tag="JQG95_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY5889415.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="JQG95_002919"
     CDS             complement(4395..5291)
                     /gene="yihV"
                     /locus_tag="JQG95_002919"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY5889416.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGLPDCDQTRSFLSLFV"
     gene            5459..6355
                     /gene="yihU"
                     /locus_tag="JQG95_002920"
     CDS             5459..6355
                     /gene="yihU"
                     /locus_tag="JQG95_002920"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY5889417.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGHGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6379..7257
                     /gene="yihT"
                     /locus_tag="JQG95_002921"
     CDS             6379..7257
                     /gene="yihT"
                     /locus_tag="JQG95_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY5889418.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7274..8515
                     /gene="yihS"
                     /locus_tag="JQG95_002922"
     CDS             7274..8515
                     /gene="yihS"
                     /locus_tag="JQG95_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY5889419.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8629..9555
                     /locus_tag="JQG95_002923"
                     /pseudo
     CDS             8629..9555
                     /locus_tag="JQG95_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
     gene            9754..11790
                     /gene="yihQ"
                     /locus_tag="JQG95_002924"
     CDS             9754..11790
                     /gene="yihQ"
                     /locus_tag="JQG95_002924"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HAY5889420.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11836..13210
                     /locus_tag="JQG95_002925"
                     /pseudo
     CDS             11836..13210
                     /locus_tag="JQG95_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13253..14656
                     /locus_tag="JQG95_002926"
     CDS             13253..14656
                     /locus_tag="JQG95_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5889421.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14724..>15050
                     /locus_tag="JQG95_002927"
     CDS             14724..>15050
                     /locus_tag="JQG95_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY5889422.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3699 a         3874 c         3744 g         3733 t
ORIGIN      
        1 aaaccatgct tcagttcgtt aacacaatcg cacagttgct cctcaccttc cattttaagg
       61 caaagtgaaa attgatttaa tagcgtttgc ttcggtgatt tattactcat tagttgtttt
      121 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      181 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      241 gtaaatgaat gatactgaca atgtatgcca gcccattatt tttgcataat atttgtgagc
      301 tatagatttt aaaaatattt atgaaaaaag tatggcacca gagggggttt tgactatcaa
      361 aaatcgctca aacctgaact aatatgatga gttaagcatt atctgcaata attgccactc
      421 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      481 caacgaagaa taaaaattta taaagttctt attcaatata accggaatag tcagggactg
      541 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      601 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      661 ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct gcacccgtgc
      721 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt cggctaccgc
      781 atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca gaataatcgt
      841 tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg tgagcgtcgc
      901 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc cggtttccgg
      961 catggtggtg ataaattttt gcccggtata ttgttgaata cgcacgccca ttttttcact
     1021 aagcgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1081 aatatcatcc agagtggcga cgggcttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1141 tggcgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1201 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1261 catcgccatc agcgttccta accctttgtc ctgcacgtcc gggtgaacga ccataaagcg
     1321 aatggacgct tcattgtcgg cattaatata caatcggcct accgccacca gattaccctg
     1381 ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1441 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1501 ttcttctgtt tgtggaacct gaaggtgata catagcgata ctctctcttg tttcccgtga
     1561 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1621 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1681 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     1741 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     1801 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     1861 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     1921 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     1981 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2041 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2101 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2161 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2221 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2281 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2341 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2401 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2461 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2521 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2581 tcatcttgtt ggaattagca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2641 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     2701 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgaca
     2761 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     2821 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     2881 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     2941 aatgcctagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3001 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3061 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3121 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3181 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3241 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3301 ctctgcgaac gcttcatcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3361 atgaaaactc ttcttaagcg atgccagcgg aatacgcgtt aaatcgctcc aggctcccag
     3421 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3481 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3541 gcaaggtatg aagccaggtt aacggtcaca aaaatctacg cgtcttcctg gggaagaatg
     3601 atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag ctcgtcggta
     3661 aagagcgcag tgacctgtgc cacgttacca atttcaaccg ccgccgaagc atgatactta
     3721 gtgtgatcgg cgaccagcag aatatttctc gcgtgcgcca tcatcgtttt caccacgtta
     3781 gcttcgttta catcaaactc catcaacgcg ccatcgctct caatcgcccc aacgcttgtt
     3841 accagataat cagcacgaaa atcagccaca aaggacgccg ctgaagggcc aatgatcccg
     3901 ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg gttgtggtaa
     3961 agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg gttaagtaac
     4021 gcccgggcaa catgctcaac agtcgtacca atggtgataa atattgttga accatcagga
     4081 atatagtctg ccaccgcttc ggcaatcgct ttttttttct cggtttgcga aacctcacgc
     4141 tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg atggcgcgta
     4201 atcaggcctt gctcgcttaa tttacgaata tcccggcgga ccgtctgcgt ggagacatcc
     4261 agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag catgaggagt
     4321 tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc tcgaaaaacc
     4381 atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc acagtcaggg
     4441 agcccggctc gtccaccggg acgtgtgcat tttaacgccg ctacaccgct ggcgaagcgg
     4501 actgactccg ctaaatcccc acttgttgcc agcgccaccg ccaaagcgcc gtgaaaaaca
     4561 tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg ctgacgccca
     4621 ccattttcca gccagtcgca gcctgcgcta ccctgggtca catagacatg accatttgtg
     4681 agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa gcgcgccaga
     4741 cccggttctg aaaaggccgc gtgatcgctt aatgccacca gctcactgat atcctgcggc
     4801 gtaatgtccc cgtccagaac ggtcatcaca cccgcctgac gggccagggt gaaggctttt
     4861 ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga gaaatcaatt
     4921 tcctccaacc actctgcgtc aggcagcaga tccgggctgg ggtagttaat gattatccgc
     4981 tcgcctttgg tatccaccat gatggcggat tgcgaagatt tcgcctggtt ataccgtttg
     5041 gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt gcccgcgtcg
     5101 tcatcaccta cgcgaccaat aaaatcgacc tgcgccccca gccttgccgc cgcaaccgct
     5161 gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta tttaccgctc
     5221 tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc tacacaagca
     5281 acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat ttcaccaatt
     5341 gtttaaaaag tgacggctgt caattttttg acgataaatt acaaattcga tcaaaaacag
     5401 acaagcaaag catgtcataa atgacaaaaa gtgacatcac tgtattcagg agagggttat
     5461 ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga gcaatttatt
     5521 gcagcaaggg catcaacttc gcgtctttga tgtgaacgcc gaagctgtgc ggcatctggt
     5581 agacaaaggt gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg ccgaatttat
     5641 cattaccatg ttgccgaatg gcgatctggt gcgcaacgtg ttgttcggtg aaaacggcgt
     5701 ttgcgaaggc ttatctaccg atgcgctggt cattgatatg tccaccatcc atccgctgca
     5761 aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg atgttccggt
     5821 aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg gcggcaccgc
     5881 tgaacaagtt gaacgtgcca caccgatcct gatggcgatg ggcagtgagt tgatcaacgc
     5941 tggcggtccg ggcatgggga tccgcgttaa gctcatcaac aactacatga gcatcgcgct
     6001 caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc ccttcgatgt
     6061 tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggt cacttcacca cttcctggcc
     6121 aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg cccataagga
     6181 tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc tgggggccgc
     6241 ctcacgggag gtttatagcc aggcgcgcgc agcgggtcac ggtcgccagg actggtccgc
     6301 cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa tgtaacgact
     6361 ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg cgcatcgggc
     6421 ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct
     6481 ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag
     6541 gccctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg
     6601 gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt
     6661 cctggcaacg gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag
     6721 atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat
     6781 gcgcagcaac gtctggatat ggtgaaagag ttcaacgaac tgtgccactc acacggtctg
     6841 gtaagcatca ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa
     6901 caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt
     6961 gaaatgccgc tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg
     7021 aatgaccata tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc
     7081 ccgcgtgccg tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca
     7141 gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca
     7201 ccgaaattac aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat
     7261 gaggataaaa ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga
     7321 aaccgaccgc atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt
     7381 aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat
     7441 gttgcacgtt tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga
     7501 tcacggtatt aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc
     7561 ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc
     7621 tctgctgggt gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga
     7681 ttacaccatt gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga
     7741 gtcctgggac gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca
     7801 cgcggtggaa gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg
     7861 cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt
     7921 taacgaacac ttcgataccc agtggaatcc gctgccggat tacaacaaag ataacccggc
     7981 gcatcgcttc cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat
     8041 gctgcacatc catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga
     8101 agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggagcgga
     8161 cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg
     8221 gcctatcgtc gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca
     8281 gtatgaaacc tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga
     8341 aaatggttcc tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga
     8401 cggcaaacag gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc
     8461 gcccggcatg gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat
     8521 ctgaaaatag ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga
     8581 ccgtccggtg cagtttttgg gtttgacatc agcctcacgg gaggctgtat gtcgttaatt
     8641 aaggtgcctt gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc
     8701 gcgggggatt atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt
     8761 cagggttgtc atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt
     8821 aaagtgctta ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag
     8881 gaatatcagt tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc
     8941 tggcgagact ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg
     9001 ccgcccagtt acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca
     9061 cacacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat
     9121 ggcgtgggta ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc
     9181 cagcttccgg caaactaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac
     9241 cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc
     9301 gaccacacct ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag
     9361 gcgctttcgg taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa
     9421 ttacagcgtc agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca
     9481 ggtatcgact tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac
     9541 ggacagagaa aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg
     9601 ctaaaggcaa cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa
     9661 gtttttatat atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca
     9721 acaatctcgt tctcaactct atggagatta gttatggata cgccacgtcc acagttatta
     9781 gattttcaat ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt
     9841 ttaacccata acaaagataa tccttgttta tggattggct caggtatagc ggatatcgat
     9901 atgttccgcg gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac
     9961 gccatcgtca gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt
    10021 agcgccacgc tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac
    10081 gacaacctta accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc
    10141 tacggctgcg gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg
    10201 accagtgaac aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc
    10261 aaagaaaatg cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc
    10321 acgcagaagt attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc
    10381 ccggaatacc atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct
    10441 gacacataca tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg
    10501 cccgactgga tttatgacgg ggtaacgctc ggcattcagg gcgggacgga agtgtgccag
    10561 aagaaactgg acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac
    10621 tggtctggga tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac
    10681 agcgaaaact acccgcaact ggattcacgc attaagcagt ggtatcagga gggcgtgcag
    10741 ttcctggcct atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca
    10801 caacacggct atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag
    10861 ttttacggcg gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg
    10921 atcaaaaaga acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat
    10981 ctgcccaccg acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctgg
    11041 cctgcgctgt gggcgaagtg taactacgaa gcccttgaag aaacgggcaa gctcggcgag
    11101 atcctcttct ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg
    11161 gcgggcgacc agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg
    11221 gcgctgtcgc tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc
    11281 accctgtttg agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc
    11341 ttcacgccga tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac
    11401 ggcgatgcgg aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa
    11461 ccttatctga aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg
    11521 ctgttcctgc attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta
    11581 ggccgcgaca ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat
    11641 ctgccggagg ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt
    11701 accgttaatg cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg
    11761 gcactgttcg cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc
    11821 aaagggagaa catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc
    11881 tttaaagaga aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc
    11941 ggtacgcttt atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc
    12001 ggcattatct ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt
    12061 atgttggatt cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat
    12121 gcgtcattcc cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc
    12181 accggtaaaa cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg
    12241 atgaactgct cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc
    12301 tcactggcgg catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga
    12361 ttcgtgccag ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc
    12421 acgctgtttt cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag
    12481 cgttatgtcg aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc
    12541 cgcgcaattg ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta
    12601 ggggcgttta acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat
    12661 cccatcctgt tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc
    12721 ctgatgcctg gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg
    12781 atttgggtgc tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg
    12841 gcgttctcct gcctggcgtt cttcggctca gcgcctgaac tgggcgctgg tttccgacac
    12901 cgtcgagtac ggcgagtggc gcaccggcgt gcgttcggaa ggcacggtct acaccggctt
    12961 caccttcttt cgcaaagtgt ctcaggcgct ggctggtttc ttccccggct ggatgctgac
    13021 gcaaattggc tatgtgccga acgtcgcaca ggctgaccac accattgaag ggttacgcca
    13081 gttgatcttc atctacccaa gcgcactggc ggtagtcacc attgtggcga tgggttgctt
    13141 ctacagcctg aacgagaaga tgtatgtccg cattgtggaa gagatagaag cccgtaaacg
    13201 cacggcgtaa taataataaa cgacgccctg cggggcgtta taaggagtga ttatgtctga
    13261 ccataatcca ctgacattaa aactgaatct gcgggaaaaa atcgcctatg gtatgggcga
    13321 cgtcggttcg aatttaatgc tctgcatcgg tactctgtat ctcctcaaac tttataccga
    13381 tgaattaggg atgcctgctt actatggcgg gattatcttt ctggtcgcga agttttttac
    13441 cgcgtttacc gatatgctca ccggattttt actcgactcg cggaaaaata ttgggcctaa
    13501 aggcaaattt cgtcccttta ttttatatgc agcagttccg gcggcgttaa ttgcaacgct
    13561 ccagtttatc gccaccacct tttgtttgcc ggttaaaacg acgattgcca ccacgctgtt
    13621 tatgatgttt ggactttcat acagtctgat gaactgctcg tatggtgcga tgatcccggc
    13681 aattaccaaa aacccgaatg agcgcgcgca gctcgcggct taccgccagg gtggtgcaac
    13741 catagggcta ttgatttgta ccgtggcgtt tattccgttg cagtcgcttt tttctgactc
    13801 aaccgtcggt tatgcctgtg cggcacttat gttctccatt ggcggcttta tttttatgat
    13861 gctgtgctac agaggcgtca aagagcatta tgtggacaca gcgccaaccg gacataaagc
    13921 cagtattctc aaatcttttt gcgcgatatt tcgtaatcca ccattgctgg ttttatgcat
    13981 tgctaacctg tgtactctgg cggcatttaa tatcaaactg gcgattcagg tctattacac
    14041 ccagtatgtg ctgaatgata ttaatttatt gtcgtggatg ggattcttca gtatgggatg
    14101 catcctcgtc ggcgtattac tggtgccagt aactgtaaaa tgttttggta aaaaacaggt
    14161 ttatttagct ggcatggtgc tatgggcggt gggtgatata ctgaattatt tctggggaag
    14221 taactctttc actttcgtca tgttctcttg tgtcgccttt tttggcacgg cctttgtaaa
    14281 cagcctgaac tgggcactgg taccagatac cgtagattac ggtgaatgga aaaccggtat
    14341 tcgtgccgaa ggttctgttt ataccggtta taccttcttt cgtaaaattt ctgccgcact
    14401 tgctggcttc ttgccaggca ttatgctgac gcaaattggt tatgttccca acatcgcgca
    14461 aagcgatgcg acattacagg gtttgcgtca gctcattttt atctggcctt gtgcactggc
    14521 aattattgct gcattgacga tgggattctt ttacacactc aatgaaaaac ggtttgcatt
    14581 gattattgag gaaatcaacc aacgcaaaaa taaagagatg gcgacagaaa aaaaaacggc
    14641 ttccgtaacg ttataaatgt caatagccgc tatttccatc ctggtgggtg gcggcctccc
    14701 tacgtttaaa aaatggactt attatgaaaa agattaatgc aataatttta ttatcttctc
    14761 tgacctctgc ctcggtattt gctggcgcat acgtcgaaaa cagggaggca tataatcttg
    14821 cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta
    14881 ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat ggctataatg
    14941 aaattgaagg ttggtatcct ttattcaaac caaccgataa attaaccatc cagcccggtg
    15001 gcttaattaa tgataagagt atcagttcgg gtggtgcagt gtatctggac
//
LOCUS       DADLCQ010000099        14854 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000099 DADLCQ010000000
VERSION     DADLCQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.099"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQG95_002928"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="JQG95_002928"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY5889423.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQG95_002929"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="JQG95_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY5889424.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="JQG95_002930"
     CDS             complement(1881..2234)
                     /gene="arsR"
                     /locus_tag="JQG95_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY5889425.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            2977..3060
                     /locus_tag="JQG95_002931"
     CDS             2977..3060
                     /locus_tag="JQG95_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY5889426.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="JQG95_002932"
     CDS             complement(3114..4466)
                     /gene="gorA"
                     /locus_tag="JQG95_002932"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY5889427.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4538..5380)
                     /locus_tag="JQG95_002933"
     CDS             complement(4538..5380)
                     /locus_tag="JQG95_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY5889428.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5583..7625
                     /gene="prlC"
                     /locus_tag="JQG95_002934"
     CDS             5583..7625
                     /gene="prlC"
                     /locus_tag="JQG95_002934"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY5889429.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7633..8385
                     /gene="rsmJ"
                     /locus_tag="JQG95_002935"
     CDS             7633..8385
                     /gene="rsmJ"
                     /locus_tag="JQG95_002935"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY5889430.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="JQG95_002936"
                     /pseudo
     CDS             complement(8434..9903)
                     /gene="dtpB"
                     /locus_tag="JQG95_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="JQG95_002937"
     CDS             complement(10221..10655)
                     /gene="uspA"
                     /locus_tag="JQG95_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY5889431.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11049..11384
                     /gene="uspB"
                     /locus_tag="JQG95_002938"
     CDS             11049..11384
                     /gene="uspB"
                     /locus_tag="JQG95_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY5889432.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="JQG95_002939"
     CDS             complement(11455..12954)
                     /gene="pitA"
                     /locus_tag="JQG95_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY5889433.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            13186..14385
                     /locus_tag="JQG95_002940"
     CDS             13186..14385
                     /locus_tag="JQG95_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY5889434.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3699 a         3781 c         3911 g         3463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaagac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt
     2221 aacagaaatg acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat
     2281 aagtcaaaaa catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga
     2341 attttgagtt ggctattcat ttgaaaggcg gtttgaatca ggacgtaaaa aaaccgactt
     2401 tgcgtcggtt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     2461 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     2521 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     2581 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     2641 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     2701 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     2761 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     2821 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     2881 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     2941 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     3001 cttggggcgt taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga
     3061 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     3121 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     3181 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     3241 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     3301 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     3361 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     3421 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     3481 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     3541 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     3601 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     3661 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     3721 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     3781 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     3841 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     3901 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     3961 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     4021 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     4081 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     4141 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     4201 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     4261 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     4321 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     4381 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     4441 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     4501 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     4561 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     4621 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     4681 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     4741 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     4801 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     4861 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     4921 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     4981 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     5041 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     5101 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     5161 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     5221 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     5281 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     5341 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     5401 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     5461 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     5521 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     5581 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     5641 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     5701 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     5761 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     5821 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     5881 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     5941 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     6001 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     6061 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     6121 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     6181 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     6241 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     6301 gcacccgcgc ctccgatcaa ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg
     6361 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     6421 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     6481 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     6541 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     6601 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     6661 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     6721 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     6781 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     6841 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     6901 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     6961 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     7021 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     7081 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     7141 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     7201 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     7261 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     7321 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     7381 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     7441 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     7501 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     7561 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     7621 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     7681 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     7741 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     7801 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     7861 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     7921 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     7981 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     8041 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     8101 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     8161 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     8221 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     8281 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     8341 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     8401 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     8461 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     8521 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     8581 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     8641 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     8701 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     8761 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     8821 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     8881 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     8941 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     9001 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     9061 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     9121 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     9181 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     9241 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     9301 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     9361 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     9421 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     9481 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     9541 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     9601 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     9661 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     9721 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     9781 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     9841 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     9901 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     9961 gaaaaagttt acatctgtca gagattctta atattgatgc ggtaaataga ttaatacact
    10021 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
    10081 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
    10141 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
    10201 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
    10261 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
    10321 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
    10381 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
    10441 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
    10501 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
    10561 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
    10621 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
    10681 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
    10741 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
    10801 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
    10861 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
    10921 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
    10981 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
    11041 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
    11101 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
    11161 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
    11221 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
    11281 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
    11341 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
    11401 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
    11461 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
    11521 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
    11581 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
    11641 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
    11701 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
    11761 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
    11821 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
    11881 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
    11941 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
    12001 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
    12061 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
    12121 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
    12181 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    12241 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    12301 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    12361 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    12421 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    12481 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    12541 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    12601 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    12661 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    12721 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    12781 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    12841 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    12901 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    12961 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    13021 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    13081 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    13141 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    13201 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    13261 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    13321 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    13381 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    13441 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    13501 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    13561 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    13621 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    13681 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    13741 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    13801 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    13861 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    13921 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    13981 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    14041 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    14101 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    14161 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    14221 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    14281 ggcgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    14341 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    14401 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    14461 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttattttata
    14521 gtgtatttat tctcagagaa tatatattac tgaaataaaa aacacaaaga ataaccaaag
    14581 gaaattattt gaaacgtaaa aggtatatat ttttaatctg taaaatttaa aatcacctcc
    14641 tctggattga atcaacttgc tctatttaag gcgattgttt ttacattttc accacctgta
    14701 tcaggtggtg tttttttatt ggcggcaatg cctttatctt atttttatta cgttgtaaat
    14761 atggctattc ctaaatgaaa tttgatttgc tcagtttatt tgtgatgtca ggggagtgct
    14821 tgccatgaga gtcgtgatat tattattcat catt
//
LOCUS       DADLCQ010000100        14565 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000100 DADLCQ010000000
VERSION     DADLCQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.100"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..581
                     /locus_tag="JQG95_002941"
     CDS             <1..581
                     /locus_tag="JQG95_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889435.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDYFVVI"
     gene            complement(635..1357)
                     /locus_tag="JQG95_002942"
     CDS             complement(635..1357)
                     /locus_tag="JQG95_002942"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY5889436.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(1359..2530)
                     /locus_tag="JQG95_002943"
                     /pseudo
     CDS             complement(1359..2530)
                     /locus_tag="JQG95_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(2557..4173)
                     /locus_tag="JQG95_002944"
     CDS             complement(2557..4173)
                     /locus_tag="JQG95_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY5889437.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            complement(4259..5653)
                     /locus_tag="JQG95_002945"
     CDS             complement(4259..5653)
                     /locus_tag="JQG95_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY5889438.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="JQG95_002946"
     CDS             complement(5672..7549)
                     /gene="bglF"
                     /locus_tag="JQG95_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY5889439.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="JQG95_002947"
     CDS             complement(7683..8504)
                     /gene="bglG"
                     /locus_tag="JQG95_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY5889440.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="JQG95_002948"
     CDS             complement(8791..9516)
                     /gene="phoU"
                     /locus_tag="JQG95_002948"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY5889441.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="JQG95_002949"
     CDS             complement(9531..10304)
                     /gene="pstB"
                     /locus_tag="JQG95_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY5889442.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSCADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="JQG95_002950"
     CDS             complement(10396..11286)
                     /gene="pstA"
                     /locus_tag="JQG95_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY5889443.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="JQG95_002951"
     CDS             complement(11286..12245)
                     /gene="pstC"
                     /locus_tag="JQG95_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY5889444.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="JQG95_002952"
     CDS             complement(12332..13372)
                     /gene="pstS"
                     /locus_tag="JQG95_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY5889445.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(13620..>14565)
                     /locus_tag="JQG95_002953"
     CDS             complement(13620..>14565)
                     /locus_tag="JQG95_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5889446.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         3757 a         3718 c         3316 g         3774 t
ORIGIN      
        1 tcggggcgct tacattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
       61 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
      121 tttattgcgt tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg
      181 cagaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
      241 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
      301 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
      361 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
      421 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
      481 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
      541 gcaagctgag actgtggggt gattatttcg tagttattta aataatgaga acaggccgga
      601 gcgtaattca tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag
      661 cggctttatc agcgattacc atcagcgaag gatgcagctg caacacggaa gctggaacat
      721 cttcggtaac cggaccttgc agcacatttt tcagtgcctg cgctttacct gcgccactga
      781 caattatcag caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt
      841 caggcactaa cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc
      901 cctggatcgg gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac
      961 aaaaatgacc atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca
     1021 gtttctgatc atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga
     1081 tccccgcagg ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt
     1141 tgccgcgaaa gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac
     1201 ctttaaccag ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg
     1261 ccaggttaac acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc
     1321 tcatttcctg gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc
     1381 tgtacgaagg ctgcataggc aggccgccag acatccattt catggttcag gccgggatat
     1441 tcctggtaat caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc
     1501 ttgccggtta cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta
     1561 atagctgccg gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta
     1621 acaccactga atgtggccag ccaaagcttt ccagatgatt cattccggaa accagcgcct
     1681 ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc ggcatcttta cggacactaa
     1741 aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc gctttagcat
     1801 tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga ataatgccct
     1861 tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg tttaattttc ccttcagcca
     1921 gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt ccggtatcac
     1981 caaaaccgtg atagaaatag agcactggca aaggctcgcc catgccggtg tatcctggcg
     2041 gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa gttatggcga
     2101 tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc agaatcatac
     2161 tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg ctgcgtacac
     2221 catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg cgccacgacc
     2281 agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga acacccacca
     2341 ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg ttatcggcat
     2401 tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct ggcatatccg
     2461 ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa gcagcaattt
     2521 ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt cagcctgggc
     2581 accgacagca aactgggcgt ctttattgtc ttcgaaggtg aagccatcca gttcgttttc
     2641 cagggctttg atatacgtgg cgtagaaacg aatttccaga cgcgaggtca acatactggt
     2701 attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa atttattact
     2761 attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct ggttatattg
     2821 gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag agtgtgcgcc
     2881 tgtttcgtag ctataaatat tgttaccgaa ggagtaaaca atcgcgttag ccacaataaa
     2941 atgatcgccg atataggctt cgccctgcga ggtcagacga actgctgttc cgccggtgtg
     3001 atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat aacggccaaa
     3061 gttcctggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc cacctttatc
     3121 aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat aatacccgtt
     3181 attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac gaccactcac
     3241 cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa tagtatgggt
     3301 attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt cgcgaaccag
     3361 cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac ccgctgcagc
     3421 atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg caccgtgttt
     3481 acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg taacgtacat
     3541 atcggagaac tgcataaagt tctcgccacc ggcattatcg ccaaaccagc cggtagagta
     3601 ctgctgacca acgttaccat ctatcatcac aacggcatca acccgtttgc cgttttcgtt
     3661 gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac caaagcggcc
     3721 cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc aaccagaacg
     3781 gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga aatctttcag
     3841 agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat ttttgaccag
     3901 tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat acccttgatc
     3961 attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct cttcatcttt
     4021 atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact caaggcgttg
     4081 ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg ccattaataa
     4141 gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg caaagaaaga
     4201 attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc tttattggtt
     4261 atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg aagcttttct
     4321 tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa ataaagccgt
     4381 agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc caactggtgt
     4441 accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg tgatcgttta
     4501 aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg tcttttacgc
     4561 ctaatccatt ctcgacaata aataacggtt tttgataacg atcccaaagc gtatttaaca
     4621 gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat
     4681 tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa
     4741 cacaaccagt catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg
     4801 cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc
     4861 cgggatattg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca
     4921 tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca
     4981 tattgccgat tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg
     5041 caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc
     5101 ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg
     5161 ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga
     5221 ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg
     5281 ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa
     5341 acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg
     5401 caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt
     5461 aacggtaata aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt
     5521 ccatttttcc cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt
     5581 ccagccaggc accttccacc tgattggcag ctgttgcacc accccaaaga aatgtttctg
     5641 gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac agcggttcac
     5701 ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga
     5761 ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa
     5821 tcagccgatc gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc
     5881 cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact
     5941 caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac
     6001 gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg
     6061 tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc
     6121 cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct
     6181 gacgtttact ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa
     6241 tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct
     6301 gcatgaaggt gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa
     6361 tgatggtggc ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca
     6421 ggttgacgcc atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc
     6481 ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga
     6541 cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga
     6601 agttattgat acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc
     6661 agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac
     6721 cggcggcaat catttcgctt atccaggttg atagcggccc caccagcaga aaggtgacgg
     6781 gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc
     6841 acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa
     6901 ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat
     6961 ccgctttttg cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa
     7021 tcaccatggc agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta
     7081 tcgggaagaa cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt
     7141 gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg
     7201 ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta
     7261 gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca
     7321 cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac
     7381 caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt
     7441 tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt
     7501 tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc
     7561 taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa
     7621 gcaatttttt tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata
     7681 tttcagtgtt ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt
     7741 gaaattttac gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat
     7801 gcttgcgggt aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa
     7861 gcatgttcaa gaatacgcca agataaaaac ttcagatgtg taaccagtcg ctgataactc
     7921 aagctttctt cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc
     7981 attaactgcg tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga
     8041 tgcatggcaa taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata
     8101 atggttaacg cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac
     8161 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
     8221 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
     8281 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
     8341 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
     8401 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
     8461 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
     8521 ataaacaaaa aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga
     8581 atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa
     8641 ttaatttaat tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct
     8701 cgcagttatt aactttatgt aattttagga atttataaag ttatatataa caaatcccaa
     8761 taattaagtt attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag
     8821 tttatccagc tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat
     8881 aaactcacaa atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag
     8941 cgcggtaagt acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg
     9001 aacaatacct tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc
     9061 gtcaatgtcc atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg
     9121 gcccagcgac tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc
     9181 agtacggcag attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact
     9241 gatcaccata accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac
     9301 gcaggcttca tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg
     9361 cttcgccaga tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc
     9421 caccatgccg cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg
     9481 gccggaaata tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa
     9541 cgaccggtga tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg
     9601 ttgctgaact caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca
     9661 gcctgctgca tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc
     9721 agctcttcaa tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc
     9781 agcacttccg ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca
     9841 gagagagagt aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg
     9901 gtcaatgccc actgcacgcg ctcgtccatg tcggcacagg agagcttctc aaacagacga
     9961 acgccaaagg cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc
    10021 atgcccactt tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca
    10081 tcaagcagaa tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag
    10141 gtacgcagca gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt
    10201 ttggcgatat ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa
    10261 ttacgaacct gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca
    10321 tcgtacctga tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc
    10381 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    10441 cagcagcagg acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc
    10501 cgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    10561 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    10621 ggcaatacgg gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag
    10681 cgtaatcgcg gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag
    10741 gctgtccggc accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa
    10801 cagcgccagc gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat
    10861 ggtgtagaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    10921 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    10981 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    11041 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    11101 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    11161 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    11221 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    11281 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    11341 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    11401 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc
    11461 atgtacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    11521 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    11581 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    11641 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    11701 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    11761 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    11821 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    11881 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    11941 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    12001 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    12061 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    12121 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    12181 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca
    12241 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    12301 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    12361 taatattggt cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt
    12421 aatccaggtc gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca
    12481 cctctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    12541 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    12601 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    12661 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    12721 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    12781 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    12841 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    12901 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    12961 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    13021 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    13081 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    13141 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    13201 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    13261 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca
    13321 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    13381 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    13441 gacagttata tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    13501 ctgattttaa atgtgaagat gaaaatccca catgccatct ttttgtcatg atgcatttaa
    13561 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    13621 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    13681 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    13741 actgttgatt tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    13801 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    13861 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    13921 ttttcacact caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca
    13981 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta
    14041 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    14101 tgtgagtaat ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt
    14161 tcaggaataa tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta
    14221 gccccactgg atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac
    14281 gggttgttag catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata
    14341 gcaatcccaa catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca
    14401 gcataagtta atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg
    14461 ttaccagtat cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta
    14521 aatgtcgttc caaccttatt ttcgttcgcg gtaataagat atccc
//
LOCUS       DADLCQ010000101        14485 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000101 DADLCQ010000000
VERSION     DADLCQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.101"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            691..1713
                     /locus_tag="JQG95_002954"
     CDS             691..1713
                     /locus_tag="JQG95_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY5889447.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1713..2693
                     /locus_tag="JQG95_002955"
     CDS             1713..2693
                     /locus_tag="JQG95_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY5889448.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2690..3448
                     /locus_tag="JQG95_002956"
     CDS             2690..3448
                     /locus_tag="JQG95_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5889449.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3458..4267
                     /locus_tag="JQG95_002957"
     CDS             3458..4267
                     /locus_tag="JQG95_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY5889450.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4267..5121
                     /locus_tag="JQG95_002958"
     CDS             4267..5121
                     /locus_tag="JQG95_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY5889451.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5147..7101
                     /locus_tag="JQG95_002959"
                     /pseudo
     CDS             5147..7101
                     /locus_tag="JQG95_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(7151..7405)
                     /locus_tag="JQG95_002960"
     CDS             complement(7151..7405)
                     /locus_tag="JQG95_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY5889452.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7606..8340
                     /locus_tag="JQG95_002961"
     CDS             7606..8340
                     /locus_tag="JQG95_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY5889453.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8342..8953)
                     /gene="emtA"
                     /locus_tag="JQG95_002962"
     CDS             complement(8342..8953)
                     /gene="emtA"
                     /locus_tag="JQG95_002962"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY5889454.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9053..9967
                     /gene="ldcA"
                     /locus_tag="JQG95_002963"
     CDS             9053..9967
                     /gene="ldcA"
                     /locus_tag="JQG95_002963"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY5889455.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10062..11798
                     /locus_tag="JQG95_002964"
     CDS             10062..11798
                     /locus_tag="JQG95_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY5889456.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            12409..13823
                     /locus_tag="JQG95_002965"
                     /pseudo
     CDS             12409..13823
                     /locus_tag="JQG95_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13991..>14485)
                     /gene="alr"
                     /locus_tag="JQG95_002966"
     CDS             complement(13991..>14485)
                     /gene="alr"
                     /locus_tag="JQG95_002966"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY5889457.1"
                     /translation="AATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLS
                     SEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTM
                     TVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVP
                     VVTV"
BASE COUNT         3532 a         3764 c         3610 g         3579 t
ORIGIN      
        1 gagaatgtta tcattgctac accctgtgct taataactgc aactctccgc cgttcagcga
       61 tgctttgata attctgttcc catagatatc gttatcaaaa taaagcgtaa atatcatatt
      121 tccgtaactg ttatcatctc tttttttgca cgtccgaata aacgcattca gtaaattact
      181 ctctctaaga aaataaatat cttcattgtt gttaatgaca ttaataacgt cgttcttaaa
      241 atcctcaaag tcttgttgcg gtaacttctg gttacgctgc ttactatcag caagcggtga
      301 atccggacga caacaacata ggttttgtat aacccccagc attcttttca cctccgttaa
      361 cagtatattc acgacactga aacgagagaa tgctgataat tttctcggcg agatatcaaa
      421 aatcgctcat tataaaacaa gtaacttcaa cgtcgcgtaa ccgtggacga taaaaaacaa
      481 taatgtcatt catatttaaa tgatttagcc ttaaatgatt taaataatat tagccacctg
      541 ataaatacat ttttaatgat agagagggtc aacaggcttg cggaagtcgt cacatcttca
      601 tgttttcaat agctaaactt tgatattgaa catgattttt atcgatatat atgaaagtat
      661 atatcgattt tacttttcag gatgttctcc atgcccataa ccagacggac gtttgcgcag
      721 gcacttgcat ctaccctgct cctgcagtct ctaccgtcct tttcacaaac agttaatcgt
      781 tttgcttcgc aatctcttcc cgaagcccaa aacattacac gcatcgtcag cgcaggcgcg
      841 cccgccgatt tgctcttgct ggctgtcgcg ccagaaaaaa tggtcggttt ttcctctttt
      901 gattttgccc gtcaggcatt aattccattg ccagagcaca ttcgccagtt acccaggctg
      961 ggacgactcg ccgggcgcgc cagcacacta tcgctggaag ggcttatggc gttacatccc
     1021 gatttggtcg ttgattgcgg caatacggat gaaaccttga tctcccaggc acggcaggtt
     1081 agcgaacaga cacaaatccc ctggttattg cttaacggaa aactggcgca atcagcagaa
     1141 cagttaacaa cgcttggcaa aacgttaggc gaagagcacc gcgccgcaga acaagccaat
     1201 ctcgccagcc acttcgttgg tgaagctcag gcattcgcca cctcacccgc cgctaacctc
     1261 cgcttttatg ctgcgcgcgg tccccgaggg ctggaaacgg gcttacaggg ttcgttgcat
     1321 accgaggcgg cggaattatt aggtttgcac aacgtcgcgc aaatagccga tcgccacggc
     1381 ctgacacagg tttccatgga aaatctcctg cgctggcagc cggatattat tctggttcag
     1441 gaggccgtta ctgcagattt tattcgtcgt gatcctctct ggcagggcgt gaaagccgtt
     1501 gcggaacaac gcatcttatt tttaagtggc ttgccctttg gctggctcga tgccccgccg
     1561 ggaatcaacc gtcttctggg attacgcaga cttcacgcct ggctggatcc cgccatcaat
     1621 cgccagttta aaagtgacat gcagcattac gcccaactgt tctggcattg ttcactcagt
     1681 gatgccgact atcaaaaatt ggtggcgagc taatgagaat cgtaaacgga tgtattttgc
     1741 tgctggcagc tatcagtatc acttttgccg ccgtaagtgg ggcttatcat ctcgatatgc
     1801 agcaactgct ggcgctaatt cttcgtcaag aaaatgagtc tgtgcaggaa caaattgtct
     1861 tctggcaaat ccgcgtgccg cgtattcttg ctgcgctgtt actgggcgca gcgctggcag
     1921 gagccggaac cacctatcag ggaatgttgc gtaatccctt agtttcaccg gatattttag
     1981 gcgtctccgc cggtgccgga cttggtgcct gcgcggcaat cctgtgggga ttacccattg
     2041 tttacatcca gctgtacgca ttttgcggcg ggctgatggt ggtcgcgggc gtctggttga
     2101 ttacgcgtcg ggtgacccgc cacgatccga tcttaaccct ggtgctggta ggcattgcgt
     2161 taggaacgtt gtgcggagcg gggatatcgc taattaaaac gctggccgat ccttacaccc
     2221 agttaccgtc aattaccttc tggttgctgg ggggactgtc caccgtgacc ttacgcgatc
     2281 tctgctatgc tgcaccgatc attctcatag gctccttgcc cctgtttttt ctccgctggc
     2341 gcatgaattt actcacgctt tccgacgacg aagcccgttc gctgggactg aacgtgacgc
     2401 gtctgcgctt tggattgatt gtctgcgcca cactaatcac cgccagcacg gtagcaatag
     2461 cgggcataat tggttggatc ggtctggtcg ttccgcacat tgcccgcttg ttgaccggtc
     2521 ataaccatca gcagctgttg cctatggcaa tgtgtactgg tgctattctg ctcctgctta
     2581 ccgatacgct ggcgcgcagc attggaacaa cagaaatccc acttggcatt ctgaccgctt
     2641 ttgttggcgc accgtttttc ctgcttttgt tgttacgcgg aggtcgccag tgactttgct
     2701 tgccgttcgt catgcgtctc tgggttactc ccgccatcca gtgctacgcg acgtgtcctt
     2761 caccctgtcg caaggaacaa tttgttgtct gcttggcgcg aatggttgcg gcaaaaccac
     2821 actcatgcgc tccattcttg gcgttatacc gttattaaaa ggcgagatcc tactggatag
     2881 cctctctgtg cagacactca gccatcgcca gcgcgcacag gctattgcct gggtgccgca
     2941 ggcgcacgac ggtatttttg ctttcagtgt gctggatatg gtgttgatgg gcctggcccc
     3001 caccataggc gctttttccg ttcccggtaa gcaagaacgc ctgaaagcca cggagcaact
     3061 ggaaaagctg ggcattttgc atctggcgac acgacgctgg aatacgctta gcggcggtga
     3121 acgtcagctg gctttaatcg cccgtgcact cgtccaacaa ccgcgcttgt tgttactgga
     3181 tgagcccgcc tccagcctcg atttcggtca tcagatccaa cttctggaca cgctggcgca
     3241 gctcaaaaat aacggcatga caatgttaat gtcgactcac catccgctcc atgccaacgc
     3301 cattgccgac agcattatcc aagtagagcc agatggacga gtaacacaag gcttaccgac
     3361 cgaacagtta acaacgaaca aactggcggc cctgtatcgg gtttctgccg accaaattca
     3421 tcatcacctc tctgcaatta gccgctaagg aacaatcatg ctcattgatg atattgattt
     3481 tgcagacctt tatctgcagc aactcaaact ggctcaccgc acagaaaaaa cgcccgatca
     3541 ctgggatcaa cgggcggaga aaatggcgga aaattgcgcc tcgccaacgg acagctacct
     3601 gcaacaactg atcgctaaaa ttgacctgca aggcgctcag accttattcg atatgggctg
     3661 cggaccaggc accgtatcgc tggcgctggc tgacaaactc actacggttt atggcgttga
     3721 ttacagtcag gggatgctga acgttgccgc ccgccgcgcc gccgcactga aggctgacaa
     3781 tgtccactgg atccagcgcg cctgggaaga ggactggagt gacctgccac gctgcgatat
     3841 tgccgtggct tcccgctcca ctctggtggc tgatatgcgc catgcaatga gcaaactgaa
     3901 taatcaggca cggcttcgcg tctatacaac tcatctggtc agcacctcgt tcgtctctcc
     3961 agccattcag cgggcggctg gtcgggaagt gatcgaatta cccaactata tctttgcgct
     4021 caacgtcctg tatcagatgg gaatttatgc ccacgttgat tttatccgtg gacaaaattg
     4081 ccaacaggat aacagcacct gggaacgttt tgagcagaac gtcagctgga gtcttggcgc
     4141 gcttaacgac gatgagcgtg agcggttata tcactggtat caacaacaag atgctcgtgc
     4201 cctcgcccct gccagccgcg actgggcgct catctggtgg gatagcgtac cgcaggaggc
     4261 actgtgatga ttttcctttc tcaggcacaa atcgatgcgt tactgctgga agatatccag
     4321 ggaggtgacc tgaccacgcg agcattaaat attggacacc agcctggcta tatagcgttt
     4381 tttcaccgtc agggcggctg cgtcagcggc gtttctgtcg cgtgtaagat gttaactacg
     4441 ctgggattaa ctattgatga cgcggtcagc gacggttcac aagcgaacgc gggtcaacgg
     4501 ctaatccgtg cgcagggtaa tgccgcagca cttcatcagg gatggaaggc agtccagaat
     4561 gtgctggagt ggagttgcgg tgtttctgat tatctcgctc aaatgctggc attgcttcgt
     4621 gaacgttatc ctgacggtaa tatcgcctgc acccgaaaag caattccggg cacccgctta
     4681 ctggcttcgc aggcaattct ggctgccgga ggactaattc atcgtgccgg atgtgcggaa
     4741 accatattat tgtttgccaa ccaccgccat tttcttcatg acaatcaggg ctggtcaggc
     4801 gcaatcgctc agttacgtcg ccacgcaccc gagaagaaaa ttgttgttga agccgacacg
     4861 ccgaaagagg ctatcgccgc gttacgcgca caaccagacg tacttcagct cgacaaattt
     4921 agtccgcaac aggcaactga aattgcgcaa atagcgccgt cgctggctcc tcactgcacg
     4981 ctggctctga ccggcggaat taatctgacg acactcaaaa attacctcga ctgcggcatt
     5041 cgccttttta ttacctccgc gccttattac gcggcaccag ctgacattaa agtcagtctg
     5101 caacccacag cctctattta attctttgtt aaacggaatt tattctatga gactcaaaaa
     5161 acgttatctc tgcacagcat tatcacttgc ctttacccag caggccgtag cggctcagga
     5221 gagcgacacg ctgaccgtat ggtccagtcc ggtatcatcg acgacgacca ccgttctcga
     5281 tcaacccacc atgaaggcct tggataaaca gaatgtcgct caggcattaa gtgtcgtccc
     5341 cggcgtggtg ctgcaaaagt caggcagccg caacgaagaa caggtaaaag ttcgtggctt
     5401 tgatagtcgg caggtgccgg tctatttcga cggtgtgccc atttatgtcc cctatgacgg
     5461 caacctcgat ctggcgcgga ttctgaccaa caatctgggg gcagtcgaag tttccaaagg
     5521 gtattcgtcg ctgcttcatg ggcctaatca gatgggcgga gccattaata tcaccaccct
     5581 gaagaagcaa gtctgggata tcgccaggga tggagccgta gtcaggacaa tgcctacgat
     5641 atgcatgctt catttgccgc cagcagcgat cttgggtatt tgcaagtcag cggtagccag
     5701 ctaaagcagg attttctcgg cctgccgcat ggtgtaaata atgatattgc aggcaaacac
     5761 ggcaagatga ttaattcatc ggctgatgat aaacgcggca ttgtgaagct cggttttaca
     5821 ccgcgcgaaa acgatgaata cacgctgact tacattaagc aggatggtga aaaagataac
     5881 ccgccataca gcggaaattg tggtcaaaaa tcacgctact ggcagtggcc agagtatgac
     5941 aaagaaagtt tttattatca gggagcgacc caactaaacg accgttttac cctgaaaagt
     6001 cggctgtatc gcgacacctt tgaaaatacg ctgatgatgt acaactcgct ggctgatttg
     6061 aaaaataaaa aaggcagcta cagccattat tccgattaca gcgacggtgc cggattacaa
     6121 ctggcagccg atgtgcgtga aaacgatctg ctgtcgtttg ccgttaactg gaaagatgac
     6181 gtacaccggg aaaaaggtgc gccgcacgcc gcttacgatc gctatgaaga tcgtacctgg
     6241 tcgctcgcca gtgaatatca atgggctgct gctgataatg tcgatgtcgt ggcgggaatc
     6301 agctatgact ggcgcgatag cgtagaagcg aaaaaacacg agaaagatgg cagcatcacc
     6361 cattatgacg acaacaatca gtcagctttt aactggcagg tgatggggaa ataccacttt
     6421 gccaatgaag acactctggc gctttcgtac tatgaccgca cacgctttcc gacgctgaaa
     6481 gaacgctata ccacatccaa acctgcgtat aaccagatag cgattgttaa cccgcagctc
     6541 aagccggaac gcgctcgcgg ggtggattta acctggaatg gtgccttcac gcgcgactgg
     6601 ggatttgagg tcagcgttta ctataaccgg gtgagtgatg ccatcctctc gcacaatatc
     6661 aatgccgata ccattcaaaa tcagaacagc ggcacggtgg attacagcgg tctggatgcc
     6721 ggtattaagg ggaaaatcag caatatactg gatgtaggat tgagctacgc tctgatccac
     6781 gctgacgcca aacgcaaaga catcggcaag ataaccgatc ttccaacgca gacaatgacc
     6841 gcatggatga cactcaaacc atgggagccg ttaagcgtaa cgctgtcgga agaggcgcgt
     6901 tcctccagct acagcaacag tgacggttca caaaaagccg ccggttttgc ggtgacccac
     6961 attcgagccg attacacctt aggtcatggc ttcagcgtca atgcgtcggt caataacctg
     7021 tttgataccc aatatgccta cagtgaaggg tttattgaag aaggtcgcaa tttctgggca
     7081 ggtgtcgagt atacgttctg atgtgaaagc ggatagcggc atctgtatcg ctatccgcct
     7141 tatgaaaatc ttattctctt cgcaggaggc ggaatacgcc cagcaccagg atagcgccca
     7201 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgtca atgccaaaca
     7261 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
     7321 atccaccacc atcacgcccc ggcatgatta gcttgccgat aatgccggct atcaggccaa
     7381 aaataatcca ggcaataatt cccatgttca ggccctcgtt tcgatgaatc ggtcgcaaga
     7441 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtttgtcg
     7501 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaacta
     7561 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
     7621 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
     7681 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
     7741 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgccgtg
     7801 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
     7861 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttattac cgtaccgcct
     7921 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
     7981 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cttgtatgat
     8041 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
     8101 ggtatgcgct tcgctcaaat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
     8161 gcccagttaa tctccaccag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     8221 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     8281 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     8341 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     8401 gatgatttcg cgctacgtga tcgaggaact cgtcagcatc caaatcgttg attttgctga
     8461 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     8521 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     8581 ccagaatatt caggtaagcc gcccccattg aaatattacg ctctggattt ttcagctcgc
     8641 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     8701 cttttaactg catcaaacca atggcattcg atttactcac cgcgttcgga ttaccacccg
     8761 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     8821 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     8881 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     8941 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     9001 cacctaagcg gcatgatata catttcgttt ctgttgtcag caaggaattg ccatgtctct
     9061 gtttcactta attgccccat cgggttacag cattaaacag cacgccgcgc tgcgtggtat
     9121 ccagcgcctt accgacgcgg ggcatcaagt caataacgtc gaggttattg cccgtcgttg
     9181 tgagcgtttt gcgggtacgg aaacggagcg tctggaagat ctcaattccc ttgccagact
     9241 gactaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     9301 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tactcatttg
     9361 cggacatagc gattttaccg ccattcagtg cggtcttctg gcgcaaggca atgtcatcac
     9421 ctttagcggc cccatgctgg tagcaaactt tggtgcggat gaactgaacg cctttaccga
     9481 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     9541 cccgacatgt cagaccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     9601 gattggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     9661 cgtgcaccct ttccgcgtcg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     9721 acgtcagaag gcgattattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     9781 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     9841 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     9901 cattctgaat aacacccggg aaggcgctca gttgacgatt agcggtcatc ctgttcttaa
     9961 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
    10021 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
    10081 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
    10141 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcgggag
    10201 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
    10261 tggcgctggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
    10321 tggcgttagg accggcgctg tcgctggcga cgctgggcgt acttatcacc tctggtttaa
    10381 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
    10441 ctatcgtcgg ctccaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
    10501 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
    10561 ttctgacgat taccctgatt gcaatgatcc agcagcatga aagcagtgtc agttggatgt
    10621 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggtctt ggcggtggtt
    10681 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
    10741 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
    10801 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
    10861 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
    10921 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
    10981 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cctttccgcg
    11041 gtttcaatct gcgtgagcgc gtgtttatca gctgggtggg attacgcggc gcggtgccaa
    11101 tcatcctagc cgtgttcccg atgatggcgg ggctggagaa tgcgcgactg ttctttaatg
    11161 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
    11221 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc ggtgtcacgc gttggcctgg
    11281 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgct gataaatggt
    11341 gtgtaggtgc ggcactgcgc gatttgcata tgccaaaaga gacgcgtatt gcagccctgt
    11401 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtat
    11461 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
    11521 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
    11581 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataaacagc
    11641 aaacgctggg tgaaattgtc cagcagttgc taggcgcggc accggttgtc ggtgaccagg
    11701 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
    11761 ttggcgttcg ggtggctgaa gaggaagctg aatcttaaat ttaatgcagc atctatcgcc
    11821 ggatgcgatg ctttactgaa cgcagcctga gcctgtaata aacttgtcat ttcctgattt
    11881 tgatatgctc atcccaacat cagaagaagg ttaaattatg gatgacaaac cattgcaggc
    11941 tctggctaat gaactcaaaa caccggctca agaaagtcag cgtaagggct ttttccgact
    12001 gtaacaggta ataaaaacca gccccgcatt accggtcaac catcaggggc tcctgcctga
    12061 catcgcctga acatattccc ccacgcaacc tgccgcactg acatagccca cacaataggc
    12121 gtttcatccg cctgtagcac cggatgcatg agcgatttct tatagttatt cctttgctta
    12181 ataacgatta tttccgggtc gttcactctc gtattttctt taatacgtca gagctaccta
    12241 ggccgtaata cagcatgtac cagacccgac acggcatttc tgaccaggga aaaagtcaac
    12301 gatgagtagt gatatcagcg caaatgacgt cctgtggctc ctttgcggtg accacattca
    12361 cgtctccagc gctgccagag gatatttatt tcaaggaaac gtagcattat ggctagcttt
    12421 aaagacatct gcccattcgc ccccgccatt cccccatctt ccgttaaccc aggcgtacga
    12481 cgcccgccag ggcggctctt ctcaagaatc aaattttgca gaacatgaag tatctcagcc
    12541 cgcaaaaatt cagctggggt gatgccccct ggcagatcat tgacctcagt atcgcgggca
    12601 aagtgaacat acaggtggac aacaatacca tcatcacgtt ggggactcgt ctgaatcagc
    12661 aacataacga gttcatgatg gtcgccaaat ggtgcgaatg ggctatacag caggatggtt
    12721 tgcaagaaaa cctgcaaaaa aacctgtacg aaatcctgga agaaaaccag caaaacaaac
    12781 agtcagaaat tccccaagaa gacctgaaag aaagtctgga agaaatcaag gaaaatatcc
    12841 tggaagaaaa cctgccagca agccggattg aaaaccgggc agaagcattg cgcagaatga
    12901 aggaatgtct cattacaaga cggagtatgc tcaatcttag caacctggga ttgacttcac
    12961 tccctgagaa tttgcctcca catctgattg aattttactg cagtaaaaac gtgttgaccg
    13021 cgttaccgaa ggtgatgcca aagtggctgc tggtgcttga ctgtacagat aacgtcttga
    13081 tcttgttacc gaaggtgcag ccctcaaaac tgatggtact gaagtgctat gaaaacagta
    13141 ttatctggct gcctgagctg tcgactaacc tgagagtgat taactgttct gaaaacttct
    13201 tgcaattttt accgccgtcg atgccccagt acctgtataa actatcctgt gccggcaaca
    13261 atattaactc aatacctgat gagatgctgg agaacttgac tcgcctgaaa gtatttgact
    13321 gttctagtaa cgatttgatc tcttcaccac ggctgccgcc caaactaata atttactact
    13381 gtggagaaaa caagtttaaa acggtacagg tgccgcagcc ccagagcctg aaggtgtttg
    13441 actgtaatgg taacccgtgg gacaaagaca atttaccgac gctgctcaaa gccgtcgagg
    13501 gcctgaaaaa acagcagggc ctgaaggacc ttttggactt tttgcacaag gaaggctagg
    13561 ttgacctgga aggactcgag gagctggagg accttatgga tctggagttc ctggatgacc
    13621 cggaactcct ggagcgcgtg aaggtacagg aggacctgga gctcctggat caacagttgg
    13681 gcctgttgag tctggaaaga caacaggact cgcagcctgt taatcaacaa tctgaacatg
    13741 aacccgaatc tgcatcaagg gtgaagcgtg atttatctga agtcgactcc gagtcaacaa
    13801 tgaagcgtaa gcgttttatg tagaacgcct gtgcggcgag ttcaggttcc ggggaaatag
    13861 tgtccattct tttaatggac actatcatgg gcaatggctc tgtcacatta acaactacag
    13921 ttgctgacca gccggatgga tgattttttc gcaaccagaa gacgctgcct ccgatccggc
    13981 ttacaacaag ttacactgtc acaaccggga cgcgtaacgc cagcgcgcac atcaactcat
    14041 agcccaccgt tccggcagcg gcggcgacat catcaatttt gatctccttg ccccacagct
    14101 caaccggcgt accaatcccc gcctgcgggc aaggcgttaa atcaaccgcc agcatatcca
    14161 tcgagacggt ccccaccgtc atggtgcgca cgccgtccac taaaacagga gtaccggtag
    14221 gcgcatggcg cggataaccg tcggcgtatc ctgcggcgac aatgccgatt cgctgttcat
    14281 cgcgcgcagt atagcgaccg ccgtagccca cacgctcgcc cgcttttagc gtctggacac
    14341 caataatctc actgcttagc gtcatcaccg gacgtaatcc ggtattggcg atatcacgcc
    14401 actgaccgga cggcgaagcg ccatacaaaa taatgccagg ccgaacccag tcaaaatgcg
    14461 cttccgggtg ccacagagtc gccgc
//
LOCUS       DADLCQ010000102        14481 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000102 DADLCQ010000000
VERSION     DADLCQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.102"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(115..381)
                     /locus_tag="JQG95_002967"
     CDS             complement(115..381)
                     /locus_tag="JQG95_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY5889458.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="JQG95_002968"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="JQG95_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY5889459.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="JQG95_002969"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="JQG95_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="JQG95_002970"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="JQG95_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY5889460.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="JQG95_002971"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="JQG95_002971"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY5889461.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="JQG95_002972"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="JQG95_002972"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY5889462.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="JQG95_002973"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="JQG95_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY5889463.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="JQG95_002974"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="JQG95_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="JQG95_002975"
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="JQG95_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY5889464.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(12643..14097)
                     /locus_tag="JQG95_002976"
     CDS             complement(12643..14097)
                     /locus_tag="JQG95_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY5889465.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="JQG95_002977"
     CDS             complement(14094..>14481)
                     /locus_tag="JQG95_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5889466.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3862 a         3652 c         3420 g         3547 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaactgc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaagatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatccgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tgcaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DADLCQ010000103        14401 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000103 DADLCQ010000000
VERSION     DADLCQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.103"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..561)
                     /gene="thiE"
                     /locus_tag="JQG95_002978"
     CDS             complement(<1..561)
                     /gene="thiE"
                     /locus_tag="JQG95_002978"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY5889467.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVS"
     gene            complement(561..2456)
                     /gene="thiC"
                     /locus_tag="JQG95_002979"
     CDS             complement(561..2456)
                     /gene="thiC"
                     /locus_tag="JQG95_002979"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY5889468.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     DMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(2537..2637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2690..3166)
                     /gene="rsd"
                     /locus_tag="JQG95_002980"
     CDS             complement(2690..3166)
                     /gene="rsd"
                     /locus_tag="JQG95_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY5889469.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            3261..4034
                     /gene="nudC"
                     /locus_tag="JQG95_002981"
     CDS             3261..4034
                     /gene="nudC"
                     /locus_tag="JQG95_002981"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY5889470.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            4074..5138
                     /gene="hemE"
                     /locus_tag="JQG95_002982"
     CDS             4074..5138
                     /gene="hemE"
                     /locus_tag="JQG95_002982"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY5889471.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            5295..5966
                     /gene="nfi"
                     /locus_tag="JQG95_002983"
     CDS             5295..5966
                     /gene="nfi"
                     /locus_tag="JQG95_002983"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY5889472.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            6009..6599
                     /locus_tag="JQG95_002984"
     CDS             6009..6599
                     /locus_tag="JQG95_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY5889473.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            6786..7058
                     /gene="hupA"
                     /locus_tag="JQG95_002985"
     CDS             6786..7058
                     /gene="hupA"
                     /locus_tag="JQG95_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY5889474.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            7071..7766
                     /locus_tag="JQG95_002986"
     CDS             7071..7766
                     /locus_tag="JQG95_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY5889475.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(7768..8193)
                     /gene="zraP"
                     /locus_tag="JQG95_002987"
     CDS             complement(7768..8193)
                     /gene="zraP"
                     /locus_tag="JQG95_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY5889476.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            8431..9807
                     /gene="zraS"
                     /locus_tag="JQG95_002988"
     CDS             8431..9807
                     /gene="zraS"
                     /locus_tag="JQG95_002988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY5889477.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            9804..11129
                     /gene="zraR"
                     /locus_tag="JQG95_002989"
     CDS             9804..11129
                     /gene="zraR"
                     /locus_tag="JQG95_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY5889478.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVMLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(11126..12415)
                     /gene="purD"
                     /locus_tag="JQG95_002990"
     CDS             complement(11126..12415)
                     /gene="purD"
                     /locus_tag="JQG95_002990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY5889479.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(12427..14016)
                     /gene="purH"
                     /locus_tag="JQG95_002991"
     CDS             complement(12427..14016)
                     /gene="purH"
                     /locus_tag="JQG95_002991"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY5889480.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         3244 a         3816 c         4014 g         3327 t
ORIGIN      
        1 gctgacgacg gcgatactgc cgacacccgt tgctatcacc gcaggcgcgc gtgccagact
       61 gataccgcca atcgccacgg tgggataatc cgccaggcgc tcaacatgcc gtgccaactg
      121 ttccagcccc tgcggtgcag aaggcatctg tttggtttgc gtcgggaaca catgtcccag
      181 cgcgatataa gaggggcgtg ctgccagcgc gacgtcgatt tccatatcgt catgtgtcga
      241 aacgcccagc cgcaggcctg ccgcgcggat ggcattgaga tcggtggctt gcaaatcttc
      301 ctgccccaaa tggacgccat acgcctgatg cttgatcgcg agccgccagt aatcgttgat
      361 aaacaatcgc gcgttatagc ggcggcccag cgcaattgcc gccacgacat cggcttccac
      421 ctcttcatcg cgccgatctt tgatgcgtag ctggagagta cgtacgcctg catccaacag
      481 acgttcgatc cactgtacgc tgtccaccac cgggtacagt cctaaacgaa aaggtacagg
      541 aggaaaatca ggctgataca tcacgcttcc tccttatgca ggtagatttc tccgcctctg
      601 gcacggaagt tctccgacat atccgccatt cccacttcaa tggtttgcgc ggcggcgtaa
      661 tcgcgtactt cctggctgat tttcatcgag cagaattttg gcccacacat ggagcaaaaa
      721 tgggcgactt tgccggactc ttggggcagg gtttcatcgt gataagcgcg ggcggtaaac
      781 gggtcgaggg ccagattaaa ctggtcttcc cagcgaaatt cgaagcgggc tttcgacatg
      841 gcgttatcgc gaatttgcgc gcccggatgc ccttttgcca ggtcagcagc gtgggcggca
      901 atcttataag tgataagccc ctgcttaaca tcttctttat tgggcagacc cagatgctct
      961 tttggcgtta cgtaacagag catcgcgcag ccaaaccagc caatcatcgc cgcaccaatc
     1021 cccgacgtga agtggtcata gcccggtgca atatcggtag ttagcggccc cagagtgtaa
     1081 aacggcgctt cgtggcagtg ctctaactcc tcggtcatat tgcggcggat catctgcatc
     1141 ggcacatggc ctgggccttc aatcatcacc tgtacgtcat attcccaggc gattttggtc
     1201 agttcgccca gcgtatgcaa ttcggcaaac tgcgcttcat cgttggcgtc ctgaatagaa
     1261 ccggggcgca gaccgtcgcc cagcgacagc gaaacgtcat aagcggcaca gatttcacaa
     1321 atttcgcgga agtgttgata gaggaaattt tcctgatgat gggagaggca ccatttcgcc
     1381 ataatcgaac cgccgcgaga gacgataccg gtcaggcgtt tcgcggtcat cggcacatag
     1441 cgcagcagta cgcccgcatg gatagtgaag taatccacac cttgctcggc ctgttccagc
     1501 agcgtgtcgc ggaacgcttc ccaggtaaga tcttcggcga tcccgttaac cttctccagc
     1561 gcctggtaga tcggcactgt accgatcggc accggactgt tacgcaaaat ccactcgcgg
     1621 gtttcgtgaa tatagcgacc ggtggagaga tccatcaccg tatccgctcc ccagcgcgtg
     1681 gaccatacca gcttttccac ttcttcttcg atggaagagg tgaccgccga gttgccgata
     1741 ttggcgttaa cttttaccag gaagttacgg ccaataatca tcggctccga ttccggatga
     1801 ttaatgttgg ccgggataat cgcacgtccg gcagcaactt catcacggac aaattccgca
     1861 gtgatatttt ccggcagatg tgcgccaaag ctcattcccg gatgctggtg gcgtaaaacc
     1921 tcgctacgga tgcgctcgcg gcccatattc tcgcggatgg cgatgaattc catgtccggc
     1981 gtgatgatgc cctggcgggc gtagtgcagt tgggtgacac ggcgtcctgt tttggcgcgt
     2041 tttggcgtta ataagccgct aaaacgtagc tcgtcgagac catcatctgc cagccgcgtt
     2101 ctggtgtaat cggaactgcg cacggtaagt tcttcggtat cggcgcgcgc atcgatccac
     2161 ggctggcgta gttttgccag cccttgctgc acgttaatgg cgatctgcgg atcaccatac
     2221 gggccggagg tgtcgtagac cggaatcgct tcgttttctt cgtactgcgg ctgttctttg
     2281 ctaccgccaa ttagcgtcgg gctaagctgg atctcacgca tcggcacgcg cacgccgggg
     2341 tgtgtgccag tgatataaat gcgttttgag ttgggaaagg cggtgccttc cagggtgtcg
     2401 ataaaatgtt gggcccgggc gcgttgttcg cggcgggtca gttttgttgc agacatagct
     2461 cattccaaaa agttaaggac gtggcttgtc agacgacgga tgaagcaaga gacgatcgcc
     2521 gcaaaggcga tgcgatagca gattaactct tgttcccttc gcaggtatta gcctgatcag
     2581 gttccgcgga tcccgaataa acggtctcag ccagttaagg cactccgaca agaaattagc
     2641 ccccacaaac ggggcgttga atgtaaatta cgcgttaaca gcgcagaact caagcaggat
     2701 gtttgacgcg ggcggcgtca agcaccagca gaatcagctt atcttccagc acaaagcgcg
     2761 cttccagcgc ctcgccgatg tcagataaaa cctgttgaaa ctcaaggtaa ttatcatgat
     2821 cgatggcggt ttccaggctg gaatcgtagt aatccataat ctgttgggta ttggcttcga
     2881 gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt gccttccagc ttatgaagaa
     2941 tgcgctcata aatactgaaa tgtccggcag acaagtaatc gaccaggctc tgacaaaaat
     3001 catcaagggc tttttcgttt agcctcatgt acgattcttt gccaggctta atgccaacca
     3061 gattgtagta agccacgagc agatgcttac gtacatgtag ccagcgatca accagtttgt
     3121 tacttcctct gacgcgctcc gtcaggttat cgagctggtt aagcatgatt gactccgcaa
     3181 gtttgtattc aaaaactgct cagtgagaaa tgtaaaaacc atgttaaaca tgccagtgat
     3241 gcaaaggtag tgcaagagct atggatcgta taattgaaaa attagatcac ggctggtggg
     3301 tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga attgccatat ggcgaagcgg
     3361 caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg cgaatggcag ggggaacctg
     3421 tttggttaat acaacagcag cggcgtcacg atatggggtc ggtacgtcag gtcattgatc
     3481 tcgatgttgg gctgtttcaa ctggccggac gtggcgtgca actggcggag ttttaccgat
     3541 cgcataaata ctgtggttac tgcgggcatg aaatgtatcc gagcaaaacc gaatgggcga
     3601 tgctgtgtag ccattgccgt gagcgttact acccgcaaat cgccccctgc attattgttg
     3661 ccatccgtcg cgatgattcg ctcctcctcg cccagcatac ccgccatcgt aacggtgtcc
     3721 atacagtact tgccggattc gtcgaagtgg gcgaaaccct cgagcaggca gtcgcgcggg
     3781 aagtgatgga agagagcgga attaaagtta aaaacttgcg ttacgtgact tctcagccgt
     3841 ggccgtttcc tcagtcttta atgaccgcgt ttatggcgga atatgacagc ggcgacatcg
     3901 tgatcgaccc gaaagaattg ctcgaggcga actggtatcg ctatgacgat ttgccgttac
     3961 tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga tactgtggcg atgtgtcggg
     4021 cagagtatga gtgatgatac actgaccgcc tgacgcacta aggaacagcc aaaatgaccg
     4081 aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca gcccgttgat gtcactccag
     4141 tatggatgat gcgccaggcg ggtcgctatc taccggaata taaagccacg cgcgcccagg
     4201 cgggcgattt tatgtcgctg tgcaaaaacg ccgagctggc gtgcgaagtg actttgcaac
     4261 cgctgcgtcg ctacccgctg gatgcggcga tcctcttttc cgatatcctc accgtgccgg
     4321 acgcgatggg gttagggctc tattttgaag ccggagaagg tccgcgtttt acctcgccag
     4381 tcacctgcaa agccgacgtc gataaactgc caattccgga cccggaagat gagctgggtt
     4441 acgtgatgaa cgcggtgcgt accattcgtc acgaactgaa aggcgaagtg ccgctgattg
     4501 gtttttccgg cagcccgtgg acgctggcaa cctacatggt ggaaggcggc agcagcaaag
     4561 ccttcaccgt gatcaaaaaa atgatgtatg ccgatccgca ggcgctgcac gctctgctcg
     4621 ataaactggc gaaaagcgtc actttgtatc tgaatgcgca gattaaagcc ggtgctcagg
     4681 cagtgatgat tttcgacacc tggggcggcg tgcttaccgg gcgcgattat caacagttct
     4741 cgctctatta catgcataaa attgttgatg gtttactgcg tgaaaacgac ggtcgccgcg
     4801 tgccagtcac gctgtttacc aaaggcggcg gacagtggct ggaagccatg gctgaaaccg
     4861 gttgcgatgc gctgggcctc gactggacaa cggacatcgc cgatgcgcgc cgccgtgtgg
     4921 gcaataaagt cgcgttgcag ggtaatatgg atccgtcgat gctgtacgcg ccgcctgccc
     4981 gcattgaaga agaagtagcg actatacttg caggtttcgg tcacggcgaa ggtcatgtct
     5041 ttaaccttgg tcacggcatt catcaggatg tgccgccaga acatgctggc gtgttcgtgg
     5101 aggcagtgca tcgactgtct gaacagtatc accgctaaga gcctgatcca tcaggctatt
     5161 ttatttgcca ttttgaggcc gggcagtgct caaaatcctc acgtactacg tgtacgctcc
     5221 ggtttttccg cgctgtccgg cctcaaactg ccttcaacaa ttacgcctgt tgaaccaggc
     5281 tctaaggagc gattatggat ctcgcgtcat tacgcgctca acaaatcgaa ctggcttctt
     5341 ctgtgatctg cgaggatcga ctcgataaag atccaccgga tctgatcgcc ggagccgatg
     5401 tcgggtttga gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatatccct
     5461 cgcttgagct ggtggagtat aaagttgccc gcatcgccac caccatgcct tacattccag
     5521 gttttctttc cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa
     5581 agccggattt agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtcg
     5641 ccagccattt tggcttattg gtggatgtgc cgaccattgg cgtggcgaaa aaacggctct
     5701 gcggtaaatt cgaaccgctc tccagcaaac cgggcgcgct ggccccactg atggataaag
     5761 gcgagcagct ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta
     5821 ccggccatcg ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct
     5881 atcgtctgcc ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg
     5941 tgcgctatac agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag
     6001 aactcaacat gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa agctggcagc
     6061 atgtcacttt catggcttgc ttatgcgaac gcatgtaccc caattacgcc atgttctgcc
     6121 agcaaaccgg ttttggtgat gggcaaattt accgtcgtat tctcgatctc atctgggaaa
     6181 cgctgaccgt taaagacgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag
     6241 cgattccttc agccgacgat ttcgatctgt acggcgttta tccggcaatc gatgcctgcg
     6301 tggcgttaag tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa cacgcggtgg
     6361 aagtgagtaa gacctccatc acgaccgttg cgatgctgga aatgactcag gctggtcgcg
     6421 aaatgggcga tgaagagctt aaagaaaacc cagctgtaga gcaagaatgg gacattcagt
     6481 gggaaatatt ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc
     6541 ttagggcaga cctgcgtgag tcgggtgaga gcaatattgg tataattttt cagcaataag
     6601 accagaaaac gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc
     6661 gtctggtcta catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt
     6721 gctattctgg catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat
     6781 aacttatgaa caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa
     6841 cccaggctaa agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag
     6901 gcgatgctgt acaactggtt ggtttcggta ccttcaaagt gaaccatcgc gctgagcgta
     6961 ctggccgcaa cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat
     7021 ttgtttctgg caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt
     7081 ttaacgaagg ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggcag
     7141 gtgcgctgtt tctcactgcc tgtagccaca actcttcact tcctcccttt accgccagtg
     7201 gatttgctga agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc
     7261 atctgcttgc cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct
     7321 ggcagggcga taacctcacg ctcatcaata tcaatgttta cagcaaaccg ccggtgaaca
     7381 ttcgcgcgcg ttttgacgat cgcggcgatc tgagctttat gcaacgtgaa tccgatgggg
     7441 aaaagcagca gctttctaac gaccaaatcg atttataccg ttatcgtgct gatcagatcc
     7501 gccagattag cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg
     7561 cgatggaaca gaccgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc
     7621 aggcgatagc gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat
     7681 ggctggaagc gcccgaaggt tcgcaattac tgttagtggc aaactctgat ttctgtcgct
     7741 ggcaacccaa cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc
     7801 accgcagccg ccgtagccca tgcccattcc ggctccgcgc ggaatacccg cttcagccat
     7861 cgcgatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct ccatctcttt
     7921 ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg cattgtattc
     7981 ataacgcttc gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa agtcattatg
     8041 gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt gctgccacat
     8101 accgtgtccg ccgtgagcaa atgcagatgt cgatcccatc gccattgctg aaagcgccat
     8161 cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg ttgtcttact
     8221 tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta atctggcatg
     8281 ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac tcattgaaac
     8341 ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg atttctgctg
     8401 tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc taaagactcc
     8461 ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt agggttgttt
     8521 gcggtgacgg tgattcgcga ttatgggcgc gagactgccg ccgccagaca aacgctgctg
     8581 gaaaaaggca gtgtacttat tcgcgctctt gaatccggct cgcgcgtcgg catggggatg
     8641 cgcatgcatc atgcgcagca gcaggcctta ctggaagaaa tggccgggca gcctggagta
     8701 cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa ctccggcatg
     8761 gtgggaaaac agctttattc ccctcaggaa atgcagcagt tacatccggg agatgaagaa
     8821 gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta tcgccagttt
     8881 caaccgatgt ttgctgctgg aatgtaccgg atgcgccata tgcagcagta tgccgcgaca
     8941 ccacaagcaa ttttcatcgc tttcgacgcc agtaatattg tgagtgccga agatcgtgag
     9001 cagagaaaca ccctgattat tctcttcgcc ctggcgacgg tcttgctggc aagcgtattg
     9061 tcattcttct ggtatcgccg ctatctgcgc tcgcgccagc ttctacaaga tgaaatgaag
     9121 cgcaaagaga agctggtggc gctggggcat cttgcggcag gcgttgccca cgaaatccgt
     9181 aacccacttt cctcgattaa aggactggcg aaatactttg ccgagcgcgc gcctgcaggg
     9241 ggagaagcgc atcaactggc gcaggtgatg gcgaaagagg ccgaccgttt aaaccgcgtg
     9301 gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc ggtggatctc
     9361 aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag ccgggagatc
     9421 cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc ggacaggctg
     9481 actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca gcatggcgtg
     9541 attagcgtga cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac cgacagcggt
     9601 aagggaattg cggcagatca gcttgaagcc atcttcactc cgtacttcac caccaaagcc
     9661 gaaggcaccg gattggggct ggcggtcgtg cataatattg ttgaacaaca cggtggtaca
     9721 attcaggtcg caagccagga gggaaaaggc tcaacgttca ccctctggct tccggtcaat
     9781 attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg gtggtggatg
     9841 atgacattag ccactgcact attttgcagg ctttactgcg cggctggggc tataacgtcg
     9901 cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt tttgatcttg
     9961 tgctttgcga tgtgcgaatg gcggagatgg acggcatcgc cacgctgaaa gagatcaaag
    10021 cgttaaaccc ggcaattccg gtgctgatta tgactgcgta ctccagcgtc gagacggcgg
    10081 tagaggcact gaaaactggg gcgcaggatt atctcatcaa gccgctggat ttcgataacc
    10141 tacaggcgac gctggaaaag gcgctcgcgc atacgcacag tattgatgct gaaacacctg
    10201 cggtgactgc cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa cacctgctca
    10261 gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtgct gatccacggc gattccggca
    10321 ccggtaaaga gctggtcgcc agggcgattc acgccagtag cgcacgtagc gaaaaaccgc
    10381 tggtaatgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa ttgttcggtc
    10441 acgaaaaagg ggcgtttact ggagccgata aacgccggga agggcgcttt gttgaggcgg
    10501 acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg caggtgcgtc
    10561 tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag actatctcgg
    10621 ttgatgtccg gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg aatgccgggc
    10681 gttttcgcca ggatctctac tatcgcctga atgtggtggc gattgaagta ccatcgctgc
    10741 ggcaacggcg ggaagatatc cctctgctgg ctggccattt tcttcagcgc tttgccgagc
    10801 gtaatcgcaa ggcggtaaaa ggctttacgc cacaggcgat ggatttgctg atccactacg
    10861 actggccggg aaatattcgt gaactggaaa acgcggtgga acgggcggtg gtgctgctga
    10921 ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgccagtacg ccgatcccgc
    10981 tggggcaaag tcaggatatt cagccgctgg tggaagtgga aaaagaggtg attctggcgg
    11041 cgctggagaa aacgggcggc aacaaaaccg aagccgcccg tcagttaggg atcacgcgca
    11101 aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatggcg cgccaaccga
    11161 tatctttccg gcagaagcaa tcatcccagt ggatatcggt cattaaggcg taggcgcgtt
    11221 tctgcgcttc tgccacggta tgacccagcg cggtgacgca cagtacgcgc ccgccgctgg
    11281 ttaccacctg ctcgtcatcc gccagttttg tgcccgcgtg gaacactttg ccgtcagcca
    11341 cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc ggatatccac
    11401 ccgcagccat caccacgctg agagaagcac gttcatccca ctctgacgtt ttctcgtcca
    11461 gtttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg cgcagcataa
    11521 tcggctgggt ttccggatcg ccaaagcggc agttaaattc aataaccttc ggattgccct
    11581 gtttgtcgat catcaggccc gcatagagaa aaccggtgta ggtgttgcct tccgccgcca
    11641 tgcctttcac ggttggccag atgatacgtt ccatggtgcg ctgatgaact tcatcggtta
    11701 ctaccggcgc gggggagtaa gcgcccatcc cgccggtgtt tggtccgtta tctttatcgc
    11761 ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg tccaccatca
    11821 cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga tgacccgcgt
    11881 cgccaaaagc gttgcccgcc agcatatcgt gaacagccgc ttccgcttct tccagcgtca
    11941 tcgccacgat aacgcctttc ccggcagcca gaccgtccgc tttaatgacg attggcgcgc
    12001 ctttctcacg cagatacacc agcgcaggtt ctacctcggt gaagttctgg tattccgccg
    12061 tagggatctt atggcgggcc aggaaatctt tggtaaacgc ttttgagcct tccagttggg
    12121 ccgcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta tcgaccacgc
    12181 ctttcaccag cggcgcttcc gggccgacga tggtcagatc aatcttttcg ttttgtgcga
    12241 aatccaacag cgccgggata tcggtcacgc cgatagcaac gttttgcagc gcgggttcca
    12301 gcgcagtgcc tgcattaccc ggagcaacaa aaacagtctc aaccagcggc gactgggccg
    12361 ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact ttcatctatt
    12421 gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac cgtgctcgtc
    12481 ggcggcggca atcacttcgt catcacggat ggaaccgcca ggctggatta cgcaggtcac
    12541 gcccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt cagaagccat
    12601 cgaggaacct ttcacttcca ggccttcatc ggcagcttta ataccggcga ttttcgcgga
    12661 gtacacgcgg ctcatctggc ccgcgccaat accgatggtc atattgtttt tggcatagac
    12721 gatagcattg gatttcacga atttcgccac tttccagcag aacagcgcat cacgcagttc
    12781 ctgttcggtc ggctgacgtt gggtgacgac gcgcagttct tccgcaccga ccatccccag
    12841 gtcacgatcc tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac ccggaacacg
    12901 ctcgccccac tgaccgcagg tcagaacgcg tacgttctgc ttggcggcgg tgattttcag
    12961 ggcttcttcg ctggcggaag gcgcaataat cacttcgaca aactgacgag aaatgatggc
    13021 ctgtgcggtt tccgcatcca gctcgcggtt aaaggcaatg atgccgccga atgcggaggt
    13081 tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga tagctacacc
    13141 gcaagggttg gcgtgcttca caatcacaca tgccggctcg gcgaactctt tcacgcactc
    13201 cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gctttaccct gaacctgggt
    13261 tgcggtagca acggaggctt ctttcacatt ctcttctata tagaaggcag cctgctggtg
    13321 gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca gcgtgcgtgg
    13381 gaagcgaccg gcggcttctt tgctttcacc gtggtacgcc ggaaccatgc tgccgaagta
    13441 gttggcaatc atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg cgaggtcgaa
    13501 acgggttgca agcgtcagcg atccttcgtt gtcatccatc tctttaataa tggcgtcgta
    13561 gtcgctgctc ttcaccacga ttgcgacatc tttatggttc ttagcggcgg agcgcaccat
    13621 ggtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcagc cttcacgagc
    13681 aacagtctgg gcgaacggat acaggttaac aaccaccata tcgataggct ggatctgatg
    13741 ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat gtactttcgg
    13801 atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat cggaaacttc
    13861 ggttaccggc agaccttttt ctgctaacag acgggcagtg ccccctgtag acagcagctc
    13921 cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt cagaaacact
    13981 gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg gtttgactat
    14041 tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct aacggggcat
    14101 ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga atttttctct
    14161 ttcaatggtg atcacaattt tgacagtggt taccgtggga aaaatacaga aattacattg
    14221 atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct ggggaatgca
    14281 gcagtcagtc atttttctgc aatttttcta ttgcggcctg cggagaactc cctataatgc
    14341 gccttcatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag
    14401 a
//
LOCUS       DADLCQ010000104        14250 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000104 DADLCQ010000000
VERSION     DADLCQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.104"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..104)
                     /locus_tag="JQG95_002992"
     CDS             complement(<1..104)
                     /locus_tag="JQG95_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY5889481.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JQG95_002993"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JQG95_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY5889482.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1353..2003
                     /gene="csgD"
                     /locus_tag="JQG95_002994"
     CDS             1353..2003
                     /gene="csgD"
                     /locus_tag="JQG95_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY5889483.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2008..2397
                     /gene="csgE"
                     /locus_tag="JQG95_002995"
     CDS             2008..2397
                     /gene="csgE"
                     /locus_tag="JQG95_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY5889484.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2422..2838
                     /gene="csgF"
                     /locus_tag="JQG95_002996"
     CDS             2422..2838
                     /gene="csgF"
                     /locus_tag="JQG95_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY5889485.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2865..3571
                     /locus_tag="JQG95_002997"
                     /pseudo
     CDS             2865..3571
                     /locus_tag="JQG95_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3704..4195)
                     /locus_tag="JQG95_002998"
     CDS             complement(3704..4195)
                     /locus_tag="JQG95_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY5889486.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4297..4851)
                     /locus_tag="JQG95_002999"
     CDS             complement(4297..4851)
                     /locus_tag="JQG95_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5889487.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4875..5612)
                     /locus_tag="JQG95_003000"
     CDS             complement(4875..5612)
                     /locus_tag="JQG95_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY5889488.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="JQG95_003001"
                     /pseudo
     CDS             complement(5667..6605)
                     /gene="ghrA"
                     /locus_tag="JQG95_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6840..6927
                     /locus_tag="JQG95_003002"
     tRNA            6840..6927
                     /locus_tag="JQG95_003002"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6874..6876,aa:Ser,seq:gga)
     gene            complement(7077..7358)
                     /locus_tag="JQG95_003003"
                     /pseudo
     CDS             complement(7077..7358)
                     /locus_tag="JQG95_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7640..8644)
                     /locus_tag="JQG95_003004"
     CDS             complement(7640..8644)
                     /locus_tag="JQG95_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY5889489.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8716..9276)
                     /locus_tag="JQG95_003005"
                     /pseudo
     CDS             complement(8716..9276)
                     /locus_tag="JQG95_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9361..9558)
                     /locus_tag="JQG95_003006"
     CDS             complement(9361..9558)
                     /locus_tag="JQG95_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY5889490.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9570..10061)
                     /locus_tag="JQG95_003007"
     CDS             complement(9570..10061)
                     /locus_tag="JQG95_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889491.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQISAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10058..10432)
                     /locus_tag="JQG95_003008"
     CDS             complement(10058..10432)
                     /locus_tag="JQG95_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY5889492.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10479..10853)
                     /locus_tag="JQG95_003009"
     CDS             complement(10479..10853)
                     /locus_tag="JQG95_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY5889493.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11016..11237)
                     /locus_tag="JQG95_003010"
     CDS             complement(11016..11237)
                     /locus_tag="JQG95_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY5889494.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11300..11776)
                     /locus_tag="JQG95_003011"
     CDS             complement(11300..11776)
                     /locus_tag="JQG95_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY5889495.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11792..12265)
                     /locus_tag="JQG95_003012"
     CDS             complement(11792..12265)
                     /locus_tag="JQG95_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY5889496.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12607..13428)
                     /locus_tag="JQG95_003013"
     CDS             complement(12607..13428)
                     /locus_tag="JQG95_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY5889497.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13528..13701)
                     /locus_tag="JQG95_003014"
     CDS             complement(13528..13701)
                     /locus_tag="JQG95_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY5889498.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13821..>14250)
                     /locus_tag="JQG95_003015"
     CDS             complement(13821..>14250)
                     /locus_tag="JQG95_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889499.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3642 a         3489 c         3411 g         3708 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tatttttata
     1021 tgtattatta gtaagttagc accatttgta tgatttttta aaattgtgca ataaaaacca
     1081 aatgtacaac ttttctatca tttctaaact taataaaacc ttaatgttaa cattttaata
     1141 taatgagtta cattttgtta catgtttaac acttgattta agatttgtaa tggctagatt
     1201 gaaatcagat gtaatccatt agttttatat tttacccatt tagggctgat ttattactac
     1261 acacagcagt gcaacatctg tcagtacttc tggtgcttct attttagagg cagctgtcag
     1321 gtgtgcgatc aataaaaaag tggggtttca tcatgtttaa tgaagtccat agtattcatg
     1381 gtcatacatt attgttgatc actaaacctt ctttgcaggc gacagctctc ttgcagcacc
     1441 ttaaacaatc gctagcaatt acaggaaaat tacataatat tcaacgttct ctggacgata
     1501 tctcttcagg ctctattatt cttctggata tgatggaagc ggataaaaag cttatccatt
     1561 attggcagga tactttgagc aggaaaaaca acaatatcaa aatattgttg ctaaatacgc
     1621 ctgaagatta cccgtaccgc gacattgaaa actggcctca tatcaacggc gttttttatg
     1681 ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg cgtcctgcgt ggcgaatgct
     1741 actttacgca aaagcttgcc agttacctga ttacacattc tggtaactat cgttataaca
     1801 gcacggaatc agccctcctt actcatcggg aaaaagagat cctgaataag ctgcgtatcg
     1861 gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag cgaaaatacg gttaaaacgc
     1921 atctttataa tcttttcaag aagatagccg taaaaaaccg gacacaagcg gtttcctggg
     1981 caaacgataa cctcaggcga taaagccatg aaacgttatt tacgctggat tgtggcggca
     2041 gaatttctgt tcgccgcagg gaatcttcac gccgttgagg tagaagtccc gggattgtta
     2101 actgaccata ctgtttcatc tattggccat gatttttacc gagcctttag tgataaatgg
     2161 gaaagtgact atacgggtaa cttaacgatt aatgaaaggc ccagtgcacg atggggaagc
     2221 tggatcacaa taacggtcaa tcaggatgtt attttccaga cttttttatt cccgttgaaa
     2281 agagacttcg agaaaactgt cgtctttgca ctgattcaaa ctgaagaagc actaaatcgt
     2341 cgccagataa atcaggcgtt attaagtacg gacgatttgg cgcatgatga attctaaata
     2401 aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag tagttctact catgcttatt
     2461 tcgccattaa gttgggctgg aaccatgact ttccagttcc gtaatccaaa ctttggtggt
     2521 aacccaaata atggcgcttt tttattaaat agcgctcagg cccaaaactc ttataaagat
     2581 ccgagctata acgatgactt tggtattgaa acaccctcag cgttagataa ctttactcag
     2641 gccatccagt cacaaatttt aggtgggcta ctgtcgaata ttaataccgg taaaccgggc
     2701 cgcatggtga ccaacgatta tattgttgat attgctaacc gcgatggtca attgcagttg
     2761 aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc aggtttcggg tttacaaaat
     2821 aactcaaccg atttttaagc cccagcttca taaggaaaat aatcatgcag cgcttatttc
     2881 ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc cccgcctaaa gaagccgcca
     2941 gaccgacatt aatgcctcgt gctcagagct acaaagattt gacccatctg ccagcgccga
     3001 cgggtaagat ctttgtttcg gtatacaaca ttcaggacga aaccgggcaa tttaaaccct
     3061 acccggcaag taacttctcc actgctgttc cgcaaagcgc cagggcttac aaaacctgct
     3121 taacgagcgc aaggttattc gtgcggcaca agaaaacggc acggttgcca ttaataaccg
     3181 aatcccgctg caatctttaa cggcggcaaa tattatggtt gaaggttcga ttatcggtta
     3241 tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat tttgggatcg gtgcagacac
     3301 gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc gtcaatgtga gtaccggtga
     3361 gatcctctcc tcggtgaaca ccagtaagac gatactttcc tatgaagttc aggccggggt
     3421 tttccgcttt attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcaaacga
     3481 acccgttatg ctgtgtctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga
     3541 tggtatcgac cgtggtctgt gggatttgta aaataaagca gaacggcaga atgacattct
     3601 ggtgaaatac cgccatatgt cggttccacc ggaatcctga cagataaaaa tgccggaatt
     3661 aataatccgg catttactgt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga
     3721 tttcctgctc acggtgagcc gtctttgcac tacgcgccgc cagccacagg ccgctgtttt
     3781 tcattgcgta accaaatacc agcccaagcg ccagcgaagg taacaccagt ttccagtcac
     3841 cctgcccggc aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca
     3901 gctgtttggc ctgaatacac atcagaaacg cgacaatacc ggttatgaca taaccgagaa
     3961 tttccagatg tggtgccagt gcactaccgt aaataatgac catcgcccac accacgccac
     4021 taagcagggt tgcagcggag atcgccagcc ctttcagccc accttgcggg caagcaaagt
     4081 aggccgtaca gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc
     4141 cccagatacc ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc
     4201 gcatgatagc agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa
     4261 attatctgtt gcagttatga gtgacgatga tcacatttac tcttcagaat cttcttccag
     4321 ctcgtcccac attgcactaa tggcatcacg ggttagcggt gccatggtgc gccagaaagg
     4381 cgtggttgca tgggcctcca ctttgccgag gaacgcacca caccagggta acagatactc
     4441 actgaacagt gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga
     4501 agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta tccgccagtg gcatccctcg
     4561 ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc tcaacccatg cgctacgata
     4621 tggcggcacg gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt
     4681 catatcacag cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc
     4741 agccagtttc ccctcacgaa tcagagtaaa cagcggcacc agtaaaggat cttgcggttg
     4801 gcggtaatag agcgaaccca gcacacgaca gaggatagaa aactcgttca ttaatatatt
     4861 tccattacta gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca
     4921 aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc gctctagcgg aaaatctacc
     4981 gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg taaacgcggt gtgagaatcc
     5041 gagcctaatg ccacccaacc tccagcatca cgtaccgctg cagctaccgc acgacagttg
     5101 tcttcactgc ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga
     5161 tgtttcgcgg ctgcttcagc aacggcttta acatctattt catatttcgg atttccggga
     5221 tggcttatta tatgcacatt gccgctggcg atagtggaga tcatcgcttg tgtattggtc
     5281 gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgag
     5341 tcaaacattt taccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcaatg
     5401 ccgcgcagga tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc
     5461 cagtgatgcg gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg
     5521 cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg tgtgctggca
     5581 acggtatgca tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca
     5641 aggatagcag gaatcctgat gctttattag tagccacgtg cgcggtcgac ttgcccgcag
     5701 accctctccc ctttttcgag ctgggcaata gtgcgagaaa tgtactccac agcttcagcg
     5761 ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga
     5821 ctttcaggcg gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttcccg
     5881 ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa caccacgcgc caggttgagg
     5941 agatacgcgc catcaggtaa tttttcgagt aattgttgat taataatgcc gacggtttca
     6001 ggggtattcg gtaacaaatt aatcaatacc cgacattggc tcagaaatgc agacagttct
     6061 tcccgtccgg caaagctttg cacgccaggc cacgatttac gggttcgact ccagcaacgc
     6121 agcggaaagc gctaggtttg cagactctga gcaactttac agcccagtac gcctgcgccc
     6181 aaaatgccga tggtaaaatc ttcccgatga tattcaggca gcggttgcca atgcgaacta
     6241 ttttgctgga tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca
     6301 tattcctgca tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggg
     6361 ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt
     6421 gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa caggaggatg ccagactaaa
     6481 acataatcag cagagtcatt atctccgctt ttccatgccc tgactcttgc ctgaggaata
     6541 gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata
     6601 tccatactga ctcccgaaaa gcgttgtgcg aaatttattc gcacttatcg ttatgatcta
     6661 caaaggccac cagcataaca aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt
     6721 aaaaccacac attgattgaa tgtttgaata aacgcgcgat tttttcaaaa agtttgttga
     6781 cctcaggtca tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg
     6841 gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg
     6901 ggggttcgaa tccccccctc accgccagat tataagtgca tgttattgtt taacatgcgc
     6961 ttttttattt ttccttccag acagtatgcc atcgggtatg ccatctgaaa ccggtttata
     7021 tgccccgcga tcatatctta tcggacaatt tccgatcttt gagcatactg gcaaagctat
     7081 actgccagca tatcaggata gtgttttgcg ataatatcat tcagtctgtc aaccagttct
     7141 ggttttctga acgtgatatg cgcggagccc ttcttaaagt atctgatgct gaacatctca
     7201 tcttcatagc tctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg
     7261 tttccccggt tgtcggggat tggtttaccg ctgaacaggt gcaacattct ttccagatcg
     7321 gcgagtcggt cagcctgttg tccggtgata agatgaaatc tatggtcacc cccgtttttg
     7381 caacactgat ttttgattaa tggttggctt gcttaaatct atccggcgtc tctgtgggca
     7441 aggatgcccg cgccccgatg agttccgcac ctgataatcc taaaaatccc ctcggcttca
     7501 gtgagccatt tcatctgtta ggtttttaat caggtcgatt tgccgttcat gtcatcaata
     7561 atcagtcttc gcaaaaccag aaaggtaact tgtatcttat tgtttaaaat aaactaattg
     7621 agttatatca gcatctatat caggcctgat attctttttt ctgagtagtg attgcccagg
     7681 caattcgagc cagtttattt gccagagcgc aggtaaccac attcgaatgc ttcttgttga
     7741 gttgctcccg aacccactca gcaagttttc catgcttgcg ttcaagtcgc attatgaatg
     7801 agcgggcaca ctgaaccagt aaccggcgta aatttttatc acctcgctta ctgataccca
     7861 acagagtcga ttttcctcca gtgctatact gccggggaac aagtcctgtg gaagctgcaa
     7921 aatccctgct gcatgagaac tgtttcccgt caccaagttg cgacgatagt aagctggcag
     7981 taatcggacc aacaccagga attgtcataa tgcgctgagc ggtatcatca gcgttgattg
     8041 actgacttaa ttctgattcc agctctgcta tttgctcaac aaggtaaaag tagtgggtat
     8101 gcaacctcac caaaaggcgg ctcagatatt cggggatttc gtgttcagcc aacacgagag
     8161 acaaccgttt tattacagca tcacctatcg gcaggctgat tccaaattcc agcaagaaac
     8221 cgtggatctg gttggttgtt ttcaccttat ctctgatgag cgattctctg acccggtgta
     8281 acgccctcat ggcctgctgg gtctcagttc ttggctggac aaaacgcatt gatggtcgtg
     8341 aagccgcttc gcatatggct tctgcatcaa caaaatcatt cttattactt ttgacaaacg
     8401 ggcgaacgaa ctgtggagaa atcagcttag tttcatgccc aaaatcagag atacagcgag
     8461 ccataaagtg tgcacccgca caggcttcca ttacaacaac tgagggcgga catgtcgcca
     8521 gaaactgcat cagtttagta cgggtaaatt ttttacgcag aagagcctta ccagacttgt
     8581 cttgacaatg aatgtggaag gagtgctttc cgagatcgat accaataagt gtgatgttct
     8641 gcatgatggt ctctccgtta aagggattta ccttaacagc gtactgctgt taaggtgggg
     8701 gtgaccatat cattatcccc accggtccca tctcaccaga ttgttgacga taattttact
     8761 gccaaattta caggggcaat tggttttgta attccagttc agccctctga agacgttgat
     8821 cacccctcgc tcaaacacct catccttatt ctggtgcagt tgttcaaatg tgctcagaat
     8881 gttcgcttcg ctgatttccg gaaaattatc gtactccagt gaccggtacc atgtatcacg
     8941 ggcctgcgta tccattaatg acagcatccc ggaacgttgc atcaggtcac gccagatttc
     9001 gcgatcaaga ttccgggtaa tggctttcat tgctgtttcg ggtttctcca tcagccagca
     9061 gccgcagcga aaatcctgtt tcatggccca gtccggggca gttttacctc cgatgctttc
     9121 cgtcagcacc gagatttcag ccagttgtcg gataagtgtg tcgatttgca acagtgtact
     9181 ctttcgtaca gccagaatgt ggcgaatgct ggttgagcag ataacgtccg tatgcccgtt
     9241 gaccacttcc ggacgggaga ttgtgatatc tgacataatc gatattcctt tttgcagata
     9301 aatgaaaacg ccagccctgt gcaggctggc gtcagaggaa tgaagtctgg tgagtgagct
     9361 tcaccggttc tggtgcagaa gctgcagatg acgcagggta cggacatcct cccgggcctg
     9421 ttcgatacag ggcaacagtg ctgctgaatc cgttttatcc tcatcgttgt caaagataat
     9481 tcccgatccg cagtcgatat tgtcctgcag ccacgcaatc agaatatcca gcgctgtttc
     9541 cgtggttaat gatttcatgt tgttcctggt taccagtcga aagtgggtaa acccggcagg
     9601 cattcagcgc tggcatccag atgaatgaga ctgacgccat aacgccggat gagtgtgacg
     9661 accagacgac ggaacgtttt tgacagcccc aggcgtttaa gccgcagaac cgggtatgac
     9721 cacgcatcgg tacgtaacag atatccgcta cctgtaaaat gaatccattc tgattcaccg
     9781 aagtcactgg tctggtgtga cagcgagtac aaccaggcgt tgtccgtttc cgtgatatgt
     9841 gcggtactgc agcgtatgcc ggtaagggta gcaaatggtg gcgtgcattc tgctgagatc
     9901 tggtcagatt catcaacaac acgaagggag tagccgttat cgcagaccat gttaatcagt
     9961 tcagcgagct caataccatc gacgtcaacg acaatgcgcc ctatgttcag tgcctgtacg
    10021 ttaacgctgt cggcttccag tgtcagggcc agtttcatca tttcgcctcc ggatacttac
    10081 ccagggtaat gttatttacc gttctgtaat tgtcgcgggt catcaggccg gtcgcccggc
    10141 gagcccggag gatatcaata ctgtttatta actgagagcg gggacaggtg ctgaatcccg
    10201 gctggtcggt acgcaccagc gcgtattttt ccacgagaaa attaactgca tcacacagtg
    10261 aaatgcctgc ctcaatatgc tgctcaatca cacgttcatc gacaaacggt gtgtcattca
    10321 gtgtgaggcc ataatgctgg tccagcagtc ggatgagcag tgtctgccag atggtgacgg
    10381 gagacgggca gcgagatacc tcccgtacat gtgtgtcgga taatgttttc atcttaagtt
    10441 ctctgtaact ggtagtgcta aaggtgagag cttcggggtt atgaggtggt tgcgggtgct
    10501 ggtgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag
    10561 gtcagccctt ttgcatacag cgtgacggta tgctgatatc ggggattcag ttcaccgctg
    10621 gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtccag atggtatgca
    10681 tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg caaggtaata caggcggtta
    10741 ccctcttgca ccagacgagc gccaaaacag ggcctcactg tgcagggaag cccccaccag
    10801 gggcggtcgt gattgtcatc gggatgagtt atcccggaga gtttgtctga cacgataaaa
    10861 tcctctctga aaagtggtta agaatggttt aacgatgaca ggtcagccgg cgcgctgacc
    10921 ggtttcggtg aagatgtatg cattgatgag aatggcctca tccacagcgg cgtctgaggt
    10981 catctggtcg tactcgtttc ccgggtgacg gaacatcagt tcagggtgac actcatcagc
    11041 cggacatacg ggccaaaact gtccttacgg cgttctgcaa acacggcaag aacgccggga
    11101 atatcctgca cttcacgacc agtatacgct tcagcactgc cgtgccagcg atacttaccg
    11161 gtacagaacg gaaaaagacg ggatgcagga tgctgttggt gaatacgcat ggcttcacca
    11221 cgggtgatga ttctcatgat gtgatacctc tgaagacaga agataaaagt gaaaacaggt
    11281 gtgatgtggt tgtgacgggt taaagcagac catgttcggc aaaggagaaa acctggttgc
    11341 caccgactat cagatggtcc ggcacccgga tatccaccag gcccagtgcc tgtaccagac
    11401 gttcggtgat aagccggtct gccttactgg gtgtgacttc accggacggg tgattgtgcg
    11461 ccagcaccac ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc cggggatgaa
    11521 cttccgtacg gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc tgattctggt
    11581 tgttcagata cagcacccgg aactcttccc gctccagtcc cgccatgttc agaatcagcc
    11641 attcccgtgc cgcacgggtg gaggtgaagg ccacgccggg ttcatgaaga tggcggtcca
    11701 gggtttgcag ggcccgcaga atgagactgc gctcgccggg cgtcatgttt ccgggcagaa
    11761 aggaaagctg ctgcattgtc cctgcctcca ttcagtcgat gatgcgcata atggcgctgc
    11821 attccggatg ctgcagggcg taatcccgca gccggtaata atggaccgtc atggcataac
    11881 actccgtacg acaggcatga tgactgtacg tcatcagaca ggcagcaatg ccggcggctt
    11941 ccgggctcat ttcagcgcgg ttaccgttca tggcattgaa cagtacccat gtttcgtcat
    12001 catcgtcatc cggttcgggt gccataaatg ccccgccgtt gttcagggtg tacagattcc
    12061 agataccacc gcagtagtct tcacacagac ggtccatcca gccgaagaca cggggttcca
    12121 gggtgaccca ctgtggaatg aggccaaaat gctgcggcca gaagctgatg cgctgttcat
    12181 cagggacggg agtggcaacc agttgttgca ggccagtctc cggtgcacag gccgaagcgg
    12241 tggtgttctg agacaatgtt ttcatgtatg aaatccttgt aatgtgaata aatgagaggg
    12301 agtaaaggtc gggagctggc aacatcacgt tcatcactga ccgtgatgac ccggtaatat
    12361 cagggatgaa tgatattcgg ctgaaattcc ggacagccgt acgcgtaacc gttccggtca
    12421 cagacggatg gccagatgcc attgcctgta tactcagtct gcacagactg atttctccgg
    12481 gacggtaaag cagacgggta caccaggcgc acaggtcatc atcagaaatg gaacgacacc
    12541 ccggcagcgc ctccggggcg tcgggtgatt ctggtgcctg aatcacgcct tcagggacat
    12601 gctgcctcag cgcaggctct ccagcagcgt ttctgccatt acccacaatg cgcggttgag
    12661 cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg atacgttttc cttttgcact
    12721 gcggccggaa atcccgcctt tcagcatatt ctcctggatg gtctgatatg cgctccacag
    12781 gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg tcagcggtgg tgactggctg
    12841 atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg
    12901 cggcaggacc agcgactgca tggcatcacg cttctcctct atccggtcaa acacgcccac
    12961 cacctcgtaa gccccttcaa taactttctc caccacattt ccccggtgtg gaacacgcaa
    13021 ttctcccaga gactgaccgc agaccagtcc attggtacaa atggcacgaa aatatccggg
    13081 taacatctgg tagctggatg aaccgtcatg agagttgagc agaataattt cagggacatg
    13141 ctgaccggtt atctgtccgg cccgccgcag acgcagcatg tgttttgtgt attcccggcg
    13201 acccgggtca cgcacacgtg tctggcaggc gaagaatggc tgaaagcctt cccgctgcag
    13261 gctttccagt acggtgatgg tgggagtgta cgcatagcgt ttactgcggg aagtatgccg
    13321 gtcttcaccg aaaatactgg gaacatagtg catcagttct tcgtgtgtca gcggacggtc
    13381 acggcgtatc tggtttgcag cataaccaaa acgactggct agtcgcataa tttgctcctt
    13441 atcggtggtt aagatttact ggtgtaataa atgaaaaagc cacgtctccc ggagaagacg
    13501 cggcctgatg gacgaaatta atgaggttta ttgtctgaga agaccgtcac tggcgaacct
    13561 tccggcgtca gtctctgcgt tgcggataac ccgacggaaa tgagttccct ggtcatcgtc
    13621 gataaacaca ttgcggtaag ctgttgtgat gacaacagcc tgctcgcgtg agaaagggac
    13681 ttcgggcaat aaacgtgaca tacaaccaga cctgtatttg gttccctgaa aatataagtc
    13741 cctgtcatca tggcagagcc gtatttactt ttgatatcaa taaattaata acgcgaagat
    13801 gaaccgcata acttacgcct ttaatttgtt ttgtttacgt agtccctgac caggtacgcg
    13861 aattcgtgaa cgccagtttc accaaaccaa atcaggtaac cagggtcgat gttgtaatag
    13921 taaccaacga ctttatgcgt ttttgggtta tagagtgtta cgatatagtc ccagccgcag
    13981 gcgtgggctt tacaaccgcc accttctacc caggtcgttc cgttaatgtt tacaggatac
    14041 gcaccaccat caggaccatc catatagccg tcttccccaa acattccgtt taacccaaca
    14101 gggtttaatg ctttgtcgat agcagctctg taggtagcgt caccttcgta taactcgtac
    14161 gttgttggac atgcaccagg ctggtcagcc cgggcttttg agtcgcattt aagtgtctga
    14221 tttacagtgg cggcgtttgt gtcaggactt
//
LOCUS       DADLCQ010000105        14149 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000105 DADLCQ010000000
VERSION     DADLCQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.105"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..455
                     /locus_tag="JQG95_003016"
                     /pseudo
     CDS             1..455
                     /locus_tag="JQG95_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            515..622
                     /locus_tag="JQG95_003017"
     CDS             515..622
                     /locus_tag="JQG95_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY5889500.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(603..1334)
                     /gene="yehW"
                     /locus_tag="JQG95_003018"
     CDS             complement(603..1334)
                     /gene="yehW"
                     /locus_tag="JQG95_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY5889501.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="JQG95_003019"
     CDS             complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="JQG95_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY5889502.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="JQG95_003020"
     CDS             complement(2258..3415)
                     /gene="yehY"
                     /locus_tag="JQG95_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY5889503.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALLVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JQG95_003021"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JQG95_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY5889504.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     FQVLESHGVPTVNKVQLGTTPVGRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JQG95_003022"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JQG95_003022"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY5889505.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JQG95_003023"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JQG95_003023"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY5889506.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JQG95_003024"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JQG95_003024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY5889507.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JQG95_003025"
     CDS             complement(9943..10530)
                     /locus_tag="JQG95_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY5889508.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JQG95_003026"
     CDS             10700..11278
                     /locus_tag="JQG95_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5889509.1"
                     /translation="MALNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JQG95_003027"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JQG95_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JQG95_003028"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JQG95_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY5889510.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="JQG95_003029"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="JQG95_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY5889511.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3361 a         3963 c         3564 g         3261 t
ORIGIN      
        1 cggcaatcta catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
       61 gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac
      121 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
      181 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
      241 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
      301 cctcgctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc
      361 tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag
      421 aacaaggcca tgatattttc ccactcggta tttaatgatt cgttaacaaa tgcgctttac
      481 tgtacaatcc tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat
      541 cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac
      601 ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg
      661 atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta
      721 agcccgatga tgatgggcgt acccagcgta ctggccccta ccgttgaggc gatcgtcgcc
      781 gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg agccgccagc
      841 ggtagctcga ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct
      901 gtcacgctgg gatcaatcgc tcccagcccg gcaagtgtca cctgcaggat gggcagcaca
      961 ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac cggaacggca
     1021 atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt ttccaccagt
     1081 gggcgaaatt ccgcgcccca cgggcgagta acagcaattc cggcacctgt gccaatgatc
     1141 accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag agctgcaaaa
     1201 ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg
     1261 ctgtaaggca gccagaaaat cagcgccaca aaaagagcaa tgagccagaa cagcggatcg
     1321 cgcaacatct tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgca
     1381 aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga
     1441 gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg
     1501 acgcacgtaa tccgccacgc tacgtaacga aagcaggtgc acccccagtt cactacgtcc
     1561 aaaaaactgg cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg
     1621 ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc
     1681 aatatcatga gtgaccagca caatagtgcg ccccagcaaa cggtgaatgc gcgtcatctc
     1741 ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa
     1801 gacttgcgga tcggcagcca gtgcacgcgc cactcccaca cgttgctgct gaccaccgga
     1861 aagctgatgc ggataacgct cacgcaaatt tgactccagc cccagtagcg ccattaattc
     1921 gtcgatacga tcgtcaatcc gcgcccgcga ccatttttgt aattgcggca cggtagcgat
     1981 gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat agcccatccg
     2041 gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc ggatcactcc
     2101 gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga
     2161 tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc
     2221 tttttgtgcg ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca
     2281 gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca attaccggga
     2341 tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac
     2401 caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt tgcaccatca
     2461 ccacccgcag gctgcgcaga aataccagta acgccagcgg taactgaaca tgcaggaaac
     2521 gctgcgcccc gctcatcccc atcgctctgg cgctctccag cacatcgcgc gggatctggt
     2581 tcaaaccgac taccacgcct cgcaccagcg gcagcaaggc atagagcacc agcgcaatca
     2641 gtgcgggtgt cattccggtt cctgctatgc cgagcttccc cagccacgga aaggccgtaa
     2701 ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc acggtctgaa
     2761 tgacattgag cagagaaaaa attgccccct gccgcgcagt ggaaaagtag caccagatgc
     2821 ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt
     2881 gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc agccagccag aaacctctgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg accgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg tgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgcc
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgaaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgctcc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggaaat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 caccaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac cgtcattttc ttgagcaaat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaatgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccaag ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtataatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca aactggccta tggctctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctgg cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc acagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccgcgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccacaccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaaggac ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgagaat atttcagtct ctcatcctgt tgcgctcctg tcatgtgcat tgcttcatat
    14041 aatcactggc gcaaggagcg cgcagggggc gaccaatcgc cgccgccccc tgcccccccg
    14101 ggctctggcg aacaaaatcg ccgctgcgcg gtgccctcgg cttatccct
//
LOCUS       DADLCQ010000106        13963 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000106 DADLCQ010000000
VERSION     DADLCQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.106"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JQG95_003030"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JQG95_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5889512.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JQG95_003031"
     CDS             2219..2767
                     /locus_tag="JQG95_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY5889513.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JQG95_003032"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JQG95_003032"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY5889514.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JQG95_003033"
     CDS             complement(3664..4854)
                     /locus_tag="JQG95_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY5889515.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLSIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQG95_003034"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JQG95_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY5889516.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQG95_003035"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="JQG95_003035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY5889517.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQG95_003036"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="JQG95_003036"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY5889518.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="JQG95_003037"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="JQG95_003037"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY5889519.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQG95_003038"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="JQG95_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY5889520.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="JQG95_003039"
     CDS             complement(13351..>13963)
                     /gene="ssuC"
                     /locus_tag="JQG95_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY5889521.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3552 a         3562 c         3525 g         3324 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gctaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgctgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcccggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgtcg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaacagcgt
    13741 accgagcgcc accagaaaga ttttcgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagcct
    13861 ttctccccag cgcgacagtc cgctaatcag tcccagaatc aggcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgc
//
LOCUS       DADLCQ010000107        13956 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000107 DADLCQ010000000
VERSION     DADLCQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.107"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JQG95_003040"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JQG95_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY5889522.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JQG95_003041"
     CDS             complement(1604..1777)
                     /locus_tag="JQG95_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY5889523.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JQG95_003042"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JQG95_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY5889524.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JQG95_003043"
     CDS             3415..3744
                     /locus_tag="JQG95_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY5889525.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JQG95_003044"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JQG95_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY5889526.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JQG95_003045"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JQG95_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY5889527.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JQG95_003046"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JQG95_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY5889528.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JQG95_003047"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JQG95_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY5889529.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JQG95_003048"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JQG95_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY5889530.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JQG95_003049"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JQG95_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY5889531.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JQG95_003050"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JQG95_003050"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY5889532.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3661 a         3454 c         3244 g         3597 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcgc tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaaagac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacagctgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag cggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cttggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgcttg agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccaag tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggtta accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgagcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga caacgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttaccccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtataacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttttcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaagct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg cagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaaacagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttcgctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcaga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADLCQ010000108        13532 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000108 DADLCQ010000000
VERSION     DADLCQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.108"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..890)
                     /locus_tag="JQG95_003051"
     CDS             complement(<1..890)
                     /locus_tag="JQG95_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5889533.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="JQG95_003052"
     CDS             1131..1931
                     /locus_tag="JQG95_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY5889534.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="JQG95_003053"
     CDS             2009..2779
                     /locus_tag="JQG95_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5889535.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="JQG95_003054"
     CDS             complement(2827..4185)
                     /gene="mltD"
                     /locus_tag="JQG95_003054"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY5889536.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="JQG95_003055"
     CDS             complement(4257..5012)
                     /gene="gloB"
                     /locus_tag="JQG95_003055"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY5889537.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5046..5768
                     /locus_tag="JQG95_003056"
     CDS             5046..5768
                     /locus_tag="JQG95_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5889538.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="JQG95_003057"
     CDS             complement(5765..6232)
                     /gene="rnhA"
                     /locus_tag="JQG95_003057"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY5889539.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6297..7028
                     /gene="dnaQ"
                     /locus_tag="JQG95_003058"
     CDS             6297..7028
                     /gene="dnaQ"
                     /locus_tag="JQG95_003058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY5889540.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAVF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7161..7237
                     /locus_tag="JQG95_003059"
     tRNA            7161..7237
                     /locus_tag="JQG95_003059"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7195..7197,aa:Asp,seq:gtc)
     gene            complement(7368..8414)
                     /locus_tag="JQG95_003060"
     CDS             complement(7368..8414)
                     /locus_tag="JQG95_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY5889541.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="JQG95_003061"
                     /pseudo
     CDS             complement(8425..9410)
                     /gene="tssG"
                     /locus_tag="JQG95_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="JQG95_003062"
     CDS             complement(9407..11290)
                     /gene="tssF"
                     /locus_tag="JQG95_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY5889542.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="JQG95_003063"
     CDS             complement(11306..11800)
                     /gene="tssE"
                     /locus_tag="JQG95_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY5889543.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11797..12627)
                     /locus_tag="JQG95_003064"
                     /pseudo
     CDS             complement(11797..12627)
                     /locus_tag="JQG95_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="JQG95_003065"
                     /pseudo
     CDS             complement(12614..13528)
                     /gene="tagK"
                     /locus_tag="JQG95_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3103 a         3522 c         3609 g         3298 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcgc tactgcctgc accaatcgtc aaactttggc ctggcttcag
     3061 cttagatccg cgcagtttgt tccactgctg caaatctttg gtgcttacgc cgagacgtga
     3121 agcgatgctt gaaagcgtgt cgccagagcg tacggtgtaa acacggctgt taagcggcgt
     3181 attgttggca accagcgtcg actgtacagc agcaatttcg cctgaagcga gagattcacg
     3241 cagttgatct gcatgcttct ttggcaccat cacgtactgc ggcccacttg cgcccagcgt
     3301 ggagcctttc acgccagcgt tgaatgtctt cagcttgctg acggaaatcc ccgccatatc
     3361 tgcaaccttc gccatttcaa ccgggctact caggtgcaca cgcgccagag cacggctttc
     3421 atcggtcgtt ggcagacgta cgccataacg cttgctgttt ttgagaatat cactcaatgc
     3481 cagcatttta ggcacgtact gcttcgtttc ctgcggcaac ggtaacgacc agaagtccgt
     3541 ggatttccca cgcgctttgt tcgttttaat tgccttcatg acccgacctt cgccgctgtt
     3601 ataagccgct acggtcagaa gccagtcgtc gtcaaacatt ttgttcagac gctgcatcat
     3661 attcagcgcg gcagttgttg aagcaacaac atcgcgacgc gcgtcataat tgcgggtctg
     3721 tttcaaacca taattgcgcc ccgtgctcgg aatgatctgc cagatgcctg cggcattggc
     3781 gccagacgtt gcgtgaggat caaaagcgct ctccactatg ggtagtagta ccagttccat
     3841 aggcatgtta cgttttttaa cttgccctgc tatccagtac atatacggct ctgcccgtaa
     3901 agttacatcg tggagatagc tcttattgcg taaatatttc tgtttctgtt cgcgaatccg
     3961 gtcattttcc ggaattccca tctttagctc gtcgccaatg aaagcccaca agtcaccatc
     4021 tggcgcgata gacgtcccat cgtccatcca tcgtgcctga cttgtaaact ttgctgcttc
     4081 cccttgacca gctgcagaaa ggctctgtgc gtgctgttga acgttgccgg tactctggca
     4141 acccacgagc aggacagagg cgagtaatat cgcttttgcc ttcatgtgtg tgtcaatagt
     4201 tgcttaaaag acgaccgatc ataacggcga acggagccga tgacaagaaa gttttatcag
     4261 aacctatctt tctttgacct taaccatgca aaacgctctt caggttgttg caataatgtt
     4321 tcttcattaa ttccattaat taaatcaata tcttccgttc ttaaaaaaac attaatttgc
     4381 cgctcatttt tcagaattac gggtagtgtt atttgatttt ttgcccgtaa ctccttaact
     4441 ttacgataat aatcatttat ggacaaatcg tgcggaaaaa tactcaaagc aaacttcata
     4501 tttgataacg tatattcatg agcacaacat accaatgtat cgtcaggtaa cgcacttagc
     4561 tttttaagtg attgatacat ttgtaaagct gtcccttcaa acaaccgacc acacccacca
     4621 gaaaacagcg tgtcgccgca aaatagataa ggtttactga agtaacagat atgtcctaaa
     4681 gtgtgacccg gtgtggtaat tacactaaat tcatgcccta aaacgaaggc agtttcgcca
     4741 tctttgacta cctgtgttgt tcccttatct tgtgtctctt gtggaccata caccacaatt
     4801 tgcggaaact tttccaccag ttcttttacg ccgccaacgt gatcgtggtg atggtgggtt
     4861 agaaatatgg cctccggttg ccagttattg gcggtaatgg cgtttaatac tggctctgcg
     4921 tctccgggat cgacaatcag gcagcgacct gcctcatcat tcaaaaccca gatgtaattg
     4981 tcatcaaagg cgggaatact gttaagattc atagattacc tctcagtgta aaacggaagg
     5041 ttgtgatgaa accggcaaga gtccctcaaa ctgtcgtggc tcctgattgc tggggcgatt
     5101 tgccctgggg agagctttat cgcaaggcgc tggagcgcca gctcaacccg tggttcacta
     5161 aaatgtatgg ttttcatctg cttaagattg gcaatttaag cgcagaaatc aattgcgaag
     5221 cgtgcgcggt ttctcatcaa gtgaatgttt ctgcgcaagg aatgcccgtc caggtgcagg
     5281 cggatccact tcatcttcct tttgccgata aatccgttga tatttgtcta ctggcacata
     5341 ccttgccgtg gtgcaccgat ccgcatcgtt tattacgtga agccgatcgg gtactgattg
     5401 atgatggctg gctggtgatt agtggcttca accccatcag tttaatggga ttacgcaaac
     5461 ttgtgccggt attgcgcaaa acctcgccct ataatagccg gatgtttact ctgatgcggc
     5521 aactggactg gctctctttg ttgaattttg aagtgctgca cgccagccgt ttccacgttc
     5581 tcccgtggaa caaacacgga ggaaaactat tgaatgcgca tattcctgcg cttggttgct
     5641 tacaacttat tgttgcccgg aaacggacta ttcctttaac gctaaatccg atgaaacaga
     5701 gtaaaaacaa gccacgaatt cgccaggcgg ttggagccac ccggcaatgt cgtaaaccac
     5761 aggcttaaac ttcgacttgg tagcccgtat cttccagtgt gggattcatc gccgcggcac
     5821 gagccagttc atcacagcgt tcgttttccg ggtgtccggc atggccttta acccattccc
     5881 atttgatttg atgctgcccc aatgcagcat caagacgttg ccagagatcg acatttttta
     5941 ctggtttttt gtctgctgtt ttccagccac gttttttcca gttatggatc cactgggtga
     6001 taccctggcg gacatactgg ctgtcggtac tcaaaatgac ttcgcaatgt tcttttaacg
     6061 cctccagcgc gacaatagcg gccatcaact ccatacggtt gttggtggtg cgggtgtagc
     6121 ctgcgctaaa ggttttctcg cgtccgcgat agcgtaaaat agcgccgtaa cccccaggtc
     6181 caggattacc cagacacgaa ccatcggtga aaatttctac ctgtttaagc atctctggta
     6241 gacttcctgt aattgaatcg aactgtaaaa cgacaagtct gacataaatg accgctatga
     6301 gcactgcaat tacacgccag atcgttctcg ataccgaaac caccggtatg aaccagattg
     6361 gtgcacacta tgaaggccac aagatcattg agattggtgc tgttgaagta gtgaaccgtc
     6421 gcctgacggg caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag
     6481 cctttggcgt acatggtatt gctgatgaat ttttgctcga taagcccacg tttgccgaag
     6541 tagccgatga gtttatggac tatatccgcg gcgcggagtt ggtgatccat aacgcagtgt
     6601 tcgatatagg ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca
     6661 ataccttctg taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaaac
     6721 gcaacagcct cgatgcgcta tgtgcccgct acgaaataga taacagtaaa cgaacgctgc
     6781 acggggcatt actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc
     6841 aaacgtcgat ggcttttgcg atggaaggag agacacagca gcaacaaggt gaagcaacaa
     6901 ttcagcgcat tgtacgtcag gcaagtaagt tacgcgttgt ttttgcgaca gatgaagagc
     6961 tggcagcaca tgaagcccgt ctcgatctgg tgcagaagaa aggcggaagc tgcctctggc
     7021 gggcataaat acatgtaaaa ggcgctaaaa atagcgactt gggcgatttt tgcagcaaac
     7081 gattcaaaag atgagaaaaa tcgttgacga aggtcgaggc aatccgtaat attcgcctcg
     7141 ttcccaacgg aacacaacgc ggagcggtag ttcagtcggt tagaatacct gcctgtcacg
     7201 cagggggtcg cgggttcgag tcccgtccgt tccgccaata ttcagaaagc ccgaatcagt
     7261 aatgattcgg gctttcgtcg ttttggtgaa gtgagaattt ttcactccga agtaaaacgg
     7321 atatgattta aaaaatggcc agacattata aattctggcc acttctgtta ctccagcgca
     7381 tcccatttct ccagcagttc tactggaatt tcatttatta cctccgagac gcgttttccg
     7441 accaggcgtt ctgcctggcg cagcaaagga atggtcgggc tgctcggttc gctgtgctca
     7501 aaccagcgac ggatgacacg caggcgttcc agcgcgtcgt ttcggtcgcg gatctgccgg
     7561 ggttctgcgc cggtcatcgg tgatatttcc tccgcgatat gcaccggtag cgcaatctgc
     7621 tctgcctgca tctgttcagt atgaacgatg gtggcgtttt ccggctgctc agcctgtggt
     7681 tgtggcagga tctccggcgt ggtgctctgc actgctcccg gtaacagggc cagcagttgc
     7741 gtcagacggg agaagtccgg tgcctggtca ttcagcgttt cacgcgcatg gcgttgcagc
     7801 cgctcagtgg tctctgctgc ctggcgaaag gcatccagcg gcagtgtgcc gcgcgcttcc
     7861 aggtctgcca gttgctggcg taccgattct ggtgccagcg catccgccgg gcgtaatgca
     7921 gatagcgaac gctccacatc ccgcacctga aggcgcatgg cagcattatt tgacagtgta
     7981 atgccacgaa tatccgccat tacaccttcg tggtccagca acgccgccag cgcgttactg
     8041 cgtgccaccg cagcatcttc ggcggtgata tcttcagtcg cgagcagttg aggatggatg
     8101 gcgtccgggt acgtggagca caacgtttcc agcagcgtga gcgcctccga cagtcccctg
     8161 gcaccggcct gctggatacg gcagcgcagc aggacaacca gaatgcggat atctttgctg
     8221 cgcatcagca gacgatgggc gtcccgctca agctccgccc agttaatgtt ttccggcgta
     8281 ctgacaaaat cgccatattg cgcttcttca cgcggtgcgg cgcgggtgaa cagcagcagg
     8341 tattccggat cgtactccag atccgggccg caggggcatt ccgccgaaat tgggtcagac
     8401 aggaatattt ccatttaatt atcctcagtg cgccaggtac tgttctggtt cgaaaatcat
     8461 gcccgtcacc gggttgtcgt gcatggcttt acccgcccat gcagtccaac ccagttggta
     8521 cggaccaccg gtaacagccg gaggggcggc gtggggcttt agctgtagct caatttccca
     8581 gcagtattca taaccactaa aggcgcggac cagttctgtc aggacgggca ggttattgct
     8641 gccaggcaga aaacgtcggt aatcctccag cgtcagcggg ccgataacca gccggaactt
     8701 atactgcatg tccggcacgg cctggccgat gagtgcacca ttgccaataa cggaagaggg
     8761 ggcaggcatc cccagccggg tgatctcatc gtccgccacg gctatccagt gcaggacata
     8821 ctcctgtatc cggaaaggta caccgaaata gtgcgccagc gtggtggcca gaccatccgg
     8881 attgcgggac tcacgcacca gatgggccga tgccgccaga cgagcgtgat ctggcaacgg
     8941 gctgtcagcg ctctcccgca aatcctgccc gctgaggctg gcgacataaa aggcaaagcg
     9001 gtcatgttgc ggtttatcca gaccgccccc agcggactgg gcgctgcgcc atgcctggta
     9061 aaactgcgtc agccagcggt gatgaaaaag atcagaaaaa cgggtcagcg tcggctttca
     9121 gtccggttac gcgccagttc ggtgtagtgc agcggcagtg gaccgttcgg cccccacagc
     9181 ccgaggctgt acagactgat gtgcaggcgc ccttcctgcc agcccacact ggcgatttct
     9241 cgtggcgcaa aggtcagcgc gggcgtttgt ccgaggcgaa atttttccat ccgtggttgc
     9301 caggtgttgc ccgccggaac ggtacacaac tgcacatcca cgcggcgcat caggttcaga
     9361 aaaccgtaac gccagggggg ggcctgcgcc tgagtgaaat cgttgctcat acgcttcccc
     9421 gctgtcccgc cctgacaggc caggtcataa ttttgccgcg ctgcatgctg tgcagcgtca
     9481 tctgcgagaa ggtattaatg gaaacatggc gggcgatata gtgttccagc accaggccga
     9541 acagatacgg actgatgccg gaaaaacctt cttcatccac cgtcagttca caactgacgc
     9601 cacgcccata gaccagtagt ccggaaccgg gcagacggcg ggtgaccggg gtcgttttac
     9661 aaccaatcag gctgcgcacc tggcgcgatt gcggactgtc atgagcagga ataaacaggt
     9721 tcagcagatc tcgcaacgcc tggccgccgg tacggtgatc cagatcggcc agcggcagat
     9781 aattaaaaga taactggcgg atcaaccgcc atgccatctc acgttcagcc agcgggggct
     9841 gcggcgagcg tggcgggcgg ataagcccta cgcccgccac cgggatggct gcatccacgg
     9901 ttagatcgtc ccggccattg cgggcaataa gacagggcag atcgcggtta gtgaccatgg
     9961 ccgtgatggt gatgtggcgc agattttccg ggtacggcgc ttcatactgg tcaaccagcg
    10021 acaggaacac ttccgagccc gtataggggg tacgggtgcc gtaacggcgg gcgttttctg
    10081 ataagcggcg gggctcccgg cgcagggaaa aataacgtcc gtgattgcct tcatcattat
    10141 tgcgggtgtg gtagagcggg cgaaaggcca tcttccgtgt ggtgtcggtt tccagccctt
    10201 ccacttcctg gaccgagaac acctcataat ccagcggatg ggttctgtcc accaccagat
    10261 gctgttccgt cgtgctgtgc gtgacatcga tccgggcggt gacgcgggga aacaggttga
    10321 taaccggggt acagaacagg ctgaactgtg ccgcatttgt ctggtggatc agccagtccg
    10381 gcggcaggcg gttcagcagg ataacaatct ccgccacgcc accgtcaatt ttctgtaatc
    10441 cggcagacag gcctgtcggg gtgaaaaaat agaagcgttc cggacaggca aaatattcat
    10501 gcagcaggtt atgaccgtga aacacgttcc acgccagcgg cagcagcccc tgatccggct
    10561 ccaggccttc gtacataacg ggctgttgca gattcacatc cagcgcgccg tcaaaatgcc
    10621 ccggaatgcc agccagggtc gcgatggcgc tggtatgtag aagttctaac aggtgtgagg
    10681 ccgtacgctc ctcgccgcag agataaaacg gcagcctgtc cagtccggca agctgactga
    10741 acgttagttc accaaaggtg cgcagggtaa tacgcagcgc cccggcgacg tgaatgttgg
    10801 ccggcaggta gcggtgcagg gcaggcatat ccggtggcgc ggcggtcagt cgcgcctctt
    10861 cgatggcgag cggccacagc gttacatcct ggctgctgcg aaactggcag gcagtgcttt
    10921 cgccttccgg aatggcggag aaaaaggctg tgtcgcgcgg gacggtaaat cctttcgcca
    10981 gatcgccttc ttccgtatcg ggatgcaact gcgccacgct cattgagggc gtgggtgtga
    11041 cgtaattggg gctgaccact tccagcaggc gctgggtgaa acgggggaat tcagcgtcaa
    11101 tttttagctg cgtgcgggcg gtcaggaagc tgaaggcctc gaccattcgc tctacatacg
    11161 gatcggcaat atccgttccc tgcatgccca gccgggcggc gactttggga tgacgggcgg
    11221 caaattcggc tcccgtttcc cgcagatagc tcagttcgcg gttgtagtat tccagtagcc
    11281 gtggatccat cggtctctct ctgaattaaa aaaacgtaat gcgactctgc tccatatcca
    11341 gcgcgctacg tacccggaat gcagtcggat acggctgcgt gaggatctcg ccgcgaattt
    11401 caaactgtaa tgtgttatgg ccactctggc ggcgctcatc ctgaagtggg atgatttgca
    11461 gagtggacgc tttcagccgg ggttcaaacc gggtgatggt tcgcaaaatg gccctgcgga
    11521 tatcgtccca cttgtgctca tacagaaagc tgcccgccag tggcggcagg ccgtagttaa
    11581 gtacggacgc tgccgcgtac ggataacgtg cagcatcaat atcatcttca tggctgacgg
    11641 tgttgagcag aaatgacaaa tcccggcgga tgatctcctt aatctgaacc ggactgacgc
    11701 tgataccgtg gtcgcgtttc tggtttgggg cgtcgtcaca caggcggtca aacagcgtgg
    11761 gcagcaggtg attgcctgga cgctggcggg aagcgctcac gcgtcgcttc cttcagcggt
    11821 gttaaaggta caggtggaca aatccagcag gctgatatcg ccgtgaccgg tgagccacac
    11881 cctctgaccc agcgcctgta ccagtgtctc tcccggaccg tcctgccaga ccgtttcccg
    11941 gcacagacgc agggagtcgg cagccttttc agagccgctg tagcggggaa acagctaggc
    12001 actgtgtgta tcgccattgt tcagggtgac gttgacgggt ttccagatca aatcgatcag
    12061 atgcgcaggc cgcggcgatt tcagcgaaca gatttgtgaa aacggcagcc agatatatgc
    12121 tccgcccgcg atcagttcga gtaccggacc ggtacgggag tcgctgtcgc taatccagtc
    12181 aaatgcgccg ccattccact gcccggaagt gtctgttatg gcctcaagcg cgcggctgcg
    12241 gtgagcatca gcttcctgcg cttcaccacg ggcgttacag gcgagggcgg tcagcagatc
    12301 ctccatccag gcgggcggcg ggagaataac accgggccgc tgttcgccct aaaagcaggc
    12361 gtggcggtat atctcacagc ggatcagctc gccaaatacc tgcgcttcgc gagtataatt
    12421 tgcgtccatc cgggcgcata gctggatctg ttgcagtgcc cgcgaccagt cgcctgccac
    12481 gcacaataac tgaaacaggc tgtggcggcg ggaggcctgc gctgggtgca tcctgacctg
    12541 ttgctctgcc atctgaatgc cctccaccac ggaataatcc cgcagcagcg ccgacaaggt
    12601 aatcggtagc tcgttattgt ttgtcatgat atatctctcc atgttgtgcc gggtttatcc
    12661 ggtaatggct gtcaatgcca atgatgcgat gctctttccg tgtcagatct ggcagtacgg
    12721 tgttatgcgc tgtttccggg gcgtattccg gggaaagtaa atgcaggata tcagggggag
    12781 tcttttcgac aggccagata gtttcatctt cgccagtggc atccagtaca ttgagaatag
    12841 cgtcaatgct taacccacca gtgaccatat cctgcaatgt ctcggcttcg gtgaccaggt
    12901 cacgcaatga atccagatcc tgcgaggagg gctcttcgtt aaaaggaaaa ggatcagcat
    12961 ggcgattaag atcttgttcg ggagataaca acgcctcgcg gtattcctga taaagtcggg
    13021 tcagggcatt tcctttttcc gcaccttctc cggtggagga ggctccatga acaggaatga
    13081 tatcgaatgg attttgtgat gtcagatgct gctgcctgaa ccaatccaga tccagatatt
    13141 cggttattgc ttccgacgtg atggcagtct gcgatatcgt ttttactgtg ctcaggttcg
    13201 gagatgatgg attgccatgt acaaggcact atgaagataa cccccattct atcaggtcgc
    13261 cttcattcag acgcatacgg tgatgaggtt ccacaatctg gtcgtttacc gtacagcaat
    13321 attcggtaga ctgattgata atccaccatg cttcttcgtg gcgggtcaga gtgagggcaa
    13381 tctttacatc atcattcacc ttaaaaacag gaatccaggg agaggtaacg gtgaagataa
    13441 tttgtgttcc ttcagtcgcg taaattgtgt tatcggcgat cccgtgcgcc tgtatttttt
    13501 ggagtcccca cgtaatattt ggtttcattg tg
//
LOCUS       DADLCQ010000109        13142 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000109 DADLCQ010000000
VERSION     DADLCQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.109"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(87..794)
                     /locus_tag="JQG95_003066"
     CDS             complement(87..794)
                     /locus_tag="JQG95_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY5889544.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1192..3327
                     /locus_tag="JQG95_003067"
     CDS             1192..3327
                     /locus_tag="JQG95_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY5889545.1"
                     /translation="MKSMNIAASSEVVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /locus_tag="JQG95_003068"
     CDS             complement(3376..4632)
                     /locus_tag="JQG95_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY5889546.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="JQG95_003069"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="JQG95_003069"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY5889547.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /locus_tag="JQG95_003070"
     CDS             complement(5978..6253)
                     /locus_tag="JQG95_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY5889548.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="JQG95_003071"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="JQG95_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY5889549.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="JQG95_003072"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="JQG95_003072"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY5889550.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /locus_tag="JQG95_003073"
     CDS             8213..8539
                     /locus_tag="JQG95_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY5889551.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /locus_tag="JQG95_003074"
     CDS             8723..9442
                     /locus_tag="JQG95_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY5889552.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="JQG95_003075"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="JQG95_003075"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY5889553.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11742
                     /locus_tag="JQG95_003076"
                     /pseudo
     CDS             10781..11742
                     /locus_tag="JQG95_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(11843..13102)
                     /locus_tag="JQG95_003077"
     CDS             complement(11843..13102)
                     /locus_tag="JQG95_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY5889554.1"
                     /translation="MLVFIGVPISFSIGIATVASMLLMFPWDIAAITVSQRLANGLDN
                     FALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISGSA
                     VAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVSVA
                     SLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLDALPSLLLVFIV
                     MGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGF
                     SVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPI
                     FLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLLFY
                     AALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3450 a         3174 c         3404 g         3114 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtga tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgctggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga cgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttagc tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgcct tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccatctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaagtggta tcccgacttt cttctcatcg ccgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttctttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cggggacgac gccggtctat ctggaagctt cacgtaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga cctgccgcgc ccgtttcgcc tgacgattat tcagctggga
     2041 acctatgacg gcactgtcta taacgcccgt caggtgatcg ataccattgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatccc gatgatggcg
     2161 gatagctcgc cgctgctgtt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 ttggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataacgc ctttatgctc
     2341 catgcttcta ccagcccttt ctatccgctg tttgctgcac tggatgttaa cgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtaagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatca gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ccgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtggcgat gctggcgcaa tttgaacagc atattgagga tgactcgtcg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatt gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcttccggc gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaatacc gcggcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtattt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcagtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg caccatgtta atggtgacgc tgtagatgat
     5401 acgtagcccg ttgctgcggc ttttcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaattgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatggtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaagcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagc tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaactgctg taataaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagttgcg
     6481 tcaggttatc ggcagcaatc tgccgttgtg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aacaggagaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgaccagc tattgttggt ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggcttcg aacgcgtgca aatcatcgcg cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccga ccgcgggcgt cacctgctcg ctcaaactcc
     6841 atagtttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgcgcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc accgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag tgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gacttcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agtgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc cgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgttcaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaatcgttga
     8341 tgactttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagtgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctac agagatgcac gatcgagtag gcgggataag gcgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcat atgctggcct
     8701 gatttgttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgtagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgag aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgacag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag cttaaagagc agatgaatcg
     9121 cattatcgaa acgcgcagcg atggcctgac gttccactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaactag tgaatcaggc aatgggcgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcttgggc ggtctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccatg caaagctaag ggataatgcg tagcgtccac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacgccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaactgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgattac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagtcggcat cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagattt tcaatcagta ctaatcacct cgtcccggca
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgcga gggttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggcg ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 catcagctta aaaaatcttg atgctaaggg taatcaggcc acttggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt
    11701 ttgatgaaaa ttgacgatat caaccaagct aatgaacgct agaaaaaaga taccgaacaa
    11761 ctgcggcgca atggggtgat taagtaactc atgttaagtg cctttgccgg ataatataaa
    11821 aagttatccg gcaaattatt ttttacatta gccctagtag ctgtggaatg aacaaggata
    11881 tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa aatagcagca
    11941 atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca aatagcgcac
    12001 tacccaccgg cggtgttaac agcccaatac aaaggttggc caccatcata ataccgaaat
    12061 gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta aagattagta
    12121 cagccggtgt catgtccata aagatgccga caattaataa gacgatatta atcagcagca
    12181 atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata taaggaatat
    12241 cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt aatacaatcg
    12301 aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt acttcccggt
    12361 aaatcagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct tcagttgcgg
    12421 taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg
    12481 catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagc gggtagccac
    12541 ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag atccctggca
    12601 aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa
    12661 ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg
    12721 tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg ccaccaacgg
    12781 cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt
    12841 tcacatgacc aagcgaacca ggaacgcggc caaccattac ctgggcgagg ttaatcaaac
    12901 gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca
    12961 acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt gccgcgatat
    13021 cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg
    13081 gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg atataactat
    13141 cc
//
LOCUS       DADLCQ010000110        13035 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000110 DADLCQ010000000
VERSION     DADLCQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.110"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            126..995
                     /locus_tag="JQG95_003078"
     CDS             126..995
                     /locus_tag="JQG95_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889555.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="JQG95_003079"
     CDS             complement(1198..1551)
                     /locus_tag="JQG95_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY5889556.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQG95_003080"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="JQG95_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY5889557.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="JQG95_003081"
     CDS             complement(3379..3672)
                     /locus_tag="JQG95_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY5889558.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="JQG95_003082"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="JQG95_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY5889559.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQG95_003083"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="JQG95_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY5889560.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="JQG95_003084"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="JQG95_003084"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY5889561.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /locus_tag="JQG95_003085"
     CDS             7587..8888
                     /locus_tag="JQG95_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY5889562.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="JQG95_003086"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="JQG95_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY5889563.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /locus_tag="JQG95_003087"
     CDS             9318..11015
                     /locus_tag="JQG95_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY5889564.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="JQG95_003088"
     CDS             11052..11627
                     /locus_tag="JQG95_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5889565.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="JQG95_003089"
     tRNA            11734..11809
                     /locus_tag="JQG95_003089"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
     gene            12409..12699
                     /locus_tag="JQG95_003090"
     CDS             12409..12699
                     /locus_tag="JQG95_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889566.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
BASE COUNT         3184 a         3339 c         3175 g         3337 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcca tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc gcgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctgg cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggctgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 aaatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccac tatttagaag
    11821 aaccagccta cgggctggtt ttttgctttc tggggttctg gtagatatcc ggtcactttg
    11881 aatgctgatg tcactgcttt acctcttcca tccagcctga acatttcttc taaatagccc
    11941 ccttctggtc actgtggcct tttctgtcaa acttcaaatc cccaatagac actatttcca
    12001 catgagcacc tggctaacgg attcattact gcacggatac atccggtctt tgtccggtct
    12061 gaagtaaaac gcataccacc tgcgttggcc cctcctcagt ccccccctac atcaaaccga
    12121 acatttttca ctctgattga tttagcttta gtcaactttt gggtgttatg cctactatat
    12181 caatatgatc cataggggat tatggccaga ctaccggtaa taccgagaga caggatctca
    12241 atcggagata gaacgttctg caaaacgggg cttacagaaa atccctaaat gacctgcaac
    12301 ctccagccca ttgcatatta tgataacagt gaatattttg gttaagtcgc gccccgcaaa
    12361 aggtggaaag caatttaaca aattgaatta aaagggttta accgcaaaat ggacgcaaca
    12421 ggcaacacga cagtcacctg cctgagtcac aaaatgaagt acaaagaagt cgcctgcggt
    12481 aagcagcgga tgcactggct ggcgctgcag ttcgtggatg agaacaacaa cccagtttcc
    12541 ggtctcaacg tgcagctgga gtatcatcca cgggcaatgg cggcagagct ggctctgtgg
    12601 gccagaggca gacacactca gtttgacccc acaccgccgc ccccacctgt cggtgtcact
    12661 gacagtcagg gtctggtacg gtttgatgac ttgtactgaa ttaaagcacc taaaacaaac
    12721 atttatatcg caacagttaa atagaatgga taatcggtaa taaaatggtc cttgagtgcg
    12781 ttagatcaac atcaaaataa ttaaaatacc catgcatttt tttaagatat ggttttagat
    12841 cagaatgaga ttaaaaggga gagaaagtca tagccttatt tgatgtcaga aaccaatgcg
    12901 tggaagttag agcattacgg aaaaaagcct gacatactta acgggcaggc atagaggtga
    12961 tctcattgaa tttgttagcg caatcagtag atcacaaaac tctatgcctg tcttcttttc
    13021 tggggactcc tcagc
//
LOCUS       DADLCQ010000111        12993 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000111 DADLCQ010000000
VERSION     DADLCQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.111"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..707)
                     /locus_tag="JQG95_003091"
     CDS             complement(<1..707)
                     /locus_tag="JQG95_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5889567.1"
                     /translation="MAINNFKPFALDPNANVTSQADWEALPALLSGFTAGKASSAQVN
                     KAIRQASFIAAALAQYTANKSRLDVLDDGDLNGFISKMGTAFGKDFQALDATLSALAG
                     LATGANKLPYFTGNDTAAQTDLTSVGRDIIGKNTIADILTYLGLGEAAKRNVGTGANQ
                     IPDMSLFASSNTATAAAQKFPSGLILQCGQLNGSPNVSSTYGMRFPMTFSRVLAVTVT
                     LNVTGASGQPTVSATNV"
     gene            complement(707..1387)
                     /locus_tag="JQG95_003092"
     CDS             complement(707..1387)
                     /locus_tag="JQG95_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY5889568.1"
                     /translation="MSKYTELITNYHATKPLFFDHIDLSTRPLIDVSSTMSGLITAFD
                     IDTAVGVQLDILGLWIGRSRIVSQPISGVYFSWDTDGLGYDQGIWKGPYDPDSGYTTL
                     SDETYRIILKAKIAINNWDGRNDSLPPILDAATEGSGLRMQIVDNQDMTISVWVFPET
                     DISDVSLELIAAIKQGYLTVKAAGVWAGDVETPSVEAPSEGSKFFGFDMDNEYIGGFD
                     VGAWGTIL"
     gene            complement(1384..2583)
                     /locus_tag="JQG95_003093"
     CDS             complement(1384..2583)
                     /locus_tag="JQG95_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY5889569.1"
                     /translation="MALNLDTLGLSATVTAQGISAPDYQTILDTLTSYFRQIYGSDAY
                     LEPDSKDGQMVALVALAVHDANNTAIGIYNSFSPTTAQAAALSSNVKINGITRKVATN
                     STADLLLTGTAGTTITNGSARDKNGIIWNFPASVAIGVDGTVLVTATCANSGSVAALA
                     GTITTINTPTRGWVSVTNPVAATVGSPAETDAELRIRQGQSVALPSITPFEGVDGAIA
                     NVAGVTRHKLYENDTGATDSNGLPPHSISAIVDGGDVTEIAQTIRGSKGQGTATYGKT
                     SVTVPDTYGNPHVISFSRSTDVPIFVAITLKVFTGYTSQIGEQIKQAVADYINGLTIG
                     DDVLLSRIYSPANLGVVSGGNARYYDITDLLIGKSSGSVSASNIDIAYDASASCSTAN
                     ISITVTS"
     gene            complement(2584..2937)
                     /locus_tag="JQG95_003094"
     CDS             complement(2584..2937)
                     /locus_tag="JQG95_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889570.1"
                     /translation="MRYRREDTEGDYTFGQGDDTFLIDSPECVAQAVKTRFELWRGQW
                     FLDLTEGTPYVQSVLGKQRSDVYILAIRERIQDTPGVLSILSFDTNYDGTSRRVTFTS
                     SIDTIYGQTTVTSEA"
     gene            complement(2937..3677)
                     /locus_tag="JQG95_003095"
     CDS             complement(2937..3677)
                     /locus_tag="JQG95_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAY5889571.1"
                     /translation="MPIPTQSQIGGEQQTAQAIADSVSTQMRVAMPGIIQSFDPETVT
                     CTVEVALRGIVGDGSTELKPLVDVPVIFPRGGGCTLTFPVKEGDECLLIFADRCIDFW
                     WQSGGVQETVDPRQHDLSDAFAIVGPQSQARKISGISTSAAQLRTDDGAAFVEVAAGH
                     NITIKTPGQLTATAEGGTTITSPTITLNGNVTINGNLSQGMGESGGTATMLGPVMVTN
                     DVTASGISVATHKHGGVQTGGGTTGGPQ"
     gene            3900..4190
                     /locus_tag="JQG95_003096"
     CDS             3900..4190
                     /locus_tag="JQG95_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HAY5889572.1"
                     /translation="MGFRFRKRIRIAPGLAINISKSGVSTSIGGKGSTINIGKKGVKM
                     TNGLPGTGLSHTTNLYSPGKSTEKKQLTHKQKIIRNILFVIIVFIIIKAQYF"
     gene            complement(4214..4558)
                     /locus_tag="JQG95_003097"
     CDS             complement(4214..4558)
                     /locus_tag="JQG95_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889573.1"
                     /translation="MRHLLCTVTVFAVLISAPAFADQQCGDFKIHWADDGLARINGAK
                     PEMQKITFLKNKGDYNNIKMDWRMATDQPGRWVGLEYINRNGKAILNAQWLQASMDSP
                     RQYATYDCRKVK"
     gene            complement(4571..5635)
                     /locus_tag="JQG95_003098"
     CDS             complement(4571..5635)
                     /locus_tag="JQG95_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889574.1"
                     /translation="MSLNWMRHFELQLLDQNGQGVSLSDFKVTFQIEWADTRWPRVAN
                     VKIYNLSTDTTNKILGQEFAKIRIIAGYDGIAPDVDASQVGVSREISPDQIGQVNGQN
                     YGLIFDGDIRFTVTGKDNITDSWVLIQAIGDHEAFLYATTITTLAAGYTVADLHRATM
                     QDFNAFGVTQGITGDFPDTVFPRGRAIYSSSRNVMDNIAAQCKATWQLVDGQVQMVPE
                     DKYIHEAIVLNANTGLIGMPQQTMGGGVNVRCLINPNIRINGLIQLDQASVYRATLGN
                     SEIAQSPGRITETEENGNRVLTGTTSQAASIATDGVYIVKAIDYTGDTRGQAWYMDLM
                     CFARGARDLYSPKAMQGTTN"
     gene            complement(5638..5943)
                     /locus_tag="JQG95_003099"
     CDS             complement(5638..5943)
                     /locus_tag="JQG95_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889575.1"
                     /translation="MQFNEIPLSPDNQQFRVLLGNTTYTLRIIWRDAAGWIMDVMDSG
                     GAALLSGVPLLTGVNLLRQYPQLGIDGALVVATDKGAPDEPTKTNLGTYSHLIFVQE"
     gene            complement(5945..6547)
                     /locus_tag="JQG95_003100"
     CDS             complement(5945..6547)
                     /locus_tag="JQG95_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889576.1"
                     /translation="MDILSAIFRQQSRRIGILIPSVVVSEKHSDALEITEHPVEKPTT
                     NSASGFIADHAYKRPSEVTMECGFAGGGSLLDFIDTSSIGLSAGLSPKETYQQLLDLQ
                     SSRVPFDVVTGKRVYSNMLVRAIEVTTDKTSENVLNCTLTLREVIMSQTQSVSVADKS
                     DMQDGVSTSAVQNSGMKSTTPPNESLLSQLGGSVTSAFGG"
     gene            complement(6547..8562)
                     /locus_tag="JQG95_003101"
     CDS             complement(6547..8562)
                     /locus_tag="JQG95_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY5889577.1"
                     /translation="MSGNADTIKDFLVSLGFDIDQAGANKFEAVLKGVTANVLKVGAV
                     VKGAALSIVGFTTQIANGLDKIYWASQRTGASVQGIKALGYAASQTGASAESAMSSLE
                     GLAGFMRSNPGAEGFLNRLGVQTRDASGKMRDTAAIFTGVGQKLNNMPYYRAKQYAQM
                     LGIDENTLMAMRRGMGQLSSEYALTAKRIGFNAESAAKQSNIFMTSMRNLTMTLGQAK
                     DKIGSNLAGGLAGSIDNFRRQILDNWPKIEAVITKIIKGILWAGDAITRVLWRTGQAV
                     EGVIAWFKKLNPATQQLIALFSGLLVAWRLLNTAFMSSPLGMITTLIIALGLLLDDYQ
                     TWKEGGKSLIDWGKWKTEIDQAVKMIGDLKKTVTDLTKALAKLLGIDPKSWSLKWDFS
                     NFISQMGEFGKMLNMIADLLNAIKDGRWADAASIGKQMLNQGSENPSAMPMVTDSANG
                     TADWIKEHWGFDPRSVGRTVRGWFGDDEPEQLGQSVKRPQPTKAGSELLGWMQPMFTN
                     LEQLYRLPEGLLRSVAITESGGNQFAVSGAGAKGLFQFMDGTARDMGLRGNDVFDPEK
                     SAQAAAKYLSQLLRQNGGDLSKALASYNWGIGNVKRYGMGLMPQETRNYIPKVMSNMP
                     TSAPVIQQETNINIHGVFDPREAARLTVDRQKGVNSQLTQQLPAGPR"
     gene            complement(8740..9165)
                     /locus_tag="JQG95_003102"
     CDS             complement(8740..9165)
                     /locus_tag="JQG95_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889578.1"
                     /translation="MEFEIKGVKYRTAKLSVFEQLKVSRKLLPVLAGMVSDFRSVQEK
                     ISSKDTEGAMATILPKIANAVSDLSDGDVDAILFPCLSVVSREHMKGWVPVCQHGEMA
                     FDDIDLLTMLQLVARVVADSLGNFLQGLPTSETPTPLAE"
     gene            complement(9169..9609)
                     /locus_tag="JQG95_003103"
     CDS             complement(9169..9609)
                     /locus_tag="JQG95_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HAY5889579.1"
                     /translation="MATYSFLDVTASLTGPTGVIDLGQGSANSEEGITQTMGGNKNTM
                     TIGADGEVMHSLHADKSGTITVTLLKTSPVNKKLSLAYNAQSQSSATWGNNVIVIRNT
                     ASGDISTARSCAFQKQPDFNNAKEGGTVAWVFDCGKIDQLLGEF"
     gene            complement(9620..10765)
                     /locus_tag="JQG95_003104"
     CDS             complement(9620..10765)
                     /locus_tag="JQG95_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY5889580.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYANDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKIPQTDAGVTRLLSNVEQSMDQSVTNGLVAAGVWNGGPIGQLNSGDTLT
                     KGYYVYAQPLSEQAQADREARKAPLIQVACKLAGAVHYADVQINVVR"
     gene            complement(10769..11326)
                     /locus_tag="JQG95_003105"
     CDS             complement(10769..11326)
                     /locus_tag="JQG95_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889581.1"
                     /translation="MGELRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLFPELINQQWVRRYDMTV
                     RLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(11301..11690)
                     /locus_tag="JQG95_003106"
     CDS             complement(11301..11690)
                     /locus_tag="JQG95_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HAY5889582.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELMPFDGGTPVEQ"
     gene            complement(11677..12231)
                     /locus_tag="JQG95_003107"
     CDS             complement(11677..12231)
                     /locus_tag="JQG95_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889583.1"
                     /translation="MSFKSGVTTRVDNAQAILDALRSLTKKDVLVGIPEEDSEREDVP
                     FGNAGIGYVNEYGSPAQNIPPRPHLIPGVKSVEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRVAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(12228..12635)
                     /locus_tag="JQG95_003108"
     CDS             complement(12228..12635)
                     /locus_tag="JQG95_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY5889584.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKDLFPYFAGLFVAHYMTLWAADSRAMLVGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            12634..12868
                     /locus_tag="JQG95_003109"
                     /pseudo
     CDS             12634..12868
                     /locus_tag="JQG95_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12865..>12993)
                     /locus_tag="JQG95_003110"
     CDS             complement(12865..>12993)
                     /locus_tag="JQG95_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5889585.1"
                     /translation="PLVPLQSVPIQYRGLYQIATYYGKLGAVEPVYKETISYVDGI"
BASE COUNT         2975 a         3612 c         3258 g         3148 t
ORIGIN      
        1 tggacatttg tcgccgatac agtcggctgc cctgacgcgc cagtaacgtt caatgtaact
       61 gtgacagcaa ggactcttga gaatgtcatc gggaacctca tcccgtatgt tgaagataca
      121 ttcggggagc cattcaactg accgcactgt aaaattaatc cagacggaaa tttttgcgca
      181 gcagccgttg cggtattact tgacgcgaac aggctcatat caggtatctg attcgcccct
      241 gtacctacat tccttttagc cgcttctccc aaaccaaggt atgtgagaat gtcagcaata
      301 gtatttttcc caataatgtc acggccaaca gaagttaaat cagtctgcgc tgctgtatca
      361 tttccagtga aatatgggag tttatttgca cctgttgcga gcccagctaa tgccgacagc
      421 gtggcatcaa gcgcctggaa atctttcccg aaagcggtcc ccattttgga tataaacccg
      481 ttcaggtctc catcatcaag cacatccagc ctgcttttgt tggcggtgta ctgcgccaac
      541 gctgccgcga taaagctggc ttgccgaata gctttgttga cctgtgcgct ggatgcttta
      601 cctgccgtaa accctgaaag cagagccgga agtgcttccc agtcagcttg tgaggtgacg
      661 ttagcgttcg gatcaagcgc gaaaggttta aagttgttta ttgccattag agtattgtcc
      721 cccatgctcc aacatcgaac ccgccgatgt attcgttatc catatcaaac ccaaagaatt
      781 tagagccctc ggatggtgct tctaccgaag gcgtttcaac atcaccggcc catacgccag
      841 ctgctttaac ggtgagatag ccctgtttga tagcggcaat cagttcaaga gacacatcag
      901 aaatatcagt ctcggggaat acccaaaccg atatcgtcat gtcctggttg tcgacaattt
      961 gcatcctcag gcctgagcct tcagtcgcag cgtcaaggat gggaggcaga gagtcgttcc
     1021 gaccgtccca gttgttgata gcgattttcg ctttcagaat gatgcggtac gtctcatcgc
     1081 ttagcgtcgt atagccagaa tcaggatcat atggcccttt ccagatgccc tggtcatacc
     1141 caagcccgtc agtgtcccag ctgaaataaa ctccgctaat tggctggctg actatgcgac
     1201 tgcgtccgat ccacagaccg aggatgtcga gctgtacacc gacagcagta tcaatatcga
     1261 aggctgttat aagccctgac atagtgctgg acacatcaat cagcgggcgg gtgctcagat
     1321 ctatatggtc aaaaaagagt ggcttggtag cgtggtagtt agtgatcagt tcggtgtatt
     1381 tgctcatgag gtcaccgtga tactgatatt cgcggtgcta caggacgcag aagcatcata
     1441 ggcaatatca atgtttgatg ccgatacgct gccagacgac ttaccgatca gcaggtcggt
     1501 aatatcgtaa tagcgggcat tcccgccgct cacaacgccg aggtttgccg gggaataaat
     1561 acggctcagc agaacgtcgt cgccaattgt taggccattt atataatcgg caacagcctg
     1621 tttaatctgc tcgccgattt gagaggtata gccggtaaaa actttcaggg taatggctac
     1681 gaaaattggc acatcggtag agcgcgaaaa actgatgacg tgtggattac cgtaagtatc
     1741 cggcaccgtg acagaagttt taccgtaagt tgcggttccc tgccctttac tcccccggat
     1801 tgtttgggct atctcggtaa catcccctcc atcgacgatg gcggaaatag agtgtggcgg
     1861 cagcccgttg ctgtcggttg ccccagtgtc gttctcatat agtttgtgac gtgtcacgcc
     1921 agcaacatta gcgatagcac cgtcgacacc ttcaaacggt gtgatcgatg gtagcgcgac
     1981 gctttgcccc tgccgaatgc gcagctctgc gtcggtttcg gctggtgaac cgacagtagc
     2041 cgcaactgga ttggttaccg acacccaacc tcgggtcggg gtgttgatag tggtaatagt
     2101 cccggccagc gccgcaaccg aaccgctatt cgcacatgtg gccgtcacca gcacagtacc
     2161 atcaacgccg atcgctacac ttgcgggaaa attccagata atgccgtttt tatcccgtgc
     2221 ggagccattc gtgatagtcg tgcctgccgt accggttaac agaaggtcag cagtagagtt
     2281 tgtcgctact tttcgcgtga tcccgttaat tttcacattg ctgctaagcg ctgcggcctg
     2341 cgctgtcgtc ggtgaaaaag agttgtagat cccgatagcg gtgttgttag cgtcatgcac
     2401 ggcaagagcc accagcgcga ccatctgccc gtctttgctg tctggttcga ggtaggcatc
     2461 actaccgtaa atctgcctga aatagctggt cagtgtatct aggattgtct ggtaatcagg
     2521 cgcactaatc ccctgggcgg ttaccgttgc cgatagcccc agcgtgtcga ggttcaaagc
     2581 catttatgcc tcgcttgtta cagtcgtctg gccgtagatt gtgtcaatgg aggaagtgaa
     2641 ggtgacgcga cggctggtgc cgtcataatt ggtatcgaag gaaagaatcg acagaacgcc
     2701 cggtgtatcc tgtatgcgtt cgcgtatagc caggatgtag acatctgatc gctgcttccc
     2761 aagcactgac tgaacatacg gcgtgccttc cgtcagatcg agaaaccact gaccgcgcca
     2821 cagctcgaaa cgggttttta cggcctgggc gacacattcc ggactgtcga taaggaaagt
     2881 atcgtcaccc tggccgaaag tgtaatcgcc ttcagtatct tcgcgacggt atcgcattat
     2941 tgcggccctc cggtagttcc cccgccagtc tgtactccgc catgtttatg cgtggcgaca
     3001 ctgataccag aagctgttac gtcattcgtt accatcaccg gcccaagcat cgtcgcagta
     3061 ccgccgcttt cgcccattcc ctgagacaag ttgccattaa tcgttacgtt gccgttcagc
     3121 gtgatagtcg gggatgtgat tgtcgttcca ccttcagccg tagctgtaag ctggcccggt
     3181 gttttaatgg tgatgttatg tcctgcggcg acttctacaa acgccgcacc atcatcggtt
     3241 cgcagctgcg cggcgctggt actgataccg ctgattttcc gtgcttgcga ctgcgggcca
     3301 acgatggcga acgcatcaga taagtcatgc tggcgcgggt cgacggtctc ctgaacgccg
     3361 ccgctctgcc accaaaaatc gatgcaacgg tcagcaaaaa tcaggaggca ctcgtcgcct
     3421 tcttttaccg gaaaggtcag cgtgcaaccg ccgccgcgcg ggaagatgac cggcacatcc
     3481 accagcggtt ttaattcggt ggatccatcg ccaacaatac cgcgtagcgc cacctctact
     3541 gtgcaggtaa cagtctcagg atcgaacgac tgaatgatgc cgggcatcgc tacgcgcatc
     3601 tgggtagaca ccgaatcggc aatggcctgc gcggtctgct gctcaccgcc gatctgtgat
     3661 tgagttggaa ttggcataaa acccccataa aaaaaccagc cgaagctggt ttggtatcaa
     3721 ttatctctta gtaactaagc tacatcagtg tgaagtaagg cttttccgct gcaatggcac
     3781 acattcacaa ggtgtattag gatatgtttg caacaagtaa gcattgaaaa tcaatttcag
     3841 ccatgaagcc gccatagagt ggacctgcac catcgcatta ttgactacag gagtaacaaa
     3901 tgggatttag atttcgtaaa agaatccgga ttgcgcccgg actcgcgatc aacattagta
     3961 aaagcggagt aagcacttca attggtggta aaggttccac cattaacatc gggaaaaaag
     4021 gcgtaaagat gacaaatggc cttcccggca cggggctgtc gcataccact aacctttact
     4081 ctcccgggaa atcgacagaa aaaaaacaac ttactcataa gcaaaaaata attagaaaca
     4141 tcctgttcgt aattattgtg tttataataa ttaaagctca atatttttga cgcatgcccg
     4201 cctatctggc gggctatttt acttttcggc agtcgtatgt tgcatactga cgcggcgaat
     4261 ccatgctggc ttgcagccac tgtgcgttga gaatggcttt gccgtttcga ttgatgtact
     4321 caagaccaac ccatcttcca ggctgatcgg tagccatacg ccaatccatc ttgatattgt
     4381 tataatcgcc tttgtttttc aggaaggtga ttttttgcat ttctggcttt gcgccattga
     4441 ttctggctaa gccatcatct gcccaatgga ttttaaaatc accacattgc tgatccgcaa
     4501 aagcgggtgc tgatataagc accgcgaaca cggtaacagt gcaaagtaaa tgtctcacgg
     4561 ccccacctca ctaattcgtc gtcccttgca ttgctttcgg gctataaaga tcacgagcgc
     4621 cacgcgcaaa acacatcaaa tccatgtacc acgcctgacc tctggtgtcg ccagtatagt
     4681 cgatagcttt gacgatataa acgccatccg tcgcaatgct ggcagcctgt gacgtcgtgc
     4741 cggtcagcac acggttgccg ttctcttctg tttcggtgat acgcccgggc gactgtgcta
     4801 tttcgctatt gccgagcgtg gcgcggtaca ccgaagcctg atcgagctgg ataagaccat
     4861 taatacggat gtttgggttt atcaggcatc gcacgtttac tccgccaccc atcgtttgtt
     4921 gcggcatacc gatcaggcca gtattggcat tcaacacaat ggcttcgtga atatatttat
     4981 cctccggcac catctggacc tgaccatcca ccagttgcca tgtcgctttg cactgcgcag
     5041 caatattatc catcacgttg cggctggatg agtaaatcgc gcggccacga ggaaacacgg
     5101 tatcaggaaa atcgccggta atgccctgtg tcacgccgaa cgcgttgaaa tcctgcatcg
     5161 tcgcccggtg cagatccgca acggtatatc cagcggcaag cgtggtgatg gtggtcgcat
     5221 agaggaacgc ttcgtgatca ccaatggcct gaatcaacac ccaggaatcc gtaatgttgt
     5281 ccttcccggt gacggtgaag cgaatatcac cgtcaaaaat caggccgtag ttctgaccgt
     5341 tcacctgccc tatctggtct ggtgaaatct cccgggacac accaacctgg ctcgcatcaa
     5401 catccggcgc tataccgtca tacccggcaa tgatgcgaat tttggcaaac tcctgcccaa
     5461 gtatcttgtt cgtggtatcg gtcgaaaggt tgtaaatttt cacgttcgcc acgcgcggcc
     5521 agcgtgtgtc tgcccactcg atctggaacg tgaccttaaa gtcagacagg gaaacgccct
     5581 gcccgttctg gtccaacagt tgcagctcaa aatggcgcat ccagttaaga gacatttcta
     5641 ctcctgtacg aaaatgaggt ggctgtatgt gccgaggttg gttttggtgg gctcgtctgg
     5701 tgcgccctta tcggtcgcca ccaccagcgc gccatcaatg ccaagctgtg gatattgtcg
     5761 taaaaggttt acgccggtca gtagaggtac gccagagaga agcgcggcac cgccgctatc
     5821 catcacgtcc ataatccagc cagccgcatc acgccagatg atcctgagtg tatacgtggt
     5881 attgcccagc aaaactcgga actgctgatt gtcaggagaa agcggtattt cgttaaactg
     5941 catatcatcc cccgaatgct gatgtaacgc ttccgcccag ctggctcagc aaggattcgt
     6001 ttggcggtgt agtggatttc atcccggaat tctgcaccgc cgatgtgctg acgccatcct
     6061 gcatatctga tttatctgca acgctaacgc tctgcgtctg cgacatgatc acttcacgca
     6121 gggtaagcgt gcagttcagc acgttctcgc tggttttatc cgttgtcacc tcgatggctc
     6181 gcaccagcat attgctgtaa acccgctttc cggtcactac atcgaacggc accctagagg
     6241 actggagatc cagtagttgc tgataggtct ctttcgggct aagtccggcg ctgaggccga
     6301 ttgacgatgt atcaatgaag tccagcaacg aaccgccacc tgcgaagccg cattccatcg
     6361 tgacttcgct ggggcgctta tacgcatgat cggcgatgaa acccgacgcg ctattcgttg
     6421 ttggcttctc caccgggtgc tcagtaattt cgagcgcatc agaatgcttt tcagaaacaa
     6481 ccacgctggg gattaatatt ccaattcgcc gggattgctg gcgaaaaatt gctgataaaa
     6541 tatccattat ctcggtcctg cggggagttg ctgggttaac tgtgaattca cgcccttttg
     6601 acggtcaaca gtcaaacggg cagcctcgcg cggatcgaaa acgccgtgga tgttaatatt
     6661 cgtttcctgc tgaatcaccg gggcgctggt gggcatattg ctcattactt tcggaatgta
     6721 gttgcgcgtt tcctgcggca ttagccccat tccataacgc ttaacattcc cgatccccca
     6781 gttatatgat gccagtgctt tgctaaggtc tccgccgttc tgccgcaaca gctggctcag
     6841 gtacttagcg gctgcctgag ctgacttttc cgggtcgaat acatcattcc cacgcaggcc
     6901 catgtcgcgc gccgtgccat ccataaactg aaacagacct ttagcgccag cgcctgaaac
     6961 ggcgaactga ttaccgcccg attccgttat ggccacgctg cgcaataacc cctccggaag
     7021 ccggtagagc tgttccaggt tggtaaacat cggctgcatc catcccagca gttcagagcc
     7081 tgctttggtt ggctgtggcc gcttaactga ctggccgagc tgctcaggct catcatcacc
     7141 gaaccagccg cgcaccgttc ggcccacgct gcgaggatcg aatccccagt gctctttaat
     7201 ccagtcggcg gttccgttgg cgctgtctgt taccatcggc atcgctgacg gattttcgct
     7261 gccctgatta agcatctgtt tgccgatgct ggcggcatca gcccagcggc catctttaat
     7321 ggcgttgagc aggtcggcga tcatgttcag catcttgccg aactcaccca tttgcgaaat
     7381 gaagttgcta aaatcccact ttagggacca tgacttgggg tcaataccga gcaacttagc
     7441 cagcgctttt gtcaggtccg taacagtctt tttcaggtca ccaatcattt tgacggcctg
     7501 atcaatttca gtcttccatt tcccccagtc aatcaggctt ttgccacctt ctttccacgt
     7561 ctggtaatca tccaagagca gaccaagtgc aataataagc gtcgttatca tgcccaaggg
     7621 tgatgacatg aaagcggtat ttagcagccg ccatgcaacc aacagcccac tgaacaatgc
     7681 gataagctgc tgcgtggctg ggttcagctt tttgaaccag gcgatcacac cctcaacagc
     7741 ttgcccagtt cgccataaca cgcgggtaat cgcgtcacct gcccagagaa ttcctttgat
     7801 gatcttcgtg atgaccgctt caatcttcgg ccagttgtcg agtatctgcc tgcggaagtt
     7861 atcaatactg ccagcaaggc caccagctag gttagagcca atcttgtctt tcgcctgtcc
     7921 aagcgtcatc gtcagattac gcatggaggt catgaaaata ttggactgtt tagccgctga
     7981 ctcagcatta aaaccaatac gctttgccgt caacgcgtac tcagaactga gctgccccat
     8041 ccctctgcgc atcgccatca gcgtgttttc atcgatgccg agcatctgcg cgtattgctt
     8101 cgcgcggtaa tacggcatgt tgttgagctt ttggccaacg ccagtaaaga tggcagcagt
     8161 atcacgcatc tttccgctgg catcgcgggt ctggacacca agacggttca ggaacccttc
     8221 cgcccccgga ttgctacgca tgaagccagc caacccttcg agggaggaca tagccgactc
     8281 ggcgctggca ccggtttgcg atgcggcgta tcccagcgct ttgatgccct ggacactggc
     8341 ccccgtccgc tgggatgccc agtaaatttt atccagaccg ttcgcgatct gagtggtaaa
     8401 tccgacaatg ctcagcgctg cgcctttcac caccgcgccg accttcagaa cgttcgcggt
     8461 aacgcctttc agcacggctt caaacttatt agcgccagcc tgatcgatat cgaatcccag
     8521 cgaaacaagg aaatctttaa tcgtatctgc gttaccgctc attggccgct ctccatttat
     8581 ctacccgggc gtcgttatcc tcgcgcatgt cgaggtagtc attgagaagc gcgatgcggc
     8641 aaagatctac cgcaccgctg ttaaggtctt tctggtcaat atggaaggca agcgccgggc
     8701 gaagaataaa atcttcaccg cccggcaggc tgttgaaggt tattccgcta gcggggtggg
     8761 cgtctcgctg gtagggagtc cttgcaaaaa atttcccagc gagtcggcga ccacccgcgc
     8821 caccagttgc agcatggtaa gcaggtcgat atcgtcaaac gccatttcgc catgctggca
     8881 gaccggcacc cagcctttca tgtgctcgcg tgaaacaacg gaaagacagg ggaacaggat
     8941 agcgtccacg tcgccatcac tcagatcgga cacagcattg gcaatctttg gcaggatagt
     9001 agccatcgcg ccttcggtgt ctttgctgct gatcttctcc tgaacgctcc ggaagtccga
     9061 aaccatcccg gccagaaccg gcaacagctt gcgggacacc ttcagctgtt cgaaaacgct
     9121 gagctttgcg gtgcgatatt tcacgccttt aatttcgaat tccatgcgtt aaaactcccc
     9181 gagaagctgg tcaatcttgc cgcagtcgaa tacccaggcg acggttccgc cctctttagc
     9241 gttattgaaa tcaggctgtt tctggaatgc acacgaacgc gcagtagaaa tatcacccga
     9301 tgccgtgttg cgaatgacga tcacgttatt gccccaggtg gcagaggact ggctttgcgc
     9361 gttatacgcc agagacagct ttttattcac cggggaggtt ttgagtagcg tcaccgtaat
     9421 ggtgcctgac ttatcggcgt gcaggctgtg catcacctcg ccatcggcac cgatagtcat
     9481 ggtgtttttg ttgccgccca tggtctgggt gataccttcc tcagagttcg cagaaccctg
     9541 accaagatcg ataacgccgg tcggcccggt gagcgacgcg gttacatcga gaaaagaata
     9601 agttgccatt tatcgctcct tagcgaacca cgttgatctg cacatcggca taatgaactg
     9661 cgccagccag cttacaggcc acctggatta acggtgcttt gcgcgcttca cggtcggcct
     9721 gcgcctgttc ggacagcggt tgcgcataca cgtaatagcc tttggtcagc gtatcgccgg
     9781 aattcagctg tccgataggg ccaccattcc acacgccagc agctaccaga ccgttcgtga
     9841 cggactgatc catggactgt tcaacgttgg aaagcagacg ggtcacaccg gcatcagtct
     9901 gcggaatttt ggtggtgctg gtgtaaagca ggttatagag gttggtctga acgtagttct
     9961 gcaaccagtc gagcccgtgg cgctcgtcga agaagtcacc gttcgccatg acaccctgtt
    10021 gcaggatcgc cgtgtcgttg gcgtagtaca cgaatacatt gcagtttttt gcatcaagtg
    10081 ccgatgcctg gctgactgtc agtgtttcat acccgacacc cggctcctgc ttaaacttga
    10141 gcgtaatcgc ggtattactg ccattgaaat taaccgtgaa tgcccggcca aatgcagata
    10201 acgcagcgta tttattaccc gatgaatatt gaataaaact gcgtgaatat ccggcggttt
    10261 tcagttttga tgccaaatca tcgctggatg cagtctgcag gcatttctca tcgcttgtcg
    10321 taatcgccag aatacggctt acagaagagg attcgatcgc cgcagccact ttcagccagt
    10381 ctgcatccgg aatatcttca tcgtctgcaa tccccagccc ataccatgaa gtataatcga
    10441 gcatggcatt cacagcctgc tccagcgtct caggcgtggc ctgttcgctg tctcccttcg
    10501 ttttcaccca acgaccaaca aaaacctcct gaggtttcgg tgattgtgag aaaaacacct
    10561 gcgcagcctt atattctggt gattccacgc caaaatcttt tccaatatct tccgcggcag
    10621 aataacggcg aatgcgctca cttaccggaa tgattgtgga cgggccgaga atgagtaatg
    10681 caccaaaatt tcgccctgat gctgcacgcg gcgacatgat cacatcaaca ttaacaacgt
    10741 ttgatacagg caagccctgt gccatagctt aatctccgaa aaagatgact ggtgcttcca
    10801 ccagcgattt aataccgtac tcgcgcacaa ccttccggcg cagacgcacc gtcatatcgt
    10861 agcggcggac ccattgctga ttaataagtt cagggaagag acctgtgtaa tcgccaagag
    10921 acagccccag cgcattcagt gctgcgttgt tctgcggtac agatataccg tcacgaaacc
    10981 gggacgcata caccatcccc gccggaccat aaaacgaagc catacactca atcgtttcat
    11041 gccgccagag ctgagagcca tcatcggtct gtctggtgaa tgccggactg tcatcacctg
    11101 accatccgat aaccccaaac gcacaccagt tcgtttcaac cggtagcagt ggcggctgct
    11161 ctttctgcca gcgcgggcga accatcccgg cagacagacc ggaaacgtta cgcatccact
    11221 ggcttaacag cctgtcgagc gcttcgtcat aatccggatc gccactggtt ggtattaacc
    11281 atccgcgctc tgtactggtg ttattgctca accggagttc ccccatcaaa cggcatcaac
    11341 tcacaatgcg cctgaacgaa tccggcccca taagctgtat acgggtcgac gaaggtcaca
    11401 cgataatcac ggccctgata cgtcacgata tcggcatcac ggccagtctg tccctgcgtc
    11461 agtcgctcag tcgtcacaat cagaattgca ccgctgatta cctgccctgc ctgcatacgg
    11521 cggttttcca gagagcgatc aacagttacg actccggcaa actgcttttt aacttcactg
    11581 tcgctgccga tcccgtcctc atccaccgtt tgcactcggc gtgttaccca caaattgaag
    11641 tcgcaaaaat cggggtcaaa aagcacatct gttacatcaa gagtcggcat ctttatccct
    11701 cacaacatgg gtaatagctc tgcgatattg cccggtgtca attaatggtt tcgccagatc
    11761 ggttccggga gattcgccag caacacgccg agcaagttcc agtgttgccc ccttgcgccc
    11821 ccgacgagcc cgggcttcaa cggtgctatc agcaagcggt gtaaagccgg taatggtcat
    11881 gtaacgcctg acgccattcg cggccagcgt tccggcgcgg ttaagcgctc tttccgcacc
    11941 cgccgcattt ccatcaagcg cagcctgcgc cgctgctttg agctgcggca ccgtctgttc
    12001 ctctacggat ttaacgccgg ggatcaggtg cgggcgtggg ggtatgtttt gcgctggtga
    12061 gccgtattcg ttgacgtaac cgatcccggc attaccaaac ggaacatcct cacgctcgct
    12121 gtcttcttcc gggatgccca ccagcacatc ctttttggtt agcgaccgga gcgcatccag
    12181 aatggcctga gcgttatcaa ccctcgttgt tacaccactt ttgaaactca tagctggcga
    12241 ccgcccgcac cgaacatcgt gatcagctga taaaattcag cgccatatcg ggtgttattc
    12301 cagaagcctg cgtcagggtt tagcgtcgcg ctggtgtcat agctgacgct taccttgtca
    12361 acggacttgg aggactgaac accattggtt gaaccgcccg ggccgccaac cagcattgcc
    12421 cgactatctg ccgcccatag cgtcatgtag tgagccacga acaacccggc aaagtacgga
    12481 aacaaatctt tgccggtgac gttttcgctc agcagttcat cggccagatt cagacgaaac
    12541 tggatttgcg cttcgggata tttggcaggg tcagcaaact gcgggaagtc gcggcgaaaa
    12601 tcacttaccg ctggcaggct ttgattcttt ggcatttttt acctcgttac gcgcgtctgt
    12661 ggctttgcca acggatactt ccgcgtgcgc acgagtgaac cagtgcgtgg caacgtcttc
    12721 ctccactgca tgacggcctt taacaaactc gcgccgcgaa ccgtcgggaa gcgtgagcac
    12781 aaacggggta tgtacgtgta ttactgcatt attttttgcc atcgggtcat ccttaatggc
    12841 cccgccaggg gggccatgtg gctgttaaat gccatcaacg tacgaaatgg tttctttgta
    12901 cactggctca accgcaccca gcttgccgta gtaggtcgca atctgataca gaccgcgata
    12961 ctggatagga acgctctgca acggcaccag cgg
//
LOCUS       DADLCQ010000112        12908 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000112 DADLCQ010000000
VERSION     DADLCQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.112"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(128..1579)
                     /locus_tag="JQG95_003111"
     CDS             complement(128..1579)
                     /locus_tag="JQG95_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY5889586.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="JQG95_003112"
     CDS             complement(1806..3224)
                     /locus_tag="JQG95_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY5889587.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="JQG95_003113"
     CDS             complement(3378..3722)
                     /locus_tag="JQG95_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY5889588.1"
                     /translation="MQYLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JQG95_003114"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="JQG95_003114"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY5889589.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="JQG95_003115"
     CDS             complement(4712..5647)
                     /locus_tag="JQG95_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY5889590.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="JQG95_003116"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="JQG95_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY5889591.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="JQG95_003117"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="JQG95_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY5889592.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JQG95_003118"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="JQG95_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY5889593.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="JQG95_003119"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="JQG95_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY5889594.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAEICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="JQG95_003120"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="JQG95_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY5889595.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="JQG95_003121"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="JQG95_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY5889596.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JQG95_003122"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="JQG95_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY5889597.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLIFDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="JQG95_003123"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="JQG95_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY5889598.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="JQG95_003124"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="JQG95_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY5889599.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3296 c         2950 g         3260 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aagggatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 agtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagatactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcgga aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagggcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccaag
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgaaaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctact
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DADLCQ010000113        12890 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000113 DADLCQ010000000
VERSION     DADLCQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.113"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..195)
                     /locus_tag="JQG95_003125"
     CDS             complement(<1..195)
                     /locus_tag="JQG95_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY5889600.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKD"
     gene            complement(401..1120)
                     /locus_tag="JQG95_003126"
                     /pseudo
     CDS             complement(401..1120)
                     /locus_tag="JQG95_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="with CusA, CusC and CusF is part of a cation efflux
                     system that mediates resistance to copper and silver;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1136..1468)
                     /gene="cusF"
                     /locus_tag="JQG95_003127"
     CDS             complement(1136..1468)
                     /gene="cusF"
                     /locus_tag="JQG95_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY5889601.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1492..2874)
                     /gene="cusC"
                     /locus_tag="JQG95_003128"
                     /pseudo
     CDS             complement(1492..2874)
                     /gene="cusC"
                     /locus_tag="JQG95_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            3031..3714
                     /gene="cusR"
                     /locus_tag="JQG95_003129"
     CDS             3031..3714
                     /gene="cusR"
                     /locus_tag="JQG95_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY5889602.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTLSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3704..5146
                     /gene="cusS"
                     /locus_tag="JQG95_003130"
     CDS             3704..5146
                     /gene="cusS"
                     /locus_tag="JQG95_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY5889603.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNQLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5296..7533
                     /gene="nrfB"
                     /locus_tag="JQG95_003131"
                     /pseudo
     CDS             5296..7533
                     /gene="nrfB"
                     /locus_tag="JQG95_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            7520..9664
                     /locus_tag="JQG95_003132"
                     /pseudo
     CDS             7520..9664
                     /locus_tag="JQG95_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741327.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage receptor"
     gene            9781..10488
                     /locus_tag="JQG95_003133"
     CDS             9781..10488
                     /locus_tag="JQG95_003133"
                     /inference="COORDINATES: protein motif:HMM:NF024679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage N4 adsorption protein A"
                     /protein_id="HAY5889604.1"
                     /translation="MSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVYS
                     RVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRAS
                     ASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQTI
                     EPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAIN
                     QRNGERNNAFLTIGVHW"
     gene            10489..11379
                     /locus_tag="JQG95_003134"
     CDS             10489..11379
                     /locus_tag="JQG95_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY5889605.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11562..12323
                     /locus_tag="JQG95_003135"
     CDS             11562..12323
                     /locus_tag="JQG95_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5889606.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQREKVMW"
     gene            complement(12369..12445)
                     /locus_tag="JQG95_003136"
     tRNA            complement(12369..12445)
                     /locus_tag="JQG95_003136"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12409..12411),aa:Arg,seq:tct)
     gene            12688..>12890
                     /locus_tag="JQG95_003137"
     CDS             12688..>12890
                     /locus_tag="JQG95_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY5889607.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3155 a         3342 c         3274 g         3119 t
ORIGIN      
        1 atccttcaaa gacagggatg ggtatcgacc aagcccaagt cgattaggct tgccatgcca
       61 gcgatagcgg tactggaact ggatgacccc cttcggtgaa attcgtacgc tgaggccatc
      121 agcatcagcc acttcttgtg ggccagaata tggtttacca taaatagtac gcagttttgt
      181 gtcgcttata gccataaata atatttggta cgctcagaaa taatggtttg gtacacatct
      241 ggtacacaat atcacatgga caacagcgca cagtaaacaa ctatatttga cgttcgtaga
      301 catactcagg tgaaaaaagg gttgtttttc gagttataac agacagttaa ttaactacag
      361 tggacaatgc tagacaatga cagacacaga taagcaacct accttcctct ttcgcgacca
      421 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
      481 tgggcgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg tggcgaatat
      541 ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg
      601 taccgccggt ttcgcgcaac agtaaatact cactctgcgc ttccacccag tcgggaatgg
      661 tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct
      721 tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga
      781 cattcgccgg gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca
      841 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat
      901 cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc
      961 gcgtattggg atacatcggg tcgtaccaga ataagatttt acgttctgcg gtcggcattt
     1021 tttctgtgtg cggttccgcc tggtcaaacc aggtaaaacc tgccgctgaa ataataccgc
     1081 ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact
     1141 ggctgacttt aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca
     1201 ctttgtcgcc ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatgg
     1261 tcatctccgg ccagttgacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt
     1321 ccagatcaac accttttacc acaccggtgg cactaatgac ctgtggttgt gcttcgctca
     1381 tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga
     1441 acattgcgac ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg
     1501 ccaaccgccc cctaaggcgg tatacaaata aatttcgtta acctgacggg cataattcag
     1561 gtcaagtaaa gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata
     1621 acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc agggtaattt gcagcgacgc
     1681 cagataacgc tgctgggcgc tgatttgatc gtccaggctt tgacgtaatg ccagcgcatc
     1741 tgccacctct ttaaaggcgt tctggatttt ctgttcataa ttcaccaccg actgctgctg
     1801 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
     1861 aattttagga ataaaattcc acatcccgct gctagcgtta aataatgacg acagatcgct
     1921 gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc
     1981 accaatatta gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg
     2041 caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgccgctgtt
     2101 tactgtctgc gcttgcggta gcttgccgta gcttcccaat aacagttgca atgcattatt
     2161 cgcctgcgcc agttccccct gacgtttagc gatgtcactg cgggtacttt ctatcacccc
     2221 gcgagcctgt tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa
     2281 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
     2341 cagctgctga ttgaaatagc tttgcgcgac attagaaacc agcaaaatat gcaccgcgcg
     2401 ctgggcttcc tcagtggcta aataattttg gcgctcggct tcgctcatgt tctttaagcg
     2461 accgaaaaaa tcgagatcaa agctggcgtt aaggccagtc gagaactccc gcgtcgtggc
     2521 tgaatcgcct ttaagattgc cgctccagct gccgctgccc tagccattga gctgtgggta
     2581 gcggtcggca tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat
     2641 gcgcaaatcc cggttattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac
     2701 aaaaaaggtg cgccagccca cgttctggta gttatccgct gcgttaacca ggccgttctg
     2761 gctgagtgag aactgctgcg gtacgggcat taccggacgc tgataatccg gtgccagtga
     2821 acaaccagtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
     2881 ataatttctg gtgattttat gtcgccaact ttactcgcca ggctctgatt ttccggtgac
     2941 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
     3001 agaattgccc gggcaacatg cggaggaaat atgaaactgt tgattgtcga agatgaaaag
     3061 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
     3121 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgacct gataatcctc
     3181 gatattatgc tgccagacgt gaacggctgg gatatcgtgc gcatgttgcg ctccgccaat
     3241 aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
     3301 ctggagttgg gggcagatga ctacctggtg aaaccgttcg cttttgctga actgctggcg
     3361 cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
     3421 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc tcagcggcac gcgcatcact
     3481 ctgaccagta aagaatttac tctgctggag ttttttctcc gccatcaggg cgaagtgctg
     3541 ccccgctcgc ttatcgcctc gcaggtatgg gacatgaatt tcgacagcga cactaacgcc
     3601 attgatgtgg cggtgaagcg gctacgcggc aaaatcgaca acgactttga gccgaagctg
     3661 attcaaaccg tgcgcggcgt gggttacatg cttgaggtgc cggatggtca gtaagccatt
     3721 tcagcgcccg ttttcgctgg caacccgcct gacctttttt atcagcctgg ccaccatcgc
     3781 ggcgtttttc gcctttgcat ggatcataat ccactcagta aaagtgcatt ttgccgagca
     3841 ggacattaat gatttaaaag agattagcgc cacccttgaa cgggtactaa atcaccctga
     3901 cgaaacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt
     3961 gttgatttcc ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgcctga
     4021 tatccgcgag tttgcacgtg atgccatacc cgataaagac gcgcggggtg gcgaggtgta
     4081 tctcctttcc ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag
     4141 caactggcgg atgattaact tgccggttgg cccgttggtg gacggcaaac cgatttatac
     4201 gctctacatc gcgctgtcga tcgattttca tcttcattac ataaatgatt tgatgaataa
     4261 acttattatg accgcatcgg taatcagcat cctgatcgtc tttatcgtgc tgttggcggt
     4321 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
     4381 agatctcgac gttcgccttg acccgcagac cgtgccgatt gagctggaac agctggtact
     4441 gtcgttcaac catatgatcg agcgtattga ggatgtcttt actcgccagt ccaatttctc
     4501 agcggatatc gcccacgaaa ttcgcacacc aattactaat ctcattacgc aaacggaaat
     4561 cgccctcagc cagtcgcgca gccagaagga gctggaagat gtgctctact ctaatctcga
     4621 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa
     4681 caaccagcta atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt
     4741 cgattttttc gaggcgctag cggaagatcg cggcgtggag ttgcggtttg ttggcgacaa
     4801 acgtcaggtt gcgggcgatc cgttgatgct gcgtcgggcg ctaagcaacc agctttctaa
     4861 tgcccttcgt tatacgccaa ccagagagac aattgtagta cgctgtcaga cggtcgatca
     4921 ccaggtgcaa gtttccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg
     4981 attgtttgac cgtttctatc gcgttgaccc ctcccgccag cgaaaaggtg aaggtagcgg
     5041 tattgggctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcggtaac
     5101 gtcagatgcg cgggggacaa ggtttgttat cacattaccc gcttaattat tcagcaaacg
     5161 gcaactttta taaccagtgt aaaaataacg tgccgcaata gtcatacata ttaatggtaa
     5221 aaagctgtca caattcataa aaaaccttaa tatacgccac cctaaacata accagcgtta
     5281 atgtaaggtt tttgtgtgga ctggcttctt gatgtttttg ctacctggct ctacggctta
     5341 aaagtaatcg cgataacgtt agcggtcatc atgttcatca gcgggctgga cgattttttt
     5401 attgatgtcg tctactgggt acgccgcatt aaacgcaagt tgagtgttta tcgccgctac
     5461 ccgcgaatga gttaccgcga actgtataaa ccagatgaaa aaccgttagc gattatggtt
     5521 ccggcgtgga atgaaacggg cgtcatcggc aatatggccg agctggcggc gaccacgctc
     5581 gactacgaaa actatcatat ctttgttggc acctacccca acgaccccga tactcaacgt
     5641 gatgttgacg aagtgtgcgc tcgctttccg aacgtgcata aggtagtctg cgcgcgtcct
     5701 ggtcccacca gcaaagccga ctgcctgaac aacgtgctgg acgccatcac ccagtttgaa
     5761 cgtagcgcca atttcgcttt tgctggtttt attctgcatg acgccgaaga tgtgatttca
     5821 ccgatggaat tgcgtctgtt caactatctg gtcgagcgta aagatctgat tcagatccca
     5881 gtgtatccgt ttgaacgcga atggacgcac ttcaccagca tgacttacat tgatgagttt
     5941 tcagaactgc atggcaaaga tgttccggtg cgtgaagccc tcgccggaca agtgcccagc
     6001 gcaggcgtcg gcacctgttt cagccgccgc gccgtgaccg cactgttagc tgacggtaac
     6061 ggtattgctt tcgacgtgca gagtcttact gaagattacg acattggctt ccgcctgaaa
     6121 gaaaaaggta tgacggaaat ttttgtccgt tttccggtgg tggacgaagc caaagaacgc
     6181 gagcagcgta aatttttaca gcacgcacgg acgtcaaaca tgatctgcgt gcgcgaatat
     6241 ttccccgata ccttttcgac tgcggttcga caaaaatctc gctggatcat cggcattgtt
     6301 ttccagggct ttaaaaccca taaatggacc tccagcctga cgctgaacta ctttctctgg
     6361 cgcgaccgca aaggggcaat cagtaacttt gtcagcttcc tcgcgatgct ggtgatgatc
     6421 cagcttttgc tgttgctggc gtatgaaagt ttgtggcccg atgcctagca tttcctttct
     6481 atttttagcg gcagcgcatg gttaatgacc ctgctgtggc taaactttgg cttgatggtt
     6541 aaccgcatcg tgcagcgagt gattttcgtc actggctact acggcctcac gcagggcctg
     6601 ctttccgtcc tgcgtctttt ctggggcaac ctgattaact ttatggctaa ctggcgcgtg
     6661 ctgaaacagg ttcttcagca cggcgatccg cgtcgcgtcg cgtgggataa aacaacgcat
     6721 gacttcccca gcgtcactgg cgatacccgc tcgttgcgcc cgttaggtca aattctgctg
     6781 gaaaatcagg tcatcactga agaacaactc gatacagcac tgcgtaatcg cgtcgaaggt
     6841 ctacgcctgg gcggttcaat gctgatgcag gggctgatta gcgccgagca gctggcacag
     6901 gcgctggcag agcaaaacgg cgtggcgtgg gaatccatcg atgcctggca gatcccgtcc
     6961 tcgctgattg ccgaaatgcc agcctccgta gcgctgcatt atgcagtact gccgctgcgt
     7021 ctggaaaatg atgagttaat tgtcggcagt gaagatggta ttgacccggt ttcgctggcg
     7081 gccctgacgc gtaaagtcgg acgcaaagtg cgttacgtca ttgttctgcg gggacaaatt
     7141 gtcacagggt tacgccactg gtatgcacgc cgacgcggtc acgatccgcg ggcaatgttg
     7201 tacaatgcgg ttcagcatca gtggctcacg gaacagcaga ccggtgaaat ctggcggcaa
     7261 tatgtgccgc atcagttcct gttcgccgaa atactgacca cgctcggtca tattaatcgt
     7321 tcagcaatta acgtgttgtt attgcgccat gaacgcagtt ctctgccgct cggcaagttt
     7381 ttggtcaccg aaggcgttat cagccaggaa acgttggatc gcgtcctgac aattcaacgc
     7441 gaattacaag tttcgatgca atcactatta ctcaaagcag gtttaaacac agaacaggtt
     7501 gcgcaactgg agtccgaaaa tgaaggagaa taaccttaat cgcgtcatcg gatggtctgg
     7561 tttactgctg acgtctttat tgagtaccag cgcactcgca gacaatatcg gcaccagcgc
     7621 agaagagctg gggctgagcg attatcgcca ttttgttatt tatccccgtc tcgataaggc
     7681 gctgaaggca cagaaaaata acgacgaagc aaccgccatc cgcgaatttg aatatataca
     7741 ccaacaggtg ccggataata ttccgctgac tttatacctt gcggaagcct atcgtcattt
     7801 tggtcatgat gaccgggcgc ggctgttgct tgaggatcaa ctgaaacgtc acccaggaga
     7861 tgcccgactt gagcgcagtc tggcggctat tccagttgaa gtgaaaagcg ttacgactgt
     7921 tgaagaactg cttgcccagc aaaaagcggg cgacgctgcg ccgaccctgc gttgtcgcag
     7981 tgaagtcggg cagaataccc tgcggctggc acaattacct gtcgccagag cgcaactgaa
     8041 cgatgcgacg tttgctgcat cgccggaagg aaaaacgctg cgaaccgatc tgctgcaacg
     8101 ggcaatctac ctgaaacaat gatcccaggc agatacgcta tacaatgaag cacgccagca
     8161 gaacacatta agcgcggcag aacgccgtca gtggtttgac gtgcttcttg ccgggcagct
     8221 ggacgatcgg atcctggcac tgcaatcaca ggggatcttc actgatcctc agtcatatat
     8281 tacttacgcg accgcgctgg cttatcgtgg cgaaaaagca cgcctccagc attatctcat
     8341 tgaaaataag ccgctgttta ccacgggcgc acaagagaaa agttggctct atctgttatc
     8401 taaatacagc gccaaccccg ttcaggcatt ggcgaattat acggtgcagt ttgccgataa
     8461 ccgccagtat gttgttggcg cgacgctacc ggtgctgtta aaagaaggtc agtacgacgc
     8521 agcgcaaaaa ctgctcgcca ccctccccgc caatgaaatg cttgaggagc gttatgctgt
     8581 cagcgtggcg acccgtaaca aggctgaatc tctgcgtctg gcacgattgc tgtatcagca
     8641 agaaccgaca aatcttcccc gcctggatca actgacctgg caactgatgc agaacgagca
     8701 gtcacgcgaa gctgccgatt tattgctgca acgctatcct ttccagggcg atgcgcgtgt
     8761 cagccagact ttaatggcgc gactggcgtc tctgctggaa agtcatcctt acctagcaac
     8821 gccggcgaag gtggcgattt tatcgaaacc cttaccgctg gcggagcaac gtcagtggca
     8881 aagtcagttg ccgggtattg cagataattg cccggcaata gttcgcttgc tgggcgatat
     8941 gtcgccttcc tacgatgctg ccgcctggaa ccgtctggca aagtgttatc gggacacgct
     9001 acccggtgtg gcgttgtatg catggcttca ggccgaacaa cgacaaccga gcgcctggca
     9061 acatcgtgcg gtagcctatc aggcgtatca ggttgaggac tacgccaccg cactggcggc
     9121 ctggcagaaa atcagtcttc acgacatgag taatgatgat ctgctcgccg ccgccaacac
     9181 cgctcaggca gcgggaaatg gcgcggctcg cgatcgctgg cttcaacagg cagaacaacg
     9241 tgagctggga aacaatgctc aatactggtg gctgcatgcg cgacgttata tcccagctca
     9301 gccagaactc gcactgaacg atctcacgcg ctcaatcaat attgcccctt ctgccaactc
     9361 ttacgttgcg cgggcgacaa tttatcgcca acgtcataat gttccggccg cggtgagtga
     9421 tttgcgcgcc gcgctggaac tggaaccaaa taatagcaac acccaggcag cgcttggtta
     9481 cgccttgtgg gatagcggtg atatcgcaca gtcgcgggaa atgctcgaac aggcgcataa
     9541 agggcttccg gacgatccgg cactgatccg acaactggcc tacgtgaacc agcgtctgga
     9601 tgacatgcct gcgacgcagc actacgcccg gctcgtgatt gatgacattg ataatcaggc
     9661 ataaccccac tgaccccaga gcaaaatcag caacgcttca atttccgccg tttgcatgag
     9721 gaggtcggtc gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggcgca
     9781 atgagtaccg ctaacaataa tgtcggcggt gcagcgcctg ggaaaagcta tcgtagctac
     9841 ggacaactgg aagccgagta ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc
     9901 tcagtttata gccgcgtctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa
     9961 aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc
    10021 gccgtcgaac agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc
    10081 gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca
    10141 ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag
    10201 gcgtggacgg cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt
    10261 gagccttacg ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag
    10321 cttggcggtg tcggggtccg ctggaatatt tggaccggcg agacgcacta cgacgcctgg
    10381 ccgcacaaag tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt
    10441 aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc
    10501 attttcgtat tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt
    10561 atctggcaac cacaaaaccg agatagtcag gttaccgata cccagtggca ggggctgatg
    10621 agtcagttac gttttcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat
    10681 gcatttaccc agccagaaca gcgcacgtta ttgtttaagc gtgccgcagc tgcgcaacag
    10741 actggcctga agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa
    10801 cagtcgtctg cagcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa
    10861 gccagattat ggagcgccgc gcctggcata acgccggatg gctggtacat cagcgcggaa
    10921 attgacgacc tgaactggcg cagcgaagcc gcccgtcaac ctttgctaac atggttaaac
    10981 aacgcgcagc ggctgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc
    11041 gccggaaaca tgtcgcccga tggctatcgc caactgctgg aacaagttaa agcaaccgga
    11101 gttaatgtct gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa
    11161 cgttatctac aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat
    11221 gaactgtttg tcgccggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa
    11281 attgcctcgc tgttagcgaa acgttcctct tgcggtaaag acatgctcta tttctctctg
    11341 cgctatttgc ccgtcgcgca cggcattctc gagtattaaa tcccctccag gtaagtcggg
    11401 tacgacctgg cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa
    11461 gcatgctaaa gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat
    11521 agatcctttg agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg
    11581 aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta
    11641 cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac
    11701 tctcaacgtt aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc
    11761 tgttgttttt aaataagaaa ttaacctgtg taaagccctg gtcgcggctg gcaaccccgg
    11821 ttatcgcttg tcatagccgt acaccggaag tgttccggct agccaccaac cacagcaagc
    11881 agcaatccag caaaccctgc gaagcggagt tgacgcgcgc attacttttt accgtattgt
    11941 ctaactttct tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg
    12001 tccgcgacag cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa
    12061 ttgtcgccag ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa
    12121 ataccagcta tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat
    12181 tgatggataa caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt
    12241 actttatctc tgtttttaag gcgttttaca gcctgacacc gttgaattat ctcgccaaac
    12301 agcgagaaaa agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaatga
    12361 agaagaaatg gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg
    12421 ctctatccaa ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta
    12481 tacggtcaac ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc
    12541 gcgtctgatt ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc
    12601 tgaatcaaga aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa
    12661 ttgtccgctc ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact
    12721 catcctatca tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt
    12781 gtcctgaaaa cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat
    12841 ttaatcatta tggatataga cttgcccgga acagacggtt ttaccttcct
//
LOCUS       DADLCQ010000114        12695 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000114 DADLCQ010000000
VERSION     DADLCQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.114"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQG95_003138"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="JQG95_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY5889608.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQT
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="JQG95_003139"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="JQG95_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY5889609.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="JQG95_003140"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="JQG95_003140"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY5889610.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="JQG95_003141"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="JQG95_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY5889611.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="JQG95_003142"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="JQG95_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY5889612.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="JQG95_003143"
     CDS             4025..4615
                     /locus_tag="JQG95_003143"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY5889613.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="JQG95_003144"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="JQG95_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY5889614.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="JQG95_003145"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="JQG95_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY5889615.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="JQG95_003146"
     CDS             complement(6551..6658)
                     /locus_tag="JQG95_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889616.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="JQG95_003147"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="JQG95_003147"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY5889617.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="JQG95_003148"
     CDS             7648..7875
                     /locus_tag="JQG95_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889618.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQG95_003149"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="JQG95_003149"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY5889619.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="JQG95_003150"
     CDS             complement(9447..10703)
                     /locus_tag="JQG95_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY5889620.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSEPITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSVQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="JQG95_003151"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="JQG95_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY5889621.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="JQG95_003152"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="JQG95_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY5889622.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQG95_003153"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="JQG95_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY5889623.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3034 a         3245 c         3197 g         3219 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcggtc tgctccagtc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt aacgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggacac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 ggctctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcagtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DADLCQ010000115        12683 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000115 DADLCQ010000000
VERSION     DADLCQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.115"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            273..530
                     /locus_tag="JQG95_003154"
     CDS             273..530
                     /locus_tag="JQG95_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY5889624.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            766..840
                     /locus_tag="JQG95_003155"
     CDS             766..840
                     /locus_tag="JQG95_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY5889625.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
     gene            833..1690
                     /gene="repA"
                     /locus_tag="JQG95_003156"
     CDS             833..1690
                     /gene="repA"
                     /locus_tag="JQG95_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY5889626.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPRKGAETLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2392..2652
                     /locus_tag="JQG95_003157"
     CDS             2392..2652
                     /locus_tag="JQG95_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889627.1"
                     /translation="MAENGYGLAGQGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISICWWLCLKGYILCIKPDNTQIARQMSIMHPVIACL"
     gene            2759..3022
                     /locus_tag="JQG95_003158"
     CDS             2759..3022
                     /locus_tag="JQG95_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5889628.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSAQLRQQLQGRYQELRNEL"
     gene            3012..3311
                     /locus_tag="JQG95_003159"
     CDS             3012..3311
                     /locus_tag="JQG95_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY5889629.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLVENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFIL"
     gene            complement(3347..4012)
                     /locus_tag="JQG95_003160"
     CDS             complement(3347..4012)
                     /locus_tag="JQG95_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889630.1"
                     /translation="MMFWNRNKKDKATIANEKKRFNHLLSVAEKLPVKALPDLIRAIV
                     RPVQSDFLLAVAEEGTDARPDMTPQEFFFKGLTQIQSYEKMKECEMNGADYPLSLASD
                     IVLPWPWSLPRYIDNVSRIGTHKGKPWKQDKNNHYVDLWLPWRIGFVHGGNHSITAGI
                     LAGEGSLIPEHVYDMSWMFELVRTDGNNWFVEGQKAEAVKSGRSAAVFEIGRLLVEGA
                     KER"
     gene            complement(4582..5145)
                     /locus_tag="JQG95_003161"
     CDS             complement(4582..5145)
                     /locus_tag="JQG95_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY5889631.1"
                     /translation="MDIIEKRITKRHLSESELSGVNYYNCIFERIQLDNFNFRDCEFE
                     KCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGVNAADIMFPCTFSLVNCDLRFVDFISL
                     RLQKSIFLSSRFRDCLFEETDLRKSDFTGSEFNNTEFRHSDLSHCDFSMTEGLDINPE
                     INRILSIKIPQEAGLKILKRMGVVVGG"
     gene            complement(5149..5880)
                     /locus_tag="JQG95_003162"
     CDS             complement(5149..5880)
                     /locus_tag="JQG95_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5889632.1"
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGA
                     GKTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLT
                     KKQAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDE
                     PTVGIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDM
                     SDFIQSNNETTPEKAFIKEVMGTGD"
     gene            complement(5877..6602)
                     /gene="mcbE"
                     /locus_tag="JQG95_003163"
     CDS             complement(5877..6602)
                     /gene="mcbE"
                     /locus_tag="JQG95_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17 immunity protein McbE"
                     /protein_id="HAY5889633.1"
                     /translation="MVTLKMAIIFLMEQIRTPFSLIWTIMSPTVLFFFLHFNEIELHY
                     GDTAWLGKQISWFVGYISFSVVLFNYCLYLVGRRESGFIATFVHNMDGRLLFIRSQLI
                     ASLIMSILYVFFFILVVLTGFQASPDYQIVMIILKSIYINAFMMVSLTFMASFRVTFQ
                     TASTIYSVLITVCMVSGIVSLKYNEGIVYWINQVNPIAIYSTILQSDQELSLMTIFFY
                     SIMLIISIISALTFKTEPVWSSQ"
     gene            complement(6612..7802)
                     /locus_tag="JQG95_003164"
     CDS             complement(6612..7802)
                     /locus_tag="JQG95_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HAY5889634.1"
                     /translation="MINVYSNLMSAWPATMAMSPKLNRNMPTFSQIWDYERITPASAA
                     GETLKSIQGAIGEYFERRHFFNEIVTGGQKTLYEMMPPSAAKAFTEAFFQISSLTRDE
                     IITHKFKTVRTFNLFSLEQQEIPAVIIALDNITAADDLKFYPDRDTCGCSFHGSLNDA
                     IEGSLCEFMERQSLLLYWLQGKANTEISSEIVTGINHIDEILLALRSEGDIRIFDITL
                     PGAPGHAVLTLYGTKNKISRIKYSTGLSYANSLKKALCKSVVELWQSYICLHNFLIGG
                     YTDDDIIDSYQRHFMSCNKYESFTDLCENTVLLSDDVKLTLEENITSDTNLLNYLQQI
                     SDNIFVYYARERVSNSLVWYTKIVSPDFFLHMNNSGAININNKIYHTGDGIKVRESKM
                     VPFP"
     gene            complement(7783..8601)
                     /locus_tag="JQG95_003165"
     CDS             complement(7783..8601)
                     /locus_tag="JQG95_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HAY5889635.1"
                     /translation="MSKHELSLVEVTHYTDPEVLAIVKDFHVRGNFASLPEFAERTFV
                     SAVPLAHLEKFENKEVLFRPGFSSVINISSSHNFSRERLPSGINFCDKNKLSIRTIEK
                     LLVNAFSSPDPGSVRRPYPSGGALYPIEVFLCRLSENTENWQAGTNVYHYLPLSQALE
                     PVATCNTQSLYRSLSGGDSERLGKPHFALVYCIIFEKALFKYRYRGYRMALMETGSMY
                     QNAVLVADQIGLKNRVWAGYTDSYVAKTMNLDQRTVAPLIVQFFGDVNDDKCLQ"
     gene            complement(8603..9490)
                     /locus_tag="JQG95_003166"
     CDS             complement(8603..9490)
                     /locus_tag="JQG95_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McbB family protein"
                     /protein_id="HAY5889636.1"
                     /translation="MVLPDIKKGKDMINILPFEIISRNTKTLLITYISSVDITHEGMK
                     KVLESLRSKQGIISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIIS
                     DVPHLFSNAREQWKSDGVFVSHIIDIKDNNINVSDSTLIWLHLENYHSDIVKRIYSKF
                     ESNPGVAFIQSYYLKESFRIDGVYSPDLGTPCHFCHIDRWLSREEKSFRRNEMSWANL
                     LQLLKKYQMTLPALALGESERGFSYHLIKRRLQELTGTSLVKSHVDNFMSSVSADLIT
                     CILCKEPVIHWQACSCLER"
     gene            complement(9517..9720)
                     /locus_tag="JQG95_003167"
     CDS             complement(9517..9720)
                     /locus_tag="JQG95_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17"
                     /protein_id="HAY5889637.1"
                     /translation="MELKASEFGVVLSVDALKLSRQSPLGVGIGGGGGGGGSCGGQGG
                     GCGGCSNGCSGGNGGSGGSGSHI"
     gene            10250..10702
                     /locus_tag="JQG95_003168"
     CDS             10250..10702
                     /locus_tag="JQG95_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY5889638.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPIENKGIYAGNPARLIRIL"
     gene            complement(11105..11455)
                     /locus_tag="JQG95_003169"
     CDS             complement(11105..11455)
                     /locus_tag="JQG95_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
                     /protein_id="HAY5889639.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADLNSSTIIQSSSQSEQK
                     SDEETKKNAMKLIN"
     gene            complement(11458..12420)
                     /gene="parM/stbA"
                     /locus_tag="JQG95_003170"
     CDS             complement(11458..12420)
                     /gene="parM/stbA"
                     /locus_tag="JQG95_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HAY5889640.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
BASE COUNT         3495 a         2800 c         2705 g         3683 t
ORIGIN      
        1 actgatcagc aatcaccgga acatcgtagc tgacgccttc gcgttgctca gttgtccaac
       61 cccggaaacg ggaaaaagca agttttcccc gctcccggcg tttccataac tgaaaaccat
      121 actatttcac agtttaaatc acattaaacg acagtaatcc ccgttgattt gtgcgccaac
      181 acagatcttc gtcacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga
      241 aacgatccct gtttgagtat tgaggaggcg agatgtcgca gacagaaaat gcagtgactt
      301 cctcatctgg cgcaaaacga gcatacagaa agggtaaacc tttgagtgaa gcagaacgac
      361 agagagcagc ttctgcgcgc aaacgatctg tatgtaaaga gatcaaggtt ttcgtcagac
      421 cagaactgaa aaactacctt accagtctgt gtgcagagga aggggtaact caggcagagt
      481 tgatagaaag gcttatcgag aaagaggcct gtcacaggaa catgatgtga tgggatcaca
      541 ttcttgctag ttttttctgt cagtgctaga ttactgatcg tttaaggaat tttgtggctg
      601 gccacgccgt aaggtggcag ggaactggtt ctgatgtgga tttacaggag ccagaaaagt
      661 gaaaaccccg ataatcttca gcaagtttgg cgactgagaa gaagattaac ggggctaaca
      721 agaaaccgca tagaagctgt tgctctatgc ggggagtata gttatatgcc cggaaaagtt
      781 caagacttct ttctgtgctc actccttctg tgcaacataa gtgcaggatg gtgtgactga
      841 tcttcaccaa acgtattacc gccaggtaaa gaacccgaat ccggtgttta caccccgtaa
      901 aggtgccgaa acgctgaagt tctgcgaaaa actgatggaa aaggcggtgg gcttcacctc
      961 ccgttttgac ttcgccattc atgtggcgca tgcccgttcg cgtggtctgc gtcgacgcat
     1021 gccaccggtg ctgcgtcgac gggcaattga tgcgctgctg caggggctgt gtttccacta
     1081 tgacccgctg gccaaccgcg tccagtgctc catcaccaca ctggccattg agtgcggact
     1141 ggcgacagag tccggtgcag gaaaactctc catcacccgt gccacccggg ccctgacgtt
     1201 cctgtcagag ctgggactga ttacctacca gacggaatat gacccgctta tcgggtgcta
     1261 cattccgacc gacatcacgt tcacaccggc actgtttgcc gctctcgatg tgtctgaggt
     1321 ggcggtggct tccgcacgcc gcagtcgtgt ggaatgggaa aacagacagc gcaagaagca
     1381 gggactggat gcactgggta tggatgagct gatagcgaaa gcctggcgtt ttgtgcgtga
     1441 gcgtttccgc agttaccaga cagagcttaa gtcccgtgga ataaaacgtg cccgtgcgcg
     1501 tcgtgatgcg aacagggaac gtcaggatat cgtcaccctg gtgaaacggc agctgacgcg
     1561 tgaaatctcg gaagggcgct tcactgctaa tggtgaggcg gtaaaacgcg aagtggagcg
     1621 tcgtgtgaag gagcgcatga ttctgtcacg taaccgtaat tacagccggc tggccacagc
     1681 ttccccctga aagtgacctc ctcagaataa tccggcacgc gccggaggct tccgcacgtc
     1741 tgaagcccgc cagcgcacaa aaaatcagca ccacatacaa aaaacaatct catcatccag
     1801 cttctggtgc atccggtctc ccctgttttc gatacaaaac acgcctcact gacgggtaat
     1861 tttgcttatc cacatataac tgcaagggac ttccccataa ggttacaacc gttcatgtca
     1921 taaagcgcca gccgccagcg ttacagggtg caatgtatct tttaaacacc tgtttatatc
     1981 tcctttaaac tacttaatta cattcattta aaaagaaaac ctattcactg cctgtcctgt
     2041 ggacagacag atatgcacct cccaccgcaa gcggcgggcc ccgaccggag ccgctttagt
     2101 tacaacactc agacacaacc accagaaaaa ccccggtcca gcgcagaact gaaaccacaa
     2161 agcccctccc tcataactga gaagcgccgc cgtcccgccc aaagggcggg aacaacatcg
     2221 cttttaatta tgaatgttgt aactatactc cgtcatcgct gtcagtcttc tcgctggaag
     2281 tattgagtac acgctcgtaa gcggccctga cggcccgcta acgcggagat acgccccgac
     2341 ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca gaagctctct catggctgaa
     2401 aacgggtatg gtctggcagg gcaagggatg ggtaaggtga aatctatcaa tcagtaccgg
     2461 ctgacgccgg gcttcggcgg ttttactccg gtatcacatg taaccacagc gtgccgcctt
     2521 ccatgccgct ggcgcggcat aagcatatgt tggtggctgt gtctgaaagg ttatatactc
     2581 tgcataaaac ctgacaacac gcagattgcc aggcaaatga gcattatgca tccggttatc
     2641 gcctgcctgt gaaacaatat caccggggcg aatatcgata ctctgactcg ttatacccat
     2701 gcgtgtggca tcaaaaaacc tgcaattgcc ctttattgaa tgaaaggggg agtcaattat
     2761 gaatagcacc acacattacg aaaacgccaa tttcctgcgc gagctggccg aaagcctgcc
     2821 tcgtattttt ccagaaggca gtacagacaa gtctgcacta ttgcaacgcc tcgctaatga
     2881 agaactggca cgggcggaat acgacgaaca gatccgagcg aaagttgccg ctgcccgcgc
     2941 cgataagcgg ccgggaatga gttccgcgca actgcgccag caactacagg gacgttatca
     3001 ggaactgcgc aatgagttat gaaatagaat ggcgaccagg tgcgattgag gatataacgg
     3061 cgttgtttga atatttggtc gagaatgcat ctctttggga tgcaaggaac gtgacagagc
     3121 gtctgatcac tgcaaccgat cgcctggctg attaccccag gctgtatgaa acggatgaac
     3181 gctatggtga aggggttcgc cgtattagct tacttggaca gcatgtactt tatgaagtga
     3241 atgatgaggc cagtcgtgtc aatatcctgg cggttgtagg ccagcgtcag aatccccgat
     3301 tcattctttg agtgtccggc ttggaactcc atcttcagag aatcgatcaa cgttctttag
     3361 ctccttccac taacagccga cctatctcaa acaccgcagc tgaccgtccc gacttcaccg
     3421 cttcggcttt ctgtccctca acaaaccagt tattaccgtc cgttcggacc aactcaaaca
     3481 tccatgacat atcgtaaacg tgttccggga tcagcgaccc ttctccggcc agaatacccg
     3541 ccgttatgga gtggttgccc ccgtgcacaa agccaatacg ccagggcaac cacaggtcaa
     3601 catagtggtt gtttttatct tgcttccacg gcttcccctt atgggttcca atgcgtgaaa
     3661 cgttatcgat atagcgtggt aggctccaag gccacggcag cactatgtcg cttgccagcg
     3721 acaacggata atcagcgccg ttcatctcac actctttcat cttttcgtaa gattgtattt
     3781 gggtaagtcc cttaaagaag aactcctgcg gggtcatatc cgggcgggca tctgttccct
     3841 cctcagctac cgccagcaag aaatcactct gtactggccg cacgatagcg cgaatgagat
     3901 ctggtagcgc cttaacgggt agtttctctg ccacagaaag cagatgattg aagcgttttt
     3961 tttcattggc tatagttgct ttatctttct tgtttcggtt ccagaacatc actgtctgta
     4021 gccctcctta tcgtgatcag gatgatggtt ttcattctaa tggtgtagta gtttcacgtc
     4081 acgcgcacgt gcatattctt cgaaagcttc ggcgttagcc ggtcaaccag ggggttgagg
     4141 cccaaccgta cgagatcgta cgctaagttc attttttgag cagaagaggt gagtaaagaa
     4201 aacgagggtt ttgtttactc ttcccgtcaa tgccagtagc catgcggttt cagggacttt
     4261 cagtgttaat aaactcggta atcaaatcaa cgtaaacctg cctaacaacg ccacgacttt
     4321 attgacttaa aatcatatag ttagcttctt agcgtacgtt tccgctccat tgggcttcaa
     4381 accccacacc caatgacatg agaaaagctg gatctgattc tcaaaatgct gggataccca
     4441 tcgagatgga gaactcagtc aatgttccat tctttctgaa atatgtgtgg ggacactgat
     4501 ctgtcgggag ccggatgcgg tagttccgca ggtccggttc tgaggagggg cccgtccggg
     4561 caaccggcgg gtctactcac ctcatccccc tacaaccact cccattcgtt taagtatttt
     4621 taaacctgct tcctgtggta tctttataga taagatcctg tttatctcag gatttatatc
     4681 gagaccttct gtcattgaaa aatcacagtg agacaaatca gaatgtctga actctgtatt
     4741 attaaactcc gaaccagtaa aatcagattt acgaaggtct gtttcctcaa ataaacaatc
     4801 acggaagcga ctggataaaa aaatagattt ctgtaaccgt aaactaataa aatcaacaaa
     4861 tctcaaatca caatttacaa gagaaaaagt acagggaaac ataatgtctg ctgcatttac
     4921 tccttgtaat aaacaatctt tgaattcaca gtcaataagt ttaaaaaaat taagttttaa
     4981 attctttatt gagcagttta cgaagcgaca tttttcgaac tcacaatcgc gaaagttaaa
     5041 gttgtcaagc tgtattcgtt caaatataca attgtaataa tttacacctg ataattcact
     5101 ttcactgagg tgtcgttttg tgattctttt ttctattata tccatgtgtc agtctcctgt
     5161 tcccataact tctttaataa atgccttttc tggagttgtt tcattattgg attgaataaa
     5221 atcactcata tcagaataac gatttatact attttttgaa agcatataga aggggatttt
     5281 atctcttgtt aattcatcaa aaatatgagt tgagaaaaat acagttttcc cgtcagcggt
     5341 gatcttattt atcagttccc ataacatcat tctgtactga atatcaatgc ctacagttgg
     5401 ttcatccagt aaaaataaac gggcattttt gcaaagcgtt agcatcaaag atatgataag
     5461 ccaacgcttc tctccataac ttaccgtgta agtcctttta cgtcttattt tatcatagcg
     5521 aatgaaaaaa ttatcattcc atttatgatt aagctctgtc catgcctgtt ttttagttaa
     5581 cttattgaag cagcaaatca aatcaagaac ctcctcattc cttagtgtac ctgaaagatt
     5641 tatgacttga ggtagactta ctaattctcc accagagaaa tttcgcttga gggtcccttc
     5701 ataattactg actccgcccc tgataaggtt aaaaagagtg gtcttcccgg ctccattttc
     5761 tccaagcaga cctatcaact ctccctgctc aatcttaagc gacagattat tgaaaacagg
     5821 gggtaaattt actttatatg aaaagctcag agagttgatt tctaaaagag gtatggtcat
     5881 tgagaactcc agactggctc agttttgaag gttagtgctg aaataatact tatgattaac
     5941 attatactat agaaaaatat cgtcatcaga gacaactcct gatctgactg gagtattgta
     6001 gaatagatgg cgataggatt aacctgattt atccagtaaa cgatcccctc attatatttt
     6061 aatgatacaa ttcctgatac catacagaca gttattaata cagaatagat agtgctggct
     6121 gtttggaatg tgactctgaa cgatgccatg aaagtgagtg aaaccatcat aaatgcattt
     6181 atgtatatcg attttaaaat aatcattact atttgataat ctggacttgc ctggaatcct
     6241 gttagtacga ctaaaatgaa aaaaaacaca tacaaaatag acatgataag tgaggctatt
     6301 aattgagaac gaataaataa tagtcgacca tccatattat gcacgaaggt agcaataaaa
     6361 ccactttctc ttcttccgac cagatacaga cagtaattaa acagaacaac agaaaaagaa
     6421 atgtagccta caaaccatga tatttgtttt ccgagccatg ctgtatcacc ataatgaagt
     6481 tcaatttcat tgaaatgaag gaaaaagaac aacactgtag gtgacataat tgtccatatt
     6541 aagctgaaag gggttctgat ttgttccatc agaaaaatta tcgccatttt taatgtaacc
     6601 atttgttatc cttatgggaa tggtaccatc tttgattctc tgactttaat accgtccccg
     6661 gtatggtaaa ttttattatt aatgtttatt gcacctgagt tattcatatg aaggaaaaaa
     6721 tcagggctta ctatttttgt gtaccagaca aggctgttac ttactctttc cctggcatag
     6781 taaacaaaaa tattatcaga aatttgttga agatagttta ataaatttgt gtctgacgta
     6841 atattttcct caagcgttaa cttgacatca tcagacagta gtaccgtatt ttcacacaaa
     6901 tccgtaaacg actcgtactt gttgcatgac ataaagtgac gctggtaact atcaataatg
     6961 tcatcatcag tataaccgcc aataagaaag ttgtgcaggc atatatacga ttgccacaat
     7021 tccactacgg atttacaaag tgcttttttc agactattag cataggataa tccggtactg
     7081 tattttattc gactgatttt gttttttgtg ccatacaggg ttagtactgc gtgtccagga
     7141 gctccgggca gggtgatatc gaaaatcctg atatctcctt ctgacctgag agccagtaaa
     7201 atctcatcta tatgatttat gcctgttact atttcactgg atatttcagt attggctttt
     7261 ccctgtaacc agtaaagaag gagggactgt ctctccataa attcacacaa ggaaccttct
     7321 atggcatcgt tcaaactacc atgaaagcta catccgcatg tatctctgtc aggataaaat
     7381 ttcagatcat ctgcagcggt tatattgtcg agtgcaatta tgactgcagg tatttcttgt
     7441 tgttcaaggc taaacagatt aaaggttctg accgttttaa atttatgggt tatgatttca
     7501 tcgcgggtca gtgatgagat ctgaaaaaat gcttcggtaa aagcctttgc agcagatgga
     7561 ggcatcatct catataatgt tttctgacca ccggtgacta tctcattaaa aaaatgacgg
     7621 cgttcaaaat attcacctat tgccccctga attgacttca gagtttcacc ggccgcgctg
     7681 gctggtgtaa tacgctcata gtcccatatc tgagaaaacg ttggcatatt tctgttcagt
     7741 tttggactca tggccattgt ggccggccat gcggacataa ggttactgta gacatttatc
     7801 atcgtttaca tctccaaaaa actgaacgat cagtggcgct acagtcctct gatccagatt
     7861 cattgttttt gctacgtatg aatcggtata tcccgcccat acccggtttt tcagtcctat
     7921 ttgatctgca accaatactg cgttctgata catcgaacct gtttccatta aggccatccg
     7981 gtatcctctg tagcgatatt tgaacaaagc tttttcaaaa ataatgcaat agacgagagc
     8041 aaaatggggt ttaccaagac gttccgaatc cccaccggac aggcttcggt agagtgactg
     8101 agtattacat gtagcaacag gttccagtgc ctgacttagc ggcaggtagt gataaacatt
     8161 agttcctgcc tgccagtttt ctgtattttc agataatctg cataaaaaaa cttcaatcgg
     8221 gtacaatgcc cccccagaag gataaggtct tcttacagag ccaggatcag gtgagctgaa
     8281 tgcattgact aataactttt caatagtacg aatggaaagt ttatttttgt cgcaaaagtt
     8341 tattcctgat gggagccttt cacgactaaa attatgtgat gaggatatgt ttattacgga
     8401 gctgaaacct ggcctgaaga gaacttcttt attttcaaat ttctccagat gggcaagagg
     8461 taccgcggac acgaaagttc gttcagcaaa ttcggggagg gaagcaaagt tacctctgac
     8521 atgaaaatct ttaacaatgg ccagaacttc aggatctgtg taatgcgtta cttccactaa
     8581 agagagttcg tgttttgaca tgttatctct ccagacagct gcaagcctgc cagtgaatta
     8641 ccggctcttt acataaaata caggtgatta aatcagcgct aacagatgac ataaagttat
     8701 cgacatgact tttaaccagt gaagtacctg tcaactcctg aagtcgccgt tttattaaat
     8761 gatagctgaa tcctctttct gattcgccta aagccaatgc tggtagtgtc atttggtatt
     8821 ttttcagtaa ctgaagtaag tttgcccagg acatttcgtt acgtctgaag cttttttctt
     8881 ccctgctcag ccatctatct atatgacaaa aatggcaggg agtaccaaga tcaggtgaat
     8941 atactccatc tatcctgaat gactccttta ggtagtagct ttggatgaag gcaacaccag
     9001 ggttactttc aaattttgaa tagattcttt ttacaatgtc tgaatgatag ttttcgagat
     9061 gtaaccagat tagtgttgaa tcgctcacat taatattatt atcttttata tcaatgatat
     9121 gagaaacaaa aacaccatca gatttccatt gttcccgggc attactgaaa agatgtggaa
     9181 catcgcttat tatgactgag tttacccaga gaggtgatgt tttcgtttta tttatcacgc
     9241 ctgtggtaat aagaaactct ttgcccttgt ctttatcaat aagcgattcg tccagtagtt
     9301 tatcaaggag gtattcagaa ataataccct gtttggatcg taggctttcg agtacttttt
     9361 tcattccctc atgtgtaatg tctactgaag aaatgtaggt aataagcagg gtctttgtat
     9421 ttctggaaat tatctcaaac ggcagaatat tgatcatatc ttttcctttt ttaatatcag
     9481 ggagcaccat gctccctgaa cggttaattc aacgtatcag atatgtgaac cacttccgcc
     9541 gctgccaccg tttccaccac tacaaccgtt gctgcaacca ccacaaccgc caccttgacc
     9601 accgcagcta ccgccgccgc cgccaccacc accaatgcca acacctaatg gagactggcg
     9661 tgataattta agagcatcaa cggacaaaac tacaccaaat tcactcgctt ttaattccat
     9721 aaggactcct tttttaaggg aaattaatgc ttatgacatc tgaatagtat gttggccaac
     9781 actagttact tgccgtaatt aactatattt tgcaatgata atttgaacta aatttgtacg
     9841 cctgttaaat atttaataat aatatcagtt ggttatgttt atgctgttat tgttttttta
     9901 caaaaattaa tattgtgttt acattttgct ttcagttgct gtcaggatgt cacactaccc
     9961 ctttaagccc ccactggtct ggcagggccg ggggtgggta aggtgaaacc tgtcaatcag
    10021 taccggctta cgccggtatc atatgcaaca actgagtgcc gccttccata tcatatcatt
    10081 tgcccgtttc ccgccgtgca gatccggttt ccggctgaag tggatccaga cctgatttgt
    10141 tgctgagtat cggtaaaaga gcggcttata atctattctg aaacatggca agtgaatctg
    10201 ggctgtataa ttccggcaag tgtcatctgt atttaaaggt cgtgtgagta tgaaatttat
    10261 ggaatgtgct gtccgggatg tgatttatgg gaccaatgtc cgtattgtca aaccggtcaa
    10321 catttatgaa tgtgaactca gggacaatgt gtttgtcggc cccttcgttg agatacagaa
    10381 aggctgtgtc attggtagtg gaagccggat acagtcgcac acgtttattt gtgagaacgt
    10441 aacactgggg gagaactgct tcattggtca taacgtgacg ttcgccaacg atctgtttcg
    10501 ttcaggagca ccagatccgt caccggataa ctggatcagc attaccctgg gggattcggt
    10561 tactgttggc agtggagcca ttattctgtc tccgtatata tgcagcggag ctgttattgg
    10621 tgcgggggca gttgtagtaa aaccgattga gaacaaaggc atttatgcag gaaacccggc
    10681 aagactgatc agaatattat gaaaaagaaa agggccgtta ctggcccttt ttatcagagg
    10741 taggtctgtg aatacggcag gtgtacagaa atcgactgcc ggggatcgat ttccagggcc
    10801 gcccggtatt ctgcgtcagt gatatcacct tttttcagta aataaccgag agcatcgttt
    10861 cccagccctg acagtatgcg tattctggcg atccggactg gcaaataacc catcaaaaac
    10921 gttattgcca gacattgcga actcaccaat tttgattatt ggagccactt tttcataagt
    10981 tccgtgaaat tatttcataa taacgaccct gtttatttta tgtaatgact ggcaataaaa
    11041 taacttgatt aagttccctt tatccagcct gatagtggat aaaggaaact caatcataat
    11101 ttaattagtt tattaacttc atcgcattct tttttgtctc ctcatcactt ttttgctccg
    11161 attgtgaaga ggactgaatg attgttgaag aatttaaatc ggccatctct tttggaaata
    11221 gcgatctcaa tatattcacg atatctgaaa atgtggtttc tttcgtcagc agttcacata
    11281 aaaggaaagg ggccctggga tcttgtctgt ataaggccag acctgccgtc agtgcagccc
    11341 ggttaaggcg gctgcgttcc ccttgcggga tactgtccag tgtatcacaa acaattttat
    11401 ctgtttgatt tacatctgga tttagcttaa aggcaatggt tctgcgcttg tccatcatta
    11461 attacctatg agatacatac cgttaactaa atcatattga gagttattgg ttttaaaaaa
    11521 acgttcatca cgaatctgtg tgtgtttttt tactgcatcg catattaatt ctgcgccacc
    11581 acctataacc ataacatgag tataaccaga aaattcattg agtgtattta atacacgttg
    11641 ctcaagttta cgaagtgctt cattcattgc ttcggtgact attgatattt tgttctcatc
    11701 attaattcgt tgcttcagat agttattatc ttttctgtga ataattatat catcagcaag
    11761 atagctactt ccttttgttc tcgcaagaga aagggcatct tttactgcag atgtgaccag
    11821 agatacacca agagatgagt ctccgtatat tttactgatc cccgataatt tccccattac
    11881 ctgagaaata tctaatgtgg tgcccccgag atctataatt aataaagaat ctaactcatc
    11941 cagttcttgt agaacttcat aacctgccgg tatagattca ggcatgactt ttacatcttt
    12001 tattgtgaat gtatccccac catttaatgt aatttttttc cggaagtttg ctttcttacg
    12061 ctcaatattt tccgtattgg gttggttatt cctgtcgtaa tactctgtca gaggaagtgt
    12121 gcaaacaata tccacttcgc ttaccggcag accactggtc agtaaggcgt ggtgcaccgc
    12181 aacgacatta acgtcgctgt attgccatgc gatattggtt gtgactacag catccgggct
    12241 gattggatca aatgaatact gttcgccgtt cagtgtgtag ttaaagacct ttttatcacc
    12301 aaaagaaact gcccactcgc gtttgaagct gttcgggcta atgtgctgtt taattgttcc
    12361 gtcgctttcc tgccactgta gtttgatgtt tgttgaaccg tcatcaatga ataccaacat
    12421 ttataaaact ccttatggtg ttttttttgg tgtttgtttg ggtgtgtttt gggctttaaa
    12481 tgggtttatt tgtcaagttt accccatttt aactatcaat caatgattat ttgtcttgtt
    12541 ttggtgtttt gttgggtttg gtatgggttg tttttgggtt ttgtttggcg gataacaaaa
    12601 gatgtcgccg ggctcctctt tttactgcgc cggctgacgc ggcgcggcag gaacgctgcc
    12661 tgtggtcagt gtcccgcgtc cgg
//
LOCUS       DADLCQ010000116        12413 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000116 DADLCQ010000000
VERSION     DADLCQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.116"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1198
                     /gene="edd"
                     /locus_tag="JQG95_003171"
     CDS             <1..1198
                     /gene="edd"
                     /locus_tag="JQG95_003171"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY5889641.1"
                     /translation="ALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHP
                     DSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHL
                     VAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGL
                     LHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVL
                     SGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKA
                     NGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVR
                     DGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQG
                     ATCITF"
     gene            1235..1876
                     /locus_tag="JQG95_003172"
     CDS             1235..1876
                     /locus_tag="JQG95_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY5889642.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1932..3110)
                     /gene="purT"
                     /locus_tag="JQG95_003173"
     CDS             complement(1932..3110)
                     /gene="purT"
                     /locus_tag="JQG95_003173"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY5889643.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            3244..3534
                     /gene="yebG"
                     /locus_tag="JQG95_003174"
                     /pseudo
     CDS             3244..3534
                     /gene="yebG"
                     /locus_tag="JQG95_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3601..3957
                     /gene="yebF"
                     /locus_tag="JQG95_003175"
     CDS             3601..3957
                     /gene="yebF"
                     /locus_tag="JQG95_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY5889644.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            4284..4943
                     /locus_tag="JQG95_003176"
     CDS             4284..4943
                     /locus_tag="JQG95_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY5889645.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5153..7213
                     /gene="ptrB"
                     /locus_tag="JQG95_003177"
                     /pseudo
     CDS             5153..7213
                     /gene="ptrB"
                     /locus_tag="JQG95_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="JQG95_003178"
     CDS             complement(7210..7872)
                     /gene="exoX"
                     /locus_tag="JQG95_003178"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY5889646.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLNSMSPELRLTLKHYLE
                     NT"
     gene            complement(7896..8552)
                     /locus_tag="JQG95_003179"
                     /pseudo
     CDS             complement(7896..8552)
                     /locus_tag="JQG95_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(8654..8884)
                     /locus_tag="JQG95_003180"
     CDS             complement(8654..8884)
                     /locus_tag="JQG95_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY5889647.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9023..9397
                     /gene="yobA"
                     /locus_tag="JQG95_003181"
     CDS             9023..9397
                     /gene="yobA"
                     /locus_tag="JQG95_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY5889648.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            9401..10273
                     /gene="copD"
                     /locus_tag="JQG95_003182"
                     /pseudo
     CDS             9401..10273
                     /gene="copD"
                     /locus_tag="JQG95_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            10286..10627
                     /locus_tag="JQG95_003183"
     CDS             10286..10627
                     /locus_tag="JQG95_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY5889649.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(11020..12096)
                     /locus_tag="JQG95_003184"
                     /pseudo
     CDS             complement(11020..12096)
                     /locus_tag="JQG95_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12062..12343)
                     /locus_tag="JQG95_003185"
     CDS             complement(12062..12343)
                     /locus_tag="JQG95_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY5889650.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         3079 a         3032 c         3137 g         3165 t
ORIGIN      
        1 ggccttactg gagtcagaag ccgcgtctta ccatgcgccg ggaacatgta ctttctacgg
       61 tactgccaac accaaccaga tggtggtgga atttatgggg atgcagttgc caggctcttc
      121 ttttgttcat ccggattctc cgctgcgcga tgctttgacc gctgccgctg cgcgtcaggt
      181 tacacgcatg accggtaatg gtaatgaatg gatgccgatc ggtaagatga tcgatgagaa
      241 agtggtggtg aacggtatcg ttgcactgct ggcgaccggt ggttccacta accacaccat
      301 gcacctggtg gcgatggcgc gcgcggccgg tattcagatt aactgggatg acttctctga
      361 cctttctgat gttgtaccgc tgatggcacg tctctacccg aacggtccgg ccgatattaa
      421 ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt gaactgctca aagcaggcct
      481 gctgcatgaa gatgtcaata cggtggcagg ctttggtctg tctcgttata cccttgaacc
      541 atggctgaat aatggtgaac tggactggcg ggaaggggcg gaaaaatcac tcgacagcaa
      601 tgtgatcgct tccttcgaac aacctttctc tcatcatggt gggacaaaag tgttaagcgg
      661 taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg gttgagaacc aggtgattga
      721 agcgccagcg gttgtttttg aaagccagca tgacgttatg ccggcctttg aagcgggttt
      781 gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg ccaaaagcga acggaatgcc
      841 agaattacat aaactcatgc cgccacttgg tgtattattg gaccggtgtt tcaaaattgc
      901 gttagttacc gatggacgac tctccggcgc ttcaggtaaa gtgccgtcag ctatccacgt
      961 aacaccagaa gcctacgatg gcgggctgct ggcaaaagtg cgcgacgggg acatcattcg
     1021 tgtgaatgga cagacaggcg aactgacact gctggtagac gaagcggaac tggctgctcg
     1081 cgaaccgcac attcctgacc tgagcgcgtc acgcgtggga acaggacgtg aattattcag
     1141 cgccttgcgt gaaaaactgt ccggtgccga acagggcgca acctgtatca ctttttaaga
     1201 cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa aactggaaaa caagtgcaga
     1261 atcaatcctg accaccggcc cggttgtacc ggttatcgtg gtaaaaaaac tggaacacgc
     1321 ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc gttctggaag tgactctgcg
     1381 taccgagtgt gcagttgacg ctatccgtgc tatcgccaaa gaagtgcctg aagcgattgt
     1441 gggtgccggt acggtgctga atccacagca gctggcagaa gtcactgaag cgggtgcaca
     1501 gttcgcaatt agcccgggtc tgaccgagcc gctgctgaaa gctgctaccg aagggactat
     1561 tcctctgatt ccggggatca gcactgtttc cgaactgatg ctgggtatgg actacggttt
     1621 gaaagagttc aaattcttcc cggctgaagc taacggcggc gtgaaagccc tgcaggcgat
     1681 cgcgggtccg ttctcccagg tccgtttctg cccgacgggt ggtatttctc cggctaacta
     1741 ccgtgactac ctggcactga aaagcgtgct gtgcatcggt ggttcctggc tggttccggc
     1801 agatgcgctg gaagcgggcg attacgaccg cattactaag ctggcgcgtg aagctgtaga
     1861 aggcgctaag ctgtaaaagt caaaatgccc gatctaggat cgggcatttt tgtagcgttt
     1921 ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc gcgttcaatg
     1981 gcgtcaacaa cactctctga agtagccagt accaccccca gacgacggct gccatcaatt
     2041 tccggcttac caaataaacg aatctgcaaa tctgcgccta cggcattctg cacattatca
     2101 aacgtgacat tctgactggt cagttgtggc agaataacgg cagaagctgc aggaccatac
     2161 tgacggatcc cgccaacagg aagtccgagg aaggcacgta catgcagggc aaactctgag
     2221 agatcttgag aaattaacgt caccatcccg gtatcatgtg gacgagggga gacctcactg
     2281 aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagaccata accgcccagt
     2341 gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg gctcatttgc
     2401 tgtggttgcc aggattcacg gtagtcgcca tcttcctggc gatgacctac tggtgcacag
     2461 aaatggacgc catccaccgc gctgacggtt agcagggtaa tttcgaagtc aaacttaacg
     2521 acgccttcaa caattacgcg gcccgctccg gcgcgaccgc cttgctgagc gtattcccat
     2581 gcctgagcaa gttgctctgc agagcgaata aacgtctgcc ccttgccgga agagctcatc
     2641 accggtttta caatgcaggg atagccaatg gcggcaaccg cctcgcggaa aaggcttttg
     2701 ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc caggcgacgg
     2761 ataccctcgc gattcatcgt taatttcgtt gcgcgagcgc aggggacaac attcagtcct
     2821 tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg cacgatataa
     2881 tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat attaatgaca
     2941 tgggagcgat gcgcgacatg catggctggt gcgtcggcat agcgatcgac ggcaatcacc
     3001 tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc ggagcctaat
     3061 aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat aacgaatatc
     3121 ccctgattaa ttccgctcgc agtataaacg aaaacgtttg cgtgtgtctt tattgctgat
     3181 gttgatttct caaccgaaaa gaaatatact gtataaaatc tcagttatta tgagaggctg
     3241 caaatggcgg ttgaagttta atacgtagtc attcgtgagg gcgaagagaa aatgtcgttt
     3301 accagcaaaa aggaagccga tgcttatgac aaaatgcttg atacggcgga tctgcttgac
     3361 acctagctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc cctttcgcta
     3421 tggctggcag aacaaaaaga ggtgctgagc accattctga aaaccggcaa attaccgtcc
     3481 ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc ctgaagcaac
     3541 ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat ggagaaaaac
     3601 atgaaaaaaa gaggggcgtt tttagggcgg ttgttggttt ctgcctgcgc atcagttttc
     3661 gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg tctggatgct
     3721 gccggaattg ccgcgagcgt aaaacgtgat tatcaacaaa atcgcgtggc gcgctgggca
     3781 gatgatcaaa aaattgtcgg tcaggccgat cccgtggcct gggtcagttt gcaggacatt
     3841 cagggtaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag tgccgatatt
     3901 cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg gcgttaatgg
     3961 cttctgataa ttcgattgac cgttggcgga tgcggcgcga gtgccttgtc cgtcccagaa
     4021 cgcaccacaa tcgtaggcct gagaagacgc aacagcgtcg catcgggcaa tcagcatcaa
     4081 acaatgccac tcaaaacgac aaactacttt cacctaaaat acccttatca cttgatatac
     4141 agtaaatacc ttacctggcg cgaatttctt tgaataacgg cctaccgccc cgcttatttg
     4201 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
     4261 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
     4321 ggcaaagcag ttcttctacc tcctcgtctg cggatcaggg attgggcaaa ctgttagtgt
     4381 caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cgtaaacttc
     4441 ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtagcg ggtacggtgc
     4501 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
     4561 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
     4621 ctaaaagtga tggtcatatt gatgccaatg aacgtgcggc aatcgaccag caattgcgtg
     4681 aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggcaatcgaa caaccgctgg
     4741 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
     4801 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
     4861 gtgacgcgct gaaaatcccc caggatgttc gtgaaggcat tgaacgcgat ctcgaacagc
     4921 aaaaacgcac tttggcggaa taattccgct ctctggatag cctgaagttg actatttcgg
     4981 gctatccaga accgcacagc catttgaaca ggctgatttt ctctgtctgc ataacattcg
     5041 atatccgcgc cattacctgt catcatctaa gcaatgacga ccctgtttcg cttgcatccc
     5101 cggtgagttt tgccaccctt ataagatgtt tccaaccaga aagaacaata acatgctacc
     5161 aaaagccgcc cgcattcccc acgccatgac gcttcatggc gatacgcgca tcgataatta
     5221 ctactggctg cgggacgata cgcgttctca gccaaaagtc ctggactacc tgcaacaaga
     5281 aaatagttac ggtcatcggg tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa
     5341 ggaaatcatc gaccgcattc cgcaaagaga agtttctgcg ccctacataa aaaatggcta
     5401 ccgctatcgg catatttatg aaccaggctg tgaatatgct atctaccagc gtcaatcggc
     5461 attcagcgaa gagtgggatg agtgggaaac attgctcgat gccaacaagc gcgcagctca
     5521 tagtgagttt tattcgatgg gcggaatggc gattacgccc gataacacca ttatggcgct
     5581 ggcagaagat tttctttccc gacgccagta cggcattcgt tttcgtaatc tggaaacagg
     5641 taactggtac ccggaactgc tggataacgt tgaacccagc tttgtctggg caaatgactc
     5701 ctagactttc tactatgttc gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg
     5761 tcacgccatc ggtacgccag catcgcaaga tacactgatc tacgaagaaa aagacgatac
     5821 ctattatgtc agcctgcata agacgacgtc gaagcactat gtcgtcattc atctggccag
     5881 cgccaccacc agtgaagttc gcctgctgga cgcggaaatg gccgatgccg agccgtttgt
     5941 ttttctgccg cgccgcaaag atcacgaata cagccttgat cactaccagc atcgttttta
     6001 tctgcgttcc aatcgccacg gcaaaaactt tggcttatac cgtacccgta tgcgtgatga
     6061 gcaacagtgg gaagagttaa ttccgccacg cgaaaacatc atgctggaag ggtttacgct
     6121 gtttaccgac tggctggtgg ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat
     6181 taaccgcaag acccgggaag tcattggcat tgcctttgat gatccggcct atgtgacctg
     6241 gattgcctac aatccagaac ctgaaaccgc gcgattgcgt tatggttatt cttccatgac
     6301 cacaccagac actttgtttg aactggatat ggataccggt gagcgtcgtg tattaaaaca
     6361 aacagaagtg cctggttttg atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc
     6421 ccgtgatggc gtcgaagttc cggtttcgct ggtctatcat cgcaaacatt ttcgcaaagg
     6481 acacaacccg ttgctggtgt atggctatgg ttcttacggc gcaagtattg atgccgattt
     6541 cagttttagc cgcttgagtt tgttagatcg tggctttgtc tacgccattg tccatgttcg
     6601 cggcggtggt gagctggggc aacaatggta cgaagacggt aaatttctga agaagaaaaa
     6661 tacgtttaat gattatcttg atgcctgcga tgcattgtta aaactgggct atggctctcc
     6721 ttcgctctgt tatgcgatgg gcggaagtgc ggggggcatg ttgatgggcg ttgcaattaa
     6781 tcaacgcccg gaattattcc acggcgttat cgcccaggta ccgtttgttg atgttgtaac
     6841 aacgatgctt gatgaatcaa ttcctcttac cactggtgag tttgaagagt ggggaaatcc
     6901 gcaggatccg caatattacg agtacatgaa aagctacagc ccgtatgaca acgtcaccgc
     6961 acaggcttat ccgcatttac tggtaacgac cggtttacac gattctcagg tgcaatattg
     7021 ggaaccggca aaatgggtcg ctaaattgcg cgagctgaaa accgatgacc atcttttatt
     7081 gctctgtacc gacatggact caggccatgg cggtaaatct ggccgcttta aatcgtacga
     7141 aggcgtagcg atggaatatg cttttctggt cgcgctggcg cagggaacat tacccgctac
     7201 gcctgcggat taagtatttt ccagataatg tttcagtgtt aaacgcagct ccgggctcat
     7261 gctgttcagg ttattaaata accagcgcag atagcccgga tagcgttcgg caacgtcgga
     7321 aaccgctttg ccacggtatt tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt
     7381 gatatcggcc atctgttctg ccgtccagcc ggaggtgttc ataatatcga taagcaacgc
     7441 ggcggtgata taacagtcat acaacgcgcg gtgatgatgc aggcccggcg gtgtctgtac
     7501 attgagcttg cgtgttttat ataacgccat attgctgtac ttgagccctg gccacaaacg
     7561 acgggccagt ttcatagtgc aaatccactc accgggcatc tcaggcagta cgcggcggtc
     7621 aaagctggcg ttgtgcgcga cataccattc actaccgtaa tagtgtggga tcacatcttc
     7681 aatccacggt ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg
     7741 actaatagga cgatcggggc gcaccaggag gctcatgggg ttgacgattt ttccgtcaat
     7801 gacatcaaca gaggcaatct caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat
     7861 aatgcgcaac atggcatgct ccaggccgaa aaagcctagc gtaatggaat gatatcgcct
     7921 tgccaaccct gtgatgaacg ctgaccgact aacaataatg aaccacgatc ggcgcgaacg
     7981 atgagacgac catattcatc ccatagtgca ctgttaccac gggcatttgc catcagcacg
     8041 gcaatcgagt actgatgtga aaaaaattgt aagcgacggg ccgacgccag caggtcgggt
     8101 tctcccagac attgccctgt cgtaaacagt gaacaggtcg ggtccatatc cataccttgc
     8161 ggttgttcat cgaccaccgt gatggttctg gaacgacggc ccagacatgc accatgactc
     8221 tgatggtagt tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc acgaataaag
     8281 cgatcgttat attcaacggg aaggccggca atgatggtca ttcgctaggt cgttgcggca
     8341 taacaaagcg ggtctaacag tgaaagatcg ggtggggcag gaagggcacg tcgcgaataa
     8401 tcacacccca gtaaagaaag cgatggaaaa actaacagct gacattgttg cctggcggcg
     8461 gccctcacaa actccagatg atgagctacc tgttcggtga gtgaggtttt acgaggctca
     8521 tattgcgctg ctgcaacttt ccagaacgac ataatgactt ccttttcaaa atgactgcaa
     8581 attatggggg cggtttagaa aatactcata atttagcagg cgttatgtaa gaaagtgtaa
     8641 ctctgtataa gttttattta agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag
     8701 ccaaacggtg ggcaataagc cgctcgcgaa accagctgcg caaatgttct ggctgttcac
     8761 gttcaaccgc ttcagcgatc accggcatat tgtagcgttc tttaaaagcc accccggccg
     8821 ccgccaaatc gacattcact ttatccattt ctgtttgatc cagtttagcc agattcttca
     8881 gcattcttaa tctcctcatc attcgcgggc aaaagttact aagagtgagc ctggatggca
     8941 agccggaatt gatgatcctc gactcagaca ttagacaagg ttatgctctg taaaaaagtt
     9001 attacaaaaa ggaatagata atatggcttc aactgcacgc tcccttcgct acgcgctggc
     9061 aatcctgacc acttcgttgg tgacaccttc cgtctgggca catgcgcatt taacgcatca
     9121 gtatcctgcg gcaaacgcgc aagtgacagc tgccccgcag gcaatcactt taaacttctc
     9181 ggaaggcgtt gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat
     9241 taaaacatta ccagcgaagc ggaatgagca ggaccaaaag caactgattg ttccactggc
     9301 cgattcgctg aaacccggaa cctacaccgt tgactggcat gttgtttcgg tggacgggca
     9361 taaaacgaaa ggacactaca cctttagcgt gaaataaatc atgctggcgt ttacctggat
     9421 cgcgctgcga tttatccatt tcacctcgtt gatgctggtc tttggctttg caatgtatgg
     9481 tgcctggctg gcccccttaa tgattcgtcg cttgctgacg aagcgttctc tgcgtttaca
     9541 acagcacgct gccgtctgga gtttgatcag tgccactgca atgctcgccg ttcagggcgg
     9601 gttgatggga acgggatgga cagatgtatt ttcgccaaac atttggcagg ctgttttgca
     9661 aacgcagttt ggtggcgtct ggttatggca aattgttctc gccctcgtaa cgttgatcgt
     9721 cgcccttatg caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt
     9781 tattctgctg gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacag cgaaaatcca
     9841 tcagactaat cacgcgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc
     9901 ggtgctctgg tgtatgcaac tcatcaaagg ccgttggcga cactaggcta ttcaggcgct
     9961 gatgcgtttt tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct
    10021 taatgcattg ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt
    10081 attgctcaaa gccatcctgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt
    10141 tcttgtaccg cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg tgtggatgac
    10201 taaactcgaa tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct
    10261 cgaacctttt tgatggatta cgaaaatgat gaaaaaaagt attctggcgt ttctgttact
    10321 caccagttct gcagcggcgc tggcggctcc acaggtgata accgtgagtc gttttgaagt
    10381 gggtaaagac aaatgggcgt ttaatcgcga agaggtaatg ctgacttgcc gaccgggtaa
    10441 tgctttgtat gtcatcaacc caagtaccct cgtgcagtat cctttaaacg atatcgcaca
    10501 aaaggaagtt gccagtggga agaccaatgc ccaacccatt tcggtgattc agattgatga
    10561 tcctaacaat cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact
    10621 ctgttaatta cctaaaatag ccttttgatt tccaataaaa aaaccgcctc agttctttca
    10681 ccagaacggg cggtttttaa catttaagct gatgagcacc gcgcttttta ttgaccattt
    10741 tgcacgcaaa ctggaaaacc tggcgtcgtc atctattctt aaagggcaag gcaactaagc
    10801 ctgcattaat gccaactttt agcgcacggc tctctcccaa gagccatttc cctggaccga
    10861 atacaggaat cgtattcggt ctttttttaa ttgtatttaa aatcaattag ttgcaaacgt
    10921 ctccccgaaa ttccccgaaa tttactcgaa tttctgtatt ccggtctttt ttggttatat
    10981 cacaaccaaa atacatttaa caatccattt acgttaaaat cagagcagta agtacgtttt
    11041 ttctctctca tcaagataca tttttgttgt cttctccgat gtgtggccaa gtagacgctg
    11101 agcaaattct tctccacata tttctttgta caatcgtcca gccaggcttc tgatttcgtg
    11161 aaaagttggt gggttttcac tgaacaggat acctgttaat tttctggctg cgacaaattt
    11221 ctttgtcagg ccgtccgggt gaatgctgcc gtcagggctg ttttttctaa tcccggcact
    11281 gattagataa tcccccctac ttaccatgcg gcactgttca actactgcac caagtcgtaa
    11341 accagcgaca ggaaggctga gtgacagggg gatagcaatc atcattcctg tcttaatttg
    11401 cctgatgtgg agacgatcat cataaatatc actgaaccgc atattcgtta tgtcttcgcg
    11461 acgttgtcct gttacaaggg ctaagtccat agctaatggg aaccatgccg gaagttgatc
    11521 tgctgcctcc ctgatgcagt tgtatgtctt tagtttcagc cgttctcttg taactactat
    11581 tttcggtgct cttgttggcg ttactggatt ttgagatata cgtccttcaa caatggcttc
    11641 gcggaacata tcagataaca cagaacgcat tgatcctgcc atcgtgtttt tccctccttc
    11701 aatctacaaa tcaagaaact cggcgatatg gcgagtggtt atttctgtca gtaaaacccc
    11761 tcctaatttt tcttttattg tctccagttg atttacccga gttttataag tatttctgga
    11821 cgcttttctc cttataagaa tcgttttgta acgttcaatc cagcctgcca tagtaaatga
    11881 gtcgacccct ttaagctttt caattagggc agcaggagag tagtttttgt atatataatg
    11941 atttgcttca attgcctgcg ctactgcatc tcttcttgaa attttaccta gtgtaaattc
    12001 ttctttcgtc agagggttgc gccagtaata tgctttgtct ctccttcgat atgttaagtt
    12061 tctaggcaaa ttgggatcgt attttttccg ctgcatgttt taacttctcc attaacggac
    12121 tgtctctccc ttgtcgccca ttaggctgat ggtgtgttat atcggtatca accttatttg
    12181 ggttgatata gaaagcctcc ggaaccaccc tgtaactcct cccgtgtagt tcaggtgcag
    12241 gataaatgtt tccattcctt gcccatcgtc tcagcgttga tattgatggt gggttatccg
    12301 gatatctgag ttttccccac gttttgagtg tcacaagatt cattgccata cctctcacga
    12361 tatgaccgcc agtaaatata cagaatactg gcgggtgtgg ttgattttta ata
//
LOCUS       DADLCQ010000117        12061 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000117 DADLCQ010000000
VERSION     DADLCQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.117"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JQG95_003186"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JQG95_003186"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQG95_003187"
                     /pseudo
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JQG95_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQG95_003188"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JQG95_003188"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY5889651.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQG95_003189"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JQG95_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /locus_tag="JQG95_003190"
     CDS             complement(3361..4695)
                     /locus_tag="JQG95_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5889652.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGLKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="JQG95_003191"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="JQG95_003191"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY5889653.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVEIASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JQG95_003192"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="JQG95_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY5889654.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="JQG95_003193"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="JQG95_003193"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAY5889655.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="JQG95_003194"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="JQG95_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY5889656.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="JQG95_003195"
     CDS             9553..10332
                     /locus_tag="JQG95_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY5889657.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="JQG95_003196"
     CDS             10322..11131
                     /locus_tag="JQG95_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY5889658.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="JQG95_003197"
     CDS             11218..11652
                     /locus_tag="JQG95_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY5889659.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12061
                     /locus_tag="JQG95_003198"
     CDS             11649..>12061
                     /locus_tag="JQG95_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY5889660.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3250 c         3073 g         2859 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag caccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 acaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga caccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgagaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaatc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcagttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctggca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaacccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggtgggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggttt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 c
//
LOCUS       DADLCQ010000118        12023 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000118 DADLCQ010000000
VERSION     DADLCQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.118"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            347..640
                     /locus_tag="JQG95_003199"
     CDS             347..640
                     /locus_tag="JQG95_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889661.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JQG95_003200"
     CDS             712..1371
                     /locus_tag="JQG95_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY5889662.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JQG95_003201"
     CDS             1448..1909
                     /locus_tag="JQG95_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY5889663.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JQG95_003202"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JQG95_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JQG95_003203"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JQG95_003203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY5889664.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /locus_tag="JQG95_003204"
     CDS             3808..5076
                     /locus_tag="JQG95_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY5889665.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JQG95_003205"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="JQG95_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY5889666.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JQG95_003206"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="JQG95_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY5889667.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHSIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="JQG95_003207"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="JQG95_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY5889668.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="JQG95_003208"
     CDS             complement(8331..9863)
                     /locus_tag="JQG95_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY5889669.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /locus_tag="JQG95_003209"
     CDS             10193..11491
                     /locus_tag="JQG95_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY5889670.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..>12023
                     /gene="alr"
                     /locus_tag="JQG95_003210"
     CDS             11501..>12023
                     /gene="alr"
                     /locus_tag="JQG95_003210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY5889671.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMA"
BASE COUNT         3097 a         2852 c         3063 g         3011 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcttgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct gcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagacc actgtatata
     3361 aaaacagtat aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca acaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat aggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagaatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa ccccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgtccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttcatagaat cgatcgtcgc catacacgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg agtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcactc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttaggcttcc agtccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgc
//
LOCUS       DADLCQ010000119        11961 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000119 DADLCQ010000000
VERSION     DADLCQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.119"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..692
                     /locus_tag="JQG95_003211"
     CDS             <1..692
                     /locus_tag="JQG95_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY5889672.1"
                     /translation="AFSLAIYHAHQRHVFGNPLIQQPLMRHVLSRMALQLEGQTALLF
                     RLARAWDRRADAKEALWARLFTPAAKFVICKRGMPFVAEAMEVLGGIGYCEESELPRL
                     YREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQ
                     QLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQND
                     LLLRATGGVCV"
     gene            complement(809..1084)
                     /gene="yjfN"
                     /locus_tag="JQG95_003212"
     CDS             complement(809..1084)
                     /gene="yjfN"
                     /locus_tag="JQG95_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY5889673.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(1233..1562)
                     /gene="bsmA"
                     /locus_tag="JQG95_003213"
     CDS             complement(1233..1562)
                     /gene="bsmA"
                     /locus_tag="JQG95_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY5889674.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1744..2493
                     /gene="yjfP"
                     /locus_tag="JQG95_003214"
     CDS             1744..2493
                     /gene="yjfP"
                     /locus_tag="JQG95_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY5889675.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2490..3245)
                     /gene="ulaR"
                     /locus_tag="JQG95_003215"
     CDS             complement(2490..3245)
                     /gene="ulaR"
                     /locus_tag="JQG95_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY5889676.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3353..4417)
                     /gene="ulaG"
                     /locus_tag="JQG95_003216"
     CDS             complement(3353..4417)
                     /gene="ulaG"
                     /locus_tag="JQG95_003216"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY5889677.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4772..6169
                     /gene="ulaA"
                     /locus_tag="JQG95_003217"
     CDS             4772..6169
                     /gene="ulaA"
                     /locus_tag="JQG95_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY5889678.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            6185..6490
                     /gene="ulaB"
                     /locus_tag="JQG95_003218"
     CDS             6185..6490
                     /gene="ulaB"
                     /locus_tag="JQG95_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY5889679.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6500..6964
                     /gene="ulaC"
                     /locus_tag="JQG95_003219"
     CDS             6500..6964
                     /gene="ulaC"
                     /locus_tag="JQG95_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY5889680.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6978..7628
                     /gene="ulaD"
                     /locus_tag="JQG95_003220"
     CDS             6978..7628
                     /gene="ulaD"
                     /locus_tag="JQG95_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY5889681.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7638..8474
                     /locus_tag="JQG95_003221"
     CDS             7638..8474
                     /locus_tag="JQG95_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY5889682.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            8474..9160
                     /locus_tag="JQG95_003222"
     CDS             8474..9160
                     /locus_tag="JQG95_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY5889683.1"
                     /translation="MQKLKQQVFEANMDLPRYVLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     EIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(9289..9564)
                     /locus_tag="JQG95_003223"
     CDS             complement(9289..9564)
                     /locus_tag="JQG95_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5889684.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9891..10286
                     /gene="rpsF"
                     /locus_tag="JQG95_003224"
     CDS             9891..10286
                     /gene="rpsF"
                     /locus_tag="JQG95_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY5889685.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            10293..10607
                     /gene="priB"
                     /locus_tag="JQG95_003225"
     CDS             10293..10607
                     /gene="priB"
                     /locus_tag="JQG95_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY5889686.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10612..10839
                     /gene="rpsR"
                     /locus_tag="JQG95_003226"
     CDS             10612..10839
                     /gene="rpsR"
                     /locus_tag="JQG95_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY5889687.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10881..11330
                     /gene="rplI"
                     /locus_tag="JQG95_003227"
     CDS             10881..11330
                     /gene="rplI"
                     /locus_tag="JQG95_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY5889688.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11401..11958)
                     /locus_tag="JQG95_003228"
     CDS             complement(11401..11958)
                     /locus_tag="JQG95_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY5889689.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         2818 a         2940 c         3172 g         3031 t
ORIGIN      
        1 gtgcattttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
       61 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
      121 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg tgccgatgcc aaagaagccc
      181 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
      241 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
      301 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
      361 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
      421 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
      481 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
      541 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
      601 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
      661 tgctgcgggc gacgggggga gtgtgtgtgt aagcgtatac gactgatgcg acgctggttt
      721 cgattaacta aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
      781 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
      841 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
      901 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
      961 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     1021 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     1081 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     1141 aacgcccaag cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     1201 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     1261 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     1321 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     1381 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     1441 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     1501 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     1561 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     1621 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     1681 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     1741 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     1801 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     1861 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     1921 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     1981 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     2041 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     2101 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     2161 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     2221 gcacagcaga atgaattcaa taaaattgtc gcgccgctgg cagagtggga agcgacaaac
     2281 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     2341 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     2401 ctgctaacct gttcatggca gccaggcgtg cgtcaccgta ttacgcctga ggcgttagat
     2461 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     2521 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgatcagcat atcgatttga
     2581 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     2641 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     2701 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     2761 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     2821 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     2881 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     2941 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     3001 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     3061 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgct ttcgccaagt
     3121 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     3181 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     3241 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agccaggaaa
     3301 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     3361 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     3421 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     3481 acggcttgaa gccatacttc agacgatctt ttttgatctc ccacagcacg cggatctctt
     3541 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     3601 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     3661 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     3721 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     3781 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     3841 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     3901 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     3961 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     4021 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     4081 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     4141 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     4201 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     4261 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     4321 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     4381 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     4441 caattcgaat caacatgtga ttggttttga ttaatcctga cactattcct tcaggaaggc
     4501 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     4561 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     4621 tgaatcacac tctatccgtg tggaattatt tgcgggtcgc gccacattta atcataaata
     4681 atcttgttgt gattactttt gaaaattaga gtgagtacac aacattccgg gtgtgcggaa
     4741 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     4801 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     4861 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     4921 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     4981 ggcgaaaatg tccgaagtct acggcattaa cggcgcaatt tccgatactt acgcgtcgat
     5041 gatggcaacc atcgaccgca tgggcgatgc ctatagctgg gtaggttacg cggtgttgtt
     5101 agcgctggcg ctgaacatct gttacgtgct gctgcgtcgc attaccggta ttcgtaccat
     5161 catgctgact ggtcatatca tgttccagca ggcggggctg attgccgtta cgttgtttat
     5221 tttcggctac tccatgtgga caaccattat ctgcacggcg attctggttt cgctctactg
     5281 gggcattacc tccaacatga tgtacaagcc gactcaggaa gtgacggacg gctgcggttt
     5341 ctccatcggt caccagcaac agtttgcatc attaattgcc tataaagttg cgccgttcct
     5401 cggcaaaaaa gaggaaagcg ttgaagacct caaactgcca ggctggctga atattttcca
     5461 cgacaacatc gtctccacgg cgattgtcat gaccatcttc tttggtgcca ttctgctctc
     5521 cttcggtatc gacaccgtgc aggcgatggc aggcaaagtg cactggacgg tctatatcct
     5581 gcaaaccggt ttttccttcg cggtggcgat cttcatcatc actcagggtg tgcgcatgtt
     5641 tgtggcggaa ctctctgaag cgtttaacgg tatctcccag cgcctgatcc ctggtgcggt
     5701 tctggcgatt gactgtgcgg ctatttatag cttcgctccg aacgccgtgg tctggggctt
     5761 tatgtggggc accatcggtc agctgattgc ggttggcatc ctggtcgcct gcggttcctc
     5821 gatcctgatt atcccaggct ttatcccgat gttcttctcc aacgccacca tcggcgtgtt
     5881 cgctaaccac ttcggcggct ggcgtgcggc gctgaagatt tgtctggtga tggggatgat
     5941 cgaaatcttt ggttgcgtct gggtggtgaa acttaccggt atgagtgcct ggatggggat
     6001 ggcggactgg tcgattctgg caccgccgat gatgcagggc ttcttctcca tcggtatcgc
     6061 ctttatggcc gtcatcattg taattgcact ggcttatatg ttcttcgctg gccgcgcact
     6121 gcgcgcagaa gaagatgcag aaaaacaatt ggcagaacag tctgcttaat aaggagtttt
     6181 gattatgacc gtacgtattc tggctgtgtg tggcaacgga caaggcagtt ccatgatcat
     6241 gaagatgaaa gtggaccagt ttttaaccca atcaaacatt gaccacacgg taaatagctg
     6301 cgcggttggc gaatacaaaa gcgagttgag cggcgcggat atcatcatcg cttctacgca
     6361 cattgcgggc gaaatcaccg tgaccggcaa caaatacgtg gttggcgtgc gcaacatgct
     6421 ctctcctgcc gactttggcc cgaaactgct ggaagtgatc aaagcgcatt tcccgcagga
     6481 tgtgaagtaa ggacgcgcca tgaaattaca tgattcgctg gcggagaata aatccatccg
     6541 cctgcaggct gaagctgaga catggcagga cgcagtaaaa atcggcgttg acctgctggt
     6601 ggcggcggat gttgtcgagc cgcgttacta ccaggcgatt ctggatgcgg ttgaacagca
     6661 cggtccgtac tttgtgctcg caccaggcct ggcaatgccg cacggtcgcc cggaagaggg
     6721 cgttaagaaa accggttttg cgctggtgac gctgaaaaag ccgctggagt tcaaccacga
     6781 agataacgac ccggtggata tcctcatcac catggcggcg gtcgatgcca acacgcacca
     6841 ggaagtgggc atcatgcaga tcgtcaacct gtttgaagat gaagaaaatt tcgaccgttt
     6901 acgtgcctgc cgtaccgagc aggaagtact ggatctcatt gaccgcacca acgcggcagc
     6961 ttaagaagga attgaagatg tcattaccga tgttgcaagt cgcgctggac aaccagacta
     7021 tggatagcgc ctacgaaacc actcgcctga ttgccgaaga agtcgacatt atcgaagtgg
     7081 gtaccattct gtgcgtgggc gaaggcgtgc gtgcggttcg cgatctgaaa gcgctctacc
     7141 cgcacaaaat cgtgctggca gacgccaaaa ttgccgatgc gggcaaaatt ctttctcgta
     7201 tgtgcttcga agccaacgct gactgggtga ccgtgatttg ttgtgcggat atcaacaccg
     7261 ccaaaggtgc gctggacgta gcaaaagagt ttaacggcga cgtgcagatc gaactgaccg
     7321 gttactggac ctgggaacag gcgcaacagt ggcgcgacgc aggtattcag caggtggttt
     7381 atcaccgcag ccgtgacgcg caggccgctg gcgtggcgtg gggtgaagcc gatatcactg
     7441 cgatcaaacg tctttccgat atgggcttca aagtcaccgt caccggtggc ctggcgctgg
     7501 aagatctgcc gctgttcaaa ggcattccaa ttcatgtgtt tatcgccggt cgcagcatcc
     7561 gtgatgccgc ttctccggtt gaagccgcgc gtcagttcaa acgttccatc gccgaactgt
     7621 ggggctaagg agcggatatg ttgtccaaac aaatcccgct tggcatctat gaaaaagcgc
     7681 tccccgccgg ggagtgctgg ctggaacgcc tgcgactggc aaaaacgttg ggcttcgatt
     7741 ttgtcgagat gtcggtagat gaaactaacg agcgcctgtc gcgcctcgac tggagccgcg
     7801 aacagcgtct ggcgctggtg aatgctattg ttgaaaccgg cgtgcgtgtg ccgtccatgt
     7861 gcctttctgc ccatcgtcgt ttcccgttgg gcagcgaaga tgacgcggtg cgggcgcagg
     7921 ggctggagat tatgcgtaaa gccatccagt tcgcccagga cgttggcatt cgcgtgatcc
     7981 agctggcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt cgtcgtttcc
     8041 gtgacggcct gaaagagagc gttgagatgg cgagccgcgc gcaggtgacg ctggcgatgg
     8101 agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac gcgcactatc
     8161 tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg tgggacaacg
     8221 atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat gtgaaagaca
     8281 ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat ttcgaacgtt
     8341 gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag atgtggagcg
     8401 aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa gcaggcatgg
     8461 tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca tggatctgcc
     8521 gcgctatgtg ctggtgacct ttacttgggg caacgtcagc gctatcgacc gcgaacgcgg
     8581 gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag acgatatggt
     8641 ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt ccgacactgc
     8701 gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc atacccactc
     8761 cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag gcaccacgca
     8821 cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag aagaggtgca
     8881 gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca acgccgagcc
     8941 gctgcatacg ccggaaattg tggtgtatca gcacggaccg ttcgcctggg ggaaagatgc
     9001 ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg cgtggattgc
     9061 gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata aacacttcat
     9121 gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct gcggaaattt
     9181 ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa gccccctgcg
     9241 aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct agcgataaat
     9301 atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa ttacgtggta
     9361 gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt cgctttcagt
     9421 ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca tatttctggc
     9481 ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa gcacagccag
     9541 aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg tgtgctctcc
     9601 tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg gtgaccattt
     9661 ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa atcagcggtt
     9721 tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt tagcggggta
     9781 tgccagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt gcctccccgg
     9841 gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg atgcgtcatt
     9901 acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc atgatcgagc
     9961 gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa gactggggcc
    10021 gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt ctgatgaacg
    10081 ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc aacgatgccg
    10141 ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct ccgatggtta
    10201 aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc gctgatgatg
    10261 ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg ttgtccggca
    10321 ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac tgccagttcg
    10381 tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca tggtgtcaaa
    10441 tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata acggtcggca
    10501 gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga ctgagcaaaa
    10561 tggttttgca tgccgagcag attgaattga tagattctgg agactagcca tatggcacgt
    10621 tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga gatcgactat
    10681 aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt cccaagccgt
    10741 atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa acgcgctcgc
    10801 tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt ccattaatac
    10861 gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca aacctgggta
    10921 gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg gtaccgcagg
    10981 gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt cgcgctgaac
    11041 tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag aaaatcaatg
    11101 cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg ttcggttcca
    11161 tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg gctaagagcg
    11221 aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg agcttccagg
    11281 ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa ttcgttattc
    11341 aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt tcgtcaggca
    11401 ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag gatggtttgc
    11461 tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc gctcgaaacc
    11521 taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt tcaaatcatg
    11581 gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg cattagaaac
    11641 gcctgtaaca gtgtgaagtt ggtcgatata aagatcgatt gttggtagtt cagatgacca
    11701 gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc cggtatagtc
    11761 tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac tgttgggttc
    11821 gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg cacccgcgta
    11881 agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt tccatccaaa
    11941 agaattatca ccattcattg t
//
LOCUS       DADLCQ010000120        11665 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000120 DADLCQ010000000
VERSION     DADLCQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.120"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            597..794
                     /locus_tag="JQG95_003229"
     CDS             597..794
                     /locus_tag="JQG95_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889690.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JQG95_003230"
     CDS             complement(744..1937)
                     /locus_tag="JQG95_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5889691.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JQG95_003231"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JQG95_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY5889692.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JQG95_003232"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JQG95_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY5889693.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JQG95_003233"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JQG95_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY5889694.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="JQG95_003234"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="JQG95_003234"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY5889695.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="JQG95_003235"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="JQG95_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY5889696.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10966..11665
                     /locus_tag="JQG95_003236"
                     /pseudo
     CDS             10966..11665
                     /locus_tag="JQG95_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2960 a         2898 c         2993 g         2814 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgctcagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctgccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattaa
    10801 cgatcaatta gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt
    10861 tacaatatag aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt
    10921 aattcttaat actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac
    10981 tcccttaaat atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga
    11041 gtttctggta aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt
    11101 acagtaaatg cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt
    11161 gcacaaaata aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac
    11221 ggagataaat tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt
    11281 ggcgcagcta ccgcaataag aggttcttat agtgttactg ttgcacataa caaaaagaac
    11341 cctcaggccg cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga
    11401 aattccaatg attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc
    11461 accccggcag agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc
    11521 acttctgacg gttcaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta
    11581 ttaatggtga atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta
    11641 gtctatttga ggtcacccaa tggga
//
LOCUS       DADLCQ010000121        11566 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000121 DADLCQ010000000
VERSION     DADLCQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.121"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(217..292)
                     /locus_tag="JQG95_003237"
     tRNA            complement(217..292)
                     /locus_tag="JQG95_003237"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="JQG95_003238"
     CDS             851..3103
                     /locus_tag="JQG95_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889697.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4416
                     /gene="csiD"
                     /locus_tag="JQG95_003239"
     CDS             3439..4416
                     /gene="csiD"
                     /locus_tag="JQG95_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY5889698.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4436..5704
                     /gene="lhgO"
                     /locus_tag="JQG95_003240"
     CDS             4436..5704
                     /gene="lhgO"
                     /locus_tag="JQG95_003240"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY5889699.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5727..7175
                     /gene="gabD"
                     /locus_tag="JQG95_003241"
                     /pseudo
     CDS             5727..7175
                     /gene="gabD"
                     /locus_tag="JQG95_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7183..8469
                     /gene="gabT"
                     /locus_tag="JQG95_003242"
     CDS             7183..8469
                     /gene="gabT"
                     /locus_tag="JQG95_003242"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY5889700.1"
                     /translation="MRRNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGRE
                     YLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGD
                     FAKKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGEYHGRTHYTLALTGKVNPYSAG
                     MGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYA
                     ASPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGF
                     PLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLK
                     DGLLAIAEKHPEIGDVRGLGSMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSC
                     GPYYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            8707..10107
                     /gene="gabP"
                     /locus_tag="JQG95_003243"
     CDS             8707..10107
                     /gene="gabP"
                     /locus_tag="JQG95_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY5889701.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVISFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10128..10790
                     /gene="csiR"
                     /locus_tag="JQG95_003244"
     CDS             10128..10790
                     /gene="csiR"
                     /locus_tag="JQG95_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY5889702.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10791..11240)
                     /gene="kbp"
                     /locus_tag="JQG95_003245"
     CDS             complement(10791..11240)
                     /gene="kbp"
                     /locus_tag="JQG95_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY5889703.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11324..11482)
                     /locus_tag="JQG95_003246"
     CDS             complement(11324..11482)
                     /locus_tag="JQG95_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY5889704.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2973 a         2840 c         2885 g         2868 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacacc
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 gagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggtccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgagca aaacatgcag ggtggaaatt cgttgctgct gcatctcgat
     4021 gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc gatgcgcttt
     4081 gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt cgacgttgat
     4141 caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa agattttgaa
     4201 gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg cattctttct
     4261 gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct gcacggtcgc
     4321 gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg tggctatttc
     4381 gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg agcggatgta
     4441 tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc aactgattga
     4501 tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca
     4561 gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg gcagcctgaa
     4621 ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc aaaacggcat
     4681 tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa tggaacggat
     4741 gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga taaacgccgt
     4801 ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg tgccgtccag
     4861 cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc aggccagagg
     4921 cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa acggcgtggt
     4981 gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct gttccgggct
     5041 gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta tctgcccgtt
     5101 ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc acctgattta
     5161 ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca tgatcgacgg
     5221 cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg
     5281 cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc gccgggtgct
     5341 gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca aaagcggcta
     5401 tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc agccctggcc
     5461 cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg attttctgtt
     5521 tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag cgacatcagc
     5581 aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa
     5641 ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg catttaatca
     5701 ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta ttccgccagc
     5761 aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc gacgtcacca
     5821 atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat gaaacccgcg
     5881 ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc gccaaagaac
     5941 gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac gatttagcgc
     6001 gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa atcagctacg
     6061 ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc tacaccattc
     6121 ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc gtcaccgccg
     6181 ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt ccggcgctgg
     6241 cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct gcgctggcgc
     6301 tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg gtcaccggtt
     6361 cggcaggcgc ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa ctgtcgttta
     6421 ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag
     6481 tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc gatctcgata
     6541 aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg
     6601 ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa ttacagcagg
     6661 cggtgagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc gggccgctga
     6721 tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg gagaaaggtg
     6781 cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc cagccaacca
     6841 ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc ggccccctcg
     6901 ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat gacaccgagt
     6961 ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc gtcggcgagg
     7021 cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa gtggccccat
     7081 tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt
     7141 acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag gaacagcaat
     7201 aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca aattcacccg
     7261 attttcgctg accgcgcgga aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt
     7321 gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa ggtggtggcc
     7381 gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct ggcttacgag
     7441 ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt cgccaagaaa
     7501 acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat cgcccgcgcc
     7561 gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg cacgcattac
     7621 accctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct gatgccgggt
     7681 catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga tgacgctatc
     7741 gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc cgccatcatg
     7801 attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt tatgcagcgt
     7861 ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt gcagagcggc
     7921 gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc ggatcttacc
     7981 acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg gcgcgcggaa
     8041 gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa cccgattgcc
     8101 tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct gcaaaaagcc
     8161 aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa acacccggag
     8221 atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga agatggcgat
     8281 cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg cgataagggc
     8341 ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt accactcacc
     8401 attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt tgatgaggcg
     8461 aagcagtagc accgccccca tgcaggaggc gacgctgcgc gtcttgtccg gcctacgaga
     8521 atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa caggaataaa
     8581 tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat aacaattaaa
     8641 cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga
     8701 ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa atcacgccac
     8761 gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc
     8821 gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg attactggtg
     8881 gttatgatta tgcggatgct ggcggaaatg gcagttgcta cgcccgatac cggttcgttt
     8941 tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg ctggttgtac
     9001 tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat gatcctgcac
     9061 tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct cgccttaact
     9121 ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc
     9181 aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac
     9241 ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac
     9301 ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa
     9361 attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt ccgcgccacc
     9421 aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca
     9481 ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa
     9541 ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc
     9601 agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc
     9661 ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg
     9721 ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc gcctgcgaag
     9781 gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc
     9841 gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga aattcgcttg
     9901 cgaatgtggc ttgatccctg gctcacctgg ctggtcatca gctttattac ctttgtgttg
     9961 gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg
    10021 atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa
    10081 aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt
    10141 ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg
    10201 aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg ccgttgcagg
    10261 aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc
    10321 gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc gccaatatgg
    10381 aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag gcagacgttc
    10441 tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg
    10501 atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc
    10561 actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat cgatttatct
    10621 ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat cagcaccaga
    10681 ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta atgcgccagc
    10741 atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc
    10801 ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc
    10861 gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc
    10921 agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc
    10981 acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc
    11041 ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg
    11101 ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc
    11161 tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc ggcatcttta
    11221 acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag
    11281 cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca
    11341 cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa
    11401 tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga atgatggtga
    11461 tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa
    11521 gagattttat ctcttaagcg tagtaatttt agcggagcct gccagt
//
LOCUS       DADLCQ010000122        11315 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000122 DADLCQ010000000
VERSION     DADLCQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.122"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..209)
                     /locus_tag="JQG95_003247"
     CDS             complement(<1..209)
                     /locus_tag="JQG95_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5889705.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQG95_003248"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JQG95_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY5889706.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQG95_003249"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JQG95_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY5889707.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQG95_003250"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JQG95_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY5889708.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQG95_003251"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JQG95_003251"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY5889709.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQG95_003252"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JQG95_003252"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY5889710.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDNLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQG95_003253"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JQG95_003253"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY5889711.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JQG95_003254"
     CDS             complement(4598..5320)
                     /locus_tag="JQG95_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5889712.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQG95_003255"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JQG95_003255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5889713.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JQG95_003256"
     CDS             6520..6738
                     /locus_tag="JQG95_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY5889714.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JQG95_003257"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JQG95_003257"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY5889715.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JQG95_003258"
     CDS             complement(7472..7672)
                     /locus_tag="JQG95_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY5889716.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JQG95_003259"
     CDS             complement(7682..9370)
                     /gene="kefB"
                     /locus_tag="JQG95_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY5889717.1"
                     /translation="MGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFI
                     IGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGGIGFAMSST
                     AMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDWMKIGMK
                     VLEFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMALGTF
                     IAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVVLV
                     AVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMAPLLM
                     KLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLER
                     DISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHF
                     PHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLH
                     FRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JQG95_003260"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="JQG95_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY5889718.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..>11315
                     /locus_tag="JQG95_003261"
     CDS             10101..>11315
                     /locus_tag="JQG95_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5889719.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQE"
BASE COUNT         2784 a         3095 c         2718 g         2718 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag attgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggag
//
LOCUS       DADLCQ010000123        11303 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000123 DADLCQ010000000
VERSION     DADLCQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.123"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="JQG95_003262"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="JQG95_003262"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY5889720.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVEPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="JQG95_003263"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="JQG95_003263"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY5889721.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /locus_tag="JQG95_003264"
     CDS             complement(4495..5796)
                     /locus_tag="JQG95_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY5889722.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEINGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="JQG95_003265"
     CDS             6038..6658
                     /locus_tag="JQG95_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY5889723.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="JQG95_003266"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="JQG95_003266"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY5889724.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="JQG95_003267"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="JQG95_003267"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY5889725.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="JQG95_003268"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="JQG95_003268"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY5889726.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="JQG95_003269"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="JQG95_003269"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY5889727.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="JQG95_003270"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="JQG95_003270"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
BASE COUNT         2653 a         3251 c         2781 g         2618 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggctc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcagcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccactg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccattgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaagg
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct atgccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac agg
//
LOCUS       DADLCQ010000124        11175 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000124 DADLCQ010000000
VERSION     DADLCQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.124"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="JQG95_003271"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="JQG95_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY5889728.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="JQG95_003272"
     CDS             696..914
                     /locus_tag="JQG95_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY5889729.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="JQG95_003273"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="JQG95_003273"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY5889730.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="JQG95_003274"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="JQG95_003274"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY5889731.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="JQG95_003275"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="JQG95_003275"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY5889732.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="JQG95_003276"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="JQG95_003276"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY5889733.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="JQG95_003277"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="JQG95_003277"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY5889734.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="JQG95_003278"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="JQG95_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY5889735.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="JQG95_003279"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="JQG95_003279"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY5889736.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            9128..10276
                     /gene="carA"
                     /locus_tag="JQG95_003280"
     CDS             9128..10276
                     /gene="carA"
                     /locus_tag="JQG95_003280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY5889737.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            10294..>11175
                     /gene="carB"
                     /locus_tag="JQG95_003281"
     CDS             10294..>11175
                     /gene="carB"
                     /locus_tag="JQG95_003281"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HAY5889738.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGR"
BASE COUNT         2607 a         2773 c         3104 g         2691 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 ccctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tggtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat gatgcaaaag
     8701 taacacattt aatttattaa ttataaaggg ctttaatttt tagccctttt atttttggtg
     8761 ttatgctttt aaattttcta taactgccaa aaattacatg tttcgtcttc tatttttgtt
     8821 gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt atttcatgca
     8881 atcttcttgc tgcgcaagcg ttttccagaa caggttagat gatgtttttg tcgcttaatg
     8941 cctgtaaaac atgcatgagc cacaaaataa tataaaaaat cctgccatta agttgacttt
     9001 tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa gcagatttgc
     9061 attgatttac gtcatcactg tgaattaata tgcaaataaa gtgagtgagt attctctgga
     9121 gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt ttcacggtcg
     9181 ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt caatgaccgg
     9241 ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc ttacttatcc
     9301 ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg tacatgcaca
     9361 aggtctggtg attcgcgacc tgccgctgat tgccagcaac ttccgtaata ccgaagacct
     9421 ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata cccgtaagct
     9481 gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag gcgataaccc
     9541 ggatgcggcg ctggcgttag aaaaagcccg cgcgttccca ggtctgaacg gcatggatct
     9601 ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct ggacgttgac
     9661 cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg tggcttatga
     9721 ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc gcctgaccat
     9781 cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg gcatcttcct
     9841 ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca tccagaagtt
     9901 cctcgaaacc gatattccgg tattcggcat ctgtctcggt catcagctgc tggcgctggc
     9961 gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc atccggttaa
    10021 agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg cggtggatga
    10081 agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg gtacgttaca
    10141 gggcattcat cgcatcgata agccggcgtt cagcttccag ggtcaccctg aagccagccc
    10201 tggtccacac gacgccgcgc cgttgttcga ccactttatc aagttaattg agcagtaccg
    10261 taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga tataaaaagt
    10321 atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt tgactactct
    10381 ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct ggtgaactcc
    10441 aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat cgagccgatt
    10501 cactgggaag tggtacgcaa gattattgaa aaagagcgcc cggacgcggt gctgccaacc
    10561 atgggcggtc agacggcgct gaactgcgcg ctggagctgg aacgtcaggg cgtgttggaa
    10621 gagttcggtg tcaccatgat tggtgccact gccgatgcga ttgataaagc agaagaccgc
    10681 cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc cggtatcgca
    10741 cacacgatgg aagaagcgct ggctgttgcc gctgacgtgg gcttcccgtg cattattcgc
    10801 ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgtga agagtttgaa
    10861 gaaatttgcg cccgcggtct ggatctctct ccgaccaaag agttgctgat tgatgagtcg
    10921 ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga caactgcatc
    10981 atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc acaccggtga ctccatcact
    11041 gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa cgcctcgatg
    11101 gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt tgcggtgaac
    11161 ccgaaaaacg gtcgc
//
LOCUS       DADLCQ010000125        11084 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000125 DADLCQ010000000
VERSION     DADLCQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.125"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="JQG95_003282"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="JQG95_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY5889739.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="JQG95_003283"
     CDS             complement(2051..2974)
                     /locus_tag="JQG95_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5889740.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="JQG95_003284"
     CDS             3191..4534
                     /locus_tag="JQG95_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5889741.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMTPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /locus_tag="JQG95_003285"
     CDS             4759..6414
                     /locus_tag="JQG95_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY5889742.1"
                     /translation="MDRRRFIKGLMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="JQG95_003286"
     CDS             6554..6778
                     /locus_tag="JQG95_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY5889743.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="JQG95_003287"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="JQG95_003287"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY5889744.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="JQG95_003288"
     CDS             complement(7372..8352)
                     /locus_tag="JQG95_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889745.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="JQG95_003289"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="JQG95_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY5889746.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="JQG95_003290"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="JQG95_003290"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY5889747.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11029
                     /locus_tag="JQG95_003291"
     CDS             10361..11029
                     /locus_tag="JQG95_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY5889748.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         2863 a         2588 c         2787 g         2846 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaataatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgacgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggtttaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcaggcatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tattctggtt ttttaaacaa ttactctgat ttaaaagaaa caacctcagc
    10501 aacaggtaaa cctgttttac gttgggtaga cccgagtttt gatcaaagca aatatgacag
    10561 catcgtctgg aacccaatca cttattatcc ggtaccaaaa ccttctaccc aggtagggca
    10621 gaaagttctg gataaaattt tgaactatac caacaccgaa atgaaagaag cgatagcgca
    10681 gcgtaaacca gtggttacca ccgctgggcc gcgtagtctg attttccgtg gggccattac
    10741 cggtgtagat accagcaaag aagggctgca attctatgaa gtggttcctg ttgcattagt
    10801 ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg gatactcgcc tctattttga
    10861 aggtgagctg attgatgcag cgactaataa accggttatc aaagtcgttc gtcagggcga
    10921 aggtaaagac ctgaataacg aaagtacgcc aatggctttc gaaaatatta aacaagttat
    10981 tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg
    11041 ccatcgttag atggcgcttt ttatccggtg cgccgtaaaa cccc
//
LOCUS       DADLCQ010000126        11065 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000126 DADLCQ010000000
VERSION     DADLCQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.126"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1482)
                     /locus_tag="JQG95_003292"
     CDS             complement(<1..1482)
                     /locus_tag="JQG95_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY5889749.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLS"
     gene            complement(1416..2039)
                     /locus_tag="JQG95_003293"
     CDS             complement(1416..2039)
                     /locus_tag="JQG95_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY5889750.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2036..3724)
                     /locus_tag="JQG95_003294"
                     /pseudo
     CDS             complement(2036..3724)
                     /locus_tag="JQG95_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(3873..6500)
                     /gene="gyrA"
                     /locus_tag="JQG95_003295"
     CDS             complement(3873..6500)
                     /gene="gyrA"
                     /locus_tag="JQG95_003295"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY5889751.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6647..7369
                     /locus_tag="JQG95_003296"
     CDS             6647..7369
                     /locus_tag="JQG95_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY5889752.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7497..9428)
                     /gene="yfaL"
                     /locus_tag="JQG95_003297"
     CDS             complement(7497..9428)
                     /gene="yfaL"
                     /locus_tag="JQG95_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAY5889753.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEV
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(9441..11036)
                     /locus_tag="JQG95_003298"
     CDS             complement(9441..11036)
                     /locus_tag="JQG95_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HAY5889754.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2561 a         3083 c         2715 g         2706 t
ORIGIN      
        1 tgataattgc tgagcgcgca aacttaactg cacgcgggca ttttttaccg gctcgccatc
       61 cgggtagagc agttgcagtt tgccgctgac cgcttcgcca gttttgaact cttttttgtc
      121 gagagctaaa ccaatctcga aatgtggctt gatgtagttt gccacgcgaa aactgctgct
      181 atagacctga ttgcggtaag caagacgtaa ctcataacct ccggcaacgg cattttctgg
      241 caggcggaaa cttccctgcc cgccattgcg cgcatccagc gtgacatcga cggtttgcaa
      301 cagactgccg ttagcgtcca gcaccgaaag cttcgccggg gcgctgacga tgggggatga
      361 atgcaacgga tcgcggaact cgcggcccat cactttaaca tcgacacgat cgcctgcgcg
      421 atatagcggg cgatcggtaa aaatatacaa gcgggtgttg tagatttcgc tttcgtagaa
      481 gaagttctcg gagacaaaaa cgccgccttc agcatcctta cccagaatgt atgaacgttc
      541 tggcgatata tgttgtaact gcaaagtacc gctgtcatcg gtcaccccgc gggtcatcac
      601 gccaagaccg tcagtccaca agatctctga gccgggcttc gcttcaccct gttttttacc
      661 cgcggtccat accagaagct cgttacctga cactttgcta agcgccacgg tatcggaaac
      721 aaacaccacc gtcgtcgccc gatacccacc taccatcgcc tcgacgaggt acagccccgg
      781 ctcttgttgg ccgagaggaa tataaatatt acccggctgc ggcgagatga aattgctgga
      841 tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt ttagcctgcc atagtggata
      901 acggaactgt tccaccaagg gatatttttt cagcggggaa aactggttgt tctgtacata
      961 acgggaaggt ttaataatgg cattgccgag atgtaattcg ggtaatgcct gagtcacatt
     1021 ctgccgtgac tgagaagaga aagtacgctg catcacgcgg cgagatttgc cgtaccagtt
     1081 atcccacagc caggtcagcg tattgttcag cccgtcgccc agatattgcg gttgcaccac
     1141 aatgcgatgc aggtttttct gctggcgcaa aaatgccatc gggtcaggaa tacgatatag
     1201 gcgaacgtcc acgccgccgt actcttccat ctgatagcgc cgataatcac gccccggcgc
     1261 ttccagtcgc actttcgcct cttcactgct gctaaaactg ctgtcagcaa gcaaaaagaa
     1321 tgttcccccg gcgggcggcg catagttact ggaaggaagc gaatcatcag ctttagcaag
     1381 ccctgttccc acaagagaca gggacaagca cgctgctatc gcgccagaaa atttaaacga
     1441 taaatgccaa tgaaattggg attggaatcg ttgggtatcc atcgggtgtc cttccatgtc
     1501 ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag ttttcgtggc gcttccggtg
     1561 tggtagatga cgtaacgccc catccagacc attaagtgct gggcatcgcc ctgatcgaaa
     1621 aaaatcatat cgccaggcag cgcctggttt atgtcttggc caataaactg gctgttgtac
     1681 tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc cgctcccctg attccagttt
     1741 tgcgccagtt gacgctgttc aggtgttagc gtcatctctg gcggcaaata ctggctagat
     1801 aaaccgttac tttttagcca tttactgtcg tgaactttca gcgtctcgtt cgccgcaaat
     1861 cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac ttggcccctg gcggagctgt
     1921 tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg attgttcgac attcagcatt
     1981 tcgctatggg caacaacaca acacagccag caaatcagcg ccagcagccc gtgtctcata
     2041 gcggctgcca ggttatgggt agccactgcc aggcgacacc gggttccatc tgggccagct
     2101 tcatgacata acgcggttgt tgagataaag cgtccagctt cggcattaat aaagtttgtg
     2161 cggcgttata aaaaaccggt tcgagattct tcggcagact ggtcaacgtt tcgttacgca
     2221 gcagtttcgc cacgccttgt ggattgatat agagcggaac gatgccatca gtgggtatta
     2281 catccaccat tgccgggcgg gttttattca gtgtttgcag ggcgttatta accagcgtgt
     2341 catcgaggga gaaaagcagc gttttgtttt gcatcgccag cgacacacgg aaaaaataat
     2401 ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg atactgaccg tatcgggaac
     2461 tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt ctggcttacc ggcaaaacac
     2521 cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt aaacagtttc cctggcaatt
     2581 gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag cggggtttgc aattttgagt
     2641 cttcatacca gcacagcccc gcggcaccgt ctaacgcccc attcagcttg tcgttttcct
     2701 ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga atacggcacc gccacacaga
     2761 agctggctcc ggcaggcata ctgttccaga ccggcgtaaa atcgaaactg gcatctacgc
     2821 tggcggattc atcatttaac gccacaaaac tgtgccagcc gtcgttaccc atttcgaagc
     2881 gtacgccagc aaaagaaggc attaaccgct ggtagccaaa acccagcaac ctggcgctga
     2941 ccacgatgcg caggcgtact ggcgtttttt cggctgtacg ctcttccagg ccaaagctgg
     3001 cttgccagcg ttttttaccg ctcaacaaat cacttgcgat cgccgtggct tcggtatcct
     3061 gctgatcatc tttaaacaac atatccgtac tggaaaacac cagcatcttg tcctgatatg
     3121 tcgcgaacat cagggcgtta ttgccgttat agcgcaactg ataaacagga atagtttcac
     3181 tatttatctt gatgctactg atttccgttt tgcttaacta gctgtcgctg gtagcggcaa
     3241 acaacaatgg ctccagcagt ttgcttaaac cactgcgctg gatcagcacc atgtaatgtg
     3301 aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc gggctgatct aagagtgacg
     3361 aaaagagctt atctttcagc gtgagatcgt gttcatagac aatgcggcga atgctgcctt
     3421 caatgcccag acgatcggca tgattctgat aatagaaaac gaaatcttcg ctcagaacat
     3481 cgtggagaaa cggaatggtg aggagatctt tgggaagctg gctcagagag tcgctgtcta
     3541 gaaagaggtc cggctcattg agatcgattt gcagattgtt gtgcaccacc agcggcgaca
     3601 acgttttttc tggcccactg ccagcatatt gcaacgccca gacgccagcg gaaagcagtg
     3661 ctattgcgcc aaaacctaca agaccataga accgccagcc tttcgccttt ttttcaccac
     3721 tcattgccac attccttgtg tatagccagc cattttttac gggcacagcc aaactttacc
     3781 gcgccctaat acgacaaaag cccagacttt gcagcctgga cttttcaatt caaacaaggg
     3841 agatagctcc cttttggcat gaagaagtaa aattattctt cttctggctc gtcgtcaacg
     3901 tccacttccg gagcaatttc atcatcccct tccgcggcac tgccgtcgat ggtatccaga
     3961 tcttcctcgt caaccggttc agcaacacgc tgcagaccca ctacgttttc atcttccgca
     4021 gtacggatga ggatcacgcc ctgggtgtta cggcccacga tgctgatttc tgaaacgcga
     4081 gtacgtacca gcgtaccggc atcggtgatc atcatgatct ggtcgcagtc atctacctgc
     4141 accgcgccaa ccactaaacc gttacgttcg gtaaccttga tggagataac ccctttcgtc
     4201 gcacgcgact tggttgggta ttccgccact gcagtacgtt taccgtaacc gttttgcgtt
     4261 gcggtgagga ttgcgccatc gccacgaggc acgatcagag agacgacttt atcgccttca
     4321 cctaagcgaa taccgcgcac accagtggta ttgcagccca tcgcacggac agaagactct
     4381 ttaaagcgca ccactttacc ttcggcggag aacagcatta cttcatcttc gccactggtc
     4441 agatcaacgc cgatcagctc atcgccgtca accagtttga tcgccacttt accggcggta
     4501 cgcagacggt tgaattcggt gaggacggtt ttcttcacag taccgttagc ggtcgccatg
     4561 aagactttca cgccttcttc aaactcggtc accggcagga tcgcggtgat acgttcgtcc
     4621 tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg cgccacgagt ggcttccggc
     4681 aactggtaaa ctttcatcga atagacgcga ccacggctgg agaagcacag aatatggtcg
     4741 tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt ctttaatacg tgcggcagat
     4801 ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa gcggctgata tttaacgtag
     4861 ccctggtgag agagcgtcac gaccacatct tcctgggtga tcagatcttc caggttgatg
     4921 tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac cgaactgttc acgaaccagc
     4981 tccagctctt cgcggatcac ttccatcaga cgatcggcgc taccaagaat acgcaacagt
     5041 tccgcgatct gatccagcag ctctttgtat tcgtcgagca gtttttcgtg ctcaaggccg
     5101 gtcagtttct gcaaacgcag atccagaatc gcctgagctt gctgttcggt caggtagtac
     5161 agaccatcac gcacgccgaa ctcaggctcc agccattccg gacgcgcagc atcgtcgcca
     5221 gcacgttcca acatggcggc aacgttgccc agctgccacg gattagcaac cagcgcagtt
     5281 ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga tgatcgggtc aatgttcgcc
     5341 agcgccacgg ctaatgcttc aaggatatga gcacgatcgc gagctttacg cagttcgaaa
     5401 atagtacgac gggtcaccac ttcacggcgg tgacgaacaa acgccgcgat gatgtctttc
     5461 aggttcatga tcttcggctg accatggtgc aatgccacca tgttgatacc gaaagaaacc
     5521 tgcaactggg tctgggagta gaggttgttg agcacaactt caccgaccgc atcgcgtttc
     5581 acttcaatca cgatgcgcat accgtctttg tcagactcgt cacgcagcgc gctgatgcct
     5641 tccacgcgtt tttcttttac cagttccgca atcttctcga tcaggcgcgc tttgtttacc
     5701 tgatacggaa tttcgtggac gataatggtt tcacgtccgg ttttggcgtc aacttccact
     5761 tcagcacggg cgcggatata taccttgccg cgaccggtac ggtaagcttc ttcaataccg
     5821 cgacgaccgt taatgattgc cgccgtcggg aagtccggcc ccgggatgtg ttccatcagc
     5881 ccttcaatgc tgatgtcttc atcatcgata tacgccagac aaccgttgat gacttccgtc
     5941 aggttgtgcg gcgggatgtt ggttgccata cctacggcga taccggaaga accgttcacc
     6001 agcaggttag gaattttggt tggcatgacg tcgggaattt tttccgtgcc gtcatagtta
     6061 tcaacgaaat cgaccgtctc tttttcgaga tcggccatca gttcatgggc aattttcgcc
     6121 agacggattt ccgtataacg cattgccgcc gcagagtcgc cgtcgatgga accgaagtta
     6181 ccctgaccgt ctaccagcat gtaacgcagc gagaatggct gcgccatacg gacgatcgtg
     6241 tcataaaccg ccgagtcacc atggggatgg tatttaccga ttacgtcacc aacgacacgg
     6301 gcagattttt tataggcttt gttccagtca ttgcctagta cgttcatggc gtaaagtacg
     6361 cgacggtgta ccggcttcag gccatctcgg acatctggca gcgcacggcc aacaatgacc
     6421 gacatcgcat aatccagata ggagctcttt agctcttcct caatgttgac cggtgtaatt
     6481 tctctcgcaa ggtcgctcat ctaaccgcta tccctctact gtatcccgga ttcaaaggtc
     6541 gcaaattata acacagccgc gcagtttgag gtaaacctat acgctttatt cacatccaat
     6601 gcctgatata ctcgtttgtc ttgccaatta cggagtagaa gtgccaatga atgccgaaaa
     6661 atcgccggaa aaccataacg tagaccacga agagatcgct aaatttgaag ccgtcgcctc
     6721 ccgctggtgg gatcttgaag gtgagttcaa accgttgcac cgcattaacc cgctgcgtct
     6781 gggctatatt gccgagcgtg ctggcggttt atttggcaaa aaggtgctcg atgtcggttg
     6841 tggcggcggc attctggccg agagtatggc gcgcgaaggc gcgacggtga ccggtctgga
     6901 tatgggcttt gagccattgc aggtggcaaa gctgcacgcg ctggaaagcg gcattcaggt
     6961 ggattacgtg caggaaaccg tggaagagca cgcggcaaaa catgccgggc agtatgatgt
     7021 ggtgacctgc atggagatgc tggagcacgt acccgatccg cagtcagtgg tcagagcctg
     7081 tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg acgcttaacc gcaacggcaa
     7141 gtcatggctg atggcggtgg ttggcgcgga atatattttg cgcatggtgc ccaaaggtac
     7201 gcatgatgtg aagaagttta ttaaaccggc agagctgctt ggctgggtgg atcagaccag
     7261 tttaaaagag cgacatataa ccgggctaca ttacaacccg atcactaatt cctttaagct
     7321 cggccccggc gtggatgtga actatatgct gcacacgcag aataagtgag gttgatgttt
     7381 ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat caggcctaca aatgctctcc
     7441 gtaggccaga taaggcgttt acgccgcatc cggcaattgt gatggtcaga cagcgattac
     7501 catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa aatcatcact ccctttctgc
     7561 cacgccacgc tgccgcgcag ggacactcgc tgactgatat tgcccgtgac tcccactttt
     7621 atttcacccc gtagcttcac cgcatcgtca ctgatagtgc tgccatcttc ttcaatttcc
     7681 gtcgcatggg gatcgtgata gtaattcaga tctaatgttg gtgtgacacc aaccgcggta
     7741 cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat ccccctgcga ttgtgacacg
     7801 cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc
     7861 tgcggttcaa tcaccacacc acgccccggt aaccactgat acccgacttc cagcgaggcg
     7921 ataatccccg acgaatggta acgatccacg ccatcttcat gttcggaaac atcattgtta
     7981 aaccacgcgt attgcagcca gctatccagc caggccccct gcttctgatt accgtgctga
     8041 taccagcttg aggtcagccc aacggcataa ccgtggttct ggttatcggc gcgagttccg
     8101 gtcatattcg agcggctgtc gccctgatta tcactgtagc caccaaccgc cccaagcatc
     8161 cactcgccat ccgtgcccca gcgcccgcta aacaagccgc cgctaagctg caccgtagaa
     8221 gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat gataacgtcc gccgataaca
     8281 cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc gtcgctccat cataaacgcc
     8341 tgatttgccg cccgcaggtt attgagataa ccgccaactt tggcattcag caccggctgg
     8401 taagcaggcg taggggccgg gtcgggggtt gggtcaggat ccggcgtggg atcaggatcg
     8461 ggagtcgggt ctggctcagg tggcgatggc ggcgttactt cttgcgatcg cagataccag
     8521 tcgttgttat cttccactag cgtgtagtca tacgctccca tgttgacgta gccactgcca
     8581 gccagactga actgtgcatt gttttgaaac tgcgtgggat cagctgcgaa atcaaccact
     8641 ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg agttaaccac cacagttgtg
     8701 ttgccagcag tattaccgtt catcaccaat tgatcgctta ccgagtcatc gccgtttaat
     8761 tcgctgtcga atagtagcgt accgccgccg gtataatcac cgtttaccgt cagcgtatcg
     8821 ccagcgacgc cattttgcag gctaaccgca ccggacgtat tggtcagatc accattaacc
     8881 gtcacgtcag caagataggt caggctgtca gtttcaaggt tggtattaag ctcgcctgtc
     8941 aacgtcacgc catcggtagc atttaccagc gtcccatcgc cgttaaatag tgaggcatta
     9001 agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg taccatcgct gatgtcgata
     9061 gtgccgctgg aagtagcatc aattgactga atatcctgat ccgcgccagt aacgaacgtt
     9121 gccccatcac caacccacag tgacgaagca taaggaaaga tatccgcaac atcgcctttc
     9181 agggtgcctt cttcgacacg taccgccgac tgcgttgtac cgctggcggt cagctccagc
     9241 gtacccgccc ccgttttagt aaatgtgcta ccctcatcct gatgctgccc gctgctgtca
     9301 gccatcagtg cgccccactg cgtgtctacc cccgcatcaa ctgccacttc accgtcggcg
     9361 cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc gagtatcgac attggcagta
     9421 atctccatca cgctttcttg ttacttatcg gtaaagataa cctggcggtt atagtgggta
     9481 tctccaagct gcgagttgtc gctcaccatc agcgtaccgg aggcgatttg tgttgtgccc
     9541 aaataactat tattgtttgc cagacttacc tgcccgtcgc tcgcatcgac caccacgtag
     9601 acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc cacctgcaag atctaccaca
     9661 tcctgactgc cgataaccgt tccgctgctg ccactgactt caaggccatc attgtatgat
     9721 gtgccgctct gccaagtgga gaaatcagag agatcgagca caccgccgtt taacacgatc
     9781 gactgcggat cgtcctggag agcggtaaga tctgccgcgt cgccttccag cgaaagcacc
     9841 gcaccatcat caacgactat atcgccggtt agcgccatcg actgcgcccc tgccagcacg
     9901 tagctgccgt tttgcgcaat ggtgagctga ccagcacctt cgatgatgcc agcaaaactg
     9961 ccctgattaa cagtaacgtt gccaccagca tcgatattta aagtgccatt ctgaaagccc
    10021 gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt ttagctcagc ctgattctga
    10081 tactgatcaa tactccctat cgtcagaccg tagcagtctt gcggatcgtc ctggcaatgc
    10141 gtatcgccga cattcatcag ggagttgctg cgacccaggg taacttcacc gttttcaatc
    10201 tgcatctcac cagtaaagtc attgttatct gcattaagta ccagatcgcc ggaacctgtt
    10261 ttggtgatta acccggtacc agcaatagag tcaacagctc cgtcattctc tgtattgccg
    10321 ataaccagcg tttttccgtc ggcaatatca aaggtaactt cacttaagcc gagatacata
    10381 aagccacccg ccgcagtgga gggaccatct ccatagcctg ctgcgctatt gttctcatcg
    10441 actaacacgc cgccgttctg gctgtagctg tcatcaacag aaatatcaat aagataggga
    10501 gccgtcgcgc tattggtata tatcgccccg ccataacctt cggctgtgtt atttgtaaag
    10561 gcggtgttat ttattatcgt ataacctgaa ggatgcttgc tgtcgctatt attatcggta
    10621 acatcgattg ccccgccatc gccatcactg taacttgttg atgtatatgc ctggttattg
    10681 ttaaaaacat catcacttaa atagacgtca ttattaatgg tataaattgc gccacctttg
    10741 ccatcattag cgatattatt gatggcgtta gtgacacgta aatcgacggc accagtatca
    10801 ttagtaccag aagaatagat tgcgccacca tagccgcctg cgacgttacc ggaaaaaata
    10861 acatcagtaa gattcagcgt tgagttttct ttagcaaata tggccccgcc gttattatat
    10921 tctcctgtaa cggtgttatt ggcaaacaga gtcattccag tttcattttc tggtaatagt
    10981 gaaaattccg ctccttgttg caaaaatacg gccccaccgc tggcattatt ggtcatatcc
    11041 gaaaaaacca gccattgccc atcgg
//
LOCUS       DADLCQ010000127        10926 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000127 DADLCQ010000000
VERSION     DADLCQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.127"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            154..504
                     /locus_tag="JQG95_003299"
     CDS             154..504
                     /locus_tag="JQG95_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY5889755.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="JQG95_003300"
     CDS             557..952
                     /locus_tag="JQG95_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889756.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="JQG95_003301"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="JQG95_003301"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY5889757.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="JQG95_003302"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="JQG95_003302"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY5889758.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /locus_tag="JQG95_003303"
     CDS             complement(2869..5298)
                     /locus_tag="JQG95_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY5889759.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="JQG95_003304"
     CDS             complement(5323..6423)
                     /locus_tag="JQG95_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY5889760.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="JQG95_003305"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="JQG95_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY5889761.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="JQG95_003306"
     CDS             complement(7571..8137)
                     /locus_tag="JQG95_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY5889762.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="JQG95_003307"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="JQG95_003307"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY5889763.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="JQG95_003308"
     CDS             10263..10751
                     /locus_tag="JQG95_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY5889764.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2670 a         2788 c         2605 g         2863 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 tatagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgc
//
LOCUS       DADLCQ010000128        10925 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000128 DADLCQ010000000
VERSION     DADLCQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.128"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..203
                     /locus_tag="JQG95_003309"
     CDS             <1..203
                     /locus_tag="JQG95_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889765.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="JQG95_003310"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="JQG95_003310"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY5889766.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="JQG95_003311"
     CDS             2102..2818
                     /locus_tag="JQG95_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY5889767.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="JQG95_003312"
     tRNA            complement(3274..3349)
                     /locus_tag="JQG95_003312"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="JQG95_003313"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="JQG95_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="JQG95_003314"
     tRNA            5082..5157
                     /locus_tag="JQG95_003314"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="JQG95_003315"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="JQG95_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY5889768.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLEFCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="JQG95_003316"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="JQG95_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY5889769.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKIIYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="JQG95_003317"
     tRNA            7492..7567
                     /locus_tag="JQG95_003317"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="JQG95_003318"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="JQG95_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY5889770.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="JQG95_003319"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="JQG95_003319"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY5889771.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="JQG95_003320"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="JQG95_003320"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY5889772.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="JQG95_003321"
     CDS             complement(10451..>10925)
                     /gene="cobU"
                     /locus_tag="JQG95_003321"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY5889773.1"
                     /translation="SQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELI
                     HADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRC
                     PAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3035 a         2782 c         2364 g         2744 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcaggc
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaaatt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagataattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgctggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccgggtgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ctcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaa
//
LOCUS       DADLCQ010000129        10894 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000129 DADLCQ010000000
VERSION     DADLCQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.129"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(152..874)
                     /gene="baeR"
                     /locus_tag="JQG95_003322"
     CDS             complement(152..874)
                     /gene="baeR"
                     /locus_tag="JQG95_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY5889774.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(871..2274)
                     /gene="baeS"
                     /locus_tag="JQG95_003323"
     CDS             complement(871..2274)
                     /gene="baeS"
                     /locus_tag="JQG95_003323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY5889775.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2271..3686)
                     /locus_tag="JQG95_003324"
     CDS             complement(2271..3686)
                     /locus_tag="JQG95_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5889776.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="JQG95_003325"
     CDS             complement(3687..6752)
                     /gene="mdtC"
                     /locus_tag="JQG95_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY5889777.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="JQG95_003326"
                     /pseudo
     CDS             complement(6753..9875)
                     /gene="mdtB"
                     /locus_tag="JQG95_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="JQG95_003327"
     CDS             complement(9875..>10894)
                     /gene="mdtA"
                     /locus_tag="JQG95_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY5889778.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQSATTPEEKATSREYAK
                     KGARS"
BASE COUNT         2507 a         3070 c         3000 g         2317 t
ORIGIN      
        1 ctaaaggtgc tgtttactga ttaaagtatg agcgattttc tgatgcctgc cagggcggag
       61 agataatgtc agtttgctga ccatgcgtgg aataatgttg ccggatcggg tctgaacgcc
      121 atctccggct aacaaagtaa tgtcgctaaa actaaacgat gcggcaggcg tcggcttccc
      181 agcggtaacc aacgccgtaa acggcgcgga taaatgactg ttcggcgtcg agagattcca
      241 gcttgcggcg cagattttta atgtggctgt caatggtgcg gtcggttact acgcggtagt
      301 cgtcataaag atgattgagc aattgctcgc gggagaacac ttttcctggt tcgtgagaga
      361 gcgttttaag cagacgaaat tccgcaggcg tcaggtcaag cattttaccg cgccatgaag
      421 cctgaaaacg accttcgtcg ataatcaacg ggctttcggc atcctgttgc tgaagttcgc
      481 gttgcggttt acaacggcgc aaaatggttt tgacacgcgc taccacttcc cgtgggctgt
      541 acggcttgca gatgtagtca tcggcgccaa tctccagccc cagcaggcgg tcgatctctt
      601 cgatttttgc cgtcaccatc acgatcggaa catcggaaaa acgacgaatt tcccggcata
      661 atgtcaggcc atcggtgcca gggagcatca gatccaacag gatcagatcc ggtggtgtct
      721 ggcgcacata cgccagtacc tgatcgccgt ggctgataag cgtcggcgca tagctcgcag
      781 cacgcagata atcaatgagc aactgcccca gcttcggttc atcttccacg atcaaaatac
      841 gcggtgtgtt ttcgtcgatt ggtaactcgg tcatacttct ctctgtaaat cccgttccag
      901 cggtaactct actgtaatgc ttaccccgcc aaaaggcgaa tgggcggcaa taatgcggcc
      961 attatgggct tcaacaatgt tcaggcaaat cgccagcccc agcccggaac cgccgctggc
     1021 gcggttgcgg gaaccttcgg tgcgataaaa acgttcaaac aatttttgta gctgatcgtc
     1081 actgacacct ggcgcactgt cggcaaaggt caggcgcacc gttttgtcgt gctgctcggc
     1141 agagattttc aggctgccgc cgctgtcagt gtagcgcagg ctgttttcca gtaagttatt
     1201 gaataactgc attaaacggt cgcgatcgcc aaatacggtg atactgtctg gcagggaaaa
     1261 ttgcagtttc aggccacggc tggcgaatcg ttcgcgaaat gcgccgcccg ccacttccag
     1321 cagtgggatc aaatctaccg gtgctttttg ataggcgaga gcgccttcat cagacatcga
     1381 caactgatga agatcgtcaa ccagtttggt cagtgtaccg acctccgcct gtaaagacgc
     1441 caccgtctcc ggcgtgaatt tgcgcacgcc atcctgaatg gcttccagtt caccgcgcag
     1501 caccgctaat ggcgtacgca gttcgtgaga aatatcggcc ataaaatcgc gccgcatttg
     1561 ctggtttttc tccagcgtgc tggcgagctg gttgaagtct tgcgccagtt tgcccagttc
     1621 atcttcactg gtgggcgtta cgcgggtagt gaaatcgccc gccgccagtt tgtgcgtgcc
     1681 atcgacaagt cgttttaccg gtgccagcaa accgcgcgcc agcagaaagg tggcgagcgc
     1741 cgcgagtaac gttgccaggg cgacgatcag ccagctggtt tgccgctgtt gtttatcgaa
     1801 attgatatca gtattgcggg tcagccgctc aacaggggag gcaatcaccg cgcccacttc
     1861 cgcaccgttg accagaatgg gtcgccgtgt accgtcaggt ggaatcggcg ctcgcggacc
     1921 aaccagtact ttgttgtttt gatcgaccac ccagaactgg gtacgccagc cgtgcggcgg
     1981 catacccggg ccgggtttat cttccgaatt atcgtgttca aatgaacgca ggatctgaaa
     2041 gacaaagcga tcgttgttgc gcaggaagcg ccagttacca tgctgcgcat actgctcgcc
     2101 aagcgcatca cttaacagct gtaatcgctg ttcattgccg tgtttgatgt aatcaataaa
     2161 gccacgctca aaactgatac gcaccgccca gtgcatactg atcagcaaga caatgcaggt
     2221 ggcgaaaatc gccagaaaca gtttgccggt aataccgggt cgccagaact tcattgcgcg
     2281 ctcctttttc gccgcgaaat agctacattt tgatgcgtat cgttcggcac tctggcaaag
     2341 atgaacgccg gaagggcgat gataaacgcc atgctaagcc aggtgtacat aaagacggtt
     2401 tgtgtggtgc cgctgtcgac gctgatatgc tgcgaaccaa aaagccccag caacagcccg
     2461 gcgatggtga cgccgatact catcgacaat tgcataatca tcgacagcag gctgtttccg
     2521 ctgctcgcca gattgtccgg gagatctttc agcgtcaggg tgttcatgga ggagaaacgg
     2581 gtcgagttga ccatcccttg taaaaacagg acgaacggca aaacgtagta ccagcccaac
     2641 agagcggtag tcataaacaa cagggtgacc agcgacagac ccagcgtggt cgctaccagt
     2701 acccgacgat aaccaaagcg attcaccacc tgtaccacaa ttcgcttcat tcccatgctg
     2761 ccaagcacca tcgggatcat catcagtccg gcatgaaacg gcgagaaacc gaggccaatt
     2821 tgcaggaaaa ccggtgtcat aaagggcaac atgccactgc caatacgtcc ggcaaagctc
     2881 cccgccaggc ccagcgaaaa ggtacgagtg cggaacagtt tcagactgaa cagggcgcgg
     2941 ttgttatttc tggcgtgcag cagataaagc accagtgcca ccacgccaac tgcgaccagg
     3001 ccagcaatcg ccagcggcga taaacctgta cctttactgc cgtccagcgc cagggttaat
     3061 accgccatgc caaccgccag caataaaaag ccggaaagat caaagcgccg cgtctgcatg
     3121 gtgtagttcg gcattaacat caatgtggcg atcgcaccga taatccccac cggaatgttg
     3181 atcaaaaaga tccagtgcca cgacgcgtat tctaccagca gaccgccgag cgccggaccg
     3241 agcagcggac cgacctgacc gggtaacgtg acaaaggtca tcgccgccat atattgctcg
     3301 cgcggtacga ttttcatcac cgtcaacctg ccgaccggca ccatcatcgc accgccaacg
     3361 ccctgtaacg cgcgtgccag caacagttcg ttcagcgtgc cggaaagcgc gcaaaacagt
     3421 gaaccgagag taaacagcac gatggcggta aagaaaatat tgcgcacgcc gactttgtcc
     3481 gccagccagc cgctggcggg cagcatcacc gccacggtca gcacataaga gacaatgacc
     3541 atgtgcatat gcaacggact ttccccgagg ctttgcgcca ttgagggaag ggcggtgttt
     3601 acgatggtgg tgtccagcga ctgcataaag aagccgaaag ccacaatcca taattgccag
     3661 cgggtgctgt cgggaagatc tgtcatttac tcggttaccg tttgtttagg tttacgcgaa
     3721 aaacgcagcc gcagacggtc gaaaaagaga tacaccaccg gcgtggtata cagcgtcagg
     3781 agctggctca tcaccagacc gccgacaatg gtgatctcca ggggttgccg cagctccgag
     3841 ccgtcaccgc ccgacaatac cagcggcagc gcgccaaaca gcgccgccag ggtggtcatc
     3901 ataatcgggc gaaaacgcag cagacaggcc tggaaaatag cttcctgcgg cgtcaggtta
     3961 ccgtgccgtt gggcttcaag cgcaaaatcg accatcataa tggcgttttt cttcacgatg
     4021 ccgattaata gcatgatccc tatcagggcg attaggctga acggggcatt gaacagctcc
     4081 agcgccaaca gcgctccaac gcccgccgag ggcagggtgg agagaatcgt cagcggatgt
     4141 acgtaactct cgtaaaggat acccagcacg atatacaccg tggcgatggc ggcgataatc
     4201 aggatcacct gcgagttcat cgtctcctgg aaaacctgcg ccgtgccggc aaaactgccg
     4261 cgcaccgtcg aaggcacacc aagctgggtc attgcgcgat cgatcgccgc actggcgtcc
     4321 gagagcgatt ttccggtcgg caggttgaac gagatggtcg aggccgccga taatccctga
     4381 tgattcaccg atagtggggc attcgccggt tgccatttag cgaaatacga cagcgggatc
     4441 gctttgcctt cgttattgat aacgaacatt ttttccagcg cactgatgtc ctgggtatag
     4501 cgcggatcca cttccatcac cactttatac tggttcatcg gctggtaaat ggtcgagatt
     4561 tgccgctgac cgaaggcgtt atttaacaga ctgttggcgg cctgtacgtc gattcccagc
     4621 cgtgccatgg tgtcgcggtc gtaaaccaga ttcatctccg cgccgttatc ctgctgatcg
     4681 gagttcacgt ccgccagttc cggcaacgtc gccagttttt tgcggatttt cggctcccat
     4741 tcgcgcagtg ccgccaggtc gtcggataac aacgtgtact ggtagctggc gttcgactga
     4801 cgcccaccaa cgcgaatatc ttgtaccgcc atcaggaaca gattcgcccc cggttctttc
     4861 gccagcttca cgcgcagacg gtcgataatt tgctgcgccg tttcgctgcg ttcgtcgcgt
     4921 ggcttgaggg tgataaacat catcccactg ttaactcgtg aaccgccggt aaagccggtg
     4981 acattatcca ctgccggatc gtcacggata attttcatga aatcctgcaa tttgccgcgc
     5041 atcgcctgaa acgaaatact ctgatccgcc tgtatcccac ccatcagtac gccggtgtcc
     5101 tgctccggga agaaggtttt cgggatcgag atattcagcg caatggtgcc aagcagcacc
     5161 acgcccacca gacgggtatg attgagcacc catttcagcg acttgccgta gccttgttgc
     5221 agggctacca acatgcggcc aaaaccacgc agtcgctttt gctcacgcgg cttgctggct
     5281 tttagcatcc agccgcacat cattggcgtt agcgtcaggg aaaccagcaa cgaaatacca
     5341 atggcgacag aaagcgtcac ggcaaactcg cgtaacagtc ggcccggcaa tccgcccatc
     5401 aacagcagcg gcaggaacac cgccaccagt gacagactca tcgacagcac cgtaaagccg
     5461 acttcgcgag taccttgcag cgcggcctgt agtggtttca tccccgcttc cagatgacgg
     5521 gcaatgtttt ccagcaccac aatggcgtca tccaccacga aaccagtagc aatggtgagc
     5581 gccatcagcg aaagattatt gagactgaat ccgcacaggt acatcgccgc aaacgtacca
     5641 atcagcgaaa ccggcaccgc aacggcggga ataatagtgg cgcgacccga gcgcaggaat
     5701 aaaaagacca ccagaatcac cagcgccacc gagataatca gcgtttgctc gacttcttcc
     5761 agcgaggcgc gaatggtggg ggagcgatcc tgggcaattt gcagatcaat cgccgccgga
     5821 atggtctcct gcaactctgg taattttgcc cggatgctgt ctaccgtctg gataatattg
     5881 gcttccggca gtttgcggat catcagtaaa atagccggtt tggcgttggt catcccggcg
     5941 ttgcgcacat cctgcactga gtcagtcacc gtcgccacat cgcccagacg aaccgcgccg
     6001 ccgttgttgt agtgaataat cagcggctga tattcagcgg cggttttcag ttcatcgttg
     6061 gtctggatct gccagcggtg agtgccatct tccagcgccc cctgcggttt acgcacgttg
     6121 gcattgctga tggcagtgcg tacgtcgtcc agcgacacgc cctgattaaa cagcgcctgc
     6181 ggggtcagcc cgacgcgcac ggcgggcagc gagctgccac cgacatcgac atcaccgaca
     6241 ccgtcgattt gcgaaatcgt cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc
     6301 tgcgaataag tatcggatgt cagcgtgagg atcataattg gcgcatccga cgggttcgct
     6361 ttgcgatagg tcgggcggct gggcatcccg ctgggcagca aactttgtgc agcgttgatc
     6421 gccgcctgca catcacgcgc tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata
     6481 atgcgcgtgc tgccgagcga actgctggag gtcatttcac taaccccggc aatgcgccca
     6541 agtgagcgct ccagcggcgt ggcaacggaa gacgccattg tctccggcga cgcaccgggc
     6601 agcgaggcgc tgaccataat caccggaaaa tcgacctgcg gcagcggggc gaccggcagc
     6661 atacggaaac ccagtatgcc gcacagggta atggcgaccg acagtaaaat cgtcgccacc
     6721 gggcggtaaa tgaagagggc aaaaaacttc acttacgcct cctcttcatg gcgagcaaag
     6781 cgacttttgg tccacaatgc caggcggtcg aacagcagat aaatcaccgg cgtggtgaac
     6841 agcgtcagca cctggctgac aatcagaccg ccgaccatgc cgatacctaa cggacgacgc
     6901 agttccgcgc cgaccccggt actcaacatc agcggcagcg cgccaagcag agccgccagg
     6961 gtggtcatca ggatcggacg aaaacgcaac agacaagcct ggtagattgc atcgcgcggc
     7021 gacatgcctt gctcgcgctc agcagccagc gcgaagtcga tcatcatgat ggcgttcttc
     7081 ttcacaatac cgatcagcaa aataatgccg ataatcgcaa tcacatccag ttcgctaccg
     7141 gcaatcatta atgccagcag cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg
     7201 atcgggtgaa taaagctctc gtacagaatg ccgagcacga tatacatcgc caccaccgcc
     7261 gcgacaatca gccagacagt gctgcccagc gccgactgga aggcgagggt gctgccctag
     7321 aattgcgtgg tgatatccac cggcagattc agcgtctttt cggtgtccat aatcgcctgc
     7381 accgcatcgc ccagcgaata gttatccggc acgttaaagg agatggttgt taccgggaac
     7441 tgatccagat ggttgatgga aagcggcgca aaacgctgct caattttggc aattgagctt
     7501 aacggcacca cgccaccgtc gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct
     7561 ggggtatttt cggtattgtg ctccagcacc acgcgatact ggttagcctg agtataaata
     7621 gtggaaatca gtcgctgacc aaacgcgttg tacagggcgt tatcgacgtc cgccatgctg
     7681 atccccagac ggctggcgct gtcgcgatca acattgacat acgccaccag ccctttgtcc
     7741 tgccagtcgc tggagacatc agaaagctgt ggcagttgct ggagtttttc catcaactgt
     7801 ggcacccagg tactgagcgc atccagtgac gtggcctgca aggtaaactg gtactgggtg
     7861 cggctgacct gagtatcgat ggtcaggtcc tgcgttggtt gcaggaagag atcgacgccc
     7921 ggcactttat ctaccgccgt ttgcagacgg gcgatgactt tttgcacccg atcatcacgt
     7981 tcatccaacg gtttgaggtt gatttgtaaa cgtgcactat tcagcgacgg gttagtgcca
     8041 tcaacgccaa caaatgaggt caggctttgc actgccggat cctgcaaaat cacgtccgcg
     8101 acctggcgtt gtcgctgggc catattggca aaggagctgg attgcggtgc ctgcaaagtg
     8161 ccctgaataa tgccgttgtc ctgtaccggg aagaaacctt tcggaatgaa cacccacagc
     8221 agcacgctaa gcagcagcgt gctgagtgcc acgcttaagg tcagccacgg atgattcagc
     8281 actttcgcca gtccacgacc ataggcggcg attatcctgt cgaacatttt ttccgaggca
     8341 cgggagaagc ggttctgttt acgcaacgac tcctggctga gcatccgcgc gcacatcatc
     8401 ggtgtcaggg tcagcgacac caccgctgag atcaaaatcg ctaccgccag ggtaatagca
     8461 aattcgcgga acagtcgccc gacgatatcg cccataaaca gcagtgggat caacaccgca
     8521 atcagtgaga aggtcagcga gataatggta aagccgattt cacctgcgcc cttgagcgcc
     8581 gccgccaacg gtttttcgcc tttttcgata tagcgggaaa tgttttcgat caccacgatg
     8641 gcgtcatcga ccacgaatcc ggtggcgata gttaacgcca tcagtgtcag gttattgatt
     8701 gaaaaatcga gaaacaccat aaccgcgaaa gtgccgatta acgacagcgg tacagcaaca
     8761 ccgggaatga tggtcgccgg aatattgcgc aaaaacaggt agataatcat gactaccagc
     8821 gcgatagcca tcatcaattc aaactgagta tcatcgacgg atgcgcggat attggtggtg
     8881 cgatcggaaa gcactgtcac cttcaccgat ttcggcagac tctcagtgag ctgtggcagc
     8941 atctgccgaa tgctgtcggc ggtggagata atgttagcac cgggctggcg ctgaacattc
     9001 atcacaatgg cctgttcttt gttcgcccac gcgccgagcc agctgttttc tgcaccttgc
     9061 tctacagttg cgacatcgcc cagacgaatt ggcgcgccgt tctggtaggc gatgattagc
     9121 tggcgatact cttcggcgga ttgcatctgg tcgttcgcgg aaagcgtgac cgcacgggaa
     9181 gggccgtcga ggctaccttt tgccgagtta acgttagcgc cggtaatggc ggtgcgcacg
     9241 gtttcgctgg tcaggccgag ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc
     9301 ggacgctgac cgccggaaag cgtcaccagg ccgacgccgg aaatctgcga gattttctgc
     9361 gcgacgcggg tttccaccat atcttccact tgcgtcatcg gcatggcggt tgaggtgacg
     9421 gcgagcgtca tgatcggcgg atctgccggg ttcactttgc tgtaaaccgg cgggttaggc
     9481 agatcgctcg gcaacaagtt ggtcgcagcg ttaatcgcgg cctgcacttc ctgctcggcg
     9541 acattgagcg gtaatgttag ctggaactgc aaagtgataa ctgacgcacc gccggaactt
     9601 tgcgacgaca tctgtttcag gccagacatc tgcccgaact ggcgttcgag cggcgcggta
     9661 acggcagagg tcatgacatc cgggctggca cctgggtaga gcgtgaccac ctgaatggtc
     9721 ggatagtcca cttccggcag cgccgaaacg ggcagggcgc gataaccgat aatcccggcg
     9781 agtaagatcg ccaccatcag cagcgtggtg gcgacaggac gcataataaa caggcgcgac
     9841 gggccgcctg tgctgctcgg gggtaacacc tgcatcagga gcgtgctcct tttttcgcgt
     9901 attcgcggct ggtggctttc tcttccggag tggtggcgct ctgggcttcc accacttcca
     9961 ctttcgcccc ttcggtcagg cgatcaatgc cgtctgtcac cacgcgatcg cccgcagaaa
    10021 tacctgcacg gatcaccact ttctgactgt cctgaatgcc cggcgtcacc agatgtttgc
    10081 tgaccttgtt ttcgctattc agcacccaga caaaatggcc ttcattgccc atttgcaggg
    10141 cggctgttgg gatcactacg gcgttttgtt cggtgtcgac taacatgcgc gcgttaacaa
    10201 actgattggg aaacagcgca tcatcctgat tattaaagcg tgctttcact ttaatcgtac
    10261 cggttgtggc atcgatttgg ttatcgaggc ttaacagcgt gccttcactt aatttcttcg
    10321 aattggtgcg atcccaggct tctaccatca gcggttttcc ggctttttgc gcctgcacta
    10381 cagtagcgat atcgctttcc ggcagggtaa agactaaatc gataggatgc gtctgggtga
    10441 tcaccacaat tccggtggta tcaccactgg agatttggtt accaacatca acttgcttga
    10501 gaccaacgcg accatcgact ggtgcggtga tgcggctcca gtcgagttgc aactgcgcgc
    10561 tggcgacgct tgcttcatca gccttaatgg tgccttcggt ttcactgacc agcgcctgtt
    10621 gggcatccag ctcctgacgg gagacgagat tggtttttgc cagttgctga taacgcgcca
    10681 gatcacggcg ggcgttagca agcgtggctt tatcttttgc cagttggccc tgggtttgtg
    10741 ctaatgcaac tttgaactgg ctggggtcaa tttctgccag taaatcgcct gctttgacct
    10801 gctggccttc ctggaaatgt aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa
    10861 cagtattagc ggcggtaatg gtgccaagcc cggt
//
LOCUS       DADLCQ010000130        10665 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000130 DADLCQ010000000
VERSION     DADLCQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.130"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(51..1400)
                     /gene="lysC"
                     /locus_tag="JQG95_003328"
     CDS             complement(51..1400)
                     /gene="lysC"
                     /locus_tag="JQG95_003328"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY5889779.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLELGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            1925..3574
                     /gene="pgi"
                     /locus_tag="JQG95_003329"
     CDS             1925..3574
                     /gene="pgi"
                     /locus_tag="JQG95_003329"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY5889780.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4036..4278
                     /locus_tag="JQG95_003330"
     CDS             4036..4278
                     /locus_tag="JQG95_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889781.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTITTTTTSTQ"
     gene            4392..5030
                     /locus_tag="JQG95_003331"
     CDS             4392..5030
                     /locus_tag="JQG95_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY5889782.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5027..5764
                     /locus_tag="JQG95_003332"
     CDS             5027..5764
                     /locus_tag="JQG95_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY5889783.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5764..7860
                     /locus_tag="JQG95_003333"
     CDS             5764..7860
                     /locus_tag="JQG95_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY5889784.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7907..8185)
                     /locus_tag="JQG95_003334"
     CDS             complement(7907..8185)
                     /locus_tag="JQG95_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889785.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8186..8329
                     /locus_tag="JQG95_003335"
     CDS             8186..8329
                     /locus_tag="JQG95_003335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889786.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            8455..8865
                     /gene="psiE"
                     /locus_tag="JQG95_003336"
     CDS             8455..8865
                     /gene="psiE"
                     /locus_tag="JQG95_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY5889787.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="JQG95_003337"
     CDS             complement(8909..10384)
                     /gene="xylE"
                     /locus_tag="JQG95_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY5889788.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         2710 a         2722 c         2763 g         2470 t
ORIGIN      
        1 gacaagaaaa tcaatacggc ccgaaatata gcttccaggc catacagtat ttactcaaac
       61 aaattactat gcagtttttg caccacctgc tcggcatctt cgccgggcac caggaagcac
      121 aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg
      181 aatacctctt tgccaacgcc gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc
      241 gccagacctt cttccacctc cacccggcac agtgcggaaa gctccatcag cagagattgc
      301 gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat caagggttaa tgccacgctc
      361 acttctgacg tggtgattaa gtctaccgaa atattatgcc gcgcgaggat gccgaaaact
      421 tccgcgagga agccgcgaga atgcagcata ttcaggctgt gcaaagtgag cagagtctga
      481 ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt cagttttatt gcacaccagc
      541 gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga ccgggatatc gctgcgtact
      601 gcgggtagca acgttgccgg atgcagtact tttgcaccaa aagttgccat ctctgccgct
      661 tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg
      721 tagatgcccg ggacgtcggt ccagatatca acacgagatg cgtgtaaagc ctccgccagc
      781 aaggctgccg tataatcgct gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg
      841 ctaccgataa atccctgggt gatcactaag ccttcattga ggcgtgggag cagctgcagc
      901 gcggccagtt ccgccagcgc ggctacatct ggctctgcac gaccaaatcg gtcgttggta
      961 cgcatcactt tacgtacatc aaaccactgt gcctgaacat cgcgttcgcg caggatttcg
     1021 acaaacagca gggtcgacat cagctcgccg tggctgacca gctcatctgt cagcgccgga
     1081 gacgttgcca gcgccgccgc ttctgccaga acagtaatgt tctccagcag acgttcaatc
     1141 tcttcacgga taacgttcgg gtaacgcaga cgttccagaa tggcaaactg gatgttgcga
     1201 atagcgtcga gtttttcgaa tcgctcgcca agttccagcc cttccgctaa agcgaccagc
     1261 agattagtga taccagcaga agccgagagg actactaaac gcacgttggc atcagaaagc
     1321 acaatatcag cgctgcggtt catggcgtca aaatcagcta cgctggtacc gccaaatttg
     1381 gagacaacaa tttcagacat aactacctcg tgtcagggga tccattttca gccttggcac
     1441 aagggaagag cggaagacgg gtgggcgcag agcgatactt cgctactatt ttcacccaga
     1501 agtgctccac cacttgcgaa acgcccgact gcgaacgctt ctggtgacaa cccaggggat
     1561 tcagcccctg tagccgatga tgaacgtggc cagccgttca atcacctcgg cgatgcaccc
     1621 cctcaggtgt tatcacagga ctggctcctc caacaccgtt acttgggcaa cgcgcctctt
     1681 ctggcctgcg ctagcgcagg tagtacattt ataaataaag ggtgagcggg gcggttgtca
     1741 acgacggggt catgcggatt tttcatccac tcctggcggt cagtagttca gctaataaat
     1801 gcttcactgc gctaagggtt tacactcaac attacactaa cggcactaaa accatcacat
     1861 ttttctgtga ctggcgctac aatcttccaa agtcacaatt ctcaaaatca gaagagtatt
     1921 gctaatgaaa aacatcaatc caacgcagac cgctgcctgg caggcactac agaaacactt
     1981 cgatgaaatg aaagacgtta cgatcgccga tctttttgct aaagacggcg atcgtttttc
     2041 taagttctcc gcaaccttcg acgatcagat gctggtggat tactccaaaa accgcatcac
     2101 tgaagagacg ctggcgaaat tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat
     2161 taagtcgatg ttctctggcg agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt
     2221 agcgctgcgt aatcgtagca ataccccgat tctggttgat ggcaaagatg ttatgccaga
     2281 agtcaacgcg gtgctggaga agatgaaaac cttctcggaa gcgattatct ccggtgagtg
     2341 gaagggttat accggcaaag caatcactga cgtagtgaac atcgggatcg gcggttctga
     2401 cctcggcccg tacatggtga ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca
     2461 ctttgtttct aacgtcgatg ggactcacat ctcggaagtg ctgaaaaaag taaacccgga
     2521 aaccacgctg ttcctggtag catctaaaac cttcaccact caggaaacca tgaccaacgc
     2581 ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca
     2641 ttttgcggcg ctctcaacca atgccaaagc cgttggcgag tttggtattg atactgccaa
     2701 catgttcgag ttctgggact gggtcggcgg ccgttactct ttgtggtcag cgattggcct
     2761 gtcgattgtt ctctccatcg gctttgataa cttcgttgaa ctgctttctg gcgcacacgc
     2821 aatggacaag catttctcca ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct
     2881 gattggcatc tggtacaaca atttctttgg tgcggaaact gaagcgattc tgccgtatga
     2941 ccagtatatg caccgtttcg cggcgtactt ccagcagggc aatatggagt ccaacggtaa
     3001 gtatgttgac cgtaacggta acgttgtgga ttaccagact ggcccgatta tctggggtga
     3061 accaggcact aacggtcagc acgcgttcta ccagctgatc caccagggaa ccaaaatggt
     3121 accgtgcgat ttcatcgccc cggctatcac ccataacccg ctctctgatc accaccagaa
     3181 actgctgtct aacttcttcg cccagaccga agcgctggcg tttggtaaat cccgcgaagt
     3241 ggttgagcag gaatatcgcg atcagggtaa agatccggca acgcttgact acgtggtgcc
     3301 gttcaaagtg ttcgaaggta accgcccgac caactccatc ctgctgcgcg aaatcactcc
     3361 gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa atctttactc agggcgtgat
     3421 cctgaacatc ttcaccttcg accagtgggg cgtggagctg ggtaaacaac tggcaaaccg
     3481 tattctgcca gagctgaaag atgataaaga aatcagcagc cacgatagct cgaccaatgg
     3541 tctgattaac cgctataaag cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat
     3601 gtaggccgga taaggcgttt acgccgcatc cggcaaccgg agcctgatgc caatatcgac
     3661 attaatgcat agtatgagga gttaaataac cttaaaaaca atgccgacaa taagtcggca
     3721 ttgttttatc agataaatcc ccttgtctgt gatttaacgg aaatcatacc gtgaggttaa
     3781 tcctaaaata gatttttaat cgttgtttat ttcggagaat acgcagatta attacttttg
     3841 tttttatttt aagcttatga tttttattgt tatttaaata taagttgaaa cttatatttg
     3901 atattcattc caattatcct aaaactccat cgctaattcc ccgcgccgta attcgcatgc
     3961 tttagttgtg tatactcgat cccgcccgaa atgtttttgg gtaaatctcc attcattcaa
     4021 tgaagggaaa ttgttatgaa aaaagttctg tatggcattt ttgccatatc tgcgcttgcg
     4081 gcgacttctg cgtgggctgc acctgtacag gtgggcgaag cggcagagtc ggcagcaacg
     4141 tcggtttcgg cggggagttc ctccgcgacc agcgtcagca ccgtaagctc ggcggtgggt
     4201 gtcgcgctcg cggcaaccgg tggcggtgat ggttctaata ccgggaccat cacaaccacg
     4261 accaccagta cccagtaata aagtatgtat ccccaaaata atttgagtca ttgcatctgt
     4321 ggcttgaagt atgaagggat ttaccataac cacactccgg tgtggttatt ctgcccctct
     4381 ggagaagagt cgtgaagcga cctgcactca ttcttatctg cctgctatta caggcctgtt
     4441 cagccacgac taaagagctg ggcaattcac tgtgggacag tctgttcggc acgccaggcg
     4501 tacagctgac ggacgatgat attcaaaata tgccctacgc cagccagtac atgcagctta
     4561 atggcgggcc gcaattgttt gtggtgctgg ccttcgctga agacggacaa caaaaatggg
     4621 tcactcagga tcaagccact cttgtcacac aacatggtcg tctggtgaag actttgcttg
     4681 gcggcgacaa cctgattgaa gtgaataacc ttgccgccga cccgctgatt aaacccgcac
     4741 aaattgttga tggtgcaaca tggacgcgca cgatgggctg gaccgagtac cagcaggtac
     4801 gctacgccac cgcacgctca gtcttcaaat gggatggcac cgataccgtc aaggtcggca
     4861 gcgatgaaac cctggttcgc gtgctggacg aagaagtctc caccgaccag gcgcgctggc
     4921 ataaccgcta ctggatcgac agcgaagggc aaattcgcca gtcggaacag tatctcggcg
     4981 cggattattt cccggtgaaa accactctca tcaaggcggc aaaacaatga ttaaacaaac
     5041 tattgtcgcg ttgcttttga gtgtgggagc gtcatcggtc ttcgcggcag gaaccgtcaa
     5101 ggtgttcagc aatggcagca gtgaggccaa aacgctaacg ggcgcagagc atttaatcga
     5161 tctggtaggg caacctcgac tggcaaacag ctggtggcct ggtgcggtga ttagcgaaga
     5221 gctggcaacg gcggcagcat tgcgtcagca gcaggcgttg ctgacacggc tggcagaaca
     5281 gggcgcagat tccagcaccg acgatgccgc cgccattaac gccttacgcc agcaaatcca
     5341 ggcattgaaa gtgacaggca ggcaaaaaat caatcttgat cccgatatcg tccgcgttgc
     5401 cgaacgcggt aacccgccgt tgcagggcaa ctacacgctg tgggtcggac caccgccgtc
     5461 cacggtcacg ttgttcgggc ttatcagcca tcctggcaat cagccattca ctccaggtcg
     5521 cgacgtggcg agctatctct ctgaccagag cctgctcagc ggtgcggatc gcagctacgc
     5581 gtgggtcgtt tacccggacg gacgcacgca aaaagcgccg gtggcttact ggaacaagcg
     5641 tcacgtagag ccgatgcccg gcagcattat ttatgttggc ctcgcagatt ccgtctggag
     5701 tgagacgcct gatgccctta acgccgacat tcttcagact ctgacgcagc ggatacccca
     5761 ataatgaaaa aaagacatct gcttagctta ctggcgctgg gcattagcac agcttgctac
     5821 ggcgaaacat atcctgcgcc cattggtccg tcgcagtcgg atttcggtgg cgtaggatta
     5881 ttacaaactc ccaccgcgcg tatggcacgg gaaggggagt tgagtctgaa ctatcgcgat
     5941 aacgatcagt accgttatta ctcagcttca gtgcaactct tcccgtggct ggaaacaacg
     6001 ctgcgctaca ccgacgtgcg cacccggcag tacagcagcg tcgaagcgtt ctctggcgat
     6061 caaacgtata aagataaagc cttcgatctc aaactgcgtt tgtgggaaga gagttactgg
     6121 ctgccgcaag tggcggttgg tgcgcgggat atcggcggta cggggctgtt tgatgcggaa
     6181 tatcttgttg ccagcaaagc ctgggggccg ttcgatttta cgctcggcct gggctgggga
     6241 tatctgggca ccagcggtaa tgtaaaaaat ccgctctgtt cagccagtga taaatattgc
     6301 tatcgcgata acagctacaa acaggcggga tctatcgacg gtagccagat gttccacggt
     6361 cctgcctcac tgtttggcgg cgtggaatac cagacgccct ggcaaccgct gcgtctgaaa
     6421 ctggagtatg aaggtaataa ttatcagcag gattttgccg ggaagctgga gcaaaaaagt
     6481 aagtttaacg tcggggcgat ttatcgcgtt accgattggg ccgacgttaa ccttagctat
     6541 gaacgtggca acacctttat gtttggcgtc acgctgcgca ccaactttaa cgatctgcgc
     6601 ccgtcctaca tcgataacgc ccgcccgcaa tatcaaccgc agccgcagga tgccattttg
     6661 cagcattcgg tagtggcgaa tcagttaacg ttgctgaaat acaacgccgg acttgccgat
     6721 ccgcagatcc aggcgaaagg cgatacgctg tatgttaccg gcgagcaggt gaaatatcgt
     6781 gattcgcgcg aagggatcat ccgtgctaat cggatcgtga tgaacgatct gccggatggg
     6841 atcaaaacga tccgcattac ggaaaatcgc cttaacatgc cgcaagtgac gacggaaacc
     6901 gatgtcgcca gcctgaaaaa tcatctcgcc ggagagccgt tgggccacga aacgacgctg
     6961 gcgcacaaac gcgtcgagcc agtggttccg aagtccaccg agcagggctg gtatatcgac
     7021 aaatcacgct ttgatttcca tatcgatccg gtgctgaacc agtcggtcgg tggcccggaa
     7081 aacttttaca tgtatcagct gggcgtgatg ggaacggcag atttgtggct gacggaccat
     7141 ctactgacca ccggcagcct gtttgcaaat cttgccaaca actacgacaa gtttaactac
     7201 accaatccac cgcaggactc gcacttaccg cgcgttcgta cccatgtgcg cgagtatgtg
     7261 cagaacgatg tctatgtgaa taacctgcaa gccaactact tccagcatct gggcaacggc
     7321 ttctacggtc aggtctatgg aggttatctc gaaaccatgt ttggcggtgc gggggccgaa
     7381 gtgttgtatc gtccgctgga cagtaactgg gcgtttggtc tggatgccaa ctacgttaaa
     7441 cagcgcgact ggcgtagtgc aaaagatatg atgaaattca ccgactacag cgtgaaaacc
     7501 ggacatctga ccgcctactg gacgccatct ttcgcccagg acgtgttggt gaaagccagc
     7561 gtcgggcagt atctggcagg ggataaaggc ggcacgctgg agatcgctaa acgctttgac
     7621 agcggcgtgg tggtgggtgg ctatgccacg atcaccaacg tttcgaaaga ggagtacggc
     7681 gaaggggact tcaccaaagg cgtgtatgtc tcggtaccgt tggatctctt ctcgtctggc
     7741 ccgacacgca gccgtgcggc gattggctgg acgccgctga cgcgtgacgg tggtcagcaa
     7801 cttgggcgta agttccagtt gtatgacatg accagcgacc gtagcgtaaa tttccgctaa
     7861 gtcatgggaa aggtgccagt tttcgcactc actggcaccc tcattcttaa tgacctgttg
     7921 agccatcagg aacgcagttc acccccggtt tacgatctgg tgtgtaaagg cgaaaacatt
     7981 ctgtcttcct gtccgtcgat atcggttgca ggtctggttg cctgagcgac tgggccatca
     8041 ttgggctgga gacattgctt tgcggtaggt cggctttatc agcagtaccg agcggaccag
     8101 cataagcagg aagaacagag actgataaca tcaaagcagc aaaataaggc ttcattttta
     8161 ccacctttat caggttacgt ttcatttgtt ccagaggaac attgtcgatt tttcgcgcat
     8221 tgctggtggc tgggaatcac ctgaatgggt gatttttgaa ttaccggctt tggtgcggtt
     8281 tgtcttgccg gatgcgccgc caggcgcggc ttatccggcc tacgggtagg tatatccggc
     8341 tttggtggag gcgcgctcca aatccaggtt gaacaaaaca tacacaaaaa atatagatct
     8401 ccgtcacatt tttgcgttat acaggaagct cgccactgtg aaggaggtac tgctatgacg
     8461 tcactctctc gtccgcgcgt ggagtttatc tccaccattt tgcagaccgt actcaatctt
     8521 ggcctgctgt gcctcggcct gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg
     8581 gctgatgtgc tgttcgcgcc agaacaaacc agcaaatatg agctggtaga aggactggtg
     8641 gtttactttc tctatttcga atttatcgcg ctgattgtga agtactttca gtccggtttt
     8701 cacttcccgc tacgttactt tgtctatatt gggatcaccg ccattgtgcg cttgatcatc
     8761 gtcgatcata aatcgccact ggatgtgttg atctactcgg ctgcgatcct gttactggtg
     8821 atcaccctgt ggctgtgtaa ttcgaaacgg ctgaagcggg agtaaaaaga cagcacgccg
     8881 aaatggcgcg gcgtgctgga caggaagatt acagcgtagc agtttgttgt gttttcttcg
     8941 tttccggttc ccagagcgct tccagctcct caagggtttt acctttggtt tccgggacaa
     9001 atttccacat aaacagtgct gccagaacgc ccatacaacc gtaaatccag taggagaaac
     9061 cgttgtggaa atgggccacc agccaggagt ttttgtccat catcgggaag gtccaggaga
     9121 cgaagtagtt cgccagccac tgggccgcca ccgcgattgc cagcgcttta ccacgaatag
     9181 cattcgggaa gatttccgac agcagtaccc agcataccgg accccaggac atggcaaagg
     9241 cggcaacata gaacagcatc gacagtagcg ccacaatacc cgatgcctga gtgtaaaacg
     9301 cggtaccgag gctaaacata ccgattgcca ttccgagtgc gccgataatt tgcagtggct
     9361 tacgaccaaa tttatccaca gtcataattg ccagtacggt aaaggtgagg ttgataactc
     9421 cgacaataat ggtctgcaac agcgcgatat ccgtgctggc ccccagcgtt ttgaacactt
     9481 ccggcgcgta gtacagcacc acattgatgc cgacaaattg ctggaagatg gagagcataa
     9541 cgccgattac aatcacgccc acgccaaaca tcagcagacg accaccggtt ttgcggccat
     9601 gatccaggga gtgtttaatt tcctgtactg cctgagttgc aagcgtgttg cccataattt
     9661 tgcgcaggat actttccgtc tgttcttgct tgccgcgtga catcagccag cgaggacttt
     9721 ctggcacggt atacagcagc attaagaaca gcagtgcagg gatacattcc gaggcaaaca
     9781 tataacgcct gccgtcagta ttcagccagc tggcatcacc ggaacgggca ataaaatagt
     9841 ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa ctggttaaat gaaaccagtt
     9901 ttccgcgaat atgagctgga gccagttccg caatatacat tggtgaaagc attgaggcta
     9961 aaccaacgcc aataccgcca ataatacgat aaataacaaa ttccgggaca taacctgcca
    10021 gataaatagg tactgtgttg tccgggttta tagaggtaaa accaagttct ggccaggcag
    10081 aacctacacc agaaataaaa aacaggacag cagaaatctt aagtgaatca cgacggccga
    10141 agcggttact gcaataacca ccgagggcac cgccgatgat gcaaccaatc agagcgctgg
    10201 ctacgcaaaa tcctaacagg gagttggcag cggattcact taagttttgt ggagcaacaa
    10261 agacggtatt gagtgactca acagtaccgg aaataacggc ggtgtcgtag ccaaataata
    10321 aaccacctaa tgtagcgact aaggtaatcg aaaatatata actggaatta tactgggtat
    10381 tcattcagac ctgccttaga ccattctgat cttttatcag tgcattgatg ttggaattgt
    10441 caggcagtta aggcaattat cattttttac aatgcgttta cgtaatgtct tttctgtgat
    10501 cttaattgtg ataattatca aaaattgatg aaaacaatac tattgccgtg actcagagca
    10561 cgaaagagaa ttatcgtaag tgggaaaaca aataacgtaa aaataataag ttctatgatg
    10621 ggaatattaa ccggcgaacg attcagattg cagacgaaag aaaaa
//
LOCUS       DADLCQ010000131        10648 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000131 DADLCQ010000000
VERSION     DADLCQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.131"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(24..1052)
                     /locus_tag="JQG95_003338"
     CDS             complement(24..1052)
                     /locus_tag="JQG95_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY5889789.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRQMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1227..2819
                     /locus_tag="JQG95_003339"
     CDS             1227..2819
                     /locus_tag="JQG95_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY5889790.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2829..4001
                     /gene="malY"
                     /locus_tag="JQG95_003340"
     CDS             2829..4001
                     /gene="malY"
                     /locus_tag="JQG95_003340"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY5889791.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4105..5106
                     /gene="add"
                     /locus_tag="JQG95_003341"
     CDS             4105..5106
                     /gene="add"
                     /locus_tag="JQG95_003341"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY5889792.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5142..6182)
                     /locus_tag="JQG95_003342"
     CDS             complement(5142..6182)
                     /locus_tag="JQG95_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY5889793.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKYVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6425..6550
                     /gene="blr"
                     /locus_tag="JQG95_003343"
     CDS             6425..6550
                     /gene="blr"
                     /locus_tag="JQG95_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY5889794.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6823..7038
                     /gene="ydgT"
                     /locus_tag="JQG95_003344"
     CDS             6823..7038
                     /gene="ydgT"
                     /locus_tag="JQG95_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY5889795.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7124..7564
                     /locus_tag="JQG95_003345"
     CDS             7124..7564
                     /locus_tag="JQG95_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY5889796.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7641..8222
                     /gene="rsxA"
                     /locus_tag="JQG95_003346"
     CDS             7641..8222
                     /gene="rsxA"
                     /locus_tag="JQG95_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY5889797.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8222..8800
                     /gene="rsxB"
                     /locus_tag="JQG95_003347"
     CDS             8222..8800
                     /gene="rsxB"
                     /locus_tag="JQG95_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY5889798.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8793..>10648
                     /gene="rsxC"
                     /locus_tag="JQG95_003348"
     CDS             8793..>10648
                     /gene="rsxC"
                     /locus_tag="JQG95_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY5889799.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPR"
BASE COUNT         2455 a         2757 c         2717 g         2719 t
ORIGIN      
        1 aagtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
       61 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata
      121 ccaagttccc gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt
      181 gtcgcatcgg taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt
      241 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
      301 ttatagcaaa ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
      361 gcggcttgct tctggctgga agtgcactcc aacacccaat cgctgtgaaa cggcaggcca
      421 aattttagta gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt
      481 tgccctccca gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
      541 tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg
      601 gaagcgaaaa tcaccgggat agctttttct tctgccatct gccgcaggtc atcgctgctt
      661 cctgcagccc cggcaattac cacaccatcg acaccctgat tcagtaacag tgaaaaccgc
      721 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgtgct
      781 tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg
      841 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg tcgattgcgc
      901 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
      961 cctttgccat taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     1021 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     1081 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     1141 tttatgactc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     1201 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     1261 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     1321 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc
     1381 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     1441 ttgcttttag tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     1501 aaaataaagg cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     1561 taaacttctg gttgaccaat aaaggcattc tgccaaccac agatgccgcc gttctgaaag
     1621 ccaataacat ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     1681 tgatcgccgg tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg
     1741 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg
     1801 gccttgtcgg cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct
     1861 tgggccatat gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     1921 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     1981 caggcggcac gcaggaggtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     2041 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgtcacgcgt ttcctttcgc
     2101 aaggtaaaat gcctgcgttt ctcggcggtc taccaggtgc agcgttagct atgtatcact
     2161 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct
     2221 gcgtcgttgg cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     2281 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     2341 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     2401 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     2461 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     2521 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     2581 ttcctgcaat cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     2641 ttacccgtct gcgtttgtct gtggaagata tgtcgcttgt taatgtgcag gcactgaagg
     2701 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     2761 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     2821 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt
     2881 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     2941 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     3001 tatttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     3061 ccacccagca ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct
     3121 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     3181 cgcccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     3241 ttgctttaga gaagcaggct gacggctggt tttgcgatat gggcaagctg gaagccgttt
     3301 tggcgaaacc agaatgtaaa attatgctcc tgtgtaaccc acagaatcct accgggaaag
     3361 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat gatgtgcggg
     3421 ttatttccga tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga
     3481 gtaatgtggc gcggggagac tgggcgttgc tgacatcggg atcgaaaagt ttcaatattc
     3541 ccgcgctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     3601 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata
     3661 tcgccgccta tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     3721 acctgacgta tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc
     3781 cgcaatccac ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt
     3841 tgcaaaaagc gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg
     3901 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag
     3961 gtgtggctgg attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa
     4021 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     4081 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     4141 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     4201 atctcgcttc ctgcacaatc ccttgaaaca ctgattcccc acgttcaggt cattgccaac
     4261 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     4321 cttgatgcct gccgccgcgt ggcatttgaa aacattgaag atgcagcccg taacggcctg
     4381 cgctatgtcg agctgcgttt ttcaccaggc tacatggcaa tggcacataa gctgcctgta
     4441 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     4501 caggcgaagc ttatcggcat tatgagccga accttcggcg aagccgcctg tcagcaagag
     4561 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc tggtgatgaa
     4621 cttggattcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     4681 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     4741 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
     4801 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
     4861 agcaccgtag cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt
     4921 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
     4981 gttgccgcgc cagctgctgg gttatcccgt gagcaaatcc gccaggcaca gattaatggt
     5041 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
     5101 aagtaacaaa aatggatggt gcaaattgca ccatccattt ttcacgcaag gcacaacgtc
     5161 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctgg
     5221 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatcgc cgcatagtaa
     5281 gccggataat tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg
     5341 cgggtaagta cgccatcacg catatcgtag ccccagtctt cctgcggcag acgctcgcca
     5401 tttttcagac gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
     5461 tgcacgatat accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc
     5521 cgctgtggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
     5581 tgtgccaaat ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga
     5641 agatgcggtg ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga
     5701 tcgcgcacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc
     5761 acgccttccg cgagcaaacc ttttagcgtc aagaaatcgc tatcccaacg acggttatgg
     5821 aatacagaca gcacacgccc caggcttttt gccagcgcat ccagctctcg cgcttgtgac
     5881 agtgtcacgg taaagggttt atcaacgacc acatatttac ccgcctcaag cgccgctttg
     5941 gctaacggga aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg
     6001 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
     6061 gtttcatcac tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
     6121 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
     6181 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca
     6241 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
     6301 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
     6361 attattgcta agtaacattt accccctgaa gttaatggat caatcaagag agatgtgggc
     6421 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
     6481 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca
     6541 acacaagtaa gctctgcact tgtggagcga catgctgccc gtccgggtgc atgttttcac
     6601 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg
     6661 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
     6721 gcattcttat tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa
     6781 taatacctac ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac
     6841 ttattaaaat ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt
     6901 aattacaccc tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt
     6961 cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc
     7021 tggcactatg tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc
     7081 taaaattaga tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc
     7141 acaacgtatt ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttgag
     7201 tacgacgctg gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac
     7261 gctcggcgga caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat
     7321 cgccatgtgg tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt
     7381 tcctaaacac tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc
     7441 tttttcaccc gtggaagttg gcatagccgt tcgccagttg ctgtttactt tgttggcaac
     7501 cgcattgatc gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc
     7561 gtaataacct tacagttaac ctgttgccgc ctgctctgga ttaacggata ataggcggct
     7621 tttttatttc aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg
     7681 tcaataactt tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa
     7741 aactggaaac cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctgacgtcta
     7801 tttgcgcctg gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca
     7861 ccctggcatt tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca
     7921 aaaccagccc ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact
     7981 gtgcagtgct cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg
     8041 cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca
     8101 tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt
     8161 taattaccgc aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt
     8221 aatgaatgct atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc
     8281 cattctgggt tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat
     8341 tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta
     8401 cgcggaagcc atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc
     8461 tgtgatgcta aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc
     8521 gcaagagcta acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg
     8581 cactaaatgt attcaggctt gtccggtaga cgccatcgtt ggcgctaccc gtgtcatgca
     8641 tacggtaatg agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca
     8701 ctgcatctcg ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac
     8761 cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa
     8821 aaaataaaat ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagacca
     8881 acggtacccc cctgcgccag gtactactgg cgcagcattt tgttattcca ctgaaacagc
     8941 atattggcgc tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc
     9001 cgcttacccg tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta
     9061 cggctattgc gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta
     9121 ttgatgccga tggtgaagac tgctggatcc cgcgcgacgg ctgggtcgat tatcgctctc
     9181 gcagtcgcga agagttaatc gagcgcatac atcagtttgg tgttgccggg ctgggcggtg
     9241 ccggattccc gacaggcgtt aaattgcagg gtggtggaga taagattgaa acgttgatta
     9301 tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg
     9361 cggctcaggt cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc
     9421 ttatcggcat tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg
     9481 actctaacga tatttctctg cgggtgattc caaccaaata tccttctggc ggtgctaaac
     9541 aattaaccta cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg
     9601 gcgtattaat gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg
     9661 agccgattac cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg
     9721 tctgggcacg gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct
     9781 ctgccgatca aatggtgatt atgggtggcc cgctaatggg ctttaccttg ccatggctgg
     9841 atgtcccggt cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg
     9901 aaccacagga agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgtgg
     9961 atcttttgcc gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca
    10021 cgcataacat tgccgactgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata
    10081 ttcccttggt gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag
    10141 aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg
    10201 aaaaagcgac tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata
    10261 aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc
    10321 ctattgtgat taaagcgggc gaacgcccgg ataacagtgc aattattgca gaacgggaag
    10381 cccgtaaagc gcaagccaga gcgaaacagg cagaactgga gcaaactaac gacgcagcaa
    10441 ccgttgctga accacgtaaa actgccgttg aagcagctat cgcccgctcc aaagcgcgca
    10501 agctggaaca gcaacaggct aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg
    10561 ccgtcgaagc cgctattgcc cgcgccaaag cacgcaagct ggaacagcaa caggccaatg
    10621 cggagccaga agaacaggtt gatccgcg
//
LOCUS       DADLCQ010000132        10564 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000132 DADLCQ010000000
VERSION     DADLCQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.133"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            50..1540
                     /locus_tag="JQG95_003349"
     CDS             50..1540
                     /locus_tag="JQG95_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY5889800.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1592..2749
                     /locus_tag="JQG95_003350"
     CDS             1592..2749
                     /locus_tag="JQG95_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY5889801.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2715..2837)
                     /locus_tag="JQG95_003351"
     CDS             complement(2715..2837)
                     /locus_tag="JQG95_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889802.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3040..4725
                     /locus_tag="JQG95_003352"
                     /pseudo
     CDS             3040..4725
                     /locus_tag="JQG95_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4763..7123
                     /locus_tag="JQG95_003353"
     CDS             4763..7123
                     /locus_tag="JQG95_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY5889803.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7168..9963
                     /locus_tag="JQG95_003354"
     CDS             7168..9963
                     /locus_tag="JQG95_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY5889804.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNELGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYISYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            10324..>10564
                     /locus_tag="JQG95_003355"
     CDS             10324..>10564
                     /locus_tag="JQG95_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5889805.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         3126 a         2325 c         2284 g         2829 t
ORIGIN      
        1 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
       61 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      121 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      181 aacgccgacg ctcctttcat taatggatga aggcgtacgt tttactaacg gctatgtggc
      241 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      301 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      361 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      421 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      481 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      541 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      601 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      661 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctacc
      721 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
      781 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
      841 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
      901 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
      961 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1021 ttatgacaag ctgatttccg caatggattt ctacccaaca gctcttgatg cagccgatat
     1081 cagcattcca aaagacctta agctggatgg tgttcccttg ctgccctggt tgcaagataa
     1141 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1201 tgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
     1261 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1321 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1381 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1441 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaggtaa atcaggagaa
     1501 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1561 tttttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1621 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1681 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgaagcgtc
     1741 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     1801 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     1861 attacaaacg aatggcattt tattgaataa tgaatggtgt gcctttctca aagaacatga
     1921 atttctggtt ggtatctcga tcgatggccc ccaggagtta catgaccgtt acagacgcag
     1981 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2041 tcaagtagag tttaatacgt taaccgtcat taataacgtt aatgtccatt atcctcttga
     2101 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctaga
     2161 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2221 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2281 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     2341 ggggaatggg catacaagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2401 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2461 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2521 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2581 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaat
     2641 ccttttttca accatggtac cttatatgaa cgccatggtt gagttagcta agaacagagt
     2701 accgctttac cacattatgg atgttgcaaa acaaatggag aataattaat aacgctctct
     2761 tttaactcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     2821 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     2881 cagggcatta gcaataccct tgattatcaa tagcataacc ataaccactg aaaattaaaa
     2941 atttctatat atttcatatt gttaaatgat gcttaaataa atttcatcag taaatcattg
     3001 ccatagataa tcattggatg gcacaatcaa ttttcgctaa tgataaccat tcccattacg
     3061 ttttgtatgt tgatagcaaa gtatctctgt ctgctgaagc ccttttggct gcgtaagaat
     3121 aataaaactt cagtgttgtt aatcattatt atcctcgcaa tgatccttgg cgtggttaaa
     3181 attcaggtct ggttaaacga ctggaataat gattttttca atgcgctaag ccagaaagaa
     3241 accgataagc tttggcagct cgttctttgg ttccctgccc tgctggggat ctttgtatta
     3301 atatccgtaa acaaaacatg gttaattaaa ctactgacta ttcgctggcg cgaatggtta
     3361 acagattact accttaatcg ctggtttgca gataaaaact actacttcac gcagatctac
     3421 ggcgagcata aaaatacaga taaccctgac caacgtatcg ctgaagacat tctcttactg
     3481 atcaacaaaa cgctcagtct ttcatttggc ttcatccagt cacttagcat gctgatcacc
     3541 tttaccgtta ttctttggca aagcgcgggt acgctctcat tcactgttgg tggaacagaa
     3601 tggaatatcc aggggtatat ggtctatacc gtcgtgctca ttgtgatcgg tggaacttta
     3661 tttacccata aggtgggtaa acgtattcgt ccgcttaatg tggaaaaaca acgtagcgaa
     3721 gcgacatttc gaactaatct tgtgcagcat aataagcagg cagaactgat tgcgctaagc
     3781 aatgctgagt ctctacaacg ccaggagttg cgcgacaatt ttcataccat taaagagaac
     3841 tggcatcgtt taatgaatcg tcagcggtgg cttgattact ggcaaaatat ttattcgcgc
     3901 tcgcttagcg ttcttcccta ctttctgtta ttgccgcagt ttattagcgg gcagatcaat
     3961 ctgggcggac tgatgaaatc gcgccaggca tttatgctag tatcgaacaa tttaagctgg
     4021 tttatttata aatatgacga acttgctgaa ctggctgcgg ttatcgatcg cttgtatgag
     4081 ttccatcaac tcactgaata gcgccctacg aataagccta aaaattgcca acatgcggta
     4141 caagtggcta atgcgagtat tcgtacgcct gataataaga tcatattaga gaacttgaac
     4201 tttcatgttt cgccaggcaa atggctatta ctgaaaggct actctggtgc gggaaaaacc
     4261 acactgctta aaacattatc ccactgctgg ccgtggttta aaggtgatat ttcttctcct
     4321 gctgacagtt ggtatgtgtc acaaacaccg ttaatcaaaa gcggcttact gaaagagatt
     4381 atttgtaaag cacttcccct gcccgtagac gataaatcgt tgagcgaagt actgcatcag
     4441 gttggtctgg gaaaattggc tgcgcgtatt catgaccacg atcgctgggg agatattctt
     4501 tccagcggcg aaaaacaacg tatcgccctg gcacgattaa ttttacgacg tccgaaatgg
     4561 atatttcttg acgaaactac ctctcatctt gaggaacaag aggctatacg cttactgcgt
     4621 ttagtgcgtg aaaaactacc cacaagcggc gtcattatgg ttacacatca acccggtgtc
     4681 tggaacctgg ctgatgatat ttgtgacatt agcacggttt tataacaaca ataaaagccc
     4741 tgctgtacgg agatataaat aaatgaagcg agttcttatt cctggcgtca ttttatgtgg
     4801 cgctgatgtg gcgcaggccg tcgatgacaa aaacatgtac atgtattttt ttgaagagat
     4861 gacggtctat gctcctgtcc ctgtacccgt aaacggcaac acgcattaca ccagtgaaag
     4921 catcgagcgt ttaccgaccg ggaatggcaa tatcagcgat ctgctgagaa ccaaccctgc
     4981 ggtacgcatg gattcaacgc aaagtacctc gttgaaccag ggagatattc gccctgagaa
     5041 aatctctatt cacggtgcgt cgccctacca gaatgcctat ttgattgacg gtattagtgc
     5101 aactaataac ctgaacccag cgaatgagtc cgatgccagt agtgcaacca atattagcgg
     5161 gatgtcacag gggtattatc ttgatgtcag cttactggac aatgtgacgc tttatgacag
     5221 ttttgtgccg gttgaatttg gtcgcttcaa tggcggggta attgatgcaa agatcaaacg
     5281 cttcaacgct gatgatagca aggtgaaatt gggttatcgc actacgcgtt tggactggtt
     5341 aacatcgcat atcgatgaga ataacaagag cgcatttaat caaggttctt caggaagtac
     5401 ctatttctct ccagatttta aaaagaactt ttataccttg tcgtttaatc aggagctggc
     5461 tgataacttt ggcgttaccg ccggtttatc gcgccgccag tctgatatca cccgcgcgga
     5521 ttatgtttcg aatgacggca ttgtcgccgg tcgggcacag tataaaaacg ttatcgatac
     5581 tgcattgagc aaatttacct ggtttgccag cgaccgcttt acccacgatt taaccttaaa
     5641 atataccggc tccagccgtg attataatac cagcaccttc ccgcagtctg atcgcgaaat
     5701 gggtaataaa tcctatggtc tggcatggga tatggatacg caactcgcat gggccaaact
     5761 acgtaccacc gttggttggg atcatattag tgattatacc cgtcacgatc atgacatctg
     5821 gtacaccgaa ctttcatgta catatggtga tattacaggg cgttgtaccc gtggcggatt
     5881 aggacacatt tcccaggctg tagataatta caccttcaaa acacgcctgg actggcaaaa
     5941 attcgccgtg ggtgatgttt cgcatcaacc ctacttcggc gcggaataca tctattccga
     6001 tgcatggact gaacgccata accagtctga atcctatgtg attaatgctg caggaaagaa
     6061 aactaaccat accatttacc ataaaggtaa aggcagcctg ggaattgaca actacacgct
     6121 gtatatggcg gatcacatta gctggcggaa tgtgtcgtta atgcccggtg tgcgttatga
     6181 ctatgacaac tatctgtcaa accacaatat ctccccgcgc tttatgacgg aatgggatat
     6241 ttttgctgat caaacctcaa tgattaccgc cggttataac cgttactatg gcgggaatat
     6301 tcttgatatg ggattacgtg atatccgcaa tagctggacg gaatcggtat caggtaataa
     6361 aaccctgacg cgttatcaga atttgaaaac gccttataac gatgaactgg caatgggatt
     6421 gcagcaaaaa atcgataaga acgttattgc acgcgcaagt gaagcgcatg atcaaatcag
     6481 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
     6541 caaaaccaaa acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg
     6601 ccaggtagat attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc
     6661 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
     6721 tctggtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
     6781 aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca
     6841 agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata
     6901 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
     6961 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
     7021 caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc
     7081 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
     7141 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
     7201 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
     7261 gatgaaaagt taattaccgg gcaactggac aatgacttgc gatatatgat ttatccgcat
     7321 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
     7381 gaagacaatg agctcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
     7441 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
     7501 gatgttaatg cctatatcag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
     7561 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
     7621 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
     7681 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
     7741 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac gccggcacaa
     7801 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
     7861 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
     7921 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
     7981 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
     8041 gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag
     8101 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
     8161 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
     8221 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
     8281 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
     8341 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
     8401 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
     8461 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
     8521 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
     8581 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
     8641 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
     8701 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag
     8761 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat
     8821 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
     8881 gttagcggaa gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa
     8941 aactcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
     9001 cggactaata accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg
     9061 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
     9121 gaccagcgtc cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac
     9181 cgcacgaaat tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct
     9241 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
     9301 gcagaagaca aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat
     9361 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
     9421 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
     9481 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
     9541 ctacgcgtta atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg
     9601 gttgatcctc aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
     9661 catgatgaac agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc
     9721 agtgagcaag aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag
     9781 cgtagcgttc aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct
     9841 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
     9901 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
     9961 taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat
    10021 gtaataattt tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg
    10081 cctgattttt acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa
    10141 cacctttcca ccacccaaga attctctatt acattgtttt taatgaaaaa atatcattcc
    10201 aacaatatct cacgataaat aacattagga ttttgttatt taaacacgag tcctttgcac
    10261 ttgcttactt tatcgataaa tcctactttt ttaatgcgat ccaatcattt taaggagttt
    10321 aaaatggata agaagcaagt aacggattta aggtcggaac tactcgattc acgttttggt
    10381 gcgaagtcta tttccactat cgcagaatca aaacgttttc cgctgcacga aatgcgcgac
    10441 gatgtcgcat ttcagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    10501 ctggccactt tctgccagac ctgggacgac gaaaacgtcc acaagttgat ggatttatcc
    10561 atta
//
LOCUS       DADLCQ010000133        10529 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000133 DADLCQ010000000
VERSION     DADLCQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.134"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..95
                     /locus_tag="JQG95_003356"
     CDS             <1..95
                     /locus_tag="JQG95_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5889806.1"
                     /translation="KGVKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            154..540
                     /locus_tag="JQG95_003357"
     CDS             154..540
                     /locus_tag="JQG95_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY5889807.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            549..878
                     /locus_tag="JQG95_003358"
     CDS             549..878
                     /locus_tag="JQG95_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY5889808.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            850..3588
                     /locus_tag="JQG95_003359"
     CDS             850..3588
                     /locus_tag="JQG95_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY5889809.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARAAQLGVSSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGN
                     FGALRGSGITLANRAGWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFN
                     RQLALTGHYAGVTVGQLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKA
                     AAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSS
                     DAARIAMSALAEETGKRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQ
                     IDALQEKIQRAKKYPWTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQK
                     RRNAENAALNRMNETEAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTR
                     ATRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDG
                     KKLTADEKSVLARKNELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERAL
                     REQHNLDIATAGMGDKQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEE
                     VLKGSLKRQLNENKRYWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFS
                     SAGNALATFCTTGKLNFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGV
                     YQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSG
                     MVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            3588..3917
                     /locus_tag="JQG95_003360"
     CDS             3588..3917
                     /locus_tag="JQG95_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5889810.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            3917..4615
                     /locus_tag="JQG95_003361"
     CDS             3917..4615
                     /locus_tag="JQG95_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY5889811.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            4620..5363
                     /locus_tag="JQG95_003362"
     CDS             4620..5363
                     /locus_tag="JQG95_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5889812.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYMLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            5300..5902
                     /locus_tag="JQG95_003363"
     CDS             5300..5902
                     /locus_tag="JQG95_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY5889813.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            5963..9443
                     /locus_tag="JQG95_003364"
                     /pseudo
     CDS             5963..9443
                     /locus_tag="JQG95_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            9511..10110
                     /locus_tag="JQG95_003365"
     CDS             9511..10110
                     /locus_tag="JQG95_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY5889814.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            10174..>10529
                     /locus_tag="JQG95_003366"
     CDS             10174..>10529
                     /locus_tag="JQG95_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5889815.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGA"
BASE COUNT         2451 a         2467 c         3401 g         2210 t
ORIGIN      
        1 tgaaaggcgt gaagcagggc agtgtcagca tcgtgggcat gacctctgac ggagagtttg
       61 tggcagtggc tgcggtgacc gtcagcgcac cataacagga cgatactcat cattgccccg
      121 gttatccggg gctttttgca tccggagaac atgatgtttc tgaaacagga tacgtttaat
      181 tatgaaaaac agtccgtggt gctcagtgag ctgtccgggc tgcagagaat tgaatatctg
      241 acgtttgttc agcagcgaac ggcaaagttt gatgcacagg agggagaact gccggaggct
      301 gaacgacaga ttgcttttct gcgtatggga atggatatca atgcctggct ggtttcccgc
      361 tcactgtgga atgctgagca gtctcaggat gttgagacgc tttgcgcatc cattatgaca
      421 acatggtcgt atgatgcgct gggcgcgggg gcggagaggg ttctgtcgct gagcggtatg
      481 gggaccattg agaatgccgg ggatgatgat catgaggcgc tgacgccgga aaagtcctga
      541 cgcgggaaat gcagtttgtc atgcggcttg cccgggagtt ccggcgggca gactggcggc
      601 ggatgctgtc ggatatgtcg gccactgagc ttggtgagtg gggcgattat ttccggatgc
      661 agagcttcag tgatgtgtgg atggatgcgc agtttgcctc gctgaaggca ttgatcgtga
      721 gaatggtgtc cggcagcagc gatgctgcgg tggctgattt cagcctttta ccggaagaga
      781 acgggatacc ggagcgaacg gacgaagaac tgatgcatct tggggaaggt atttccggag
      841 gtgtgcgtta tggaccagat agccaacctg gtcattgatt tggggattga tgcggcagag
      901 tttaaaaatg aaattccccg tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa
      961 cggtcttctg cccgtatgca gcgttttatg gagcgtcaga ctcaggcggc ccggcagaca
     1021 atgcaggcgg cttcttcggc tgcaacagca gcatcagccc atgcgcagac ggtggagaag
     1081 aatgcacggg ctcatgaacg catggcccgt gaggtggaac aaacccgtct gcgcgtggat
     1141 gccctgaatc agaaaatgcg cgaggaacag gcgcaggcca gggcggctca gctgggggta
     1201 agcagtgctg cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg
     1261 ctggggctga aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg
     1321 cgcggcaatt ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg
     1381 acaggcgcac tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc
     1441 gcggcggtcc tgggtctggg taaagcctgg catgacggcc ggaaggaggg cgagaaattt
     1501 aaccgtcagc tggcgctgac gggacattat gccggtgtca ctgtcgggca gttgtggaaa
     1561 ctcagccggg ccatatccgg gaatggtatc acgcaacatg cggcagccgg tgcgctggca
     1621 caggtggtgg gcagtggggc atttcatgga aacgatattg gtatggtggc gaaagctgcc
     1681 gcacagatgg agcgatcggt tggccagtcg gtcagcgata ccataaatca gtttaagcgg
     1741 ctgaaggatg atcctgtaaa tgccgcgaag gcgctggaca atgcgctgca ttttttgaat
     1801 gccactcagc ttgagcagat acgcgtcctt ggggagcagg ggcggtccag tgatgcggcc
     1861 cggatcgcca tgtctgcgct ggcagaggaa accggtaaac gcacatctga tattgataat
     1921 aatctcaatg cgctgggtag tacgctgcaa accttgtctg actggtggaa gcagttctgg
     1981 gatgcggcca tgaatattgg tcgtgaagac tcgctggatg cgcagattga tgcgttacag
     2041 gaaaaaattc agcgcgcgaa aaaatatccg tggacaaacg cctccacaca ggtggagtac
     2101 gatcagcagc gtcttaacga tcttcaggag aaaaaacgcc ggaaggattt gcaggatgca
     2161 aaagcgcagg cagaacggaa ttaccaggag caacagaaac gccggaatgc tgaaaatgcc
     2221 gcgctgaacc ggatgaatga aacggaagct gcacgacatc agcgggaaat tgcgcgtatt
     2281 aatgccatgc agtacgccga ccaggctgtc agggatgcgg cgatacagcg tgaaaatgaa
     2341 cgttacgaaa aagccattaa gaaaaataca cgggcaaccc gtaatgatga ggccacccgg
     2401 ttattgctgc agtacagtca gcagcaggca caggtggaag ggcagattgc tgctgccaga
     2461 cagtcagcag gcattgccac tgaaaggatg actgaagcgc ataaacagct tctggccctg
     2521 cagcagcgca tcagcgacct ggacgggaaa aagctgacgg cagatgaaaa gagtgtgctg
     2581 gcccgtaaga atgagctgat tcaggcgctg acgctgctgg atgtgaaaca gcaggagctg
     2641 cagaaacaga cagcgcttaa cgacctgaga aaaaaaacgg ttcagctgac cagccagctg
     2701 gcagacaaag aacgtgcact gcgtgagcag cacaatctgg atattgccac tgcaggtatg
     2761 ggggataagc agcggcagcg ctaccaggca cagttgcgca tccggcagga ataccggcaa
     2821 cagttgcaac agcttgagaa tgacagtcgc cagaaaggca cttacgggac ggaggactac
     2881 cggagggctg aggaggtgct gaaggggagc ctgaagcgac aactgaatga aaacaaacgc
     2941 tactggcagg aactggaagt ggcgcagggc gactggaaaa acggtgccat gcgggcgttt
     3001 cagaatttta cggcggatgc ggataatgcg gcgggaacgg cagaacagat gtttacagtg
     3061 gcattcagca gtgccggtaa tgcactggcg acattctgta ccaccggtaa gctgaatttt
     3121 aaatccttca cctcttccct gttgtcagat atggccagaa ttatggcaca gatggccatg
     3181 atgcaggcgg taaagggcgt cggttcttta ttcggcttca cgactaatgc tgatggcggt
     3241 gtttaccagt ctgctgattt gagtcgctac agtggcacgg tggttaaccg tccgacgttt
     3301 tttgcttttg caaaaggcgc gggtgtgatg ggggaagcgg gacctgaagc cattctgcca
     3361 ctgcgtcgcg gtgctgatgg taagctgggg gttgtggcgg atactggtgg ttcaggcatg
     3421 gtgatgtttg ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata
     3481 ggtccggctg ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg
     3541 caacagcagg cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc
     3601 actggaaagt gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc
     3661 tgggcgatgg ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca
     3721 gcgtgacgat acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc
     3781 acggtggcgt ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggtgg
     3841 tttgtcgtaa atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg
     3901 aacaggtggt gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc
     3961 ggagcagtca gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg
     4021 ttattttttc tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca
     4081 gtatcaggca taccccattc aggggtcggg gtttgaactg aacggcaggg gctgtgctgc
     4141 ccgtccgaca ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct
     4201 gcagagtctg gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc
     4261 ggtgaacttc gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg
     4321 gcgcatcgag cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc
     4381 aacggagacg gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg
     4441 gacttaccgt tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa
     4501 accaacaacg gatatccgga aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg
     4561 caacaatacc ggtaatttcg gcggtttcct ttccatcaat aaactttctc agtaaatcca
     4621 tgacacagac agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt
     4681 gtggcttcgt ggtgagaacg ccggagggag acaggtatct tcccagcgag aatatctccg
     4741 gtgagccgga ggaacggttc cggatggctc cggaggactg gctgcgggca caaatgcagg
     4801 gtgagattgt ggcactggtc cacagtcatc ccggtggtct gccctggctg agtgaggctg
     4861 accggcggct gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg ggggcgattc
     4921 acaagttccg ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact
     4981 gttacatgct gttccgggat gcttatcatc tggcggggat tgagatgccg gattttcatc
     5041 gcggggatga ctggtggcgt cacggtcaga atctctatct ggataatctg gaggccacag
     5101 ggctgtatca ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg
     5161 gttcatcggt gccgaatcat gccgccattt actgcggcga tggtgagctg ctgcaccata
     5221 ttcctgaaca actgagtaaa cgagagaggt ataccgacaa atggcagcga cgcacacact
     5281 ccctctggcg tcaccgggaa tggcacgcat ctgcctttac ggggatttgc aacgatttgg
     5341 ccgccgcatc gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca
     5401 gctcccggcg tttcgtcaga aactgagtga cggctggtat caggtgcgca ttgccgggcg
     5461 tgatgcaggt gaaaccgaat tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt
     5521 gatccacata gtaccgcgtc tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct
     5581 gggggcagct gttatggcgg ttgctatatg gatgccgggg gtaggaatta tggcgagtaa
     5641 tctgctgttt tctctcggtg ccagtatgac gcttggcggt gttgcacaga tgctggcacc
     5701 gaaagccaga actccccgta cacagacaac ggataacggc aaacagaaca cctgtttctc
     5761 ctcactggat aacatggttg cccagggcaa tgttctgcct gttctgtacg gtgaaatgcg
     5821 cgtggggtcg cgggtggtat ctcaggagat cagcacggca gacgaagggg atggtggtca
     5881 ggttgtggtg attggtcgct gatgcaaaat gtttcatgtg aaaccgcctg cgggcggttt
     5941 tgtcgtttat ggagcgtgag gaatgggtaa aggcagcagt aaggggcata ccccgcgcga
     6001 agcgaaggac aacctgaaat ccacgcagtt gctgagtgtg attgatgcca tcagcgaagg
     6061 gccggttgac ggtccggtgg atggattaaa aagcgtgctg ctgaacggta cgccggtcct
     6121 ggatagcgag gggaagacaa acttttccgg tgttacggtg gtgttccgcg ccggcgagca
     6181 ggagcagaca ccgccggagg ggtttgaatc ttccggctca gagactgtgc tgggtacgga
     6241 agtgaaatac gacacgccga tcacccggac catcacgtcg gcaaacattg atcgtctgcg
     6301 cctgaccttc ggtgtgcagg cactggtgga aaccacctca aagggggacc ggaatccgtc
     6361 ggaagtccgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
     6421 caccattaag ggcaaaacca cttcgcagta tctggcctcg gtggtggtgg gtaacctgcc
     6481 gccgcgcccg tttaatatcc ggatgcgcag gatgacaccg gacagcacca cagaccagct
     6541 gcagaacaaa acgctctggt cgtcgtacac cgaaatcatc gatgtgaaac agtgctaccc
     6601 gaacacggcg ctggtcggcg tgcaggtgga ttcagagcag ttcggtaacc agcaggtgag
     6661 tcgcaattat catcttcgcg ggcgcattct gcaggtgccg tcgaactata acccgcagac
     6721 gcggcaatac agcggtatct gggacggaac gcttaagcca gcatacagca acaacatggc
     6781 ctggtgtctg tgggacatgc tcactcatcc gcgctacggc atggggaaac gtcttggtgc
     6841 ggcagatgtg gacaaatggg cgctgtatgt catcggccag tactgcgacc agtcagtgcc
     6901 ggacggattt ggcggcacgg agccgcgcat cacctgtaac gcttacctga ccacacagcg
     6961 taaggcgtgg gatgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa
     7021 cgggcagacg ctgacgttcg tgcaggaccg accgtcggat aaagtgtgga cctataaccg
     7081 cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagcttca gcgccctgaa
     7141 ggaccgccat aatgtcgttg aggtgaactg gattgatccg gacaacggtc atgagacggc
     7201 gacagagctt gtggaggaca cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat
     7261 ggatgccttt ggctgtacca gccggggaca ggcgcaccgc gccgggctgt ggctgattaa
     7321 aacggaactg ctggaaacgc agaccgtgga cttcagcgtc ggcgcagaag ggcttcgcca
     7381 tgtaccgggt gatgtcattg aaatctgcga tgatgattat gccggtatca gcaccggtgg
     7441 tcgcgtactg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct
     7501 gccgtcctcc ggtaccacgc tgataagcct ggttgacgga aatggcaatc cggtcagcgt
     7561 ggaggttcag tccgtcaccg acggcgtgaa ggtgaaagtg agccgggttc ctgacggcgt
     7621 tgcagaatac agcgtgtggg ggctgaagct gccgacgctg cgccagcgcc tgttccgctg
     7681 cgtgagtatc cgtgagaacg acgacggcac gtatgccatc accgccgtgc agcatgtgcc
     7741 ggaaaaagag gccatcgtgg ataacggggc acactttgac ggtgaccggc gcggcacggt
     7801 gaatggtgtc acgccgccag cggtgcagca tctgaccgcc gaagtcaccg cagacagcgg
     7861 ggaatatcag gtgctggcgc gatgggacac gccgaaggtg gtgaagggtg tgagcttcct
     7921 gcttcgcctg accgtggcag cggacgacgg cagtgagcgg ctggtcagca cagccaggac
     7981 gacggaaacc acataccgct tcaggcagct ggcgctgggg aattacagtc tgacagtccg
     8041 ggcggtaaat gcccgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgcggc
     8101 accggcagcg cctgtcacta ttgaactgat accggggtat tttcagataa cggtggtccc
     8161 gaaactggct gtatatgacc cgacggtgca gtttgagttc tggttttcgg aaaagcggat
     8221 tgcggatatc aggcaggttg aaaccagcgc ccgctatctt ggcacggcgc tgtactggat
     8281 agccgccagt atcaatatca ggccgggcca tgattattat ttttacgttc gcagtgtgaa
     8341 caccgttggc aaatcggcat ttgtggaggc cgtcggtcgg gcgagcgatg atgcggaagg
     8401 ttatctgagt ttttataaag ggttgatcaa taaaacgcat ctcggcaagg agttgtggac
     8461 gcagattgat aacggtcagc ttgcgccgga cctgactgaa atcaggacgt ccattacgaa
     8521 tgtcagcaat gaaatcacgc aaaccgtcaa taaaaaactg gaaaatcaga gtgctgcaat
     8581 ccagcagata cagaaagttc aggttgatac aaataataac ctgaacagca tgtgggctgt
     8641 gaagctgcaa cagatgaagg acggacgcct ttatattgcg tggtatcggt gccggtattg
     8701 agaatacgcc agcaggaatg cagagtcagg tgctgctggc ggcagacagg attgcgatga
     8761 ttaatcctgc gaatggcaac acaaagccga tgtttgttgg tcagggcgat cagatattca
     8821 tgaacgacgt gttcctgaaa cgcctgacgg ctccgaccat taccagcggc ggtaatcctc
     8881 cggcattttc cctgacacct ggcggacggc tgacggcgaa aaatgccgat atcagcggta
     8941 acgtgaacgc gaactccggg acgctcaaca acgtcacgat taataagaac tgtcgggttc
     9001 tgggaaaatt gtccgcgaac cagattgaag gcgatctcgt taaaacagtg ggcaaacctt
     9061 tcccacggga ctcccgggca ccggagaggt ggccatcagg gaccattacc gtcagggttt
     9121 atgacgatca gccgtttgat cggcaaattg ttattcccgc ggtggcgttt cgcggtgcta
     9181 aacatgagcg gaagaataac aatatttatt cgtcatgccg cctgatagtg aagaaaaacg
     9241 gtgctgaaat ttataaccga acgaccctgg ataatacgct gatatatacg ggtgttattg
     9301 atatgcctgc cggtcacggt cacatgacgc tggagttttc tgtatcggca tggctggtaa
     9361 atggctggta tcccacagca agtatcagcg atttgctggt tgttgtgatg aagaaatcca
     9421 cagcaggtat cagtatcagc tgaattttat aacccagata cgggcaccag aaatggtgcc
     9481 ttttttattg cagaaaagcg agaggtaatt atgcgtaaac tttatgctgc cattttgtcc
     9541 gcagccatct gtctggctgt atccggtgcg cctgcatggg cgtctgaaca tcagtccacg
     9601 ctgagcgcgg ggtatcttca tgcctcgacg aacgctcccg gcagtgatga tctgaacggg
     9661 attaacgtga aataccgtta tgagtttacg gacacgctgg ggctggtgac gtcattcagc
     9721 tatgccaacg ctgaagatga gcaaaaaacg cattacagtg atacccgctg gcatgaggat
     9781 tccgtgcgta accgctggtt cagcgtgatg gcggggctgt ctgtgcgcgt gaatgaatgg
     9841 ttcagcgcgt atgcgatggc gggcgtggct tacagccgtg tgtcgacctt ctccggggat
     9901 tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg gagtgatgac
     9961 aaccgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa
    10021 tccgtggcca ttgaccttgc ttatgaaggt tccggcagtg gcgactggcg cactgacggt
    10081 ttcatcgtgg gtgtcggtta taagttctga ttagccaggt aacacagtgt tatgacagcc
    10141 cgctggttca ggcgggcttt ttgtggggtg aatatggcag taaagatttc aggtgtactg
    10201 aaagatgggg cgggtaaacc tgtcgtaaat tgtgcgattg aactgcgggc cagaagaacc
    10261 agtccgaccg ttgtggcaca cgttgttgcc acttgcgtga cggacaataa cggtgcttat
    10321 gtgattgagg ctgagccggg gtattacgag gttgcgcttc actgtaacgg ctggcagcca
    10381 acccgtgtcg gggatattga tgtggcaccg actgatgcac cggggacact gaacgcgttt
    10441 ctgaatgcac caaaggatgg tgatttacgt ccggaggtga tgaagcgctt tgaggaaatg
    10501 gtggcgcagg cgcagcagag tgccggggc
//
LOCUS       DADLCQ010000134        10352 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000134 DADLCQ010000000
VERSION     DADLCQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.135"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..431
                     /locus_tag="JQG95_003367"
     CDS             <1..431
                     /locus_tag="JQG95_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="participates in controlling several genes involved
                     in isoleucine and valine biosynthesis; activates the
                     transcription of the ilvC gene in the presence of
                     acetolactate or acetohydroxybutyrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY5889816.1"
                     /translation="AVAFSMLENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQ
                     GPVRRRIELWFRRNKISNPMIYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRN
                     RVMILERSDEKTPFELGVCAQKKRLHEPLIEAFWKILPNH"
     gene            complement(428..1972)
                     /gene="ilvA"
                     /locus_tag="JQG95_003368"
     CDS             complement(428..1972)
                     /gene="ilvA"
                     /locus_tag="JQG95_003368"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY5889817.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(1975..3825)
                     /gene="ilvD"
                     /locus_tag="JQG95_003369"
     CDS             complement(1975..3825)
                     /gene="ilvD"
                     /locus_tag="JQG95_003369"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY5889818.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(3890..4819)
                     /gene="ilvE"
                     /locus_tag="JQG95_003370"
     CDS             complement(3890..4819)
                     /gene="ilvE"
                     /locus_tag="JQG95_003370"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY5889819.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGNVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(4839..5102)
                     /gene="ilvM"
                     /locus_tag="JQG95_003371"
     CDS             complement(4839..5102)
                     /gene="ilvM"
                     /locus_tag="JQG95_003371"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY5889820.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(5099..6745)
                     /locus_tag="JQG95_003372"
     CDS             complement(5099..6745)
                     /locus_tag="JQG95_003372"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY5889821.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(6748..6798)
                     /locus_tag="JQG95_003373"
     CDS             complement(6748..6798)
                     /locus_tag="JQG95_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889822.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(6884..6982)
                     /gene="ilvL"
                     /locus_tag="JQG95_003374"
     CDS             complement(6884..6982)
                     /gene="ilvL"
                     /locus_tag="JQG95_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY5889823.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            7334..8854
                     /locus_tag="JQG95_003375"
     CDS             7334..8854
                     /locus_tag="JQG95_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY5889824.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(8879..9217)
                     /gene="maoP"
                     /locus_tag="JQG95_003376"
     CDS             complement(8879..9217)
                     /gene="maoP"
                     /locus_tag="JQG95_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY5889825.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            9336..10175
                     /gene="hdfR"
                     /locus_tag="JQG95_003377"
     CDS             9336..10175
                     /gene="hdfR"
                     /locus_tag="JQG95_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY5889826.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(10271..10346)
                     /locus_tag="JQG95_003378"
     tRNA            complement(10271..10346)
                     /locus_tag="JQG95_003378"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10311..10313),aa:Trp,seq:cca)
BASE COUNT         2414 a         2770 c         2714 g         2454 t
ORIGIN      
        1 gcgcggtggc gttttcgatg ttggagaatc tggcagtggt gttgattgca cccgcattgc
       61 cctgcccggt gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg gtaccgttta
      121 tcatggccga tcaggggccg gtgcgccgcc gcattgagtt gtggttccga cgcaataaaa
      181 tcagtaaccc gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg
      241 cgctcggctg tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc cccgaaccgg
      301 tgcgtaaccg cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt gagctgggcg
      361 tgtgtgcgca gaaaaagcgg ctacatgagc cgctaattga ggcattctgg aagattttgc
      421 cgaaccatta acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat
      481 cgtagcccag ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg
      541 ccgccagtac gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc
      601 agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga
      661 agctgtagag gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat
      721 gcaacttcgc catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca
      781 tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc
      841 aggcgttttt ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca
      901 gttggcagaa tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt
      961 cacgctgttc gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt
     1021 tggcaccgga aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt
     1081 ttttcattcc cgccagcgcc agcgcgccag agggttccgc caccgcacgc acatcttcga
     1141 ataaatcctt catcgccgca cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat
     1201 actcctggca taaacggaag gtttcgtcac cgatgcgttt taccgcgaca ccttcggcga
     1261 acagtcccac gcgtggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg
     1321 cggagtcttc cgcttctacg gcgatcactt tgatttgcgg catcagttgt ttgatcagca
     1381 ccgctacgcc cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg
     1441 cgtcctgctg gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt
     1501 cgaacggcgg cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg
     1561 cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga
     1621 ctttgatgtc ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag
     1681 aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt
     1741 tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca
     1801 ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt
     1861 ccattttttg tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc
     1921 ttaaatattc ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc
     1981 ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta
     2041 ggcgcacagg gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc
     2101 ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat
     2161 gccacggttc gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc
     2221 gccgcttgcc gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa
     2281 acgaccgtcg gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt
     2341 tgggtagagc atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac
     2401 cacatcgccc gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc
     2461 gtacactttc gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttcac
     2521 gatgcagccg ttttcagcaa aattaccgta gagcaccgcc aggccgccgt ctttgctgta
     2581 ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag aatcccaacg
     2641 gcaatcctgc gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc ggaacatatt
     2701 ttttaccgcg tcatcctggg tcagcataac gtcgtattgt tccagagttt gcggcaacgt
     2761 caggccaagt acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcaccgag
     2821 aataccgata acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg
     2881 cgcaactttg cataactgcg ggactttacg cgaaagtttg tcgatatcac tcatggtgaa
     2941 gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc
     3001 catcgcgata tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgcgg
     3061 cagtgcactt tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc
     3121 ggcattgagg aacaactgct tacggtccgc gtgggttgcc agcagcgagc cgttgcccgg
     3181 ctgcgacagg cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga
     3241 gcaggagccg caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac
     3301 tttcgggtct gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga
     3361 cagtttggtt ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag
     3421 gcgcagggaa gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat
     3481 ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga
     3541 tggaagtgaa taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt
     3601 gttgaactct ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag
     3661 atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat
     3721 cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc
     3781 catattacga ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc
     3841 agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa
     3901 cttgatctaa ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt
     3961 gctgaatgcg tttggtaacc gggccacaac ggccttcgcc aatctgaata ccgtctacgc
     4021 tgcgcactgg cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaagtaca
     4081 gggattcgcg cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga
     4141 tgatggcatc acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca
     4201 caccatcttt cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat
     4261 ccagcgcgat accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag
     4321 agagatagtt accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc
     4381 aggaggaaac catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc
     4441 acgggaaagc ggcgataatc acatcggttg agtatcccgc tggcgggttt actcccatgc
     4501 caacattacc gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga
     4561 tcacgtcacg acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga
     4621 ttttggcgga gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt
     4681 tgtgtgagtc gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg
     4741 acatcacatg caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat
     4801 cagctttctt cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat
     4861 gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta
     4921 aacagtaagt cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca
     4981 tcgctggcgg cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt
     5041 aaaacacgtt ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc
     5101 atgataattt ctccaacatt tctgaattac tggcacctgg cggcaccagc ggccagacgt
     5161 tctcaagttc gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt
     5221 cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg gccagggatg ccgaaggcgc
     5281 tggctaacat gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgaa
     5341 aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt
     5401 tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc
     5461 cgtcaccgga gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg ccaactgccg
     5521 ccggtaaacc aaaacccatg gtacctaagc cgctggaggt gatgaaattt tccgggcgag
     5581 tgtgggcgat gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc
     5641 aatccgcagg tttacgatcc gacagttgtt ttaacaacaa cggagcgtag atagcgtcac
     5701 cgggatggtc gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt tgctgccagt
     5761 cattgatatt taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg
     5821 ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac
     5881 tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca
     5941 cggcgatcag caggtcacac tcctgcaccg cgaagtttgc cgctttggtt ccgtgcatcc
     6001 ccagcatgcc cagatagtac ggataatctg cttctactgc gcccagccct ttcagcgtac
     6061 aggtggcagg catttttgtg gcagcgagaa attcacgcaa agccggaact gcctgcgcca
     6121 tccccacgcc accgccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg
     6181 cttgctcgac ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt
     6241 ccagatcgcc gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac
     6301 gacctgagct ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct tccagagact
     6361 gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa
     6421 atgcgtcggt gccgataaac ggtgcggaca cctgaccagt gatggcaacg acagggatgg
     6481 aatctaacag tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg
     6541 cgatacatac gccagtttta ccggtagcac gggcataacc gatagccgcc attgccgcac
     6601 cctgctcatg tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg
     6661 gcataattgc gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat
     6721 gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa
     6781 ttttgtgctg ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaccccc
     6841 ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc
     6901 caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa
     6961 tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa
     7021 agagttacca tagattttgc gaatagcaca agatattttt gggcctattt ttatgatggc
     7081 tttaataatt tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaattc
     7141 aatttcagta taaatgttgt tcccttcctc gtagttcatc ctttgtccgc tgcatttttg
     7201 cgagcatcat tccaccgcca gaacaactca acttcacttg cacaaataaa caggaatcag
     7261 ccactaaaat tcacgaatta gcgggctaca gcagcgatat cttatgcaca taatggtgac
     7321 tcaaggaggg tttatgtcac tgtcaattgt tcatacccgc gcagccctgg aagtaaatgc
     7381 gcccccaatc actgttgagg tacatatcag taaaggtcta cccggcttaa cgatggtggg
     7441 cttaacagaa acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag
     7501 cggatatgaa tatccggcga aaaaaatcac catcaacctt gctccagccg atctgccaaa
     7561 agaaggggga cgatatgatt tacctatcgc cattgcgttg ctggcggcct cagaacagct
     7621 tacagccaat aagttagatg aatatgaatt agtcggagaa ctagcgctta caggcgctct
     7681 gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct attaagtcgg gcagaaaaat
     7741 tatcgtcgcg aaagataacg aagatgaagt ggggctaatt aacggtgaag gatgcctgat
     7801 agccgatcat ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc tcgaacgccc
     7861 gaaaccaact gatgcagtat cccgggcgct acaacatgat ctcagtgatg ttgtcggtca
     7921 ggagcaagga aaacgaggac tggaaattac cgccgctggt gggcacaacc ttttactgat
     7981 tgggccgccg ggaacaggta aaacaatgct cgccagccgt attaatggcc tgttgccaga
     8041 tttaagcaat gaagaggcac tggagagcgc tgcgatatta agtctggtaa atgctgaatc
     8101 agtacaaaaa caatggcggc agcgcccgtt ccgctcacct catcacagtg cgtcgttaac
     8161 tgcgatggtg ggcggtggtg caattccagg gccaggtgaa atttcgttgg cgcataacgg
     8221 cgtgcttttt cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga
     8281 gccgattgaa tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc
     8341 ccgtttccag cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca
     8401 taaccgctgc acgccagaac agacattacg ttatctcaac cggctctcgg ggccctttct
     8461 cgaccgcttc gatctctcac tggagatccc attaccaccc cccggcattt tgagtaaaac
     8521 ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc gtaatggccg ccagagagcg
     8581 ccaatttaag cggcagaata aactgaatgc ctggctggat agtccggaaa tacgccaatt
     8641 ctgcaaactt gagagcgaag atgcgatgtg gttggaagaa acgctgatcc atctggggtt
     8701 atcgattcgt gcctggcagc ggttactgaa agttgcacga accattgctg atattgatca
     8761 gtctgacatt atcacacgtc agcatttgca ggaggcagtt agctatcgtg cgattgaccg
     8821 tttgctcatc catctgcaaa aactactgac ataaaaaaag ggcatttcgc cctttttatt
     8881 aatcgtcaga atcggtgtag tcttcagcac cttcaacctg cggtttaccg ccggaaaggg
     8941 tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt ggaccacacg cgttctgctt
     9001 ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa gagtttctct tcctcggtaa
     9061 ccggctcgcg tttgccaaga tccaactcat tgaaggcata accatgacgc tcaagcagtt
     9121 gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc acgtggataa tgtttattgt
     9181 cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct gcacgctcct aattctttga
     9241 ccgagctagt tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa caaaacattt
     9301 aaatcataac gacaaataat tttgcggaga gcactgtgga tacggaattg ttaaaaactt
     9361 tcctggaagt tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc
     9421 agtcagcagt gagctttcga atcagacaac tggaaaatca actgggtgtg aaccttttca
     9481 cccgccacag aaacaatatc cgtttaaccg ctgccggtga aaaactactg ccttatgcag
     9541 aaacgctcat gagcacctgg caggccgccc gcaaggaggt ggcgcatacc tcacgacata
     9601 acgagttttc tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat cagtggttgg
     9661 gacgcttgta tcaaaatcag gatgcccata caggcttaca gttcgaagcg cgaattgccc
     9721 aacggcagtc tctggtaaaa cagctgcatg aacgccagct tgatcttctt attaccactg
     9781 aagcgcccaa aatggacgaa tttagtagtc agttgctggg gtatttcact ttagcgcttt
     9841 ataccagtgc cccttctaaa ctaaagggag atcttaacta tctgcgactg gagtgggggc
     9901 cagattttca acagcatgag gcaggtttga tcggtgctga cgaagttccc attctgacaa
     9961 ccagttctgc tgaactggca cagcaacaga ttgcgatgct taatggttgc acctggctac
    10021 ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt tgtcgatagc acaacacttt
    10081 cacggccgct ttatgccata tggctgcaaa atagcgataa aaatgcgttg attcgcgatc
    10141 ttttgaaaat taacgtgctg gatgaagtgt attaatctga atggctggca aggatgccgg
    10201 tagaaggatt tacttcggag aggattattt cagataaaaa aaatccttag ctttcgctaa
    10261 ggatgatttc tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg
    10321 tgctctacca attgaactac gcccctaatt ag
//
LOCUS       DADLCQ010000135        10294 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000135 DADLCQ010000000
VERSION     DADLCQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.136"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..401
                     /locus_tag="JQG95_003379"
                     /pseudo
     CDS             1..401
                     /locus_tag="JQG95_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            484..741
                     /locus_tag="JQG95_003380"
     CDS             484..741
                     /locus_tag="JQG95_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889827.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JQG95_003381"
     CDS             complement(1299..2066)
                     /gene="fecE"
                     /locus_tag="JQG95_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY5889828.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JQG95_003382"
     CDS             complement(2067..3023)
                     /gene="fecD"
                     /locus_tag="JQG95_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY5889829.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JQG95_003383"
     CDS             complement(3020..4018)
                     /gene="fecC"
                     /locus_tag="JQG95_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY5889830.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JQG95_003384"
     CDS             complement(4015..4917)
                     /gene="fecB"
                     /locus_tag="JQG95_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY5889831.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JQG95_003385"
     CDS             complement(4962..7286)
                     /gene="fecA"
                     /locus_tag="JQG95_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY5889832.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JQG95_003386"
     CDS             complement(7373..8326)
                     /gene="fecR"
                     /locus_tag="JQG95_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY5889833.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8323..8844)
                     /locus_tag="JQG95_003387"
     CDS             complement(8323..8844)
                     /locus_tag="JQG95_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY5889834.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9120..9324
                     /locus_tag="JQG95_003388"
                     /pseudo
     CDS             9120..9324
                     /locus_tag="JQG95_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9318..9422
                     /locus_tag="JQG95_003389"
                     /pseudo
     CDS             9318..9422
                     /locus_tag="JQG95_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9762..10019
                     /locus_tag="JQG95_003390"
     CDS             9762..10019
                     /locus_tag="JQG95_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY5889835.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2401 a         2734 c         2822 g         2337 t
ORIGIN      
        1 tcgttctcgc cggacagcag gcttaccatg aaagcactgg aaatggcatg ggaaacccgt
       61 ggtaagcccg tcggggtgat gttccaagcg atcaaggcag tcattatacg agcaggcagt
      121 tccggcagtt actgtggcga taccagatca gacagagtat gagccggcgt ggaaactgct
      181 gggataacag cccaatggag cgcttcttca ggagtctgaa gaactaatgg gtgccagcga
      241 cgggctatgt aagcttcagc gatgcagctc acgcaataac ggactatatc gttggatatt
      301 acagcgcact aagaccgcac gaatataatg gtgggttacc accaaacgaa tcagaaaacc
      361 gatactggaa aaactctaac gcggaggcca gttttagttg accacaacag actacctgaa
      421 gggagccgcg gtcgcctagc agttgcagta gcaggagatc atccagccgc agtacaggtc
      481 acgatgactc tggttaatga taccggcttt gaccccgtat tttccggctc tatcgctgaa
      541 tcatggcgtc agcagccgtg cacaccatcc tattgttgtg actgggaggc tgccaccatg
      601 cttcgcgctt tccctctggc gaaaaaggga gaaggacggg cccgtctgcc ttcactttat
      661 gccagcttcg gtaagctggg tgagacaccg actcatgaag atatcattga taacaatcga
      721 tccatcaact ggcctgtata acgtggctgc cggtgattaa gaaagctgca cctacctaag
      781 tagtagcaaa cgcacacttt ttagaaaaat cgatggtcag aaactggatt agcaattccg
      841 ttccagggtt gcttttgatt tacgttggcg tctgatcatt gatttatcct caaaagccca
      901 acctcattgg taatgaacca gctccgtgaa tgtccgctct ggcacagagc gaaatttttt
      961 gatctccccc cctgaaatct aaacttagtc atgtcacgtt tttgggtttc taaaatttta
     1021 acttcgcgtt tttcgttgcc gtaagggtta tacagaaatg tccgttaagc agagttcaaa
     1081 attgattgcc gtgatcacga ctggtttgaa agccgcgccc aagcctgtac agctctggtt
     1141 tgcgttgatt atgaacctgt cagcctaaag caagcggatg gacgatgagt attggtaatc
     1201 tttcagagtc cggaaaagtt cagccccagt ctgaacaggc ttgctggcgc cagtccagtt
     1261 tcattcagtc gtggtttggt tcttacggcc tgtgcaatct acctcattag gcacatcggc
     1321 ctgccagata ccggctcggg gtgtatttcc gcttccacgc tgaatactgt tctcagcaat
     1381 cctggggtca tcacctcttc tggtgtgcct tgcgccataa catgtccgtt tgccattacc
     1441 accagttgat cgcagtaccg gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt
     1501 ttcccctgag tccggagttc gcccatcaac cgcatcaggt ccacctggtg attgatatca
     1561 agataggtgg ttggctcatc aagtaataca acgggcgtat tctgggccag gaccatcgcc
     1621 agaaatgcgc gctggcgctg accgccggaa agctcggtta accgacgaac ggcaagatga
     1681 ttgatccggg tctggttcat ggcgacatta actcgtgcat tgtcttcagc ggagagacgc
     1741 ccccagagtg acagccaggg attacgacca tacgaaacca gctcctggac tgtgatcccc
     1801 tctggcgtta aatggtgctg aggcagcagc gaaagcctgc gggccaactg gcgtgatgag
     1861 agcatattta tgggattatc gccgagaaat acggtgccag actgcggcat taaaagccgc
     1921 gaaaaacagt ttaacagcgt cgatttcccg caaccgttag ggccgatcag ggcggtgatc
     1981 ttccccgttg gcagtgagag tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc
     2041 gtcagatttt cagttcgtaa agtcatttat cgcattctca caagcaacca gacaaaccac
     2101 ggcgcaccga taatggcggt cagcacgcca accgggagct ccagtggggg atgaataatt
     2161 ctcgccagca gatcggcaac caccaacagc aacgcacctg tcagggctga aacaggcagc
     2221 agtctgcggt gacgtccacc ggtgatgcta cgcatcatat gcggcaccac gagaccaata
     2281 aagctaatcg ggccgcaggc ggccacgccg gtagatgtca tggcgacagc tagtaacaaa
     2341 gcccagaatc gggtatgggg caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt
     2401 gcaaggaggt cgagatcgcg gcaaaaactc aggctcagcg gcagaaataa aatcatcagc
     2461 gggatggcaa tcttcacaaa gctccagtca cggccccata agctgccggt cagccacagc
     2521 agggcgttgt tcacatcctg cgggcgcgag agcatcagat aatccgtcag gctggcccag
     2581 catgcagaaa gcgccacgcc ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc
     2641 atcttcagta atatcaaccc cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc
     2701 acgggcagtg acggcataag aagtagagcc cccacagagg ccaggctggc ggcatggtta
     2761 acgccgagaa tatccggtga tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc
     2821 gccacggcga gggctgcacc gacaaacagt gccagcagca agcgcggcag tcggtactcc
     2881 atcaatacat aataatgctc gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc
     2941 acggggatca ctcccatatg gagtgataac agcgcacagc ccgccagggc aagggtgatg
     3001 aaaataacca gcgcaatttt catcctcgcc tcctcacaag ccagacaaag caagggctgc
     3061 caatcagcgc cagcactgcg cctgcgggca gatctccggg gaaggccagc gcgcgtgcga
     3121 gtacatctgc cagcagcatc agcgtggccc ccagcagcat gctcactggc agtacgttgc
     3181 gctgatcgaa gcctgcccag aagcgcgcca gatgtggcac cagcagaccg ataaacgcca
     3241 ccggacctgc cacgctgacg cacgcaccaa ccagaagcag cactaacata ttgatgacca
     3301 aacgtagcct cgtcaggttc actcccagcg tatgggcggt gctgtcgctg aggttgagca
     3361 ggttcagttg attcgccagc agtaacacga cagggactgc agtgaccacc accggcaaga
     3421 gctgccagac atcctgccag cgggcgtggg acactccgcc tgccagccag taaaagatgc
     3481 cgtaagcatg atcttcggcc agcagcaggg tgatgcgggt aaggcccata caaaaggccg
     3541 acagcgcgat acccgcgagg atcagtttgt ttctgtcatg ggtatgacga aatccgcctc
     3601 ctgcggtcat gaccagcagc cagctcacgc cgcccccgca tgccgcgatg aacgacagag
     3661 aatagcctgc aatcggcgtc ggactcagcg cgctggtaag cgccatagcc agcgccgcgc
     3721 cgctgttaat gccgagcagt gaaggagagg ccattgggtt gtgggtcagg gtttgcagca
     3781 gcgtgcccgc gagcgccagg cttgcgccga tcagaacggc gaccaggctt cgtggcaaac
     3841 gaaggttttg caccagcgct tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg
     3901 ttgcatctgc tccggaaaca ggaatggccg agtagcaaaa cagactcagc cagaaaataa
     3961 taataagtgc tgcaacggga agcccccaca gcagcatcgg gtgttttatc gcggtcattt
     4021 cacaacggta agcggctgat ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc
     4081 agcaaaaata ccgcgcatcc gcgcccaggt gttactgtcg accgaagcaa cctgctgctt
     4141 ctgcgcggcg gttaacatct gccagagcgg atcttgttgc cagcgtttaa caatgctctc
     4201 ttcgcgatag tgggcaacca gcagccaggc aggattgacc gccagcagtt gctccaggcc
     4261 gatggacggc atggacgaac ccgccatcgc agcgggaacg ttcagcccca gagaggccag
     4321 cacgctgccg gtccaggtct cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc
     4381 aaaggccacg cgtgtccctt tgggaagctg gctggcccac tgcgccatcc tctctttatg
     4441 ttgttccaga cgtgcctgca tctctcgctt tttacccacc atttcgccga tgatagccgc
     4501 agattgcaaa ttttcagcgt aggtttcgtt gcgggactta agcagcagta ccggcgcgat
     4561 ttgctgcaag gcgatgtaaa cccccgcatg gcgactgctg tcggcaatga tcaggtctgg
     4621 tttcagagcg gcaatggctt ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg
     4681 tttcaggtgc gcacgcactt cgggcaggat gcgttttgca tcgttatcgt cggcaatacc
     4741 gatcgggctg acgtccacgg cggccagcgc atcggcgaac gagagttcca gcaccacaat
     4801 ccgttgtggc gttttttcga gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa
     4861 ggcgtggctg atcaccagca gcaggcctgc aaaaagaaaa cggataaatg ccaacataat
     4921 cacattccag ctaaaagccc ggcaagccgg gcgttaacac atcagaactt caacgacccc
     4981 tgcatataca gcgtgcgcgg ctggcctgca tagatgcctt tgttgttgtc gtcataagag
     5041 cggatgaagt agtcctggtc gaagatgttt ttcacaccga atgccaggtt cagatctgcc
     5101 atctgcgggc caaagtcata cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg
     5161 ccggtactgc cgtcggcgct ctctttcacc gtattggcgt tatccgcaaa ctggctggac
     5221 tggaaatcgc tgttcagatt gaacgtccag tttcctggct tgtagtccac gcccagcgtg
     5281 cctttatgtt tcggggagaa tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt
     5341 tccgcgttca catacgcata gctggcgtag atggaaacgt tatcaagcgt tggcgttagc
     5401 gtacccagat cgtaacgtgc ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca
     5461 gtgacggtgt cgttggtctg gttggagtcg tactgattgt taaagttaat caggaacagc
     5521 cccatttccg ccgtcagcgc gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct
     5581 ttttccggtt ccacattgcc gctttgcaca gccttgccaa tctggctgta ctgtacggtg
     5641 ccgaacgagc cttcagtgtt tgcataaaga ttccagctgt cagtcaggtg atagagcacg
     5701 ttcaacgccg gaagcggtgc gttatagctc acttcttcgt gcgtgcctgt gatggcgttg
     5761 ttctggtatg actcgatatg ttcgaaacgc atacccggcg tgatggtcca gttgccgatg
     5821 tcgattttgt catccagata ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg
     5881 taagggcttg agccggacgg caactgcccg ctgctggtgg cggtgtagta acgcatttca
     5941 tgcgttgatt cattcacata gcgatagccc acgcccactt cgtgcgcgga agggccgatc
     6001 ataaagatct ggctgtagcg tggctcaata ccgcgcaccc agtagttacg cggcgagagg
     6061 gtgatgcgtt tgccttgctc caggtagccg ctgcgcaggg tttgggtgta gaacccctga
     6121 atgttgaatt tatgctggct gtctggctgg aactggtagc ccaggctcgc cagcttgcga
     6181 cgaccccaga agcggtcata cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg
     6241 cgagacaggc caccgggcat gtcggcttca ccgtcgtaat attgcagcag gctgttgaag
     6301 gtgtgcacct catccggcgc atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg
     6361 ctgtgctcgc gccagtcact gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg
     6421 ttgtccgctg tgccgcccac catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag
     6481 gttgggctga gctgaccttc cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg
     6541 gtaacaaagt tcaccacgcc gcccacgctc tgcggtccgt aacgcaccgc accaccaccg
     6601 cgtaccacgt caatggcatc catgttgccg agcgaaatgg gagccagtga aagctgcggc
     6661 tgaccgtaag gggcgaaggg gacggggatg ccgtccatca ggacggtcga gcggctggcg
     6721 aggcgcgggt tcaggccccg gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg
     6781 ttgttttccg gcgcgctgac gccagggatg cggttaagta cctcacgcat ggtggttgcg
     6841 ccggttttgg cgaaatcctc acggcggatc acgtcacgcg cgccagcatg ttcaaatacg
     6901 tcgttttcac gcgcatcacc cagccagtcg ccgaccacgg tcagggcatc ttcttttggt
     6961 gcgggcgcgg gctccagcgt ccagctgtta tttcccagcg gttttacctg cagtccgctg
     7021 ccgtccagca gttgttgcag gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc
     7081 tgcttgccgc gcgtcaggct ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac
     7141 tgattgagcg ctttatcgag cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa
     7201 aacgagagac cggccagcgg cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt
     7261 ttacgaaaaa cgcgtaacgg cgtcatacct tccccatcat catttttgtt gtgttcagct
     7321 atgagtcgaa cgagaagggt aaaaaggaca atcgaaataa gaattatttt ccttacagtg
     7381 gtgaaatgtt tatccagtac cgcgtaatag actgaatttt aacgggaagc gtttgcgcga
     7441 taacgttcag gatcgcatcg gtatttttca gcgggaacgt cccgctcagg cgcagcccgg
     7501 caacggcggg atcgcagcgc agcacgccgt tgcggtaacg ggttagcgtg gctatcacct
     7561 cacccagcgg tttatcgctg aagctcagga tgtccttcgt ccagcttgta ctctcgtcat
     7621 ccagcggttt cactgcgcca aactcagagg cgctgaactg caggctttca ccagcgttca
     7681 cgatgcgttt ttgcgcgggg gcactggcga gaagcacttc cacagcgtgc tgctgaacgt
     7741 caagctgcgt gaaattatcc tgctggcgga cggtaaattc tgtccctaaa gcagtgagct
     7801 ggccctgacg ggtcaggaca cggaaggggc gttgcagggc atctttcgcg gtagtaatgg
     7861 cgatttcacc gtaccagagc cggacggtgc gctgatgcgc atcaaaacgc acatccgccg
     7921 cgctttgggt attcagcgtg agcagggagc catcttccag ttgctgacgg ctgactgtgc
     7981 ctttggcggt gcggtaatct gcccgcagac cttcgccggt ttccgactgc cagagctgcc
     8041 agcctccgcc agcgccgagc aacagcagta atcctttcat cacgtgacgg cgggtgaggc
     8101 gggtatcgtg caacgcccgg ctggcaacgt cgccaggcac accgccaagc tggttgcgca
     8161 ggttttcaac ctgctgccag gcccactggt tatcctgatc ctgttcatac cactgttgcc
     8221 agcgcgcttc ctgttgtggg ctgacgcgct cgccgcttag cacggcatac cagtgggaag
     8281 ctgaacgcag cgcctgacgg cgggaatcgg ttaacaaagg attcataacc catactccag
     8341 acggaacagc aggcagtgct cgacggcttt cgccacgtat tttttcacgg agctgatgga
     8401 aacaccgagt ttgtgcgcaa tctcgctgta tgtcagacca tccagttgcg aaagcagaaa
     8461 cgcttcacgt gttttgccgt ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc
     8521 gagttggctt tcgcgttcct caggtgaagg cgctcccccc tccggcataa gcgccagcat
     8581 ctccagatac gctttttcca gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc
     8641 gatagtgcag aggaaggagc gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac
     8701 ccgcaaaaaa gtgtcctggg caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt
     8761 cagccagctt ttcaaccagc cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc
     8821 tgtggtagtg gcgcggtcag acatgcggag tgcatcaaaa gttaattatc acgtagtcat
     8881 attaatatga gaatggttat cattacaatt ggaaataaaa ttgtttccaa tagacatttt
     8941 taacatgttg tttttctaag tgttataagg taggtataaa atgggatgga gcctctgctt
     9001 ctgacatgtg tcggtcagaa tgactcatga tgtggtctgc tattattgac atcctcactg
     9061 ccctaaagga tggggatttc ggtaatgatc ttacccagca atagtggaca cgcggctaag
     9121 tgagtaaact ctcagtcaga ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa
     9181 cgtaaacacc attcgcatga atttcgtagt taagatctga agcttgcaga atgcatcggt
     9241 gttactgccg gagtccgtga acttagtctg catgaatcac cacagcttta catctgtcgc
     9301 agtaaacagc aaaatcagca aacttactta attatatcga gtgcgattac aatcgctggc
     9361 gatggtacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaatcat aatctcactt
     9421 aaaacggcat ccattttaca aaagatggcc gtctgtgtat ttgtaatgaa taccttctac
     9481 atgtttagag tgcataatcc acgacaaggc gatatcctaa aaattagcta gcaggcaact
     9541 ttgatggcat agataatgtt tttagtaaaa aagagttgac tgaatataat acagcctctt
     9601 cctacataag aaggaatgtg ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca
     9661 ataatcagta ttatgtattt tatttatata gcaaaatatg cttgttacac ctgtcataat
     9721 taggcatggt aaaattattg actgaaccct gggggagaaa gatgcacagt gattattata
     9781 atatggtttt tggtgaaaaa ttggccaata ttttatatga agctaactct caattttttt
     9841 acgagagaaa tgtcatagaa gaggcagtca acgctttatt ctgtgagcgt gaaataataa
     9901 acaacaagaa cataataaag aagctgatgt tctttttgtc agatgttaat cacaccaaga
     9961 aagatgttgt tcagagcgca cttaatatta tcattgacat tacatcaggt gatatttgag
    10021 attttacata aaagataatc tctcaaggag tcttatctga aaacgaaata tatagtccct
    10081 ctcgttttgt aatatcgatt ggctttgttg aatcggttac gtaccccgga gccggtcata
    10141 ttttcaggta atacacacgc tttcagacat ttcggctttc gtcatttttg tatcatgggc
    10201 atagcttctg ctacctgacc atcataatca cgcagcttca gtgaagcagt gaccacctgt
    10261 ttaacccggt gtatcgttat gatcttatcc agca
//
LOCUS       DADLCQ010000136        10126 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000136 DADLCQ010000000
VERSION     DADLCQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.137"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            144..275
                     /locus_tag="JQG95_003391"
     CDS             144..275
                     /locus_tag="JQG95_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY5889836.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            535..1530
                     /gene="pgl"
                     /locus_tag="JQG95_003392"
     CDS             535..1530
                     /gene="pgl"
                     /locus_tag="JQG95_003392"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY5889837.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1571..2524)
                     /locus_tag="JQG95_003393"
                     /pseudo
     CDS             complement(1571..2524)
                     /locus_tag="JQG95_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2708..3760
                     /locus_tag="JQG95_003394"
     CDS             2708..3760
                     /locus_tag="JQG95_003394"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY5889838.1"
                     /translation="MKKIPCVMMRGGTSRAAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3836..5269
                     /locus_tag="JQG95_003395"
     CDS             3836..5269
                     /locus_tag="JQG95_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY5889839.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AMALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5452..7632
                     /locus_tag="JQG95_003396"
     CDS             5452..7632
                     /locus_tag="JQG95_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY5889840.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMASGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7773..9056)
                     /locus_tag="JQG95_003397"
     CDS             complement(7773..9056)
                     /locus_tag="JQG95_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY5889841.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(9191..10072)
                     /locus_tag="JQG95_003398"
     CDS             complement(9191..10072)
                     /locus_tag="JQG95_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5889842.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2461 a         2366 c         2552 g         2747 t
ORIGIN      
        1 aaatcatatc tcaatgtact gtttttttct ttaaaaaaac agtacattct attttataat
       61 gactgtgtat tcccatggaa ggagatcacc caattctccc ccccgacttt tgacgcagtg
      121 tcttgttaac agggggattg accatgctta ttggctatgt acgcgtatca acaaatgacc
      181 agaataccga tctacaacgt aatgcgttta tttgtgccgg aagtgagttg attgttgcgc
      241 gaatgggaag ttggccagaa gttgttatgg catagtgttt acggtcgttg atacaggtaa
      301 acaaaagcgg ggagagtcat actccctgaa tagtcgcgaa aatatctgtg gcattgtcaa
      361 attcgtgtcg gtgactaata ttttagtaat atgccattcg aaatggagtg ctaacttatt
      421 gatttaattg atgtgcgata ttgtatttta tcgtgttttg ttgtctgttt tttatccagt
      481 aagtgttgtt atatgtatga aagataaggg atttatcatt tttgaggtgt gttcatgaag
      541 caaacagttt atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat
      601 gaaggcgcac tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg
      661 gtcagcccgg acaaacgtta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg
      721 tatcgtattg ccccggacga tggcgcactg acctttgccg cagagtctgt gctgccgggt
      781 agtccgacgc atatttccac cgatcaccag gggcagtttg tctttgtagg ttcttacaat
      841 gcgggtaacg tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgta
      901 gtcgaggggc tggacggttg ccattccgcc aatatctcac cggacaaccg tacgctgtgg
      961 gttccggcat taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg
     1021 gtggcgcagg accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta
     1081 ttccatccaa acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc
     1141 tgggaactga aagatccgca cggcaatatc gaatgtgtcc agacgctgga tatgatgccg
     1201 gaaaacttct ccgacacccg ttgggcggct gatattcata tcaccccgga tggtcgccat
     1261 ttatacgcct gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc
     1321 agcgtgttga gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt
     1381 gattacagcg gcaagtatct gattgccgct gggcaaaaat ctcaccacat ctcggtatac
     1441 gaaattgttg gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt cgggcaggga
     1501 ccaatgtggg tggtggttaa cgcacactaa ccgctgattt aaccggcgca gtctctcctg
     1561 cgccggtgta ttaacttatc tcctgtaacg catgtctctg gcgttcgacg atattggtcc
     1621 acaaattgtc tttatcgtca gtccacaaat taatcagcaa ggaaaaaaag cgttcagctg
     1681 ccggagaaag cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt
     1741 ccaccagtgg gataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa
     1801 gcgccgaaat ccccagtcct gcctccacta gtcctaatga cgtcgaaaga tgacgcactt
     1861 cgtaaaacca gtccagcttc cagggcttgt cggctagctg ttgttctatc aacaatcggt
     1921 tgccgctgga ggaacgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa
     1981 gctacttttt ggccaatgga tggtcacgcc gacaggccag cacgaatggc tcgttgacca
     2041 gtggggtaaa atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt
     2101 cgttacacag caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa
     2161 tattgggata cagctcatta aatttaccga ttgccagcgg taaaaaataa aatactgcgg
     2221 tcggaataca cgctaatgtc accatatcac gatgataagc attcatatca cgaatattaa
     2281 aaagcgtttc atcgaatttc tttattaatt cccgcgcctc cgggagcaac ctttttcctg
     2341 cttttgttaa cgtaaccttg cgagttgtac gctcaaatag ttgaatatgt aaatcttcct
     2401 ccatcttttt tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcggcat
     2461 tgttaaatga actggactct gccagtatga caaatgcctt catacttgat aattcatgct
     2521 tcattattac tccggaaaat ggaagcgacg attttggggg gctggtcgtt aaaaatttta
     2581 actgcattta gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat
     2641 tagagatgct ttgtccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg
     2701 cattattatg aaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga gggccgcgtt
     2761 cctgttagcg gaacatttac ccgaagatca aacgcagcgc gataaaatat tgatggcaat
     2821 tatgggttcc ggtaacgatc tggaaattga cggtattggc ggcggtaatc cactgaccag
     2881 taaagtcgcc attattagcc gttccagcga tccgcgtgct gatgtcgatt atctgtttgc
     2941 tcaagttatc gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc
     3001 tggcgtaggg gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg
     3061 tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa
     3121 tggtgttgtc gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc
     3181 ggttgcgctc actttcctga atgccgctgg aacgaaaacc ggaaaagttt tcccgactga
     3241 taatcagatt gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt
     3301 cgtcattatt ccggctgaat atctgggaaa aacaggttat gaattaccgg cggaactgga
     3361 tgccgacaaa gcattattag cccgcattga atctatccgg ctacaagcgg gtaaagcaat
     3421 gggcttaggt gatgtcagta atatggttat ccctaaacct gtgcttattt ctccagcgca
     3481 gaaaggcggg gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgcgctggc
     3541 gataaccggt gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca
     3601 aatcgtccct tctgtaggat atggcaatat caatattgaa caccccagtg gtgcgctcga
     3661 cgttcattta agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac
     3721 aaccagaaaa atattttccg gtgaagttta tcttccctga aaaaattcgt tgtcaggata
     3781 aggacaatca ataaaggact tctgtatgag tcatacagaa agaacaggat tttaaatgaa
     3841 taagaaatcg ttatggaagc taattctgat attagcgatc ccatgtatta ttggttttat
     3901 gccagctccg gcaggattaa gcgaactggc gtgggtgctt tttggtattt acctggcggc
     3961 cattgtgggt ctggttatca agcctttccc ggaacctgtc gtactgttaa ttgccgttgc
     4021 tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg tttaagacca ccgccgtatt
     4081 aagcggttac tcttcaggta ctacctggct ggtgttctcg gcgtttacct taagcgccgc
     4141 atttgtgacc accggtttag gtaaacttat tgcctatctg ctgattggta aaatcggtaa
     4201 caccatgctg ggtctgggtt acgttacggt attcctcgat ctggtactgg ctccggcaac
     4261 accgtctaac accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca gcgtggcgat
     4321 ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc
     4381 catttacatg gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg cggggaacat
     4441 tctggcgctg aaaatgatca acgacattct gcacctgcaa attagctggg gtggatgggc
     4501 gctggccgcc ggattgccgg gcatcattat gctgctggtc accccgctgg taatttacac
     4561 catgtatcca ccagaaatta aaaaggtgga taacaaaacc atcgctaaag cgggccttgc
     4621 cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct
     4681 actgggctgg attttcagta agtctctggg ggttgatgaa tccactgtgg caatcgttgt
     4741 tatggcgacc atgctgctgc tgggtatcgt tacctgggaa gacgtggtta aaaataaagg
     4801 cggctggaat accttaatct ggtacggcgg tattatcggc ttaagctcct tattatcgaa
     4861 agttaaattt ttcgaatggt tagctgaagt ctttaaaaat aacctggcat ttgatggtca
     4921 cggtaacgtt gctttcttcg ttattatttt cctcagcatc atcgtgcgtt atttcttcgc
     4981 ttccggtagt gcctatatcg ttgctatgtt acctgtattt gccatgctgg cgaacgtctc
     5041 cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc tccaactcct atggcggcat
     5101 ggttactcac tatggcggcg cggcaggtcc ggtcatcttt ggcgtgggtt acaacgatat
     5161 taaatcctgg tggttggtcg gtgcggtact gacgatatta accttcttgg tgcatatcac
     5221 cctcggcgtg tggtggtgga atatgctgat cggctggaac atgctgtaaa tatactcgtc
     5281 atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa
     5341 gctcctgggg attcactcac ttgtcgcctt cctgcaactc gaattattta gagtatatcc
     5401 atttattatc tttctgcgca cttcacggtg cgcagatatc tggagcattt gatgatcaag
     5461 ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa taattgccga agaacatttc
     5521 accggcgaaa ttaaaaaaga agaagcaaaa aaaggcacta ttgcctggtc tattctctct
     5581 tctcataata cgtccggaaa tatggataaa cttaaaatta agtttgattc attagcctct
     5641 cacgatatta cctttgttgg tattgtacag accgctaaag cgtccggaat ggaacgtttc
     5701 ccgctgccgt atgtgctgac caactgccat aactcactct gcgccgtcgg cggcaccatt
     5761 aacggtgatg accatgtttt tggtttatcg gcagctcagc gttatggcgg tatttttgtg
     5821 cctccgcata ttgcggtcat ccatcaatat atgcgtgaga tgatggctag cgggggcaaa
     5881 atgatcctcg ggtcagacag ccacacccgt tacggtgcat tagggacaat ggcagtcggt
     5941 gagggtggcg gtgagttggt aaaacagctg cttaatgaca cctgggatat cgactatccg
     6001 ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt atgtggggcc acaggatgtg
     6061 gcgctggcta tcattggcgc ggtgttcaaa aacggttacg tcaaaaacaa agtcatggag
     6121 ttcgttggac cgggcgttag cgcgctctct accgatttcc gtaacagcgt tgacgtgatg
     6181 accacggaaa ccacctgttt aagctccgtc tggcaaactg atgaagaagt gcataactgg
     6241 ctggcgctgc acggtcgcgg ccaggattac tgccagctta accctcaacc gatggcgtac
     6301 tacgatggct gcatcagcgt tgatttaagc gccatcaaac caatgattgc gctgccgttc
     6361 cacccgagca acgtgtatga aatcgacaca ctgaaccaga acctgaccga cattctgcgt
     6421 gagattgaaa ttgagtccga acgcgtggcg cacggtaaag ccaaactctc gctgctggat
     6481 aaagtggaaa atggtcgcct gaaagtgcag caggggatta tcgcgggctg ttctggcggt
     6541 aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg gtcaatcctg tggcaatgac
     6601 accttctcgc tggcggttta cccgtcatca cagccggtgt ttatggatct cgccaaaaaa
     6661 ggtgtggtag cagatttgat tggcgcaggc gcaatcatca gaaccgcatt ctgtggccca
     6721 tgctttggcg cgggcgatac gccaatcaac aacggtttga gtattcgcca caccacgcgt
     6781 aacttcccga accgcgaagg ctctaagcca gccaatgggc agatgtcagc ggtggcgttg
     6841 atggacgctc gttctatcgc tgcgactgcg gcaaacggtg gctatttaac ctctgccagc
     6901 gaacttgatt gctgggacaa cgtgccggag tacgccttcg atgtaacgcc gtataaaaac
     6961 cgtgtttatc agggctttgt gaaaggggca actcagcaac cgctgattta cggaccgaac
     7021 attaaagact ggccggaatt gggtgcgctg actgacaata tcgtcctgaa agtgtgctcg
     7081 aagatcctcg acgaagtcac caccaccgac gaactgattc cttccggtga aacctcttct
     7141 tatcgttcaa atccgattgg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg
     7201 cgcattaagc agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc
     7261 atggtctatg cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag
     7321 cgtgtgattg gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct
     7381 aacgtcatca actgggggat gttaccgctg cagatggcgg aagtgccaac ctttgaagtg
     7441 ggggattaca tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt
     7501 aaaggttatg tgatccatga agatgcgccg gtaacggaaa ttacgcttta catggaaagc
     7561 ctgacggctg aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac
     7621 cgtcagatgt aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc
     7681 gatgctggcg catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt
     7741 ttacgccgca tccggcaaat agttaatagc tcttacttct tcgcctctgc aaccactttg
     7801 ctgcccacgc cgcggttatt gtattcccac atgcggttgt agttagtgtc attcagattg
     7861 cgctgtattt cgtcgttatc atctacgctg ccggtattac ccgcaaacgg acgattagag
     7921 atcaccgcat cggcccacgg tttagccgtg ttaaaacctt cgttgatggc gctatcacgg
     7981 atcaccacct gaccgttggt attagcatca acatccagcg agcggcccag ttgcgccacg
     8041 ccatcaccgg aagcattgaa acggctgttt acggcgagga aaccgtaata gatgttagac
     8101 agcgtagccg gtgcaaacac atatgcttct tgctgggtac gtgagttcac cacgcggaat
     8161 tcggtgttat cgaacaccac tgcgccgcga ccagaaacga tatccacatc cccttcaata
     8221 tagctgttgg tcaccagcgt acgcggctga cggttggttt ccagacggtt ctgcacaccg
     8281 ctgttggtga caaagaaggt gttctgacga ccgagaatgt taacgttgtt aatctgtact
     8341 tggtcaccgt cagtacgcag tgctaccgcc ggatggttac ctgcatctac gctatcgccc
     8401 agcgtgtttt cgatggtcag attttgcagt tgcagtccat tgttttgtga ccagaagacc
     8461 gcagaacaca gaacaccgat actgtcgctg cgtttgctct ggcagctatc gtacatatac
     8521 cacgctggtt tacctggcat atatttgccg cgcgggttga cgtcgtgacg ccagtcggca
     8581 gggctcatgc ccccatcaag ggaaagccca atcttcacat caatcggttt ttcacctgta
     8641 ccgtacagag tgattccacc tggagcagca gggacataca ccgttccctg atactcccca
     8701 ggcatcacgg caatatactg gcgcttgttg gtgcgcttga taattgccgc atctaccgcc
     8761 gcctgaatcg tggtatgcgt tacaccttga gtacccgccg ggccgacaac aaagtcaggt
     8821 tgcgcaggca gggttatcgg ggacggattc cacgctgcag cacctggtgt cagggatgca
     8881 aaatagtgtt gcgcatcgaa attcagcgct tcttttgccg acagaatcgg gcgagaagag
     8941 gtaccaggcg cagtttgatc agaaggacgt tgatcgggtg gtgttgagct acaggcggtc
     9001 agcgtcacgc caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt
     9061 tgctccgggc tatgaaatag aaaaatgaat ccgttgaagc ctgctttttt atactaagtt
     9121 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcat tatcagtcaa
     9181 aataaaatca ttatttgatt tcaattttgt cccactccct gcctctgtca tcacgatact
     9241 gtgatgccat ggtgtccgac ttgtgcccga gaagatgttg agcaaactta tcgcttatct
     9301 gcttctcata gagtcttgca gacaaactgc gcaactcgtg aaaggtaggt ggatcccctt
     9361 cgaaggaaag acctgatgct tttcgtgcgc gcataaaata ccttgatact gtgccggatg
     9421 aaagcggttc acgacgagta gatgcaatta tggtttctcc gccaagaatc tctttgcatt
     9481 tatcaagtgt ttccttcatt gatatcccga gagcatcaac atgcaatgtt gtagggatgg
     9541 caatttttac gcctgttttg ctttgctcga cataaagata tccatctacg atatcagacc
     9601 acttcatttc gcataaatca ccaactcgtt gcccggtaac aacagccagt tccattgcaa
     9661 gtctgagcca acatggtgat gattctgctg cttgataaat tttcaggtat tcgtcagccg
     9721 taagtcttga tctccttacc tctgattttg ctgcgcgagt ggcagcgacc gggtttgttg
     9781 ttatatggcc ttcagctatt gcctctcgga atgcatcgct cagtgttgat ctgattaact
     9841 tggctgatgc cgccttgccc tcgtctatgt atccattgag cattgccgca atttcttttg
     9901 tggtgatgtc ttcaagtgga gcatcaggca gccccctcct tattgcttta attttgctca
     9961 tgtaatttat gagtgtcttc tgcttgattc ctctgctggc gaggattttt tcgtagcgat
    10021 caagccatga atgtaacgta acagaattat cactgttgat tctcgctgtc agaggcttgt
    10081 gtttgtgtcc tgaaaataac tcaatgttgg cctgtatagc ttcagt
//
LOCUS       DADLCQ010000137         9951 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000137 DADLCQ010000000
VERSION     DADLCQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.138"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="JQG95_003399"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="JQG95_003399"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY5889843.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISKSVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="JQG95_003400"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="JQG95_003400"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY5889844.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="JQG95_003401"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="JQG95_003401"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY5889845.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="JQG95_003402"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="JQG95_003402"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY5889846.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMNVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAV
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4818
                     /gene="citX"
                     /locus_tag="JQG95_003403"
     CDS             4267..4818
                     /gene="citX"
                     /locus_tag="JQG95_003403"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY5889847.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHRVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4793..5671
                     /gene="citG"
                     /locus_tag="JQG95_003404"
     CDS             4793..5671
                     /gene="citG"
                     /locus_tag="JQG95_003404"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY5889848.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5722..7184
                     /gene="citT"
                     /locus_tag="JQG95_003405"
                     /pseudo
     CDS             5722..7184
                     /gene="citT"
                     /locus_tag="JQG95_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7298..8104
                     /gene="rna"
                     /locus_tag="JQG95_003406"
     CDS             7298..8104
                     /gene="rna"
                     /locus_tag="JQG95_003406"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY5889849.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8333..8743
                     /gene="rnk"
                     /locus_tag="JQG95_003407"
     CDS             8333..8743
                     /gene="rnk"
                     /locus_tag="JQG95_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY5889850.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8974..9951)
                     /locus_tag="JQG95_003408"
     CDS             complement(8974..9951)
                     /locus_tag="JQG95_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY5889851.1"
                     /translation="MACGDCFFCRLQQYAACENTNAGKGAALNKKQIPAPAALFGYSH
                     LYGGVPGGQAEYVRVPKGNVGPFKVPPLLSDDKALFLSDILPTAWQAAKNVQIQQGSS
                     VAVYGAGPVGLLTIACARLLGAEQIFVVDHHPYRLRFAADRYGAIPINFDEDSDPAQS
                     IIEQTAGHRGVDAVIDAVGFEAKGSTTETVLTNLKLEGSSGKALRQCIAAVRRGGIVS
                     VPGVYAGFIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMP
                     FEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2349 a         2677 c         2506 g         2419 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt aaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatgaacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg tgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ctacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg cgcgcgtgga
     4741 aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa cgatgtcgat
     4801 gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg agtacgccct
     4861 gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag gcctcgtgga
     4921 tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc gcagcgcgct
     4981 ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg cagaaatggc
     5041 accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag gtgatatgtt
     5101 ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag ggctgctatg
     5161 tgcggcaatt ggccgtttgc ttcagctcaa ccagccggtt acgccaacaa ccgtttgttc
     5221 tacggcggca agtttctgcc gtggcctgac cgatcgcgaa ctgcgtacca ataattcacg
     5281 actgacggca ggtcaacggt tgtaccagca gcttggcctt accggcgcac gcggtgaagc
     5341 cgaagcaggt tatccactgg tgatcaacca cgccttgccg cattacctca ctctgctgga
     5401 tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga tggctaccaa
     5461 cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc tacagcgcga
     5521 ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc accgatctcg attatctccg
     5581 gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg ccgacctgct
     5641 gatccttacc tggtttttag cacagattta attatttaag cacttgataa atttggaaat
     5701 attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa actattggcc
     5761 ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat gccgccgcag
     5821 gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct cgagccaatt
     5881 ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag taattacctg
     5941 ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca ggcgctgaaa
     6001 tggggcctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc atttattttt
     6061 gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct ggtgaaattc
     6121 atggacaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat tctgctggca
     6181 ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt cattaaaaac
     6241 ctgccgccgt tgtttaaatc attcccgaac gatccgtccg cgcgtcgtat tggcggctat
     6301 ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt caccggtgcg
     6361 gaccaaacgt gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt
     6421 tgcagtggtt cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc
     6481 tttcctacgt gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg
     6541 gtgatgaact aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg
     6601 tattgctcag cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg
     6661 gtctgctggc agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc
     6721 gctataacag cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg
     6781 gcctgactcg ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag
     6841 gattctcacc aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc
     6901 tgtttgccag cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta
     6961 aaggtattcc gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta
     7021 tcatgggctg tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct
     7081 atgtgaaatc aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta
     7141 tgttgctgtt ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc
     7201 tgaataaatc atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata
     7261 gatagtattc agtgtatgta tgagttccac acccattatg aaagcattct gccgtaacgc
     7321 cgcgttgctc gcggtttctc tgcttccctt atcttctgcc aacgccgtag cgttgcaggc
     7381 aaaacagtat ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg
     7441 ccagagtcaa cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac
     7501 gaccaacaaa actgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt
     7561 tgctgcccgt ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat
     7621 cccgaatcta ccagaagcac gcgccagccg aatgtgttca tcgccggaaa ccgggttatc
     7681 actggaaacg gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct
     7741 ggaacgctac gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt
     7801 cggtacgatg gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc
     7861 ggataattac ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg
     7921 gggaaaagag aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac
     7981 tgaaattcag atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt
     8041 cttgccacaa cctcacccag gtaactgtgg caaaaccttt gtgattgata aagcgggtta
     8101 ttaatcctct gatttacaaa acacgctaat tcgtcttcca taaccatgcc gattaagaat
     8161 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8221 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8281 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8341 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8401 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8461 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8521 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8581 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8641 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8701 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8761 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8821 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8881 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8941 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     9001 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     9061 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     9121 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9181 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9241 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9301 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9361 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9421 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9481 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9541 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9601 ggcgattgtc aacaatccca caggaccagc accatagact gcaacgcttg aaccttgttg
     9661 gatctgcaca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9721 tttatcatct gaaagcaaag gtggtacttt aaacggcccc acattccctt tcgggacgcg
     9781 gacatattcc gcctgcccgc caggaacgcc accatacagg tgactataac caaacaatgc
     9841 cgcgggagca ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9901 gcaggcggca tattgctgta atcgacagaa aaaacagtcg ccacaagcaa t
//
LOCUS       DADLCQ010000138         9930 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000138 DADLCQ010000000
VERSION     DADLCQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.139"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..339
                     /locus_tag="JQG95_003409"
     CDS             <1..339
                     /locus_tag="JQG95_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889852.1"
                     /translation="LSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            320..1642
                     /locus_tag="JQG95_003410"
     CDS             320..1642
                     /locus_tag="JQG95_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889853.1"
                     /translation="MNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQIN
                     VKAKLPQERSEKVEDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYS
                     AKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVP
                     KNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEA
                     HSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQ
                     SDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMG
                     CKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDL
                     FDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2321..2539
                     /locus_tag="JQG95_003411"
     CDS             2321..2539
                     /locus_tag="JQG95_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY5889854.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2757..2999
                     /locus_tag="JQG95_003412"
     CDS             2757..2999
                     /locus_tag="JQG95_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889855.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="JQG95_003413"
     CDS             complement(3329..4258)
                     /gene="fdhE"
                     /locus_tag="JQG95_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY5889856.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="JQG95_003414"
     CDS             complement(4255..4890)
                     /gene="fdoI"
                     /locus_tag="JQG95_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY5889857.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4887..5789)
                     /gene="fdxH"
                     /locus_tag="JQG95_003415"
     CDS             complement(4887..5789)
                     /gene="fdxH"
                     /locus_tag="JQG95_003415"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY5889858.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="JQG95_003416"
     CDS             complement(5802..8852)
                     /gene="fdnG"
                     /locus_tag="JQG95_003416"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8265..8267),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY5889859.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9046..9879
                     /gene="fdhD"
                     /locus_tag="JQG95_003417"
     CDS             9046..9879
                     /gene="fdhD"
                     /locus_tag="JQG95_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY5889860.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2490 a         2395 c         2544 g         2501 t
ORIGIN      
        1 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
       61 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
      121 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
      181 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
      241 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
      301 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
      361 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
      421 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
      481 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
      541 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
      601 tccgtcgatc accaataaat actcagcaaa agatgtaggt ccagatatac ttgcattgcc
      661 cgtcaacgta tatcatgcgg attttaaaat taaaatcaat aatgcagtgg agaaggcttc
      721 atcaattgtg ttatgtgctg tgcaaaaaaa tctccatgca gcaaggaagg ttcttagcgg
      781 gataatagga aatgtgccaa aaaataataa accaaaaaca attatttttg atttgcgttt
      841 tcttagtgca attaagcaga aagttttttt gggcagtgaa aaaccgttgt ttgtaaagca
      901 taccgcaatg atgagggagg catgtaagga attaccgcag tcagtggaat atgttccttt
      961 agcctgtgaa gcgcatagcc atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat
     1021 catggcgaca ggtgtgggct tggcattaat gtatgcaaca tcatcaggac cgtattatgc
     1081 gcaaagtctt tctggttcgc cggatatcga gatgataaaa aatcgcatgg ctgaattgtt
     1141 taaccagtcg gatatttctg tgcgcaacaa atatagcccg gcttttgata aatggaatga
     1201 actggtagat aaattaaatc acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat
     1261 tttagcgacg gcgataaatg aaactcttga gggggatacg aatgttgccg ttgtgatggg
     1321 gtgtaaatct gcgaaagatc gcacgatatc cattgtcctt ggcaactcga tgttgcaaac
     1381 actccttgaa aaacgcctcg ccgatagtgg agaaatagaa aatctttttg atcaacaggg
     1441 atattttagt tgcgattcat taacacccga agaattaatg atgttaaaag acctttttga
     1501 tattcgagtt cttcatgtct ctaacaaatt caatgttgga ttgcaaggta atatcaacac
     1561 tgatgtttta caagattcgt tttttaaaaa tgtcgatttc attcatgaat ataactcgta
     1621 taccgtaggg atgggagtct gacgagtaaa actcattagg attatcagaa ttcggacaat
     1681 gcattgcaaa aatgacatac aaagagagat gtgtgcaggt acttagccgc gaggggcatg
     1741 gcgaagccat acagaaagta ccagtattca gaactccagt gaaagcggca tagtggccgc
     1801 ctgctcagga gttttgctat gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga
     1861 ttaaaaaaac tcatcgtcgc gctgatgatt gacgtcaatg ctatcggatg atgccggggg
     1921 tgttccggaa ggaattcctt gtttcccgaa ctgttaccaa caaaattata acgatgagca
     1981 agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa tttcacctta
     2041 aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat tatgcaaata
     2101 aatatccctg aatataatat attcatagca cccataactc atcctgggcg ataactaaac
     2161 catttttgag taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg
     2221 caaaaacaaa atgacatgca aaaatccgca tttttttaaa tgcttttccg gcgtaatttt
     2281 cgcacataca ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt
     2341 tcttcattta tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt
     2401 ttcacgatct gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc
     2461 ggtgcgtaaa gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg
     2521 taaggagcat aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt
     2581 attctggtat taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc
     2641 gtatctggaa aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga
     2701 ttcggcaagg gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga
     2761 actctctggc aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta
     2821 agcaacttga tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag
     2881 cggtagatca atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct
     2941 ggcaagggtg tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat
     3001 gggaaaaaat gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg
     3061 tgtgaacgcc ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt
     3121 aggcctgata agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata
     3181 accttatcga tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga
     3241 atgggtaacg cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg
     3301 acctgcgcca cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt
     3361 taatggaact acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga
     3421 ggtcatctgc cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag
     3481 tgccgcagtc atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt
     3541 aatgcagttt gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg
     3601 tttcacacag gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc
     3661 tggacaccgg catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc
     3721 gggctttgcc ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga
     3781 taaacggcgc tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca
     3841 tatcttccag ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc
     3901 cgctcatttc aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat
     3961 cacgcggcag aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc
     4021 gcgcggtcag atccatctcc agcggatggt cgtacagcac cactttctgg gcgtgggcga
     4081 taagcgcagc aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac
     4141 gctcggcgcg gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat
     4201 ccgccgtacg tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc
     4261 agctttcttt tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc
     4321 ccaggcgctg gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc
     4381 ggcgtagata tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat
     4441 taacgcgaat cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat
     4501 cacgccgctc accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg
     4561 accgaagtta taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt
     4621 cttcgcccaa aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat
     4681 gatgatgaac gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg
     4741 cggtgtgccc atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct
     4801 caccgccgcc aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt
     4861 gtagcgcacg atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga
     4921 ttattctctt cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc
     4981 gcaaaggtag ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc
     5041 acggtttcgc tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca
     5101 tggtgcagca cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca
     5161 ttgtcgtaac cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac
     5221 tctttcgtgc cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc
     5281 ccaaccacca cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg
     5341 aggcgcggaa tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg
     5401 gactggaagt cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct
     5461 ttcaggcagc ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt
     5521 ttgtcgttct gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca
     5581 ttggggttgt cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct
     5641 gaacaagcca cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc
     5701 acttcttcct ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga
     5761 cgaatgatat cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc
     5821 acaaggaagg acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa
     5881 gtattggcaa taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg
     5941 atggtatcga tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct
     6001 ttggctttga tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca
     6061 agtttattcg ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc
     6121 aacagcgcgt gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca
     6181 tacgggaact tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagct
     6241 gggttcgaga taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg
     6301 tagtgttccg ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct
     6361 tcctgctgca tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga
     6421 atatcccagc cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac
     6481 gggttaccct gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat
     6541 gcccatgccc agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc
     6601 atctggttgc cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta
     6661 ccgtcatcgc gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca
     6721 ccggttgccg gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct
     6781 tccgacttcg gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg
     6841 ttcgcgccac cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc
     6901 tcaccgtcag tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag
     6961 cgacctgagt taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac
     7021 acttcggtct ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag
     7081 ttagaggttt cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa
     7141 ccgatcactt tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag
     7201 ccattgacct tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc
     7261 cacttcggca accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag
     7321 gccttcatca tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc
     7381 agcagcggtt ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt
     7441 ggcagcgtca tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga
     7501 atattggagt gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc
     7561 agcagctgga tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag
     7621 ccgagggcat acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg
     7681 cagactttca ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg
     7741 tagcgggaaa cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta
     7801 tcgcgtttgg cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg
     7861 cgtttttccg cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg
     7921 atcaggctgg cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc
     7981 aggtacagca atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag
     8041 tagtcagcta ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg
     8101 ttgtgaattt tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta
     8161 ccgcccatca ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca
     8221 ccgcgaccaa atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg
     8281 ttgtcgaccg cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg
     8341 ttactcgacg cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct
     8401 tcggcgtttt gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga
     8461 tcaaacgcct cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc
     8521 ggaaacttca gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa
     8581 agtgcaccgc ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg
     8641 gcgttttttg ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag
     8701 gtgcaggtat tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt
     8761 gctacgctgg gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag
     8821 atcttaaaga actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac
     8881 gtcccattta cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa
     8941 ggggccgcaa aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag
     9001 aataccataa tgttggtgtg tatgttctta tctggttaag agaaagtgaa aaaaacacag
     9061 cgaaaagaaa tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg
     9121 cgtgatgatt tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg
     9181 gtctacaacg gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt
     9241 gcgctcggtt tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg
     9301 gatgtcgttc cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt
     9361 atggggttga aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc
     9421 gtggagcaac ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt
     9481 gatctcaaca aactggatga tgcattacgt catctcaacg attttcagcc agtggggcga
     9541 ctgactggtt gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg
     9601 catgaagacg tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa
     9661 ggggaaagct ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg
     9721 caaaagtcgg cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt
     9781 gctgtagaag tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg
     9841 gcaacggttt atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat
     9901 atattaaatt gcaggactta ttcatttcgt
//
LOCUS       DADLCQ010000139         9910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000139 DADLCQ010000000
VERSION     DADLCQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.140"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(165..905)
                     /gene="pflA"
                     /locus_tag="JQG95_003418"
     CDS             complement(165..905)
                     /gene="pflA"
                     /locus_tag="JQG95_003418"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY5889861.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="JQG95_003419"
     CDS             complement(1097..3379)
                     /gene="pflB"
                     /locus_tag="JQG95_003419"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY5889862.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3434..4291)
                     /gene="focA"
                     /locus_tag="JQG95_003420"
     CDS             complement(3434..4291)
                     /gene="focA"
                     /locus_tag="JQG95_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY5889863.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4697..6457)
                     /locus_tag="JQG95_003421"
     CDS             complement(4697..6457)
                     /locus_tag="JQG95_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY5889864.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEDFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6700..7213
                     /locus_tag="JQG95_003422"
                     /pseudo
     CDS             6700..7213
                     /locus_tag="JQG95_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7412..8500
                     /gene="serC"
                     /locus_tag="JQG95_003423"
     CDS             7412..8500
                     /gene="serC"
                     /locus_tag="JQG95_003423"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY5889865.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8571..9854
                     /gene="aroA"
                     /locus_tag="JQG95_003424"
     CDS             8571..9854
                     /gene="aroA"
                     /locus_tag="JQG95_003424"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY5889866.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2521 a         2369 c         2484 g         2536 t
ORIGIN      
        1 cggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta tctcaggtac
       61 gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat gaagcgcgga
      121 ataaaaaagc ggcaactcaa taaagttgcc gctttacgag gaaattagaa cattacctta
      181 tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt cggtggttta
      241 acaccatcga gtttgtattc ttcacccatc gccacccatt tgtgtttgcc cagttcgtgg
      301 taggggagaa gttcgatttt ctcaacgtta cccatatcac gggtaaattc accgaggcga
      361 tgtgccgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc
      421 acatttttgt tcgccagata tttagcgaac tccagcgtgc ggtggttgga aactccaacc
      481 agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact
      541 tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt atccagacag
      601 gtatgaatgc cttctttttt acaggcgcgg aaccagtcac gaacaaactc agcttgcagg
      661 attgcttcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag
      721 gtcaccactt ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag
      781 gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag
      841 cgaatacctg ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact
      901 gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa
      961 ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct
     1021 ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga
     1081 tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct
     1141 gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg
     1201 gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt
     1261 tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag
     1321 ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg
     1381 tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg
     1441 ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc
     1501 cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga
     1561 tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct
     1621 tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa
     1681 actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg
     1741 gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga
     1801 taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt
     1861 agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca
     1921 gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagctcagg agatcgcctt
     1981 tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga
     2041 ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac
     2101 caacgatcat cgggcttacg cagcaagcga tagcgtagtc atcgttgttg aagtccggac
     2161 gcatcaggtc atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga
     2221 atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag
     2281 acggccccat agtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac
     2341 gaccatcgag gcccatacca ccgatagatt cggttgccca gattgggtcg ccagagaaca
     2401 gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt
     2461 caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt
     2521 acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa
     2581 cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac
     2641 cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct
     2701 gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat
     2761 cagcctgtag agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca
     2821 gcgcaacgcg acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg
     2881 tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct
     2941 ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt
     3001 tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt
     3061 tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttattg atgtagccag
     3121 cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa atcaactggc gcgtgagtgc
     3181 ggttttccag tttaacacct tccattactt tgtcccacag ggtggtggtc gcttcagtag
     3241 cgccagccag gaaggactcg tcaccctcgt acggcgtgta gtttttctga atgaagtcac
     3301 ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact
     3361 tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt
     3421 acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc
     3481 caaccaacaa accaccgccg ataatattac cgatcgtaac cgggatcagg ttatcagtga
     3541 tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga
     3601 attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga
     3661 tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt
     3721 tgtccatcag gctgcggcca gaataactca tccatactgc cagacatacc atcaggtttg
     3781 ccaggatacc aagacagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt
     3841 gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta
     3901 aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttag
     3961 ccaactgaca ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag
     4021 taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc
     4081 caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga
     4141 agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg
     4201 gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa
     4261 gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac
     4321 gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta
     4381 taggcccgta actcgcatgg tttttatgca aatacggagt aactatttga ttatccaaat
     4441 aaaaataaat tttaaaaatt aacaaatgag ttgaattttt tccgcatcct ccgctaaaac
     4501 agttaattaa aagggagcat caggcgaata aagtaacaat atcgatcgta tttattaaat
     4561 acaaattacc gatatttaac cttataatta caattatttt attaatgcaa atatatgtaa
     4621 agcggggcat taaaaaaacg ccccgtaata taactcagac taatcattaa gcctacattg
     4681 cgtaggctat ttgattttat tttgcccaga atgctgcttt ggcgcgctgc agcttttcgt
     4741 aggccttcaa caacgactga tgtgcagcaa acgcgtgcag atcgctatct accggttgca
     4801 ggccgtaaaa cgccgcttcg ccgctcattg ccgcactggc ggcttctacg gcatctgcgc
     4861 cgtacatgcg aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgtgcca
     4921 gtaataacaa cgtttgcagg cagcgataat agttggcgcg ttccggacta aatactgatg
     4981 agttaaactc catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca
     5041 gcgccagcat ggcttttaat tcaccgatac gcagggtgta ccaaccgtta tccgacccgg
     5101 tcgccagacc caacagctca cgcacgcggg taaagtcatc aaaatcttct tcatccagtt
     5161 gctcgatgag gttcaggtaa tcttcttttt cccactcgct gcctggtagc gaaagaatcg
     5221 tttcacgtaa atggctgccc atactgttat tcgcgagcca cagatcttca gccggataaa
     5281 tatcggacat gccaggcacg ataatacggc aagcataaac gcccagatgc tcgtaatcgg
     5341 caatataaac ttctttatct tctttgttga agatagccat cagcgtagca aactcttctt
     5401 ccgtggtgcc ggagaaattc cagtccacaa acggataatc ggcatcctgc ttgaacaggt
     5461 cccaggagat taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga
     5521 cttcttcatc atcgaaggtt ggcggagtaa acacatccaa atctttcagg ccacgacctt
     5581 gcagcagctc ggtcacggta cgttccagtg ctacgccaaa atcaggatgc gcaccgaaag
     5641 aggcaaagca ggtgccgttt gcaggattaa acagtaccac gcaaatcacc ggatactggc
     5701 cgccaaggga accatcatat gcgaagatcg ggaaaccttc tgtttccagt gtttcgatcg
     5761 cttcaaccac cgctgggtaa cgcgccagca cgtctgccgg gatttccggc aagctgatgc
     5821 tttcagcaat aatgcggttt ttcacgtagc gttcgaaaac ttcggacaac ccctgaacgc
     5881 gtgcttcgtt gcgggtattg ccagcggaca taccgttaga aacgtacagg ttaccaatga
     5941 tattcatcgg aatataaacg gtctgattgt cggactggcg cgtaaacggc agaccgcaaa
     6001 taccacgatc ttcgttaccg gattgtaggt caatcagcat actgccggtc aattcattct
     6061 ccggatcgta aaatgcgcgc agacggtcat cgagcagccc ttctggcaca tcgtcatttt
     6121 cgctcagtgg gaaccatttt tcgttgggat aatgcacaaa tggaccgttg gcgatggttt
     6181 cgcccagcca gaaatcagca aaaaagtagt tggttgagag acgctcgaaa tattcaccga
     6241 gtgcagaagc cagcgccgct ttcttggttg cgcctttacc gttggtaaaa cacagtgcgc
     6301 actctttgtc gcgaatatgt acagaccaga cgttaggcac gggattcagc caggaggcct
     6361 cttcaatctg aaagccgagg tctgaaagtt tttgctggaa gcgagcgatg gaatcttcca
     6421 gagcggcatc tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga
     6481 aagcgcgcaa taatacgggt tttatctcaa aggcgctatc accgccgcca tgccgatgaa
     6541 ctgttgacta tgcttttagc ggataaacca cgttaagagc ataaaaatga aggcgttcga
     6601 tctccaccgt atggcatttg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg
     6661 tagtctttat acctttgtac tggttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     6721 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     6781 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     6841 ttgttatacc gcctggtaat atgatggcga gctggcctgg tcgaaactca ataactccaa
     6901 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     6961 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     7021 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     7081 gccggagtcg gcggactatg cctgcattcg ttgtagtgaa atcattcata tgaacgcggg
     7141 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     7201 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     7261 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     7321 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     7381 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     7441 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     7501 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     7561 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     7621 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     7681 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     7741 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     7801 ggttaagcca atgcgtgaat ggcaactctc tgataatgcg gcttatatgc attactgccc
     7861 gaatgaaacc atcgacggta tcgccatcga cgagacgcca gacttcggca aagatgtggt
     7921 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     7981 tgtaatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     8041 tcgtgaagat ttgctgggca aagcgaatat cgcgtgtccg tcgattctgg attattccat
     8101 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     8161 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     8221 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     8281 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     8341 caaattgttc cttgaagagt cttttgctgc aggccttcat gcactgaaag gacaccgtgt
     8401 ggtcggcgga atgcgcgcgt ctatttataa tgccatgccg ctggaaggcg ttaaagcgct
     8461 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     8521 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     8581 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     8641 tttctaaccg cgctttattg cttgcggcat tagcacacgg caaaacagta ttaaccaatc
     8701 tgctggatag cgacgacgtg cgccatatgc tgaatgcatt aacagcgtta gggttaagct
     8761 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     8821 cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     8881 cagctctttg tctggatagc aatgatattg tgctgaccgg tgagccgcgt atgaaagagc
     8941 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     9001 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     9061 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     9121 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     9181 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     9241 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     9301 cttcggcttc ttacttcctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     9361 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     9421 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     9481 ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg gcggcgttat
     9541 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gagaccgatc
     9601 gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa gaggggcacg
     9661 attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     9721 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     9781 ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg gcgcggatta
     9841 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     9901 cggcacattg
//
LOCUS       DADLCQ010000140         9643 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000140 DADLCQ010000000
VERSION     DADLCQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.142"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(384..1361)
                     /gene="epmA"
                     /locus_tag="JQG95_003425"
     CDS             complement(384..1361)
                     /gene="epmA"
                     /locus_tag="JQG95_003425"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY5889867.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1686..3494
                     /gene="frdA"
                     /locus_tag="JQG95_003426"
     CDS             1686..3494
                     /gene="frdA"
                     /locus_tag="JQG95_003426"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY5889868.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3487..4221
                     /locus_tag="JQG95_003427"
     CDS             3487..4221
                     /locus_tag="JQG95_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY5889869.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4232..4627
                     /gene="frdC"
                     /locus_tag="JQG95_003428"
     CDS             4232..4627
                     /gene="frdC"
                     /locus_tag="JQG95_003428"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY5889870.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4638..4997
                     /gene="frdD"
                     /locus_tag="JQG95_003429"
     CDS             4638..4997
                     /gene="frdD"
                     /locus_tag="JQG95_003429"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY5889871.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5060..6193
                     /gene="blaEC"
                     /locus_tag="JQG95_003430"
     CDS             5060..6193
                     /gene="blaEC"
                     /locus_tag="JQG95_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY5889872.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6282..6815
                     /locus_tag="JQG95_003431"
     CDS             6282..6815
                     /locus_tag="JQG95_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY5889873.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6812..7129)
                     /locus_tag="JQG95_003432"
     CDS             complement(6812..7129)
                     /locus_tag="JQG95_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY5889874.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="JQG95_003433"
     CDS             complement(7305..7451)
                     /gene="ecnB"
                     /locus_tag="JQG95_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY5889875.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="JQG95_003434"
     CDS             complement(7562..7687)
                     /gene="ecnA"
                     /locus_tag="JQG95_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY5889876.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7739..8305)
                     /gene="efp"
                     /locus_tag="JQG95_003435"
     CDS             complement(7739..8305)
                     /gene="efp"
                     /locus_tag="JQG95_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY5889877.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8347..9375
                     /gene="epmB"
                     /locus_tag="JQG95_003436"
     CDS             8347..9375
                     /gene="epmB"
                     /locus_tag="JQG95_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY5889878.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2451 a         2473 c         2459 g         2260 t
ORIGIN      
        1 taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata
       61 acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat
      121 caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat
      181 ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg
      241 taaaaaccaa tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc
      301 ctttaacgga aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct
      361 gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc
      421 agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa
      481 cagtcaggca taccaacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc
      541 aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca
      601 tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt
      661 tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag
      721 tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc
      781 aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc
      841 gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc
      901 aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag
      961 agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc
     1021 atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc
     1081 tggaataccg gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc
     1141 attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg
     1201 accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact
     1261 ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta
     1321 ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt
     1381 gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa
     1441 acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca
     1501 tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga
     1561 cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac
     1621 cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga
     1681 atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac
     1741 gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag
     1801 tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg
     1861 atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg
     1921 agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac
     1981 tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg
     2041 gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca
     2101 cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc
     2161 tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag
     2221 gcacgttggt gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg
     2281 tttatcgtta caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa
     2341 gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag
     2401 gttccggtat cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa
     2461 atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga
     2521 aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat
     2581 ggcgtaaagg caacaccatc cccacgccgc gtggtgatgt ggtttatctc gacctgcgtc
     2641 acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt
     2701 acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca
     2761 tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg
     2821 gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg
     2881 aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg
     2941 gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag
     3001 atctagttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg
     3061 caatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca
     3121 agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt
     3181 tcaacaccga cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta
     3241 tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag
     3301 gttgcaccga gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg
     3361 atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac
     3421 gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga
     3481 aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc
     3541 gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg
     3601 gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc
     3661 cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt
     3721 aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg
     3781 attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa
     3841 ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg
     3901 cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg
     3961 tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat
     4021 cgttataacg aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc
     4081 cagaacggtg tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac
     4141 gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc
     4201 gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg
     4261 gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga
     4321 aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct
     4381 gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt
     4441 gatcattaac ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact
     4501 ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat
     4561 caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta
     4621 ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct
     4681 ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc
     4741 tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg
     4801 ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc
     4861 cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc
     4921 ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg
     4981 gtgtcgttac aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt
     5041 tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc
     5101 ttactccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc
     5161 gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc
     5221 ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac
     5281 gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat
     5341 tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc
     5401 taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc
     5461 attgcaggtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg
     5521 gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt
     5581 cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt
     5641 cttccagcca ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa
     5701 ttacgcctgg ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc
     5761 tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt
     5821 accccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg cacaatctcg
     5881 ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt
     5941 caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag cacgcccggt
     6001 aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata aaacgggggc
     6061 gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat
     6121 gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc agattctcaa
     6181 cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg
     6241 ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg
     6301 ttgccgcagc gacagctgca tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg
     6361 gcgtgaccgt agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg
     6421 cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc
     6481 gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc
     6541 agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt
     6601 cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc
     6661 atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca
     6721 tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca
     6781 gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa
     6841 tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac tcaccgagca
     6901 gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg
     6961 tccctaccgg taacgatttc atcgcccagg caagtagcgc catactgact atcatcgccg
     7021 tcacggtaat aacactcggc gtcaaacggc taaagccgtg ggtatatttc aggccaacgg
     7081 cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg
     7141 gaaatttggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg
     7201 gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattaccttt atgacgatcg
     7261 cagaaaaaac aaacggcacg acactgctgt cgtaccgtta ttgcttattg ctgcgctttc
     7321 gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg
     7381 gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc
     7441 ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta
     7501 ataatagaca acatcgccaa aaatgatgga aatctgatga tttttcggaa gagaagaaaa
     7561 attagctggc agcgcgagag atggagttac cgagatgttt gatgtcttcg ccgaaaccgc
     7621 aagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt
     7681 tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca caaccgcatt
     7741 acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgattt
     7801 gtacgaacag cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag
     7861 tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt
     7921 tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat
     7981 ccagcagcca tttagcattg tcgccaattg cttttgcatc agcagacagc tgctcgaaag
     8041 tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat
     8101 cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct acgcgagtac
     8161 cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga
     8221 attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa
     8281 aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg gtagctaagc
     8341 cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg gttaacgcaa
     8401 cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat agacgcggac
     8461 gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca
     8521 tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca ggtacttacc
     8581 tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccactgga agaacagcac
     8641 agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct ggtcaaaggc
     8701 ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc cgaaaatcag
     8761 ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc ggaactggac
     8821 gagatgattt tctccggcgg cgatccgctg atggcgaaag agcacgagct ggactggttg
     8881 ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag ccgtctgccg
     8941 attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg ttctacgctg
     9001 caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac gttccgtcag
     9061 gcgatggcta aattgcgccg tgtcggtgtc accctgctta accagagcgt tctgttacgt
     9121 ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt cgatgccggc
     9181 gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca ttttatggtg
     9241 agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc gggatatctg
     9301 gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct ggatctccag
     9361 ctacgccagc agtaactgat taaacaggga agttaaacat taaaattgcc tgatgcgcta
     9421 cgcttatcag gcctacatcg tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg
     9481 gataaggcgt ttacgccgca tccggcaaga gcaacaaacg caagtacagc aacctgtcat
     9541 taaactgaga aatttaatct ttgcacgttg cataaccttt aattattcat atgcattgct
     9601 gttgttactt tttatatagc taacaccaat aattaacctt tct
//
LOCUS       DADLCQ010000141         9625 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000141 DADLCQ010000000
VERSION     DADLCQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.143"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JQG95_003437"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="JQG95_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY5889879.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JQG95_003438"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="JQG95_003438"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY5889880.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="JQG95_003439"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="JQG95_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4133)
                     /gene="rhaR"
                     /locus_tag="JQG95_003440"
     CDS             complement(3195..4133)
                     /gene="rhaR"
                     /locus_tag="JQG95_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY5889881.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JQG95_003441"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="JQG95_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY5889882.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="JQG95_003442"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="JQG95_003442"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY5889883.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="JQG95_003443"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="JQG95_003443"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY5889884.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="JQG95_003444"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="JQG95_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="JQG95_003445"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="JQG95_003445"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY5889885.1"
                     /translation="MIRKAFVMQVNPDAHEEYQSRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         2352 a         2515 c         2489 g         2269 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataagacca ttgtttttat ttttaaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa actacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacgtca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagag tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaat
//
LOCUS       DADLCQ010000142         9597 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000142 DADLCQ010000000
VERSION     DADLCQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.144"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="JQG95_003446"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="JQG95_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY5889886.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..1862)
                     /gene="phoA"
                     /locus_tag="JQG95_003447"
     CDS             complement(447..1862)
                     /gene="phoA"
                     /locus_tag="JQG95_003447"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY5889887.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="JQG95_003448"
     CDS             complement(1963..2223)
                     /gene="iraP"
                     /locus_tag="JQG95_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY5889888.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2405..2608)
                     /locus_tag="JQG95_003449"
     CDS             complement(2405..2608)
                     /locus_tag="JQG95_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889889.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2686..3780
                     /locus_tag="JQG95_003450"
     CDS             2686..3780
                     /locus_tag="JQG95_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY5889890.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3804..4016)
                     /locus_tag="JQG95_003451"
     CDS             complement(3804..4016)
                     /locus_tag="JQG95_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY5889891.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4276..4584
                     /locus_tag="JQG95_003452"
     CDS             4276..4584
                     /locus_tag="JQG95_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY5889892.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="JQG95_003453"
     CDS             complement(4643..5737)
                     /gene="yaiW"
                     /locus_tag="JQG95_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY5889893.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPRKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="JQG95_003454"
     CDS             complement(5750..6970)
                     /gene="sbmA"
                     /locus_tag="JQG95_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY5889894.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVAHITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7322..8479
                     /gene="ampH"
                     /locus_tag="JQG95_003455"
     CDS             7322..8479
                     /gene="ampH"
                     /locus_tag="JQG95_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY5889895.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8480..8983)
                     /locus_tag="JQG95_003456"
     CDS             complement(8480..8983)
                     /locus_tag="JQG95_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5889896.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9071..>9597)
                     /locus_tag="JQG95_003457"
     CDS             complement(9071..>9597)
                     /locus_tag="JQG95_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5889897.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2382 a         2448 c         2283 g         2484 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagcttg ttgcgttgct tgattattac attcacgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcagttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aagggttcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
      841 aggaaaaatc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgtgcc
      901 agggttggta cactgtcatt acgttgcgga ttaggcgtat aggtgactgc gggcttatcg
      961 atattgccgt ggtacgttgc tttcggccct agccagcgca ctggcatatt gccgtcagca
     1021 aacaggccaa gcaggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
     1081 tcgttcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
     1141 tcaccagcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca
     1201 cgagcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
     1261 ggacattttt cactggtcgc gctcggaccg tagcatttgc gcgaagtcac atgtgccacc
     1321 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
     1381 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
     1441 gcgccgttat aggttttgac accggttgac caggcggttg ctgatgcagc cgagtcggtg
     1501 acgtagtccg gtttgccagt ttttttattc agcgcatagt gagtgtattg cccggtaagc
     1561 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt acgtgcggca
     1621 gtaatttccg aatcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
     1681 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
     1741 ggtgcagtaa tatcgccctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
     1801 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
     1861 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
     1921 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac aaggatgctt
     1981 caataacttc tttacgtaat cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc
     2041 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt ggtcattttg
     2101 cgccatattg cgaagcattg cagtgacgat aatctccaaa gcttctacct gcgcacacag
     2161 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
     2221 catgtctgta tttccttatc caaagtatgg agaagttaac attatgtttc acaacttcat
     2281 agtgtctgga agtattattt ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt
     2341 gattaccagc gaaacgtttc tcttatttaa ataattaccc acacaaaata taggctttta
     2401 attatcaacc tttattataa ataaaagcta tgaaatatat tctcttcgtt aacttataat
     2461 taagagaaaa aaactcaccg cattgccatt taagccagga ataaattagc ttcccgttca
     2521 tcatttttgt gatcttgcgc acatctttga gataacgcac tttcttcacc agtacgttgc
     2581 acgctttgtc agcagaagat tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt
     2641 cgtggtaatg tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg
     2701 gtaggaatcg tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa
     2761 aacattgtcg atgccattga taaaagtcgc ttcgacgttg tgctgctggg cattgataaa
     2821 caagggcaat ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc
     2881 catattgcgt tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat
     2941 cagcttatcg acgcgcaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt
     3001 gtccacggta cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta
     3061 ccgtttgtag gttctgatgt tctggcttca gcagcctgta tggataaaga tgtcaccaaa
     3121 cgtctgctac gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat
     3181 cgtcacaaca tcagttttgc cgaagtggag tctaaactgg ggttaccact gtttgtaaaa
     3241 ccggctaatc agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca
     3301 attgccgtcg atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa
     3361 ggtcgtgaga tcgaatgcgc agttctgggc aacgacaatc cgcaagccag cacctgtggc
     3421 gagatcgtac tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc
     3481 gcgaaagtgg tagttccggc agccattgca ccagagatca acgataagat ccgggcgatt
     3541 gccgttcagg cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta
     3601 accccagaga acgaagtggt gatcaacgag atcaacacac tgcctggctt caccaacatc
     3661 agtatgtatc cgaagctgtg gcaagccagc ggtctgggtt acaccgatct gatcacacgt
     3721 ttgattgagc tggcgctgga gcgtcacgct gcggataacg cattgaaaac cacaatgtaa
     3781 tattttaacg cccgataata ttcttattgg gcgttttcag tttcttcatc gcgacgacga
     3841 ataatcaacc ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc
     3901 tgccaacctt ttgcttcaaa tcccagtacc cccaccacgc cattaagaat gaatataagg
     3961 ccaatggcga acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca
     4021 gctccgttaa cgaaaaacaa catccttgca tattctgtaa caaccgtctg tggtgtagac
     4081 aacattttcg taattattaa taaatttaac ttaaggttgc cattttgtga ctcacaacga
     4141 agattcacgc ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc
     4201 taaatgacct gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga
     4261 cttccggagg tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat
     4321 cgcaatgcct cttcaacgcc tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt
     4381 tgggctgggg cgcaattgct gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta
     4441 atgcaggtcc aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt
     4501 ttcgggctga tcccgttttt agcaggctgc ctcatttttg cagtggtggc gctatggctg
     4561 cactggcgac atcgccgcca gtaaaattta cgttgccaga tgcggcctga aacgccttat
     4621 ctggcctgac attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc
     4681 gcttcgcaaa ccaggccgta gtcaggttgc gggtgatttt cgggctttcc agttgaatgc
     4741 caggcaacat ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt
     4801 tcttgtacag cgccgtctcc tcaaaagaga agctgtcacc tttctctaac tgacggcgaa
     4861 tttcgctgtc gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc
     4921 tgggttcttt actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg
     4981 ccttactgac tgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat
     5041 cagcaaagcg gtatatcggt gcgctatagc tggcgggata attcagtaaa tggtaagtac
     5101 caaaccacaa cccgccgcgg cgactgaaga cttcctgacg aactgtaccg tccattttcc
     5161 acggataccc tttggtatgc tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg
     5221 tgcgcaccgg attaagcgaa ccaaacagcg tctgccccat tggtaccatg ctgatcagat
     5281 cgtcaaaaat cgcgctcaat tgcttttctg tacgcaccga atctaaccgt tcgctataac
     5341 ttttgccgtt tggcgatttg atttttagcg cggtatgaac cagaaaagca ggaatgtgca
     5401 tccgttcagc acgacggtca atctcttgcc aggcgatctt gcttaagccc ggaacggccg
     5461 gatctgcctg ataatttgac tcctgctgcg ccaccgccag caccgagtag acgttttcca
     5521 gcgtcggtgc cagcccctga ctttcaaagg tggtggcgag atctttcgcc caggcatcgc
     5581 ggtctttcac gctcgcaggc atcttctgcc gcacgacgct cgctacatct atcgcttttt
     5641 cgcccttttt caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca
     5701 acaatgacag cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg
     5761 tatgggttac ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt
     5821 tgtagataga catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagcac
     5881 cgcgaacctg accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg
     5941 caacaattga cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa
     6001 tgcgggcgat gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg
     6061 cgctaaacag ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa
     6121 ccagctcttt acggtaggcg gcctctactc gctggttttt aaactccagc cccggcagtt
     6181 tgatccctac cactgccagc aatccggtcc ccatcagcga ccagacgata gcggcaatca
     6241 ccagaccata cggaatgtgc ccgataatcg gcagctccgg cacatgcgcg gagagcgtta
     6301 ccagcaccgg caggaaggcg atcaacgtca ttatggcgtt gataaaactg acgcccatat
     6361 tctccagcgt tgaagcaaaa cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga
     6421 tatgacgcag ttgttgccag ttcgccatgt aatattcgtt catcgccgta cgccagcgga
     6481 acacgtagtg actgacaaag aagttgttca gtacactgat caccactgcg atcagcgcaa
     6541 tccccagaaa gacgcccact tcgcggtaaa attgttcgat agtgacttta tgcggcgaac
     6601 ttagcgcggt ttgaatcaga tcatagaacg gcgcatacca ggcgttgacg gcgaccccga
     6661 cttccaccaa aaaccaagtg acgaagataa tcagcgcagt accgagtatt gaccagtatt
     6721 gccaacggtg cgggctgtag ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt
     6781 agtaagcgta aaaaatcagg aaatccaacg accagaaacg cgcggcgcta atcgggatct
     6841 gcccggaagc gccggtgata tgtgccaccc agtcaccccc accggcttgc cagaagataa
     6901 cggcaatcaa tgcccaaaca aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag
     6961 acttaaacat atgcactcct gctaacttct tatcgtttta acaactgctg tgtattaacc
     7021 gcatgaccgc tacaaagcgt cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg
     7081 cttagttcgc cagcctgacc ataagaattg tttcgacgga tttcacaaac caaagattgt
     7141 ttacataagc ggattaacac gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa
     7201 gatcgtgttt acgcgccgtt acgtttttaa cactattatt gcactaattc gccctttgca
     7261 atccatcaat gagtagtata aatacgctca gttaccttca ttcaatctat ggacaccacc
     7321 gttgaaacgt agtctgcttt tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca
     7381 tgcggcgcag ccgatcactg aaccggagtt tgcctctgat attgtcgatc gttatgccga
     7441 tcatattttt tacggcagcg gtgccacggg gatggcgctg gttgttatcg acggtaacca
     7501 gcgcgtcttt cgtagttatg gcgaaacgcg acctggtaat aacgttcgcc cgcagctgga
     7561 ttcggtcgtg cgtattgctt ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt
     7621 gctcgaccag gggaccgtaa agttaaacga tccgctaagt aaatatgctc cgccaggcgc
     7681 acgcgtgccg acctacaatg ggacgcccat taccctagtg aatctggcaa cccataccag
     7741 cgccctgccc cgtgaacagc ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac
     7801 gcgcgagcaa cgctggaaat acctttctac ggcgaagctg aaagccgcgc caggcagtca
     7861 ggcggcgtac tctaaccttg cgttcgacct gctggcagat gcactggcga atgcgtccgg
     7921 caagccttat acccagttgt ttgaagagca gattacccat ccattgggga tgaaagacac
     7981 cacctacacc ccctcaccgg atcagtgccg ccgtttaatg gttgctgagc gcggtgccag
     8041 tccgtgcaat aacacgctgg cggcaattgg cagcggcggc gtctattcca cgcctggcga
     8101 tatgatgcgc tggatgcagc agtatttgtc atctgatttt tatcagcgca gtaaccaggc
     8161 agatcgcatg cagacgttga tttatcagcg cgcgcagttt acgaaagtga ttggcatgga
     8221 tgtccccggc aaagcggatg cgcttggttt aggctgggta tatatggccc cgaaagaggg
     8281 tcgtccgagg attattcaga agacaggcgg tggtggcggt ttcattacct atatggcgat
     8341 gatcccgcag aagaatattg gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt
     8401 taaaaatatg agcgatggca tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt
     8461 ggttatcccc gcatcctgat taatactctg aaggcaaacg gttgatagcg accagtttgc
     8521 ctttattcat ttcgatatag ccgcctttac gcaaagcagc gagaacttct gcgacgacag
     8581 aacgcgatat gcgtgtacgt tgatggatat aattcatcac accaatacgt gatcgcaatt
     8641 cttcattcca gtcaatcatt gataataatg ttgcgcgggt ttgtttggct ggtagatgat
     8701 atcccgtaca atttccttct gatattaatt tgtatggtat atcgtttttc attaaaccat
     8761 cagccagccc cataatataa ggagcctggg taataccgat aagtacgttt tcttctctac
     8821 gcagagagat aactccttct aaaataacga aagtatcttc gttattgaca tcactggaaa
     8881 atataacttg cttttcatta ttaaactcga agcgcgtacc gtatctggac aaacatttat
     8941 cgagcttacc aaattcctga agaggtttaa ctacagataa catttgcgcg tcctttgcag
     9001 taatgcccgt caaatccttg acgggcatta tttagattaa attaccagta tttcttcgga
     9061 gtgaagaata ttaccaagta tatttaacac ccacgttcgc ggaccagtct tgatctacgt
     9121 caccaccacc gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact
     9181 gagtacccag accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt
     9241 tcacatcgtt atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca
     9301 gagcctgatc ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca
     9361 tgctgtcgta aggctgaccg tcaactttca tgtcgttgct cagctggtag tcatcaccag
     9421 actggaacag accagaaatg ctgccgtaag gagtcacgta accagcatca cccagtttga
     9481 agtcgtaacc ggttttcaaa ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt
     9541 aagtaccgtt gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagc
//
LOCUS       DADLCQ010000143         9554 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000143 DADLCQ010000000
VERSION     DADLCQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.145"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="JQG95_003458"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="JQG95_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY5889898.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="JQG95_003459"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="JQG95_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY5889899.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREILALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="JQG95_003460"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="JQG95_003460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY5889900.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="JQG95_003461"
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="JQG95_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY5889901.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="JQG95_003462"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="JQG95_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY5889902.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="JQG95_003463"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="JQG95_003463"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY5889903.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /locus_tag="JQG95_003464"
     CDS             complement(6042..7181)
                     /locus_tag="JQG95_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY5889904.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7840..9423
                     /locus_tag="JQG95_003465"
     CDS             7840..9423
                     /locus_tag="JQG95_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY5889905.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         2222 a         2460 c         2440 g         2432 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagtat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctggca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgagtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggc
//
LOCUS       DADLCQ010000144         9493 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000144 DADLCQ010000000
VERSION     DADLCQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.146"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..190)
                     /locus_tag="JQG95_003466"
     CDS             complement(<1..190)
                     /locus_tag="JQG95_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889906.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="JQG95_003467"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="JQG95_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY5889907.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="JQG95_003468"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="JQG95_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY5889908.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="JQG95_003469"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="JQG95_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY5889909.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="JQG95_003470"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="JQG95_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="JQG95_003471"
     CDS             complement(3262..3381)
                     /locus_tag="JQG95_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY5889910.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /locus_tag="JQG95_003472"
     CDS             complement(3342..3527)
                     /locus_tag="JQG95_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889911.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /locus_tag="JQG95_003473"
     CDS             3628..3801
                     /locus_tag="JQG95_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889912.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3863..4147
                     /locus_tag="JQG95_003474"
     CDS             3863..4147
                     /locus_tag="JQG95_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY5889913.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="JQG95_003475"
     CDS             4151..4486
                     /locus_tag="JQG95_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY5889914.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="JQG95_003476"
     CDS             complement(4543..6864)
                     /locus_tag="JQG95_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY5889915.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVYNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="JQG95_003477"
     CDS             complement(6981..7190)
                     /locus_tag="JQG95_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889916.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7609..7845)
                     /locus_tag="JQG95_003478"
     CDS             complement(7609..7845)
                     /locus_tag="JQG95_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889917.1"
                     /translation="MFESFDRIAISSMNNSNNLDYFTLYIIFSIAFMLITLLVILIAK
                     PSTGLGEVLVTINLLNALVWLAINLVNRFKRKTR"
     gene            complement(7941..9464)
                     /locus_tag="JQG95_003479"
     CDS             complement(7941..9464)
                     /locus_tag="JQG95_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY5889918.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVMVE"
BASE COUNT         2659 a         2203 c         2080 g         2551 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ttggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatggcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttggtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattataaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatt
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc ataaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgcagg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcacccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa tac
//
LOCUS       DADLCQ010000145         9271 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000145 DADLCQ010000000
VERSION     DADLCQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.147"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            12..278
                     /gene="mliC"
                     /locus_tag="JQG95_003480"
                     /pseudo
     CDS             12..278
                     /gene="mliC"
                     /locus_tag="JQG95_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
     gene            337..993
                     /gene="pdxH"
                     /locus_tag="JQG95_003481"
     CDS             337..993
                     /gene="pdxH"
                     /locus_tag="JQG95_003481"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY5889919.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1122..2396
                     /gene="tyrS"
                     /locus_tag="JQG95_003482"
     CDS             1122..2396
                     /gene="tyrS"
                     /locus_tag="JQG95_003482"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY5889920.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2458..3318
                     /gene="pdxY"
                     /locus_tag="JQG95_003483"
     CDS             2458..3318
                     /gene="pdxY"
                     /locus_tag="JQG95_003483"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY5889921.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="JQG95_003484"
     CDS             complement(3362..3967)
                     /gene="gstA"
                     /locus_tag="JQG95_003484"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY5889922.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="JQG95_003485"
     CDS             complement(4073..5575)
                     /gene="dtpA"
                     /locus_tag="JQG95_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY5889923.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6186..6821)
                     /gene="nth"
                     /locus_tag="JQG95_003486"
     CDS             complement(6186..6821)
                     /gene="nth"
                     /locus_tag="JQG95_003486"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY5889924.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6821..7516)
                     /locus_tag="JQG95_003487"
     CDS             complement(6821..7516)
                     /locus_tag="JQG95_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY5889925.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="JQG95_003488"
     CDS             complement(7520..8140)
                     /gene="rsxG"
                     /locus_tag="JQG95_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY5889926.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="JQG95_003489"
     CDS             complement(8144..9202)
                     /gene="rsxD"
                     /locus_tag="JQG95_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY5889927.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2391 a         2317 c         2349 g         2214 t
ORIGIN      
        1 aaatataggg gcaaatccag gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg
       61 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
      121 ataatcaact actgcatctc aaacagggcc tttcagcctc tggcgcgcgt tactccgacg
      181 ggatctatgt tttctggtcg aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg
      241 tcttgaataa ctgtcagtta caaaatccac agcgttgaga tttttccagg ggcggcgcac
      301 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
      361 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatcttccc
      421 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggcg
      481 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc
      541 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggtagccgt
      601 aaagcacatc aaattgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
      661 gaacgccagg tgatggtgat cggtaaagca gaacgacttt cgactctcga agtgatgaaa
      721 tattttcata gccgcccgcg tgatagccag attggtgcat gggtttcgaa gcagtccagt
      781 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
      841 cagggcgaag tgccattgcc gagcttttgg ggcggttttc gcgtcagcct tgaacagatt
      901 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
      961 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
     1021 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
     1081 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
     1141 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
     1201 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
     1261 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
     1321 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
     1381 ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc
     1441 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
     1501 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
     1561 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
     1621 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
     1681 acaaacagta cgatgtggtg ctgcaaattg gtggttctga ccagtggggt aacatcactt
     1741 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcctg accgttccgc
     1801 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
     1861 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
     1921 acgtttaccg cttcctgaag ttcttcacct ttatgagcat tgaagagatc aacgccctag
     1981 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg
     2041 tgactcgtct ggttcacggt gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc
     2101 tgttcagcgg ttctttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
     2161 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
     2221 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
     2281 acggtgaaaa acagtccgat cctgaatact tctttaaaga agaagatcgt ctgtttggtc
     2341 gttttacctt actgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
     2401 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
     2461 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
     2521 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
     2581 aatcacaccc aatacggcaa atggactggc tgcgtgatgc cgcccagcca tttaaccgaa
     2641 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
     2701 tatctgggat cggcggagca gggtgaacat atcctcggta tcgtccgtca ggtgaaagca
     2761 gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
     2821 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
     2881 attgcgccga atctggttga gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa
     2941 gaagcggttc tggcagcgcg cgaactcatt gagcaagggc cacaaattgt gttggttaaa
     3001 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctggt caccgccgat
     3061 gaagtctggc atatcagccg tccactggtg gattttggta tgcgccagcc ggtaggtgtt
     3121 ggtgatgtga cgagcggttt actgctggtg aaactgcttc agggggcaac gctgcaggag
     3181 gccctggaac atgtgaccgc tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag
     3241 gaatatgagc tgcaagtggt ggctgctcag gatcgtattg ccaaaccaga acattacttc
     3301 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
     3361 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
     3421 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
     3481 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct
     3541 catccttcag tgcctcgttc acatattgca gcttcttatc cagctgcgcg cgaactgtcg
     3601 gtttgtactc ttccggtgta tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg
     3661 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
     3721 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca
     3781 gcaaagtacc atcgtccagc agcaatgcag gcacctgccc cttagggtta acggaaaagt
     3841 aatcgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
     3901 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
     3961 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
     4021 aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag tttccctgaa ttttacgcta
     4081 cagtagcttt cgccgctttg tctgcagcgt catcctgcgt catgcggtgc agtttaggcg
     4141 cggtcagcag catcagtact gcaataacgg cagtagcgac accaatctgc aagaatactc
     4201 gaccatagac ttccagtgac atcagcgggt cggtaacgtt atccggcaca gccatcatac
     4261 ccgcaacata accaccaatc aggtttgcac cggcagtggt caggaaccag ctacccataa
     4321 tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat tgccagaccc agaccagaga
     4381 tcatcagttc cccgatgctc tgcaggccat agcttgcgac cagccagctg acagacacga
     4441 tacctgcgtc agacgcgaat ttcgcaccca gcggcagaat caggaacgca ccagaacaca
     4501 tcaccatgcc gattgcaaac ttggttggca tcggcagggt atcacccatc ttgttataga
     4561 tagcggccag aatcggacta ccgatgatga tccagaacgg gttcagtgcc tgatactgct
     4621 caggttctac ggccagaccc agaatggagt gctcaacgtt acgaatcgca aagaagttca
     4681 gtgacgttgg catctggctg tacagcacga agaagatgat tgcttcgagc atcaggatga
     4741 aggcaacgat cattttacga cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga
     4801 taaccacaat accgaaggca acaacgccca gcgccatacg cgcaacttcc tgattgtgca
     4861 gtagccaggt ggcaacagcg atcagcgcca caacaccaat aatggtcagc aacaggttac
     4921 ggtagttgac tggttcaaag tctggttttg aaccgtactg tttaacccag cgttggcaga
     4981 aggcgaagtt aacgatagtg atcagcaggc ctacaacgct caacgcaaac gcaacactcc
     5041 agccgtattt cgcggccagc cacggggtgg ctatcataga gaagaaagag ccgatgttga
     5101 cggacatgta gtacatggtg aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg
     5161 tagaaagcag agaagacggg ttagctttaa acaggccgtt accgaccgca atagccgcca
     5221 tacccatata aacaataccg gcgtcgtgac cagaccaggc aaccagagca taaccaatcg
     5281 ccagcacaat agcgccgagc ataattacgc gtttagtacc cagtaccttg tcacctaacc
     5341 agccgccgat agcgaccaga ccataaacca gggcactaaa ggaagagaaa agggtgattg
     5401 aatccgcttc agacataccc agttgtttaa ccaggtaaac agccataatt ccttgtaggc
     5461 cgtaataacc aaaacgttcc cataactcaa tcgagaagat gagatagaac gccttcggtt
     5521 gtttgaaagc gttcaaactg acgctttcag ttggtttttg gtttgcagtg gacactttta
     5581 cctctttttt tacatcccat attaacgggg gtgctttctc gcgttatgtc taaaaaacat
     5641 acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat gttcactatc ttgcactttc
     5701 agacaagtcc tttgtaataa tctgttacat ataactgagg cggggaattc aaccattccg
     5761 ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac ggcagatttt ttcactgcat
     5821 tgagaggggg atgatggctt ggcggcgata tgttctgcta tttagcgttt ttcctagtga
     5881 gatagtccag tttctgaaaa atagccagtg taatgttttg caggtcaata tttgttgcgt
     5941 gtgtaaattt aaatcatttt aacctgattc ttgcaatgtt tttacattgc cacagcatcg
     6001 tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta acctgcattt
     6061 ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc attgacgtta
     6121 aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg tgttacccct
     6181 tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat acaagagcca
     6241 cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca atggtggcag
     6301 tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg ttcgacgttt
     6361 ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata
     6421 gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc tacgccgggc
     6481 agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgttgttc cagtaatata
     6541 cggcaggttt tgatgatatt ttctgctttg ctgttataaa ggccaatcgt tttgatatag
     6601 gttttcaccc cttcaacgcc tagttcaagc atcgctgcag gcgtattcgc caccgggtag
     6661 agtttcgccg tcgccttatt aacactgata tcggtcgcct gagcggaaag cagtacggca
     6721 atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg attgttctca
     6781 cgcaggcgag tgaggatctc cagacgtttt gctttgttca tcagacattc cctgtttcac
     6841 cgtttggcaa tgcacgttcg gcagctgctt cagcacggcg ctttttcatt ctttcatcaa
     6901 tcaggtattt tcctgccagc atcagtccca agccaataaa tgcacctggt ggcagcatcg
     6961 ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt gcccagctac
     7021 ctaacagcgc atctgcaccg tcaaacaacg tgccattgcc gataatttcg cgtagtgaac
     7081 ccagcacgaa catggcgcag gttgcgcccg taccaattga aaagccgtcc agtgccgaaa
     7141 gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata cagttagtga
     7201 caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag gcgttgatca
     7261 gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga atgcggatct
     7321 cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg
     7381 tagtcgcaag tcctaaaccc agagcgttag tggcagtgga tgtgaccgcc aatagcggac
     7441 aaaggccgag cagttggacc agtgcagagt tgtttttcca caacccctga acaataacgt
     7501 ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt tgtgccggta
     7561 acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg
     7621 tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag tgcgcatcat
     7681 ccgcaccact gatttttttt cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga
     7741 ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc
     7801 cattaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct ggcgcggttg
     7861 cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac
     7921 ctttacctaa ctctggcgca gttaccagat agcaactctg tgcgagcgca ttgttatagc
     7981 gttcggctgg cagcacctga tcaaataacg tcttttgttg cagactggcc tgttcagcaa
     8041 ttgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgtagaaccc gctgcaaata
     8101 gtgccagtgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct ttgcgatggc
     8161 cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc gccagcagga
     8221 cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag actaataagc
     8281 ccgccagcgc gccgaacatc agccgaccac gattggtcgt agaagcagta actgggtcag
     8341 tcaaaataaa gaatgcgccg agcatagtcg caccagacag cagatgaatt tgcggtgctg
     8401 ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc gttactaaga
     8461 agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacacg ccaccagcca
     8521 gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg tagatcggat
     8581 attgcataat ctgttcaacc gaatgacctg cgcggacaga ggttttgaat gtatccagcg
     8641 gtgtcgcctg actaatgcca tcaataccta agcgtagcgt attcatatca ccaccactgg
     8701 cggtatgacc gctgaaaata acctgtatgg cgtcgataaa accagggatg ttgaccgcaa
     8761 tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt aagaccacat
     8821 aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac tgtttagcga
     8881 tgatcaccgc aaacaccgta cccagtacga ccatccacca tggcgctagg gggggaatac
     8941 ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg
     9001 actgcttgcg tagtttgagt acgagagctt cggctaacag agcactgacc gacgccaaca
     9061 ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt cctggcacgg
     9121 ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg gtataagggg
     9181 agctagctat tctgaatacc atttagtcct cgtttacaac cttttgctgg gcggcttttt
     9241 tggcctgaac gcgggcaata gccgcggcaa c
//
LOCUS       DADLCQ010000146         9254 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000146 DADLCQ010000000
VERSION     DADLCQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.148"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JQG95_003490"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JQG95_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY5889928.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLA
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JQG95_003491"
     CDS             1828..3147
                     /locus_tag="JQG95_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY5889929.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLHDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4669
                     /locus_tag="JQG95_003492"
     CDS             3524..4669
                     /locus_tag="JQG95_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY5889930.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JQG95_003493"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JQG95_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="JQG95_003494"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="JQG95_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="JQG95_003495"
     CDS             5815..6711
                     /locus_tag="JQG95_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY5889931.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7694
                     /locus_tag="JQG95_003496"
     CDS             6708..7694
                     /locus_tag="JQG95_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY5889932.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7687..8613
                     /locus_tag="JQG95_003497"
                     /pseudo
     CDS             7687..8613
                     /locus_tag="JQG95_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="JQG95_003498"
     CDS             complement(8669..9100)
                     /gene="osmC"
                     /locus_tag="JQG95_003498"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY5889933.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTTPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2262 a         2262 c         2320 g         2410 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggc tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaca tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 aaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctgccgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctaggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgccctgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gctggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgaa aacgtcccgg
     7621 cgctgacagc gtgtggtgac aacaaccagc actgcgcctg ttggtatccg cagcaggagg
     7681 tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc atatcaattt tccggcccgt
     7741 aaaaactggc ttggtaaatc tacggaacat gttcatgcca ttaatggtat tgatttacag
     7801 atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag gctgcggcaa aagcaccctc
     7861 gcacagcttt taatgggcat gctgcaaccg agccacgggc agtacatccg ttcaggctca
     7921 caacgcatta tgcagatggt gtttcaggac ccgctctctt cgcttaatcc gcgcttaccg
     7981 gtgtggcgca tcatcacaga atcgctctgg atagctaagc gtagtagtga acaacagcgg
     8041 cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc gtccggagta tctcgaccgc
     8101 ctgcctcatg cgttctccgg cgggcagcgg caacgcatcg ccattgccag agcactctct
     8161 tcgcagcctg acgtgattgt gcttgatgag ccaacctcgg cgctggatat ctccgtgcag
     8221 gcgcagatcc tcaatttgct ggtaacgcta caggaaaatc acgggctgac ttatgtgctg
     8281 atttcacaca atgtctcggt gatacgtcat atgagcgatc gggtggcggt gatgtatctc
     8341 gggcagattg tagaactggg cgacgcgcaa caggtgttga ctgcttcggc acatccatac
     8401 acccgattat tgctggattc cctccccgcc attgataaac cgctggagga agaatgagca
     8461 ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc aaggctgttt tttctacgaa
     8521 cgttgccctc tggcaaccca cggatgtgaa gtccggcaat cattagcaat aagggaggac
     8581 ggacgtgagc tccggtgctg gcgggcgctg tagagatgct aacaacgcat caggcattca
     8641 cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa ctggtaatcc agcgtaattt
     8701 ccgctttcag tacctgagag accgggcatc ctgcttttgc tttctggatg atgccgtcaa
     8761 aggtagaggc atcaataccc ggcaccgcaa cttcactctt cagtgcgatt ttcgtaatcg
     8821 caaaaccggc atccacttta tccagcgaca catcggcggt ggtatcaatt gatgttggcg
     8881 tgaatcccgc ttcccccagc attaatgaaa gcgccattga gaaacatgcg gcatgcgctg
     8941 cgccaatcag ttcttcaggg gtggttcctt tttcgccttc aaaacgcgtg ttaaatccat
     9001 acggctgttg gttcagcacg ccgctctcgg tggatactgt tcccttcccg cgtttgatat
     9061 cgccttccca gtgtgcctga cctttcttat ggattgtcat tgttgctctc ctgtgggctt
     9121 aaaaaggaaa ttatagacta cgtgagaaac agcacgagga taagcaggat attccgaata
     9181 aaatacggcg aaaatattac ttctgcactt aaattaaatt cccacaatag ataaccggga
     9241 atatatatct caag
//
LOCUS       DADLCQ010000147         9156 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000147 DADLCQ010000000
VERSION     DADLCQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.149"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..493)
                     /locus_tag="JQG95_003499"
     CDS             complement(<1..493)
                     /locus_tag="JQG95_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5889934.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JQG95_003500"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="JQG95_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY5889935.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="JQG95_003501"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="JQG95_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY5889936.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="JQG95_003502"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="JQG95_003502"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY5889937.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="JQG95_003503"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="JQG95_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY5889938.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="JQG95_003504"
     CDS             3647..4156
                     /locus_tag="JQG95_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY5889939.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="JQG95_003505"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="JQG95_003505"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY5889940.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="JQG95_003506"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="JQG95_003506"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY5889941.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="JQG95_003507"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="JQG95_003507"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY5889942.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="JQG95_003508"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="JQG95_003508"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY5889943.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="JQG95_003509"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="JQG95_003509"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY5889944.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYAYGVIWAQSKFS"
     gene            complement(8798..>9156)
                     /locus_tag="JQG95_003510"
     CDS             complement(8798..>9156)
                     /locus_tag="JQG95_003510"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY5889945.1"
                     /translation="ICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGLVILTD
                     ADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAWIGALS
                     RIEETLAGEAGTCISL"
BASE COUNT         2207 a         2355 c         2313 g         2281 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatgcaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 acacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catcgggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgcc
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgtta attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgcctacgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggagc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatca
//
LOCUS       DADLCQ010000148         9022 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000148 DADLCQ010000000
VERSION     DADLCQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.150"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..923
                     /locus_tag="JQG95_003511"
     CDS             <1..923
                     /locus_tag="JQG95_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5889946.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JQG95_003512"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JQG95_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY5889947.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWENRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQG95_003513"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JQG95_003513"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY5889948.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JQG95_003514"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JQG95_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY5889949.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JQG95_003515"
     CDS             4446..4805
                     /locus_tag="JQG95_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY5889950.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="JQG95_003516"
     CDS             complement(4845..5933)
                     /gene="trmA"
                     /locus_tag="JQG95_003516"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY5889951.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRAQNLNVHLIGRATKTKIALD
                     QDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELYCGNG
                     NFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNGVREF
                     NRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLETLSQ
                     THKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6047..6237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6302..8146
                     /gene="btuB"
                     /locus_tag="JQG95_003517"
     CDS             6302..8146
                     /gene="btuB"
                     /locus_tag="JQG95_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY5889952.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8091..8948
                     /gene="murI"
                     /locus_tag="JQG95_003518"
     CDS             8091..8948
                     /gene="murI"
                     /locus_tag="JQG95_003518"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY5889953.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2054 a         2179 c         2435 g         2354 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcacc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg agaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag ggaaaccacc
     5581 gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg cagaacggga
     5641 ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc actggcggcg
     5701 gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat gtgatacagg
     5761 tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact gaccggcgag
     5821 cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa acgtaccact
     5881 ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt catgatgtat
     5941 cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc cagatcttga
     6001 tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt agcatccact
     6061 tgccggtcct gtgagttaat agggaatcca gtgcgaatct agagctgacg cgcagcggta
     6121 aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag tcatcatctc
     6181 ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg catctggttc
     6241 tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg gatgctttac
     6301 aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt ccgcttgggc
     6361 acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac agccgcgcag
     6421 cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac
     6481 ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa acggcggttc
     6541 aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc tggtgttaat
     6601 tgatggcgtc cgcctgaatc tggcgggggt gagtggttct gccgacctta gccagttccc
     6661 tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg tttatggttc
     6721 cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg gaacggaaat
     6781 ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc agcaacaact
     6841 gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg gttatgatgt
     6901 tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt ttttaagtaa
     6961 aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct ttgttcgcgg
     7021 ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt caccgttgct
     7081 cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata acggcgaact
     7141 gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact acgatcccca
     7201 ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca ccgtccagtg
     7261 ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca
     7321 gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta ataccggcat
     7381 ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag cacgcagcga
     7441 cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt gggaattcat
     7501 cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa atctggggca
     7561 actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac agtgggaagg
     7621 cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt
     7681 cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag ggaaagcgcg
     7741 gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacgc atactgtgag
     7801 ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac gccgtgctaa
     7861 acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg gtattactta
     7921 tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc aaaccgttaa
     7981 aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct ctcacctgac
     8041 agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     8101 aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     8161 tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     8221 taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     8281 gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     8341 cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt cctgcattac
     8401 gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     8461 tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     8521 ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     8581 agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg gatgcactaa
     8641 aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     8701 gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     8761 tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     8821 cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccagaagctg
     8881 aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     8941 taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag
     9001 gccggaataa ctccctataa tg
//
LOCUS       DADLCQ010000149         9014 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000149 DADLCQ010000000
VERSION     DADLCQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.151"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQG95_003519"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="JQG95_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY5889954.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQG95_003520"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="JQG95_003520"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY5889955.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQG95_003521"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="JQG95_003521"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY5889956.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQG95_003522"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="JQG95_003522"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY5889957.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="JQG95_003523"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="JQG95_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY5889958.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="JQG95_003524"
     CDS             5545..6018
                     /locus_tag="JQG95_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY5889959.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="JQG95_003525"
     CDS             6047..6589
                     /locus_tag="JQG95_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY5889960.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="JQG95_003526"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="JQG95_003526"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY5889961.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYDKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="JQG95_003527"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="JQG95_003527"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY5889962.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNVFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="JQG95_003528"
     CDS             7986..8243
                     /locus_tag="JQG95_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY5889963.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="JQG95_003529"
     CDS             complement(8219..8773)
                     /locus_tag="JQG95_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY5889964.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2257 a         2187 c         2247 g         2323 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 atggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 tgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatggcaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatgata aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgttttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag agaa
//
LOCUS       DADLCQ010000150         8989 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000150 DADLCQ010000000
VERSION     DADLCQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.152"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            146..2330
                     /locus_tag="JQG95_003530"
                     /pseudo
     CDS             146..2330
                     /locus_tag="JQG95_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="JQG95_003531"
                     /pseudo
     CDS             complement(2299..4036)
                     /gene="aceK"
                     /locus_tag="JQG95_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="JQG95_003532"
                     /pseudo
     CDS             complement(4220..5523)
                     /gene="aceA"
                     /locus_tag="JQG95_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="JQG95_003533"
                     /pseudo
     CDS             complement(5553..7154)
                     /gene="aceB"
                     /locus_tag="JQG95_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7423..8352)
                     /gene="metA"
                     /locus_tag="JQG95_003534"
     CDS             complement(7423..8352)
                     /gene="metA"
                     /locus_tag="JQG95_003534"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY5889965.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8509..8952
                     /locus_tag="JQG95_003535"
     CDS             8509..8952
                     /locus_tag="JQG95_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY5889966.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2356 a         2168 c         2167 g         2298 t
ORIGIN      
        1 cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag cgatttatga
       61 tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta aaattcaaga
      121 catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt ctttctctgc
      181 aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat tgttttataa
      241 agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa aaagcttctc
      301 agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg tcattaaatt
      361 ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata aaaacgaaaa
      421 cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg aaattttacc
      481 tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata gaaaagaaaa
      541 agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc ccaaaaccgt
      601 atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
      661 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      721 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg cgttgctgcc
      781 tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc aaatggcatc
      841 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      901 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtaact
      961 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
     1021 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt tattaaccaa
     1081 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggaactgtg
     1141 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
     1201 aatcaccaac tcacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
     1261 gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc tataaattat
     1321 ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg atatgaaggg
     1381 ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa tggtttttct
     1441 ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat tctgaacgtc
     1501 ttacctaaac tggccacaac acatcattta gataatgaac aggtgtataa attcctgagt
     1561 gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg tgatgccgac
     1621 atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca tttgactaaa
     1681 gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag actatacctg
     1741 gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt gccctgtctg
     1801 gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc taaaagtctt
     1861 gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt tattaatact
     1921 atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa aatatgaagc
     1981 ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg atacagcata
     2041 aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca cgcctgtttg
     2101 gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa aaatactctg
     2161 cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc cttaatacat
     2221 taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt gcggagaaaa
     2281 attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa atctttgcct
     2341 gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt gtagtgcgcg
     2401 ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac caatacgcgg
     2461 gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg agacgctgta
     2521 ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt cgcggaaatt
     2581 cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc cgtgacgggt
     2641 gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg caagctggcg
     2701 aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca cttgttccag
     2761 ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt acaatctgct
     2821 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aataccgggg
     2881 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     2941 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     3001 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     3061 acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     3121 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     3181 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     3241 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     3301 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     3361 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     3421 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     3481 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     3541 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     3601 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     3661 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     3721 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     3781 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     3841 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     3901 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     3961 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     4021 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     4081 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     4141 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacagtc
     4201 agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc agcgcggtga
     4261 ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct gtacccactt
     4321 cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc ggctgctgca
     4381 ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc aggtcgaaca
     4441 tgttaaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg tagcccatat
     4501 ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc tgccagttga
     4561 acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg atagcttgtg
     4621 caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc aggtcagcat
     4681 atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga gtacggaaga
     4741 agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg caatctgagg
     4801 tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga acgcccgtca
     4861 cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt ggcactaaaa
     4921 ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg aagtgaactg
     4981 ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg acaccgccaa
     5041 aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc ggatcgcccg
     5101 gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg ttgatccgct
     5161 ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac atgctggccg
     5221 ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca atacccgctt
     5281 tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg tagctttttt
     5341 cgactcaccg tgcagcagac gccacatttt cgctgcgccc agttgcgcca gcgtgcattc
     5401 aggattgact gaaccgcgta atttcaccac atcttccgca ctgtatgggc gagtaatgcc
     5461 ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg tacgggtttt
     5521 catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta gcctggcagg
     5581 gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg tgcggcatcg
     5641 tcaaaacggc cctgggagaa acgttcttcg cccagttcgc tggcaatgac tttcatctct
     5701 tcgcccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct caacgttttt
     5761 tgatgatgga tccactgcca gatcgaggta cgggaaattt cagccgtcgc cgcatcttcc
     5821 atcaggccat aaatcggcac gcagccgttg ccggagatcc acgcttcgat gtactgcaca
     5881 gccacgcgaa tgttagcgcg cataccttct tcggtgcgtt caccatcaca aggtgccagc
     5941 agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcaata cttcaagctg atttttacgg
     6001 gagccgagaa tgtcgttgaa taccgccatt gccgtgtccg caaggcctgg gtgagcgatc
     6061 catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac tttgttgagc
     6121 acctggttat tacgctcttc atctttgctc ggaataaacg cggccatgcc gcccatcgca
     6181 aaagcaccgc gtttatggca ggttttaatc agcaggcgtg agtaagcatt caggaatggt
     6241 ttatccatcg tcacggcctg tctgtctggc aggacacgat cgggatagtt tttcaacgtt
     6301 ttgatatagc tgaagatgta atcccagcga ccgcagttca gaccaacaat atggtcgcgc
     6361 agcgcgtgaa ggatttcgtc catctggaac acggcgggca gcgtttcaat cagcaacgtc
     6421 gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa gacttcgctc
     6481 caccaggccg cttcctgcta ggactgggtt ttcggcagat agaaataggg accgctgccc
     6541 tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa caggctaccg
     6601 gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc gcgtacccga
     6661 caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc attggtatag
     6721 ctgatggtac cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac tttgttccag
     6781 tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc gttcagtgcg
     6841 ttgatcacca tcttgcgctc taccgggcca gttatctcta cgcggcggtc ttctaagtcc
     6901 gcaggaatcc cgcgaatttt ccaatcagca tcgcgaatgg aagccgtttc cgaaataaaa
     6961 tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc cagaagttta
     7021 ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc ttcggcagta
     7081 agaatttgct tctcctgctc gccatacggc cttgtgaaag ccagttcatc ggttgttgtt
     7141 gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc tctgtgaacg
     7201 aaggaacgtt gtgcgctttt cgttcagcac aactaagact actcatttaa aatgcaaaat
     7261 caaaaacaat ttccatttta attaaaaata cttgataact tattgataac aatatgaata
     7321 aaatttattc acttgctgag gtgcgtttcg gaaataaaaa aggcacccga aggtgcctga
     7381 ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca gcgttggatt
     7441 catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc agttggtaaa
     7501 cagcaaatta ccgtgactac gccagctcgc tcgcggtttg ttttgcggat cgttgtgcgg
     7561 gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgta aatattcctg
     7621 cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc gcttatcttt
     7681 gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca gatcggtgta
     7741 atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgcggtgcca ggaatgaatc
     7801 atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt aaacgccaga
     7861 gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg cggcctgtac
     7921 cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca gcacctgttt
     7981 gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg caccggttac
     8041 aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga agttgttcag
     8101 atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct gaatatcgac
     8161 ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa tcttcttcgg
     8221 catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg cacgagaagt
     8281 tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct cgtccggcac
     8341 acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga catccagata
     8401 gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt gagaaagttt
     8461 caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat ggttattagt
     8521 attcgccgct cacggcatga ggaaggggag gaactcgttg cgatttggtg tcgatctgtc
     8581 gatgccaccc acgatttttt atcagcagag tatcgggccg agctggaaga gctggtgcgt
     8641 tcctttctgc cggaagcacc gttgtgggtc gcggttaatg aacgggagca gccggttggg
     8701 tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga tgtgtgcggc
     8761 tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga actgacaacc
     8821 aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg ttttaaggtt
     8881 acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cgttgctgaa tctggcgtat
     8941 gtgggggaat aagccgcccc tgccgtgcct gcccaagctg gagaagacg
//
LOCUS       DADLCQ010000151         8895 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000151 DADLCQ010000000
VERSION     DADLCQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.153"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            369..2887
                     /locus_tag="JQG95_003536"
                     /pseudo
     CDS             369..2887
                     /locus_tag="JQG95_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JQG95_003537"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JQG95_003537"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY5889967.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="JQG95_003538"
                     /pseudo
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="JQG95_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(5343..5564)
                     /locus_tag="JQG95_003539"
     CDS             complement(5343..5564)
                     /locus_tag="JQG95_003539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY5889968.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5760..7283)
                     /locus_tag="JQG95_003540"
     CDS             complement(5760..7283)
                     /locus_tag="JQG95_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY5889969.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEKKLALAALHDTARERQK"
     gene            complement(7280..8041)
                     /locus_tag="JQG95_003541"
     CDS             complement(7280..8041)
                     /locus_tag="JQG95_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY5889970.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8038..8697)
                     /locus_tag="JQG95_003542"
     CDS             complement(8038..8697)
                     /locus_tag="JQG95_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY5889971.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPIDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2174 a         2357 c         2319 g         2045 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat aagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtagtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc aggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccaacaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt atcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtagaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gttcccttgc agtgtcatga agtgctgcca gagcgagttt cttttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat atccgtggcg aggatatcat
     7801 taagccccaa ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg
     7861 cgagcatcgc gccttcgccc ccctgcgaaa cgctacctgc gccatcagca aggaacatca
     7921 ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca tcctggcaag
     7981 gcaagtccgc gctgatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca
     8041 cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga
     8101 aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag
     8161 aggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc cctgaacacc
     8221 gatggaaaag aaagcaaacc ttttatcttc ttccccccga aacactttgt tggctgcagc
     8281 ctgccactca tcgattggtg caccatcggt aatcaggaaa atccacgggc gataatacga
     8341 aataccattt gcacgatact cacgtttccg ctcctctacc atatccaggg ctttggtaat
     8401 ggccgcaccc attggtgtat cgccctgggc aaacaggatg ggcggaaaaa aattagcggc
     8461 gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga gttccactct
     8521 ttttaaagcc agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggtgttaag
     8581 ttcattaata ggtctgccac tcatcgagcc agaaacatcc agcagcagaa tgcaggggca
     8641 acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag
     8701 aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa
     8761 gagcaaacga agaaactttc agaattagtc ccggcggcgc agaaaatttt gctattcgcc
     8821 tcgcaagaac ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg
     8881 agggttaggg agagg
//
LOCUS       DADLCQ010000152         8646 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000152 DADLCQ010000000
VERSION     DADLCQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.154"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JQG95_003543"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JQG95_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY5889972.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3184)
                     /gene="hemX"
                     /locus_tag="JQG95_003544"
     CDS             complement(1967..3184)
                     /gene="hemX"
                     /locus_tag="JQG95_003544"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY5889973.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(3206..3946)
                     /gene="hemD"
                     /locus_tag="JQG95_003545"
     CDS             complement(3206..3946)
                     /gene="hemD"
                     /locus_tag="JQG95_003545"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY5889974.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3943..4884)
                     /gene="hemC"
                     /locus_tag="JQG95_003546"
     CDS             complement(3943..4884)
                     /gene="hemC"
                     /locus_tag="JQG95_003546"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY5889975.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5271..7817
                     /gene="cyaA"
                     /locus_tag="JQG95_003547"
     CDS             5271..7817
                     /gene="cyaA"
                     /locus_tag="JQG95_003547"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY5889976.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7857..8177)
                     /gene="cyaY"
                     /locus_tag="JQG95_003548"
     CDS             complement(7857..8177)
                     /gene="cyaY"
                     /locus_tag="JQG95_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY5889977.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8225..>8646)
                     /locus_tag="JQG95_003549"
     CDS             complement(8225..>8646)
                     /locus_tag="JQG95_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY5889978.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1967 a         2235 c         2225 g         2219 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatccacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac
     2101 gcacgcgagt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     2161 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     2221 catcatcagt atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat
     2281 aagtctcttc ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     2341 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     2401 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     2461 attcgctgat ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt
     2521 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     2581 tgccgtcata atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc
     2641 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca
     2701 gcaacgctgc agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     2761 ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     2821 tcttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct
     2881 taagctgcgc agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg
     2941 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     3001 cctgttgttt accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca
     3061 ccgcgctgag aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg
     3121 acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg
     3181 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag
     3241 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     3301 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     3361 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg
     3421 attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     3481 cacaaaaagt gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc
     3541 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     3601 gcaagacttc gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat
     3661 gtagcgccag tgcggtggtg cgtccaatgg cgaaataagt aggtagttgg ggccatttac
     3721 gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     3781 acagatcgct ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg
     3841 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca
     3901 ctaactcttc tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc
     3961 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     4021 aatccccatt tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     4081 accgtccggc gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta
     4141 gctaccaatt ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt
     4201 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc
     4261 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     4321 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     4381 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     4441 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     4501 ggacgtcccg acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac
     4561 aaaggcatcg cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa
     4621 ttcaaccggc acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc
     4681 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     4741 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc
     4801 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     4861 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     4921 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     4981 aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac
     5041 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     5101 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     5161 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     5221 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     5281 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     5341 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     5401 atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     5461 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     5521 cagtacagga tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     5581 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     5641 ggctcgatag cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg
     5701 ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata
     5761 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     5821 acgaatttta tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     5881 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     5941 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     6001 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga
     6061 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     6121 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca
     6181 tgatgctgga gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt
     6241 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg
     6301 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     6361 gtctggcaat gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca
     6421 acgagctgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     6481 ataaccttag cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg
     6541 ccgcgtttga agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     6601 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt
     6661 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     6721 ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc
     6781 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     6841 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     6901 acagcccgtg tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag
     6961 ctgcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     7021 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     7081 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     7141 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     7201 agcatctgcg cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     7261 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     7321 aaacctgggg cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca
     7381 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa
     7441 ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt
     7501 tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca
     7561 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca
     7621 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     7681 acctgccgca gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc
     7741 gaacaaaatc gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac
     7801 agcagtattt ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag
     7861 cggaaactga ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag
     7921 gtttcgccgc tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc
     7981 tgtttggttg ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg
     8041 ccgttttcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat atcgctgtcg
     8101 ccatccctgt cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggcgatga
     8161 aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat
     8221 ggggtcaggg cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca
     8281 ccggaataat gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt
     8341 tcgccgggca gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc
     8401 gatcgtcata agcgaagcga tcgtttttag ttagcgaacc gttaactgga ataatttgca
     8461 agctctttga ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc
     8521 gatcaatgac caggtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg
     8581 tcgataattt tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat
     8641 ggaatt
//
LOCUS       DADLCQ010000153         8475 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000153 DADLCQ010000000
VERSION     DADLCQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.155"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            44..397
                     /locus_tag="JQG95_003550"
     CDS             44..397
                     /locus_tag="JQG95_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY5889979.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQG95_003551"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="JQG95_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY5889980.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQG95_003552"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="JQG95_003552"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY5889981.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQG95_003553"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="JQG95_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY5889982.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQG95_003554"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="JQG95_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY5889983.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQG95_003555"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="JQG95_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY5889984.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQG95_003556"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="JQG95_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY5889985.1"
                     /translation="MHEITLCQRALELIEQQAAKHDAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="JQG95_003557"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="JQG95_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY5889986.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLYH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /locus_tag="JQG95_003558"
     CDS             7001..7612
                     /locus_tag="JQG95_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY5889987.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7649..>8475
                     /locus_tag="JQG95_003559"
     CDS             7649..>8475
                     /locus_tag="JQG95_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708524.2"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY5889988.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAY"
BASE COUNT         1896 a         2301 c         2284 g         1994 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgctg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgtc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 tatcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctggggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct tacca
//
LOCUS       DADLCQ010000154         8412 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000154 DADLCQ010000000
VERSION     DADLCQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.156"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            128..1825
                     /locus_tag="JQG95_003560"
     CDS             128..1825
                     /locus_tag="JQG95_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY5889989.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQRLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="JQG95_003561"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="JQG95_003561"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY5889990.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="JQG95_003562"
     CDS             3115..3399
                     /locus_tag="JQG95_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY5889991.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQG95_003563"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="JQG95_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY5889992.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdxH"
                     /locus_tag="JQG95_003564"
     CDS             complement(4273..5157)
                     /gene="fdxH"
                     /locus_tag="JQG95_003564"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY5889993.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQG95_003565"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="JQG95_003565"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY5889994.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
BASE COUNT         2019 a         2253 c         2139 g         2001 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagcggc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gc
//
LOCUS       DADLCQ010000155         8396 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000155 DADLCQ010000000
VERSION     DADLCQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.157"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            4..817
                     /gene="ttdA"
                     /locus_tag="JQG95_003566"
                     /pseudo
     CDS             4..817
                     /gene="ttdA"
                     /locus_tag="JQG95_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            814..1419
                     /gene="ttdB"
                     /locus_tag="JQG95_003567"
     CDS             814..1419
                     /gene="ttdB"
                     /locus_tag="JQG95_003567"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY5889995.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1467..2930
                     /locus_tag="JQG95_003568"
     CDS             1467..2930
                     /locus_tag="JQG95_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HAY5889996.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="JQG95_003569"
     CDS             complement(2973..3986)
                     /gene="tsaD"
                     /locus_tag="JQG95_003569"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY5889997.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4224..4439
                     /gene="rpsU"
                     /locus_tag="JQG95_003570"
     CDS             4224..4439
                     /gene="rpsU"
                     /locus_tag="JQG95_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY5889998.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4550..6295
                     /gene="dnaG"
                     /locus_tag="JQG95_003571"
     CDS             4550..6295
                     /gene="dnaG"
                     /locus_tag="JQG95_003571"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY5889999.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6490..8331
                     /gene="rpoD"
                     /locus_tag="JQG95_003572"
     CDS             6490..8331
                     /gene="rpoD"
                     /locus_tag="JQG95_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY5890000.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2027 a         2254 c         2253 g         1862 t
ORIGIN      
        1 gcagtgaata agttgacaga gattgtcgct aactttaccg ccatgatttc tacccgaatg
       61 cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc gtcgatgggg
      121 aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct gaatcgtcct
      181 gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg cttcccactg
      241 cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca
      301 ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggtagc
      361 ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt
      421 tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat gccgtcagaa
      481 ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc
      541 tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc cgccgtactc
      601 tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa agcggcagaa
      661 ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgacc
      721 ggcaacagtt cagtgatggg cgcatcgtcg cggcacgctg ctggttcatg ccgatctcac
      781 ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa agatcctgac aaccccgatc
      841 aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgac cggtacgctg
      901 gtgacctgcc gcgacgtttg tcaccgccgt ttgatcgaac tgaaacgtcc gatcccttac
      961 gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa cggtgacaaa
     1021 tgggagatgg tctccgtcgg cccgacaacc agtatgcgta tggaaagttt tgaacgtgaa
     1081 tttattgagc agaccggcgt aaaactggtg gttggcaaag gtggtatggg gccgctgacc
     1141 gaagaaggct gccagaaatt caaggcgtta catgtgattt tcccagcagg ctgcgcggtg
     1201 ctggcggcaa cccaggtaga agagattgaa gaagtgcact ggacagaact cgggatgccg
     1261 gagtcactgt gggtctgccg ggtcaaagag ttcggcccgc tgattgtctc tattgatacc
     1321 cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg cgatcccatc
     1381 gtggaagaga tctgcgagca cgtccattac atcaaataac cctcccggag aggctcaccc
     1441 ctctcctttt tcgcaggcat aacacgatga aaccttccac tgaatggtgg cgatatcttg
     1501 cgccgctggc ggtcatcgcc attattgctc tacttccggt tcccgcaggg ctggagaacc
     1561 atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc ctcgaacccg
     1621 tgccgggtgc cgtggtggcg atggtgggta tctccatcat cgccatactc tctccctggc
     1681 tgctgttcag ccccgaacag ctcgcccagc caggctttaa gttcaccgcc aaatccctct
     1741 cctgggcagt ttctggtttt tctaattcgg ttatctggct cattttcgcc gcctttatgt
     1801 ttggcacagg ctatgaaaaa accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa
     1861 agatggggca tcgcacgctg tttctcggct atgcggtgat gttctccgag ctgatcctgg
     1921 cacctgtaac accgtccaac tcggcgcgtg gtgcgggtat tatctatccc atcatccgta
     1981 acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc attggctcgt
     2041 acatcatgtg gatggggatt gttgccgact gcgtgaccag cgccattttc ctgacggcga
     2101 tggcacctaa cttgctgtta attggactga tgaaaagcgc atctcacgcc acgctgagtt
     2161 ggggcgactg gttcctcggg atgttgccgc tcagcatttt actggttctg ctggttccct
     2221 ggctggctta cgtgctgtac ccgccggtac tgaagtctgg tgatcaggtg ccgcgctggg
     2281 cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc
     2341 tgatggtagg cgcgctggtg ctgtggattt tcggcggtga ttatatcgat gccgcgatgg
     2401 tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatcagttgg gacgacattg
     2461 tcagtaataa agcggcatgg aacgttttct tctggctggc ctcgcttatc accctcgcga
     2521 ccggactcaa caataccggt tttattagct ggtttggcaa actgttagca ggcagcttaa
     2581 gtggttattc gccgacgatg gtgatggtga cgttgattgt ggtgttttat ctactgcgct
     2641 actttttcgc cagcgccacg gcgtatacct gcgccctcgc accgatgatg atcgccgccg
     2701 cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggc gcggcaattg
     2761 gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac tacggtagtg
     2821 gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg atattcctcg
     2881 tgttgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg
     2941 tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc cgccagcggc
     3001 cagcgcggac gcacgctaac accgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt
     3061 ccggcatagg cgatcatcgc gccgttatca gtacaaaatt ccggacgcgc gtagaacact
     3121 tcgccgcggc gttttttcat catttcagcc agcttcgccc gtaacgtgcg gttagcactc
     3181 acgccgcccg ccatgaccag tcgcttaaag cccgtctgat ccaacgctcg cttacactta
     3241 atcatcaacg tatcgaccac cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg
     3301 tcgtcggtgc cgttgtcacg aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg
     3361 aaatccagcc ccggacggtc ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc
     3421 tgagccgcca ttttcgacag taacggtccg ccaggataat ccagccccag cagcttcgcg
     3481 gttttatcaa acgcttcacc ggcggcatca tcgatagact cgccgagcag ctcgtactga
     3541 ccaatgccag tcacgctgat taactgcgta tgaccgccgg acaccagcag cgcgacaaac
     3601 ggaaattccg gcgggttatc ttccagcatc ggcgctaaca gatgcccttc catatggtgt
     3661 accgggattg ccggaacgtt ccaggcaaac gccagagaac gccccacggt tgcgccaacc
     3721 agcagtgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat gtcttttgcc
     3781 gttaaaccag actcctttag cgccgcctgg atcaacggta cggttttacg cacatgatcg
     3841 cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac ctgactatac
     3901 aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag
     3961 gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta ccgcgcagtg
     4021 taggaccaat gcgggttgat gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac
     4081 tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa agcagcagca
     4141 attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag cggcaaaccg
     4201 aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac gagccgttcg
     4261 acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc
     4321 gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag
     4381 tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat
     4441 tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa
     4501 aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta tggctggacg
     4561 aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg atctgatcga
     4621 tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc cattccacaa
     4681 cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag ttttaccact gctttggatg
     4741 tggcgcgcac ggcaacgcga tcgacttcct gatgaactac gacaagctcg agttcgtcga
     4801 aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag caggcagcgg
     4861 ccccagccag atcgagcgcc atcagaggca aacgctttat cagttgatgg acggtctgaa
     4921 tacgttttac caacaatctt tacaacaacc tgttgccacg tctgcgcgcc agtatctgga
     4981 aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg attggttttg cgccccccgg
     5041 ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt cattgattga
     5101 tgcggggatg ttggtcacta acgatcaggg acgcagttac gatcgtttcc gcgaacgggt
     5161 gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg
     5221 caacgatacc cccaaatacc tgaactcgcc ggaaacagac attttccata aaggccgcca
     5281 gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt
     5341 cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg ccgttgcgtc
     5401 gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga ccaacaatgt
     5461 catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac
     5521 ggcgctgcct tacatgacag acggccgtca gctacgcttt atgtttttgc cggatggcga
     5581 agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc ggatggagca
     5641 ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg atctgagtac
     5701 ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ttgatatcgc aagtgccggg
     5761 cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca tacttgatga
     5821 cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc
     5881 gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa atccagaatt
     5941 agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg gacttggctt
     6001 attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg ggcaactttt
     6061 agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga tgtgggacga
     6121 tatagcagat aagaatattg ctgagcaaac cttcaccgac tcactcaacc atatgtttga
     6181 ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc atggtttaag
     6241 caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa agtgatttaa
     6301 cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag aggcagcggc
     6361 aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg gatttcagcg
     6421 ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga
     6481 taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag
     6541 gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat
     6601 tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca ggtgatggaa
     6661 gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga agatgctgcc
     6721 gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg
     6781 gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga aggcgaaatt
     6841 gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt tgctgaatat
     6901 ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt
     6961 ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct ggcacctacc
     7021 gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga agatgaagac
     7081 gaagaagatg gcgatgacga cagcgccgat gatgacaaca gcatcgaccc ggaactggct
     7141 cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg
     7201 aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga agtattcaaa
     7261 cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg cgtcatgatg
     7321 gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca gtgcaaaatg
     7381 ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac
     7441 gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg
     7501 catcgcgccc tgcaaaaact gcagcagatt gaagaagaaa ccggcctgac catcgagcag
     7561 gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa
     7621 gagatggttg aagcgaactt acgtctggtt atttctatcg ccaagaaata caccaaccgt
     7681 ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gcctgatgaa agcggttgat
     7741 aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg gatccgtcag
     7801 gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc gtattccggt gcatatgatt
     7861 gagactatca acaaactcaa ccgtatttct cgccagatgc tgcaagagat gggccgtgaa
     7921 ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg
     7981 ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga tgaagattcg
     8041 catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc tgcgaccacc
     8101 gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg tgaagcaaaa
     8161 gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga agaagtgggt
     8221 aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa
     8281 ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc tggacgatta atcggtaggc
     8341 cggatcaggc gttacgccgc acccggcact agtctctctg cacaaacgcc accttt
//
LOCUS       DADLCQ010000156         8260 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000156 DADLCQ010000000
VERSION     DADLCQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.158"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            66..314
                     /locus_tag="JQG95_003573"
     CDS             66..314
                     /locus_tag="JQG95_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY5890001.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            379..747
                     /locus_tag="JQG95_003574"
     CDS             379..747
                     /locus_tag="JQG95_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY5890002.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            841..1494
                     /gene="nfsB"
                     /locus_tag="JQG95_003575"
     CDS             841..1494
                     /gene="nfsB"
                     /locus_tag="JQG95_003575"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY5890003.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            1602..2849
                     /locus_tag="JQG95_003576"
     CDS             1602..2849
                     /locus_tag="JQG95_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY5890004.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="JQG95_003577"
     CDS             complement(2917..4293)
                     /gene="pheP"
                     /locus_tag="JQG95_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY5890005.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="JQG95_003578"
     CDS             complement(4394..7537)
                     /gene="cusA"
                     /locus_tag="JQG95_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY5890006.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="JQG95_003579"
                     /pseudo
     CDS             complement(7549..8067)
                     /gene="cusB"
                     /locus_tag="JQG95_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2013 a         2307 c         2013 g         1927 t
ORIGIN      
        1 gccgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
       61 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
      121 tcctctcttg cctgctgctg cccgcccccg cactggggct gacgctggca caaaaactgg
      181 tgaccatgtt ccatctgatg gatcttagcc agctttacac tttattgttt tgtctgtggt
      241 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
      301 cgctggcgga ttaaacgtcg attatcgccg aacttcgcgg catactttgc ttattctcct
      361 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctagct
      421 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
      481 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
      541 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
      601 tatcacatga ataaaaagca ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg
      661 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
      721 gatcaaaaaa gagtgcgtcc aggctaaagc gggaatctat agcgcatttt tctcgcttac
      781 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tacttacaca tggagtcttt
      841 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagcaaa
      901 aaacttaccc cggaacaggc cgagcagatc aaaacgctac tgcaatacag cccatccagc
      961 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     1021 gccaaatccg ctgccggtaa ttatgtgttc aacaaacgta aaatacttga tgcctcgcac
     1081 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     1141 caggaagatg ccgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     1201 cgcaagttct tcgccgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     1261 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     1321 gcggtgccca tcgaaggttt tgacgccgca atcctcgatg cagaatttgg tctgaaagag
     1381 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     1441 gctacgctgc cgaaatctcg tctgccgcaa aacaccacct taaccgaagt gtaattctct
     1501 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     1561 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     1621 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     1681 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     1741 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     1801 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     1861 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     1921 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     1981 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     2041 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     2101 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     2161 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     2221 ggctggaaat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     2281 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     2341 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     2401 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     2461 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     2521 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     2581 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     2641 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     2701 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     2761 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     2821 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     2881 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     2941 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     3001 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     3061 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa
     3121 acgcagatgc gccaggcaga tcataatcca gttcaacagc aacgttgcta ccaccagcgc
     3181 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     3241 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     3301 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     3361 cccgctgtta tacaccgaca gcgatgccac tagaatgacg aagttcagcg cagaagctac
     3421 cacattgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     3481 cacccacgga tagagtgcca gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg
     3541 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     3601 tgtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     3661 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     3721 tttctcgccg ccgtaaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     3781 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     3841 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     3901 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     3961 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     4021 ggcaaagtgg gcaaatgaac cggataccgg ctcttcgacc accatctcgc caagctggcg
     4081 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     4141 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     4201 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     4261 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     4321 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     4381 gaggtatcct gctttaattc cgtacccgat gtcggtgcag ccacatcagc ttatacgccg
     4441 ccgggataat aaacagcgac agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg
     4501 caatccggct catcacctct gaaccagccc ccgttcccca cagaatcggc agcagaccgg
     4561 cgataatcac cgccaccgtc atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca
     4621 acgcctcatc cagcttctgc tcgctgaatg tttgtggatt attcaacgac ggctcggcct
     4681 ctatggcgtg acgtaaatac atcagcatca ccacgccaaa ttcggcggcg accccggcaa
     4741 gggcaataaa gccggtaccc gtcgccacag aaagatgaaa gcccatccac cacagtaacc
     4801 agatgccgcc caccagcgca aacggtacgc tgctgataat cagcaacgct tcgcccaccc
     4861 gacggaatgc cagatacaac agcacgaaga tgatcatcag tgtcatcggc accatgagtt
     4921 tcagcttatg gttggcgcgc tccagtagct cgaactgccc ggagaatgcc acgctggtgc
     4981 ccggttttaa ctgcactttt tcagctatcg ctttttgcaa atcgtgaacc accgacacca
     5041 tgtcacgatc gcgggcatcg atataaatcc agctcgtcgg gcgcgcattc tcggttttca
     5101 gcatcgacgg tccggtagag actttaacgt cggccacgtc tgccagggtg atttgctgct
     5161 tcatcggcgt caggatcggc aactggcgca gtgcctgcgg actatcgcgc cagctttgcg
     5221 gataacgcag attaattgga taacgggcaa tcccttccac cgtttcgcca accatcgccc
     5281 cgcccaccgc agaagtcaca aacaactgca catccgccac cgtcataccg taacgtgcgg
     5341 ctttttcacg gttaatctca acgttgatat agcgcccacc ttccagccgc tcagcaagcg
     5401 cagaagctac gcctggcacc gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga
     5461 tatccgccag cacagtgccg gaaactttaa tgccgatggg gcttttaatg ccggttgaga
     5521 gcatatcgat acggttacga attggcggca cccacagatt cgccagcccc ggcagacgca
     5581 cggtgttatc cagttcctca atgattttgt ccattgtcat gcctggccgc cactgatcct
     5641 gtggcttaag ctggatggtc gtttctacca tctccagcgg agcagaatcg gtggcggttt
     5701 ccgctttccc ggttttgcca aatacccgcg ccacttcagg tacgctcata attagcttgt
     5761 cggttttttg cagcatactc gccgcctctg ctgcggaaat ccccggcagc gtcgatggca
     5821 tatacaacaa gtcgccttca ttgatctgcg gtaaaaattc cccgccaact ttattgagcg
     5881 gccagagaac cgtcagcacc gaaagcgccg ccaccagcag cgtggttttc ggccagtgca
     5941 gtactttcag caacagcgga tgataaacac gaatcaaaaa gcgattgagc ggattactgc
     6001 tttccggcgg aattttgcca cggatccagt aacccatcag gatagggatc actacgatcg
     6061 ccagcagcgc cgcacccgcc atcgcatacg ttttggtgaa cgccaacggg ccaaacagac
     6121 gcccttcctg cccttccagg gtgaagatcg ggataaacga caacgtgata atcagcagac
     6181 tgataaacag cgccggcccc acttcaacag acgcatcggt gatcacctgc cagcgcgttt
     6241 tattatccag cgtggcgtca ggatgctggt gctgccactc ttccagccgt ttatgcgcat
     6301 tctcgatcat gacgatagcg gcatcgacca tcgccccgac ggcaatcgca atgccaccca
     6361 gcgacataat attggcattc agcccctgga agggcatgac aataaaagca atacacaacc
     6421 ccagcggcaa cgaaataatc gccaccagcg ccgagcgcac atgccagaga aacagcgcgc
     6481 agactaccgc caccacaata aactcttcca gcaacttgcc gctgaggttg tcgatggccc
     6541 ggtcaatgag ctggctgcgg tcgtatgttg taactatctc cacgccttcc ggcagactac
     6601 ttttcagcgt ttccagtttg tccttcacgg cggcgatcac ttctcgggcg tttttgccgg
     6661 atcgcaggat caccaccccg cccgccactt cgccttcgcc gtttagttcg gcaatgcccc
     6721 ggcgcatctt cgggccaatc tggaccttcg caacatcgcg cagataaacg ggcacgccat
     6781 tttcactggc ttttaaaacg atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg
     6841 cgcgcaccat atattctgct tccgccagtt cgattgacga accgcccgct tcctggtttg
     6901 aagcatccag cgcgcttttt acttcggcga gactgatgcc atactgcgcc aggcgctgag
     6961 gatcgataac cacctgatac tctttcacca caccgcctac cgacgccact tccgcaacgt
     7021 caggaatggt tttcagctca tatttgagaa accagtcctg caatgagcgt aaatcggcaa
     7081 gatcgtgctt accgctgcga tccaccagtg catattcata gatccagcca acacccgtgg
     7141 catctggtcc cagctcggcg ctgactcccg caggcagctt accctgtacc tggttgaggt
     7201 actccagcac ccgcgaacgc gcccagtacg gatcggtgcc atcttcgaaa atgacataca
     7261 cataagagtc gccaaactgc gagaaaccac gcacagtctt cgcgccaggc accgacaaca
     7321 tggtggtggt tagcggataa gtcacctgat tttcaacgat ttgcggtgcc tggccgggat
     7381 agctggtttt aataatcacc tgcacatcgg agagatccgg caacgcatcc actggcgtat
     7441 taatgatggt ccaggtgccc cagatgctca gaaacaacgc gcccatcagc accaaaaaac
     7501 ggttcgccac cgagcgacga ataatccatt caatcattgg ttattccctc aatgcgcatg
     7561 ggtagcactt tcagagcgca tccgctccag tgcgccagaa atattggctt cggaatcaat
     7621 caggaacagg ccgccggaaa ccaccttttc accttccgcc agaccagagc gtaatgcggt
     7681 gacgccttgc gatgcctgga aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc
     7741 aacggtaatc acccgctgtt cgttgccggt atcaatcagc gcctgtgacg gaatgagcag
     7801 catcggttcg ctggcggtgt tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc
     7861 gtcggcgttg tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg
     7921 tagcagcgtc catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt
     7981 aaactgcgag gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca
     8041 caccgggtcc ataccctgaa ttcgcgatta cgcgcaatcc ccctgagaac cgccatgaac
     8101 aagggtttca tgatttgtga ttttagtttg gtacgcaatt tggtacacaa cactattttc
     8161 acttcagcgg gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca
     8221 ccacaaatat cacatctgga ctagttccgt cttcatgcgc
//
LOCUS       DADLCQ010000157         8208 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000157 DADLCQ010000000
VERSION     DADLCQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.159"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..304
                     /locus_tag="JQG95_003580"
     CDS             <1..304
                     /locus_tag="JQG95_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA packaging protein"
                     /protein_id="HAY5890007.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            276..2204
                     /locus_tag="JQG95_003581"
     CDS             276..2204
                     /locus_tag="JQG95_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAY5890008.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTRDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAASRKDATAQP
                     TLEELAAKLSGGVNGYSR"
     gene            2188..2391
                     /locus_tag="JQG95_003582"
     CDS             2188..2391
                     /locus_tag="JQG95_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890009.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="JQG95_003583"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="JQG95_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="JQG95_003584"
     CDS             3971..5476
                     /locus_tag="JQG95_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAY5890010.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSPGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="JQG95_003585"
     CDS             5513..5860
                     /locus_tag="JQG95_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY5890011.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="JQG95_003586"
     CDS             5918..6946
                     /locus_tag="JQG95_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY5890012.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="JQG95_003587"
     CDS             6998..7384
                     /locus_tag="JQG95_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY5890013.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="JQG95_003588"
     CDS             7396..7773
                     /locus_tag="JQG95_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890014.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8208
                     /locus_tag="JQG95_003589"
     CDS             7760..>8208
                     /locus_tag="JQG95_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890015.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPI"
BASE COUNT         1898 a         1933 c         2539 g         1838 t
ORIGIN      
        1 actcattgcc agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca
       61 ggtgtcaaaa ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc
      121 gatggcttta tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga
      181 tttaacgccg cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg
      241 cgcgcgtgcg ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga
      301 gtaatatgat ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga
      361 cggctgtaga atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg
      421 gggaatggaa aacgctgccg tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga
      481 ttcgtacagt taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg
      541 tgatcgggta ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact
      601 cagctgctga agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc
      661 tgaaaaaact ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc
      721 gtttttcctc tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg
      781 aaaaatctgt cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa
      841 aagaaggttc gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat
      901 ccatacgcgg ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg
      961 aatctgcgca tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc
     1021 tgaagtttgg cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa
     1081 cggtgtatta cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga
     1141 ccggggggcg ctggatttgt gacaataccg ggatgtggac gcgtgacggt ctgacgtttt
     1201 tcagtgcttc gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg
     1261 cgtacagtcc gttcaccacc tgggtacaga tagtctgtga ctggctggat gcactgaaag
     1321 atcccaacgg cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg
     1381 tgggcgaaaa actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg
     1441 tgcctgtccg ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga
     1501 tgtatgtctg ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca
     1561 tgggacgtcc tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat
     1621 accgccatgc agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga
     1681 tcgatggcga aatcgtgtat cagaggtcaa aaaaacacgg tgttttccgg gtactgccgg
     1741 taaaaggtgc ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc
     1801 ggggtgtgta tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa
     1861 tgaaggccga tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata
     1921 atccggagat tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa
     1981 agtgggaaaa aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc
     2041 tggactgcct ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg
     2101 atctggctgt gctggcggca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ccaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgtgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 ggctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagtcctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggcagcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacgacccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattga
//
LOCUS       DADLCQ010000158         7918 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000158 DADLCQ010000000
VERSION     DADLCQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.160"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            239..742
                     /gene="dps"
                     /locus_tag="JQG95_003590"
                     /gene_synonym="pexB"
     CDS             239..742
                     /gene="dps"
                     /locus_tag="JQG95_003590"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY5890016.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1146..1892
                     /gene="glnH"
                     /locus_tag="JQG95_003591"
     CDS             1146..1892
                     /gene="glnH"
                     /locus_tag="JQG95_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY5890017.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2031..2690
                     /gene="glnP"
                     /locus_tag="JQG95_003592"
     CDS             2031..2690
                     /gene="glnP"
                     /locus_tag="JQG95_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY5890018.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2687..3409
                     /gene="glnQ"
                     /locus_tag="JQG95_003593"
     CDS             2687..3409
                     /gene="glnQ"
                     /locus_tag="JQG95_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY5890019.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3831..5894
                     /gene="ybiO"
                     /locus_tag="JQG95_003594"
     CDS             3831..5894
                     /gene="ybiO"
                     /locus_tag="JQG95_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY5890020.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWGRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHMLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIMLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLHVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="JQG95_003595"
     CDS             complement(5891..6817)
                     /gene="rlmF"
                     /locus_tag="JQG95_003595"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY5890021.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7093..7353
                     /locus_tag="JQG95_003596"
     CDS             7093..7353
                     /locus_tag="JQG95_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY5890022.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7618..>7918
                     /locus_tag="JQG95_003597"
     CDS             7618..>7918
                     /locus_tag="JQG95_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY5890023.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1935 a         2026 c         1978 g         1979 t
ORIGIN      
        1 accgtattta tatatcgaat tatgcaaaag catatttatt ccgaaaattc ctggcgagca
       61 gataaataag aattgttctt atcaatatat ctaactcatt gaatctttat tagttttgtt
      121 tttcacgctt gttaccacta ttagtgtgat aggaacagcc agaatagcgg aacacatagc
      181 cggtgctata cttagtctcg ttaattactg ggacataaca tcaagaggat atgaaattat
      241 gagtaccgct aaattagtta aatcaaaagc gaccaatctg ctttataccc gcaacgatgt
      301 ctccgacagc gagaaaaaag caacagtaga gttgctgaat cgccaggtta tccagtttat
      361 tgatctttct ttgattacca aacaagcgca ctggaacatg cgcggcgcta acttcattgc
      421 cgtacatgaa atgctggatg gcttccgcac cgcactgatc gatcatctgg ataccatggc
      481 agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc actcaagtta tcaacagcaa
      541 aaccccgctg aaaagttacc cgctggacat ccacaacgtt caggatcacc tgaaagaact
      601 ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga
      661 tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt
      721 tatcgagtct aacatcgaat aaatccatcg ctgatggtgc agaactttag tacccgataa
      781 aagcggcttc ctgacaggag gccgttttgt tttgcagccc acctcaacgc acttatttag
      841 tgcatccatc tgctatcccc agctgattaa gtaaattttt tgtatccaca tcatcacaca
      901 atcgttacat aaagattgtt ttttcatcag gttttacgct aaataatcac tgtgttgagt
      961 gcacaatttt agcgcaccag attggtgccc cagaatggtg catcttcagg gtattgccct
     1021 ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc aatgagttgc aaaattggca
     1081 cgattttttc atatatgtga atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa
     1141 atgctatgaa gtctgtatta aaagtttcac tggctgcact gaccctggct tttgcggttt
     1201 cttctcatgc cgcggataaa aaattagttg tcgcgacgga taccgccttc gttccgtttg
     1261 aatttaaaca gggcgataaa tatgtgggct ttgacgttga tctgtgggct gccatcgcta
     1321 aagagctgaa gctggattac gaactgaagc cgatggattt cagtgggatc attccggcac
     1381 tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac catcaccgac gagcgtaaaa
     1441 aagcgatcga tttctctgac ggctactaca aaagcggcct gttagtgatg gtgaaagcta
     1501 acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg
     1561 gtactggctc cgttgattac gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc
     1621 cgaacatcga taacgcctat atggaactgg gcaccaaccg cgcagacgcc gttctgcacg
     1681 atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag
     1741 gtgactctct ggaagcgcag caatacggta ttgcgttccc gaaaggtagc gacgagctgc
     1801 gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct
     1861 acaaaaaatg gttcagtact gaaccgaaat aataacgcta cacctgtaaa gcgcactggc
     1921 agttccctct cccccctggg gagaggatta gggtgagggg cgcaaacccg ctccggggcc
     1981 attaattacc ctgaatttga ttatttacac cacggtaaca ggaacaacat atgcagtttg
     2041 actggagtgc catctggcct gccattccgc ttctgattga aggtgccaaa atgaccctgt
     2101 ggatttcggt cctcggtctg gctggcggtc tggtaatcgg attgctggca ggttttgcac
     2161 gcaccttcgg tggttggata gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg
     2221 gcacacctat cgtcgtccag gtgatgttta tctatttcgc cctgccgatg gcgtttaacg
     2281 acttacgcat cgacccattt actgcggcgg tggtcaccat catgatcaac tccggcgcgt
     2341 acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca caaaggtttt cgtgaagcag
     2401 gactggcgct cggtctttca cgttgggaaa ccattcgcta cgtcatttta ccgctggcac
     2461 tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat cagcattaaa gacacctcgc
     2521 tgtttattgt tatcggcgtg gcggaactga cccgtcaggg gcaagaaatt attgccggta
     2581 acttccgcgc ccttgagatc tggagcgccg tggcggtgtt ctatctgatt attaccctgg
     2641 tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa
     2701 aaacgtctcc aagcactttg gcccaaccca ggtgctgcac aatatcgatt tgaacattgc
     2761 ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg
     2821 ctgcatcaac aaactggaag aaatcacctc cggcgatctg attgtcgatg gcctgaaggt
     2881 caacgatccg aaagttgacg agcgcctgat tcgccaggaa gcaggtatgg tgttccagca
     2941 gttttacctc ttcccgcatc tgacggcgct gggaaacgtc atgtttggtc cgctacgcgt
     3001 gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg
     3061 tctggcagaa cgtgcacatc actacccttc cgaactttct ggtggtcaac agcagcgtgt
     3121 ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc
     3181 cgctcttgac ccggaactgc gccatgaagt gctgaaggtt atgcaggatc tggctgaaga
     3241 agggatgacg atggtgatcg tgacccacga aatcggtttt gccgagaaag tagcttcgcg
     3301 gctgatcttt atcgacaaag gccggattgc ggaagatggc aatccgcagg tgttgatcaa
     3361 gaacccgccg agccagcgct tgcaggaatt tttgcagcac gtctcttaat aagacacatt
     3421 gcctgatgcg ctacgcttat caggcctaca ggatatctgg caacttatta aaattgcatg
     3481 aacttgtagg acggataagg cgttcacgcc gcatccggca aaaaagcccg cacgttgtca
     3541 gcaacctgct taatatccct tcctcccttt cacccgaaag ggaggcacac cagattcctc
     3601 tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact
     3661 ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac
     3721 gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc
     3781 ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc actggcggat gtgctggata
     3841 acgacacctc gcgtaaagag ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg
     3901 cggaaccggt cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa
     3961 aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt
     4021 atcgcaatat caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc
     4081 tgagccattt ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt
     4141 gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca
     4201 gcaactggtt gcagcttccg gcgatgatta ttggggcgtt tattatcgac ctgctgttac
     4261 tggccctgac attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca
     4321 ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag
     4381 ccgtactacg cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg
     4441 acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt aagcagcctg attggctatg
     4501 gcctgattgt ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg
     4561 cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt gtacctgatc ttccgaaata
     4621 aaaaagagat tacccagcat atgctcaact ttgcggagca ttcgctggct tttttcagcc
     4681 tgtttatccg cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcatgc
     4741 tgtttttctt ttcgttgttc gatccgggca acagtctgaa atttatgatg ggtgcaacgg
     4801 tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc
     4861 tggcgaaaac catcaccctc tcgccacata ctcagcgtaa ctatccggag ctacaaaaac
     4921 ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg
     4981 cagtaatgtt gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg
     5041 gcgcggggca gaaaaccgta gatatcctga tccgcatcgc actcattctt ttcttctcgg
     5101 cggttggctg gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg
     5161 gccgcccgct acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg
     5221 cggtgattat cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg
     5281 cgccattgct ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc
     5341 tggtaaaaga tattatcacc ggggtattta ttcagtttga aaacggcatg aacactggag
     5401 atttggtgac tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg
     5461 gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg
     5521 ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag
     5581 atgctgataa agccaatcag gcactgaaag atgcggtaac ggaattgatg gaaaacgaag
     5641 aaattcgcgg gctgattatt ggtgaaccga actttgccgg gattgtcggc ttaagcaata
     5701 ccgcgtttac actgcatgtt tcgttcacca cgctgccact caaacagtgg acggtacgct
     5761 ttgccctcga cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc
     5821 agacttatca ggtgctgcct gctccgggcg cgaccccggc tgaaccgctg ccgccggggg
     5881 agccaacgct ttaacgttgg cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg
     5941 tccaggcaat aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca
     6001 ccgcgcccac gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca
     6061 gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga
     6121 taaagttgac ttcaccgcct tcacaccaca actcctgttg ctgaccacca aagttcagtg
     6181 catcgtcttt gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgccgcag
     6241 cggcggaatc gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttgtgga
     6301 tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa
     6361 gccccggatt agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac
     6421 taccggtaaa acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac
     6481 caacgccgat atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta
     6541 aatcggcaag gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt
     6601 cggggatatc ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct
     6661 tcaccgccag cggattggca aagtctacgc tttgctcccc tgcgggtgta agcgtgagga
     6721 attgcctgag ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac
     6781 ggttgcgcgg atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc
     6841 gtaagatacc cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa
     6901 ccttaacatg gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt
     6961 gatttgtctt attttgtaaa aatatatcgt tcaaaaaata agcatatctc tgtcaatttt
     7021 ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat
     7081 gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc
     7141 tctctcacgg cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc
     7201 gccggtaccg tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca
     7261 gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac
     7321 cagatgttag gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc
     7381 tggatgcagt tttcgcctgt tttgtaatca aaaaacatca aatatgaacg caatgtaaat
     7441 aaatgtattt ctttttcgcg caatgggtga tagaaaatcg catcaagtga taatgcttat
     7501 caaaattact atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca
     7561 gagatgcttt ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg
     7621 gaaaacaatc gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt
     7681 tgtattggta ttacgcctgt ggcacaggca ctcgccgccg aagggcaagc taacgcggat
     7741 gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat
     7801 ccgaaattct cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa
     7861 gtgattaaag atcagggcgc aaccaacctt accgacgcgc tcaaaaacgt ccccggcg
//
LOCUS       DADLCQ010000159         7910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000159 DADLCQ010000000
VERSION     DADLCQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.161"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            31..1388
                     /gene="yjeM"
                     /locus_tag="JQG95_003598"
                     /pseudo
     CDS             31..1388
                     /gene="yjeM"
                     /locus_tag="JQG95_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="JQG95_003599"
     CDS             complement(1539..4850)
                     /gene="mscM"
                     /locus_tag="JQG95_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY5890024.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4872..5840)
                     /gene="psd"
                     /locus_tag="JQG95_003600"
     CDS             complement(4872..5840)
                     /gene="psd"
                     /locus_tag="JQG95_003600"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY5890025.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="JQG95_003601"
     CDS             complement(5937..6989)
                     /gene="rsgA"
                     /locus_tag="JQG95_003601"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY5890026.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7084..7629
                     /gene="orn"
                     /locus_tag="JQG95_003602"
     CDS             7084..7629
                     /gene="orn"
                     /locus_tag="JQG95_003602"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY5890027.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1794 a         2057 c         2135 g         1924 t
ORIGIN      
        1 aagcacctta aaatcaccat catacactaa atcagtaagt tggcagcatt acccgcctta
       61 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
      121 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      181 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      241 ggtagtgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      301 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      361 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      421 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      481 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      541 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      601 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      661 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      721 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      781 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      841 accggacttt cgatgttcct cgcttatacc ggtgcgtttg ctattcaccg ctgaaagcca
      901 tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg aatgcgatgg
      961 ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc attctgctgg
     1021 tttcgtttgg tggcggtacc gcatcggcgt tctttaacaa gctgacgctg atggcgaacg
     1081 tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccgtttttt aaagcacgtc
     1141 aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg
     1201 tggttgtcgt tctggtggtg acatttgcga acgtcttcac cattattcag cctgtggttg
     1261 aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt
     1321 tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc
     1381 tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag
     1441 taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct
     1501 aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga
     1561 cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc
     1621 atctggaagg gcgggaatgg catatcgata ccgtgggcgt ggaagcctgc cagaatcagc
     1681 tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcgta aatacgcagc
     1741 tcgaaaatct gaatcccctg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg
     1801 atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg
     1861 gcatcggcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga gagcgaccag
     1921 ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag
     1981 tcgctgatgg tggtggcgcg ggtgttaatt tttgtcacgc taccagtaag atcgcggatc
     2041 gttaccgtat cgccaatgcg aatcggtttt tcgaacagga tgatcaggcc agagataaag
     2101 ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc aaccagccac
     2161 tgcaatttcg accactcaat accaatcatt gagaagccga ccagcccgcc aatcagcatc
     2221 agcagatatt ttgtgatggt ggtgatggcg taacccgtac ccggcgttaa atccaggtgc
     2281 tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata
     2341 aataccagaa tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgccc
     2401 tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg
     2461 atttctgacc acagcacaat gaccgaaagc agggcgatca acatcaaaat tgagcgcacc
     2521 agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt
     2581 gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac
     2641 atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcgacgctg gatcagcatc
     2701 cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca
     2761 agcctcgcta acagtgcctg cgccgttgcc agataaccca ccgccgacgc cagaatggca
     2821 accagtggtg cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg
     2881 ccttctttgt tgagatacaa cggaataccg gcttttttca ggcttagggt gaccaccgcc
     2941 agcgcaccgc aaatgaggat aaagcaaagc cgtcccagcg aaccggaaaa ttcgcggtcg
     3001 tcgaggttat cgaacatcat cagcgccata atcagcccga tgctcatcag gtagtagcgc
     3061 atcccacggg aaacacgttc gcgcggccag ccaaaatgag cgataaacaa gccgttcggg
     3121 cgggcaaagg tggcgcaaat catcactacc cacagcagcg gcacggtggc cgttacacca
     3181 tcgccaatcg cgaccgccag cggataaggc cacgcctcgc gcaagccgta acccagcgtc
     3241 atccacagca ccggtaacgg tgacgcgacg agaatcgacc agaaaagagt gcgcaacgtc
     3301 agccagaagt gatcctgagt cactttgccg actttcgccg ccgaacgttc aagaaaacgg
     3361 gtgaaatagc ggcgcgagta aatactgcaa ccgaccagaa tcaacgcgcc aaacagcggc
     3421 aaaattgtct ctttgctggt cagcatcatc acactggctt tgcccaactg actgaaggtg
     3481 tccagcgaaa tgagacgacg cagatcctgg gcgatttcca gcggccaggc gatggtcatc
     3541 gggcgcacgt cagaggccca gaacagatag cggtgcgttg cttcgttcac ctctttcagc
     3601 gcatcctcca gttgcccgtt ggagactttc agcttggtca gttccaatag tagcgtgtcg
     3661 ccaccctgca acaatgagtt cagcaactca cgctgagtgc gcagttgtgc ttccagaata
     3721 cggttttgct cggcggtcag cggctgaccg tcggcctgat ggatttgccg tagcagcggc
     3781 tgtttattaa gtaaatcctc ataacgtaac cgttgcacac gcaactgcgc catttcggta
     3841 tcaagctgtt gtggttttgg catttctggt agccgtgcca cctgcgcccg cagcgcttcg
     3901 ccgagcaggt tggacgatcc cagccattgc gactgttcac gcagcgtatt caacgcctgc
     3961 cggacctgta acgtctggct ggcagcctga cgctgttgcg aggcaacgag ctccatccgc
     4021 tgcgcctgtt gattcagcgc tgccgatagt tcgcggttaa ttttgaattg cgcgacgata
     4081 tctttcggca aatcggcact gttttctgcc agtaattcgg tactttccag cgcccgctcc
     4141 gcctcaagct gacgttggct attcaactga ttacgtaagg cctgcaaata cgcatccagt
     4201 tgctggctct ctttttccgc cagctctgag cgtaagcgcg ctaattcctg gcggttattg
     4261 gcagacagct gcgccagctc cagttcatca acgagcgcct taagacgtgc agagtcagac
     4321 tgcaacgcga aattttgtgc ctgattgagc ggagtattgc cggtaagcgt tcccaggcgg
     4381 cgctcgatct catttaactg acggcgggcg tcggtttgct gttgcggcag ttgattcagc
     4441 gaatcggcaa tctcgcgggc tcgctcctgc tcttgctggg cctgacggct tttatccagc
     4501 aactggctgc tgacctggag aatttcctga ttcagcgcgt cggtagacat tcccggcgac
     4561 acgctgcgcg gctcgtcacg aatgttgttt aattgtgcgc gcagagtagc ggagagtttc
     4621 ggataattat cgataacttg ctgatattgt ttgatgcgct caagggaacc ttttcgttcc
     4681 tcaagcgcat ttaaggcaga ctggagcgcc tctacgactt ccggctgtgc gggtttcgcc
     4741 gcttttgcct gctccagttc ctgagtgatt tgtttgctat cgggggccgt cgcggcgtac
     4801 gccccccaac tgaggcacca ggccatcaga aaagtgataa tcaggcgcac gtcagcgttt
     4861 cctttgatgg attagacctg gtctttttta tcgtcaacca atgggctggc gtcgtgttct
     4921 gcttcgatct cttcagcagg aagtggggcg ggttcagcgt ctggcgtaac aaaggtttcg
     4981 gtagatactg ccagcggctg gccaattttc gtgacagaca ggctttccag ttgctcaacc
     5041 agattcactt tacccggtgc aaacaggttg ataacggtgg aaccgagttt aaagcgaccc
     5101 atttcctggc ctttcagcag tgccacagaa ccgtcgtttt ccccggcagg ccaggtccag
     5161 cgcttgatga taccttcgcg cggcggcgta atggtgcccg cccagaccgt ctcaatgctg
     5221 ccaacaatcg tcgctccgac cagaatctgc gccattgggc caaattcggt atcgaaaagg
     5281 caaatcacgc gttcgttacg ggcaaacaga ttcggcacgt tctgagcggt gagatggtta
     5341 acggaaaaga gatcgcccgg cacgtagatc atctcacgca gaataccgtt gcacggcatg
     5401 tgtacgcgat ggtagtcacg cggggagagg taagtggtca caaacgtacc gttgcggaac
     5461 aggtccgcca tcagatagtt gcctgccagc agggcttcga ggctgtagtt gtggcctttg
     5521 gcttgcagga ttttatcttc ttcgatttta cccaactggc tgataacgcc atcggcaggc
     5581 atgaccagta cattcggatc ggtatcgatt gggcgtactt cgtcacgcag cggacggaca
     5641 aagaattcgt taaaggtgcg gtagctggcg gtgtccggct tttgcgcctc tttcatgtcg
     5701 accttgtagt atttaacgaa cagatcgata accagttttg tcagccatcc tgcccgcttg
     5761 cttgcgcccc aacccgccag gcgagtaagc catagtttcg gcagaatgta ctgtagcgaa
     5821 agtttaaatg aatttaacaa ggtagcctcc aggccattgt tttgtcgttc ctgatccggc
     5881 caacatgccg gatcctgaaa aaaaggggac gattctaacg acggttagct taattgtcag
     5941 tcatccgtat cagaaaagtt tttacgcgtt tttacctgcg ccatgctttc cagaatacgg
     6001 tgatagtttt cgaaacgggt ttccgcgatt ttcccttcct caaccgcttc ccggatagcg
     6061 cagcccggat cggtatcgtg tttgcaatcg cgatatttac acagacctaa atagtcatgg
     6121 aattcgacaa agccctgagt gatttgttcc ggctccaggt gccagaggcc gaactcacgc
     6181 acaccagggg aatcaatcac atcaccgccg tgcgggaagt gatacagccg agcggcggtc
     6241 gtggtgtgct gaccgagccc cgagttgtca gagacatcgt tggtcaggat ctctttttgc
     6301 agccccagca acgcgttcag caggctggat ttaccgacgc cagactgccc ggcaaaaatg
     6361 ctgatgcgcc cggtcaacgc ctcttccagc ggtttcagcc catcctgagt atggctggaa
     6421 accatcaata cgcgataacc gatgttgcgg tagatatcca tctgctcgtt gacgaacgcc
     6481 atgccttcgt cgtccagcag gtctatcttg ttgagcacga taatcggctc aatctgcaag
     6541 gtttcgcagg ccaccaggta acggtcgata atattgagcg acagctccgg caaaatggcg
     6601 gagacaatga caatctggtc gatgttggcg gctatgggtt tcacgccgtc gtagaaatcc
     6661 ggacgcgtta acaccgaggt gcgctcatgc accgcttcca cgatcccttt gacatttacg
     6721 ccttccgccg ccggtttacc cggacgccag actacgcggt cgccggttac cagcgaacgg
     6781 atggtacggc gaatattgca gcggtgaacg tcgccatcgg cggattccac atcagcgtgc
     6841 ataccaaagc ggctgatgac gataccttca tcaggctcgc caaacagatt gtcgtcgtag
     6901 tcgggcttct ccttagacgt tttaagacga cgctggtgat tggcgttcac gcggcgctgc
     6961 tggcctttgg agagtttatt tttactcaat cgtactgact cctggtcgcc cctgatgggc
     7021 aaaacatcta tgatacacgc aattgtggat caatatagtc actgtgaatg ggtggaaaat
     7081 agcatgagtg ccaatgaaaa caacctgatt tggatcgatc ttgagatgac cggtctggat
     7141 cccgagcgcg atcgcattat tgagattgcc acgctggtga ccgatgccaa tctgaatatt
     7201 ctggcagaag ggccgaccat tgcagtacat cagtccgatg aacagctggc gctgatggat
     7261 gactggaacg tgcgcaccca taccgccagc gggctggtag agcgcgtgaa agcgagcacg
     7321 atgggcgatc gggaagctga actggcaacg ctcgaattct taaaacagtg ggtgcctgcg
     7381 ggaaaatcgc cgatttgcgg taacagcatc ggacaggacc gtcgtttcct gtttaaatac
     7441 atgccggagc tggaagtcta cttccactac cgttatctcg atgtcagcac cctgaaagag
     7501 ctggcgcgcc gctggaagcc ggaaattctg gatggtttta ccaagcaggg gacgcatcag
     7561 gcgatggatg atatccgtga atcggtggcg gagctggctt actaccgcga gcattttatc
     7621 aagctgtaaa atttttcctg gtcacgtaag cgccggaatc cacttgcggc ggcgtgattt
     7681 tgacgctaaa tcgaacgtct tgctgataat ttcagcactt gagataaaaa cgcaaaaaaa
     7741 acttttttgg ggggttgcag agggaaagat ttctcgtata atgcgcctcc cgtaacgacg
     7801 cagaaatgcg aaaattacga aagcaaaatt aagtagtacg cgggaatagc tcagttggta
     7861 gagcacgacc ttgccaaggt cggggtcgcg agttcgagtc tcgtttcccg
//
LOCUS       DADLCQ010000160         7859 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000160 DADLCQ010000000
VERSION     DADLCQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.162"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            81..408
                     /locus_tag="JQG95_003603"
                     /pseudo
     CDS             81..408
                     /locus_tag="JQG95_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="JQG95_003604"
     CDS             complement(438..3350)
                     /locus_tag="JQG95_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890028.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="JQG95_003605"
     CDS             3353..3469
                     /locus_tag="JQG95_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY5890029.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="JQG95_003606"
     CDS             3833..4675
                     /locus_tag="JQG95_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY5890030.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="JQG95_003607"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="JQG95_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY5890031.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="JQG95_003608"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="JQG95_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY5890032.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="JQG95_003609"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="JQG95_003609"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY5890033.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1761 c         1860 g         2122 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DADLCQ010000161         7780 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000161 DADLCQ010000000
VERSION     DADLCQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.163"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            54..587
                     /gene="ymdB"
                     /locus_tag="JQG95_003610"
     CDS             54..587
                     /gene="ymdB"
                     /locus_tag="JQG95_003610"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY5890034.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            529..2010
                     /locus_tag="JQG95_003611"
     CDS             529..2010
                     /locus_tag="JQG95_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY5890035.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="JQG95_003612"
     CDS             complement(2018..3175)
                     /gene="mdoC"
                     /locus_tag="JQG95_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY5890036.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3569..5104
                     /gene="mdoG"
                     /locus_tag="JQG95_003613"
     CDS             3569..5104
                     /gene="mdoG"
                     /locus_tag="JQG95_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY5890037.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5097..7640
                     /gene="mdoH"
                     /locus_tag="JQG95_003614"
     CDS             5097..7640
                     /gene="mdoH"
                     /locus_tag="JQG95_003614"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY5890038.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1926 a         1862 c         2058 g         1934 t
ORIGIN      
        1 taaccaaaaa gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa
       61 cgcgtattca tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg
      121 cggctaatcc gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg
      181 gtccggccct gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg
      241 ggcatgccgt tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg
      301 ggccagtctg gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga
      361 atagcctacg actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta
      421 ctggggttta tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag
      481 aatttattac ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa
      541 acgcccacct ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg
      601 gcgagcgcgg tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg
      661 gagaaaagtc tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg
      721 ctggatgttc agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc
      781 ctgttagccg cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg
      841 cccggacttg acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt
      901 tttaatcctt tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt
      961 ctcaatcgcc ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga
     1021 ggacgaaata ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta
     1081 gatgtcatgg caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg
     1141 tattgcaaat cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg
     1201 gatcgcatcg agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc
     1261 aaaatggaat ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg
     1321 gcgaagacac gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat
     1381 tcactgttac cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt
     1441 atctcttcct attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga
     1501 aaaggggtaa agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc
     1561 catgccggat acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa
     1621 ctcaagccga cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc
     1681 ggtgccagcc tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct
     1741 ttcaatttcg atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc
     1801 gaaacgctgg cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg
     1861 cagctccgtc tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag
     1921 gttattctca aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg
     1981 tcgatattgc ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt
     2041 tctcacgctt aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat
     2101 gaatttcata cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa
     2161 gccagttgga ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt
     2221 gaaccagata gataaacagc gatgcgttaa caaaataagt cacccgtgct gactggaagt
     2281 taagcaaacg gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg
     2341 tgatcaccga ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta
     2401 aataagcgac aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca
     2461 aggctttaag atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa
     2521 atagcgtttg catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa
     2581 taaaaatcgt tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata
     2641 gttttaccat cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc
     2701 gcttaaatat ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt
     2761 gtgatattaa ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac
     2821 tttccgcttt ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga
     2881 tggctgttaa catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg
     2941 gatagcgtaa aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc
     3001 ggaacgagtg gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca
     3061 catgccatgt atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca
     3121 gccaggcgcg gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg
     3181 gttaactaat ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc
     3241 catagcgaca ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc
     3301 atcgctacca ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg
     3361 caaaccctac acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt
     3421 tgcaatcagg tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg
     3481 gaatggctgg ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg
     3541 cttactaatt atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt
     3601 aatgttaacc ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca
     3661 agctcaatcc ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt
     3721 ccgcgacatg aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg
     3781 gaacaatctg aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac
     3841 cccggtcaaa ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga
     3901 ttatttcact ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc
     3961 cggttttaaa gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat
     4021 gctcggggcc agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg
     4081 cggcctagca attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt
     4141 ctggatcgag cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc
     4201 gccgcgtgcg acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga
     4261 tgtgcagtcg aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac
     4321 cagtatgttc ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt
     4381 gcacgactct aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt
     4441 gaataacccg aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg
     4501 tctgttgcaa cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct
     4561 ccgtccaagc gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt
     4621 ggaaattcca accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca
     4681 gctgccggag ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga
     4741 agacaaactg catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga
     4801 tgtgaagcag tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt
     4861 taccggcgca gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat
     4921 tggtgataat ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg
     4981 gcgtctggtg atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc
     5041 gctggtgaat gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga
     5101 ataagacaac tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc
     5161 cgaagactga tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc
     5221 gggaggatga ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt
     5281 cacttgctga tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc
     5341 cagaagcaaa acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt
     5401 tctgggatcg cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag
     5461 aagagcagga gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt
     5521 tgatcctgac gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt
     5581 atcagggttg ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct
     5641 ttatgcagct tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt
     5701 tctgttgggt gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg
     5761 gtcgcgataa atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc
     5821 atcgcacggc gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct
     5881 tgcgtgcaac gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca
     5941 ttctgagtga cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc
     6001 ttatcgctga agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga
     6061 agcgtaaaag cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca
     6121 tggtggtgct ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct
     6181 tgatggaagc caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg
     6241 atacgctgta tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag
     6301 ccggtttgca cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc
     6361 gcgtgaaacc atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg
     6421 ccggttcaat cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg
     6481 gggtctggat tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg
     6541 atgagctaaa acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc
     6601 tggttaaggg tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc
     6661 tctccgctcc gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg
     6721 cgttgaccga accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt
     6781 ggcgtcctga gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga
     6841 agttattgag cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc
     6901 gcgttacatt atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca
     6961 tgctgttcca tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt
     7021 caccgcagcg tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac
     7081 agctgctgtt ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt
     7141 tctggctggc accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca
     7201 gccgtgccac cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt
     7261 attcaccgcc gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct
     7321 cccttgacga tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg
     7381 caatggcgac agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg
     7441 ttgaacaggc gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc
     7501 taagcgatcc ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat
     7561 attcttcatg ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac
     7621 cggatgcggc ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca
     7681 tcgttcctgc aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca
     7741 catccggcaa aaacaacgag ccaataaaaa taccggcgtt
//
LOCUS       DADLCQ010000162         7743 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000162 DADLCQ010000000
VERSION     DADLCQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.164"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1013
                     /locus_tag="JQG95_003615"
     CDS             <1..1013
                     /locus_tag="JQG95_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890039.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1102..2562
                     /locus_tag="JQG95_003616"
     CDS             1102..2562
                     /locus_tag="JQG95_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY5890040.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="JQG95_003617"
     CDS             complement(2598..2801)
                     /gene="ydfZ"
                     /locus_tag="JQG95_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY5890041.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2979..3665)
                     /locus_tag="JQG95_003618"
     CDS             complement(2979..3665)
                     /locus_tag="JQG95_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5890042.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="JQG95_003619"
     CDS             complement(3754..4500)
                     /gene="ydfG"
                     /locus_tag="JQG95_003619"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY5890043.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4637..6682
                     /gene="dcp"
                     /locus_tag="JQG95_003620"
     CDS             4637..6682
                     /gene="dcp"
                     /locus_tag="JQG95_003620"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY5890044.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQLRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6727..7245)
                     /locus_tag="JQG95_003621"
     CDS             complement(6727..7245)
                     /locus_tag="JQG95_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY5890045.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7521..>7743
                     /gene="ydeI"
                     /locus_tag="JQG95_003622"
     CDS             7521..>7743
                     /gene="ydeI"
                     /locus_tag="JQG95_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY5890046.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1812 c         1954 g         2036 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctagcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat
     1081 atttaaattt aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct
     1141 gtttatgatc gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac
     1201 cgtgcgcatc agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga
     1261 tattatgagg tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat
     1321 aatctttata ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc
     1381 gttaaaaaag ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt
     1441 gaaccgcaaa tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg
     1501 gcgaccggac agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac
     1561 cagccgaaaa cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga
     1621 cttcccgcat ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt
     1681 gacgttgtca cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat
     1741 aacgtgactt tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg
     1801 ctggcgaaaa tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct
     1861 ttccgccagt gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg
     1921 ggagccgaac tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac
     1981 ggcagtcatt cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac
     2041 tgtatggaag atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg
     2101 ccgacgctga aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc
     2161 tatagcaacc cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa
     2221 ttgccacagc ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat
     2281 ctgctggcgc tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga
     2341 aatccgatag aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt
     2401 gccgaaggga aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat
     2461 ttgccagaca atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg
     2521 catggcgcga aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat
     2581 cggcggcatt cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc
     2641 tcttcacaac cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct
     2701 tcagtatcaa tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg
     2761 ccagtggtga ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg
     2821 atttacagtc gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt
     2881 ttttgttttt gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg
     2941 aaaagcctct catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg
     3001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
     3061 gcacgttcta ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg
     3121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
     3181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
     3241 agttgatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
     3301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
     3361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
     3421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
     3481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
     3541 gaaacttctt tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga
     3601 cgaagaatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
     3661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
     3721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
     3781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
     3841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
     3901 ggttttttcc gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
     3961 caccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag
     4021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
     4081 cggccagctg cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
     4141 acccggtaag acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt
     4201 ttcccagtct tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
     4261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
     4321 agcggcgcgg ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt
     4381 taactcctgc aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
     4441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
     4501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
     4561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
     4621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
     4681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
     4741 aatgcaacaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt
     4801 caacaatact attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt
     4861 cttttttgcg atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt
     4921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg
     4981 ggtagatgct gtctggcagc tccgtgaatc cctggggctt gatagtgaat ccatccgcct
     5041 ggtggaggtt attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa
     5101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
     5161 gttacttgca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
     5221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
     5281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
     5341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
     5401 caatgatacc cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact
     5461 gcttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga
     5521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
     5581 attagcctcc atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg
     5641 ggactgggca ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca
     5701 gctcaagcca tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa
     5761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt
     5821 tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt
     5881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac
     5941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
     6001 tgagcctgcg ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac
     6061 gctgcacggc ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg
     6121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
     6181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
     6241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
     6301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
     6361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
     6421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta
     6481 tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt
     6541 aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
     6601 tctggaacgc ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca
     6661 acatcgtggc ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca
     6721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
     6781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
     6841 ccacgcgaac cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta
     6901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc
     6961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag
     7021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
     7081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
     7141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
     7201 gtcggtatct gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg
     7261 ctattatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa
     7321 acatataaat ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag
     7381 tgagtcaaat tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa
     7441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
     7501 aacgcaatta aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca
     7561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc
     7621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
     7681 tcgcaaaaaa catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
     7741 aag
//
LOCUS       DADLCQ010000163         7719 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000163 DADLCQ010000000
VERSION     DADLCQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.165"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..830)
                     /locus_tag="JQG95_003623"
     CDS             complement(<1..830)
                     /locus_tag="JQG95_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HAY5890047.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     "
     gene            1012..2436
                     /gene="sbcB"
                     /locus_tag="JQG95_003624"
     CDS             1012..2436
                     /gene="sbcB"
                     /locus_tag="JQG95_003624"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY5890048.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2479..2706)
                     /locus_tag="JQG95_003625"
     CDS             complement(2479..2706)
                     /locus_tag="JQG95_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY5890049.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="JQG95_003626"
     CDS             complement(2720..3778)
                     /gene="yeeE"
                     /locus_tag="JQG95_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY5890050.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="JQG95_003627"
     CDS             complement(3957..5315)
                     /gene="plaP"
                     /locus_tag="JQG95_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY5890051.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="JQG95_003628"
     CDS             complement(5305..5367)
                     /gene="yoeI"
                     /locus_tag="JQG95_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY5890052.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5528..6511)
                     /locus_tag="JQG95_003629"
     CDS             complement(5528..6511)
                     /locus_tag="JQG95_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5890053.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6557..7381)
                     /locus_tag="JQG95_003630"
     CDS             complement(6557..7381)
                     /locus_tag="JQG95_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY5890054.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="JQG95_003631"
     CDS             complement(7464..7718)
                     /gene="yoeB"
                     /locus_tag="JQG95_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY5890055.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1811 g         1829 t
ORIGIN      
        1 tcgaagattg gacggcggat aacattaccc atcttgctat gctctttcgg tacggtccat
       61 acgttgtcca gcaggtcaaa ttctgtcttt gttgccagcc taagctctga gagcctcgcc
      121 ccccacagca taagcatctg atgaaggagc ttatttgacg tagacgcacg gcttctttca
      181 atagcaagcc aaatcttagc cagttcgtga tacgacagta cccgatcccc tacctcagcg
      241 cgggagccga agtcccttgg ctggatgctc ataattgcgc aactatctat caactgacgc
      301 cgcatgcacc aactaattgc tgatcttagt tgacttagca cctgccttgc tcggcgtgga
      361 ttatcttttt cttcttcggt aagcaggtca acccattgct taaccgtgat agaagatgcc
      421 ggacgattag ggaaggcgtc atgcatgcgc ttcataaccg ttgatcgata aagtgcctgg
      481 gtcttttctc tgagagttgt agagacgtag ttgtcgaacc agtagtcgag acactgggcg
      541 accgtcatgg agttctccac cttctcttca aaataggtgc gtggatccgt tcctgagaaa
      601 tagagctttc gcaagtcagc agtgatctgt ctggcatcct tcaaagacag ggatgggtat
      661 cgaccaagcc caagtcgatt aggcttgcca tgccaacgat agcggtactg gaactggatg
      721 acccccttcg gtgaaattcg tacgctgagg ccatcggcat cagccacttc ttgtgggccc
      781 gaatatggtt taccataaat agtacgcagt tttgtgtcgc ttatagccat aaataatatt
      841 tggtacgctc agaaataata gtttggcaca catctggtac acaatatcac atggacaaca
      901 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaagggttgc
      961 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
     1021 acagataaac aacctacctt cctctttcac gattacgaaa cctttggcac gcaccccgcg
     1081 ttagatcgcc ctgcacagtt cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc
     1141 gaacccgaag tcttttactg caagcccgcg gatgactatt taccccggcc tggagcagta
     1201 ttaattaccg gtattacccc gcaggaggca cgggcgaaag gagaaaacga agccgcgttt
     1261 gccgcccgta ttcactcgct ttttaccgta ccgaagacct gtattctggg ctacaacaat
     1321 gtgcgtttcg acgacgaagt cacacgcaac gttttttatc gtaatttcta cgatccttac
     1381 gcctggagct ggcagcatga taactcgcgc tgggatttac tggatgttat gcgtgcctgt
     1441 tatgccctgc gcccggaagg aataaactgg cctgaaaatg atgacggtct accgagcttt
     1501 cgccttgagc atttaaccaa agcgaatggt attgaacata gcaacgccca cgatgcgatg
     1561 gctgatgtgt acgccactat tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg
     1621 tttgattatc tctttaccca tcgtaataaa cacaaactga tggcgttgat tgatgttccg
     1681 cagatgaaac ccctggctca cgtttccgga atgtttgggg catggcgtgg caataccagc
     1741 tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg
     1801 gcaggagaca tttcgccatt actggagctg gatatcgaca cattgcgcga gcgtttatat
     1861 accgcaaaag ccgatcttgg cgataacgcc gccgttccgg ttaagctggt gcatatcaat
     1921 aaatgtccgg tactggccca ggcgaatacg ctacgcccgg aagatgccga ccgactggga
     1981 attaatcgtc agcattgcct cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt
     2041 gaaaaagtgg tagcgatatt cgcggaagcc gaaccgttta caccttcaga taatgtggat
     2101 gcacagcttt ataatggctt tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg
     2161 gaaaccgagc cgcgtaattt accggcactg gatatcactt ttgttgataa acgaattgaa
     2221 aagttgttgt tcaattatcg ggcacgcaac ttcccgggga cgctggatta tgccgagcag
     2281 caacgctggc tggagcaccg ccaccaggtc ttcacgccag agtttttgca gggttatgct
     2341 gatgaattgc agatgctggt acaacaatat gccgatgaca aagagaaagt ggcgctgtta
     2401 aaagcacttt ggcagtacgc ggaagagatc gtctaatttg aagccgccgc cgctggtacg
     2461 gcggaggaca atgttgagtc aggctttttg aacggtgatg ctccacgctg catcgccaat
     2521 ctgctgataa tcggtgatgg catgtccttc ttctgccgcc cactgtggga ttgcttcagt
     2581 tgcctgggtg caatcaaact caattaccaa ctcatcgccg ctggccattt ctgccagtgc
     2641 tgcttttgct tctatgagtg gaaacggaca gacctgcgtc accacatcca gctttttaat
     2701 cgccatcttt acaccttact taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg
     2761 gtcgaacata cacaagccag gacgcagtcc agactccgag aatcataaat acaaggccaa
     2821 tccagccctg ccaggtcatc attgcagtca taaccagtcc attaccgata gagcaaccac
     2881 ctgcaatact ggcaccgaag cccatcagta caccacctaa cccgctacgt aatgttgttt
     2941 gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt ggctgcaata aatgcctcca
     3001 cgaagatccc taacaccagg aaaacgcccc agttaatgta tttcacgtca cccgcgacca
     3061 gaaattgaag aatattggcc gttggggaag tgactcctaa cccaaacatg cgtccggttg
     3121 cttcactcag gggccaggct aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg
     3181 gatgccagcg tttttcaaac agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg
     3241 cgactttgag ttttggcttc ttcaactctt tcatcaccac ccagagcgtt atcaccagca
     3301 aaacggtaac caacggccac acagacaaat taaaagtctc agcaatagag ttatgttcag
     3361 tagtgtagtg ctgcaaggtt tgatttaaac cactggcgtg tggagaacgc atcaccgcac
     3421 tcatcaccat ataagtgaaa agcgcgatcc aactgccgat caatccctca cccgcgcgat
     3481 accaggtccc ggtggcacat ccacccgcca gaacaattcc cagcccgaaa agatacccac
     3541 ctataacagt accaagccac gggaacgccc cggcttcgta agtcagtagg cccgcctgaa
     3601 ttaacgcaaa aacccccacg ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc
     3661 gattattttt cgcgatatac atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct
     3721 gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt gaaaacataa
     3781 attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga atgcagacca
     3841 ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga taaaaaagaa
     3901 taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa agatgattat
     3961 gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca agccaggtaa
     4021 atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc caggttaacc
     4081 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
     4141 ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga caggttaaca
     4201 aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac caggtcgaag
     4261 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
     4321 ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc cattacgtac
     4381 atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt aatggtggag
     4441 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
     4501 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
     4561 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
     4621 cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag
     4681 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
     4741 ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
     4801 cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt gacgttcgct
     4861 acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc caccacaaac
     4921 atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     4981 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     5041 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     5101 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     5161 ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc
     5221 tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     5281 acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta cggtgataac
     5341 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     5401 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     5461 agcaaaaaat aaccgacgct aattaaacgt cggtttttaa tcattttttg acagcggcgc
     5521 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     5581 tttgaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     5641 ctaaaatttc atctcgccac cagccgctgg caagccctgc ggtctgttca ttccagccaa
     5701 tccagacagg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcgccactat
     5761 cgagaaaacg ttgcgccaga taccgtggta aatagccgca gccaaggccg ctaatttgca
     5821 gctccagttt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     5881 aggccgtaga agcgcctgcc tgcgcagtgt cccccaccac aatcgcccga tatcgtttaa
     5941 tgatacgacg gtttaacggt tcttcttcct gggctaacgg atgatgaggc gcgaccgcaa
     6001 agacttgttc aagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     6061 gcatcgcgcc aacgataata tccgctcgcc cctgagtcag ggcatcccag gaaccaccga
     6121 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatga
     6181 gaggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagc tcgttttccc
     6241 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     6301 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     6361 caagcaattg aatatttagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     6421 gcgtcttgta gagtttggct gacgccgccg caaaactgcc ctctttttct atggcatcga
     6481 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     6541 ctgctggcgt gatcgtttac tccaacggca ttaccagcgg atcgggatac tggtattcaa
     6601 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     6661 tatttctgaa ctgcggaggt tgcagcccca gtaaacgggc catctgcgga tagaaaacat
     6721 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     6781 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     6841 catcaggggc ggtttttccg gcaaagaagc gtcccggatg acgtcccggt ccgaccaggc
     6901 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     6961 cttctaatac tcgtccactg ttggttaccg gattacgcgg ggtagtttct ttaaccgtgc
     7021 cttgcgcgtc gccatagaca gatgttgagc tggtaaaaat aattcgggga atacgatggg
     7081 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     7141 tacgacgtgc cggaagcgta atgaccagcg catcggcatc cataagggca tccagatcgt
     7201 cagaatcgca aactaactca ggctccatgc gaagcagata gctatcaatg ccactcattc
     7261 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     7321 aaagtgacat cgccagcggc atacctaacc accctaaccc gacaattgcg acctttttca
     7381 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     7441 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     7501 tcatcagtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     7561 gacaaattat gtttcagggg ttctggtttc cccttacctt caaatggtgt tctgcgggta
     7621 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     7681 taatcatccc atgattcctc agaccagatt agtttcact
//
LOCUS       DADLCQ010000164         7673 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000164 DADLCQ010000000
VERSION     DADLCQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.166"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JQG95_003632"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JQG95_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY5890056.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JQG95_003633"
     CDS             complement(1614..1958)
                     /locus_tag="JQG95_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY5890057.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQG95_003634"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JQG95_003634"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY5890058.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQG95_003635"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JQG95_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY5890059.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JQG95_003636"
     CDS             complement(6144..6842)
                     /locus_tag="JQG95_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY5890060.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQG95_003637"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JQG95_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY5890061.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..>7673
                     /locus_tag="JQG95_003638"
     CDS             7537..>7673
                     /locus_tag="JQG95_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase YfiF"
                     /protein_id="HAY5890062.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DG"
BASE COUNT         1861 a         1958 c         1962 g         1892 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 acgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tgg
//
LOCUS       DADLCQ010000165         7554 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000165 DADLCQ010000000
VERSION     DADLCQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.167"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="JQG95_003639"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="JQG95_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="JQG95_003640"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="JQG95_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY5890063.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="JQG95_003641"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="JQG95_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="JQG95_003642"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="JQG95_003642"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY5890064.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="JQG95_003643"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="JQG95_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY5890065.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /locus_tag="JQG95_003644"
     CDS             complement(5060..6487)
                     /locus_tag="JQG95_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY5890066.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLAYALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="JQG95_003645"
     CDS             complement(6510..7202)
                     /locus_tag="JQG95_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY5890067.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1922 a         1828 c         1987 g         1817 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgactatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ctggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgt aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtat tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagagaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc ccgcgccgct gagaaaaagc gaagcgcact gctctttaac aatt
//
LOCUS       DADLCQ010000166         7460 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000166 DADLCQ010000000
VERSION     DADLCQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.168"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            56..850
                     /locus_tag="JQG95_003646"
     CDS             56..850
                     /locus_tag="JQG95_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY5890068.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="JQG95_003647"
     CDS             872..2281
                     /locus_tag="JQG95_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890069.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="JQG95_003648"
     CDS             2259..3038
                     /locus_tag="JQG95_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY5890070.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3002..3895
                     /locus_tag="JQG95_003649"
     CDS             3002..3895
                     /locus_tag="JQG95_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY5890071.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIG
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="JQG95_003650"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="JQG95_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="JQG95_003651"
     CDS             complement(4635..4895)
                     /locus_tag="JQG95_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY5890072.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="JQG95_003652"
     CDS             complement(4886..6157)
                     /locus_tag="JQG95_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY5890073.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JQG95_003653"
     CDS             complement(6235..6600)
                     /gene="queD"
                     /locus_tag="JQG95_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY5890074.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            6916..>7460
                     /gene="cysJ"
                     /locus_tag="JQG95_003654"
     CDS             6916..>7460
                     /gene="cysJ"
                     /locus_tag="JQG95_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="catalyzes the reduction of sulfite to sulfide in
                     the biosynthesis of L-cysteine from sulfate; a
                     flavoprotein with flavin reductase activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY5890075.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLL"
BASE COUNT         1766 a         1912 c         2005 g         1777 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggcgtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggctgc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacca gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacactccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga
//
LOCUS       DADLCQ010000167         7335 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000167 DADLCQ010000000
VERSION     DADLCQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.169"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            94..1209
                     /gene="adrA"
                     /locus_tag="JQG95_003655"
                     /gene_synonym="dgcC"
     CDS             94..1209
                     /gene="adrA"
                     /locus_tag="JQG95_003655"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY5890076.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1226..2035)
                     /gene="proC"
                     /locus_tag="JQG95_003656"
     CDS             complement(1226..2035)
                     /gene="proC"
                     /locus_tag="JQG95_003656"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY5890077.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2155..2613
                     /locus_tag="JQG95_003657"
     CDS             2155..2613
                     /locus_tag="JQG95_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY5890078.1"
                     /translation="MTIWVDADACPNVIKEILYLAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2796..3320
                     /gene="aroL"
                     /locus_tag="JQG95_003658"
     CDS             2796..3320
                     /gene="aroL"
                     /locus_tag="JQG95_003658"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY5890079.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            3370..3558
                     /gene="yaiA"
                     /locus_tag="JQG95_003659"
     CDS             3370..3558
                     /gene="yaiA"
                     /locus_tag="JQG95_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY5890080.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDK"
     gene            3819..4496
                     /locus_tag="JQG95_003660"
     CDS             3819..4496
                     /locus_tag="JQG95_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY5890081.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4568..4852
                     /gene="ppnP"
                     /locus_tag="JQG95_003661"
     CDS             4568..4852
                     /gene="ppnP"
                     /locus_tag="JQG95_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY5890082.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            5007..5342
                     /locus_tag="JQG95_003662"
                     /pseudo
     CDS             5007..5342
                     /locus_tag="JQG95_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            5339..5794
                     /locus_tag="JQG95_003663"
     CDS             5339..5794
                     /locus_tag="JQG95_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890083.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="JQG95_003664"
     CDS             complement(5872..6783)
                     /gene="rdgC"
                     /locus_tag="JQG95_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY5890084.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            6770..>7335
                     /locus_tag="JQG95_003665"
     CDS             6770..>7335
                     /locus_tag="JQG95_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY5890085.1"
                     /translation="MNHSMIISTALSLNLAGMITNCRRPCIANPVVRLYAIDIEKNKE
                     STVRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQ
                     RGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEA
                     VDGAAAGAQTVFAVTIGTGCGAGVAFNGR"
BASE COUNT         1790 a         1749 c         1922 g         1874 t
ORIGIN      
        1 agtgctaacg ggtgagctac gaaagtggct cacccgaaat ttcatacttc tgcctttagc
       61 cccgtctcta taatttggga aaattgtttc tgaatgttcc caaaaataat gaatgatgaa
      121 aactttttca aaaaagcggc ggcgcatggg gaggaacctc ctttaactcc tcaaaacgaa
      181 catcagcggt ccgggctgcg cttcgcccgt cgcgtcagac taccccgtgc ggttggcctg
      241 gctggcatgt tcttaccgat tgcttcaacg ctggtttcgc acccgccgcc gggctggtgg
      301 tggctggtgt tggtcggctg ggcgttcgtc tggccgcatt tagcctggca gatagcgagc
      361 agggccgtcg atccgcttag ccgggaaatt tacaacttaa aaaccgatgc agtattagcg
      421 ggaatgtggg taggcgtaat gggcgtaaac gtgctgcctt ccaccgctat gttgatgatt
      481 ctgtgtctga atttgatggg ggcaggcggc ccccgtctgt ttgtcgcggg tctggtgttg
      541 atggtggttt cctgccttgt caccctcgag cttacgggca ttaccgtgtc gttcaatagt
      601 gctccgctgg aatggtggct ctcccttccc attattgtcg tttatcctct gatgtttggc
      661 tgggtcagct accagacggc aaccaaactg gcggaacata aacgcaggtt gcaggtcatg
      721 agtactcgcg acggcatgac gggcgtgtat aaccgacgtc attgggaaac tatgttacgc
      781 aatgaatttg ataactgtcg gcggcataat cgcgatgcaa cgttactgat tatcgatatc
      841 gaccatttca agagcatcaa cgatacctgg ggtcatgatg tgggcgatga agcgattgtg
      901 gcgcttaccc gacagttaca aattaccctg cgcggtagcg atgtgattgg tcggtttggc
      961 ggcgatgagt ttgcagtaat catgtccggt acgccagctg agagcgccat taccgccatg
     1021 ttacgggtgc gtgaagggct aaacacgtta cgcttgccga atacgccaca ggtaacttta
     1081 cggattagtg tgggggttgc gccgctgaac ccacaaatga gtcactatcg tgagtggttg
     1141 aaatcggcag atttggcgct ttacaaagca aagaaagccg gacgtaaccg caccgaagtg
     1201 gcggcctgac gtccggcgaa agtcatcagg atttgctgag tttttctgat ttttccatac
     1261 acttcgtcat cgcttcgatc actgcggcac ggaagccttt ctcttccagt acgcgtaccg
     1321 cttcaatggt ggtgcctccc ggtgagcaga ccatatcttt cagtgccccc ggatgttctc
     1381 ccgtttccag caccattttt gcggaaccca ttaccgcctg agcggcaaat ttatacgcct
     1441 gggcgcgtgg catcccgccc agcacggcgg cgtcggccat cgcttcgata aacataaata
     1501 cgtaggctgg cgaagaaccg ctcacaccga ccaccgggtg gatcatcggc tcagcaatta
     1561 cttccgcttc gccaaagcag cggaaaatat tcagcacatc agcggtatct tctggggtta
     1621 ccagcgcgtt tggcgttacg gaggtcatcc cggcattaac cagcgcggga gtgttcggca
     1681 tggcgcggat aattttccgg tcatggccca gcgcgcgggc aagctggtcg agcgtgacac
     1741 ctgcagcaat agaaacgacc agagagtctt tattcaggct ggaggtgatt tcgctaagca
     1801 ctttaatcat gatgccaggt ttaacggcag caaaaatgat gtcggcgatt tgcgccactt
     1861 cttgcgccga ttctgcggcg ttgatgccga actggtcatg cagggcggcg actttatccg
     1921 gggagggggt gtatacccag atttgccctg gaagcacctg accgctggca atcagaccgc
     1981 cgagaatggc ttttcccata ttgccgcagc caataaaacc gattttcttt tccattgcct
     2041 cactcctgcc gtgaaattca ttgttttgat aatcgctggc agaagcataa acagaactat
     2101 gccggaaggc aaaagcgcga cacaatagag gattacctaa caaaggatga ctttatgaca
     2161 atttgggtgg atgccgacgc gtgtcccaat gtaattaaag agattttgta tctcgcggcg
     2221 gaacgtatgc agatgccgct ggtactggta gcaaaccaga gtttacgcgt gccgccatcg
     2281 cgatttattc gtacgctgcg cgtcgcggca ggtttcgacg ttgccgataa cgaaattgtc
     2341 cggcagtgtg aagcgggcga tttggtgatc accgcagata tacctttggc tgctgaagcc
     2401 atcgagaaag gcgcagcggc gcttaattcg cgcggcgaac gttacacgcc agcgaccatt
     2461 cgtgagcgcc tgacgatgcg cgattttatg gataccttac gtgccagtgg gatccagacc
     2521 ggcggaccag atagcctttc acaacgtgac cgccaggcct ttgccgcgga gctggagaag
     2581 tggtggctgg aagtgcaacg tagtcgtggc taaatgtaat ttattattta cacttcattc
     2641 ttgaatattt attggtatag taagggatgt attgagattt ttactttaag tggaattttt
     2701 tctttacaat cgaaattgta ctagtttgat ggtatgatcg ctattctcat gacaccggct
     2761 ttcgccgcat tgcgacctat tggggaaaac ccacgatgac acaacctctt tttctgatcg
     2821 ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct tgccgattcg cttaaccgtc
     2881 ggtttgtcga taccgatcag tggttgcaat cacagctcaa tatgacggtc gcggagatcg
     2941 tcgaaaggga agagtgggcg ggatttcgcg ccagagaaac ggcggcgctg gaagcggtaa
     3001 ctgcggcatc caccgttatc gctacaggcg gcggcattat tctgacggaa tttaatcgtc
     3061 acttcatgca aaataacggg atcgtggttt atttgtgtgc gccagtatca gtcctggtta
     3121 accgactgca agctgcaccg gaagaagatt tacggccaac cttaacggga aaaccgctga
     3181 gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct atatcgcgaa gttgcgcata
     3241 ttatcatcga cgcaacaaac gaacccagcc aggtgatttc tgaaattcgt agcgccctgg
     3301 cacagacgat caattgttga ttttagagcg cctatactta acgttcatcc cgtgaaataa
     3361 ggaagaacga tgccaacgaa accgccttat cctcgtgaag catatatagt gacgattgaa
     3421 aaaggaaagc caggacagac ggtaacctgg taccaactca gagccgatca tcctaaacca
     3481 gactcgttga tcagtgaaca tccgacggct caggaagcga tggatgcgaa aaaacgctat
     3541 gaggaccctg acaaatagtg accgcatcag actgctcgga agggattctg agtgccacta
     3601 caagggatct gcgtcacatt tttcataatt tatgtttttc taataattag aatattaaac
     3661 aataacattc cattactgga atcatttgga atctttacat tatgccgtgc acgtctgctg
     3721 ctacgctttt tgtcatttgt agcacaagta agtgtcagca gtggtgcttc acacttgccc
     3781 ggtaattaac gacgaaagaa aagtaaggtg gatgaacaat gagtgcgtcg ttggcgatcc
     3841 tgaccatcgg cattgtacct atgcaggagg ttttgccgct cctgacggaa tacattgacg
     3901 aagataatat ttcccatcat agcctgctgg ggaagttaag tcgtgaagaa gtgatggcgg
     3961 agtacgcgcc agaagcaggc gaagacacca ttctcacatt attaaatgac aaccagctgg
     4021 cccatgtttc gcgtcgtaaa gtggagcgag atctgcaagg tgtggttgaa gtgctcgata
     4081 atcagggtta tgacgtcatt ttattaatga gtacagcaaa cattagtagt atgactgcgc
     4141 gtaatacgat ctttcttgag ccgtcgcgaa tattgcctcc actggtttcc tctattgttg
     4201 aagatcatca ggtgggggtt atcgttccgg ttgaggagat gctgcccgtt caggcgcaaa
     4261 aatggcaaat tttgcagaaa tcgccggtat tttcattggg taaccccatt catgattcag
     4321 aacaaaaaat cattgatgct gggaaagaat tactggcaaa aggggctgat gtcatcatgc
     4381 tggattgttt aggatttcac caacgtcatc gcgatttact gcaaaaacag ctcgatgttc
     4441 ctgtcttgct ctctaacgta ttgattgcac ggctggctgc ggaattactg gtgtaatttt
     4501 gcgtgacagc cagcgcctct ggcccctata gtgaagtaga tgttcaacta ccagacaggg
     4561 ccagtttatg cttcaaagta atgagtactt ttccggcaaa gtgaaatcga tcggcttttc
     4621 cagcagcagc actggtcgcg ccagcgtggg tgttatggtt gaaggcgaat acaccttcag
     4681 caccgctgag ccggaagaga tgacggtaat cagtggcgcg ctgaatgtgt tactgcctga
     4741 cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt aatgttcccg gtcacagtga
     4801 gtttcatctt caagttgccg aacctacctc ttatctgtgc cgctatctgt aattcctcgc
     4861 cttccccttg aacgggaggg catttttctg aaatatcctc tctttaaccc ataataatat
     4921 ttcctttgct gcgatttttt caatttccga tatattcata attcatcaag gttgatataa
     4981 atatcagtga agatctccag gtattttttg cggaactggc tacgataaaa gataaatcag
     5041 atgatgaatg gtggcgtgca ttgctgcaaa gtcgcaatga tcatttgcgc caaacggcat
     5101 tacgcaacgc acataccccg gcgtcgttgt taacgacatt gactgagcct caggatcggt
     5161 cgctggctat taataatcca cagctggctg ccgatgtgaa aacggcgtgg ttaaaagagg
     5221 atccatcatt actcttattt gtcgaataac ccgatctttc gcagttacgt gatttagtga
     5281 aaaccggggc aacgcggaaa attcgcagtg aagcgcgtca ccggcttgag gaaaaacaat
     5341 gacacaacgt ccatggagca agttacagcg ggagatctat gacttactga cgccaacgat
     5401 caacctgcaa attcactgta cccgttatcc catgcgcagc caaaatggtg gaagtaccga
     5461 tttaccgcgt tattggatca cgttggataa aaacgtgatc tgggattacc cgaaagactt
     5521 tattgcaggt aacgctggag tgcgtaattt tcacggtgaa acttgctggt atccttacct
     5581 gacggatatt tgttcaattt ctgatttatt gcgtgaatat attgataccc caaaagcgga
     5641 gctgttaaca aaacaattta ccagcgataa atgggggtta gtgaatattc tgcgtgctgc
     5701 tgacaggcga atagggatga ggcgattaga tcagttacgg cgtaaaacgc ataatattgc
     5761 tgcgctaaaa attattgctc gccgtagcga ataattatat gcttggtgtg gcttcgtacg
     5821 ccggataaga cgcggcaggc gtcgcatccg gcattaaagg aaaatcagca attaacgttg
     5881 tgcttcgcca cctaatcctt caatcaggtt ttgaattaac gctgccagtt caccagtcat
     5941 caggatgaaa tcggcatcaa aacgctgggc gaaatcttca cggtcgatat cttcgttttg
     6001 atcgcgcagc tcgtcgcaga acttaagacg cttgagcgaa ccatcgtcgc acatcacaaa
     6061 ctgaatgcgc tgctgccagt cgagcgccag tttagtcacc acttttccgg cttcaatgtg
     6121 attggtgatc tcttcgctgg tcagatcttg tttcttcgcg cggatcacgc cgccatcttc
     6181 cagcaacgat ttcagctcgg cttcatcaag cagctggaag ccctgtgccg cactaccgga
     6241 gcgaacccat tcggtcagcg tcagttcaat cgggttttcc atgctcaatg gtacaaccgg
     6301 taacgacccc aggcttttac gcagtaatgc cagcgtatct tccgcttttt tggcactggc
     6361 gcagtccacc ataatcaaac cgttaaccgt gtcgagccac atcattgtct ggctaaaacg
     6421 gctgaaagca cgcggcagca gagagtgcag cacttcgtct ttcagcgaat ctttttcggt
     6481 tttcttcagc ttacgcgcct gttccgcttc cagtttggcg attttcgctt ccagcgcctg
     6541 tttaatcacc ggagacggga ggattttttc ttctttgcgc gcgcagataa caatttgacc
     6601 attggcaacg tgcgttaacg catcgctgtg cgatcccatc ggaggaaccc agcccatctt
     6661 cgccatgtcc tggctgccgc atggggtaaa tgccatcgag gctagctgtt tttccatctc
     6721 ttctgcacgc agcgaaatct cgcggctaag acggtaaacc attaaatttt tgaaccacag
     6781 catgataatt tccacggcct tgtcgttaaa tttagcgggc atgataacga attgtcggcg
     6841 gccttgcatt gccaatccgg ttgtccgtct ctacgctatt gatattgaaa aaaataagga
     6901 gagtacagtg cgtataggta tcgatttagg cggcaccaaa actgaagtga ttgcactggg
     6961 cgatgcaggg gagcagttgt accgccatcg tctgcccacg ccgcgtgatg attaccggca
     7021 gactattgaa acgatcgcca cgttggttga tatggcggag caggcgacag ggcagcgcgg
     7081 aacggtaggt atgggcattc caggttcgat ttcgccctac accggtgtgg tgaagaatgt
     7141 caattcaacc tggctcaacg gtcagccatt cgataaagac ttaagcgcga ggttgcagcg
     7201 ggaagtgcgg ctggcaaatg atgccaactg tctggcggtt tcagaagcag tagatggcgc
     7261 ggcagcggga gcgcagacgg tatttgccgt gactatcggc acgggatgcg gcgcgggcgt
     7321 ggcattcaat gggcg
//
LOCUS       DADLCQ010000168         7183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000168 DADLCQ010000000
VERSION     DADLCQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.170"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(296..1198)
                     /locus_tag="JQG95_003666"
     CDS             complement(296..1198)
                     /locus_tag="JQG95_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY5890086.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQG95_003667"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JQG95_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY5890087.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JQG95_003668"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JQG95_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY5890088.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     DLSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JQG95_003669"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JQG95_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY5890089.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAFIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JQG95_003670"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JQG95_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY5890090.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JQG95_003671"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JQG95_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1673 a         1811 c         1878 g         1821 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatgcccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgacttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc cactctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg ttcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgctctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DADLCQ010000169         6920 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000169 DADLCQ010000000
VERSION     DADLCQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.171"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(194..1036)
                     /gene="purU"
                     /locus_tag="JQG95_003672"
     CDS             complement(194..1036)
                     /gene="purU"
                     /locus_tag="JQG95_003672"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY5890091.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1086..1544)
                     /locus_tag="JQG95_003673"
     CDS             complement(1086..1544)
                     /locus_tag="JQG95_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY5890092.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1657..2562
                     /gene="rssA"
                     /locus_tag="JQG95_003674"
     CDS             1657..2562
                     /gene="rssA"
                     /locus_tag="JQG95_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY5890093.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            2654..3667
                     /gene="rssB"
                     /locus_tag="JQG95_003675"
     CDS             2654..3667
                     /gene="rssB"
                     /locus_tag="JQG95_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY5890094.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3869..4777
                     /gene="galU"
                     /locus_tag="JQG95_003676"
     CDS             3869..4777
                     /gene="galU"
                     /locus_tag="JQG95_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY5890095.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4921..5334)
                     /gene="hns"
                     /locus_tag="JQG95_003677"
     CDS             complement(4921..5334)
                     /gene="hns"
                     /locus_tag="JQG95_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY5890096.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5939..6556
                     /gene="tdk"
                     /locus_tag="JQG95_003678"
     CDS             5939..6556
                     /gene="tdk"
                     /locus_tag="JQG95_003678"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY5890097.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
BASE COUNT         1824 a         1517 c         1692 g         1887 t
ORIGIN      
        1 agtctgctcc ctttggccgc tcgggaaccc caccacgggg taatgctttt tactagcctg
       61 ctcccttatc gggaagcggg gcgcatcata tcaaatgacg tgccgctgta aagtgttacg
      121 ttgagaaaaa tgaactggtt gcgtaatttt catccgtaac ggattaaagg taaccagtta
      181 tttttgctgg cgattaaaga ataatcgtcc gattaccgta aacaaagacg cgttgcgcca
      241 gtactttgta tagcgcacga cttaagacgt ttttctcgac gtcacgacct gcgcgcatca
      301 tatcttcagc tgtgtaggta tgatcgacat gaataacgtc ctgcatgatg attgggcctt
      361 cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat aatcttcaca ccacgttcat
      421 aggcctggtg ataaggacgt gcgccgataa acgcaggcag gaatgaatgg tgaatattga
      481 tgattttatt cgggaagcgt gccacaaatt ccggcgttaa tacccgcata tacttcgcca
      541 gcaccacata gtcaggttga taagcatcaa tggcgtccgc catcttttga tcgtgctcgt
      601 tgcggcttaa cccttcatgg cttaccagct caaacggaat atcaaaacgc tcaaccagag
      661 aacgtaaagt atcgtggtta ccaataactg ccgcgatttc gacatccaga ccgccgtaat
      721 tggctttcat caacaaatcg ccaaggcaat gcgcttcttt agtgaccaga atcactatcc
      781 ggcgacgacc ggcaggattc agctcacgca cggagccttc tggcaatgcg ctatcgagat
      841 ccgccagcag ggtggaatca ttaaaaatcc cttccagttc cgtgcgcata aaaaagcgcc
      901 cggtacggtg atcaacaaat tcattgttct gtacgatatt taactcgtgc ttgtagcaaa
      961 tattggtaat acgtgcgatc agaccttttt ggtccggaca aatagtacgc agaactttac
     1021 gttggagtga atgcattgct ggaaaaacct tgttgagagt gtttgctaaa ccgtaccgtc
     1081 aaccattatt ggccgcagca ctttttaaat tttttacctg aaccacaagg gcagggatcg
     1141 ttgcgaccaa actgcggacg tgtaccgtca atatagtacc attgaccgtt ttctttcaaa
     1201 aatcgcgaac gttcaattat tgcacctgtt ttgccacctt cggtaaatcg agcaacaaaa
     1261 ctgacaaatc cgatattgtc ggcgtcctgc caacaatgtt caaatacggt caatcccaac
     1321 cattcagtat gcgcaaatcc ggtcatcaat tcggcacgta acgccgctgc tccacaagag
     1381 ggatgccagg tctttattaa ataatcagcg tcttgcatca caaaagcgca gtaacgcgaa
     1441 cgcatgagat gttctggatc aggtgcaacc ttttcaccag acacataagg gtggcaacat
     1501 aggctatact cgacagcact accacaggga caaagctgag acacaaataa tctccctgga
     1561 aacaataacg gcgtattaac cgcctgagta gcactatgtt aaccgagcag tagcgatgtg
     1621 gctacgattg cattccaggg gaatcttgcg ggaataatga gaaagataaa aatagggctg
     1681 gcgctgggat ctggcgcggc gagaggttgg tcgcatattg gcgttattaa tgcgctaaaa
     1741 aaagtgggta ttgaaattga tatcgttgca ggatgttcaa ttggttcgct ggtgggcgcg
     1801 gcctatgcat gcgatcgatt atctgcgctg gaagattggg taacctcttt cagttattgg
     1861 gacgttttac gcctgatgga cctctcctgg cagcgcggtg ggttactgcg cggcgagcgt
     1921 gtcttcaatc aatatcgcga aataatgccg gaaacagaga tcgaaaattg ttcccgtcgc
     1981 tttgcggctg ttgccaccaa tttaagtacg gggcgtgaat tatggtttac tgaaggcgat
     2041 ctccatcttg ctattcgcgc atcatgcagt attccaggac tcatggcacc cgttgcacat
     2101 aacggctact ggctggttga tggggcagta gttaacccaa ttcctatttc tcttacgcgt
     2161 gcattggggg ctgatattgt gatagcggtc gacctgcagc acgatgctca tttgatgcaa
     2221 caagatctgc tctcctttaa tgtcagtgaa gaaaatagcg agaatggtga ttctctaccg
     2281 tggcatgcgc gtctgaaaga aaggttaggc agcataacga cacgtcgggc tgtgacagcg
     2341 ccaacggcaa cagagattat gaccacgtct atccaggtgt tggagaaccg ccttaaaagg
     2401 aaccgcatgg caggtgatcc gcccgatatt ctgattcaac ctgtttgccc gcaaatatct
     2461 acgcttgatt tccatcgcgc gcacgctgcc attgcggccg gacagctggc agtggaaagg
     2521 aaaatggacg aacttttgcc gttggtacgc accaacattt gaccagaatt tttatctaca
     2581 cttaagttaa ttctgacagg cgcaggtggc aatagcatgc cactattgag taaagccagt
     2641 caggggagag aacatgacgc agccattggt cggaaaacag attctcattg ttgaagatga
     2701 gcaggtattt cgctcgcttc tggattcatg gttttcctca ttgggagcga caacggtact
     2761 ggcggctgat ggggtggatg cccttgagtt gctgggaggt ttcactccag acctgatgat
     2821 atgtgatatc gcgatgccac gaatgaacgg gcttaaactg ctggagcata tacgtaacag
     2881 aggcgaccag accccagttc tggtgatatc tgccactgaa aatatggcag atattgccaa
     2941 agcgttacgt ctgggcgttg aagatgtttt gctgaaacca gttaaagatc tgaatcgctt
     3001 gcgcgagatg gtttttgcct gtctctatcc cagcatgttt aattcgcgcg ttgaggaaga
     3061 ggaaaggctt tttcgcgact gggatgcaat ggttgataac cctgccgcag cggcgaaatt
     3121 attacaggaa ctacaaccgc cggttcagca ggtgatttcc cattgccggg ttaattaccg
     3181 tcaattggtt gccgcggaca aacccggcct ggtgcttgat attgccgcac tttcggaaaa
     3241 cgatctggca ttttattgcc ttgatgtcac ccgagctgga cataatggcg tacttgctgc
     3301 cttgttatta cgcgcattgt ttaacggatt attacaggaa cagcttgcac accaaaatca
     3361 acggttgcca gagttgggcg cgttattgaa gcaggtaaac catttacttc gtcaggccaa
     3421 tctgccgggg cagtttccgc tattagttgg ctattatcat cgcgaactga aaaatctcat
     3481 tctggtttct gcgggtctga atgcgacgtt aaataccggc gaacaccagg tgcaaatcag
     3541 taatggtgtt ccgttaggca ctttaggtaa cgcttatttg aatcaattga gccagcgatg
     3601 cgatgcctgg caatgccaaa tatggggaac cggtggtcga ctgcgcttga tgttgtctgc
     3661 agaatgagca aacgataacg cgggctaaat ttgcattacc tgctaatgtc ggctggtggt
     3721 actatcgtcg ccattcgtat aagtaattgt cttaattatg ctatctcgcc tccttttcag
     3781 aacttagccc cttcggggtg ctgatatact gggatgcgat acagaaatat gaacacgttc
     3841 aaaacacgaa cagtccagga gaatttaaat ggctgccatt aatacgaaag tcaaaaaagc
     3901 cgttatcccc gttgcgggat taggaaccag gatgttgccg gcgacgaaag ccatcccgaa
     3961 agagatgctg ccacttgtcg ataagccatt aattcaatac gtcgtgaatg aatgtattgc
     4021 ggctggcatt actgaaattg tgctggttac acactcatct aaaaactcta ttgaaaacca
     4081 ctttgatacc agttttgaac tggaagcaat gctggaaaaa cgtgtaaaac gtcaactgct
     4141 tgatgaagtg cagtctattt gtccaccgca cgtgactatt atgcaagttc gtcagggtct
     4201 ggcgaaaggc ctgggacacg cggtattgtg tgctcacccg gtagtgggtg atgaaccggt
     4261 agctgttatt ttgcctgatg ttattctgga tgaatatgaa tccgatttgt cacaggataa
     4321 cctggcagag atgatccgcc gctttgatga aacgggtcat agccagatca tggttgaacc
     4381 ggttgctgat gtgaccgcat atggcgttgt ggattgcaaa ggcgttgaat tagcgccggg
     4441 tgaaagcgta ccgatggttg gtgtggtaga aaaaccgaaa gcggatgttg cgccgtctaa
     4501 tctcgctatt gtgggtcgtt acgtacttag cgcggatatt tggccgttgc tggcaaaaac
     4561 ccctccggga gctggtgatg aaattcagct caccgacgca attgatatgc tgatcgaaaa
     4621 agaaacggtg gaagcctatc atatgaaagg gaagagccat gactgcggta ataaattagg
     4681 ttacatgcag gccttcgttg aatacggtat tcgtcataac acccttggca cggaatttaa
     4741 agcctggctt gaagaagaga tgggcattaa gaagtaacat ccgtatcggt gttatccacg
     4801 aaacggcgtt gagcaatcga cgccgttttt ttatagctta ttcttattaa attgtcttaa
     4861 accggacaat aaaaaatccc gccgctggcg ggattttaag caagtgcaat ctacaaaaga
     4921 ttattgcttg atcaggaaat cgtcgaggga tttaccttgc tcatccattg cttttttgat
     4981 tacagctgga gtacggcctt ggccagtcca ggttttagtt tcgccgtttt cgtcaacgta
     5041 gctatatttt gccggacgct gagcacgttt agctttggtg ccagatttaa cggcagcaag
     5101 gctattcagc agttcgttcg ggtcaatacc gtcagcgatc agcatttcgc gatattgctg
     5161 cagcttacga gtgcgctctt caacttcagc agcagccgcg ctttcttctt cgcgacgttc
     5221 gttaacaaca acttctaatt tttccagcat ttcttccagc gtttcaagtg tacattctct
     5281 tgcctgcgca cgaagagtac ggatgttgtt cagaatttta agtgcttcgc tcattgtagt
     5341 aatctcaaac ttatattggg gtggtttgtt gaggtaataa tagagcctta aattcagttg
     5401 tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt attttgtgcc gccaataaat
     5461 atcttttcat aaaattagcc agaaaagacg cggcatatag ccctatttac accgatgatt
     5521 tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca ttttatgggg agattatcgt
     5581 aggctgacga cctttcagtc ttctgtatta gttgtgttta cgagaattcc ctattaagcg
     5641 aatgatgaaa agtagaacag tcgcaataag agcatggact tagtattgca ctatctcctg
     5701 gaggtcaaca gagggctatt acttgcgcaa caggttaaag attgtgaata gttaccagca
     5761 gtcatttacc cgcttataac aagcgaggca gttgtaatga tagctcagaa ggattatgca
     5821 aggcttcgta agggagaacg catataccca cttctgtgca tactgttgag ctgaaaaact
     5881 gacgaattat gataaactcc agccaacttt atttcatatc attgagggcc tgtggctgat
     5941 ggcacagcta tatttctact attccgcaat gaatgcgggt aagtctacag cattgttgca
     6001 atcttcatac aattaccagg aacgcggcat gcgcactgtc gtatatacgg cagaaattga
     6061 tgatcgcttt ggtgccggga aagtcagttc gcgtataggt ttgtcatcgc ctgcaaaatt
     6121 atttaaccaa aattcatcat tatttgatga gattcgtgcg gaacatgaac agcaggcaat
     6181 tcattgcgta ctggttgatg aatgccagtt tttaaccaga caacaagtat atgaattatc
     6241 ggaggttgtc gatcaactcg atatacccgt actttgttat ggtttacgta ccgattttcg
     6301 aggtgaatta tttattggca gccaatactt actggcatgg tccgacaaac tggttgaatt
     6361 aaaaaccatc tgtttttgtg gccgtaaagc aagcatggtg ctgcgtcttg atcaagcagg
     6421 cagaccttat aacgaaggtg agcaggtggt aattggtggt aatgaacgat acgtttctgt
     6481 atgccgtaaa cactataaag aggcgttaca agtcggctca ttaacggcta ttcaggaaag
     6541 gcatcgccac gattaataag aatttcttta ctgacagggt gagcagggca cttttatcct
     6601 gtcagttcgt tttacgcact tcttccgggc tatataccct tctcggcagt tttttaacgc
     6661 cgctatacgc ctcacagggc tcttaagcac cgacgttgac ttgtgacctg taaagtacaa
     6721 tatccctgtg tttaggcgtt atacatcgtc gcaaatatga tgaaggctaa tgctgtcggt
     6781 ttatggaaaa gttgctttgg gtaaacaaaa aaacggcccc agaaggggta atgccagtca
     6841 gttaagcaac tgactggctc tttttcggag ctgtggggta tttccagggc ctctccttta
     6901 ccactctcgg gaaggccctt
//
LOCUS       DADLCQ010000170         6824 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000170 DADLCQ010000000
VERSION     DADLCQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.172"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..236
                     /locus_tag="JQG95_003679"
     CDS             <1..236
                     /locus_tag="JQG95_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890098.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JQG95_003680"
                     /pseudo
     CDS             276..816
                     /locus_tag="JQG95_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /locus_tag="JQG95_003681"
     CDS             883..2301
                     /locus_tag="JQG95_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY5890099.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /locus_tag="JQG95_003682"
     CDS             2282..2752
                     /locus_tag="JQG95_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY5890100.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JQG95_003683"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JQG95_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY5890101.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JQG95_003684"
     CDS             3834..4751
                     /locus_tag="JQG95_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY5890102.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JQG95_003685"
     CDS             4830..5012
                     /locus_tag="JQG95_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY5890103.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            5183..6824
                     /gene="yedQ"
                     /locus_tag="JQG95_003686"
                     /pseudo
     CDS             5183..6824
                     /gene="yedQ"
                     /locus_tag="JQG95_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
BASE COUNT         1642 a         1653 c         1921 g         1608 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctcggtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgcctgg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcaacaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tctagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcgg atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcggtacg ctattgccgg gttatgccag aatcataaaa
     5161 aagcaggttg ggagtcgtca gggtgcagca cgagacaaaa atggaaaacc agagctggtt
     5221 gaaaaaactc gcacgccgcc tggggcccgg tcatgtcgtt aatctctgct ttatcgtggt
     5281 attgcttttt tccaccttgc tcacctggcg tgaagtggtg gtgctggaag atgcctatat
     5341 ctccagctag cgtaatcatc tggaaaacgt agccaacgcg ctcgataagc atttgcagta
     5401 taacgtcgac aaactgatct ttttgcgtaa tggcatgcgc gaagctctcg tggcgccact
     5461 ggatttcacc tctctgcgta atgccgtaac cgagttcgaa cagcatcgcg acgagcacgc
     5521 ctggcaaatc gaactcaacc gacggcgcac cctgccagtc aatggcgtat cggatgcatt
     5581 agtcagcgag gggaatttcc tgtctcgcga aaatgaaagc ctcgacaatg aaattaccgc
     5641 tgcactggaa gttggttact tgctgcgact ggcgcacaac tcctcgtcga tggttgaaca
     5701 ggcgatgtat gtctcgcgtg ctggatttta cgtttcgacg cagccgacct tgtttacgcg
     5761 caatgtacca acgcgttatt acggctatgt cacccaaccc tggtttatcg gccattcgca
     5821 acgagaaaat cgtcaccgcg cggtacgctg gttcacttcg caaccggaac acgccagcaa
     5881 tactgaaccg caggttaccg tcagtgttcc ggtagacagt aataactact ggtatggcgt
     5941 gctggggatg agtattcccg tgcgtactat gcagcaattt ttaagaaacg ccatcgataa
     6001 aaacctcgat ggtgagtatc agctctatga cagtaagctg agatttttga cctcttccaa
     6061 tcctgaccat ccaacaggga atatttttga tcctcgtgaa ctggccttgc tggcgcaggc
     6121 aatggaacat gacacgcggg gcggcattcg tatggacagt cgctatgtta gctgggaacg
     6181 tctggaccat ttcgacggtg tgctggcgcg cgtccatacg ctaagcgaag gcgtgcgcgg
     6241 cgatttcggc agtatcagca ttgcattaac cctgctgtgg gcgctcttta ccaccatgtt
     6301 actcctctcc tggtatgtga ttcgccggat ggtcagcaac atgtatgttc tgcaaagctc
     6361 gttgcagtgg caggcgtggc acgacacctt aacgcgttta tataaccgtg gcgcactgtt
     6421 cgaaaaagcc cgtccgctcg ctaaattgtg tcagacgcac caacatcctt tttctgtcat
     6481 tcaggtcgat cttgaccatt ttaaagcgat taatgaccgc tttggtcatc aggcgggcga
     6541 ccgtgttctt tctcatgctg ccggattaat tagcagttcc ttgcgtgcgc aggacgttgc
     6601 cgggcgggtc ggtggtgagg agttttgtgt gattctgcca ggcgcgaatc tgacgcaggc
     6661 tgcggaggtc gcagaacgta ttcgcctgaa gttaaatgaa aaagagatgc tgatcgccaa
     6721 gagtacaacg atacgcatca gtgcctcgct gggggtaagt agcagcgagg aaaccggtga
     6781 ttatgatttt gaacaactcc agtcattggc cgaccgtcgg cttt
//
LOCUS       DADLCQ010000171         6690 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000171 DADLCQ010000000
VERSION     DADLCQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.173"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(220..600)
                     /locus_tag="JQG95_003687"
     CDS             complement(220..600)
                     /locus_tag="JQG95_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY5890104.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(593..793)
                     /locus_tag="JQG95_003688"
     CDS             complement(593..793)
                     /locus_tag="JQG95_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY5890105.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(888..1145)
                     /locus_tag="JQG95_003689"
     CDS             complement(888..1145)
                     /locus_tag="JQG95_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890106.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(1142..2533)
                     /locus_tag="JQG95_003690"
     CDS             complement(1142..2533)
                     /locus_tag="JQG95_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY5890107.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(2530..3408)
                     /locus_tag="JQG95_003691"
     CDS             complement(2530..3408)
                     /locus_tag="JQG95_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5890108.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(3419..4327)
                     /locus_tag="JQG95_003692"
     CDS             complement(3419..4327)
                     /locus_tag="JQG95_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5890109.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETPRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSSRQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARL
                     REGIKPEAMLDGVKRYAAWVRATGNTGTQFVKQAATFFGPDRHFEDFWQQPAAPRGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(4314..4547)
                     /locus_tag="JQG95_003693"
     CDS             complement(4314..4547)
                     /locus_tag="JQG95_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890110.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(4544..5560)
                     /locus_tag="JQG95_003694"
                     /pseudo
     CDS             complement(4544..5560)
                     /locus_tag="JQG95_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114998.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5492..5755
                     /locus_tag="JQG95_003695"
     CDS             5492..5755
                     /locus_tag="JQG95_003695"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY5890111.1"
                     /translation="MRARYQHLVERMKVYGSNLGEPHTSAFGDGLFELRIKGNDGIAR
                     VFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW"
     gene            5745..6053
                     /locus_tag="JQG95_003696"
     CDS             5745..6053
                     /locus_tag="JQG95_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5890112.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(6095..6271)
                     /locus_tag="JQG95_003697"
     CDS             complement(6095..6271)
                     /locus_tag="JQG95_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY5890113.1"
                     /translation="MRRHRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTT
                     HLWDIRWLRHRARK"
     gene            complement(6268..6678)
                     /locus_tag="JQG95_003698"
     CDS             complement(6268..6678)
                     /locus_tag="JQG95_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY5890114.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
BASE COUNT         1611 a         1799 c         1555 g         1725 t
ORIGIN      
        1 accaactgag ctaaagggcc ggaggcagaa taataaccat atgtcatcac atctgcaaac
       61 tcatctgacc accagcgtgt ttaacgtcct gtaccgtttt tcaggcataa aaaacccgcc
      121 cggaggcggg tttaagctgt gtggcgaagc aaccactctt aacagaatat cccgtttttt
      181 acgtacgtaa aatattttct agaaagtcgc cccttaccat cagggatgtt caatatattt
      241 gtccatttct aaccggacac ccaacatcat caacattcct tccaccaccc cctcggcttt
      301 ttgtagcttc tttcctatat aaccatctga gcatccatgc ttccgcgcca gcgtcatgaa
      361 tgtcatccca aacacgtagt agtcaaccag caagtcatgt agatcgttgt tgttccggtt
      421 aaggcgggcc atacacccac atataaccat cgcgtcatcg tcacagcatt gcgggcggga
      481 ttttactttt gaagggatta atcccttaaa accggcagca atggacgacc aggtcacatc
      541 ctcatgatta ttagccaccc acgcgcccca gcgctcaaga acctgctgaa tatcacgcat
      601 cactgtcttt acccctgtcc catccacgat gaacaatcag aacaccatca acaatggcgt
      661 gccctttgcc ctctttatct tcagcatatt ttcttaccgt ggcgcgatta cagttcagca
      721 ttcgtgaaac ttcggtcata ttacctcgtg tctggataag cagttccggt atcgtttgaa
      781 ttttgacgct catcaaatac tctccggttc ggtgattttt atcccgggct ttccaccagg
      841 gacataatga tcgcgcacaa gccagggcaa aacaaactca tggcgcatca gcgcagcgcc
      901 tcctgcacca gtttttcaaa ctttccgact ctggtttcca gctctgccac acagtccacc
      961 agctcatcta ctgctttctg tgcgcgatgc ttcgcctgca tcagttcccg aagcgcgggt
     1021 accatatctt tacggatagc atcttttgtt atgcccgttt tttcgagttg ttcagcatga
     1081 cgcagcattt cctgcgcgtg tttacgcaat tgttcagggg taaaagtcat tgtctggttg
     1141 ttcaaaagaa acgctccatc ttactgctgt cagttcgttt attactgtat ctgcgcggat
     1201 tgccgggctt catgggagtg gaaagcaccc gtgcactttc ctggtccaca ggcagaaaat
     1261 gtccgttata aaaacgccgg taaatcgttc ccagagaacc gttacgttgt ttcgtgatat
     1321 tgatttctgc gatgcccctg gcctgcgtat ccgggttgta cacttcatcc ctgtaaagca
     1381 tcagaatgat gtctgcatcc gcctctattt ctccggaatt tttcaggtct gagttcatgg
     1441 gacgtttatt gggtctggac tccacaccgc gggagagctg gctcagcgca atcaacggaa
     1501 aaccaccgga ttttgccagg cctttaagcc cctttgagat ttcacccacg gcaaggtcat
     1561 gacgccccgt ggttcgggtt tttatcagcc cgagataatc aaccaccacc agcgccgttt
     1621 ccggatattt aatcaggtgg tgtttcgttg ttgcgcatat ctcatcaatg gccaggttcg
     1681 cctggtccac catccagata ttgcgcccgg tcatccgccc caccccttgt gagaaacgcg
     1741 tccagtcttc atcttcaaag tgagccacag atttcaggcg tgatactggc atccctccag
     1801 ccgcagacac catacgttca ccaatctgga tgttcgccat ctccatggtg aacagaagca
     1861 caccatgccc ctgctcagtc accttgtcga tgatgtccag cgcaagttcg gttttcccca
     1921 tcgaaggacg ggcggcaatg aataccaggt ctccgggctc cataccgcct gtttttgcgt
     1981 ccagttcatc aataccggtc atcaacgtcc tggatttctc cagcccctga ttccggcatt
     2041 caacacgctc aaccacttcc ggaagcacat catcaatatg taccggctga atgacgccct
     2101 ttcctgtcga cagtgtgacc atcatgttct gcgcatcctt cagggcatct tcagctgctt
     2161 cacaggtatg cgcatcacgt aatttctgca gcgcctcatt cagtgttttt tctgcatcgc
     2221 gcagtgcggc attgcgccgc aacgctgcaa catagtgctc cagtgaagac ttcacccagg
     2281 ttttacgccc ggtatcagta atcaccgggg caagttccgg catctcatta cacaacagca
     2341 cggggtcaat cactcctgaa acacgggcct gtctgcagat gcctgtatag atatcctgat
     2401 acgctcgtac agaaaaaacg tccgctggca gtgtggccag aatatccatc acttcaggat
     2461 ctgctccgcg cagaaagaac gcgccgatga cagcgccttc aaggtcatcg ttacgccata
     2521 ctggagttgt catgcagcca cacctctgat acgagaacgg taactgggcc agttaaacga
     2581 caaccagttg cgtcccccgt ctgtgatcct gtcggcaatt cggggactga tgaacgccca
     2641 caactcttcc ggtgagaggt tactgataag aatggtgggc aagatacttt cgtacctggc
     2701 gttgataatt tcctgcaaaa tagccatttc agccgcgctg ccaaactgaa cgccaacttc
     2761 gtcgatgatc agcaaatcca ttgacgcata acgctcaata acttcatccg ctgttttttc
     2821 gctgtcattc cgccagcaat ttttcacagc acgggtaagg cgcatcacgt cggtgatctc
     2881 cacactggcc agatagttac ggatgatgtg ttttgccata gccacagcca gatgattttt
     2941 tccggtacca caactgccgg tcataacaag actggtaccg ttctccagca tatctggcca
     3001 gttctccgca tagcggcgac aggccgcaag atttctggct gcgccaggat taacctccag
     3061 ataattatca aactcgcagt cccgaaaacg cagggcaatt ccggcgttat cagtcagctc
     3121 ttccgccttg atggacgaca gctccatggt caaatcgttg gcctcagcga ttaagcagtc
     3181 agggcagcat gaaatttctt ctctgtcctc gccattacga tcgctccaca ccagtatatg
     3241 cgtgtgatat tcgccatgtt tttcgcaata tccgcgacct tcacgcatca ggcaggaacg
     3301 ataaggccat ggcttttcgc ccttctgagc aaatgcaatc tctgcccgta actcatccat
     3361 tcgcgcctgt agtctttttt gttgttcacg caggttaaac gtcatcatcg ctgtcacctc
     3421 agaatgtcag tttgtcactg gatttaccga atttgtcaga catggctccc aggccagcca
     3481 ggacatcgac ctgtcgctgt cgcccacctc tgggagcggc tggctgttgc cagaagtctt
     3541 cgaagtgacg atcgggtcca aagaacgtcg cagcctgctt cacgaactgt gtgccggtat
     3601 ttcctgtggc acgtacccag gcagcatagc gtttcacgcc atcaagcatg gcttcaggtt
     3661 ttattccctc cctgagccgg gctttccagg ctttgaaggc tgcggatttt gagttaccac
     3721 cagcacgttt tggatattcc tgccaggcct gttcaaattc cggtgaatat tcctgtcggg
     3781 aagaacgcgc tggtgcagac gcgtcagcgg atgcgccaat agtgttttta ctctctgtag
     3841 tattctctga agtaatctct gttgtattct ctgtaagatc gaaattggtt ttcccttctc
     3901 cgcggcgagg ggtttcccgt gtccgcggtg aaggctttcc ctcctccgcg aaattgggtt
     3961 ttacagtttc ccgaaaacgg gtttccccat ttcgggaaaa ctgattgttt tcattgataa
     4021 tttcattaag gcgctcacaa tctatacggt agaacatttt gtgctcaaga cgcttgttgg
     4081 tttcaaccaa aatgcctctg gacacaagat gcttacgcgc tacagcctgt tgttcaaatg
     4141 taagtccggt ttcgtgttgt atctcttcac gcgttttatg tacgccttcc gctgcatgtg
     4201 ctttatcctg ccagtaaaaa atctgaccaa agaaaataac agcgtgcgga cttcccatgt
     4261 atttaacgag cccagggtaa taagcaaccg gatgtccaaa atcgagcaga agatcagacg
     4321 gacgcatagc cacctcccag gcgtttaaac atttttccgg gctgaaacgc caccagcgga
     4381 taactcaggg tatgagtacg tccctgaacc tggcagacaa ccttctggct ttcggtactg
     4441 accaggcaaa cccgcagaac gtagccgttg ctggtggtga accactgccc cacacggggg
     4501 caatggttgt atcggtgata caggaaatta acgatgtggc ggatcatggg cgcacctcct
     4561 tgtcagaacc attcagcctg gaatcaacaa gtgcagcacc aaaaacagca tcacctacac
     4621 ggtcgtacag tttgccagcc agcggagatt caacggcctt aagcattggg taaagctggc
     4681 ttgtccagat ttgatggatt tcacgcaaat gcaggtatac gcctctggcg ttttgtgcga
     4741 cagatgacat gtcagccgca ccagctcctg ataagctcct ctccatctgg ttaaaggcat
     4801 tgatgtatgc ctctttgaac cgggcggcac gtttaccagt gaagcccata gcaaggaagg
     4861 cgaagccgcc gcgggtaatt tggtagcagg gaagtttgcg agtaccgccg ttgggctggc
     4921 gtaccaaaat tgatgtctcc gcaaaattgc gggcacaaaa ctctggagaa caatccaaaa
     4981 tgcggatctt tttcagaaca tcgtcatgac gtttagagaa gaagttggca acagccaggg
     5041 atgaagtaac agcctaacca tcaacgatgg caatttcagg ttgagagagg gttgggagag
     5101 tagtcatggt gacagcccct atgttgaatt caaagaactc accacatggg acgccaatca
     5161 cagaggtggt gagacgtaca gggttggcgt aaccggtcaa catagaaccc ggcgcatctt
     5221 gcgatgtccc tgcacgcccc accataattt gggcgtagca atgctcatga cacgaaaaaa
     5281 ccgcatgagc gcggttatgc tctatattga atttcaggac gccaatcccg gcccccgctt
     5341 tataaggtgc agagacagtg taacgtcccg aaattgcaga atcaatatat tgtgcagata
     5401 taacgatttt gttatgtttt gctgtatgaa ctacactatc gaatactaca gtgaagaggt
     5461 ccgactggag gtcgatcagc ttccattgag tatgcgtgcc agataccagc atcttgttga
     5521 acgcatgaag gtatacggca gcaatctcgg agaacctcac accagcgcct ttggtgacgg
     5581 gcttttcgaa ctcagaatta aaggcaacga tgggatcgcg cgtgtttttt actgcactct
     5641 gacagggaaa cgcatcatca tgctgcatag ttttgtaaag aaagcgcaga aaacaccgcc
     5701 agccgaacgc aagaaagctg aaaccagaat gaaggaggtt aagcatgact ggtaaacgca
     5761 ctctccccac catgacacac gacgaaatgg cagccaaatg gatggaagac ccggctttta
     5821 aagcagaata cgacgctatc gctgacgaat tcgcactgct tgatgaaatg ctggcagcac
     5881 gcaaagaagc tggcttaact caggctgaag ttgccgagcg aatgggaaca aaagcgaccg
     5941 cgatcaccag aatggaaagt aatctcgcat caggtatcag cggcccatca tttgccacac
     6001 taaaaaaatt cgcccgcgct actggaaaaa aactccagat ccgcttcgtt taaccacacc
     6061 gcgccgtcat tctggcggcg caccggataa atagtcattt cctcgcacga tgtcttagcc
     6121 accggatatc ccacaggtga gtcgtgtagt tgaaggtttt tacgtcagat tcttttggga
     6181 ttggcttgcg tttatttctg gagcgtttcg ttggaaggta tttgcagttt tcgcagatga
     6241 tgtcggtgaa acttcgtcga tgtcgcctca ttcatacctc ctgtcggtaa atctgacacc
     6301 ctgaccaata gcccaggctg ttgtgtactc gatcagactt gccatgcgct tcacgctcat
     6361 ctgcgcgctg ctttcgcgaa tgttgacgca ttctccttca aggccgggca aaacatcagc
     6421 ttcctgtttt gttgccactg catgaccgct gatcaacaaa accttccatt gttctggttt
     6481 taaccatttg ccgcaccatt gaacctgacg agcgatatcc gccagcatcg cgtgaaattt
     6541 tgcgttctgg tcaagatttc gcttgtagtc agtaatgcgg atggtgactg gcttgtcttt
     6601 atcgagagga gttgagagga tggcatttat tgcggcttgc tgttgttgct tagttcggag
     6661 gaagattgtt tgcttcatcg aaaattcttc
//
LOCUS       DADLCQ010000172         6685 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000172 DADLCQ010000000
VERSION     DADLCQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.174"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..74)
                     /locus_tag="JQG95_003699"
     CDS             complement(<1..74)
                     /locus_tag="JQG95_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HAY5890115.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNV"
     gene            complement(84..2273)
                     /gene="fadB"
                     /locus_tag="JQG95_003700"
     CDS             complement(84..2273)
                     /gene="fadB"
                     /locus_tag="JQG95_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY5890116.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            2463..3794
                     /gene="pepQ"
                     /locus_tag="JQG95_003701"
     CDS             2463..3794
                     /gene="pepQ"
                     /locus_tag="JQG95_003701"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY5890117.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            3794..4408
                     /locus_tag="JQG95_003702"
     CDS             3794..4408
                     /locus_tag="JQG95_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY5890118.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            4447..5898
                     /gene="trkH"
                     /locus_tag="JQG95_003703"
     CDS             4447..5898
                     /gene="trkH"
                     /locus_tag="JQG95_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY5890119.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            5910..6455
                     /gene="hemG"
                     /locus_tag="JQG95_003704"
     CDS             5910..6455
                     /gene="hemG"
                     /locus_tag="JQG95_003704"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY5890120.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1496 a         1747 c         1818 g         1624 t
ORIGIN      
        1 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
       61 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
      121 tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
      181 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
      241 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
      301 agcgccatat ccgcttccgc cggagtggcg ataatgccct cttccagaca gcgcaccact
      361 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
      421 ggttggctca cttctgccag caggtcttca acggcggcgt cttcttcttt cttcggctta
      481 cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
      541 gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct
      601 gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacaacgtc cagcagatat
      661 gccgggccca tcggccagcc aaactgtttt tccatcactt tgtcgatctt gcggaaatcc
      721 gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac gcggttaaca
      781 aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct cgcccaggcg
      841 acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc taccaacggc
      901 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttccgggcg ttccagcgcg
      961 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt atcctggcgt
     1021 actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc aacaaccgct
     1081 tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga gcgttgggtg gatcgtggag
     1141 atcacgccag ccagtttcag accatcgatt ttgccgcgct caagctgctt gttcagcagt
     1201 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
     1261 ggcacgcctt tccacgcgga ctggtaagcg ataccgccac ccatgatgcc tgcgcccagc
     1321 acagcagcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
     1381 tattgatcgt taaggaaaat gccgaccagt gcgcgagctt cgttggtatg cgccagcggg
     1441 acaaaacttt tgttttccag atttaaggct tcttcacgac caaaacgggc cgcagcttca
     1501 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgccgtttg tgcgaccatt
     1561 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag cggttcctgc
     1621 ttcggctgac gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc
     1681 atcgcccctt caatcagttt ttctgctttg actacgccat ccaccagacc gattttcagc
     1741 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagtgcact gtcagcgccc
     1801 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
     1861 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
     1921 cagccaccac ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
     1981 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
     2041 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
     2101 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
     2161 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
     2221 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
     2281 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc ggagtgtgtg ttttgtgtta
     2341 aaaatatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
     2401 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
     2461 aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa gaacggactc
     2521 gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc gagctgttca
     2581 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
     2641 tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac aagccgaaac
     2701 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct
     2761 ggactgaaga tgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
     2821 ctgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg ctgcaactgg
     2881 gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat tattaccgct
     2941 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaatg
     3001 gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg
     3061 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
     3121 ttaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
     3181 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
     3241 gtacctggtc ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg
     3301 aacaactggc gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccacatcc
     3361 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
     3421 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
     3481 cgctgggcct gcaggtgcat gacgtcgccg gttttatgca ggatgatagc ggtacacacc
     3541 tcgcggcacc ggcaaaatat ccgtacctgc gctacacccg tattctccag ccgggcatgg
     3601 tgttaaccat cgaaccgggt atctacttca ttgaatcgct tctggcaccg tggcgtgaag
     3661 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
     3721 ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt ggaaaacatg acccgggatc
     3781 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
     3841 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
     3901 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
     3961 ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg gggagccggc
     4021 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg gggaaattac
     4081 cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
     4141 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
     4201 aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg
     4261 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
     4321 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
     4381 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
     4441 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
     4501 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
     4561 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
     4621 aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct gttttggacc
     4681 gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
     4741 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
     4801 gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca atggtttggc
     4861 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
     4921 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
     4981 gcggaaacgg cgaaaactct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
     5041 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
     5101 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag tccgactatt
     5161 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
     5221 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
     5281 ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca taatgtctac
     5341 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat ggcgacaacc
     5401 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
     5461 tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa agtgatccgc
     5521 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
     5581 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
     5641 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
     5701 ggcgtggatg atttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
     5761 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
     5821 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tactggtgct ctttaccccg
     5881 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
     5941 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
     6001 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaac tatgaccgtg
     6061 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
     6121 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
     6181 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
     6241 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
     6301 atcgctggta cgaccgtttt atgctcaagc tgattatgaa gatgtcaggc ggtgaaacgg
     6361 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
     6421 tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt
     6481 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
     6541 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaattccg
     6601 acagagaaag caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgaacaccct
     6661 gcgccgctga gaaaaagcga agcgg
//
LOCUS       DADLCQ010000173         6619 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000173 DADLCQ010000000
VERSION     DADLCQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.175"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(53..532)
                     /gene="xdhC"
                     /locus_tag="JQG95_003705"
     CDS             complement(53..532)
                     /gene="xdhC"
                     /locus_tag="JQG95_003705"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY5890121.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="JQG95_003706"
     CDS             complement(529..1407)
                     /gene="xdhB"
                     /locus_tag="JQG95_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY5890122.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="JQG95_003707"
     CDS             complement(1418..3715)
                     /gene="xdhA"
                     /locus_tag="JQG95_003707"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY5890123.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIDWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4130..4885
                     /locus_tag="JQG95_003708"
     CDS             4130..4885
                     /locus_tag="JQG95_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY5890124.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4964..5037
                     /locus_tag="JQG95_003709"
     tRNA            4964..5037
                     /locus_tag="JQG95_003709"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4996..4998,aa:Gly,seq:ccc)
     gene            5152..>6619
                     /locus_tag="JQG95_003710"
     CDS             5152..>6619
                     /locus_tag="JQG95_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5890125.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSVSLRT
                     KDRRTAMAHSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDR
                     FNDIDRQDDQADSVSLSVSAPEVKPEVTPSITIASLFEQYEAENSQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLKVRDVLQQLPKNRKQRFKDAPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVATQLK"
BASE COUNT         1646 a         1646 c         1722 g         1605 t
ORIGIN      
        1 gttaaacaca taacctcagt gaatccggcc tgaattcagg ccggatatcc ttttacttcg
       61 ttttctcgca atccagaact gtatttacaa tcatctgata ccccgtgcag cgacaaagat
      121 ttcccgccag tccgcgacga atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca
      181 gcattgccgt ggtagccata atcaggccag gcgtacaaaa cccgcactgc actgtgccgg
      241 atttcgcata agcctgctga acatgagaaa gttttccgcc tttcgcttca ccttccagcg
      301 tgcggatctc ttttccttca gcccaggcgg caaggtataa gcaactgtct attgctgtgc
      361 cgtcgaccaa caccgtacag gcaccacatt cacccacgca gcacccttgt ttgacactta
      421 gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc tggtgcggcg tgaagctgaa
      481 aaggcatccc gttaatggtg cattcggtgg taattgtttc gctgtgattc attgcaattt
      541 tccccccgcc gcggcgacgg cttcgctaat cacttttttg gtcatcgtct ggatgagatg
      601 cagacgaaac tctttactgg cccgccatga agaacgcggg gcgacatctt gcaggacaga
      661 ttcgctgata gcttccagcg tttgcaggtt taatggcgca ttttgtgcag tctgttcggc
      721 atgttggcag cgaatcggcg ttggcgcggc aacaccaaat gccaggcgta attcgctgaa
      781 attgccgtta tccagtcggc aatgtgcggc gcagccaatc gttgaaatat ccattgcgtc
      841 gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct ttcggctgtg gcggaaaatg
      901 aaaggcgacg aggatttcgt catgctcaag agacactttg cccggcccgg tgtgaaagcc
      961 attaatcggg acgaaacgaa caccgcgcgg ggagtggatc tccagtttcg cgtcataaat
     1021 tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg caaatatttc caccgtaggt
     1081 agcgacgtta cggatctgcg gtccagcaat gaacgtggcc gcagcacata acgccgggag
     1141 atgacgttga gttataggat cttctattag ctgggtaaat gtcgttgcag agccgatacg
     1201 tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc gccagattat gaatatcaac
     1261 aatatggcga taacggtcat tgtgatggtg gagctgaatc agtacgtcag tgccaccggc
     1321 gagcagtttg gcctgcgggt tgtcagccag caggttgatg gcatcggcaa gggttgctgc
     1381 gcgatggtaa gaagcaaaat caaacatgat gttatcctca aatcaatcct gccagatgga
     1441 actcttcata taaccgtttt ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca
     1501 tcttcaccgc gttacgaata gcagcggcaa caggaattat gggtggctca cccagtgact
     1561 tatgtccgta tgcggattgc ggctcattga tttcgacgaa cgcgctttcc agttgtggca
     1621 gatccggcat ggtcggcatt ttgtaatcca gcagattggg gttacggacc acgccgcttt
     1681 tagcatcgat gatcatctct tcaaatagcg cccagtcaat gcccattccc attccgccgt
     1741 gtacctgacc ttctgccagc agtggattaa gaatatgccc tgaatcatga acgttgagga
     1801 tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt cagatcaaca aaggtacagc
     1861 caaacgccgg tgggttagtg gtggttttga tggagctttc agcagagagc tgcccgccgc
     1921 gttcagggtg gtagaaagcg tccatcgcca aatcttttaa cgacattaac ggctcttccg
     1981 gtcgttcaac cagcacgata tggcctttta tcagggtcaa attcatcgct gactgatgta
     2041 gcatgactgc ggcgtgagcg atgattttct cttttaataa tagtgccgca ctgcgcagcg
     2101 caggcgcggc aacatagctc tggcgtgagg caaatgcgcc gggatcgaac ggcgtaacgt
     2161 cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg aacccccacg gtttctgcca
     2221 ccatttgcga gaagacggtg tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt
     2281 tgatggttcc atcctgattc atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc
     2341 aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc gcggcgcaaa ttgccttgct
     2401 ggttctggca ttctgcacgg cgtttttccc attcaaagat tttccggcct ttttcaagac
     2461 actccggcaa ccctgcgctg caaatacgtt tgcccgtgag cggattagca tctccttcgc
     2521 gggcggcgtt gcgtaaacga atttcaacag gatcaatacc taacgctgtc gcggcgtcat
     2581 caagcataga ctcaacggca aatacgactt gtggcgcgcc ataaccacgc atcgcaccag
     2641 ccgaggggag gttggtatag caggtctttg aactgtaagc gtaggcacaa cgaggataaa
     2701 ggtaagcgac tttattcccc cccgcagaag cgatggagtg cccgtgagat gcataagcgc
     2761 cggtgttaga cagaacatcc agactataac ctttcaaggt tccgtcgcgg ttcacgccca
     2821 tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc gaggaaacac tcttcacggc
     2881 taagggaaac tttcaccgga atgccgccaa gcttgctggt caggaatgcc gccattggct
     2941 cttccagtac atcctgttta ttaccaaaac cgccaccgac aaacggtttg atgactcgta
     3001 cgcatgacca gggaatatcc agcgcctgac caaccacgcg gcgaacaatg tgcgggatct
     3061 gggtgctgga aacgatggta attcgcgagt catcctccat ccacgccagc gatgttacgc
     3121 tttccatgtg acaatgttga ataacgggag tctgatagtg cccctgtacc tggtagtcgg
     3181 cggcatcgat tgtttgttgg acattgcccg tcgacatcgt gctttgtttc agtaaattgc
     3241 cgccgttatg gattggtgca gcgtcttctg tcagcgccgc ttctggcgtg gtgataacgg
     3301 gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc
     3361 gggccacgac gatggcaacg gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc
     3421 gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc atgcccagct gtagcgaatg
     3481 gaatatcagg cacatcctcc caagtaaaaa tcgccagtac gcccggtaaa cttctggctt
     3541 gttcatcatt aatacttacg gcataaccat gtgcgatagg gctacgtaca tatttcgcgt
     3601 aacacatgcc cgccataaca taatcgtcag tatatcgtgc ccgcccggtg accttagcaa
     3661 tggcatcgac gcgcatgcat gattcacccg tagcggttgc ttcccgcgct tccatagaaa
     3721 tcccctcgat ttgaacattt ggttcttatg gcaaagattt agtctaagca ggcaagattt
     3781 acgccagaaa cgacgataag cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca
     3841 cagatatgat acgaaacagg actctgaata gggacagtga tacgtatcaa tatgagtggc
     3901 gataaaatca atgtgataaa tcaggattat tacgcatcag gatgggaaat agggtaaaaa
     3961 gtcaggaact tcgatacctc tacagcgaat attttgctga tgatacttcc ctttgccaaa
     4021 tgaaattaac ctctttctct attaccacgt ttttccagaa gcaagagatt gggtatcaag
     4081 aggctggctg ctatgatatg gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg
     4141 acgcctgaat aaaaaatctc tgggtatcgt gatgttttta tcggttggac tgctattggc
     4201 gggctgttcg ggtagcaaat catccgatac aggaacgtat tccggctccg tttacaccgt
     4261 gaaacggggg gatacgctat atcgtatttc gcgcaccacg ggaaccagcg taaaagagct
     4321 ggcgcgactg aacggcattt ccccccctta caccattgaa gttggtcaga aactaaaact
     4381 gggtggggcg aaaagtagca gtagtacacg taaatcaacc gccaaatcaa cgactaaaac
     4441 cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc tggccgccag tagggcaacg
     4501 ttgttggtta tggccaacga cagggaaagt tatcatgccg tattcgacag cagatggcgg
     4561 caataaaggg attgatatct cagctccacg gggtacacct atttacgccg cgggtgcagg
     4621 aaaggtggtg tatgtgggca accagctgcg tggctacggt aatctcatca tgattaaaca
     4681 cagtgaagat tacattacgg cttacgccca taatgacacg atgctggtaa ataatgggca
     4741 aagcgtgaag gctgggcaaa aaatcgccac tatggggagc acggatgcgg catctgttcg
     4801 cctgcatttc cagattcgtt accgtgcaac ggcaattgat ccgctacgtt acttgccgcc
     4861 gcagggcagc aagccaaaat gctgatggcg aattaatcag cagtcagcag cgcggcactt
     4921 gctaaggaga gcgtaaggtt tataatgcct tacgcatctc gaagcgggcg tagttcaatg
     4981 gtagaacgag agcttcccaa gctctatacg agggttcgat tcccttcgtc cgctccatga
     5041 cctttcttaa agtactccaa cctactgaat cctaagggat atggctttaa gcctccttgc
     5101 agagttactt actgtaaccc actgtgtaac cctctcaggg catacagaca tatgattcag
     5161 ctatccagta gtcacaaact tccagcagtc tactatctct accagcgtaa cggcgtctac
     5221 tacttccgtc ttcgggtcag acaatcaaac aatgatagaa tgacctcagt atcattacgg
     5281 accaaggata gacgcaccgc tatggctcac tcaaggcaca tcaaggcagc actcaaggca
     5341 atacacgcag acagacccaa cgctacctat gaggagatga gggagcacct taaggacatc
     5401 gctgagtgtg aactgtctat gggccgttca gatctctttg agcctgacat gagggatatc
     5461 taccgggacc aatacggaga acttggtgag agccttaccg atgctctggc tagtgagccg
     5521 ctaagtattg accagcatcg ttacatcaat gaggccctga aggtgctaaa agcctgtatg
     5581 aggcgtatcg aagcgggaga tagtcagcct ctgatagatt acgtcgatcg ttttaatgat
     5641 attgatcggc aagatgacca agcagactct gtgtccctat ctgttagtgc tccagaagta
     5701 aaaccagaag ttacgcctag cattaccata gcgagcctct ttgagcaata cgaagcggag
     5761 aactctcaga actggaagcc tgcaacctta agggaaaaca aagcgtcaca cgctgcgtta
     5821 atcgaaatat tcgaccatct gggtctcaat gcagacgcta acagggctga catgcttaag
     5881 gtccgtgatg tgctccagca gttacctaag aaccgtaagc agcgctttaa ggatgcccca
     5941 cttgctgacc tgttgagccg tgaggataag acagattgtc tggatgtcgt taccatcaac
     6001 aacaagtatc ttattaagat ggctgctgtc tttaggtggg ctgtgcgcaa tgacttgatt
     6061 aaaaagaaca tgactgaggg tctggagctt aaagtccctc agcgtaaagc ctcaggggcc
     6121 cgtaacgcct tctctacgga gcaggtgggg caactactgg tcgcagctaa ggcatactct
     6181 cagaagacct ctggtaagcc ctaccactat tacgttacgg ctttggctgc tatcactggg
     6241 gcgagactca atgaaatagc ccaactacag gtcaaagacg tcaggaccac tgaggcggga
     6301 accgtttata ttcatatcaa tgaagacgat agtagtctac ccggtaagag catcaagaac
     6361 gctcatagtg accgctgtgt gcctctggtc gatggggcct atggttttat attggctgac
     6421 tttatggcgc ttgtagagac ccgtagaggc gctgacggtg atgacgctat ggtgttcgat
     6481 ggtctgcgtc tcatgaagaa cggttacggt gagcaggtga gtaaatggtt taatcggacg
     6541 ttgttgccta aagtcctcgt agaccgtagc ggcttagcgt ttcactcgtt tagacataca
     6601 gttgcgaccc agttaaaac
//
LOCUS       DADLCQ010000174         6617 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000174 DADLCQ010000000
VERSION     DADLCQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.176"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            62..2494
                     /locus_tag="JQG95_003711"
     CDS             62..2494
                     /locus_tag="JQG95_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY5890126.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2525..4030
                     /locus_tag="JQG95_003712"
     CDS             2525..4030
                     /locus_tag="JQG95_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY5890127.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4030..5691
                     /locus_tag="JQG95_003713"
     CDS             4030..5691
                     /locus_tag="JQG95_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY5890128.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5912..6253
                     /gene="symE"
                     /locus_tag="JQG95_003714"
     CDS             5912..6253
                     /gene="symE"
                     /locus_tag="JQG95_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY5890129.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6300..>6617)
                     /locus_tag="JQG95_003715"
     CDS             complement(6300..>6617)
                     /locus_tag="JQG95_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890130.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         1951 a         1580 c         1525 g         1561 t
ORIGIN      
        1 atagccaggc tattgatttt atgtacaatc agaatcgtcc tgataaaagc gagaaccccc
       61 catggcggaa ctgaacctaa gtaacctgac ggaagcagac atcattacca aatgcgttat
      121 gccagccatt ctcaatgcag gctgggacaa cacaacgcaa atcagacagg aggtcaaact
      181 ccgggacggt aaagtcatcg tacgtggtaa agttgcggca cgcagaacgg taaaatctgc
      241 tgacatcgtg ctgtaccaca agccaggcat tcccttagcc gtgattgaag caaaagccaa
      301 caaacatgaa attggcaaag ggatgcaaca gggcattgaa tatgcgcgcc tgctggacgt
      361 tccttttgtt tttgccacca acggtgatgg ctttatcttc cgcgatgcca ccgcagccga
      421 aggtgaatgc cttgaaaagc aaataacgct ggatgacttc ccctcccccg ctgaactctg
      481 gcaaaaattc tgcctctgga aaggatacac acaagctcag cttccggtga ttactcagga
      541 ctattacgac gatggcagcg gcaaatcgcc tcgttattat caacttcagg caatcaacaa
      601 aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg ctggtcatgg cgaccggaac
      661 agggaaaacc tataccgcat tccagatcat ctggcgcctg tggaaatcaa aaaataaaaa
      721 acgcattttg ttccttgccg atcgcaacat tctggtcgac caaaccaaaa ataatgattt
      781 ccagccattt ggtacggcaa tgaccaaagt cagcggacgc accattgatc ccgcttatga
      841 aattcacctc gcgctctatc aggctataac tggcccggag gaagaccaaa aagcgtttaa
      901 acaagtcgca ccagatttct tcgatctgat cgtgatcgac gaatgccatc gcggcagcgc
      961 atccgaagac agcgcctggc gagagatcct tgattatttc agttccgcca cccaaattgg
     1021 cttaaccgcc acgccaaaag agacgcatga agtctccagc acggattact tcggcgatcc
     1081 ggtttacgtc tactcactaa aagaagggat cgaagacggc ttcctcgctc cttataaagt
     1141 tgtccgtgtt gatattgatg tcgacctgca aggctggcgc ccaaccaaag ggcaaactga
     1201 cttaaacggc gaagtgattg acgatcgtat ctataaccag aaagatttcg atcgcacgat
     1261 ggtgatcgac gaacgcacag aactggttgc cagaaccatt accgactacc tcaagcgcac
     1321 caatccgatg gataaaacca tcgtcttctg taacgacatc gatcatgcag aacgtatgcg
     1381 ccgcgccctg gttaatctca acccgcagca ggtgaaaaag aacgacaaat acgtcatgaa
     1441 aatcaccggc gatgatgaaa ttggcaaagc tcagttggat aacttcatca acccgaaaaa
     1501 accgtacccg gttatcgcga ccacttcaga gctgatgacc actggtgtgg atgctaaaac
     1561 ctgcaaactg gtagtactag accagaacat ccagtcgatg accaaattca agcaaattat
     1621 cggtcgtggt acacgcatcg acgaacgtta cggcaaactc tggtttacca tcctcgactt
     1681 taaaaaagcc accgaactgt ttgccgatga gcgtttcgat ggcattcccg aaaaagtcat
     1741 ggataccaca ccagaggata tcgccgatcc agaatctgat tttgaagaga aactcgaaga
     1801 aatcagcgaa catgacgacg aacaggtaac aggcgttgat gaaccgcctg cgccaccata
     1861 ccaggttacc gataccgatg atgtcggccc atttccggaa gaagacgaga agaaaatccg
     1921 caagtttcac gtcaacggtg tagcggtggg cgttattgca cagcgcgttc agtattacga
     1981 cgccgacggt aaactggtta ccgaatcctt taaagattac acccgcaaaa cactgctcaa
     2041 agaatatgcc tcgctggatg actttacccg caagtggcag gacgccgatc gcaaagaagc
     2101 gatcattcac gagctggagc aacaggggat catctgggaa gtactggcag aagaagtcgg
     2161 taaagatctc gacccgttcg acatgctttg ccacgtagtg tatggtcagc cgccgttaac
     2221 ccgcaaagag cgcgccgaga acgtgcacaa gcggaactac ttcacaaaat actctgaagc
     2281 agcgcaagcc gtgctcgata atctgctgga taaatacgcc gatgcgggcg tacaggagat
     2341 cgaaagtatt caggtgctga aacttaaacc attcgacagc atgggcacct taccggagat
     2401 tattaaaacc ggatttggcg accgtaacgg gtataatcag gcgctcagcg agctggaaaa
     2461 cgaaatctac caattaccgc cccgctctgc ttaacaactc tcttttgcgc cttctgcaac
     2521 catcatgttg cagaaggcct tgtttttcat ggaatacaac atgtctatca gctcagtaat
     2581 caaatcatta caagatatta tgcgcaaaga tgccggtgtg gacggcgatg cgcagcgtct
     2641 cggtcagctc tcctggctgc tgtttttgaa aatcttcgat gcccaggaag aggcgctgga
     2701 actggagcag gataactatc aatatccgat cccacagcgt tatttgtggc gcagttgggc
     2761 cgccaacgcg cagggcatta ccggtgattc cctgctggaa ttcgttaatg atgatctgtt
     2821 tccggcgttg aaaaacctca ctgcgcctat cgataaaaac ccacgcggct acgtggtgaa
     2881 gcaggcgttc agcgatgcct ataactatat gaaaaacggt acgctactgc gccaggtgat
     2941 caacaagctg aacgaaattg actttaccag cgccagcgaa cgccatctgt ttggtgatat
     3001 ttacgaacag atccttaaag atctgcaatc tgcgggcaat gcgggcgaat tctatactcc
     3061 acgcgccgtc actcgcttta tggtggatcg cgttgatccg aaactcggcg aatccattat
     3121 ggacccggcc tgcggtacgg gcggttttct tgcctgcgcg ttcgatcatg taaagaacaa
     3181 atacgtgaag agcgtcgccg atcatcagac actgcaacaa cagatccacg gtgttgagaa
     3241 aaaacagctt ccgcacctgc tggcgaccac caatatgctg ctgcacggca ttgaagtacc
     3301 ggtacaaatt cgtcacgaca acaccctgaa taaaccgctt tcctcctggg atgagcaact
     3361 ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa gaagacggta ttgagaagaa
     3421 ctttccggca gagatgcaaa cccgcgaaac ggcggatctg tttctgcaac tgattgtgga
     3481 agtactggcg aaaaacggtc gtgcggcggt ggtattgcca gatggcacac tatttggcga
     3541 aggtgttaaa accaaaatca aaaagctact taccgaagag tgcaacctgc ataccatcgt
     3601 gcgtttaccg aatggtgtgt ttaatcccta taccggcatt aaaaccaacc tgttgttctt
     3661 taccaaaggt cagccaacca aagagatttg gttctatgaa catccgtatc cggcgggcgt
     3721 gaaaaactac agcaaaacca agccgatgaa gtttgaagag tttcaggcgg agatcgactg
     3781 gtggggtaac gaggccgatg gttttgccag ccgcgtcgag aatgagcaag cgtggaaagt
     3841 cagcattgat gacgtgattg cgcgcaactt caatctggat attaaaaacc cacatcaggc
     3901 ggaaaccgtc agccatgatc cggacgaact gttagcgcag tatgcaaaac agcaggcgga
     3961 gatccagacg ctgcgtaatc aactgcgcga tattcttggc gctgcgctgt ctggcaagga
     4021 ggttaactaa tgagtgtgga aaagctgatc gttgatcata tggaaacctg gacctcggcg
     4081 ttgcaaaccc gttccaccgc cgggcgcggc agctccggta agattgattt gtatggtatt
     4141 aagaaattgc gtgagctgat tctggaactg gcggtgcgcg gtaaactggt gccgcaggat
     4201 ccgaatgatg aaccggcgtc ggacctgctg aagcgtattg cggcggaaaa agcagagctg
     4261 gtgaagcaag gcaaaattaa aaagcaaaaa ccactgccag aaattagtga ggaagagaag
     4321 ccgtttgaat tgccggaggg atgggagtgg gtgcacctcc ctgatattta ttgctcaatt
     4381 tctgagtcat ctagaaaaat aaaatcctcc gaaatactac ctgagggaaa atatcctgtt
     4441 atcgagcaaa gccaagaatt tatttctggt tattgcaaca atgaatgtct gttaataaag
     4501 ttaaataatc cagttatagt ttttggtgat cacacaagga atattaaatt tattgatttt
     4561 gattttgttg tcggcgctga cggtgtgaag attttatcac ctatattaat ttgtgagcgt
     4621 ttcttttttt ggcagttacg atcatttaaa ttagatgtaa gaggatacgc gagacatttt
     4681 aaagtattaa atagctgctt atttgcatta ccccccattg cagaacaaga gcgaatagtt
     4741 gaaaaagtta gctctttgat gtccctctgc gaccaactag aacagcaatc cctgaccagt
     4801 ctggacgcac atcagcaact ggttgaaacc ctgttgggaa cgcttacaga cagccaaaac
     4861 accgcggaac tggctgaaaa ctgggcgcgt attagcgagc atttcgacac cctatttacc
     4921 accgaagcca gcgtggatgc gttaaaacag accattctgc aactggccgt aatgggcaaa
     4981 cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac tgctcaaacg tattgcgcag
     5041 gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac aaaaaccact gccgccaatt
     5101 agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg aatggtgtcg tattggtaat
     5161 atagttaata ttaaatctga attagtatca ccaaaagatt atttaaattt atatcaagta
     5221 gcacctgata ttattgaaaa gggcactggg agagtgatat caaagagaac agtgaaagaa
     5281 tcaggagtca aagggccaaa tagtcgcttt tataaaggac aaattgttta ttctaaaatc
     5341 cgcccgtcat tatccaaagt ttttttagca gagtataatg gtctttgcag tgctgatatg
     5401 tatccattag actgttacat taatccaaat tacttattga aatatatcct ttccattcca
     5461 tttcttatgc aagtcaaaaa agcagagaac agaataaaaa tgccaaaact taattctgac
     5521 agtttttaca atattattgt tgcgattccc ccctataatg aacaacaagc aatatttgat
     5581 aagataaatt ctattgaagc agtatgcaat ggtcttattt cttatattgg gatttatcat
     5641 aaaacccaac tgcatcttgc agacgcactc actgacgcag cgataaacta attccctcgc
     5701 cctcacaaaa ccaataaagc ggctaaatag ccgctttatt cacatcacca aaatttatat
     5761 ttcacgctaa ttttcatctt attttcatta atttttagct ggactttccc tgtatttact
     5821 gatgatgtat ccaggtattt agcacggtgc ggatgtgcgc caacacactc gcaccgctaa
     5881 tcacaatcac tatttataga gaatagtaat catgactgac acgcattcta ttgcacaacc
     5941 gttagaagca gaagtctccc cggcaaataa tcgtcaatta accgtgagtt atgcgagtcg
     6001 ttacccggat tacagccgta ttcccgccat caccctgaaa ggtcagtggc tggaagccgc
     6061 cggttttgcc accggcacgg cggtagatgt caaagtgatg gaaggctgta ttgtcctcac
     6121 cgcccaaccc gccgccgccg aagagagcga actgatgcag tcactacgca aggtgtgtaa
     6181 gctgtcggcg cgtaaacaaa ggcaggtaca ggaatttatt ggggtaatta caggtaaaca
     6241 gaaagtcgcc tgatacattg acccggtagc gccagtgcta ccgggtatta ttcgccgcgt
     6301 tataccaacg cgctctccat ctgtttttca aatgcctgac gaattaatcc cataatgtaa
     6361 ggaagttgtg cgaggtcgct gaaaccaatt tctacatcgc cattacccca gcgcccaaca
     6421 tttgtcacat ctttggcaat acctttcgga tcgaccagtt catgaaactg catattaagc
     6481 gacaatcgca gtcggctttt ttgcggcaca acatcaacaa aattcgtttc cgctttaaac
     6541 gcaatatata gcttcaatac ttcctgcgtg atccctgcgt ccaggcgcat aacttcatcg
     6601 cgaagatgat cgaataa
//
LOCUS       DADLCQ010000175         6615 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000175 DADLCQ010000000
VERSION     DADLCQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.177"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(97..2100)
                     /locus_tag="JQG95_003716"
     CDS             complement(97..2100)
                     /locus_tag="JQG95_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY5890131.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLSSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2097..3233)
                     /locus_tag="JQG95_003717"
     CDS             complement(2097..3233)
                     /locus_tag="JQG95_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY5890132.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALINVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3226..5505)
                     /locus_tag="JQG95_003718"
     CDS             complement(3226..5505)
                     /locus_tag="JQG95_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890133.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5516..6604)
                     /locus_tag="JQG95_003719"
     CDS             complement(5516..6604)
                     /locus_tag="JQG95_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY5890134.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1511 a         1823 c         1777 g         1504 t
ORIGIN      
        1 actggtgact ctcaccaggg gctatatagg atatgcctaa taccgtggcg tgagcagtcc
       61 ggaactggag tagaactctt agtaaaaagc actatttcat ccttgttgct gaagcatggg
      121 gaataattgt tcgcaaagca aaacaccgtt attcattgct tctacccgtg cctcgctttc
      181 tgtattacga aattgtgcca acacatgtgc cagccgataa aaacccaccg cggtgaggtc
      241 attcgccagc aactctgcct gattaatagc gctctgttcc tgatagcgcc agccgttatg
      301 gagcagttga ataagtaacg cctggcagcg catcagcaac tgatgagcag tagacggcac
      361 aggcagaacg ctggcagaag atagtgatac caccacaggc gcagtttctg cgtccagcgc
      421 ccaggcacga gtctttgtca tcattacctg tggttccagt gtcaattgcc catcaacaaa
      481 actgacaaag ccagaaacca gacacacggg gtcgtctgtt tgttgcaaaa gcgccgccat
      541 gcgttcaatg gcataaggcg cgctggctga ggctggcagt gataacgtca gcagattatc
      601 ttcaccttcg ccgctgatta cctgcgcatc cagcgtctgg cggctgctat cccaaccgag
      661 cgaaatacac tcagcgaccg gcagaataaa taagttatcg acctgattaa gaggccgtat
      721 gcaggcgggg ggacgctggc gtaaatattc ccgcaaagcc acaatgcccg gctggcgtaa
      781 cggcgcgctc aacatttgcc aggcatcagg cgacagcggt acaacgctgc ttaagcggtt
      841 gcgggtagct aacagcagct cgccatcggc actgcgtttt gctgcttgtg aaacaatttg
      901 cccacccgcc agtgcgccag cctgaaaact aaacagccga cgcgtagctg ccggtgagtc
      961 ttcctgttca cttcgcggcc aactgcgcga aaggtgcaaa atactgccgg tgtcgggatc
     1021 ggtaaaccag atgcgtaaac cataatgctc aatatcctgc cagcaacgca tacctaaaga
     1081 caccagccgc agatgatcaa gctttgcttc tccggcaatg ccagagccaa cgaccgtgcg
     1141 ccacggcaca ggaggaactt caccaacact gtcgctccgg gccatctctt gtgcgcaatt
     1201 taatcgactg tttaatgccg caagctgacg taagcattct ccggcatgat agtggctggc
     1261 gcgggcgtgg aaggcatcaa cgctggcgcg cagttgccgt agcgattcac tcacccatcg
     1321 ccagctgcag cgttccgctg cttgctgcgc gcggctgaaa gcggcctcgt agtgaataag
     1381 cggctggctg atgccgctca gccataatgc ctggcttagt tgttgaacat attgacgaca
     1441 cgttttgcct tcttcgctgg caaacggatc gtcagatgat gtgacgtgtt cgctgcgcat
     1501 ctgccagatt aaatgagtca attccgcttg ctgcgctttt gcctggacaa aggcctgtac
     1561 tgccagtacg acatgttcgc aaagtgtgcc ttcaatacaa tcacaacggg cgaaacgaat
     1621 actgctgcgg gaataaaaac gcacatcgct catcggtaag cgggcagagg gaatttcacc
     1681 cggcgcacag aacaactcaa tggtgatgct tttagcgacc agcgcctgtg cgcgtttgcg
     1741 ggtggcatcg ggaagggtag ccagttcttc cagccagatt gccggatccc attcctcttc
     1801 ttttcccgta ggctgggcgg tagcacaaag tcgttgataa cttaacacca gcatcacgcg
     1861 atgacggcac atgccgctgg ccccataggt gcactgagcc tctttcagtg cctggccgtt
     1921 cgccagctgg gtacggacac cgtcactgaa ggtggcgatt aaagcgctgt tctcatggct
     1981 gatttccggg acgttgccat tttccagttc cttaaggctg cgcttaacaa aaccggcatt
     2041 gcttaacgcc gtcagtgcct gcggtgtcag ttctaataat tccggacgta gtgaattcat
     2101 gactgaagat tctccgcaag ccatgatgcc agctcgcccg gtgtcatggc ggctatttgt
     2161 gcgccgacat taatcagcgc ctgggccgta tcgcggtcat agcaaggtgt tgctgtgcta
     2221 tcgagcgctg ccagtcccag cactttgatg ccgctctgga cacacttttt cacttggtgc
     2281 gtcagcaatg atgatgaacc cccttcataa aaatcgctca cgaggataat gacgcttttt
     2341 gcaggttgtt caataagttg ccgaccatac tccatggcac tggcgatatt ggttccgccg
     2401 cctaattgca ctttcattaa taactctacc ggatcggcaa cgtctgccgt gagatcaacg
     2461 acgcttgtgt caaacgccac cagatgggta cgaatgccgg gtaactgcca taaacaggcc
     2521 gccatcaccg cagagtggat caccgaatcg accatcgatc cgctttgatc aaccagtaag
     2581 accagttgcc attgctcgct ttggcgttta atgcggctgt taaagcgggg gaattcgata
     2641 tacaacttgc cgtgttgcgg gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa
     2701 tcaaagtttc gcgccagtgg aataaatgag cggcgacgac gatcgcggac accagaaaaa
     2761 gcctgacgaa cttcctttgc cagtcgcgcc ataatttctt ccacaacctg gcgcacaatc
     2821 ctgcgggcgg cagccagtac ttcggggttc atcagatgtt tggtgtgcaa aacggcgcgt
     2881 agcaggcttt cggaaggctg catacgttcc agcacgtcga gatttgtcac cacatcttca
     2941 atgccgtagc gcagtacggc atcgctttcc agccgctcaa tcacctgttg cggaaacagc
     3001 gtgtgaatac tgttgatcca ctcaggagtg gtgagatttg agccacctaa tccactagag
     3061 cgttcaccac gctggagccg ttcaggatcg cgcccataca gccactccag cgcgtgatct
     3121 atctgccggg cgttgtcatc cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt
     3181 aatcgccagc gttgtagctc acgggtggtc agaagatcgt tcagttcaga catggaaaac
     3241 tccccagtgt tgcagcgatg ccagtgcctg ctgttcgagt tgttgatgat gtgcaatggc
     3301 ttgcggtgga caatgcaacg gcatttgcag tgccgaaacg ggaagttgcg ccagttgata
     3361 atgctcaagc acctgatgcg ccagcgtccc gcgttctcgt ggcggtagcc aggccattgc
     3421 cgcgcgtaaa tcgggcaggg cattggtaaa atcggcatca cttagttgat ttaaatgact
     3481 gctgaaaccg gcgataaatg ccggctgaca ggccagttga tgacgggcta atgccagcaa
     3541 accgtgcagc gcctcgccgg attgtgccgg ggataactgc gccagcatcg tcagcgccgc
     3601 ttcggcacta gcgttgggat gttccagacg catcagcgca ccaagagtcg cgccgcgatc
     3661 cagtgccagg acatgaatcg cctgactgtg tcgctccagc aaggctaccg ccgcagaaag
     3721 cgaaacgccg gggagttgca caccgctatg tagatcgcgc aggatatgac acaacgcttg
     3781 ccagctatgc aagtgagcgt gaaactcctt ttcatccggt ctgccgttgg attcacacag
     3841 ccacagcgtg cgatcgatag ccgcgcataa cgtcgtttgt aatatctgcg cgccttgcat
     3901 accgctaatt tcatccagcc gccataaggc atatagcact tccagcgcct ggcccatttc
     3961 ggcaaattga ttttcctggg cgattaataa tgtgagttgc tccagtaatt gttgactgaa
     4021 ggacgccaga cccgctgacg ccgcttggct aagacatgtg gtaatgctgc cgattccacc
     4081 cgcgtccagc atatcggctt ctaatttatg gcgtgcggct tcctggagtg tggcaccaaa
     4141 gcaggcggcc tcaatcaatg ccgcatgctg gctaagcggg cgggttaatt tccagcgttc
     4201 ttcaccgttg cctgcaagtg tcagtgtgct tccctgctgg cgtacaatcc ctgggatctc
     4261 cagtattgcc agccgatgta acacctgact ttgcgctagt ccattggggt taaagcgatt
     4321 taaggtaagc tccgccggaa gagagattgc ggtacgttcc agttcgcaag tgacatcttt
     4381 tggcagaggc ggttgtggcg tagaaggggc aagtttaccg aatccgtcac ccgctaatgt
     4441 gtctatcaac gttagcagaa tcggatcggt atcgggatga ataacgccgc gatagctcca
     4501 cggcaacggc gcattcaggg cttctttaat cagcgatcct gctatggcat ccagccagtc
     4561 agtgcgtaac ggtagtgtat gaccgcgcaa ctgtgccagt gccatcgcat gcagatgagc
     4621 ggcagccata tccgcggtcg aagcaggcaa cttgtgctgg cgcaaacggg tgagaaccgt
     4681 ttttagcagt tgttctcccg cctgctgtaa gccccactgc cagcaccagt tttgccagac
     4741 cggggcaggc attcctgaaa ggtatcctgc cagcacatca aggcgctttt cactgtaggg
     4801 ggtgagataa caacctgtaa cggcatctgc cagcgacggc aattctggcg tgttaattac
     4861 ctgcggacat tcgcgccaca tttttgccag tgccggagcg tgccagccac cgcagacaac
     4921 taacacgtcg ccattatttt gctgcatcgc ccatccaatc cagcgggcca taaaggcttc
     4981 gcgctgacga tttagagcat cgccggggga gtcgccccgc agttgggcaa aatagtgcgc
     5041 cagtgcagag ggtaatgcag tttgctggct ttcatcttcg aacaagtgat cccacagggt
     5101 gtcgctgtta tccatgcggg tggcacgcag cagcaaggtc tggctttcct cttgcgtatc
     5161 aggcgaatcg tcctcttctt cactctgcgc ccagcaaggc aaatcgatga agtaagtttg
     5221 tgcctgaata cgacgatcgg cttgtagcgc ctgccactcc ggcgaaaatt cagcaaatgg
     5281 cgtccaggca ccacgcccgg gggctgcacc gtcctgatac tggcaataac tgtaaatagc
     5341 taccggaagc tggtgggcta aaaacagttc gtctacccgg tcattaaaat cagcagggcc
     5401 ttcaatcaac acgtatcgtg gccgctggct ttcgattaac gatttcacca gccgggcgca
     5461 ggccggacta tgatgccgga tgccgacaat taacggctcg ctcatgcatc tttcctcacg
     5521 gcaggcggtg gcgggcttga taataagcct gccagtgagc ttctttatgt gtggcgacac
     5581 gctgttcgaa gtaacggcgc agacgagcgc gatcttcttc gttatctttg acgatggttc
     5641 cggcaatgca gtccaccagg tcggctggct cacctgcgcg attcgctaaa aaccaggcgc
     5701 ggacgcctac ggcgtgggcc acgttcacgg cttcagcggt ggacataatc gctgttagtg
     5761 tatccattga cgttttcttt tcgccgttgg cacgcagatc gcggaaggtg cggaccagta
     5821 actccagtac cgcgtcaggc actttatgcg gaataccgct atgggccagc aaacgcgccg
     5881 aagcactggc gacaagctcc agctcctggg cgaaatccat aatcggaaag acagtttcaa
     5941 aatcgaagcg acgctttagc gcagcactca tttcattgac gccgcgatcg cgggtattgg
     6001 cagtggcaat aatattaaag ccttctcgcg catagagctg actggcttca ccagtgagtt
     6061 ccggcaccgt catcaccctg tcagaaagca tacctaacag acagtcttgc acttccagcg
     6121 gtgtacgcgt tatctcttca aaacggacga ttttgccgtc gcgcattccc tggtaaagcg
     6181 gggcagggac cagcgcttcc gttgatggac catgattgat aagcagggcg tagttccagc
     6241 cgtacttgat ttgatcttcg gtagtagatg cgccgccctg gatggttaac ccggcatcgc
     6301 cgctaataga ggttgccagt aattcagaca ggagagattt tgcggtgcca ggttccccca
     6361 ccaacatcaa accgcgtccg gtggcaagag tgactaacat acgttcgaca gcggagggac
     6421 taatcaccac tttacggcta atattttgcg cgctatcgcc aaggataaaa gcacgggccg
     6481 caggcaaact taactgccaa ccgggcgggc aaggtgcgtt atcattttgt tttaattttg
     6541 ccagttcatc ggcgtataac acagcagcag gcggacgctg cagatgatta ttctgcggtg
     6601 acatatattc atccc
//
LOCUS       DADLCQ010000176         6405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000176 DADLCQ010000000
VERSION     DADLCQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.178"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQG95_003720"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="JQG95_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY5890135.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="JQG95_003721"
     CDS             complement(1433..1759)
                     /locus_tag="JQG95_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY5890136.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="JQG95_003722"
     CDS             1894..2235
                     /locus_tag="JQG95_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY5890137.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="JQG95_003723"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="JQG95_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="JQG95_003724"
     CDS             complement(2833..3543)
                     /locus_tag="JQG95_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY5890138.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="JQG95_003725"
     CDS             3651..3956
                     /locus_tag="JQG95_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY5890139.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVRHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5612
                     /locus_tag="JQG95_003726"
                     /pseudo
     CDS             4155..5612
                     /locus_tag="JQG95_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1708 a         1628 c         1501 g         1568 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actgggttgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcaggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatatataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct
     4441 gtttgcgcgg tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aatgacccaa
     4501 tgaaacgcgt gggtaaacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc
     4561 tggatactat cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaagctgtat
     4621 atattcagta ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct
     4681 cggcggtcaa acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca
     4741 gcaccgcgca aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca
     4801 ccacggatat tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc
     4861 gcatgagcgg tggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca
     4921 aaatgattgt tatcgatccg cgatataccg atacggctgc cggtcgtgaa gaggaatggc
     4981 tccctattcg cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta
     5041 acgaaaatct cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa
     5101 ccttacccgc agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg
     5161 acgataaaac agcaaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc
     5221 gtatcatcaa actggcgcgt gaaattggca caacaaaacc cgcttatatc tgctagggct
     5281 gggggccgca acgccaggca aacggcgagc tgactgcacg cgctattgct atgttaccta
     5341 ttttgacggg caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata
     5401 ccattacgat agaacgccta ccggtgctgg ataatccggt caaaacgtca atctcctgct
     5461 tcagctggac ggatgctatc gatcatggcc cgcaaatgac ggcactccgc gacggtgtcc
     5521 ggggcaaaga taaactggat gtgcccatta agttcatctg gaacttcaag gacttgaagc
     5581 ggacccttag taggcctgag gtcctgcatt gagtcaagac cttgtgtcat tctttgtgcc
     5641 agacttgtgt cattagacgg gatgtctaga cggctggtca gcccccccgg tggcctcggt
     5701 ctggacttga ggtgggccta tggggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggacttcag
     5941 gtctgcggaa gtcatagaca taaagcccct atctgttatg ttatattatt atattaacaa
     6001 caggtccaga ccattggtcc agaccatagt tagttaatca cagtgatagc ccaccacctg
     6061 aagtcctgct gatattcagt ccagacttac ctctgctcat ggtcttacct ccctcggtct
     6121 cctgaccttg ggtatcgact acagcttgct gagcgtcatc tggaggagct acaggaggcg
     6181 ggaccgaagc cccaccttga gtcccaccac cacctgtcag gcccttggcg atggacctga
     6241 agcctttact aatgctctta cccattggtc ttacgcttcg gtcttggtga acttcaggcg
     6301 tttgactaca ggcaggacgg cagtagctga gccagtgccc atcttgacgg tcgtagcagc
     6361 ttcagcgaca acgaacactt taccgaggat ttccaagtcg tggga
//
LOCUS       DADLCQ010000177         6375 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000177 DADLCQ010000000
VERSION     DADLCQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.179"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            59..1385
                     /gene="pgaC"
                     /locus_tag="JQG95_003727"
                     /pseudo
     CDS             59..1385
                     /gene="pgaC"
                     /locus_tag="JQG95_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
     gene            1387..1800
                     /gene="pgaD"
                     /locus_tag="JQG95_003728"
                     /pseudo
     CDS             1387..1800
                     /gene="pgaD"
                     /locus_tag="JQG95_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
     gene            complement(1850..2773)
                     /gene="phoH"
                     /locus_tag="JQG95_003729"
     CDS             complement(1850..2773)
                     /gene="phoH"
                     /locus_tag="JQG95_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY5890140.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="JQG95_003730"
     CDS             complement(3246..4511)
                     /gene="efeB"
                     /locus_tag="JQG95_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY5890141.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(4517..5644)
                     /locus_tag="JQG95_003731"
     CDS             complement(4517..5644)
                     /locus_tag="JQG95_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY5890142.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5702..>6375)
                     /locus_tag="JQG95_003732"
     CDS             complement(5702..>6375)
                     /locus_tag="JQG95_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY5890143.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1535 a         1680 c         1533 g         1627 t
ORIGIN      
        1 aaccaacctg aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat
       61 gattaatcgc atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt
      121 agcgtacttt cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat
      181 gtccattatg tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc
      241 gtggggagaa aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat
      301 tccctgtttt aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg
      361 ttatgagaac attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat
      421 cctggatcgc atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaaaca
      481 ggggaaagcc attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg
      541 cattgatggc gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt
      601 gtacaacccg cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct
      661 ggtgggtaaa attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca
      721 gcgtatctat ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc
      781 cctggcagaa gtgggttact ggaatgacga tatgatcacc gaagatattg atattagctg
      841 gaagctgcag ttgaatcagt ggacgatttt tttacgagcc acgggcgctg tgctggatat
      901 taatgcctga aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg
      961 cagaagtatt cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc
     1021 cgctgttttt tgaatactgc ctgacgacaa tatgggcctt cacctgcctg gtcggtttca
     1081 ttatttacgc agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg
     1141 cgacacatac tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc
     1201 tgatgatcga gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt
     1261 tcccggttat tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca
     1321 tgttgatgcc taaaaagcaa cgcgcccgtt gggtaagtcc tgatcgcggg attctgagag
     1381 gttaatatga acaatttaat tattacgacc cgacaatcac cagtacgttt actggttgat
     1441 tatgttgcca caaccatctt gtagacatta tttgctttgt tcatattctt attcgccatg
     1501 gatctgctga cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt
     1561 ttgctggcag tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt
     1621 tttcaaaaac agcagcatca tgcagcctac caatatacgc cgcaagaata tgcagagagc
     1681 ttagcaatac ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac
     1741 ttcaccagcc aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa
     1801 acacccaaaa caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg
     1861 catgcagcgt acgttggcaa agtgccgaac gtacgcaatc ctcttttccg aaccggacga
     1921 tcccaaccat ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcacacgc
     1981 cgcgaggcaa atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga
     2041 ggcgggttaa aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga
     2101 caactgcatt ttcaaaggta cgtccacgca tataggcgaa cggcgcggtt tccaccttcc
     2161 ctatttccgg tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcaagt
     2221 cgtagaccgg gcgaaaatag ggagcaaact tttctgcgat atcaccaggt aagaagccaa
     2281 gatcttcatc ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta
     2341 tcagggcctc tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg
     2401 tggcgaatat cagctgctta ctctcaatag cctgcagata gtgcaattgc gcttcatttc
     2461 gcgcaaggat gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc
     2521 tcatctgcac aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc
     2581 gtctcagcac acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg
     2641 agagcacctt gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac
     2701 gaacatcaga gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa
     2761 aacggctacg cacaccgttt gcggcgtcgg taacacgagt aaagaaagga agtgagaaaa
     2821 atacagagtg acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt
     2881 aacaaaacat tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc
     2941 actgccggga acttccgctg ctacttaata agtacaacag aactcgcatt cattgcaaca
     3001 atatattcac ttatatttaa cgataaacac ccatttcggt gacattaatt tctactggaa
     3061 agatgacaga gtgatgacag tgatggaaaa agctgtgtgc tttccgcagg attcgcaggg
     3121 ctggtaaaat gtgcctcgtg tttgccggat tcggcgcgaa cgccttaccc gacctacaag
     3181 tgattaataa tttgtgcaca aaactcatgc ccgataaacc tgcgcatcgg gcatatttgc
     3241 gtcgttcaaa cctgcaatag cgcccttccc agataatcgt tcgcgtcctt cactcccggc
     3301 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     3361 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     3421 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3481 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3541 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3601 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3661 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3721 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3781 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     3841 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     3901 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggtt caccgtgtcc
     3961 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     4021 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     4081 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     4141 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     4201 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca aacgaaacaa ccgctcaaga
     4261 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     4321 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     4381 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     4441 agcgcaccaa tccctttcag taaacgtcgg cgtgacggtt cattcacgcc gttttcatct
     4501 ttatactgca taacgcttaa tccagtccca gcacaccgcg aagttgcgcc agatcttccg
     4561 ccagcgcagt aatcggccct ttcagcgcat tccggtcggc atcggtcaat ttgtcgtagg
     4621 tttcaaaacc gtctttagta cggtatttcg ccagaatggt atcgaccttt ttaaagttgg
     4681 catcgacttt tgccaacagt tccgggttgg ctttttgcag ttgtggacgc agcagatcga
     4741 caattttctg cgagccttca acgttagcct ggaaatccca cagatcggtg tggctgtagc
     4801 gatcttcttc accgctgatt ttgctggctg ccacttcctc aatcagtccg gctgcgccgc
     4861 cgaccacttt tgaaggtggg aaagccagtt cactgatgcg tttttgcaaa tcgaccacat
     4921 cggtataaag ctgctcagcg tactgatcca tccctttggt ggtgttgtcg ccaaacaatg
     4981 ctttttccag acggtggaaa ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat
     5041 cttcacgggc gtcaatgctg ccatccagat cggagaacag ttcagcaatc ggttcaatac
     5101 gctcatagtg ctggcgcgtc ggcgcataca gtgctttcgc tttttcgata tcgcctgctt
     5161 taatcgcgtc ggtaaaggct ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat
     5221 acgctttata tgcagtaatt gcaccaccaa gacttaacag cgcatcactt tgcgccgcat
     5281 ccgccgttgc ctcacctttg acgatcaact tccctttcgg gttagtcagc agaccgcagg
     5341 tcatatcgta ttcgccaggc tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga
     5401 tattttcccg ctcttccacc accatcacgc ctttgaggat ctcccactcc agcgccttct
     5461 ggctatggtt ctgaataatg aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt
     5521 cgcactgctt atccgtcacg gtcactttga cctgcggcac atcagcggcg ttagccataa
     5581 aagcagaaga aaacagcgca gccacgctca actgcaatgc gttacggcgg aagttaatgg
     5641 tcatgacgag actatccctt taaagtatgt tgtaactaag caagagtttg cgtcgtattt
     5701 gttacgcgga gcgagacgct gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg
     5761 ccgggatgag ataaataaac cagacggcga cttcgctgac gctcggtgct tcctgatagc
     5821 caaaaatccc ttccatcagc gtgccaaaca gcgagtgagt tgagaggacc gcgctcatat
     5881 cgaaggcgat ttcctgaaag tggttccaca atccggcttc atgaaatgcg cgaatggcac
     5941 cagccgccag ccctgcggcg acgaagagaa taaacaggct ggtccattta aaaaatgcac
     6001 caagattgag gcgaataccg ccccagtaga gcaggaagcc gagcaccacg gcagtagcaa
     6061 gaccgagcat tgcacccagc ggcggccaga tcccgacatc ttgttgaaat gccgccagca
     6121 ggaaaaagac cgactccagc ccttcccttg caacggcaaa aaagaccatc atcaccagcg
     6181 cccagccatg atgatttcca cgctgcaatg cgctatcgac tgcctgttcc agttgcactt
     6241 tgacgttgcg cgacactttg cgcatccaga aaaccatcca ggtaaggatc accacggcga
     6301 tcaccgccac gataccttca aacagttcct gctctttttg cggaaattcg ccggtggttt
     6361 cgttaatgaa gatcc
//
LOCUS       DADLCQ010000178         6353 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000178 DADLCQ010000000
VERSION     DADLCQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.180"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..369
                     /locus_tag="JQG95_003733"
     CDS             <1..369
                     /locus_tag="JQG95_003733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890144.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="JQG95_003734"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="JQG95_003734"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY5890145.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="JQG95_003735"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="JQG95_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY5890146.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /locus_tag="JQG95_003736"
     CDS             complement(1516..2565)
                     /locus_tag="JQG95_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY5890147.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="JQG95_003737"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="JQG95_003737"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY5890148.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="JQG95_003738"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="JQG95_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY5890149.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6275)
                     /locus_tag="JQG95_003739"
     CDS             complement(5094..6275)
                     /locus_tag="JQG95_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAY5890150.1"
                     /translation="MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLI
                     LLVAWYGIRRMLLTPLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLT
                     DTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADN
                     ARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILAL
                     NAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRIDTGSVLVESA
                     GETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAA
                     AAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWET
                     F"
BASE COUNT         1439 a         1836 c         1525 g         1553 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgatc cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaggacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgcctggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agt
//
LOCUS       DADLCQ010000179         6335 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000179 DADLCQ010000000
VERSION     DADLCQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.181"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            254..1642
                     /locus_tag="JQG95_003740"
     CDS             254..1642
                     /locus_tag="JQG95_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAY5890151.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1724..5464
                     /locus_tag="JQG95_003741"
                     /pseudo
     CDS             1724..5464
                     /locus_tag="JQG95_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            5461..>6335
                     /gene="narH"
                     /locus_tag="JQG95_003742"
     CDS             5461..>6335
                     /gene="narH"
                     /locus_tag="JQG95_003742"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY5890152.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1492 a         1685 c         1663 g         1495 t
ORIGIN      
        1 attcacgatc tgaagggttg aaacatgaca tacgttcaaa gattaacccc cgtttgaagg
       61 ttagcttgtt agttacattt agtaacacat atctggctac ttaatcccca ttctgccccc
      121 gcggttacgc tttcttatct attctttcaa tcagcacttc acccaattca tccttaattc
      181 taataactgg ctattgcgcc ttacctcctg ttttgtcttt tttcattacc aatgtgtgca
      241 tatgaggaac aatatggcac tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg
      301 gaaaccagaa aatccggcct tctgggaaaa taaaggaaaa catattgctc gaagaaacct
      361 ctggatatca gtcagttgtc tacttcttgc cttctgtgtc tggatgctat ttagcgcagt
      421 taccgttaat ctcaataaaa tcggttttaa ttttactacc gatcaactct ttttattaac
      481 cgcattaccc tccgtttctg gcgcattatt gcgtgttccc tactccttta tggtgcctat
      541 attcggtgga cgccgatggc cggtttttag tactgcaatc ctgattattc cttgcgtctg
      601 gctcggaatt gccgtgcaaa atccgaatac tccttttggg atatttatcg ttatcgcttt
      661 gctatgcggt tttgcaggtg caaactttgc ttcgagcatg ggcaatatca gtttcttctt
      721 tccaaaagcc aaacaaggga gcgctcttgg gattaatggc ggattaggaa acttaggtgt
      781 aagtgtaatg cagctggttg caccgctggt catttttgta cctgtatttg cctttctcgg
      841 cgtcaatggc gtaccgcagg ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat
      901 ttgggtaccg ctactggcga ttgccacgat cgccgcctgg tcagggatga atgatatcgc
      961 cagttcacgc gcctcaattg ccgaccagct ccctgtctta caacgcctgc atctctggct
     1021 gctgagcctg ctttaccttg ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc
     1081 catgctggca aaaacccagt tcccggatgt gaatattctg cgcctggcgt tctttggccc
     1141 atttattggt gccatcgcgc ggtcggttgg tggtgctatt tccgataaat tcggcggcgt
     1201 gcgggtgacg ttgatcaact ttatttttat ggcgattttc agtgccctgc tgttccttac
     1261 cttaccgggc acaggctccg gtaatttcat cgccttttac gccgtattta tggggctgtt
     1321 tctgaccgcg ggtctgggaa gtggttctac tttccagatg atcgccgtca tctttcgcca
     1381 gataaccatt tatcgggtga agatgaaagg cggtagtgat gagcaagctc agagagaagc
     1441 cgtcaccgaa acggcggcaa cgctgggctt tatctcagcc attggcgcag tgggcggctt
     1501 ttttattccg caggcatttg gcatgtcgct caatatgacc ggctctccgg tcggcgcgat
     1561 gaaagtgttt ttaatcttct acatcgtttg tgtgctgctg acctggctgg tttatggtcg
     1621 gcggaagttc agccaaaaat aagagatcga tgcctgatgt acaaccatgt cgcatccgac
     1681 aaggcatcag atcccaattc attcctggag caggagtcat gtcatgagta aacttttgga
     1741 tcgctttcgc tacttcaaac aaaagggcga aacctttgcc gatggtcacg gacaggtgat
     1801 gcatagcaac cgcgactggg aggacagcta tcgccagcgt tggcagttcg acaaaatcgt
     1861 gcgttccacc cacggcgtta actgtacagg ctcctgtagc tggaaaatct acgttaaaaa
     1921 tggtctggtg acctgggaaa tccaacagac cgactacccg cgcactcgcc ctgacctgcc
     1981 caatcatgaa cctcgcggct gcccgcgtgg cgcaagttac tcctggtatc tttacagcgc
     2041 taaccgcctg aaatacccgc tcattcgtaa acgactgatt gaactgtggc gcgaagccct
     2101 caaacaacac agcgatccgg tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg
     2161 cctgagctac aaacaagtgc gtgggcgcgg cgggtttatc cgctccaact ggcaggaact
     2221 aaaccagctg attgccgccg ctaacgtctg gaccatcaaa acctacggcc cggatcgcgt
     2281 tgccggtttc tcgccgatcc cggcgatgtc gatggtttct tacgccgccg gaacgcgtta
     2341 tctgtcgctg cttggcggca cctgttaaag tttctacgac tggtattgcg acctgccgcc
     2401 cgcctcgccg atgacctggg gcgagcaaac cgacgtaccg gaatctgccg actggtataa
     2461 ctccagctac atcatcgctt gggggtctaa cgtaccgcag acacgtacgc cggacgccca
     2521 cttctttacc gaagtacgct acaaaggcac taaaaccatc gccattaccc ctgactactc
     2581 tgaagtggcc aaattgtgcg accagtggct ggcaccgaaa caaggcactg atagcgccct
     2641 ggcgatggca atggggcatg tgattttaaa agagtttcat ctcgataatc ccagcgacta
     2701 ctttatcaac tactgccgcc gctacagcga catgccgatg ctggtaatgc tggagcctcg
     2761 cgacgatggt agctacgttc ccgggcgcat gatccgcgca tctgacctgg tggatggact
     2821 gggcgaaagc aacaatccgc agtggaaaac cgtagcagtt aataccgcag gtgaattggt
     2881 agtgccgaac ggttcgattg gtttccgctg gggagaaaaa ggcaaatgga atctggaatc
     2941 cattgccgcc ggtacggaaa ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt
     3001 tgcaggcgtg gccttcccct actttggcgg cattgaaaat ccgcattttc gcagcgtaaa
     3061 acacaatccg gtgctggtgc gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa
     3121 cacctgtccg gtggtcagcg tttatgattt ggtactggcg aattacggcc tcgatcgcgg
     3181 gctggaagat gaaaacagcg cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg
     3241 gggtgagcaa attaccggcg taccgcgcca gtatattgaa accatcgccc gcgaatttgc
     3301 cgataccgcc cataaaacgc atgggcgctc aatgattatc ctcggcgcag gtgttaacca
     3361 ctggtatcac atggacatga actaccgcgg gatgatcaat atgctgatct tctgcggttg
     3421 tgtcgggcaa agcggtggcg gctgggcaca ctatgtcggc caggaaaaac tgcgcccaca
     3481 aaccggctgg ttgccactgg cctttgcgct cgactggaac cgaccaccgc gccagatgaa
     3541 cagcacctcg tttttctaca atcattccag ccaatggcgc tatgaaaaag tctctgctca
     3601 ggagttactt tcaccgctcg ccgatgccag taagtacagc ggtcatctga ttgatttcaa
     3661 cgttcgcgcc gaacgtatgg gctggctacc ttctgcgccg cagctggggc gtaacccgct
     3721 cgggattaaa gctgaagccg acaaggccgg attatccccc acagaattta ccgcccaggc
     3781 gctgaaatcg ggcgatttac gtatggcctg cgaacaacca gatagcggca gcaatcatcc
     3841 gcgtaatttg tttgtctggc gctctaacct gcttggctcc tccggcaaag gccacgagta
     3901 tatgcagaag tatctgctgg ggaccgaaag cgggattcag ggcgaggaac tcggtgccag
     3961 cgacgggatc aaaccggaag aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga
     4021 cctgctggtg acgctcgact tccgcatgtc cagtacctgc ctgttctccg atatcgttct
     4081 gcccaccgcc acctggtatg aaaaagacga tatgaacacc tcggatatgc atccatttat
     4141 tcatccgctt tctgcggcgg tcgatccggc ctgggaatca cgcagcgact gggaaatcta
     4201 caaaggtatt gccaaagcat tttcgcaagt gtgcgtgggc catcttggca aagaaaccga
     4261 cgtggtatta caaccactgc tgcatgactc tccggcagag ctctcacagc cgtgtgaagt
     4321 gctcgactgg cgcaaaggcg aatgcgatct gatcccgggt aaaaccgcgc cgaatattgt
     4381 ggcggtggag cgcgactacc ctgctacgta tgaacgcttt acctcgctcg ggccattgat
     4441 ggacaaactt ggcaatggcg gtaaagggat ttcgtggaat acccaggatg aaatcgattt
     4501 cctcggcaaa ctcaattaca ccaagcgtga tggcccggca caggggcgtc cgctgattga
     4561 caccgccatt gacgcttcag aagtgattct ggcactggca ccggaaacca acggtcatgt
     4621 tgcagtcaaa gcgtggcagg cgctgggcga gatcaccggg cgcgaacata cccatctggc
     4681 gctgcacaaa gaggacgaga agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat
     4741 tatctccagc cccacatggt ccggtctgga aagcgatcac gtatcctata acgcgggata
     4801 caccaacgtt catgagttaa ttccgtggcg cacgctgtcg ggacgccagc agctctatca
     4861 ggatcatccg tggatgcgtg cttttggtga aagcctggtg gcttatcgcc cgcctatcga
     4921 cacccgtagc gtcagtgaga tgcgccagat accgccaaac ggcttccagg aaaaagcact
     4981 taacttcctg acgccgcacc agaaatgggg cattcactca acctacagtg aaaacctgct
     5041 aatgctgacg ctctctcgcg gtggaccgat tgtctggatc agcgaaacag atgcccgaga
     5101 actgaccatt gtcgataacg actgggtgga agtatttaac gccaacggcg cgctgactgc
     5161 ccgcgcggtg gtcagccaac gtgtaccgcc gggcatgacc atgatgtatc acgcccagga
     5221 acgcattatg aatattcctg gttcggaagt aactggcatg cgcggcggca ttcataactc
     5281 ggtcacccgc gtttgcccga aaccgacgca tatgattggc ggttacgcgc agctggcctg
     5341 gggctttaac tactacggca ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa
     5401 gatgaagaac gttaactggc tggatgatga aggtcgcgat caggtacagg aggcgaaaaa
     5461 atgaaaatac gttcgcaagt cggcatggtg cttaacctcg ataaatgtat cggctgccat
     5521 acctgttcgg tgacctgtaa caacgtctgg accggacgcg aaggcatgga gtacgcatgg
     5581 tttaacaacg tcgaaaccaa accgggcatt ggttatccga aaaactggga agatcaggaa
     5641 gagtggcaag gcggctgggt ccgcgatgtg aatggcaaga tccgtccgcg tctgggcagc
     5701 aagatgggcg taataaccaa aatcttcgcc aacccggtgg tgccgcagca gattgatgat
     5761 tactacgaac ctttcacctt cgactacgaa catttgcata gcacaccgga aggcaaacat
     5821 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     5881 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5941 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6001 ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg cgacctgccc aagcggcgct
     6061 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6121 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6181 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6241 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6301 gaggaagcgt cgagcaccga gcgcgaagtt gacct
//
LOCUS       DADLCQ010000180         6268 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000180 DADLCQ010000000
VERSION     DADLCQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.182"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..666)
                     /locus_tag="JQG95_003743"
     CDS             complement(<1..666)
                     /locus_tag="JQG95_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HAY5890153.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..2413)
                     /locus_tag="JQG95_003744"
     CDS             complement(656..2413)
                     /locus_tag="JQG95_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY5890154.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAVSDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDVWGIFTVSMAVRRDN"
     gene            complement(2429..3313)
                     /locus_tag="JQG95_003745"
     CDS             complement(2429..3313)
                     /locus_tag="JQG95_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY5890155.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(3324..3929)
                     /locus_tag="JQG95_003746"
     CDS             complement(3324..3929)
                     /locus_tag="JQG95_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY5890156.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILFICTAINRCRSVLLMSAER"
     gene            complement(4100..>6268)
                     /locus_tag="JQG95_003747"
     CDS             complement(4100..>6268)
                     /locus_tag="JQG95_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HAY5890157.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1835 a         1568 c         1316 g         1549 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 ttacccacgg tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg
      841 ggtgccacgg ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggctagac
      901 ctgcgagtga gttttttacc tgctcatttt cgggaaaaag ctcatatagg ccagagacaa
      961 tcgccagcgt aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct
     1021 gttcaaaacg cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa
     1081 catttaactc actgtaatca cgtaacaaaa tatcgcttac ggcaggctcg tttgccagcg
     1141 catccagtac atagcgtcca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt
     1201 tggcgtggag atcggcaacg gcttgtttaa tcaatatttg caaatgggtt ttgcgctggc
     1261 gaataccgcg ccatccaaca ctgttcaggt agattttgtc gattaaacgc ccgaatgcgt
     1321 tactgccttg cggttgattt tgatagacat aatccaggga actgcctgaa tcaaagccgg
     1381 tactcattcc gagatgcagg cccgcagagt gcgcaccgaa caatttcatc gcctttcgca
     1441 taaagcgata cgctaaatca accggcgata atggcacggg tccaccagaa aggagccgcc
     1501 atcgatccgc tgatggtcct gtgcggtctt catgctgata atcaaatttt tgcgatttgt
     1561 tagcatataa gcggcagata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt
     1621 cacccaatgt gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta
     1681 atctctgata aaaatcaatc tgcggttggc gatgcaccac atagtcatca cctgatatca
     1741 gaagttgcgt ggggagcgta atcgccgccg catcgctaac aatacgttca gacgttttat
     1801 agagatcgag caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac
     1861 tcgccacccg ttgccgatcg tgggtcaaat agcgcccttt cacataggaa ttaataaaaa
     1921 atagaccacg cagacgatgc cataacgcca gcgcaggacg tgccagcggc acatacaatt
     1981 taaccttaaa ggccggagaa gccagcacca gcccacgaat tgcaggtgca taatcatgaa
     2041 cccatgtggc aacaagcact gcgccgacgc tttgcgcgat cactaccacc tcttccagtc
     2101 cgacctggct gtcgctgaca gcaaaacgga caaattcatc gacatcctgc actgagcgca
     2161 taagagacgg gctataacca cgcggccccg aattttttcc atgcccccgg gcatcccagg
     2221 cataaaaagc agtatcaggc atcgccagtt catcaacgag atgttgtaga cgaccagaat
     2281 gttcatgccc gcgatgaaat aagacgatga cctttttcgc ccctggctgt aaagtgggcc
     2341 agtgccgata atacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagggatgc
     2401 gtgaattttc cattttctct attttccttc agtaacagca gtagcgaata aaataaaaaa
     2461 ataccggagc ggtgaaaatt aatgagtcaa tcctgtcaag gagtccgcca tgtcctagta
     2521 atagttttcc actatcttta acaccaatat ctcgtttgat ggctgacatc actacatcgc
     2581 cgcaaaaacc actaatacca attaacaaac ccgctaataa tgcctgtaat gtatttagcg
     2641 gtgtcagcaa cggtccaata atcagtgacg caatcatggt ggtgattacg ccccccagca
     2701 gaccttccag cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt
     2761 tcccccataa atactgtgca atatcgtttg actcggttaa cgccaaaaga aataacacca
     2821 gtaacgcccc gccctggata tttatggttg gcaacattaa tagccaggcg gcatgactca
     2881 aggcaaaaac ggtcattatc catccccaaa aaatggcgct cacggtatgc tgaaaaccgg
     2941 aggggtcacc cacaaaaact cgccaggtgg ctaatatcag aaacccagtc aaaggtataa
     3001 ataacagaaa cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc
     3061 aatataacca acgcgaaaaa tgtacggata taagcgtaca atattctttc aatgccagaa
     3121 aacttatcag cgtaaaaaac gtcaacgtca tccagcgtgg gcctgaaatc accagtgaaa
     3181 aacaaatgac gatgccccac caggtacgaa tacgtaatgt aacttcatcc ccttttctct
     3241 caggtaaccg ccatagcaga aaacggttta tcagcttgct aaaattaaca acgcgaaaag
     3301 tgttgccaga gatttttcca gcattatctt tcagcgctca taagaagcac actccgacaa
     3361 cgattaatcg cagtacagat aaacagaatt gaagcaatgc tccataaaag attattccat
     3421 tgcatccact gtggataaat ggcaacggcc agaccccaca gaccaaatat taacgcgcga
     3481 tcacttttgc caaatggacc ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa
     3541 ccgcaaaact cggtcaatat ggtgcaaaat aacatgagta tcacgagtga tgcgttactt
     3601 tccggtaaaa ataaaaacgg taaatagagc gcaatatcgg aaataacatc gccagtctca
     3661 ttcaaaatcg cccccagacg tgtttgctga ttgcactcac gcgccaacat gccatccagc
     3721 gcattgagcg ccatacggat aaaaagcacg atgggcaata gcaaaaagag gatgggttgt
     3781 gccaccaaca ccaacagcaa tccggtaaaa agagaaagcg ccagtgccgt aagagtgatg
     3841 tgattcgctg taacgtggtg cttataaagc caaaacatcg tcggccttaa cagcgactga
     3901 aagagcggtt ttatctggta gagcgtcatt atcaatcctt gataattcgg tttgcgctat
     3961 ggtgcatcaa ttcccctgac ggagatatcg caacacgccg cttcattaaa aattcagaat
     4021 atatcaaggt attgggggct cactgtcagg aatgattatc cccaataaat caagtagtta
     4081 aaaattatta cgctgttatt caatacaact catcaagttt cctcatcgct tgttcccgta
     4141 ttgcgcgcgt cttaccgtta agcgccagct ggcggactaa atccagttca gggttaacaa
     4201 gctctaacgc cagcaaagga tcacgtttca gcctcgcctt caatacatca actggaaagc
     4261 ggggattatt catcgccaca atccaccact cgggatcaat ttctgccgcc agtgccgcag
     4321 agggggtgtg gcggttgcta agtgcgccga acttgaaaaa tgtgagattg cttttcgcca
     4381 gtgtactcca ccatatacga tccggttgtt ttgccagtac atccagttgt gctaacgcat
     4441 cggggtcgag tgcataataa acacgtaatg cttcatcaac ctgcgcacgg gagctatttt
     4501 cctgcctcag aaaaaacttc gcccaaatat cattgcgtgg cgtgttttgc tgtaattcct
     4561 tgagaacaac agcgggtaaa ttatcccaac taactaatgc actctctaaa aagtcccagt
     4621 cttccaaacg tatcagggcg gcggcattta atagcatctg gtcatcaact tcgccgtggc
     4681 taatcagctc agacaaaatc atatcagcgc tggtttcata tgcatctgaa tctgctttcg
     4741 gattattttt ccgatgcgat aaagcacgtt gccaaatttt ttgtttcatc gacgtgctca
     4801 gatactgatt tttcgccaga ttactccacc cctcaggatc gttgaaacgt gtcaataacc
     4861 gttgtaaagc agcgtcagag tgaattacct gcatatcccg acagcaaaag taggcagggg
     4921 attttttctc catcctttta acaaaattaa actgtaagtt atctggcaat gctaaaaata
     4981 acacacgtat tagatcatcg tcattctcga atttttggta taaataggct gcaactttgt
     5041 cactctcttg tggcgtcatc cgcaataatt gaccatggtg tttcatctct gccagcttga
     5101 acgctaagct tgtgacaaca ttgtttgatg aatcgttcgc caactgcatt gcctgattgt
     5161 aagtgagttg tcctccagca ccgacctgac gaatagagga atcatcactg cggatatagc
     5221 catcaataat ggattcgggc aataaccacg catctttata aagctcctga atgatcatat
     5281 cccaatctct tttcgccaga gtcatgacga tatccagagg gagattttca cgtagcgcaa
     5341 gattcaacgg accgacacca tcaggtagac gactcattcg cgtcattatt tcgacagaga
     5401 gcggttcacg gtcgagcaac cccagaacaa caggctggta acccaattgc tgatcggtca
     5461 actccagaat ctcattgtca ggcatatatc tcgccatact ttcaatcgtt tgttcatcat
     5521 tgctattaat aagttgtttg cgtaggttag ctgaaattcc tttaagcctt gggagagttc
     5581 gcattatggc aagatcaaat gtcgcaatat ctcgtccttc aaatgcagca acgttgcctt
     5641 catccagcat ttgcaattcc tgcactaact caggagaagg cttagggcga aacagcgctt
     5701 cttttttcat ctgattacag acattccctg ccatgcgaat attgcttcgc ttaaaaattt
     5761 ctttcagagc atcttcatca tttattagac cccaataata tgcatcagga tttattatca
     5821 tcagcgcttt tttgacattt tcagtgtcat ttttgtcact cgcacggacc atgaattcac
     5881 tatttgtcga agacgcaaca acaggtgcaa ttatgtcatt gataaatacc ccacgcgcat
     5941 taatactccc cttaatcgta gacggattat cgtaatatga gtcgataaat accagtccac
     6001 ttgcatttat cgaaccggta attaacagtg gtgaatccgt aactatattc gttgccgtca
     6061 cgttaccgag aataacccat ggattagaaa gagagaaagt ggaaatatta cctttgactg
     6121 tcaaattgcc aagaacaatc aatttttgat tactctctat attaccttct accactaaat
     6181 cgccacgatg cactttccag tccgatgcag ataaaccgaa ggggacaaga ttcacgctac
     6241 tgttgatagc ttgttcaact tgttgttt
//
LOCUS       DADLCQ010000181         6252 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000181 DADLCQ010000000
VERSION     DADLCQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.183"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JQG95_003748"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JQG95_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY5890158.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JQG95_003749"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JQG95_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY5890159.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JQG95_003750"
                     /pseudo
     CDS             complement(2659..5772)
                     /locus_tag="JQG95_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(5797..6054)
                     /locus_tag="JQG95_003751"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="JQG95_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT         1656 a         1456 c         1476 g         1664 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attatcttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag at
//
LOCUS       DADLCQ010000182         6145 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000182 DADLCQ010000000
VERSION     DADLCQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.184"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            6..311
                     /locus_tag="JQG95_003752"
     CDS             6..311
                     /locus_tag="JQG95_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890160.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(387..851)
                     /locus_tag="JQG95_003753"
     CDS             complement(387..851)
                     /locus_tag="JQG95_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY5890161.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(848..1723)
                     /locus_tag="JQG95_003754"
     CDS             complement(848..1723)
                     /locus_tag="JQG95_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY5890162.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1720..2409)
                     /locus_tag="JQG95_003755"
     CDS             complement(1720..2409)
                     /locus_tag="JQG95_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY5890163.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="JQG95_003756"
     CDS             complement(2457..3947)
                     /gene="nanT"
                     /locus_tag="JQG95_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY5890164.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="JQG95_003757"
     CDS             complement(4057..4950)
                     /gene="nanA"
                     /locus_tag="JQG95_003757"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY5890165.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="JQG95_003758"
     CDS             complement(5072..5863)
                     /gene="nanR"
                     /locus_tag="JQG95_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY5890166.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1550 a         1674 c         1534 g         1387 t
ORIGIN      
        1 ttgccatgat ttacattgaa cagcttgaat taatccataa aagtggtgat gtgttgtatc
       61 cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt ccaccttgtt ccattcggct
      121 taaataagac tcacgacctt ctagaggtgg aagatgcttc agaagcaatt cggctggtga
      181 ttgatgaacg tcattccatt cgctgcagta cacttacagc aactattacg aataaaaagg
      241 gcaagcgaat taagcggaca ggtatataca gtatcaaggg tgttaacata aaagaataca
      301 acgtcaggta atggcacaga gcggtccgtc agttcaggtt tggctctgtg ccaaagatgg
      361 gtcaggtcac acctgatcta ataaatttaa gccattaaat cagccttaac cttcaccacc
      421 acctttttaa tctcacccgg ctcgccaaca acgcatcccg gcttatgcgg ctctcctggc
      481 ataaacacgg cgaacattcc cggtttcagg atgatggttt gctcgttttc aatggcgcta
      541 caaagctggt aatcatcttc atggtggaac tcttcacatt ggcgcgcagt gcctgccatg
      601 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgctcgtgc
      661 aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaaaata
      721 ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc agctaaggcc
      781 agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca
      841 cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcat
      901 ggcggtaatg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagataca
      961 tttccaccaa cgccagatac ccttctgcca gaccaacgct gccaccaacc accacgcact
     1021 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcgat
     1081 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg tgtgaaaata gttctcgcat
     1141 ccgcgccagc cagctcgccc tgcgctgccg ccgcaatgcc gcgaccagaa gcaattgctt
     1201 ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg tggatcggca agcgtatgcc
     1261 cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc actacaccgc
     1321 cgccgacgcc agtggaaacg gtgataaaga ccatatcggt tatatcgcct tccagcgcct
     1381 gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa
     1441 gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta tgcggattca
     1501 gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
     1561 gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct ggtgtctggc
     1621 tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg
     1681 cggcaagttt agtaccgccg atatcaatcg ccagtatggt catagcaccg cctttttcat
     1741 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
     1801 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
     1861 ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc agcgccagat ccggctcttc
     1921 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
     1981 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
     2041 atggatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat
     2101 ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca tcttcaatat aggccgtgat
     2161 gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac
     2221 cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga atggcaaccg cgcccgcctg
     2281 ctctgccgct aatgccatgg ctgcgacgat ttcgggttta tcgagcgggc tgtcaggaac
     2341 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
     2401 taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca acaggattaa
     2461 cttttggttt tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt
     2521 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg
     2581 gaaggcatat ccagaccaat cagcaggatc accacaaatg tcaggctgaa cgagagcgat
     2641 gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt
     2701 gcgccaccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta
     2761 tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc
     2821 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
     2881 ataatcagca gctgcgaggc cagcaggcta caaacgtacg ctttgcgggt tcccagccag
     2941 tcaccgagga agccacctac gcagcatccc acggccgcgc caaagccact aaagaacagc
     3001 acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc
     3061 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
     3121 gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac
     3181 agcccgagaa cagcgacgat tgcagcattt tgcaggttac cggcgaagca gaaccacagc
     3241 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
     3301 atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt tctctttcca gtcttccgct
     3361 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
     3421 agcgcacgcc aaccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
     3481 cccacagaga agcctgaaat caaaaaacca ctggctttgt tacgcagatg ttttggccag
     3541 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
     3601 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
     3661 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
     3721 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
     3781 tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
     3841 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcgcg ccattgtgca
     3901 cggttgagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
     3961 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
     4021 ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt gcatcaactg
     4081 ctgggccagc gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg
     4141 gcacagcggc acagaaacga catccatata atgcaggaca gttttcaggc cgcggaatac
     4201 gcccgttttg atcagtaaat caatgacttt gttgcactca gtttgcagtt tctgcgcggt
     4261 ctggatatcg ccttctttca gcgccttcac gatcccctga tatcgccagc ccatgatgtt
     4321 gtaagtactg ccgataccac catcagcgcc tgccagcaag ccggaagcga agatttcgtc
     4381 gtaaccgtta tagagcacca gatcaggatg ttcacgacgg atctgttcca tctgatagag
     4441 atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg
     4501 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
     4561 atccgctgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
     4621 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
     4681 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
     4741 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
     4801 ttcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
     4861 caggcggcgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc
     4921 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
     4981 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
     5041 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
     5101 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
     5161 caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt
     5221 tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca
     5281 gggcaacgtg gatcgccatg aagattgggt taccggggat ctccgccagc acgcggtgga
     5341 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttgatttcca
     5401 gtgcttttgc cagcaaatcg atttgctcat cggtggcgtg ttcagccgca tagcgcacca
     5461 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
     5521 ggaaatcttt cgccatgccg gaaagctcgc cgatgatagt gtccgcagaa ggtcgagaga
     5581 cacgagcgcg ttcgccgttg tttatttgca ccagaccttt gcgttttaac gctgccagcg
     5641 cttcacgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
     5701 attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
     5761 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
     5821 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
     5881 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
     5941 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
     6001 tgtaacgcag ataaacacat cccgattgaa ttattcataa gactattcac aagcattatg
     6061 aatattatga atgtgttctt acaaaataat cataatcgca tattttttaa tgaaaaatca
     6121 cctcacctac aattaaaaac acgac
//
LOCUS       DADLCQ010000183         6128 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000183 DADLCQ010000000
VERSION     DADLCQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.185"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..361)
                     /locus_tag="JQG95_003759"
                     /pseudo
     CDS             complement(1..361)
                     /locus_tag="JQG95_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="JQG95_003760"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="JQG95_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="JQG95_003761"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="JQG95_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /locus_tag="JQG95_003762"
     CDS             complement(1781..2347)
                     /locus_tag="JQG95_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890167.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="JQG95_003763"
     CDS             complement(2629..2943)
                     /locus_tag="JQG95_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890168.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="JQG95_003764"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="JQG95_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="JQG95_003765"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="JQG95_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY5890169.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="JQG95_003766"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="JQG95_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="JQG95_003767"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="JQG95_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5609..5768)
                     /locus_tag="JQG95_003768"
                     /pseudo
     CDS             complement(5609..5768)
                     /locus_tag="JQG95_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5772..6128)
                     /locus_tag="JQG95_003769"
                     /pseudo
     CDS             complement(5772..6128)
                     /locus_tag="JQG95_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1650 a         1352 c         1243 g         1883 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacggccagt atcgcgaact ccggcaccgt
      181 tgaaagccat ttcagtgatt tggtttttaa ctcccggttt acgggcctcg taacagtaag
      241 tgagctgaaa cacccggtga cagtcacggc aacggaatcg atcatgtcct tttgggttct
      301 gcccgtgacg gtaaacctga acggactggc aacgggggca atgaaagtta actgtgacca
      361 tgaaagaacc taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaagt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtaagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gagcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggc gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataagatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgcga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcca
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcaccttg tcatgtaaaa tagtaacatt gtcattattt tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgacg ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttggctggg acgtagccgc tgacagcttc tataaaagta tgatcctacc
     3001 cacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatata attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agagggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc tctatcacag
     3241 tgcactttac ttaatagggt gggatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgttct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attatttttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataacctt tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tgtccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacaaat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagca
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt
     5401 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     5461 aattacacca taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc
     5521 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     5581 cccacataat atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga
     5641 gaccaccaca ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca
     5701 ctgttgcccg cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc
     5761 agcgaatgaa ttttcatctg ttgctgtatc cagtcataca gtgactgcat cagtggcgcg
     5821 gctctggctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5881 atggcataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5941 gcatgcacat cgtggatttt tctccggaca tgagccatac atgcggcttc cgttattctg
     6001 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     6061 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     6121 gccggagg
//
LOCUS       DADLCQ010000184         6111 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000184 DADLCQ010000000
VERSION     DADLCQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.186"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..332)
                     /locus_tag="JQG95_003770"
     CDS             complement(<1..332)
                     /locus_tag="JQG95_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890170.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFP"
     gene            complement(329..2557)
                     /gene="crfC"
                     /locus_tag="JQG95_003771"
     CDS             complement(329..2557)
                     /gene="crfC"
                     /locus_tag="JQG95_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY5890171.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2959..3294
                     /locus_tag="JQG95_003772"
     CDS             2959..3294
                     /locus_tag="JQG95_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY5890172.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3452..3895
                     /gene="yjdN"
                     /locus_tag="JQG95_003773"
     CDS             3452..3895
                     /gene="yjdN"
                     /locus_tag="JQG95_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY5890173.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            4028..4816
                     /gene="phnC"
                     /locus_tag="JQG95_003774"
     CDS             4028..4816
                     /gene="phnC"
                     /locus_tag="JQG95_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY5890174.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            4841..5857
                     /gene="phnD"
                     /locus_tag="JQG95_003775"
     CDS             4841..5857
                     /gene="phnD"
                     /locus_tag="JQG95_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5890175.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            5963..>6111
                     /locus_tag="JQG95_003776"
     CDS             5963..>6111
                     /locus_tag="JQG95_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HAY5890176.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         1542 a         1650 c         1522 g         1397 t
ORIGIN      
        1 tccgggaaag cagagtgctg gcggtgatcc attttttgca gcagcgccag atgctgcgcg
       61 atctgcgtca gcgccagatg accgggatcc agcatcgacg ccagtttttc caccattgtc
      121 agtcgcaggc taagcggcgc atttaacatg ccgccgttcg tccatgtctg aagctgcaca
      181 cgcgaaagaa gttcctgcat tagtcgttca cgaaactgct gctgatgggc ttgaggatgg
      241 cgggcatcat cgccctgcgc cagatccact aaaaagcgcg gatgaaccga ctccagcacg
      301 cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa
      361 tatcatcgcg taacagctga gcgtcggtgt aaatccagct gtgagtcatc acagtctgct
      421 gcaattgctg ctgcatcagc ctgaccaccg attcattttg ttgacgcaga gccagacttt
      481 cgcgtaaacg cgtctgtaat tctgtcaaac atgaagcgaa ctcagcgaaa aaagtattca
      541 tgcctgccgt aacagattca tcgacctgct ctgccagaac tttagccatt tgttggcaat
      601 agagatcgac ttctgcgctt aatgctcgtt gcaacacact gtaatcaacc gtttctgtcg
      661 gggatttctc atttccccgt ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac
      721 gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc ctggcgcgaa atgacttcac
      781 tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg aaaaacagga aaatgaaacc
      841 caggacgaaa ccctgactcg ctcaattcca ttttgacttg ttgctcaatg gggcgaataa
      901 catcggttaa cacccggcaa agggtggatt ccatctcggc aaaacgcagc gtaaagtcgc
      961 gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca gcgggtacga atctcgctta
     1021 acggctccga atcatcctga aacaaggcgg ctaactgcgc attcaccgca tcttgctgtt
     1081 gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat ttcatgttta atctcgccgc
     1141 tcacctgcgc ctgattgagt tgcaatagct gcaaactttc ttcgacctga tggatatttt
     1201 gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg aaaatccagg tattcccgcg
     1261 cctgctgcgc gtaattcaac agtttatgcg cagcagatcg cagagcatac aatgaggcgt
     1321 tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc gaacagcgaa tcttcccaca
     1381 actgatcggc agcatgacga atatgttcga ggtccgccag atcggcatga cgccagcgcc
     1441 tgccgagcgc ggcatgggca aaatcttcca cccagcgttg ttgctctggc gctggtaact
     1501 taccgttgtt ggctaactca tagcgcgccc gattcgccag gtagccccac atcgacgaca
     1561 ccgggaatat ctgctgtggc gtaatacagc ctttcatcag cgtcccggaa atcagtgccc
     1621 gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc gaacttattg accagcacat
     1681 acagcggcac cgattgcccc accgccaaaa tcgcctcacg gacctcttca tcggagatcg
     1741 atttcagttg cgtataatcc agcaccgcca gtaccgccga ggcgcgtgcc agctgctggt
     1801 taagcatttt ttgcagatgc ggttgcccgg cttcatttgg cccaggggta tccagtaacg
     1861 tcaactgacc gggataactt tccagccccg ccagatggac aaactccact tcaatcacgg
     1921 gaatatgctc aatggcggcg taagcagaaa aaggaaaatc gacgtccagc gccttcgcca
     1981 gtcgcactaa atcattcaaa cttttcagac aatgaaaaat aggctgggca cccagataat
     2041 atttttcgaa agcgacgcca ttttcgatcc gctgcataag cgcacgcata tctttatcta
     2101 tttccagcac atcggtcaga tgcttaatat cgcaatcacg caggcgctgt tgtaattgtt
     2161 gaattaaaca atcgattggc gcgacatgtg aaaaatgcag taccggttcc ttttgcccgg
     2221 gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg attacgatta ggcagaacct
     2281 ccgtaccaac aatggcatta atggtggttg atttccctgc tttcatggta ccgacaattg
     2341 ccagcaccat ttccagtcgg gaaattttac acaactcatt attcagcatc gcgtgacgtt
     2401 cggcgatatt aggctgactc cagggtaaag ccagttgtgg cgcgtcgtct ccgggtacag
     2461 agagaggcat tttttccagt aactgcaact gttggcgaga aagctgtaac aggcgttcag
     2521 cctcctgact taactcatac agggtctgtg tgtacataga aaattcttcc ttaaagcaaa
     2581 ttttgttact tttatttagc cagattgttt ttgagttctg ttttcggctt ttataattac
     2641 tgcaagaaat aattttatat ttagtgtgtt gttttttatc agaataaata acgtcttctg
     2701 atgcgtttaa aacgtcagaa agataaaaat atcatgtgaa taaaaaaaag aacaagtaga
     2761 gcattaacat tatcttaaat aataaataga ggcaaaaaga ttattttctt tttacgtttc
     2821 ctttcaaatg aaaacgatcg tcgtctaaaa tcagcagtac ccccgacaaa ctcagggatt
     2881 ttgtgtataa ttgcggcctt tttcggcaat ctgccgtttt ttggcgcttt tgccctgctg
     2941 acttttgagg aaatccacat gtcattacca cactgcccaa aatgcaactc cgaatacact
     3001 tacgaagata acggcatgta catctgcccg gaatgtgcct acgaatggaa cgacgcagaa
     3061 cctgcacagg aaagcgacga gctgatcgtt aaagatgcta acggcaatct gctggctgac
     3121 ggcgacagcg ttaccatcat taaagatctg aaggtgaaag gtagctcttc gatgctgaaa
     3181 attggcacca aagtgaaaaa catccgcctg gttgaaggcg accataacat cgattgcaaa
     3241 atcgacggtt ttggtccgat gaaactgaaa tctgagtttg tgaaaaagaa ctgattgtat
     3301 tgtgatcggt aagccggata aggcgctcgc gccgcatccg gcaacgatgc cgactgcctg
     3361 atgcgacgct tgcgcgtctt atcaggcagc ctctcatctg tataaatttc gaactacact
     3421 taactggctt ctcttaactg aggtcaccat catgccgtta agtccctacc tctcttttgc
     3481 cggtaactgt tccgacgcga ttgcctatta tcaacgtacg ttgggcgcgg aactgctcta
     3541 taaaatcagc ttcggcgaaa tgccgaaatc agcgcaggac agcgccgaga actgcccttc
     3601 cggaatgcaa tttcccgata ccgccatcgc tcatgccaac gtgcgcattg ccggaagcga
     3661 catcatgatg agcgatgcta taccgtcagg aaaagccagc tactccggct ttacgctggt
     3721 gctcgattcg caacaggtcg aagaaggaaa acgctggttt gataaccttg ccgctaacgg
     3781 aaaaatcgaa atggcctggc aggaaacttt ctgggcgcat ggctttggca aagtcaccga
     3841 taaatttggc gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataacccgc
     3901 cgggaggccc gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca
     3961 cttttaatta accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg
     4021 ctgcatcatg caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca
     4081 agcgctgcat gcggttgatc tgaacattca tcacagtgaa atggtggctc tgcttgggcc
     4141 gtcgggttcc ggcaaatcca cccttttacg tcatttaagc ggtttgatta ccggcgataa
     4201 atccgccggt agccatatcg agctactggg ccgcactgtc cagcgcgaag gccgcctggc
     4261 ccgcgatatc cgcaaaagcc gcgccaacac cggctacatc tttcaacaat tcaacctggt
     4321 gaaccgcctg agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg
     4381 gcgcacctgt tttagctggt tcaccggcga gcagaaacag cgcgcgttac aggcgctgac
     4441 ccgcgttggc atggtgcatt ttgcccatca gcgcgtttcc accctctccg gcggccagca
     4501 gcaacgtgtg gcgattgccc gtgcgctgat gcagcaggcg aaagtgattc tggccgatga
     4561 acccatcgcc tcgctggacc cggaatcagc gcgcatcgtg atggacaccc tgcgcgacat
     4621 caaccagaac gacggcatca ccgtggtcgt cacgctgcat caggtggatt acgccctgcg
     4681 ctactgcgaa cgcatcgtcg ccctgcgtca ggggcacgtc ttctacgacg gcagcagcca
     4741 actgtttgat aacgaacgtt ttgaccatct ctaccgcagc attaaccgca tcgaagagaa
     4801 cgcgaaagct gcctgatatc cccatcattg aggaaaacga atgaacgcta agataattgc
     4861 ctcgctggcc ttcaccagca tgttcagcct cagcaccctg ttaaccccgg cacacgccga
     4921 agagcaggaa aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa
     4981 accgcaatgg acgccgtttt tacaggatat ggagaagaaa ctgggcgtga aggtcaacgc
     5041 cttctttgcc ccggactacg cgggcattat ccaggggatg cgcttcaata aagtggatat
     5101 tgcctggtac ggcaacctgt cggcgatgga agcggtggat cgcgccaatg gccaggtctt
     5161 cgcccagacg gtcgcggcgg atggatcgcc gggttactgg agcgtgttga tcgtcaacaa
     5221 agacagcccg atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg
     5281 caatggcgat cctaactcca cctctggctt cctcgtcccc ggttactacg tcttcgctaa
     5341 aaacaatatc tccgccagcg acttcaagcg caccgtcaac gccgggcatg aaaccaacgc
     5401 gctggccgtc gccaacaagc aagtggatgt tgccaccaac aacaccgaaa acctcgacaa
     5461 gctgaaaacc tccgcgccgg agaagcttaa agagctgaag gtgatctgga aatcgccgct
     5521 gatcctaggc gatccgatcg tctggcgcaa gaatctgtcc gaaactacca aagacaagat
     5581 ctacgacttc tttatgaact acggaaaaac gccggaagag aaagcggtgc tggaacgcct
     5641 gggctgggcg ccgttccgcg cttccagcga cctgcaactg gtgccgattc gccagctcgc
     5701 actgtttaaa gagatacaaa gcgtgaaagg caataaaggg ctgaatgagc aggacaagct
     5761 ggcgaaaacc accgagattc aggcgcagct ggatgacctg gaccgcctga acaacgcgtt
     5821 aagcgcgatg agttcggtga gtaaagcggt gcagtaaatc gtaggtcgga taagacgccc
     5881 cggcgtcgca tccgacaatg tgtaggcgtt gatgccggat gcggtgcaag caccttatcc
     5941 ggcctacaga ccggagccaa acatgcaaac catcaccatc gccccaccca agcgtagctg
     6001 gttctcgctt ctgagctggg ccgttgttct cgacgtgctg gtcgtctcgt ggcagggcgc
     6061 ggaaatggcc ccgcttacgc tgattaaaga cggcggcaac atggcaacct t
//
LOCUS       DADLCQ010000185         6052 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000185 DADLCQ010000000
VERSION     DADLCQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.187"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            430..1338
                     /locus_tag="JQG95_003777"
     CDS             430..1338
                     /locus_tag="JQG95_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY5890177.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKVEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQG95_003778"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="JQG95_003778"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY5890178.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /locus_tag="JQG95_003779"
     CDS             3653..4411
                     /locus_tag="JQG95_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY5890179.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQG95_003780"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="JQG95_003780"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY5890180.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="JQG95_003781"
     CDS             complement(5673..>6052)
                     /locus_tag="JQG95_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890181.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1575 c         1561 g         1425 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagtaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggaa atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgtcaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 ccgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DADLCQ010000186         6028 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000186 DADLCQ010000000
VERSION     DADLCQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.188"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="JQG95_003782"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="JQG95_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY5890182.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="JQG95_003783"
     CDS             1207..1869
                     /locus_tag="JQG95_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5890183.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="JQG95_003784"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="JQG95_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY5890184.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="JQG95_003785"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="JQG95_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="JQG95_003786"
     CDS             2795..3100
                     /locus_tag="JQG95_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY5890185.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="JQG95_003787"
     CDS             3103..3507
                     /locus_tag="JQG95_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890186.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="JQG95_003788"
     CDS             3497..3796
                     /locus_tag="JQG95_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890187.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3892..4278
                     /locus_tag="JQG95_003789"
     CDS             3892..4278
                     /locus_tag="JQG95_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY5890188.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLLTAFLFHSNFAEGVNSLMFMKNLT
                     ISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4348..5334
                     /locus_tag="JQG95_003790"
     CDS             4348..5334
                     /locus_tag="JQG95_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY5890189.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="JQG95_003791"
     CDS             5627..5995
                     /locus_tag="JQG95_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY5890190.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
BASE COUNT         1524 a         1470 c         1519 g         1515 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaatt
//
LOCUS       DADLCQ010000187         5983 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000187 DADLCQ010000000
VERSION     DADLCQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.189"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..393)
                     /locus_tag="JQG95_003792"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="JQG95_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JQG95_003793"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="JQG95_003793"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY5890191.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGIIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="JQG95_003794"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="JQG95_003794"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY5890192.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="JQG95_003795"
     CDS             5797..>5983
                     /locus_tag="JQG95_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890193.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1839 a         1354 c         1019 g         1771 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattatac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DADLCQ010000188         5983 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000188 DADLCQ010000000
VERSION     DADLCQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.190"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..262)
                     /locus_tag="JQG95_003796"
     CDS             complement(<1..262)
                     /locus_tag="JQG95_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY5890194.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFV"
     gene            483..911
                     /gene="uspG"
                     /locus_tag="JQG95_003797"
     CDS             483..911
                     /gene="uspG"
                     /locus_tag="JQG95_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY5890195.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="JQG95_003798"
     CDS             complement(1032..2597)
                     /gene="ahpF"
                     /locus_tag="JQG95_003798"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY5890196.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="JQG95_003799"
     CDS             complement(2842..3405)
                     /gene="ahpC"
                     /locus_tag="JQG95_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY5890197.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3777..4523
                     /gene="dsbG"
                     /locus_tag="JQG95_003800"
     CDS             3777..4523
                     /gene="dsbG"
                     /locus_tag="JQG95_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY5890198.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4733..5635
                     /locus_tag="JQG95_003801"
     CDS             4733..5635
                     /locus_tag="JQG95_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5890199.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            5782..5983
                     /locus_tag="JQG95_003802"
                     /pseudo
     CDS             5782..5983
                     /locus_tag="JQG95_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         1605 a         1441 c         1348 g         1589 t
ORIGIN      
        1 tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc ttcccggttt
       61 caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt
      121 tgcctcgata aagatggagg tcagagccac agatcgccgt taccgtaata cgcagaataa
      181 tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc
      241 cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt
      301 aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctagt
      361 gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata
      421 tcagggataa atattataat catggagaat gatttgttca tgattaacag ggagaaaggg
      481 ttatgtataa ggcaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag
      541 ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac
      601 tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag
      661 agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg
      721 atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt
      781 tggcagaaga actgggggct gatgttgtag ttatcggttc tcgcaaccca tcgatttcga
      841 cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg cctgtactgg
      901 tcgttcgcta acagataaaa gtatttatgc cgccctgcct ggaacatccc ggcagggctt
      961 tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat
     1021 cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct
     1081 ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt acagtcaccc
     1141 gctgcgaaca cgcctttgac gttggtttca catttcgcat caatgataat ctcgcccatg
     1201 cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggaca
     1261 aaaataccgg ccagttcgat gttgtgaata ttgccgctga cacgatctcg atattccaga
     1321 ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg
     1381 acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca
     1441 aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg
     1501 gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggtcc gtcgcagtgc
     1561 gggcagtagg tcacgccttt agtgcgatac tgatcttcgc ccggaacgtt catgttgcgc
     1621 cattttgcac ccgtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt
     1681 tcaatctggt gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg
     1741 tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc caacttctgc
     1801 ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca
     1861 ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc
     1921 gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg cttgttcagc
     1981 tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc
     2041 atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg
     2101 cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg
     2161 ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc
     2221 gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac
     2281 tgcgcttctt tcgacggatg accaccggtc cacagcaacg ccagtaccag cgaggtgaac
     2341 tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt tggaacctgg gttggtgatc
     2401 aggaaagacg gcttacgcac tggcaagctg ttatcttctt taaaggtgac tttgtctgac
     2461 agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccagc
     2521 gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc
     2581 atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct
     2641 gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac
     2701 ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga gcaaggcgac tagcctgtga
     2761 atccccggga gcttacacaa gtaagtgacc ggggtgagcg ggcgacgcca acgccgctat
     2821 ggcgtgaaag acgaaggaaa tttagatttt accaaccagg tccagagacg gagccagagt
     2881 tgcttcacct tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg
     2941 agctgctttg attttacgca gcagatcaga cgcgtcacgg ccaatgcctt cagcggtaac
     3001 ttcgattgcc tggatgatac cctgcgggtc aacaacgaag gtcgcacggt cagccagacc
     3061 ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat
     3121 cgcatatttg attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg
     3181 agtatcggta gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc
     3241 agcaacgtca cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa
     3301 gacgctccag cggccttcgg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa
     3361 tgcctggttt ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt
     3421 gttttcgatg agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat
     3481 caaatcgata agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc
     3541 agcggcgata aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct
     3601 ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc cttacaaatg caacaacgac
     3661 atggattaca acaccctcat aaacaaaggg caatcacctg atctaagctc ttacctatga
     3721 cagtgatagg ttatgccttt tactcgactt ttgcactgac tgaaaagaac aaattaatgt
     3781 taaaaaagat acttttactg gctctgcttc ctgcaatcgc cttcgcagag gaacttcctt
     3841 ccccagtaaa agcgattgaa aaacagggca ttacaatcat caaaacgttc gatgcccccg
     3901 gaggaatgaa aggttatctc ggaaagtatc aggatatggg cgtcaccatc tacctgactc
     3961 ccgatggtaa gcacgctatc tctggttaca tgtacaacga gaaaggtgaa aacctgagta
     4021 acacacttat cgaaaaagaa atttacgcac cagccggacg cgaaatgtgg caacggatgg
     4081 aacaatccca ctggctcctc gacggtaaaa aagatgcgcc ggtcattgtc tacgtcttct
     4141 ccgatccgtt ctgcccatat tgtaaacagt tctggcagca ggcgcgcccg tgggtagatt
     4201 ctggcaaagt gcaattaaga acattgttgg ttggggttat caagccagaa agcccggcga
     4261 cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac ctggcaacaa tatgaagcct
     4321 ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag tacagagcaa atgaaagtgt
     4381 taagtgacaa tgagaaactg atggacgatc tgggggcaaa tgtcacgccg gctatctatt
     4441 acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt gcccgatcag aaaacgctta
     4501 atatcattat gggaaataaa taaaaggtaa ttcatggatg gttgaactat atccctgctt
     4561 ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc taagtggctg tgctatttta
     4621 gaaaaactta atcctgagtt aagcaaaaaa acataatcaa taaaaataca tacgcaaaaa
     4681 acattgacta agtgaatata ccatggaaga aaaatataaa ccggagtagt gtatggccaa
     4741 tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc atatttgagt gtatttacca
     4801 gcatttaagc attagcaaag ctgctgaatc actatatatc accccatcag ccgtgagcca
     4861 gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta ttcattcgct caggaaaagg
     4921 tatcgcgccg acgacaacag ggttgaatct gcatcaccat cttgagaaaa acctcaaagg
     4981 ccttgaacaa acaattaata tagtcaataa gtccgaacta aagaaaaact ttattatcta
     5041 cggccctcag ctcatttctt gctctaataa tagtatgctg atccgctgcc tacggcaaga
     5101 tgcctctgtc gagattgagt gccatgatat cctcatgtca gcagagaatg ctgaagaact
     5161 gttggttcac cgcaaagccg atctggtgat cacccaaatg ccagtgatca gccgttccgt
     5221 catttgcatg ccattgcata caattcgcaa tactctaatt tgtagtaata agcatccgcg
     5281 aattactgac aatagcactt atgaacaaat tatggcagaa gagttcaccc aattaatagc
     5341 caaatcagca ggagttgatg atattcaaat ggaaatagac gagaagttta tgaatcgcaa
     5401 aatttcattt cgtggttcct ctttgctgac aataattaat agcattgcag ttacagattt
     5461 actgggaata gtcccctatg aactttataa ctaccaccgt gactttctta atttaaaaga
     5521 gataaaacta gaacatccgt taccttcaat aaagctttac atttcctata ataaatcttc
     5581 tttaaataat ttggtgttct cccgatttat tgaccgtcta aatgagagtt tttgatatat
     5641 aactatagac agctactata actttcatct atttattcac agcgcgaatg aaaaataaaa
     5701 cactctgctt tacagtgttt actttataat taacagtctt atctcactgt tcaccctgta
     5761 acgttcagag agcgtcttca gatgtctatt tataaaattc ctcttccgct caatattctg
     5821 gaagctgcac gagagcgtat tacctagaca ctcaacaccc taccccgcgt ttgtgtctca
     5881 ttttctggcg gcaaagactc tggtttaatg ctgcatctga cagccgaact tgcccgacaa
     5941 atgggcaaaa aaatctgcgt tttgtttatc aactgggagg cgc
//
LOCUS       DADLCQ010000189         5805 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000189 DADLCQ010000000
VERSION     DADLCQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.191"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JQG95_003803"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JQG95_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY5890200.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JQG95_003804"
     CDS             578..1027
                     /locus_tag="JQG95_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY5890201.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1261)
                     /locus_tag="JQG95_003805"
     CDS             complement(1028..1261)
                     /locus_tag="JQG95_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY5890202.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1347..1520)
                     /locus_tag="JQG95_003806"
     CDS             complement(1347..1520)
                     /locus_tag="JQG95_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY5890203.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1715..1810
                     /gene="yncL"
                     /locus_tag="JQG95_003807"
     CDS             1715..1810
                     /gene="yncL"
                     /locus_tag="JQG95_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY5890204.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2212..3021)
                     /locus_tag="JQG95_003808"
     CDS             complement(2212..3021)
                     /locus_tag="JQG95_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY5890205.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQG95_003809"
     CDS             complement(3231..4655)
                     /gene="patD"
                     /locus_tag="JQG95_003809"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAY5890206.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4677..5471)
                     /locus_tag="JQG95_003810"
     CDS             complement(4677..5471)
                     /locus_tag="JQG95_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY5890207.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..>5805)
                     /locus_tag="JQG95_003811"
     CDS             complement(5461..>5805)
                     /locus_tag="JQG95_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5890208.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1523 a         1473 c         1286 g         1523 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt atatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagtcg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcattggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg cgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcaac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggcgcgct tccatatctt
     1081 ctttatgctt gcgaccgtga tgagcaatag cggtgcgcaa acgctgctgc tgggtgtagc
     1141 gatcttcccg acttaactcc gcgtcatcgc tcagggcgat taataattcg ttcatatggg
     1201 caatgacgct ctcttcaatc gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca
     1261 tactttctcc ttagccactt gtgaaattta tcgattaatc caaatgatat aagtctgcgt
     1321 ggtgccgtca agccagtgag taagatttaa aacagtgaaa acaacagcgc caccgggaaa
     1381 ctcatcggcc atgtggcccc caccagaaag gcgcttaaga aacggatgcg tttcttatcg
     1441 tgagaaagaa accaggtgat aagaaatgcg attgctgcca taaccgcata aaaaacaagc
     1501 atatgttgat agagagacat gtacgggcgt ccagccatat gaataaaacg cgcgcaaata
     1561 tgcgccattt tttgacgtgc atcaagtttt ttcaccagtt tgagaaaaaa tcatcaaaca
     1621 gcaacttgcc cttatccatc ttcagaatga gtattattgc ggattttctt aacccgtact
     1681 atacacatag gggcaatgat aaaaggtggc aaaaatgaat gtttccagta gaactgtagt
     1741 actgataaat gtctttgctg ctgttggttt gtttactctt atctctatga gatttggctg
     1801 gtttatttga tgtagtgatt ttgcacgcag cacgtactgt cagagaaatc aactctctga
     1861 cagtacgcaa gatcttctca tactctccaa gtttgcaaca ttatccttaa tatttacttc
     1921 tttttgctgg catctacatt gaaatgagta gaaaacatcc ccctactgtc gtctatacaa
     1981 acagcttact gattcaaacg gaatcttgtt ttcacatttt tctatcacga atactttata
     2041 cgcgcgggag tctataaatg ctttatactt tcaaggaata acgtaaatat aaaaagatgg
     2101 cgtaaagtat atgcatggca ggatgtttgc aacctccttg atgacgctgc cccattgaaa
     2161 ttgcaaaccc cgccctggtt atcttgccca tttatcatgc aaaacagata attataggcc
     2221 tctgaatacc tcagaaagat tgaatttttt atagtccctc tcttgaatat ttccatttga
     2281 tatcaatcga ttcatctctt caacaaaata atttatatca tcacacttat tattatccgg
     2341 aagatctgag tagttataat tccaccattt tattctcaac agttcatcaa taacatttga
     2401 atcaaaccta aatctgatta tttttgcagg aactcctgca actatggcat atgggggcac
     2461 atctttggta acaacggaat tggcagcaat aacagcacca tcacctattg cgatgccacc
     2521 tttcaataca acatcatttc ctatccagac atcatttcct ataatcggat tttcaggttt
     2581 ggtaatgaat ggtttcaatg tccagctacg ttcaaattca ctcatagcaa acttatcaaa
     2641 ttcaccgtta taagtcatca tgtgagtagt aaaacgattc aaaggatgat ttccacccat
     2701 tatttttaca tttgatgcaa tactacaaaa tcgacctata cgtatattcc ctgaaaaatg
     2761 gcaggtcgga aatgaaaaag cgccgacact ggagaaattt ttcacaggca ttgttgaata
     2821 ttcttcagca ataacattat cttcaattgt tatcttgtca ccttttgaga aaagaaataa
     2881 attcctttct ttatacgact tcttcccctt atgatttaaa aagattttat tatccataca
     2941 aaactcaaag tgttttttcg tccatctgaa ggtaagggtt ttatttttat cttccgtgaa
     3001 tttgtacaat tgaactttca ttgttaatac ttctacttat aaagtattag tttaataacg
     3061 atactctaaa gccttatgct aatcaactgt gacaggatga aaaatcgcca tcaaacattt
     3121 agcctaattt ttacgccagt tattaatgta atcattctaa tacttaaccg ccattaaact
     3181 cagaacctaa aacgatagtg gccgtcctat aacagccact aacatctaac ttaatgttta
     3241 accatgacgt ggcggacgac ggtgtaatcc tccagcccat aaagtgacat atccttgccg
     3301 taaccagaaa gtttctgccc accgtgcggc atttcactta ccagcatgaa atgggtattg
     3361 acccaggtac aaccatattg cagccgtgcg ctgacgcgat gcgccctgcc cacatctttc
     3421 gtccataccg aagatgcaag tccgtactgg ctgtcattcg cccagttcac cacctgttct
     3481 tcgttgtcga agggcgtaac actcactact ggaccaaata cctctttttg cacgatggca
     3541 tcgtcctgta atgcgccagc cagcagcgtc ggcgcatagt aatagccatt acccttgcgc
     3601 ttttcaccgc cagtgatcac tttgatgtgc cctgtcgctt tcgcctcttc tactgccttg
     3661 ctgacgcgtt cgagatgcgc cagcgagctt aaaggtccaa gctccgtaga ctcgtcatcc
     3721 ggcgcaccag attttaacgt tgccaccgca gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaagata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatac ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 acccgttctc cgttaatcag taacttatgt tgcatagctt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgct aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggat
//
LOCUS       DADLCQ010000190         5784 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000190 DADLCQ010000000
VERSION     DADLCQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.192"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..941)
                     /locus_tag="JQG95_003812"
     CDS             complement(<1..941)
                     /locus_tag="JQG95_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAY5890209.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLN"
     gene            complement(941..1612)
                     /gene="hprR"
                     /locus_tag="JQG95_003813"
     CDS             complement(941..1612)
                     /gene="hprR"
                     /locus_tag="JQG95_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY5890210.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1745..2158
                     /gene="hiuH"
                     /locus_tag="JQG95_003814"
     CDS             1745..2158
                     /gene="hiuH"
                     /locus_tag="JQG95_003814"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY5890211.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2267..3271
                     /gene="msrP"
                     /locus_tag="JQG95_003815"
                     /pseudo
     CDS             2267..3271
                     /gene="msrP"
                     /locus_tag="JQG95_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            3272..3907
                     /gene="msrQ"
                     /locus_tag="JQG95_003816"
     CDS             3272..3907
                     /gene="msrQ"
                     /locus_tag="JQG95_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY5890212.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4165..4815
                     /gene="zinT"
                     /locus_tag="JQG95_003817"
     CDS             4165..4815
                     /gene="zinT"
                     /locus_tag="JQG95_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY5890213.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1643 a         1305 c         1196 g         1640 t
ORIGIN      
        1 ttattgagag aaagcgaatc cagtttcacc aaaacattgt ttttgtctgc cctggcaaga
       61 aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt tccggcaatt
      121 gttttttgat attcagcgat acttctgatt tgactgagcg taacctgatt ctgacccagt
      181 aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg actaaggcgc
      241 tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt
      301 tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt cagcgcttcc
      361 gttacgccac tcaacttttt gatctctcgc aaccccgtcc tgattaacaa cggacttaat
      421 actgagcaca gtataatggc gacaatgcaa attataatgc tattaatttt atactgttca
      481 agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact
      541 tcatcaatgt taattcgtaa agcatctatc tctgtatcat taataatgct tctgtaaatg
      601 cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt
      661 gtccggttaa caattttatt gatgccatca ctatgaatga tcaaaatatc ctgactaaca
      721 tccatcatcc ggttaaagta cacaggtaac gtatctggat ttaccccatc aattaacaac
      781 tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt
      841 gccaggccat tatagagagt ccatacaatt ccagcgccag caacagactg cagaaatata
      901 aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga
      961 atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt
     1021 ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc
     1081 cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg gaggccagta
     1141 gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctgacac
     1201 tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc agagcgtgat
     1261 gttgccttaa ttgtgcccga acccttgcta gcaactcaga aaatgaaaaa ggttttacca
     1321 gataatcatt tgccccactg tccaaccctc tgactctgtc atcgacagaa tcccttgcag
     1381 taaggcaaat aacaggggtt tgctttgccg ttcttaacgt ttgtaagatc tgccagccat
     1441 ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat
     1501 aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct
     1561 gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc atgaaaataa
     1621 tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg taatgctgct
     1681 gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg acaaggatat
     1741 aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt cattaccttc
     1801 tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaacagg
     1861 aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg gctggttaca
     1921 acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac
     1981 tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa
     2041 tcttgaaagt ttcttccctg agattcccgt tgaatttcat attaataaag tgaacgagca
     2101 ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg gcagttaatg
     2161 cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct gacagattaa
     2221 cagtaaacag ctcttgttgg ttaacgacaa aaaagtgtga tggcttatga aaaagaagca
     2281 atttttaaaa gaaccagatg ttacggccga gtcggtattc tttatgaagc gtcggcaggt
     2341 gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg cgcatgccga
     2401 tctgcttagc tggtttaaag ggaacgatcg tccgcccgcc cccgccggaa aaccgctgga
     2461 gttcagcaag cctgccgcct agcaaaataa cctgccactg acgccagcag ataaagtctc
     2521 cggttataac aacttctatg aattcgggct ggataaagac gatcccgcgg ctaatgctgg
     2581 tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa aaccattgac
     2641 cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg
     2701 ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac acaaattact
     2761 ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga tttatgcgcc
     2821 ggaacagatg cctggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt
     2881 cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg taggtgttta
     2941 tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc cgtggaaata
     3001 tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc gtccgccaac
     3061 cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt
     3121 tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg gcattctcga
     3181 tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg catcgctgta
     3241 tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac aagtgatatg
     3301 gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat
     3361 caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac
     3421 tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct acgcaaaaca
     3481 gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct gggcgacact
     3541 gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt tattaggtaa
     3601 ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc
     3661 tttagcgttc acttcaaccc agtcgatgca gcgaaaactc ggcaaacact ggcaacagtt
     3721 gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa
     3781 gattatttca ccgcagccac tcatctacgc tgggctggct gtactgcttt tagccttacg
     3841 gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca acaaattatc
     3901 tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg gcgcgagcgc
     3961 cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa cctttcatca
     4021 ccagaataat ttattttatt tcaataagat agttcaatac acctgagaat accatgctct
     4081 catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta cacataatgt
     4141 acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc tgttgcttta
     4201 ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcattctca tggcaaaccc
     4261 ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa tgtacaaaac
     4321 cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact gcaaagtggc
     4381 aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac atttgctgaa
     4441 attaaagatt attatcgcaa aggttatccg acagatatcg aaatgattgg cattgaggac
     4501 ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga ttacgatgga
     4561 tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt cgaatgtaaa
     4621 gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat tgcaccacga
     4681 aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt attgaatgaa
     4741 atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt ggtcgaggag
     4801 atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca gtgcctggta
     4861 gtaacaacaa taatttacca agacaaataa gtaagacaaa ctcagacacg ctcatacttg
     4921 agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcta acaataaata
     4981 cacaactcag ccatacagac tggcagcgca aatgtttcca ggagcaagtc atctgtcatg
     5041 gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta cttgttagta
     5101 tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat tatggacttt
     5161 gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt gaccccttct
     5221 ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc aatgtctaaa
     5281 agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc tcgaaacctt
     5341 cctgtcagct ccatagcgaa tcaagcgctg aatgtcacag tatcgaacag aaaacagtga
     5401 cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca aggcgggaag
     5461 tcgggagata agtcattgat aaaatggcgg agagaggggg atttgaaccc ccggtagagt
     5521 tgcccctact ccggttttcg agaccgatga tacagagtcg aaaaatcaaa gagttaaaaa
     5581 tttttcttgg aaaaaatgtc ttaaaatcgt acacgaaaaa tcaatgaatt acataacatt
     5641 aaagaagaat attccaagcc ttcttgagtc caccacctgt tttcaggtgt cattcagacg
     5701 tgatttgccc cagattggga aagcacgctt ttttctcagc ttattccgtt ttgcctgtgt
     5761 gttgaataat tccactgcag ttat
//
LOCUS       DADLCQ010000191         5759 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000191 DADLCQ010000000
VERSION     DADLCQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.193"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..402)
                     /locus_tag="JQG95_003818"
     CDS             complement(<1..402)
                     /locus_tag="JQG95_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890214.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTAL"
     gene            complement(547..1050)
                     /gene="cheW"
                     /locus_tag="JQG95_003819"
     CDS             complement(547..1050)
                     /gene="cheW"
                     /locus_tag="JQG95_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY5890215.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="JQG95_003820"
     CDS             complement(1071..3035)
                     /gene="cheA"
                     /locus_tag="JQG95_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY5890216.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3040..3966)
                     /gene="motB"
                     /locus_tag="JQG95_003821"
     CDS             complement(3040..3966)
                     /gene="motB"
                     /locus_tag="JQG95_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY5890217.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3963..4850)
                     /gene="motA"
                     /locus_tag="JQG95_003822"
     CDS             complement(3963..4850)
                     /gene="motA"
                     /locus_tag="JQG95_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY5890218.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGVLIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="JQG95_003823"
     CDS             complement(4977..5555)
                     /gene="flhC"
                     /locus_tag="JQG95_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY5890219.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRARTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5558..>5759)
                     /locus_tag="JQG95_003824"
     CDS             complement(5558..>5759)
                     /locus_tag="JQG95_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY5890220.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         1342 a         1553 c         1407 g         1457 t
ORIGIN      
        1 taacgctgtg taatagtttt tatatttttc atcaaaatta cggctggtag cgaccatttc
       61 aggtaacggt gccatgcttt tgaatttttt ataatgcgtc gcggcctggg ccaatgtttt
      121 cctagcgcta tcgagcaatt caactttggc gttactttgt tgattggagg aatccatcat
      181 catccgcacc gctgaacgac tcaggttaat gcgcgtttgc agcattaaat cccaggttga
      241 cgtcagctcg ccctgctgtt cccgtaattg attggaaacc acaaagctct tctggctatg
      301 gtgaagggaa gaaaaaaaca aactgccgga aataagctgt aacagtgcga atacccccag
      361 caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc ttcctgaaaa
      421 caagttgatc tcgttatcgg caagaagggg ggaaacttta ttgctgatgc cacccgccgc
      481 gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt gaggaaatcg
      541 ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt cgctgttcag
      601 cagtttttcg atgttcacca gaatcaacat acggtcgccc agtgcgccca gtccagtgag
      661 atattctgtt gaaagcgtca cggcaaactc tggtgccgga cgaatttgct ccgccgtcaa
      721 tgaaagcacg tctgaaacgc cgtcaaccac gatgccgacc acccgctgtc cgagattcag
      781 gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact taattcgtaa
      841 gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa acgctggcgt
      901 gttcgcaatc cgtgttacct gatcgtagcc acggatctcc tgcactttca ggatatcaat
      961 accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg gctcgctggc
     1021 cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat tcaggcggcg
     1081 gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc aacaatcagt
     1141 gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt gcgatagtta
     1201 ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac cagcaaggcg
     1261 tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc ggttttcgcg
     1321 cccgcgacgt tgaacacttt ccacagttcg acgatgggca gatattcacc ccgcacttcc
     1381 agcacccgct cgccgccggc cagtggatgg agatcggctt cacggggttg cagtgattcc
     1441 ataacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga catgccgtcg
     1501 aggatggcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc ctgcttcgac
     1561 tggatttcga catgaccgcc catctcctgg atattacgtt taacgacgtc catgccgacg
     1621 ccgcgcccgg agacgtcggt gacctgctct gccgtggaga agcctggtgc aaatatcagc
     1681 attgcaactt cgtcgtcgct catgttttcg ctgacagtca aaccttgcga ggccgctttt
     1741 gccagaattc gctcacggtt tagccccgcc ccatcgtcgg tcacttcaat gcaaatgttg
     1801 ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc tgcggcgagc
     1861 cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg ggtcagcggg
     1921 tcgataatgc gttctatcag gctcttgtcg agctcggtgg aactgcccac cagcgtcagt
     1981 tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata gcgactaaag
     2041 acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc acgggcgtta
     2101 cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc cagttcgctg
     2161 gaacgctggg caagcatgga ctgggtgata accagctcgc cgacgaggtt aattaattga
     2221 tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt cgttttttcc
     2281 cgctccacgc ggccggttgg cgcttgttcg gctgccagtt taagcacagg tggggtagct
     2341 atttttggcg aaacttctac tgtttcaaag gtaatctgat cggcttcaat cacaaaacag
     2401 agtaccgctg tgatgtcatc ttcggcgata tcgcccggta atattgccga gagagaatcc
     2461 gcccctttca ccacgtcatt taacgttgtc agatgcccca gctcttcttc cagcaggtcg
     2521 acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gcaactgact gcgactttgc
     2581 tcatcttgcg gttcactttt ggcaaccaca cttaatcggg tcactgcgga tggcgtttcg
     2641 cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgta gctggcggca
     2701 tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc cttcgtttcc
     2761 aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc ttcatcgagc
     2821 aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaagt tcctgcccct
     2881 cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc tggcgcttcc
     2941 ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc ttcatcaaaa
     3001 aatgtctgat aaaaatcgct tatatcgatg ctcacgctgt cacctcggtt cggctgatgg
     3061 cattgtggga acactgacct gtggtgcaac ctcaggtttt tctagggcgc ttactggctc
     3121 attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt tcagtaccaa
     3181 caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc gcatcgttgc
     3241 cgccatgccg acgacacgta acactttgcc gctatccaac cctccgacca tcagttcgcg
     3301 gcgggatgca ttggcccgat cggcagaaag ctcccagttg ctgtaacctt tctcaccgct
     3361 ggcgtaggga aaatcatcgg tatgacctga aaggctaata cggttgggaa taccgttcag
     3421 cacaggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacatcgg cactgccggt
     3481 tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt cctggaccag
     3541 atcgattttg agatggagac gtaacgcccg cagtttcgga tcggactcta tcaactggtc
     3601 gagatcaccc cgcaatttcc gcaatcgact ttgctccatg cgttttttca gctcttcgat
     3661 gttcggctgc ttattcactt ccccctggct ctgggtgtaa tcatcaccac cgccgggaat
     3721 tgggctttca ctattggaaa tgcgatcgcc gcccgtaacc gctgtcgcca gtggagtccg
     3781 gaagtactcc gcaatctgaa tcaactcttt tgggctggag atggagatca gccacatcac
     3841 caaaaaaaag gccatcatcg cagtcataaa gtcggcataa gcaatcttcc acgatccatg
     3901 tgctgccccg tggcttttgg ctttgcgtcg tttgacgaca ataatcggat gcgcttgatt
     3961 cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg cacatgctct
     4021 tccagttcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc aaactcaacg
     4081 gcgataggcg gtgcgtaacc gttcagatta gaaagcagag tgactttgac gcactgcatc
     4141 attttgctgg tttcggcgct tttctgacgt aaaacactcg ctagtgggga aataaatccg
     4201 taagccaata aaataccgag gaaagtcccc accatcgcat gtgcgataag cacccccagc
     4261 tcggcggcag gacgatcggc tgaacctaac gcgtgaacgc cccccattac agccgcgaca
     4321 ataccaaacg ccggaagtga gtccccgacc agcgccagac tgttcgccgg gacttctgcc
     4381 tcgctttcgt gcgtctcaat ctcttcatcc atcagggctt cgatttcgaa ggtgttcatg
     4441 tgaccgctga taatcaggcg cagataatcg acgataaaat caagcatgac gctatccgcg
     4501 aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat atcacgttcc
     4561 agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag agccagcaga
     4621 tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag cgctttcagc
     4681 gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc tgcaccggca
     4741 ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt catcaaataa
     4801 ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac gacatcatcc
     4861 ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacgcgcg attggggaaa
     4921 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgccacaccg tatcagttaa
     4981 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
     5041 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
     5101 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
     5161 agtaatccac tttcaacaaa ccgcaccaat gtcctggcac gggttaatgc cagcagtggt
     5221 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
     5281 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
     5341 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
     5401 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
     5461 tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc cagctgaata
     5521 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
     5581 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
     5641 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
     5701 caaaacggaa gtgacaaacc agctgattgg tttctgccag cttaaccatt tgcggaaga
//
LOCUS       DADLCQ010000192         5702 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000192 DADLCQ010000000
VERSION     DADLCQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.194"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..219)
                     /locus_tag="JQG95_003825"
     CDS             complement(<1..219)
                     /locus_tag="JQG95_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY5890221.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
     gene            complement(216..1124)
                     /locus_tag="JQG95_003826"
     CDS             complement(216..1124)
                     /locus_tag="JQG95_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY5890222.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAVFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            1290..2087
                     /locus_tag="JQG95_003827"
                     /pseudo
     CDS             1290..2087
                     /locus_tag="JQG95_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="JQG95_003828"
                     /pseudo
     CDS             complement(2138..2790)
                     /gene="pphB"
                     /locus_tag="JQG95_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="JQG95_003829"
     CDS             complement(2896..5457)
                     /gene="mutS"
                     /locus_tag="JQG95_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY5890223.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5533..>5702
                     /locus_tag="JQG95_003830"
     CDS             5533..>5702
                     /locus_tag="JQG95_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890224.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
BASE COUNT         1339 a         1480 c         1470 g         1413 t
ORIGIN      
        1 ctgttgcaac cagctcagag ccgccagcga ttgctgtgtg gcttcaacca ccggacagga
       61 gcgcgttttc aggctgatca ccagtgcgtc gattgcttcc ggcattttac ctgttggaac
      121 accgttaatt tgtaccgttg gtagaccgtt ttccaccaga aaactggcga tatccgtcgc
      181 gccggtaaaa tcatcggcga taacgccaat cttgatcatg atttcgctcc cggtagagtg
      241 atgccagaga aaatcttgat aactgcgcta tcgtcttctt tcccgtaacc cgcgttactg
      301 gcgctggtga acatattcaa tgctgttgag gccaatggca gcgggaagtg cagggctttg
      361 gctgtatcgg caaccagacc aagatcctta acaaaaatat cgacggctga atgcggggtg
      421 taatcgccat ccaccacatg acgcatccgg ttttcgaaca tccaggaatt tccggcggca
      481 ttggtcacga cgtcatacat cacatccagc gggatccccg cacgggctgc aagtgccatc
      541 gcttcggctc cggcagcaat atgtacgccc gctaacaact ggtgaataat ttttacggtc
      601 gaacctagtc ccggttctgc acctatgcga taaacttttc cggcaacggc ttccagcacg
      661 ggtgccagtc gttcaaaggc aatatcgcta ccggaggcca tgacagtcat ttcaccgtta
      721 gcggctttta ctgcaccacc agaaactggc gcatccagca tttccagatc gaatccagcc
      781 agagcggtag caatttcttg cgcatcagca ctagcgatag tggaagaaac cattactgcc
      841 gtaccgggtt tcagatgttg tgcaacgcct gtttcaccaa acagcacctg tttaacctgg
      901 gccgcattga ccaccagcac cagcagagcg tccagttttt cggcaaacgt cgcggcgtta
      961 tcagaaaccc cgcaagcacc tgcctctttc aacgtagcgc aggcattgct gttcaggtct
     1021 gcgccccagg tagaaaaaac tgcgcggaca catgacagtg ctgctcccat tcccattgac
     1081 cctaagccaa cgataccgac atgaaactca gatcccgttt tcatctgctc tccttgttaa
     1141 tttaagtgat attttgtttg atattgtgaa cataagcgct gggagataac gatatggtga
     1201 gatgattcac ataaattaac attttgtgtt attttatgtg aactaagcgt tagttgcgcc
     1261 gcgcacgttt cgcaggcaaa tagcgtagaa tgtcagcagg acaaaggaag gagcaaaagt
     1321 tgatacccgt agagcgtcgc caaatcatcc ttgagatggt agctgaaaaa ggcattgtca
     1381 gtattgctga actaacggac agaattaatg tgtcacatat gaccattcgt cgggatttac
     1441 aaaaactgga gcagcaggga gccgttgtgc tggtgtccgg aggcgtctag tctccgggac
     1501 gcgtggcgca tgaaccttct catcaggtaa aaactgcgct ggcaatgacg caaaaagcgg
     1561 ctattggcaa gctggcggca agtcttgttc tgccgggaag ttgtatctat ctggatgcgg
     1621 gaacgaccac gttagcgata gcacagcatc tgattcacat ggagtcactg actgtggtca
     1681 caaacgattt cgttattgcg aactacttgc tcgacaacag taattgcaca attattcaca
     1741 ctggcggtgc agtgtgtcgg gaaaaccgtt cctgtgtcgg ggaagccgct gcgaccatgc
     1801 tgtgcagcct gatgattgat caggctttta tttctgcatc gtcatggagt gtgcggggga
     1861 tttctacgcc agcggaagat aaagtcacgg tgaaacgggc gattgccagt gccagccgcc
     1921 agcgagtttt ggtctgtgat gcgacgaaat atggtcaggt ggcgacatgg ctggcgttac
     1981 cattaagcga gtttgatcag attattacag acgacggttt gccggagagt gccagtcgcg
     2041 cgctggcgaa gcaggatctc tctttgctgg tagcgaaaaa tgaataatgg cctgcaataa
     2101 catctggtta ctcatgcttc acagaagaag catgagacta ccttatttta taaaatgaca
     2161 gccgcccact tttcggcgat ccggtatcaa tataaatctg gttagcgaac gtctgaatgt
     2221 tatcaaacat catatgtcca aatataaaat aatcagcgcc gtttatttgt tgtaactcgc
     2281 cattaagcga tttctgcaca cgataaacag gccagagtaa ttcgctctcc gctatttctt
     2341 tgccaaagag atattcatca cccggataat ctgcatgtgc gatgacatat tttattatgt
     2401 cgttagtgat ttcaataata tgtggaaggt gatggaattt cagcaacaga tctattgcct
     2461 cttgttgcaa tcatttaaat cgaaaaacca gtcaccaccg ctggcaagcc acatattgcc
     2521 atcgccggtc gcgaacgcat ccagcgccat tgcttcgtgg ttgcctttaa cggagataaa
     2581 ccagggctgg tttagcaggc gcaggacgtc aagactctcc ggcccacgat caatattatc
     2641 gccgacagaa ataagtaagt cggtttcggg gaaaaaagag agttgatgta agcgggattg
     2701 taataactga tattcaccat gaatatcacc aacgacccat atatggcgat agtgatgggc
     2761 attgattttt tggtagcgtg tagatggcat ggttttaccc tgtaaaataa gctttcctat
     2821 tatacagggt atttttattt gattcgtcag ttgtcgttaa tattcccgat agcaaaagac
     2881 tatcgggaat tgttattaca ccagactctt caagcgataa atccactcca gcgcctgacg
     2941 cggagtcagt gaatccgggt cgagattttc cagtgcttcg accgcaggcg aagtttcttc
     3001 tggcactgac agcaaagaca tctgcgtacc atccacttgc gtcgcggcgg cgttcggcga
     3061 aatgctttcc agctcacgca gtttttgccg tgcgcgctta ataacctctt tcggtacgcc
     3121 cgccagagca gcaaccgcca gaccgtagct tttgctcgcc gcgccgtcct gcacgctgtg
     3181 cataaaggcg atagtgtcgc cgtgctccag cgcatcgaga tgtacgttgg cgacgccttc
     3241 cattttctcc ggtagctggg tcagctcgaa atagtgggtg gcaaacagcg tcaatgcctt
     3301 aatcttattc gccagatttt ctgcgcacgc ccacgccagc gacagaccat cgtaggtgga
     3361 cgttccacgc ccgatctcat ccattaacac cagactgtat tcggtggcgt tatgtaaaat
     3421 attggcggtt tcagtcatct ccaccataaa ggttgagcgc ccggaagcca ggtcatctgc
     3481 cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc tcgacttttt gtgccggtac
     3541 atagctgccg atgtaggcca tcagcgcaat cagtgcggtc tggcgcatat aggtactttt
     3601 accgcccatg ttcgggccgg taataatcaa catacggcgc tgcggcgaca gattcagcgg
     3661 gttagcgata aatggctcat tcagtacttg ttcaactacc ggatggcgac cttcggtaat
     3721 gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag ttcagggtat aggcccgttc
     3781 cgccaggtta accagcacgt cgagttccgc cagcgcgctc gcgctctgtt gcaacgcttc
     3841 cagatgcggc aacagcaggt cgaacagctc ttcataaagc tgtttttcca gtgccagtgc
     3901 tttgcctttt gaggtgagaa ctttatcttc gtactctttt agctctggaa tgatgtagcg
     3961 ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg ggtgccagat ggctttgccc
     4021 acggctgatt tgaatgtagt agccgtgcac cgcattaaag ccaactttta gcgtgtccag
     4081 gccggtacgt tcacgctcgc ggacttccag acgctccaga taatcggtcg cgccgtcagc
     4141 cagcgcgcgc cactcatcca gctcttcgtt atagcccgat gcgataacac caccgtcgcg
     4201 taccagcacc ggcggtgtgt cgatgattgc tcgctccagc agatcgcgca gctcggcaaa
     4261 ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta tcgacagttt ctaactgcgc
     4321 acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg gccagatcgc gtgggcgagc
     4381 agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg ccgacctgac gcagtaccgg
     4441 ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt tgctggcgct caagcaacac
     4501 gcgggtatcg cgcactggca tatgcagcca gcgtttcagc atacgactac ccatcggcgt
     4561 gacggtgcag tcgagcacag aagccagcgt attttccgca ccgcccgcca ggttctgagt
     4621 aatttccagg ttacgacgtg tcgcggcatc cataatgatg ctgtcctgct cacgttccat
     4681 ggttatagaa cgaatatgcg gcagggtcgt gcgttgggta tctttcgcat actgcaacag
     4741 acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg ccaaaaccga ccagatcgcg
     4801 ggtgccaaat tgcagattca actgctggcg cgcggtgtcg atttcaaact cccacagcgg
     4861 gcgacggcgc aggccgcgac ggccttcaat cagcgacatc tcggcgaaat cttctgcata
     4921 cagcagttcc gccggattag agcgttgcag ctctgccgcc atcgtttcgc ggtcggccgg
     4981 ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc gcgtagccga aacctttgct
     5041 gtcctgccag atagccgcca gcaggttgtc ctgacgctcc tgtaacaggg cttcatcgct
     5101 gatggtgcct ggcgtaacga tacgcacaac tttgcgctca accggacctt tgctggtcgc
     5161 cggatcgcca atttgttcgc agatggcaac ggactctccc tgattcacca gtttggcgag
     5221 atagttttcc accgcatggt agggaatccc cgccatcggg atcggctctc ccgccgaagc
     5281 accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg cgttttgcgt cgtcataaaa
     5341 cagttcataa aaatcaccca tccggtaaaa cagcaggatc tcgggatgct gggctttcag
     5401 cttgagatac tgctgcatca tgggcgtatg ggcgtcgaaa ttttctattg cactcatggg
     5461 gttatgtccg gttccctgat attaaatggg gtgtgatggt ttttattttc gttgtaatca
     5521 cataaggcgc acatgatacc ggagttaatc atttcagggg aagaagcgaa cggcgagttc
     5581 ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt gcgtgatgct tatgaagcaa
     5641 gtacgcaaaa ttgtatcgat tggcgaaccc ttcttcttta gagtcatagt gccctttaag
     5701 at
//
LOCUS       DADLCQ010000193         5689 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000193 DADLCQ010000000
VERSION     DADLCQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.195"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(23..589)
                     /gene="satP"
                     /locus_tag="JQG95_003831"
     CDS             complement(23..589)
                     /gene="satP"
                     /locus_tag="JQG95_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY5890225.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(738..1451)
                     /gene="msyB"
                     /locus_tag="JQG95_003832"
     CDS             complement(738..1451)
                     /gene="msyB"
                     /locus_tag="JQG95_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY5890226.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(1477..1881)
                     /locus_tag="JQG95_003833"
     CDS             complement(1477..1881)
                     /locus_tag="JQG95_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY5890227.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            2258..4174
                     /gene="dnaK"
                     /locus_tag="JQG95_003834"
     CDS             2258..4174
                     /gene="dnaK"
                     /locus_tag="JQG95_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY5890228.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4263..5393
                     /gene="dnaJ"
                     /locus_tag="JQG95_003835"
     CDS             4263..5393
                     /gene="dnaJ"
                     /locus_tag="JQG95_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY5890229.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(5497..5649)
                     /locus_tag="JQG95_003836"
     CDS             complement(5497..5649)
                     /locus_tag="JQG95_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5890230.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1496 a         1409 c         1495 g         1289 t
ORIGIN      
        1 gagcgggggg atctcaagac aattagtggg attcaccaat cggcagaacg gtgcgaccaa
       61 actgctcgtt cagcacttca cccatcgcca gatagattgc gctggcaccg cagatcagac
      121 caatccagcc ggcaaagtgg atgatcgcgg cattaccggc aatgttaccg atcgccagca
      181 gggcaaacag cacggtcagg ctaaagaaaa cgaattgcag gacgcgcgcg cctttcagag
      241 tgccgaagaa cataaacagc gtaaatacgc cccacagacc caggtagaca ccaaggaact
      301 gtgcatttgg cgcatcggtc agacccagtt tcggcatcag cagaatcgca accagcgtca
      361 gccagaaaga accgtaagag gtgaatgcgg ttaaaccgaa agtgttgcct tttttgtact
      421 ccagcagacc agcaaaaatt tgcgcgatgc cgccgtagaa aatgcccatg gcaagaataa
      481 taccgtccag agcgaaataa cccacgttgt gcaggttaag cagaatggtg gtcatgccga
      541 agcccatcag gcccagcggt gccggattag ccaacttagt gttgcccata attcctcaaa
      601 aatcatcatc gaatgaatgg tgaaataatt tccctgaata actgtagtgt tttcagggcg
      661 cggcataata atcagccagt ggggcagtgt ctacgatctt ttgaggggaa aatgaaaatt
      721 ttccccggtt tccggtatca gacctgagtg gcgctaacca tccggcgcag gcaggcgatt
      781 tgcagtacgg ctggaatcgt cacgcgatag gtgctgccgc tgaccgcttt aaccccattt
      841 aatgctgcgc ccacagggcc tcccagcccc gcgccgcgca gcaaaccatg cccaagtacg
      901 ctcattgctg cgtgggtgcg taaaatgcgg gtcagttggc tggaaagcaa atgcgacaca
      961 ccttttgcca ataatttgtc tttcatcagc agcggcagca gctcttccag ctcattcacc
     1021 ctggcatcga ccgcgtgcag aaactcctgc ttatgttcct cgtccatttt cttccaggta
     1081 ttacgcagaa attgttccag taactgttgc tcaatttcaa acgtagacat ctctttgtcg
     1141 gctttcagct tcaatcgctt tgaaacatcg agcaaaatgg cccgatacaa tttaccgtgt
     1201 ccgcgcagtt tgttggcgat actatcgcca ccaaaatgct gtaattctcc ggcaatcagc
     1261 tgccagttgc ggcgatgttg ctcgggatgc ccttccatcg atttaaacag ttcgttgcgc
     1321 atcagtatgc tggagaggcg agttttgcct ttttcattat gggtgagcaa tcgggcgaaa
     1381 tttgccaact gttcctcact acaatgctga agaaaatcca gatctgaatc attcaggtaa
     1441 ttaacattca ttttttgtgg cttctatatt ctggcgttag tcgtcgccga taattttcag
     1501 cgtggccata tccgatgagt tcaccgtatg accggaaaag gtgatttttg agacgcagcg
     1561 tttattgtcg ttatcgctgt taatgttgat ccagtcagtg gtttgccctt cttttatttc
     1621 tgaaggaata ttcaggctct gactggcgct acgggcggct ttgaaataaa ccgatgcacc
     1681 acttaactgt aaatcgccat ggtcggcaga gagttgtatg cgtttcacaa tgcgacaaac
     1741 aggaagtttc agcgccagat cgttggtttc gttacgcggc attgcaatga cgccgaggag
     1801 tttatggtcg tttgcctgcg ccgtgcagca cagcatcagg ctaatcgcca ggctggcgga
     1861 aatcgtaaaa acggatttca taaggattct cttagtggga agaggtaggg ggatgaatac
     1921 ccactagttt actgctgata aagagaagat tcaggcacgt aatcttttct ttttattaca
     1981 attttttgat gaacgccttg gctgcgattc attctttata tgaataaaat tgctgtcaat
     2041 tttacgtctt gtcctgccat atcgcgaaat ttctgcgcaa aagcacaaaa aatttttgca
     2101 tctccccctt gatgacgtgg gttacgaccc catttagtag tcaaccgcag tgagtgagtc
     2161 tgcaaaaaaa tgaaattggg cagttgaaac cagacgtttc gcccctatta cagactcaca
     2221 accacatgat gaccgaatat atagtggaga cgtttagatg ggtaaaataa ttggtatcga
     2281 cctgggtact accaactctt gtgtagcgat tatggatggc accattcctc gcgtgctgga
     2341 gaacgccgaa ggcgatcgca ccacgccttc tatcattgcc tatacccagg atggtgaaac
     2401 tctggttggt cagccggcta aacgtcaggc agtgacgaac ccgcaaaaca ccctgtttgc
     2461 gattaaacgc ctgattggcc gccgcttcca ggacgaagaa gtacagcgtg atgtttccat
     2521 catgccgttc aaaattattg ctgctgataa cggcgacgca tgggtcgaag ttaaaggcca
     2581 gaaaatggca ccgccgcaga tttctgctga agtgctgaaa aaaatgaaga aaaccgctga
     2641 agattacctg ggtgaaccgg taactgaagc tgttatcacc gtaccggcat actttaacga
     2701 tgctcagcgt caggcaacca aagacgcagg ccgtatcgct ggtctggaag taaaacgtat
     2761 catcaacgaa ccgaccgcag ctgcgttggc ttacggtctg gacaaaggca ctggcaaccg
     2821 tactatcgcg gtttatgacc tggggggtgg tactttcgat atttctatta tcgaaatcga
     2881 cgaagttgac ggcgaaaaaa ccttcgaagt tctggcaacc aacggtgata cccacctggg
     2941 tggtgaagac ttcgacagcc gtctgatcaa ctatctggtt gaagaattca agaaagatca
     3001 gggcattgac ctgcgcaacg atccgctggc aatgcagcgc ctgaaagaag cggcagaaaa
     3061 agcgaaaatc gaactgtctt ccgctcagca gaccgacgtt aacctgccat acatcactgc
     3121 agacgcgacc ggtccgaaac acatgaacat caaagtgact cgtgcgaaac tggaaagcct
     3181 ggttgaagat ctggtaaacc gttccattga gccgctgaaa gttgcactgc aggatgctgg
     3241 cctgtccgta tctgatatcg acgacgttat cctcgttggt ggtcagactc gtatgccaat
     3301 ggttcagaag aaagttgctg agttctttgg taaagagccg cgtaaagacg ttaacccgga
     3361 cgaagctgta gcaatcggtg ctgctgttca gggtggtgtt ctgactggcg acgtaaaaga
     3421 cgtactgctg ctggacgtta ccccgctgtc tctgggtatc gaaaccatgg gcggtgtgat
     3481 gacgacgctg atcgcgaaaa acaccactat cccgaccaag cacagccagg tgttctctac
     3541 cgctgaagac aaccagtctg cggtaaccat ccatgtgctg cagggtgaac gtaaacgtgc
     3601 ggctgataac aaatctctgg gtcagttcaa cctagatggt atcaacccgg caccgcgcgg
     3661 catgccgcag atcgaagtta ccttcgatat cgatgctgac ggtatcctgc acgtttccgc
     3721 gaaagataaa aacagcggta aagagcagaa gatcaccatc aaggcttctt ctggtctgaa
     3781 cgaagatgaa atccagaaaa tggtacgcga cgcagaagct aacgccgaag ctgaccgtaa
     3841 gtttgaagag ctggtacaga ctcgcaacca gggcgaccat ctgctgcaca gcacccgtaa
     3901 gcaggttgaa gaagcaggcg acaaactgcc ggctgacgac aaaactgcta tcgagtctgc
     3961 gctgactgca ctggaaactg ctctgaaagg tgaagacaaa gccgctatcg aagcgaaaat
     4021 gcaggaactg gcacaggttt cccagaaact gatggaaatc gcccagcagc aacatgccca
     4081 gcagcagact gccggtgctg atgcttctgc aaacaacgcg aaagatgacg atgttgtcga
     4141 cgctgaattt gaagaagtca aagacaaaaa ataatcgccc tataaacggg taattatact
     4201 gacacgggcg aaggggaatt tcctctccgc ccgtgcattc atctaggggc aatttaaaaa
     4261 agatggctaa gcaagattat tacgagattt taggcgtttc caaaacagcg gaagagcgtg
     4321 aaatcagaaa ggcctacaaa cgcctggcca tgaaatacca cccggaccgt aaccagggtg
     4381 acaaagaggc cgaggcgaaa tttaaagaga tcaaggaagc ttatgaagtt ctgaccgact
     4441 cgcaaaaacg tgcggcatac gatcagtatg gtcatgctgc gtttgagcaa ggaggcatgg
     4501 gcggcggtgg ttttggcggc ggcgcagact tcagcgatat ttttggtgac gttttcggcg
     4561 atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat ttacgctata
     4621 acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc cgcattccga
     4681 ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca cagccgcaga
     4741 cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg ccagggattc ttcgctgtac
     4801 agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg tgcaacaaat
     4861 gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc ccggcagggg
     4921 tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggcgag catggcgcac
     4981 cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc gagcgtgaag
     5041 gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg ggtggcgaaa
     5101 tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa acccagaccg
     5161 gtaagctatt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc gcacagggtg
     5221 atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgaaaag cagaaacagc
     5281 tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac agcccgcgct
     5341 caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc taacctcccc
     5401 aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat atgcgagcac
     5461 ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt aagccgtgaa
     5521 aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt ttctcgttac
     5581 cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca tcgccttatg
     5641 ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcact
//
LOCUS       DADLCQ010000194         5683 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000194 DADLCQ010000000
VERSION     DADLCQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.196"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            873..3158
                     /gene="nrdA"
                     /locus_tag="JQG95_003837"
     CDS             873..3158
                     /gene="nrdA"
                     /locus_tag="JQG95_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY5890231.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3392..4522
                     /gene="nrdB"
                     /locus_tag="JQG95_003838"
     CDS             3392..4522
                     /gene="nrdB"
                     /locus_tag="JQG95_003838"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY5890232.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4522..4776
                     /gene="yfaE"
                     /locus_tag="JQG95_003839"
     CDS             4522..4776
                     /gene="yfaE"
                     /locus_tag="JQG95_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY5890233.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4830..5480)
                     /gene="inaA"
                     /locus_tag="JQG95_003840"
     CDS             complement(4830..5480)
                     /gene="inaA"
                     /locus_tag="JQG95_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY5890234.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
BASE COUNT         1456 a         1487 c         1350 g         1390 t
ORIGIN      
        1 gatactccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
       61 gatatcatat ccctggcata aatcaatggc cgccgcgaca ccgttagcag agaaaagaga
      121 tgcaatcatt gacgggagta gagataaata ctccttgcat agaaagataa tccgcatatt
      181 aatctgacca tcattttata taagacggca tatttaaacg cattatacaa tatgggttat
      241 ctttcttttg taaattgtcc aacccgatgc aatggctatt ttgttattat ttgatgaata
      301 atatcagtgc gtcataattc aagttaataa ccttaaggga taccagttat atttaaacta
      361 aattaaagta atgaataatt ttcttataat ataaggtaaa ttaacaaaat ggcttcgcat
      421 ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
      481 tatattgacc acaactgata catcagatta tgtgatgact cgtgcttaga tcaatttttg
      541 caatcattag caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
      601 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
      661 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
      721 agttatgcac ttgcaagagg gtcattttca cactatcttg cagtgaatcc caaacatacc
      781 ccctatatat agtgttctaa gcagcttccc ttactacagg tagtctgcat gaaactattg
      841 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
      901 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
      961 cagaaggact gcataacgtt tcgatttccc aggtcgagct gcgctcccac attcagtttt
     1021 atgacggtat caagacctct gacatccacg aaactattat caaggctgcc gcagacctga
     1081 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcgatc ttccacctgc
     1141 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
     1201 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
     1261 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
     1321 agctggaagg caaatatcta gtacagaacc gcgtgaccgg cgaaatctac gaaagcgccc
     1381 agttccttta tattcttgtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
     1441 tgcaatacgt gaagcgtttt tatgacgcgg tttccacatt taaaatttcg ctgccgacgc
     1501 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
     1561 gcggtgacag cctggattcc atcaacgcca cctccagcgc gattgtgaaa tatgtttccc
     1621 agcgtgccgg gatcggcatc aacgccggac gtattcgtgc gctgggcagc ccgattcgcg
     1681 gtggcgaagc gttccatacc ggctgtatcc cgttctacaa acatttccag acagcggtga
     1741 aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac gctgttctac ccgatgtggc
     1801 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggggttgaa ggcaaccgcg
     1861 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgc ctgctgaaag
     1921 gtgaagatat caccctgttc agcccgtccg acgtaccggg gctgtacgac gcgttcttcg
     1981 ccgatcagga agagtttgaa cgcctgtata ccaaatatga gaaagacgac agcatccgca
     2041 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
     2101 gtcgtatcta tattcagaac gttgaccact gtaataccca tagtccgttt gatccgacca
     2161 tcgcgccagt tcgccagtct aacctgtgcc tggaaattgc cctgccgacc aaaccgctga
     2221 acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
     2281 gcgcaattaa tagcctggat gaactggaag agctggcgat tctggcggtt cgtgcacttg
     2341 acgcgctact ggattatcag gattacccga tcccggccgc caaacgtgga gcgatggggc
     2401 gtcgtacgct gggtattggt gtgattaact tcgcttacta tctggcgaag cacggtaaac
     2461 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcg attcagtatt
     2521 acctgctgaa agcgtctaat gagctggcta aagagcaagg cgcgtgcccg tggtttaacg
     2581 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatgcca
     2641 tcgctaatga gccgctgcat tacgactggg aagcattgcg tgaatcaatc aaaacgcacg
     2701 gcctgcgtaa ctccacgctt tctgccctga tgccgtccga gacctcttcg cagatctcta
     2761 acgccactaa cggtattgaa ccgccgcgcg gttacgtcag catcaaagcg tcgaaagacg
     2821 gtattttgcg ccaggtggtg ccggactacg agcacctgca cgacgcctat gagctgctgt
     2881 gggaaatgcc gggtaacgat ggctacctgc aactggtagg tatcatgcag aaatttatcg
     2941 atcagtcgat ctctgctaac accaactacg atccgtcacg tttcccgtca ggaaaagtgc
     3001 cgatgcagca gttgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
     3061 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
     3121 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
     3181 taaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
     3241 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     3301 taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     3361 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     3421 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     3481 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     3541 tccggaagaa gttgacgtct cccgcgaccg tatagattac caggcgctgc cggagcacga
     3601 aaaacacatc tttatcagca acctgaaata tcagacgctg ctggattcca ttcagggtcg
     3661 tagcccgaac gtggcgctat tgccgcttat ttctattccg gaactggaaa cctgggtcga
     3721 aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     3781 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     3841 tgcggaaggg atctccagct attacgatga actgatcgaa atgaccagct actggcacct
     3901 tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta accgttagcc tgcgcgagct
     3961 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga tccgcttcta
     4021 cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag gtaacgccaa
     4081 gattattcgc ctgattgccc gcgatgaagc cctgcacctg accggtactc agcatatgct
     4141 gaatctgctg cgcagcggcg cggatgatcc ggagatggcg gaaattgccg aagaatgtaa
     4201 gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact gggcggatta
     4261 tctgttccgt gacggttcga tgattggtct gaataaagat attctctgcc agtacgttga
     4321 atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     4381 caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     4441 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcagaagtgg acaccgacga
     4501 tttgagtaac ttccagctct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     4561 ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgtggcggtt
     4621 gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cccgactggt cgcaggtcag
     4681 gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     4741 tgccgggcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc cagatacgac
     4801 gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     4861 tcccagtccg ctttagggat cggctccaga tatttttcca gctggcggaa gtcatgatta
     4921 atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa accagcttct
     4981 gccttacctt ctgttttcac atagatatgg cgaacatagc aacagccatg ctgacgatta
     5041 acgctatgca ttttcttaaa cgccagtgcc actgctttca acatggcttg ccgtacttcg
     5101 tcagaataag gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     5161 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     5221 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     5281 acaatcgttg gtcggccgaa cggataacgt actgaatgaa acagatgatg cgtcatgcgc
     5341 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     5401 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5461 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     5521 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccgc
     5581 tacaggtgtg acaccgcttt tgcccttcat ttgcagcgca tagattccac cagccactac
     5641 cactaatagt gcgcccagca gataaaatgt gaatttaatc agt
//
LOCUS       DADLCQ010000195         5643 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000195 DADLCQ010000000
VERSION     DADLCQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.198"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(27..398)
                     /locus_tag="JQG95_003841"
     CDS             complement(27..398)
                     /locus_tag="JQG95_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HAY5890235.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTD"
     gene            923..1897
                     /gene="hemB"
                     /locus_tag="JQG95_003842"
     CDS             923..1897
                     /gene="hemB"
                     /locus_tag="JQG95_003842"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY5890236.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2003..2854)
                     /gene="tauD"
                     /locus_tag="JQG95_003843"
     CDS             complement(2003..2854)
                     /gene="tauD"
                     /locus_tag="JQG95_003843"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY5890237.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2851..3678)
                     /gene="tauC"
                     /locus_tag="JQG95_003844"
     CDS             complement(2851..3678)
                     /gene="tauC"
                     /locus_tag="JQG95_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY5890238.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3675..4442)
                     /gene="tauB"
                     /locus_tag="JQG95_003845"
     CDS             complement(3675..4442)
                     /gene="tauB"
                     /locus_tag="JQG95_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY5890239.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4455..5381)
                     /gene="tauA"
                     /locus_tag="JQG95_003846"
     CDS             complement(4455..5381)
                     /gene="tauA"
                     /locus_tag="JQG95_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5890240.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1380 a         1528 c         1410 g         1325 t
ORIGIN      
        1 gttgtctgga gattcagggg gccagtctaa tcagttgtca tgcactcgga ataaatcata
       61 ccgtgaatag tgctgttttt gattgtcaga ctattggcgt tggtattggt agaaataccg
      121 tcgtccagat agtaggtaga aatcacgccg ttaacggtag cgttattaat gaccgggtag
      181 atatcgccgt tgtaatagct atcagcggca tagttgttcc agcctacata accatcatag
      241 taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta agtttgacca
      301 gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca agccaatgct
      361 aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc ctcagggacg
      421 taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc gcgctaacga
      481 ttatgctgcg ttacagacga atggttcaat tattctttcc cctaagtccg aatgtggaca
      541 tatataattt atggagaaaa acacgcatca aattgataaa aggaagatat ttcagtatgt
      601 tgcagataat gttatttttg atgcgtacgc gttataaata tcaaaaacac aaaccagata
      661 aatatcttat caatatgcaa caaagtttga attattcaat ttatagcagc acaaccaaac
      721 gccaaaaaaa taataaccaa gagctataaa taaagattaa aaatattttc agtgaatatg
      781 aaattattgc tatttctatt acaaagatgt cctggtctct ctttcattct cttcttgtca
      841 acatcgcgac aactttcgta aaacactcct gccccgcttc aggtatactg tgcaccttaa
      901 ttccacaaac atcaggcaga ccatgacaga cttaatccaa cgccctcgtc gcctgcgcaa
      961 atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt agccttaacg acctggtgtt
     1021 gccgatcttt gttgaagaag aaattgacga ctacaaagcc gttgaagcca tgccaggcgt
     1081 gatgcgcatt ccagagaaac atctggcacg cgaaattgaa cgcatcgcca acgccggtat
     1141 ccgttccgta atgacttttg gcatctctca ccataccgat gaaaccggca gcgacgcctg
     1201 gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc aagcagactg tgccagaaat
     1261 gatcgtcatg tcagacactt gtttctgtga atacacttct cacggtcact gcggtgtgct
     1321 gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc aagccgtggt
     1381 tgcagctgct gcaggcgcag acttcatcgc cccttccgcc gcgatggacg gccaggtaca
     1441 ggcgattcgt caggcgctgg acgctgcagg ctttaaagat acggcgatta tgtcgtattc
     1501 gaccaagttc gcctcttcct tttatggccc gttccgtgaa gctgcaggaa gcgcattaaa
     1561 aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt cgcgaggcga ttcgtgagtc
     1621 actgctggat gaagcccagg gcgcagactg cctgatggtt aaacctgctg gagcgtacct
     1681 cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt atcaggtgag
     1741 cggtgagtac gcgatgatta agttcgccgc gctggcgggt gctatagatg aagagaaagt
     1801 cgtgctcgaa agcttaggtt caattaagcg tgcgggtgcg gatctgattt tcagctactt
     1861 tgcgctggat ttggctgaga agaagattct gcgttaattt ttttcctctc agaaaggggg
     1921 agttaacctg ttgaaagtac agtgaacgcc ccctcaccct aaccctctcc ccaagggggc
     1981 gagggaaccg tccgcactcg tatcaccccg cccgataaaa cggtttatcc ccaaggatcg
     2041 tcgcccgatg cataatccgc cgctgtggca ggtaatcggc attggcatag tgctgagtca
     2101 cgcggttatc ccaaatcgca atatcattcg gttgccagcg ccaacgcacc tgaaactccg
     2161 gtttagtgat atgggcgaat aaaaagccta acaaggcttc gctctctttc tcgctgacat
     2221 cgacaattcg cgtagtaaag ccttcattca caaacagcgc ctgtttaccg ctcaccggat
     2281 gcgttcgcac caccggatgt agcaacggag gatttttcgc gaccgcctcg cgccagcgtt
     2341 gatgttcctc ctcggttttg cggtatttgt attccgggaa tgatttacgg aaatcatgct
     2401 ccgcacgcag ccagctcagc agctggcgga agggaacaga gagcgcctca taggccgcaa
     2461 taccgctggt ccagagcgta tcaccgccgg tcgaaggtaa ctctttagct gccagaatcg
     2521 cccctgcggg tggcgtttca ataaatgtca catcggtatg ccagttgtcg ttatccggcg
     2581 gattatcgtt atgggtatcc agcacgatga tctcgtcaac cccttcggca tgagggtaaa
     2641 cagggtgaat atgcagttcg ccaaaacgct gggccagcgc gcgttgctgc tgcggcgtaa
     2701 tagcttgatc gcgcagaaat accacctgat ggcgcagcac cgcatggtaa agctgttcaa
     2761 actgattatc gcttaacggg cgcgtcaggt cggcacccga aatttgtgcg ccgatatacg
     2821 gccccagcgg ggtaatgctc agacgttcac tcattgtact tctccatgcc agggcgtcag
     2881 acggcgctgt aacgcgcgca gacccagttc taaaagaaag gcgataatcg caatcaccgc
     2941 gatccccgcc agtaccacgt cagtcgcgag aaattcaccc gctgactgaa ccataaatcc
     3001 taaaccgcgt gtcgcggcaa tcagctccgc cgctaccagc gtagaccagc ccacacccag
     3061 accaatgcgt aatccggtga ggatttccgg cagcgcgccg ggcaaaatga caaaccacag
     3121 cacctgcgca cggctggcac ccagcgactg ggcggcacga atgcgaacct gctgcgcgct
     3181 tttcacccct gccagcgccg acatcgccac cggcgcaaaa atcgctaaat agatcagtaa
     3241 gatcttcgag gtttcaccaa taccaaacca gatcaccatc agcggcaaat aagccagcgg
     3301 tggcaccgga cgataaagtt cgattaccgg atccagaatg ccgcgcaccg tcgggcttag
     3361 ccccatggcg atcccgaccg gaataccgat caccaccgcc gcgagtagcg ccagcacaat
     3421 gcgcgtcagg ctggctgcca gatgctgcca cagcgtggcg tccataaatc cctgcggtcc
     3481 ggcaatggtg agcagttttg ccagtacttg ttgcggcggc ggcaaaaaca gcgggctaat
     3541 cagttgcagc gccgccaccg tccaccatac agtgagtaaa accgctaacg tgccaatgct
     3601 taaggtcacc tgacgcgaga gcggccagcg ccatttcagc cgccgcgaat gcagtttttc
     3661 attaatgagc acactcatga gaacgcctcc cgttgctcaa atacgcggct taaaacatat
     3721 tcgcgcatgg cgatgaattg tggatcggac ttgatgctgc ggctcgactc tcccgcaaca
     3781 aagcggcgag caaagttgag ccgcagccgc tccagcacac ggccagggcc ggatgaaagc
     3841 agaaccagtt cagtcgccat aaacaccgct tcttctatat cgtgggtaat caacagcacc
     3901 tgcttgcccg tctcctgcca gagtttcagc agcagagttt gcatctggtc gcgggtgaag
     3961 gcgtccagcg caccaaacgg ttcgtcgagt aataacagct ggggattcgc cgccagcgcg
     4021 cgggcaatcc ccacccgctg acgttgacca tcggaaagct gccagatata gcgtttttct
     4081 gcgccttcca gccccacttt tttcagcatc tggtgcgcga tttccagtcg ctgcattttc
     4141 tctatacctg ccaattgcag gccgaacgcc acgttgtcct gtacattgcg ccacggtagc
     4201 agcccttcat tctgaaaaac tacgccacgc tctgctcccg gtccctcaat acgcttaccc
     4261 gccagttgaa tgctgccatg ctgataaggc acaaaaccgg caatcagatt cagcagggtg
     4321 gttttaccgc agccggacgg ccccagcacc accagtagct cgccgctttc cagcgtcagg
     4381 ttgatatctt ccagtgccgg tttgccgcca taatcggcat agagatgaga gatttgcagc
     4441 atctgcgcct ccttttattg cacgaagcgc gaggtaacgt actggctgta atcattcgct
     4501 acagccggga ccttgccctg ctctttcaaa aactgcgcgg tgtcgatgat tgctttgttc
     4561 accggtccgg tcagttctgc cgtttgttgc tgcggcgtca gataggtatt tcccttcacc
     4621 agccccggaa cgtcaccttc aggcacgccg cttaaacgcg ccagtttgct gatgttttcc
     4681 ggctgtttca gccacgcgtc agggttagca atgtacggtt gctgtgcatc gatggcgctt
     4741 ttggcgaacg ctttcacgac ctcaggatgt ttctcggcaa aatctttgcg caccacccag
     4801 acgtccagcg ttggcgcacc ccactgcccg acctgttcag aatcggtcag caccttgccg
     4861 tctttttcca gggcgttaac cgccggtgcc cagacataag caccatcaat atcaccccgc
     4921 tgccatgcgg cgataatcgc gggcggctgc aggttcacaa tctccactag ccctggttta
     4981 atcccccagt gtttcagcgc cgccagcagg ctgtagtggg tggtggagat aaacggtacg
     5041 gcaatgcgtt taccaatcaa atcttcgggt ttgctgatag ttttctttac caccagcgct
     5101 tcggagttac ccagttttga cgccagcaag aagacttcaa tcggcacctg ttggctggct
     5161 gcaaccgcta atgggctgga accgaggttg ccgatttgca cgtcgccaga agccagcgcc
     5221 cgcacgatgc tggctccgct gtcaaactta cgccagtcca cgctcgcccc gctctcttta
     5281 gcaaaggtgt tgtcggcctg agccactttc gccggttcgg ctgaggtttg atacgccacg
     5341 gtgacgttca ccgcctgtgc ctgaaaagcg atgaatgcca gtgcggcaag aagtgtgtta
     5401 tatgactagg taatttattc aagataaccg ggaatgggta aaaaaagacg ggatttgatg
     5461 ggattcaata agttatgcgc cgattgcatt caagataaaa cgagttaaaa atgctcaatg
     5521 cttcgatatc gataaaacat tcaagatagg cgcgccgtgt attttaattc ttttaagact
     5581 cttcaaaaat gtcatcgtag cctttaaaaa cgaattttaa aggcacccta aaggcaggtc
     5641 aat
//
LOCUS       DADLCQ010000196         5601 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000196 DADLCQ010000000
VERSION     DADLCQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.199"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..411
                     /locus_tag="JQG95_003847"
     CDS             <1..411
                     /locus_tag="JQG95_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5890241.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /locus_tag="JQG95_003848"
                     /pseudo
     CDS             443..1057
                     /locus_tag="JQG95_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="JQG95_003849"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="JQG95_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY5890242.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1331 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tagtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggagg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DADLCQ010000197         5581 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000197 DADLCQ010000000
VERSION     DADLCQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.200"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            28..243
                     /locus_tag="JQG95_003850"
     CDS             28..243
                     /locus_tag="JQG95_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY5890243.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            236..622
                     /locus_tag="JQG95_003851"
     CDS             236..622
                     /locus_tag="JQG95_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY5890244.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            630..1316
                     /locus_tag="JQG95_003852"
     CDS             630..1316
                     /locus_tag="JQG95_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY5890245.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="JQG95_003853"
     CDS             complement(1294..1917)
                     /gene="tetR(B)"
                     /locus_tag="JQG95_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY5890246.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1999..3204
                     /gene="tet(B)"
                     /locus_tag="JQG95_003854"
     CDS             1999..3204
                     /gene="tet(B)"
                     /locus_tag="JQG95_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY5890247.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="JQG95_003855"
     CDS             complement(3317..3910)
                     /gene="tetC"
                     /locus_tag="JQG95_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY5890248.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3998..4408
                     /locus_tag="JQG95_003856"
                     /pseudo
     CDS             3998..4408
                     /locus_tag="JQG95_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4424..>5581)
                     /locus_tag="JQG95_003857"
     CDS             complement(4424..>5581)
                     /locus_tag="JQG95_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY5890249.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1030 c         1215 g         1794 t
ORIGIN      
        1 ccactattaa gtcatgtagc tggttttatt tcaattgaac gttttcaaag tctagctaca
       61 gaaggaaaaa tgttatcgct atcttggtgg gaaaacgaat acgcagttct gcaatggaaa
      121 aatcatgttt tacatgcgaa agctcaacaa gaagggcgag agtcaatatt tgatttttac
      181 aaaattagta ttgctcatat tactcgcgaa tattcattta aaaaggacaa ggataatgtt
      241 tgatgttcac gttgttttag ataatcaaat aggacaatta gcattactag gaaaaacatt
      301 aggtaataaa ggtattggat tggaaggggg agggatattt acggttggtg atgaatgcca
      361 tgctcatttt cttgttgaac aaggaaagga agctaaaata gcgctagagc aagctggact
      421 gttagtactt gcgatccgga caccattaat tcgtaagtta aaacaggaaa aaccggggga
      481 acttggcgaa atagcacgag tattggcgga gaataacatt aatattttag tgcaatacag
      541 tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg catctgttac
      601 gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa gaggttacct
      661 ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga gttaaaatac
      721 tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct gtggcgaata
      781 tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag ctaatcacag
      841 tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt gctgaattga
      901 tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt tccacgccag
      961 tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt gccgttaaga
     1021 tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg atcacattaa
     1081 gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat tcacgtaaaa
     1141 tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg tacgttggag
     1201 ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt ggttaccgtg
     1261 aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac atttaagttg
     1321 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1381 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1441 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1501 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1561 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1621 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1681 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1741 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1801 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1861 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1921 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
     1981 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
     2041 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
     2101 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
     2161 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
     2221 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
     2281 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     2341 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     2401 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     2461 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     2521 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     2581 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     2641 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     2701 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     2761 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     2821 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     2881 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     2941 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     3001 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     3061 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     3121 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     3181 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     3241 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     3301 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     3361 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     3421 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     3481 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     3541 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     3601 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     3661 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     3721 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     3781 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     3841 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     3901 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     3961 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     4021 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     4081 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     4141 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     4201 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     4261 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgattccca
     4321 acaatctttt acacgtcgat ttaagtacat ttttaaggtt acaccaagtt attatcggcg
     4381 taataaatta tgggaattgg aggcaatgca ctgagagatc ccctcataat ttccccaaag
     4441 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     4501 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     4561 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     4621 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     4681 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     4741 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     4801 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     4861 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     4921 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     4981 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     5041 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     5101 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     5161 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     5221 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     5281 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     5341 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     5401 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     5461 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     5521 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     5581 a
//
LOCUS       DADLCQ010000198         5559 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000198 DADLCQ010000000
VERSION     DADLCQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.201"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..641
                     /locus_tag="JQG95_003858"
     CDS             <1..641
                     /locus_tag="JQG95_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890250.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQG95_003859"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="JQG95_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY5890251.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="JQG95_003860"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="JQG95_003860"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY5890252.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1379 a         1359 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatag
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaaaa
//
LOCUS       DADLCQ010000199         5480 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000199 DADLCQ010000000
VERSION     DADLCQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.202"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            246..767
                     /locus_tag="JQG95_003861"
     CDS             246..767
                     /locus_tag="JQG95_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY5890253.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQTQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            824..1057
                     /locus_tag="JQG95_003862"
     CDS             824..1057
                     /locus_tag="JQG95_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY5890254.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGAHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1118..3067
                     /locus_tag="JQG95_003863"
     CDS             1118..3067
                     /locus_tag="JQG95_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY5890255.1"
                     /translation="MSVAKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVEVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDAADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQKPDKSL
                     ALLAWTLCLNVFGSGAYSNPARIRLECEHYSLTSDAPSGKEGAAFMALMAEHSRFAAL
                     LPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAIGFH
                     MRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEYAEHHMKDNRW
                     VPGWMCAPRPQTDTTERPDNLADAA"
     gene            3122..3556
                     /gene="psiB"
                     /locus_tag="JQG95_003864"
     CDS             3122..3556
                     /gene="psiB"
                     /locus_tag="JQG95_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAY5890256.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
     gene            3553..4272
                     /locus_tag="JQG95_003865"
     CDS             3553..4272
                     /locus_tag="JQG95_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAY5890257.1"
                     /translation="MSARSQALVPLSTEQQAAWQAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGSRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLTGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4269..4583
                     /locus_tag="JQG95_003866"
     CDS             4269..4583
                     /locus_tag="JQG95_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HAY5890258.1"
                     /translation="MKWCYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4792..4953
                     /locus_tag="JQG95_003867"
     CDS             4792..4953
                     /locus_tag="JQG95_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5890259.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTA
                     TMAYESGGK"
     gene            complement(5254..5480)
                     /locus_tag="JQG95_003868"
                     /pseudo
     CDS             complement(5254..5480)
                     /locus_tag="JQG95_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1207 a         1456 c         1719 g         1098 t
ORIGIN      
        1 actgtccagg gtaaatgaac agcccacaaa attttttacc caaaacccgg taaaaaattt
       61 tgcgttctgc ccaggacagg tgcgtcaggc cgtggcagtg atgccccatg cggcgaaggt
      121 tctcaaacag gtgatggtga ataccggagg gaacaaaagg caggtccccg caaaacgtca
      181 aaaccgcggc tcccgaaact caacagatga tgggggctga aaacatcaac agaaggagat
      241 acatcatggc agttcgtggc attaacaagg tcattcttgt cggtcgtctg ggaaaagatc
      301 cggaagtccg ttacatcccc aacgggggcg cagtggcaaa cctgcaggtg gccacgtcag
      361 aaagctggcg tgacaaacag acgggggaga tgcgggagca gacagaatgg catcgcgtgg
      421 ttctgttcgg caagctcgcg gaagtggctg gcgaatatct gcgcaagggc gtgcaggtct
      481 acatcgaagg tcagcttcgc acccgtagct gggaagataa cggtatcacc cgttacgtca
      541 ccgaaattct tgttaagacc acaggcacca tgcagatgct ggggagtgca ccacagcaga
      601 acgctcagac gcaaccggag cctcagcaga atgggcagcc acagagtgct gacgcgacga
      661 aaaaaggtgg cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagccc gagcctcagc
      721 agccgtctga acccgcgtat gacttcgatg acgacattcc gttctgaacg actgacagtg
      781 acaaccgccc cgtccagtgc ggggcatcac cggagagatg aggatgagcg aatatttcag
      841 aatatttcag gggctgcctg acggctcctt tacccgcgaa caggctgaag ccgttgccgc
      901 acagtaccgg aatgtcttta tcgaggatga tcagggagcg cattttcgcc tggttgtccg
      961 caataacagt ggcctggtct ggcgtacctg gaattttgag gacggtgccg ggtactggat
     1021 gaaccatgtc atccggaatt tcgggatcct taagtaagag aggtgccgga cggggatcac
     1081 gtccggcaga cattaactac acaataaggg gatagtcatg tcagtagcaa agtctgaacc
     1141 aaaatctacc cgtaaagcaa gccgtaaacc tgcaaaaacg caggaaactg tcctgtctgc
     1201 cctgctggcg cagacggagg aagtgagcgt gccgctggcc tcactgatta aatcaccgct
     1261 gaatgtgcgc acggtgccgt attctgcgga gtccgtcagc gaactggcgg actccattaa
     1321 aggtgtcggc ctgttacaga atctggttgt tcatgccctg ccaggtgacc gttacggtgt
     1381 cgccgcaggc ggtcgccgac tggcagcact caacatgctg gcagagcgca acatccttcc
     1441 ggctgactgg cctgtacgcg tgaaggtcat tccgcaggag ctggcgactg ccgcatcgat
     1501 gaccgagaac ggtcagcgtc gggatatgca ccctgccgaa cagattgccg gattccgcgc
     1561 aatggcgcag gaaggcaaaa cacctgcgca gactggcgac ctgctgggct attcaccccg
     1621 ccacgtccag cgaatgctga aactggcaga ccttgcgcct gtcatcctcg atgcgctggc
     1681 agaagaccgc atcaccaccg aacactgtca ggcgctggcg ctggagaacg acaccgcgcg
     1741 tcaggtggag gtgtttgaag ctgcctgtca gtcgggatgg ggcggaaaac cggatgtgcg
     1801 ggttatccgc aatctgatta cggaaagtga agtggcggtg gcggggaaca gtaaattccg
     1861 cttcgtgggg gctgatgcct tctcgccaga cgaactgcgc accgatttgt tcagcgacga
     1921 cgagggtggc tatgtggact gcgtggcgct cgatgccgcc ctgctggaaa aactccaggc
     1981 tgtcgcagag ttccttcggg aagccgaagg ctgggaatgg tgcgccggac gcatggagcc
     2041 tgtcggtgag tgccgtgagg atgccggaac ataccgctgt ctgccggagc cggaagcggt
     2101 gctgaccgag gcggaagaag aacgtctgaa cgaactgatg gcacgttacg acgcgctgga
     2161 aaaccagtgt gaggaatctg acctgctgga agcagaaatg aagctgatgc gctgcatggc
     2221 gaaggtcaga gcgtggacgc cggagatgcg tgccggaggt ggagtggtgg tgtcctggcg
     2281 ttatggaaac gtatgtgtcc agcgtggtgt gcagttgcgc agtgaagatg acgctgctga
     2341 ccgcacggaa caggtgcagg agaaagcatc agtggaggaa atcagtctgc cgttgctgac
     2401 gaaaatgtcc tcagagcgca cgctggcagt ccaggcagca ctgatgcaga agccggacaa
     2461 atccctggca ctgctggcat ggacgctctg cctgaatgtg tttggcagtg gggcgtacag
     2521 caatcccgcc agaatccgcc tggaatgtga gcattattcg ctgaccagcg atgcgccgtc
     2581 ggggaaggaa ggtgccgcat tcatggcgct gatggcagaa cactctcgtt ttgctgccct
     2641 gctgccggaa ggatgggaac gggatatgac gacgttcctg tcactcagcc aggaggtgct
     2701 gttatccctg ctcagtttct gtaccgcgtg cagtcttaac ggtgtccaga cccgcgagtg
     2761 tggtcacacg tcacgcagtc cgcttgactc gctggaaagc gccatcggct ttcacatgcg
     2821 cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc cgcagattat
     2881 cgcagccctg aatgaggcag ggctgtccgg tgccgcacgg gacgcggaga agatgaagaa
     2941 aggtgatgcg gctgaatatg cagagcacca tatgaaagac aaccgctggg tgccaggctg
     3001 gatgtgtgca ccacgtccac agacggatac cactgaacgc cctgataacc tggctgatgc
     3061 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg gagataccgt
     3121 gatgaaaact gaactgaccc tgaatgtatt acagaccatg agcgcacagg aatatgaaga
     3181 tatccgggct gccggaagtg atgaacgccg tgagctgaca cacgccgtga tgcgggaact
     3241 ggatgcgccg gacaactgga cgatgaacgg cgagtacggc agcgagtttg gcggattttt
     3301 ccccgtccag gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc
     3361 gggcgatgtc tctcaggtct gggtgctggt tctggtgaat gccggtggtg agccgtttgc
     3421 ggtggttcag gtacaacggc ggtttgcacc ggaagccgtc agccattcac tggcactggc
     3481 ggcatcactg gatgcgcagg ggtacagcgt taacgacatc attcatatcc tgatggcgga
     3541 aggaggtcag gtatgagcgc acgttcacag gcactggttc ccctcagcac agagcagcag
     3601 gccgcatggc aggcggtggc ggagacggaa aaacgccgcc atcagggtaa cacgctggcg
     3661 gaatatccgt atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatttcgctg
     3721 tctgacctgc gatttttcat gccatccctg accgcagaag aactgcacgg tagccgtctt
     3781 cagtggctgt atgccatcga tgtgctgatt gaaacacagg gcgaggtctg cctccttcct
     3841 cttccaggcg atgcagcaga acgcttgttc ccgtcggtgc ggttccgtgt cagggaacgc
     3901 agtcgccata aatccgcact ggtcatgcag aagtacagcc gtcagcaggc gagggaggca
     3961 gagcagaaag cccgtgcgta ccaggcactg gtcgctcagg ctgaaatcga actggcattc
     4021 cattcgccgg aaacagtcgg aagctggcat gcacgctgga gtgaccgggt ggcagagcat
     4081 gacctggaaa ccctgttctg gcagtggggc gagcgttttc cgtcactgac cgggatggaa
     4141 cgctggcagt ggcaggatat gccattctgg caggtcatcg cagaggctgg tatggcggca
     4201 agggaggccg gtcatgccgt ccgtgagatg gagcgatgga tggtgccgaa caaactccgg
     4261 gaggcggcgt gaagtggtgt tactcactgc gctggaaact gccttatccc tgtcctggcg
     4321 agcatgaact ggtttcagaa gtggtggagg cggggcagcc tgctccggca tccgttatgt
     4381 cacgctgggt tgccggagca gggtatgcgg tctgtctgga ttttatttct gaccgcccgg
     4441 tgcgccgctg gagcgaagaa cgtaaggctg cggtacgacg gcggaatctg gagaaacgta
     4501 tcaacaggca tgcaccactg tttgcggatg aactgatagc ccgtgaactg gctgaacgac
     4561 cggattattt tcagggaaag tgagtgtggt cagcgtgcag ggatatgagc tatgatgtgc
     4621 ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttaccgt gttgtgtggc
     4681 agaaggcaga aagccccgta gttaattttt cattaaccca cgaggccccc tgtatgtcta
     4741 accaacacca gtatggcctc ttaccgtgca aattgcaagg aggagtaaac catgaaacta
     4801 ccgggaaacg cccttatctg gtgcgtatta atcgtgtgct gcacgttgtt gatattcaca
     4861 ctcctgaccc ggaatcgcct gtgcgaagtc cggctgaagg acggatacag ggaagttacg
     4921 gcaacaatgg cttacgaatc cggcggtaag tagcaacctg gaggcgggcg caggcccgcc
     4981 ttttcagggc tgatgctggt ctgactgcac tgaagcgcct ttataaaggg gctgctggtt
     5041 cgccggtggc ccctttctcc ttgctggcat tgtgcgggca tgaacaacca gactgccagg
     5101 aaggccgttt ccctccggga acggggaaga acgcgcgcct gcggcgcccg ttcttttccc
     5161 ccgccttctc tgtttatgac ctgtcagaaa tcacatcacc ttccctttga ttattctctt
     5221 tttcctcctg ttctgactct gattactgct gccctgatta ttgtctgttt tgttgttctc
     5281 ctggctgtgt cttttccggg cgtccccctg gcgggcttcc atccctgacg cgaaaccccg
     5341 ggcttgcagc gaccgttccg gtcgcgccgc ctctgtcgcg ccgtggcctg cgctcccggc
     5401 atcccggcaa aaggccgccg ggatgtaagg cgcaacgccg tttcgcaaac cgcgcttcac
     5461 gtcgttgcgg cgggtccctg
//
LOCUS       DADLCQ010000200         5400 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000200 DADLCQ010000000
VERSION     DADLCQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.203"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..497
                     /locus_tag="JQG95_003869"
     CDS             <1..497
                     /locus_tag="JQG95_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY5890260.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="JQG95_003870"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="JQG95_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="JQG95_003871"
     CDS             2500..3114
                     /locus_tag="JQG95_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY5890261.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="JQG95_003872"
     CDS             3114..3443
                     /locus_tag="JQG95_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY5890262.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="JQG95_003873"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="JQG95_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY5890263.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVTSGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4530..4841
                     /locus_tag="JQG95_003874"
     CDS             4530..4841
                     /locus_tag="JQG95_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5890264.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4825..4983
                     /locus_tag="JQG95_003875"
     CDS             4825..4983
                     /locus_tag="JQG95_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY5890265.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4976..5374
                     /locus_tag="JQG95_003876"
     CDS             4976..5374
                     /locus_tag="JQG95_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5890266.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1181 a         1342 c         1424 g         1453 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaatac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa actatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtgacgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 tttttattgt ctgagatcag agatacggct aataaccgta tctcttgaca gagtgagcag
     4441 atgcacataa attaatcaaa cggttgatag ccgtattatg tatgagatcg actccctacg
     4501 atttcattca gttaatcaaa gaagtcataa tgcctggaaa aacagccgct ctatacgatg
     4561 tcgataaaac gctgaaaaat gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca
     4621 aacttcgtga agctgcgcaa gccgttgggg tagatttaag cgcttttatt ttgagcgctg
     4681 ctatggaacg cgccgaaagt gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa
     4741 gctgggaact gatgaaccaa ctcatcgctg aacctgctca accgacgctc gccctcaagg
     4801 cgttaatgaa aaggaaaaac agcgatggtc gacaagcatg aagagattac tctgcccata
     4861 gtcctctcct gtaattatca gtctgatatt acttatcctg ggcaaaaaca gtttgattgc
     4921 ggtaaccctg ttatcgataa atttgtacgc gctacgcgca tcgctaaaga aaagtgtgcg
     4981 taatagcgac tgtgcggcta aagcacttat tgacagacaa agtggtgaac tgattggcat
     5041 ctgtactttt acggcatatt cgctggaaag acaacgcgtt tctggcgtcc ttcagggttc
     5101 acaaccttca gaaattggtg ttgtcagatt agtcatgttg gggggagcgc ggaagtatca
     5161 aaagcggggc tttggtcagg acctactatg tgattttttt gaacatgtaa aaaaaattca
     5221 cccggcatta ccaattaaag gggtttatct tgatgctgac cctgccgcca ttaactttta
     5281 tgctcgtctc ggctttgttc agctttcagc gacaccaaat gcttttggtg ctgtacctat
     5341 gtttttggcg attcagcata ttctcgcggc ttagattatt atcgccaccc gccagggcaa
//
LOCUS       DADLCQ010000201         5350 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000201 DADLCQ010000000
VERSION     DADLCQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.204"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(75..557)
                     /locus_tag="JQG95_003877"
     CDS             complement(75..557)
                     /locus_tag="JQG95_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890267.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="JQG95_003878"
     CDS             complement(625..2574)
                     /locus_tag="JQG95_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY5890268.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="JQG95_003879"
     CDS             complement(2659..2994)
                     /locus_tag="JQG95_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY5890269.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="JQG95_003880"
     CDS             complement(2994..3350)
                     /locus_tag="JQG95_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890270.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="JQG95_003881"
     CDS             complement(3350..4846)
                     /locus_tag="JQG95_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890271.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="JQG95_003882"
     CDS             complement(4843..5004)
                     /locus_tag="JQG95_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY5890272.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="JQG95_003883"
     CDS             complement(5014..>5350)
                     /locus_tag="JQG95_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890273.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DADLCQ010000202         5337 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000202 DADLCQ010000000
VERSION     DADLCQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.205"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQG95_003884"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JQG95_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY5890274.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JQG95_003885"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JQG95_003885"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY5890275.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRSGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JQG95_003886"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JQG95_003886"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY5890276.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JQG95_003887"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JQG95_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY5890277.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1289 c         1456 g         1282 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgttcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLCQ010000203         5264 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000203 DADLCQ010000000
VERSION     DADLCQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.206"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(57..476)
                     /locus_tag="JQG95_003888"
     CDS             complement(57..476)
                     /locus_tag="JQG95_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890278.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILASSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            526..1704
                     /gene="ltrA"
                     /locus_tag="JQG95_003889"
     CDS             526..1704
                     /gene="ltrA"
                     /locus_tag="JQG95_003889"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY5890279.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFSFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1787..1966
                     /locus_tag="JQG95_003890"
                     /pseudo
     CDS             1787..1966
                     /locus_tag="JQG95_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1996..2478)
                     /locus_tag="JQG95_003891"
                     /pseudo
     CDS             complement(1996..2478)
                     /locus_tag="JQG95_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2734..3714)
                     /locus_tag="JQG95_003892"
     CDS             complement(2734..3714)
                     /locus_tag="JQG95_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY5890280.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3714..4913)
                     /locus_tag="JQG95_003893"
     CDS             complement(3714..4913)
                     /locus_tag="JQG95_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY5890281.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
BASE COUNT         1476 a         1187 c         1232 g         1369 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtggaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg aaagtaaact
      181 gtccgatgaa gcatcgaaat cttagatgat gccaaaatgg ggccgtacag ccgtcccctg
      241 ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat
      301 cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag
      361 gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg
      421 ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa
      481 aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca
      541 agggagcccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc
      601 aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg
      661 gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc
      721 gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc
      781 catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt
      841 gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac
      901 catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc
      961 atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta
     1021 ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg
     1081 ttccttcact atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag
     1141 aggtacgctg acgatattgt agtacattgt tcacgaatga gtgatgcaac aaggctaaag
     1201 aacaggttat cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat
     1261 atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa cgagtttctc ctttctcgga
     1321 tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg aacggtaccg aaaatgcatg
     1381 ccgggtgcgt caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata
     1441 catcgctcaa cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga
     1501 ggctggatcg agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt
     1561 gcaatgcagt cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag
     1621 gctcagcgaa agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat
     1681 ttactacgtg catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1741 tccgtgagca gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag
     1801 cgatgacgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
     1861 ggaaccagaa aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg
     1921 ggtacgtgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt
     1981 ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta
     2041 ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg
     2101 acgcatattc aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg
     2161 tatagttcca tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg
     2221 gcaccgctga taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt
     2281 cctgatttcc cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt
     2341 aggctgttgc accagccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg
     2401 tttgccatga gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt
     2461 gattttctgt tcagacatgg tatcccgctt acttttgttg ttaaagttta tccatcatgg
     2521 cgcaattgaa ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg
     2581 ctaagagaaa ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg
     2641 aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa
     2701 aactggtgag ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag
     2761 tacaagagca atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc
     2821 atatgagaac gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt
     2881 ccataacaaa gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc
     2941 cttggttata agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc
     3001 agtaataagc tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc
     3061 cagcctcagt cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga
     3121 ctgaacagga aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt
     3181 caccttagct gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc
     3241 tttgagacgt actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa
     3301 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
     3361 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
     3421 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt
     3481 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
     3541 cacaaaagtt tgagtatcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
     3601 tcggccagta ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag
     3661 gatatttgta ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag
     3721 ttggccctta taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata
     3781 cgtgcatttt tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg
     3841 gtatcaaacg actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc
     3901 atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc
     3961 gccagtttcg acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca
     4021 ataatctgta caagttcagg taaacgagcc aaatatttaa gcgtggaatg gaaatcaact
     4081 tgtgctggcg gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat
     4141 gcgtccagat gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca
     4201 ataatattct cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga
     4261 gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca
     4321 gggataactg acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac
     4381 atagcctgcg gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata
     4441 gtggctgatg actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg
     4501 tgaggatgag cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg
     4561 ccttttagac taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga
     4621 atgccacgat gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg
     4681 gttcctgctg ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca
     4741 tccacgcttg cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga
     4801 taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc
     4861 aacattttat ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct
     4921 ccgtgctatg taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg
     4981 caagtacata ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc
     5041 ggaatgaata ttactgtaag atagtatgcc tttccgggag aaagacgctg atttcccccg
     5101 aataatccag ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta
     5161 tttcgcggga aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg
     5221 ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga agga
//
LOCUS       DADLCQ010000204         5164 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000204 DADLCQ010000000
VERSION     DADLCQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.207"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..222
                     /locus_tag="JQG95_003894"
     CDS             <1..222
                     /locus_tag="JQG95_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890282.1"
                     /translation="SLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFH
                     WGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(394..723)
                     /locus_tag="JQG95_003895"
     CDS             complement(394..723)
                     /locus_tag="JQG95_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY5890283.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(728..913)
                     /locus_tag="JQG95_003896"
     CDS             complement(728..913)
                     /locus_tag="JQG95_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY5890284.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(910..3549)
                     /locus_tag="JQG95_003897"
     CDS             complement(910..3549)
                     /locus_tag="JQG95_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY5890285.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3757..4746
                     /gene="ldhA"
                     /locus_tag="JQG95_003898"
     CDS             3757..4746
                     /gene="ldhA"
                     /locus_tag="JQG95_003898"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY5890286.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4857..>5164
                     /gene="hslJ"
                     /locus_tag="JQG95_003899"
     CDS             4857..>5164
                     /gene="hslJ"
                     /locus_tag="JQG95_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY5890287.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1306 a         1322 c         1205 g         1331 t
ORIGIN      
        1 agtttgtacc gaatgtttgc cgataaaggt ttggtagtcg cgcaatatat tcgtaaccgt
       61 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
      121 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
      181 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgcatgt
      241 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
      301 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
      361 ctgtagcgtt atcccgatat tgcggttttg ctattaaaac tttcgcaccc atttaccgtt
      421 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
      481 cgcgatatca tctacagaca cattattttg ctttgcccgt tgttggtaac tcgctttacg
      541 ttcggcatta atttcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
      601 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaatgc
      661 cattacattg ctgcttacca gcccaacaag aaacgcacaa agaagtaatg ttttcttcat
      721 catcgcctca gaaaagatcg ctacgagttt caagcagctc ttcgacatct ttgtctgcct
      781 tgatgatgat ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg
      841 cagcgacttc aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca
      901 gtaaaatttt cattgttttt cctcacactc ttttccttgc ggacagtcat ttcccggcag
      961 acgtgcgttc tgctccaacc atgcctggag attatcgcca aagcgtaaac tgcgccacag
     1021 cgtaaaaata ttctcttcat gatggtaatt aaggtttacc gtaccgcttt taccatcaac
     1081 gcgactggta cctgtaatgt tggcctgcat ggttaataaa ccgagattat ctaagttaat
     1141 ttttgtcgat gaccggctaa tttccatata gcgcaaccag ttaattgctg aacccgcagt
     1201 catattgtct ttcaccaccg catctgcggt atctttatcc agccgcaagg tcattggccc
     1261 gctatttgcc agccaaccat ctttcactat ccatttttcg ttgtttagcc ataacggcaa
     1321 tgcaccacta aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc
     1381 gctggatgag agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg
     1441 tttcatcagc acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaagggttc
     1501 ttcttcagtc cacggataac gcccttgcaa atcagccgta atatttttcg ctgtcaccag
     1561 attaatcact tcggcaattc gcaacgtaac ggggccgcga gtccccagat gccaggctcc
     1621 atcggcaaaa cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca
     1681 ggcactaccg cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc
     1741 agaaaatgca acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg
     1801 caccagcggc tggaatacag tcagtgattg ttttggccac caggcgttac cgcgcagacg
     1861 aataccgtcc cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc
     1921 tttaaagagg aaataggtcg gattgcgccc atcgacgcta aattttaagg ttgatggcgg
     1981 taacacactg ccgccagtga acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa
     2041 ggagggatgc tgtgcgtcac gtacccagac gacgggcttg tcgagaatta atcgcggctt
     2101 ttctaccgtc atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt
     2161 aatcgtgctg tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt
     2221 aaaactccct tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt
     2281 cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat
     2341 ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg
     2401 gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta acgcgcctgg
     2461 ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata
     2521 taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt gactgttatc
     2581 catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg cttgcccctg
     2641 ggtcagcaca ttcagtcgtc cgctgaccag agcgttctca agccctgcct gccagttgtc
     2701 gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc tccagcgacc
     2761 atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc
     2821 gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca gctcggcatc
     2881 caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag
     2941 tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg gctgattttc
     3001 aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa
     3061 tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg cgagagagag
     3121 ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt
     3181 aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt gctccgtctg
     3241 cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca ataaccagcg
     3301 gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata
     3361 acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat
     3421 acgtgtcccg agcggtagcc agatgtccgc cagcgtggga acccacagcc cgagcgtcat
     3481 cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt
     3541 acccagcatg ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat
     3601 tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga
     3661 atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat atttttagta
     3721 gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca
     3781 aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa
     3841 ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta
     3901 tgtattttcg taaacgatga cggcagccac ccggtgctgg aagagctgaa aaagcacggc
     3961 gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa
     4021 gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac
     4081 gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt
     4141 gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc
     4201 gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg
     4261 cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg tgtggagtat
     4321 gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg cccgctgaca
     4381 ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg
     4441 atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa
     4501 aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga tctgttcttt
     4561 gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc tgcctgccac
     4621 aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct
     4681 cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg
     4741 gtttaatctt gcagctcccc tgcaatccag gggagctgat tcagataatc cccaatgacc
     4801 tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga accacaatga
     4861 agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta agtaatgaca
     4921 aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg
     4981 gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aaaatgatga
     5041 tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga
     5101 cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg
     5161 ataa
//
LOCUS       DADLCQ010000205         5149 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000205 DADLCQ010000000
VERSION     DADLCQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.208"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            188..445
                     /locus_tag="JQG95_003900"
                     /pseudo
     CDS             188..445
                     /locus_tag="JQG95_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(495..1445)
                     /gene="uspE"
                     /locus_tag="JQG95_003901"
     CDS             complement(495..1445)
                     /gene="uspE"
                     /locus_tag="JQG95_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY5890288.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTTMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1597..2349)
                     /locus_tag="JQG95_003902"
     CDS             complement(1597..2349)
                     /locus_tag="JQG95_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY5890289.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="JQG95_003903"
     CDS             complement(2544..3059)
                     /gene="ogt"
                     /locus_tag="JQG95_003903"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY5890290.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3070..3213)
                     /locus_tag="JQG95_003904"
                     /pseudo
     CDS             complement(3070..3213)
                     /locus_tag="JQG95_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3603..4418)
                     /locus_tag="JQG95_003905"
     CDS             complement(3603..4418)
                     /locus_tag="JQG95_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY5890291.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4515..>5149
                     /locus_tag="JQG95_003906"
     CDS             4515..>5149
                     /locus_tag="JQG95_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY5890292.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1173 c         1225 g         1376 t
ORIGIN      
        1 tattttaccg cgacaattca ttcagaacat caatagtcag ggaaggaagt agcaacatta
       61 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
      121 aacggtgaaa cgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
      181 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
      241 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgaccatcg gcggcgttat
      301 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga tttagtcatc
      361 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
      421 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
      481 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
      541 tcaggtttaa tgaccagcag gtcgcagcga agatgatcaa tcacctgttc cgccgtgttg
      601 ccgaggaatg ctgctgaaat accggtgcgt ccgaccgtgc ccagaaccac aattcccgcc
      661 tgtaaatgct ccgccaaatc aggaatcacc tcttcgggca gacctttttc tacgtgcgtc
      721 atgttttcat taatgccgaa tttctgccgc agggctttca ttgccagcaa atgttgccca
      781 cgaatggcat cgttataaac gctcgggtca aattccggca gttcaatcgc gatattaatt
      841 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
      901 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
      961 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
     1021 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
     1081 agacggtcat gttggtgcgc catttttagc accaaatcgt gtccgccgct gatcacttcc
     1141 tgaatgatgg cttcgaaagg acggttatgc cagaccactt taatttcaat gggaacgcca
     1201 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
     1261 tgacgcatag tggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
     1321 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
     1381 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
     1441 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
     1501 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
     1561 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
     1621 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
     1681 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
     1741 atagttacca atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
     1801 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
     1861 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
     1921 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacggaat
     1981 ttcacatacc atcgaggttt ccagcgcctg agcgaagctc gggtgatggc cgctgccgat
     2041 ggcgtcaaat cccactaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg
     2101 ctcagtgatt gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
     2161 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
     2221 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
     2281 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
     2341 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
     2401 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
     2461 tgtctatcta tttgaaactc cttaagttgt caagggtaac tttacataaa agtgtgaaca
     2521 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
     2581 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga
     2641 tggcaaggta cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc
     2701 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
     2761 atagtgcgta gtgttttcca gacttcgcgc tgaaatggcg tcccccccgt ggcagtggga
     2821 agcgtatcaa taatgctaag attaccggca aaatattcac gaagcttgtc gcttaaacca
     2881 cctgggttgg tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc
     2941 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
     3001 caaatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
     3061 cgttctctct taagacaaac gtgggtaaat acccggacct atcggcagac cgacaagata
     3121 ccacgccagc aacatcagca gccataccac caaaaagata agtggatagg gcaagactaa
     3181 cgaatagtaa gtacccagtt tcgcgtctgg tttgtcgcag tctgatagca taaagcgatt
     3241 ttttaatttt tctgcctgaa gaaatgaggg caagccatca catggatcga gggtgggata
     3301 attccagtca gtgaaatttt accgtaggct atctgttatt tgattctgtg gcgtgatagc
     3361 cgccagacac cactgaactc gtcactcatt ccccaatccc ggcgagaatg tggttattta
     3421 cagagctaaa aaatgaataa aaaaatagag cctgtcgaca tccgccagac tctacagtac
     3481 acacagcagt gcatcctcgt cttaaatccg gcctggggtc ctggtgaccg gtgacagtgt
     3541 tataacggtt ttacccatcc gttgccaatt ttcttcagtg acagatttca cgaaaatata
     3601 agttaaatca tttgtcttgt gattgcataa ttatgcatcg ttaatcgacg agtaaattta
     3661 ccgggcgagt agcccgttcg acgcttaaac atattgccga acgattcagc tgaggcatag
     3721 cccagacgat gggcaatttt ggtcaacgaa tatccatcat caagaagcgt cactgcagaa
     3781 ataacgtgca tttgctgaac ccagtggcga aatgttaatc ccgtctcttt aacaaataag
     3841 cgcatgagtg tacggctact gacgttaatt ttatccgcta gattatccag tgttggcaga
     3901 tacccttggt tgtttagcaa attctcacaa agctgctgta gccgctcatc atttggccaa
     3961 gggagttcaa gtgacatttt cacccccatt tttatttcct gaagtatcag tgtctcgagg
     4021 gctgaaaaac gtaactgttg tttatctgtg gtactttgcg acggtggaag ttggttaatt
     4081 gcaattagta attcccttaa caggggtgaa atacccgtca tgaagcaaac tgttcccagc
     4141 gtttcacttt ccgcttctga aaccagtacg gtttttaaaa gtacgttgcc tgtcgcaaca
     4201 aacttatgcg gcatttgtgg gggcagccaa agcgcgctgc tggcatggac tagccagacg
     4261 ctttctccag cccatacacg aacgactcct tcagccgggt aaagcaactg ggcatagaga
     4321 tgtttatgcc agggaactgt acctccattc agatgttccg aatccagtcc gataatgtga
     4381 tgccctggtg atgctttggg ttgcagtgta attgtcattt acagtaggta ttttgtcaga
     4441 aatcatcaaa taagccatgt taaattttaa atatgacgta tgcaagtttc cggatatata
     4501 cggtgtaaag agtgatgata atttctaaaa aacagcttat tggggtggta gcgatcggta
     4561 ttcttttgac tggcgttgtc ttttttatct ggagggtaag caaaggaaga tttatacaga
     4621 cgacggatga tgcttacatt ggtggtaata tcaccacggt ggcaagtaaa gtttctggtt
     4681 atatttctgc tatagaggtg cgcgataatc agagcgtaaa aaaaggcgat attatcctgc
     4741 gtctcgatga tcgggattat cgtgcaaatg tcgccaggct tgaggcaaaa ataaaaagta
     4801 gcaaagccaa tctcgaaggc attcaagcca ctattacgat gcagcagtcg ataattcaaa
     4861 gtgccagtga aacatggcag gcagtaaaac atgaggagca aaagcgcctg cgagatactg
     4921 aacgttatga aaaactcgca caatcagcgg caatatcgca acaaattatt gataacgcca
     4981 ggtttgacta ccagcaggtc gcagcaaagg agcgcaaagc cgcgaacgat ttcctggtgg
     5041 agaagcaacg gctggcagtt ttatctgctc aggaagaaaa cgtacgtgcg agtatcgaag
     5101 aagttcaggc cgctttgact caggcactac tggatctgga atataccct
//
LOCUS       DADLCQ010000206         5023 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000206 DADLCQ010000000
VERSION     DADLCQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.209"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            47..121
                     /gene="tnaC"
                     /locus_tag="JQG95_003907"
     CDS             47..121
                     /gene="tnaC"
                     /locus_tag="JQG95_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY5890293.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            342..1757
                     /gene="tnaA"
                     /locus_tag="JQG95_003908"
     CDS             342..1757
                     /gene="tnaA"
                     /locus_tag="JQG95_003908"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY5890294.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYRKEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1848..3095
                     /gene="tnaB"
                     /locus_tag="JQG95_003909"
     CDS             1848..3095
                     /gene="tnaB"
                     /locus_tag="JQG95_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY5890295.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3227..4402
                     /gene="mdtL"
                     /locus_tag="JQG95_003910"
     CDS             3227..4402
                     /gene="mdtL"
                     /locus_tag="JQG95_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY5890296.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4377..>5023
                     /gene="yidZ"
                     /locus_tag="JQG95_003911"
     CDS             4377..>5023
                     /gene="yidZ"
                     /locus_tag="JQG95_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY5890297.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1145 a         1209 c         1255 g         1414 t
ORIGIN      
        1 ctgagtgtaa taatgtagcg gcgtgtcttt cgaggataag tgcattatga atatcttaca
       61 tgtatgtgtg acctcaaaat ggttcaatat cgacaataag attgtcgatc accgccctta
      121 atttaccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt gatcgattag
      181 tttgctatat cttaattttg ccttttgcaa aggccatctc tcgtttattt acttatttta
      241 gtaaatgatg gtgcttgtat atatacatgg tgaattaatg gatattgcag atgtaatatt
      301 cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac tttaaacatc
      361 tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtactact cgcgcttatc
      421 gtaaagaagc aattattaaa tccggtatga acccgttcct gctggatagc gaagatgtgt
      481 ttatcgattt actgaccgac agcggcaccg gggcggtaac ccaaagtatg caggcagcga
      541 tgatgcgcgg cgacgaagcc tacagcggca gccgcagcta ctatgcgtta gccgagtcag
      601 tgaaaaatat ctttggttat caatatacta ttccgactca ccagggccgt ggcgcagagc
      661 aaatctatat tccggtactg attaaaaaac gcgagcagga aaaaggcctg gatcgcagca
      721 aaatggtggc attctctaac tatttctttg ataccacgca gggccatagc cagattaacg
      781 gctgtaccgt gcgtaacgtc tatatcaaag aagccttcga tacgggcgtg cgttacgact
      841 ttaaaggcaa ctttgacctc gaaggattag aacgcggtat tgaagaagtt ggcccgaata
      901 acgtgccgta tatcgttgca accatcacca gtaactctgc aggtggtcag ccggtttcac
      961 tggcaaactt aaaagcgatg tacagcatcg cgaagaaata cgatattccg gtggtaatgg
     1021 actccgcacg ctttgctgaa aacgcctatt tcatcaagca gcgtgaagca gaatacaaag
     1081 actggaccat cgagcagatc acccgcgaaa cctacaaata tgccgatatg ctggcgatgt
     1141 ccgccaagaa agatgcgatg gtgccgatgg gcggcttgct gtgcatgaaa gacgacagct
     1201 tctttgatgt gtacaccgag tgcagaaccc tttgcgtggt gcaggaaggc ttcccgacat
     1261 atggcggcct ggaaggcggc gcgatggagc gtctggcggt aggtctgtat gacggcatga
     1321 atctcgactg gctggcttat cgtatcgcgc aggtacagta tctggtcgat ggtctggaag
     1381 agattggcgt tgtctgccag caggcgggcg gtcacgcggc attcgttgat gccggtaaac
     1441 tgctgccgca tatcccggca gaccagttcc cggcacaggc gctggcgtgc gagctgtata
     1501 aagtcgccgg tatccgtgcg gtagaaattg gctctttcct gttaggccgc gatccgaaaa
     1561 ccggtaaaca actgccatgc ccggctgaac tgctgcgttt aaccattccg cgcgcaacat
     1621 atactcaaac acatatggac ttcattattg aagcctttaa acatgtaaaa gagaacgcgg
     1681 cgaatattaa aggattaacc tttacctacg aaccaaaagt attgcgtcac ttcaccgcaa
     1741 aactgaaaga agtttaatta atactacaga gtggctataa ggatgttagc cactctctta
     1801 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaag
     1861 ctgaaaaaaa gcactctgca ttttggggtg ttatggttat agcaggtaca gtaattggtg
     1921 gaggtatgtt tgctttacct gttgatcttg ccggtgcctg gtttttctgg ggtgccttta
     1981 tccttatcat tgcctggttt tcaatgcttc attccgggtt attgttatta gaagcaaatt
     2041 taaattatcc tgtcggctcc agttttaaca ccatcaccaa agatttaata ggtaacacct
     2101 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     2161 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     2221 atccacgtat tgtcgggatc tgcacagcca ttttcgttgc cagcgtattg tggataagtt
     2281 cgttagccgc cagtcgcatt acctcattgt tcctcgggct gaagattatc tcctttgtga
     2341 tcgtgtttgg ttctttcttc ttccaggtcg attactccat cctgcgcgat gccaccagca
     2401 ccactgcggg aacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2461 catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa cgcaaagata
     2521 agttaatcaa aagcgtggta tttggttcgc tgctggcgct ggtgatttat ctcttctggc
     2581 tctattgcac gatggggaat attccgcgcg aaagctttaa ggcgataatc tcctcaggcg
     2641 gcaacattga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc attatcgagt
     2701 tttgcctgct ggtgttctct aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2761 ggttgttcga ttatctggcg gacctgttta agattgataa ctcccacggc gggcgtttca
     2821 aaaccgtgct gttaaccttc ctgccacctg cgttgttgta tctgatcttc ccgaacggct
     2881 ttatttacgg gatcggcggt gccgggctgt gcgccaccat ctgggcggtc attattcccg
     2941 cagtgcttgc aatcaaagct cgcaagaagt ttcccaatca gatgttcacg gtctggggcg
     3001 gcaatcttat tccggcgatt gtcattctct ttggtataac cgtgattttg tgctggttcg
     3061 gcaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaagaagcc agccattcgc
     3121 tggcttcttg cctctcagga aatcacttat gtccaaatgg caactcgcct tatcctcctt
     3181 caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt cccgcttttt
     3241 gatttgtagt tttgccctgg ttttacttta tcccgccggg attgatatgt acctcgttgg
     3301 tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata ttgcgttctc
     3361 cgtatatctg gcggggatgg cagctgcgat gctatttgcc ggtaaagtgg ccgatcgttc
     3421 agggagaaag ccggtcgcca tacccggcgc ggcgctattt attattgcct cggtgttctg
     3481 ttcactggct gaaaccagca cgttatttct tgcaggccga tttctacagg ggttgggcgc
     3541 aggctgttgt tacgtggtgg cgttcgccat tttgcgcgac acgctggatg atcgacgtcg
     3601 ggctaaagtg ctgtcattac tcaacggtat tacctgcatc attccggtgt tagcgccagt
     3661 gctcggacat ctgattatgc ttaaattccc gtggcagagt ctgttctggg cgatggcaat
     3721 gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc gcccagcgtc
     3781 ccccgctgct tcggacaaac cacgagaaaa tagcgagtcg ctgcttaatc gttttttcct
     3841 cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcactttcg tcaatacatc
     3901 gccggtattg ctgatggaaa tcatggggtt tgagcgcgga gaatacgcca ccattatggc
     3961 gctgaccgct ggcgtcagca tgaccttttc attctcgacg ccatttgcgc tgggaatttt
     4021 taagccacgt acgttgatga tcacctcgca ggtgttattc cttgcggcgg ggatcaccct
     4081 tgccgtttca ccttcccatg cggtttctct gtttggtatc acgctgattt gcgccggttt
     4141 ctcggtaggt tttggcgtag cgatgagtca ggcgttagga ccattttcat tacgcgcggg
     4201 cgtagccagc tcgaccttag gtattgcgca ggtttgcggt tcgtcactgt ggatttggct
     4261 ggccgcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga ttgcctgtag
     4321 catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgccccgttg ccgctcatga
     4381 agaaatccat caccacgctt gatctcaatc tgctgctctg tctgcaactg ctgatgcagg
     4441 agcgcagcgt gaccaaagcg gcgaagcgga tgaacgtgac cccttcagcg gtgagtaagt
     4501 cgctggcaaa gttaagagcg tggtttgacg acccgctctt tgtgaactca ccgctggggc
     4561 tgtcgcccac acagctaatg gtcagcatgg agcaaaatct ggcggaatgg atgcaaatga
     4621 gcaatctgct gctggataaa ccgcaccacc agacaccgcg cggcctgaag tttgagttgg
     4681 cggcggaatc gccgctgatg atgatcatgc ttaatgcgct gtcgaaacgg atctaccaac
     4741 gttacccgca ggcgaccatc aaactacgta actgggatta cgattcctta gatgccatta
     4801 ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag ccatcctcgc tcgcgggagc
     4861 tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat gtaccctgcg
     4921 tctggttacg caaagatcat ccggcactgc atgaaacgtg gaatctggac acctttttac
     4981 gttatccgca tatcagcatt tgctgggaac agagcgacac ctg
//
LOCUS       DADLCQ010000207         5010 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000207 DADLCQ010000000
VERSION     DADLCQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.210"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..89)
                     /locus_tag="JQG95_003912"
     CDS             complement(<1..89)
                     /locus_tag="JQG95_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein hokD"
                     /protein_id="HAY5890298.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIAL"
     gene            complement(292..510)
                     /locus_tag="JQG95_003913"
     CDS             complement(292..510)
                     /locus_tag="JQG95_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890299.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(684..1019)
                     /locus_tag="JQG95_003914"
     CDS             complement(684..1019)
                     /locus_tag="JQG95_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890300.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
     gene            1127..1267
                     /locus_tag="JQG95_003915"
                     /pseudo
     CDS             1127..1267
                     /locus_tag="JQG95_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1267..2307
                     /locus_tag="JQG95_003916"
     CDS             1267..2307
                     /locus_tag="JQG95_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY5890301.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSKCDFCH"
     gene            complement(2335..2424)
                     /locus_tag="JQG95_003917"
     tRNA            complement(2335..2424)
                     /locus_tag="JQG95_003917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2388..2390),aa:Ser,seq:cga)
     gene            2518..3315
                     /gene="mtfA"
                     /locus_tag="JQG95_003918"
     CDS             2518..3315
                     /gene="mtfA"
                     /locus_tag="JQG95_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY5890302.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYVASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3416..3491
                     /locus_tag="JQG95_003919"
     tRNA            3416..3491
                     /locus_tag="JQG95_003919"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3449..3451,aa:Asn,seq:gtt)
     gene            3653..4915
                     /locus_tag="JQG95_003920"
                     /pseudo
     CDS             3653..4915
                     /locus_tag="JQG95_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1383 a         1179 c         1123 g         1325 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac tcgcgccatc
     1021 cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc gattaattgt
     1081 tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc agcgaaaatg
     1141 gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg
     1201 ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc
     1261 gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac
     1321 ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc
     1381 atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat
     1441 cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac
     1501 ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc
     1561 ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga
     1621 ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt
     1681 tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag
     1741 gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca
     1801 cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat
     1861 gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa
     1921 acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca
     1981 aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac
     2041 aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt
     2101 ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc
     2161 agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac
     2221 aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa
     2281 tattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga attttggcgg
     2341 agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg agaccggtcc
     2401 gttcagccgc tccggcatct ctccgttcag atggttgtca tgatgccagg aaatttggca
     2461 ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg
     2521 attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa
     2581 gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct
     2641 cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat
     2701 tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg
     2761 gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt ggtcgatgat
     2821 gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc
     2881 tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct
     2941 ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc
     3001 gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat
     3061 gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt
     3121 gatgcttatg ttgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt
     3181 agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt
     3241 tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc gttttcagcg
     3301 acgaatgttc attaattaac aactttgaag attaattaac caattgaaat ggcttatgaa
     3361 atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc
     3421 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     3481 cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca
     3541 acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc atcgggaaaa
     3601 attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc atatatccct
     3661 taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc
     3721 tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc tcaaataccg
     3781 tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct ccctgtctga
     3841 tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca
     3901 gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa acatggcgct
     3961 ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag
     4021 cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg
     4081 tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg
     4141 cacacggcag cacctgagta acataatgcg ccatgcggtc catcaggagt taatcgatac
     4201 gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc
     4261 cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg
     4321 tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact
     4381 gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac
     4441 gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca
     4501 tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg
     4561 taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac
     4621 ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg
     4681 cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt
     4741 agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc
     4801 agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg
     4861 ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc catagtatac
     4921 taacgaatcc cacactctgg caaattacca ccacctctac aggaggtggt tctatataga
     4981 cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DADLCQ010000208         4970 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000208 DADLCQ010000000
VERSION     DADLCQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.211"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(183..869)
                     /gene="kptA"
                     /locus_tag="JQG95_003921"
     CDS             complement(183..869)
                     /gene="kptA"
                     /locus_tag="JQG95_003921"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY5890303.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1017..1700
                     /locus_tag="JQG95_003922"
     CDS             1017..1700
                     /locus_tag="JQG95_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY5890304.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1697..2158
                     /locus_tag="JQG95_003923"
     CDS             1697..2158
                     /locus_tag="JQG95_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890305.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLSGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2171..3343
                     /gene="iadA"
                     /locus_tag="JQG95_003924"
     CDS             2171..3343
                     /gene="iadA"
                     /locus_tag="JQG95_003924"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY5890306.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3408..4319
                     /gene="hypT"
                     /locus_tag="JQG95_003925"
                     /gene_synonym="qseD"
     CDS             3408..4319
                     /gene="hypT"
                     /locus_tag="JQG95_003925"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY5890307.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGGDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEELL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="JQG95_003926"
     CDS             complement(4312..4704)
                     /gene="iraD"
                     /locus_tag="JQG95_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY5890308.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4701..4784)
                     /locus_tag="JQG95_003927"
     CDS             complement(4701..4784)
                     /locus_tag="JQG95_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY5890309.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1174 a         1304 c         1240 g         1252 t
ORIGIN      
        1 tgaccggaag cgacgctgat tccagtcccc aactgaccgc ctgccgtaaa tcactttaat
       61 ttcagcagtt gttattaaag gatacccttt tacgagggta tcccttcaga ctgataacaa
      121 acgctacatt gccggatgtg ctgcgcttac caggcctata taacccctgc aatatattga
      181 atttattgtt tttgtacccc agtcggcata aacgaccact caagaaactc aacagcaacc
      241 gttgatgtca gccagacccc gttttccgct tgccagaaag ggatgcctcg tttcgccatt
      301 tcctgtgctt tgacggttaa gacaaccggc gatccgtgtc ttgctcccac tttgcgtgcc
      361 gttgcttcat cagcagagag atgcacgtaa tgacgatccc ctgcaatcag cccctgtttt
      421 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
      481 tcggcaaagg aaatagcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
      541 ccatcactgg aataactaaa acgcttttta tcgctggttg cgaccacggt atccagcaat
      601 gcacgggtta atcgcttgcc cgctttttgg gcacaaagta tgagcttatt gatatccgcc
      661 catccttcac ggtccagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
      721 aaaaacttgc tcgtatccgc cagctctttt tcgttgtact ttgccatcgc gttttcgtcc
      781 ctggattttc tgtttttatt tactaatgtc attgtcactg ggtttatgaa ttttctgcaa
      841 ctcaatcctg cgcagaacct tttccacaca gcgtaatttt tcccctatgc aaaaaacgca
      901 tagccagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttcagttc
      961 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
     1021 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
     1081 atctggcttt ttttctcacc ataatctttt ttcccggtgt tttttccgga acagacagct
     1141 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
     1201 atggagccac cacctcattt cgcggggtgg gtggcgcggg ggctaaagat ggctttctct
     1261 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
     1321 gtcttggtgg attacgcgcc gcgcagcagt taatgacacc tgtgctaaaa ccgttgttgg
     1381 gcattccagg gatctgctcg ctggcactga tcgctaacct gcaaaacacg gatgccgccg
     1441 ccggtatgac caaagagctg gcgcaggaag gtgaaattac cgaacgcgat aaagtgattt
     1501 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
     1561 cggtgtttgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
     1621 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
     1681 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
     1741 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
     1801 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
     1861 tgaacccgta atggcgctgt ggggattatc cggtgaagcg gcaaccgtcc ttttagccgc
     1921 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcaaccg ctgacgcatt
     1981 aaccggccac gatgtcaccg ttttactgcc cgcaatgtat ttaatgggca acccggtgca
     2041 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
     2101 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
     2161 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
     2221 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
     2281 cgttgccagc aatatccctt ctgacattgt accgaactgc acggttgtcg atctcagcgg
     2341 gcagatcctc tgcccaggtt ttattgatca acacgtccat ttgattggcg gtggcggcga
     2401 agcaggtccc acgacgcgca cgccggaagt ggcgctaagt cgcctgacgg aagcgggcgt
     2461 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
     2521 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
     2581 tcatgtccct tcccgcacta ttacgggttc cgtggaaaaa gacgtggcga ttatcgatcg
     2641 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
     2701 cctggctaat atggcggcag aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
     2761 caccgtgttc cacatgggcg acagtaaaaa ggcgttaaag cctatttatg acctgctgga
     2821 aaactgcgat gtgccgatca gcaagctgct gccgacccac gttaaccgca acgtaccgtt
     2881 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
     2941 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
     3001 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
     3061 gaatttaacc catatcggtg ttgcaggttt tgaaacgttg ctggaaaccg tgcaggtgct
     3121 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
     3181 cggtttcctt aacatgagcg ggaaaggcga aattctgcca ggcaatgacg cagacttact
     3241 ggtcatgacg ccagaattgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
     3301 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
     3361 taatggacgt agaatgcccc caagggcggc tgacagagta aaacgtaatg gatgactgtg
     3421 gtgcggtttt gcataatatt gaaaccaaat ggctttatga ttttctgact ctggaaaaat
     3481 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
     3541 gcatccgtgc actggaacag gcgattggcg ttgaattgtt taaccgccag gtgacgccac
     3601 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
     3661 tagaaagcaa ccttgcagag ctgcgtggcg gcggcgatta cgcgcaacgt aaaatcaaga
     3721 tagccgctgc acactctctt tccctcggtc tgttaccgtc cattatcagc cagatgccgc
     3781 cgctctttac ctgggcaatt gaagccattg atgtcgatga agcggtcgat aaactgcgtg
     3841 aagggcaaag tgactgtatt ttttcctttc acgacgaaga gttgctggaa gcgccgtttg
     3901 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
     3961 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
     4021 acatggtgcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
     4081 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
     4141 cctggctgcc ggagtatgcc atccaacaag aaattcgcag cgggcagctc gttgtgctga
     4201 atcgggacga acaggtgatc ccgattcagg cctacgcata ccggatgaat acccgcatga
     4261 atccagttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg cttagctgac
     4321 attctcaagc gtcgcactgc gccaaccgcc attatcccag accagatcaa acgttagcgg
     4381 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc agtacgcttc
     4441 tttcaaatta acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
     4501 ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt gccactcggg
     4561 catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa gagtgaggag
     4621 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
     4681 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
     4741 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
     4801 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
     4861 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatctga taaaaatcat
     4921 caaactcaaa aaaaaaacgc tagtatattc tgaatattcc gaatagttac
//
LOCUS       DADLCQ010000209         4950 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000209 DADLCQ010000000
VERSION     DADLCQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.212"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            152..408
                     /locus_tag="JQG95_003928"
                     /pseudo
     CDS             152..408
                     /locus_tag="JQG95_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421719.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            551..772
                     /locus_tag="JQG95_003929"
                     /pseudo
     CDS             551..772
                     /locus_tag="JQG95_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(793..855)
                     /locus_tag="JQG95_003930"
                     /pseudo
     CDS             complement(793..855)
                     /locus_tag="JQG95_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1305..2717
                     /gene="ospC1"
                     /locus_tag="JQG95_003931"
     CDS             1305..2717
                     /gene="ospC1"
                     /locus_tag="JQG95_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY5890310.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2821..2970)
                     /locus_tag="JQG95_003932"
                     /pseudo
     CDS             complement(2821..2970)
                     /locus_tag="JQG95_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3046..4743
                     /gene="ospD3"
                     /locus_tag="JQG95_003933"
     CDS             3046..4743
                     /gene="ospD3"
                     /locus_tag="JQG95_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY5890311.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4860..>4950
                     /locus_tag="JQG95_003934"
     CDS             4860..>4950
                     /locus_tag="JQG95_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5890312.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGV"
BASE COUNT         1701 a          905 c          934 g         1410 t
ORIGIN      
        1 ttcaatcaaa gcgcagaact tctgcgtctt cttcgtcaaa aaaatcatct tcgtgaagct
       61 tcacgacata gcggggaagt ttgcttcttt gagaggcggg tttacgttta cgggatttag
      121 ctgaacgggc catataacca cctgaaagac aatgacattg cctgttttta taacggtaat
      181 tgcagaccat gacaaaccgc agccgtcagg ctgcctactc gagtcacaag gctctttgtg
      241 tccgatatgc cggcagcgca ttacccatga aactggctgg aacgtacacc ataaggtgaa
      301 gaaagttatg ggggcggtga agaattatct aaccttgtct tgttacatcc aaattgccat
      361 cgacagttac acagttgtga agccggctct ctcaaaaagg gcttataaag gcttgagcgg
      421 tgtaccggga aaccggtatg caccgttctt aggggagggc agtccagcaa tgaactgtcc
      481 ttacccgaca aacatccaaa acgaacggaa cgtgctggaa tccgcatata acccgttgta
      541 aagtgaggaa atggacacct cacttgctca tgagaacgcc cgcctgcggg cactgttgca
      601 gacgcaacag gacactatcc accagatggc tgaatacaac cgcctgctct cacagcggat
      661 ggcggcttat gcttccgaaa tcaaccgact gaaggcgctg gttgcgaaac tgcagcgtat
      721 gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga ttccattttc
      781 acgggcgatc tacgccacac aggctctggg ttgcagtgac tgctccatta aggcgatttt
      841 gaactcataa gggaagtaac aaaaaaactg caaaagtaca ataattatat atggccaaac
      901 taacctctat taaaaaacat cagtttttca cacttagttg agcttcatga aaaaatcatc
      961 gaggatttca ttgcagaata cacattccac taactggaag cactccataa gaggaatcag
     1021 agaaagtatc gtttttttat agtaaaattc attgctgttc aataaaattg atatagttat
     1081 taaattatga aaataatttc acaaaaaaga ttaacttgag ttcttaagca tcggatttta
     1141 gatattttga aatatctggc atcacataac atcaaaagta tataataccc ttatctaatt
     1201 acatgcagta tattgtatat atactcaaaa attgtaattc ataccctgct atatcttgta
     1261 gggaaagata ttaattaaaa ctgttttcat ataaggttca ttttatgaat atatcagaaa
     1321 cactgaactc agcaaatacc caatgcaata tagattctat ggataacaga ttacatacat
     1381 tgtttccaaa agtgacatca gtgcgaaacg ctgcacaaca aactatgcca gatgaaaaaa
     1441 atttaaaaga tagtgcaaat attattaaag atttctttag gaaaactata gcagcacaga
     1501 gttatagtag aatgttctct caaggctcta actttaaatc tttaaatata gcaattgatg
     1561 caccatcaga cgctaaagcc tcatttaagg ctattgagca ccttgacaga ttatcgaagc
     1621 attatatatc tgaaataagg gaaaaacttc atcctctttc tgcagaggaa ctcaatttgc
     1681 tttcgctaat tattaattct gatttaatct tcagacatca aagtaattct gatttgtctg
     1741 ataaaatttt aaacattaag tcattcaata aaattcagtc tgaaggaata tgcacaaaac
     1801 gaaacacata cgctgatgat ataaaaaaaa tagctaatca tgactttgtg ttttttggcg
     1861 ttgaaatctc taaccatcag aaaaaacacc ccctgaatac aaaacatcac actgttgatt
     1921 ttggtgcaaa tgcgtatatc attgatcatg actctccata tggatatatg acattaaccg
     1981 atcactttga taatgctatt ccacctgttt tttaccatga gcaccaatca tttttagata
     2041 aattttcaga ggttaataaa gaagttagtc gatacgtaca tggaagtaaa ggaattatag
     2101 atgtaccaat attcaatact aaagatatga agttagggct cggattatac ctgattgact
     2161 ttattagaaa aagtgaagac caaagcttca aggagttttg ctatggaaaa aatcttgccc
     2221 ctgtggatct ggatagaatc ataaactttg tttttcagcc agagtaccat atacctagga
     2281 tggtaagtac agaaaacttc aaaaaagtta agattagaga aatatcctta gaggaggctg
     2341 ttacagcatc taattacgaa gaaattaaca agcaggtcac taacaaaaaa attgctctcc
     2401 aggctctttt tctttcgatt actaatcaaa aagaggatgt cgccttatat atattatcta
     2461 attttgagat aactagacaa gatgttattt ccataaagca tgagttgtat gatattgagt
     2521 atctacttag cgctcataat tcaagctgta aagtacttga gtattttatc aataagggat
     2581 tggttgatgt aaacacaaag ttcaaaaaaa ctaatagtgg ggattgtatg ttggataacg
     2641 caataaaata tgagaatgca gaaatgataa aactattatt gaaatatggt gcaacatctg
     2701 acaataaata tatttaatca aaattgaata tcgtttagca aaaggcagta tttgcacaaa
     2761 atatcgatat attgtttatt gtcagtatgg ctcaatgtga taatagctgg aaagtttgat
     2821 gggtttcgcc ccgttgtagc ggtagtcgaa ctggaaggtc ttcaggcact gcttacagcg
     2881 atagagcagc ccccctgaac tggaatggcc gttccggata cccccctgag tttcagagta
     2941 acggtgacaa accacatcaa tctttgccat caatcatcca aagggcaaag agtacaacaa
     3001 cactaagtct gcgtcacaac ccatcaatga aaggaatata tacatatgcc atcagtaaat
     3061 ttaatcccat caaggaaaat atgtttgcaa aatatgataa ataaagacaa cgtctctgtt
     3121 gagacaatcc agtctctatt gcactcaaaa caattgccat atttttctga caagaggagt
     3181 tttttattaa atctaaattg ccaagttacc gatcactctg gaagacttat tgtctgtcga
     3241 catttagctt cctactggat agcacagttt aacaaaagta gtggtcacgt ggattatcat
     3301 cactttgctt ttccggatga aattaaaaat tatgtttcag tgagtgaaga agaaaaggct
     3361 attaatgtgc ctgctattat ttattttgtt gaaaacggtt catggggaga tattattttt
     3421 tatattttca atgaaatgat ttttcattcc gaaaaaagca gagcactaga aataagtaca
     3481 tcaaatcaca atatggcatt aggcttgaag attaaagaaa ctaaaaatgg gggggatttt
     3541 gtcattcagc tttatgatcc caaccataca gcaactcatt tacgagcaga gtttaacaaa
     3601 tttaacttag ctaaaataaa aaaactgact gtagataatt ttcttgatga aaaacatcag
     3661 aaatgttatg gtcttatatc cgacggtatg tctatatttg tggacagaca tactccaaca
     3721 agcatgtcct ccataatcag atggcctgat aatttacttc accccaaagt tatttatcac
     3781 gcgatgcgta tgggattgac tgagctaatc caaaaagtaa caagagtcgt acaactatct
     3841 gacctttcag acaatacgtt agaattactt ttggcagcca aaaatgacga tggtttgtca
     3901 ggattgcttt tagctttaca aaatgggcat tcagatacaa tcttagcata cggagaactc
     3961 ttggaaactt ctggacttaa ccttgataaa acggtagaac tactaactgc ggaaggaatg
     4021 ggaggacgaa tatcgggttt atcccaagca cttcaaaatg ggcatgcaga aactatcaaa
     4081 acatacggaa ggcttctcaa gaagagagca ataaatatcg aatacaataa gctgaaaaat
     4141 ttgctgaccg cttattatta tgatgaagta cacagacaga tacccggact aatgtttgct
     4201 cttcaaaatg gacatgcaga tgctatacgc gcatacggtg agctcattct tagcccccct
     4261 ctcctcaact cagaggatat tgtaaatttg ctggcctcaa ggagatatga caatgttccc
     4321 ggacttctgt tagcattgaa taatggacag gctgatgcaa tcttagctta tggtgatatc
     4381 ttgaatgagg caaaacttaa cttggataaa aaagcagagc tgttagaagc gaaagattct
     4441 aatggtttat ctggattgtt tgtagccttg cataatggat gtgtagaaac aattattgct
     4501 tatgggaaaa tacttcacac tgcagacctt actccacatc aggcatcaaa attactggca
     4561 gcagaaggcc caaatggggt atctggatta attatagctt ttcaaaatag gaattttgag
     4621 gcaataaaaa cttatatggg aataataaaa aatgaaaata ttacacctga agaaatagca
     4681 gaacacttgg acaaaaaaaa tggaagtgat tttctagaaa ttatgaagaa tataaaaagc
     4741 tgaatattat ctcgtggtga tacactgaat ttgaggtaac cggctcattt aaaccgtctg
     4801 gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga cactatcgta
     4861 tggaataccg gacctggatc actgaagctt tacgccttca tttcgaagaa catttaccac
     4921 gggttgtggc cggacgtcgc ctgggcgtac
//
LOCUS       DADLCQ010000210         4882 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000210 DADLCQ010000000
VERSION     DADLCQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.213"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..234
                     /locus_tag="JQG95_003935"
     CDS             <1..234
                     /locus_tag="JQG95_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system protein TorT"
                     /protein_id="HAY5890313.1"
                     /translation="KRGRVIMAASDQMVWQGELAVEQAIRQLQGQSVSDNVSPPILVL
                     TPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(207..899)
                     /gene="torR"
                     /locus_tag="JQG95_003936"
     CDS             complement(207..899)
                     /gene="torR"
                     /locus_tag="JQG95_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY5890314.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            986..2158
                     /gene="torC"
                     /locus_tag="JQG95_003937"
     CDS             986..2158
                     /gene="torC"
                     /locus_tag="JQG95_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY5890315.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            2158..4698
                     /gene="torA"
                     /locus_tag="JQG95_003938"
     CDS             2158..4698
                     /gene="torA"
                     /locus_tag="JQG95_003938"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAY5890316.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRAMVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIPIA
                     RFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVIAI
                     DNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDIFR
                     ELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFDHP
                     QMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGGPG
                     SQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDVVR
                     VFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIGTS
                     QLAQAASAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            4695..>4882
                     /gene="torD"
                     /locus_tag="JQG95_003939"
     CDS             4695..>4882
                     /gene="torD"
                     /locus_tag="JQG95_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY5890317.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         1188 a         1248 c         1375 g         1071 t
ORIGIN      
        1 aagcggggaa gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggca
       61 gttgagcagg ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg
      121 attttagttc tgacgccgaa aaatgccgac cgagaacata tccgccgctc gctgtcaccg
      181 gggggatttc gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca
      241 ccatgttgcg tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca
      301 tcgacggtgc gcaggtcagg gttttccacc cgacgcgcag aaagcatacg cagcagacgc
      361 tcacggctga gaatttcgcc cggattagtc acaaatgcca ccaacatttc atactctgcg
      421 cgggtcagtt taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa
      481 taacccgcaa agcgatagca gtagtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg
      541 attcgccaga gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg
      601 acgtaatcgt ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg
      661 gtaaccagaa taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca
      721 ttttcatcgg gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt
      781 tcccgtagcc cggcaccgct cgccgtaacg gaaacggtat acccctcctg agtgaagtag
      841 gattgtaatc gcgcctgggt aaccggctta tcttcaacaa taacaatgtg atgtggcatc
      901 agagggtttt actcattctg ttcatatctg ttcatatttg ctcattaaga tcgcttcact
      961 aaaccataat tctacagggg ttattatgcg gaaactctgg aacgcgctac gccgacccag
     1021 tgctcgttgg tcggtactgg cgctggtcgc aattgggatt gtgattggca ttgcgctgat
     1081 tgtattgcca cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca
     1141 cagtatgcaa ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg
     1201 cgtgcgagct gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg
     1261 caaactggaa gcgagcaacg acatttacca gacctttatt gcccactcca ttgatacacc
     1321 tgaaaaattc gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga
     1381 aaacaactcg gcaacctgtc gctcctgcca taactacgat gcgatggatc atgccaggca
     1441 gcatcctgaa gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga
     1501 ctgtcataaa ggtattgccc accagttacc ggatatgagt agcggcttcc gtaagcagtt
     1561 cgatgagctg cgcgccagtg ctaatgacag tggtgacacg ctgtactcta ttgatattaa
     1621 gccaatttat gcggcgaaag gcgataaaga agcctctggt tctctgctgc ctgcttcgga
     1681 agtgaaagtc cttaaacgtg acggcgactg gctgcaaatt gaaattaccg gctggacgga
     1741 aagcgccgga cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc
     1801 gattcgtggt gatgtgcagc agcaggtgaa aacgctggag aaaaccaccg ttgccgacac
     1861 caataccgag tggagcaagt tgcaggccac cgcgtggatg aagaaaggcg acatggtgaa
     1921 cgatatcaaa ccgatctggg cctatgcgga ttcgttgtac aacggcacct gtaaccagtg
     1981 ccacggcgca ccggaaatcg cccactttga cgctaacggt tggatcggca cgctcaacgg
     2041 catgattggc tttaccagtc tcgataaacg tgaagaacgc actttgttga aatatctgca
     2101 aatgaatgcg tctgacaccg caggtaaggc tcacggcgat aagaaggaag aaaaataatg
     2161 aacaataacg atctctttca ggcatcacgt cggcgttttc tggcacaact tggcggctta
     2221 accgtcgccg ggatgctggg gccgtcattg ttaacgccgc gccgtgcaac tgcggcgcaa
     2281 gcggcgactg aggctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct
     2341 atccgcgcga tggtgaagga tggtcgcttt gtggcggcaa aaccgttcga actggataaa
     2401 tatccgtcga aaatgatcgc cggattgccg gatcacgtac acaacgcggc acgtattcgt
     2461 tatccgatgg tacgcgtgga ctggctgcgt aagcgccatc tgagcgacac ctcccagcgc
     2521 ggtgataacc gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa
     2581 ctggaacgcg tacagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa
     2641 tcgacgggga tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt
     2701 aatagcgttg gtacgggcgg agattactct accggtgctg cgcaggtgat cctgccgcgc
     2761 gtagtcggtt caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac
     2821 agcaaaacca ttgtgctgtg gggctccgat ttgctgaaaa accagcaagc gaactggtgg
     2881 tgcccggatc acgatgttta tgaatattac gagcagctaa aagcgaaagt cgccgccggt
     2941 gaagttgagg tcatcagcat cgatccggtt gtcacatcca cccatgagta tctggggcgc
     3001 gagcatgtga agcacattgc ggttaacccg caaactgacg tgccgctgca actggcgctg
     3061 gcacatacgc tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg
     3121 ggttttgagc agttcctgcc gtatctgctg ggtgagaaag acggtcagcc gaaagatgcc
     3181 gcatgggctg aaaaactgac cggcattgat gccgaaacca ttcgtgggct ggcgcggcag
     3241 atggcggcga acagaacgca aattattgct ggctggtgcg tgcagcgtat gcagcacggt
     3301 gaacagtggg cgtggatgat tgtggttctg gcggcgatgc tggggcaaat tggcctgcca
     3361 ggtggtggtt ttggctggca ctacaacggc gcaggcacgc cggggcgtaa aggcgttatt
     3421 ctgagtggtt tctccggctc tacgtcgatt ccgcctgttc acgacaacag tgactacaaa
     3481 ggctacagca gcactattcc gattgcccgt tttatcgatg cgatcctcga accggggaaa
     3541 gtgatcaact ggaacggtaa atcggtaaaa ctgccgccgc tgaaaatgtg tatttttgcc
     3601 ggaactaacc cattccatcg ccatcagcag atcaaccgca ttattgaagg ctggcgcaag
     3661 ctggaaacgg ttatcgccat agataaccag tggacctcaa cctgccgctt tgccgatatc
     3721 gtgctgcctg cgaccacgca gtttgagcgt aacgatctcg accagtacgg caaccactcc
     3781 aaccgtggca ttatcgccat gaaacaggtg gtgccgccgc agttcgaggc gcgcaacgac
     3841 tttgatattt tccgcgagct gtgccgtcgc tttaatcgtg aagaagcctt taccgaaggg
     3901 ctggacgaaa tgggctggct gaaacgcatc tggcaggaag gtgtacaaca aggcaaagga
     3961 cgcggcgttc atctgccagc gtttgatgac ttctggaata acaaagagta cgtcgagttt
     4021 gaccatccgc agatgtttgt tcgccaccag gcattccgcg aagatccgga tctcgaaccg
     4081 ctgggcacgc cgagtggcct gattgagatc tactcgaaaa ccatcgccga tatgaactac
     4141 gacgattgtc aggggcatcc gatgtggttt gagaaaatcg aacgctccca cggtgggccc
     4201 ggctcgcaga agtatccgtt gcatctgcaa tctgtgcatc cggatttccg acttcactcg
     4261 cagctatgtg agtcggaaac gctgcgtcag caatatacgg tagcgggtaa agagccagtg
     4321 ttcattaacc cgcaggatgc cagcgcgcgc ggtattcgta acggtgatgt ggtacgcgtc
     4381 tttaacgctc gcggtcaggt gttggcagga gcagtagttt ctgaccgcta tgcacccggc
     4441 gtggcgcgaa ttcacgaagg ggcatggtac gatccagata aaggcggcga gcctggtgcg
     4501 ctgtgcaaat acggtaaccc caacgtgctg accatcgaca tcgggacttc gcagctggcg
     4561 caggcggcca gtgcgcacac tacgctggtg gaaattgaga agtacaatgg aacagtggag
     4621 caggtgacgg cgtttaacgg ccccgtggag atggtggcgc agtgcgaata tgttcccgcg
     4681 tcgcaggtga aatcatgacc acgctgacag cacaacagat tgcctgtgtt tacgcctggc
     4741 tggcgcagtt gttctcccgt gaactggacg atgaacaact gacgcaaatc gccagtgcgc
     4801 agatggctga atggttttcg ttgctgaaaa gcgaaccgcc gctcactgcg gcggtgaatg
     4861 atctggaaaa cagtattgcc ac
//
LOCUS       DADLCQ010000211         4867 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000211 DADLCQ010000000
VERSION     DADLCQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.214"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..969
                     /locus_tag="JQG95_003940"
     CDS             <1..969
                     /locus_tag="JQG95_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY5890318.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1550..2773
                     /locus_tag="JQG95_003941"
     CDS             1550..2773
                     /locus_tag="JQG95_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890319.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            3020..3586
                     /gene="slp"
                     /locus_tag="JQG95_003942"
     CDS             3020..3586
                     /gene="slp"
                     /locus_tag="JQG95_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY5890320.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            3742..4272
                     /locus_tag="JQG95_003943"
     CDS             3742..4272
                     /locus_tag="JQG95_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5890321.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4314..4867)
                     /locus_tag="JQG95_003944"
                     /pseudo
     CDS             complement(4314..4867)
                     /locus_tag="JQG95_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
BASE COUNT         1557 a          998 c          905 g         1407 t
ORIGIN      
        1 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
       61 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      121 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      181 tgctcgacaa tacccatgtt acgtggattg ttgtcagcga aagccgggtt tggtccgact
      241 ctcacttttt tatttgtttc accattactt aatcccatta tcgtcggact aatgtggatg
      301 acctttggtt ggaaagtcac cttgttgtac gcgattatcg cagccggcgt ctccgttctt
      361 gccagtatta tcctggattc tctggggttt gaacgtcata tcattgccag taaaagctca
      421 tcagcaaatt gttgtgctcc agcaaaaact tcgccgggaa cgacatatac gcccataaaa
      481 gtgagttgtt gtagtccagc ggctaaagcc attgagaacc ccgtagttac ctgttgcagt
      541 accaaagctg tagtaagtat taatcccata aaactagcca ccaaagatgc gttgcaacag
      601 tttaaagatg tactgccata tcttttgtta ggagtattaa taggatcttt tatttatggc
      661 tttattccct cagcgtggat tgccgctcat gcaggagcag ataatccctt cgccatccca
      721 ttaagcgccg ttgtcggtat tccgctgtat atccgggctg aggcggttat ccctctggca
      781 tctgttctga tgacaaaagg aatgggtctg ggagcattaa tggcattaat catcggcagt
      841 gccggcgcaa gcctgacgga agtgatatta cttaaatcaa tgttcagaat gccgatgata
      901 gctgcattcc tgacggttat attaggtatg gctatcttga tgggctattt gactcaattg
      961 ctattttaaa tagatgccag cagtaaacta taagatgcat agttagtgcc atatccttct
     1021 aagtagaaaa tcatagacag gataatcctt aaaaatcaca aatgatttaa ctaatgcatt
     1081 gagaatgatg acggctcggt caacaagtca tcaacagaca ataaccacgt aatcatgctt
     1141 ctctgacatt ttatcggaga agcaatgact ctttgtctca gtaccctcct cctttttaaa
     1201 aggcagattt cagtcgctaa tacaacacat ttttaaatac actttaacct ggttcattat
     1261 tacacatcta aaacatcaca aaaatattac ttcacacact atcggagaac ataataactt
     1321 tttaaaatga gcgttttttg atagttgtta acgttttcct ttacgatact atcactgccc
     1381 tttttttata cacaaatatt accgggtgga aatgaaaggg aatttaaagg taattatatc
     1441 aggaagatat aacgcattat atttattgtg tgtagaagaa aatccattcc tgttctaatt
     1501 atccaattta aaaacattag cataaaacag atgatgaata aggaatgtta tgtcaattga
     1561 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
     1621 cgataatgtt aaaacgccag acacaccgaa gtggccttgt gataattggg aagaacaaca
     1681 acccattaat tccacattct ctggagaagg atacaaccct gaacaatttg atttagcgca
     1741 acaacaactc caacaaatta atgcatgcca cacgaacacc acatatacaa atgcagacta
     1801 ctcaaaagtt gtggttcaac ttgtaagtct tattaataac attgaaacga taagttcgac
     1861 accacttacg caacagacac aatcaatact taatcagatt cataacatta gatacgaaaa
     1921 ggataaggat tctgtatgtc aaatcattgt tgtcgttaat ccagaaccca acaatccaat
     1981 cataactaaa attctcgtag aagaaggaat acccgaaaga ttcagtgtcc aaaccgtttc
     2041 ttccgataat aaaaattttg ctgggcaacg agctgactta ccaactgata taagagacat
     2101 tcaaagcttg tacttaaaaa tggcgaagct gtatattgag cacagtaaaa atgaaaatag
     2161 gatggaagcc cttgctggat gcgactttat tgatttcaac atgacagggc aagatatgtc
     2221 caagctagtc ctaaccttat caaaatttta ttttgaagat ctacttaata taaatttcac
     2281 tgacgcaaat ttatttaaca ctattttttc acacaaggaa aatcccatcc ccaaactaca
     2341 taaatatgaa caacatcttg acaaacaaat caatggtttg ttttctacat tgttaactat
     2401 taatgacaac tctttacgcg cgaaagcgga aattgcttcg agaataattg atttcttaga
     2461 ggcaaaggta gtgaatcttt catttgatga catattaaaa tataagcaag agttcaaaaa
     2521 aatatgttat aaacaacttc aagagtttac aacaccctct ctatacaata aaattcaaaa
     2581 atgggcaaca atgtctaaaa atgaattcat agcatttcat tacgagacac ttcaacccga
     2641 aaagatttca tacccctttt atttaaagag agatctgcct aatgaaaaag atataaatta
     2701 tggggtggaa atagaaatac catctggcaa aagaattcgc ttatcaaatc attatcagaa
     2761 tataatacca taatattgat tattagcact ttataatcat tgaataaaaa tagattttat
     2821 gtaattttaa aacaaagggt tatattattg ggcgatggat actcatacca cgccccaaaa
     2881 taacggaatt gaactaaaca gaagggatat aaacatcaga caggtttacg ttactatcag
     2941 gcatatcacc tcagaatcag atgaaaacta taaagaaata tctattatgg ttttaatatt
     3001 tgttgataag gatagtaaca tgaacatgac aaaaggtgca ctcatcctca gcctttcatt
     3061 tttgcttgcc gcatgtagtt caattccgca aaatatcaaa ggcaataacc aacctgatat
     3121 tcaaaaaagt tttgttgctg ttcataacca gccggggtta tatgttggtc aacaagcgcg
     3181 ctttggtggg aaggttatca acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc
     3241 tgtattaccg ttggatagct atgcgaagcc tgatattgaa gccaactatc agggccgact
     3301 gctcgccaga caaagcggct tccttgatcc agtgaactat cgtaatcact ttgttaccat
     3361 cctcggtacc attcagggtg aacaaccagg ctttatcaat aaagtcccgt ataacttcct
     3421 ggaagtgaat atgcagggca tccaggtgtg gcatttgaga gaagtggtta ataccaccta
     3481 taacctgtgg gattacggct atggtgcatt ctggccggaa ccgggctggg gtgcgcctta
     3541 ctacaccaat gcggtgagtc aggtaacacc tgagctggtc aaataacacc accgaaagat
     3601 gcagatgctt tctcagcatc tgcatcatgt attacataaa attaatatac agtaagctaa
     3661 ctattattat tataagccct gtcctgttaa ttacctttgg caaactgatt ataaagttaa
     3721 tgtccgcacc aggagtcggt tatgtttctt ataattacca gggatacgat gttcttcacc
     3781 gcgatgaaaa acattctgag taaaggtaat gtcgttcata tacagaacga agaagagatc
     3841 gacgtaatgt tgcatcagaa tgccttcgtc attattgata cattaatgaa taatgtattt
     3901 cattctaatt ttctcactca aattgaacga ttaaaacctg tccatgtcat tgttttctcc
     3961 ccctttaata ttaaacgctg tctggggaaa gtaccggtga cctttgttcc gcggactatc
     4021 actatcattg attttgtcgc actcatcaat ggcagttact gctctgtgcc tgaagcgaat
     4081 gtgtcacttt cgcgcaagca acatcaggtt ctgagctgca ttgcgaatca aatgacaact
     4141 gaagatattc tggagaaact gaaaatatcg ctaaaaacgt tctactgcca taaacacaat
     4201 atcatgatga tcctcaatct taagcggatc aatgagctgg tacgccatca gcatattgat
     4261 tatctggtgt gaatttcagg cttacggtga gtctggctac gctgccacac agattagcta
     4321 attgaaacgc ctttcacccc tgccatacct tttaataatc gcaacaggtc ttctatcaac
     4381 gttgtggcat gaagctggat atcaatggcg accacttcat gatcttcatt ttcctgcaac
     4441 gaaactaaat cagtttttat tttttgctgc ttaaaccagt cgagcattga taccgtatta
     4501 ccgttcacta atgtcagctg taaatggtaa tttttgttca tcaggcggaa ggttaattga
     4561 tggaagactt ccagcaccaa caaggtcatc actgaaccat aaatacccag ttcgtacata
     4621 ccgctgccaa taaccatacc tatgcggcgg tcacccagat atccgctgcc gtcgtcagac
     4681 ctacaatatt ttggttacga acgagaatgt tacctgcacc gataaacccg acgcccgtca
     4741 ccacctgagc agcaatacgg ctggggtcga gtccgacgtg atctaaagac agcacgtcag
     4801 caaaaccata tttcgaaaca atcataaaca gggcgcttcc catgccaatt aatacatgtg
     4861 tgcgtaa
//
LOCUS       DADLCQ010000212         4862 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000212 DADLCQ010000000
VERSION     DADLCQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.215"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..425
                     /locus_tag="JQG95_003945"
     CDS             <1..425
                     /locus_tag="JQG95_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5890322.1"
                     /translation="PRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLG
                     VYCSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG
                     TVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            759..3038
                     /gene="ydeP"
                     /locus_tag="JQG95_003946"
     CDS             759..3038
                     /gene="ydeP"
                     /locus_tag="JQG95_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY5890323.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIEHDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3286..3489
                     /gene="safA"
                     /locus_tag="JQG95_003947"
     CDS             3286..3489
                     /gene="safA"
                     /locus_tag="JQG95_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY5890324.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3557..4318
                     /gene="ydeO"
                     /locus_tag="JQG95_003948"
     CDS             3557..4318
                     /gene="ydeO"
                     /locus_tag="JQG95_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY5890325.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1390 a         1038 c         1078 g         1356 t
ORIGIN      
        1 atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg ccaacaggcg
       61 gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct ggaagcgcag
      121 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
      181 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
      241 cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
      301 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
      361 caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
      421 aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc gcggataatt
      481 attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt gaagccagag
      541 tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg atgcttaact
      601 tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag agtagtccct
      661 tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat catcgctatt
      721 acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa attgaatcct
      781 accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca gtacgtaagc
      841 agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca gagggctttg
      901 actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac atttgtgaaa
      961 acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc tctttctttg
     1021 ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag gctgcggggc
     1081 gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca ttaagctggc
     1141 aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc aatcaggttg
     1201 aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag ctttttgccc
     1261 gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa ccgacaagcg
     1321 tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa gactttgaga
     1381 agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct cgcatgctga
     1441 cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat cctctacagg
     1501 aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg acgaactctg
     1561 agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg gcgttgctca
     1621 aggggatgat gcgcctgtta attgagcacg atgatgctgc aagtgccgca ggtcggccct
     1681 cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag ctacgccgtg
     1741 acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt cagacacaaa
     1801 tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt tacggaatgg
     1861 ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat ttgctgttga
     1921 tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt cactctaatg
     1981 tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt ctggctcgcc
     2041 tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca attgccagca
     2101 tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc aactttgcac
     2161 tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat ttggcggtac
     2221 acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc tatattctgc
     2281 cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg gtaaccgttg
     2341 aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc ggtgtaatgc
     2401 tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc cagagcgtgg
     2461 tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt gaagctgtgc
     2521 tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt cacctgataa
     2581 atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc attaccagca
     2641 aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg gcgacagtac
     2701 gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat cgaggggtat
     2761 tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc cgtgtaaaaa
     2821 acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc tcacgccgca
     2881 tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg acctattttc
     2941 cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggc attcctggct
     3001 ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca tttcttctgc
     3061 tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt gctcacatgg
     3121 attaaattaa actacatact ataagatata aacttctgcc tacagctgta agaaactccg
     3181 ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata ttaagcatac
     3241 tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca tgcgaccaca
     3301 gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgc aattgtggtt
     3361 gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca gctgagtatt
     3421 tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag tttgcaagac
     3481 tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata aaactttatt
     3541 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgctttcaa
     3601 cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga tggtagataa
     3661 cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
     3721 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
     3781 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
     3841 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
     3901 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg tgctatccgt
     3961 ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga agctgaaaga
     4021 tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa agcaagagca
     4081 aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa atttgatacg
     4141 cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta catcttattt
     4201 tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta aggagtaccg
     4261 ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag ctatttgatt
     4321 atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg cagccattcc
     4381 ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac atccatcaca
     4441 tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc ggcattttgt
     4501 cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg cgagaaatta
     4561 cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct ttttttagct
     4621 aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc tcttcgtaaa
     4681 attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc attaaagaaa
     4741 agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc atttgctgct
     4801 catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt ctcagacttt
     4861 ac
//
LOCUS       DADLCQ010000213         4824 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000213 DADLCQ010000000
VERSION     DADLCQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.216"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="JQG95_003949"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="JQG95_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY5890326.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="JQG95_003950"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="JQG95_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY5890327.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="JQG95_003951"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="JQG95_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY5890328.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3692)
                     /gene="sitA"
                     /locus_tag="JQG95_003952"
     CDS             complement(2802..3692)
                     /gene="sitA"
                     /locus_tag="JQG95_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY5890329.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            complement(4189..>4824)
                     /locus_tag="JQG95_003953"
     CDS             complement(4189..>4824)
                     /locus_tag="JQG95_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5890330.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1287 a         1175 c         1142 g         1220 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atatcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa cgcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaacgctgta taaggggcaa
     3961 aaaaggggca aaaaaggggc aaaaaagagt gggttatcgt agcttattgt tgtcgctgat
     4021 aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt gtgatttttg
     4081 cccttacttg ttcaggtcgt attgttcttt ctttgtaatt tgttgatttt cttgcattat
     4141 ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact acgataaccg
     4201 cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac cgtaaatttc
     4261 atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg ggttcgctcc
     4321 tgccgtcaac agccagcagg caaaagtatg tcgcgtgtgg tacggattac ggcggcgaat
     4381 accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg agtaataagg
     4441 tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt gtttttcagt
     4501 tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc cggtcagttt
     4561 gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gttaccgtcc ggatcccggc
     4621 atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga cattcacaat
     4681 tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt gacgaagccc
     4741 ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt ccattaatgc
     4801 attgaattct gttttagata acgg
//
LOCUS       DADLCQ010000214         4801 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000214 DADLCQ010000000
VERSION     DADLCQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.217"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..527
                     /locus_tag="JQG95_003954"
     CDS             <1..527
                     /locus_tag="JQG95_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY5890331.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="JQG95_003955"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="JQG95_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY5890332.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3206)
                     /gene="gap"
                     /locus_tag="JQG95_003956"
     CDS             complement(2205..3206)
                     /gene="gap"
                     /locus_tag="JQG95_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY5890333.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3395..3925
                     /gene="cybB"
                     /locus_tag="JQG95_003957"
     CDS             3395..3925
                     /gene="cybB"
                     /locus_tag="JQG95_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY5890334.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4170..4343
                     /locus_tag="JQG95_003958"
     CDS             4170..4343
                     /locus_tag="JQG95_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890335.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="JQG95_003959"
     CDS             complement(4415..4564)
                     /gene="hokB"
                     /locus_tag="JQG95_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY5890336.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4665..>4801)
                     /locus_tag="JQG95_003960"
     CDS             complement(4665..>4801)
                     /locus_tag="JQG95_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5890337.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT         1226 a         1103 c         1198 g         1274 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 tttccgagaa tggacaccag ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca
     2521 tgacctttca gtttgccgct cagttccggg atcaccagac caatggcttt tgccgccccc
     2581 gtagtgtggg gaatgatatt ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc
     2641 gggccatcca ccagtgactg ggtgccagta taggcatgaa tggtcgtcat cgtgccgact
     2701 tctatcccga aactgtcatg caaggctttg gccatcggcg caaggcagtt agtggtgcat
     2761 gacgccacgg aaacaatggt gtcgttgcca tccagagtgt cgtcattaac gttataaacg
     2821 atggttttca tttcaccggc aggagcggaa atcaacacct tcttcgcacc agcatcaaga
     2881 tgcgcctgcg atttatcggc ggaggtataa aagccagtac attcgacaat gatttctgcc
     2941 cctttcgctt tccacggaat atttttagcc tctttttcgg cgtaaaccgc gatacttttc
     3001 ccatcaacga taagtgaatc ttccgtaaaa tcaacgctcc aggggaacgg accatagttt
     3061 gaatcatgtt tcagcagata ggcgagaatt tttggggaag taagatcatt aatagcgaca
     3121 acgtctatgt tgcttttgac ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca
     3181 aaaccgttaa taccaacttt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
     3241 tgatcctttc caggcttaga ccaggattgt gggatgggca atctccattc ttacagtgaa
     3301 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
     3361 gttaacggaa aattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
     3421 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
     3481 tcgtggtttc ttcccacgta gcgatcggcc gctcatcaac atgattcatg tttcctgtgg
     3541 catctcaatt ctcgtgctga tggtggttcg tcttttgtta agactgaaat acccaacccc
     3601 gccgattata cctaagccga aaccgatgat gacgggactg gcgcatttgg gacatttggt
     3661 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
     3721 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
     3781 ggttgatagc ttaaagtcgt ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg
     3841 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
     3901 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     3961 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     4021 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     4081 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgca
     4141 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     4201 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     4261 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcat
     4321 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     4381 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     4441 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     4501 gtgtgaatgc cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgcgct
     4561 tcatagcctt tctccttgcc ggatggcggg taagaggcta acaatctgaa ttgcttggtt
     4621 cattcgttgg cctcggtttg tactgacccc aaaaagttgg acagttaaac acgaggcata
     4681 taggtctgat tccgatattc atttggagtc agacctttta atttcaggct aattcttctg
     4741 ctgttgtagt attcaatata ttccgtaaca gcatccttca gttcgcttat attactgaac
     4801 t
//
LOCUS       DADLCQ010000215         4691 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000215 DADLCQ010000000
VERSION     DADLCQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.218"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..783)
                     /locus_tag="JQG95_003961"
     CDS             complement(<1..783)
                     /locus_tag="JQG95_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890338.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
     gene            875..961
                     /locus_tag="JQG95_003962"
                     /pseudo
     CDS             875..961
                     /locus_tag="JQG95_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(971..1051)
                     /locus_tag="JQG95_003963"
                     /pseudo
     CDS             complement(971..1051)
                     /locus_tag="JQG95_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1366..1842)
                     /locus_tag="JQG95_003964"
     CDS             complement(1366..1842)
                     /locus_tag="JQG95_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY5890339.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="JQG95_003965"
     CDS             complement(1901..3190)
                     /gene="bioA"
                     /locus_tag="JQG95_003965"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY5890340.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3277..4317
                     /gene="bioB"
                     /locus_tag="JQG95_003966"
     CDS             3277..4317
                     /gene="bioB"
                     /locus_tag="JQG95_003966"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY5890341.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4314..>4691
                     /locus_tag="JQG95_003967"
     CDS             4314..>4691
                     /locus_tag="JQG95_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY5890342.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRI"
BASE COUNT         1181 a         1144 c         1268 g         1098 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct
      901 ggccgaaaat gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg
      961 ttggtcgccc taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc
     1021 gcttatcgag atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat
     1081 tagcgcaaga ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca
     1141 tctaagtggt tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat
     1201 tttttaagct aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca
     1261 gcttttttat actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat
     1321 acctttaagt gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc
     1381 ggtaatcgat gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct
     1441 ggctccttca tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg
     1501 atgggtttcg cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg
     1561 cgtctgcaaa acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac
     1621 gcgggtatca gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc
     1681 atccgggtcg tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc
     1741 cagatgcggt gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg
     1801 caacttatcg ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt
     1861 tgcagaaagt gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt
     1921 catcctgtac cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa
     1981 tatagggcgg catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga
     2041 caaagaattt ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg
     2101 cccccagtac gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc
     2161 gcagctgcac ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta
     2221 ggctggcgtt tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa
     2281 agcagccggc ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg
     2341 aaagggtcat tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt
     2401 ctgcatattc acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg
     2461 caatcagcaa gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat
     2521 ggtacatgcg catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt
     2581 catgacgatg cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac
     2641 catccatgcg gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca
     2701 ggctgtgcat tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat
     2761 aaccattgcg gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca
     2821 acgccatttt catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact
     2881 ccagcggttg cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg
     2941 tgataccgcc aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa
     3001 gctgcgggtg attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc
     3061 gtctgccgtc agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca
     3121 gaggggaggt catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt
     3181 ccgttgtcat aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc
     3241 gaattaacaa caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca
     3301 ttgtcgcaag tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag
     3361 caggtgcatc gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt
     3421 aagaccggag cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc
     3481 gggctggaag ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg
     3541 aaagcggcag gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc
     3601 gatatgccgt acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt
     3661 atgacgctgg gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat
     3721 tactacaacc acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc
     3781 acttatcagg aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc
     3841 tcaggtggca ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa
     3901 ctggcaaacc tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa
     3961 ggcacgccgc ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc
     4021 gtagcgcgga tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg
     4081 aacgaacaga ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc
     4141 aaactgctga ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg
     4201 gggctaaatc cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt
     4261 ggacaggcgc tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct
     4321 ggcaggagaa aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc
     4381 gttatccggt ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga
     4441 acttttccag taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga
     4501 agcagggggc ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata
     4561 gcgtggtgca tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc
     4621 tgctgtttat ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag
     4681 aggaccgtat t
//
LOCUS       DADLCQ010000216         4636 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000216 DADLCQ010000000
VERSION     DADLCQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.219"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..311
                     /locus_tag="JQG95_003968"
     CDS             <1..311
                     /locus_tag="JQG95_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5890343.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="JQG95_003969"
     CDS             468..1217
                     /locus_tag="JQG95_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890344.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="JQG95_003970"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="JQG95_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY5890345.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /locus_tag="JQG95_003971"
     CDS             complement(1859..3196)
                     /locus_tag="JQG95_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY5890346.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLMSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="JQG95_003972"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="JQG95_003972"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY5890347.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..4557
                     /locus_tag="JQG95_003973"
     CDS             4084..4557
                     /locus_tag="JQG95_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY5890348.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1238 a         1162 c         1138 g         1098 t
ORIGIN      
        1 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agcctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcatcagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gttattgtac atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt
     4441 gacagtctcg ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc
     4501 attacccgtc tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg
     4561 ggatacttta tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc
     4621 aggccgttac cccaat
//
LOCUS       DADLCQ010000217         4550 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000217 DADLCQ010000000
VERSION     DADLCQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.220"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1224
                     /locus_tag="JQG95_003974"
     CDS             <1..1224
                     /locus_tag="JQG95_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY5890349.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1898
                     /locus_tag="JQG95_003975"
     CDS             1224..1898
                     /locus_tag="JQG95_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5890350.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPA"
     gene            2426..3187
                     /locus_tag="JQG95_003976"
     CDS             2426..3187
                     /locus_tag="JQG95_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY5890351.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="JQG95_003977"
     CDS             3231..4310
                     /locus_tag="JQG95_003977"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5890352.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="JQG95_003978"
     CDS             4307..>4550
                     /locus_tag="JQG95_003978"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY5890353.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1249 a          864 c         1119 g         1318 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgtaggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DADLCQ010000218         4398 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000218 DADLCQ010000000
VERSION     DADLCQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.221"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            52..1265
                     /locus_tag="JQG95_003979"
     CDS             join(52..376,376..1265)
                     /locus_tag="JQG95_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc25 family transposase"
                     /protein_id="HAY5890354.1"
                     /translation="MTKNMRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTP
                     ETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLWKKLAPLVDTLRGTHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLK
                     PEIQRVYDENHSVYGVRKVWRQLLREEVTVARCTVERLMKVMGLAGVRRGKKVRTTVS
                     RKEAAAGDRVNRQFVAECPNQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSS
                     MKTTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKA
                     YYASIGNGDLAA"
     gene            complement(1461..3041)
                     /locus_tag="JQG95_003980"
     CDS             complement(1461..3041)
                     /locus_tag="JQG95_003980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890355.1"
                     /translation="MNNKYLNKYITSAIGLVAVFICFYISWMPVFHHGSWSGHDFLFH
                     INRVLSSVNGLKNGQLIPYYDVDYKNHPGYGSNLFYPPLANLLGSIFFYISHDFNVTV
                     KLLAIFIAISSFLTSYFVLIRINGDKLPSVIYSLCFSCSIYMVDNIFIRSSYPEAMAI
                     CAIPLFLYGLGYASNRESVFFLASANIIFILSNIPATLCSGVVFIIYYSINRRRLAQY
                     VVSVCISILLCSWYVFPLIYSTHGESFTMLDRDWFPTMSRTYISLYDLISGEIIKSGP
                     LKGMALGIGLPITFAFIWNFATDHNKKKQDYLLVLIILLIICGGINFGFLGGVFTHLS
                     KIQFTWRFIPFLLFSILLILHASGRIKLKYILLIFTSTCLMTSAITVPKKSSHNLTID
                     IAASANYKDYVLSNAPELGDRIKELKCDNGLSYAFSRTLGSNGLPVFTFSIPTKTTCS
                     IPFMSYKSLELDGAKKYSRDGYFKAELTKGNYIIRIDTTYRFKQVVLISFIVSICTIL
                     TILLFALKRKWKWKWKWK"
     gene            complement(3038..3964)
                     /locus_tag="JQG95_003981"
     CDS             complement(3038..3964)
                     /locus_tag="JQG95_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY5890356.1"
                     /translation="MKISLVVPVFNEEATIPIFYKTVREFEELQQYDVEIVFINDGSK
                     DGTESIINSIASWDPLVVALSFTRNFGKEPALFAGLDHATGDAIIPIDVDLQDPIEVI
                     PHLITKWQAGADMVLAKRTDRSTDGRLKRKSAEWFYKLHNKISSPKIEENVGDFRLMS
                     RDVVENIKLMPERNLFMKGVLSWVGGKTDVVEYARAERFAGDSKFNGWKLWNLALEGI
                     TSFSTFPLRMWTYIGLFVAGISFFYGVWMIFDTFAFGNPVRGYPSLLVSILFLGGIQL
                     IGIGALGEYIGRIYVETKSRPKYILKKWKSQK"
     gene            complement(3961..4323)
                     /locus_tag="JQG95_003982"
     CDS             complement(3961..4323)
                     /locus_tag="JQG95_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY5890357.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYGLSTSQALANFAGFVV
                     AVSFSFYANARFTFKASTSTLRYMLYVGFMGTLSAAVGWAADRCGLPPIITLIAFSAI
                     SLVCGFFYSKFIVFREAK"
BASE COUNT         1343 a          936 c          966 g         1153 t
ORIGIN      
        1 atcgccccgg gaatcctgga gactaaactc cttgagagag aggcaaacag gatgactaaa
       61 aatatgcgct tttcccctga ggtccgtcag cgggccgttc gtatggttat cgaaagccag
      121 aatgactatg actcgcagtg ggcggccatc agctccatcg cccctaaaat cggctgtacg
      181 cctgagaccc tgcgtacctg gctgcgtcag tatgagcgcg atgccggtgc aggtgacggc
      241 ggactgagca ccactgaacg ccagcgtctg aaggaactgg agcgtgaaaa ccgcgaactg
      301 cgtcgcagca acgatatcct gcgccaggct tccgcgtatt ttgcgaaggc ggagttcgac
      361 cgcctgtgga aaaaatagcg cctctggtgg ataccctgcg tggtacgcac ggagtcggac
      421 cggtatgcca cgagctggct gtcgccccgt caacgtatta ccggcatcgt gcgcatcaac
      481 ggcatccgga taaacgcagt cagcgggcac agcgtgatga cctgctgaag ccggagatac
      541 agcgcgtgta cgatgaaaac cacagcgtgt acggtgtgcg caaggtctgg cgccagttgt
      601 tgcgtgagga ggtgaccgtg gccaggtgca cagtagaacg gctgatgaag gtgatggggc
      661 ttgccggtgt gcgccggggt aaaaaggtcc gcaccacagt cagccggaaa gaagccgctg
      721 cgggtgaccg ggtgaaccgc cagttcgtgg cagaatgccc caaccagctg tgggtggctg
      781 acttcacgta tgtgagcacc tggcagggct tcgcttacgt ggcgttcatc atcgatgtgt
      841 tcgccggcgt cattgtgggc tggcgggtct catcgtcgat gaaaacgacg ttcgtgctgg
      901 atgcactgga gcaggcgctg tgggcccgtc gcccgtcggg caccatccat cactcagata
      961 aggggtcaca atatgtgtcg ctggcataca ctcagcggct taaggatgca gagttgctgg
     1021 cgtcaaccgg cagcacaggc gactcatatg acaacgcgat ggcggagagc atcaacggac
     1081 tttataaagc ggaggtgata catcgtaaga gctggaaaaa ccgcgccgaa gtggaactgg
     1141 ccacactgac gtgggtggac tggtacaata accgacggtt gctgggacga ctgggtcata
     1201 tcccgccggc agaagcggaa aaggcatatt acgcttccat cggaaatggt gatctggcag
     1261 cctgagttca cagacaaaac actctcccgg aaaacagggg cggttcattg cgatgtagag
     1321 ccatatgatt tgatcgacaa agacccgggc atgcaaatgt ccttgtcttt gtcgttatct
     1381 gacaggatca atcaccgcct ccccaggagg tggttttggg gtgacaacaa atatggtaaa
     1441 aaattaactt ttattgttaa tcatttccat ttccatttcc atttccattt cctttttaaa
     1501 gcaaataaca atatggtaag tattgtgcat attgatacta tgaaagatat taatacgacc
     1561 tgtttgaacc tataagttgt gtcaatacgt ataatataat tacctttagt caattccgct
     1621 ttgaaatagc cgtctctcga atattttttt gccccatcta attctaatga tttataagac
     1681 atgaaaggta tgctgcaagt ggtttttgtt ggaatactaa atgtgaaaac aggtaaccca
     1741 tttgaaccta aggttcgtga gaaagcgtat gacagcccat tatcgcattt taattcttta
     1801 atccggtcac caagctctgg tgcatttgat agaacataat ctttataatt tgcactggcg
     1861 gcaatatcaa ttgtgaggtt gtggctactt tttttaggta cagtaatcgc acttgtcatc
     1921 aaacatgttg atgtgaaaat taacaagata tattttaatt tgattcttcc tgatgcatgg
     1981 agaataagta aaatgctaaa tagcaggaat ggaataaaac gccaagtaaa ttgaattttt
     2041 gataaatgtg taaatacgcc acctagaaaa ccaaaattaa tgccaccaca aatgattagc
     2101 aatataatta aaacaagcag gtaatcttgt ttctttttat tgtgatctgt cgcaaagttc
     2161 catataaatg caaaggtaat tggcagtccg atccctaatg ccattccctt tagggggcct
     2221 gattttatta tttctccaga aataagatcg taaagtgata tgtatgttcg tgacattgtt
     2281 gggaaccagt cacgatctaa catagtaaat gactcaccat gcgttgaata tatcaatggg
     2341 aatacatacc aactacataa tagtatagat atacaaacgc taacaacata ctgagctaat
     2401 ctacgtctat ttatggagta atatatgata aatacaaccc cagaacataa agttgcaggg
     2461 atattggata aaataaaaat aatattcgca gaagctaaaa aaaatactga ttctctgttt
     2521 gatgcatatc ctagcccata taaaaataac ggaatggcgc atattgccat agcttctgga
     2581 taagatgatc ttatgaaaat attatcaacc atatagattg agcatgaaaa gcacaatgaa
     2641 taaattactg atggtaattt gtccccattt attctaatta aaacaaagta tgatgtaaga
     2701 aatgacgata tcgcaataaa tatcgctaat aacttaacgg taacattgaa atcgtgactt
     2761 atataaaaaa atatagaacc taataaattt gctaaaggtg gataaaataa attactccca
     2821 tacccagggt gatttttata gtcaacatca taatatggga ttaattgacc attctttaat
     2881 ccattgactg atgataaaac tctattaata tgaaataaaa aatcgtgacc tgaccagctt
     2941 ccatggtgaa agactggcat ccacgatata taaaagcaaa taaaaacagc aaccaagcca
     3001 atagcacttg taatgtattt gtttaagtat ttgttattca tttctgactt ttccattttt
     3061 ttagtatgta tttaggtctg gattttgttt caacatatat tcttcctata tactcgccaa
     3121 gggcgccaat gccaatcagt tgaatccctc ctagaaataa tatagatacc aaaagtgatg
     3181 ggtatccgcg aacagggtta ccgaaggcga atgtatcaaa aatcatccat acgccataaa
     3241 agaatgaaat acctgctaca aacaagccaa tatatgtcca catgcgtaat gggaaagttg
     3301 agaaacttgt tatgccttca agcgcaaggt tccatagttt ccatccattg aacttggaat
     3361 caccagcgaa gcgttctgct cgcgcatatt ctacaacatc agttttaccg ccaacccaag
     3421 acagaacgcc tttcataaag aggtttcttt caggcatcag tttaatattt tcaacaacgt
     3481 cacgtgacat taacctgaaa tcgccaacat tttcttctat ctttggtgag ctgattttat
     3541 tgtgaagctt gtaaaaccat tccgctgatt tacgctttaa tcgtccgtca gttgaacgat
     3601 ccgttcgttt agcaagaacc atatcagccc cagcctgcca tttggtaatg aggtgaggaa
     3661 ttacttcaat aggatcttgc aggtccacat caatcggaat gattgcatca ccggttgcat
     3721 ggtccagccc agcaaacaga gctggttcct tcccaaaatt ccgggtgaac gataatgcaa
     3781 ccacaagagg atcccaagag gcaatagaat ttataattga ctctgttcca tctttactgc
     3841 cgtcattgat gaatactatt tcaacatcat attgctgtaa ctcttcaaac tctcttaccg
     3901 ttttataaaa gatcggtatc gtggcttcct cattgaagac cggaacgacc agagaaattt
     3961 tcattttgct tccctaaaaa caatgaattt tgaatagaag aaaccgcaaa caagactgat
     4021 tgcggagaat gctattaacg tgatgatggg tggtaacccg cacctgtcag cagcccaacc
     4081 tacagcagcg ctcagggtac ccataaagcc aacatagagc atgtaacgta gcgtggaggt
     4141 ggatgcttta aatgtgaaac gtgcattggc ataaaagctg aaactcaccg ccaccacgaa
     4201 gccagcaaag ttcgctaacg cttggcttgt tgacaatcca taaatgcaaa caccaaacac
     4261 cacccagtga atgagtgtat tgagaacccc aatcgatgtg tacttagcaa agagttttaa
     4321 cattagaaaa atcagtaaat tctgaaaaag tgagagttta gcatcaacag tgaagtggtc
     4381 aacgaaaact ggccaccg
//
LOCUS       DADLCQ010000219         4327 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000219 DADLCQ010000000
VERSION     DADLCQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.222"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..904)
                     /locus_tag="JQG95_003983"
     CDS             complement(<1..904)
                     /locus_tag="JQG95_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY5890358.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNV"
     gene            964..1242
                     /locus_tag="JQG95_003984"
     CDS             964..1242
                     /locus_tag="JQG95_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890359.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQG95_003985"
     CDS             complement(1243..1770)
                     /gene="gadE"
                     /locus_tag="JQG95_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY5890360.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQG95_003986"
     CDS             complement(2569..3141)
                     /gene="hdeD"
                     /locus_tag="JQG95_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY5890361.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3396..3728
                     /gene="hdeA"
                     /locus_tag="JQG95_003987"
     CDS             3396..3728
                     /gene="hdeA"
                     /locus_tag="JQG95_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY5890362.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3844..4170
                     /gene="hdeB"
                     /locus_tag="JQG95_003988"
     CDS             3844..4170
                     /gene="hdeB"
                     /locus_tag="JQG95_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY5890363.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4234..>4327
                     /locus_tag="JQG95_003989"
     CDS             4234..>4327
                     /locus_tag="JQG95_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtC family protein"
                     /protein_id="HAY5890364.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAG"
BASE COUNT         1274 a          924 c          926 g         1203 t
ORIGIN      
        1 atacattctg gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt
       61 caccatttgg gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg
      121 tcgggtcgga gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga
      181 gctgtactgg cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca
      241 tgcgtaagaa atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta
      301 cggtaaccag cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt
      361 tcccgctgac gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct
      421 gttcaacagc cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg
      481 cggtgtcgta atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa
      541 aggtgatgcg ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt
      601 ttagctcggc ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt
      661 tatcgccttc gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg
      721 caacttcata aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg
      781 agagtgtgac gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat
      841 cgcaggcggt gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt
      901 tcattttagt ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga
      961 gatatgaggg atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac
     1021 aggcacagtg atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca
     1081 gttcctgcca gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat
     1141 caagtcgttt tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc
     1201 cctcgtcatg ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat
     1261 acccagggtg acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt
     1321 ctgaatcttt aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt
     1381 atttttcaga atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt
     1441 cttatggggc aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg
     1501 gctaacaaaa taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac
     1561 aataattctc tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg
     1621 attgtgatag gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga
     1681 cagggaattg atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag
     1741 aaaagaatct ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa
     1801 catgtcatcc ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc
     1861 ctataagaac tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga
     1921 tttatccatc acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt
     1981 gtaaatagta aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata
     2041 accagtttgt gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc
     2101 gagtctcctt gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg
     2161 aaattacctg agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca
     2221 cggatattta tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt
     2281 taaccgccca tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta
     2341 aaacattaaa cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg
     2401 ccatggagtg aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat
     2461 tatttcaatg atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg
     2521 gatcatagtc acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt
     2581 aacgaacaaa ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac
     2641 cagtgtggaa accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat
     2701 gacgatatcc agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg
     2761 ataccaactc atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc
     2821 aatggcaaaa atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc
     2881 gactgcgacg aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa
     2941 cccgacaata agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat
     3001 atcgccagag acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc
     3061 aataaactgg attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt
     3121 tgccttatct atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta
     3181 tcaatctatg ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg
     3241 cagtatctga tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt
     3301 tccatcaaca tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt
     3361 gacggctctt tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc
     3421 ttggtggtct gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg
     3481 ataacaaaaa accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct
     3541 tccagccaac tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg
     3601 cggttttaga tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc
     3661 aggataaaca agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag
     3721 atatgtaatt ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata
     3781 tcttctttaa aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac
     3841 aaaatgaata tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg
     3901 tcactggtga acgcacaatc tgcgctggca gccaatgaat ctgctaaaga tatgacctgc
     3961 caggaattta ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat
     4021 gaagaaacag tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa
     4081 attcctaaag tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa
     4141 aatcaagcat ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt
     4201 gagccatctt gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt
     4261 atactcgcgg caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga
     4321 gcagggt
//
LOCUS       DADLCQ010000220         4291 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000220 DADLCQ010000000
VERSION     DADLCQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.223"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            29..910
                     /gene="yddG"
                     /locus_tag="JQG95_003990"
     CDS             29..910
                     /gene="yddG"
                     /locus_tag="JQG95_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY5890365.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1165..2265
                     /gene="ompC"
                     /locus_tag="JQG95_003991"
     CDS             1165..2265
                     /gene="ompC"
                     /locus_tag="JQG95_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY5890366.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            2429..2914
                     /locus_tag="JQG95_003992"
     CDS             2429..2914
                     /locus_tag="JQG95_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY5890367.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            3024..4013
                     /locus_tag="JQG95_003993"
     CDS             3024..4013
                     /locus_tag="JQG95_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY5890368.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         1329 a          880 c          817 g         1265 t
ORIGIN      
        1 taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct
       61 aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct
      121 cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac
      181 ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt
      241 attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca
      301 ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat
      361 tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt
      421 cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa
      481 tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc
      541 ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct
      601 gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt
      661 tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatac
      721 tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt
      781 tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc
      841 gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg
      901 tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa
      961 tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc
     1021 acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc attctatttt
     1081 aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa
     1141 cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt
     1201 ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg taataaactc
     1261 gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg tgacgatggt
     1321 gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt
     1381 ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa cggctcgcgc
     1441 ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg ctctctggac
     1501 tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat gttgccagaa
     1561 tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac caccggtcta
     1621 gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt tgccgcgcaa
     1681 tatcaaggca aaaatgaacg tactgacaac agtcatcttt atggtgctga ctacacgcgt
     1741 gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt tggtatcggt
     1801 gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc taatcctctc
     1861 aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga tgccaacaac
     1921 atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg ggatggttat
     1981 atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga cttcggcttg
     2041 cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag
     2101 gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc
     2161 tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc cgtagatatt
     2221 cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc tctcctgacc
     2281 agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt aagtaaaaat
     2341 gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat atcctccggg
     2401 gacatcttac tataatattt attgatgagt gattatgacc aatattaata cagcttgtgt
     2461 aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa ccatctccag
     2521 caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg gtgaagaaag
     2581 agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat ctcttgattt
     2641 gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata cgttaaatct
     2701 ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg ttaatatcaa
     2761 cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac tggaaaacat
     2821 ttatattgat cataacaatc tggaaagttt agatttaaaa aatcagcatt cattggttaa
     2881 tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat aaactgaaat
     2941 ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa gaatctctga
     3001 ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga
     3061 tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa
     3121 aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac
     3181 taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat gatccacttg
     3241 cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca
     3301 ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta
     3361 tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa
     3421 agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatgtaa
     3481 gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata gataggcttt
     3541 catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa ttagaatctt
     3601 gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt gttgcaggta
     3661 ataaaaatga actcttactg ctggatctat cccataataa actaacaagt ttacacaatg
     3721 tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct gaaattaaaa
     3781 tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac cagttgaaat
     3841 atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga ctggacaata
     3901 ataaaataac ccacattgat actaataata catccgatat tggaacttta ttccccataa
     3961 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgacctacta
     4021 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
     4081 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat aacgaattgt
     4141 ttcactatac tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
     4201 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac cttagggtaa tgctgccaac
     4261 ttactgattt agtgtatgat ggtgatttta a
//
LOCUS       DADLCQ010000221         4183 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000221 DADLCQ010000000
VERSION     DADLCQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.224"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..209)
                     /locus_tag="JQG95_003994"
     CDS             complement(<1..209)
                     /locus_tag="JQG95_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5890369.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JQG95_003995"
     tRNA            complement(324..399)
                     /locus_tag="JQG95_003995"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JQG95_003996"
     tRNA            complement(406..480)
                     /locus_tag="JQG95_003996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JQG95_003997"
     tRNA            complement(597..681)
                     /locus_tag="JQG95_003997"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JQG95_003998"
     tRNA            complement(690..765)
                     /locus_tag="JQG95_003998"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JQG95_003999"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JQG95_003999"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY5890370.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQG95_004000"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JQG95_004000"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY5890371.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGEMLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQG95_004001"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JQG95_004001"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY5890372.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVCFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1039 c          928 g         1134 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcat ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcaga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DADLCQ010000222         4176 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000222 DADLCQ010000000
VERSION     DADLCQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.225"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..632
                     /gene="xerC"
                     /locus_tag="JQG95_004002"
     CDS             <1..632
                     /gene="xerC"
                     /locus_tag="JQG95_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY5890373.1"
                     /translation="QNELKANPAKGVSAPKAPRHLPKNIDVDDMNRLLDIDINDPLAV
                     RDRAMLEVMYGAGLRLSELVGLDIKHLDLESGEVWVMGKGSKERRLPIGRNAVAWIEH
                     WLDLRDLFGSEDDALFLSKLGKRISARNVQKRFAEWGIKQGLNNHVHPHKLRHSFATH
                     MLESSGDLRGVQELLGHANLSTTQIYTHLDFQHLASVYDAAHPRAKRGK"
     gene            632..1348
                     /gene="yigB"
                     /locus_tag="JQG95_004003"
     CDS             632..1348
                     /gene="yigB"
                     /locus_tag="JQG95_004003"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY5890374.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            1432..3594
                     /gene="uvrD"
                     /locus_tag="JQG95_004004"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             1432..3594
                     /gene="uvrD"
                     /locus_tag="JQG95_004004"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY5890375.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(3741..4172)
                     /locus_tag="JQG95_004005"
     CDS             complement(3741..4172)
                     /locus_tag="JQG95_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890376.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT          995 a         1107 c         1188 g          886 t
ORIGIN      
        1 gccagaacga actcaaagct aacccggcga aaggagtttc ggcaccgaaa gcgccgcgtc
       61 atctgccgaa aaatatcgac gtcgacgata tgaatcggct gctggatatt gatatcaacg
      121 atcccctcgc tgtacgcgac cgtgcaatgc tggaagtgat gtacggcgcg ggtctgcgtc
      181 tttccgagct ggtggggctg gatatcaaac acctcgacct ggagtccggc gaagtgtggg
      241 tgatggggaa aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga
      301 ttgagcactg gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt
      361 cgaaactggg caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca
      421 taaaacaagg gctgaataat cacgttcacc cacataaatt acgtcactcg ttcgctacgc
      481 atatgctgga gtcgagcggc gatcttcgtg gtgtgcagga gctactgggt catgccaacc
      541 tctccaccac gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg
      601 cggcgcatcc acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat
      661 ctcggcgctc acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg
      721 caccgagcga gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca
      781 gaatgaagat ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca
      841 cgacgtgaca cgttggcgtt ttcgttcgat tgaacaagcg atgctcgacg ccgggctgag
      901 tgccgaagaa gccagtgcag gcgcacacgc agcaatgatt aactttgcca aatggcgcag
      961 ccgaatcgac gtcccgcagc aaactcacga caccttaaaa cagctggcga agaaatggcc
     1021 gctggtggcg atcaccaacg gtaacgccca gccggaactg tttggtttgg gggattattt
     1081 tgagtttgtg ctgcgcgctg gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt
     1141 tttggctgcg gaaaaactca acgtgccgat cggcgagatc ttacatgttg gggacgatct
     1201 caccactgac gtgggtgggg caattcgcag cggaatgcag gcttgttgga tcagaccgga
     1261 aaatggcgat ctgatgcaaa cctgggacag ccgtttactg ccacatctgg aaatttcccg
     1321 gttggcatct ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg
     1381 gcggagggcg ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt
     1441 tcttacctgc tcgacagcct taatgacaaa caacgcgaag cggtggccgc gccacgcagc
     1501 aaccttctgg tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc
     1561 gcctggctga tgagcgtgga aaactgctcg ccatactcga ttatggctgt gacgtttacc
     1621 aacaaagcgg cggcggagat gcgtcatcgt atcggacaac tgatgggcac cagccagggc
     1681 ggcatgtggg taggcacctt ccacgggctg gcgcaccgcc tgctgcgtgc gcaccatatg
     1741 gacgccaatc tgccgcagga tttccagatc ctcgatagcg aagaccagct acgcctgctt
     1801 aagcgtctga tcaaagccat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg
     1861 tggtacatca acagccagaa agatgaaggc ctgcgtccgc atcatattca aagctacggt
     1921 aatccggtgg agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgcgatcgc
     1981 gcgggcctgg tggacttcgc cgagctgctg ctgcgcgctc acgaactgtg gcttaacaag
     2041 ccgcatatcc tacaacacta ccgcgaacgt tttaccaata tcctggtgga cgaattccag
     2101 gataccaaca acattcagta cgcgtggatc cgcctgctgg cgggcgacac cggcaaagtg
     2161 atgatcgtcg gtgatgacga ccagtcaatc tacggctggc gcggggcgca ggtggagaat
     2221 attcagcgtt tccttaatga tttccccggt gccgaaatta ttcgtctgga gcaaaactac
     2281 cgctctacca gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt
     2341 ctgggtaaaa aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct
     2401 tttaacgaac tcgatgaagc gcgttttgtg gttaaccgca tcaaaacctg gcaggacaac
     2461 ggcggggcgc ttgccgagtg cgccattctc taccgcagta acgcccagtc gcgtgtgctg
     2521 gaagaagcac tgttgcaggc gagtatgccg taccgtattt acggtgggat gcgcttcttc
     2581 gaacgccagg aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgtaacgac
     2641 gacgcggcct ttgagcgtgt ggtgaatacg ccaacgcggg gtattggtga ccggacgctg
     2701 gacgtggtac gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa
     2761 ctgttgcagg aaaaagccct cgccggacgt gctgccagcg ccttacagcg gtttatggaa
     2821 ctgatcgacg ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg
     2881 gtaattaaag actccggcct gcgtaccatg tacgagcagg agaagggcga aaaaggtcag
     2941 acgcgtatcg aaaacttaga ggaactggtg acggcaacgc gccagttcag ctacaacgaa
     3001 gaagacgaag atttaatgcc gctgcaggcg ttcctatccc atgcggcgct ggaagcaggc
     3061 gaagggcagg cggatacctg gcaggatgcg gtacaactga tgaccatgca ctcggcgaaa
     3121 ggtctggagt tcccgcaggt gtttatcgtc ggtatggaag agggcatgtt cccaagccag
     3181 atgtcgctgg atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta
     3241 acccgcgcga tgcagaaact gacgctgacc tacgcggaaa ctcgccgtct gtatggcaaa
     3301 gaggtttacc atcgcccgtc gcgctttatt ggcgagttgc cggaagagtg tgtggaagag
     3361 gtgcgcctgc gcgccacggt aagccgcccg gtcagccatc agcggatggg tacgccgatg
     3421 gtcgagaacg acagcggcta caagctcggc cagcgtgtac gccacgctaa gtttggtgaa
     3481 ggcaccatcg tcaatatgga aggcagcggt gaacatagcc gtttgcaggt ggcattccag
     3541 ggccagggaa tcaaatggct ggtggcagct tacgcccggc tggagtcggt gtaacgttgc
     3601 cggatgcggt gctgcgcacc ttatttggcc taaaaaatca ttcagattca ataaattgca
     3661 acgtcatgta ggccggataa ggcgtttacg ccgcatccgg catcagcagc atatttagta
     3721 tctgacacaa agctatcatt ctaacctttc cgctcgacgg aaatcatggt gacaaaagga
     3781 tagcgttgcc acgggattgc cccgcctttc atatacatat gtgaaatcgt gccaccaaga
     3841 taaagcagct gctcaacgtt cagtttcgct ttggcataac aggcaaaatc ataaaaattt
     3901 gtcgcctgct ggctcaacaa aaacacggca ttcccatgtt tattaatccc aacaccgtta
     3961 cgaattttac gtgaggcgac gttgggatga atacgcggat taattacacc gttttccatc
     4021 aacattggtc ctgactgcac cgcaaactga atatctttac tggctttaaa agcatccaga
     4081 cgaacgatgc cgactttatc tcccgcgaca taaaacacgc cgccaggacg gataaagaaa
     4141 ttcccttcac ctgaagcgag atttaacgcc accttc
//
LOCUS       DADLCQ010000223         4162 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000223 DADLCQ010000000
VERSION     DADLCQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.226"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..332)
                     /locus_tag="JQG95_004006"
     CDS             complement(<1..332)
                     /locus_tag="JQG95_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY5890377.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..1714)
                     /locus_tag="JQG95_004007"
     CDS             complement(332..1714)
                     /locus_tag="JQG95_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY5890378.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(1711..2331)
                     /locus_tag="JQG95_004008"
     CDS             complement(1711..2331)
                     /locus_tag="JQG95_004008"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890379.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTTLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(2356..3549)
                     /gene="flhB"
                     /locus_tag="JQG95_004009"
     CDS             complement(2356..3549)
                     /gene="flhB"
                     /locus_tag="JQG95_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY5890380.1"
                     /translation="MSDESDDKTEAPTPHRLEKAWEEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQGEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            3785..>4162
                     /locus_tag="JQG95_004010"
     CDS             3785..>4162
                     /locus_tag="JQG95_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY5890381.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPF"
BASE COUNT         1037 a         1206 c         1019 g          900 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcggtta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacgtagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gccttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     3481 ctcttcccac gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     3541 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
     3601 cgttcaatgg tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata
     3661 ggttcagttg cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc
     3721 tatagaatcg cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga
     3781 tcaaatggaa tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac
     3841 caacatagca tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga
     3901 ccgattactt tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt
     3961 agatcaaaat gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc
     4021 tcgcgggata ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc
     4081 cacggggatt gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca
     4141 cgatctcggt aatccaccat tt
//
LOCUS       DADLCQ010000224         4144 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000224 DADLCQ010000000
VERSION     DADLCQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.227"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..158)
                     /locus_tag="JQG95_004011"
     CDS             complement(<1..158)
                     /locus_tag="JQG95_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY5890382.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JQG95_004012"
     CDS             complement(308..469)
                     /locus_tag="JQG95_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890383.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JQG95_004013"
     CDS             complement(466..669)
                     /locus_tag="JQG95_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY5890384.1"
                     /translation="MLERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JQG95_004014"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JQG95_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY5890385.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JQG95_004015"
                     /pseudo
     CDS             complement(1530..1661)
                     /locus_tag="JQG95_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
     gene            complement(2161..2237)
                     /locus_tag="JQG95_004016"
     tRNA            complement(2161..2237)
                     /locus_tag="JQG95_004016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JQG95_004017"
     tRNA            complement(2339..2414)
                     /locus_tag="JQG95_004017"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="JQG95_004018"
     CDS             complement(2559..3380)
                     /locus_tag="JQG95_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY5890386.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="JQG95_004019"
     CDS             complement(3395..3751)
                     /locus_tag="JQG95_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY5890387.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4144)
                     /locus_tag="JQG95_004020"
     CDS             complement(3764..>4144)
                     /locus_tag="JQG95_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY5890388.1"
                     /translation="PLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKY
                     KRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFE
                     EKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1156 a         1123 c          809 g         1056 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgggttctg ttgctggtgg gcgtgaggca
     4141 gcgg
//
LOCUS       DADLCQ010000225         4087 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000225 DADLCQ010000000
VERSION     DADLCQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.228"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            157..840
                     /locus_tag="JQG95_004021"
     CDS             157..840
                     /locus_tag="JQG95_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY5890389.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGVELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            841..1062
                     /locus_tag="JQG95_004022"
     CDS             841..1062
                     /locus_tag="JQG95_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890390.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            1076..1510
                     /locus_tag="JQG95_004023"
     CDS             1076..1510
                     /locus_tag="JQG95_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY5890391.1"
                     /translation="MYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            1555..1879
                     /locus_tag="JQG95_004024"
                     /pseudo
     CDS             1555..1879
                     /locus_tag="JQG95_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428429.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2198..2768
                     /locus_tag="JQG95_004025"
                     /pseudo
     CDS             2198..2768
                     /locus_tag="JQG95_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            2741..3166
                     /locus_tag="JQG95_004026"
     CDS             2741..3166
                     /locus_tag="JQG95_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY5890392.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            3213..3635
                     /locus_tag="JQG95_004027"
     CDS             3213..3635
                     /locus_tag="JQG95_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY5890393.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            3632..3823
                     /locus_tag="JQG95_004028"
     CDS             3632..3823
                     /locus_tag="JQG95_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890394.1"
                     /translation="MNINTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT          839 a         1125 c         1254 g          869 t
ORIGIN      
        1 ttcgcggcat aaccttgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga
       61 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc acaggggtgc
      121 cctcccggtg tcctctgtaa gagaaggagt gctgttatgt cccgttttat cctgggtgac
      181 tgcgtgcgtg ttatggccac gtttccgggc aatgctgtcg atttcatcct caccgacccg
      241 ccgtatctcg tcggtttccg tgaccgttcc gggcgcacca tcgccggcga taaaaccgat
      301 gaatggctgc aaccggcctg taatgaaatg taccgcgtgc tgaaaaaaga tgcgctgatg
      361 gtcagcttct acggctggaa ccgcgtcgat cgctttatgg cagcctggaa aaatgcggga
      421 ttcagcgtgg ttggccacct ggtgtttacc aaaacctaca catcgaaggc cgcatatgtg
      481 ggctatcgcc acgaatgcgc ctacatcctg gcaaaaggac gtccggcgct gcctcagaag
      541 ccattgcccg acgtgctggg ctggaaatat tcaggcaatc gtcatcaccc gactgaaaag
      601 cccgttacca gcctgcaacc gctcattgag agcttcacac acccgaacgc gattgtgctg
      661 gacccgtttg caggcagcgg ctcaacctgt gtggccgccc tccagtccgg acgccggtat
      721 atcggtgtcg agctgcttga acagtatcac cgtgccggac agcaacgact ggccgccgtg
      781 caacgggcca tgcagcaggg ggccgcgaat gataactggt ttgaaccgga ggctgcgtaa
      841 atgaactatg ccggacacga aaaacttcgc gccgacgtgg cggaggtggc caacaccatg
      901 tgtgaccttc gggccaggct gaacgatatg gagcatcgct gtcgctttga ttccgatgtg
      961 cttgttgaac gcctgacacg ccagacgctg tatcgcgcca atcgcctgtt catggaggcg
     1021 tataccgaaa ttctggaact ggattcctgc ttcaaagatt gagaaggaga cgagaatgta
     1081 cggaacatgc gaaacgctat gccgggagct ggcagcaaag tatccgggag acacgccgct
     1141 gatgctggtt atctggtccc cggaagaaat tcaggccctc gctgacggaa tggatatttc
     1201 cctgtccgat catgaaatca ggactgtcct ggcgcgcctg gaggacatcc cggaagacca
     1261 gcggactgaa tccggtattt cttccggtgc ggcgatggag atcatcaata acgtgagaga
     1321 aaaccgccag gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca
     1381 ggcattgtgg aaacgtgaat gggccgcccg ggatcatggc ctcgccgtcc cggaatgcgt
     1441 cacccgccgt caggcggtgg ttaatcaggt ccgcatcctg ctgaaaaaca acacacacga
     1501 aaacgactga tgttatcgcc gcctccgggc ggcgatacag ggagttcatg aattatgaat
     1561 gaaacgctga atgcactgat ttatcgccac gccagcaacc tgttgctggc gcagggctgg
     1621 ccggaagaga cggatgttga ccagcgtaac ccgaagtatc cgggctggat cagcatttac
     1681 gtgcggctgg atgcgccccg gctggcgacg ttacttatca accgtcacgg cggcgtactg
     1741 ccgccgctcc tggcctctgc cattcagaaa ctgaccggaa ccggagcgga acttgttctg
     1801 tccggcagtc agtggcagtc gctgccggta cttccggcag acggaacgca ggtgtctttc
     1861 ccgtatgccg gtgagtggct ggcagaggac gaaatcaggg ccgttcttgg tgcggtacgc
     1921 gatgcagtat gcagtgtcag ttaccaggtg gcagaagatg cgcggcgtat ccgtgcggcg
     1981 ctgaccacca ccggtcagac gttgctgacc cgccagacgc gccgctttcg cctggtggtg
     2041 aaggaaagcg atcacccctg ctggctcgat gaagacgacg aaaacctgcc cgtggtgctc
     2101 gatgccatcc tgaaccgggg cgctcgtttt tcgtcggtgg aaatgtatct ggtcagtgag
     2161 tgtgtggagc atatcctgtc cagtgggctg gcctgcgata tgctgcgtat accggatgaa
     2221 ccgccccgcc gctggtttga ccgtggtgtt ctgcgggagg tggtccggga agcccggaac
     2281 gaaatccgca gcatggcgga ttcactggcg aaaatccgga aatgatgacg ctggcgggga
     2341 aaaatctccg ccttttccag ccggtccccg gaaagagcaa aaggatgtcg ccgcaggctc
     2401 cgctttaccc ggccatgcgg ggcatggcct tgtgggtttt cagttatgtg gcctcagcgt
     2461 cgtgtgcggg ctgtgccgtg cctccatcgt agccgggctt ctgcgggcgg gttgcaaggt
     2521 aaggtgaacc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
     2581 tcacccgctc agatttcgcg gtctcaccct ccaactcccg tcagccgtgc gcgtcaggct
     2641 gcggcttccc ttgcgccctt gcaaccctgc aggcccggca aagatggagg cacggcaccg
     2701 cccggtgtcg cggaatatgt gaacaacgga ggaaaacatc atgcaatatg cgaaacctgt
     2761 cactctgaat gttgaagagt gcgaccgtct ctcctttctg ccttacctgt ttggcctgga
     2821 ttttctgtat gccgaagcgt ctgtatacgc gctggcgaaa aaaatgatgc cggaatatga
     2881 aggcggattc tggcacttca tccgcctgcc ggacggtggc ggttacatga tgccggatgg
     2941 cgaccgtttc cacatggtga acggtgcaaa ctggtttgac cgtacggtca gcgccgatgc
     3001 cgcaggcatc attctgactt cccttgtgat taaccgccag ttgtggctgt accacgacag
     3061 cggggatgca ggactgaccc atctttaccg gatgcgcgat gcgcagttgt ggcgtcacat
     3121 cgaatttcac cccgaatgca acgcgattta cgcggcactg gactgattaa cggacggggc
     3181 ggcaatgccg cccctgtaaa ggagacgaga acatgtactg tactgttaaa gaaattatcc
     3241 gcgaagtgct ggatacagac gtgccggaca gtgaatgtgt ttttgccgta gtgctgaccc
     3301 gtggggatgt gcgccacata gcccaggact ggaacctgtc agacgatgag ctggaaaccg
     3361 tcatgcagcg gctggacgat gcctttgagc atggtgcgga tgtcagcgtt gttcacgacg
     3421 ttgttcgtga actgatggag gaaaagcacg ccagccgtca ggtgacagtc ccggcggtga
     3481 tgctggaaaa agtgatggcg ctggcaggca gtgaaatgaa acgcctgtat gccgtcggga
     3541 gtgagaacgg gggcgacggt gatgcgttcg tcagggaaga acgcgaagca atggacgttg
     3601 tgttacaggc gctggacggg gagcacatgt catgaatatc aacacggaaa cccgcgaaat
     3661 tctgcgtaat tacagggccg tgattaatgc gcggcgtcgt gaaatggggc agaaaccgct
     3721 caccaccgcg cagattgttg atgaaatctg cgattttgtg gcgaatcagc aggcggtttt
     3781 cctgggcggt cactacatct tacagggcag cagaaacagg tgatacaggg gcaggcggtg
     3841 gaggccgcct gtcagcccgc gcgacccccc ctggcaggct ggcgcctgcc ggaagggaag
     3901 cgggccacgc tggcgcgtcg cccgttaccc ttcccgtctg tgatgtgtct gactcaggag
     3961 tgtttctttc ttatgttatg gaactgttcc tgatgttgtt gttgttgttg ttcctttatc
     4021 tgctgctctg attctgacta ctgcagtgtt gttctgtctg ccggacgcac cttcggctcc
     4081 cggctgc
//
LOCUS       DADLCQ010000226         4077 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000226 DADLCQ010000000
VERSION     DADLCQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.229"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(6..197)
                     /locus_tag="JQG95_004029"
     CDS             complement(6..197)
                     /locus_tag="JQG95_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY5890395.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESFHDH
                     ADELYRSCLARFGDDGENL"
     gene            261..584
                     /locus_tag="JQG95_004030"
     CDS             261..584
                     /locus_tag="JQG95_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY5890396.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLCQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            574..885
                     /locus_tag="JQG95_004031"
     CDS             574..885
                     /locus_tag="JQG95_004031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890397.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVDAPGQGT
                     A"
     gene            860..1282
                     /locus_tag="JQG95_004032"
                     /pseudo
     CDS             860..1282
                     /locus_tag="JQG95_004032"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1263..1793
                     /locus_tag="JQG95_004033"
                     /pseudo
     CDS             1263..1793
                     /locus_tag="JQG95_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
     gene            1800..2033
                     /gene="mbeD"
                     /locus_tag="JQG95_004034"
     CDS             1800..2033
                     /gene="mbeD"
                     /locus_tag="JQG95_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY5890398.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2579..3046)
                     /locus_tag="JQG95_004035"
     CDS             complement(2579..3046)
                     /locus_tag="JQG95_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890399.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQKLQIQEVCNNTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          991 a          931 c         1181 g          974 t
ORIGIN      
        1 atatgtcaga ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca
       61 tcggcgtggt cgtgaaacga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
      121 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
      181 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgttcag gaggtaatga
      241 taccgatgaa acgcgagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
      301 tactggaacg ctgtgagggt aaacagctgg cggtatggat gcggcgggtc tgcctggggg
      361 agccggttgc ccgttccggg aaactgccga cactggcacc gccgttactg tgtcagctgg
      421 ctgccatcgg gaataacctg aatcagacag cgaggaaggt gaacagcggg cagtggtctt
      481 ccggtaaccg ggttcaggtg gtggccgcac tgatggccat cggggacgag ctgcgccggc
      541 tgcgtctggc cgtcagggaa caggggacgc gggatgatag ttaagtttca tgcccgggga
      601 aaaggtggtg gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa ccgggaaggg
      661 gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt
      721 gcgaagaaat acacgtccgg tgttctgtcg ttcgcggaga aggagctgcc gccgggagga
      781 cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag
      841 tacagcatcc tgtgggtgga tgcaccagga caagggacgg cttgagctga attttgtcat
      901 tccgaacatg gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag
      961 gcctagaatt gatgcctggc agacgctggt taatcactat tacgggctgc atgacccgaa
     1021 cgccccggag aaccgcagga cgctgacact ccctgataat ctgccggaaa cgaaacaggc
     1081 gattgctgag ggcgttacac gcgggataga cgcgctttac catgccggag agataaaagg
     1141 ccgtcaggat gtgattcagg ctctcacaga ggcagggatt gacgtggtta gggtgacgcg
     1201 aagcagtatc agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcgtt
     1261 ttatgagcaa tcttttacag acgggtgcgg agttcgagaa aaagctgaaa gagagagccg
     1321 aatctacaga gaaaatgctg aacgacgagt tcaggaagct cgaagaatct gtaaacaggg
     1381 aactgacctc aaacgaatct ttaatcagga acgctatcaa cgaccataca tcagccctga
     1441 aagaacttct ggagaggtat cagaaaacga ccgtcgatac gatggacgca cactggaaaa
     1501 ccgtactgaa aatgagcgtg aaacgctggc tgtggctgat tatcgtctca gtactgatgt
     1561 ttgccacgac cgggagcctg ctgtggtacc agggcatgaa gataaacgcg aacatgaata
     1621 ttctccgcga gtagaaagag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt
     1681 atcacgagga cagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga
     1741 actggacgat ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag taacagagca
     1801 tgacggagct ggaaagacat ttgctgaacg cattagaaca actgcaacag gactatatgc
     1861 agcggctgaa cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc
     1921 aacgggacaa cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgcagcact
     1981 tgagcgagca gacagaacgc ttgagccagt tatacagcga gaacaggaga taagggacga
     2041 acggctgata caggaatgcg aacatgcgtt atccctggag cgtgagcacc agctggaaat
     2101 acaggaacgg acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt
     2161 tgtcagcgcg tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg
     2221 cgcttcagtc agaccttgct gaaaacatag gaacgcgtta aaacgcatac gggcgcgatt
     2281 ctggtgtgtc ttttgttgct gtgttttgcc tctattgcca gatcgcgctc attcccctga
     2341 acttcttttt tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aaaaaaatcc
     2401 gtttcagctg tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat
     2461 gtggttaaaa tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt
     2521 acctgtctgc tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt
     2581 acgttaaatt gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt
     2641 agtgggtcga ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga
     2701 tgcaacagaa ggcgtgagat tctcctgggc ctggaaagga taagtgttta gaattgatgg
     2761 caaaatatct tccatccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgt
     2821 tgttacaaac ttcttgaatt tgtagttttt gcaggactct tattactgcg ttatcatgtt
     2881 ccaattcttt aggggagtgt attattctct cttttaataa gctgtttacc acagggttta
     2941 gttgttgtct aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca
     3001 tgtttggcgg ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc
     3061 ttatagcatc aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc
     3121 ttgagatcct tttttgcggc gcgtaatctg ctgcctgcaa acaaaaaaac caccgctacc
     3181 aacggtggtt tgtttgccgg aatctagagc taccaactct ttcttccgaa ggtaactggc
     3241 ttggtggagc acagatacca aatgctgtcc ttctagtgta gccgcagtta ggccaccact
     3301 tcaagaactc ttaatatctc aatccacctt gtccagttac caatggctgc tgccagtggc
     3361 gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcgg
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccatcgg cgcggccttc
     3721 ttcctgtccc tggtcttttg ctcacatgtt ctttccggcg ttatcccctg attctgtgga
     3781 taacagcatt tcgtttttga gtgagtagat accgctcgcc gcagtcgaac gaccgagcgt
     3841 agcgagtcag tgagcgagga agcggaaaag cgcctggacg tgcattttct ccttacgcat
     3901 ctgtgcggta tttcataccc ggtatggcgc acttttcata caatctgcac tgatgccgca
     3961 tggttaagcc cgtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     4021 acccgctaaa acctgctgat gcgccctgac gggcttgtct gctcccggca tccgctc
//
LOCUS       DADLCQ010000227         4032 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000227 DADLCQ010000000
VERSION     DADLCQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.230"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..430
                     /locus_tag="JQG95_004036"
     CDS             <1..430
                     /locus_tag="JQG95_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705992.1"
                     /note="catalyzes the dehydration of L-carnitinyl-CoA to
                     crotonobetainyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carnitinyl-CoA dehydratase"
                     /protein_id="HAY5890400.1"
                     /translation="VCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVMTGR
                     RMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEE
                     AYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            436..1026
                     /gene="caiE"
                     /locus_tag="JQG95_004037"
     CDS             436..1026
                     /gene="caiE"
                     /locus_tag="JQG95_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY5890401.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1112..1507)
                     /gene="caiF"
                     /locus_tag="JQG95_004038"
     CDS             complement(1112..1507)
                     /gene="caiF"
                     /locus_tag="JQG95_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY5890402.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(1769..>4032)
                     /gene="carB"
                     /locus_tag="JQG95_004039"
     CDS             complement(1769..>4032)
                     /gene="carB"
                     /locus_tag="JQG95_004039"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY5890403.1"
                     /translation="KVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIPRFNF
                     EKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEALT
                     KIRRELKDAGAERIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVG
                     ITGLNAEFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFAT
                     DTAYMYSTYEEECEANPSTDREKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYET
                     IMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARAL
                     EAAGVPVIGTSPDAIDRAEDRERFQHAVDRLKLKQPANATVTAIEMAVEKAKEIGYPL
                     VVRPSYVLGGRAMEIVYDEADLRRYFQTAVSVSNDAPVLLDHFLDDAVEVDVDAICDG
                     EMVLIGGIMEHIEQAGVHSGDSACSLPAYTLSQEIQDVMRQQVQKLAFELQVRGLMNV
                     QFAVKNNEVYLIEVNPRAARTVPFVSKATGVPLAKVAARVMAGKSLAEQGVTKEVIPP
                     YYSVKEVVLPFNKFPGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGR
                     ALLSVREGDKERVVDLAAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQ
                     DRIKNGEYTYIINTTSGRRAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATE
                     KVISVQEMHAQIK"
BASE COUNT          900 a         1099 c         1069 g          964 t
ORIGIN      
        1 tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aaactgggca tcgttcctga
       61 cagcggcggt gtgctgcgtc tgccgaagat cctgccgcct accatcgtca atgaaatggt
      121 gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt
      181 ggttagccag gcggaactga tggataacgc ccgcgaactg gctcagcagc tggttaacag
      241 cgccccgctg gcgattgcgg cgctgaaaga gatctaccgc accaccagcg aaatgccggt
      301 agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccat cggttctgca
      361 ttcggaagat gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa
      421 aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg
      481 acggcgtttg tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc
      541 tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga
      601 gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg
      661 gaaaacggcc atatcgggca tggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca
      721 ttggtcggga tgaacagcgt aattatggat ggcgcggtca ttggcgaaga gagcattgtt
      781 gccgcaatga gctttgtcaa agcaggcttt agtggcgaga aacgccagtt gttgatgggt
      841 acgccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat
      901 accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacgcagcca
      961 ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa
     1021 cggtgatctt gccggatgcg acctggttgc tggataagat gctacaacat cgtatctggc
     1081 aagcaggacg cccggtatca acgagataaa attaacgacg catactcttt gacaacatca
     1141 tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggtgtct
     1201 ttcttacgcc gactaatttt tcccgactgt tattgcgcag gcgcgcgtaa atttgctcat
     1261 cgatatcgac cactttaacc agtcgctgac actggcatcc ccgcccttcc agcttattag
     1321 ggatcatctt aacttcgcag cttatttctg tgacttccga cagaatataa gtcagggtat
     1381 taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc
     1441 gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt
     1501 cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa
     1561 ttaaacaagg ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata
     1621 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
     1681 atgggcaaaa aaaaacggtc ctcatcagag aaccgtatta gttattcgat caaattagcg
     1741 gatgaaaaat atccgccacg acacgctatt atttgatctg tgcgtgcatt tcctgcaccg
     1801 aaattacttt ttcagtcgca tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca
     1861 gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg cgggagtctt
     1921 caatcgcacg acggcctgag gtggtgttga tgatgtaggt atattcgcca ttcttgatac
     1981 ggtcctgaat gtgcggacgg ccttcatgca ccttgtttac cagacgcgga ttgatacccg
     2041 cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca
     2101 gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa agcagcgcac
     2161 gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcgaac gcttcagcga
     2221 aggtgcggcc cacgcccatg acttccccgg tagagcgcat ttctggccct aacagcgggt
     2281 caacgcctgg gaatttattg aacggcagca ccacttcttt caccgagtag tacggcggaa
     2341 taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca cgcgccgcca
     2401 cttttgccag cggtacgccg gtggctttgg agacgaacgg aacagtacgc gccgcacgcg
     2461 ggtttacttc aatcaggtaa acttcgttgt ttttcaccgc aaactgcacg ttcatcagac
     2521 cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca tcctgaattt
     2581 cctgacttaa ggtgtacgct ggcagcgaac atgcggagtc accggagtgc acacccgcct
     2641 gctcgatgtg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag atggcatcca
     2701 cgtcaacttc tactgcgtca tcaaggaaat gatccagcaa cactggcgca tcgttagaca
     2761 cgctaaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg atttccatcg
     2821 cccgaccgcc gagaacgtaa gacggacgca ccaccagcgg gtagccaatc tcttttgcct
     2881 tctcaactgc catttcaata gcagtaacgg tggcgttcgc cggttgtttc agtttcagac
     2941 ggtcaaccgc atgctggaag cgttcacggt cttccgcacg gtcgatagca tccgggctgg
     3001 tgccgataac cggtacgcca gcggcttcca gtgcacgcgc cagtttcagc ggggtctgac
     3061 cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttcc agcacatctt
     3121 ccagagttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggta gagacggttt
     3181 ccgggttaca gttaaccata atggtttcgt aaccgtcttc acgcagcgcc agcgaggcgt
     3241 gtacgcagca gtagtcgaat tcgataccct gaccgatacg gtttggaccg ccgccaagca
     3301 ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca taagtggagt
     3361 acatgtaagc ggtgtcggtg gcaaactctg ccgcacaggt atccacgcgc ttgtagaccg
     3421 ggtgcaggtc atattggtca cgcagcttac ggatttccgc ttcgcgtacg cccgccagtt
     3481 ttgccaagcg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat tcagcgttca
     3541 ggccagtgat gcccacttcc gccactttct cttccagacg caccagttct tcaatctgta
     3601 ccaggaacca gcggtcaatg ttagtcaggt tgaagacgcc gtccacagac aggcccgcgc
     3661 ggaaagcatc ggcgatgtac cagatacgct ctgcgcctgc gtctttcagt tcgcgacgga
     3721 ttttggttaa cgcttccggg tcatccaggc tcactttcgg gtcgaagcca gttgcgccca
     3781 cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca atcgccatca
     3841 cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccggcg aatttttcga
     3901 agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag gaggccggag
     3961 tacgtccgcc agtgatgtcg ttcatcagtt cgtctagggt gtaacccacc gccagtttcg
     4021 ccgccacttt ag
//
LOCUS       DADLCQ010000228         3944 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000228 DADLCQ010000000
VERSION     DADLCQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.231"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..356
                     /locus_tag="JQG95_004040"
     CDS             <1..356
                     /locus_tag="JQG95_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5890404.1"
                     /translation="FVHTKPAYRSDGIKTAAVRKMRTDSNKAWKGALKRAGISNFRFH
                     DLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNG
                     TNTAQEENVVYLNAR"
     gene            618..980
                     /locus_tag="JQG95_004041"
     CDS             618..980
                     /locus_tag="JQG95_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY5890405.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYTAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            977..1897
                     /locus_tag="JQG95_004042"
     CDS             977..1897
                     /locus_tag="JQG95_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY5890406.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGAEMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPKYILKKGRK"
     gene            1900..2851
                     /locus_tag="JQG95_004043"
                     /pseudo
     CDS             1900..2851
                     /locus_tag="JQG95_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
     gene            2878..3118
                     /locus_tag="JQG95_004044"
                     /pseudo
     CDS             2878..3118
                     /locus_tag="JQG95_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060423.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gtrI domain protein"
     gene            3115..3420
                     /locus_tag="JQG95_004045"
     CDS             3115..3420
                     /locus_tag="JQG95_004045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890407.1"
                     /translation="MTVNWLSGGDIQITPVFFNADSGKINFTGVRQQTLASWHSKEAF
                     NSAPERQFIAVSIANEPERCKEMTSCLAGIQEQLGKPDEVLNFEGRVILVFNKKLNL"
     gene            complement(3654..3926)
                     /locus_tag="JQG95_004046"
     CDS             complement(3654..3926)
                     /locus_tag="JQG95_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5890408.1"
                     /translation="MITCGENGHPVIVDRPSPTPEQLALINNEKKSALIAEATNVIAP
                     LQDAVDLDMATDDETKLLLAWKKYRALLMRVDIKNTEWPKKPESNL"
BASE COUNT         1143 a          772 c          764 g         1265 t
ORIGIN      
        1 tgtttgtcca caccaaacct gcctaccgaa gcgacggaat aaaaacagca gcggtaagga
       61 agatgagaac cgacagcaac aaggcatgga agggagcgtt aaagcgggca ggcattagca
      121 acttccgctt ccatgacctg aggcatacct gggcaagctg gctggttcag tccggtgtct
      181 ctcttcttgc gcttaaagag atgggaggat gggaaactct cgaaatggtt caaagatacg
      241 cccacctttc agccgggcat ctcaccgagc acgcgagcaa aatcgatgcg attataagtc
      301 gcaatggcac aaatacggca caagaggaga acgtagttta cttaaatgcg aggtaactta
      361 ttgatttaaa tggtgccgat aataggagtc gaacctacga ccttcgcatt acgaattata
      421 agaatccgct tgtaattcaa aggattatcc catcaacact gcgctcacac gtcccaccac
      481 atcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtctc
      541 atcagacata gcaagtatcg atcaattgag acttggatga tagacttcat gcctttcaga
      601 gctcattgat taaataaatg ttaaagttat ttgtaaagta cacatctata ggtgtgctga
      661 acacacttat acactgggtg gtttttggtg tatgtatcta taccgcacat accagtcagg
      721 ctctggcaaa cttcacaggt ttcgtagtgg ctgtgagctt tagcttcttc gcgaatgcaa
      781 gattcacatt caaagcatcg actacagcga tgcgctacat gttatacgtg ggatttatgg
      841 gaatattgag tgtgattgtt ggttgggcag ctgataaatg ctcacttcct ccaatagtca
      901 ctcttatcac cttctccgcc atcagtctgg tgtgcggttt cgtctattca aagttcattg
      961 tctttaggga tgcgaaatga aaatatctct tgtcgttcct gtcttcaatg aagaagaagc
     1021 gatacctgtt ttctataaaa cggtacgtga attccaagag ttgaagccat atgaagtaga
     1081 aattgtattc ataaatgacg gaagtaaaga tgccacagag tcaattatta acgcgctggc
     1141 tgtttcagac ccgctagttg ttccgctgtc atttacacgc aactttggta aagaaccagc
     1201 cttatttgca gggttagacc atgcaagcgg cgatgctgta attcctattg atgtcgacct
     1261 gcaagaccca attgaggtta tccctcatct tattgaaaag tggcaggcag gtgctgagat
     1321 ggtgcttgct aaacgttcag accgctcaac tgatggacga ctgaaacgta agacagctga
     1381 gtggttctat aaattacaca acaaaataag caccccaaag atcgaggaaa atgtcggaga
     1441 ttttcgactc atgtctcgtg aggttgtgga gaacattaaa ctgttgcctg agcgcaatct
     1501 tttcatgaaa ggcatactga gctgggtggg tggtcagacg gatgtcgttg aatatgtacg
     1561 cgcagagcgt gttgctggca tctcaaaatt taatggctgg aaattatgga atctggcact
     1621 ggaaggtatc acaagttttt caacctttcc tcttcgcgta tggacttata taggcttgtt
     1681 tgttgcaagc atttcatttt tatatggtgc atggatgatt atagacaccc ttgtctttgg
     1741 taacccagta cgcgggtatc cctccctgct tgtatcaata cttttcttgg gtggagtgca
     1801 actgatcggg attggtgttc tcggtgaata tataggtaga atttacttag aaacaaaatc
     1861 aagacctaag tatattttga agaaaggtag aaaataacca tgagcatatg tattaaacaa
     1921 tcagcattaa aaatattatt ggctttatca gcactcctaa taacttggtt gactaccaga
     1981 tactttccag ttgaaccgga tgttgctaac agcccaattg tatggaggca tattttagag
     2041 aatggtatat catcaatcca cgactggaaa ccaaccgtgg ataattggta tttcaccgtt
     2101 tacccaatta actttttatt ttatatgctg ttaggtgatg atggcttagt agcattaaga
     2161 ttatcaactg cagttttctc tattgctata gtaatagcag cgatgttaac gctacgtaag
     2221 gcattcgggt ttacccctgc tttattttct attatacttc tatctctaat accatatttt
     2281 tcatacactt atggttttgt atcacatcct ttttctcata attcaacaaa cgctttcggc
     2341 tttttatgct tgcttatatc tgttttcaac atacagtata aaaatatatt tatcactctc
     2401 ctcttaagtt tgacagcatt attttccagc gtatccgatc cgtggtttac agctgcattt
     2461 tttattccac tattaatatc ttacttttta ttctctgtat gggataaaaa attatttaag
     2521 catactgcat taatactatt tgcatgttta atctctcttt ccaatgtatt gcaaaatcta
     2581 cttaacatac ctccccacca atttgaaatt gtatcactca atgatatgat tttaaatgca
     2641 aaatggtgca ttctgttaat aggaaaaagt cttaatttat tagttgtcga caataacgca
     2701 acatcttacg cctcatttgt catttggttc attgccatta ttacatctgc gtggtttgtt
     2761 ttatcggaca ataaaaaaaa cacataccgt atttatattg tgttattttc attattatct
     2821 atcgcaggta ttgtctcatc attcatactg agctataaat cccctgatta tattagcatg
     2881 cgtttcttta tgaatgttac ctgtttcgca ctgatattgt gctgtatcgg tacatcaaca
     2941 aaagcaaaga tattatttta tctaatcgca tttttatttt cgatcagttc aattaaatct
     3001 tacacaaata atgcttcacc attacacgat caggagaaga ttgttaaatc atacattgat
     3061 tttttaaaaa aaaataatct ccattatggg tacggctctt tttgggattt gtcaatgact
     3121 gtaaattggt tatctggtgg ggacatacag ataactcctg ttttctttaa tgctgattcg
     3181 gggaaaatta actttactgg cgtgagacaa cagacactgg cttcttggca tagcaaggag
     3241 gcgttcaatt ctgctcctga gcgacagttt atagcagttt ccatagctaa cgaacctgaa
     3301 cgatgtaaag aaatgacatc ctgccttgca ggtattcaag aacaattagg taagcctgac
     3361 gaagttttga actttgaagg aagagtaata ttagtattta acaaaaaact taacctatag
     3421 taaattgccc cctaccatat aaaccacact aacgaatgaa taacccgtct ttttcgacgg
     3481 gttatatccc tatcaggaat aaaaatggag tggttgccgt atacaacaaa ccctcattta
     3541 gagctatatt ctagagtata tgagatacta taaagttgaa ggagctatca ttagaaaaac
     3601 catatgctgt tataattaga tgtctcgctt ttcatccaat aacgaatttt ctcttataga
     3661 ttgctctctg gttttttcgg ccactctgta ttttttatat caacacgcat caatagtgcg
     3721 cgatattttt tccatgccag taacagtttc gtttcatcat ctgttgccat atctaaatca
     3781 accgcatcct gaagcggcgc tattacattc gttgcctctg ctatcagtgc agatttcttt
     3841 tcattattta ttaaggctaa ttgttctggt gttggagaag gacggtcaac aatgacaggg
     3901 tgaccattct caccacaggt aatcattttt gttacactct gcga
//
LOCUS       DADLCQ010000229         3827 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000229 DADLCQ010000000
VERSION     DADLCQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.232"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            458..2110
                     /locus_tag="JQG95_004047"
     CDS             458..2110
                     /locus_tag="JQG95_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY5890409.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDPNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2121..2291)
                     /locus_tag="JQG95_004048"
                     /pseudo
     CDS             complement(2121..2291)
                     /locus_tag="JQG95_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2370..2483)
                     /locus_tag="JQG95_004049"
                     /pseudo
     CDS             complement(2370..2483)
                     /locus_tag="JQG95_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2617..2943)
                     /locus_tag="JQG95_004050"
     CDS             complement(2617..2943)
                     /locus_tag="JQG95_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY5890410.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(3008..3799)
                     /gene="ltrA"
                     /locus_tag="JQG95_004051"
                     /pseudo
     CDS             complement(3008..3799)
                     /gene="ltrA"
                     /locus_tag="JQG95_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1056 a          880 c          884 g         1007 t
ORIGIN      
        1 acgtgggtgg actggtataa taatcgacga ttgctggaaa ggctgggcca catccctccg
       61 gcagaagcag aaaaagctta ttatgcttct atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acattctcca ggaaacttgg ggcggttcac gtcagtatca gaccatatta
      181 attgctgaaa ctgataaaca tcaaacctta tagaagggtg aatgctgaaa gggggctatg
      241 gtgatcgacg tgatgacaac gacaggtaag accgtgacct gataaacctg aattgtgtcc
      301 tagacataaa gtccgtgaag ggaataaggc tcaggtcagc gaatcttata gtggcccgca
      361 gctctgactg caataaggcc ggatacagag cagcaactac tcgtcgatgt cagaacaacg
      421 gaagccttgc aaacggggat gcgttcatac aggtacaatg ataccgttaa aaaaaaacat
      481 tacgctaata atgttcaccc tgtcattact gacaggaaat cctgctatag cgtatgaaac
      541 agataaagta tataaaataa ccgttctgca tacaaatgac caccatggac acttttggcg
      601 taacaatcat ggtgagtacg ggttatcctc ccaaaaaact ctggtggaca atatccggca
      661 gaaagtaata aataatggcg gaagtgttct attattatct ggtggtgata ttaacaccgg
      721 tgtacctgaa tcagaccttc agaaggctga acccgatatt cgtggaatga atctaattgg
      781 ttatgatgca atggccgtcg gtaatcatga atttgataat cctctaaata tattacgtca
      841 gcaggaaaaa tgggccacat ttcctttcct ttctgctaac atatatcaaa aaagtacagg
      901 gaggcgttta ttcagtccat ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat
      961 tggcttgacg acagatgata ctgcgaaaac aggaaattct gaatatttta ctgatattga
     1021 gttccgtcaa cctgctgctg aggcgaggag tgtcattgat gaacttaatc agcaggaaaa
     1081 gccagatatt attattgcgg caacacatat gggtcactat gacaatggcg agtcaggttc
     1141 gaatgctcct ggtgatgttg aaatggctcg cagtttgccg actggctcat tagcaatgat
     1201 tgtgggagga cattcacaag ctcctgtctg catggccagc gataataaaa aacagtggaa
     1261 ttatattccg gggacaacct gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc
     1321 gcatgagtgg ggaaaatatg tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa
     1381 actggttaat tatcagttac acccagttaa tctcaaaatg agaattacca gagaagatgg
     1441 aaaaacggaa ttttcttttt acacccccga aattactgaa gatccacaga tgttatcctt
     1501 actgacacca ttccagaata aaggcaaagc acaactggat gtgaaggttg gggttgtgaa
     1561 cggacgtctg gaaggggaca gaagtaaagt tcgttttgtt cagaccagta tgggccatct
     1621 gattctgagc gctctgacag aacgtattga cgcagatttt gctgttgtca gtggtgggga
     1681 aattcgagat tccatagaga gtggtaacat cacatacaaa gatatactga aagttcagcc
     1741 atttggtaat acggtagtaa gtattgattt gacggggaaa gaagtagctg actacctagc
     1801 tacagtggca cagatgaaac cggattccgg agcctaccca caatttttga ataccagctt
     1861 tgtagtcaaa aaaggaaaaa tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccctaa
     1921 caaaaaatat cgtatgacaa catttagctt taatgctact ggtggggatg gctatccacg
     1981 tattgataat agacccggat acataaatac cggatttatt gatgctgaag tacttatcga
     2041 atatatccgg aaacattcac cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag
     2101 ttggcagtga taacgtaatt gacgatcatc acgtgttttt tgctgacagt cgctgtgact
     2161 ttgtgaaagt agtctctgcg gatgtttgcg atatgggaat gcagttgctg tattttgtgt
     2221 ttctgcttct gccagttgtt gctgaattta accttgcggc ttaactgatg ttggagcgct
     2281 gccagcttct tcgtcaattt cgctgattaa agtttattca ttatggcatg atctgcgtaa
     2341 gcggctcgtc agaaccatat tgatatttac taagagctca gatcaacttt ccagggcaac
     2401 agatcgcgta cccggtttgc cggtcagtcc tggatatgct caatgacgta acgcagccat
     2461 ttttctggct ccacattgtt cagacggcat gtgccgatca gcgagtacaa caccgccgca
     2521 tgttcgccac cgctatcgga accccgccgg agagagtttc actctccggc tgctcacgga
     2581 accgtacgtg acactctcgc gtcatacggc tcttgatcac tcattcgatg cacgtagtaa
     2641 ataccagtgg gcaaatagct tcggatactg tttccttacc agcgccagct ttcgctgagc
     2701 cttccggttc gaaagtctgt atttagactg catccactta agcaaacgtg actgcattgc
     2761 actccacagt cgatagctga agtttctgga ccagaacttt ccatagtact caatccagcc
     2821 tctcactatc gcattgtagc gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg
     2881 tatacgccac ttcttgattg tttctgttat tttgcacatt gctgcatttg atgcacccgg
     2941 catacatttt ctgtacaatt cgcctttgaa attcttcaga gtacgcactt tgaagtcata
     3001 tccgagagtg gcatacaaca tcatcggcgt acctttcgaa gggaacgccg ggaaactgac
     3061 gttgcatcca catatcaaag gcatagtgca ggagcagatt tgagagcagg ggggagatta
     3121 cgccgccctg cggcgttcct cgaccccgtg ccagcattgc tccatccggc atttgcaccg
     3181 gtgccttcag cctgcgctca atgtacatca ccacccagcg tgcctgagta tgatgctgca
     3241 ctgtcttcag tagcaggtca tgatcgatat catcaaagaa gcctttaata tcgacttcca
     3301 ccacccagtc aaacttccag cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt
     3361 tacccggtcg ataaccatac gagtctgcat ggaacagggg ttccagtacc ggctcgagta
     3421 tctgcttaac gaccatctgg gcgatccggt ccgacacggc cggaataccg agcggacgta
     3481 caccaccgtc agctttggga atgtccacac gccttaccgc ctgcggcata taactgccgg
     3541 aagccattct gttccacagt ttgtagagat tgtctgtaac attctcgtca aacccggcca
     3601 gtgtctgccc gtcaatgcct gccgccccac ggttggcttt tactcgttta taagcttccc
     3661 atacctgtcg tttacttaca ggaaatggct ttgccgattt tacagcagtc ctcccatcgg
     3721 cattgtcgcc cgtggttgcc gccgcaccat cagcacaatg gggcaagccc tttgctccgg
     3781 acccattacg gtccttcacc gctactacgg tttactccgt ccctgta
//
LOCUS       DADLCQ010000230         3790 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000230 DADLCQ010000000
VERSION     DADLCQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.233"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(452..824)
                     /locus_tag="JQG95_004052"
                     /pseudo
     CDS             complement(452..824)
                     /locus_tag="JQG95_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            827..1024
                     /locus_tag="JQG95_004053"
                     /pseudo
     CDS             827..1024
                     /locus_tag="JQG95_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1068..1187
                     /locus_tag="JQG95_004054"
                     /pseudo
     CDS             1068..1187
                     /locus_tag="JQG95_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1437..2303
                     /gene="ospB"
                     /locus_tag="JQG95_004055"
     CDS             1437..2303
                     /gene="ospB"
                     /locus_tag="JQG95_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY5890411.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2632..3372
                     /locus_tag="JQG95_004056"
     CDS             2632..3372
                     /locus_tag="JQG95_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY5890412.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1161 a          745 c          761 g         1123 t
ORIGIN      
        1 ggggaaaaac gtgtattttt agtcattctg tttacctctt tctcagggcg tttagtctcc
       61 aggatttccg gggcggttca aaaaactctt cgttgattac tgtggactta ctgttggcat
      121 taccgaccct gagaccggag aaataagaac cgcttagatc atcgtggctg ttctcggggt
      181 atcaagttac acatgggcag aggctaccca aggtctacta ttatcgcctg aaagcgctga
      241 tggaacagtg ctatcaggga catgctgatg aaaataccta aataatcagc cagcactcta
      301 tctttccaaa tccacagcat agcaaagaga gcaaaagagc ctgtaaattc agaaattaag
      361 gaaacgcctc ttggtgtaca taatctatct tatgttaaat gaaatctaat ttcttgatta
      421 tatgctggat cgttcgtggg tggctttgcc cctatatttc cagacacctg ttatcattgg
      481 cccgctgccg cagatattcc cgtggcgagc gataatccag cgcactatgc ggatgccatt
      541 cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc cgttaacccg tcaggtttgg
      601 gcatgatact gatgtagtca tactttattg ttttcacgaa gctctttgct attctgttac
      661 tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa gtcccaacat ccgggtgaac
      721 tggtgtgttt cattagctcg gtagcatgaa ccattatccg tcagtcactt cactggagac
      781 gccggaagct cgttgccgaa gcggcgttcc accgctccca gcatgtcgcc aacgggagag
      841 catctacggt ctgattgaac tgtttattca aattgtccac cgtctgtcag tacgctctga
      901 acgtcggctg gttaaaacac tgctcgctga ttttcagagg gttcacggta aaacagcact
      961 tttattccgg attgcggaag cggctctgaa taatccggat gggctggtta aagaggttgt
     1021 gtaccatctg catattgttg aaccagatcg cagcggtaag agaagttcat catacttggc
     1081 gcagttacgc gatgtttctg ctagaggtga tgccgtaaag aacggcagaa cattaccgga
     1141 acaagatagc ggtctacctg cactggtatc agacccgggg cttccccgga tgatctccac
     1201 ggtactttag gtactgtgtc tatattatgt aggtaggatc aattaacttt gtagttgaaa
     1261 ttctttcatt gaaataatga ataaaaaaga caaaccgcat taaacaatgc cagaatataa
     1321 ttgcattttt gttccgtttt tttaaatcat agtatacaaa gaagcttgca tactatatga
     1381 aaatataaca ccctttacta tgatgtttat aaaacaatat atggagtcat gtaggtatga
     1441 atttagatgg tgttagacca tactgtagaa tagtcaataa aaagaatgaa agcatatcag
     1501 atattgcatt tgcacatata ataaaaaggg taaaaaattc atcatgtact cacccaaaag
     1561 cagcattggt ttttttagga gagaaaggtt tttgtgatag caatgatgtt ctatctatta
     1621 tgggacaaca aataccaaga gtatttaaga acaagatgtt atatgattat gtttttaaaa
     1681 atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc atggctacca cagagtgaac
     1741 caatagtaat aaataatgat gatgacgcat tgaatgctgc tgcttatttt tctgtaaaaa
     1801 aagcgaaaat aaaaacagta aacgatactg attttaaaga gtataataag gtttatattc
     1861 ttgggcacgg tagtcctggt tctcatcaat taggccttgg ttcggaactt attgatgtac
     1921 aaacaatcat ttcaagaatg aaagactgtg gtattctaaa tgtgaaagat atccgtttta
     1981 cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt taacaatgcc cctgctgaaa
     2041 gtctttcttg tatccttaac tctctgcctt tttttaagga aaaagaatct ttgctagagc
     2101 agataaaaaa acaccttgaa aacgatgagt cattgagtga tggtctaaaa atatccggct
     2161 atcatggata tggagttcac tatggtcaag agctttttcc ctactcacat tatcgttcaa
     2221 cttcaattcc tgctgatccg gagcatacag taaaaagaag ctctcagaaa aagactttta
     2281 ttattaataa agaactggat tagtataaaa tttttaacct atagttaatt gtttgcattt
     2341 aagatgaagt tattttatct ttagtagaaa agtgcttccc tatgagggaa aatcacaaat
     2401 catcataata cagagacaaa acgatacgaa aaaatagata aaaaacatca ttcttttacc
     2461 acattatttt ccgtgaatat gaaaaataat gttattactt taatataaga ctattttttg
     2521 tttttccatc actctgttca aatttttccg catgacttgt gttttttgta atacagctcg
     2581 ttttttacag ctgaccaaaa tcatcaatta attatgctaa ggaaataaat tatgaaaacc
     2641 aaaaactttc ttcttttttg tattgctaca aatatgattt ttatcccctc agcaaatgct
     2701 ctgaaggcag aaggttttct cactcaacaa acttcaccag acagtttgtc aatacttccg
     2761 ccgcctccgg cagaggattc agtagtattt caggctgaca aagctcatta tgaattcggc
     2821 cgctcgctcc gggatgctaa tcgtgtacgt ctcgctagcg aagatgcata ctacgagaat
     2881 tttggtcttg cattttcaga tgcttatggc atggatattt caagggaaaa taccccaatc
     2941 ttatatcagt tgttaacaca agtactacag gatagccatg attacgccgt gcgtaacgcc
     3001 aaagaatatt ataaaagagt tcgtccattc gttatttata aagacgcaac ctgtacacct
     3061 gataaagatg agaaaatggc tatcactggc tcttatccct ctggtcatgc atcctttggt
     3121 tgggcagtag cactgatact tgcggagatt aatcctcaac gtaaagcgga aatacttcga
     3181 cgtggatatg agtttggaga aagtcgggtc atctgcggtg cgcattggca aagcgatgta
     3241 gaggctgggc gtttaatggg agcatcggtt gttgcagtac ttcataatac acctgaattt
     3301 accaaaagcc ttagcgaagc caaaaaagag tttgaagaat taaatactcc taccaatgaa
     3361 ctgaccccat aaagctggac agcctgtatc aggctatgga gggcccatag acaaatctac
     3421 cctatatgag cataggagga gtctatgggc acaccacgtt ttacccctga atttaaggga
     3481 ttactggaaa ggctgggaca tatccctccg gcagaagcag aaaaagctta ttatgctgcc
     3541 atcggaaacg atgatctggc aacctgagtt cacagataaa acattctcta ggaaactcgg
     3601 ggcggttccg ttcaccacat gcaatgtggt gttgcagggg aacggtctgc accatcccct
     3661 atgtcgatca atataacaga aatgacaact tcagattcag ggcacaacct aaatatattt
     3721 taggtcacct ctcaaatcgt ttgcctgata ccgctccatt ttttaataaa aaaatcaatc
     3781 atttttgaat
//
LOCUS       DADLCQ010000231         3711 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000231 DADLCQ010000000
VERSION     DADLCQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.234"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            172..1275
                     /gene="proB"
                     /locus_tag="JQG95_004057"
     CDS             172..1275
                     /gene="proB"
                     /locus_tag="JQG95_004057"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY5890413.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1287..2540
                     /gene="proA"
                     /locus_tag="JQG95_004058"
     CDS             1287..2540
                     /gene="proA"
                     /locus_tag="JQG95_004058"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY5890414.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2655..2730
                     /locus_tag="JQG95_004059"
     tRNA            2655..2730
                     /locus_tag="JQG95_004059"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Thr,seq:cgt)
     gene            complement(2745..>3711)
                     /locus_tag="JQG95_004060"
     CDS             complement(2745..>3711)
                     /locus_tag="JQG95_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5890415.1"
                     /translation="LEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKM
                     TNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLD
                     KAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQ
                     IDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTK
                     APTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWES
                     IEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
BASE COUNT          851 a          933 c          993 g          934 t
ORIGIN      
        1 aatgtggtaa tttattaaat ctgtaataaa agcgtaaaca actgccgcta cgcttgctga
       61 tcccgcgcaa caaaacgcca tgctttgctc gcagatggtt ggcaaccgac gacagtcctg
      121 ctaaaacgtt cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac
      181 agccagacgc tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgtctg
      241 aaccgtgccc atatcgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat
      301 cggattgtta ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg
      361 gaactgccag cgactatcgc ctcgaaacaa ctgctggcgg cggtagggca gagtcgactg
      421 attcaactgt gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg
      481 acccgtgctg atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg
      541 ttgctcgata acaatatcgt tccggtaatc aatgagaacg atgctgtcgc tacggcagag
      601 attaaggtcg gcgataacga taacctttct gcgctggcgg cgattctggc gggtgccgat
      661 aaactgttgc tgctgaccga tcaaaaaggt ttgtataccg ctgatccgcg cagaaatccg
      721 caggcagaac tgattaaaga tgtttacggc attgatgacg cactgcgcgc gattgccggt
      781 gacagcgttt caggcctcgg aactggcggc atgagtacta aattgcaggc cgctgacgtg
      841 gcttgccgtg cgggtatcga caccattatt gccgcgggca gcaagccggg cgttattggt
      901 gatgtgatgg aaggcatttc cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa
      961 aaccgtaaac gctggatttt cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg
     1021 gcaactgccg ccattctgga acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg
     1081 actggcaatt tctcgcgtgg tgaagtcatc cgcatttgca acctcgaagg tcgcgatatc
     1141 gcccacggtg tcagtcgtta caacagcgat gcattacgcc gtattgccgg acaccactcg
     1201 caagaaattg atgcaatact gggatatgaa tacggcccgg ttgccgttca ccgtgatgac
     1261 atgattaccc gttaaggagc tggccgatgc tggaacaaat gggcattgcc gcgaagcaag
     1321 cctcgtataa attagcgcaa ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg
     1381 ccgatgaact ggaagcacaa agcgaaatca tcctcaacgc taacgcccag gatgttgctg
     1441 acgcgcgagc caatggcctt agcgaagcga tgcttgaccg tctggcactg acgcccgcac
     1501 ggctgaaagg cattgccgac gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc
     1561 aggtaatcga tggcggcgta ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc
     1621 tgggggttat tggcgtgatt tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc
     1681 tgtgcctgaa aaccggtaat gcggtgatcc tgcgtggtgg taaagaaacc tgtcgcacta
     1741 acgcggcaac ggtggcggtg attcaggacg ccctgaaatc ctgtggctta ccggcgggtg
     1801 ccgtgcaggc gattgataat cctgaccgtg cgctggtcag tgaaatgctg cgtatggata
     1861 aatacatcga catgctgatc ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac
     1921 agtcgacaat cccggtgatc acaggtggta taggcgtatg ccatatttac gttgatgaaa
     1981 gtgcagagat cgctgaagca ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca
     2041 catgtaatac ggttgaaacg ttgctggtga ataaaaacat cgccgatagc ttcctgcccg
     2101 cattaagcaa acaaatggcg gaaagcggcg tgacattaca cgcagatgca gctgcgctga
     2161 cacagttgca ggcaggccct gcgaaggtgg tggcggttaa agccgaagag tatgacgatg
     2221 agtttctgtc attagatttg aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc
     2281 atattcgtga acacggcaca caacactccg atgcgatcct gacccgcgat atgcgcaacg
     2341 cccagcgttt tgttaacgaa gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt
     2401 ttaccgacgg cggccagttt ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc
     2461 acgcgcgtgg cccaatgggg ctggaagcac tgaccactta caagtggatc ggcattggtg
     2521 attacaccat tcgtgcgtaa ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa
     2581 ggccattgac gcatcgcccg gttagtttta accttgtcca ccgtgattca cgttcgtgaa
     2641 catgtccttt cagggccgat atagctcagt tggtagagca gcgcattcgt aatgcgaagg
     2701 tcgtaggttc gactcctatt atcggcacca ttaaaatcaa taagttacac atcattagta
     2761 ccttccttat tttttgactg ggacaaattt gggaccgatg ggttcaggat cgagtctatt
     2821 tgccgtgcgt gttcggtaag gtgattaggt gcaaggtgag catatcgacg aaccatttcg
     2881 atagactccc agcctcccat ttcctgtaac actgacaacg ggactccggc ttgaaccagc
     2941 caacttgccc aggtgtgtct caagtcgtga aatctgaaat catcaatacc agcccgtctc
     3001 agcgccgctt tccaggctgt gtttgcgtca taccgcatct tccttactgt tggcgctttc
     3061 gttccgtctg gtttggtaca gctttccttg tacacaaata cccaacggtg atgattcccg
     3121 atttgttttt tcaatacgcg acatgcagta tcattcagcg caacgccaat tgcgcggttt
     3181 gatttactct cttccgggtt tatccatgcc acccggcgct gcatatctat ttgttgccat
     3241 tcaaggttga tgatgttcga gcgtcttaag cctgttgcca gtgcaaattc aacaacagac
     3301 tttaatggct ccggacattc atcaatcagc ctttgtgctt catggggctc cagccagcgg
     3361 atccgtttat tctttggttg aggcacttta ataattggtg ccttatccag cattttccat
     3421 tcacgctctg cggctcttag tagggccttt ataaatgaaa gatgcgtagc cttcgttgca
     3481 acggacgctg gttttggcgt gtattctgga acaggtttcc ctttttttct gcatgcttct
     3541 gccctgagtt tccagttttc ctcatgacgc cggttcgtca ttttctgcat tgctgaataa
     3601 atttttgatt cagtgatgtc tcttagttgc atccctgcga aatgttgaag ccagaatccg
     3661 atccggcttt tgtcatcgtc cagtgatttc ttatgtgctt tctcttcgag c
//
LOCUS       DADLCQ010000232         3590 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000232 DADLCQ010000000
VERSION     DADLCQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.235"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            32..493
                     /locus_tag="JQG95_004061"
     CDS             32..493
                     /locus_tag="JQG95_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY5890416.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..1004)
                     /locus_tag="JQG95_004062"
     CDS             complement(534..1004)
                     /locus_tag="JQG95_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY5890417.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="JQG95_004063"
     CDS             complement(1051..1770)
                     /gene="yehT"
                     /locus_tag="JQG95_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY5890418.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="JQG95_004064"
     CDS             complement(1767..3452)
                     /gene="btsS"
                     /locus_tag="JQG95_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY5890419.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          953 a          926 c          817 g          894 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctgacgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      721 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      781 gcagactcat ccttgccgcg tttttcataa atcaggccat tgaggaatga cgacaaaaca
      841 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      901 tccgcggtgg cttcgacatt atccagcgcc agaatacgca ccaggtcatt attattggct
      961 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta tcatgaggcc ttcaggtgtt
     1021 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
     1081 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
     1141 gttatcttcc agacgaatct cctgtaaatg cgcgaggtta accagatact gacgatggca
     1201 gcgcagtagt ggtgtacgac tttccaaggt acgtaatgtc aattcggtaa agccctcttt
     1261 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
     1321 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
     1381 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
     1441 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaggcat gttcttcaaa
     1501 ggctttaata gcgtattcgt caaacgcagt gagaaaaaca atatacgggc gatgttccgg
     1561 gtcaagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1621 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1681 aacgatttca atatcgctct gctcctgcaa aaatatacgc aggttctccc gtgctaacgg
     1741 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1801 attcgggtgt aactatcagg ctcacaggcg acgcttattc catagtcatc gccaaaccgt
     1861 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1921 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1981 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     2041 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     2101 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     2161 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     2221 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     2281 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     2341 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     2401 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa ccggtttttg
     2461 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     2521 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2581 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2641 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2701 gtaatcgcga ctgcaccaat atccagctcc tgatacagta cctgagccac tttcatgctg
     2761 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2821 gtggcagaaa aagccgaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2881 ataaacagcg ccgcgccgac ggtattggtg accatcattg gcgcagcaat attactcacc
     2941 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     3001 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     3061 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     3121 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     3181 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     3241 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     3301 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     3361 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     3421 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     3481 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3541 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag
//
LOCUS       DADLCQ010000233         3548 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000233 DADLCQ010000000
VERSION     DADLCQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.236"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..465
                     /locus_tag="JQG95_004065"
     CDS             <1..465
                     /locus_tag="JQG95_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5890420.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /locus_tag="JQG95_004066"
     CDS             802..2415
                     /locus_tag="JQG95_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5890421.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQG95_004067"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="JQG95_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY5890422.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DADLCQ010000234         3469 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000234 DADLCQ010000000
VERSION     DADLCQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.237"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..206
                     /locus_tag="JQG95_004068"
     CDS             <1..206
                     /locus_tag="JQG95_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890423.1"
                     /translation="CLWSVSRVRRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRG
                     ITLLSKRRRLRYSTVGLTRYRKR"
     gene            270..953
                     /locus_tag="JQG95_004069"
     CDS             270..953
                     /locus_tag="JQG95_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY5890424.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            954..1175
                     /locus_tag="JQG95_004070"
     CDS             954..1175
                     /locus_tag="JQG95_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890425.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            1187..1621
                     /locus_tag="JQG95_004071"
                     /pseudo
     CDS             1187..1621
                     /locus_tag="JQG95_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            1666..2436
                     /locus_tag="JQG95_004072"
     CDS             1666..2436
                     /locus_tag="JQG95_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890426.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2775..>3469)
                     /locus_tag="JQG95_004073"
     CDS             complement(2775..>3469)
                     /locus_tag="JQG95_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5890427.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
BASE COUNT          758 a          936 c          975 g          800 t
ORIGIN      
        1 gctgcctgtg gtcagtgtcc cgcgtccggc gggcacggga cgggcgcttt taccgctccc
       61 ggctggcgtc ggtgacagtg tacgccagcc cgtcgccctt ttctgatgaa cgcccttcaa
      121 gccgctttcg cggcataacc ttgctgtcaa aaagacgacg gctgcggtat tccacggtcg
      181 gcctgacccg ttaccggaag cgatgaacag cccacaggca gcggggaacg ggcaccacag
      241 gggtgccctc tgtaagagaa ggatctgtta tgtcccgttt tgtcctcggt aactgcatcg
      301 atgttatggc ccgtattcct gataacgcca ttgatttcat cctcaccgat ccgccatatc
      361 tcgtcggttt tcgtgaccgt caggggcgta ccattgctgg tgataaaact gatgaatggc
      421 tgcaaccggc ctgtaatgaa atgtaccgcg tactcaaaaa agacgcgctg atggtgagct
      481 tctatggctg gaaccgcgtc gatcgcttta tgtctgcctg gaaaaatgcg ggattcagcg
      541 ttgttggtca cctggtcttc accaaaaact acacatcgaa ggccgcatat gtgggctatc
      601 gccacgaatg cgcctacatc ctggcaaaag gccgtccacg tctgccacaa aacccgctgc
      661 cggacgtgtt gggctggaaa tattcgggca accgccatca cccgacggaa aagcccgtta
      721 ccagcctgca accgctgatt gagagcttca cacacccgaa tgcgatagtg ctggacccgt
      781 ttgcaggcag cggttcaacc tgcgtcgccg ccctccagtc cggacgccgg tatatcggta
      841 tcgagctgct tgagcagtat caccgtgccg gacagcaacg actggctgcc gtacagcggg
      901 ccatgcagca gggggccgcg aatgatgact ggtttatgcc ggaggctgcg taaatgaact
      961 atgccggaca cgaaaaactt cgcgccgaag tggcggaggt ggccaatgcc atgtgcgacc
     1021 tgcgtacaac catgaatgag atggagcggc ggtacagctt taatgctgac accctgccgg
     1081 aacgtctggt gcgtcagacg ttgtttcgcg caaaccgcct cttgatggag gcatataccg
     1141 aaattctgga actggattcc tgcttcaaag attgagggga gagagtatgt acggaacatg
     1201 cgaaacgcta tgccgggcgc tggcagcaaa gtattcggga gacacgccgc tgatgctggt
     1261 tatctggtcc ccggaagaga ttcaggccct cgctgacgga atggatattt ccctgtccga
     1321 tcatgaaatc aggaccgtcc tggcgcacct ggaggacatc ccggaagact agcggattga
     1381 gtccggtatt tcttccgccg cggcgatgga gattatcagc aacgtgcgcg aaaaccgcca
     1441 ggtgaccgtc cctgctgaac tgctggagac cctgactcag atcgctgaac aggcactgtg
     1501 gaaacgtgaa tgggctgccc gggatcacgg tttccccctc ccggaatacg ttacccgccg
     1561 acaggcgatg gttgatcagg cccgcagcct gctgaaaaac aacacacacg aaaacgactg
     1621 atgttatcgc cgcctgtggg cggcgataca gggagttcat gaattatgaa tgaaacgctg
     1681 aatgctctga tttgtcgtca cgcccgtaac ctgctgctgg cgcagggctg gccggaagag
     1741 acggatgttg accaatgtaa cccgaactat ccgggctgga tcagcattta cgtgcggctg
     1801 gatgcgcccc ggctggcgac gttactcgtt aaccgccacg atggcgtgct gccgccgcac
     1861 ctggcctccg ccattcagaa actgaccgga accggagcgg aacttgttct gtccggcagt
     1921 cagtggcagt cgctgccggt acttccggcg gacggaacgc aggtgtcttt cccgtatgcc
     1981 ggtgaatggc tgaccgaaga cgaaatcagg gcagttcttg atgcggtgcg cgatgcagta
     2041 tgcagtgtca gttgccgggg ggcagaagat gcgcggcgta tccgggcggc gctgaccaca
     2101 agcggtcaga cgttgctgac ccgccagacg cgccgctttc gcctggtcgt gaaggaaagc
     2161 gatcacccct gctggctcga tgaagacgat gaaaacctgc ccgtggtgct cgatgccatc
     2221 ctgaaccggg gcgcacgttt ttcggcggtg gaaatgtatc tggtcagcga ttgtattgag
     2281 catatcctgt ccagtgggct ggcctgcgat gtgctgcgta taccggatga accgccccgc
     2341 cggtggtttg accgtggtgt tctgcgggaa gttgtccggg aagcccgggc cgaaatccgc
     2401 agcatggcgg atgccctggc aaaaatccgg aaatgatgac gatggcgggg aaaatctatg
     2461 gtcacccccg tttttgcaac actgattttt gattaatggt gggcttgctt aaatctatcc
     2521 ggcgtctctg tgggcaaggg atgcccgcgc ctcgatgagt tccgcacctg ataatcctaa
     2581 aaatcccctc ggcttcagtg agccattttc ccctgttagg tttttaatca ggtcgatttg
     2641 ccgttcatgt catcaataat caatcttcgc aaaaccagac ggtaacttgt atcttattgt
     2701 ttgcaataag ctaattgagt tttgtcagca tctatatatc aggcctgata ttctttttgc
     2761 tgagtagtga ttgcctaggc aattcgggcc agtttatttg ccagagcgca ggcaaccaca
     2821 ttcgaatgct tcttgttgag ttgctcccga acccactcag caagttttcc atgctggcgt
     2881 tcaagctgca tcatgaatga gcgggcacac tgaaccagta accggcgtaa atttttatca
     2941 cctcgcttac tgatacccaa cagagtcgat tttcctccag tgctatactg ccggggaacg
     3001 agccctgtgg aagccgcaaa atctctgctg catgaaaact gtttcccgtc accaagttgc
     3061 gacgatagta agctggcagt aatcggccca acaccaggaa ttgtcataat gcgctgagcg
     3121 gtatcatcag cgttgattga ctgacttaat tctgattcca gctctgctat ttgctcaaca
     3181 aggtaaaggt agtgggtatg caacctcacc aaaaggcggc tcagatattc ggggatttcg
     3241 tgttcagcca atacgagaga caaccgtttt attacggcat cccctgtcgg caggctgatt
     3301 ccaaattcca gcaggaaacc gtggatctgg ttggttgttt tcaccttatc tctgatgagc
     3361 gattctctga cccggtgtaa tgcccttatt gcttgctggg tctcagttct tggctggaca
     3421 aaacgcattg atggtcgtga agccgcttca catatggctt ctgcatcaa
//
LOCUS       DADLCQ010000235         3428 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000235 DADLCQ010000000
VERSION     DADLCQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.238"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..954)
                     /locus_tag="JQG95_004074"
     CDS             complement(<1..954)
                     /locus_tag="JQG95_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY5890428.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNV"
     gene            1325..2284
                     /locus_tag="JQG95_004075"
     CDS             1325..2284
                     /locus_tag="JQG95_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5890429.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            2284..2679
                     /locus_tag="JQG95_004076"
     CDS             2284..2679
                     /locus_tag="JQG95_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY5890430.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            2862..3242
                     /locus_tag="JQG95_004077"
     CDS             2862..3242
                     /locus_tag="JQG95_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY5890431.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQHITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            3288..>3428
                     /locus_tag="JQG95_004078"
     CDS             3288..>3428
                     /locus_tag="JQG95_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890432.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCS"
BASE COUNT         1084 a          809 c          730 g          805 t
ORIGIN      
        1 cacattgtcg actatccacg caggtccggt gacttccggc tgctcatcgt catctgtcgg
       61 ggaagaccat tcaagttccc cccacagcac atcgacgagc tctccacgct catacaactg
      121 ccagccacag aaatcacagc cctgttcggc ataccagtgc tccagcgtgc agctgaaacg
      181 gcggcttaat tctgcaataa cgtcgctttc cggctgacac cacggagtgt cgaaatccac
      241 ctgaatgaag ttttctccct gactgctgat attcaggtca tatccacaag gccacttcac
      301 gccataccgt tccgtatacc agtgatatgc agaaggaaca ccgttcagaa caccaccatt
      361 aaagccgttc acttcaacat ccaggcgggt cggcagaacc gtcagcaggt caaacggcat
      421 ggttttttcc ggcactacgt tgtcacacag acggttccac cacaccgata catcctcgtt
      481 accccatatg tcacaccagt cacctctttt tgcggtgaaa tgcacagtga tggtttccct
      541 ggcatcatcc ggtaatcctt cccaacgacg ctggccagta ccgctctgta accagagttc
      601 atgcagcatc aggcagtttt gttcatccag caacacaccg ttctgcaggt gtgtcagcca
      661 gcgggtgaac gcgatatttt ccgtcgatac agcaccacgt ccggcagccg tcactccggg
      721 gcactgttca gagcggatat tttcagtgat ctgcagaagt ccggcacttc cggccagaaa
      781 cagctgaata ccttcatttg tggcgcggcg ataaagcggt gtgactgcac cgctggccag
      841 tcgtttaatc tcagcgatct gtgccggttc accaggaaaa tacatacgat tcgagcacca
      901 gtttggcatg ggaattctcc tctcacatgc ggcctccatc aggccgtgcc gcacctgtaa
      961 ccgggcgacc gcatacgaat gcaggtgcgg ccccgaagcc gcagcgcgtg gaggaggaat
     1021 gacgaaacgt gaccgggtga ggaggccggc gacagcgaca cacttgcatc ggatgcagcc
     1081 cggttaacgt gccggcacgg cctggttaaa tagtatccgt ctcagttcgt ggcgcaaaat
     1141 gagaaaccaa taagaactca aaatgaacca caacaagaac ataaagagac tctaaaagag
     1201 caacaccgtt tgacatagac tcaaaatgag tcacaataag aaacaataaa aaactcaaaa
     1261 agatgcaccg acataaagga tgctcaattt gagataaaca caaactcaaa acgaggcaaa
     1321 aagaatgcta aaagtatcat gcgatgatgg ttcaactaac gtaaaactgg cctggcttga
     1381 agatggcgag gtaagaacca gtctgtcagg aaacagcttt aaagaaggct ggaacccggg
     1441 actgtttaat gccggcaaag tttacaatta cgtcgtggat gaaaaaaaat acacctatga
     1501 tttgggaagc actgctgtca taggtacaac acatgtcagt tatcaataca gcaccaccaa
     1561 tctgcttgcc atacatcatg cactgctgac atccggcctt cagccacagg atgtagaact
     1621 gacagtaaca ttacctgtga ccgaattctt tgataatgac aatcaaccaa atgaagaaag
     1681 aatagaaaga aaaaaggcaa atgttctccg tgagatatct ctgaataaag gggaaacatt
     1741 taaaattaaa aaagttaatg taatgcctga atctttgcca gctgcatttg aatcactaaa
     1801 aaaagataaa gtaaacaaac ttgaacgcag cctgattatc gatcttggag gaaccactct
     1861 ggattgtggt ttaattctcg gtgcatttga aggcatttct gaaatccgtg gctattcaga
     1921 aatcgggaca tcccgcatca ctcataccgt catgaatgcg ctgacgaaag cctccacacc
     1981 atgtaattat tttattgctg atgagttaat aaaaaacaga cacgataatg aatatctcca
     2041 gacattaata aatgatgtag ctgaaataaa aaatatttct catgtaatag acagagaagt
     2101 gaaatctctg gcagaaagca tcaggcagga aatttcaaca ttctcaggaa tgaaccgaat
     2161 ctatctcact ggcggaggag ccgagcttat ttaccctcac ataaagcaat atttcccgaa
     2221 tctgaaagtg aacaaggtgg atgaacctca gtttgcattg gtgaaagcaa tggtgcatgc
     2281 gtaatggaaa gcagtgatcc aaaaaaaaga aagaaagttg tggcatacct gcacccggca
     2341 ctttatccgc aggacaacct gacacaacaa acaatagact ctctcccagt acaaatgcgg
     2401 ggagatttct acagacaatc ccttatttgc ggagcagctc tttattctgt tgccccccga
     2461 ctgttaacgc taatcagcgt ttttttcagt gagaaaatca ctgcagaaaa tctggtgaaa
     2521 cttatagaac aaacaacagg atacacatcc acatcaattg atataagtgt gttaaaaaac
     2581 atcatagagg catcttcaga aaacaaatca gagagcatca cttcaaaaga tgatttcgaa
     2641 gaacaaacca gacgcaatct ttctatgtta aaaaaataat aaaagaagag caggttcagt
     2701 caattacctg ctcttctttt atacatacta aaaaatcaga tttttacgaa tattcagatt
     2761 atcataaaaa atgcaacaga caggaatgca ataattttgc attatctcgt tgcttgtaat
     2821 gcaaatatgc attattgtca gtgtgtgaca ataacagaga gatgacgatg gcgaccatta
     2881 acgcacgtat tgatgatgat ataaagaacc aagcagatga agtgctgaag ctgatgaata
     2941 tcagtcaaac tcaggctatt gcagcttttt atcagcatat tactgaacaa aagaaactgc
     3001 catttgtgat cacctcaatt gtaaaaacgc cgcatgattt attacgtgaa agtacagaca
     3061 tgttagctga agctcttgca gtgatttcca atctacaggt gtggactgaa cagcaggatg
     3121 ggattggaaa agcaaagcta atggagtatt accgccgcct cgacgcactg tactgttgtg
     3181 caaaagagaa aataggtctt ctttcagata accgggatgc tgaactaggc tgtgtccctt
     3241 aatcatctga gttatagtaa ctgtctgttt acacaacata ttgaactatg gctcgctacg
     3301 accttcccga tgaggcatgg accatcatca agcccctgtt acctcctgaa cccgccacac
     3361 cacgggccgg acgcccatgg gctgagcacc gtaaaatcat caatggcatg ttctgggtgt
     3421 tatgttcc
//
LOCUS       DADLCQ010000236         3411 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000236 DADLCQ010000000
VERSION     DADLCQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.239"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(226..429)
                     /locus_tag="JQG95_004079"
     CDS             complement(226..429)
                     /locus_tag="JQG95_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890433.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="JQG95_004080"
     CDS             complement(431..586)
                     /locus_tag="JQG95_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY5890434.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="JQG95_004081"
     CDS             complement(789..1196)
                     /locus_tag="JQG95_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890435.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="JQG95_004082"
     CDS             1273..1500
                     /locus_tag="JQG95_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5890436.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="JQG95_004083"
     CDS             1484..1906
                     /locus_tag="JQG95_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890437.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2628
                     /locus_tag="JQG95_004084"
     CDS             1984..2628
                     /locus_tag="JQG95_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890438.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEEEDLNTDLPLNPPRQKR
                     ASKKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGDTGTGKIPSAGFYT"
     gene            2774..3411
                     /locus_tag="JQG95_004085"
                     /pseudo
     CDS             2774..3411
                     /locus_tag="JQG95_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          882 a          750 c          918 g          861 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagattttta tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gttggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccttggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagaggaaga tctaaatact gatctccccc taaatccccc tcgccaaaaa
     2461 cgagcgtcta aaaaattcga gccggaggct attgagctgc ccgattggtt gccggaaaca
     2521 ctctggcatg agtgggtccg gttcagacag gcattgcgaa aaccgattcg aacggagcag
     2581 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
     2641 acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc ggcctgagac
     2701 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag
     2761 ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca tccgccgact
     2821 gaccccgcaa catgtaaccg ccccattcag aacggtagcg gagtggcgcg agtggcaact
     2881 tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt tgcgggtgga
     2941 aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt ttgcgaatta
     3001 ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca tcgccgatga
     3061 actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cctgataccg ggtagaatca
     3121 cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga ttgtggttac
     3181 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gatgggcagt caggcgaaaa
     3241 atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg gcattcagcg
     3301 cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga cagcgtcgat
     3361 gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat t
//
LOCUS       DADLCQ010000237         3285 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000237 DADLCQ010000000
VERSION     DADLCQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.240"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQG95_004086"
     CDS             complement(44..991)
                     /gene="icsP"
                     /locus_tag="JQG95_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY5890439.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2372..2524)
                     /locus_tag="JQG95_004087"
                     /pseudo
     CDS             complement(2372..2524)
                     /locus_tag="JQG95_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2580..3273
                     /gene="ospZ"
                     /locus_tag="JQG95_004088"
                     /pseudo
     CDS             2580..3273
                     /gene="ospZ"
                     /locus_tag="JQG95_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT         1036 a          607 c          576 g         1066 t
ORIGIN      
        1 gcaattttta gccatgaaaa cgtaatcaac tcggtgaacc aattcaaaaa atatacttta
       61 tacctgcgga agtgagaaaa ccaatatatt caatacttgc actattttta atggtgccag
      121 atatattggt actttgctca taaagagatg tatcaccttt tttattagaa atgtaatttc
      181 tggagccttc tatataaaat tttgcactgg gggtgacata gtatccaata ttcgcagcaa
      241 cagagcagta attttggttt tttatttcat ctttaaaaat tgtctcagtc tgatagtgtt
      301 tatctgtatc agatgaaaga acccaaccac tatatttcag ttctgctcca aactcaaaat
      361 tctcatggcg gtaattagca gttaatccaa tataaggtat tttgaagagc tgtttgtaac
      421 ctattgttct ttcaccacta ggatgtgccc cttttttatt cctgctcccc ccgttttcac
      481 tataaatata actccctccc attgcattaa aactgtaacg actctcttgg taacctgcta
      541 ttagtccaag tcgataatcc aaattattta ataaccaacc tttaagattc agctcgaatt
      601 cgttagcatc acggagatgc gtattgggat gccagctctg gtcggtccat acctgagggg
      661 tattggaatt gttccagtct ttatcaacca tgcttgcttt ctggttacct aaagtagtcc
      721 aaccggaaac tccgaaagac acttttggaa ttagcttcca atcgatgcca cctctaacaa
      781 tagtcgcatt actgtatttc cagtccagtt gactaatttt tctcccccct tccttgggat
      841 gataaacgcg ttcttttgtt ttgccactca gagatcctag actaatatcc gtactgatgt
      901 tgtccggtat gaaaagtgga tagttagtgg tagcatgtgt agtaaagatc gcaggtacac
      961 aaagtgcaag tacaaagaat tttaatttca tcgagaactc tacttttttg gttgaaatgt
     1021 ccatgatctt cttactttat aagataaacg tcaatgccaa gcattatttt aattaaatat
     1081 ttgtttatgt taccaagaac atatctttta ttataatatt tattgaactt ttgtaaaaat
     1141 aaaaaaatac ctcattgtgc gaataaagta acgggggcca tagatttctc tacctacctt
     1201 ttggtgttca tacctgaggc tgatagtttc aggattccgc gacaattcta ctcaccttct
     1261 tgttctctcg ccaatatgaa atcaatcaga aaagcgttcc ataagaacaa cgataagaca
     1321 ataaattgtc atcacggctg caatagccaa cgtgcctttc agtaccaaca tgacaacctc
     1381 ctgtatttga cgtaacgtgt gctgataaat atgtatttgt atctcaactg actcaataca
     1441 acacttaagt atagtttttc agattttgtt tttcccgtga aatattatgg aaatggcaga
     1501 agcaatacat tgaattccga ttcgagtgaa tacggctagc gggcacctca ctttagcact
     1561 gaagccatca acttttattt gacttatttc aatggaaagc tttctcacta tcagtccagg
     1621 taattcccat ccttttaaag tcgagagtca gactgcgact aatacagtta aagtgaccaa
     1681 actcagttat cctctacaca ttttatctca tgtttacttg gacaattcat tggtgctttt
     1741 ataaaagaat aaatattcta ctaatttaat gcatcaccta aaatagtatt ttttataata
     1801 gaactgtatc tatagtatat tattaaagct taattattta ttatgcttta atgtggttgt
     1861 ccgattaagg actaatcctt gaaaacgccg tatagggcga gagttcactc tattgcgata
     1921 acgtaaccgt acaatattgt gcgtttccca aattgaccac tactgaacta ttctgggatt
     1981 taaatgcaat aaaatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     2041 ttccagtgcc agatcttact tataataaag aggcttggca gtttggataa gctattgtcc
     2101 ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt tcatactgaa
     2161 atccccggaa gtattcaatc aaacaattac accataaaag gaagagggag ccccaattaa
     2221 gttatcaagc atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca
     2281 tataaaacgt ctttattgat cctacccaca taatatggac gcagtcgtaa gtgaggctct
     2341 gtgagcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2401 ccggactgag gccgccacac caacggtgcc gtcgccagcg attgtaattg cactcgatat
     2461 aattaaacac tgttgcccgc actatttccc gatagataaa gtgttctccg tggagacatt
     2521 ccacttagga acaattatat tgttcatggt aatgtttata tttgagtata gagattaaaa
     2581 tgattagtcc catcaagaat attaagaacg tatttccaat aaatactgca aatactgaat
     2641 atatagtgag aaatatttat cctagagttg aacatggtta ctttaatgaa tcaccaaaca
     2701 tatatgacaa gaagtatatc tctggtataa ccagaagtat ggcacaatta aaaatagagg
     2761 agtttattaa tgagaaatcc agacgactta attatatgaa gacaatgtat tctccatgtc
     2821 cggaagattt tcagcctatt tccagagccg aagccagtac accggaagga agctggttga
     2881 cagttatatc tggaaaacgt cctatggggc aattttctgt ggatagtttg taccatcctg
     2941 acttacatgc attatgtgag ctcccagaga ttagttgtaa gatcttctct aaagaaaata
     3001 gtgatttttt gtatataatt gttgtattca gaaatgattc tccccaagga gaactacggg
     3061 caaatcggtt tatagaacta tatgatataa aaagagagat tatgcaagtg ttaagagatg
     3121 agtcgccaga gattaaagtc tattaaatct gagattatta ttgctcgtga aatgggagaa
     3181 ctttttagtt atgcatcaga ggaaatagat agctacataa aacaaatgaa tgacaggctt
     3241 tctcaaatca aggctcgaat gcctgttact taatgtatat taatt
//
LOCUS       DADLCQ010000238         3258 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000238 DADLCQ010000000
VERSION     DADLCQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.241"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..441
                     /locus_tag="JQG95_004089"
                     /pseudo
     CDS             1..441
                     /locus_tag="JQG95_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1025..1741
                     /locus_tag="JQG95_004090"
     CDS             1025..1741
                     /locus_tag="JQG95_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890440.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2293..2697
                     /locus_tag="JQG95_004091"
     CDS             2293..2697
                     /locus_tag="JQG95_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890441.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2760..3258
                     /locus_tag="JQG95_004092"
                     /pseudo
     CDS             2760..3258
                     /locus_tag="JQG95_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          910 a          673 c          750 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagacccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccg gtttaatctg
      301 gtgataaagg cgttttacat gcgcctactt gcagccggaa acgccaaaaa agtggcactg
      361 gttgcctgta tgcgtaagtt gctgaccatt atgaacgcaa tgctcagaaa aaacgaagaa
      421 tggaatgaat cgtatttatg aaaactggat tgtgggccat aaatattcca aaaagatagc
      481 ccgctccgtt tattaccgga acgggcgaga gcatgacatt gtctgttatt ataatgagac
      541 tgctgattct acttattttt aatttcaaga aaagcgtttt ctacaaagaa ttatgagatt
      601 taatgttttg tttagacaat attatcattc tgttcaataa tgaaagtctt catcaacaac
      661 aagtaaaaac aacgtccctc tcatcgaacc ggacttgcag atttcccgca tccggctctc
      721 ttgcaacaca tcagaccttc acgttcgggc ggttatgggg aaggtccagc aagctgtatg
      781 agccctaact gtttgaatac cagtagatta ccagaaactt ctatcgattg aagacattac
      841 caactagcgt tactaaatgt tgaccaagtt ttgttcactt gtctggtttt gatagtagca
      901 ttctgcagag tattttgata tgataaatat gcattaacaa tgatattatt gttaatttcc
      961 tagcatgaaa attaaaagat caacttttat ctcgaatatt ttttacatta tcagttggtt
     1021 tcttatgaat gacaattcat tgcttagaaa ttcttcatta ttcatcgcct atatgggatg
     1081 tgtagggtgg gtcagtgctt attcttatgg ctggggaacc tctttttact atggatttcc
     1141 atggtgggtt gttggtgcag gtttggatga tgttgccaga agtttactct atgctatcat
     1201 agtaatgggt atacttttta ccggttgggg gattggtatt ttattttttc ttcttataaa
     1261 gaaaagaagt aaaatacaag acttgagttt ttttagacta ttttttgcta ttaccctact
     1321 gtttttccct gtaatattcg aacttttaat tctcaaacaa tatttcatac ttccattatc
     1381 actttcattc attattagtt cattggttat ttcaataata attcgtatat atggacggat
     1441 cttttctgta tcatgttttt cagatatccc ttttgttaga gaacaccgca ttaagttaat
     1501 tatggctggt tttttggttt atttttggtt tttttccttt cttgttggct ggtataaacc
     1561 tcaattaaaa aaagaatatc agatgctttg ttataataat agttggtatt atgttcttgc
     1621 tcgttatgat tcacggctcg ttttatctag ttcatttaaa gatgacagta acagattctt
     1681 aatatttaat acagaacagt cgggatttta tgaaatcaat gatgtttatg tcagaaagta
     1741 ataatcctct cccacaaagt gaattaaatg aatatataat gcgttcgaag tatatgtatt
     1801 gagctgtcat actgagaaac aaaccttagg gcagcacaat taaagccaga aacaactatg
     1861 caagaaagtt actaaataag tgctcggaac agtaaaacac tcagggggga ctcagtccgg
     1921 attatgtaat cagataaatt gccaaacatc acaaatcacc agccggactg agtgatgccg
     1981 agcattgcac caataccccg tgatgaacga tgcctgatgc agaaagctcc ctataaaaca
     2041 aagaacaaaa actacgaccg gcgattcacc gtcatactga tgctgcatcg tggtgacact
     2101 gtctgtcact ttcatgttga ctgaacaact ctgctgcgaa gaaggtgtac cgccggcggg
     2161 atttgattgt ccggcgtcta gcttccactt ccctgatgga actgaaagtt gttatggccg
     2221 gtcagcttgc cctgtccgca ggacatgagg tgctgtgacg gacagcacca gtaacggaaa
     2281 gtgacaatga aaatgaatac tgcgctgata gttgcactaa tgtgtatgtg gtatgcggta
     2341 cccgctgctg caaaagagac tttactggct atgccacgta attcaacaga acattgctat
     2401 gcggagatta atgtgcacgg accatatggg gtgtatttcc gagtggtccc gcatcccccc
     2461 gggggaaaga gctgggtcga atgtaacagt gactactatt actctgataa accaccggga
     2521 gtacagatac tcggaacccg tgcagggtgt cgggtatacg gaatctgcgg gaccacctcg
     2581 acactgcatg tggctggaag aggggttgtg tgtattaaaa atatatgcag tccacgtggg
     2641 atgataattc acaggatacg gaaacgtcct gtagtggctg taagcgatga gatgtgatct
     2701 tacccagcaa tagtagacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
     2761 tgacaaaaac agtatcaacc agcaaaaaaa cacgtaaaca gcattcgcct gaattttgca
     2821 gtgaagccct gaagcttgct gaacgcatcg gtgttgctgc cgcagcccgt gaactcagcc
     2881 tgtatgaatc tcagctctat gcgtggcgca gcaaactgca gcaacagatg acctcgtctg
     2941 agcgtgaaag tgaactgccc gcctgaagcg gcaactggcg gagcaggcag aggaactggc
     3001 tatcctccaa aaggccgcga catactttgc gcagcggctg gtatacgtgg tgtcagcgtc
     3061 ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt gtcctcgcgg
     3121 cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat gaactgcgtg
     3181 ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt caggggctga
     3241 gggcaaaggc ctcccgga
//
LOCUS       DADLCQ010000239         3178 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000239 DADLCQ010000000
VERSION     DADLCQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.242"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            417..1526
                     /gene="fepE"
                     /locus_tag="JQG95_004093"
     CDS             417..1526
                     /gene="fepE"
                     /locus_tag="JQG95_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY5890442.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1771..1905)
                     /locus_tag="JQG95_004094"
     CDS             complement(1771..1905)
                     /locus_tag="JQG95_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY5890443.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1930..2165)
                     /locus_tag="JQG95_004095"
                     /pseudo
     CDS             complement(1930..2165)
                     /locus_tag="JQG95_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2182..2364)
                     /locus_tag="JQG95_004096"
     CDS             complement(2182..2364)
                     /locus_tag="JQG95_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890444.1"
                     /translation="MRCEMLHRYVRRKCSFRRSSASSLTDSLRSVVRLRRAVSAHAKT
                     DIRLSTESGDNTRKNM"
     gene            complement(2551..2745)
                     /locus_tag="JQG95_004097"
                     /pseudo
     CDS             complement(2551..2745)
                     /locus_tag="JQG95_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          658 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca tcatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgccctggg ggcgctgcta ggggtgtctg ttcagatatg tagcaatgag tcagggattc
      121 acgaactgaa aatgtccgaa aatgtgtctc atgggcactg tatgcgccca aaccgatcgt
      181 cattcacttt catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg
      241 tttaccccgg ggaaaacatc tctttttgcg gtgtttgcgg cagaatcgcg ttcagcgcgt
      301 tttagcggtg cgcgcaataa aatgttatgt taaatgtctg ctgaatggtt tttaggtttt
      361 ttcctaacat atgatagaat aaaaaaacca tatgttaata tattgtgttt ttttcaatga
      421 aaatctcaaa ggtctctgac gaacattttt ctaattacaa catgttgccg aatggtaatg
      481 aagaaattga cctttttggc ttgattagcg ttttatgggc agcaaaaata aaaattgttg
      541 gggtgattct attttttgct tgtattggct ttcttgtttc ttttgttttg ccacaaaaat
      601 ggactagttc tgctgtagtt actcctgctg agtctgttca atggagtgaa ttaaataagg
      661 agctttccaa gcttcatgtt ctgggagtgg gttttgatat caatcgtgat agcgcatttg
      721 ctctgtttat taagaaattt cagtccgtta attcgttgaa tgagtatatg agatcatctc
      781 cttatgttat ggagattatt aaaaagaata acattagcgc tctcgaactg catcgtgcaa
      841 ttgttgagtt gagtaaaaat atgaagtcga ttgatgataa tgccagtaag aaaaacgaca
      901 agtcatcatt gtatgtatca tggactctga gttttactgc tccaacaagt aaagaagctc
      961 acgatgtgtt gtctgggtat attaattatg tttcttccct tgttgtaagg gatttgatgg
     1021 aagatataag aaataaacta gaagttaaaa ctaatgttga aaaagaaatt cttgcactgg
     1081 atgagataaa aattagaaac cagctgaatg cagatattcg acgcctcaat tattcactgg
     1141 aggttgctaa tgcggctgga ataaaaaaac ctgtatacag caatggtcag attatgaagg
     1201 atgacccaga ttttcctgtg gctctcggtt ctgatggtat agcaactaaa ttgaacatca
     1261 aaaaatcaat caaggatgtt tcggaattga gtggggagtt gcgaaatcgt caatatgttg
     1321 tgaatcaatt ggttgtggcg aaagtggggg atgttgattt tatgccattt cagtatcaat
     1381 tgagcccaac gttaccggtc agaaaggatg ggccaagtaa tgccataatt gtaattcttt
     1441 cttctgtcat tggagggatg cttgcatgtg gctatatact tttacgtcat gccatggagg
     1501 ccagaaagat gagtgtgatt gagtaaaagt tgtgctgatg catcatttta taagcaactt
     1561 ttataaaaag ttcaaaggat cttcttgaga tcattccctt acagccgtag tcttctactc
     1621 tagaaaacga aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac
     1681 ttctgaaccg tggtaacagt cttgtgcgag acatgtcacc aaatctgtcc ttctagtgta
     1741 gcctcagtaa ggctgccact tcaagaactc tcaatacatc tctcgcacat cctgtttacc
     1801 agtggccgct gccaatggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1921 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacgaacgc ggccataaca
     1981 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag
     2041 ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2101 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccaccg gcgcggcctt
     2161 ttttcggggc ctctcttttg ctcacatgtt ttttctggtg ttatcccctg attctgtgga
     2221 taaccgtata tccgtctttg cgtgagcaga caccgctcgc cgcagccgaa cgaccgagcg
     2281 cagcgagtca gtgagcgagg aagcggaaga gcgccggaag ctgcattttc tccttacgta
     2341 tctgtgcagc atttcacacc tcatatgtcg ctgttctctc accatctgct ctgccgcctc
     2401 ataattaagc cagtatacac tccgttatcg ctacgtgact gggtcatggc tgcgccccga
     2461 cacccgctaa aaccccctga cgcgccctga cgggcttgtc tgctgacgaa aacgcccacg
     2521 ggaaagccgt gaccgcctcc gtcagcttcg ttatgttcct gtttcacttt cggcctcaaa
     2581 gcgaatctgg atgctgttct ggagttcttc tgcgagttcg tgcagtcgtt tgcacatggc
     2641 cgcctgttcg tcggcatcca gagcatccag tttttcaagc agtgtcaggc tctggctttt
     2701 gatacatcgc gccatattca gcgcagtttg ttgttatttg ttcaccttcc gttgttctcc
     2761 gtcagttccg ggtgtcgggg gggggaccct gagcaggtgg taattgtaag acgttgcggg
     2821 cgatggtatt acaattgcac atcctgtcct gtttctttag gacgtatcat ggcacagcat
     2881 atcaactgat aaataattcc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
     2941 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
     3001 tgcctgaaat gcccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata
     3061 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
     3121 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcat
//
LOCUS       DADLCQ010000240         3169 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000240 DADLCQ010000000
VERSION     DADLCQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.243"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            93..3017
                     /locus_tag="JQG95_004098"
     rRNA            93..3017
                     /locus_tag="JQG95_004098"
                     /product="23S ribosomal RNA"
     gene            3101..>3169
                     /gene="rrf"
                     /locus_tag="JQG95_004099"
     rRNA            3101..>3169
                     /gene="rrf"
                     /locus_tag="JQG95_004099"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          846 a          695 c          979 g          649 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttattacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgcc
//
LOCUS       DADLCQ010000241         3101 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000241 DADLCQ010000000
VERSION     DADLCQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.244"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..218
                     /locus_tag="JQG95_004100"
     CDS             <1..218
                     /locus_tag="JQG95_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890445.1"
                     /translation="RFRAVATVRCVKTPGLQRPFRSRRLCRAVACAPGIPAKGRRNVR
                     RNAVSQTALHVVAAGPCSLPAGCHTPV"
     gene            706..936
                     /locus_tag="JQG95_004101"
     CDS             706..936
                     /locus_tag="JQG95_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890446.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            988..2349
                     /locus_tag="JQG95_004102"
     CDS             988..2349
                     /locus_tag="JQG95_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAY5890447.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2396..2959
                     /locus_tag="JQG95_004103"
     CDS             2396..2959
                     /locus_tag="JQG95_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5890448.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
BASE COUNT          715 a          863 c          921 g          602 t
ORIGIN      
        1 cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac cccgggcttg cagcgaccgt
       61 tccggtcgcg ccgcctctgt cgcgccgtgg cctgcgctcc cggcatcccg gcaaaaggcc
      121 gccggaatgt aaggcgcaac gccgtttcgc aaaccgcgct tcacgtcgtt gctgcgggtc
      181 cctgctcatt gcctgccggg tgccacacac ccgtatgagt ccctgtcgcc gggggcatag
      241 acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca aaatttttta
      301 cccaaaaacc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag
      361 tgatgcccct tgcggcgaag gttctcaaac gggtgaagtt cataccacag ggaatacagc
      421 agcaggttcc cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt
      481 aaaaatcagg atttatttca ggctccggca atacgcattt atttgttctg tcagtcatcc
      541 ggctggcagg taatgagaac gcaggaacag aaggaggaaa acaccggaga acgtcagacg
      601 cagatatgaa aaaagcaggc cggggaggac ctgcttaatc cacaccgcaa aaactgttcg
      661 agcagtttct gcggttccat tcaacattca taaggagttt catttatgac ggtaagtatt
      721 gtttcacctt ccgcagcggc tgtaaagccc cgccgtcatc cccgtttcag acgcgaagac
      781 atccccgcgc cggaaattga ccccgtgctg aaagccttcg ggcgacacat tgcccggagt
      841 tttcaccggg ggcgtggtgt acacatcccc gccatgaaaa atacggcttt cgggcaggtg
      901 ctccgcacac tcgaactgaa acgcgctttt aactgatgag gtgagccgcc ccgcgagggg
      961 cggcaacaga cggaaggaga acattttatg acattagcag caatcaccat gaccgccccg
     1021 gaagccgcca gccctgtgca gatgtaccgc gcgacctact caccggatga caacaaactg
     1081 cgcctgtatg ccgcgtcacg tcttgacccg gagacgtata aaaaagtgca tgatgccggt
     1141 tttcgctggg cacccagaca ggcgctgttt gtcgcgccag cctggacacc gggccgggaa
     1201 gacgtgctcc tctcacttgc cggagagatt gaggatgaag acagcacgct cgctgaacgt
     1261 caggaagcac gggcggagcg gtttaccgga tacagcggaa agcgggccag tgaatccgca
     1321 caggcacttg atgaagtgga aagactggcc gcgatgatcc cgcccggtca gcccattctt
     1381 gtggggcatc acagcgaacg ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg
     1441 aaacgtgccg tgatgctctt tgaacgtgcg gaatactggg aagaacgggc gcggtcggca
     1501 ctgcttcacg cgaagtataa agaacgcccg gacgttcgct ggcgtcgtat caaaaaaatc
     1561 gaagctgatt tgcgcaaggc tgaaaagacc atcgcacagt cgcagaaata tctgacgatg
     1621 tggcgggctg aatcgctgga tctgaatatg gcaaaactca tcagcagtca tgaccatatc
     1681 agcgcctgtt tcccgctgga tacgtatccg cgcccggcag aaaaaagcca gtatgaaggg
     1741 agccggtcgt tatggtcggc cctggatgat gacatcatca ccacggagca ggcccgcgaa
     1801 attgcgatcc gctgtcatga acggcagatt cagcatcagc aacgctgggt taaccactat
     1861 cagaaccgcc tgatctatga gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg
     1921 acacaggatt ttgagccggg cggacaggtt ttcagccggg gcgagtggct gactatcatc
     1981 cgcgtgaaca aaagcaacgg ggcggtgagt tcagtcacaa cgccaaatta cagttttctc
     2041 gggtacagcg gcacgatgaa agtgacgccc gatcgcatca cggactacaa agcaccatcg
     2101 gcagaagagg ctgccgtcgc cagtcaggcc gcgaagcgtc cgccggtagt caactatccg
     2161 ggggaaggtt tccgggaaat gacaaaggcg cagtgggccg ccctgccccg ggactgtaag
     2221 gccgtgcgca gtgtggaaga agcagaagac cacggggcat accgctaccg ccgcacaatg
     2281 gacaataatt tccgtctggt gaatgtgtat atcaccgaca tgaaaatcac ggaaatccca
     2341 cagaaataag gtatatcccc ccgggaaatc cccgggggat gacggagata atgacatgca
     2401 cagtcagtta agagaacgta tccggctgat gcgcgcaagg ctggataacg ccgcgccggt
     2461 tgctgaaatc cgggctgaat ctcagctttt tgtgagtcct gccccgatct gtgatcgcct
     2521 ggtgacgctg gcggaaatca gcaaccgtga tcacattctg gaaccctctg ccggcaccgg
     2581 ggccatactg cgggcgattc gggataccgc gccggaggcc atgtgtgatg cggtggagat
     2641 taacagcggg ctggtccggt atctgcggga aaattttaac ggtgtcaggg tacagtgtgg
     2701 cgacttcatg gaatggcagc cggtgcagta ttacagccgg atcattatga atccgccgtt
     2761 cagtcacggg caggatatcc ggcatatcct gcgggctttt tccctgttgc gtccgggggg
     2821 cgttctggtt gccgtctgtc tcaacgggcc acgccagcag gagaagctgt taccgttttc
     2881 tgacgtccgc gaggagctgc cgcgcggcac gtttgcttat accgatgtcc cgacgatgat
     2941 tattcgtttg cgtgcctgat gacacagggc tggcgggagg ataaatgccc gtctgtccgc
     3001 gccgggaaaa gaccgcgcgc cggtggcgct gaaaggggct gcgctcacgc gccgcccctt
     3061 tcattacgcg gacgttctga tgggggcttc tgtcttttca g
//
LOCUS       DADLCQ010000242         3098 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000242 DADLCQ010000000
VERSION     DADLCQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.245"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(105..1814)
                     /locus_tag="JQG95_004104"
     CDS             complement(105..1814)
                     /locus_tag="JQG95_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY5890449.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2246..2965)
                     /locus_tag="JQG95_004105"
     CDS             complement(2246..2965)
                     /locus_tag="JQG95_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY5890450.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          952 a          587 c          519 g         1040 t
ORIGIN      
        1 tgccaattga ggtggttgcc tgaaagttgg cagttacccg gtcactacac cttgttggag
       61 tgaacatatc aaaaacaggt aattacacaa tttaaattac agtctcataa aaaatgatta
      121 aatctattta ttatttcaat aaacgttttt ttcatcttct ttgaaaattg ttctgctata
      181 gtatccggtt ccagattgtt ctcttttatt attctacaat actctaatat tgtttctttg
      241 tctctattta ctacagatac aaatacacca ggaataccat ctttattagt tgcggacagc
      301 atttcgacca atcgtcttgt ggtaaaacta tagtttttta gaatattaca aaaggcacgt
      361 attgtttcgg tatggccatt ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt
      421 tttatccata ataattcatc tatatattcc tggtgagggg ggagtatttt tagtatatct
      481 ccatacgcca taatggcctt tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt
      541 actttcaata attctttaat ttgttcacta ctaagcattt tcttattaag cagacttccg
      601 aacatacgaa tactatctgc atgtccattc tgcaatgcaa aaaataaccc aggtaaatca
      661 tggctgtctt tcgcagctaa tagatataat ctcttatcgg aagttaacgg ggttttaaag
      721 actatttccc cataggcttt tattgtgtct acatgtccgt tcataaaaga catgcataaa
      781 cccggcacat tgtttgcccc taccgcagca agcaatgtaa gtattgtttc ttgactaaga
      841 ttgcacatat ttaatatctt accatatact tgaataatgt ctgaatatcc gttttgaagt
      901 gctatataaa atgcaggagt accatcagag cttctcgtgg ccagaaggtt caccagttta
      961 gattgagaaa tttcaccatt tttgtatttt tccatcagta taaataatac ttctgtcaga
     1021 ttctttgtta tcgcatgata tataactctg gaattgatca attcatctgg taaaagtttc
     1081 actgtgtttg ttagagggga attatgccta tcttcaaata ttacctgccc gggttgctcc
     1141 agaccataaa ttttccaata aaaatcatca aaaaaatcct tagcggataa ttgactcagt
     1201 tcgaaatggc tatcacaagt aaatacagta cgtttatgag taacagttcg attaggatcg
     1261 aaaaactgaa caacccattt ttcactttct tgtacgcgct ttattttcaa ccctaacgcc
     1321 atttggtggt ttaatgtgca tgctgataat gttctgacaa agtctccatt ttttttcatg
     1381 ttataaaaaa aattagtaat aactgttccc cactttttat tttccacaaa ataaatataa
     1441 gctggtgatt ttgaaaaatc ttccgttttt tccaaacata cattttttgc aatagcatca
     1501 ggggtgctaa gaagctgata gtttaccttc ccatttgttt ccataaaatt catttcccaa
     1561 taggaagcta ggtgacggca aactatttgt cttccatata tatcttttgc cactccattt
     1621 atatttacct ccacatttct cttttctgaa aaataaggtg ctgttttatt cttaaactga
     1681 attagttcac tggagtttga gaccctcata atagaacttg ttattgttct attatctctg
     1741 ttaacagaca tatctgttga taacgaattt tttgttgaaa atatactaga tgaaaatgtt
     1801 ttatttaacg gcatagaaga caaccataga gatgttataa tactattaga ttgcagttac
     1861 taatagtaaa agcctcctta caaggaggta gcttattcag aatgctccta taagatagcg
     1921 agctcgtcaa cgatacagat gatctgatta tattgtgcaa aaggagaaca ggaaaatagg
     1981 cactggaaaa gatgaaggcc tgatgggagc attaactaat gcaaaatccg acaggttaat
     2041 atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac gctgaaatgg tggacaactg
     2101 ttcggatttc agatgagtag ctcagcagtt tatgagccga atgctctgtc acgggcagga
     2161 atcaggctgc atacgcagtg ccaaatctaa tcgtagttat tctaaccagt tttcaagcta
     2221 caaggtggtg tagctggcat tttctctact ctatcatcaa acgataaaat ggttcctctc
     2281 gtaacatttg ctcttgcctt tcacttccat ctcgatcgct tcgtagttca ttacgatagc
     2341 tgacatattt ccagtcttca ggtctaacat ctgacgccgg atactccccg ggagcgattt
     2401 tgcttcttaa tagattagac tcgagacaca ttataaattg tcgtatttta tgaagtaata
     2461 aagcactata ttgcgagcat tcctgatctg attttacata tagcgtaaac tgagctccta
     2521 tccccacacg agattgctga gaaactcgat tcatatcagt tatcttccat ttatctatag
     2581 gactgtcctc tgaaaataat aaaccagaca aaatttggaa tgccagagga acttgctccc
     2641 tagctatact aatatgaaac ttgtccccaa caaaatcccc cttactctga ggattttcac
     2701 gacatgcatg aataaacaca tctccactat ggttcacaat ctggaatcct tggtacatga
     2761 cagaaaaaga ttgacatata tcagagtaag ttggcacgtt cgatgctact ttgaaatagt
     2821 acgctgggta ttgccgtatt tggttgtaca atatattgaa gttatttttc aatctttcat
     2881 tcgcagaaag catctgcggt atttcagacc tcacaacatt caaagaatct aaatttagtt
     2941 ttagacaggg cttttttatg ggcatagaaa acgtcctcta taaaatagat atattatctt
     3001 atattgattg tttatcatct tttataaaac gatacattat atgtaatgta tgtaagcgta
     3061 agggtcctct ccgatcatgg tgggaaggct tagaacgc
//
LOCUS       DADLCQ010000243         3066 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000243 DADLCQ010000000
VERSION     DADLCQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.246"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            542..1708
                     /gene="nhaA"
                     /locus_tag="JQG95_004106"
     CDS             542..1708
                     /gene="nhaA"
                     /locus_tag="JQG95_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY5890451.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1774..2673
                     /gene="nhaR"
                     /locus_tag="JQG95_004107"
     CDS             1774..2673
                     /gene="nhaR"
                     /locus_tag="JQG95_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY5890452.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2711..>3066)
                     /locus_tag="JQG95_004108"
     CDS             complement(2711..>3066)
                     /locus_tag="JQG95_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890453.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          721 a          678 c          753 g          914 t
ORIGIN      
        1 atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg ggcttttctc
       61 tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg ctattatatc
      121 gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc gcatttttgt
      181 ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat cgcaattatt
      241 gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga ctgacaacaa
      301 attaattatt acttttccta attaatccct caggaatcct caccttaagc tatgattatc
      361 tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg
      421 cgcaaattct tcaatggctc gtaaaaaacg aattattcct acactataat ctgattttaa
      481 cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa
      541 agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat
      601 tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat ggtatcacga
      661 ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct
      721 gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa
      781 acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc cagttatcgc
      841 cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact atgccgatcc
      901 gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg cacttggtgt
      961 actggcgctg ttgggaagtc gtgttccgtt agtgctgaag atctttttga tggctctggc
     1021 tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc
     1081 gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg
     1141 tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga ctgcggtgtt
     1201 gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta ttcctttgaa
     1261 agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc
     1321 gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac
     1381 gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa
     1441 accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc
     1501 tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta tcggttttac
     1561 tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg
     1621 ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt
     1681 acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct
     1741 cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg
     1801 tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga ggcgctttat
     1861 ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct gcaaggcaaa
     1921 ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact ggtctatcgc
     1981 tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa ctatcgcaaa
     2041 gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg cctggtcagt
     2101 agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg cttcgaatcc
     2161 acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat catttctgac
     2221 tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc
     2281 gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg tctggaagaa
     2341 cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct taactggttt
     2401 aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc tttgatgaaa
     2461 gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt
     2521 tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat
     2581 gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat
     2641 tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa gttaaggtgg
     2701 gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt tgcccgcttg
     2761 agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat
     2821 ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat
     2881 tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta
     2941 atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg
     3001 gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact
     3061 gataat
//
LOCUS       DADLCQ010000244         3060 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000244 DADLCQ010000000
VERSION     DADLCQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.247"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="JQG95_004109"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="JQG95_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY5890454.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
     gene            complement(919..1014)
                     /locus_tag="JQG95_004110"
                     /pseudo
     CDS             complement(919..1014)
                     /locus_tag="JQG95_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="JQG95_004111"
     CDS             complement(1050..1355)
                     /gene="ccdB"
                     /locus_tag="JQG95_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY5890455.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="JQG95_004112"
     CDS             complement(1357..1575)
                     /gene="ccdA"
                     /locus_tag="JQG95_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY5890456.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2111..3060)
                     /locus_tag="JQG95_004113"
                     /pseudo
     CDS             complement(2111..3060)
                     /locus_tag="JQG95_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          763 a          744 c          736 g          817 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataataa ggataaacaa
      781 aattggctgt aaaaaaacga tactgtttca gttaaccgtg aatggcctgt ttgcaccgta
      841 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacggt aagcggctcg
      901 tcagaaccgt attgatattt actgagaggt cagatcaact ttccaaggca acagatcgcg
      961 tacccggttt gccggccagt cctggatatg ctcaatgacg taacgcagcc attttgtcaa
     1021 cgccacgatt tttgacccta gcctgacatt tatattcccc agaacatcag gttaatggcg
     1081 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacgaagatc
     1141 ggcacactgg ccatatcagt ggtcatcatg cgccagcttt catccccgac atgcaccacc
     1201 gggtaaagct cacgggagac tttatctgac agcaggcggg cactggccag ggggatcacc
     1261 atccgtcgtc cgggcgtgtc aataatgtca ctctgtacat ccacaaacag acgataacga
     1321 ctctctcttt tataggcgta aaccttaaac tgcatttcac cagtccctgt tctcatcggc
     1381 aaaagagccg ttcatttcaa taaaacgggc gatctcagcc atcccctgct gattttcagc
     1441 ctgccagcgt tccgcacgca gacggcgggc ctctttttgc atggccgtat ttaccaggcc
     1501 tgagatattc acgtttgcgg atttaagtaa ctgatagctg tcactgtcta tagtgacagt
     1561 aatacgctgc ttcatgataa gccctcaaaa acatacttat gatatgtata tgagtatata
     1621 ttaatggtac aaaccatcaa tatccataaa aagtacacca ttaattcgct ataagttttt
     1681 aatacaattt tttctgctgc gattttttta tactataatt agcatactta taacaccgta
     1741 agtaaattac atgaaagcag gcacagcgtg atccgatttt tctgcgtata acgattttcc
     1801 ggaagtgtaa tacggttgcg ccccgggccg ggcacccttt tctgttgttg tgtacgggcc
     1861 cacagagaat ggtaccggag gcgggctcag aagtactgag tcgggtgatc gcgtcaggcc
     1921 gcgaagtact gacacgggta ttggtgactg gtgatgaagt actgccccgg gtatgatttc
     1981 aggctgattc aggttctgat tctctgtcag tccgcgtggt tgctggcctg gctgatttcc
     2041 tgttgtcagg cgttcaacaa ccgactgcag gttatagccc ggttcacgaa cacctgacgg
     2101 caggtatcac tcacccggtg aaggtaaatc cctcaagagg cagcaactct gccagtcgct
     2161 cctccggcca ttccggcaga cgcgtcagga cgtccgtcag ccaggcatgt ggctccagac
     2221 cattacgttt tgctgtttcc agaaggctca ttatctgtgc cgcccggttt ccggccatca
     2281 gtgaaccggc gaacagccac gctttccgtc ccataacaac cgggcgaatg gcacgctcgc
     2341 agatattgtt gtccaggggc accgctccgt cacccaggaa gcagctcagt tcatcgcgcc
     2401 ggttcagaat atagttaatc gccttattta atggcccgtc gggcggacag cacggctcct
     2461 gctcttcaag ccaggcaaac aggtcattca ctatcggccg ggagtaccgt tatcgccact
     2521 gccgtatttt ttccacaggg cgctcacgga tgagtttctc aatgcggtaa agacccgcga
     2581 tttttctcag ctccacgcct gcacggggct ccctgctggc cttatacaga tttgcgaagc
     2641 cgcgccctgc gtgactccag caacaggctg atgtgatgcc ggggtgatta tccgccagag
     2701 atttgtacac gctgtatccg tcactcacca gcgtactgcc acaccagcac tgcagccagg
     2761 tctcagggta tctgagggcc cgtccggtct gcgagtcaaa acagacgacc ggcggaccgc
     2821 ttctttcacc gctgacgtaa gcacacagcc agccggagca ggatttaccg cctttccggg
     2881 tatcaaggag cctcagggac gtttcatctg catgaataac atcccgtgtc agaagctcat
     2941 ggcgtagcag tttcgccagc ggggccagtg cagctccggc aacaccgacc atatccgcca
     3001 ttgtactgac ggggagctcc acacccattc gggagaaaat gtgctgctgg cgatacaggg
//
LOCUS       DADLCQ010000245         3043 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000245 DADLCQ010000000
VERSION     DADLCQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.248"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..514)
                     /locus_tag="JQG95_004114"
     CDS             complement(<1..514)
                     /locus_tag="JQG95_004114"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="reversible synthesis of carbamate and ATP from
                     carbamoyl phosphate and ADP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY5890457.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIEL"
     gene            complement(511..1158)
                     /locus_tag="JQG95_004115"
                     /pseudo
     CDS             complement(511..1158)
                     /locus_tag="JQG95_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            1161..2393
                     /gene="allC"
                     /locus_tag="JQG95_004116"
     CDS             1161..2393
                     /gene="allC"
                     /locus_tag="JQG95_004116"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY5890458.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            2404..>3043
                     /gene="allE"
                     /locus_tag="JQG95_004117"
     CDS             2404..>3043
                     /gene="allE"
                     /locus_tag="JQG95_004117"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY5890459.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT          759 a          792 c          800 g          692 t
ORIGIN      
        1 acaactcgat ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc
       61 gcaaatattt accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt
      121 cttctggctg ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag
      181 gcgaaacctc aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca
      241 agctctgcgc cagcatatag ccaatcatcc cctggctttc cgccaccagt acatccagcg
      301 gatacggttc tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc
      361 cgttgccgtg aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag
      421 cactggcgat attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg
      481 cgttgccccc aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc
      541 aagccagaac cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc
      601 aatcgcaaca gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga
      661 ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag
      721 ttgaagattg tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata
      781 ataccaggcc gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg
      841 gcctttaccc agccagtacc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca
      901 gtgacgaaac tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc
      961 gaaaagtccg gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga
     1021 aggcggcgta atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag
     1081 gcgaatccct tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc
     1141 atcgaattgc gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct
     1201 ggctttcctc ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg
     1261 aatggctgga aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac
     1321 gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag
     1381 tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat
     1441 tcggcgcgct ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt
     1501 tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct
     1561 tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg
     1621 atgccaaagg aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgccgaacg
     1681 ccccactaac tccgcgtcag gctataaaag cctttgttga actgcatatt gaacagggct
     1741 gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc
     1801 gctatacggt gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc
     1861 gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa
     1921 agatgggcga tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa
     1981 atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc
     2041 tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca
     2101 ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg
     2161 tcgccaccct gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg
     2221 gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc
     2281 ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg
     2341 aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca
     2401 taaatgggat atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg
     2461 attgttaaac acggtaattt cgcactgtta accccggatg gtctgataaa aaatattatt
     2521 ccggactttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt
     2581 gttgattatc tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa
     2641 ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa
     2701 acatttgcct taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt
     2761 gttaacgccc aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta
     2821 gaaggccatg caccgtggct gttttctggc aatgccagcg aactggaacg cattcactat
     2881 gaaggtatgg atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg
     2941 aacatgcata tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt
     3001 caggaacatg gtgcctatat tctttccggt cagggggttt ata
//
LOCUS       DADLCQ010000246         3036 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000246 DADLCQ010000000
VERSION     DADLCQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.249"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..237
                     /locus_tag="JQG95_004118"
     CDS             <1..237
                     /locus_tag="JQG95_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY5890460.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="JQG95_004119"
     CDS             247..885
                     /locus_tag="JQG95_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY5890461.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="JQG95_004120"
     CDS             888..2051
                     /locus_tag="JQG95_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY5890462.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..>3036
                     /locus_tag="JQG95_004121"
     CDS             2135..>3036
                     /locus_tag="JQG95_004121"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY5890463.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRF"
BASE COUNT          689 a          715 c          868 g          764 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acattttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt gttaagccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcgatttt ctagccagta ttggtcagca
     2281 gttaggaacg gcagaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccacccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttga
//
LOCUS       DADLCQ010000247         2951 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000247 DADLCQ010000000
VERSION     DADLCQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.250"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="JQG95_004122"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="JQG95_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY5890464.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="JQG95_004123"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="JQG95_004123"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY5890465.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="JQG95_004124"
     CDS             2603..>2951
                     /locus_tag="JQG95_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY5890466.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          702 c          779 g          812 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DADLCQ010000248         2924 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000248 DADLCQ010000000
VERSION     DADLCQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.251"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            9..779
                     /gene="lsrF"
                     /locus_tag="JQG95_004125"
     CDS             9..779
                     /gene="lsrF"
                     /locus_tag="JQG95_004125"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY5890467.1"
                     /translation="MQSRLSWIFNPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLF
                     EHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCA
                     VAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAA
                     EMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMG
                     RNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="JQG95_004126"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="JQG95_004126"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY5890468.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1132..1890
                     /gene="tam"
                     /locus_tag="JQG95_004127"
     CDS             1132..1890
                     /gene="tam"
                     /locus_tag="JQG95_004127"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY5890469.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1894..2808)
                     /locus_tag="JQG95_004128"
     CDS             complement(1894..2808)
                     /locus_tag="JQG95_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890470.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEEPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          771 a          678 c          779 g          696 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactac cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aatgtctgac
     1141 tggaacccct ctttatatct gcacttttcc gctgaacgat cgcgtccggc ggtggagctg
     1201 cttgccagag tgccgctgga gaatgtcgaa tatgttgccg atcttggctg tggcccaggt
     1261 aacagcaccg ccctgctgca tcaacgttgg cctgcggcca ggataacagg catcgactcg
     1321 tctccggcga tgattgctga agctcgcagt gctttgccag actgccagtt cgtggaagca
     1381 gacatccgca actggcaacc ggaacaggca ctcgatctga tttttgctaa tgcctcactg
     1441 caatggctgc ccgaccacta cgaattgttt cctcacttgg tttctttact taatccgcag
     1501 ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt gctcatgcgc
     1561 gaagttgctt gggaacaaaa ctacccggat cgcgggcgtg agccgttggc tggcgttcat
     1621 gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg aaccacttac
     1681 tatcaccaga tgccgtcgca ccaggcgatt atcgattggg tgaccgccac tggattacgt
     1741 ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttacgcg ctaccatcag
     1801 atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc atttccgcgt
     1861 ctgtttattg ttgcccgccg tacggagtaa ttatcacgtc agctggtaat gacgatcggg
     1921 aagaattttt gctggaattt ctgcttcatc gttcatctgt aacaggtcaa tttcaatagc
     1981 gttgcagatg gcatccagcg gtaaatcatt gttttcagta ccgaacggtt cttccagttc
     2041 ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga taaaaggcgt
     2101 catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca gataaacggt
     2161 acgatgcaaa atcagggtgt aggcaaaggg aattggcgtg taggcaatgc gctcacagcc
     2221 tgccagaacc gctgaaatat cattaagccg atcgttgagg ctaataaaca ggatatctga
     2281 aagctgtcca ttgcggcgct gaaccgccaa ccattctccc attattaaca agatacggtt
     2341 agctggagag ttcgaagcca gtacacgctg aagatctcca gtcttgagat aatgagccag
     2401 cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga aggcgatttg
     2461 cagcctggca aactccctta cacttgccga atccggcaat gtcgttttta cctctcgcag
     2521 taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa cgtaacgggc
     2581 gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga gaatgctgaa
     2641 cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag ggcagcatga atataacagc
     2701 aatagaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata ctgagccgtg
     2761 ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg ttatcttcag
     2821 gcgtggaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac gctacttaca
     2881 aattacctag aaccacggca gcctgacggc tgcaaattga aaac
//
LOCUS       DADLCQ010000249         2697 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000249 DADLCQ010000000
VERSION     DADLCQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.252"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JQG95_004129"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JQG95_004129"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY5890471.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JQG95_004130"
     CDS             complement(1725..2096)
                     /locus_tag="JQG95_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY5890472.1"
                     /translation="MTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JQG95_004131"
     CDS             complement(2119..2463)
                     /locus_tag="JQG95_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY5890473.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          666 a          649 c          674 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctggtccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLCQ010000250         2690 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000250 DADLCQ010000000
VERSION     DADLCQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.253"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            230..769
                     /locus_tag="JQG95_004132"
     CDS             230..769
                     /locus_tag="JQG95_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY5890474.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="JQG95_004133"
     CDS             complement(995..1318)
                     /locus_tag="JQG95_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY5890475.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="JQG95_004134"
     CDS             1604..1798
                     /locus_tag="JQG95_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY5890476.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            complement(2119..2386)
                     /locus_tag="JQG95_004135"
                     /pseudo
     CDS             complement(2119..2386)
                     /locus_tag="JQG95_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcagggg caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttgatagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DADLCQ010000251         2681 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000251 DADLCQ010000000
VERSION     DADLCQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.254"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..848)
                     /locus_tag="JQG95_004136"
     CDS             complement(<1..848)
                     /locus_tag="JQG95_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY5890477.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGDHSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKVPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDN"
     gene            complement(863..1600)
                     /locus_tag="JQG95_004137"
     CDS             complement(863..1600)
                     /locus_tag="JQG95_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY5890478.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVAGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            complement(1488..>2681)
                     /locus_tag="JQG95_004138"
     CDS             complement(1488..>2681)
                     /locus_tag="JQG95_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY5890479.1"
                     /translation="ESQLDELQIWDALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLE
                     TIGEGKFKGILPLDRWMINPVLTRRIKEMGPDLGKPEFYDVVTTATGIPAWRIHHSRL
                     IRFDGVTLPFQQKMTENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKL
                     RELIALGGPAFEALLKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGLDDILSQFAE
                     QISGAVGIPLVRLFGQSPKGFSTGDADLANYYDRVSSLQERRLRLPMRRILDIMHRSE
                     LGKPLPDDFTFEFNPLWQMSDVDRSTVAVNTTNAISTALGDGLMTRKAAMTDLRENSD
                     VTGIGASITDEDIENAEDEAPPGIGELGDKPPEPPGGDPISNEPTADSAGGRGYRKWA
                     LRWFK"
BASE COUNT          518 a          790 c          722 g          651 t
ORIGIN      
        1 ttattatcgt tatcgcccgt ggcatttcct tccagcttag ccagcagggc tttcagtaat
       61 gttttgaggt catcatcact gtcgccggtt ggacctccgc ccatctctgg tactttgtcc
      121 ggtagcggtt gctgcgggga caggttgatg ttgagattaa cgccctgcgg caaatccccc
      181 tcatctcctg taaccgatgc gggagccgac tccaccagtt cgttcatggt gtcagcgtca
      241 cccgttttga tggccgtgcg catgcgggtc caccagcttt tcttttgatt tgccattgtg
      301 tctctgtctc caattgcaca acgatttccg gctctgcctt tggggacaag agccacatgg
      361 tttccggtaa tatcgacctg ctcggcttta cctggctcgg tctgctcgta ctccgcgtca
      421 tagccgcacg acacttcacg caggccatct tcgataagct gaatggcgtt ttcgtctttg
      481 acgataaggt cagccagcat caaatcagag tggtcacccg tcccgcgccg cacattctga
      541 agatgcccga cagcaagctc tttccagttc tcgggattta ccagccgcac attcccgttt
      601 tcatcttcag gatgcaggat cgtgatgctc atcccttcga atgaggcgag cgtggccgga
      661 tggaatacct gctcaggaga gcgcgttacg actatctcac cgagcttgtc gggtttgagg
      721 tttggcagat cggcagcgcc gtagagctgt ttacccgttc gacctatcgg cacgtctttg
      781 cacagcagcg agccgtcagc cagctgatag cgggtttccc ccagccgggt attgaaaaaa
      841 tatttcatgg tttacctgcg attcaggcga gataagaatg agggttggga aaaacgattt
      901 ctttataaca gcgacaattc gggagctcgc cagcgtgacc ggtcatgcca tcaagcgttg
      961 gaggtttgcc ccattcgaca aacttacctt ccatctcccg atgagaatgc cggacgtcgc
     1021 catcttcggc tgtacgccag atataaccat tcgagccgat tgacagcgca cgcgcctgat
     1081 cgagcgcgcc ggttgcacgt ccaagctcgg tacgggcgat aaggttcgct cgtgagcgtg
     1141 acacgtcacc ggacgctgct atctctttcg cgaatggttc agcgcggcca ccagccacaa
     1201 cggcctcgat ggccttgttc tgaatgtcat acacccgatc ggcggcctca agaggcagtg
     1261 acttgatata cttaatttgc tcggcgacga tggatttcat cacctggcct accggggcgc
     1321 ggtcgaccat gttgcgcagc tctgcgctga tgttccggct gtgctgacgc cactgctttt
     1381 cattctggcg tgcaatgtcg gcggtaaagt tctcagcaac cttcgtcgcc cagggggtga
     1441 tgatttcgct gtagcgctcc agcgcatcca ttatttcggt aacgctatca tttgaaccat
     1501 cgtagcgccc atttacgata tccccgaccg cccgcgctat ctgccgtagg ctcgttcgat
     1561 atcggatctc cgcctggcgg ctctggcggt ttgtcgccaa gttcgccgat gcctggcggc
     1621 gcttcgtctt cggcattctc gatatcctcg tcggtaatgg atgccccgat gccggtgacg
     1681 tcagagtttt cgcgcaggtc ggtcatcgcc gccttacgcg tcatcaatcc gtcgcccagc
     1741 gcggtactaa tcgcgttggt ggtgtttacg gccaccgttg atcggtcaac gtctgacatt
     1801 tgccatagcg ggttaaactc aaacgtgaaa tcgtccggca gcggctttcc gagttccgag
     1861 cggtgcataa tgtccagtat ccggcgcatc ggcagccgta agcggcgctc ctgcaatgag
     1921 ctcacccggt cgtaatagtt ggcgaggtct gcatcaccag tagagaagcc tttcggggat
     1981 tgaccgaaca accgcaccag cgggatacca acggcaccgc taatctgctc agcaaactgc
     2041 gaaagaatgt catccagacc actaaagctg tactggtggg tttcgaactt atcccgcgag
     2101 tccatgagcg tcataccttc attgctctgg aactggcgga tcaggtcgat gttcttcagc
     2161 aacgcctcga acgccgggcc tccaagcgcg ataagctcgc gcaacttctc cacgctatag
     2221 gtacgcagat gtgctttgta gaccagctgc gccgcgccga cagtggcgct gtcgaacgca
     2281 gtaagccgat cccagatacg ctctacaacc gacattcccc attcgttttc ggtcatcttc
     2341 tgctggaatg gcagcgtcac cccgtcgaag cgaatcaggc ggctgtgatg gatgcgccag
     2401 gccgggatgc ccgttgcagt ggtcaccacg tcgtaaaact caggtttgcc gagatctggc
     2461 cccatctctt taatgcggcg ggtcaggacc gggttaatca tccagcggtc gagcgggaga
     2521 atgcccttaa acttgccttc tccaatggtt tcgagccgca gcggggtcat tggtgcctgc
     2581 ccctcgatca tgatgaagcc gaccgcgccg ccgtagaggc gagaccattt cagcacgtcg
     2641 ttcagcgcat cccagatttg caactcatcc agttgtgatt c
//
LOCUS       DADLCQ010000252         2614 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000252 DADLCQ010000000
VERSION     DADLCQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.255"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            273..533
                     /locus_tag="JQG95_004139"
     CDS             273..533
                     /locus_tag="JQG95_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY5890480.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            757..831
                     /locus_tag="JQG95_004140"
     CDS             757..831
                     /locus_tag="JQG95_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY5890481.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            824..1681
                     /gene="repA"
                     /locus_tag="JQG95_004141"
     CDS             824..1681
                     /gene="repA"
                     /locus_tag="JQG95_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY5890482.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2381..2572
                     /locus_tag="JQG95_004142"
     CDS             2381..2572
                     /locus_tag="JQG95_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890483.1"
                     /translation="MADSGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          686 a          690 c          645 g          593 t
ORIGIN      
        1 gctgatcagc agtcaccgga acatcgtagc tgacgccttc gcgttgctca gttgtccaac
       61 cccggaaacg ggaaaaagca agttttcctc gctcccggcg tttccataac tgaaaaccat
      121 actatttcac agtttaaatc acattaaacg acagtaatcc ccgttgattt gtgcgccaac
      181 acagatcttc gccacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga
      241 aacgatccct gtttgagtat tgaggaggcg agatgtcgca gacagaaaat gcagtgactt
      301 cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc tatgactgac tctgagaaac
      361 aaatggccgc tgttgcaaga aaacgtctta cacacaaaga gataaaagtt tttgtcaaaa
      421 atcctctgaa agatctcatg gttgagtact gcgagagaga ggggataaca caggctcagt
      481 tcgttgagaa aatcatcaaa gatgaactgc agagactgga tatactaaag taaagacttt
      541 actttgtggc gtagcatgct agattactga tcgtttaagg aattttatgg ctggccacgc
      601 cataaggtgg cagggaactg gttctgatgt ggatgtacag gagccagaaa agcaaaaacc
      661 ccgataatct tctttaactt tggcgagtca gaaagattac cggggcccac ttaaaccgta
      721 tagccaacaa tcaagctatg cggggagtat agttatatgc ccggaaaagt tcaagacttc
      781 tttctgtgct cgctccttct gcgcattgta agtgcaggat ggtgtgactg atcttcaaca
      841 aacttattac cgccaggtaa agaacccgaa tccggtgttc acaccccgtg aaggtgcccg
      901 gacgcttccg ttctgcggga aactgatgga aaaggcggtg ggcttcacct cccgttttga
      961 tttcgccatt catgtggcac atgcccgttc gcttggtctg cgtcggcgca tgccaccggt
     1021 actacgtcgc cgggccattg atgcgctgct gcaggggctg tgttttcact atgacccact
     1081 ggccaaccgc gtccagtgtt ccatcaccac gctggccatt gagtgcggac tggcgacgga
     1141 gtctgctgcc ggaaaactct ccatcacccg ggccacccgg gccctgacgt tccttgcaga
     1201 gctgggactg attacctacc agacggaata tgacccgctt atcgggtgct acattccgac
     1261 cgatatcacg ttcacatctg cactgtttgc tgccctcgat gtatcagagg aggcagtggc
     1321 cgccgcgcgc cgcagtcgtg tggaatggga aaacagacag cgcaaaaagc aggggctgga
     1381 taccctgggt atggatgaac tgatggcgaa agcctggcgt tttgttcgtg agcgtttccg
     1441 cagttaccag acagagctta agtcccgtgg aatgaagcgt gcccgtgcgc gtcgtgatgc
     1501 ggacaggcag cgtcaggata ttgtcaccct ggtgaaacgg caactgacgc gtgaaatctc
     1561 ggaagggcgc ttcactgcca gtcgtgaggc ggtaaaacgt gaagtggagc gtcgtgtgaa
     1621 ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg ctggccacag cttccccctg
     1681 aaagtgacct cctcagaata atccggcacg cgccggaggc attcgcacgc ctgaagcccg
     1741 ccagtgcaca aaaaatcagc gctacataca aaaaacaacc tcatcatcca ccttctggtg
     1801 catccggtcc cccctgtttt tgatacaaaa cacgcctcac agacggggaa ttttgcttat
     1861 ccacattaaa ctgcaaggga cttcaccata aggttacaac cgttcacgtc ataaagcgcc
     1921 agacgtcaac cctacagggt gcaatgtatc ttttaaacac ctgtttatat ctcctttaaa
     1981 ctacttaatt acattcattt aaaaaaaaaa cctgttcact gcctgtcctg tggacagaca
     2041 gatatgcacc tcccaccgca agcggcgggc cccaaccgaa gccgatttag ttacaacatg
     2101 caaacataaa cccctaaaaa acccaagtcc aacacagaac tgaaaccaca acgcccccct
     2161 cataactgaa aagcgccgcc gcccccgccc aaagggcggg aacagagtcg ctattaatta
     2221 tgaatgttgt aactacatca tcatcgctgt cagtcttctg gctggacgta tcgagtacac
     2281 gctcgtaagc ggccctcacg gcccgctaac gcggagatac gccccgactt cgggtaaaac
     2341 cctcgtcggg accactccga ccgcgcacaa aagcattttc atcgccgata gcgggtatgg
     2401 cttggcaggg ctggggatgg gtaaggtgaa atctgtcaat cagtaccggc tgacgccggg
     2461 cttcggcggt tttactccgg tatcacatgt aaccacagcg tgccgccttc catgccgctg
     2521 gcgcggcatc agaataatac aagcagcatt taatgcattt gcaaaagtgt gagtaatatt
     2581 aagtggtcgt ttcagggata tcgttaagac ttga
//
LOCUS       DADLCQ010000253         2572 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000253 DADLCQ010000000
VERSION     DADLCQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.256"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(64..822)
                     /locus_tag="JQG95_004143"
     CDS             complement(64..822)
                     /locus_tag="JQG95_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY5890484.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JQG95_004144"
     CDS             complement(830..1933)
                     /locus_tag="JQG95_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY5890485.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2572)
                     /locus_tag="JQG95_004145"
     CDS             complement(1943..>2572)
                     /locus_tag="JQG95_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY5890486.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLATAIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          710 a          682 c          602 g          578 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggtggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aa
//
LOCUS       DADLCQ010000254         2482 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000254 DADLCQ010000000
VERSION     DADLCQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.257"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..566
                     /locus_tag="JQG95_004146"
     CDS             <1..566
                     /locus_tag="JQG95_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY5890487.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            524..1201
                     /gene="bglJ"
                     /locus_tag="JQG95_004147"
     CDS             524..1201
                     /gene="bglJ"
                     /locus_tag="JQG95_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY5890488.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLSRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="JQG95_004148"
     CDS             complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="JQG95_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY5890489.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2168..2404
                     /locus_tag="JQG95_004149"
     CDS             2168..2404
                     /locus_tag="JQG95_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY5890490.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          635 a          546 c          644 g          657 t
ORIGIN      
        1 tagtcattgc tgatttagcc ggtgaaagtg aagatccacg ttctgtttgt gaacattatt
       61 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      121 attcccaggc tgtagaactg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      181 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcacgcagag cgtatcagcg
      241 ccatgctgac ctctccggca atgactgaaa ctcatgattt tagctatcgc tccgtcattc
      301 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      361 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      421 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      481 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      541 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
      601 tttatccaga aagtttgcgg gcaaccctta taagctccat acctatacca gtcaggagtc
      661 atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
      721 gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta agtttccgcg
      781 tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg gttcattgtc
      841 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
      901 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
      961 cattaaccaa agccggacgt taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1021 tggctactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa cgatccgagc
     1081 gcataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1141 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1201 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatagcca
     1261 cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac cagcaggccg
     1321 tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cgttcgtgag cgttttctca
     1381 aaaaagaggg catggcgcag cgattcaaca gtagcctcgc cgagcagttg cttcatctca
     1441 gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1501 ccggtcgctt ctagtgcttg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1561 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     1621 gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac atctaatgct
     1681 ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat ataccattgt
     1741 gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt gcgatagata
     1801 tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     1861 gtcatggcgt taagcggagc gggttcatcc aggcggataa actccagcaa atgttcacga
     1921 tgtttggcga tcatcgcccg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     1981 gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat cgtgatagta
     2041 atctaaatga taatgattgc taatcctagc gataggttta ctcgatagca agggatttct
     2101 ctggcttgca aatgataaaa attatcatat gatattggtt ttcattatca atgaaagaga
     2161 tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc cgggattgct
     2221 gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg tcattctgat
     2281 gggattagta ttgacgccgg tgatgctgta tcacaaacag ttgcggcatt acattttgct
     2341 accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt tgaatcaggg
     2401 atgacaaaac agggaagaaa caagaaagga agtaaagata attggtgcga ggggggggac
     2461 ttgaaccccc acgtccgtaa gg
//
LOCUS       DADLCQ010000255         2432 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000255 DADLCQ010000000
VERSION     DADLCQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.258"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            65..1543
                     /locus_tag="JQG95_004150"
                     /pseudo
     CDS             65..1543
                     /locus_tag="JQG95_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            1540..>2432
                     /locus_tag="JQG95_004151"
     CDS             1540..>2432
                     /locus_tag="JQG95_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY5890491.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT          629 a          578 c          603 g          622 t
ORIGIN      
        1 tactcgtgag gatttatgtt ctgctggcag tttcgtgatc aacacctcgc gccaggatgg
       61 ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt tcattgtttt tatcgtcggc
      121 atggggatgt cggctttccg cacctgaaca taatatttac cagaaacagg gaaaaggcgt
      181 ggtgtacctg cgcccttata aagaaacaaa tctttctcta cctgacgtga attacaagag
      241 tttgcgtcgg ttacccaatt tattaataga cccgacaaca ctagacgaat gggataaaga
      301 accgcctctg actgatttga caaccgacta cctctatgag ggcgcacagg cctggtatcc
      361 tcattattca tggcatagcg atggtcgcaa tatcctctac gcgggtgaag ttgtgcaaaa
      421 ccctcccggc acacctcccg tcgatgtcgc ctcgttcaag gcatggggtg attttgccgc
      481 agataaacac agtctctatt ttgaaggcaa gcgcaccgat gacaatggtg gtggaaatag
      541 cttggatatc aaaacgctgc atcaggtgga attccggcct ccctgggatc ctgacttact
      601 ggggctgata ttacgtgacg ctaatttctt gtatatcaat gggcatcgcc tcgctgaccc
      661 cgagagtttc cgtgtgctgg cgcagaagtc ctgggatcag cgaggaaagt tttccaccac
      721 gtttaacccc tgcattgccg taccatttgg cccctgggat accctggctc gtacacggac
      781 caaaatcctg ctcaacggcg aacagcttga tgccgacccg gacacctttt ccgtcgtgcg
      841 ctggatgccc ggctcactct tgacctggcg tgataaaaac gggctgcagc gtaaagtcct
      901 cgacaaggaa aatctggcgt gggatgaaga tttaacaaag cactgtctgg atttttctct
      961 gctggaaaag aaagtgttct ggcgtaaagg acctgcttgt aaacaggaag aattacccgg
     1021 actcgatccg gagcagtttc aacccatcag tgatgctgtc gcccagcatc aggactcgct
     1081 ttataccatc atcgaaacag agtctggtga ccgtaagctg gagatcgtga aacttgatga
     1141 tcccaatctt attatcaaca aacgtttcaa cgccgggaaa cgccacggct atttacttac
     1201 gcgtgccgaa gggtagccat accattccgg tttacacgtg tttgaatctg acggaccgct
     1261 gatcttactg gataaccgtt ctccggatga acgcgaagcc catcttaatg accatccctt
     1321 tttgcgcaga tggtatgccc gcgataaccg ctacatttac agctttgatg gcgcgcagct
     1381 ctggcgatac cgcaccgctg atccgaaaca agttcgctta atctggaagg aacaacattc
     1441 gggatatgtc tatggcgtaa attacaaaac gggatatctg gacggaaaaa ttactgatga
     1501 cggcgaattt attcctgccc cgcgcaatga ggcgacaaaa tgaagggtca aataatcaca
     1561 gttgcactca tcctgttggg acacacattt ctgacgcctg ctgtacaggc catagggttc
     1621 gactattaca atgatcacgg tgttatgtct tacggtaaag gttattgggg ggataagaaa
     1681 atcattgccc agtttccgga gatgagcagg gccggtttgc gccttgtgaa aaatatctct
     1741 gggaaaacgg gttatatacc ttccgatgaa ataagcatag aaaatgagga ataccactgg
     1801 gtgacagacg gccgcgttat tctctggcgt ggcaaaatcg ttagcaatcc accgggaaca
     1861 ctcactgtcg atattgccag ctttcaggct atgggccgct tcgcggtcga taaatatagc
     1921 ctctattttg acggacagcg caccgaaagt aatagcggcg cgtcacgtgt tgagctggcg
     1981 acactaaaag ctatcgaagg taactctacc acgctggtgg atagtaaaaa tctctacttg
     2041 tccggtcgac gtcaggggag cagcagtgat gttactgtat tagaaaaaag atggtggggt
     2101 attaatccac gtcttatgag tgtaaacaga aattcatatt ccaatgatct gcttatccgc
     2161 agtgggcaga atatttattt aaatggcgtt caccttacgg cgaatgcaga ctcatttgag
     2221 ataattcgct ggatacctca ttcactgctg gtttttcgcg acaataaggg tctgcatcgt
     2281 tatccctttg gtcaattatc aggcaaagcg atacccgtag atgatgacgt ctcttttgaa
     2341 gtaggggaaa gtcgcgttcg ctggcgtaaa cagctcacgc ccgaccgtca gtggagcaag
     2401 tggatagacc taccaggtat tgaacctgaa ca
//
LOCUS       DADLCQ010000256         2390 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000256 DADLCQ010000000
VERSION     DADLCQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.259"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            100..1059
                     /locus_tag="JQG95_004152"
     CDS             100..1059
                     /locus_tag="JQG95_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5890492.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1056..2201
                     /gene="ssuD"
                     /locus_tag="JQG95_004153"
     CDS             1056..2201
                     /gene="ssuD"
                     /locus_tag="JQG95_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY5890493.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2210..>2390
                     /locus_tag="JQG95_004154"
     CDS             2210..>2390
                     /locus_tag="JQG95_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HAY5890494.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          527 a          653 c          673 g          537 t
ORIGIN      
        1 tgcaaaccca tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
       61 ttcaggttcc tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg
      121 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      181 gcgttacgta taggctatca gaaaggcagt attggtatgg tgctggcaaa aagccaccag
      241 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      301 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
      361 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
      421 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
      481 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
      541 ctgcgtcagg ccggacttaa attcaccgat atccagccca cttacctgac gcctgccgat
      601 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
      661 tccgctgcgt tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa
      721 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
      781 ggcgtactgg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc
      841 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
      901 catcgccctc ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acagcagcaa
      961 acggcagatc tgttttatga aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1021 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1081 taccgaccca cggtgacggg cactatctgg gaacggaaga aggttcacgc ccggttgatc
     1141 acggttatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1201 tcccaacggg gcgatcgtgt gaagatgcgt ggctggttgc agcgtcgatg atcccggtga
     1261 cgcagcggct gaagtttctt gtcgccctgc gtcccagcgt aacctcacct actgttgccg
     1321 cccgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     1381 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     1441 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     1501 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     1561 agccttatcc tccgctttac tttggcggat cgtcagatgt cgcccaggag ctggcggcag
     1621 aacaagttga tctctacctc acctggggcg aaccgccgga acttgttaaa gagaaaatcg
     1681 aacaagtgcg ggcgaaagct gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc
     1741 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     1801 atcttgatga tgagactatc gccaaagcac aagccgcatt cgcccggacg gattccgtag
     1861 gacaacagcg aatggcggcg ctacataacg gcaagcgcga caatctggag atcagcccca
     1921 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     1981 gtcctacggt cgccgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2041 tttcgggcta cccgcatctg gaagaagcgt atcgggttgg cgagttgctg ttcccacatc
     2101 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2161 tggcaaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaacacga tgatggcaac
     2221 gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg gcatcgtggc
     2281 ggtgtggcaa ctggcctcct cggttggctg gctgtcgacg cgtattttgc cttcaccgga
     2341 aggagtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
//
LOCUS       DADLCQ010000257         2347 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000257 DADLCQ010000000
VERSION     DADLCQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.260"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..797)
                     /locus_tag="JQG95_004155"
     CDS             complement(<1..797)
                     /locus_tag="JQG95_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890495.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1398..1646)
                     /locus_tag="JQG95_004156"
     CDS             complement(1398..1646)
                     /locus_tag="JQG95_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY5890496.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2001..>2347)
                     /locus_tag="JQG95_004157"
     CDS             complement(2001..>2347)
                     /locus_tag="JQG95_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY5890497.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          606 a          431 c          565 g          745 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggtaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag gtaattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggcg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggggcaatt tttccttaag gtatgaaaaa aggagctgaa gctatataat agctattaat
     1081 gccactggta tcagtgaatc atgtatataa aaaactgtgt taaggaggct gtccattagc
     1141 ggttttttat tcttctggaa ccgcggcatc tttgcgtatc tttttgtctt ctcaccgtct
     1201 ggtcggtgtt ttgctgagac tgctaatttc ctgtctttgc tagtgctgta ctggtactgc
     1261 ccaatcatga ttggcggggg cgggcggagt tgagaccgca gacacgtcgt atgcaagaac
     1321 gtgctgcggc tggctggtga acttccgata gtgcgagtat tgaatgattt ccagccgtta
     1381 ctgattttac gtgctaatta gtgaacaaac cactcgtcag cagactccca ggtatctttc
     1441 agagtttcct gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg
     1501 ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt
     1561 cgggttaatt cttcctttaa cgcatccaca gcaccggttg gcattttagt cattttctct
     1621 ttggctatgc agatttcaaa acgcataaaa atccctctgc actgtgtttt tatacagtat
     1681 tatttttaac tgtatggata aacagtgtca agagtgaggg aaagtgattt tttggggcat
     1741 gcatggggca aaaatatttg ttgtggggca ttttggggca tgattgggac actttgttac
     1801 atatgaactt tgccgatttt catatgacat ggcaatccaa cgctttgatt attctaaaga
     1861 aaacacatgc tcttgggcgt tctttagtga tttttaaaaa tcccgtatca cgcagttaaa
     1921 gtggcgggca tactcttcaa ggctggtgat ccccaaccgc acccatttag gatgtgacca
     1981 ttgcggtaga ccgatgtaaa tcataacgcg tgaaggatct cttccacgct acgcacacgg
     2041 gcgatgcgcg ggtagatatg attaatgctg ttattgtgtt gctcggcgct ggcggcacta
     2101 caggcgtctt cggcaatcac cagattaaag ccaagttccc aggcattgcg ggcggtggat
     2161 tcaacaccga tattggtcga gatcccacat aacactattg tatcgatacc tcggcggcgc
     2221 agttgcaact ccagatccgt accgtaaaac gcaccccact gacgtttgat gatttcgata
     2281 tcactgtcag ttgcacctaa tgcagctgga tgttgccacc agttttcggg caacacttta
     2341 gcggggg
//
LOCUS       DADLCQ010000258         2334 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000258 DADLCQ010000000
VERSION     DADLCQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.261"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(395..1849)
                     /locus_tag="JQG95_004158"
     CDS             complement(395..1849)
                     /locus_tag="JQG95_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY5890498.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKVN
                     SGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          676 a          436 c          402 g          820 t
ORIGIN      
        1 cgttttgacg gcttcggctt taaactccgt agagtaacgt tgggtttttc tgctcattat
       61 tggctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc
      121 actcccgtaa agacaggagt caagttcccg ctccgacata actgcgtgca atggattttg
      181 aaggaaccgt cagcccattc cccggaggca aacatgggct ggcaaaagtg tagcggtatt
      241 aggcacagct atttaggaaa gagggaccat ctccatctca ttaaatggac cacgccagac
      301 aattcactgt catgctgatg ctgcactctg gggacagaat cactcatgat gacttcgata
      361 atcgacgaca ttattatttg gccgagcttt taggtcatat ctcaaatcgt ttgcctaata
      421 ttgctccatt ttttaataaa aaatcaatca tttttgaatc tttgcttttc attgcattgt
      481 caagcatagt gtccccacta tttacttttt gaaatttttt gtttacatca accaatccat
      541 tattgataaa atattctagc actttatagt tggcaccatc tttactaagt aagtattcaa
      601 catcatacag attacaatat ctattgtttt tcattttctc catctctgcg acatcttgtt
      661 ttgtaaaatt aaatttagat agtaaataaa gtgctatatc agctttttta ttgcctaaag
      721 aataagcaag ggcctgatga gccatttttt tatcagtcac tttattgtta atctcttcat
      781 aattagatgc tttaatcgca tcctccaaag atatgtctct gagttttatt tttttgaagt
      841 tatctgtact tagcattctt ggtatatgat actcaagctg aaacacaaag tttataattc
      901 tatccagact aacaggatca atatttttgt tataacaaaa ctctctgaat ccttgatctt
      961 tactctttct tataaaatca ataagatgca acccaattcc aagcctcata tctttagtgt
     1021 taaatattgg aacatcagtt ttcccctgat taccatgcac atacctgcta acttcatcca
     1081 caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa acaggtggaa
     1141 tagcattatc aaagtgatcg gttaatgtca tatatccata tggagagtca tgatcaatga
     1201 tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg agtttttctt
     1261 gatgattgga aatttcgacc ccaaaaaaaa caaaatcgtg gttagagatt tctttaatat
     1321 cctcagagta tgtattctta tgtgtttgta tattttcaga ctgaatttta tcaaaggatt
     1381 taatattaag aatattattt gacagattag agttactttg atgtctgaag ttaaaattag
     1441 aattaataat aatagaaagt aagtgacgtt cgtctgaaga aagaggatga actttttgta
     1501 ttatctcaga gatatagtgc ctggacacct tatcgagatg ctcaagtgac cgaaatgatg
     1561 accgtgctcc agatggcgct tcaatagaaa gatttagtga tttgaaactg gtcccttgag
     1621 aaaacatttt gctataactt tgcgctgcta tagacttcct taaaaagttt ttcacagtat
     1681 tagcacagtg atctaaatgt ttttcactta tgccagaaga agatgagttt gttacgcgta
     1741 atacattttt ttgtaatgct tttgttgctt tattcggatt cgtttttcca tcaacaagat
     1801 caatattatt ctgaacatta atatgattta ctgcttcagg tattttcatc ccccccccca
     1861 attatgtgcg tttttatctg tggttattta gataacatta tctaactgga cactttcttg
     1921 tatgtattat agctaaatga acagatttta atatgttgta tggttttcac tctgaaatcc
     1981 cctgaattta atcacgtttc ctttactgaa ggcaatttta accgtagtat caattattaa
     2041 cccttgggct tgatatccct ctcctcttct gggcacgggg gatgtcagat tacttattac
     2101 agaaaccaaa agaattgtaa gcgtcatctc agtgtcgtct atttcaatgt ctgatgattg
     2161 gcgatgatag agtcctctta taacgttaat ggactctatc aatgtcagat tctcttcgcc
     2221 cccgcaggat tcgtgcctct tactctatga ctctatggag tttaagcttg atcttattga
     2281 aaaatcttac cagctagggg cctgcgtggc ccaactggcc agagagtacg ggat
//
LOCUS       DADLCQ010000259         2325 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000259 DADLCQ010000000
VERSION     DADLCQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.262"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="JQG95_004159"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="JQG95_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HAY5890499.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="JQG95_004160"
     CDS             276..452
                     /locus_tag="JQG95_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890500.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="JQG95_004161"
     CDS             527..802
                     /locus_tag="JQG95_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890501.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="JQG95_004162"
     CDS             902..>2325
                     /locus_tag="JQG95_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY5890502.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          719 a          531 c          529 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DADLCQ010000260         2252 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000260 DADLCQ010000000
VERSION     DADLCQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.263"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..137
                     /locus_tag="JQG95_004163"
     CDS             <1..137
                     /locus_tag="JQG95_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY5890503.1"
                     /translation="ARKAISVTERQRYILRIRTLTKAVAEAYYASREALGFPMCNKDK
                     "
     gene            147..2216
                     /gene="glyS"
                     /locus_tag="JQG95_004164"
     CDS             147..2216
                     /gene="glyS"
                     /locus_tag="JQG95_004164"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY5890504.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT          528 a          598 c          654 g          472 t
ORIGIN      
        1 atgcgcgtaa agccatctcc gtcaccgagc gtcagcgcta cattctgcgc attcgcaccc
       61 tgaccaaagc agtggcagaa gcatattacg cttcccgtga agccctcggc ttcccgatgt
      121 gcaacaaaga taagtaagag gcggctatgt ctgagaaaac ttttctggtg gaaatcggca
      181 ctgaagagct gccaccaaaa gcactgcgca gcctggcaga gtcctttgct gcgaacttta
      241 ctgcggagct ggataacgca ggcctcgcac acggcaccgt tcaatggttt gctgctccgc
      301 gtcgtctggc gctgaaagta gctaacctgg cggaagcaca accagatcgt gaaatcgaaa
      361 aacgcggccc ggcgattgcc caggcgttcg acgctgaagg aaaaccgagc aaagcggcag
      421 aaggttgggc gcgtggttgt ggtattaccg ttgaccaggc tgagcgtctg actaccgata
      481 aaggcgaatg gctgctgtat cgcgcccatg tgaagggcga aagcaccgaa gcactgctgc
      541 cgaatatggt tgcgacttcg ctggcgaaac tgccgatccc gaaactgatg cgctggggtg
      601 caagcgacgt gcacttcgtg cgtccggtgc acaccgtgac cctgctgctg ggcgacaaag
      661 tcattccggc aaccattctg ggcattcagt ccgatcgcgt gattcgcggc caccgcttta
      721 tgggcgaacc ggaattcacc atcgacaacg ccgatcagta tccggaaatt ctgcgtgagc
      781 gcgggaaagt catcgccgat tacgaagaac gtaaagcgaa gattaaagcc gatgccgaag
      841 aagcagcgcg taagattggc ggtaacgctg acttaagcga aagcctgctg gaagaagtgg
      901 cttcgctggt ggagtggccg gtcgttctga ccgcaaaatt cgaagagaaa ttcctcgcgg
      961 tgccggctga agccctggtt tacaccatga aaggtgacca gaagtacttc ccggtgtatg
     1021 cgaacgacgg caaactgctg ccgaacttta tcttcgttgc caacatcgaa tcgaaagatc
     1081 cgcagcaaat tatctctggt aacgagaaag tcgttcgtcc acgtctggcg gatgccgagt
     1141 tcttcttcaa caccgaccgc aaaaaacgtc tggaagataa cctgccgcgc ctgcaaaccg
     1201 tgctgttcca gcaacagctg ggtacgctgc gtgacaagac tgaccgcatc caggcgctgg
     1261 ctggctggat cgccgaacag attggcgctg acgttaacca cgcaacccgt gcgggcctgc
     1321 tgtctaagtg tgacctgatg accaacatgg tcttcgagtt caccgacacc caaggcgtta
     1381 tggggatgca ctacgcgcgt cacgatggcg aagcggaaga cgtagccgtc gcgctgaatg
     1441 agcagtatca gccgcgcttt gccggtgatg acctgccgtc caacccggta gcttgtgcgc
     1501 tggcgattgc tgataagatg gatactctgg cgggtatctt cggtatcggt cagcatccga
     1561 aaggcgacaa agacccgttt gcgctgcgtc gtgccgcact tggcgtgctg cgaattatcg
     1621 ttgagaagaa cctcaacctt gatctgcaaa cgctgaccga agaagcggtg cgtctgtatg
     1681 gcgataagct gactaatgcc aacgtagttg atgatgttat cgactttatg ctcggtcgct
     1741 tccgcgcctg gtatcaggac gaaggttata ccgttgacac catccaggcg gtactggcgc
     1801 gtcgtccgac tcgtccggct gatttcgatg cccgcatgaa agcggtatcg cacttccgta
     1861 ccctggaagc agctgcagca ctggcggcgg cgaacaaacg tgtatctaac atcctggcga
     1921 aatctgacga agtgctgagc gatcgcgtga atgcctctac cctgaaagag ccggaagaaa
     1981 ttaaactggc gatgcaggtt gtggtgctac gtgacaagct ggagccgtac tttgcggaag
     2041 gtcgttacca ggatgcgctg gtcgaactgg cagagctgcg tgagccggtt gatgctttct
     2101 tcgataaagt gatggtcatg gttgatgaca aagaattgcg tctcaaccgt ctgaccatgc
     2161 tggagaaact gcgcgaactg ttcctgcgcg ttgcggatat ttcgctgttg cagtaataac
     2221 gccgttatta aaaagcctgc catctggcag gc
//
LOCUS       DADLCQ010000261         2201 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000261 DADLCQ010000000
VERSION     DADLCQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.264"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            19..2201
                     /locus_tag="JQG95_004165"
                     /pseudo
     CDS             19..2201
                     /locus_tag="JQG95_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          586 a          490 c          553 g          572 t
ORIGIN      
        1 gataaggata ggcgtattat gatgacgacc agaatagtgg ttggcctcac ggcagggacg
       61 tgtctgattt tctcgcaaaa cctgatggcc gaggtcagtg tattcaatcc ggcgcttctg
      121 gaaatcaacc atcaatccgg agtcgatatt cgccagttta atcgggcaaa cctgatgccc
      181 ccaggtgttt atagcgttga tatttttatc aacggtaaaa tgtttgaacg tcaggatgtg
      241 acatttgttc aggataatcc agatgctgat ctgcacgctt gctttattgc cattaaaaaa
      301 acactgtcct cctttggcat aaaagttgat gcgctcaaat cgttcaatga tgtggatgag
      361 acggtttgcc tcgatcctgc tccacgtatt gaaggctcat cctggcagtt tgacagtgat
      421 aaattgcagc tgaatatatc cattcaccaa atctacatgg acgcgatggc ttatgattac
      481 atcagcccca cgcgttggga tgaggggatt aatgcgctca ccatcaacta cgatttttct
      541 ggttcacata cactacgttc agattatggt tcacaagaga cagataccag ttatctcaat
      601 ctgcgcaatg gactgaatat tggaccgtgg cggctacgta attacagtac tttaaacacc
      661 agcgatggcc gtgcggaata caactccatt agtacctgga tacagcgcga tattgccgcg
      721 ttaagaagcc agattatgat tggtgatacg tggacggcga gcgatatttt cgacagtacg
      781 caaattcgcg gcgcgcgttt gtatactgat aacgatatgc tacccgccag ccagaatggc
      841 tttgctcctg tggttcgtgg gattgcaaag tccaacgcca ccgtcatcat tcggcagaat
      901 ggctacgtga tttatcagtc agccgttcca caaggtgctt ttgagatcac cgatctcaac
      961 accgcaagta caggtggcga tttggacgta accatcaaag aagaagacgg tagcgaacaa
     1021 cgattcaccc aaccttatgc ttcattggcg attcttaaac gtgaaggtct gacagatgtt
     1081 gatgtcagcg tgggtgaatt gcgcgatgaa gacggattta caccggacgt ccttcaggcg
     1141 caaatacttc atggtttttc ccacgggatc actttatatg gaggtatgca ggctgctgaa
     1201 aattatggtt ctgcagctct gggtgtcggt aaagatcttg gcgctttggg cgcaatttct
     1261 ttcgatgtga cacatgctcg tgcgaatttt agccatgatg atacagaaac gggtcagtca
     1321 tatcgctttc tctattcaaa actatttgac gacacagaca ctagcttgcg cctggttggc
     1381 tatcgttact ccaccgaggg ctactatacc actcaatgag tgggcatcgc ggcgcaacag
     1441 ccctgaagac ttttgggaaa caggtaaccg acgtagtcgc gtggagggaa cgctaacgca
     1501 gtcgttgggg agagattatg gcaatttata cctgacatta agccggcaac aatactggca
     1561 taccgatgat gtcgaacgat taatgcaatt tggctacagc agtagctgga agcgtctctc
     1621 gtggaacgtc tcctggagtt attccaatac tgccagacag gggacgggga acaaccatgc
     1681 cagtgataac accagtgagc agatctacat gctctcttta tctgttcctt tatcgggctg
     1741 gtggggtaat agttacgcca cctattctgt ttcgcaaaac gataattccg gtagctcaca
     1801 tcaactcgga ctcagcggta cggcgctgga aagaaataac ctttcatgga atttaatgca
     1861 gtcctataac agtcatgatg atgaggttgg cggtaatatg tccctgacct atgatggctc
     1921 ttatggcacg gtgaacggca gctataacta cagccaaaat tcccagaggc tgaattatgg
     1981 tatcagaggg ggaattctgg cacacagcga aggggtaacg ttaagtcagg agttaggtga
     2041 aactattgct cttgttaaag cacctggggc cgccgggtta gaaatagata atatgcgcgg
     2101 tgctgcgacg gactggcgcg gctatacggt caagacacag ctaaaccctt atgatgaaaa
     2161 tcgggtagca atcagcgata actatttctc gaagtcgaat a
//
LOCUS       DADLCQ010000262         2187 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000262 DADLCQ010000000
VERSION     DADLCQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.265"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            231..698
                     /locus_tag="JQG95_004166"
     CDS             231..698
                     /locus_tag="JQG95_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890505.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGR
                     YYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            700..1305
                     /locus_tag="JQG95_004167"
     CDS             700..1305
                     /locus_tag="JQG95_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY5890506.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSCWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1292..>2187
                     /locus_tag="JQG95_004168"
     CDS             1292..>2187
                     /locus_tag="JQG95_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890507.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          532 a          507 c          610 g          538 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtcagtat ccattaacac ccggttcgct ttatgttggc gtgtacggcg
      241 gcagcccggc agatatcgcg gcggccatct ggcgtaaggc tccgccgggt attgatatga
      301 atggcaacac aacgttcact gttgcagata aggagtacga tccgccgtat cctgaatacg
      361 tgatcagctg gcagacactc aaacccgtca gtctgcatgt cagtgtgacg ctgaaaaaaa
      421 gtgactacct gccttcagat attacccgac aggtacagca atccgtgctg gacgcattta
      481 atggtacaga tggtggtctg cgggcaagag ttgcctctgt tgtctctgcc gggcgctact
      541 atgccggcat ttacaaaacc gatccggaac atattgatat tctgggcctt actgtgagcc
      601 gtgatggttc gtcatggaca acggctgtca ctttcgggat agatgagatt ccggttctgg
      661 atgtgtcgga tatcagtgtg aaactccagg aggcataacg tgcagaatgt ggctgccact
      721 gtgcttgcgc agtatgctgc cagcccccga ctcaatgccc tcattaacag ctttaacgca
      781 gcgctttccc ccgacagttt tatcagtgat ttttatggtc ttatctggaa catcgatacc
      841 gcagaaaagt atggtcttga tgtctgggga aagattgtgg gtgtcagttg ctggctgacg
      901 gtaaaggacg attttaatta cctgggcttc agcgagtcca ggatggacac cccggtaatg
      961 gatgatccct gtccgtttaa tcaggcaccg ttttacaacg gaaaatcgga tacccggact
     1021 gttgacctgt ctgatgctgt ataccggcgg ctgatactga tgaaagccat gtcgaacatt
     1081 actgactgtt ccgttccgga tattaacctg atgctgagat ttatgttcgg aaaaaaacgc
     1141 cgggcttatg ttctgaataa tggcgggctg aggatgagtt acgtctttga gtccgcgctc
     1201 tcgttggcag aactggcgat tatccagtcg tcgggtgcac tgccatcccc gccgggtgtt
     1261 tatgtttcag tagttttaaa ggagtcccgt aatgaaggcc agtgataaac cccgccagct
     1321 ggcggtcccc tttgcgagta ccggagataa aaaccgtatc ccggacaagg cgacacagca
     1381 gaccagagag agcggtaatg ctgcgtatga ttcaggtttt cctccggtga ccatgacagc
     1441 ggtctcagcg ggaggtatac cgccacacgg caaggatttt aacggtctga tgtacgatat
     1501 taccgcagca atacggttcg cccaggctgg cggtttgtac acgtataatg ccggttttgc
     1561 gggggccatt ggtggatatg caaaaggagc cattctcgcc ggagtcgcaa caacagcggt
     1621 ctggctgaat accacggacg ataacctgac cgatcctgaa ggctccgaca gtgcgggctg
     1681 ggtaaatctt cttgaggatc cgaaaaggat attcctgcgg cagaagaaca atctgtcaga
     1741 ccttcagaat aaagggacgg cacgggataa tcttcaggtt tacagtaaag agcagtcaga
     1801 tcaacgctat gttcatcggg aaggcgataa aataaccgga gagctgaaaa tccgtggtgt
     1861 taatgcgctg aggattttca acgaagcttt tggcctgatt tttcgtcgtt cggaagagtg
     1921 cctgcacctt attcccacca gtgaaggtca gggggaaaat ggcgatattg gtcccctgcg
     1981 cccgttcacc attaatttgc ggacgggtga aatatccatg tcgcataaag tgtctgttgg
     2041 cggtggttcg caggtcaatg gtgcgctggg tatcggcgtt cagaacgccc tgggggggaa
     2101 ttcaattgtt cttggtgata atgacaccgg atttaaacag aatggagacg gtattctgga
     2161 tgtttatgct aatagtcagc gtgtatt
//
LOCUS       DADLCQ010000263         2187 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000263 DADLCQ010000000
VERSION     DADLCQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.266"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(220..384)
                     /locus_tag="JQG95_004169"
     CDS             complement(220..384)
                     /locus_tag="JQG95_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890508.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="JQG95_004170"
     CDS             1286..1570
                     /locus_tag="JQG95_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5890509.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="JQG95_004171"
     CDS             1558..2043
                     /locus_tag="JQG95_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5890510.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DADLCQ010000264         2182 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000264 DADLCQ010000000
VERSION     DADLCQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.267"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1971
                     /locus_tag="JQG95_004172"
     CDS             <1..1971
                     /locus_tag="JQG95_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY5890511.1"
                     /translation="DQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLI
                     NRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFP
                     DIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
                     VPVLNTANIRDGELRRLSTWEKTPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRL
                     ISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSD
                     GQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYV
                     ALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELR
                     DVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDD
                     GNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIA
                     GEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRAD
                     EIVRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPESVLREPQRVREAVR
                     EIARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            1991..>2182
                     /locus_tag="JQG95_004173"
     CDS             1991..>2182
                     /locus_tag="JQG95_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890512.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAG"
BASE COUNT          506 a          557 c          749 g          370 t
ORIGIN      
        1 gaccagctgc aggccatcgc gcccggtcag cctttccgtc tccagcagac gcgcagtgct
       61 gccgatgtgg tcatcatgaa ggagattgtg cgtcagacgc cggaactgcg ggaggcggta
      121 tacagcctga ttaaccggga tgtggaaagg gcactgtccg ggcttgagag tgtgaaaccg
      181 tctcaggttc cccgtcagga gggcgcatgg gcaccggagc actccgtgac ggagttcagt
      241 cacagccagg aagcgaaact ggcagaagcg cagcagaagg cgatgctgaa aggcgaggct
      301 ttcccggata tccccatgac actgtatgag gccattgtcc gcgactatac cggcaggaca
      361 ccggaagcac gggagcagac gctgattgtc acgcacctga atgaggaccg gcgcgtactg
      421 aacagcatga ttcatgatgc acgggaaaag gccggtgagc tgggcaaaga gcaggtcatg
      481 gtgcctgtcc tgaacacagc gaatatacgt gacggagaac tgcgtcgtct ctccacctgg
      541 gagaaaaccc cggatgccct tgccctggtg gatagtgtgt atcaccggat tgccggtatc
      601 agcaaggatg acgggctgat aaccctgcag gatgcggaag gtaacacgcg gctgatttcg
      661 ccccgggagg cggtggctga aggtgtcaca ctgtacacac cagacaaaat ccgggtgggg
      721 accggcgacc ggatgcgctt cacgaagagt gaccgggagc gcggttatgt ggccaacagc
      781 gtctggacgg tgacagcggt ttccggtgac agtgtcacgc tatcggacgg acagcagacc
      841 cgggtgattc gccccggcca ggagcgggca gagcaacata ttgacctggc ctatgccatc
      901 actgcccacg gtgcgcaggg ggcgagtgaa acctttgcca tcgcgcttga aggcacggaa
      961 ggtaaccgga aacagatggc cggctttgag tcagcctacg tggccctgtc gcgtatgaag
     1021 cagcatgtgc aggtgtacac cgataaccgt cagggctgga cggatgccat taacaatgcc
     1081 gtacagaaag gaactgccca cgatgtgctg gagccgaaac cggaccggga ggtcatgaat
     1141 gcgcagcggc tgttcagtac ggcgcgggaa ctgcgggacg tggcggcagg gcgtgctgtt
     1201 ctccgtcagg cggggctggc cgggggagac agtccggcac ggtttattgc accggggcgc
     1261 aaatatccgc agccgtatgt ggcactgccg gcgtttgacc gtaacggcaa gtcagcaggt
     1321 atctggctga acccactgac cacggatgac ggaaacgggc tgcggggatt cagtggtgaa
     1381 ggacgggtga aaggcagcgg ggatgcgcag ttcgtggccc tgcagggcag ccgtaacgga
     1441 gagagcctgc tggctgataa tatgcaggat ggtgtccgga ttgcccgtga taatcctgac
     1501 agcggtgtgg tggtgagaat cgccggtgaa ggtcgtccgt ggaatcccgg tgccatcacc
     1561 ggtggtcgcg tgtgggggga tatcccggac aacagtgtcc agccgggagc cggaaatggc
     1621 gaaccggtca cggcagaggt gctggcacag cggcaggctg aagaggccat ccgtcgtgaa
     1681 acggaacgcc gcgcagatga aattgtccgt aaaatggcag agaacaaacc tgacctgccg
     1741 gatggcaaaa cagagcaggc tgtcagggag attgccgggc aggagcgtga ccgggctgcc
     1801 ataactgaac gggaagccgc gctgccggag agtgtgctgc gtgaacctca acgggtgcgg
     1861 gaagctgtca gggaaattgc ccgggaaaat ctgctgcagg agcgacttca gcagatggag
     1921 cgggatatgg tccgtgacct gcagaaagag aaaaccctgg gcggagactg atacaggaag
     1981 ataaacgctg atgacaacgg ataacacgaa cacgacacgt aacgattcac tggctgcacg
     2041 gaccgatacc tggtttcagt catttctggt ctggtcaccc ggacagcggg atatcatcaa
     2101 aacggtggca ctggtgctga tggtactgga ccacatcaat ctgatattcc agctgaagca
     2161 ggaatggatg tttctggccg gg
//
LOCUS       DADLCQ010000265         2147 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000265 DADLCQ010000000
VERSION     DADLCQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.268"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..1273)
                     /locus_tag="JQG95_004174"
                     /pseudo
     CDS             complement(1..1273)
                     /locus_tag="JQG95_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
     gene            1355..1582
                     /gene="vapB"
                     /locus_tag="JQG95_004175"
     CDS             1355..1582
                     /gene="vapB"
                     /locus_tag="JQG95_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY5890513.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            1582..1980
                     /gene="vapC"
                     /locus_tag="JQG95_004176"
     CDS             1582..1980
                     /gene="vapC"
                     /locus_tag="JQG95_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY5890514.1"
                     /translation="MLKFILDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1989..>2147)
                     /locus_tag="JQG95_004177"
     CDS             complement(1989..>2147)
                     /locus_tag="JQG95_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY5890515.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
BASE COUNT          477 a          638 c          532 g          500 t
ORIGIN      
        1 gccagaacac tgacggcatc gctgtaaccg gccgtccggg ggacactttt ctccggatgt
       61 acggtcaccc ggttctgttt catgatgtca cgactgagtg cccggactga cagctcatcg
      121 agcatatgaa tcccgaacgt gaacagcccc ttctcacgat cgagggggat aagctgctca
      181 cggctgatgg cctcatcgat accggcgcgt gcccgttcaa tcacaccatt ttccggcggc
      241 agtatgccga ccgtcctggc cagcaggtcc gtatacataa actgcacttt gcgttcactt
      301 aatccggcaa tggcctgtgt caccgcctgc tgcacatccg gcccgtcctg tgaagcgggt
      361 ccgggtgtct gcgtgcgggt atatgcccgt tgttccgctg cgtcacgata tgcccggatg
      421 tcgaacccgg tttccttcag cgtctgcatc cactcggcca ttctgacttc aggatcgacg
      481 tgctgtttgg atttacgcgt atccagcgcc gccacatccc gggatttcag cgaggcgtct
      541 tccccgacgg cctcccggat agtctgtgag cgtccggaaa aggcctccac cggtacaccc
      601 ggcatttccc acataccgtg cttaccgacc acttcagttt catagcccag cgcctcaacc
      661 tgctctttca gtttttcccg gtagagcctg ccaaaggcaa tctgattagc gtacacattc
      721 tcaatgaacc ccgttttccc cactttgtca ctgctcagtg tcttccattc gccgttatac
      781 tgcgtaacat tagtcaccac cgcatgcgtg tgtaactgtg gttcctgatc gcgactggtg
      841 tcgtggttaa acagtgccat caccagatta ccggtcagca ccgtttctga ctgtccgtcc
      901 gtcaggtgga ggccagtgcc tccacctgac ggacagcaaa atccacggcc tggttatgtg
      961 cttcaataag acgtttgtcc cccccgagca tggccatcat ggagatactt ttgggagcgg
     1021 agaaggtcag gtcgtagccg ggacgatgcc tgttactgcc atcctgcatg cggcttaaat
     1081 ccgccccgtc cggcagcctg ccctccagaa ggcgggtaaa gacatcctta tcgacactgc
     1141 cctgcagccc cagttgttca gccccctggc cggcccagcg ttctcccatg ctgcccagca
     1201 catagtaatt atccttgtcg gtataataat taccggcact tccggccgat ctgacctgcg
     1261 cgatactcat cataaacgta tatccctttg acatatcccg gtatcaatcc cacaatagat
     1321 atacacaaga catatccaca taaggaggca aataatggaa accaccgtat ttctcagcaa
     1381 ccgcagccag gcggtcagac tgccaaaagc ggttgcattg ccggaaaacg taaaacgcgt
     1441 tgaagtgatt gctgtcggac gaaccagaat cattacgcca gccggagaga catgggacga
     1501 atggttcgac ggacacagcg tcagcaccga ttttatggat aacagggaac agcccggcat
     1561 gcaggaaagg gagtcattct gatgctgaag tttattctcg ataccaacat ctgcattttt
     1621 acgataaaga acaaacccgc cagcgtcagg gagcgtttta acctgaacca ggggaaaatg
     1681 tgtatcagtt cggtcaccct gatggagctg atatatggtg cagaaaaaag ccagatgcct
     1741 gaacgtaatc tcgctgtgat cgagggattt gtttcccgca ttgatgttct ggattacgac
     1801 gctgctgcag ccacacacac cggccagata agagcagaac ttgcccgtca gggacgccct
     1861 gtcgggccat ttgatcaaat gatcgcaggt catgcccgca gtcgggggct gattattgtg
     1921 actaataaca cccgggaatt tgaacgtgtg ggcggcctga gaactgaaga ctggagctga
     1981 cctgttcatc agaaatcatc tcccggctca acatcctccc cgcgttcccg gtgcacattg
     2041 atgttcacct cttcacgacg ctgcatctgc tgccaggtgt ccggatgatt ttcctgctgc
     2101 catgcctcat aagcggccat atccaccact tcaccggatt cactgat
//
LOCUS       DADLCQ010000266         2145 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000266 DADLCQ010000000
VERSION     DADLCQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.269"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(55..321)
                     /gene="ospE2"
                     /locus_tag="JQG95_004178"
     CDS             complement(55..321)
                     /gene="ospE2"
                     /locus_tag="JQG95_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY5890516.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(503..904)
                     /locus_tag="JQG95_004179"
                     /pseudo
     CDS             complement(503..904)
                     /locus_tag="JQG95_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1333..2121
                     /gene="virF"
                     /locus_tag="JQG95_004180"
     CDS             1333..2121
                     /gene="virF"
                     /locus_tag="JQG95_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY5890517.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          660 a          383 c          397 g          705 t
ORIGIN      
        1 caaaccctga tatattcttt tgaaaatgga gcttaaacga gtctgaaatt aacttcagaa
       61 atcagaatac cgttggcttt gtttttcatc aattccggct tcatttatct ttgtcaacca
      121 ttgcatttct gtagcagtga atttcaacgt tttcgtccgg ttggcagcag gtatttgtaa
      181 ttcgtatata gctgcttttt tattgaaaac agtatatcca tctgtagtaa cagtagaaga
      241 taataataca ggatttgata cttcaagaat gatattttct tgtttttgtg gaaggcaagg
      301 gaatatagtt tgtgtaagca tattgcttcc ttatgttata ttttagtaaa gaatttattt
      361 tttacattat agtcggctaa atgaagttta ctgtaaaaaa acgatacttt tttcgtaagc
      421 gatgagatgt ggagccagaa aaatggctgc gttacgtcat tgaactgtcc ttgacccacg
      481 atattcagac agtcgttgta tcttaagttg acattacccg ctgacgtcga tattcccttg
      541 gtgagagata acccaacgca ctatgcggat ggttttcatt gtaatgctcg atcgccactg
      601 caaggttatg caatgccgtt cttggtttcg gcatgaacgc gatgcagtct tccttcatcg
      661 ttttcatgaa ccgttctgct attccattac tctgagggct actgactgcc gtcgtgcagg
      721 gctccagatt caactctctg gcgaactgcc gtgtttcatg agccgtataa gccgaaccgt
      781 tatcggccac tggattggcc gttccggtcc tttatcgcca tatcgctcct gacatgacat
      841 cctgcaccgt tgctttgtca taatctcccg tgcctgctgc ccagtctatg gcttcacgat
      901 cgcaatatgg aacgtaacgc tgtcatcggg agaatcgtca atgcggcatt cggttaacgc
      961 ttaccaatat caacaccaac cggatttgtg gagcctccag tctgaagggg ctttatgcgt
     1021 tccgtatagg atattatgat gctggagttt tgcgaagctc cactcctttc cgaacattta
     1081 cacagatatt gcctaagaaa agtgaaacca taatatggtt atagtccctt tcagtgcaaa
     1141 tacttagctt gttgcacaga gaaatagaag ctgcataagc tctttcttca aaaaatgtaa
     1201 ataaagttaa atataggaaa aattacttaa tctatcttaa taacggaaag attttgtata
     1261 caatcactgt tacagaaatt tcttagttac tctgtaaaca ctaaatatag tttggtatat
     1321 tctgttgaat ttatgatgga tatgggacat aaaaacaaaa tagatataaa ggttcgcttg
     1381 cataactata ttattttata tgcaaaaagg tgttcaatga cggttagctc aggcaatgaa
     1441 actttgacta tcgatgaagg gcaaattgct tttatagagc gaaatataca aataaacgtc
     1501 tccataaaaa aatctgatag cattaatcca tttgagatta taagccttga cagaaattta
     1561 ttattaagca ttattagaat aatggaacca atttattcat ttcaacactc ctattctgag
     1621 gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg aggaggaggt ttctatcgat
     1681 ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa agatctatag tttagcttgc
     1741 cttttatcag ctgtttctga tgaggaagct ttatatactt cgatatcgat agcttcttct
     1801 cttagttttt ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa gagatggcgt
     1861 ctttctgata tttcaaataa cttgaattta tcagaaatag ctgttagaaa acgattggag
     1921 agtgaaaaat taacatttca acaaatcctt cttgatattc gcatgcatca tgcagcaaag
     1981 cttttattga atagtcaaag ctatattaat gatgtatcaa gacttatcgg aatatcaagc
     2041 ccatcttatt ttataaggaa atttaatgaa tattatggta taactccaaa gaaattttac
     2101 ttatatcata aaaaatttta aatgcttcat agcccatcgc tattg
//
LOCUS       DADLCQ010000267         2126 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000267 DADLCQ010000000
VERSION     DADLCQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.270"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            43..222
                     /locus_tag="JQG95_004181"
     CDS             43..222
                     /locus_tag="JQG95_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890518.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            274..468
                     /locus_tag="JQG95_004182"
     CDS             274..468
                     /locus_tag="JQG95_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890519.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1267..1603)
                     /locus_tag="JQG95_004183"
                     /pseudo
     CDS             complement(1267..1603)
                     /locus_tag="JQG95_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          682 a          437 c          436 g          571 t
ORIGIN      
        1 tagcgtaagg cactacagcg cattgcttta cgagtaataa caatggattt atcaccagaa
       61 gacgcaaagt ttttacgaga actgcaacac cgtatgctca gagaagccat tatcgaacag
      121 caaacgggca tgagcgccat agacccattg cgatacaaac gggcctcatc tcctttagtt
      181 gacgacaatt ccaatgcgga taaaacacgt ccaccacact gattacattt ttcattagca
      241 cagtggtgtg atgcagcata cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg
      301 gagcaactgt tacaggcacg ccaacgggag tttgccacta aatggggacg tgccgcgctg
      361 ccacatgaac gcctaaccta ttttccactt gaagacctca ttaaaaataa tggcaaaaaa
      421 atcaggtctg ataagaaaca cttcttagaa tgcaatgaag acaaataaca acaatctgca
      481 tattgccacc aaccacagat gtggcatcgt tatcagcgca cttgattttc tttatattca
      541 ctgacgagct tgccggggca cctttgtttg ccggtcattc tggtatcatg cagatattta
      601 cggcgtacaa gcccactagc atgacattgt aataaggcct gagcctaacg ctttaatgtg
      661 tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt aagcgtccag
      721 cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc aaataagtga
      781 tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat gggagcggat
      841 gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc cgatctgttt
      901 aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca tcgttaatac
      961 gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct gcatatggat
     1021 cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat ggagtagcac
     1081 aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac ttcttcagca
     1141 cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa ctaatacatc
     1201 agaatatgca ttatgttaaa taaatatgaa aagatggttt aaataccccg ttacttttga
     1261 cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt tatttctatt
     1321 agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt ggttatgctt
     1381 taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg tgttaatgtc
     1441 agttcccctt tttaccagtg ctgatttcca cattccgtcc aacagagctt atagcctttc
     1501 cctggattat agcattgtcc ggctgaagtt ctttttgaat aataatagaa gcactgctgg
     1561 cagatccagt ccgtttttca taacccactg tactgataac cataatctaa tcagtagaaa
     1621 ttgagtcgaa aataagcact actccataca ggataattag aggtcagttt gattattcac
     1681 aattcatcat cagcattttc tatttctgac gaaatcaaca tgaaaataac catatatgat
     1741 aattattata ataacggctt taattggaat acatatatta caacgtatta tatataattg
     1801 gtattctggg aactatattc tcaaaatata gtagaaacga ggtatgtttc tggtggaaag
     1861 gacagtggga ttaaaaagta agaattgata aaaaaaacgc cagcaacaca tagtgccggc
     1921 ggagggaata ccccatggag aaaatgtgat gcctagagca tcatgaatat ataattaaaa
     1981 atagttagcg ttgtcaatac tttcacaaaa ataattttcg tagtatagaa agatattttt
     2041 atgcatgacc tacctgaatt tgctccgggt agaggttata aataaaaatt gaatcacgac
     2101 aaacacaata ttcatagtat ggcgat
//
LOCUS       DADLCQ010000268         2112 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000268 DADLCQ010000000
VERSION     DADLCQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.271"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..222)
                     /locus_tag="JQG95_004184"
     CDS             complement(<1..222)
                     /locus_tag="JQG95_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5890520.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLA"
     gene            complement(219..926)
                     /locus_tag="JQG95_004185"
     CDS             complement(219..926)
                     /locus_tag="JQG95_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY5890521.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(923..1747)
                     /gene="dapF"
                     /locus_tag="JQG95_004186"
     CDS             complement(923..1747)
                     /gene="dapF"
                     /locus_tag="JQG95_004186"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY5890522.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(1784..1987)
                     /locus_tag="JQG95_004187"
     CDS             complement(1784..1987)
                     /locus_tag="JQG95_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY5890523.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT          502 a          611 c          531 g          468 t
ORIGIN      
        1 cgccagactt gctgctccca gccctttacg gcgactgcgt acagcaaaat tgcgtaccat
       61 cgctgcatcg cattgttgcc agctttgcag gccgttttcg ctggcaaaat tgatgatcgc
      121 ctcaagctga cgctggtagt taagtagggt tatcgggcta agctgacgct ccacgctcag
      181 ataacgtagg tagcgttcta catcggtgtg taaatcggtc atacgcgttc aatccaacgc
      241 tccagaagct ccggcaacat cagcgcaatt tcatgaagta actgcgttcc ctgcccttgt
      301 tgatagtgac tggcatcgcg actggtaaac agcacaacac ccaaatcagc atcgcttccc
      361 agcatcgaca tcgccaccga tcccaccgct ttcgcttccg gtagcaccac cagcagctcc
      421 ggtccgttaa gcggcccaag atagtgctgt tcctgcccca aacgctgaat acgcagcggt
      481 tcgaaagact gacggcttaa tgccagatga gtgtggttcg acggcgcacc taagcgccag
      541 cgatctggaa acaggcgcag actcgcacca gctaggccga gatcgcgcgc ccagcggtga
      601 aagcgcatca gcatatcgtc gagactgctg gcggcggtga gactgcgctg caggtagagt
      661 aggcgataaa acagcccttc gttggcgata gcctgctcca tcaacaacgc catgttctct
      721 tccagaacat gaatgtgatt acgtgcgcgg gccatgtgcc actcgaccaa cgaaacggtg
      781 ccgcgtaccg gatgcggcac acgtatcgct tctactgcgc gcgcattgcg gataaaaaac
      841 tcaggatttt taatcagata atcgacaacc gcccggtcat caagctccgt gagtgtttcc
      901 tgcagttctt cccctggttg cttcatagat gaataaatcc atcgtagaca tgtaccgccg
      961 ggccagtcat atataacggg tgacccggac ctttccaggc gatatcaaga cgaccgccgg
     1021 ggagttccac gcgtacttct tcgtccagca aaccttgctg aatccctacc gccaccgccg
     1081 cacacgcgcc gctgccgcag gcctgggttt ctcctgcccc acgctcataa acgcgtaaac
     1141 gaatatgctc gcgcttaacc acttgcataa aaccgatatt ggcgcgctcc ggaaaacgct
     1201 cgtgactttc cagaacagga ccaagcgttt ctaccgccgc ggtatcgaca tcatcgacct
     1261 gaatcacgca atgcggattt cccatcgaca ccacgccgca taagattgtc tgctcggcgg
     1321 cgcgcataat ataggtcttt tccgctttgt tagcgcgaaa cggcacggcg gaaggttcga
     1381 agttgggttc gcccatattt acgcggacca gatcatcatc ggtgacggtc agaaccatcc
     1441 gcccgttggc ggtgctgacg cggatatcac gcttattggt cagtcctttc agacgcacaa
     1501 aacgggcaaa gcagcgcgca ccgttaccgc actgcgccac ttcactgcca tcagcattga
     1561 aaatgcgata gtgaaaatcc agttcaggat catacggcgg ctcaaccacc agcagctggt
     1621 caaaccctac ccccaggtgc cgatccgcca ggcgacgaat cagctccggt gaaaaaaaga
     1681 cattctgcgt taccgcgtcg acgaccataa aatcgttgcc aaggccatgc attttcgaga
     1741 actgcatcat ttactccaat cacgcgggta cagaaactga cttttagtaa ttcacctggg
     1801 atggaccatc gccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct
     1861 ctaccggttt ggtcggcggc ggtgcgtttt tatctgcagg cgggaaatag agcggacctt
     1921 tcagaccgca gcccgtcagg ctgaagagag taagtaatac agtgagtgcc ttaaacacgt
     1981 ttttcattat ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa
     2041 ggcgtaaaag caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg
     2101 agtataacgc tt
//
LOCUS       DADLCQ010000269         2096 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000269 DADLCQ010000000
VERSION     DADLCQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.272"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="JQG95_004188"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="JQG95_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY5890524.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /locus_tag="JQG95_004189"
     CDS             1351..2016
                     /locus_tag="JQG95_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY5890525.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMLILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          560 g          515 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgctcatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tttcgt
//
LOCUS       DADLCQ010000270         2085 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000270 DADLCQ010000000
VERSION     DADLCQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.273"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..797
                     /locus_tag="JQG95_004190"
                     /pseudo
     CDS             1..797
                     /locus_tag="JQG95_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1236..2073
                     /locus_tag="JQG95_004191"
                     /pseudo
     CDS             1236..2073
                     /locus_tag="JQG95_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          504 c          631 g          434 t
ORIGIN      
        1 tgggccttgc ccgtagcagt tatttttatc accgggctgc actgaaagcc ggtgataaat
       61 acgccacgat acgtaagatg ctgacagata tctttaacag taattaccag tgttatggct
      121 atcgtcgcct gcatgcgatg ctcaggcatg agggggggcg gctatcagaa aaggttgtac
      181 gcagacttat ggtggaggaa cagcttgtcg tcagtcgtaa ccgtcgtcgt cgctacagct
      241 catattgcgg agaaatcgga ccggctccgg ataaccttat cgccagagat tttaaggcgg
      301 agcaacctaa tcagaaatag ctgacagata tcacggagtt ccagctccct gcaggtaaag
      361 tctggctgtc accagtggtg gactgcttcg atggaaaagt tgtgagctgg tctctcagta
      421 cacgccctga tgctgaactg gtcaacacta tgctggatag cgctgtcgaa acgttaaatg
      481 ctggcgaacg accggtgata cacagtgaca gaggtgggca ttatcgctgg ccaggctggc
      541 tggaaagagt gaatgcagca ggtcttattc gctcaatgtc ccgtaaagga tgttcacctg
      601 ataatgctgc atgcgaaggc tttttcggca gactgaaaac ggaaatgtat tatgggcgta
      661 aatggtcggg catcacgcca gaaaagttca tgcagcaagt ggatgcttac atcagatggt
      721 ataacgagcg gcgtataaaa ttatcgctgg gtgccgtcag ccccaaaatg taccgccaac
      781 aatgcgggct ggaatgataa agcagtccag gaaatcgtcc gcatcccccc ttcaccattt
      841 ggattgtccg ctccacccaa catgttgttt ccttaaggtt cctacaccac aaaggaaact
      901 ctcatgctgc gcagtggatg gctgacgttg ccgacaggcg ggaacttcgc cagttcaggc
      961 agacaccgga acagcgcttc acgcaggaac aggagcatct gcagccgctg ctgggtacgg
     1021 acttcgatat ccgtcatgtc tcctgggatg gctatatcga ggttggcggg aaccgttaca
     1081 gtgtgccgga aagcctgtgt ggtcagctgg tatcaatacg gatctcgctg gatgatgaac
     1141 tgcggatcta cagtaatgag caacaggtag catcgcaccg tctctgttcg gctgcatacg
     1201 gctggcagac cgtgccggag catcacgctc cactctggca gcaggcttcg gagcgcatgg
     1261 ccgaatggct gggggagata cagaaacggg taatcactgt ctgtgaccgg gaagcggata
     1321 tctggcatta cctgtattat aaagtcagcc acggacagcg tggtgcgtgc tgcacagaat
     1381 cgccggctgg aagaggcacc cggcaagctc ttcgaactgc cggaagtcct ggcaaccgca
     1441 ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgtca ggcccggatg
     1501 ttcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca ggcgctcccg
     1561 ctcacgtatg tctgttgccg ggagcaggca gaggacggtg cctgctggca tctgctgacg
     1621 tcaggaaaag cggcgagtgc cgccgatgca cgacgtattg tcagccatta cgagcgacgc
     1681 tggctgacag aggaatacca caaggcgtgg aaaagtggtg gtacatggaa tcgctgcgaa
     1741 tgcagacccg ggataacctt gagcgcatgg tggttatcca ggcgtttata gcggtacggg
     1801 tgctgggcct gcggcaggga ggtgtcagtg aagaaacaca aaatgacagc tgtgagaaaa
     1861 tactgacgcc gacagagtgg aaactactgt gggttaagct ggaaggaaag ccgcttccgg
     1921 tgcaggcacc gaccctgaaa tgggcctggg gcgatggcat gacagcaaac gcacaggtcg
     1981 tcccggttgg ggcgtcatgt gggatggctg gtcagacttc aggatatggt tgagggttac
     2041 ctggtgatga agtctcttga tcaggagctc tgatcaagag acagc
//
LOCUS       DADLCQ010000271         2072 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000271 DADLCQ010000000
VERSION     DADLCQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.274"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..367)
                     /locus_tag="JQG95_004192"
     CDS             complement(<1..367)
                     /locus_tag="JQG95_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY5890526.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFVYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFE"
     gene            complement(406..693)
                     /locus_tag="JQG95_004193"
     CDS             complement(406..693)
                     /locus_tag="JQG95_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890527.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKKIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(943..1155)
                     /locus_tag="JQG95_004194"
     CDS             complement(943..1155)
                     /locus_tag="JQG95_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY5890528.1"
                     /translation="MLCTCRLASYMPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRK"
     gene            complement(1326..1991)
                     /locus_tag="JQG95_004195"
     CDS             complement(1326..1991)
                     /locus_tag="JQG95_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY5890529.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          533 a          533 c          466 g          540 t
ORIGIN      
        1 cctcaaacga ctttagcaat gccgtatcac gggttctgcg tgtcgcaact gtattcagat
       61 attgctctgc cgcttcgctc agagctggcg tatgttcccg gcctactgat tcacacaggt
      121 aatcaacgaa accggacacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      181 tccagtaaac gaaacccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
      241 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc agaaaatcgc
      301 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
      361 gtgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
      421 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatcttttt
      481 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
      541 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
      601 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
      661 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
      721 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
      781 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
      841 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
      901 agaggtgggc ggggatggga tcagctcccc acccggtcac tcttacttcc tggattcgta
      961 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
     1021 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
     1081 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
     1141 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
     1201 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
     1261 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
     1321 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
     1381 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
     1441 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc gccctgcttg
     1501 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
     1561 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
     1621 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
     1681 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
     1741 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
     1801 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
     1861 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
     1921 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
     1981 gcaactgtca taaatatgtt tattgaggtc ataaaaatat caaagagtgt agcattagaa
     2041 cggggctatc ggaaacagat gtgtgactgc tc
//
LOCUS       DADLCQ010000272         2057 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000272 DADLCQ010000000
VERSION     DADLCQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.275"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            6..224
                     /locus_tag="JQG95_004196"
     CDS             6..224
                     /locus_tag="JQG95_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY5890530.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="JQG95_004197"
     CDS             226..591
                     /locus_tag="JQG95_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY5890531.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            588..1253
                     /locus_tag="JQG95_004198"
     CDS             588..1253
                     /locus_tag="JQG95_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890532.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="JQG95_004199"
     CDS             1253..1618
                     /locus_tag="JQG95_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY5890533.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          562 a          427 c          547 g          521 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggc
//
LOCUS       DADLCQ010000273         2048 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000273 DADLCQ010000000
VERSION     DADLCQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.276"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(59..328)
                     /locus_tag="JQG95_004200"
     CDS             complement(59..328)
                     /locus_tag="JQG95_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5890534.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            385..1053
                     /locus_tag="JQG95_004201"
     CDS             385..1053
                     /locus_tag="JQG95_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY5890535.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1369..>2048
                     /locus_tag="JQG95_004202"
     CDS             1369..>2048
                     /locus_tag="JQG95_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890536.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          381 g          604 t
ORIGIN      
        1 ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt
       61 acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt
      121 tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga
      181 tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga
      241 actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta
      301 tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat
      361 ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg
      421 gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca
      481 aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat
      541 ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct
      601 gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca
      661 agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct
      721 gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct
      781 ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat
      841 ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa
      901 aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa
      961 ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact
     1021 gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta
     1081 agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca
     1141 ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata
     1201 gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac
     1261 atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat
     1321 tattccccag tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta
     1381 aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat
     1441 gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa
     1501 aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg
     1561 gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt
     1621 aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg
     1681 aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata
     1741 aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt
     1801 aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt
     1861 gatgcagcca gaaatgcttt ggaagtaata ccagattttc cagaaagaga tgatcatatt
     1921 ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc
     1981 ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa
     2041 actttgtt
//
LOCUS       DADLCQ010000274         2033 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000274 DADLCQ010000000
VERSION     DADLCQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.277"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..363)
                     /locus_tag="JQG95_004203"
     CDS             complement(<1..363)
                     /locus_tag="JQG95_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890537.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..1644)
                     /locus_tag="JQG95_004204"
     CDS             complement(430..1644)
                     /locus_tag="JQG95_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY5890538.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          493 c          412 g          494 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tacgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg ccgcatcccg gtaatgccgc ctgcatgggt
      781 gatcgtcgtg cgaatatagc caatcagctg ctcttcgatt aactgcttgc agtcccagat
      841 actgttaaac acttcaccga ccgcaatcgg cgtcacggta tgctggcgaa tcagacgaaa
      901 acattcctgg ttctcggcag gcgtcggatc ttccatccag aacaggcggt aatcctcaat
      961 gctcttccca aagcgcgccg cttcgatggg ggtcaggcga tggtgcatgt catgcaacag
     1021 atgttcgtta aaaccgaact tattacgcac cgcttcgaat aacttcggcg taaaatcgag
     1081 atacttttct gaagaccaca actgctcttc tggccacagg cctttggttg ccggttcata
     1141 cgccagtcct ttgcccttcg ccaggccata agtggtttgc atacccggta caccgcactg
     1201 cacacgaatc gccttaaagc ccatctgctg gtgctttgca tagtcttcaa ggacttcatc
     1261 aatcgtacgc ccggtggtgt ggcaataaac catcacgcct tcacgagaag caccaccaag
     1321 cagttgatac aatggcatat ttgccgcttt agctttgata tcccacaacg ccatatcaat
     1381 ggcagaaatg gctgacatcg tgacggggcc acgtcgccag taagcacctt tataaaagaa
     1441 ttgccagata tcttcgatac ggctggcatc gcggccaatt agttgtggac aaagatgatc
     1501 tttcagataa gaagcgaccg acaattcgcg accatttaac gtggcatctc ctaatccaca
     1561 aagtccgctt tcagtggtta ttttaagcgt aacgaaatta cgccctgggc aagtaacaaa
     1621 aacatctgca gcaataattt tcattattgc aatctcctgt gttgtaaata gacgtttcaa
     1681 acctaatttt acgcctacta ccatacaaga gagaaaaagc gattattcaa ccgtgatcac
     1741 attaaaaaca aaaatttcca ccgaggagaa aaaataccct caaaaaccac ctgaataatt
     1801 aaccattatt gttaaaacaa cacgttaaat aatcacataa caaactccat taatcattga
     1861 aaatgagcac agaaatagaa attaattata ttcaattaat taaaatgtgt attgcttgat
     1921 gtatttaaaa atattatttt catggtgaga gtggaggtaa agaagacaca ataataaaaa
     1981 ggaatgtgac aaataatggt tctggttggg cgttaaattt aaggttacca gaa
//
LOCUS       DADLCQ010000275         2031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000275 DADLCQ010000000
VERSION     DADLCQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.278"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>2031
                     /gene="sat"
                     /locus_tag="JQG95_004205"
     CDS             <1..>2031
                     /gene="sat"
                     /locus_tag="JQG95_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY5890539.1"
                     /translation="GMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIA
                     SVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEK
                     ITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGKESIV
                     NWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGNGKVI
                     LNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEAVLAI
                     NNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTMQGHA
                     TEHAIFRSTASHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQPDWKT
                     GVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNNGFDF
                     KQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQ
                     NSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTG
                     NIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAI
                     WHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          745 a          358 c          401 g          527 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca g
//
LOCUS       DADLCQ010000276         1989 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000276 DADLCQ010000000
VERSION     DADLCQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.279"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..200)
                     /locus_tag="JQG95_004206"
     CDS             complement(<1..200)
                     /locus_tag="JQG95_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY5890540.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATL"
     gene            complement(204..947)
                     /locus_tag="JQG95_004207"
     CDS             complement(204..947)
                     /locus_tag="JQG95_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY5890541.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(838..>1989)
                     /locus_tag="JQG95_004208"
     CDS             complement(838..>1989)
                     /locus_tag="JQG95_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY5890542.1"
                     /translation="DVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLD
                     RWMVDPALTRRIKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKM
                     TENEWGMSVVERIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEAL
                     LKNIDLIRQFQSNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLF
                     GQSPKGFSTGDADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFN
                     PLWQMSDVDRSTVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIE
                     NAEDEAPPGIGEPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
BASE COUNT          427 a          579 c          506 g          477 t
ORIGIN      
        1 agcgtggccg gatggaatac ctgctcagga gaacgcgtga cgactatttc aacgaactta
       61 tcgggtttca gttttggcag gtcatcagca ccatagagct gcttacctgt tcgtcctatc
      121 ggcacgtctt tgcacagcaa cgagccgtca gccaggcggt atcgggtttc cccgagcctg
      181 gtctcaaaaa agtatttcat gggttatgct gcccttttca ttgcgaatgg aacgcgaaca
      241 aacacgatct ctttatagca gcggcagttt ggcagctcgc ccgcgtggcc ggtcatgccg
      301 tccaaagttg gaggtgaatc ccagcggacg aatttacctt tcatgtgatc gtgggaatct
      361 cggacatcgc catcatcggc tgtacgccaa atataaccat ccgagccaat agctatcgct
      421 cgggccatat ccagcgcgcc cgttgctctt cccagttccg ttcgggcaat cagatcggcc
      481 ctagacttag caacatcgcc ggtagatgca atctctttag caaactgctc cgcccgacca
      541 ccggtgacaa cagcctctat cgctttgttc tggatgtcgt acaccctgtc tgcggcctcg
      601 agaggcagag atttgatgta cttgacctgt tcggcgatga tggattgcat cacctggccc
      661 acaggagcgc tttccacaag attgcggagc tcgcgactga tgttcttgct gtgttgccgc
      721 caaactttct cgttctgccg ggttaggtcc gcagtaaagt tttccgcgac ctttgtcgcc
      781 cagggggtga tgatttcact gtaacgctcc agcgccgcaa taatttccgt gatactgtca
      841 tttgaaccat cgtagcgacc atttacgatg tctccgaccg ccctcgctat cctgcgtagg
      901 ctggttcgat accggatttc cgcctgacgg ctcctgcggt tcgtcatcag gttcgccgat
      961 gccgggcggc gcttcatctt cggcattctc gatgtcctcg ttggtaatgg atgcccctat
     1021 gccggttacg tcagaatttt ctcgcaaatc agtcatagcg gctttcagtg tcatcagacc
     1081 atcacccagc gccgtactga ttgcgttggt ggtgtttaac gccaccgttg agcgatcgac
     1141 atcagacatt tgccagagcg ggttaaactc aaacgtgaaa tcgtccggca gcggcttgcc
     1201 aagttccgaa cgatgcatga tgtccagtat ccgccgcacc ggaagacgta aacgcctctc
     1261 ctgcaacgaa ctgatgcgat cgtaatagtt ggcaaggtct gcatcaccgg tagaaaatcc
     1321 tttcggggac tgtccgaaca accgcaccag tgggatacca acagcgccac taatctgttc
     1381 tgcaaactgc gaaaggatgt catccagacc actgaagctg tactgatggg tttcaaactt
     1441 atcccgcgag tccatgagcg tcatgccttc attactctgg aactgtcgaa tcaggtcgat
     1501 attcttcagc aacgcttcat acgcaggacc accaagtgcg ataagctcgc gtagcttctc
     1561 cacgctgtag gtacgcaaat gcgccttgta gaccagctgc gccgcgccga cagtagcgct
     1621 gtcgaacgcg gtaagacgat cccagatacg ctctacaacc gacattcccc attcgttctc
     1681 ggtcatcttc tgctgaaatg gcaacgtgac gccatcaaag cgaatcaggc gactgtgatg
     1741 aatgcgccag gcaggaattc ccgttgctgt ggtcaccaca tcgtaaaact caggtttacc
     1801 caggtccggc cccatatctt taatgcggcg ggtcagtgcc gggtcgacca tccagcggtc
     1861 gagcgggaga atccccttaa acttgccctt accgatggtt tcgggtcgca gcggggtcat
     1921 tggtgcctgc ccctcaatca tgatgaaacc caccgcgccg ccgtagaggc gcgaccattt
     1981 cagcacgtc
//
LOCUS       DADLCQ010000277         1968 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000277 DADLCQ010000000
VERSION     DADLCQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.280"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..549
                     /locus_tag="JQG95_004209"
     CDS             <1..549
                     /locus_tag="JQG95_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY5890543.1"
                     /translation="VRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSI
                     LKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVK
                     TQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGS
                     QLLLLIRFLMHARNSGVLKWKA"
     gene            550..915
                     /locus_tag="JQG95_004210"
     CDS             550..915
                     /locus_tag="JQG95_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY5890544.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTYAGLLIDDEQFDEINIVC
                     GQLVPGGRLGIKITELGCA"
     gene            912..1595
                     /locus_tag="JQG95_004211"
     CDS             912..1595
                     /locus_tag="JQG95_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY5890545.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1794..1869
                     /locus_tag="JQG95_004212"
     tRNA            1794..1869
                     /locus_tag="JQG95_004212"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1827..1829,aa:Ile,seq:cat)
     gene            1871..1947
                     /locus_tag="JQG95_004213"
     tRNA            1871..1947
                     /locus_tag="JQG95_004213"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1905..1907,aa:Lys,seq:ttt)
BASE COUNT          539 a          431 c          546 g          452 t
ORIGIN      
        1 gtccgcaatc aggtggcgga cgcgctaccg gaagcggtat tacgtcgttc gctggggttg
       61 cgtgcggaaa aaatccgctc aatgtaccgt gaaagcgaca tcgtgccggg agagcagacc
      121 gccaccagca tactgaagca gcgcacaaaa aatcttgcgc cgctgcctca cgcccaccag
      181 caacagaatc caccacagga agagacggtg gtcagcattg ccgttgatcc ggaatctccg
      241 gctcagtatc tccagcgcca gaaaccacaa cgggaagaga tgcctgtata cacgcgctgg
      301 gtaaaaacgc agaaatgcat gacgtgtggc aatcaggcag atgatccgca ccacatcatt
      361 ggtcatggac tgggagggat gggaacaaag gctgacgatt tgtttgttat tccgctgtgt
      421 cgtaaatgcc ataacgaact acacgccggg gtaaaagatt ttgaagaaaa acacggtagt
      481 cagttgttgt tgctgattcg ttttttaatg cacgcgagaa attcgggtgt tctgaagtgg
      541 aaagcataaa tgaccgaacg catagaattt gttttgcctt acccgccaac ggtgaacacc
      601 tactggcgac gtcgtggcag cacatatttt gtatcaaaag ccggtgagcg ttatcgccgt
      661 gatgtggcgc taattgttcg ccagcagcga ctgaaattaa acctgtccgg aaggctggcg
      721 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaag
      781 gcaccgctgg atgcgctgac gtatgctgga ctacttatag acgacgagca gtttgatgaa
      841 atcaatattg tgtgcggtca gctcgttcct ggtgggcggc tggggataaa aatcacagaa
      901 ctggggtgcg catgaataac cagtatttac agtttgtgcg tgagcagctc attatcgcca
      961 ccgctgattt gagtggggca acaaaaggtc agcttgaagc ctggcaagag aatgccatgt
     1021 tcgatacagg gcgttacagg cgtaaaaaaa tccggtaccg cgatgaagtg actggaaaaa
     1081 tgataacgcg ggataatcca ccaatcccgg gaaagcaatc gctggcgaag gggacgtcaa
     1141 ttcctctggt cagtccggtt gagttttcga catcatcgtg gcggcgggct gttctgtctc
     1201 ttgaagagca ctataaagcc tggttgttgt ggtgttacag cgggagtatt tgttgggaat
     1261 atcagatcgc gataacacag tgggcgtgga atgaatttaa tgctcaatcc ggtaccagaa
     1321 aaattgcagg gaaaacgcag gaacgcctga aaaaattaat ctggctggcg gcgcaggcag
     1381 taaaagcaga actttttggt ggggaaggtt atgaatacca ggagctggca ttactggcgg
     1441 gagtgacaac taaaaactgg tccaaaacat ttactcgtca ctgggttgca atgaaacaca
     1501 tttttcaccg actggatagt gaggctttat tgtttgtaat gagaacacgt tcaaaaaaaa
     1561 gacggcattt tcaaagcaaa gtgttgcaaa agtagattga aaggcatata ttcatgcaaa
     1621 tctgatactt tgccgatttt gtacgtgatg gcaaaagcaa acaaaacccg cccacaagcg
     1681 ggttttttgt gccacttatc tcggatagac atggtgaatg cgctggtgga ggagataagg
     1741 gtgatttttg aatgcttgca acattgattt cgtaacgtta ttatcctgcg cccggccctt
     1801 tagctcagtg gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa
     1861 gggccaccaa ccgccactag ctcatcagga aagagcgtca accctttaag ttgagtgtgc
     1921 gaggttcgag tccccggtgg cggtccagtg ccgacttggc tcagtagg
//
LOCUS       DADLCQ010000278         1964 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000278 DADLCQ010000000
VERSION     DADLCQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.281"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="JQG95_004214"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="JQG95_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY5890546.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          333 c          311 g          698 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga catcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgt
//
LOCUS       DADLCQ010000279         1954 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000279 DADLCQ010000000
VERSION     DADLCQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.282"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            323..576
                     /locus_tag="JQG95_004215"
                     /pseudo
     CDS             323..576
                     /locus_tag="JQG95_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            733..>1954
                     /locus_tag="JQG95_004216"
     CDS             733..>1954
                     /locus_tag="JQG95_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890547.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSRPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGVSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAA"
BASE COUNT          514 a          386 c          445 g          609 t
ORIGIN      
        1 atctgcgacc cagacctgat taggggggtt ggggagcaat ggaacagtaa acgccgttaa
       61 gaagtcaatt taaatagcca aaaattaact agccaattta cattgcaggt tgtaattttt
      121 aaccaccttt tttacgatag ccaaattacc tatacccatc ccggccctgc caagccatac
      181 ccgctatcgg cgatgaaaat gcttttgtgc gcggtcggag tggtcccgac gagggtttta
      241 cccgaagtcg gggcgtatct ccgcgttagc gggccgtgag ggccgcttac gagcgtgtat
      301 gagagacgta cagtggtttt gtccgctcac accagtacat acaggtacgg aaataaaacc
      361 agtcgaaatg ccggtaacga cgattactcc tgtctctgat gccggtggac tacaagattt
      421 tatttactgg cgtcctgatg cggacaggac tggtatggaa cctgtttatg tgatacttaa
      481 tgatcctctg gattccgggc gatttacgcg taaacaacta aataaaaata tcttaaacat
      541 gcaagtaatt ttggtattct cgatacaaga ataaatagta gtaactacta tagttgcaaa
      601 taatgttaaa aacatttcta tgatcatctt tacatttttc atgattgaca attgtttttg
      661 gttaaaaaaa gacaactaat gagtgtgata atgcaatact ttgagtgatt atataatgat
      721 aagtaaacag gtttgcgaca attggttatc agtattacag aaggattaaa tatgtcatta
      781 ttcacagaac ctaaggaaat tgaaagatta ccctctgaag agattgaacg attatatccc
      841 gtcctgcgat acagggtctt tatcagcatt ttcctcggat atatggggta ttattttgtc
      901 cgaaacacca ccagtgtgtt atccggtgtg ctgcacatgt ctgcaaccga gatcggcatt
      961 atctcctgtg ccggtttttt atcctatggt atatcaaagt ttgtttccgg acttatttct
     1021 gacagaagta attcaaaagt atttttgtct cttggtttat tcctgtccgg actggtaaat
     1081 tttctgatag gatatattcc cgggatcatc acatccgtta cgttattttc aacaatgtat
     1141 ttacttaacg gctggatcca aggtatggga tatcctcctg gtgccaaaac actggtgttc
     1201 tggtatgaac atcgggaaag gatcacctgg gccacactgt ggaatttatc gcacaatgtc
     1261 gggggagctc tggcccccgt tctgattggt ttcagttttg gttttttcgg cgacagtgct
     1321 ctggatcatg cccgggccgc atttatcttc ccaggagtac tgtgtatggc aatgagtgtg
     1381 ctgatttact ttattcaggt ggacagacct gtatcagtgg ggcttcctcc cattgaagaa
     1441 tggaaaggta acgtcgtttc acgcccggct aaaggccgtg aacaaggtcc gagactctcc
     1501 attcctgata tcatcagaaa acatatcatc cgaaataata aactcattta ctgctgtatc
     1561 tatggttcgt ttgtttatat tctgagatac ggtattgttt cctgggctcc taagttttta
     1621 agtgacagtc tggatgtcgg tggtaaggat atgggaaaac tggcatcaat ggggggggtt
     1681 tctgtttttg aaatcggtgg tgtggctggt atgcttcttg ccggatacct gtctgtcaga
     1741 ctgttcagaa actcaaaacc attaacaaat actttatttc tggcacttac cattatttta
     1801 ttaatcgcgt actggtacgt gccatcaggc aatgaatatc tctggctaaa ctatacaatc
     1861 cttattctgt tggggctggc agtgtatggt ccggtgatgt ttattggtct ttattcaatg
     1921 gagcttgttc ctaaagaagc tgccggagcc gcat
//
LOCUS       DADLCQ010000280         1936 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000280 DADLCQ010000000
VERSION     DADLCQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.283"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..546)
                     /locus_tag="JQG95_004217"
     CDS             complement(<1..546)
                     /locus_tag="JQG95_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890548.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1201..>1936)
                     /locus_tag="JQG95_004218"
     CDS             complement(1201..>1936)
                     /locus_tag="JQG95_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890549.1"
                     /translation="NGAFNPAKDAPSTTVIKGASVTVTPVLNPETNKTGILCSAATHV
                     SDLDMMGGWTEGALIQTVCDMQVQYCRQMFAAVVDVLKDTSDLQIIEAAPLSGKPSDQ
                     AEDLLDTLALNLPVELGNTLSDYAVLVPEKLEAILDRAAQRAGHEGISELLGCTVCGY
                     AGDDTGVYLLPKRFASISFRSTKDAKTVDVKVTRNSNTAGYDLELISVVDVLATGSVK
                     VKAGEFDVEEDASFPLVHVIRFTAAE"
BASE COUNT          545 a          441 c          417 g          533 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgagtttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggaacctag gtatcgaggg tatcgaagat aatcagccgc taaccatttg tttccttaaa
      841 ggatgatgaa tccttgtggt tacagttatg gttacacatt aatttatctc tggtcaagtc
      901 ccgaagtcct aactttaaga cagtggcccc aatcgtccag acttgaaagg ggcctatggg
      961 ggaagtcgct gacctttatc tattaaatac ctacagagat ttttgtgaga ttttttaatc
     1021 caaggtcata acctactgcc cccacaggcc gcaaagtgaa gacctgtaag caccagagca
     1081 atagcgagta tctatcactc cctgtagagt atctggtaga ccaagaggac acagaggacg
     1141 accatataca actcctgtaa gctcatggat gtactcctta tgttcactca gggtttatag
     1201 ttactctgcg gcagtaaaac gaataacatg gaccaacggg aaagaggcgt cttcctcaac
     1261 gtcaaactca cctgctttca ccttaacgga accagtagcc aacacatcga ccacactgat
     1321 taactcaagg tcatacccag cagtgttgct atttcgggtc actttgacat ctaccgtctt
     1381 agcgtcttta gttgacctga aagagataga agcgaaccgc ttaggcagga gatagacacc
     1441 tgtatcatcg cccgcatagc cgcatacagt gcagcccaat agctcactaa taccttcatg
     1501 acctgcgcgt tgagcggctc ggtccaagat tgcctcaagt ttctcaggga ccaatacagc
     1561 gtaatcgctc agtgtatttc ccaactcgac cggaagattc aacgctaatg tatccagaag
     1621 atcttcagct tgatcagacg gtttaccact tagtggggct gcctcaatga tttgaaggtc
     1681 tgacgtatct ttcaggacat cgaccacagc ggcgaacatc tggcggcagt attgaacctg
     1741 catatcgcat acagtctgga ttaatgcccc ctcggtccaa ccgcccatca tatcgaggtc
     1801 agacacatga gtagcagcag aacacaggat gcccgtctta ttggtctcag ggttcaacac
     1861 tggggtgaca gttacgcttg cccctttgat gactgtggta gacggagcat ccttagctgg
     1921 attaaacgcg ccatta
//
LOCUS       DADLCQ010000281         1936 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000281 DADLCQ010000000
VERSION     DADLCQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.284"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQG95_004219"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JQG95_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY5890550.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1936
                     /locus_tag="JQG95_004220"
     CDS             1494..>1936
                     /locus_tag="JQG95_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY5890551.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          552 a          337 c          466 g          581 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatga
//
LOCUS       DADLCQ010000282         1913 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000282 DADLCQ010000000
VERSION     DADLCQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.285"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(53..1702)
                     /gene="treF"
                     /locus_tag="JQG95_004221"
     CDS             complement(53..1702)
                     /gene="treF"
                     /locus_tag="JQG95_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY5890552.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALTVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          451 a          506 c          492 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accgtcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DADLCQ010000283         1913 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000283 DADLCQ010000000
VERSION     DADLCQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.286"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..836)
                     /locus_tag="JQG95_004222"
                     /pseudo
     CDS             complement(1..836)
                     /locus_tag="JQG95_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1313..1549
                     /locus_tag="JQG95_004223"
     CDS             1313..1549
                     /locus_tag="JQG95_004223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890553.1"
                     /translation="MSALKETASMLKLFNEAKTQAEVDRAVSEINSKLSSIQLEYLSL
                     IDELTISKSNEAALKAKLTEIEKFKFQSEDCEPR"
BASE COUNT          514 a          450 c          442 g          507 t
ORIGIN      
        1 gctgctctac ggtcatattt ttaattgcgc tccggtttac aagagcccac ccctgttttt
       61 ccagataaaa ctggaaagta tccagggtac agaccagtgc accgtcagga acagtttcgg
      121 tgaatctgat attgccgtgt tcgtcgaagt gcacaaccag agtgcgaccg ttacccggaa
      181 tcatcttgtc agcggacggg gtattattct ggtgcagttc tgcctccatg cggtcgaaca
      241 tcagcgatgt aggcctcttt gaatgcagcg gcttttttcc cagtgaagcc catcaccagg
      301 aaaacgaagc cgtttttggt gatttggtac attggacgtt tttcgccttt ggtgtcggtg
      361 taggtgacgt cctcaaaatt gagggcgtta aattcaggtg agcattcagt atttgcgatg
      421 gcacgtaaca cattgtcgtg tcgtttgtgg aagaactctg caaccgcaac agacgtagtg
      481 acagcgcgac cgttttcgat ggttacgtca gggtgagaaa gggcagggat agtagccatg
      541 atggcatcct cgagtgataa gttaattaac tcaccaccga ggttttccac gaccataagg
      601 gtggtgagac gtacaggggt ggaaataccg gtcactcgag aacccggcca gtcttgcgac
      661 tgccctgcac atcccaccat aatttgaatg gagctgtgca ttacgcataa aaaaaccgct
      721 tcagcgcggc tatgcgctcg agtaacgttc gggtttccac gcccggcccc cgttttatga
      781 ggtgcagatg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      841 tcttaccttt catcaccgaa gtgaactttg ttgatgcggt gcctggtgcc tccaggtgac
      901 gttaaccagt taacaattaa cgccggatac agggaaaccc ataaccaccc gatacgtggg
      961 aactaatcgc tttttaactg ttccgcgtgc gctcagccgc attcaccgca tcacaaaatt
     1021 cactttaaaa agggcggaca tcagccagca atgaaactga tgccgccaaa ggtaccaatc
     1081 aacatggagt gttgtggcgt ggttgtcact taagcgtatg gtcaacctga caacccggtg
     1141 tcctcaacgg ggaaggaata actccgccat acttaccgcc gcgtcatttc gcgttgtgtg
     1201 cctgctttta actacgtcag gcgaggtggt tcctgttatt ccccaacaac aagaaatttg
     1261 tataatctga ataccccaac aataagaaga gtgagcaagt gatgtattag cgatgtcagc
     1321 tctcaaagag actgcatcaa tgttaaaatt attcaatgaa gcaaaaactc aggctgaggt
     1381 tgatcgcgct gtgtctgaaa taaactctaa gttgtcaagc attcagcttg aatatttgtc
     1441 tctcattgat gagctgacaa tctctaaaag caatgaggct gcgctcaaag caaaactcac
     1501 ggagatagaa aagtttaagt tccaaagtga agactgcgaa cctcgctaat tgattactgg
     1561 aacgattgtg tatgccagac agtgcaatac tagctctggc aagattacca tgtatttttg
     1621 tccagaatgt tttgcgaaga gagcaatatc tatattgcaa cctttcccta tcgaggaagg
     1681 cgataaatat acgaaaactc aatgcccctc ctgtaaaaat gagtatctca tggactacaa
     1741 tttcaataat gatttttgag tcaaggtatt cagattttta aagaacatgc cggatactca
     1801 cccgtgtccg gcgcacgcac tccacctcac ccgtggagaa ctccttaatt accaaccttg
     1861 gcttcgttgg ttagccatta acgctggtat gtaatcattc tggcaatgct taa
//
LOCUS       DADLCQ010000284         1888 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000284 DADLCQ010000000
VERSION     DADLCQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.287"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="JQG95_004224"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="JQG95_004224"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY5890554.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>1888
                     /locus_tag="JQG95_004225"
     CDS             1647..>1888
                     /locus_tag="JQG95_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5890555.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          516 a          387 c          480 g          505 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaa
//
LOCUS       DADLCQ010000285         1886 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000285 DADLCQ010000000
VERSION     DADLCQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.288"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..224)
                     /locus_tag="JQG95_004226"
     CDS             complement(<1..224)
                     /locus_tag="JQG95_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY5890556.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADA"
     gene            complement(221..754)
                     /locus_tag="JQG95_004227"
     CDS             complement(221..754)
                     /locus_tag="JQG95_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY5890557.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(820..1449)
                     /locus_tag="JQG95_004228"
     CDS             complement(820..1449)
                     /locus_tag="JQG95_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890558.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNGAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1453..1668)
                     /locus_tag="JQG95_004229"
     CDS             complement(1453..1668)
                     /locus_tag="JQG95_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY5890559.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          489 a          500 c          408 g          489 t
ORIGIN      
        1 gcatcagcta actccttcgt gtatttagca tcgagtgcat cagcatcacg ctggcgctgc
       61 tgcatgtcag taatggttgc gttcgccagc ttcagttctc cggcgttttt gtcgcgctgc
      121 tctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
      181 atgcagataa ccagagcgga gataatcgcg gtgactctgc tcattgatct atcccccaac
      241 aggctaatgc gctttcctgg tcacgacgaa taacctgtcc atagcagtta tttgaacgta
      301 tgcggcaatc gcgcccaaca tcttttatcc accagcgaat cgcctcgcat gcgcccttac
      361 gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg gggccaatgt
      421 tataggggca aaatgacgct atacccgctt tctggggttc ggtcagaggt actttaatat
      481 tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg tcacattttt
      541 ccttcgacag cttcattccc ggtatgacag gcttaccatc caccattgtg gcaccacgac
      601 agatggtcca tatgccagaa ccatcacggt atgccgttgt gtgattaccc tctttttcgt
      661 tcagaaactg gtcaagtatc tggggagcag acgcgcctgc ggcaatcagc gccagaacag
      721 cagccgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat ttatcggcaa
      781 caaataacga gccaacttat atacgccttt taagataagt cagccccggc tggatccagt
      841 catcaggctc tttcttaaag ggggagtatt gaaatcacga agaagagcct cccgcaccgc
      901 agcatccatg tctgcaccac tggcaagtgc ttcaatctct gccgccacct gcaaataccc
      961 catgcaacgg ccaatgcgtt tcataactct ggtgttttcg tctttcaggc atctgatagc
     1021 gagattaata attttcaggc agtgatgatg cgatgcccgg acaggttttc cgttctcata
     1081 aacgacgaac tcaccctctt cacaggaaac cctgaaaggc cgatggggtt cctggtcttt
     1141 atccggtgca gacgcaataa aatcactttt atgctcttcc tctccggcac cgttaaccgt
     1201 aatgacgtac tcaggccgca ggtaagccgc ctctttttct gacaatgcgg ccggtaaaaa
     1261 cttttcctgc aaaaacttca taaaagtctc gtgagcgact ttgtggcagt aatcgtaaat
     1321 cgccgcataa tgctcatcgc ggcgtttgtt ttcatcttca gaactcacca acgccgacaa
     1381 ttttttatta agctcagtga tttcattttc caggtgattg aatcgctgat tcatttcttc
     1441 atgcttcatt atttactctc cccgtgcagc cttacgcttg tcttctctta ttttgaaata
     1501 cagattcgtc agataagtca gaagccccag aagcagactt cccagcacac caatcgcagc
     1561 ccactgtgat ggcctgacct gatccaacca ctgcaaaaac cagtagccgg cactgccggc
     1621 ggaggtgccg taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc
     1681 gcaaataacg gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaaa
     1741 cgcaaaagcg ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccaccc
     1801 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcacgcccga aggttagtat
     1861 ttctgcacag caattttgca aaaaaa
//
LOCUS       DADLCQ010000286         1879 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000286 DADLCQ010000000
VERSION     DADLCQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.289"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            334..557
                     /locus_tag="JQG95_004230"
                     /pseudo
     CDS             334..557
                     /locus_tag="JQG95_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            666..932
                     /gene="ospE1"
                     /locus_tag="JQG95_004231"
     CDS             666..932
                     /gene="ospE1"
                     /locus_tag="JQG95_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY5890560.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1323..>1879
                     /locus_tag="JQG95_004232"
     CDS             1323..>1879
                     /locus_tag="JQG95_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890561.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNQ
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          634 a          392 c          314 g          539 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aatgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacataaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaattttttg ccaaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttccaca aaaacaagaa aatatcatcc
      721 ttgaagtatc aaatcctgta ttattatctt ctactgttac tacagatgga tatactgttt
      781 tcaataaaaa agcagctata tacgaattac aaatacctgc tgccagccgg acgaaaacgt
      841 tgaaattcac tgctacagaa atgcaatggt tgacaaagat aaatgaagcc ggaattgatg
      901 aaaaacaaag ccaacggtat tctgatttct gaagttaatt tcagactcgt ttaagctcca
      961 ttttcaaaaa aatatatcag ggtttgttac taataacttc tgaaaacaga aaaaaaaata
     1021 gagcagtaat tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt
     1081 tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat
     1141 gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc agactccttc
     1201 ccatagactg cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta
     1261 aaattaataa gctatattct atttaactag aattcttatc gttgataaga attctagtta
     1321 taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca
     1381 atgtatactc gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat
     1441 tttatttaaa aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata
     1501 tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg
     1561 agctagattt aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa
     1621 aaaaccaatt aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat
     1681 ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg
     1741 ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata
     1801 ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg
     1861 gaaacagatt agacagcat
//
LOCUS       DADLCQ010000287         1870 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000287 DADLCQ010000000
VERSION     DADLCQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.290"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..1000)
                     /locus_tag="JQG95_004233"
     CDS             complement(<1..1000)
                     /locus_tag="JQG95_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HAY5890562.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWDEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDESAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNS"
     gene            complement(1003..1557)
                     /locus_tag="JQG95_004234"
     CDS             complement(1003..1557)
                     /locus_tag="JQG95_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY5890563.1"
                     /translation="MKQLTPKQELFCREYLKDLNATQAAIRAGYSEKTARATGSENLS
                     KPDIADRIAELKAERNEEVGIDAAYVLRRLTEIDQMDVLDILLANGELKQIKDWPKVW
                     RTTLSGMDVVEMASADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(1559..>1870)
                     /locus_tag="JQG95_004235"
     CDS             complement(1559..>1870)
                     /locus_tag="JQG95_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015996068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890564.1"
                     /translation="KDLVVRHKCDNPSCVNPDHLIIGTVADNNRDRSRRGRNSYPDRS
                     GEKNGMAKLKECDVICIRREYVRGSVGNGLRSLAKRYGVSTTMIADIVNHRNWSNVSE
                     V"
BASE COUNT          432 a          517 c          413 g          508 t
ORIGIN      
        1 aggaattttt gtccctgcct tcgtcggcga cgtccatcgc gcccagtcgc ttgcctgttg
       61 gctggatgcc cagcttgata tgcgcgtcga cagcagcctg tacccattcg gatggaatca
      121 ggacgccttc cgctgatgca ctgtagttca ggtcaagttc ctgtgccacc accaccggat
      181 tgtcgatttt ctcgcattcc ctgcgatacc actcttcatc cttgcgagga tcatcccgcc
      241 agtggaatgt gaataccggt atcttcccgc catgacgctt ctgagcgaac gggttcgcca
      301 tgccgttaac tgaactcagg tcaatacggc aacgcgtcgt ttgtgacaac gccgcatcaa
      361 tcagcagagg acgctgaagg aatgcagact catcaaccag ataaagcgtg gtacggtcac
      421 cacggccaat attatcgcca gcctcacctt tgataacggc accagtttca ggaaactcaa
      481 cgcgcatgta tggtgcgtgc ttcttttcgt cccacgaacc tcgaaactct acaggtagtg
      541 tttccacgaa cttgcgcgcc ttccagaaca atgctttcgg gtcaccggtg ctgtcgacgt
      601 attcctcttt acgggagccg aaaccgataa ccatttcttt gttgaagaga caaagcgagc
      661 atgccagccc gatcgcggtc caactgagcc ccatttcacg ggatttttcg gtaataccat
      721 tctcccgatt accccagcgt tccataatcc agtggatcca ctcctcctgc ttagggaaga
      781 gtaaaaacgg aatggtcacc ggcaggccat aatcaatatt acgcgggtcc gttgtcatgc
      841 cccagtcgat gatgaactga gccggattgg ttcggtaaaa ctgttttagt gcaggcaata
      901 tttcaggatt ctggcgaatg cgctgtaggc gttccatccg ccattcaaaa accatctggt
      961 aatcaggatg tttaaaatcg aaggggaatg gtaacggcat acttagccca tcatttttct
     1021 atacgcctct gcagcctgct ccggcgttaa gttggtaatt tctgttctga ctggtcctcc
     1081 gtcagcacca gtcacttcat ttttgacatt gtctttaaac gcctgaacag aaacatgacg
     1141 cccgagcaac tcaaggtttt taaccttatc aggccatttg attttcttca ggagagcagc
     1201 actatctgct gatgccatct cgacgacatc cattcctgat agcgttgtac gccacacctt
     1261 aggccagtct ttaatctgct tcagttcgcc attggcaagc agaatgtcaa gcacgtccat
     1321 ctgatcgatt tcagtcagcc gtcgcaatac ataggcggcg tctataccta cctcttcatt
     1381 gcgttcagcc tttagctcag caatgcgatc tgctatgtca ggttttgaca ggttctcgct
     1441 accagtggcg cgagcagttt tctcgctgta acccgccctg atagctgcct gagtggcgtt
     1501 taaatctttt aaatattccc tgcagaacag ttcctgctta ggtgtgagct gtttcaaatc
     1561 acacctccga aacattgctc caatttctat gattaacgat gtcagcaatc atggtagtgg
     1621 atacgccata tctctttgct aaagacctaa gcccattacc tacactcccg cgcacatact
     1681 cgcggcgaat gcagatgaca tcgcactctt ttaatttcgc cattccgttc ttttcacctg
     1741 aacggtcagg gtatgagttt ctgcctcgcc ttgacctgtc acgattattg tcagcgacag
     1801 tacctataat cagatggtca ggattcacgc atgatggatt atcgcattta tgcctaacaa
     1861 caaggtcctt
//
LOCUS       DADLCQ010000288         1838 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000288 DADLCQ010000000
VERSION     DADLCQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.291"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..180)
                     /locus_tag="JQG95_004236"
     CDS             complement(<1..180)
                     /locus_tag="JQG95_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY5890565.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(321..875)
                     /locus_tag="JQG95_004237"
     CDS             complement(321..875)
                     /locus_tag="JQG95_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY5890566.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(872..1162)
                     /locus_tag="JQG95_004238"
     CDS             complement(872..1162)
                     /locus_tag="JQG95_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY5890567.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1162..1761)
                     /locus_tag="JQG95_004239"
     CDS             complement(1162..1761)
                     /locus_tag="JQG95_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY5890568.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          448 a          498 c          421 g          471 t
ORIGIN      
        1 gttatgggtg atcggggaga gagtttcaac agagatttga gtggtcataa cgataactcc
       61 gtacatttgg aaattatcgc caccgacgac gccaatcgaa ctggtggcga actgtgcagg
      121 gttggcgtaa ccgggtacgg aaaccggcga gcctttcggc tcccccacac agcccgccat
      181 aaatcgcgaa tgtgactgtg caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc
      241 tccgtaaaca tccgggacgc caatcccgac gccagatttt gctggcgcgt gaggaatata
      301 gccccggata acagattgag tcaacaaacg gtttttagat ccccggaaga gaatgcatca
      361 cgcatcggta gatagagcat atattcagca agatttagcc atacatctac ccgactgcag
      421 cacgtccgga aacaccactc agggtgtgcg tcattcagca attcagccat tttacgctta
      481 gtcatccccc gcccttcata ccgttgccgg aggacactaa tcaatccagg atgctctgcc
      541 agcacctcac ttatgactct atcaatacat aacgcctctg catcagtaca atgcgccagc
      601 caggtctttt gcttgccatt gatcatctct cgcaaaaacg cttccagctc aggtttctct
      661 attcccgctt ttttcatcct gcgcagggct tcattgacgg ctgttttcgt caattttttg
      721 gatgccagca actggttaaa catatttccc gtcttaccgc cgccaatata cgaccagcgc
      781 ccccacatgc gcagttttcc ctgaatccag acactttcca gcgtggtgag acgaaggtgt
      841 tccccgcttt tgcctgtatt tgttgggtaa atcataaata accttccttt ctccagattt
      901 cttgtgtgcg aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc
      961 tggtttttac ccgcccgtcg attaaatcgt ggcatgagct acaggcaatc gctgcctgca
     1021 tatcgtgtgg ttttgtcgct gttccgcacg tccccgccag cctgtaatgc gccagcacag
     1081 aagtttcggg attgtgattg cagtagccag gaattctgac ggtgcacatc tgcccccgcg
     1141 ccgctttacg taaatccacc attacgcaaa ctccagtaac tgcgcggcca cattttcgac
     1201 ttcctccgga gaggaaaatt tacggaacag gatccagttc cacagcacat tcagtacaga
     1261 tttataaacc tgctgaaact cggtttcgtc catgttcgca aatgcgatag attttgccct
     1321 gcgcccacga ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac
     1381 ccactcgcgg aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc
     1441 aactgtatta aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac
     1501 tgattcgcac aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc
     1561 cccaccgacc ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg
     1621 gaatgcgtag ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg
     1681 atgcaaaaaa tcgcaactct ccggcgtcgc cgggagaagt aatccggaag aggtttgttt
     1741 gaccagttgt atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc
     1801 cgttatgtag tataccagaa ttaatgccaa tactaaca
//
LOCUS       DADLCQ010000289         1821 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000289 DADLCQ010000000
VERSION     DADLCQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.292"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..185)
                     /locus_tag="JQG95_004240"
     CDS             complement(<1..185)
                     /locus_tag="JQG95_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890569.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQG"
     gene            371..1213
                     /locus_tag="JQG95_004241"
     CDS             371..1213
                     /locus_tag="JQG95_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY5890570.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1216..>1821
                     /gene="capU"
                     /locus_tag="JQG95_004242"
     CDS             1216..>1821
                     /gene="capU"
                     /locus_tag="JQG95_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY5890571.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
BASE COUNT          528 a          338 c          397 g          558 t
ORIGIN      
        1 ccctgccgct catcacgtaa ttgtttcacc catcgcgtca ttgtggaaag gccgacatcc
       61 atagcgctgg ctgcatctgc cacggtgtat ttctggtcaa cgaccagttg agcggattcg
      121 cgtttaaact ctgcgctgaa atttcttttt tcattatggc acctgtgttg ttctgaggtg
      181 agcatatcac ctctgttcag gtagctaaat ttagtgtgcc acctcaaggt tcttagtgag
      241 ccgtttttaa aagatatgat aatatattat tgtttataaa ttgttgatgt ttgatttagt
      301 agtatataaa gttcaccacc tttattaggc tttcctttta tctctactta ctgggtgcca
      361 gaaactttaa attttaaacg gagggggcat tttgtttaaa gcaaatcatg ttccggtatt
      421 aatgtatcat catgtcagcc attgcccagg actggttaca ctttctcccg agacattccg
      481 gaaacaaatg aaatggttgg ctgaaaacaa ctggaaaaca ctgtcatcag atgaactgga
      541 gtttttttat cggggcggaa aacttccccg taaaagcgtt atgttgacat ttgatgatgg
      601 ataccttgac aactggtttc aggtatatcc tttattaaac gagtttaatc tcaaggcaca
      661 tattttttta attaccagtt ttattggaaa tggtccggtt cgtcactctc ctggaaagga
      721 atattctcat cgcgattgcg aacatcaaat agcaaccggg aatgctgata atgttatgct
      781 tcgctggtct gaagtgaacg aaatgttgca atctggtctg gtcgaatttc atgttcatac
      841 acatactcat acacgttggg ataaaaagtt cacttcacga gaagaacagt gtaaacatct
      901 tcggcaggac ttattgagcg ggagagaata tttaaaaaaa atgacaggaa aatgcagtaa
      961 acatttgtgt tggccggaag gttattataa caaggattat attcagatcg ctgaagaact
     1021 tggattccat tatttatata caacagaaag aagaatgaat gctcctgcta aaggaactac
     1081 acggatagga agaataagca ccaaggaacg ggaaaattgc gtatggctga aaagacgact
     1141 tttttattat acaacaccgt ttttttcatc gttgctggcc cttcataagg ggccaagatt
     1201 accggatgac taataatgaa tatactattt acggaatcat caccgaatat aggtggtcag
     1261 gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa tggggcattc agttctgctt
     1321 gtctgcaggg agaacagcaa aattgctttt gaagccagta agtttggaat tgatatcaca
     1381 ttcgcgttat ttcgaaacag tcttcacatt cctactgtat ggagattact cggaatagtt
     1441 catagttttc agcccgatgc aatcgtttgt cacagtggac atgatagtaa tattgttggt
     1501 ttggtacggt tttttacctg gaaacatcca ttcagaatta tcaggcaaaa gacatatttg
     1561 acacgaaaaa caaaattttt ttcaataaat catttttgcg atgaggtgat tgttcccgga
     1621 acaaatatga agacacatct ggagcaggaa ggatgtcgaa ctcgggttac tgttgtgcct
     1681 cctggctttg atttccagga attatacgtt gattcccgaa acagtttgcc tccgagtgtt
     1741 ctttcctggc tggcgtcccg aagaggatgc cccgtaattg ctcaggtagg aatgttgcgc
     1801 ccggaaaaag ggcacgaatt t
//
LOCUS       DADLCQ010000290         1816 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000290 DADLCQ010000000
VERSION     DADLCQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.293"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..325)
                     /locus_tag="JQG95_004243"
                     /pseudo
     CDS             complement(1..325)
                     /locus_tag="JQG95_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(325..1245)
                     /locus_tag="JQG95_004244"
     CDS             complement(325..1245)
                     /locus_tag="JQG95_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5890572.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1383..>1816
                     /locus_tag="JQG95_004245"
     CDS             1383..>1816
                     /locus_tag="JQG95_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5890573.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          452 a          435 c          422 g          507 t
ORIGIN      
        1 tgcgttcagc atcaacatat ggcagagtta ctgcataatc gataacacgg ctaatatcct
       61 ctacgcgctg tgtcggatct tctatccaac gaggctaccc accagattct ccctggaaac
      121 tggcgtcata agcgagaacg acatagcctt tttcagccaa tgccttgcca tagatattgc
      181 cagacgtctg ctccttacaa ctgccaaaag gatggacgct gataatcgcc ggatactgtt
      241 ttgactcatc aaaatttggt ggaaactgaa tattggcagc aatatgccag gccatatcac
      301 tattgcggat ttgaatatgt ttcattacag agtttcctca aagaacggag ctaatactga
      361 aattgcttca gccacatagg ctttgccgtc ataaagatcc atatggtttg cgccttcaac
      421 aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac aggtcgtcac tcatccattt
      481 gctccctgct tggctgcccg ccacaatctg catcggctga gtcaggtaca cttccgccat
      541 atggtaagca tcataggtaa taatctggtt aaggctgcgc aaagtagcgt aacccggtgc
      601 tgttggatac tgcgcacgag gtgtgtggta atactcccag gcctgacgca gttcttcgtt
      661 cggcgcatcg gcctccttca ttggagccag tggcataatg gcgtattctt cgctgctaat
      721 atcgctggtt cgggcgtttg agccagcttc aatgtgtggc agagcatcaa tagatttcac
      781 attgttttcc caaccattgc ggaacatcga accaatattg accgcactaa cggtaccgat
      841 ggccttgatg cggcgatcct gaattgcagc attggctgta tatcctgcac cggcacaaat
      901 tcccatcgca ccaattcggg tattgtcgac ataagaaagc gttgtcaggt aatcaatcac
      961 ggcactgacg tcttcagtac gaatgtatgg gttttctaac tgacgcggct cgccgccact
     1021 ttcaccttga taagatgcgt cataagcaat agtgacaaat cccttttccg ccagcttttc
     1081 ggcataggtt ccggccgttt gctctttaac gcccccacct gggtgagata ccacaattgc
     1141 cggatactga cgggtttcat caaattttgg agggaaatag atcactgcag acaaagagat
     1201 ggttggatta ttgctgttag tgaagctgac tttattattc atcattcagt tcctcatgga
     1261 gttgtcggtt cgttttgacg gttggtgata tcactataga tattgatcat taagttgatt
     1321 agaccctaaa tcatgattag acctatcatt taaatgatta atagattgta tggagatact
     1381 gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt cgtcgttgca gaggagcgta
     1441 gcttcactcg cgcagcagcc cgcctgagca tggtgcagtc agctttaagc cagatagtgc
     1501 gccgtataga agaacgattg ggattgcggc ttctgacgcg aaccacgcgc agcgttgtac
     1561 ccactgaagc gggcgagcat cttttgtctg ttcttggccc tatattgcat gacatagatt
     1621 ctgccctgac atccctgagc gatctgcaga accgcccatc cgggacaata cgtattacta
     1681 ctgtagaaca tgcagcaaaa acgatattgt taccagccat gcgcacattc ctgaaatcgc
     1741 atcctgaaat tgatattcag cttaccattg attatggttt gaccgatgtc gtttctgaac
     1801 gttttgatgc aggcgt
//
LOCUS       DADLCQ010000291         1810 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000291 DADLCQ010000000
VERSION     DADLCQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.294"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..161
                     /locus_tag="JQG95_004246"
     CDS             <1..161
                     /locus_tag="JQG95_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5890574.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JQG95_004247"
     CDS             655..1233
                     /locus_tag="JQG95_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890575.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JQG95_004248"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JQG95_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..1810
                     /locus_tag="JQG95_004249"
                     /pseudo
     CDS             1584..1810
                     /locus_tag="JQG95_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          587 a          322 c          424 g          477 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc
//
LOCUS       DADLCQ010000292         1808 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000292 DADLCQ010000000
VERSION     DADLCQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.295"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            173..1198
                     /locus_tag="JQG95_004250"
     CDS             173..1198
                     /locus_tag="JQG95_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY5890576.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1808
                     /locus_tag="JQG95_004251"
                     /pseudo
     CDS             1266..1808
                     /locus_tag="JQG95_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          420 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctagct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtggg
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgtc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgtt
//
LOCUS       DADLCQ010000293         1802 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000293 DADLCQ010000000
VERSION     DADLCQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.296"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..376)
                     /locus_tag="JQG95_004252"
     CDS             complement(<1..376)
                     /locus_tag="JQG95_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY5890577.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVE"
     gene            complement(414..623)
                     /gene="hha"
                     /locus_tag="JQG95_004253"
     CDS             complement(414..623)
                     /gene="hha"
                     /locus_tag="JQG95_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY5890578.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(669..1142)
                     /locus_tag="JQG95_004254"
     CDS             complement(669..1142)
                     /locus_tag="JQG95_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY5890579.1"
                     /translation="MENNMRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSR
                     KAVRIRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDG
                     MNVNQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1375..1587)
                     /locus_tag="JQG95_004255"
     CDS             complement(1375..1587)
                     /locus_tag="JQG95_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY5890580.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          445 a          486 c          356 g          515 t
ORIGIN      
        1 gctcaacgat aaccacatca acatgccact gagacaccat cctgaataac tgccaccact
       61 ggcgtgatcg tggccggata ttcccgttca gctggacgat atccgcgacc cgtacctgcg
      121 gacacagata ccaccgcttc atatccacaa ttctgaacag tcctttcata aaactgactt
      181 ccctttctgt cagcagcgtc ttctgacgtt gccagtattc accgccggac agtattcgct
      241 ctgcatcacc ggtccgtata cctgccgcat gcaactgcat cagcaaagga gaatgctgat
      301 ttacacttct gaggataatc cggataagta acgctgcaat aatcagaaca atgagaataa
      361 aaataatcag cttcattgtt tatctgctcc tggtaattaa ttatgctgaa ttattacctt
      421 acatatttcc atatctcttt tggtatcctg tcatataact ttccggtgat taattccgct
      481 aaccggtggt ctgctgctga attaaatgat tcacgttcac tggtcgataa actgtctctg
      541 tttttatgaa tgattttttc cagcgtattg actgatgaac atcgccgtaa ctgatacagc
      601 cattcctgtt ttgtttttgc catttttatt cccgctgaga acagatgaat gataagattg
      661 ccatcgcttt atttacggtg tctccatatc caggggggaa ccggatcagc atctgaccag
      721 agcccacgtt tttgttgtct ggcttcgttc tgaaggacag gcagcacggg atctgtgttg
      781 tactgctcat atacccaggc agccccggcc cggaccataa actgattaac attcattccg
      841 tctggtgcat aaacctgccc gagaatacga ccataacgat ctcgctgaaa atatgtgacg
      901 gtaaccgttt ttcctgcaac cagtgatttc atcatgtccg ttgaccaccg gccataatcc
      961 tgtttctttt ccggtgcatc aatatttatc agcctgatcc gcactgcctt ccgtgagtcc
     1021 atgacctcga tggtatcacc gtccagtaca cgaacaaccc gtccatgaat atctgcacac
     1081 aataccggcc atgaaaagat aaataatacc agtggaatat atttcctcat gttattctcc
     1141 atatcagata aatggcacct gtatttcagg taatcctgaa tatcgcagca cacactgaat
     1201 aacaccttat tttgtgaaat taatcacaaa atataaaatc acgacttaat acggaaaaaa
     1261 ctctgtgcta ctcagaactg tccggtcata cttcccgacc ggatattaaa aaaagcaaat
     1321 ctcatgcatt attcagcctt ctgaatccct ggagtaatga aggctgcttt ttcgttacac
     1381 ccttccccgg cagcgccggt ggcagtgttc agcccttttt cgggcaaact gctgccagtc
     1441 tgtccgggga gcagactgcg cagcccgtag ccatgcctca gcggctcccg gccagtcccc
     1501 cctgacttcc agccggacag cccggcgtcc gacctgccgg tagcgccata ccccggagtt
     1561 ttgcgaatca cgccctgttc cgttcatttt ccttccccct ttaatccagg ccggtgaatg
     1621 tgttataaat ttttataacc ttcttctgcc gggtatggaa gaaggtttca gagaagcctt
     1681 atttttccgc ctcctttagt gtgaaggagg cttttttatt gttcatcaag cacggcctga
     1741 agttctgcct ttatccggtt ctggcggcgg attttatcca gacgctctgc cgcatacact
     1801 tc
//
LOCUS       DADLCQ010000294         1764 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000294 DADLCQ010000000
VERSION     DADLCQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.297"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            240..509
                     /locus_tag="JQG95_004256"
                     /pseudo
     CDS             240..509
                     /locus_tag="JQG95_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="JQG95_004257"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="JQG95_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY5890581.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          517 a          370 c          413 g          464 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gagagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 gcaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac ggggtgtggc cgga
//
LOCUS       DADLCQ010000295         1759 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000295 DADLCQ010000000
VERSION     DADLCQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.298"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..263)
                     /locus_tag="JQG95_004258"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="JQG95_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /locus_tag="JQG95_004259"
     CDS             complement(378..1016)
                     /locus_tag="JQG95_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890582.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="JQG95_004260"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="JQG95_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          501 a          435 c          318 g          505 t
ORIGIN      
        1 agcaagcact gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttacttttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagta ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatctt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcgt gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg actaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaat
//
LOCUS       DADLCQ010000296         1745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000296 DADLCQ010000000
VERSION     DADLCQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.299"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            344..1300
                     /locus_tag="JQG95_004261"
     CDS             344..1300
                     /locus_tag="JQG95_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5890583.1"
                     /translation="MVWVGKPTVIVNWLTSPGGTRHRINKVHFGDERKEKMLNVAIEN
                     QNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLCVKHSHIQIMVGRAGQPQGWPVS
                     VVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATILTLSHPDATIENGRAVTTSVAV
                     AEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTDAKGEKRPMYQITKNGFVFLVMG
                     FTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVVHFDKFGNVEFTET
                     VPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLLSLK"
     gene            1317..1598
                     /locus_tag="JQG95_004262"
     CDS             1317..1598
                     /locus_tag="JQG95_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890584.1"
                     /translation="MNPMLDYCFTSAPQQTITHTGIKNLLWIIKKNLQQKGDPDLIPC
                     AYGLCDRQVSPDKAYTAELIYMHGTMMRKKKRKYCSEICAEKDQMAHEL"
BASE COUNT          497 a          399 c          410 g          439 t
ORIGIN      
        1 cattatatag ttctactctg gcgagatacg ttggcatatt catttcctta ctggttgtgt
       61 gagaacttca gtaaagatac caccaaagcc cggaagtggt gaaataaaac cgggcacaac
      121 acgaaggcgc atttccggta tccataaaga gtcggtcttg tctgtgaaat ttaaatggtg
      181 ggagtgcgct tccggttgta aataacgaca ttgctgtgtg aagtaccagt tggcggcatc
      241 ggtttaattg ctggctgatg tccgcccttt ttaaagtgaa ttttgtgatg cggtgaatgc
      301 ggctatgcgc acgcggcaca gttaaaagtc atgttagtcc ttattggttt gggtgggaaa
      361 gccaactgta attgttaact ggttaacgtc acctggaggc accaggcacc gcatcaacaa
      421 agttcacttc ggtgatgaaa ggaaagagaa aatgttgaat gtagctattg aaaaccagaa
      481 cgggtggaat tatagtgcac ctgcacctca taaaacgggt gccgggattg ctaccccgac
      541 gatgactact gcgcataacc gcgctcaggc ggttttttta tgcgtcaagc acagccacat
      601 tcagattatg gtggggcgtg cagggcagcc gcaaggctgg ccggtttcgg tagtcaccgg
      661 ttgtagcaac cctgtgcgtc tcaccaccca tgagattgct acctctggtg gtgagtcatt
      721 taaactgact atcgaggctg ccatcatggc tactatcctt accctttctc accctgacgc
      781 aaccatcgaa aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa
      841 aatgcacaaa aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg
      901 gcttaatttt aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca
      961 aatcaccaaa aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc
     1021 attcaaggaa gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa
     1081 caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga
     1141 caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg tctgtaccct
     1201 ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc ggggcgcaat
     1261 taaaaatatg actgtggaac aattactttc tttaaaataa aaatggagct acggatatga
     1321 accctatgct tgattattgt tttacctctg cacctcagca gaccataaca cacaccggaa
     1381 tcaaaaattt gttatggata atcaaaaaaa acctccagca gaaaggtgat cccgatttaa
     1441 ttccctgtgc ttatggcctg tgtgacagac aagtaagccc tgataaggct tacactgctg
     1501 aacttattta catgcacgga accatgatga ggaaaaagaa acgcaaatat tgtagtgaaa
     1561 tctgcgccga aaaagaccag atggcacacg aactttaatt aactgactat tcgaaactga
     1621 atttatgcca gcaatggcag ggattcgctc aaccttaatt aaggagaaaa acatgaacac
     1681 caattatgaa gccactgttg caactactga taacattgtt cacgaggttt acctggaagg
     1741 aaagc
//
LOCUS       DADLCQ010000297         1717 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000297 DADLCQ010000000
VERSION     DADLCQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.300"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(110..577)
                     /locus_tag="JQG95_004263"
     CDS             complement(110..577)
                     /locus_tag="JQG95_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY5890585.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKTNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="JQG95_004264"
     CDS             complement(643..882)
                     /locus_tag="JQG95_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY5890586.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="JQG95_004265"
     CDS             complement(1017..1622)
                     /locus_tag="JQG95_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY5890587.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          379 c          303 g          486 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tggttttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata ttttttggat
      961 gtggctataa ttatccattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DADLCQ010000298         1703 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000298 DADLCQ010000000
VERSION     DADLCQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.301"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(821..1060)
                     /locus_tag="JQG95_004266"
     CDS             complement(821..1060)
                     /locus_tag="JQG95_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY5890588.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1612)
                     /locus_tag="JQG95_004267"
                     /pseudo
     CDS             complement(1255..1612)
                     /locus_tag="JQG95_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          474 c          412 g          438 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctactcgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacctgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccaaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgt atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggc gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aag
//
LOCUS       DADLCQ010000299         1682 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000299 DADLCQ010000000
VERSION     DADLCQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.302"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>1682
                     /locus_tag="JQG95_004268"
     CDS             <1..>1682
                     /locus_tag="JQG95_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY5890589.1"
                     /translation="QDGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDS
                     GQRTGTGSALMSMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGE
                     ESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPG
                     MVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMP
                     VADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRAEPATLPVADSPFTALK
                     LENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKL
                     ARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMV
                     DLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSI
                     LRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQ
                     FRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQ"
BASE COUNT          362 a          457 c          557 g          306 t
ORIGIN      
        1 tgcaggatgg catgaccttc actccgggca gtaccgttat cgttgaccag ggcgaaaaac
       61 tctccctgaa agagacgtta accctgctgg acggtgccgc acgtcataac gtacagatcc
      121 tgataaccga cagcgggcag cgaaccggta caggcagtgc gctgatgtcc atgaaggatg
      181 ccggggtgaa cacatatcgc tggcagggcg gagaacagcg accggccacc atcatcagtg
      241 aaccggaccg taatgtccgc tatgcccggc tggccggaga ttttgcggcc agcgtgaaag
      301 ccggagaaga gagcgtggca caggtcagcg gggtacggga acaggccata ctgacacagg
      361 ccattcgcag tgagctgaaa acacagggcg tgctcggaca cccggaggtg accatgaccg
      421 ccctgtcacc ggtctggctg gacagccgga gccgttatct gcgggatatg taccgaccgg
      481 ggatggtgat ggagcagtgg aacccggaga cacgcagtca tgaccgctat gttatcgacc
      541 gggtgacggc gcagagtcac agcctgaccc tgcgggatgc gcagggcgaa acgcaggtgg
      601 tgcgtatttc ctccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg
      661 cagacggcga gcgactgagg gtgacaggga aaattcccgg gctccgcgtc tccggcggtg
      721 accgcctgca ggtggcatcc gtcagtgaag atgtgatgac ggttgttgtg ccggggcggg
      781 ctgaaccggc caccctgcct gtggctgatt caccgttcac ggcactgaag ctggagaacg
      841 gctgggtgga aacgcccggg cattccgtca gtgacagtgc gacggtgttt gcctccgtca
      901 cacagatggc aatggacaac gccaccctga acggtctggc ccgcagcggt cgtgatgtcc
      961 ggctgtattc ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct
     1021 ttacggtggt ttctgagcag ataaaggcgc gggccggtga gacattgctg gaaaccgcta
     1081 tcagtctgca gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc
     1141 ctgtgctgga aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt
     1201 cgtttgctgc agaaggaacc ggttttactg aactgggagg ggaaatcaat gcgcagataa
     1261 aacgcggtga cttactgtat gtggatgtgg caaaaggcta tggcaccggc ctgctggttt
     1321 cccgtgcgtc gtatgaggca gaaaaaagta ttcttcgcca tattctcgaa ggtaaggagg
     1381 cggtcacgcc gctgatggag agagtacccg gcgaactcat ggagacactg acgtcgggac
     1441 agcgtgccgc cacccgtatg atactggaaa cgtccgaccg tttcacggtg gtacaggggt
     1501 atgccggtgt gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc
     1561 cggcgagtga gcgtccccgt gttgtggggc tggggcccac gcaccgtgcg gtcggggaga
     1621 tgcgcagcgc cggcgtggat gcacagacac tggcgtcctt tctgcatgac acgcagctgc
     1681 ag
//
LOCUS       DADLCQ010000300         1662 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000300 DADLCQ010000000
VERSION     DADLCQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.303"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..366
                     /gene="traX"
                     /locus_tag="JQG95_004269"
     CDS             <1..366
                     /gene="traX"
                     /locus_tag="JQG95_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY5890590.1"
                     /translation="TWCETRSGWRTAAAILLMALWGPLSGSSYGIAGLLMLAVSHRLY
                     RAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKSLPRFWPG
                     DFFPVFYACHLAVLGVLAL"
     gene            425..1285
                     /locus_tag="JQG95_004270"
     CDS             425..1285
                     /locus_tag="JQG95_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY5890591.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            1388..>1662
                     /gene="finO"
                     /locus_tag="JQG95_004271"
     CDS             1388..>1662
                     /gene="finO"
                     /locus_tag="JQG95_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY5890592.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDT"
BASE COUNT          436 a          344 c          470 g          412 t
ORIGIN      
        1 acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct gatggccctg
       61 tgggggcctt tgtccggctc cagttacggc attgccgggc tgctgatgct ggcagtcagc
      121 caccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg
      181 gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc cggtcttgta
      241 atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa aatcattacc ccgtttctgg
      301 cccggggatt ttttcccggt attctacgcc tgtcatctgg ctgtgctggg cgttctggcg
      361 ttgtgactgg tatgatactg acgctgattc tgattattaa taaacaacat aacacaggat
      421 atttatgaaa attacggatc acaaactctc tgaaggtatt gccctgacat ttcgtgttcc
      481 tgaaggaaat ataaaacatc ctttgattat cttatgccat gggttttgcg gtatcaggaa
      541 tgttctgctc ccttgctttg cgaatgcctt cacagaagca ggattcgcaa cgattacttt
      601 tgattatcga ggattcggtg agagtgatgg agaacgaggg cgtctggttc ctgcaatgca
      661 gaccgaagat attatttcag ttatcaattg ggcagaaaag caggaatgta ttgataatca
      721 gcgtattggg ttatggggaa catcgctggg aggggggcat gtcttcagtg cagctgcgca
      781 ggaccaacgc gtaaaatgta ttgtcagcca gctggctttt gctgacggtg atgttctggt
      841 taccggagag atgaatgaat cggagagagc gtcttttctg tccacgctga ataaaatggc
      901 agagaagaag aaaaacaccg ggaaagagat gtttgtgggg gttacccggg tactgagtga
      961 taatgaatca aaggtattct tcgagaaggt aaaaggtcag tatcctgaaa tggatataaa
     1021 aatacctttc cttacggtta tggagacgtt gcaatataaa ccggcagaga gcgcagccaa
     1081 agtacagtgc ccagttttga ttgttattgc cggccaggat agtgttaatc caccagaaca
     1141 gggaaaagcc ttatatgatg ctgttgcttc tggtactaag gaattatacg aggaagctga
     1201 cgcatgccat tatgatattt atgaaggcgc gttttttgaa cgtgtggcgg cagtacagac
     1261 gcaatggttt aaaaaacatc tttaattgtc ggtattaata tgtcagaggt cgttgaggtc
     1321 agcttaatat catagtgagt agtttcaacg acctggagga catattttca caaaggtcag
     1381 ggcaatcatg acagagcaga aacgaccggt actgacactg aaacggaaaa cggaagggga
     1441 aacaccgacc cggagccgga aaaccatcat caatgtcacc acgccaccaa aatggaaggt
     1501 gaaaaagcag aaactggcgg agaaggctgc ccgggaagca gagctgacag caaaaaaagc
     1561 gcaggccaga caggcgctgt ccatttatct gaacctgccc tcgctggatg aggccgtgaa
     1621 caccctgaaa ccctggtggc cgggattatt tgacggtgac ac
//
LOCUS       DADLCQ010000301         1645 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000301 DADLCQ010000000
VERSION     DADLCQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.304"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(156..1391)
                     /locus_tag="JQG95_004272"
     CDS             complement(156..1391)
                     /locus_tag="JQG95_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY5890593.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JQG95_004273"
     tRNA            complement(1538..1614)
                     /locus_tag="JQG95_004273"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          417 a          448 c          393 g          387 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caatt
//
LOCUS       DADLCQ010000302         1614 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000302 DADLCQ010000000
VERSION     DADLCQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.305"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..1614
                     /locus_tag="JQG95_004274"
                     /pseudo
     CDS             1..1614
                     /locus_tag="JQG95_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          361 a          425 c          532 g          296 t
ORIGIN      
        1 tgctggaagg aatggccttc acgccgggca gtaccgttat cgtcgaccag ggcgaaaaac
       61 tctccctgaa agagacgtta accctgctgg atggtgccgc acgtcataac gtacaggtcc
      121 tgataaccga cagcgggcag cgaaccggta caggcagtgc actgatggcc atgaaggatg
      181 ccggggtgaa cacatatcgc tggcaggggg gagaacagcg accggccacc atcatcagtg
      241 aaccggaccg taatgtcggc tggccggaga ttttgcggcc agcgtgaaag ccggagaaga
      301 gagcgtggca caggtcagcg gagtacggga acaggccata ctgacacagg ccattcgcag
      361 tgagctgaaa acacagggcg tgctcggaca cccggaggta accatgaccg ccctgtcacc
      421 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgcccgg gaatggtgat
      481 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gtgacagaac gtgtgacggc
      541 gcagagtcac agcctgaccc tgcggaatgc gcagggcgaa acgcaggtgg tgcgtatttc
      601 ttccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggtga
      661 gcgtctgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
      721 ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc
      781 caccctgcct gtgagcgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga
      841 aacgcccggg cattccgtca gcgacagtgc gacggttttt gcctccgtca cacagatggc
      901 aatggacaat gccaccctga atggtctggc ccgcagtggt cgtgatgtcc ggctgtattc
      961 ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt
     1021 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     1081 gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc ctgtgctgga
     1141 aagtaaaaaa ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     1201 agaaggaacc ggttttgctg acctgggagg ggaaatcaat gcgcagataa aacggggtga
     1261 tttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     1321 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc
     1381 gctgatggag agagtacctg gcgaactcat ggagaaactg acatcaggac agcgcgccgc
     1441 cacccgtatg atactggaaa cgtctgaccg tttcacggtg gtgcagggct atgccggtgt
     1501 gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga
     1561 acgtccccgt gtcgtggggc tggggcccac acaccgtgcg gtcggggaga tgcg
//
LOCUS       DADLCQ010000303         1601 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000303 DADLCQ010000000
VERSION     DADLCQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.306"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(333..479)
                     /locus_tag="JQG95_004275"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="JQG95_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="JQG95_004276"
     CDS             533..715
                     /locus_tag="JQG95_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY5890594.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="JQG95_004277"
     CDS             complement(816..1145)
                     /locus_tag="JQG95_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY5890595.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1601)
                     /locus_tag="JQG95_004278"
     CDS             complement(1317..>1601)
                     /locus_tag="JQG95_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890596.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          436 a          368 c          376 g          421 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt cagaaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat aacacgaaat g
//
LOCUS       DADLCQ010000304         1598 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000304 DADLCQ010000000
VERSION     DADLCQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.307"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            154..477
                     /locus_tag="JQG95_004279"
     CDS             154..477
                     /locus_tag="JQG95_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY5890597.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            474..890
                     /locus_tag="JQG95_004280"
     CDS             474..890
                     /locus_tag="JQG95_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY5890598.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            856..1380
                     /locus_tag="JQG95_004281"
     CDS             856..1380
                     /locus_tag="JQG95_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890599.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERRRHWTQELLSR
                     ELQRSLRPVKRKHK"
     gene            1377..>1598
                     /locus_tag="JQG95_004282"
     CDS             1377..>1598
                     /locus_tag="JQG95_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890600.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLS"
BASE COUNT          389 a          379 c          456 g          374 t
ORIGIN      
        1 gttcgtgggt ttttatacca ccaagcgaac cggcggtatg ctggtggatt ctcaggcgat
       61 taagctgatg aaaattgggg ctgcaacccg ccagaaagcc gctgcgtaat gcggtttttt
      121 gtgcccgcgt aatcgcgggc acaggaggaa aatatgctcc tgaaagaaga ggagattaaa
      181 tctcatctcc ggctcgatga tggtttgtac agtgacggcg attttctgaa actgctggca
      241 caggcggtac agaaaaaaac agagacatac ctgaacagga agttgtatgc accggaagag
      301 acgattccgg aagatgatcc tgacgggatg catctgactg atgacgttcg tctggcaatg
      361 ctgatgctgg tcagtcattt ttatgaaaat cgctcaacga ttaccgatgt ggagaaactg
      421 gaaacgccaa tgagtttcag atggcttgct ggcccttaca ggattgtccc gctatgaaaa
      481 tcaggcaaag tcagaccagt gccacttacc ttttaccgga tcccggagag ctgaatcgcc
      541 ggataaagat ccgccttcgt gtggatgaac ccaccgctga ttttggtacg gagccaacgt
      601 atccggagtc gtttgatgtc tgggcaaagg tggctcagcc aggcgctgcc gcttatcagg
      661 gctcagtgca gacagaaaat atcgttacgc attattttac gatccgtttc cggcacgaca
      721 tcacggcaga tcacgaagtg gtttattacg gccaagagta ccggatccgg cgaatacgtg
      781 acctgaacgg tcagcggcgt tttttattac tggaatgtga agaactgcgt acagcgcgac
      841 gacggggtga atgccatgaa tcagacagca tttttacacg tcgactttaa acaaccagag
      901 gagctggagt ttaatcgcgc ccgtctgcgt cgggcgtttg tgcaaatcgg gcgcgtttac
      961 atgcgtgatg ccagacgtct ggtgattaaa cgcggacgct ccggtccggg agagaacccg
     1021 ggatatcaga ccgggcgtct ggcccgctcc attggttatt acgttcccaa aaaaacgacg
     1081 cgtcgccccg gactgatggt gaaaatttcc cctgaccaga aaaacgggca ggggaatcgc
     1141 cgttttcctg aaggtgctcc ttattatccg gcgtttctgt attacggcgt tcgccattca
     1201 gcgtatggaa tggataaaaa ggataagcgg cagaagaagc accattcttc gactttccgg
     1261 ctggccccgc gtaataactt tatggctgac gttattgagc gtcggcgtca ctggacgcag
     1321 gaactgttgt cccgtgagct gcagcgttcg ttacgtccgg taaaaaggaa gcataaatga
     1381 aactgacaac gataattgcg gcactgcgtg agcgatgccc gcgttttgag gagcgtgttg
     1441 gtggcgcggc gcagtttaag gcgatccctg atgccggaaa acttcgcctg cctgctgcct
     1501 atgtggttcc ctctgacgat gcgccggggg aacaaaaatc acagaccgat tactggcagg
     1561 atttgactga aggcttctcc gtgattgtgg tgctcagc
//
LOCUS       DADLCQ010000305         1597 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000305 DADLCQ010000000
VERSION     DADLCQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.308"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..702)
                     /locus_tag="JQG95_004283"
                     /pseudo
     CDS             complement(1..702)
                     /locus_tag="JQG95_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(750..1322)
                     /locus_tag="JQG95_004284"
     CDS             complement(750..1322)
                     /locus_tag="JQG95_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY5890601.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGKIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          475 a          336 c          318 g          468 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aattttaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtatttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DADLCQ010000306         1564 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000306 DADLCQ010000000
VERSION     DADLCQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.309"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            281..1357
                     /locus_tag="JQG95_004285"
     CDS             281..1357
                     /locus_tag="JQG95_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HAY5890602.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTTRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLEVLEVTAPDRMERRKRGKSD
                     KIDAECAAHAAFSGIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQINSIPITA"
     gene            1377..1564
                     /locus_tag="JQG95_004286"
                     /pseudo
     CDS             1377..1564
                     /locus_tag="JQG95_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          404 a          372 c          407 g          381 t
ORIGIN      
        1 gtaataatgc cggtatcagt ttttatcatc actctgtttg ctgtttaacc agactggtgt
       61 gattactgat gcagtgaaga ccttcccgca tcctgactca cacagcgatc gaccctttgt
      121 gtcctgccct ggacctgtcg gttgccggaa gcgtcttcat gcgaggcgtc tcctcaccga
      181 tgcgcgtgac tcaagaaggg cctgacggct tgtctcgtta ctgtcctgtc cgggttatct
      241 gtctggagat tcaactctgt ttcctcacag gagctctgtt atggcaggta aagttacgga
      301 aaccgctgtt gtgggtggcg tggatacaca taaagatctg cacgttgccg ctgtcgtaga
      361 tcagaacaat aaagttctgg ggacccagtt tttctccaca acacggcaag gttaccggca
      421 gatgctggca tggatgactt cgtttggggc attaaagcga attggtgttg agtgtacagg
      481 cacctatgga tcaggtctgc ttcgctattt acagaatgcc gggttagaag ttcttgaggt
      541 gactgcgcca gatcggatgg agcgacgcaa acggggtaaa agtgacaaga ttgatgctga
      601 atgtgccgct cacgccgcat tctccggaat aagaaccgtc acacccaaaa cgcgcaatgg
      661 catgattgag tctctgcggg tattaaaaac ttgccgaaaa acagcaatat cagcccgcag
      721 agtcgctctc cagattatcc attccaatat tatctctgcc ccggatgaat tacgtgaaca
      781 actcagaaat atgacgcgca tgcagctcat caggactctg ggatcctggc ggcctgatgc
      841 cagtgaatac cgcaatgtta ccaacgttta tcgtatttca ttaaagtccc ttgcccgacg
      901 ctatctcgag ttacatgacg aaatcgctga tttggatgtc atgattgcgg caattgtcga
      961 tgagctggcg cctgaactga ttaaacgtaa tgctattgga tacgaaagcg cttcgcagtt
     1021 gctgatcacg gcaggagaca atccccaacg attaagatca gaatcaggtt ttgcggcact
     1081 gtgtggtgtc agccctgttc ccgtatcttc aggaaaaacg aatcgttatc gacttaaccg
     1141 gggtggagat cgtgctgcaa atagtgcact tcacatcatt gccatcggac gtttgcgaac
     1201 tgacgataaa acgaaggaat atgtcgccag acgagtagcg gaagggcata caaaaatgga
     1261 agcaatacgc tgcctgaagc gctatatctc acgcgaagtt tatacattac tgcgtaatca
     1321 aaacaggcag atcaacagca tcccgataac ggcttgactc ttagaagggc gtccatggca
     1381 gtcattatac gagcaggcag ttccggcagt tactgtggcg ataccagatc aggcagagta
     1441 tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc agaagtctga
     1501 agaacgaatg gataccggtg gtgggttacg taagcttcag cgaggcagct cacgccataa
     1561 cgga
//
LOCUS       DADLCQ010000307         1538 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000307 DADLCQ010000000
VERSION     DADLCQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.310"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(910..1386)
                     /locus_tag="JQG95_004287"
     CDS             complement(910..1386)
                     /locus_tag="JQG95_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890603.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          347 a          410 c          385 g          396 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gcgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gctcgatcat gtttttgccg tccgttcacc gttaaaacgc ctcagaaatc
      241 gggacaggat gtgggaatgt cggaccgcgc acgcgcactc agacattccc cacttggtca
      301 gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc aatgtaactt ccggttctct
      361 tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg acttgcggcg agcgggatca
      421 cctctcgcat gttgtcggtg atcccgttgt tgttctttcg gggatgagtc gtcagatagc
      481 ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg gtttaaatgc ccgtcagggc
      541 atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc cgatacccct
      601 aaccaggggc tttcaggtcg ccaggaaccg cgtcagctcc ctgcaaagcc tatttgcacg
      661 gcacaggcag ctattcggct ggttggtggt cgtagaccgt cggtgttaca gcgatggggg
      721 tctggggttc tccccagacg gtttccggtt gcgctcaagg cgcagccgga accgtggcgg
      781 cgcggtcgtt ctggggatcg gggagcaaaa acacgcaagg gattttgagg cggcaaaaga
      841 gcaatgcagg atagacaatc atggtcaaaa cgttaacacg tagcccctga ttgtccgcct
      901 gccgctggct caatttgaaa acatggccat gattcggcgc tgaatatcac cgtctttgtt
      961 gaccagtgtt ttagtgacaa acgctgcatg gtttttcgta gtgttgtatc ccttccagac
     1021 ttcatgtgga tcgagggcat acgcacaaac cttaccagca acggggattc taactaatgc
     1081 attttttgat tctaagtaat tgagccaccg aataattgtg cttctgctaa ctccaagttt
     1141 ttcagctagg aattgctgat ccgcaacgac agcgccacac gtaggatcga tatgctctgc
     1201 taaaaatgag tacagcttca gtgctgatat gccttggcta tccttcagca gctctcgaac
     1261 gcgttcccaa ccttttgggg atacctgtgt aaaccttgaa ttcttctttg cttcttcgag
     1321 atcttcggtg tttttctttg cctcatctga aagttgtttc agtcgttttt ctgaatcaat
     1381 aaacatgatt ttttccctct gaaaatccca tgcactaatg ttggttcaaa tctaagccat
     1441 catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tcctggatgc gacaacgttg
     1501 tgtcaccctg gatgcgacag gtaaacaaaa aacacact
//
LOCUS       DADLCQ010000308         1515 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000308 DADLCQ010000000
VERSION     DADLCQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.311"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..672)
                     /locus_tag="JQG95_004288"
     CDS             complement(<1..672)
                     /locus_tag="JQG95_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5890604.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLAGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVVELAAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWAISTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLT"
     gene            complement(684..1187)
                     /locus_tag="JQG95_004289"
     CDS             complement(684..1187)
                     /locus_tag="JQG95_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890605.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DADGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            complement(1192..>1515)
                     /locus_tag="JQG95_004290"
     CDS             complement(1192..>1515)
                     /locus_tag="JQG95_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890606.1"
                     /translation="TITGDSAWRAEVIVPGIDLSRKMKPTVFKREVLASADKTLVRQV
                     VGDADIRKLPKQSVDMAFNAVSEIAKGRNTRSTTGDAQRPNMGMTSIASLNKQNAEFW
                     SNRKG"
BASE COUNT          340 a          421 c          397 g          357 t
ORIGIN      
        1 cgtcaacagg gactggttac ccgcagatga aacgatgatg ctggacaaca gagcaaactg
       61 ctctggcggg atcagcaaat cacgcggaac cacagagtaa ccggaagcgg cccacgcatc
      121 agacagcacc tggttaatgc ttgcgcggat ttcgtccggt gttgagattg cccacgtttt
      181 ggcagcgttg ttgacaggca cgccgtccag ggtaacaagg cctttcaggt ttaatgcgga
      241 atcgccaaca tacacctgct cgtcgttgtc catctgccat ttgagctgca tcccgtcgta
      301 cttctgggtg tcaatcgggc gacctacctg ctgagctgct gccagctcta caacggtcca
      361 gcccagttcc atgccccaga gattcagcgg gttaccggat ttgccgatat ccacgtttac
      421 gccagcaata gcggttgagt ctttgcctac ccagtttttg ccattcggat ttgcaccagt
      481 acccgcagcg gcgaagctgg tattcgtcca gctggaaatg tcatctgcga tagagacatc
      541 ttcacgcaac tgaatatcgc gggtccaggt gtaccccgcc agtggcaggt tcagagtctg
      601 gtcgagtcgc tccagctctc cgatgagaaa ggcaccagag ctgtcaacgg ttgcctgatc
      661 aaaagtaatc attcgtctgt tccttaaatc ttccaggaaa tttctgcatt gccgttagca
      721 tcaccggcac ctgtgaattc agcgttggtc agcaccacat ttttgccact gactgacgtg
      781 gacatgaatc cacccagcgg cactttgatg gattcatcag tggagacgac aacgtatacc
      841 gggtcgcctt ttttgatggt gctggcatca aaatcagaac cgagattaac ggtcacgtag
      901 ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg aaccatgtcc
      961 ggctgcgaag tggtcggata agggcgcacg tagatcccct tcaccttgtc tgcggtatcg
     1021 ccatctgcca gcggcacgaa aaaaccgtca gcatcgtatt taccagccag cccataggca
     1081 gcaaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg cgggcgagag
     1141 atagccccgg caatgccaac aggcatccgg tacagatatg cagtcattgg attatccttt
     1201 gcggttagac cagaattcag cgttttgttt gttcagggaa gcgatgctgg tcatgcccat
     1261 gtttggacgt tgtgcatcgc ccgtggtgct gcgggtgttt cgccctttgg caatctcaga
     1321 cacagcgtta aacgccatat cgaccgattg cttaggcaat ttgcggatat ccgcatcacc
     1381 gacaacctgg cgaaccagcg ttttgtcagc agaagccaga acctcgcgtt tgaacacggt
     1441 cggtttcatc ttacggctca gatcgatacc cggaacgata acttcggcac gccaggctga
     1501 gtcaccagta atcgt
//
LOCUS       DADLCQ010000309         1504 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000309 DADLCQ010000000
VERSION     DADLCQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.312"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(108..620)
                     /locus_tag="JQG95_004291"
     CDS             complement(108..620)
                     /locus_tag="JQG95_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY5890607.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            815..>1504
                     /locus_tag="JQG95_004292"
     CDS             815..>1504
                     /locus_tag="JQG95_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890608.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGID"
BASE COUNT          452 a          325 c          377 g          350 t
ORIGIN      
        1 ccaatgcctt ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt
       61 cagaggaatg ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa
      121 ggcaacacga agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga
      181 ctcactgtat acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg
      241 taacgcagca cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac
      301 tgaaggcgaa cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg
      361 atatgcccaa ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc
      421 atgaccgtga ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg
      481 gccatcataa ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac
      541 gctattataa aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa
      601 gcatttatcc actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt
      661 gatacgactt aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc
      721 tgccacggag cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag
      781 gcagcgataa cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat
      841 cgaatcggca taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt
      901 tacagaaggg gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac
      961 gcaggtttgc aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat
     1021 tactggcctt acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc
     1081 gatacgcgga atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc
     1141 attaaaacac ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc
     1201 taaagcgttg acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc
     1261 agagaatatt ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa
     1321 gatgctgatt gaaaatatca gcgccgccga gaaagcaatg atgcgcaata cagttcgtat
     1381 tgagtccatc gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg
     1441 caaggccgcc actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat
     1501 tgat
//
LOCUS       DADLCQ010000310         1435 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000310 DADLCQ010000000
VERSION     DADLCQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.313"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..371
                     /locus_tag="JQG95_004293"
     CDS             <1..371
                     /locus_tag="JQG95_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890609.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="JQG95_004294"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="JQG95_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5890610.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..>1435
                     /locus_tag="JQG95_004295"
     CDS             738..>1435
                     /locus_tag="JQG95_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890611.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVVKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          340 a          399 c          397 g          299 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggc ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcgctggtt gtgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttacctg
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgc
//
LOCUS       DADLCQ010000311         1405 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000311 DADLCQ010000000
VERSION     DADLCQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.314"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..168
                     /locus_tag="JQG95_004296"
     CDS             <1..168
                     /locus_tag="JQG95_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY5890612.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            153..791
                     /locus_tag="JQG95_004297"
     CDS             153..791
                     /locus_tag="JQG95_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY5890613.1"
                     /translation="MGDSVTPETLGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..>1405
                     /locus_tag="JQG95_004298"
     CDS             1019..>1405
                     /locus_tag="JQG95_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890614.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          394 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgatttct ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ctctgtgaca ccggagacac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat gcaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa gatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagt
//
LOCUS       DADLCQ010000312         1380 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000312 DADLCQ010000000
VERSION     DADLCQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.315"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..253
                     /locus_tag="JQG95_004299"
                     /pseudo
     CDS             1..253
                     /locus_tag="JQG95_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1380
                     /locus_tag="JQG95_004300"
     CDS             746..>1380
                     /locus_tag="JQG95_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5890615.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTN
                     FVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQEL
                     CGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
BASE COUNT          338 a          320 c          421 g          301 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gctaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tccgctccgg catccagccg ttgcaccgga agtgttcgtt
      961 tgcgaattac cgggcgcaga acgacggtca gcgacatgca ctgagtcagg cgaaatccat
     1021 agctgacgaa ctgatgacag gctgtacgaa ttttgtgttc agcggtaagc ccggcaccgg
     1081 aaaaaatcat cttgcagccg cgattggcaa ccggctgatg gcgaaggggc gtagcgtgat
     1141 tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag agctacgaca acggcaaatc
     1201 cggtgaaaaa tttttacagg agctttgtgg tgttgacctg ctggtcctgg atgaaattgg
     1261 catgcagcgg gatacgaaaa acgagcaggt ggtactgaac cagattgttg atcgccggac
     1321 ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac catgcagcga tgaatacact
//
LOCUS       DADLCQ010000313         1355 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000313 DADLCQ010000000
VERSION     DADLCQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.316"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(2..484)
                     /locus_tag="JQG95_004301"
     CDS             complement(2..484)
                     /locus_tag="JQG95_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5890616.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(475..777)
                     /locus_tag="JQG95_004302"
     CDS             complement(475..777)
                     /locus_tag="JQG95_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY5890617.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1049..1321
                     /locus_tag="JQG95_004303"
     CDS             1049..1321
                     /locus_tag="JQG95_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890618.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          397 a          342 c          288 g          328 t
ORIGIN      
        1 ctcagacttt ataaaacaag gtattaggtg ccacaactga ctgaacaaaa ccaaaacgct
       61 cataaaatgc tctggcgtca tcatcaattg catgtaccat aacagcctta atgccaacct
      121 gatcagccag attgacaacc cggtgaatgg catcacttag tagccattta ccgaaaccat
      181 gcccctgggt acaaacatca acagccagac gccctaacag aacaacaggg acaggattgg
      241 gcatgttatg ccgcaaactg cgaccaagct cggcatgagt gacggaacca gtagctaatg
      301 agtagtatcc cacaatacgt aaagtatcct ccaagcaaat aacgaacgtg cgagaagcat
      361 tgagcatctg gtttttcata gcccgaccac gtaaccagtt acttagcacc tcatttccac
      421 agtcaaatgg ctgaagtata tgctcatccg tcaacaatgc tggcgcggtg acatttattt
      481 ccattcaggc tttaccgcca tcagacgttc acgcccttct gcattctgca caggagcttc
      541 cagttgcgta ataaacgaat cataacgttc gttatccagc acaaataaac gttgatccag
      601 gataatgttt ttagcttcct ggtacgccag ttccatgatg aaatcggtac ggttcttatt
      661 gaccaagctc gcagcataat caataacggc tcgttcctca tcagttgccc ggatgttaat
      721 ctgatgttct cgcggtgttt tacggacgct tgcagctgta gacataaaaa cctccattgg
      781 taacaatttg ctatgcaatt aatgttatcg caaatggtaa cagaacgcta tacagaatca
      841 cttacgggaa aagcgatccc ctgataaaaa caatggcaga gacctgaaca ttgtgacgat
      901 gatctgtgtc ggcgcacaaa tcatgtctga ttacggccgt ttaatgtgat tttgataatg
      961 aaatgcctga accacggcag cctggcggct gcaaatcggg gtgtggcaga atatgagctt
     1021 tcagagtaac catccagaat gagttcccat gtcacacaac ctggagcacc agaaagtcca
     1081 caccagaatg gtgaaagagg ttctgaaagc cgtcgccaga gccaataatc acccttataa
     1141 gtccgtattt gcagacttta tcaccggtca tccctcctgc acggtctgtt tctgggaaac
     1201 cttccacaaa atgtacccgg attcaccgta cgaatacgtc acgttctgcc atacctgccg
     1261 tcgttttgat ttatacgaaa cagaagcgga gatgaaggcg gatgacccga agtggtggtg
     1321 attttcagcc ccgaccgctg aaacgcctgt tcaca
//
LOCUS       DADLCQ010000314         1348 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000314 DADLCQ010000000
VERSION     DADLCQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.317"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1064
                     /locus_tag="JQG95_004304"
     CDS             <1..1064
                     /locus_tag="JQG95_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890619.1"
                     /translation="EERGAGAAISVLGYRLGMLVSGGLALWLADKWLGWQGMYWLMAA
                     LLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDFFGRNNAWLILLLIVLYKLGDAF
                     AMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGALYGGILMQRLSLFRALLIFGIL
                     QGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGTSAFVALLMTLCNKSFSATQFAL
                     LSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAAVPGLILLLVCRQTLEYTRVNDN
                     FISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDALDLTHFSFLPALLEVGVLVALS
                     GVMLGGLLDYLALRKTHLT"
BASE COUNT          254 a          320 c          349 g          425 t
ORIGIN      
        1 cagaagaacg tggtgcgggc gcggcaatca gcgtgctggg ttaccgttta gggatgctgg
       61 tttccggcgg cctagccctg tggctggcag ataaatggtt gggctggcag ggcatgtact
      121 ggttgatggc ggcgctgttg atcccctgta ttatcgcgac gttgcttgca ccagaaccaa
      181 ccgacaccat tcctgtgccc aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt
      241 tctttggtcg caataatgcc tggcttattt tgcttcttat cgtgctgtat aagctgggcg
      301 acgcattcgc catgagcctg acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg
      361 gtgaagtagg cgtggtaaac aaaacgcttg gcttactggc gaccattgtt ggcgcattgt
      421 acggtgggat tttgatgcag cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt
      481 tacaaggtgc gtctaacgct ggttactggc tgctgtcgat tactgataag catctctaca
      541 gcatgggcgc agccgtcttt ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg
      601 tcgcgctgtt aatgacgctg tgtaataagt cattttccgc tactcaattt gccctgctct
      661 cagcgctttc cgctgtaggg cgagtttatg tcggccccgt ggcgggttgg tttgttgaag
      721 cacacggctg gtcgacattc tatctattct ccgtcgccgc tgccgtaccg gggcttattt
      781 tgctgctggt ttgccgccag acgcttgaat atacacgagt aaatgacaac tttatctccc
      841 gtaccgaata tccggcaggt tatgcctttg ccatgtggac actggcggcg ggcgtcagcc
      901 tgttggccgt gtggttactt ctgttgacga tggacgcgct ggatttgacg cacttctctt
      961 tcctgcctgc tctgctggaa gtcggggttt tagtcgccct ttctggcgtc atgcttggtg
     1021 gtttgctgga ttatctggcg ctacgaaaaa cgcatctgac gtaatctgta aatattattt
     1081 agctgtagta atcactcgcc gtaattatta cggctaaata aacatcagca tcgcttatta
     1141 atatttgttc ttttgtgcgg cttagcgttt ggaaattatt gacgccattt ataaattcta
     1201 tttttcttac acgattcagc taatgagtct ttattttctc accacccagt tgtcacttta
     1261 atgataatta tttgttaaat aattgtttta tttcgcattg gttataccaa ttgcccgcca
     1321 agactccttc tgcactcccc ttttgtta
//
LOCUS       DADLCQ010000315         1345 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000315 DADLCQ010000000
VERSION     DADLCQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.318"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            443..1192
                     /locus_tag="JQG95_004305"
     CDS             443..1192
                     /locus_tag="JQG95_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY5890620.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          377 a          274 c          278 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctgggtgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcacacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcggaaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DADLCQ010000316         1326 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000316 DADLCQ010000000
VERSION     DADLCQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.319"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..1268
                     /locus_tag="JQG95_004306"
     CDS             <1..1268
                     /locus_tag="JQG95_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY5890621.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIVDGLSGGLIDGLKMLAVAVTAQKAANVAGGLLNLANAVTSFGDAVN
                     KAKAGKAGSSGGALNKVGRVVLYAELVNFALDNLNPEAAKNPNLHQSGIGTETNFNPE
                     YKGDSSKPIVLSLTGLMVQLIKLLTSKNR"
BASE COUNT          402 a          239 c          307 g          378 t
ORIGIN      
        1 atttaagttc agcagcagtt aaaacagcag gtgtacttat cagctttcag gctgtactat
       61 ccgcttatca gcgagtaatg gaagttggcc ttcaacgtgc aagtgctgaa cgttcaattg
      121 atttcgtatt ttctaaagat gcaaatcaag taaaagattt cacaaagtca ttagctcaaa
      181 caacgggttt agatatcgct gaactgcaat cacagtttgc tggtttcggt gcttcagcta
      241 gagagtctct aggcattcaa ggttctgaag aactttttag aaatatgatt ggctattctc
      301 gtctgatggg gcgaagtgaa gaagagatta agcgagcact gacagcatta tcacagatgg
      361 ctggtaaggg tcagattatg gctgaagagc ttaaggggca actagctgaa gctgttcctg
      421 gtatggttca ggtattcgct aaagcaaccg gaaagactga acaggaatta tttgatgcta
      481 tgaagaaggg ggctttaaaa tctgccgata ctcttcagaa ggtcactcag gaactgaata
      541 aacaaatcac cgctaaaggt ggctggaaag ctatttcaga aagtacacag gcacaactag
      601 ggaacttgaa aaactcttgg aataccacgc ttgatagtat cttcagaggt tctgaaaatg
      661 gtctgcaaga ttttacaaga agcttaacca atcttttaaa cgctcttggt ggaagtggta
      721 agagtcttgg tgaatctctt ggtagtctca tgacttctat gtcacatggt gtcgattcat
      781 tgaccgatat tagttacagg gtaagggcgt tctttgatga ggtgacatta gcttaccgtc
      841 cactaaatga cactcagagg aaaattgttg atggtttatc tggtgggtta attgatggtc
      901 tgaagatgct tgctgtggct gttactgctc aaaaggctgc aaatgtagca ggaggcttgt
      961 taaaccttgc aaacgctgtt acttcattcg gtgacgctgt taataaagct aaagctggca
     1021 aggctggttc ttcaggtggt gcgctaaata aagtcggtag agttgtcctt tatgctgagt
     1081 tggtaaattt tgctcttgat aatttaaacc ctgaagcagc taagaatccg aacttacatc
     1141 aatcaggtat tggcacagaa acaaacttca accctgaata caaaggtgat tcatctaagc
     1201 caatagttct atcgctgact ggtttaatgg tgcaactaat aaaattgcta acttccaaga
     1261 accgatgaat aacttcgtaa ataacacaca atcaactcca tcagctatca caggtgacat
     1321 ggttaa
//
LOCUS       DADLCQ010000317         1326 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000317 DADLCQ010000000
VERSION     DADLCQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.320"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..171)
                     /locus_tag="JQG95_004307"
     CDS             complement(<1..171)
                     /locus_tag="JQG95_004307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890622.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1326
                     /locus_tag="JQG95_004308"
     CDS             308..>1326
                     /locus_tag="JQG95_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890623.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSA"
BASE COUNT          349 a          329 c          380 g          268 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaa
//
LOCUS       DADLCQ010000318         1314 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000318 DADLCQ010000000
VERSION     DADLCQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.321"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(102..1253)
                     /locus_tag="JQG95_004309"
     CDS             complement(102..1253)
                     /locus_tag="JQG95_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HAY5890624.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          313 a          306 c          287 g          408 t
ORIGIN      
        1 cctctccgat aacaccagaa caaatcgaaa tacgtgagct gaggaaaaag ctacaacgca
       61 ttgaaatgga gaatgaaata ttaaaaaagg ctactgtaga ttcaatctgt caatgcaaca
      121 cccctttcaa ttatctcttt cggtgttttg aacttcagtg tctttctcgg tctgttgttt
      181 agctgagcag caaccagatc tagttcatgt tgagtatatt gggcaagaca tgtcttttta
      241 ggaaagtact gccgaattag cccatttgtg ttctcatttg ttccccgctg ccaaggactc
      301 tgaggatcgc agaagtaaac tttaacgccg gtgctgacag taaattctag atgtctggcc
      361 agttccattc ctctgtccca tgtcagtgat tttctgagtt ctgacggtaa actcaggaat
      421 ttgtcggtaa gagcctgatt tactgagaca gaatctttgc ccctgagtct gaggatgatc
      481 gtataacgtg attttcggtc tacaagtgtg gctatatgag agttttttgt acctgagact
      541 aaatcgccct cccaatgccc cagagagcgt ctgttatcga tatttcggga acgttcgtga
      601 attggtgttc cgttcactat gttaatcgta cctctttcgc ctttgcgggt atgacgcctg
      661 ccatggcgaa ggctatgcga ccgtcgcaga tgctgtatat tcaggtggtg tagcgcttca
      721 cggctacgaa agtacagcgt tttataaatt gtctcaggtg atattcgcag cgttttttga
      781 cgtggttttg ttcgccttaa ccatcctgat atttgctctg gagaccattt catctccagc
      841 ttttccagaa caagctttcg caatggtaaa ttttgatcca gtaagcacgg ttttggcctt
      901 ttcgccattc tgttggctcg gttattagca tcaacagctt tgtaatagcg tctgccccga
      961 ttacgctgaa cttcacgtga gatcgtcgaa ggactgcgat tcagcgcagt agctatcgca
     1021 cgaatgctca ttttggctga caaaccagct cgtatctcct cgcgctcaga cagtgtcagg
     1081 tgagctacag cccgcttacg ctcatggggt tttatgccgc cagtatccct taacatagtg
     1141 aagatcgttc cgggttttga acccaggata ttcgctattt cactgaagcc tgttccgttc
     1201 ttccatagtt caaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa
     1261 acctccgcaa ccccatgttt tcacataact gttgcgttga ccaattgaat ctac
//
LOCUS       DADLCQ010000319         1291 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000319 DADLCQ010000000
VERSION     DADLCQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.322"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            227..442
                     /locus_tag="JQG95_004310"
     CDS             227..442
                     /locus_tag="JQG95_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890625.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEI"
     gene            545..823
                     /locus_tag="JQG95_004311"
                     /pseudo
     CDS             545..823
                     /locus_tag="JQG95_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            825..>1291
                     /locus_tag="JQG95_004312"
     CDS             825..>1291
                     /locus_tag="JQG95_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890626.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGT"
BASE COUNT          320 a          322 c          331 g          318 t
ORIGIN      
        1 gaggagtaag agacccggcg agtgagaaat ccctcgccac ctctgatgtg gcaggcatcc
       61 tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt ttttattttc aacgcatttg
      121 aagttctgga tggtgccgga atagaatcaa aaatacttaa gtagcgcgca gggataaaag
      181 ggatggaccc cgaacagggg agtgctactt atctggaagg attctgatga tgaacatcga
      241 agaactgcgt aaaatttttt gtgaagatgg cctttatgct gtgtgcgttg aaaatggaaa
      301 tattgttagt cattaccgca ttgtgtgttt gcaaaaaaat ggggctgcgt taattaattt
      361 tgtggatgcc cgagtgacgg acggatttat cttgcgcgac ggtgagtttg tcacttcatt
      421 acaggcattg aaagagatct gaataaaagc tggcttttct gctttttcag aagaataaac
      481 tcatctacaa tcttgcgcgg ggctgaactc ccgctgagta acaccgtgcc accggagaaa
      541 accgatggca cgcaacgcaa aatattacaa tcatgataat tcgaccgttc ttgcccacac
      601 gcacgagcgg tattctcacg catttaagtc agactggtac cagcatccct catgcactga
      661 agaacaggcc gaatggctta ttcagtgtta ccgcaggcgc ggatgcgagg ttaaaaaagc
      721 ccttagcctc gactaccgtc actagataat ctccgtcagg ctcccttact ccgaacagcc
      781 accgcgtccg tcccgcacat tccagcaacg gatctggagg taacgtgcgg gtattacttc
      841 gacctgttct ggttccggaa ctcgggctgg tggtccttaa gcccggtcgt gaatccatgc
      901 cggtattcca caatacccgg gtactggtgg agccggaacc gaaaagcatg cgtaatctgc
      961 cgtccggggt cgttcctgcc gttcgccagc cgctagtgga agacaaaaca ttgctgccgt
     1021 ttttcagtaa cgcacgggtg attcgtgctg ctggtggtgc tggcgcattg tctgactggc
     1081 tgttgcgcca tattaaatcc tgccagtggc cacacggcga ttatcatcac agcgaaaccg
     1141 tcattcaccg ttatggtacc ggcgcaatgg tattgtgctg gcactgcgac aaccagctgc
     1201 gtgaccagac atccgaatca ctcgagcaac ttgctcatca aaacctgtca gcatggatga
     1261 ttgacgtcat cggtcacgca ataagcggta c
//
LOCUS       DADLCQ010000320         1287 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000320 DADLCQ010000000
VERSION     DADLCQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.323"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..70)
                     /locus_tag="JQG95_004313"
     CDS             complement(<1..70)
                     /locus_tag="JQG95_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5890627.1"
                     /translation="MEPVYVILNALLDSGRFTRKLIL"
     gene            522..806
                     /locus_tag="JQG95_004314"
     CDS             522..806
                     /locus_tag="JQG95_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY5890628.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHPLPVPR"
     gene            806..1081
                     /locus_tag="JQG95_004315"
     CDS             806..1081
                     /locus_tag="JQG95_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY5890629.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            1134..>1287
                     /locus_tag="JQG95_004316"
     CDS             1134..>1287
                     /locus_tag="JQG95_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890630.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQT"
BASE COUNT          339 a          323 c          326 g          299 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgcccgg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatccgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc cagacat
//
LOCUS       DADLCQ010000321         1281 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000321 DADLCQ010000000
VERSION     DADLCQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.324"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            82..1275
                     /locus_tag="JQG95_004317"
     CDS             82..1275
                     /locus_tag="JQG95_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY5890631.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          385 a          269 c          293 g          334 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta a
//
LOCUS       DADLCQ010000322         1271 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000322 DADLCQ010000000
VERSION     DADLCQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.325"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            482..>1271
                     /locus_tag="JQG95_004318"
     CDS             482..>1271
                     /locus_tag="JQG95_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY5890632.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGLMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAV"
BASE COUNT          294 a          292 c          279 g          406 t
ORIGIN      
        1 gacagcctat acattccgca gcttcagcag cgataaactt attcatttgc atccctttca
       61 tttaatgagt tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc
      121 atgaccgttt taattataat tttgcgactg agttcaaatt attgcccctc aatctatgcc
      181 gctttcatta tttcaacatt gccgatcaaa agagcgctat ccagataaaa acgattaaat
      241 gcgagagtgc gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa
      301 ttccattgtc aggttctctt gtcattttcg tggtttaagc acgattatgt gattgcctcc
      361 gcaattcctg tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt
      421 agacagtttc attaacgtaa acggttgctt tttattctgg cgggcgaaag gagaaacact
      481 gatgagcgcc atagattccc aacttccctc atcttctggg caagaccgcc caactgatga
      541 ggttgaccgc atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt
      601 catgtacgca ggtgcagtcg ctgttcctct tatgattggt gaccgactgg gcctctcaaa
      661 agatgctatt gcgatgctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt
      721 gcaatgtatc ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac
      781 ctttgctgct gtaacaccaa tgatagccat tgggatgaac ccggatatcg gcctgctggg
      841 gatatttggt gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttattgg
      901 tcgcttgatg cctttattcc cgccactggt taccggtgtg gttattactt ctattgggct
      961 tagcatcatt caggtgggta ttgactgggc cgctggaggt aaagggaatc cgcaatatgg
     1021 taatcccgtt tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg
     1081 ctatgcgaaa ggattaatgt ccaacgtcgc cgtattactg gggattgtat ttggcttttt
     1141 actttcgtgg atgatgaatg aagtcaattt atccgggcta catgatgcct cgtggtttgc
     1201 gattgtcacc ccgatgtcgt ttggtatgcc gattttcgat cccgtttcca ttctgaccat
     1261 gactgccgtg t
//
LOCUS       DADLCQ010000323         1266 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000323 DADLCQ010000000
VERSION     DADLCQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.326"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="JQG95_004319"
     CDS             complement(<1..715)
                     /gene="ipaH7.8"
                     /locus_tag="JQG95_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY5890633.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLST"
     gene            1044..1223
                     /locus_tag="JQG95_004320"
                     /pseudo
     CDS             1044..1223
                     /locus_tag="JQG95_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          415 a          184 c          252 g          415 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatattaa tcctgtaact tgaatctaat tgtgaaaaac
       61 tctccggaag agtggttatc tgattaaaat gaaaataata ttccttttgt ctgatattgt
      121 ttctatcaga aaattgagga aaagtaacca gattattgcg ctgagcacag agaatttcca
      181 gtttatccgg taagtcgggt aatgttgtca gcctgttata ataaacatgt aaaaatttca
      241 atttttcggg aagagccggt aagttatata gcctgttatt ttcaacaaat aatgatgtta
      301 aatggtttgg caaaacagga aggttttcca gttgattaga acgaacactt aaagttgtta
      361 atgactcagg aagttctggt atcacagaaa gcctgttatt atcagcatta agttctttaa
      421 gaaatgcagg cagctttggc aatgatgtta atttattatt ttctacaatc aaagcagaaa
      481 tatgctttgg taattcggga agagacgtca aatttaggtc tgataaattt aatactgctt
      541 cttgattctg aaagcactgg gagagtctgt taaaagcaat gcctcgctct tccccgggag
      601 aagagttctt ttcccattca gtcaggattc tcagataatg ttcattactg gttgagtttg
      661 agttaataga gggggagcaa gaaactgatg agtgtgtatt atttacagag aacataatgc
      721 taggtcaacc ttatatttgt gagaataaat atttaaaatt gtatatttat gcaaatttca
      781 cttaccattt ccgatattaa gttttaatag tttccatttt acacataaca gaagttagtt
      841 gtaatttatt tatggaggtt ttctatggaa ttcttttttt attgtgactg cagtcaataa
      901 taacactgaa ttcaacattc cagtgagcaa ggaaatgtaa tccgagtaag tatatttgtt
      961 ttacatcata ttttagtcaa gctcatttat tcctattata gaatgacaaa ttaaaattac
     1021 tgtaaaaaaa cgatacacat ttaatacgag ggaggccggc agaaaaacgg cttgcggtca
     1081 ggcaggagaa atgtgtgccg cgaatgaagt ccttgtatga ctggataaag ctccagagga
     1141 aaactttgtc aaaccatgct gaaatggtaa aggcgttcaa ctatatcctg aattactgga
     1201 atgcactgaa tgagttttgc cgggtagtac ttccaatcat tagaatatgt gcttttcaat
     1261 gaagga
//
LOCUS       DADLCQ010000324         1259 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000324 DADLCQ010000000
VERSION     DADLCQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.327"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            34..435
                     /locus_tag="JQG95_004321"
     CDS             34..435
                     /locus_tag="JQG95_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY5890634.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JQG95_004322"
     CDS             447..818
                     /locus_tag="JQG95_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890635.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGIFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1259
                     /locus_tag="JQG95_004323"
     CDS             805..>1259
                     /locus_tag="JQG95_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890636.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPV"
BASE COUNT          299 a          281 c          390 g          289 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatcttgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccggga tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtgga
//
LOCUS       DADLCQ010000325         1241 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000325 DADLCQ010000000
VERSION     DADLCQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.328"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>1241
                     /locus_tag="JQG95_004324"
     rRNA            <1..>1241
                     /locus_tag="JQG95_004324"
                     /product="16S ribosomal RNA"
BASE COUNT          312 a          285 c          392 g          252 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgctgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggtgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      721 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca c
//
LOCUS       DADLCQ010000326         1225 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000326 DADLCQ010000000
VERSION     DADLCQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.329"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="JQG95_004325"
     CDS             complement(<1..781)
                     /gene="ipaH4.5"
                     /locus_tag="JQG95_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY5890637.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLST"
BASE COUNT          349 a          220 c          264 g          392 t
ORIGIN      
        1 gagtcgacaa tggattccct gaaatgttaa cattcgtaca aatgggcaat gtgattatat
       61 tttcgggtaa tgaagttagt aggttattgg aaacatccag tgccaatagt tcttgcggta
      121 atctggataa tctgcgtagt tgattttcac ctaccttcaa atattttaag ctctgaggaa
      181 attctggtag ttcctgtagt ctgttgccgg aaacaaagag ttcttgtagt ctatgaggca
      241 attcaggcaa gacagtaagg cgattacctt caatacgtaa tattgttagc gttgatggaa
      301 gctctggtaa cgtctccaga ctattaaaac gtgccgagag tgattgtaaa tatggtggta
      361 atgatggcag acatgacaaa tttcgattga atgaaatatt aagcttaatc agttgagatg
      421 gaagtgtcgg gagtatattc aagttgttac catttacatg aagttctgtc aaaagcggag
      481 aattttcagg tagggagatt aactcattgt tggaaatatt aagttcacgt atatgcaaag
      541 ggagtggtgg taaagaacgt agtttcagta agcttaaatt gagaacaggc tcccgggtat
      601 ccagacatat tttcaataat cgaaccgcct ggatgcgatt ttctgttgtt gttccttccc
      661 tagcccagtc atcccagatg cggtggtaat ctctggtaca ctgttcttct accgataaac
      721 gagatataca tgataagcca caaagggaac gaaaaaaaga atgattgttg atcggtttca
      781 tttattaacc cctcactatt catatcttgg tctattaatg caaataaaac gagaaagtct
      841 ttaaaaaaac aatcctagaa ctttatcggc gttttcattc cggctggagg aatatgttcc
      901 tgtctttcca gtaaccgtcg attgttatat cagtctaccc atgttaatgt gaccagttct
      961 actttcatcc agtttggatt gatctttatc ccctatattt tcaaacaatt tttatcattt
     1021 aactcattac tgtctcgata ttcccatagc aaacgatctg gttggttatc ccctcaaaaa
     1081 agtcagttgc acgccgtaaa tccctgccgg tattgagcga ggatttacgg gttgttgtta
     1141 aggtccgtaa tcgggtcaca gtttttccgg agtcaatccg gtctgcggtt tatgcttatg
     1201 cgacgtgatt atgaatggtg cagtc
//
LOCUS       DADLCQ010000327         1189 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000327 DADLCQ010000000
VERSION     DADLCQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.330"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(267..1175)
                     /locus_tag="JQG95_004326"
     CDS             complement(267..1175)
                     /locus_tag="JQG95_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890638.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVEGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          228 a          324 c          289 g          348 t
ORIGIN      
        1 caatcagtat gatgacgagc ttcatcataa ccctttcctt ctgtaaggcc ccttcctcgg
       61 gaggggcttt cccgtttcag ccctttgctg actccccgat tgttgatgag cgccggacct
      121 cgcgcaacgc tttcccagag gcagttttgc cttccccgag cggtcacgca aaacgttgca
      181 acgacctgat catttgcagt ttttagcgga acgcagatat aaaaaatccc cggctttttg
      241 tgccagggac gttcatagct gaaaatttat tttacgtttt cggcgacggg agttgttgag
      301 attaagggtg aagtcaatgc cgccagtgaa ccgcctgaaa cgccaatttc attgagcaag
      361 gagtcaatca gcggagcctg tgtgcggtaa gagagagcgg ctgataatgc ttgttccgct
      421 aggtttccgc cctccacatt acccgtgttt gcatcacccg cagcgctgcc actattcaga
      481 ccatcgacct gaataatctt gataccgtca atagatttca tcggctcaac ggatttttct
      541 attaccgcag gtagcgcctg caacaaggcc agtttgaatt taaggctggt ttgttcatca
      601 gaaagtacgt tgatagcatc gttcagcgca cgttgtgctt ctgcttccgc cagacccttt
      661 ttacgtgtag cttcggctaa ctcaacgata gccgccgcct gcatctctgc ggcttctttt
      721 tctgctttcg cccgcacggt cagttcaacc gctttggttt ctgcatcctg cgcggcagcg
      781 attagggcaa cttgtttagc acgatcggct tctgcggtct ggcgagtggt ttctacgttt
      841 tgttgcgcac ttactgcttc tgcgagtgca aggttagcac gcgcttctgc ctgggactgt
      901 tgttccgatt tggcggcaat agcgatcgat ttcgtctggt tggcgatttc ggtgacctgc
      961 tgctgttcga tctctttaat acgaacttca cgttcagcct caacctttct tgagcggacg
     1021 gcctgctcgc gctcgatctc cgtttcctga atttgtcgtt cagccagaat tcgcgtctgc
     1081 tctgcttcac gacgacgttc agcttcaaaa gccgcaatac gtgcattctg ttcggcagta
     1141 cgggttttaa cctgctgctc ctgctcaagc gtcataaacg cttcttgtt
//
LOCUS       DADLCQ010000328         1159 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000328 DADLCQ010000000
VERSION     DADLCQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.331"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="JQG95_004327"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="JQG95_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAY5890639.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASAILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          245 a          255 c          299 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcagctatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DADLCQ010000329         1155 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000329 DADLCQ010000000
VERSION     DADLCQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.332"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            2..385
                     /locus_tag="JQG95_004328"
     CDS             2..385
                     /locus_tag="JQG95_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY5890640.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JQG95_004329"
     CDS             complement(386..1030)
                     /locus_tag="JQG95_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5890641.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAAMPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcgcgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcattgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADLCQ010000330         1137 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000330 DADLCQ010000000
VERSION     DADLCQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.333"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(16..99)
                     /locus_tag="JQG95_004330"
                     /pseudo
     CDS             complement(16..99)
                     /locus_tag="JQG95_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            381..>1137
                     /gene="torT"
                     /locus_tag="JQG95_004331"
     CDS             381..>1137
                     /gene="torT"
                     /locus_tag="JQG95_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY5890642.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTV"
BASE COUNT          278 a          260 c          294 g          305 t
ORIGIN      
        1 ccatttcggt cccagttagg acgctttctc atcaagccag tccgcccacc actgcatcat
       61 ctccctacgc ttatcgagat actgagcatg gttgtaaatt caattaaccc agtgcgttga
      121 caaaaaatta gcgcaagaag acaaaaatca ccttgcgcta atgctctgtt acaggtcact
      181 aataccatct aagtggttga ttcatagtga ctggatatgt tgtgttttgt cgcgtttttc
      241 agtctattat ttaatcatta agtaatctaa tgctatgatt tttaaattat ttatgtgatt
      301 ttgtcatcct ttaggtgaaa aaggttgagt cgcaaagtgg aatgcatcta gcataaagcc
      361 ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt
      421 tgtcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat ttcacggtgc
      481 aagcctctat gccgcttaaa gccaaacgcg tatggaaact gtgcgcgctt tatcccagcc
      541 tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg
      601 gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac
      661 aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt
      721 catttcccga cctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgctc
      781 ttgatgatcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg ggctatcaac
      841 ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg ctgatgcccg
      901 gacccgataa cgccgggggc agtaaggaga tggtcgaggg ttttcgcgca gccattgccg
      961 gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa atccagcgca
     1021 acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc ggaacggcca
     1081 ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt accgtgg
//
LOCUS       DADLCQ010000331         1125 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000331 DADLCQ010000000
VERSION     DADLCQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.334"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..114
                     /locus_tag="JQG95_004332"
     CDS             <1..114
                     /locus_tag="JQG95_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890643.1"
                     /translation="GPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            111..512
                     /locus_tag="JQG95_004333"
     CDS             111..512
                     /locus_tag="JQG95_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890644.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            523..>1125
                     /locus_tag="JQG95_004334"
     CDS             523..>1125
                     /locus_tag="JQG95_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890645.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSD"
BASE COUNT          284 a          279 c          328 g          234 t
ORIGIN      
        1 ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg
       61 aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa atgaaccgac
      121 acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga gacagcgcca
      181 cgttttttga cgggcttccg gcatttattg atgcgcagga actgcctgcc gtggcggtgt
      241 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
      301 tgcatattgc cgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
      361 agagcaccat tttcccggcc ctgaatgatg taccggcact ttccggactc atcgacaccc
      421 tgaacccact cggttttaac tatcaacgtg ataatgagat ggctacctgg gcgatggcgg
      481 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
      541 ctggcaaaga cgaaaggtgc ggggacgacg ttctggatgt ataccggcaa cggcgatgcg
      601 tttgcgaacc ctttgtcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
      661 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
      721 tggaaaacga caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg
      781 cgtccgggtg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
      841 gcgtttcgta tcaaatatcc caacggcact gttgatgttt tccgcggctg gctgagctca
      901 ctgggtaaaa ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg
      961 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gtgttaccgg actgacggtg
     1021 gcaccggcat ctgtcagtgt caaagcggga gcaaccacca cactgacctt tacagtaaaa
     1081 cctgacgggg ccagtgacaa agcgatcagt gtgcattcgt cagat
//
LOCUS       DADLCQ010000332         1110 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000332 DADLCQ010000000
VERSION     DADLCQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.335"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..1019
                     /locus_tag="JQG95_004335"
                     /pseudo
     CDS             1..1019
                     /locus_tag="JQG95_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1110
                     /locus_tag="JQG95_004336"
     CDS             992..>1110
                     /locus_tag="JQG95_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY5890646.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          313 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tagcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag
//
LOCUS       DADLCQ010000333         1098 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000333 DADLCQ010000000
VERSION     DADLCQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.336"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            48..>1098
                     /locus_tag="JQG95_004337"
     CDS             join(48..297,297..>1098)
                     /locus_tag="JQG95_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY5890647.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAIT"
BASE COUNT          302 a          271 c          309 g          216 t
ORIGIN      
        1 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgctccaatg aaaaaaagaa
       61 attttagcgc agagtttaaa cgcgaatccg ctcaactggt tgttgaccag aaatacacgg
      121 tggcagatgc cgccaaagct atggatgttg gcctttccac aatgacaaga tgggtcaaac
      181 aactgcgtga tgagcgtcag ggcaaaacac caaaagcctc ccccattacc ccggaacaaa
      241 ttgaaatccg tgagctcagg aaaaagctac aacgcattga aatggagaat gaaatattaa
      301 aaaaggctac cgcgctcttg atgtcagact ccctgaacag ttctcgataa tcgggaaact
      361 cagagcgcat tatcctgtgg tcacactctg ccatgtgttc ggggttcatc gcagcagcta
      421 cagatactgg aaaaaccgtc ctgaaaaacc agacggcaga cgggctgtat tacgcagtca
      481 ggtacttgag ctacatggca tcagccacgg ttcggccgga gcaagaagca tcgccacaat
      541 ggcaacccgg agaggctacc agatgggacg ctggcttgct ggcaggctca tgaaagagct
      601 ggggctggtc agctgtcagc agccgactca ccggtataaa cgtggtggtc atgaacatgt
      661 tgctatccct aactaccttg aaaggcagtt cgccgtgacc gagccaaatc aggtgtggtg
      721 cggtgatgtg acctatatct ggacgggtaa gcgctgggcg tacctcgccg ttgttctcga
      781 cctgttcgca agaaaaccag tgggctgggc catgtcgttc tcgccggaca gcaggctcac
      841 catgaaagcg ctggaaatgg catgggaaac ccgtggtaag cccggcgggg tgatgttcca
      901 cagcgatcag ggcagtcatt atacgagcag gcagttccgg cagttattgt ggcgatacca
      961 gatcagacag agtatgagcc ggcgcggaaa ctgctgggat aacagcccaa tggaacgctt
     1021 cttcaggagt ctgaagaacg aatggatgcc ggtggtgggt tacgtaagct tcagcgaggc
     1081 agctcacgcc ataacgga
//
LOCUS       DADLCQ010000334         1097 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000334 DADLCQ010000000
VERSION     DADLCQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.337"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQG95_004338"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JQG95_004338"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY5890648.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="JQG95_004339"
     tRNA            complement(986..1062)
                     /locus_tag="JQG95_004339"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          286 a          257 c          250 g          304 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt atgaccg
//
LOCUS       DADLCQ010000335         1091 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000335 DADLCQ010000000
VERSION     DADLCQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.338"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(299..>1091)
                     /locus_tag="JQG95_004340"
     CDS             complement(299..>1091)
                     /locus_tag="JQG95_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5890649.1"
                     /translation="SIRQACRSLNLSRTVYHYRPDTTRDEPVIVALQAVAERYPRYGF
                     PKLFQVLRRQGYPWNHKRIHRIYCLLKLNFRRKGKQRLPVRNPSPLATSEALNQSWSV
                     DFMHDALVCGRRFRTFNVVDDFNREALSIEIDLNLPALRVVRVLDRIAANRGYPVKLR
                     MDNGPEFISLTLAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLN
                     EVREITEKWLSEYNCERPHESLNNMTPEEYRQHHYLAGISKNAWN"
BASE COUNT          308 a          255 c          251 g          277 t
ORIGIN      
        1 caatataaat cgctgcaaca ataaacgtaa acctgcgatt atacctccta gcctaggata
       61 ttagacggta agagccaaaa gacacaaaaa atgtaaaaaa ctgtaggtgt tctcatggtt
      121 cattacaaac gttgaatata caatgcaacc aatgtcattt gagcagtaaa aaacatgaat
      181 agcgtacaaa tagaagaaaa tgttaagagc ttggtaaaac aagtatcaag ccaagaaatc
      241 tcaaaagatg attttatcta cgagctattg ttggcttacg ggtgtaaata gacccgtttt
      301 agttccaggc attttttgag atcccggcca aataatggtg ttgtcgatat tcctctggcg
      361 tcatattgtt cagtgattca tgcgggcgtt cacagttata ttctgacaac catttttccg
      421 tgatttcccg cacttcattc agcgttctga acagataaaa atcgagtatt tctgtacggt
      481 atgtccggtt aaagcgctca atgaaagcgt tctgcgtcgg cttacccggc tggataaact
      541 ccagttttac tgcatgtttc tctgcccatt cagccagtgt cagggagata aattccggac
      601 cattatccat gcgcagcttg accggatagc cacggtttgc cgcgatcctg tcgagtacac
      661 ggaccacgcg tagagctggc agattcaggt cgatttcaat cgacaacgcc tcacggttaa
      721 agtcatcaac gacattgaac gtacgaaaac gacggccaca gaccagggca tcatgcataa
      781 aatcgacaga ccaactctgg ttcagcgctt ccgacgtggc cagtggcgag ggattacgca
      841 ccggcaaccg ttgtttgccc ttacggcgaa aattcagctt cagcagacag taaatacggt
      901 ggatcctttt gtgattccat gggtatccct gtcgccgcag aacctggaag agtttcggaa
      961 aaccgtatcg tgggtatcgc tctgccactg cctgcaacgc gacaataacg ggttcatcac
     1021 gcgtggtatc cgggcggtaa tggtaaaccg ttctgctcag gttcagactc cggcaggcct
     1081 gacggatact g
//
LOCUS       DADLCQ010000336         1070 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000336 DADLCQ010000000
VERSION     DADLCQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.339"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            190..513
                     /locus_tag="JQG95_004341"
     CDS             190..513
                     /locus_tag="JQG95_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890650.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            572..814
                     /locus_tag="JQG95_004342"
     CDS             572..814
                     /locus_tag="JQG95_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890651.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEICDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
BASE COUNT          260 a          239 c          314 g          257 t
ORIGIN      
        1 taaaaaattt tgcgttctgc ccaggacagg tgcgtcaggc cgtggcagtg atgccccttg
       61 cggcgaaggt tctcaaaaag gtgatggtga ataccggagg gaacaaaagg caggtccccg
      121 caaaacgtca aaaccgcggc tcccgaaact caaaagatga tgggggcaaa aaacagaggt
      181 gtattaaaga tgggcagaaa tatgtcagca gcacgtaccg atggctttat tcgtaatatt
      241 cattcccgca atccctttga tgttatccgg gctgatgtgg tgatcagcag gctggaaaag
      301 caggctcatt ggggatgtgg tctgcactat gaaatttatg aagcgaattt gtttgatatg
      361 gcgatgaatc atctttcccg gcttcctctg aaggatcgtc cggtattcat tggtgctgct
      421 gcacgaaagg ggctttttct gacgatggaa gaaaccaggc tggcacggga agcgaaagat
      481 gctctgatgg ctgaacttgc tctggaatac tgaaaatcgg gccgcaccgt cgtcccggat
      541 agtgctgaaa tatttaatgt ggagagaatg gatgggatat atcacatatg ttacagatca
      601 acgacccggt gaaccagata tcctgaccgg gaacacgttt gctgatctgg agatttgtga
      661 ttctgatggg catcttctgc tgaaagtgtc tgcacctgaa gccggctgga cacacgaaag
      721 cctgaacctt gttcagccgc aggaagtgca ggaggggaat gatgcatttg atgcttatct
      781 gaatggtata tggatcggtt cgacagaggt ctgaacaaat cacgccagca gaagacgttg
      841 ctggcgtctt ctgcattccc ggggagctgt cgcgcggcat gtttgcttat acccgtgccc
      901 cgagatgatt atttgtctgc gggcctgaag acgccgggca ggcgggagga taaatgcccg
      961 tctgcccgcg ccgggaaaag acagcacgcc ggtggcgctg aaagggggcg cgcttacgct
     1021 ccgccccttt cattacgcgg atgttctgct gtgtgcttct gtcttttcat
//
LOCUS       DADLCQ010000337         1070 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000337 DADLCQ010000000
VERSION     DADLCQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.340"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            20..1070
                     /locus_tag="JQG95_004343"
                     /pseudo
     CDS             20..1070
                     /locus_tag="JQG95_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          315 a          202 c          257 g          296 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
      781 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      841 atgtaaacgg aggattttct ttctcaaact catggatagc agcagatgcc gatggcactg
      901 aacaatttac ggggatatat tttagaacgc cggcctcaac gcagtcacat aaaattgtca
      961 gtggtgttca tatcaacact gcaaacaaaa acacggctgc aaacaatcaa agtatagcga
     1021 tagaacagtc ggcgatcttc gtctttgtaa gtggttgtac gttaactggt
//
LOCUS       DADLCQ010000338         1066 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000338 DADLCQ010000000
VERSION     DADLCQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.341"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(570..749)
                     /locus_tag="JQG95_004344"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="JQG95_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            843..>1066
                     /locus_tag="JQG95_004345"
     CDS             843..>1066
                     /locus_tag="JQG95_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890652.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DADLCQ010000339         1040 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000339 DADLCQ010000000
VERSION     DADLCQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.342"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..769
                     /locus_tag="JQG95_004346"
     CDS             <1..769
                     /locus_tag="JQG95_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY5890653.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKPG"
     gene            790..>1040
                     /locus_tag="JQG95_004347"
     CDS             790..>1040
                     /locus_tag="JQG95_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY5890654.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          337 a          231 c          234 g          238 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa ccggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
     1021 ttactgactt acctgcaaag
//
LOCUS       DADLCQ010000340         1031 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000340 DADLCQ010000000
VERSION     DADLCQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.343"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>1031
                     /locus_tag="JQG95_004348"
     CDS             <1..>1031
                     /locus_tag="JQG95_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY5890655.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAE"
BASE COUNT          319 a          193 c          269 g          250 t
ORIGIN      
        1 tgtctataac cacgaaccgt tatgcatcag gttatgccac actgactgaa gcagtaagtg
       61 cacaggatga acggaacaga aaaagagata agaatagtca cgatggctct acgatatcct
      121 tatcccagcc ccttggtaat atcgggaatt tgaacttcaa taccacgagg tataattctt
      181 cccggggcac aggaaacacg cggtctacca gcctgagtta tagtacggta tggagaggca
      241 taacattctc cattaactgg gcaaaaaacg acttgctgac atcgcataaa tggaaagtag
      301 atcggaaatt aagcgtgggt atttcagtgc cattatcgct gggtgacgaa aatcaaattt
      361 atgcgtcttc acagatgagt cgttcgggag agcagggtaa taactatcag gtttcattat
      421 caggtcagaa tagtggtggt gtatggtggg atgtagcgac aaatataact aacgctcatc
      481 agtcacaacc gaagagcacg atgaacattg tgcaagttgg caaaaatgga tcatatggtc
      541 agtttagtag ccattacagc tccagcgaaa acatgaaaca gctaggtgca aatttaagcg
      601 gtgggatact tattacaagg gatggactta catttggtca aaatgtggat gggacattag
      661 cccttataga ggcacccgga gcaacggggg ttaacgttaa tggctggccg ggactgagta
      721 ctgattttcg tgggtatgca atactgccag tgcagccata ccgaagagac gatgttattc
      781 tggatgaaaa aacgataggt aagaattacg acttaccaca aactagtcaa ttggttgtac
      841 cgacagctgg agccgttgtg cctgcgacat tggcagtgaa gagcggggat aaaggattag
      901 taaccttaaa acagaaagag ggtaaacccg tccccttcgg cgctgtgatt agttatagca
      961 aagacactga aaatatggcc ggcattgtag gtgaagatgg tattgcttat gtttctggct
     1021 tgagtgcgga a
//
LOCUS       DADLCQ010000341         1020 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000341 DADLCQ010000000
VERSION     DADLCQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.344"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(50..190)
                     /locus_tag="JQG95_004349"
     CDS             complement(50..190)
                     /locus_tag="JQG95_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890656.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="JQG95_004350"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="JQG95_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY5890657.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          206 c          237 g          281 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt ccccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DADLCQ010000342         1003 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000342 DADLCQ010000000
VERSION     DADLCQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.345"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            102..524
                     /locus_tag="JQG95_004351"
     CDS             102..524
                     /locus_tag="JQG95_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY5890658.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            525..938
                     /locus_tag="JQG95_004352"
     CDS             525..938
                     /locus_tag="JQG95_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890659.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPIKEDDPRNQ
                     VNFLYKPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEEQERRRNIEKLIQIERNAKARIRRATKSRTE"
BASE COUNT          288 a          201 c          276 g          238 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtt aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtcc gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccga taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta taagccatcg gaaagaccat attgccatca cgcctctgta cgggttgacg
      721 aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcggt tgtggagccg tttgactgga
      781 tgctctctgt ggcaaaaaga gaaaccagac tggcagatga tgtaaggcta ttgcgccagg
      841 aggaacagga aaggcggaga aatatagaaa agctgataca gattgagcgt aacgcgaaag
      901 cgcggatacg cagggcgaca aaatccagaa ctgaataatt aaatttagca ctgttaaaaa
      961 tttaatcctt aaccggaggg atttctgcac cctcaaatca tca
//
LOCUS       DADLCQ010000343          939 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000343 DADLCQ010000000
VERSION     DADLCQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.346"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            268..>939
                     /locus_tag="JQG95_004353"
     CDS             268..>939
                     /locus_tag="JQG95_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5890660.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLT"
BASE COUNT          228 a          236 c          264 g          211 t
ORIGIN      
        1 atggttcgcc aggtgggaac aggcaagaac ttcccgggcg acgccatgaa gcgtggctac
       61 gtgaccgtta atctcggttc tgattttgat gccagcacta tcaaaaaagg cgatctccac
      121 tgatgaatcc atcaaagtgc ctctgggtgg attcatgtcc acgtcagtca gtggcaaaaa
      181 cgtggtgctg accaacgctg aattcacagg tgccggtgat gctgacggca atgcagaaat
      241 ttcctggaag atttaaggaa cagacgaatg attacttttg atcaggcaac cgttgacagc
      301 tctggtgcct ttctcatcgg ggagctggag agactcgacc agacgctgaa cctgccactg
      361 gtggggtaca cctggagccg cgatattcaa ctgcgtgaag acgcctccat cgcagatgac
      421 atttccagct ggacgaatac cagcttcgcc gctgcgggta ctggcgcaaa tccgaatgga
      481 aaaaactggg taggcaaaga ctcaaccgct attgctggcg tgaacgtgga tatcggcaaa
      541 tccggtaacc cgctgaacct gtgggggatg gaacttggct ggacggtcat agaattgcag
      601 gctgctcagc aggtcggccg cccgattgat acgcagaagt atgacggtat gcaactgaaa
      661 tggcagatgg ataacgatga acaggtatat gttggcgatt ccgcattaaa cctgaaaggc
      721 cttgttaccc tggacggcgt gcctgtcaac aacgctgcca aaacgtgggc aacctcaaca
      781 ccggacgaaa tccgcgcaag cattaaccag gtgctgtctg atgcgtgggc cgcttctggt
      841 tactctgtgg ttccgcgtga tttgctgatc ccgcctgagc agtttgctct gttgtccagc
      901 atcatcgttt catctgcagg taaccagtcc ctgttgacg
//
LOCUS       DADLCQ010000344          933 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000344 DADLCQ010000000
VERSION     DADLCQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.347"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            47..319
                     /locus_tag="JQG95_004354"
     CDS             47..319
                     /locus_tag="JQG95_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890661.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCIWKAIYRSTSHYGKNVLLLQLA"
BASE COUNT          245 a          180 c          217 g          291 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc atctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DADLCQ010000345          927 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000345 DADLCQ010000000
VERSION     DADLCQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.348"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            198..569
                     /locus_tag="JQG95_004355"
     CDS             198..569
                     /locus_tag="JQG95_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5890662.1"
                     /translation="MVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTSYNAIEWPASPEISS"
     gene            593..>927
                     /locus_tag="JQG95_004356"
     CDS             593..>927
                     /locus_tag="JQG95_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890663.1"
                     /translation="MSVVISGALTDGAGISMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVA"
BASE COUNT          267 a          196 c          240 g          224 t
ORIGIN      
        1 gtataacgta gcgagtattt aacaatgttg catttaaaaa atattactac gggtaatccg
       61 aaaacagcag aacaatatca gatgacaaaa cgatattcgg tcacctggct tttttcagaa
      121 gacggaaaaa actggtatga agagctgaag aatttcgcca ggacacaatt aaaataactt
      181 acaccggaga cggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatt gaaccccgta
      241 acgccagtgt tattgaagtt cctgatatta ccgccaaccg ccgcattacc gcgccgggtt
      301 actggtttta ccgcaatgat gaatttgtct ttgactacag actcaaagcg gaagatgagc
      361 gtgatgccct tctggctcag gtcagtgccc ggacagggga atgggaagaa gacctgttgc
      421 tggggctaat cagcgacgaa gaccgggaaa aactgaaagc gtaccgcatt tacgcgaaat
      481 cgctgcaggc gatggatttc agtgtcatca ctgataaaac ctcatacaac gccattgaat
      541 ggcccgcttc tccggaaatt tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt
      601 agtgatatca ggtgcgctga ctgatggcgc aggtatctcc atgtccggat accatattat
      661 tctgaaatcc cgggtaaaca ccccggaagt ggtgatgcac actgttgctg atgtgatgac
      721 aggaaacgat ggtgaatact gtttccatgc gcggactgga aaatatggtg tgtatctgaa
      781 acaggactgg cacaacgagt acaacgttgg cgacattgct gtatatgagg actcaaagcc
      841 cggtacgctg aatgactttc tgattgctcc tgatgagggc gacctgaaac cagatgtcgt
      901 caaacgcttt gaggaaatgg tggcgca
//
LOCUS       DADLCQ010000346          927 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000346 DADLCQ010000000
VERSION     DADLCQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.349"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..927
                     /locus_tag="JQG95_004357"
                     /pseudo
     CDS             1..927
                     /locus_tag="JQG95_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          214 a          234 c          301 g          178 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatgcgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgta
//
LOCUS       DADLCQ010000347          910 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000347 DADLCQ010000000
VERSION     DADLCQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.350"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            30..509
                     /locus_tag="JQG95_004358"
     CDS             30..509
                     /locus_tag="JQG95_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890664.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
     gene            497..730
                     /locus_tag="JQG95_004359"
     CDS             497..730
                     /locus_tag="JQG95_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY5890665.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            730..>910
                     /locus_tag="JQG95_004360"
     CDS             730..>910
                     /locus_tag="JQG95_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890666.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWI"
BASE COUNT          249 a          212 c          241 g          208 t
ORIGIN      
        1 tcatcaaact gtcttggata gaagccgcag tggatgcgca caaaacgtta aatttcgaac
       61 ccagtggaag aaagcgtatt ggctttgacg tggctgacag tggtacagat aagtgcgcta
      121 acgtttaccg tcacggatcc gttgttttct gggccgacga atggaaggcc aaagaagatg
      181 aattactgaa gagctgccag cgtacttatc aggcggcgct ggagcgtgaa gcagatattg
      241 tttacgactc tatcggtgtt ggtgcgtctg ccggtgctaa attctctgaa attaacgctg
      301 accggaagag cgagaacgca tacgcgcgac gtgtgaatta ccagaggttt aacgccggtg
      361 ctggtgtgca tgagccagat gacgagtaca acggcatccc caacaaagac tttttcgcaa
      421 atcttaaggc tcaggcatgg tggctgaccg tttcagaaat acgtttaacg ccattaacaa
      481 cggagaacag tatcctgtgg atgagctgat cagcatagat tctcgttgtc cgttgcttga
      541 aaagctgaaa ctggaactga caacacctca tcgtgatttc gaccgtaacg gacgtgtgat
      601 ggtcgaaagt aaaaaagacc tcgcaaaacg cgagataccg tcaccaaacg ttgctgatgc
      661 attcattatg gccttcgcgc caatcgatac atcgctggat atctgggaac agctggggag
      721 acaggcctga tggcacgaaa caaacaagcc ctgcggcgaa ctgtgcaggc cacagctgat
      781 ggttatgaga attttattgc ccgagtaggg atgcagacac ctaaccagca ctcagcatcc
      841 acctaccggg ctaatttcac cagtcgtaac cgcatgctgg tggaatggtc ctatcgttca
      901 tcctggatca
//
LOCUS       DADLCQ010000348          893 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000348 DADLCQ010000000
VERSION     DADLCQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.351"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..224
                     /locus_tag="JQG95_004361"
     CDS             <1..224
                     /locus_tag="JQG95_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY5890667.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="JQG95_004362"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="JQG95_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY5890668.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..>893
                     /locus_tag="JQG95_004363"
     CDS             518..>893
                     /locus_tag="JQG95_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY5890669.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVE"
BASE COUNT          265 a          171 c          227 g          230 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agc
//
LOCUS       DADLCQ010000349          879 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000349 DADLCQ010000000
VERSION     DADLCQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.352"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>879
                     /locus_tag="JQG95_004364"
     CDS             <1..>879
                     /locus_tag="JQG95_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY5890670.1"
                     /translation="FSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLLQI
                     TENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTG
                     QRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDLLT
                     VLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDFDT
                     PWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSPTD
                     DDELPEVTGPAWIVDNV"
BASE COUNT          195 a          203 c          276 g          205 t
ORIGIN      
        1 ttttctggtg aaccggcaca gatcgctgag attaaacgac tggccagcgg tgcagtcaca
       61 ccgctttatc gccgcgccac aaatgaaggt attcagctgt ttctggccgg aagtgccgga
      121 cttctgcaga tcactgaaaa tatccgctct gagcagtgcc ccggagtgac ggctgccgga
      181 cgtggtgctg tatcactgga aaatatcgcg ttcacccgct ggctgacaca cctgcagaac
      241 ggtgtactgc tggatgagca aaactgcctg atgctgcatg aactctggct gcagagcggt
      301 actggacagc gtcgctggga agggttaccg gatgatgtca gggaaactat cactgtgcat
      361 ttcaccgcaa aaagaggtga ctggtgtgac atctggggta acgaggatgt atcggtgtgg
      421 tggaaccgtc tgtgtgacaa cgtactgccg gaaaaaacca tgccgtttga cctgctgacg
      481 gttttgccga cccgcctgga tattgaagtg aatggcttta acggcggtgt tctgaacggt
      541 gttccttctg catatcactg gtatacagaa cggtatggcg tgaagtggcc ttgtgggtat
      601 gacctgaata tcagcagtca gggggaaaac ttcattcagg tggatttcga cacgccgtgg
      661 tgtcagccgg aaagcgacgt tgttgcagaa ttaagccgcc gtttcggctg cacgctggag
      721 cactggtacg ccgaacaggg ctgcaatttc tgtggctggc agttgtatga gcgcggagag
      781 cttgttgatg tgctgtgggg ggaactggaa tggtcttccc cgacagatga cgatgagctg
      841 ccggaagtca ccggacctgc gtggatagtc gacaatgtg
//
LOCUS       DADLCQ010000350          867 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000350 DADLCQ010000000
VERSION     DADLCQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.353"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            75..572
                     /locus_tag="JQG95_004365"
                     /pseudo
     CDS             75..572
                     /locus_tag="JQG95_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            613..804
                     /locus_tag="JQG95_004366"
                     /pseudo
     CDS             613..804
                     /locus_tag="JQG95_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          223 a          216 c          255 g          173 t
ORIGIN      
        1 cccggggcgg ttcaaagcgc cagccccgtc ccgcccgata gagcgcggca tagcgggccc
       61 cggattactt gcgtgcgacg gcagaagaag ccctgaagcg aatcagtgaa ctgtacgcca
      121 tcgaggatga aatacgggga ttaccagagt cagagtgtct tgcagtcagg cagcagcgaa
      181 gcaaagcgtt actgacgtcg ctgcatgaat ggatggtgga gaagaatggc acgctgtcga
      241 aaaaatccag actgggcgaa gcattcagct atgtactgaa tcagtgggac gccctctgtt
      301 attacagtga tgacggtctg gcggaggcgg acaataacac agcggaaaga gcgcttcgtg
      361 cagtctgtct cggaaagaaa aattacgtgt tcttcggtag cgatcacggc ggcgagcgtg
      421 gtgcactgct gtacgggctg atcggcacct gccgtctgaa cggtatcgat ccggaagcgt
      481 atctgcgcca tattctgagc gtactgccgg aatggccttc caaccgagtt gacgacctcc
      541 tgccatggaa ggtggtgcta ccatcaggat aatcatcaat acggtgttcg gtgaacgttt
      601 acttggagca tcatgcttca tgaagaaaaa ctggcacgtc atcaacgcaa acaggcgatg
      661 tatacccgaa tggcagcctt cccggcggtg aaaatgttcg aagagtatga cttcacgttc
      721 gccaccggag caccgcagaa gcagctccag tcgttacgct cactcagctt catagagcgc
      781 aatgaaaaca tagtcttact ggggacatct gacatcacaa atccccgggt ggggatttgt
      841 gtataagaga cagcccatgg aggaagg
//
LOCUS       DADLCQ010000351          861 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000351 DADLCQ010000000
VERSION     DADLCQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.354"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..254)
                     /locus_tag="JQG95_004367"
     CDS             complement(<1..254)
                     /locus_tag="JQG95_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890671.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="JQG95_004368"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="JQG95_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY5890672.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="JQG95_004369"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="JQG95_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY5890673.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          239 a          174 c          219 g          229 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg a
//
LOCUS       DADLCQ010000352          860 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000352 DADLCQ010000000
VERSION     DADLCQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.355"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..215
                     /locus_tag="JQG95_004370"
     CDS             <1..215
                     /locus_tag="JQG95_004370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890674.1"
                     /translation="CADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            263..692
                     /locus_tag="JQG95_004371"
                     /pseudo
     CDS             263..692
                     /locus_tag="JQG95_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          212 a          171 c          271 g          206 t
ORIGIN      
        1 gttgcgcaga cctgaatatt ccgttctttg tggatgccgg aaacaaaaaa ctggtcttct
       61 ggtttgtacg tgttgatgac gaagggtatc cggaaattgc ccgctgcatg gagcgggaat
      121 ttgcgacaat tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg
      181 ttcactggcc ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt
      241 tcagcaggga aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct
      301 ggaacattat gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg
      361 agaagaaaac cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc
      421 ggataagggg ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg
      481 aagatgtggt gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga
      541 gagggatgta tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc
      601 agatgcggtt tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg
      661 agccgctggc tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg
      721 ttgaggggaa cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata
      781 ttaagtgtct ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg
      841 gtgctttttt aatcttttgg
//
LOCUS       DADLCQ010000353          831 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000353 DADLCQ010000000
VERSION     DADLCQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.356"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..326
                     /locus_tag="JQG95_004372"
     CDS             <1..326
                     /locus_tag="JQG95_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY5890675.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQG95_004373"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JQG95_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY5890676.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          253 a          183 c          154 g          241 t
ORIGIN      
        1 ttagtgaaac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DADLCQ010000354          827 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000354 DADLCQ010000000
VERSION     DADLCQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.357"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            218..430
                     /locus_tag="JQG95_004374"
     CDS             218..430
                     /locus_tag="JQG95_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY5890677.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          231 a          158 c          240 g          198 t
ORIGIN      
        1 gaagcgtatg cgggagcgcg tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa
       61 cttcaggccg tgcttgatga gaaataaaaa aagcctcccc cagagagagg aggcagggaa
      121 ataaggctta aaaggaaata acttccgctt ccgaaaggaa gccagtccac tgaattattt
      181 atcacaataa tactgacaac taaaggggga caagaaaatg aatggattca gaaacagttc
      241 acggaacggc caggtctggc gttaccagcg tgccggcggg cgggccgtta ttctggaagt
      301 cagtggacgc tggatggaag cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag
      361 gacagactgg cagcagtttg cccggaaaag ggctgaacac tgccaccggc gctgccgggg
      421 aagggtgtaa cgaaaaaagc agccttcatt actctaggag tcagaaggct gaaaggagtt
      481 tatgagattt gcttcttaat atccggtcgg gcagtatgac cggacagttc tgagtagcac
      541 agagtctttt ccgcattaag tcgatttttt atattttgtg attagtttca caaaataagg
      601 tgttattcaa tgtgtgctgc actattcagg ggggctgaaa tacaggtggc atttatctga
      661 tatggagaat aacatgagga aatatattcc actggtatta tttatctttt catggccggt
      721 attgtctgca gatattcatg gacgggttgt tcgtgtactg gacggtgata ccatcgaggt
      781 catggactca ctgaaggcgg tgcggatcag gctggtaaat attgatg
//
LOCUS       DADLCQ010000355          824 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000355 DADLCQ010000000
VERSION     DADLCQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.358"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..537)
                     /locus_tag="JQG95_004375"
     CDS             complement(<1..537)
                     /locus_tag="JQG95_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890678.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASM"
     gene            545..>824
                     /gene="tnpA"
                     /locus_tag="JQG95_004376"
     CDS             545..>824
                     /gene="tnpA"
                     /locus_tag="JQG95_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY5890679.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGIS"
BASE COUNT          208 a          199 c          189 g          228 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 attccggtag cgcatccagc caagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgtcc tgcgggtagc ggaatgcagc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttctggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggcgaat ctttaagcca ttgcgtttca gtggcgtttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acgctcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccaaatac cgacgccaga tttttgacca
      661 tgacacgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt ttgcagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaact atcctcccaa
      781 actggcgata tccagtctgg taaacagcct caaaggcata tcga
//
LOCUS       DADLCQ010000356          815 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000356 DADLCQ010000000
VERSION     DADLCQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.359"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            92..565
                     /locus_tag="JQG95_004377"
     CDS             92..565
                     /locus_tag="JQG95_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY5890680.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          253 a          163 c          177 g          222 t
ORIGIN      
        1 ctgggggcag taatcatgcc gggtctggct ggctaacacc ccattataag gggtacgaat
       61 aaagtaacgg gggggagatg aaccctatgc aatgtcctgg ttaatatctc agtgtgctca
      121 tcagtgcact gataataaaa agacggagac agatgccatt tacgataaag ttcggagttc
      181 ttatttgctt tcatgtattc tgaaaaaaaa caaaaatgtt gggcttattc ttcatgcacc
      241 atcatttgtt tctgtgtctg aaaaaatcgc cagaattgtg atggcaaatt actcccgtaa
      301 ctggagtaat tccgagttgg ccagtgcggt tttaatgagt gaatcttctt taaaaagaag
      361 aatgtacaaa gaggtcggtt ctataagtac atttgtacat aaaataaagc ttacagaagc
      421 tatcaggaaa ttacgaagga caaatactcc catttctgtc atatcatctg agttaggtta
      481 cagttcacca tcgtattttt ctaaagtctt ctttaaatat ttaaagacat accctcaaaa
      541 cataaggaag aaaaatggtc ggtaatgtca ccaattagga ggacttgcag agccgctttt
      601 ttacgatact gagatcaaac agacataatc tatgcggaga tttacagcag ggcatggttc
      661 acaaatctga cagcgatgaa ttatcggcac tgcgggcaga aaatgctcga atcatcaagc
      721 ccctgttacc tcctgaaccc gccacaccac gggccggacg cccatgggct gagcaccgta
      781 aaatcatcaa tggcatgttc tgggtgttat gttcc
//
LOCUS       DADLCQ010000357          814 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000357 DADLCQ010000000
VERSION     DADLCQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.360"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..537)
                     /locus_tag="JQG95_004378"
                     /pseudo
     CDS             complement(1..537)
                     /locus_tag="JQG95_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            545..769
                     /gene="tnpA"
                     /locus_tag="JQG95_004379"
                     /pseudo
     CDS             545..769
                     /gene="tnpA"
                     /locus_tag="JQG95_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          208 a          197 c          185 g          224 t
ORIGIN      
        1 cattgatgct gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct
      121 attccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcatc attctgtcac cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acactcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tagtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaact aacctcccaa
      781 actggcgata tccagtctgg taaacagcct caaa
//
LOCUS       DADLCQ010000358          813 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000358 DADLCQ010000000
VERSION     DADLCQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.361"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..326)
                     /gene="traX"
                     /locus_tag="JQG95_004380"
     CDS             complement(<1..326)
                     /gene="traX"
                     /locus_tag="JQG95_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY5890681.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAG"
     gene            complement(346..>813)
                     /locus_tag="JQG95_004381"
     CDS             complement(346..>813)
                     /locus_tag="JQG95_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer nickase/helicase TraI"
                     /protein_id="HAY5890682.1"
                     /translation="GVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTA
                     EVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQERDRAAITE
                     REAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
BASE COUNT          146 a          271 c          201 g          195 t
ORIGIN      
        1 aagcctgcca gataatatgc gaactgggca ataattcccc atccccacag ccggttaatg
       61 gccggttgcc ggatatgcgc atgacgggac agattcagcc cccacaccag ggcaaaaagc
      121 ggaaaggctc ctcgcccggc cagaaacatc cattcctgct tcagctggaa tatcagattg
      181 atgtggtcca gtaccatcag caccagtgcc accgttttga tgatatcccg ctgtccgggt
      241 gaccagacca gaaatgactg caaccaggta tcggtccgtg cagccagtga atcgttacgt
      301 gtcgtgttcg tgttatccgt tgtcatcagt gtttgtcttc ctgtatcagt ctccgcccgg
      361 ggttttctct ttctgcaggt cacgaaccat atcccgctcc atctgctgca gacgctcctg
      421 cagcaaattt tcccgggcaa cttcccggac cgcttcccgc acccgttgtg gttcacgcag
      481 tacactctcc ggcagcgcgg cttcccgttc agttatggca gcccggtcac gctcctgccc
      541 ggcaatttcc ctgacagcct gctctgtttt gccgtccggc aggtcaggtt tgttctctgc
      601 cattttacgg acaatttcat ctgcgcgacg ttccgtttca cggcggatgg cctcttcagc
      661 ctgccgctgt gccagtacct ctgccgtgac cggttcacca tttccggctc ccggctggac
      721 gctgttgtcc gggatatccc cccacacgcg gccaccggtg atggcaccgg gattccacgg
      781 acggccttca ccggcgattc ttaccaccac acc
//
LOCUS       DADLCQ010000359          811 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000359 DADLCQ010000000
VERSION     DADLCQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.362"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>811
                     /locus_tag="JQG95_004382"
     CDS             <1..>811
                     /locus_tag="JQG95_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY5890683.1"
                     /translation="KKLDVSCNKLTILTDLPENIKELIARDNFLTHISALPHYLITLD
                     VSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLKKLEVRNNELQTLPSLPSNLKI
                     LKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQENIIMRRFFYFENNQITTIPTNL
                     FRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISLSDQNRLFLRQMLPQNL
                     HSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTSFSTEENSRA"
BASE COUNT          259 a          187 c          122 g          243 t
ORIGIN      
        1 ttaaaaagct tgatgtgagc tgtaataaat taaccatcct tactgattta cctgaaaata
       61 ttaaagaact tattgcaaga gataatttct taacacatat atctgcatta ccacattatc
      121 taataacttt ggatgtgtcc gaaaatcaat tagagaatct gccgttatta ccagacacca
      181 tcaaatcact aagcgcagag tataataggt tatccacact gccttcatta cccttgaatt
      241 taaaaaaact tgaggttagg aacaacgaac tgcaaactct tccatctctg ccttctaatc
      301 ttaagatact taaggttgcg cacaaccatc ttactgaact gcccccttta cctaggagac
      361 tgcaacttct ttttgcatat agcaatagat taagcaactt accaaacatc caagaaaata
      421 ttatcatgag aagatttttt tattttgaaa acaaccaaat aactacaatc ccgacaaatc
      481 tttttcgttt agatcctcat ataactattg agattgcaaa taacccctta tcagatcaaa
      541 ctctgctatt cttaatacag caaacttcgg ttccaaattt taacgggcct cagtttcgta
      601 tttccctgtc agaccaaaac agactgtttt tacgccagat gttgccgcaa aatttacatt
      661 cgcgccatat cagagtcatc actgaagggg ggcagaactt tcagatcccc cctcttcccg
      721 aaactgtggc agcctggttt cctgaagcag atcgtcggga ggtttctaca caatggactt
      781 ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DADLCQ010000360          808 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000360 DADLCQ010000000
VERSION     DADLCQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.363"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>808)
                     /locus_tag="JQG95_004383"
     CDS             complement(<1..>808)
                     /locus_tag="JQG95_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY5890684.1"
                     /translation="EGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTK
                     EILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGEL
                     PSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQ
                     PGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSN
                     SFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNV"
BASE COUNT          181 a          209 c          219 g          199 t
ORIGIN      
        1 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcaa
       61 gctgaggaac ggcaactttg atcccggcgt tctgaaaact gtactgacca ttacgggtat
      121 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactggtacc cgggcttcat
      181 acagggtatc gtttaaacgt tgtagcgtta gccagtttgc cggatgcgaa agcagcgact
      241 gctgttccac gcgatcgcgt tccagcgtca ataacgcttc atcaattggc tccgggaaag
      301 taatcagcat cttcgcggtt tcgcctggct ggtacagcgt tttatccgcc acgatatcta
      361 ccgtaccagt atgcgccgtg ctgcccttac cgctgacggc atgacttaac ccagcgagaa
      421 ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc aggtttagcg aaattgacgg
      481 taaaggattt gccgcctgac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
      541 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
      601 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
      661 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
      721 cggttaacaa atagcggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
      781 tggcgtcgga caccgtttcg ctgccttc
//
LOCUS       DADLCQ010000361          806 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000361 DADLCQ010000000
VERSION     DADLCQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.364"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..806
                     /locus_tag="JQG95_004384"
                     /pseudo
     CDS             1..806
                     /locus_tag="JQG95_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          189 a          203 c          244 g          170 t
ORIGIN      
        1 gccggagaaa cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt
       61 tgaaccgggg atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt
      121 atttctctgg cgtaagcaat accaggaagg aagtcttact gctgtggccg ccggagaaca
      181 ggttgttcct gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct
      241 gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc
      301 aaaaaagtgg atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc
      361 cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg
      421 atggatggcc gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat
      481 gttatcggag agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag
      541 gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg tttaccggct catgcgccag
      601 aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc
      661 agagtggccg tgaaagaaag caatcagcga tggtgctctg acgggttcga gttctgctgt
      721 gataacggag agagactgcg tgtcacgttc gcgctggact gctgtgatcg tgaggcactg
      781 cactgggcgg tgactaccgg cggctt
//
LOCUS       DADLCQ010000362          799 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000362 DADLCQ010000000
VERSION     DADLCQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.365"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>799
                     /locus_tag="JQG95_004385"
     CDS             <1..>799
                     /locus_tag="JQG95_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HAY5890685.1"
                     /translation="LAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIM
                     LPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFW
                     RTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQ
                     LIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLV
                     KLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT          196 a          230 c          226 g          147 t
ORIGIN      
        1 ccttgccgaa tcaggtactg taacgctacg caagcgccgt cttcccctcg aaatgatggt
       61 ctggtgtatt gttggcatgg cgcttgagcg taaagaacct cttcaccaga ttgtgaatcg
      121 cctggacatc atgctgccgg gcaatcgccc cttcgttgcc cccagtgccg ttattcaggc
      181 ccgccagcgc ctgggaagtg aggctgtccg ccgcgtgttc acgaaaacag cgcagctctg
      241 gcataacgcc acgccgcatc cgcactggtg cggcctgacc ctgctggcca tcgatggtgt
      301 gttctggcgc acaccggata caccagagaa cgatgcagcc ttcccccgcc agacacatgc
      361 cgggaacccg gcgctctacc cgcaggtcaa aatggtctgc cagatggaac tgaccagcca
      421 tctgctgacg gctgcagcct tcggcacgat gaagaacagc gaaaatgagc ttgctgagca
      481 acttatagaa caaaccggcg ataacactct gacgttaatg gataaaggtt attactcact
      541 gggactgtta aatggctgga gcctggcggg agaacaccgc cactggatga tacctctcag
      601 aaagggagcg caatatgaag agctcagaaa actgggtaaa ggcgatcatc tggtgaagct
      661 gaaaaccagc ccgcaggcac gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg
      721 cctgctgacc gtgacgcgca aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc
      781 catgcgcttc cccggagga
//
LOCUS       DADLCQ010000363          788 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000363 DADLCQ010000000
VERSION     DADLCQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.366"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..118)
                     /locus_tag="JQG95_004386"
     CDS             complement(<1..118)
                     /locus_tag="JQG95_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY5890686.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLS"
BASE COUNT          153 a          245 c          193 g          197 t
ORIGIN      
        1 atgaaagcgt gatccgcgcc gcttcagcca gccattctgg ttgtgttatc agacgcagca
       61 gccgttgaat ccgtagggac ggatcggtgg ctgcccatgt ggcaagcttg cgttgcattt
      121 cgctgattat caaaggtctt cacctcgtta ggccagttaa ttcacgtcgc aaacacattc
      181 aaactgcttc ccttcgccat gtaatgggct ttccccatcg cggactacta cggaagctcc
      241 gccagccagc gcgtcatcgg agccatgccc ccttaacatc cgtcgctgac cttccccggt
      301 ttacctgcct ggactcaggc atactgagga ggctgcccgt cgcactcttt atccttgctt
      361 gccgcaagtt ggcagaagtc agcaacgcaa gcgtgataga cgctgctgcc ccggtgtttc
      421 gcatacatgt caaaacacct tcgaccggca gtgcttacgt atcactgcca gttcctcctg
      481 cacggcctgt cagatcacgt aggccgtggt gacgttttca acccacagag gcggattaac
      541 gggttcatgt tcttcagcct ttcagtactt aaccttgagg atcatctcgg cttagtgatc
      601 tcgcctcaat ccccgttgtc agcgggttac atcaccctgc gggcatgccg caggtcactg
      661 ccgctcaggt tctccaccgt cacacccggt gggattgttg ggtttctcat cgtgagttac
      721 cggttcaata ttccagacag actcgcggtt catttaagca tccatgcccg ccctgaactc
      781 cgggcaca
//
LOCUS       DADLCQ010000364          782 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000364 DADLCQ010000000
VERSION     DADLCQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.367"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..321
                     /locus_tag="JQG95_004387"
     CDS             <1..321
                     /locus_tag="JQG95_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890687.1"
                     /translation="RSGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRL
                     SPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSL
                     SEIR"
     gene            325..782
                     /locus_tag="JQG95_004388"
                     /pseudo
     CDS             325..782
                     /locus_tag="JQG95_004388"
                     /inference="COORDINATES: protein motif:HMM:NF026233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
BASE COUNT          245 a          168 c          163 g          206 t
ORIGIN      
        1 cgctcaggtg taaacatcta tgcgtggatg ccggtattaa gctgggattt agatcccaca
       61 ttaacgcgag taaaatactt accaacaggg gagaaaaaag cacaaattca tcctgaacaa
      121 tatcaccgtc tctctccttt cgatgacaga gtcagagcac aagttggcat gttatatgaa
      181 gatcttgccg gacatgctgc ttttgatggc atattgttcc acgatgatgc tttgctttca
      241 gattatgaag atgccagtgc accggctatc acggcttatc agcaagcagg ctttagcggg
      301 agtctgagcg aaattcgata aaacccggag caatttaaac agtgggcccg ctttaaaagt
      361 cgtgcgttaa ctgacttcac tttagaactt agtgcgcgcg taaaagccat tcgcggtcca
      421 catattaaaa ctgcacgaaa tatttttgca cttccggtaa tacaacctga aagtgaagcc
      481 tggtttgcac agaattatgc tgatttccta aaaagctatg actggaccgc tattatggct
      541 atgccttata tggaaggtgt cgcagaaaaa tcggctgacc aatggttaat acaattgacc
      601 aatcaaatta aaaacatccc tcaggctaaa gacaaatcta ttttagaatt acaggcacaa
      661 aactggcaga aaaatggtca gcatcaggct attccttcgc aacaactcgc tcactggatg
      721 agcctattac aactgaatgg agtgaaaaac tatggttatt atcccgacaa ttttctgcat
      781 aa
//
LOCUS       DADLCQ010000365          772 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000365 DADLCQ010000000
VERSION     DADLCQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.368"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..582
                     /locus_tag="JQG95_004389"
     CDS             <1..582
                     /locus_tag="JQG95_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HAY5890688.1"
                     /translation="WSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARA
                     INELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRL
                     RKLFQNTWRAVVEGMDYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQP
                     DGTRSPNLADSVMISYAPMNSALNIWELLGRQA"
     gene            582..>772
                     /locus_tag="JQG95_004390"
     CDS             582..>772
                     /locus_tag="JQG95_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890689.1"
                     /translation="MARNKQATRRTAQATADGYENFVARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEA"
BASE COUNT          185 a          210 c          218 g          159 t
ORIGIN      
        1 tggtccggtg tgggcagcga catttatcag tccgtcgaga aggttttcgg cttttgcgaa
       61 caggacaacc tcgaagagtt tcgctttgac gaggacgggt tgggcgctgg cgttcgcggc
      121 gatgcacgcg ctatcaacga actgcgtaac gctgcgcgcc gaccgtcaat actcgccaca
      181 ccgtttcgag gtagtggcgc ggtatttgat ccggacgacg aagcggtgcg cggtgacaac
      241 ggacaggccg cccgcctgaa caaggacttc ttcgctaacg ccaaagccca gagctggtgg
      301 cggttacgta aactttttca gaatacctgg cgcgccgtgg ttgaaggtat ggattacaac
      361 ccggacgaaa tcatctcaat cagcagtagc atggcactca aagataaact catcatcgag
      421 ctttcgcagc cgacctattc cattaatggt gtgggaaaaa tcgttattga taaacagcct
      481 gatggaaccc ggtcgccaaa ccttgccgac tcggtgatga tcagctacgc gccaatgaat
      541 tcagccctga acatctggga gctgctaggg agacaggcct gatggcacga aacaaacaag
      601 ccacgcggcg aacggcacag gccaccgctg atggctatga gaactttgtt gcccgcgtgg
      661 ggatgcagac acctaaccag cactcagcat ccacctaccg ggctaatttc accagtcgta
      721 accgcatgct ggtggaatgg tcctatcgtt cgtcctggat tatcggcgaa gc
//
LOCUS       DADLCQ010000366          761 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000366 DADLCQ010000000
VERSION     DADLCQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.369"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            28..300
                     /locus_tag="JQG95_004391"
     CDS             28..300
                     /locus_tag="JQG95_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY5890690.1"
                     /translation="MVRKFDELEAKCAAQENKVIPVSTELPPANESVLLFDANGEGWL
                     IGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            300..605
                     /locus_tag="JQG95_004392"
     CDS             300..605
                     /locus_tag="JQG95_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890691.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            521..>761
                     /locus_tag="JQG95_004393"
     CDS             521..>761
                     /locus_tag="JQG95_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890692.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQAC"
BASE COUNT          247 a          166 c          182 g          166 t
ORIGIN      
        1 aaagtaaatg aaacagatgc tgaatacctg gtgcgtaaat tcgatgagct tgaagctaaa
       61 tgtgcagcac aggaaaacaa agtaatacca gtgtcaactg aactgccacc agcaaatgaa
      121 agtgttttgt tattcgatgc taacggagaa ggctggctaa ttggctggcg ttctctctgg
      181 tacacctggg gacaaaaaga aaccggagaa tggcagtgga catttcaggt cggggacctt
      241 gaaaacgtca atatcactca ctgggcagta atgccaaaag caccggaggc tggagcataa
      301 tgaccacttt taccgacaaa gaactgatta aagaaattaa agagcgtatc agcagccttg
      361 acgtgcgaga cgatattgag cgccgtgctt atgaaatcgc actcctatct ctggaagtag
      421 aaccagatga acgcgaagct tatgaattat tcatggaaaa gcgttttggt gacttagtag
      481 atcgtcggag agcaaaaaac ggcgataacg aatacatggc atgggatatg actctcggtt
      541 ggatcgtctg gcagcaacga gctggtatcc atttctcaac aatgtcacag caagaggtga
      601 aataatggag ccatacagcc tcacactcga tgaggcctgt cattttctca agatatccag
      661 accgactgcc attaactgga tacgcacagg gcgtcttcag gcaacacgca aagatcccac
      721 taagaataaa tctccttacc tcacaacacg acaagcctgc a
//
LOCUS       DADLCQ010000367          758 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000367 DADLCQ010000000
VERSION     DADLCQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.370"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            202..>758
                     /locus_tag="JQG95_004394"
     CDS             202..>758
                     /locus_tag="JQG95_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890693.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          263 a          146 c          140 g          209 t
ORIGIN      
        1 acatcacccc ccggatcatg gacgactggg ggcagtaatc atgccgggtc tggctggcta
       61 acaccccatt ataaggggta cgaataaagt aacgggggaa gcaatacctg gagaaagagt
      121 acaaagcaaa ctgtattaaa cttaataagt tatattgtat ttcactagag ttcttatcgt
      181 tgataagaaa ttctggttat aatgattaaa tcaacgaata tacaggtaat cggttctggt
      241 attatgcatc aaataaacaa catacactcg ttaactctat tttctttgcc tgtgagtttg
      301 agtccatctt gtaatgaata ttatttaaaa gtctggagtg aatgggaaaa gaacggtacc
      361 ccaggcgagc aacgcaatat cgccttcaat aggctaaaaa tatgtttgca aaatcaaggg
      421 gcggaattaa atctatctga gttagattta aaaacattac ctgatttacc gcctcagata
      481 acaacactgg aaataagaaa aaacctatta acatatctcc ctgatttccc accaatgctt
      541 aaggtaatac atgctcaatt taatcaactg gaaagcttac ctgccttacc cgagacgtta
      601 gaagagctta atgtgggtga taacaagata aaagaattac catatcttcc tgaaactcta
      661 actcatttac ggatccataa taaccgattg catattctgc cactattgcc accggagcta
      721 aaattactga tagtttctgg aaacagatta gacagcat
//
LOCUS       DADLCQ010000368          758 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000368 DADLCQ010000000
VERSION     DADLCQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.371"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>758
                     /locus_tag="JQG95_004395"
     CDS             <1..>758
                     /locus_tag="JQG95_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890694.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          183 g          209 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagca
//
LOCUS       DADLCQ010000369          750 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000369 DADLCQ010000000
VERSION     DADLCQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.372"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>750
                     /locus_tag="JQG95_004396"
     CDS             <1..>750
                     /locus_tag="JQG95_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890695.1"
                     /translation="VHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGV
                     PQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKP
                     AVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFL
                     GHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGW
                     AMFYQFVDFKAKVFSYIDRVVFWKLAHWLAR"
BASE COUNT          197 a          179 c          212 g          162 t
ORIGIN      
        1 ccgtacatca tcgactgctg atgaaggctg tacgccgcag gatcagtgac gcacgtttca
       61 tgactctgct gtggaaaacc atcaaggcgg gacatatcga tgtcggtctc tttcgggcgg
      121 ccagtgaagg tgtgccacag ggcggtgtta tatcgccgct attatcgaac atcatgctga
      181 atgagttcga tcaatacctg catgagcgct acctgagcgg gaaagccaga aaagatcggt
      241 ggtactggaa taacagtatc caacggggcc gaagtacggc ggtcagagaa aactggcagt
      301 ggaaacccgc ggtggcgtac tgccgctatg ccgatgattt tgtcctcatc gtcaaaggca
      361 ccaaagcaca ggcggaagcc atcagggagg agtgtcgggg tgtgctcgaa ggcagtctga
      421 aactcaggct gaacatggat aagactaaaa tcacccatgt taatgacggc tttatctttc
      481 tggggcacag gatcattcgc aaacgcagtc gttatggcga gatgcgagtg gtctcaacga
      541 tcccgcagga gaaagccaga aacttcgccg catcgctgac agcactgtta tcaggcaact
      601 acagtgaaag caaagtcgat atggctgaac aactcaaccg aaaactgaaa ggctgggcca
      661 tgttctatca gttcgttgat tttaaggcca aagtcttcag ttatatcgac cgtgtcgtgt
      721 tctggaagct ggctcactgg ctggcccgca
//
LOCUS       DADLCQ010000370          745 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000370 DADLCQ010000000
VERSION     DADLCQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.373"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..120
                     /locus_tag="JQG95_004397"
     CDS             <1..120
                     /locus_tag="JQG95_004397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAY5890696.1"
                     /translation="ERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            117..>745
                     /locus_tag="JQG95_004398"
     CDS             117..>745
                     /locus_tag="JQG95_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HAY5890697.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQL"
BASE COUNT          172 a          205 c          222 g          146 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt
      421 tcagcccggt cagctaccgc gcacacggcc tacctgtgtc agaaaatctg ttggagcagg
      481 atttttacgc cagtggcccg aaccagaagt ggccaggaga catcacgtac ttacgtacag
      541 atgaaggctg gctgtatctg gcagtggtca ttgacctgtg gtcacgtgcc gttattggct
      601 ggtcaatgtc gccacgcatg acggcgcaac tgccctgcga tgccctgcag atggcgctgt
      661 ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc cagtactgtt
      721 cagcagatta tcaggcgcaa ctgaa
//
LOCUS       DADLCQ010000371          699 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000371 DADLCQ010000000
VERSION     DADLCQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.374"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>699
                     /locus_tag="JQG95_004399"
     CDS             <1..>699
                     /locus_tag="JQG95_004399"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5890698.1"
                     /translation="AMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELRE
                     IPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGID
                     IDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEAL
                     PQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYL
                     ADEIAKLGPYEFICT"
BASE COUNT          146 a          193 c          202 g          158 t
ORIGIN      
        1 ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc
       61 aaacctggtg tgcggtccgg tgcaaatctg ctggcataaa ttcgcccgct actgggatgt
      121 ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga aacgcatgat
      181 tgaagcctgc gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg
      241 taactatgag ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg
      301 tatcgacatc gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc
      361 cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca
      421 taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct
      481 gccgcaggaa ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat
      541 caacttctcc cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg
      601 tcgtgaaggc tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga
      661 tgagatcgcc aaactggggc cgtatgagtt catctgtac
//
LOCUS       DADLCQ010000372          692 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000372 DADLCQ010000000
VERSION     DADLCQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.375"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            36..>692
                     /locus_tag="JQG95_004400"
     CDS             36..>692
                     /locus_tag="JQG95_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY5890699.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccggac gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 gtaatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DADLCQ010000373          687 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000373 DADLCQ010000000
VERSION     DADLCQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.376"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..667)
                     /locus_tag="JQG95_004401"
     CDS             complement(<1..667)
                     /locus_tag="JQG95_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY5890700.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          185 a          173 c          154 g          175 t
ORIGIN      
        1 ttgccgcatc atcatcagac tccggaacgc cctcatacac atgtttaaaa ccatccggat
       61 cacggcgtcg cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt
      121 catagttaat tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga
      181 cgaaatcagt aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga
      241 tcgttggctc cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccagccaca
      301 acatatcgat cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa
      361 caaattccga gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc
      421 gcagaccaaa cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat
      481 tctggatctg acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa
      541 tgccagcggc atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg
      601 cccgcatagt ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag
      661 aagtcattga ggtcacgctc tccattg
//
LOCUS       DADLCQ010000374          681 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000374 DADLCQ010000000
VERSION     DADLCQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.377"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            58..>681
                     /locus_tag="JQG95_004402"
     CDS             58..>681
                     /locus_tag="JQG95_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY5890701.1"
                     /translation="MNDFARIAGGQNSRSNILSPRAFLEFLAKIFTLGYVDFSKRSNE
                     AGRNMMAHIEFSSYSKDTDGNEKMKFYMNNPEGERADLSKVKIEITLASASTKGIREG
                     HTVIIFKQSDGSTNRYEGKSFERKDDSSLHLITNKVLACYQREANKKIARLLNNHQKL
                     NNIQELNDSQELNNSQKLNNSQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT          238 a           99 c          147 g          197 t
ORIGIN      
        1 acattagccg acaattttaa acaaaaaaat gaagccttag atacctggta tgtgggaatg
       61 aatgattttg cccgaattgc cggggggcag aatagcagaa gcaatattct ttctcctaga
      121 gcatttttgg agtttttggc aaagatattt accctgggtt atgtggattt tagcaaacgc
      181 tccaacgaag cgggtagaaa tatgatggct catattgagt tctcatctta ttctaaagat
      241 actgatggca atgaaaaaat gaagttttac atgaataatc ctgaagggga gcgagcggat
      301 ttatctaagg tgaagataga aattacactt gcctcagctt ctactaaagg gattcgtgaa
      361 ggacatacag tcattatatt taaacaatct gatggttcga ctaatcgtta tgaagggaag
      421 tcttttgaaa gaaaagatga tagttcatta cacctgatta ctaacaaggt tctggcgtgt
      481 taccaacgcg aagctaacaa gaaaatagcg cgtctattaa ataatcatca gaagttaaat
      541 aatatacagg agttaaatga ttcgcaggag ttaaataatt cacagaagtt aaataattcg
      601 caggagttaa ataactcgca ggtgagttgt aaaggttcag ttgattctac gattacggat
      661 ttattagaaa aagcattgaa t
//
LOCUS       DADLCQ010000375          672 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000375 DADLCQ010000000
VERSION     DADLCQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.378"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT          211 a          165 c          130 g          166 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga
      421 gtgtgttccc ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca
      481 ccagaatgat gaatcatcag tcccctgcca gaggttcact acgtaacctg accgacaatg
      541 caccattctg gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag
      601 gagatggtaa ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc
      661 gtctcaaatc tc
//
LOCUS       DADLCQ010000376          672 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000376 DADLCQ010000000
VERSION     DADLCQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.379"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            155..340
                     /locus_tag="JQG95_004403"
                     /pseudo
     CDS             155..340
                     /locus_tag="JQG95_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..619)
                     /locus_tag="JQG95_004404"
     CDS             complement(440..619)
                     /locus_tag="JQG95_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY5890702.1"
                     /translation="MSRLKTYGYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAET
                     AAILYALLEKYFPGK"
BASE COUNT          194 a          167 c          157 g          154 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt ca
//
LOCUS       DADLCQ010000377          656 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000377 DADLCQ010000000
VERSION     DADLCQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.380"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(151..648)
                     /locus_tag="JQG95_004405"
     CDS             complement(151..648)
                     /locus_tag="JQG95_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY5890703.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          145 g          178 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DADLCQ010000378          636 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000378 DADLCQ010000000
VERSION     DADLCQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.381"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            88..153
                     /locus_tag="JQG95_004406"
                     /pseudo
     CDS             88..153
                     /locus_tag="JQG95_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JQG95_004407"
     CDS             381..629
                     /locus_tag="JQG95_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY5890704.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DADLCQ010000379          630 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000379 DADLCQ010000000
VERSION     DADLCQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.382"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..112)
                     /locus_tag="JQG95_004408"
     CDS             complement(<1..112)
                     /locus_tag="JQG95_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY5890705.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYI"
     gene            complement(109..399)
                     /locus_tag="JQG95_004409"
     CDS             complement(109..399)
                     /locus_tag="JQG95_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY5890706.1"
                     /translation="MVDLLKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(399..>630)
                     /locus_tag="JQG95_004410"
     CDS             complement(399..>630)
                     /locus_tag="JQG95_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY5890707.1"
                     /translation="FREWVAIQAGFYTEHFYPDGSRGCRAKSIAFANMDETEFQQVYK
                     SVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          149 a          181 c          136 g          164 t
ORIGIN      
        1 caatatacga ccagcgcccc cacatacgta gttttccctg aatccagaca ctttccagcg
       61 tggtgagacg aaggtgttct ccgctttttc ctgtattcgt tgggtaaatc ataaatatcc
      121 ctcctttctc cagatttctt gtgtgcgaaa aacaccttct gcatgcatca ggcgtaattc
      181 ttctttggtg taatcgctgg tttttacccg cccgtcgatt aaatcgtggc acgagctaca
      241 ggcaatcgct gcctgcatat cgtgtggctt tatcgctgtt ccgcacgttc ccgccagtcg
      301 gtaatgcgcc agcacagacg tttccggatc gtgattgcag tagccaggaa ttctgacggt
      361 gcacatctgc ccccgcgccg ctttaagtaa atccaccatt acgcaaactc cagcagctgc
      421 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacagaat ccagttccac
      481 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat attcgcaaac
      541 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
      601 ccggcctgaa tggctaccca ctcgcggaaa
//
LOCUS       DADLCQ010000380          616 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000380 DADLCQ010000000
VERSION     DADLCQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.383"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            456..>616
                     /locus_tag="JQG95_004411"
     CDS             456..>616
                     /locus_tag="JQG95_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890708.1"
                     /translation="MSQQPQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLR
                     DIRKMLEEL"
BASE COUNT          179 a          148 c          122 g          167 t
ORIGIN      
        1 agattaactt taagtattaa ataaaagatt taattaaaga ccaattaaag attaactcta
       61 agggaatacc ttaaggggtg atgatattta taataatcaa tcaccaaacc tataggtctt
      121 ctctagggtc tctctatagg tctctctagg gtctcctcta ggcattaccc ccagaagcta
      181 actctatggc cctctatggt cctctatggc cctgtacgtc attctttagg atgccctgta
      241 gtttcgtata gcctgatgcc tagagagacc ctagagtggc tcctagagag accctcccag
      301 tagctccgca cagctacatc gtcatcatca tagtattcaa tctcaaagtc attctgtttg
      361 catcctgttg tcctgctggt ctcccttcgc ttggggactt tgagtaggtc agtgggggtg
      421 cgacttcagg gtgcaaacaa aaaggaaatc aagcgatgtc tcaacaacca caacaaccac
      481 aacaaccaca acaagaaatc agcatcatta acctcgacca gcttgtgtcc atgacctcag
      541 ttgaaattgc tgagctgaca ggcaaggagc accgaaatgt ccttcgtgat attcgtaaga
      601 tgctcgaaga acttaa
//
LOCUS       DADLCQ010000381          583 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000381 DADLCQ010000000
VERSION     DADLCQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.384"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..414)
                     /locus_tag="JQG95_004412"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="JQG95_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            337..>583
                     /locus_tag="JQG95_004413"
     CDS             337..>583
                     /locus_tag="JQG95_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890709.1"
                     /translation="MPGTTTAMSINFIGITARIMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          160 a          132 c          147 g          144 t
ORIGIN      
        1 aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc cagtgttgtt
       61 gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag
      121 cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg tccgtatgac
      181 ggtattgatg ccgattttca gtgttctggc ggtataacag ccccctgcgc cgttatgtgc
      241 catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt aagtgagctg
      301 gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg
      361 tcgataaact tcatcggaat tacagcgcgg ataatgaaca gtaacggtag tcatggaaaa
      421 cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac cttttgccgc
      481 aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg aaaaaaatta
      541 tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DADLCQ010000382          575 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000382 DADLCQ010000000
VERSION     DADLCQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.385"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..246
                     /locus_tag="JQG95_004414"
     CDS             <1..246
                     /locus_tag="JQG95_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY5890710.1"
                     /translation="NRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVL
                     TVGLVLCAGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            301..>575
                     /gene="finO"
                     /locus_tag="JQG95_004415"
     CDS             301..>575
                     /gene="finO"
                     /locus_tag="JQG95_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY5890711.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDT"
BASE COUNT          128 a          149 c          185 g          113 t
ORIGIN      
        1 aaccggctgt accgggcgga agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg
       61 gctgttattc cggcgcttaa ccttgccacc agtgatgcag cggcggtggc cggtcttgtg
      121 atgacggtgc tgaccgttgg tctggtgttg tgtgccggaa aatcattacc ccgtttctgg
      181 cccggagact ttttcccgac gttctatgca tgtcatctgg ctgtgctggg cgttctggcg
      241 ctgtgacggg tgtggtatct ttggccgcaa gaggatgatt cgtcagaggc agaacacagc
      301 atgacagagc agaagcgacc ggtactgaca ctgaagcgga agacagaggg aacagcgcct
      361 gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaagtggaa ggtgaaaaag
      421 cagaagctgg ccgagaaagc ggcccgtgaa gcagagctgg cggcaaaaaa agcgcaggcc
      481 agacaggcgc tgtccattta tctgaacctg cccacgctgg atgaggctgt gaataccctg
      541 aagccctggt ggccgggatt atttgacggt gacac
//
LOCUS       DADLCQ010000383          575 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000383 DADLCQ010000000
VERSION     DADLCQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.386"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>575
                     /locus_tag="JQG95_004416"
     CDS             <1..>575
                     /locus_tag="JQG95_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY5890712.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEG"
BASE COUNT          131 a          142 c          169 g          133 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggcatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaagg
//
LOCUS       DADLCQ010000384          566 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000384 DADLCQ010000000
VERSION     DADLCQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.387"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..297
                     /locus_tag="JQG95_004417"
     CDS             <1..297
                     /locus_tag="JQG95_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY5890713.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JQG95_004418"
     CDS             complement(405..>566)
                     /locus_tag="JQG95_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890714.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLCQ010000385          558 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000385 DADLCQ010000000
VERSION     DADLCQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.388"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>558
                     /locus_tag="JQG95_004419"
     CDS             <1..>558
                     /locus_tag="JQG95_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY5890715.1"
                     /translation="LAVGHLQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSC
                     GYDAEYEQTEPCKAEQVDITGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAA
                     IKTGDADTMNELVESAPASVTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDS
                     DDDLKTLLKALLAKLERNATGDNDN"
BASE COUNT          158 a          139 c          164 g           97 t
ORIGIN      
        1 gcttgctgtc gggcacctcc agaatgtccg gcgcgggacg ggtgagcagt ctgatttgat
       61 gctggctgac cttatcgtca aagacgaaag cgccattcag cttatcgaag atggcctgcg
      121 cgaagtgtcg tgcggctatg acgcggagta cgagcagacc gagccatgta aagccgagca
      181 ggtcgatatt accggaaacc atgtggctct tgtccctaaa ggcagagccg gaaatcgttg
      241 tgcaattgga gacagagaca caatggcaaa tcaaaagaaa aactggtgga accgcatgcg
      301 tgcggccatc aagacaggag atgccgacac catgaacgaa ctggtggagt cggctcccgc
      361 atcggttaca ggagatgagg gggatttgcc gcagggcgtt aatctcaaca tcaacctgtc
      421 cccgcagcaa ccactaccgg acaaagcacc agagatgggc ggagatccaa ccggcgacag
      481 tgatgatgac ctcaaaacat tactgaaagc cctgctggct aagctggaaa gaaatgccac
      541 gggcgataac gataataa
//
LOCUS       DADLCQ010000386          551 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000386 DADLCQ010000000
VERSION     DADLCQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.389"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..551
                     /locus_tag="JQG95_004420"
                     /pseudo
     CDS             1..551
                     /locus_tag="JQG95_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          148 a          131 c          152 g          120 t
ORIGIN      
        1 ccgaacgata tggggcatgg aaaactgttt ataaccgctt taaccgatgg tcaaagtcag
       61 gcgtgattaa tattattttc aacaggttgc tttcgctact tgatgcaaac ggctttattg
      121 actggtctgc caccgcgctg gatggcagta atatccgggc gctgaaatgt gccgccggtg
      181 ctcaaaaaaa catcccgata tcgacggaga taatgggccg ggtcgctctc gcggcggttt
      241 tggcaccaaa atccatctgg caacagacgg aagtggcctc ccgttaaata tcgtgctaag
      301 ccccggacaa gctcatgaaa gccagttcgc gcaacgactt ctggacggga ttggcgttca
      361 gcgtcagaac ggcagcatga aacgccgtgg acatgcggta ctggctgata aagcgtattc
      421 agggcgcgca ttgcgcaacg agctgaaaaa taacggtata aaggcagtaa tcccccgaaa
      481 gtcaaatgag aaaatggcat cggatggacg tgcacagctt gatcgtgatg cgtactgcaa
      541 ccgtaatgtc g
//
LOCUS       DADLCQ010000387          537 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000387 DADLCQ010000000
VERSION     DADLCQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.390"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..130)
                     /gene="csgC"
                     /locus_tag="JQG95_004421"
     CDS             complement(<1..130)
                     /gene="csgC"
                     /locus_tag="JQG95_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY5890716.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQI"
     gene            complement(189..>537)
                     /gene="csgA"
                     /locus_tag="JQG95_004422"
     CDS             complement(189..>537)
                     /gene="csgA"
                     /locus_tag="JQG95_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY5890717.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          143 a          123 c          127 g          144 t
ORIGIN      
        1 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
       61 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
      121 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
      181 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
      241 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
      301 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
      361 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
      421 ccgccatgct gggtaatagt caagtcagag ttacgggcat cagtttgcag agcaagtgca
      481 gagttaccgc caccgtactg gtaaatgttc agctctgaat ttgggccgct attatta
//
LOCUS       DADLCQ010000388          537 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000388 DADLCQ010000000
VERSION     DADLCQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.391"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>537
                     /locus_tag="JQG95_004423"
     CDS             <1..>537
                     /locus_tag="JQG95_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890718.1"
                     /translation="QEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICEN
                     VVRTINARMVDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSEVSDYG
                     IIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGC
                     FRHDGENITVVLSRDGDSQ"
BASE COUNT          120 a          141 c          144 g          132 t
ORIGIN      
        1 caggagattg tcgtcgaacc gattgaacag gctattctca tctctgagca gactaagctg
       61 tgggacgcca agtcatccac cggattcact cagggcacac ttcagcagga cgctatgaac
      121 atctgtgaga atgtggtccg taccatcaac gctcgcatgg tcgacgttct ggaatcctct
      181 aagctgctga agaccgtaga gcttcctgtc ctgactggta gcctgaccgc taaggcagac
      241 gctatcatgg acgctctgta tgagaacacc gagccttctt tcggttctga ggtgtctgac
      301 tacggtatta tcgcccatga gagccaactg aaagcactgt cccgtctggc tgctaaacag
      361 ggcttctctg gtgaagacgc tatcgtggat atgctgggta ccgacatcgc gtattacaac
      421 ggtgaggaca aaggtgtgtt catgttggct aaacgcttca cggccctgag cttcggctgc
      481 ttccgtcacg acggtgagaa tattacggtc gtcctttcac gtgatggcga ctctcag
//
LOCUS       DADLCQ010000389          529 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000389 DADLCQ010000000
VERSION     DADLCQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.392"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            338..>529
                     /locus_tag="JQG95_004424"
     CDS             338..>529
                     /locus_tag="JQG95_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY5890719.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDL"
BASE COUNT          181 a          109 c          104 g          135 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcatat tggctacgta attaaaacag
       61 aaaataaaga aaccccattc actgtagttg atatcgacgg tctatcagga aactttaaaa
      121 ctcttgttga aggtgccaca aaaatgtgcc tagtttacat cggaaataat ctgcccgcag
      181 aaaaaaagcc gaatttctgg caaatctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      241 aaaagaaagc ctgcacagtg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      301 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg agcacaaaac cactcttcct
      361 gttacggaaa gcgaaaaaat catccggtga acctgacgtc gtcctgtggg caagcgacga
      421 ttttgaatcg accagcacca ctctggacta tctgctcgtt aagtcaggta aaaaactgag
      481 caactatttt aaagctgttg ccacaaattt tcctgtcgtt aatgacctt
//
LOCUS       DADLCQ010000390          523 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000390 DADLCQ010000000
VERSION     DADLCQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.393"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>523)
                     /locus_tag="JQG95_004425"
     CDS             complement(<1..>523)
                     /locus_tag="JQG95_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890720.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNVDNAMQ"
BASE COUNT          149 a          124 c          104 g          146 t
ORIGIN      
        1 tctgcatagc attatcaaca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc aca
//
LOCUS       DADLCQ010000391          519 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000391 DADLCQ010000000
VERSION     DADLCQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.394"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..221)
                     /locus_tag="JQG95_004426"
     CDS             complement(<1..221)
                     /locus_tag="JQG95_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890721.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTT"
     gene            complement(225..>519)
                     /locus_tag="JQG95_004427"
     CDS             complement(225..>519)
                     /locus_tag="JQG95_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890722.1"
                     /translation="EVIVPGIDLSRKMKPTAFKREVLASADKTLVRQIVGDADIRKLP
                     KQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIASLNKQNAEFWANRKG"
BASE COUNT          115 a          140 c          136 g          128 t
ORIGIN      
        1 gtggtcggat aagggcgcac gtagatcccc ttcaccttgt ctgcggtatc accatctgcc
       61 agcggcacga aaaaaccgtc atcatcgtat ttaccagcca ggccataggc agcgaaggcg
      121 ttatcggatt taaggaccac cggttcgacg gttaagtcct gcgggcgaga gacagccccg
      181 gcaatgccaa caggcatccg gtacagaaat acattattca ttttttaccc tttacggttt
      241 gcccagaatt cagcgttttg tttgttcagg gaagcgatac tggtcatgcc catgtttagg
      301 cgctgtgcat cgccggtggt ggcgcgggtg tttcgccctt tggcaacctc agacacggca
      361 ttaaacgcca tgtcgaccga ttgtttcggt aatttgcgga tatccgcatc accgactatc
      421 tggcgaacca gcgttttgtc agcagaagcc agaacctcgc gtttgaacgc ggtcggtttc
      481 atcttacggc tcagatcgat acccggaacg ataacttcg
//
LOCUS       DADLCQ010000392          494 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000392 DADLCQ010000000
VERSION     DADLCQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.395"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            28..>494
                     /locus_tag="JQG95_004428"
     CDS             28..>494
                     /locus_tag="JQG95_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY5890723.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQA"
BASE COUNT          138 a          102 c          143 g          111 t
ORIGIN      
        1 gataaaaatc acagagctgg agtgtgcatg aataaccagt atttacagtt tgttcgtgag
       61 cagctcatta tcgccaccgc tgatttgagt ggggcgacaa aaggtcagct tgaagcctgg
      121 caggagaatg ccatgttcga tacagggcgt tacaggcgta aaaaaatccg gtaccgcgat
      181 gaggtgaccg gaaaaatgat aacgcgggat aatccaccaa tcccgggaaa acaatcactg
      241 gcgaaggggg cgtcaattcc tctggtcagt caggttgcgt tttcgacatc atcgtggcgg
      301 cgggctgttc tgtctcttga agagcactat aaagcctggt tgttgtggtg ttacagcgga
      361 agtatttgct gggagtatca gatcaccata acccactggg cgtgggaaga gtttaaggct
      421 cattctggca ctaggaaaat tgcagagaaa acacaggaac gcctgaaaaa attaatctgg
      481 ctggcggcgc aggc
//
LOCUS       DADLCQ010000393          485 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000393 DADLCQ010000000
VERSION     DADLCQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.396"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..248
                     /locus_tag="JQG95_004429"
     CDS             <1..248
                     /locus_tag="JQG95_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY5890724.1"
                     /translation="YHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(245..>485)
                     /locus_tag="JQG95_004430"
     CDS             complement(245..>485)
                     /locus_tag="JQG95_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890725.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
BASE COUNT          121 a          123 c          137 g          104 t
ORIGIN      
        1 gctatcacca gaacggtgtt ccgctgaata tccgcgcggt taaatggctg aaaggccgtg
       61 gtgtggggaa taaggatcgc atggttgcgt acaccaacga taaaaaatac gtccgctacc
      121 cgctggttcc gcttcagagc gtgccggtgc agtatcgcgg tctgtatcag atcgtcactt
      181 actacggcaa gctgggtgca gtcgagccag tgtacaaaga aaccatttcg tacgttgatg
      241 gcatttaaca gccacatggc cccctggcgg ggccattaag gatgacccga tggcaaaaaa
      301 tgatgcagta atacacgtac ataccccgtt tgtgctcacg cttcccgacg gttctcggcg
      361 cgagtttgtt aaaggccgtc atgttgtgga ggaagacgtt gccacgcact ggttcactcg
      421 tgcgcacgcg gaagtatccg ttggcaaagc cacagacgcg cgtaacgagg taaaaaatgc
      481 caaag
//
LOCUS       DADLCQ010000394          484 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000394 DADLCQ010000000
VERSION     DADLCQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.397"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>484
                     /locus_tag="JQG95_004431"
     CDS             <1..>484
                     /locus_tag="JQG95_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY5890726.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          109 a          107 c          123 g          145 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaa
//
LOCUS       DADLCQ010000395          484 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000395 DADLCQ010000000
VERSION     DADLCQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.398"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(212..>484)
                     /locus_tag="JQG95_004432"
     CDS             complement(212..>484)
                     /locus_tag="JQG95_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY5890727.1"
                     /translation="RTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSMP
                     ELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
BASE COUNT          106 a          165 c          108 g          105 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttctctgttc
      121 agcctgaccg cacaccccgg cggccggatg acagactccg cctcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attacttaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagactgctc agctcctctt tcatctcccg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tccgcatcac tccacaccat atgtgtttgt gccgcgacag
      421 attttgccag catgaccacc ggggacaggc gggcaactga cgaggcgtca gcgttccacg
      481 tgcg
//
LOCUS       DADLCQ010000396          480 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000396 DADLCQ010000000
VERSION     DADLCQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.399"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(34..>480)
                     /locus_tag="JQG95_004433"
     CDS             complement(34..>480)
                     /locus_tag="JQG95_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY5890728.1"
                     /translation="EGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQDNAQETKEL
                     AGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHATREQRKHIT
                     ALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAEVYWQ"
BASE COUNT           99 a          122 c          124 g          135 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc cgatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccggtt ttagttccca
      421 tgtcatggag tctttgctga gttgatagcg ttcactccag gtaaaatcga tctcaccttc
//
LOCUS       DADLCQ010000397          479 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000397 DADLCQ010000000
VERSION     DADLCQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.400"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..250)
                     /locus_tag="JQG95_004434"
     CDS             complement(<1..250)
                     /locus_tag="JQG95_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890729.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          135 a           98 c           89 g          157 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta
      421 gtgcgggtgc gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DADLCQ010000398          471 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000398 DADLCQ010000000
VERSION     DADLCQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.401"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..471
                     /locus_tag="JQG95_004435"
                     /pseudo
     CDS             1..471
                     /locus_tag="JQG95_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          117 a          121 c          133 g          100 t
ORIGIN      
        1 aacgggttac cgacgaacgg gacatattaa aaaaagccgc ggcgtacttc gcaaagctgt
       61 ccgactgagg tacgccttta tccgtgacaa ctcctgttgc tggcctgttc gcctgctctg
      121 tcgggtgctg gatgttcatc ccagtggttt ttacgcctgg cttcagcagc cgcattcaca
      181 acgccatcag gcagacctga gactgacagg acagattaaa cagttctggc tggaatcggg
      241 atgcgtctat ggttatcgca aaatccatct ggatctgcgt gacagcgggc aacagtgcgg
      301 agtaaacaga gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg
      361 aagcccgcgg gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca
      421 gttcaatccg gatgctcctg atgagcgttg ggtaacggac ataacctaca t
//
LOCUS       DADLCQ010000399          455 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000399 DADLCQ010000000
VERSION     DADLCQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.402"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>455)
                     /locus_tag="JQG95_004436"
     CDS             complement(<1..>455)
                     /locus_tag="JQG95_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY5890730.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          108 a          125 c          124 g           98 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg cccctgtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacc
//
LOCUS       DADLCQ010000400          453 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000400 DADLCQ010000000
VERSION     DADLCQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.403"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..379)
                     /locus_tag="JQG95_004437"
     CDS             complement(<1..379)
                     /locus_tag="JQG95_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890731.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSL"
BASE COUNT          137 a          106 c           98 g          112 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcggtatga agt
//
LOCUS       DADLCQ010000401          448 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000401 DADLCQ010000000
VERSION     DADLCQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.404"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(15..230)
                     /locus_tag="JQG95_004438"
     CDS             complement(15..230)
                     /locus_tag="JQG95_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY5890732.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          145 a          107 c           95 g          101 t
ORIGIN      
        1 taatgatggc gacattattc acctctcgca gccttacgct tgtcttctct gattttgaag
       61 tacagatttg tcagataagt caggaagccc agaaccagac tccccagtac accaatcgca
      121 gcccactgtg acggactgac ctgatcaagc cactgtaaaa accagtagcc agcactgcct
      181 gcggaggtgc cgtaggcaat gcccgttgaa attttgtcca tggatttcat agcctcacct
      241 ccgcaaataa cggatggcgt agttttacac tgagaaatga aagggatttg aaaagaaaaa
      301 accgcaaaag cgggcgaaac gatatataca gtaaggaaag cactctatcc aacaaaccac
      361 tcacagttaa tcggaataaa agcagagtgc ttatgaatga tcgcctgccc gaaggttagt
      421 atttctgcac agcaattttg caaaaaaa
//
LOCUS       DADLCQ010000402          445 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000402 DADLCQ010000000
VERSION     DADLCQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.405"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..445)
                     /locus_tag="JQG95_004439"
     CDS             complement(<1..445)
                     /locus_tag="JQG95_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY5890733.1"
                     /translation="MLSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          101 a          113 c           86 g          145 t
ORIGIN      
        1 catgttcgaa caccactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
       61 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
      121 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
      181 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
      241 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
      301 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
      361 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
      421 atgatgctac ccggttgctt agaat
//
LOCUS       DADLCQ010000403          439 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000403 DADLCQ010000000
VERSION     DADLCQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.406"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..202)
                     /locus_tag="JQG95_004440"
     CDS             complement(<1..202)
                     /locus_tag="JQG95_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY5890734.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
     gene            302..>439
                     /locus_tag="JQG95_004441"
     CDS             302..>439
                     /locus_tag="JQG95_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY5890735.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          116 a          104 c           92 g          127 t
ORIGIN      
        1 gagaataacg tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt
       61 tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct
      121 gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca
      181 aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac
      241 gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact
      301 aatgcggatg aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt
      361 tcgtgcagca ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac
      421 actggaacaa cgtcttaaa
//
LOCUS       DADLCQ010000404          434 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000404 DADLCQ010000000
VERSION     DADLCQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.407"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            35..434
                     /locus_tag="JQG95_004442"
                     /pseudo
     CDS             35..434
                     /locus_tag="JQG95_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DADLCQ010000405          427 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000405 DADLCQ010000000
VERSION     DADLCQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.408"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(1..279)
                     /locus_tag="JQG95_004443"
     CDS             complement(1..279)
                     /locus_tag="JQG95_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890736.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a           99 c          134 g           99 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggat
//
LOCUS       DADLCQ010000406          418 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000406 DADLCQ010000000
VERSION     DADLCQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.409"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..373
                     /locus_tag="JQG95_004444"
     CDS             <1..373
                     /locus_tag="JQG95_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY5890737.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a          100 c          105 g           92 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtggatc
//
LOCUS       DADLCQ010000407          417 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000407 DADLCQ010000000
VERSION     DADLCQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.410"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(196..>417)
                     /locus_tag="JQG95_004445"
     CDS             complement(196..>417)
                     /locus_tag="JQG95_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY5890738.1"
                     /translation="VKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHR
                     LDSEALLFVMRTRSEQKAAFSKQSIAKVD"
BASE COUNT          117 a          103 c           72 g          125 t
ORIGIN      
        1 accactgagc taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact
       61 catctgacca ccagcacgtt taacgtcctg tgccattttt caggcataaa aaaaccgctc
      121 ggcggcgggt tttgcttact ttaccatcgc gtacaaaatc ggcaaagtat cagatttgca
      181 tgagatatat gccttttaat ctacttttgc aatactttgc tttgaaaatg ccgctttttg
      241 ttctgaacgt gttctcatca caaacaataa agcctcacta tccagtcggt gaaaaatgtg
      301 tttcattaca acccagtgac cagtaaatgt tttggaccag tttttggttg ttactcccgc
      361 cagtaatgcc agctcctgat attcataacc ttccccacca aaaagttctg cttttac
//
LOCUS       DADLCQ010000408          407 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000408 DADLCQ010000000
VERSION     DADLCQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.411"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>407
                     /locus_tag="JQG95_004446"
     CDS             <1..>407
                     /locus_tag="JQG95_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5890739.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLI"
BASE COUNT           85 a          144 c          102 g           76 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatc
//
LOCUS       DADLCQ010000409          395 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000409 DADLCQ010000000
VERSION     DADLCQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.412"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..177)
                     /locus_tag="JQG95_004447"
     CDS             complement(<1..177)
                     /locus_tag="JQG95_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY5890740.1"
                     /translation="MNKSLTVLPSGEYPTMSSLEMVDYINADRKSKAEAEGLAFPCKK
                     YRKLEHRSFMKKVPR"
BASE COUNT           95 a           94 c           95 g          111 t
ORIGIN      
        1 cctgggtact ttcttcatga aactgcggtg ttcgagtttg cgatatttct tacaggggaa
       61 cgcaagcccc tccgcctccg ctttggattt ccgatcagca ttaatgtaat ccaccatctc
      121 aagactgctc atggtcgggt attcgccaga aggaagaacg gttaatgatt tgttcatgtc
      181 ggtatttcct ttagaaagtt gagcctgttc gcacagaaaa gccgtccccg agaggtcctc
      241 acctatacgg cagttctcag gctcagcttt ctgaaagact cgggattgtt acgcgctgcg
      301 atgcgcggtt tactgcggac atataaaagc cccgcaagtg cgaggctcat taaatggact
      361 ttgtgatttg caaaaaaatt tatttcaggc actga
//
LOCUS       DADLCQ010000410          394 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000410 DADLCQ010000000
VERSION     DADLCQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.413"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..333
                     /locus_tag="JQG95_004448"
     CDS             <1..333
                     /locus_tag="JQG95_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890741.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIY
                     DSFCRGVA"
     gene            330..>394
                     /locus_tag="JQG95_004449"
     CDS             330..>394
                     /locus_tag="JQG95_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890742.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
BASE COUNT          100 a          114 c          104 g           76 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaatct ggtgagcttc gacaaccccc cccagcatca tccactctcc
      301 atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga
      361 tggcgctcgc cggacagttg caactggaaa gcct
//
LOCUS       DADLCQ010000411          375 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000411 DADLCQ010000000
VERSION     DADLCQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.414"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>375
                     /locus_tag="JQG95_004450"
     CDS             <1..>375
                     /locus_tag="JQG95_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5890743.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLCQ010000412          374 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000412 DADLCQ010000000
VERSION     DADLCQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.415"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>374
                     /locus_tag="JQG95_004451"
     CDS             <1..>374
                     /locus_tag="JQG95_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890744.1"
                     /translation="VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLE
                     PKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSAL
                     GYRSPREYLRQRACNGLSDNRCL"
BASE COUNT           96 a           86 c          116 g           76 t
ORIGIN      
        1 gtcatgctgg gagcggtgga acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag
       61 tggctgacgg ataatggttc atgctaccgg gctaatgaaa cacgccagtt cgcccggatg
      121 ttgggacttg aaccgaagaa cacggcggtg cggagtccgg agagtaacgg aatagcagag
      181 agcttcgtga aaacgataaa gcgtgactac atcagtatca tgcccaaacc agacgggtta
      241 acggcagcaa agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt
      301 gcgctgggtt atcgctcgcc acgggaatat ctgcggcagc gggcttgtaa tgggttaagt
      361 gataacagat gtct
//
LOCUS       DADLCQ010000413          372 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000413 DADLCQ010000000
VERSION     DADLCQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.416"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..311)
                     /locus_tag="JQG95_004452"
     CDS             complement(<1..311)
                     /locus_tag="JQG95_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5890745.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DADLCQ010000414          357 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000414 DADLCQ010000000
VERSION     DADLCQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.417"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>357
                     /locus_tag="JQG95_004453"
     CDS             <1..>357
                     /locus_tag="JQG95_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5890746.1"
                     /translation="ENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKR
                     YRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVIS
                     YSLSERPVMNMVENMLD"
BASE COUNT          107 a           77 c           89 g           84 t
ORIGIN      
        1 gagaatagag gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa
       61 cagattaacc ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt
      121 aaggtcaagc gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc
      181 caaagagatt tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt
      241 gcagtcaatg ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt
      301 atttcttaca gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgat
//
LOCUS       DADLCQ010000415          353 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000415 DADLCQ010000000
VERSION     DADLCQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.418"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            97..336
                     /locus_tag="JQG95_004454"
     CDS             97..336
                     /locus_tag="JQG95_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890747.1"
                     /translation="MLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAG
                     RNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          127 a           75 c           81 g           70 t
ORIGIN      
        1 tgaattattt gccaacgacg gtacaccgac atggctaaaa gaaaagcgtg caaaagaaat
       61 cgcacttgat gaaattaact tgatgaaatt atcgagttgc tcaacaagca ccatgggggc
      121 accagtaacg acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc
      181 tcatgggaaa gaatcaaagg tatggactgg ccgacaatca aagctggtcg taatgcacta
      241 tggattgaac tggaaggcgt tgaatatgca ttcccggatc cacaaacaca aaacgaaacg
      301 caacaagcag gatacgatga gaatattccg gtgtaaaacc aagtgcattt tgg
//
LOCUS       DADLCQ010000416          352 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000416 DADLCQ010000000
VERSION     DADLCQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.419"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(43..330)
                     /locus_tag="JQG95_004455"
     CDS             complement(43..330)
                     /locus_tag="JQG95_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890748.1"
                     /translation="MDEEYRKDLQLWFGLTHTSFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           94 a           88 c           70 g          100 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgta tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtctttacc tttgttgcaa tg
//
LOCUS       DADLCQ010000417          343 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000417 DADLCQ010000000
VERSION     DADLCQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.420"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           84 a           70 c           89 g          100 t
ORIGIN      
        1 ttgttagtct ttgcatgagc aagcggactg acacatttct ctttgttctc attcagaaaa
       61 tctcatcagt cggcgttcag accggtgttg tgctttatgg atacgttacg ctcctgatga
      121 cgtcatttga cgttcaacag catcacgggg ccgcacgaca tttcacgtca gttagtgcta
      181 tagctcagga acaaattttc ccaaattggg atatgctcgc aaattgctgg tgatgtgggg
      241 agaatctggt tgagttcggt agaattgatt tggaggcaga acgcttaaat cgtggcgtcc
      301 tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg aat
//
LOCUS       DADLCQ010000418          342 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000418 DADLCQ010000000
VERSION     DADLCQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.421"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>342)
                     /locus_tag="JQG95_004456"
     CDS             complement(<1..>342)
                     /locus_tag="JQG95_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890749.1"
                     /translation="QKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPE
                     DVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRY
                     RAKSLTFYGING"
BASE COUNT           76 a           97 c           89 g           80 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc tg
//
LOCUS       DADLCQ010000419          340 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000419 DADLCQ010000000
VERSION     DADLCQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.422"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..170
                     /gene="xisR"
                     /locus_tag="JQG95_004457"
     CDS             <1..170
                     /gene="xisR"
                     /locus_tag="JQG95_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY5890750.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            172..>340
                     /locus_tag="JQG95_004458"
     CDS             172..>340
                     /locus_tag="JQG95_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HAY5890751.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          109 a           63 c           82 g           86 t
ORIGIN      
        1 tgatgcttcg cactggtctg ggtgccagac aaattgaagc ataccgccag aactgttggg
       61 tggaaggctt ccacttcaaa cgagtatctc ctttagggaa gccagacagt aaacgaggga
      121 ttatctggta caactatccg aagataaatc agtttatcaa agactcatga tatgtctaaa
      181 ttaccaacag gtgtcgagat tcgaggtaaa tacattcgca tctggttcat gtttcgagga
      241 aaacgatgtc gggaaacatt gaaaggctgg gagattacta acagtaacat taaaaaggcc
      301 gggaatttaa gagcgttgat agttcatcaa atcagttccg
//
LOCUS       DADLCQ010000420          333 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000420 DADLCQ010000000
VERSION     DADLCQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.423"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>333
                     /locus_tag="JQG95_004459"
     CDS             <1..>333
                     /locus_tag="JQG95_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5890752.1"
                     /translation="ELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLFG
                     QSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIASK
                     PEFPGRKSS"
BASE COUNT          100 a           83 c           86 g           64 t
ORIGIN      
        1 tgagctgctt aaagccatgt tgcgtaagca acagagtcgg cttcgacagt atgcctgtca
       61 ggtcgcgggc tatgagcagg aaattgaacg gctgaaagcg caactcgaca ggttgcgtcg
      121 tatgttgttc ggccagagtt cagagaaaaa gcgtcataag cttgaaaatc agatccgaca
      181 ggcagaaaaa cgactgtcgg aactggaaaa ccggctgaac acagccagaa atcttctgga
      241 agatgcatcg tcagtcacag attcacctga caccagtccc ccgtcagaaa acccgatcgc
      301 cagtaagcct gaattcccgg gacgaaaatc ttc
//
LOCUS       DADLCQ010000421          323 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.424, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000421 DADLCQ010000000
VERSION     DADLCQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.424"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..272)
                     /locus_tag="JQG95_004460"
     rRNA            complement(<1..272)
                     /locus_tag="JQG95_004460"
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           96 c           65 g           88 t
ORIGIN      
        1 ggtgagccgt taccccacct actagctaat cccatctggg cacatccgat ggcaagaggc
       61 ccgaaggtcc ccctctttgg tcttgcgacg ttatgcggta ttagctaccg tttccagtag
      121 ttatccccct ccatcaggca gtttcccaga cattactcac ccgtccgcca ctcgtcagcg
      181 aaacagcaag ctgcttcctg ttaccgttcg acttgcatgt gttaggcctg ccgccagcgt
      241 tcaatctgag ccatgatcaa actcttcaat ttaaaagttt gatgctcaaa gaattaaact
      301 tcgtaatgaa ttacgtgttc act
//
LOCUS       DADLCQ010000422          317 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000422 DADLCQ010000000
VERSION     DADLCQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.425"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..269
                     /locus_tag="JQG95_004461"
     CDS             <1..269
                     /locus_tag="JQG95_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY5890753.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
BASE COUNT           82 a           86 c           88 g           61 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacag
//
LOCUS       DADLCQ010000423          317 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000423 DADLCQ010000000
VERSION     DADLCQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.426"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            52..>317
                     /locus_tag="JQG95_004462"
     CDS             52..>317
                     /locus_tag="JQG95_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5890754.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLL"
BASE COUNT           67 a           87 c           95 g           68 t
ORIGIN      
        1 cccggagtgt ggcacggttc actggccgga cggagtcgcc ccgcccttct gatgctcccc
       61 cgttttgccg atatttttca gcagggaaac cgctggctta actggctgga gaaacaaccg
      121 gaaggggcag tccgtccggt ggtaattgaa tcagtgacaa aaatcatggc ctgcgggacc
      181 acactgatgg gctacacgca gtggtgttgt tcatcaccgg actgttgcca tacaaaaaag
      241 gtctgcttcc ggtgtaaaag ccgctcctgc ccgcactgcg gggtgaaggc tggcgcacag
      301 tggatacagt atctgct
//
LOCUS       DADLCQ010000424          315 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000424 DADLCQ010000000
VERSION     DADLCQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.427"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>315
                     /locus_tag="JQG95_004463"
     CDS             <1..>315
                     /locus_tag="JQG95_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890755.1"
                     /translation="LRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYV
                     RDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEA
                     GC"
BASE COUNT           76 a           76 c          101 g           62 t
ORIGIN      
        1 aactccgtcc tctgtatata gcgctgaatg actatgttct ggaggcagga aaggtgcacg
       61 cagatgacac tccggtgaaa gtactggccc cggggaacgg aaagacgaaa acgggtcgtc
      121 tgtgggtata cgtcagggat gatcgtaatg cgggttcatc cctgccggca gccgtctggt
      181 tcgcgtattc ggcagatcgc aaaggagaac atccgcagct ccacctggca aagtatcagg
      241 gcgtactgca ggctgatgcc tatgcaggtt ataacgtact gtacgaaacg ggccgggtga
      301 aggaagccgg gtgcc
//
LOCUS       DADLCQ010000425          311 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000425 DADLCQ010000000
VERSION     DADLCQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.428"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>311
                     /locus_tag="JQG95_004464"
     CDS             <1..>311
                     /locus_tag="JQG95_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY5890756.1"
                     /translation="GGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQS
                     WLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVS
                     P"
BASE COUNT           90 a           79 c           79 g           63 t
ORIGIN      
        1 tgggcggcaa cagcaaaagt tcggcgctgt tcatcagcct gctgaagcgg cttaaagcga
       61 cataccgtcg tgcgaaaacc atcacgctga tcgtggacaa ctacattatc cacaaaagcc
      121 gggaaacaca gagctggctg aaggagaacc cgaagttcag gggcatttat cagccggttt
      181 actcgccatg ggtgaatcat gttgaacggc tatggcaggc acttcacgac acaataacgc
      241 gtaatcatca gtgccgctca atgtggcaac tgttgaaaaa agttcgccat tttatggaaa
      301 ccgtcagccc a
//
LOCUS       DADLCQ010000426          306 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000426 DADLCQ010000000
VERSION     DADLCQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.429"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            35..>306
                     /locus_tag="JQG95_004465"
     CDS             35..>306
                     /locus_tag="JQG95_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY5890757.1"
                     /translation="MLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVNKPAPSMPVIHD
                     VPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT"
BASE COUNT           88 a           74 c           64 g           80 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcac
//
LOCUS       DADLCQ010000427          305 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000427 DADLCQ010000000
VERSION     DADLCQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.430"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(55..243)
                     /locus_tag="JQG95_004466"
     CDS             complement(55..243)
                     /locus_tag="JQG95_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890758.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           74 a           75 c           93 g           63 t
ORIGIN      
        1 cgtgaacaga cgtttcagcg ggcggggagt aaaagcggac aacatgagga cggattaacc
       61 cccggtatcc tgtagccggg taagggctgc tgtgtcgcca cggcgctcca ggatggaggc
      121 cgcgaccatc gcaggtggtg ggtccatggg tttgagcatc ttcatccagt cccattcacg
      181 ttgtgagagc ttctcagcac caacagagag cagaacggta tctatacttt tgcgggtcaa
      241 cataagagga tgagtcctta ataaaacagg tcctcagagc atacatattc tgcggagcgt
      301 gcggc
//
LOCUS       DADLCQ010000428          292 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000428 DADLCQ010000000
VERSION     DADLCQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.431"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           96 a           57 c           54 g           85 t
ORIGIN      
        1 gttttctcta cttatcaagg gataattaat acgactcact atagggagaa gggacacaaa
       61 aagttttctc ttctaggaca actaaccgta acttatcgca tcctttcagg ggcgggatga
      121 ccaattacag acaccaagcg ttctacttaa agtgaatgct tcctgtgtgc tctgggtcag
      181 tcgataagtg aatagcaata agacacaatc atcttaatta tggtctctag tccctacagg
      241 ggcttaactt aaagttaact taaagacaca attaaagatt aacttatagt at
//
LOCUS       DADLCQ010000429          290 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000429 DADLCQ010000000
VERSION     DADLCQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.432"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>290)
                     /locus_tag="JQG95_004467"
     CDS             complement(<1..>290)
                     /locus_tag="JQG95_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY5890759.1"
                     /translation="WVGKDVTGIEPRNASVIEVPDITTNRRITAPGYWFYRNDEFVFD
                     YRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQA"
BASE COUNT           63 a           87 c           64 g           76 t
ORIGIN      
        1 cgcctgcagc gatttcgcgt aaatacgaca ggctttcagc ttttctttat cttcgtcgct
       61 gattaacccc agcagcaggt cttcttccca ttcccctgtc cgggcactga cctgagccag
      121 aagggcatca cgctcatctt ccgctttgag tctgtagtca aagacaaatt catcattgcg
      181 gtaaaaccag taacccggcg cggtaatccg tcggttagtg gtaatatccg gaacttcgat
      241 aacgctggca ttgcgtggct cgatgcctgt cacatcctta ccgacccaca
//
LOCUS       DADLCQ010000430          278 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000430 DADLCQ010000000
VERSION     DADLCQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.433"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            24..100
                     /locus_tag="JQG95_004468"
     tRNA            24..100
                     /locus_tag="JQG95_004468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JQG95_004469"
     tRNA            143..218
                     /locus_tag="JQG95_004469"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DADLCQ010000431          277 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000431 DADLCQ010000000
VERSION     DADLCQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.434"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            1..277
                     /locus_tag="JQG95_004470"
                     /pseudo
     CDS             1..277
                     /locus_tag="JQG95_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a           58 c           89 g           44 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgtt
//
LOCUS       DADLCQ010000432          275 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000432 DADLCQ010000000
VERSION     DADLCQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.435"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>275)
                     /locus_tag="JQG95_004471"
     CDS             complement(<1..>275)
                     /locus_tag="JQG95_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY5890760.1"
                     /translation="TEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVR
                     GQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCAL"
BASE COUNT           65 a           76 c           65 g           69 t
ORIGIN      
        1 acagggcgca atatgaccct ggtaatgaat gctctgaaca tcccgtaatg aaaaattgtg
       61 gggatgctat atacgttccg gtgggagtac tgggcactga aatccccacc ggtcccagtc
      121 gtgagcctct gtatgactgc cccctgacaa gtctgatgac tttatcaccg tcagcttctc
      181 cctggtacgc agcaataatc agcccgccaa tgtcagggtc tccccatctg ttgcggacag
      241 agctcgccac gattctgtaa ataatatctt ctgtg
//
LOCUS       DADLCQ010000433          275 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000433 DADLCQ010000000
VERSION     DADLCQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.436"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>275
                     /locus_tag="JQG95_004472"
     CDS             <1..>275
                     /locus_tag="JQG95_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890761.1"
                     /translation="VNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLR
                     PLGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTV"
BASE COUNT           63 a           79 c           75 g           58 t
ORIGIN      
        1 gtgaacaaaa caatgctaca ggccagactg gctgttgagc tgcaaatcct cagggatgaa
       61 ttactctcag gccactacca gcccttgccc gccagacggg tttacatccc taaaagcaac
      121 ggcaaactgc gaccactggg tatccccgcg ttgcgggatc gtattgttca gcgggccatg
      181 ctgatggcga tggagccgat atgggagagt gattttcata cgctctcata tggcttccgg
      241 cctgagcgca gtgtccacca cgcgatccgc acggt
//
LOCUS       DADLCQ010000434          274 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000434 DADLCQ010000000
VERSION     DADLCQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.437"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..244)
                     /locus_tag="JQG95_004473"
     CDS             complement(<1..244)
                     /locus_tag="JQG95_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY5890762.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPL"
BASE COUNT           62 a           75 c           63 g           74 t
ORIGIN      
        1 gcagtggtcg gtaatacaca ccgtcaacgt tatccgctga gttacggccg gtatccgata
       61 tcgccatccc tgacagcccg tgtcccgaag gtgcgcaaaa tctccacgaa acggtgttac
      121 tgccggggct gtaatacatt tcatggccat accgtacatc ctgtaccccc tctgttcgat
      181 atttatagcg ggcatcgaag tttccgtaat ttaacggaat tacctgtccg ttaacagtga
      241 acactatgct gttatccggg tttctctggc tgaa
//
LOCUS       DADLCQ010000435          274 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000435 DADLCQ010000000
VERSION     DADLCQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.438"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..185
                     /locus_tag="JQG95_004474"
     CDS             <1..185
                     /locus_tag="JQG95_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY5890763.1"
                     /translation="VLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRN
                     TYTSRFTEVAMAFASI"
BASE COUNT           70 a           65 c           84 g           55 t
ORIGIN      
        1 gggttttatt tggtaaaacc aatcacggca agctcagcgg cgaaatactg taccggttgg
       61 tggggcaagg caagaagctg ttccgctggc ggctacccga aggtaatccc tatctgagga
      121 cggagaccag aaacacgtat acatcgcgct ttacagaagt ggcaatggcg ttcgccagca
      181 tttaaatgga gagccggatg cgctgaaagg tgcacgtccg gttcggggag gagaggcagg
      241 gaaatagtcc gactacgccc tgcctcttac tcta
//
LOCUS       DADLCQ010000436          271 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000436 DADLCQ010000000
VERSION     DADLCQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.439"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>271
                     /locus_tag="JQG95_004475"
     CDS             <1..>271
                     /locus_tag="JQG95_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAY5890764.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLI"
BASE COUNT           55 a           78 c           59 g           79 t
ORIGIN      
        1 gtggctttta tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc
       61 gcatggcaac gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca
      121 ctggcctggg gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc
      181 ggtttgccca tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc
      241 tttgaactgc gtcatttata tcaactcatc a
//
LOCUS       DADLCQ010000437          270 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000437 DADLCQ010000000
VERSION     DADLCQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.440"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>270)
                     /locus_tag="JQG95_004476"
     CDS             complement(<1..>270)
                     /locus_tag="JQG95_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY5890765.1"
                     /translation="EGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNA
                     FVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATISAPEH"
BASE COUNT           76 a           51 c           62 g           81 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc
//
LOCUS       DADLCQ010000438          267 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.441, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000438 DADLCQ010000000
VERSION     DADLCQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.441"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           69 a           64 c           77 g           57 t
ORIGIN      
        1 acccttgtgc ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac
       61 atatccctgg cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa
      121 tgctcaggac gttgcagcgt tttgcgtgac cgctcgggga aggcaaaact gcttcaggga
      181 aagcgttgcg cggggtccgg cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg
      241 ggaaagcccc tcccgaggaa ggggcct
//
LOCUS       DADLCQ010000439          265 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000439 DADLCQ010000000
VERSION     DADLCQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.442"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>265
                     /locus_tag="JQG95_004477"
     CDS             <1..>265
                     /locus_tag="JQG95_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5890766.1"
                     /translation="GVDVGKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHG
                     QILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG"
BASE COUNT           64 a           75 c           64 g           62 t
ORIGIN      
        1 cggcgttgat gtcggtaaag atacgcatca cgctgttgcc attaatcgtt caggtaaacg
       61 cctgttcgat aaagcattac ccaacgacga aaacaaactc aggtcgctaa tatctgacct
      121 gaaacaacat ggtcagatac tgctggttgt tgatcagcca gctaccatcg gtgcgttacc
      181 tgtcgccgtt gcccgctcag aaggagtcct tgtcggatac ctccctggac tggccatgcg
      241 ccgcatagcc gacttacacg ccggt
//
LOCUS       DADLCQ010000440          261 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000440 DADLCQ010000000
VERSION     DADLCQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.443"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            101..>261
                     /locus_tag="JQG95_004478"
     CDS             101..>261
                     /locus_tag="JQG95_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAY5890767.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCAR"
BASE COUNT           75 a           76 c           62 g           48 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc g
//
LOCUS       DADLCQ010000441          255 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000441 DADLCQ010000000
VERSION     DADLCQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.444"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>255
                     /locus_tag="JQG95_004479"
     CDS             <1..>255
                     /locus_tag="JQG95_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY5890768.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           70 a           62 c           74 g           49 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgc
//
LOCUS       DADLCQ010000442          254 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000442 DADLCQ010000000
VERSION     DADLCQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.445"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>254
                     /locus_tag="JQG95_004480"
     CDS             <1..>254
                     /locus_tag="JQG95_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY5890769.1"
                     /translation="PDGRRAVLRSQVLELHGISHGSAGARSIATMATQRGYQMGRWLA
                     GRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVT"
BASE COUNT           60 a           71 c           79 g           44 t
ORIGIN      
        1 ccagacggca gacgggctgt attacgcagc caggtacttg aactgcatgg catcagccac
       61 ggctctgccg gagcaagaag catcgccaca atggcaaccc agagaggcta ccagatgggg
      121 cgctggcttg ctggcagact catgaaagag ctggggctgg tcagttgcca gcagccgact
      181 caccggtata agcgtggcgg tcatgagcac gttgctatcc cgaatcatct tgagcgacag
      241 ttcgccgtaa cgga
//
LOCUS       DADLCQ010000443          245 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000443 DADLCQ010000000
VERSION     DADLCQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.446"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>245
                     /locus_tag="JQG95_004481"
     CDS             <1..>245
                     /locus_tag="JQG95_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY5890770.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYM"
BASE COUNT           69 a           58 c           63 g           55 t
ORIGIN      
        1 cgcttatccg atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat
       61 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      121 cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat
      181 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      241 catgg
//
LOCUS       DADLCQ010000444          241 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000444 DADLCQ010000000
VERSION     DADLCQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.447"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..230
                     /locus_tag="JQG95_004482"
     CDS             <1..230
                     /locus_tag="JQG95_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY5890771.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT           66 a           53 c           69 g           53 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 a
//
LOCUS       DADLCQ010000445          223 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000445 DADLCQ010000000
VERSION     DADLCQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.448"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            25..180
                     /locus_tag="JQG95_004483"
                     /pseudo
     CDS             25..180
                     /locus_tag="JQG95_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT           75 a           49 c           51 g           48 t
ORIGIN      
        1 tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa
       61 ctggctaaag gcctcaaaac cgaggccgac ctcaatcagt tttcccgtat gcagacgaaa
      121 ttaactgtcg aaacggtgct taatgcggaa ctaactgacc atctcgggca tgaaaaaagc
      181 tacatccggt agtaaacatt atcagtggat tatgcgatat gat
//
LOCUS       DADLCQ010000446          221 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000446 DADLCQ010000000
VERSION     DADLCQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.449"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>221
                     /locus_tag="JQG95_004484"
     CDS             <1..>221
                     /locus_tag="JQG95_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY5890772.1"
                     /translation="MTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYD
                     WIQLQRKTLSKHAEMAKAFDYILNHWNAL"
BASE COUNT           67 a           45 c           69 g           40 t
ORIGIN      
        1 aaatgactca ggaagcgctc agacggatag cagagttata cgacatagaa gcggagatac
       61 gtggcagtcc ggcagaggaa cggcttgcag tcagaaaagc cagaagcgtc cagttgatgc
      121 agtcgttgta cgactggata cagttgcaga ggaaaacgct gtcgaaacat gcggagatgg
      181 cgaaggcgtt cgactatatc ctgaatcact ggaatgcgct g
//
LOCUS       DADLCQ010000447          220 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.450, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000447 DADLCQ010000000
VERSION     DADLCQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.450"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>220
                     /locus_tag="JQG95_004485"
     CDS             <1..>220
                     /locus_tag="JQG95_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY5890773.1"
                     /translation="GRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRIT
                     RLLSRKYPDLVMPDALAAEGSSKREWNRF"
BASE COUNT           53 a           54 c           67 g           46 t
ORIGIN      
        1 ggggcgtgac cagcagtggc atccgcatat ccatttatcg acaactgccg gtggtgtgac
       61 gtcgggccac acctggaaaa atcttcattt ttacgcccgt aaggtgatga gcatgtggcg
      121 ctaccggata acacggttgc tgtcacggaa ataccctgac ctggtgatgc cggatgcgct
      181 ggcagcagaa ggaagcagta aacgggaatg gaatcgcttc
//
LOCUS       DADLCQ010000448          218 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000448 DADLCQ010000000
VERSION     DADLCQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.451"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..>218)
                     /locus_tag="JQG95_004486"
     CDS             complement(<1..>218)
                     /locus_tag="JQG95_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY5890774.1"
                     /translation="RVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYA
                     GLAPVTRRSGSSIRGEHPSRRGNKALKR"
BASE COUNT           46 a           59 c           78 g           35 t
ORIGIN      
        1 ccgtttgaga gctttattac cccgtcgcga gggatgctca ccgcgtattg acgagccgga
       61 tcgccgagtt accggcgcaa ggccagcata agcagcgaga tgtgcggcag aggcgaaggc
      121 gcggcaggcg acctcggtga ggagtctggc tgcggtcctg acaccgactc cgggcatact
      181 ggtcaggacc gggtaaagag ggtgagcaag aactcgct
//
LOCUS       DADLCQ010000449          212 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000449 DADLCQ010000000
VERSION     DADLCQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.452"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           63 a           43 c           74 g           32 t
ORIGIN      
        1 ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg gctgaagaaa
       61 aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct aagtgtgccc
      121 gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac catgcagaga
      181 gagaaaacac cggagtggag ggaaaagcag aa
//
LOCUS       DADLCQ010000450          211 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.453, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000450 DADLCQ010000000
VERSION     DADLCQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.453"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            <1..>211
                     /locus_tag="JQG95_004487"
     CDS             <1..>211
                     /locus_tag="JQG95_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY5890775.1"
                     /translation="NWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGDFGY
                     QRSRWSTELLAIKINEITGCQLNAGT"
BASE COUNT           51 a           53 c           58 g           49 t
ORIGIN      
        1 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
       61 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      121 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      181 ataaccggtt gccagttaaa tgccggaacc g
//
LOCUS       DADLCQ010000451          210 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000451 DADLCQ010000000
VERSION     DADLCQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.454"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           83 a           30 c           22 g           75 t
ORIGIN      
        1 tggagtcatt accttaaatc aagcatacta aatccaataa gtgacaaatc tttaatttat
       61 agatttcatg aatatttaaa tattaactga acgagtaatt cattgattca acatctaatg
      121 actatccaac aaataaatat gcgtgatata taaagccctt tctttagttt attttcagaa
      181 aaagatacac aaaatttata tttggcattt
//
LOCUS       DADLCQ010000452          205 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000452 DADLCQ010000000
VERSION     DADLCQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.denovo.455"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
BASE COUNT           62 a           34 c           41 g           68 t
ORIGIN      
        1 gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat atatttctcc
       61 atctatgact tgtcaatact atcctgatat ttgtgggtca gcaatatatt ctactaaaag
      121 gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaaagcat gataaatata
      181 tcacgataac gttcagtatg tatcg
//
LOCUS       DADLCQ010000453        10615 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.guided.132, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000453 DADLCQ010000000
VERSION     DADLCQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.guided.132"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(<1..311)
                     /locus_tag="JQG95_004488"
     CDS             complement(<1..311)
                     /locus_tag="JQG95_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HAY5890776.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IG"
     gene            256..1041
                     /gene="lafU"
                     /locus_tag="JQG95_004489"
     CDS             256..1041
                     /gene="lafU"
                     /locus_tag="JQG95_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY5890777.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            1112..2167
                     /gene="dinB"
                     /locus_tag="JQG95_004490"
     CDS             1112..2167
                     /gene="dinB"
                     /locus_tag="JQG95_004490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY5890778.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            2219..2512
                     /gene="yafN"
                     /locus_tag="JQG95_004491"
     CDS             2219..2512
                     /gene="yafN"
                     /locus_tag="JQG95_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY5890779.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            2515..2913
                     /gene="yafO"
                     /locus_tag="JQG95_004492"
     CDS             2515..2913
                     /gene="yafO"
                     /locus_tag="JQG95_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY5890780.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            2923..3375
                     /locus_tag="JQG95_004493"
     CDS             2923..3375
                     /locus_tag="JQG95_004493"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY5890781.1"
                     /translation="MNNIQIRDYHPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            3621..4664
                     /locus_tag="JQG95_004494"
     CDS             3621..4664
                     /locus_tag="JQG95_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY5890782.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLGRPD"
     gene            4780..5340
                     /locus_tag="JQG95_004495"
                     /pseudo
     CDS             4780..5340
                     /locus_tag="JQG95_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5397..6854)
                     /gene="pepD"
                     /locus_tag="JQG95_004496"
     CDS             complement(5397..6854)
                     /gene="pepD"
                     /locus_tag="JQG95_004496"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY5890783.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7116..7574
                     /gene="gpt"
                     /locus_tag="JQG95_004497"
     CDS             7116..7574
                     /gene="gpt"
                     /locus_tag="JQG95_004497"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY5890784.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7666..8910
                     /gene="frsA"
                     /locus_tag="JQG95_004498"
     CDS             7666..8910
                     /gene="frsA"
                     /locus_tag="JQG95_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY5890785.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPKLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            8968..9369
                     /locus_tag="JQG95_004499"
     CDS             8968..9369
                     /locus_tag="JQG95_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY5890786.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(9408..10463)
                     /gene="phoE"
                     /locus_tag="JQG95_004500"
     CDS             complement(9408..10463)
                     /gene="phoE"
                     /locus_tag="JQG95_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY5890787.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GIIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         2646 a         2574 c         2714 g         2681 t
ORIGIN      
        1 ccgatacaga cgccgccgct caggttgatc gccagaatca tcatcccggc gatggcgtct
       61 ccgcgtacaa acttcgatgc cccgtccatc gcgccgtaga aatcggcctc gctggcaaca
      121 tctttacgcc gggtttgcgc ctgcgcctgg ttgatcaatc cggcgttaag atcggcgtca
      181 atcgccatct gtttgccggg catcgcgtcc agggtaaagc gggcagaaac ctcggaaata
      241 cgctcggcct ctttggtgac gacgataaag ttgattgtca acagcgtcag taaatccgag
      301 cgtgaaagca ttatcgccgc gctgcacggg cagtcgattt ttaacggtgg tgggctgtcg
      361 ccgttaaata aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa
      421 gagacggaga aaaaagcgcg tgatgtgaat gagaaaacgg cgctgctgaa gaagaaatcc
      481 gccaccgagc ttggcgagtt ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg
      541 gaagcgaatc tggagatgga gattgttccc cagggattac gcgtgctgat taaagacgac
      601 cagaatcgca atatgttcga acgcggcagc gcgcagatta tgccgttctt taaaactttg
      661 ctggtggagc tcgcgccagt gttcgactcg ctctataaca aaattattat taccgggcat
      721 accgatgcga tggcctacaa aaacaatatc tacaacaact ggaacctttc gggcgatcgc
      781 gcgctttcag ctcgtcgggt gctggaagag gccggaatgc cggaagataa agtgatgcag
      841 gtaagcgcaa tggcggacca gatgctgctg gacgcgaaga atccgcaaag cgcgggcaat
      901 cggcgtattg agattatggt gctgaccaaa agtgcgtccg atacgctgta tcaatacttt
      961 ggtcagcatg gggataaagt ggtgcagccg ctggtgcaaa agctggataa gcagcaggtg
     1021 ctttcgcagc gaatgcgcta aatgctgaat ctttacgcat ttctcaaaac ctgaaatcac
     1081 tgtatacttt accagtgttg agaggtgagc aatgcgtaaa atcattcatg tggatatgga
     1141 ctgctttttc gcagcggtgg agatgcgcga caatcccgct ctgcgcgata tccctattgc
     1201 tattggcggc agccgcgaac gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa
     1261 atttggcgta cgtagcgcta tgcctactgg gatggcgctc aaattatgcc cacatctcac
     1321 cttgcttccg gggcgctttg acgcctacaa agaagcctca aatcatatcc gtgaaatctt
     1381 ctcgcgctac acctcgcgca ttgaaccgtt gtcactggat gaggcttatc tcgatgtcac
     1441 cgatagcgtc cattgccacg gttctgcgac cctcatcgcc caggaaatcc gccagacgat
     1501 cttcaacgag ctgcaactga cggcatctgc gggcgtggca ccagtaaagt ttctcgccaa
     1561 aatcgcctcc gacatgaata aacccaacgg tcagtttgtg attacgccgg cagaagttcc
     1621 ggcattttta caaaccttac cactggaaaa aatccccggc gtcggcaaag tctcggcggc
     1681 aaaactggaa gcgatggggc tgcggacctg cggtgatgta caaaagtgtg atctggtgat
     1741 tctgcttaaa cgctttggca aatttggccg cattttgtgg gagcgtagtc aggggattga
     1801 cgagcgcgac gttaacagcg aacggttacg aaaatccgtc ggcgtggaac gcacgatggc
     1861 ggaagatatt catcactggt ctgaatgtga agcgattatc gagcggctgt atccggaact
     1921 tgaacgccgt ctggcaaagg taaaacctga tttactgatt gctcgccagg gggtgaaatt
     1981 aaagttcgac gattttcagc agaccaccca ggagcacgtc tggccgcggc tgaataaagc
     2041 tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg
     2101 tctggtgggg ctgcatgtga cgttgcttga cccgcaaatg gaaagacaac tggtgctggg
     2161 attatgatgt atactattat gtatattctg gtgtgcatta ttatgagggt atcactgtat
     2221 gcatcgaatt ctcgctgaaa aatcggtcaa tatcactgag ttacgtaaaa acccagctaa
     2281 atactttatt gatcaaccag ttgcggttct ttctaataat cgccccgcag gatatctctt
     2341 aagtgccagc gcattcgaag cgttaatgga catgcttgct gaacaagagg agaaaaagcc
     2401 cataaaggcg cgctttcgtc caagtgctgc aagattagag gaaattacac gccgcgctaa
     2461 acaatatctt aatgatatga cggatgatga tttcaatgac tttaaggaat aaggatgcgg
     2521 gtattcaaaa caaaacttat tcgcctgcaa cttacagcag aggaacttga tgcgttaacg
     2581 gcggatttta tttcctataa gcgtgacggt gttttgccag atatatttgg tcgcgatgca
     2641 ctctacgacg actcgtttac ctggccatta atcaaatttg agcgagttgc tcatattcat
     2701 ctggcaaatg tgaataatcc atttccgcca cagttgcgcc aattcagcag aacgaatgac
     2761 gaagcgcatt tggtatattg tcagggtgcg tttgatgagc aagcatggtt gctcattgcc
     2821 attctgaagc ctgaacctca taaactggct cgagataaca accaaatgca taaaattgga
     2881 aaaatggcag aagcgtttcg catgcgtttt tgaatttata ttatgaataa catacaaata
     2941 agagattatc atcctggcga ttttcagcaa ctatgcgcta ttttcattag agcggttacg
     3001 atgaccgtca gtcagcatta ttcactacaa caaattgccg cctgggcgca gattgacgaa
     3061 tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagttat taatgcaaaa
     3121 ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt tgaccctgaa
     3181 tacacccgcc gtggggttgc cagcgctttg ttaaaacctt ttattaagtc tgaatccgaa
     3241 cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg ttttcagaca
     3301 gttaagcagc agcgcgttga atgccgggga gcgtggttta ttaattttta tatgcgttat
     3361 aaaccgcaac attaaatcca gcttgcaatg aaaataacgc ccgcctagta tgtgcgcgtt
     3421 ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca atgactcttt
     3481 gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc catttcctga
     3541 ttttttaatt gttactctca ataattcaag tcgcagcaca tgcaacttga agtatgacga
     3601 gtctatttac ggaatggtat atgggcaatt atattcgtcc cttatccgat gcggtattta
     3661 ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta cacaccacgg
     3721 caaatttacc caacatgcag cgcgtagtgg ggatgccaga tttacacccc ggacgcggct
     3781 acccgattgg cgcagcgttc ttctccgcag gccgttttta cccagcactg gtcggcaatg
     3841 atatcggctg tggcatggcg ctatggcaaa cagatattct cgctcgcaaa tacaacgccg
     3901 ataagtttga aaagcgatta tcagctttgg atgacgttgc tgaagaaagc tggctggagg
     3961 aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc tctatcggtg
     4021 gtggcaatca tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct gaactgtttg
     4081 cactggcagg tctggatgcg cagcatctgc aactgctggt tcatagcggc tcgagggggc
     4141 tagggcagtc tattttacag cggcatattg cctcgttttc gcatcatggt ttgcctgaag
     4201 gcagtgacga cgcgctggcg tttgcgcgtt ttaatcgcca cctgatcgct ttgcgcataa
     4261 tgcaacaggt taaggccacc ggaagtccgg ttctggatgt ggcgcataac tttgttagcg
     4321 cgtgtcgaat cggtgatcaa caaggggtct tacatcgtaa aggtgccacg cctgatgact
     4381 gtggtctggt ggttattccc ggttcacgcg gtgattactc caggctggtt cagcccgtca
     4441 ggagtgagga aacattgcat tcgctggcgc atggggctgg gcgtaaatgg gggcgcaccg
     4501 agtgtaaagg gcgtctggca gcgaaataca cagcgacgca actctcgcgt actgaacttg
     4561 gcagtcgggt aatttgtcgc gataaacaac tcatcttcga agaagcgcca caagcctata
     4621 aatcggctga aagtgtggtg caatgtctgg gcaggccgga ttaattattc ctgtcgcgcg
     4681 gctgcgtccg gtgctaacgc tcaaaaacag tggagggaaa aaaggatgat cttgctataa
     4741 ctctcctcag cgcaggggcc ggaagaatgt tgtctcgcag tgaaaaaggc gctggacagg
     4801 ctaattaaag aagctgcccg acaagacgtc gcggtaacgg tgctggaaac agaaacaggc
     4861 cgctactctg acacgctgcg ttcggcgctg atttctctgg atggcgacaa cgcatgggcg
     4921 ttaagcgaaa gctggtgtgg cactattcag tggatttgcc cgagcccgta tcgcccacat
     4981 cacgggcgta aaaactggtt tctgggcatt gggcgtttta ccgctgatga gcaggagcaa
     5041 tccgatgcga tccggtatga gacgctgcgt tcgtcggggc cgggcggtca acatgtcaat
     5101 aaaaccgact cggcggtacg cgccacgcat ttggcatccg gtattagcgt gaaggttcag
     5161 tcagagcgca gccagcatgc taacaaacgg ctggcgcgat tgctgattgc ctggaagctg
     5221 gatcagcagc aataggaaaa tagcgcggtg ctgaaatcac agcggcgaat gttccatcac
     5281 cagattgaac gtggcaaccc gcgacgaacg tttacaggga tggcttttat cgaaggataa
     5341 tgaaggatga aactgccgga aggcgattaa acgccatccg gcagcaaatc aaataattac
     5401 ttcgccggaa tttctttcag cagttcagtc agcagtgtcc agtaatgacc tacgctttcg
     5461 atgtgaactt gctcatccgg agagtgtgga ccggtgatag ttggcccgat agaaaccatg
     5521 tccatttccg gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg
     5581 atgttcggcg tcttgttgaa caggcgctga taggtttcac gtaccagatg catcaccgga
     5641 gaattagcgt ccggctgcca gccaggatat gcgcctttcg cttcggtttt cgcgccagcc
     5701 agtttaccca gcgaatccag catgctcacc acgtagtctt taccgctgtc gatcagtgaa
     5761 cggatcaggc agtgaatttc tacgttattg tcagtcatgg tcaccacacc gacgttcagg
     5821 gaggtttcga ccacaccttt tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg
     5881 ttcagcagac gaataaaggt atcgcgagat ttcgcaatca gggcagcttt atcgttcgct
     5941 acagagtcca gcaacaaggc cagatttttc tctttctctg ccagctcgtt tttcaggatc
     6001 tcctgatagg tattcaccag agatttcagt gcgtcgactt tatcagctgc gacagcaatg
     6061 gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg
     6121 cgcaggtcca gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta
     6181 cccaggccaa cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaac
     6241 ttgaaggttt caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag
     6301 tcgatccccc ccgcacaacc catgtagatt tcaccttctt cttcggagtc ggtgttaatc
     6361 agaatatcag cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct
     6421 tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc
     6481 gccagtgcag aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta
     6541 acccattcgc catcaatata aggctggata ggatctttcg tgaagtcatg cacggtgtcg
     6601 ttatttttct gcggcaccat atcgaggtgg gcctgtaaga cgaccggttt acgattttcc
     6661 atacctgcgg tagcaggttt acgaatcagg atattaccta cctgatcgcg ttcgacatgg
     6721 aaacctttct cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac
     6781 gggtgaggaa tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga
     6841 gacagttcag acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac
     6901 agggttagca gaaaatgttg tcaacacaag acaggcttgc gagatatgta ttgagaatac
     6961 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     7021 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     7081 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     7141 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     7201 ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg
     7261 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     7321 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     7381 acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca aaagcgcact
     7441 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     7501 tcccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     7561 tctccggtcg ctaatctttt caacgcctgg cattgccggg cgttgttctt tttaacttca
     7621 ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     7681 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     7741 cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa aaccatccct
     7801 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga cccacgtgaa
     7861 atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga cgatgattta
     7921 tacgataccg tgattggcta ccgtggtggc aactggattt atgagtgggc cacccaggcg
     7981 atggtgtggc aacaaaaagc ctgtacggaa gaagatccga aactcagtgg tcgacactgg
     8041 ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg agatgacctg
     8101 gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca gcgtctgccg
     8161 ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac cggctttttg
     8221 catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg tctggatgcg
     8281 atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg
     8341 ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc
     8401 agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt ggatcacact
     8461 cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt
     8521 gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac cctgttgagt
     8581 gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc gagtcgtttg
     8641 gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa
     8701 gtgcaaggat tgctggggcg tcgctgccca acgccaatgt tatcaggcta ctggaagaac
     8761 gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc tgacggtaaa
     8821 ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct tcaggaaatc
     8881 accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca atttggtaaa
     8941 acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc gaagagcaga
     9001 ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg cgaagataat
     9061 cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt
     9121 gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac ctacagctac
     9181 cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa agatactgaa
     9241 gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg tgaactgctg
     9301 acgacgctga atctgaagct ggaaccggcg aatgattttc gtgatgaacc ggtgaagtta
     9361 acggcgtgag tgaaatgtgc cggatgcatc acatccggca atattcatta aaactgatac
     9421 gtcatcccaa ccgcgacagt atcatcatta ttaatattca gcttattatc gctatccagt
     9481 tggttgattt tataatcaac aaacgctgac atatttttgt tgaaataata cgtagcaccg
     9541 acgtcgatat aattgaccag atcttcatca ccgatgccct caatatcttt ccctttcgat
     9601 aagacataac ccagcgatgg acgcagacca aagtcaaact ggtattgagc gaccgcttca
     9661 aagttctgtg tcttattggc aaagccgcca gttattggcg tcattttgcg tgtttcagaa
     9721 tagaaagttg ccagataaat attattggca tcgtatttca gacctgttgc ccatgcttct
     9781 gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc tgagttggta
     9841 taggccccac taatggcgaa atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag
     9901 ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt
     9961 aagttcaggc catcgataat gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg
    10021 gcgcgtttgg tcataaagtt gtcggtctgc gccgaggaat cgccaccaaa ttccgggaac
    10081 atatcggtcc aggcttccac gtcatacaac gcgcccaggt tacgaccata atcgaaagaa
    10141 cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg ttttttgctg tgcagtatca
    10201 ctctccgctt tatttccggc aaactccgct tcccaccgac cataaccagt cagttgatcg
    10261 ttaatttgag tttcaccttt aaaaccaaaa cggatataac tctggtcgcc atctttactg
    10321 gcgttatcac tcatataatg catggcttta actttgccat agacatccag tttattaccg
    10381 tctttattat atatttctgc agcctgtaca gatgcagatg ccacaatgcc catcaccact
    10441 aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc gaatattcag
    10501 cgggagagcc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa gatatattac
    10561 agattaatga tattctgaaa atgggtaatg ctgccaactt actgatttag tgtat
//
LOCUS       DADLCQ010000454         9721 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.guided.141, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000454 DADLCQ010000000
VERSION     DADLCQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.guided.141"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            complement(16..204)
                     /locus_tag="JQG95_004501"
     CDS             complement(16..204)
                     /locus_tag="JQG95_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY5890788.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JQG95_004502"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JQG95_004502"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY5890789.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="JQG95_004503"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="JQG95_004503"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY5890790.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTIPTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3526..4260
                     /locus_tag="JQG95_004504"
     CDS             3526..4260
                     /locus_tag="JQG95_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY5890791.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="JQG95_004505"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="JQG95_004505"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY5890792.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="JQG95_004506"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="JQG95_004506"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY5890793.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5099..6232
                     /gene="blaEC"
                     /locus_tag="JQG95_004507"
     CDS             5099..6232
                     /gene="blaEC"
                     /locus_tag="JQG95_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY5890794.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /locus_tag="JQG95_004508"
     CDS             6321..6854
                     /locus_tag="JQG95_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY5890795.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /locus_tag="JQG95_004509"
     CDS             complement(6851..7168)
                     /locus_tag="JQG95_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY5890796.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="JQG95_004510"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="JQG95_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY5890797.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="JQG95_004511"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="JQG95_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY5890798.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="JQG95_004512"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="JQG95_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY5890799.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="JQG95_004513"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="JQG95_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY5890800.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2476 a         2486 c         2472 g         2287 t
ORIGIN      
        1 gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accaactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg cagatccgtg
     2281 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatcc
     2641 ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta gtgaataatt
     6421 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
     6541 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata acactcggcg
     7081 tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg aaatttgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca aacggcacga
     7321 cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgagaga
     7621 tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcattgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg cctacatcgt
     9481 gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt tacgccgcat
     9541 ccggcaagag caacaaacgc aagtacagca acctgtcatt aaactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt cttttcggta atgactccaa cttactgata gtgttttatg
     9721 t
//
LOCUS       DADLCQ010000455         5663 bp    DNA     linear   BCT 05-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri isolate MH170026
            SAMEA104020417-rid11427633.guided.197, whole genome shotgun
            sequence.
ACCESSION   DADLCQ010000455 DADLCQ010000000
VERSION     DADLCQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA104020417
            Sequence Read Archive: ERR1948360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 29-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000943592.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000943592.1
            Genome Coverage       :: 54x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/29/2021 18:19:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,464
            Genes (coding)                    :: 4,104
            CDSs (with protein)               :: 4,104
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 47
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 360
            CDSs (without protein)            :: 360
            Pseudo Genes (ambiguous residues) :: 0 of 360
            Pseudo Genes (frameshifted)       :: 170 of 360
            Pseudo Genes (incomplete)         :: 81 of 360
            Pseudo Genes (internal stop)      :: 148 of 360
            Pseudo Genes (multiple problems)  :: 36 of 360
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA104020417-rid11427633.guided.197"
                     /isolate="MH170026"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Ireland"
                     /collection_date="2017"
     gene            69..284
                     /locus_tag="JQG95_004514"
     CDS             69..284
                     /locus_tag="JQG95_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY5890801.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            277..663
                     /locus_tag="JQG95_004515"
     CDS             277..663
                     /locus_tag="JQG95_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY5890802.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            671..1357
                     /locus_tag="JQG95_004516"
     CDS             671..1357
                     /locus_tag="JQG95_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY5890803.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="JQG95_004517"
     CDS             complement(1335..1958)
                     /gene="tetR(B)"
                     /locus_tag="JQG95_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY5890804.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2040..3245
                     /gene="tet(B)"
                     /locus_tag="JQG95_004518"
     CDS             2040..3245
                     /gene="tet(B)"
                     /locus_tag="JQG95_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY5890805.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="JQG95_004519"
     CDS             complement(3358..3951)
                     /gene="tetC"
                     /locus_tag="JQG95_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY5890806.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4039..4449
                     /locus_tag="JQG95_004520"
                     /pseudo
     CDS             4039..4449
                     /locus_tag="JQG95_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4465..>5663)
                     /locus_tag="JQG95_004521"
     CDS             complement(4465..>5663)
                     /locus_tag="JQG95_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HAY5890807.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1568 a         1044 c         1230 g         1821 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg accactatta agtcatgtag
       61 ctggttttat ttcaattgaa cgttttcaaa gtctagctac agaaggaaaa atgttatcgc
      121 tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga
      181 aagctcaaca agaagggcga gagtcaatat ttgattttta caaaattagt attgctcata
      241 ttactcgcga atattcattt aaaaaggaca aggataatgt ttgatgttca cgttgtttta
      301 gataatcaaa taggacaatt agcattacta ggaaaaacat taggtaataa aggtattgga
      361 ttggaagggg gagggatatt tacggttggt gatgaatgcc atgctcattt tcttgttgaa
      421 caaggaaagg aagctaaaat agcgctagag caagctggac tgttagtact tgcgatccgg
      481 acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg aacttggcga aatagcacga
      541 gtattggcgg agaataacat taatatttta gtgcaataca gtgaccatgc taaccaactg
      601 atattaataa cggacaatga tagtatggct gcatctgtta cgctcccttg ggcaataaag
      661 tgaacttgcg atggctaatt taatacgaaa agaggttacc tttgagtcct caatagccgc
      721 gataggggcg gctatgtctg acatttcacg agttaaaata ctcagtgctt tgatggatgg
      781 gcgagcttgg acggccactg agctaagttc tgtggcgaat atatcagctt caacggcgag
      841 cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc aaggcaagca
      901 tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta tgatggggat
      961 ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac gaaaagcacg
     1021 tacttgctat gatcatttag ctggcgaagt tgccgttaag atctatgatt ccctttgtca
     1081 acagcaatgg atcactgaaa atggttcaat gatcacatta agtggtattc aatattttca
     1141 tgaaatggga attgacgttc cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga
     1201 ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga gccgcattat tttcgcttta
     1261 tgaatctaaa gggtggttaa ctcgacatct tggttaccgt gaagttacca tcacggaaaa
     1321 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1381 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1441 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1501 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1561 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1621 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     1681 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     1741 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     1801 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     1861 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     1921 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1981 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaaa
     2041 tgaatagttc gacaaagatc gcattggtaa ttacgttact cgatgccatg gggattggcc
     2101 ttatcatgcc agtcttgcca acgttattac gtgaatttat tgcttcggaa gatatcgcta
     2161 accactttgg cgtattgctt gcactttatg cgttaatgca ggttatcttt gctccttggc
     2221 ttggaaaaat gtctgaccga tttggtcggc gcccagtgct gttgttgtca ttaataggcg
     2281 catcgctgga ttacttattg ctggcttttt caagtgcgct ttggatgctg tatttaggcc
     2341 gtttgctttc agggatcaca ggagctactg gggctgtcgc ggcatcggtc attgccgata
     2401 ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt aggggcaagt tttgggcttg
     2461 gtttaatagc ggggcctatt attggtggtt ttgcaggaga gatttcaccg catagtccct
     2521 tttttatcgc tgcgttgcta aatattgtcg ctttccttgt ggttatgttt tggttccgtg
     2581 aaaccaaaaa tacacgtgat aatacagata ccgaagtagg ggttgagacg caatcgaatt
     2641 cggtatacat cactttattt aaaacgatgc ccattttgtt gattatttat ttttcagcgc
     2701 aattgatagg ccaaattccc gcaacggtgt gggtgctatt taccgaaaat cgttttggat
     2761 ggaatagcat gatggttggc ttttcattag cgggtcttgg tcttttacac tcagtattcc
     2821 aagcctttgt ggcaggaaga atagccacta aatggggcga aaaaacggca gtactgctcg
     2881 gatttattgc agatagtagt gcatttgcct ttttagcgtt tatatctgaa ggttggttag
     2941 ttttccctgt tttaatttta ttggctggtg gtgggatcgc tttacctgca ttacagggag
     3001 tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt acagggatta ttggtgagcc
     3061 ttaccaatgc aaccggtgtt attggcccat tactgtttgc tgttatttat aatcattcac
     3121 taccaatttg ggatggctgg atttggatta ttggtttagc gttttactgt attattatcc
     3181 tgctatcgat gaccttcatg ttaacccctc aagctcaggg gagtaaacag gagacaagtg
     3241 cttagttatt tcgtcaccaa atgatgttat tccgcgaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttacg ataaagaaga tttagcttca aataaaacct atctatttta
     3361 tttatctttc aagctcaata aaaagccgcg gtaaatagca ataaattggc cttttttatc
     3421 ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa
     3481 gtttttaagc acatcatcat cataagcttt aagttggttc tcttggatca atttgctgac
     3541 aatggcgttt accttaccag taatgtattc aaggctaatt ttttcaagtt cattccaacc
     3601 aatgataggc atcacttctt ggatagggat aaggttttta ttattatcaa taatataatc
     3661 aagataatgt tcaaatatac tttctaaggc agaccaacca tttgttaaat cagtttttgt
     3721 tgtgatgtag gcatcaatca taattaattg ctgcttataa caggcactga gtaattgttt
     3781 tttattttta aagtgatgat aaaaggcacc tttggtcacc aacgcttttc ccgagatctc
     3841 atctattgaa acagcttgat agcctttttc aacaaacaat attcgtgctg agttaaccag
     3901 tgattgatag gtactcttaa aattttcttg ttgatgattt ttattttcca tgatagattt
     3961 aaaataacat accgtcagta tgtttatggt atcatgatga tgtggtcgtg acaatcttaa
     4021 gaacatttag gttattttat gtatattgaa cagcattctc gctatcaaaa taaagctaat
     4081 aacatccaat taagatatga tgataagcag tttcatacaa cggttatcaa agatgttcta
     4141 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
     4201 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
     4261 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
     4321 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcga
     4381 tttaagtaca tttttaaggt tacaccaagt tattatcggc gtaataaatt atgggaattg
     4441 gaggcaatgc actgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
     4501 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
     4561 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
     4621 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
     4681 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
     4741 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
     4801 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
     4861 taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt taggtgacgg
     4921 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
     4981 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
     5041 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
     5101 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
     5161 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
     5221 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
     5281 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
     5341 tcgcaatacc ataagtcgtt tttgctcacg aatatcagac cagtcaacaa gtacaatggg
     5401 catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt
     5461 gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc
     5521 tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt caagtaatgc
     5581 gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggt aatgactcca
     5641 acttactgat agtgttttat gtt
//
